LOCUS       DAVLSL010000001        45428 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLSL010000001 DAVLSL010000000
VERSION     DAVLSL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45428
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.001"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(133..591)
                     /locus_tag="VXN30_000001"
     CDS             complement(133..591)
                     /locus_tag="VXN30_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1356696.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(588..1028)
                     /locus_tag="VXN30_000002"
     CDS             complement(588..1028)
                     /locus_tag="VXN30_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1356697.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1234..3183
                     /gene="mutL"
                     /locus_tag="VXN30_000003"
     CDS             1234..3183
                     /gene="mutL"
                     /locus_tag="VXN30_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1356698.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3508..3975)
                     /locus_tag="VXN30_000004"
     CDS             complement(3508..3975)
                     /locus_tag="VXN30_000004"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1356699.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(3976..5181)
                     /locus_tag="VXN30_000005"
     CDS             complement(3976..5181)
                     /locus_tag="VXN30_000005"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1356700.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5201..5836)
                     /gene="ribE"
                     /locus_tag="VXN30_000006"
     CDS             complement(5201..5836)
                     /gene="ribE"
                     /locus_tag="VXN30_000006"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1356701.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5821..6921)
                     /gene="ribD"
                     /locus_tag="VXN30_000007"
     CDS             complement(5821..6921)
                     /gene="ribD"
                     /locus_tag="VXN30_000007"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1356702.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNGAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7056..7170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7327..7920
                     /gene="ruvA"
                     /locus_tag="VXN30_000008"
     CDS             7327..7920
                     /gene="ruvA"
                     /locus_tag="VXN30_000008"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1356703.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASVENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7930..8493
                     /locus_tag="VXN30_000009"
     CDS             7930..8493
                     /locus_tag="VXN30_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1356704.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPVKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8490..9167
                     /locus_tag="VXN30_000010"
                     /pseudo
     CDS             8490..9167
                     /locus_tag="VXN30_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(9308..10339)
                     /locus_tag="VXN30_000011"
     CDS             complement(9308..10339)
                     /locus_tag="VXN30_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1356705.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEEPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDEIFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10594..11277)
                     /locus_tag="VXN30_000012"
     CDS             complement(10594..11277)
                     /locus_tag="VXN30_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1356706.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPH
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11289..12233)
                     /locus_tag="VXN30_000013"
     CDS             complement(11289..12233)
                     /locus_tag="VXN30_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1356707.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12365..15196
                     /gene="uvrA"
                     /locus_tag="VXN30_000014"
     CDS             12365..15196
                     /gene="uvrA"
                     /locus_tag="VXN30_000014"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1356708.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKNVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMTAFGVDMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVVTNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15189..16250
                     /locus_tag="VXN30_000015"
     CDS             15189..16250
                     /locus_tag="VXN30_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1356709.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16382..16780
                     /gene="spx"
                     /locus_tag="VXN30_000016"
     CDS             16382..16780
                     /gene="spx"
                     /locus_tag="VXN30_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1356710.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16846..17415
                     /locus_tag="VXN30_000017"
     CDS             16846..17415
                     /locus_tag="VXN30_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1356711.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17502..17768
                     /locus_tag="VXN30_000018"
     CDS             17502..17768
                     /locus_tag="VXN30_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1356712.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17772..18191
                     /gene="ruvX"
                     /locus_tag="VXN30_000019"
     CDS             17772..18191
                     /gene="ruvX"
                     /locus_tag="VXN30_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1356713.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18207..18512
                     /locus_tag="VXN30_000020"
     CDS             18207..18512
                     /locus_tag="VXN30_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1356714.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18972..20222
                     /locus_tag="VXN30_000021"
     CDS             18972..20222
                     /locus_tag="VXN30_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1356715.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISIFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20219..20749
                     /locus_tag="VXN30_000022"
     CDS             20219..20749
                     /locus_tag="VXN30_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1356716.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTG
                     QDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIV
                     VDDMDRDPSDQIVLTK"
     gene            20895..22427
                     /gene="cls"
                     /locus_tag="VXN30_000023"
     CDS             20895..22427
                     /gene="cls"
                     /locus_tag="VXN30_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1356717.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22685..24223
                     /locus_tag="VXN30_000024"
     CDS             22685..24223
                     /locus_tag="VXN30_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1356718.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSLRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24343..26550
                     /gene="nrdD"
                     /locus_tag="VXN30_000025"
     CDS             24343..26550
                     /gene="nrdD"
                     /locus_tag="VXN30_000025"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1356719.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26562..26702
                     /locus_tag="VXN30_000026"
     CDS             26562..26702
                     /locus_tag="VXN30_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356720.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26746..27252
                     /locus_tag="VXN30_000027"
     CDS             26746..27252
                     /locus_tag="VXN30_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1356721.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            27245..27835
                     /gene="nrdG"
                     /locus_tag="VXN30_000028"
     CDS             27245..27835
                     /gene="nrdG"
                     /locus_tag="VXN30_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1356722.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27832..28449
                     /locus_tag="VXN30_000029"
     CDS             27832..28449
                     /locus_tag="VXN30_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1356723.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28715..29023
                     /gene="rpsJ"
                     /locus_tag="VXN30_000030"
     CDS             28715..29023
                     /gene="rpsJ"
                     /locus_tag="VXN30_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1356724.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29240..29866
                     /gene="rplC"
                     /locus_tag="VXN30_000031"
     CDS             29240..29866
                     /gene="rplC"
                     /locus_tag="VXN30_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1356725.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29891..30514
                     /gene="rplD"
                     /locus_tag="VXN30_000032"
     CDS             29891..30514
                     /gene="rplD"
                     /locus_tag="VXN30_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1356726.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30514..30810
                     /locus_tag="VXN30_000033"
     CDS             30514..30810
                     /locus_tag="VXN30_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1356727.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30828..31661
                     /gene="rplB"
                     /locus_tag="VXN30_000034"
     CDS             30828..31661
                     /gene="rplB"
                     /locus_tag="VXN30_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1356728.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31765..32046
                     /gene="rpsS"
                     /locus_tag="VXN30_000035"
     CDS             31765..32046
                     /gene="rpsS"
                     /locus_tag="VXN30_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1356729.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32058..32402
                     /gene="rplV"
                     /locus_tag="VXN30_000036"
     CDS             32058..32402
                     /gene="rplV"
                     /locus_tag="VXN30_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1356730.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32415..33068
                     /gene="rpsC"
                     /locus_tag="VXN30_000037"
     CDS             32415..33068
                     /gene="rpsC"
                     /locus_tag="VXN30_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1356731.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33072..33485
                     /gene="rplP"
                     /locus_tag="VXN30_000038"
     CDS             33072..33485
                     /gene="rplP"
                     /locus_tag="VXN30_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1356732.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33495..33701
                     /locus_tag="VXN30_000039"
     CDS             33495..33701
                     /locus_tag="VXN30_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1356733.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33726..33986
                     /gene="rpsQ"
                     /locus_tag="VXN30_000040"
     CDS             33726..33986
                     /gene="rpsQ"
                     /locus_tag="VXN30_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1356734.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            34012..34380
                     /gene="rplN"
                     /locus_tag="VXN30_000041"
     CDS             34012..34380
                     /gene="rplN"
                     /locus_tag="VXN30_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1356735.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34458..34763
                     /gene="rplX"
                     /locus_tag="VXN30_000042"
     CDS             34458..34763
                     /gene="rplX"
                     /locus_tag="VXN30_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1356736.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34787..35329
                     /gene="rplE"
                     /locus_tag="VXN30_000043"
     CDS             34787..35329
                     /gene="rplE"
                     /locus_tag="VXN30_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1356737.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35347..35616
                     /gene="rpsN"
                     /locus_tag="VXN30_000044"
     CDS             35347..35616
                     /gene="rpsN"
                     /locus_tag="VXN30_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1356738.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35901..36299
                     /gene="rpsH"
                     /locus_tag="VXN30_000045"
     CDS             35901..36299
                     /gene="rpsH"
                     /locus_tag="VXN30_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1356739.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36491..37027
                     /gene="rplF"
                     /locus_tag="VXN30_000046"
     CDS             36491..37027
                     /gene="rplF"
                     /locus_tag="VXN30_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1356740.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37111..37467
                     /gene="rplR"
                     /locus_tag="VXN30_000047"
     CDS             37111..37467
                     /gene="rplR"
                     /locus_tag="VXN30_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1356741.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37485..37979
                     /gene="rpsE"
                     /locus_tag="VXN30_000048"
     CDS             37485..37979
                     /gene="rpsE"
                     /locus_tag="VXN30_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1356742.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37951..38175
                     /gene="rpmD"
                     /locus_tag="VXN30_000049"
     CDS             37951..38175
                     /gene="rpmD"
                     /locus_tag="VXN30_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1356743.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38320..38760
                     /gene="rplO"
                     /locus_tag="VXN30_000050"
     CDS             38320..38760
                     /gene="rplO"
                     /locus_tag="VXN30_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1356744.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38773..40083
                     /gene="secY"
                     /locus_tag="VXN30_000051"
     CDS             38773..40083
                     /gene="secY"
                     /locus_tag="VXN30_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1356745.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40234..40872
                     /locus_tag="VXN30_000052"
     CDS             40234..40872
                     /locus_tag="VXN30_000052"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1356746.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40989..41207
                     /gene="infA"
                     /locus_tag="VXN30_000053"
     CDS             40989..41207
                     /gene="infA"
                     /locus_tag="VXN30_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1356747.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41232..41348
                     /gene="rpmJ"
                     /locus_tag="VXN30_000054"
     CDS             41232..41348
                     /gene="rpmJ"
                     /locus_tag="VXN30_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1356748.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41366..41731
                     /gene="rpsM"
                     /locus_tag="VXN30_000055"
     CDS             41366..41731
                     /gene="rpsM"
                     /locus_tag="VXN30_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1356749.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41749..42132
                     /gene="rpsK"
                     /locus_tag="VXN30_000056"
     CDS             41749..42132
                     /gene="rpsK"
                     /locus_tag="VXN30_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1356750.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42175..43110
                     /locus_tag="VXN30_000057"
     CDS             42175..43110
                     /locus_tag="VXN30_000057"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1356751.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43122..43508
                     /gene="rplQ"
                     /locus_tag="VXN30_000058"
     CDS             43122..43508
                     /gene="rplQ"
                     /locus_tag="VXN30_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1356752.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            43773..44039
                     /locus_tag="VXN30_000059"
     CDS             43773..44039
                     /locus_tag="VXN30_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1356753.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            44049..45386
                     /locus_tag="VXN30_000060"
     CDS             44049..45386
                     /locus_tag="VXN30_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1356754.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        13676 a         8632 c        10006 g        13114 t
ORIGIN      
        1 aacattgtta gaaatcgatt tgactgtcct gattgattta tcctgttctt atttcatttc
       61 actatagtaa ggtgcagcag gataaagcaa aaccgagaag tttctttctc ggttttgctt
      121 attatgaggt tcctattttc tatgctccaa gagttgattg atgtgatcta cttttttagc
      181 ctctctttta tagagaatag cccagatgat gagatagaca atcgcaaact cgataatgag
      241 ctggaggtag aaaatccagt ggaaggggaa ccaaccagca agagttgcta gtgggacaaa
      301 gccagccagc ataaggaaga aatgagtcag agtggcacga agcatgctcc agtcacggct
      361 gaataagcgg ttgccaaagt tgaagagcat accgatggct gcccagataa gtgtgcagta
      421 gagcaggacc agggcaccgt gaacctgatg ctgatccatc acttggccga tgagagagta
      481 gggatttagt ggtgcgtagg tatttggtgc ataaatgagt gaaaagagga tggagaggat
      541 gaggccgata agaacaccgg cagctgcatc gtgaaatact tgttttttca tagttctaat
      601 ttctccttga tggtttttag ataacggcgt gaagagtagg tgaagctttc gtttttcaag
      661 aaaatttcta ccagaccgtt tggcgtgagc ttgaggtgag agatggaatc gatattgatg
      721 atttctgatt gggaaatttg gataaaattg cttggcaaga gtttaagaac ctgatagagt
      781 cgcaaatcaa tgctgtaggt ctgactcgcg gtttctgcta gaaccttccg attctcgata
      841 tagaagcgct gaatcttgcc aatctcaact agatagacct gatcatcgat ttttcctttg
      901 attttttctc tttggtccag attttctgcg aactcgatga ctttctggac tttttcggtt
      961 tcttgaggtg cttggacaat cagcttttcc tcctcgtaag tctcactaat ctgtagttct
     1021 actttcatag ttttctctcc ttttcagtta tacaaggttg tgatcacttc ctgtatatcc
     1081 ttattaaaca ctattttata gcccatggca aggaaccttg tctatgtgga gggattgggc
     1141 tcctatgtgg tggagctttt ctgttctttc tgaaatatgg tataatagca ctaattaatt
     1201 tctaggaaaa tagatacaga aaggggctga aagatgtctc atattattga attgccagag
     1261 atgctggcaa accaaatcgc agctggagag gtcattgaac gtcctgccag tgtggtcaaa
     1321 gagttggtag aaaatgccat tgacgcgggc tctagtcaga ttatcattga gattgaggaa
     1381 gctggtctca agaaggttca aatcacggat aacggtcatg gaattgccca cgatgaggtg
     1441 gagttggccc tgcgtcgcca tgcgaccagt aagataaaaa atcaagcaga tctctttcgg
     1501 attcggacgc ttggttttcg tggtgaagcc ttgccttcta ttgcgtctgt tagtgtcttg
     1561 actctgttaa cggcggtgga tggtgctagt catggaacca agttagtcgc gcgtgggggt
     1621 gaagttgagg aagtcatccc agcgactagt cctgtgggaa ccaaggtttg tgtggaggat
     1681 ctctttttca acacgcctgc ccgtctcaag tatatgaaga gccagcaagc ggagttgtct
     1741 catatcattg atattgtcaa ccgtctgggc ttggcccatc ctgagatttc ttttagcttg
     1801 attagtgatg gcaaggaaat gacgcggaca gcagggactg gtcaactgcg ccaagcaatc
     1861 gcagggattt acggtttggt cagtgccaag aagatgattg aaattgagaa ctctgaccta
     1921 gatttcgaaa tttcaggttt tgtgtcttta cctgagttga ctcgggctaa ccgcaattat
     1981 atcagcctct tcatcaatgg ccgttatatc aagaacttcc tgctaaatcg tgctattttg
     2041 gatggtttcg gaagcaagct tatggttgga cgttttccac tggctgtcat tcacatccat
     2101 atcgaccctt atctagcgga tgtcaatgtg catccaacta agcaagaggt gcggatttcc
     2161 aaggaaaaag aactgatgac cctggtttca gaagctattg caaatagtct caaggaacaa
     2221 accttgattc cagatgcctt ggaaaatctt gccaaatcga ccgtgcgcaa tcgtgagaag
     2281 gtggagcaaa ctattctccc actcaaagaa aatacgctct actatgagaa aactgagccg
     2341 tcaagaccta gtcaaactga agtagctgat tatcaggtag aattgactga tgaagggcag
     2401 gatttgaccc tgtttgccaa ggaaaccttg gaccgattga ccaagccagc aaaactgcat
     2461 tttgcagaga gaaagcctgc taactacgac cagctagacc atccagagtt agatcttgct
     2521 agcatcgata aggcttatga caaactggag cgagaagaag catccagctt cccagagttg
     2581 gagtttttcg gacaaatgca cgggacttat ctctttgccc aagggcgaga tggactttac
     2641 atcatagatc agcacgctgc tcaggaacgg gtcaagtacg aggagtaccg tgaaagcatt
     2701 ggcaatgttg accagagcca gcagcaactc ctagtgccct atatctttga atttcctgcg
     2761 gatgatgccc tgcgtctcaa ggaaagaatg cctctcttag aggaagtggg cgtctttcta
     2821 gcagagtacg gagaaaatca atttattcta cgtgaacatc ctatttggat ggcagaagaa
     2881 gagattgaat caggcatcta tgagatgtgc gacatgctcc ttttgaccaa ggaagtttct
     2941 atcaagaaat accgagcaga gctggctatc atgatgtctt gcaagcgatc tatcaaggcc
     3001 aatcatcgta ttgatgatca ttcagctaga caactcctct atcagctttc tcaatgtgac
     3061 aatccctata actgtcctca cggacgtcct gttttggtgc attttaccaa gtcggatatg
     3121 gaaaagatgt tccgacgtat tcaggaaaat cacaccagtc tccgtgagtt ggggaaatat
     3181 taaaagtata aaaaagtctg ggaaaaattt tcaaaatcaa aaaaacgcat aaaatcaggt
     3241 gttcaaaaac cttgatttta tgcgttttat catggaaata gttacttcat tttttcctaa
     3301 ttcttttcga aactcttttt aaacgacgtc agttttatca gtaatctcaa aacagtgttt
     3361 tgagctaatt ttgccagttt tgtctgtaac atcgaagttg tgttttacca ctctgcgact
     3421 ggtttcctag tttgctctat gattttcaca gagtattaaa ttgcgatttt gccaagtttc
     3481 tttattcgtc taaaagtaga gtctgttcta tgcgtctaat gtacgaatta ggttgaccat
     3541 ttcaatagct ccttgtgcac actcagaacc cttatttcct gctttagtac cagctcgttc
     3601 tatggcttgt tcaattgtat ctgtcgttag cacaccaaac ataacaggaa tttcgctatt
     3661 taaactgatt tgggcgattc ccttagatac ctcgctacat acataatcat aatgacttgt
     3721 atttcctcta atgacagctc ccaagcagat aattgcatca tattttttac tttttgccat
     3781 ttttgatgca atcagtgata tttcaaaagc tcctggaacc caggctacct cgatatcttt
     3841 ctcgtttaca ttctctcttt tgagattatc tagtgctcca gataataatt ttgaagttat
     3901 aaattcatta aatctcgcta caacaatacc tattttaata ttgtttgcta ctaaattacc
     3961 ttcataagtg ttcatttatt tttcctccat atttaaaatg tgacccattc gatttttctt
     4021 tgtttctaaa taaaaactat cgtaaggatt ggcttctatt tcgattgata ttctactgga
     4081 aatggtaatt ccatattttt ctaactgttc aaccttgtca ggattatttg tcagtaaatg
     4141 aagtgactga agtcccagat ctttaagcat ttgtgctcca atatgatatt ctcttaaatc
     4201 accttcaaag cctaatgcaa gattggcatc aagcgtatcc atgccttgat cttgtaaatg
     4261 ataggctttt aatttattga taagtccaat tcctcgtccc tcctgtcgca agtaaagtaa
     4321 gacacccgaa ccattctcaa caatcatttt catagcttta tcgaattgct gtccacaatc
     4381 gcaacgtaag gagcctaaaa catcccctgt taaacattcg gagtggaccc gacataatac
     4441 attggcttca tcctctatgt ttcccataat aagagcaaga tgatgttccc catttagttt
     4501 atctatatag ctaattgctt tgaaattacc gtatctagta ggcatattga cagttgaaac
     4561 tcgttctacc agctgatcat atacttttct atattcttgt aattctttga tggtaattag
     4621 tggaatgttg tgtttcttcg agaactgaat taaatcatct gttctcatca ttttgccatc
     4681 atgattcatt atttcacaac ataggccaca ctcttttagt ccagctaatt ttaataaatc
     4741 aacagttgct tctgtgtgtc catttctttc tagaacacca cctttttttg caattaaagg
     4801 aaacatgtgt cctggcctgc gaaaatcaga gggtgttata tcttcagcta cacacatacg
     4861 tgcggtcagt cctctttcct cggcagaaat acctgtggtc gtttctttat aatcaattga
     4921 aactgtaaaa gcagtcttat gattatctgt attgttttca accataggtg aaagcattaa
     4981 ttgattagct aaactttcgc tcataggcat acaaattaat cctttggcat aagtagccat
     5041 aaaattaaca ttttctgttg tagctgcttg tgcagaacaa attaagtctc cttcattttc
     5101 tctatccttg tcgtctataa caagaacaag tcgtcccttc tgcaatgctt ctaatgcttc
     5161 ttgtattttt cgatattcca ttgactgatt atcctttctg ctaaaatcca ttttgatata
     5221 atagttcctt agatatttct gattttggag agttatccat cagtttttgc acatatttac
     5281 ctaagatatc attttcaaga tttactgtac tcccgacttg tttactctta agaatggttt
     5341 gttccaaggt atgagggata acagatactg aaaagtttac tttggagact ttagcgacag
     5401 tcagactaat gccgtcaatt gtaatagatc ctttttcaac tattaaatct aaaatttctt
     5461 tttgtgtgtt gatttgatac catacagcat tatcatcttt ttttattgac gagatttttc
     5521 ctgtaccatc aatgtgtcct gtaacgacgt gacccccaag tcgaccgttg acagataagg
     5581 ctctttctag attcacctca cttccatgtt ttaatagagt aagagctgtt cgactccatg
     5641 tttcattcat tacatcaact gtaaaggatt gatgattgaa atgagtaact gtaagacaga
     5701 taccatttac tgctatacta tcgcctaaat ggatatccgt taatattttt gaggctttaa
     5761 ttgatagttt acaattacga gagtctttct gtattctttc aacttttccg atttcttcaa
     5821 ttattcctgt gaacatggat aaatcacttc actttctatg agatagtcat ttcctatttg
     5881 agaaaatgca taaggtttca atctaatagc gccatttggc aaagaaatac cttcacctcc
     5941 gacaggaaac ttggcactac ctccaaaaat ttttggtgca atatatattt tcagctcatc
     6001 aacaatttgt tgttccaaag cactccaatt cattagactg cccccttcta gaactaggct
     6061 atcaatctgc atgtttccta gatgttgcat taaactcgat aagtctatat gattgccttt
     6121 tttctttatg gaaagtattt cacagccatg attttgatat agcttcattt tatttttgtc
     6181 ttcagaggaa gtggcaatgt aagttttaat atcatttgct gtttttacga ttttagaggt
     6241 aagaggagtt cgtaaatgtg tatcgcatat gatacggata ggatttttcc cttcctccaa
     6301 tctacatgtc agcaaaggat cgtcttgaat aacagtattg actcccacca taattgcact
     6361 aacatagtgt cgtaactgat gcacatgctt tcttgcttct tcttcagtaa tccatttgga
     6421 ttgatttgtt ttagtggcta tttttccatc cattgacatt gcatatttca taaaaacata
     6481 gggtacatgc tgggtaatat actttctaaa actttttatt aagttaagac actcattttc
     6541 taaaattcca acagtaactt gaagattatt ttcctcaagt atctttactc cttttccaga
     6601 tacaatagga ttacagtcta ggcttccaat gactactctt gtaataccac tatcgattat
     6661 agcatctata cagggaggtg ttttcccgaa gtgacaacag ggttcaagtg ttacataaag
     6721 cgtcgctccg acaggggatt ttctacagtt tttaagagca tttctctcag catgtgggcc
     6781 accaaaaaac tcatgataac cttgtccgat gatgtgatta tcttttacaa taactgcgcc
     6841 gaccatagga ttgggattga cgtaaccagc ccctttttgt gccagtttta ttgctaattt
     6901 catatatttt gaatcgctca tctcactacc tccaaaaaaa atataccttg aataggggac
     6961 tactcaaggc atacaaaaga aaacttatac gattaacaaa aatactctga aatgacaagt
     7021 aatcatttca gagcacgcaa aaagcacaaa tatactttta tcttctttca tccagactat
     7081 actgtcggct ttggaatttc accaaatcat gcctttcggc tcgtgggcta taccaccggt
     7141 agggaattgc accctgccct gaagatagtt attcaattac agatgattat agtacttaat
     7201 tttgaatatg tcaacagata aataccgatt gtttttgata tactgtattt gtgataatcg
     7261 attctcgctc ctcggataaa gaaaatatga tatactagat aaacgaaata agagagaagg
     7321 aatactatgt acgcatattt aaaaggaatc attaccaaaa ttactgccaa atacattgtt
     7381 cttgaaacca atggtattgg ttatatcctg catgtggcca atccttatgc ctattcaggt
     7441 caggttaatc aggaggctca gatttatgtg catcaggttg tgcgtgagga cgcccatttg
     7501 ctttatggat ttcgctcaga ggatgagaaa aagctctttc ttagtctgat ttcggtctct
     7561 gggattggtc ctgtatcagc tcttgctatt atcgctgctg atgacaatgc tggcttggtt
     7621 caagccattg aaaccaagaa catcacctac ttgaccaagt tccctaaaat tggcaagaaa
     7681 acagcccagc agatggtgct ggacttggaa ggcaaggtag tagttgcagg agatggcctt
     7741 cctgccaagg tcgcagtgca agcaagtgtt gaaaaccaag aattggaaga agctatggaa
     7801 gccatgttgg ctctgggcta caaggcaaca gagctcaaga aaatcaagaa attctttgaa
     7861 ggaacgacag atacagctga gaactatatc aagtcggccc ttaaaatgtt ggtcaaatag
     7921 gagcagagaa tgacaaaacg ttgttcgtgg gtcaagatga ccaacccact ctacatcgcc
     7981 tatcatgatg aggagtgggg ccagcccctc catgatgacc aagcgttgtt tgagttgttg
     8041 tgtatggaaa cctatcaggc aggcctgtct tgggaaacgg tactcaacaa acgccaagct
     8101 ttccgagaag cctttcatag ctatcaaatt cactcagtcg cagagatgac tgacactgaa
     8161 ttggaagcca tgctggagaa tccagctatc attcgaaata gagccaagat ttttgctaca
     8221 cgcgctaacg cccaagcttt tctacagtta caggcagagt acggctcttt tgatgcctat
     8281 ctttggtctt ttgttgaggg gaaaactgtc gttaacgatg ttcctgatta tcgccaagcc
     8341 ccagttaaaa cacccttatc tgagaaatta gccaaagatc tcaaaaaacg aggcttcaag
     8401 ttcacaggcc cagtcgccgt attgtctttt ctacaggctg cagggctagt tgatgaccac
     8461 gagaatgatt gtgagtggaa aggtcttaaa tgatgtctaa caaaaataag gaaattctga
     8521 tttttgcgat tctctataca gtcctctttg tgtttgatgg cgttaaattg ctggcttctt
     8581 taatgccatc taccattgca aattatcttg tttatgtagt tttagcttta tatggctcct
     8641 tcttgttcaa ggatagattg atccaataat ggaaggagat tagaaagact aaaagaaaat
     8701 tcttctttgg agtcttaaca ggatggctct ttctcattct gatgactgtt gtctttgaat
     8761 ttgtatcaga gatgttgaag cagtttgtgg gactagatgg acaaggtcta aatcagtcta
     8821 atattcaaag tacctttcaa gaacaaccac tactgatagc tgtttttgct tgtgtcattg
     8881 gacctctgat agaagaatta tttttccgtc aggtcttatt gcattacttg caggaacggt
     8941 tgccaggttt actaagcatt attctggtag gacttgtttt tgctctgact catatgcaca
     9001 gtttggctct atcagagtgg attggtgcag ttggttactt aggtggaggc cttgcctttt
     9061 ctattattta tgtgaaagaa aaagaaaata tctactatcc cctacttgtt cacatgttaa
     9121 gcaacagcct ctccttaatc attttagtta tcagtatagt aaaatgaaat aagaacagga
     9181 cagtcaaatc gatttctaac aatgctttag aagtagaggt gtactattct agtttcaata
     9241 cactgtaaca tgtgatgaaa atgccagtaa tgataccgag aaaaaagctg agaaactttt
     9301 cccagcttta tttgttatag tcaaagcgaa tgacttgttc ctgtgcatct acatgagcat
     9361 ggaccccaaa gggtacaatt gctcttggag ttgcgtggcc gacattcaga ttatagacaa
     9421 tcgggatatt gttgtcaatg atatccaata gtgcctcttt atagtcgtca tggaaaattt
     9481 catccatagg ttttccgacc aagagtccat tgatgaccgc gaatatgcca gtgtccttta
     9541 aagttagcaa catctttttg aagtcttctg gcttaggctc ttcttcgctt gtttcgagca
     9601 agaggatttt cccttcccag tctgacaagt cagggaaaag tttgtatttt tggcagagtt
     9661 ccgtgctatc tgcgtatcga gagttgtcaa agatatcgta gagggattcg aggcaaccac
     9721 cgaggatttt cccctcgaac tgggcacttc cttgcaacaa gtcaaaacct gtatttgtat
     9781 gactgacacg aggtgttccc agggccgtgg gactaaaatc agttcgttcc tcataccaaa
     9841 cgtcactagg gcggatttct gaaattcttc ccgtctcaat caattcttta aagtagtgaa
     9901 ggctataggc tagcatttct ttgtctaatt cacaaatgtc tgctaaaaag gattgaccat
     9961 aaaaagtctt gattcctaat ttatgcaaca tgaggtggtt catggttgta tccgagaagc
    10021 caagaaaaat tttttgtttg ataacctttt ggagttggtc attttcaaaa agataaggta
    10081 gcaagcgata ggtatcgtct ccaccgatgg cacataggat catgtcgatg ctatcatcag
    10141 aaaaggcatg aatcaaatcc tctgcacgag cttcaggatg gtccttgata aagtctaatc
    10201 cttttaacga atggggcaaa aagatgggat tgagtcccag atccttgaga cgttggatac
    10261 ccaagtccac ttcgtgtttg acaaaatcct ctccgataat gccactagat aaactaacaa
    10321 taccaatagt agaaaccata tctcatcctc ctagaaatag attgagccta ttttatcaca
    10381 aaatataaga gaaagctatg tgggatttca gaagaatact tttaatactc ttcgaaaatc
    10441 tcttcaaacc acgtcagttt tatctgcaac ctcaaagctg tgctttgagc aacctgcagc
    10501 tagcttccta gtttgctctt tgattttcat tgagtataac tcaaaaaagt aagtgaaaaa
    10561 gcaaccgcac ctgcagttgc ttttatcgtt ttcttattct tgatagcgtg ttggtcctgc
    10621 acttgcacta cggatattca agcgtttctt gagatagaag cgaagggcta ggagggctgc
    10681 tccaataata gctagtggca atggatccag tactgggtta aggctagctg gtaggaagct
    10741 tgttgcaaag aagacaagca accaaaggaa catagaagct aggataacta gtacagattt
    10801 ccagaaaggt ggacgttgac tgcgatccat atctggtcca tagtattggt aaacaaagta
    10861 gtacatcaag tagaaggcaa atccaccaac cagtccaact aatagaagag taatcaatcc
    10921 atagccgaaa gcttgatctg ccgcaaagaa ggttgtgagg gcgctgacaa gggcaaagag
    10981 gctagtgatg aaaagagctg agtccataat catcagtttt ggatcatcat tttcttttgg
    11041 atgctctttt tcgtactgct ctttgacagt gaagctatga gcccaatgag ttggtgcgcc
    11101 gtatagggaa cgggcagttg tacctttaga ttgctcctca aggatgtggg gaatgacttc
    11161 ctcaaaaata gtctggattt cagcgtctgt tttcccatct ttgatgaatt gttgggtagc
    11221 aatgtggaca aactcttggt ttttcttagt taatttttgt agatcaattt gagacatagg
    11281 aactcctctt agaaccattt tttatggatg agatagagag tgagcgagac actcatagca
    11341 aaggcgataa agacgattaa ccagaaggca tttggctctc cgtttagggg gatttcatta
    11401 tccttaaagt tcatcccgta ggcagaaaag accatggttg ggatggacat gacgatggtc
    11461 acaagggcca aggttttcat gatgttgttc tggttgttag aaatgataga ggcaaaggtc
    11521 tctgtcatag aatgcaagac gtttccataa atatctgcca tctcgatggc ctgttgggtt
    11581 tcaatcaggg tgtcttcaag caggtcttcg tcctcaaggt atttcttgat attgctggtt
    11641 gaactggtca atttcttaat cacgcgctca tttgttttga gggaggcctt gaaatagacg
    11701 atagtttttt ccaattccat gagctcaatc aattcttcat tacgagttga ttgatgcagt
    11761 tgactttcga tttgttcact cttgcggtcg attgaacgaa gggctgttag gtaaagctct
    11821 gcattgcgat aaagaatttg aaagataaaa cgtgaacgca tgaaggtata gaaattacgc
    11881 aatcgacggt tgataaagac atcaaggaca ggtagtggtt ccaaacacgt agtgataatg
    11941 gtttcctcag tgatgataat accaagcggg atggttacgt agtaggtgcg gttatttctt
    12001 tcctccgtga ccggcacgtc tacgataatc agggtatact cgtcttcaat ggtaatacga
    12061 gacatttctt ccgcatcgag cggtgctcga aggtcggcaa tatcaatatc gaaggtgttg
    12121 gcgatttcga gtgattcatt ttgagtcgga ttgacgagat tgatccaagt acccggttca
    12181 agcgtatcga tctctttaaa ttcagttgtt gtagagagaa aaacttgttt catatccctt
    12241 atcctttctc atttttcaga ttttttcaca ctgtactatt atactacaaa atcggccttt
    12301 tgggtaatag aatctcactt tttttggtat aatggtaagc aataatggac tagaaagaac
    12361 aaagatgcaa gataaaattg tcattcatgg ggcgcgtgcc cataatttaa aaaatattga
    12421 tgtggagatt ccgcgagaca agttggttgt cgtgactggc ttgtcaggtt cagggaagtc
    12481 cagtctggct tttgataccc tctatgcgga gggacaacgt cgctatgtag agagtttgtc
    12541 cgcctacgct cgtcagttct tgggaaatat ggaaaaacca gatgtagatg ccattgatgg
    12601 tctcagccca gctatttcca tcgaccagaa aacgactagt aaaaatcctc gctcgacggt
    12661 gggaacgacg actgaaatca atgactatct gcgtctcctc tacgcacgtg tggggacgcc
    12721 ttactgtatc aacggacatg gagctatcaa tgcttcttct gtggagcaaa tcgttgataa
    12781 ggttttggag ttacctgaac gccagcgctt gcagatcttg gctcctgtca tccgcaagaa
    12841 aaaaggccaa cataagaatg ttatcgagaa ggttcagaaa gacggatatg ttcgtgtccg
    12901 tgtggatggg gaagtctatg atgtgaccga agtgccagag ttgtctaaga gcaagcaaca
    12961 caatatcgat gtcgtggttg accgtattgt tatcaaggag ggcattcgta gccgtctctt
    13021 tgattccatt gaagctgccc ttcgtatcgc agaaggttat gtgattatcg acaccatgga
    13081 cgattcggag ttgctcttct ctgagcatta tgcctgtcca gtttgtggat ttactgtccc
    13141 agagttagag cctcgtctct tctctttcaa tgctcccttt ggctcttgta gtgagtgtga
    13201 cggcttgggg attaagctgg aggtggatac tgatttggta gtgccagatg ccagcaaaac
    13261 gctacgtgag ggggctttgg caccttggaa tcctatctca tctaactact atccaaatat
    13321 gctagagcag gctatgacag cctttggagt ggatatggat aagccctttg aggacttgtc
    13381 agaagaagat aagaacttga ttctctacgg ctcggatggc aaagaatttc attttcacta
    13441 tgagaatgaa tttggcggtg tgcgcgatat tgacattcct tttgagggag tcgtcactaa
    13501 tatcaagcgt cgttaccatg agactaacag tgattacacg cgcactcaga tgcgtctcta
    13561 catgaatgag ctgacctgcg gaacctgtca aggctatcgt ctcaatgacc aggccttgtc
    13621 tgtccgtgtg ggcggccagc aagggccaca tattggagaa atctcagacc tgtctatcgc
    13681 tgaccacttg gacttggtga gccagttaac tttgtctgaa aatgaagcca tcattgctcg
    13741 tcccattctc aaggaaatca aggatcgttt gaccttcctt aataatgtgg gtcttaacta
    13801 tctaaccctg tcacgttcag caggaaccct ttcaggtggg gaaagtcagc gcattcgttt
    13861 ggcaacccag attggttcca acctatcagg tgtcctctat atcctagacg agccgtcaat
    13921 cggtcttcac cagagggaca atgatcgcct gattgccagt ctgaaaaaga tgcgtgactt
    13981 gggcaatact cttatcgtgg tggaacacga cgaagatacc atgcgtgagg ctgattatct
    14041 gattgacgtt ggtcctggtg ccggtgtttt tggtggtgag attgttgcag caggtacacc
    14101 taagcaagtg gctcgcaaca gcaagtctat cacaggtcag tacttgtcag gcaaacgtgt
    14161 catcccagta ccagaagagc gccgtgtcgg aaatggtcgt tttattgaag tgataggagc
    14221 gcgtgagaac aacttgcaaa atgtcacggc tcgctttcca ctaggaaaat ttatcgcagt
    14281 gacaggtgtg tcgggttcag ggaaatcgac tctaatcaac agcattctca aaaaagccat
    14341 tgcccagaag ctcaaccgta attcagacaa acctggtaag tttaagacta ttacagggat
    14401 tgagcatgtc gaccgtttga ttgatattga ccagagccct atcggacgga cgccgaggtc
    14461 taacccagct acctatacag gagtttttga cgatatacgt gacctttttg ctcagacaaa
    14521 tgaagccaag attcgaggct acaagaaggg gcgcttcagt ttcaacgtca agggaggtcg
    14581 ctgtgaagct tgctcaggtg acgggattat caagattgag atgcacttct tgccagatgt
    14641 ttatgtggct tgtgaagttt gccacggaac ccgctataac agtgaaaccc tagaagttca
    14701 ctacaaggaa aagaatattt cgcaggtctt ggatatgacg gtcaacgatg cggtagaatt
    14761 tttccaacac attccgaaaa ttcaacgcaa actccagacc atcaaggatg tagggctagg
    14821 ctatgtgacc ttgggacagc cagctaccac tctttctggg ggagaagccc aacgtatgaa
    14881 gttagctagc gaactccaca aacgctcgac aggaaaatct ttctacattc tggatgagcc
    14941 gacgacaggg cttcatacag aggacattgc tcgcctgctc aaggttttag ctcgctttgt
    15001 agacgatggc aatacagtcc tcgtcatcga gcacaatctg gatgttatca agacggcaga
    15061 ccatatcatt gacttgggac ctgagggcgg tgtcggtggt ggaaccatca tcgtaacagg
    15121 aactccagaa gaagtagctg ccaacgaagc cagctataca ggacactatt tgaaaggaaa
    15181 gttacatcat gaataaacgc gtacaagcat ttctagctaa aatgcaagaa aaagaactag
    15241 atggtatcat catcaacaat cttaaaaacg tctattattt gactggtttt tggggctcaa
    15301 acggaacagt ctttatcagc cgtgaccgtc aggtcttagt gacagactct cgctatatca
    15361 tcgcagctaa gcaagaaacc agtggttttg agattgtggc tgatcgtgat gaattggctg
    15421 tcattgcagg aattgttaag gacatgggct tgactcgtat cggttttgaa gatgagattt
    15481 cagtgtctta ttaccaccgt atgcaggcag cttttgcagg tttgaacttg cttccacaaa
    15541 ctcagtttgt ggaaggtctt cgtatgatta aggatgaggc agagattgca gcgattcgca
    15601 aggcttgttc tatctcagac caagctttcc gcgatgcgct tgactttatc aaaccaggaa
    15661 aaactgaaat tgagattgcc aacttccttg atttccgcat gcgtgagttg ggagcatctg
    15721 gcttatcttt tgatacgatc ctagctagcg gtatcaattc ttctaaaccc catgcccatc
    15781 caatgcacaa accagtggag ttgggagaag ccattaccat ggacttcggc tgtctctatg
    15841 accactatgt cagtgatatg acccggacta tctatctagg gcatgttagc gatgagcagg
    15901 cagagattta caatacggtt ctaaaagcta accaagcctt gattgaccaa gctaaggcag
    15961 gcttaggttt ccgtgacttt gacaaaatcc ctcgtgatat tatcattgag gcaggttatg
    16021 gtgactactt tactcacggc attggccacg gtattggtct ggatatccat gaggaaccct
    16081 actttagtca gacttctaca gaaactatta agacaggtat ggccttgacc gatgaaccag
    16141 gtatctatat cgaaggcaaa tatggcgttc gtatcgagga tgatatcctg attacagaga
    16201 caggttgtga attattgacc ctagctccaa aagagttgat agtcatttag ttgtttgtca
    16261 agaaaaaagt acgaaaatga cgaaaaaagt caaaaaaaat taaaaatagg tcgcaagtcg
    16321 gatgtttttt atggtataat agactaaact gatagtaaca tgtagcgaaa ggggtaggta
    16381 catgattaaa atttatacag tctcaagttg tactagctgt aaaaaagcaa aaacctggct
    16441 caatgcccac cagttaagtt ataaagaaca aaaccttggt aaagaaggaa ttacgagaga
    16501 agaattactg gatattctaa ccaaaacaga taacggaata gccagcatcg tttcgtctaa
    16561 aaatcgctat gccaaagccc ttggagtgga tattgaagat ttgagtgtca atgaagttct
    16621 caatctgatt atggaaacac cgagaatttt aaagagccca atccttgtag atgaaaaacg
    16681 cctgcaagtt ggctacaagg aagacgatat tcgtgccttc ctaccacgct ctgtccgtaa
    16741 tgtagaaaat gcagaagcac gtttgcgtgc agctctataa acatcaaggc tgggagcact
    16801 tcccagtctt attctatttt aatttcaaaa agaaagaaga aagaaatgaa aaaaatagtt
    16861 cttgttagtc tagctttcct ttttgtcctg gttggttgcg gacagaaaaa agaaactgga
    16921 ccagctacaa aaacagaaaa agatacgctt cagtcggcat tgccagttat tgaaaatgct
    16981 gagaagaata cagttgtaac caagactttg gtcttgccca agtcagatga tggtagccag
    17041 cagacacaaa caattactta caaagacaag acttttttga gtctaactat ccaacaaaaa
    17101 cgtccagtct ctgatgagtt gaagacttat attgaccaac atggagtgga ggaaactcaa
    17161 aaagctcttc ttgaagcgga ggagaaggat aagtctatca ttgaagctcg taaattggca
    17221 ggtttcaaac ttgaaacaaa actattgagc gcaacggaac ttcaaacaac gactagtttt
    17281 gattttcaag ttctggatgt caagaaggct tcccagttgg aacatctgaa gaatattggt
    17341 ttggaaaatc ttttgaaaaa tgaaccaagc aaatatattt cagatagatt ggcaaatggc
    17401 gcgacagaac aatagaaaat ccaaataaat agactgcctg aattgtagaa ggtaaggaat
    17461 tatgctataa tggtactatt ctaaggaaag agggtgttag aatgggattt actgaagaaa
    17521 cagtacgttt taaattggac gattccaata aaaaagaaat tagcgaaact ttgacagatg
    17581 tttatgcttc gttgaacgat aagggttaca acccaattaa ccaaatcgta ggttacgtat
    17641 tgagtggaga ccctgcctac gttcctcgtt ataataatgc acgaaatcaa atccgtaagt
    17701 atgagcgtga tgaaatcgtt gaggaattgg ttcgctacta cctcaaagga caaggagtcg
    17761 atctataacc tatgagaatt atgggattgg acgtcggttc aaaaacggta ggggtggcga
    17821 ttagcgatcc gcttggtttt acagctcaag ggcttgaaat catccagata aatgaagaac
    17881 aaggccaatt tggttttgac cgcgttaagg aattggttga tacttacaag gtggaacgat
    17941 ttgtagtggg cttgcctaaa aacatgaaca atacaagtgg accgcgcgta gaagctagtc
    18001 aagcctacgg agcaaagcta gaagagtttt ttggtttacc agtagactat caggatgaac
    18061 gcttgacaac agtggctgct gagcgcatgt tgattgaaca agcagatatc agtcgcaata
    18121 agcgcaagaa agtcattgat aagttagcag ctcagctgat tttacaaaat tatttagata
    18181 gaaaatttta atataaagga gaggctatgt cacacgatca taaccacgac cacgaagaac
    18241 gtgaactaat cacactagta gatgagcaag gaaatgaaac cttgtttgaa atccttttga
    18301 caattgatgg aaaagaagaa tttggtaaaa actatgttct tctagtacca gttaacgcag
    18361 aagaagacga agacggacaa gttgaaatcc aagcttactc attcatcgaa aacgaagatg
    18421 gaacagaagg cgaattgcaa ccaatcccag aagactcaga agacgaatgg aacatgattg
    18481 aagaagtctt caacagcttt atggaggagt gatacagtct gggagactgt atcagcccaa
    18541 ctcccagaaa tggggaagag ttggaacatc cagtggatgt ttttagccca gttcctttaa
    18601 ataagagaga actggtaagt ccgggggacg tttttagcct gacgctaaaa ataaaaacag
    18661 tcagtaagtt cgggggatgt tttagcccag ctccttgaaa taagatagag ctggtaagtc
    18721 tgagaaactg tatcagccca actcccagaa atggggaaga gttggaacgt ccagtgaaca
    18781 tttttacccc aactcttaga aattgagaag agttggaaca tctagtggat gtttttagcc
    18841 catattcaca ttcatagtag ctagttatta gctaaccagg taagaggtcg ggacgaaaat
    18901 cctggccttg tttttattag taaccatact ttaatgcgat aattgattgg acttttgtca
    18961 aggagatgtg tatgtttgaa gtagaagaat ggctccatag tcggattggt ttgaattttc
    19021 gatcaggttt gggccgaata cagcaagcgg tggatttgtt aggaaatcct gagcagtctt
    19081 accctattat ccacgtaaca gggactaatg gaaaaggatc taccattgct tttatgaggg
    19141 aattatttat ggggcatggc aaaaaagttg cgacctttac ctcccctcat atcgtctcta
    19201 tcaatgaccg aatctgcatt aatgggcaac ctatagcaga cgcagacttt atccgtttgg
    19261 ctgatcaggt caaggagatg gagaaaacgc ttctgcaaac tcctgaccag ttgtcctttt
    19321 ttgaattgct gaccttagtt gcttttcttt attttaggga gcaagaggtg gatttggttt
    19381 tattagaagt gggaattggt ggcttacttg acacgaccaa tgtggtaact ggagagcttg
    19441 ctgtcatcac ctccattggg cttgaccatc aagaaacctt gggtgatagt ctagaagcaa
    19501 ttgcagagca gaaagctggt attttcaagg ctggtaagaa ggcagtgatt gcgaaattgc
    19561 ctccagaagc taggcttgtc tgtcagaaaa aagccgaatc tttagctgtt aacctttatc
    19621 aggcaggtca agatttttta atgctgaatg gtgatttttc aagctcttta ctaaatcttt
    19681 cacagctgaa cataggctta gaaggagtct atcagcagga gaatgcagcc ttggcgttgc
    19741 aaacttttct tctttttatg ggagaaggaa aggaagctgt tgatgaacag gctgtaagaa
    19801 aggccttgga acagacccat tgggctggtc gcttggagcg tattcgccca cagatttact
    19861 tggatggtgc tcataacctc cctgccttga ctcgcttggt tgagtttatc aaagaaaaag
    19921 agcaggaagg ttatcgacct caaatcctct ttggatcctt gaaacgtaag gattatcaag
    19981 ggatgttggg ttatctgact gaaaatttac ctcaagtgga actcaaaatg actggctttg
    20041 actatcaagg gtctttggac gagacagatg taacaggtta ccatgtaatt ccctcttacc
    20101 gagaatttat cagcattttc gaagaaaggg cagacgctca agacttgctg ttcgttacag
    20161 ggtctctcta ttttatctca gaagtacggg gctacctgct ggaccgtgag cagataaatt
    20221 gacccccttg tttgaacttg ttatactagg ggaattgctg gttagagaaa atttctctaa
    20281 attggtagca gaaaggaaat tcatcatgaa attaaaaaga ttcacacttt ctcttgcttc
    20341 tctagcaagt tttagtctct tagtagcttg ttcacaaaga gctcaacagg ttcaacagcc
    20401 tgctcaacaa cctgctcaac agaataccaa tactgcaaat gcaggaggta accaaaatca
    20461 agcggctcca gtacaaaacc aacctgttgc tcaaccgacc gatattgatg ggacttatac
    20521 tggtcaggat gacggagacc gtatcacttt agtggtaact ggaacgactg gtacatggac
    20581 tgagctcgaa tctgacgggg atcagaaagt caaacaggtt acatttgatt cagcaaatca
    20641 acgcatgatt attggcgatg atgtcaaaat ttacactgta aacggtaatc aaatcgtcgt
    20701 agatgatatg gatagagacc catcggacca aatcgtttta actaaataag actatgcaag
    20761 tagaaagggc tggatttttc agccttttta cttttaccta gaaaataatc ataaatactt
    20821 gccttctacc gaatacctgc gttatactag tatcaattac ttgtttttag cattcaaact
    20881 atcaaggagg ggatatgaaa tataggaaat ttcaattatt gatgtccaag tatggcttta
    20941 gtctttcgat tatgctactt gaactttgtc ttgtttttgg tctctttctt tatttaggac
    21001 gcatggctcc cattttatgg attactgtcc tcattctact gagtatcatc acaatcattt
    21061 cgatagtcaa ccgtaatacg actcctgaga ataaggtaac ctggttgtta gtagcctttg
    21121 tgccagtatt tggtcccttg ctctatctga tgtttggtga aaggcgattg tccaaaaaag
    21181 aaatcaaaca actgaagaag ctaggctcta tgcatttcca agaagcaaat agccagctac
    21241 taaaagagaa attaaaagaa agtgacaagg cagcttatgg agtcatcaag tccttattga
    21301 gtatggatac caatgctgac atctatgatc aaactgcctc tacatttttt cctaacggag
    21361 aagctatgtg gaaaaagatg gtagaagatc ttaaaaaggc tgagaaattt attttcttgg
    21421 aatattacat tatagaagaa ggtttgatgt ggaatcgcat actagatata ctagagcaaa
    21481 aggtagctca gggtgtagag gttaagatgc tctatgatga tatcggctgt atggctactt
    21541 taacaggaga ttatgcacat cgacttcgtc agctgggcat cgaggcccat aaattcaata
    21601 aagttattcc tcgtctgaca gtggcttata ataacagaga tcatagaaaa atattgattg
    21661 ttgatggtca gatagcctat actggtgggg tcaatctggc agatgagtac attaaccacg
    21721 tcgagagatt tggttattgg aaggatagtg gaattcgctt agacggacta gcagtaaaag
    21781 ccctgacacg cttatttttg accacttggt acattaatcg aggagaaatt agtgattttg
    21841 atcaatatca tttagaaaat cattctatcc cgagtgacgg tttaaccatt ccatacggaa
    21901 gtggacccaa gccaattttt cgagcgcagg tagggaaaaa agtttatcag agtttaatca
    21961 atcaagcaac agaatcggtc tatattacga caccttattt gattatagat tatgatttaa
    22021 cagagacaat caaaaatgca gctatgagag gggtcgatgt tcgaattatc accccttaca
    22081 taccagataa gaagttcatt cagttagtca cgagaggagc ttatcccgac cttctttctg
    22141 ctggtgttcg gatttatgag tatagtccag gttttattca tagtaagcag atgttggtag
    22201 acgaagattt tgcggtggtg gggacaatca atctcgacta ccggagcttg gtacaccatt
    22261 atgaaaatgc agtcttactc tataaaactc cttctataag ggaaatcgcc cgagattttc
    22321 gaaatatatt tgcagattct caggaagtct atcctcattc tatcaaaacg agctggtatc
    22381 aaaagcttgt aaaagaaatc gcccagctat tcgcccctat cttataagaa atctagaact
    22441 taggatacaa accagaccta tttttcttgt cttttacgaa tagaaagaaa gaggagggga
    22501 attcatgcta actaaggaag aagtgaatga tatgattgag ttcaaacaga cacatttaca
    22561 ccgctttcgg gaaattgaaa cctttgtgaa actatgcaaa aaatcactca aacagccatc
    22621 gcaagctgac aatcctagga ctttttgata tactaagaca gataaattga acaggagaaa
    22681 cgatatgagt gtgtatggta gagtagaaga agttcataag gagaatcgtg aacctctaga
    22741 ataccaaatc gaacaagaat cgcatcatcg tgaatcaagt cgtcttcctt tggtgaaaat
    22801 tttactatgg agtacgcttg taacggggat aactctagga gtaccgctat tactcgactt
    22861 aatgagtgca caagaagtgc aggattttta tgcaggttgg gcgcttcatc agacagggaa
    22921 gatttacagc gactattatg gaagtcaagg tttgctttat tatttgctga cttacgtgag
    22981 tcagggcgga tttttctttg ccatctttga gtggttagcc ttggtagcag gaggattttt
    23041 cctttttaga tcagcggaca ccttgacaga gcaaggagac caagctggac atctggtgac
    23101 tattttttac atgctagtta caggtcttgc ttttggtgga ggctatgcga ctcttttagc
    23161 gcttcctttc ttattcgcag cctttagttt agttgcggct tacctaagca atccaagcca
    23221 tgataaggga tttgtacgga ttgggctagc tttggcaggc ggatttttct ttgctccctt
    23281 atcatcgctc ctgtttattg ctgtagtgag tttaggcttg ttggtcttta accttgggca
    23341 tagacgcttt gcgcatgggt tttatcagtt tcttgcagtg gctttaggtt tttcacttgt
    23401 cttttatcca actgcctact atagtgctgc aacaggaagt tttggggatg cgattagtgg
    23461 tattcgttat cctattgaca gtattcgctt tgattttact tctaaaattt tagagaatat
    23521 gtttttttat ggcttgttgt cccttggttt gggatttgtg gtttgtatct ttttaggtct
    23581 ctttcaatcc aaacccttta aactatacgt catttcggtt cctgcaagtc ttgtggtaat
    23641 tttaggtttg attttacttt tcttttcaca agagcctctg cacgcttctt atttgatggt
    23701 cgtcttccct gttttcctac ttttattggt aaccaatatt aagagtcaac agagggggcg
    23761 tagtgctaga agaagcctaa gagaaacgcc agttagccta tggagtcgtt tcttcaaagg
    23821 aaatctatat ctgctagttt ttgggtttgt ctatcttttg tctgttcctt ttttgatgaa
    23881 gtttgtcctt tatccagtac cttatcaaga acgtaatcgt cttgctgatt tggtaaaaga
    23941 ggagacaaat acggaagatg ctatctatgc atgggatgat actgcgactc tttatcgtaa
    24001 gagtgagcgc ttgtcgccat cggcgatttt gtccccgttg cactatacag caactgagga
    24061 aaatcgtaat aagttactta atgacttgaa agaaaaacaa cctaaggtga ttgtggtaaa
    24121 tgacaaggtg gtagtctggt ctgaagtgga aacactttta aaagaaaatt accaacaagt
    24181 aaagactgat tactcagagt ttaaagtcta taaaattaaa taaccaaatc aatatcttgt
    24241 gtatttttaa aaattttagg atttttaaca caagatattg atttttcttt ttagagtggt
    24301 ataatacttt ttagaaagaa cattttagaa aagagcatgc gtatgattgc actagaagaa
    24361 aaaattacaa ttttgccaac tctcttcgtc gagaaacgag atgggagacg tgttgtattt
    24421 gatgtggaca agattgacaa ggctctccac aaggcggctg acaaggttat ggatgtgaca
    24481 cccctggttg aaaaacgcct caatgatctg actgagcgaa ttattacaga aattcatagt
    24541 cgctttccac agggaattaa gatttacgaa attcaaaata tcgtagaaca tgaactcctt
    24601 gaagccaaag aatatgcgct ggctgaggag tatattactt atcggacaca gagggatttt
    24661 gagcgctcaa aagcgacgga tatcaacttt agtattcata aacttctcaa caaagaccag
    24721 acagttgtca atgaaaatgc taataaagac agtgatgtct ttaacactca gcgtgatttg
    24781 acagcaggga ttgttgggaa atcaatcgga ctgcaaatgc ttcctaagca cgtagccaat
    24841 gcccaccaaa agggggatat ccactatcac gatttggact acagtcccta tacccctatg
    24901 accaactgct gtttgattga ttttaagggt atgttggaaa atggttttaa gattggaaat
    24961 gcagaggtag agagtcccaa gtctatccag actgcgacag cacagatttc tcaaatcatt
    25021 gccaacgttg cttctagcca gtacggtggc tgttcagctg accgtatcga tgaaattttg
    25081 gcgccttatg cagagaagaa ctatcaaaaa catctcaaag atgcagaaga gtgggtattg
    25141 cctgaaaaac gggaagatta cgcttggaag aaaacgcaaa aggacatcta cgatgccatg
    25201 caatctcttg agtatgaaat caatactctc ttcacttcaa atggacaaac accttttact
    25261 tcgttaggtt ttggtctggg aaccagtcgt tttgaacgag aaattcaaaa agctatttta
    25321 aacattcgca tcaagggtct tggttcagaa caccgtacgg ctatctttcc taaacttatc
    25381 tttacgctta aaagaggcct caacttagag gaaggaactc ccaactatga catcaagcag
    25441 ttggctctag agtgtgcaac caagcggatg tatccagacg tcttgtctta tgataagatt
    25501 gttgatttga caggttcttt caaagttcct atgggctgcc gttctttcct tcaagggtgg
    25561 aaggatgaaa atggtgtaga agtcaattca ggtcgcatga atctgggtgt tgtgacggtt
    25621 aatctgcctc gtattgctct tgagtctgaa ggtgatatga ataagttctg ggaaatcttc
    25681 aacgagcgaa tgaatatcgc agaagatgct cttgtttacc gtgtcgaacg cactaaagag
    25741 gcgacaccag cgaatgctcc tattctttat cagtacggtg cttttggcca tcgtctaggt
    25801 aaagaagaaa gtgttgacca gctctttaag aatcgtcgtg cgaccgtttc gctgggctat
    25861 atcggcttgt atgaagtagc gacagttttc tttggtaaca gctgggaaag taatccagat
    25921 gctaaggaat tcacgctaga catcattcac gatatgaaac gccgtgtaga agagtggtca
    25981 gaccaatatg gctaccattt ctctatctac tcaacaccat ccgaaagtct gacagaccgt
    26041 ttctgccgac tagatataga caagtttggc tctattcctg atatcacaga caaggaatac
    26101 tacaccaact ctttccacta cgatgttcgt aaaaatccaa caccgtttga aaaattggac
    26161 tttgagaaag tctatccaga agcaggtgcg tcaggtggtt tcatccatta ttgtgagtat
    26221 ccagtccttc agcaaaatcc aaaggccttg gaagctgtct gggattatgc ttatgaccgt
    26281 gtaggctatc taggcaccaa tactccgatt gaccgttgct acaagtgtga ctttgaaggg
    26341 gattttgaac caactgagag agggtttgct tgtccaaact gtggcaatag cgatcctaaa
    26401 acagtagacg ttgttaaacg gacttgtggc tatcttggca atcctcaagc gagaccaatg
    26461 gttaacgggc gtcacaagga aatcgctgcg cgtgtcaaac atatgaatgg ttcaacgatt
    26521 aaaatagctg ggcatcaagt aacaaattag aaagaaatga aatgggaaaa tatcaactag
    26581 acgataaggg gcgcgcacaa gtgacccgtt atcacgagaa gcactctaaa ggtggagctg
    26641 gtaagaaaga acgcttgctt agcttcagag aacaattttt aaacaagaac aagaaaaaat
    26701 aaaagtgaga gccagctctc gcttttctca tagtgggagg tagggatgga attacgcaga
    26761 ccaagattag cggataagaa agctgtttta gatatgatga cagagtttga aaagagccaa
    26821 tcagcccatg atggaggatt ttgggataca gagaattttg tgtatgaaga gtggttggaa
    26881 agcaatcagg aacaggaaat ggggattaat ctgcctgaag gatgggttcc tgcaattcag
    26941 ttagtggctt tttctgagaa aggtcaagca gttggatttc ttaatctccg gttgcgcctc
    27001 agtaactttt tactagaaga aggtgggcac attggctact ccatccgtcc atctgaaaga
    27061 ggcaagggtt atgcaaaaga gactctccgt cagggcttgc aagttgctaa ggaaaagaac
    27121 atcaagaaag ctctggtgac ctgtagcgtg aataatcctg ctagcagagc agtcattcta
    27181 gcaaatggtg gaatatttga ggatgctcac aatggagtcg agcgttattg gatagaggta
    27241 gcgaatgaat aatccaaaac cacaagaatg gaaaagcgag gaacttagtc aaggtcgtat
    27301 cattgactac aaggccttta actttgtgga cggcgaaggc gtgcgtaact ctctctatgt
    27361 atcaggctgt atgtttcact gcgagggatg ttataatgtt gcgacttggt cttttaatgc
    27421 tggcattccc tatacagcag aattagaaga gcagatcatg gcagatcttg ctcagcccta
    27481 tgttcaaggt ttgactttgt tgggagggga gccttttctt aatactggga ttctcttgcc
    27541 acttgttaag cggattcgga aggaattgcc agacaaggac atctggtcct ggaccggcta
    27601 cacttgggaa gaaatgatgt tggaaactcc agataaactg gaattcttgt cactgattga
    27661 cattcttgtc gatggaagat atgatcgaac taagagaaat cttatgctcc agtttcgagg
    27721 ttcatctaac caacgaatta tcgatgtgca aaaatcgctc aaaagtgggc aagtagtgat
    27781 ttgggacaag ctcaatgacg gaaaagaaag ctatgaacag gtgaagagag aatgaagaaa
    27841 aaagacttag tagaccaact agtctcagag atcgagacgg ggaaagtcag gacactggga
    27901 atatacggtc atggagcttc aggtaaatca acctttgcac aggaattgta ccaagcttta
    27961 gattctacta cagtaaattt gctagaaaca gatccctata tcacctccgg acgctatttg
    28021 gtagtaccca aggacgcgcc gaatcaaaag gtgacagcca gtctgccagt ggcgcatgaa
    28081 ctggagagtt tgcagagaga tatccttgcc ttgcaggcgg gtatggatgt cttgacaatt
    28141 gaagaacctt ggaaggctag tgaggtcttg tctggagcca aaccaatttt gattgtcgaa
    28201 gggatgtctg ttggttttct acccaaggaa ctctttgaaa aaaccatctg tttctacacg
    28261 gatgaggaga ccgaattaaa gcgacgcctt gctagagata cgactgtgag aaatcgcgat
    28321 gcatccttta tattggctag ccatcagatg agacgggagc agtatctgcg atactataaa
    28381 gaaactgagt ctaaggcgga tatcttagtg gaccaatcag aagataaatt tgatgtcaag
    28441 aggacttaac ttatgtatag aagaaatata gaagaaaatc cctaatttta gaaagaaaaa
    28501 gtaggaaaac agtttcatcc caaaaaaacg aaaaaaccta acaaatccct tgcaatcgca
    28561 ggggatttgt gttattctat tatggtgctg taaattacag ccttagtttt gatgcaagag
    28621 gttgcgacac gctcggttgc attgccacgc aacaccgcgt cggttttctt gtggagctag
    28681 cctattatct taaatagacg aaaaggagaa aaagatggca aacaaaaaaa tccgtatccg
    28741 tttgaaagct tacgaacacc gtacgcttga cacagcggct gcaaaaatcg tagaatcagc
    28801 tactcgtaca ggtgcacaag ttgcgggtcc aatcccactt ccaactgaac gtagcctcta
    28861 cacaatcatt cgtgcgactc acaaatacaa agactctcgc gaacaatttg aaatgcgtac
    28921 acacaaacgt ttgatcgata tcgttaaccc aactcaaaaa acagttgatg ccttgatgaa
    28981 attggatctt ccaagtggtg taaacgtaga aatcaaactt taatctaaaa tataaaagag
    29041 cagaggctgg tgtttcaatc taattgaaca cgggctagac tcggtgtgaa aaagataaac
    29101 ttcctattgt ccgctagaca ctgcgttagt ttcctatttt cactttgagt ttgacgccct
    29161 ttgtatctta gacttgagca taaaaaacgc tcgttaaaaa ctttttgaat aaaaaatata
    29221 gaaaaggaac tattttctca tgacaaaagg aatcttaggg aaaaaagtgg gaatgactca
    29281 aatcttcact gaagctggcg aattgatccc tgtaacagtt attgaagcaa ctccaaacgt
    29341 tgttcttcaa gttaaaactg ttgaaacaga cggatacaac gctatccaag ttggtttcga
    29401 tgacaaacgc gaagtattga gcaacaaacc tgctaaagga catgtagcga aagctaacac
    29461 ggctcctaag cgcttcattc gtgaattcaa aaacgttgaa ggcttggaag ttggtgctga
    29521 aattacagtt gaaacattcg cagctggaga tgttgttgat gtaactggta cttctaaagg
    29581 taaaggtttc caaggtgtta tcaagcgcca cggacaatca cgtggaccga tggctcacgg
    29641 ttctcgttac caccgtcgtc caggttctat gggacctgtt gcacctaacc gcgtattcaa
    29701 aggtaaaaac cttgcaggac gtatgggtgg tgaccgcgta acaattcaaa atcttgaagt
    29761 tgtacaagtt gttccagaaa agaacgttat ccttatcaaa ggtaacgtac caggtgctaa
    29821 gaaatctctt atcactatca aatcagcagt taaagctggt aaataataaa gaaaggggaa
    29881 atcagtcaca atggcaaacg taacattatt tgaccaaact ggtaaagaag ctggccaagt
    29941 tgttcttaac gatgcagtat ttggtatcga accaaatgaa tcagttgtgt ttgatgtaat
    30001 catcagccaa cgcgcaagcc ttcgtcaagg aacacacgct gttaaaaacc gctctgcagt
    30061 atcaggtggt ggacgcaaac cgtggcgtca aaaaggaact ggacgtgctc gtcaaggttc
    30121 tatccgctca ccacaatggc gtggtggtgg tgttgtcttc ggaccaactc cacgttcata
    30181 cggctacaaa cttccacaaa aagttcgtcg cctagctctt aaatcagttt actctgaaaa
    30241 agttgctgaa aacaaattcg tagctgtaga cgctctttca tttacagctc caaaaactgc
    30301 tgaatttgca aaagttcttg cagcattgag catcgattct aaagttcttg ttatccttga
    30361 agaaggaaat gaattcgcag ctctttcagc tcgtaacctt ccaaacgtga aagttgcaac
    30421 tgctacaact gcaagtgttc ttgacatcgc aaatagcgac aaacttcttg tcacacaagc
    30481 agctatctct aaaatcgagg aggttcttgc ataatgaatt tgtatgatgt tatcaaaaaa
    30541 cctgtcatca ctgaaagctc aatggctcaa cttgaagcag ggaaatatgt atttgaagtt
    30601 gacactcgtg cacacaaact tttgatcaag caagctgttg aagctgcttt cgaaggtgtt
    30661 aaagttgcca atgttaacac aatcaacgta aaaccaaaag ctaaacgtgt tggacgttac
    30721 actggtttta ctaacaaaac taaaaaagct atcatcacac ttacagctga ttctaaagca
    30781 atcgagttgt ttgctgctga agctgaataa tctaaggagg aaatatcgtg ggaattcgtg
    30841 tttataaacc aacaacaaac ggtcgccgta atatgacttc tttggatttc gctgaaatca
    30901 caacaagcac tcctgaaaaa tcattgcttg ttgcattgaa gagcaaggct ggtcgtaaca
    30961 acaacggtcg tatcacagtt cgtcaccaag gtggtggaca caaacgtttc taccgtttgg
    31021 ttgacttcaa acgtaataaa gacaacgttg aagcagttgt taaaacaatc gagtacgatc
    31081 caaaccgttc tgcaaacatc gctcttgtac actacactga cggtgtgaaa gcatacatca
    31141 tcgctccaaa aggtcttgaa gtaggtcaac gtatcgtttc aggtccagaa gcagatatca
    31201 aagtcggaaa cgctcttcca cttgctaaca tcccagttgg tactttgatt cacaacatcg
    31261 agttgaaacc aggtcgtggt ggtgaattgg tacgtgctgc tggtgcatct gctcaagtat
    31321 tgggttctga aggtaaatac gttcttgttc gtcttcaatc aggtgaagtt cgtatgattc
    31381 ttggaacttg ccgtgctaca gttggtgttg tcggaaacga acaacatgga cttgtaaacc
    31441 ttggtaaagc aggacgtagc cgttggaaag gtatccgccc aacagttcgt ggttctgtaa
    31501 tgaaccctaa cgatcaccca cacggtggtg gtgaaggtaa agcaccagtt ggtcgtaaag
    31561 caccatctac tccatggggc aaacctgctc ttggtcttaa aactcgtaac aagaaagcga
    31621 aatctgacaa acttatcgtt cgtcgtcgca acgagaaata atattaaact agtcgcttaa
    31681 gcaactagta aatccgccag ctcggtagcg ctccatagga gtgcaagccg ctgtggtaca
    31741 acatttaaag gagaaaatat aaaaatggga cgcagtctta aaaaaggacc tttcgtcgat
    31801 gagcatttga tgaaaaaagt tgaagctcaa gctaacgacg aaaagaaaaa agttattaaa
    31861 acttggtcac gtcgatcaac gatcttccca agtttcattg gttacactat tgcagtttat
    31921 gacggacgta aacacgtacc tgtttacatc caagaagaca tggtaggcca caaacttggt
    31981 gaatttgcac caactcgtac ttacaaaggt cacgctgcag acgacaagaa aacacgtaga
    32041 aaataaggag aacataaatg gcagaaatta cttcagctaa agcaatggct cgtacagtac
    32101 gtgtttcacc tcgtaaatca cgtcttgttc ttgataacat ccgtggtaaa agcgtagccg
    32161 atgcaatcgc aatcttgaca ttcactccaa acaaagctgc tgaaatcatc ttgaaagttt
    32221 tgaactcagc tgtagctaac gctgaaaaca actttggttt ggataaagct aacttggtag
    32281 tatctgaagc attcgcaaac gaaggaccaa ctatgaaacg tttccgtcca cgtgcgaaag
    32341 gttcagcttc accaatcaac aaacgtacag ctcacatcac tgtagctgtt gcagaaaaat
    32401 aaggaggtaa aatcgtgggt caaaaagtac atccaattgg tatgcgtgtc ggcatcatcc
    32461 gtgattggga tgccaaatgg tatgctgaaa aagaatacgc ggattacctt catgaagatc
    32521 ttgcaatccg taaattcgtt caaaaagaac ttgctgacgc agcagtttca actattgaaa
    32581 tcgaacgcgc agtaaacaaa gttaacgttt cacttcacac tgctaaacca ggtatggtta
    32641 tcggtaaagg tggtgctaac gttgatgcac tccgtgcaaa acttaacaaa ttgactggaa
    32701 aacaagtaca catcaacatc atcgaaatca aacaacctga tttggatgct caccttgtag
    32761 gtgaaggaat tgctcgtcaa ttggagcaac gtgttgcttt ccgtcgtgca caaaaacaag
    32821 caatccaacg tgcaatgcgt gctggagcta aaggaatcaa aactcaagta tcaggtcgtt
    32881 tgaacggtgc agatatcgcc cgtgctgaag gatactctga aggaactgtt ccgcttcaca
    32941 cacttcgtgc agatatcgat tacgcttggg aagaagcaga tactacatac ggtaaacttg
    33001 gtgttaaagt atggatctac cgtggtgaag ttcttccagc tcgtaaaaac actaaaggag
    33061 gtaaataacc aatgttagta cctaaacgtg ttaaacaccg tcgtgagttc cgtggaaaaa
    33121 tgcgcggtga agcaaaaggt ggaaaagaag tagcattcgg tgaatacggt cttcaagcta
    33181 caactagcca ctggatcact aaccgccaaa tcgaagctgc tcgtatcgcc atgactcgtt
    33241 acatgaaacg tggtggtaaa gtttggatta aaatcttccc acacaaatca tacactgcta
    33301 aagctatcgg tgtgcgtatg ggatctggta aaggggcacc tgaaggttgg gtagcaccag
    33361 ttaaacgtgg taaagtgatg ttcgaaatcg ctggtgtatc tgaagagatt gcacgtgaag
    33421 cgcttcgact tgctagccac aaattgccag ttaaatgtaa attcgtaaaa cgtgaagcag
    33481 aataaggaga aggcatgaaa cttaatgaag taaaagaatt tgttaaagaa cttcgtggtc
    33541 tttctcaaga agaactcgcg aagcgcgaaa acgaattgaa aaaagaattg tttgaacttc
    33601 gtttccaagc tgctactggt caattggaac aaacagctcg cttgaaagaa gttaaaaaac
    33661 aaatcgctcg tatcaaaaca gttcaatctg aagcgaaata atagactagg gaaggagaaa
    33721 tttcaatgga acgcaataat cgtaaagttc ttgttggacg tgttgtatct gacaaaatgg
    33781 acaagacaat cacagttgta gttgaaacaa aacgtaacca cccagtctat ggtaaacgta
    33841 ttaactactc taaaaaatac aaagctcatg atgaaaacaa tgttgccaaa gaaggcgata
    33901 tcgtacgtat catggaaact cgcccgcttt cagctacaaa acgtttccgt cttgtagaag
    33961 ttgttgaaga agcggtcatc atctaatcaa acctgaaagg agaaaactga aatgattcaa
    34021 acagaaactc gtttgaaagt cgcagacaac agcggtgctc gcgaaatctt gactatcaaa
    34081 gttcttggtg gttcaggacg taaatttgca aacatcggtg atgttatcgt ggcatctgta
    34141 aaacaagcta ctcctggtgg tgcggttaaa aaaggtgacg ttgttaaagc agttatcgtt
    34201 cgtactaaat caggtgctcg tcgtgctgat ggttcataca tcaaatttga cgaaaacgca
    34261 gcagttatca tccgtgaaga caaaactcct cgcggaacac gtatctttgg cccagttgca
    34321 cgtgaattgc gtgaaggtgg cttcatgaag atcgtgtcac ttgctccaga agtactttaa
    34381 tttttaggaa caaactagtc ccctagcttc aagctagggt gcccttatgg gcgtaagaaa
    34441 aatcaaggag aaacctaatg tttgtaaaaa aaggcgacaa agttcgcgta atcgctggta
    34501 aagataaggg aacagaagct gttgtcctta ctgcccttcc aaaagtaaac aaagttatcg
    34561 ttgaaggtgt taacattgtt aagaaacacc aacgtccaac taacgagctt cctcaaggtg
    34621 gtatcatcga gaaagaagca gctatccacg tatcaaacgt tcaagttttg gacaaaaatg
    34681 gtgtagctgg tcgtgttggt tacaaatttg tagacggtaa aaaagttcgc tacaacaaaa
    34741 aatcaggcga agtgcttgat taatcacgaa ggaaaggaga agtataatgg caaatcgttt
    34801 aaaagaaaaa tatcttaatg aagtagttcc tgctttgaca gaacaattca actactcatc
    34861 agtgatggct gtgcctaaag tagataagat tgttttgaac atgggtgttg gtgaagctgt
    34921 atcaaacgct aaaagccttg aaaaagctgc tgaagaattg gcacttatct caggtcaaaa
    34981 accacttatc actaaagcta aaaaatcaat cgccggcttc cgtcttcgtg aaggtgttgc
    35041 gatcggtgca aaagttaccc ttcgtggtga acgtatgtac gaattcttgg ataaattggt
    35101 atcagtttca cttccacgtg tacgtgactt tcacggtgtt ccaacaaaat catttgatgg
    35161 acgcgggaac tacacacttg gtgtgaaaga acaattaatc ttcccagaaa tcaacttcga
    35221 tgacgttgac aaaactcgtg gtcttgacat cgttatcgta acaactgcta acactgacga
    35281 agagtcacgt gcattgctta caggccttgg aatgcctttt gcaaaataat ataggaggta
    35341 aatctaatgg ctaaaaaatc aatggtagct agagaggcta aacgccaaaa aattgttgac
    35401 cgttatgctg aaaaacgtgc tgcattaaag gcggcagggg actacgaagg tttatctaaa
    35461 ttacctcgca acgcctcacc gactcgttta cataatcgtt gtagagttac ggggcgccca
    35521 cattcagttt accgcaaatt tggtctgagt cgtatcgctt ttcgcgaact tgcgcataaa
    35581 ggtcaaattc ctggtgtaac aaaagcatct tggtaattta agatatcaag ggtgtcaaaa
    35641 ctccaagtaa aaataggaaa cttgacgaag aaactaaagt ttctaggaaa gtttatcttt
    35701 ttcacacaga gtttagcccg ggttcaattg ggcttgccaa tttgaacccg agctacagct
    35761 ttggcaaaaa agaccaattt gctttggagc attgcttctg cattaaattg tctatttttg
    35821 ctcgtgctgt tacgctcttt gtatcatgta ttaactagca agtgcaactt gcaaactact
    35881 agtaagagga gaaaaacaaa atggttatga ctgacccaat cgcagacttc ctaactcgta
    35941 ttcgtaatgc taaccaagct aaacacgaag tacttgaagt acctgcatca aacatcaaaa
    36001 aagggattgc tgaaatcctt aaacgcgaag gttttgtaaa aaacgttgaa atcatcgaag
    36061 atgacaaaca aggcgtcatc cgtgtatttc ttaaatacgg accaaatggt gaaaaagtta
    36121 tcactaactt gaaacgtgtt tctaaaccag gacttcgtgt ctacaaaaaa cgtgaagacc
    36181 ttccaaaagt tcttaacgga cttggaattg ccatcctttc aacttctgaa ggtttgctta
    36241 ctgataaaga agcacgccaa aagaatgttg gtggtgaggt tatcgcttac gtttggtaaa
    36301 atcaagatac aaagctcgta aagaacaaag caaaattagg aagttggaga agtttgttta
    36361 caaacaagcc aacttatcta ttttgcacag ttcttagaac gtgttcagtt cagctcttga
    36421 actaaataag tatctgaacc ccgtgaaaac tggccgttct ggcctgacaa tttaacagga
    36481 gaaaataaac atgtcacgta ttggtaataa agttatcgtg ttgcctgctg gtgttgaact
    36541 cgctaacaat gacaacgttg taactgtaaa aggacctaaa ggagaactta ctcgtgagtt
    36601 ctcaaaagat attgaaatcc gtgtggaagg tactgaaata actcttcacc gtccaaacga
    36661 ttcaaaagaa atgaaaacta tccacggaac tactcgtgcc cttttgaaca acatggttgt
    36721 tggtgtatca gaaggattca agaaagaact tgaaatgcgt ggggttggtt accgtgcaca
    36781 gcttcaagga tctaaacttg ttttggctgt tggtaaatct catccagacg aagttgaagc
    36841 tccagaagga attacttttg aacttccaaa cccaacaaca atcgttgtta gcggaatttc
    36901 aaaagaagta gttggtcaaa cagctgctta cgtacgtagc cttcgttcac cagaaccata
    36961 taaaggtaaa ggtatccgtt acgttggtga attcgttcgc cgtaaagaag gtaaaacagg
    37021 taaataatgt tgagtggttg atcatcaacc accaacctat tttccaactt tgtgcatagc
    37081 acacgattta aaactaaaga ggtgaaaact gtgatttcaa aaccagataa aaacaaactc
    37141 cgccaaaaac gccaccgtcg cgttcgcgga aaactctctg gaactgctga tcgcccacgt
    37201 ttgaacgtat tccgttctaa tacaggcatc tacgctcaag tgattgatga cgtagcgggt
    37261 gtaacgctcg caagtgcttc aactcttgat aaagaagttt caaaaggaac taaaactgaa
    37321 caagccgttg ctgtcggtaa actcgttgca gaacgtgcaa acgctaaagg tatttcagaa
    37381 gtggtgttcg accgcggtgg atatctatat cacggacgtg tgaaagcttt ggctgatgca
    37441 gctcgtgaaa acggattgaa attctaatag gaggacacta gaaaatggca tttaaagaca
    37501 atgcagttga attagaagaa cgcgtagttg ctgtcaaccg tgttacaaaa gttgttaaag
    37561 gtggacgtcg tcttcgtttc gcagctcttg ttgttgttgg tgaccacaat ggtcgcgtag
    37621 gatttggtac tggtaaagct caagaagttc cagaagcaat ccgtaaagca gtagatgatg
    37681 ctaagaaaaa cttgatcgaa gttcctatgg ttggaacaac aatcccacac gaagttcttt
    37741 cagaattcgg tggagctaaa gtattgttga aacctgctgt agaaggttct ggagttgccg
    37801 ctggtggtgc agttcgtgcc gttgtggaat tggcaggtgt ggcagatatt acatctaaat
    37861 cacttggttc taacactcca atcaacattg ttcgtgcaac tgttgaaggt ttgaaacaat
    37921 tgaaacgcgc tgaagaaatt gctgcccttc gtggtatttc agtttctgat ttggcataag
    37981 aaaggggata aaatggctca aattaaaatt actttgacta agtctccaat cggacgcatt
    38041 ccatcacaac gtaaaactgt tgtagcactt ggacttggca aattgaacag ctctgttatt
    38101 aaagaagata acgctgctat ccgtggtatg attacagcag tatctcactt agtaacagtt
    38161 gaagaagtaa actaatgaag ttttagggga tgtgcactgt accatcccct aaaactagat
    38221 atagtcatct atgatgacat cgtataggcg agttgatggg ggagacaacc ttttctccct
    38281 tatcggcgct agcattttac aaaagaggag aaaataaaaa tgaaacttca tgaattgaaa
    38341 cctgcagaag gttctcgtaa agtacgtaac cgcgttggtc gtggtacttc atcaggtaac
    38401 ggtaaaacat ctggtcgtgg tcaaaaaggt caaaaagctc gtagcggtgg cggagttcgc
    38461 cttggttttg aaggtggaca aactccattg ttccgtcgtc ttccaaaacg tggattcact
    38521 aacatcaacg ctaaagaata cgcaattgtg aaccttgacc aattgaacgt ctttgaagat
    38581 ggtgctgaag taactccagt tgttcttatc gaagcaggaa ttgttaaagc tgaaaagtca
    38641 ggtattaaaa ttcttggtaa cggtgagttg actaagaaat tgactgtgaa agcagctaaa
    38701 ttctctaaat cagctgaaga agctatcact gctaaaggtg gttcagtaga agtcatctaa
    38761 gagaggtgac ctatgttttt taaattatta agagaagctc ttaaagtcaa gcaggttcga
    38821 tcaaaaattt tatttacaat ttttatcgtt ttggtctttc gtatcggaac tagcattaca
    38881 gttcctggtg tgaatgccaa tagcttgaat gctttaagtg gattatcctt cttaaacatg
    38941 ttgagcttgg tgtcggggaa tgccctaaaa aacttttcga tttttgccct aggagttagt
    39001 ccctatatca ccgcttctat tgttgtccaa ctcttgcaaa tggatatttt acccaagttt
    39061 gtagagtggg gtaaacaagg ggaagtaggt cgaagaaaat tgaatcaagc tactcgttat
    39121 attgctctag ttctcgcttt tgtgcaatct atcgggatta cagctggttt taataccttg
    39181 gctggagctc aattgattaa aactgcttta actccacaag tttttctgac gattggtatc
    39241 atcttaacag ctggtagtat gattgtcact tggttgggtg agcaaattac agataaggga
    39301 tacggaaacg gtgtttccat gattatcttt gccgggattg tttcctcaat tccagagatg
    39361 attcagggca tctatgtgga ctactttgtg aacgtcccaa gtagccgtat cacttcatct
    39421 atcattttcg taatcatttt gattattact gtattgttga ttatttactt tacaacttat
    39481 gttcaacaag cagaatacaa aattccaatc caatatacta aggttgcaca aggtgctcca
    39541 tctagctctt accttccgtt aaaggtaaac cctgctggag ttatccctgt tatctttgcc
    39601 agttcgatta ctgcagcgcc tgcggctatt cttcagtttt tgagtgccac aggtcatgat
    39661 tgggcttggg taagggtagc acaagagatg ttggcaacta cttctccaac tggtattgcc
    39721 atgtatgctt tgttgattat tctctttaca ttcttctata cgtttgtaca gattaatcct
    39781 gaaaaagcag cagagagcct acaaaagagt ggtgcctata tccatggagt tcgtcctggt
    39841 aaaggtacag aagaatatat gtctaaactt cttcgtcgtc ttgcaactgt tggttccctc
    39901 ttccttggtg tgatttccat tttaccgatt gcagctaaag atgtatttgg tctttctgat
    39961 gttgttgcct ttggtggaac aagtctcttg atcattatct ctacaggtat cgaaggaatc
    40021 aagcaattgg aaggttacct attgaaacgt aagtatgttg gtttcatgga cagaacagaa
    40081 taaaagtatt tactgaatca gtaaatactg agggagtgga ggtttaaact ctgacatttg
    40141 taagagttgg atctcccctc ttctattttg tttttaaata ggggtgaaaa gactttttgc
    40201 ttctatttaa aaataaaata aggagatcaa atcatgaatc ttttgattat gggcttacct
    40261 ggtgcaggta agggaactca agcagcaaaa atcgtagaac aattccatgt tgcacatatc
    40321 tcaacaggtg atatgttccg cgctgcaatg gcaaatcaaa ctgaaatggg tgttcttgct
    40381 aagtcatata ttgacaaggg tgaattggtt cctgacgaag ttacaaatgg aatcgtaaaa
    40441 gaacgccttt cacaagatga tattaaagaa acaggattct tattggatgg ttacccacgt
    40501 acaattgaac aagctcatgc cttggacaaa acattggctg aacttggcat tgaactagaa
    40561 ggtattatca atattgaagt gaaccctgac agcctcttgg aacgtttgag tggccgtatc
    40621 atccaccgcg taactggaga aactttccac aaggtcttta acccaccagt tgactataaa
    40681 gaagaagatt actaccaacg tgaagatgat aagcctgaga cagtaaaacg tcgtttggat
    40741 gttaatattg ctcaaggaga accaatcatt gctcactacc gtgccaaagg tttggttcat
    40801 gacatcgaag gtaatcaaga tatcaatgat gtcttctcag atattgaaaa agtattgaca
    40861 aatttgaaat aaagcgtttt tcgcacttgc aaaaatccgc tacaaatgtt atactgagat
    40921 agtctgactt ataattgttg tctctgtgtc tagaggcatc gaatcgaaat ttatggaggt
    40981 gcttttgcgt ggcaaaagac gatgtgattg aagttgaagg caaagtagtt gatacaatgc
    41041 cgaatgcaat gtttacggtt gaacttgaaa atggacatca gattttagca acagtttctg
    41101 gtaaaattcg taaaaactat attcgtattt tagcgggaga tcgtgttact gtcgaaatga
    41161 gtccatatga cttgacacgt ggacgtatca cttaccgctt taaataatcg aaaaacttgg
    41221 agggataaga aatgaaagta agaccatcgg tcaaaccaat ttgcgaatac tgtaaagtta
    41281 ttcgtcgtaa tggtcgtgtt atggtaattt gcccagcaaa tccaaaacac aaacaacgtc
    41341 aaggataaga tagaaaggag aaaacatggc tcgtattgct ggagttgata ttccaaatga
    41401 caaacgcgta gtaatctcat tgacttatgt ttatggtatc ggacttgcaa catctaagaa
    41461 aattttggct gctgctggaa tctcagaaga tgttcgtgta cgtgatctta catcagatca
    41521 agaagatgct atccgtcgtg aagtggatgc aatcaaagtt gaaggtgacc ttcgtcgtga
    41581 agtaaacttg aacatcaaac gtttgatgga aatcggttca taccgtggta tccgtcaccg
    41641 tcgtggactt cctgtccgtg gacaaaacac taaaaacaac gcccgcactc gtaaaggtaa
    41701 agctgttgcg attgctggta agaaaaaata atataggagg taaaagtctt ggctaaacca
    41761 acacgtaaac gtcgtgtgaa aaagaatatc gaatctggta ttgctcatat tcacgctaca
    41821 tttaataaca ctattgttat gattactgat gtgcatggta atgcaattgc ttggtcatca
    41881 gctggtgctc ttggtttcaa aggttctcgt aaatctacac cattcgctgc tcaaatggct
    41941 tctgaagctg ctgctaaatc tgcacaagaa cacggtctta aatcagttga agttactgta
    42001 aaaggtccag gttctggtcg tgagtcagct attcgtgcgc ttgctgccgc tggtcttgaa
    42061 gtaacagcaa ttcgtgatgt gactccagtg ccacacaatg gtgctcgtcc tccaaaacgt
    42121 cgccgtgtat aatcatcgca ttacactgct tttcgtttaa gagggagtaa ctaaatgatc
    42181 gagtttgaaa aaccaaatat aacaaaaatt gatgaaaata aagattatgg caagtttgta
    42241 atcgaaccac ttgaacgtgg ctacggtaca actcttggta actctcttcg tcgtgtactt
    42301 ctagcttctc taccaggagc agctgtgaca tctatcaaca ttgatggtgt gttacatgag
    42361 tttgacacag ttccaggtgt tcgtgaagac gttatgcaaa tcattctgaa cattaaagga
    42421 attgcagtga aatcgtacgt tgaagacgaa aaaatcatcg aactggatgt tgaaggtcct
    42481 gctgaagtaa cagctggtga cattttgaca gatagcgata ttgaaattgt aaatccagat
    42541 cattatctct ttacaattgg tgaaggttct tctctaaaag cgactatgac tgttaacagt
    42601 ggtcgtggat atgtacctgc tgatgaaaat aaaaaggata atgcaccagt tggaacactt
    42661 gctgtagatt ctatttatac accagttaca aaagtcaact atcaagtgga acctgctcgt
    42721 gtaggtagca atgatggttt cgacaaatta acccttgaaa tcttgacaaa tggaacaatt
    42781 attccagaag atgctttagg gctttcagca cgtattttga cagaacatct tgatttgttt
    42841 acaaatctta ctgagattgc taagtcaact gaagtgatga aagaagctga tactgaatct
    42901 gacgaccgta ttttagatcg tacgattgag gaactggact tgtctgtgcg ttcatacaac
    42961 tgtttaaaac gtgccggtat caatactgtg catgatttga cagaaaaatc tgaagcagag
    43021 atgatgaaag tacgaaatct tggacgcaag agtttggaag aagtgaaact caaactcatt
    43081 gatttgggtc ttggattaaa agataaataa aggaggaata catggcttac cgtaaactag
    43141 gacgcactag ctcacaacgt aaagcaatgc ttcgcgattt gacaactgac cttttgatca
    43201 acgaatcaat cgtgacaact gaagctcgtg ctaaagaaat ccgtaaaact gttgaaaaaa
    43261 tgattactct aggtaaacgt ggtgatttgc atgcacgtcg tcaagcagct gctttcgtac
    43321 gtaatgaaat cgcatctgaa aactatgatg aagcaactga taagtacact tctactacag
    43381 cacttcaaaa attgttctca gaaatcgcac ctcgttatgc tgaacgtaac ggtggataca
    43441 ctcgtatcct taaaactgaa ccacgtcgtg gtgatgcagc gccaatggcg atcatcgaat
    43501 tagtataaaa tcatcaattt tgttgagtgt tatgatgatg gagtcttgtg ctcttagtct
    43561 agctctggtc taccgctagg atttcggtcc tagcgggaac actcatcata agttgggata
    43621 gtagacgctt gtttacgaaa ttgttttttt cttaagaaca acttcgtaag caggcgtttt
    43681 tgagtatttt cgttagaatt atgctatact atttgaaaag aatcctgttt aatgttaagg
    43741 tttcttattt taagaagaat tggagtttac ttatgaaagc gattataact gttgttggta
    43801 aagataaatc tggaattgtt gcaggtgttt ctggtaaaat tgcagaatta ggattgaata
    43861 ttgacgatat ctctcaaact gtattggatg aatattttac gatgatggct gttgtatcta
    43921 gtgatgaaaa gcaagatttt acctatcttc gtaatgaatt tgaagctttt gggcaaactt
    43981 tgaatgtaaa aatcaatatt cagagtgcag cgattttcga agctatgtat aatatctagg
    44041 aggtcatcat ggatattaga caagttactg aaaccatcgc catgattgag gagcaaaact
    44101 tcgatattag aaccattacc atggggattt ctcttttgga ctgtatcgat ccaaatatca
    44161 atcgtgctgc ggagaaaatc tatcaaaaaa ttacgacaaa ggcggctaat ttagtagctg
    44221 ttggtgatga aattgcggct gagttgggaa ttcctatcgt taataagcgt gtatcggtga
    44281 cacctatttc tctgattggg gcagcgacag atgcgacgga ctacgtggtt ctggcaaaag
    44341 cgcttgataa ggctgcgaaa gagattggtg tggactttat tggtggtttt tctgccttag
    44401 tacaaaaagg ttatcaaaag ggagatgaga ttctcatcaa ttccattcct cgcgctttgg
    44461 ctgagacgga taaggtctgc tcgtcagtca atatcggctc aaccaagtct ggtattaata
    44521 tgacggctgt ggcagatatg ggacgaatta tcaaggaaac agcaaatctt tcagatatgg
    44581 gagtggccaa gttggttgta ttcgctaatg ctgttgagga caatccattt atggcgggtg
    44641 cctttcatgg tgttggggaa gcagatgtta tcatcaatgt cggagtttct ggtcctggtg
    44701 ttgtgaaacg tgctttggaa aaagttcgtg gacagagctt tgatgtagta gccgaaacag
    44761 ttaagaaaac tgcctttaaa atcactcgta tcggtcaatt ggttggtcaa atggccagtg
    44821 agagactggg tgtggagttt ggtattgtgg acttgagttt ggcaccaacc cctgcggttg
    44881 gagactctgt ggcacgtgtc cttgaggaaa tggggctaga aacagttggc acgcatggaa
    44941 cgacagctgc cttggccctc ttgaacgacc aagttaaaaa gggtggagtg atggcctgta
    45001 accaggtcgg tggtctatct ggtgccttta tccctgtttc tgaggatgaa ggaatgattg
    45061 ctgcagtgca aaatggctct cttaatttag aaaaactaga agctatgacg gctatctgtt
    45121 ctgttggatt ggatatgatt gccatcccag aagatacgcc tgctgaaact attgcggcta
    45181 tgattgcgga tgaagcagca atcggtgtta tcaacatgaa aacaacagct gttcgtatca
    45241 ttcccaaagg aagagaaggc gatatgattg agtttggtgg tctattagga actgcacccg
    45301 ttatgaaggt taatggggct tcgtctgtcg acttcatctc tcgcggtgga caaatcccag
    45361 caccaattca tagttttaaa aattaagaaa ataggagaaa ttttaagttc tatttaagat
    45421 tagatgtg
//
LOCUS       DAVLSL010000002        40283 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLSL010000002 DAVLSL010000000
VERSION     DAVLSL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.002"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            3..75
                     /locus_tag="VXN30_000061"
     tRNA            3..75
                     /locus_tag="VXN30_000061"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Val,seq:tac)
     gene            81..151
                     /locus_tag="VXN30_000062"
     tRNA            81..151
                     /locus_tag="VXN30_000062"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:113..115,aa:Gly,seq:tcc)
     gene            186..259
                     /locus_tag="VXN30_000063"
     tRNA            186..259
                     /locus_tag="VXN30_000063"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:Ile,seq:gat)
     gene            342..431
                     /locus_tag="VXN30_000064"
     tRNA            342..431
                     /locus_tag="VXN30_000064"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:378..380,aa:Ser,seq:tga)
     gene            442..515
                     /locus_tag="VXN30_000065"
     tRNA            442..515
                     /locus_tag="VXN30_000065"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Met,seq:cat)
     gene            519..591
                     /locus_tag="VXN30_000066"
     tRNA            519..591
                     /locus_tag="VXN30_000066"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:552..554,aa:Phe,seq:gaa)
     gene            604..684
                     /locus_tag="VXN30_000067"
     tRNA            604..684
                     /locus_tag="VXN30_000067"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:638..640,aa:Tyr,seq:gta)
     gene            689..759
                     /locus_tag="VXN30_000068"
     tRNA            689..759
                     /locus_tag="VXN30_000068"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:721..723,aa:Trp,seq:cca)
     gene            774..846
                     /locus_tag="VXN30_000069"
     tRNA            774..846
                     /locus_tag="VXN30_000069"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:807..809,aa:His,seq:gtg)
     gene            860..931
                     /locus_tag="VXN30_000070"
     tRNA            860..931
                     /locus_tag="VXN30_000070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:892..894,aa:Gln,seq:ttg)
     gene            941..1024
                     /locus_tag="VXN30_000071"
     tRNA            941..1024
                     /locus_tag="VXN30_000071"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:975..977,aa:Leu,seq:caa)
     gene            1131..2408
                     /locus_tag="VXN30_000072"
     CDS             1131..2408
                     /locus_tag="VXN30_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1356755.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2484..3968
                     /locus_tag="VXN30_000073"
     CDS             2484..3968
                     /locus_tag="VXN30_000073"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1356756.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            4029..5309
                     /locus_tag="VXN30_000074"
     CDS             4029..5309
                     /locus_tag="VXN30_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1356757.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVLIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5313..5933
                     /locus_tag="VXN30_000075"
     CDS             5313..5933
                     /locus_tag="VXN30_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1356758.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6099..7211
                     /locus_tag="VXN30_000076"
     CDS             6099..7211
                     /locus_tag="VXN30_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1356759.1"
                     /translation="MKKRMLLASTVTLSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRSY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7534..7959
                     /locus_tag="VXN30_000077"
     CDS             7534..7959
                     /locus_tag="VXN30_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1356760.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7970..8329
                     /locus_tag="VXN30_000078"
     CDS             7970..8329
                     /locus_tag="VXN30_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1356761.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8436..9080
                     /gene="pcp"
                     /locus_tag="VXN30_000079"
     CDS             8436..9080
                     /gene="pcp"
                     /locus_tag="VXN30_000079"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1356762.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9108..9965)
                     /locus_tag="VXN30_000080"
     CDS             complement(9108..9965)
                     /locus_tag="VXN30_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1356763.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10095..11237
                     /gene="tgt"
                     /locus_tag="VXN30_000081"
     CDS             10095..11237
                     /gene="tgt"
                     /locus_tag="VXN30_000081"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1356764.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11335..13152
                     /locus_tag="VXN30_000082"
     CDS             11335..13152
                     /locus_tag="VXN30_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1356765.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13305..14456
                     /gene="nagA"
                     /locus_tag="VXN30_000083"
     CDS             13305..14456
                     /gene="nagA"
                     /locus_tag="VXN30_000083"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1356766.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14619..15677
                     /locus_tag="VXN30_000084"
     CDS             14619..15677
                     /locus_tag="VXN30_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1356767.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15828..16193
                     /locus_tag="VXN30_000085"
     CDS             15828..16193
                     /locus_tag="VXN30_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1356768.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16268..17209
                     /locus_tag="VXN30_000086"
     CDS             16268..17209
                     /locus_tag="VXN30_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1356769.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17157..18173
                     /locus_tag="VXN30_000087"
     CDS             17157..18173
                     /locus_tag="VXN30_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1356770.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18175..18501
                     /locus_tag="VXN30_000088"
     CDS             18175..18501
                     /locus_tag="VXN30_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1356771.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18494..18898
                     /locus_tag="VXN30_000089"
     CDS             18494..18898
                     /locus_tag="VXN30_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1356772.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18903..19163
                     /locus_tag="VXN30_000090"
     CDS             18903..19163
                     /locus_tag="VXN30_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356773.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19126..19587
                     /locus_tag="VXN30_000091"
     CDS             19126..19587
                     /locus_tag="VXN30_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1356774.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19565..20011
                     /locus_tag="VXN30_000092"
     CDS             19565..20011
                     /locus_tag="VXN30_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1356775.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNTLNPE"
     gene            20114..20701
                     /locus_tag="VXN30_000093"
     CDS             20114..20701
                     /locus_tag="VXN30_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1356776.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            20762..21715
                     /locus_tag="VXN30_000094"
     CDS             20762..21715
                     /locus_tag="VXN30_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1356777.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21766..22956
                     /locus_tag="VXN30_000095"
     CDS             21766..22956
                     /locus_tag="VXN30_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1356778.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23105..23476
                     /gene="rnpA"
                     /locus_tag="VXN30_000096"
     CDS             23105..23476
                     /gene="rnpA"
                     /locus_tag="VXN30_000096"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1356779.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVN"
     gene            23451..24275
                     /locus_tag="VXN30_000097"
     CDS             23451..24275
                     /locus_tag="VXN30_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1356780.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24294..25280
                     /locus_tag="VXN30_000098"
     CDS             24294..25280
                     /locus_tag="VXN30_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1356781.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25331..25954
                     /locus_tag="VXN30_000099"
     CDS             25331..25954
                     /locus_tag="VXN30_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1356782.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26085..27542
                     /locus_tag="VXN30_000100"
     CDS             26085..27542
                     /locus_tag="VXN30_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1356783.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLAAYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27565..27846
                     /locus_tag="VXN30_000101"
     CDS             27565..27846
                     /locus_tag="VXN30_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1356784.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQRDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27922..28407
                     /locus_tag="VXN30_000102"
     CDS             27922..28407
                     /locus_tag="VXN30_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1356785.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28424..29089
                     /locus_tag="VXN30_000103"
     CDS             28424..29089
                     /locus_tag="VXN30_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1356786.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29093..29956
                     /locus_tag="VXN30_000104"
     CDS             29093..29956
                     /locus_tag="VXN30_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1356787.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29958..30662
                     /locus_tag="VXN30_000105"
     CDS             29958..30662
                     /locus_tag="VXN30_000105"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1356788.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYRAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30841..32514
                     /locus_tag="VXN30_000106"
     CDS             30841..32514
                     /locus_tag="VXN30_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1356789.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEQEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32626..33717
                     /gene="ulaG"
                     /locus_tag="VXN30_000107"
     CDS             32626..33717
                     /gene="ulaG"
                     /locus_tag="VXN30_000107"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1356790.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33831..35807
                     /gene="tkt"
                     /locus_tag="VXN30_000108"
     CDS             33831..35807
                     /gene="tkt"
                     /locus_tag="VXN30_000108"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1356791.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35924..36223
                     /gene="yajC"
                     /locus_tag="VXN30_000109"
     CDS             35924..36223
                     /gene="yajC"
                     /locus_tag="VXN30_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1356792.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36269..36697
                     /locus_tag="VXN30_000110"
     CDS             36269..36697
                     /locus_tag="VXN30_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1356793.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNVSDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36699..37109
                     /locus_tag="VXN30_000111"
     CDS             36699..37109
                     /locus_tag="VXN30_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356794.1"
                     /translation="MENLTNFYEKYRVYLTRPRLEILTVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37409..40060
                     /gene="adhE"
                     /locus_tag="VXN30_000112"
                     /gene_synonym="adhC"
     CDS             37409..40060
                     /gene="adhE"
                     /locus_tag="VXN30_000112"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1356795.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12218 a         7232 c         9090 g        11743 t
ORIGIN      
        1 tagggagttt agctcagctg ggagagcatc tgccttacaa gcagagggtc agcggttcga
       61 tcccgttaac tcccatttta gcgggtgtag tttagtggta aaactacagc cttccaagct
      121 gttgtcgcga gttcgattct cgtcacccgc tttgaacttt gttctttgta ccaagttttt
      181 gacttgggcg cgtagctcag gtggttagag cgcacgcctg ataagcgtga ggtcggtggt
      241 tcgagtccac tcgtgcccat agtgtttagt ccattactag gggattggaa tattatctgt
      301 tcactaagag gacacgggct tgttcccgta taaactattt tggaggatta cccaagtccg
      361 gctgaaggga acggtcttga aaaccgtcag gcgtgtaaaa gcgtgcgtgg gttcgaatcc
      421 cacatcctcc ttttatatta acgcgggatg gagcagctcg gtagctcgtc gggctcataa
      481 cccgaaggtc gtaggttcaa atcctgctcc cgcaataagg ctcggtagct cagttggtag
      541 agcaatggat tgaagctcca tgtgtcggcg gttcgattcc gtctcgcgcc atttatatat
      601 tttggaaggg tagcgaagag gctaaacgcg gcggactgta aatccgctcc ttcgggttcg
      661 ggggttcgaa tccctcccct tccattttac gggcatagtt taaaggtaga actaaggtct
      721 ccaaaacctt cagtgtgggt tcaattccta ctgcccgtgt taatagaatt atggcgggtg
      781 tggtgaagtg gttaacacac cagattgtgg ctctggcatg cgtgggttcg atccccatca
      841 ctcgcctatt ttatattatt ggggtatagc caagcggtaa ggcaagggac tttgactccc
      901 tcatgcgttg gttcgaatcc agctacccca gttactattt gccggcgtgg cggaattggc
      961 agacgcgctg gactcaaaat ccagtgtccg caaggacgtg ccggttcgac cccggccgcc
     1021 ggtatagtat agtgttagga acgttgttat tcttcgttcc ttttttatat tatttttggt
     1081 ataattatag ttattcaaat tttatttaga ttaagaaagt gtaggggagt atgtcttgtt
     1141 ctatcgattt attaaaacat cggtatttga aaaatattaa agaaaatcct gaattgtttg
     1201 tcggaattga gttggagtat cctgttgcaa gtttagaagg ggatgctaca gatgttgaag
     1261 ttatgaagga tctatttcat tatttagttt ctactttgga tctcaccgta gcaaaggtag
     1321 atgattttgg caatctgatc cagttagtag atccgataag tcaggatgct attttatttg
     1381 aagtttccta tacaacgatt gagtttgcat ttggtaaggc tgaaacgatt caagaggtcg
     1441 aaaatcgttt caataattat atgaatgtaa ttcagagaaa gttagctgaa tcaaatcatg
     1501 ctattgttgg ctgtggtatc catcccaact gggataaaaa tgagaattgt ccagtggctt
     1561 atccacgcta tcagatgttg atggattatt tgaatttgag tagaaatatt attaaatcag
     1621 atttacatca tttccctgaa tatggtactt ttatctgtgg gagccaggtt cagctggata
     1681 tttcaaaaac caactactta cgggtgatta atgcttttac tcaaattgaa gcggctaagg
     1741 cttatttatt tgcaaactct gaattttcgg gtgcggattg ggatacgaaa atttcaaggg
     1801 atattttctg ggaagaatct atgcatggta tctatccaga gaatgttggg gtcaatgcta
     1861 gactccttaa tgatgaagct gatttttttg actatctaaa tcattctgcg atttttactg
     1921 cggaacgtga tgggcagacc tattattttt atcctattca ggctggggac tatttggcta
     1981 cgtccgaaat ccaagcattt gctctgaatg gggatgaggt tattatttac ccccaagaga
     2041 aggattttga aactcatcgt agttaccagt accaagattt aacgactcga ggaacagttg
     2101 agtttcgtag tgtgtgtaca cagccacttg ataggacttt tgcttctgca gcttttcact
     2161 tgggattatt ggttaattta gacaagttag aagcttactt agaaacagca cctttcttta
     2221 aagtatttgg ttatgattac aagtctttaa ggagacaatt ttctaagaaa aatcttacag
     2281 atgaggaaga aactacgatt attgaatttt ccaaagactt actcctacta gctgaggagg
     2341 gactagtggt gagaaataag gaagaaatga cctatttaca gcctttgaga gaagaattga
     2401 gcctataatt tctcttataa agggagaatt ttctgaaaaa tcatgatata atggacgaga
     2461 ctatagataa aggatagaga gtaatgacat tagtttatca atcaacgcgt gatgccaaca
     2521 atacagtaac tgccagccaa gcaattttgc aaggtttggc gacggacggc ggtttgttta
     2581 caccggatac ttatccaaag gtagatttga actttgacaa attgaaagat gcttcttacc
     2641 aggaagttgc taagctagtt ttgtcagcat ttttagatga ctttacagtt gaggagttgg
     2701 actactgtat caacaatgcc tacgatagca aatttgatac tccagctatt gcaccattag
     2761 tgaaattaga tgggcaatac aatttggaac ttttccatgg ttcaacgatt gcctttaagg
     2821 atatggcctt gtctattttg ccatacttta tgacgactgc tgctaagaaa catggtttgg
     2881 agaacaagat tgttatcttg acagcgacat ctggtgacac ggggaaagct gctatggcgg
     2941 ggtttgcgaa tgtgcctggt actgagatta tcgtctttta tccaaaggat ggtgtcagca
     3001 agattcaaga gttacaaatg accactcaga ctggcgacaa tactcatgtt attgctattg
     3061 atggtaactt tgacgatgcg caaacaaatg tgaagcacat gtttaacgac gtggctcttc
     3121 gtgaaaaatt gactaccaac aagttgcaat tttcatcagc taactctatg aacattggtc
     3181 gtctggtgcc acaaattgtt tattatgttt atgcttacgc tcaattggtt aagactggtg
     3241 aaattgtagc tggtgaaaag gttaacttca cagtaccaac aggaaacttt ggaaatatct
     3301 tggctgcctt ttatgccaaa caaattggtt tgccagttgg taaattaatc tgtgcttcaa
     3361 atgacaacaa tgttttgaca gacttcttta aaacacgtgt ctatgacaaa aaacgtgagt
     3421 ttaaggtaac aaccagccca tctatggata tcttggtatc ttcaaacttg gagcgcttga
     3481 ttttccatct tttgggaaat aatgctgaaa agacaactga acttatgaat gccttgaaca
     3541 cgcaaggaca atataagttg acagactttg atgcagagat tttggacctc tttgcagctg
     3601 aatatgcgac tgaggaagaa acggcagcag agatcaagcg tgtttgtgag ttagattctt
     3661 atatcgagga ccctcataca gctgttgctt cagcagttta taaaaaatac caatcggcca
     3721 ctggagatgt aactaagaca gtgattgctt caacagctag tccatacaag ttcccagtag
     3781 ttgcagtaga agctgtaact ggaaaagcag gtttaacaga ctttgaagcc ttggctcaat
     3841 tacatgaaat ctcaggcgtt gcagtgccac cagcagttga tgggcttgaa atagctccaa
     3901 ttcgtcacaa gacaacagtg gcagctgctg acatgcaagc agcggttgag gcttatttag
     3961 gactttaaga cagagggagc aaactcggtt gggaaaccaa ctgagtttct tttcatcagg
     4021 aggagagatt gtttaagaaa aataaagaca ttcttaatat tgcattgcca gctatgggtg
     4081 aaaacttttt gcagatgcta atgggaatgg tggacagtta tttggttgct catttaggat
     4141 tgatagctat ttcaggggtt tcagtagctg gtaatattat caccatttat caggcgattt
     4201 tcatcgctct gggagctgct atttccagtg ttatttcaaa aagcataggg cagaaagacc
     4261 agtcgaagtt ggcctatcat gtgactgagg cgttgaagat taccttacta ttaagtttcc
     4321 ttttaggatt tttgtccatc ttcgctggga aagagatgat aggacttttg gggacggaga
     4381 gggatgtagc tgagagtggt ggactgtatc tatctttggt aggcggatcg attgttctct
     4441 taggtttaat gactagtcta ggagccttga ttcgtgcaac gcataatcca cgtctgcctc
     4501 tctatgttag ttttttatcc aatgccttga atattctttt ttcaagtcta gctatttttg
     4561 ttctggatat ggggatagct ggtgttgctt gggggacaat tgtgtctcgt ttggttggtc
     4621 ttgtgatttt gtggtcacaa ttaaaactgc cttatgggaa gccaactttt ggtttagata
     4681 aggaactgtt gaccttggct ttaccagcag ctggagagcg acttatgatg agggctggag
     4741 atgtagtgct cattgccttg gtcgtttctt ttgggacgga ggcagttgct gggaatgcaa
     4801 tcggagaagt cttgacccag tttaactata tgcctgcctt tggcgtcgct acggcaacgg
     4861 tcatgctgtt ggcccgagca gttggagagg atgattggaa aagagttgct agtttgagta
     4921 aacaaacctt ttggctttct ctgttcctca tgttgcccct gtcctttagt atatatgtct
     4981 tgggtgtacc attaactcat ctctatacga ctgattctct agcggtggag gctagtgttc
     5041 tagtgacact gttttcacta cttgggaccc ctatgacgac aggaacagtc atctatacgg
     5101 cagtctggca gggattagga aatgcacgcc tcccttttta tgcgacaagt ataggaatgt
     5161 ggtgtatccg cattgggaca ggatatctga tggggattgt gcttggttgg ggcttgcctg
     5221 gtatttgggc agggtctctc ttggataatg gttttcgctg gttatttcta cgctatcgtt
     5281 accagcgcta tatgagcttg aaaggatagg aaatgcaaaa aacagctttt atttgggatt
     5341 tagacgggac tttattggac tcttacgaag cgattttatc agggattgag gagacttttg
     5401 ctcagttttc tattccttat gataaggaga aggtgagaga gtttatcttc aagtattcgg
     5461 tgcaagattt gcttgtgcgg gtggcagaag atagaaatct ggatgttgag gtgctaaatc
     5521 aggtgcgtgc ccagagtctg gctgagaaga atgctcaggt agttttgatg ccaggtgcgc
     5581 gtgaggtgct agcttgggca gacgaatcag gaattcagca gtttatatat actcataagg
     5641 ggaacaacgc ttttaccatt ctcaaggact tgggggtgga atcctatttt acagagattt
     5701 taaccagtca gagtggcttt gtgcggaagc caagtccaga agcggctacc tatctgctag
     5761 ataagtatca gttgaattct gataatactt attatatagg ggatcggact ctggatgtgg
     5821 aatttgccca gaatagtggg attcaaagca tcaacttttt agagtctact tatgaaggga
     5881 atcacaggat tcaagcgtta gcagatattt cccgtatttt tgagactaag tgataaaaag
     5941 attgtgtcag ttttgtgaca gagacctaac aaactatttc aagtaaccta gtttgttaca
     6001 aggaatagac agttctgtta aataggcccg agagggcttt ttttctacat tttttgtgtt
     6061 atgatagaca ggtactcatt tgaaaggaat ttgaaagaat gaagaaaaga atgttattag
     6121 cgtcaacagt aaccttgtca tttgccccag tattggcaac tcaagcagaa gaagttcttt
     6181 ggactgcacg tagtgttgag caaatccaaa acgatttgac taaaacggac aacaaaacaa
     6241 gttataccgt acagtatggt gatactttga gcaccattgc agaagccttg ggtgtagatg
     6301 taacagtgct tgcgaatctg aacaaaatca ctaatatgga cttgattttc ccagaaactg
     6361 ttttgacaac gactgtcaat gaagcagaag aagtaacaga agttgaaatc caaacacctc
     6421 aagcagactc tagtgaagaa gtgacaactg cgacagcaga tttgaccact aatcaagtga
     6481 ccgttgatga tcaaactgtt caggttgcag acctttctca accaattgca gaagttacaa
     6541 agacagtgat tgcttctgaa gaagtggcac catctacggg cacttctgtc ccagaggagc
     6601 aaacgaccga aacaactcgc ccagttgaag aagcaactcc tcaggaaacg actccagctg
     6661 agaagcagga aacacaagca agccctcaag ctgcatcagc agtggaagta actacaacaa
     6721 gttcagaagc aaaagaagta gcatcatcaa atggagctac agcagcagtt tctacttatc
     6781 aaccagaaga gacgaaaata atttcaacaa cttacgaggc tccagctgcg cccgattatg
     6841 ctggacttgc agtagcaaaa tctgaaaatg caggtcttca accacaaaca gctgccttta
     6901 aagaagaaat tgctaacttg tttggcatta catcctttag tggttatcgt ccaggagaca
     6961 gtggagatca cggaaaaggt ttggctatcg actttatggt accagaacgt tcagaattag
     7021 gggataagat tgcggaatat gctattcaaa atatggccag ccgtggcatt agttacatca
     7081 tctggaaaca acgttcctat gctccattcg atagcaaata tgggccagct aacacttgga
     7141 acccaatgcc agaccgtggt agtgtgacag aaaatcacta tgatcacgtt cacgtttcaa
     7201 tgaatggata aacccgactt gataacatca ttttgacgaa tgagatctag ctttcgtgat
     7261 ggaaagcgat tctcgttcgt tttttctttg tcatactctt cgaaaatctc ttcaaaccac
     7321 gtcagtttta tctgaaactt caaagctgtg ctttgagcaa cctgcgacta gcttcctagt
     7381 ttgctttttg attttcattg agtatcaatt tgaatggaaa atggaaagtt atcatcttgt
     7441 aatgagttaa gcaacattct tgcaatctat tttactttat atcacaatta attagtcaaa
     7501 tattgataaa tcaataaaaa gagaggggaa gaaatgctag agattcaaga tttactgtat
     7561 caactccgct tgtctgagca agcgagtacg caattgtttg aaaaaaggct tgggattagt
     7621 ttgacacggt atcagatttt actgtttttg ctggagcatt ctccttgtaa ccaaatggcg
     7681 gttcaggagc gtttgaaaat tgatcaggct gctttgacac ggcatttcaa aattttggaa
     7741 acggaaggtt tggtggagcg tcatcgtaat cctgaaaatc agcgggaagt gttggtagag
     7801 gctgcgaagt atgccaagga gcagttagtg gtgaatcccc ctctgcaaca tatcagggtt
     7861 aaggaagaga tagaaagtat cttaacagag tttgagagaa cagaactcag ccgtttatta
     7921 aataaattgg ttttgggtat tgaaaatata gaaatttaag gagaaataga tgtcaattat
     7981 tttaacaacg atcgttgctt tggagcattt ttacattttt tatttggaaa gtattgccac
     8041 gcaatcagat gcgactagtc gtgtatttaa tatggaaaag gaagaattgg ctcatccgtc
     8101 agtaagttca ttgttcaaaa atcaaggaat ttataaggct ctgctaggag tctttctctt
     8161 gtatgtcatt tatttctcac agaatttaga aattgtgact atttttgtct tatttgtgat
     8221 tggtgctgcg acttacggct ctttaacagc ggataaaaaa attattttga aacaaggtgg
     8281 atcagctatt ttggccttga ttagtatttt actctttaaa tacacttgaa ggtcgattct
     8341 aatctcgcta atccttttta atccagaata agggaaatat gttatacttg tttttaagaa
     8401 aaaagtctca ttgaattggt tttgaggagt tagaaatgaa agtattagtg acaggttttg
     8461 agccctttgg aggggaaaag ggcaatccag ctttggaggc cattaaaggt ttaccagctg
     8521 aaatccatgg tgctgaggtc cgttggctag aggtgccgac agtttttcac aaatctgctc
     8581 aagtattgga agaagagatg aatcgttatc aacctgactt tgtcctttgt attgggcaag
     8641 ctggtggaag aactagtttg acacctgaac gagtgaccat taatcaagac gatgcatgca
     8701 tttctgataa cgaagataat caaccgattg accgtcccat tcgcccagat ggtgcttcgg
     8761 cctactttag tagtttgccg attaaagcga tggttcaagc tataaaaaaa gagggcttac
     8821 cggcctctgt ttccaatacg gcagggactt ttgtctgcag ccatttgatg tatcaggctc
     8881 tctatttggt agaaaagaaa tctccatatg ttaaggcagg ttttatgcat attccttata
     8941 tgatggaaca ggtggtgaac agaccgacta ctccagctat gagtttagtg gatattcggc
     9001 gagggataga agcagcaatc ggcgctataa tagaacatgg agatcaggaa ctcaagttgg
     9061 taggcggaga aactcattga tagaaaaaag cttgagggga aaaaccttca agcttttgga
     9121 cgttttcggg ccaatactgc tcggtaaaac ataattttag tgcattggat ataaggtagg
     9181 agtgaaaaac tagcaatgcc aaaggtaatc caattgagga agtaccaagg aagaagctgt
     9241 aaatctagga caaagtgctg gaacttgtag cccttcataa aggaacggct agtttttagg
     9301 attcgtcttg gtgggacctg tcctaggtct agactataac agagaagaaa ttccacctgt
     9361 gaataggcat aatactgtgg aatatagagg atatttccta caatgatcaa gatgagactt
     9421 gcaagaaagt agagtccaaa gaccatgagg aaacgctcgg tttcaactga tgagagatct
     9481 agatttggaa actcaggatg tagggtgacg aattttttgg ctaaaaagct actataaaag
     9541 aggaggtaaa tcccaagtaa attagggata ctccataaaa agagatagaa acgtttgaga
     9601 agtagggtca aaaaggtttg agaaaagcgc tcctcatcaa agagagctag gctgtttttt
     9661 acagatggct ccgttttaga atctttcatg agtgtcagtg ttgcatagac ggaactggtc
     9721 aaaagaatag tcccgataaa ggagactagt agaggaaaga ggtaggtttg aagtatttgg
     9781 ccaagtatgc tgaaaaatgg ctgttctaaa acagtcccgt ggatccgaga taagggatta
     9841 agaaaaccag ataagatgac cagcatactg ggaaggatat agaggagaaa gagacggggg
     9901 gtgtcagcct gaaaatgttt tgactcctga cgaattgttt ttaaatcaat ttttggatag
     9961 ttcattctct tattatacca tagttcttat acatagttcg tgacagttcc tacttttttt
    10021 gataaaatca tacagtgtgt ccttgggcac actgtatgaa ctgggactgt ctttcccagc
    10081 ttcggaggta aaaaatgtca gattcaccaa tcaaatatcg tttgattaag aaagaaaaac
    10141 acacaggagc tcgtctggga gaaatcatca ctccccacgg tacctttccg acacctatgt
    10201 ttatgccagt tgggacacaa gccactgtca aaactcagtc acctgaagaa ttgaaggaga
    10261 tgggttcggg aattatccta tcaaatacct atcatctctg gcttcgccct ggagatgaac
    10321 tcattgcacg cgctggtggt ctccacaagt tcatgaattg ggaccagcct atcttgacag
    10381 atagtggtgg ttttcaggtt tattctttag cagatagccg taatatcaca gaagaaggag
    10441 taacctttaa aaatcatcta aatggttcta agatgttcct atccccagaa aaagccatct
    10501 ctattcagaa taatctgggt tcagacatca tgatgtcctt tgatgaatgt cctcagtttt
    10561 atcaacctta tgactacgtt aagaaatcga tcgagcgtac cagccgttgg gctgagcgtg
    10621 gtttgaaggc tcaccgtcgt ccacatgacc aaggtttgtt tggaattgtg caaggtgcag
    10681 gatttgaaga ccttcgccgc caatcagctc atgatcttgt cagcatggat ttctcaggct
    10741 actctatcgg tggtttggca gtgggagaaa cccatgaaga gatgaatgcg gtcttggact
    10801 ttacaactca actgctacct gaaaataaac ctcgttatct gatgggtgtg ggagcgccag
    10861 atagcttgat cgatggggtc attcgtgggg tggatatgtt tgactgtgtc ttaccgactc
    10921 gaattgctcg taacgggact tgtatgacca gtcaaggacg tttggttgtg aaaaatgccc
    10981 agtttgctga ggactttacg ccactggatc ctgagtgtga ctgctacaca tgtaataact
    11041 atacacgcgc ttaccttcgt cacctgctca aggctgatga aacctttggt atccgcttga
    11101 ctagctacca caatctttac ttcttgctta acctgatgaa gcaagtgcga caagccatca
    11161 tggatgacaa tctcttggaa ttccgtgagt attttgtgga aaaatatggc tataataagt
    11221 caggacgtaa tttctaaaat ggaattgata taaaaaaatc ctaagttttc tcttaggatt
    11281 tttcttcttt ttttgataga ataaagtgta caatgaaagg aagaataaac tcgtatgcgc
    11341 attaaatggt tttccttgat taggattata ggtttacttt tggtactctt gtatcacttc
    11401 tttcagacga tctttcctgg aggatttttc ggggtagatg tctttttcac attttcaggt
    11461 ttcctgatta cagctctact cattgaagaa ttttctaaga acaatgagat tgatttgata
    11521 ggatttttta gaagacgctt ttatcggatt gtgccacctg tggttttgat ggtcttggtg
    11581 accatgcctt ttactttctt ggttcgccaa gactatgttg ctggaattgg tggccagatt
    11641 gcgggcgtct taggctttat gaccaacttc tatgaactcc taacaggtgg gagttatgaa
    11701 tctcagttca ttcctcattt gtttgttcat aattggagtt tggcagttga ggttcactac
    11761 tatattcttt ggggattggc agtttggttc ttatccaaac aagctaaatc aaatggtcag
    11821 ttgaagggga tggtctttct cttatctgct gctgccttct tgatcagttt cttctccatg
    11881 tttattggta gttttctagt gacctcttat tcctctgttt atttctccag tttaactcat
    11941 gtctatccat tctttttggg aagtatgtta gcaactattg taggtgttcg tcagacgact
    12001 tccctcgtca agcagttgga taaaatctgg gatttacgaa agactttggt agtttttgga
    12061 ggaggctttg gtttcctagt tcttttgact ttctttgtca aattcactta tctttttgcc
    12121 tatcttatcg gcttcttgct tgccagtctt gcagctcttg ccatgattct ggcggcgcgt
    12181 gtcttacatg aaaagacaca tcatatacag gagccgaaga ttatcagctt tttagcggat
    12241 actagctatg cggtttatct tttccattgg cctttctata tcattttctc acagttgaca
    12301 tcaaatcttc ttgctgtatt actgactctg atttgttctt atggctttgc cagtctgtca
    12361 ttctatgtat tggaaccttg gattgcaggc aagaacacac ctattttaca aactcttcgt
    12421 cccctgcctt atattcacgc aattcttgta gcaggtacag gaatcttgac catcattgtc
    12481 tgcacggtga ccttgttggc accacaagtg ggagcgtttg agacagactt gactgtcaat
    12541 ggcttgaagc aagctgcaac aaatattggc cagaccaagg tgatggcaga acgggcagat
    12601 gcaaacagtt tgggaattgc tgatggcact atgttaattg gtgactcagt ggctttaagg
    12661 gcaaatacag cactacagac agctcttcct ggagcacaga ttaacgcgca ggtcagcgta
    12721 acaaccaaga ccgcaaatga aatcatgcta aataatagcc agaataaatt tttacctaag
    12781 acggtggtca ttgcgactgg ggtaaataat cctgagaatt acaaggatga ctgggacagt
    12841 atcgtgaaaa atcttcctaa gggacaccat atgattttgg tgactcctta tgagggagat
    12901 aagacaaaag agacctatgc catcgttgag aaggctgctg cctatatgag agaattggca
    12961 gagaagacac cttacattac gatagcagat tggaatcaag ttgcgaaaga gcatccagaa
    13021 atttgggctg gaacagacca ggttcatttc gggagtgaga gtagcactat cgaagcagga
    13081 gcaaaattgt atgcagatac gattgccaca gctttgcaga cagctcaaga caagccggtt
    13141 aaatcaaaat aacttgtatt aaaaaagaga agagttggag aaatccagct cttttaaaat
    13201 atctccaaaa aataggtcta taccatttac aaataaaaaa gaaaggttta taatgtaatt
    13261 gacataataa attgtagaat caatctttta aggaggttaa cattatgcct aactatatta
    13321 aagcggatca gtttttctac ccacacggag ttcgtcgagg tggttacttg gaacttgtgg
    13381 acggcaagtt tgggaaacat gtagagcaga ttcctgaagg ggctgaggtg attgactata
    13441 caggttatag cattgcccca ggtcttgtgg atactcatat tcatggatat gcaggtgtag
    13501 atgtgatgga caacaacatt gaaggtacat tgcatactat gagtgaagga cttcttagta
    13561 ccggtgttac cagtttctta cccacaactt taacagccac ttatgagcaa ttgcttgcag
    13621 tcactgaaaa tcttggaaac cattataaag aagcaacagg tgctaagatt cgtgggattt
    13681 attatgaagg tccatatttc acagaaactt ttaagggggc acaaaatcca acttatatga
    13741 gagacccggg tgttgaggag tttcattctt ggcaaaaagc ggcaaatggc ttgcttaata
    13801 aaattgccct tgcaccagaa cgtgatgggg tggaagactt tgttcgtaca gttacgggcg
    13861 aaggtgtgac ggttgctctt ggacattcaa acgcgacttt tgatgaagcc aaaaaagcag
    13921 tcgatgctgg agcgagtgtt tgggtgcatg cctacaatgg aatgcgtggg ttgactcacc
    13981 gtgaattggg tatggttgga gccatgtacc aattgccaca tacctatgca gagttgatct
    14041 gtgatggtca ccacgtagat ccaaaggctt gcgaaattct tatcaaacaa aaaggaacag
    14101 aaaacatcgc tcttatcaca gactgtatga cagctggggg attggaagac ggagattata
    14161 tgttgggaga attcccagtt gtcgttgcaa atggaactgc acgcctcaaa tcgacaggta
    14221 acttggcagg ttctatcctc aaactcaaag atggtttgaa gaatgtggtc gaatggggaa
    14281 ttgcgaatcc gcatgaagca gtcatgatgg ccagcttcaa cccagctaaa tccgttcaca
    14341 tcgatgacgt ctgtggccaa atccgtgaag gctacgacgc tgacttcatc gtattagata
    14401 aagatttgga attggtagca acctatctag atggcgtaaa acgttatcaa gcataagaga
    14461 gaaggcagaa gttagagact agcttctgct tttttataca gggtacttta ctggtaaata
    14521 aaaagttaaa aaaatcacaa aaaaagcttg aagaaacaaa gaaaaaggct tacaattgaa
    14581 ctataaatat tacaaataaa aaaacggagg agtgctttat gaaagcctat acttatgtta
    14641 aaccaggact tgcttctttt gttgatgtag acaaaccagt tattcgcaag ccaacagacg
    14701 ctattgtgcg tattgtaaaa accactattt gtggaacaga cctccatatt atcaaagggg
    14761 atgttcctac ttgccaaagt ggtaccattc ttggccacga agggattggg attgttgaag
    14821 aagttgggga aggagtttcc aacttcaaaa aaggcgacaa ggtcttgatt tcttgcgtct
    14881 gtgcctgtgg taaatgctac tactgtaaaa aaggaattta tgcccactgt gaagacgaag
    14941 ggggctggat tttcggtcac ttgattgatg gtatgcaggc tgaatatcta cgtgtccctc
    15001 atgcagataa tactctttac catactccag aagacttgtc agatgaagct ttggttatgc
    15061 tgtcagacat tctgcctact ggatatgaaa ttggtgtctt aaaagggaaa gtagaacctg
    15121 gttgcagcgt agccattatt ggttcaggtc cagttggatt ggctgctctt ttaacagccc
    15181 aattctattc accagctaaa ttgattatgg tagacctaga cgataaccgc ttggaaactg
    15241 ccctatcatt cggtgcgact cataaggtta attcttcaga ccctaaaaaa gccattaaag
    15301 aaatttatga tttgacagat ggtcgtggtg tggatgtcgc tatcgaagct gtagggattc
    15361 ctgaaacatt tgatttctgt caaaagatta tcggtgtaga cggaacggtt gccaactgtg
    15421 gtgtgcatgg taaaccagtt gaattcgatt tagataaact ttggattcgc aacatcaatg
    15481 taacaactgg tttggtatct acaaatacga ctccacaatt gttgaaagca cttgaaagtc
    15541 ataagattga accggaaaaa ttggtaactc actatttcaa actcagtgaa attgaaaaag
    15601 cctacgaagt cttcagtaag gcagcagacc accatgccat taaggtcatt atcgaaaacg
    15661 atatctcaga agcctaagta gtaaaaatat ttttgtacat aagtaaatag aaattcagtc
    15721 atccatcaga tggttggatt ttttatcaaa aaattaagaa atgagcatat ttctttcctt
    15781 gtctggcgga attggttata atatacggta caaaggaatg aatgaatatg tatcgtgtta
    15841 tagaaatgta cggagatttt gaaccgtggt ggttcttaga aggttgggaa gaagatattg
    15901 tagcaagtag aaaatttgac cagtattatg atgctctcaa atactacaaa acttgctggt
    15961 ttagattgga acaagaatcg cctctttata aaagtagaag cgacttgatg accatttttt
    16021 gggacccgga agaccaacgc tggtgtgatg aatgtgatga gtatttacaa caataccatt
    16081 ctttggctct tttgcaggat gagcaggtta tcccagacga aaaactacgc tcaggctatg
    16141 aaaaacaaac cagtcaggaa aggaatcgtt cttgccgtat gaaattaaaa tagagaaaag
    16201 taactttttg gagttgcttt ttttattttt ctaacgcttt gcgaatagta taggtgagga
    16261 ggtaagtatg gttcaagaaa ttgcacaaga aatcattcgt tcggctcgga aaaaaggggc
    16321 gcaagacatt tattttgtcc ccaagttaga tgcctatgag cttcatatga gggtaggaga
    16381 cgagcgctgt aaaattggta gctatgattt tgaaaagttt gcagccgtta tcagtcactt
    16441 taagtttgtg gcgggtatga atgtgggaga aaaaagacgt agtcaactgg gttcctgtga
    16501 ttatgcctat gaccagaaga tggcgtctct acgtttatct actgtaggcg attatcgggg
    16561 gcatgagagt ttggttatcc gtttgttgca cgatgaggag caggacttgc atttttggtt
    16621 tcaggatatt gaagaattag gcaagcagta caggcaacgg ggactctatc tttttgctgg
    16681 tccggttggg agtggtaaga cgaccttgat gcatgaattg tccaagtcac tctttaaagg
    16741 acagcaagtt atgtccatcg aagatcctgt cgaaatcaag caggacgaca tgcttcagtt
    16801 gcagttgaac gaagcaatcg gcctaaccta tgaaaatcta atcaaacttt ccttgcgtca
    16861 tcgaccagat ctcttgatta tcggagaaat tcgtgacagc gagacggcgc gtgcagtggt
    16921 cagagctagt ttgacaggtg cgacagtctt ttcaaccatt cacgccaaga gtatccgagg
    16981 tgtttatgag cgtctgctgg agttgggtgt gagtgaagaa gaattggcag ttgttctgca
    17041 aggagtctgc taccagagat taatcggggg aggaggaatc gttgactttg caaacagaga
    17101 ttatcaagaa caccaagcag ccaagtggaa tgagcaaatt gaccagcttc ttaaagatgg
    17161 acatatcaca agtcttcagg ctgagacgga aaaaattagc tacagctaag caaaaaaata
    17221 tcatcaccct atttaacaat ctcttttcta gcggttttca tctggtggag actatctcct
    17281 ttttagatag gagttccttg ttggacaagc agtgtgtgac ccagatgcgt acaggcttgt
    17341 ctcaggggaa atcattctca gaaatgatgg aaagtttggg atgttcaagt accattgtca
    17401 ctcagttatc tctagctgaa gttcatggca atcttcacct gagtttggga aagatagaag
    17461 aatatctaga caatctggcc aaggtcaaga aaaagttaat tgaagtagcg acctatcctt
    17521 tgattttgct gggttttctt ctcttaatta tgctggggct acggaattac ctgctcccac
    17581 aactggatag tagcaatatt gccacccgaa ttatcggtaa tctgccacaa atttttctag
    17641 gcatggtagg gcttgtttcc gtgcttgccc ttttagcact aactttttat aaaagaagtt
    17701 ctaagatgag tgtcttttct atcttagcac gccttccctt tattggaatc tttgtgcaga
    17761 cctacttgac agcctattat gcacgtgaat gggggaatat gatttcacag ggaatggagc
    17821 tgacgcagat ttttcaaatg atgcaggaac aaggttccca gctctttaaa gaaatcggtc
    17881 aagatctggc tcaaaccctg aaaaatggcc gtgaattttc tcagacgata ggaacctatc
    17941 ctttctttag gaaggaattg agtctcatca tagagtatgg ggaagttaag tccaagctgg
    18001 gtagtgagtt ggaaatctat gctgaaaaaa cttgggaagc cttttttacc cgagtcaacc
    18061 gcaccatgaa tttggtgcag ccactggttt ttatctttgt ggcactgatt atcgttttac
    18121 tttatgcggc aatgctcatg cccatgtatc aaaatatgga ggtaaatttt taaaatgaaa
    18181 aaaatgatga cattcttgaa aaaagctaag gttaaagctt ttacattggt ggagatgtta
    18241 gtagtcttgc tgattatcag cgtgcttttc ttgctctttg tacctaatct gaccaagcaa
    18301 aaagaagcag tcaatgacaa aggaaaagca gctgttgtta aggtggtgga aagccaggca
    18361 gaactttata gcttggataa aaatgaagat gctactctga gcaagttaca aggagatggg
    18421 cgcatcacgg aagaacaggt taaagcttat aatgaatact atactaaaaa tggaggggca
    18481 aatcgtaaag tcaatgatta aggcctttac catgctggaa agtctcttgg ttttgggact
    18541 tgtgagtatc cttgccttgg gcttatccag ctctgtccag tccacttttg cagcagtaga
    18601 ggagcagatt ttctttatgg agtttgaaga actctatcgg gaaacccaaa aacgcagtgt
    18661 agccagtcag caaaagacta gtctaaattt agatgggcag acgcttagca atggcagtca
    18721 aaagttgaca gttcctaaag gaattcaggc accatcaggc caaagtatta catttgaccg
    18781 agctgggggc aattcgtccc tggctaaggt tgaatttcag accagtaaag gagcgattcg
    18841 ctatcaatta tatctaggaa atggaaaaat taaacgcatt aaggaaacaa aaaattaggg
    18901 cagtgatttt actggaagca gtagtcgctc tagctatctt tgccagcatt gcgaccctcc
    18961 ttttgggaca aattcaaaaa aataggcaag aggaagcaaa aatcttgcaa aaggaagaag
    19021 tcttgagggt agctaagatg gccctgcaga cggggcaaaa tcaggtaagc atcaacggag
    19081 ttgagattca ggtattttct agtgaaaaag gattggaggt ctaccatggt tcagaacagt
    19141 tgttggcaat caaagagcca taaggtcaag gcttttacct tgttagaatc cctgcttgcc
    19201 ctcattgtca tcagtggggg attactcctt tttcaaacta tgagtcagct cctcatttca
    19261 gaagttcgct accagcaaca aagcgagcaa aaggagtggc tcttgtttgt ggaccaactt
    19321 gaggcagaat tagaccgttc gcagttcgaa aaagtagaag gcaatcgcct atacatgaag
    19381 caagatggca aggacatcgc catcggtaag tcaaagtcag acgattttcg taaaaccgat
    19441 gctagtggac gggggtatca gcctatggtt tatggcctca aatcagctca gattacagag
    19501 gacaatcaac tggttcattt tcgtttccag tttcaaaaag gcttagaaag ggagttcatc
    19561 tatcgtgtgg aaaaagaaga aagttaaggc aggtgttctc ctctacgcag tcaccatagc
    19621 agccatcttt agtcttttgt tgcaatttta tttgaaccga caaatcgccc actatcaaga
    19681 ctatgcttta aataaagaaa aattggttgc ttttgccatg gctaaacgaa ccaaggataa
    19741 agccgagcaa gaaagtgggg aacaggtttt taatctaggt caggtaagct atcaaaacaa
    19801 gaaaacaagc ttggtgacga ctgttcgtac gtctaagagc caatatgagt ttctgtttcc
    19861 ttcagtcaaa atcaaagaag agaaaagaga taaaaaggaa gaggtagcga ccgattcaag
    19921 cgaaaaagcg gagaaaaaaa atcagaagag aagcctgaaa agaaagagaa ttcctagtca
    19981 attcaactat aatacgttga atccagaata gtccactgta gtttctagaa aattgctgga
    20041 aatggatgtt aagctccaat tcatttgttt atatcttatt tcagtttact atactttgtg
    20101 ctaaactaaa gatatgaaac atgattttaa ccacaaagca gaaactttcg actcccctaa
    20161 aaatatcttc ctcgcaaact tggtatgtca agcagtcgag aaacagattg atattttatc
    20221 agacaaagta attttagatt tcggtggtgg cacgggtcta ttagccttgc ccctagccaa
    20281 gcaggctaag tcagttactc ttgtagacat ttctgagaaa atgttggagc aagctcgttt
    20341 gaaagtggag cagcaagcaa tcaagaatat ccagtttttg gagcaagatt taccgaaaaa
    20401 tcccttggag aaagagtttg attgccttgc tgttagtcgg gttcttcatc atatgcctga
    20461 tttggatgcg gctctctcac tgtttcatca acatttgaag gaagatggga aactcatcat
    20521 tgctgatttt accaagacag aagctaatca tcatggattt gatttagctg aactggaaaa
    20581 caagctaatt gagcatggtt tttcatctgt gcatagtcag attctctata gcgctgaaga
    20641 cctgtttcaa ggaaatcact cagaattctt tttaatagta gcccaaaaat cactcgccta
    20701 gtcagggagt gatttttcta taaggatgga aaaaagaagg gaaatttgat aagataggaa
    20761 tatggatttt gaaaaaattg aacaagctta tacctattta ctagagaatg tccaagttat
    20821 ccaaagtgat ttggcgacca acttttatga cgccttggtg gagcaaaaca gcatctatct
    20881 ggatggtgaa actgagctaa accaggtcaa agacaacaat caggccctta agcgtttagc
    20941 actacgcaaa gaagaatggc tcaagaccta ccagtttctc ttgatgaagg ctgggcaaac
    21001 agaacccttg caggccaatc accagtttac accagatgcc attgctttgc ttttggtgtt
    21061 tattgtggaa gagttgttta aagaggagga aattactatc ctcgaaatgg gttctgggat
    21121 gggaattcta ggcgctactt tcttgacctc gcttgataaa aaggtggatt acttgggaat
    21181 ggaaatggat gatttgctga ttgatctggc agctagcatg gcagatgtaa ttggtttgca
    21241 ggctggcttt gtccaaggag atgccgttcg tccacaaatg ctcaaagaaa gcgatgtggt
    21301 catcagtgac ttgcctgttg gctattatcc tgatgatgcc gttgcgtcgc gccatcaagt
    21361 tgcttctagc caagaacata cttacgccca tcacttgctc atggaacaag ggcttaagta
    21421 cctcaagtca gacggatacg ctatttttct agctccgagt aatttgttga ccagtcctca
    21481 aagtgatttg ttaaaagaat ggctgaaaga agaggcgagt ctggttgcta tgattagtct
    21541 gcctgaaaat ctctttgcta atgccaaaca atctaagact atttttatct tacagaagaa
    21601 aaatgaaata gcagtagagc cttttgttta tccacttgct agcttgcaag atgcaagtgt
    21661 tttaatgaaa tttaaagaaa attttcaaaa atggactcaa ggtactgaaa tataaaatag
    21721 attttgttat aatagttgaa aacgcttaaa aaggggtatc atgttatgac aaaaacaatt
    21781 gcaatcaatg caggaagttc aagtttgaaa tggcaattat acttaatgcc agaagaaaaa
    21841 gtgttagcca aaggtttgat tgaacgtatc ggtttgaaag attcaatttc aactgtaaaa
    21901 tttgacggcc gttctgagca acaaattttg gatattgaaa atcatacaca agccgttaaa
    21961 attttattgg atgacttgat tcgtttcgat attatcaagg cttatgacga gattacaggt
    22021 gttggacatc gtgttgttgc tggtggagaa tatttcaaag aatcaacagt tgttgaggga
    22081 gatgttttag aaaaagttga agagttgagt ttgttggctc ctctacacaa cccggccaat
    22141 gcagcaggtg ttcgtgcctt caaggaattg ttgccagaca ttaccagtgt agttgttttt
    22201 gatacttcct tccacacaag tatgccagag aaagcttatc gctaccctct accaacaaaa
    22261 tattacacag aaaacaaggt tcgtaaatac ggtgctcatg gtacaagtca ccagtttgta
    22321 gcaggagaag ctgcaaaact cttgggacgt ccattagaag acttgaagtt aattacctgt
    22381 catattggta acggaggttc aattacagct gtgaaagccg gcaaatctgt agacacttct
    22441 atggggttca ctcctcttgg tggtattatg atgggaacgc gtacagggga tattgatcca
    22501 gctatcattc cttatttaat gcaatataca gaggatttta acacaccaga agatatcagt
    22561 cgtgttctta accgtgaatc aggtcttttg ggagtttctg ctaattctag cgatatgcgc
    22621 gatatagaag cagctgtagc agaagggaat cacgaggcta gcttggctta tgaaatgtat
    22681 gttgaccgta tccaaaaaca tatcggtcag taccttgcag tgctaaatgg agcagatgcc
    22741 attgttttca cagcaggtgt cggtgaaaat gcagagaatt tccgtcgtga tgtaatctca
    22801 gggatttcgt ggtttggttg tgatgttgat gatgaaaaga atgtctttgg cgttacagga
    22861 gacatctcaa cagaggcagc taaaatccgt gtcttggtta ttccaacaga tgaagaatta
    22921 gtcattgccc gtgacgttga acgcttgaaa aaataagtga aactaaaaaa atattcaata
    22981 caaggagttg ggaaagttat ttttccagct tctttttctg atgaaattgt ccaaaacctt
    23041 gctatgattg gcttttttga aaaatatggt ataatagtag taatttaata gatggagttg
    23101 agttttgaag aaaaactttc gtgtaaaaag agagaaagat tttaaggcga ttttcaagga
    23161 ggggacaagt tttgctaatc gcaaatttgt ggtctaccaa ttagaaaacc agaaaaacca
    23221 ttttcgagta ggtctatcag ttagcaaaaa actggggaat gccgtcacta gaaatcaaat
    23281 taagcgacgg attcggcata ttatccagaa tgcaaaaggg agtctggtag aagatgtcga
    23341 ctttgttgtc attgctcgaa aaggagtcga aaccttggga tacgcagaga tggagaaaaa
    23401 tctactccat gtattaaaat tatcaaagat ttaccgggaa ggaaatggga gtgaaaaaga
    23461 aactaaagtt aactagtttg ctaggactgt ctctgttaat catgacagcc tgtgcgacta
    23521 atggggtaac tagcgatatt acagccgaat cggctgattt ttggagtaaa ttggtttact
    23581 tctttgcgga aatcattcgc tttttatcgt ttgatattag tatcggagtg gggattattc
    23641 tctttacggt cttgattcgt acagtcctct tgccagtctt tcaggtgcaa atggtggctt
    23701 ctaggaaaat gcaggaagct cagccacgca ttaaggcgct tcgagaacaa tatccaggtc
    23761 gagatatgga aagcagaacc aaactagagc aggaaatgcg taaagtattt aaagaaatgg
    23821 gtgtcagaca gtcagactct ctttggccga ttttgattca gatgccggtt attttggccc
    23881 tgttccaagc cctatcaaga gttgactttt taaagacagg tcatttctta tggattaacc
    23941 ttggtagtgt ggatacaacc cttgttcttc cgattttagc agcagtattc acctttttaa
    24001 gtacttggtt gtccaacaaa gctttgtctg agcgaaatgg cgctacgact gcgatgatgt
    24061 atgggattcc agtcttgatt tttatctttg cagtttatgc gccaggtgga gtcgccctat
    24121 actggacagt gtctaatgct tatcaagtct tgcaaaccta tttcttgaat aatccattca
    24181 agattatcgc agagcgcgag gccgtagtac aggcacaaaa agatttggaa aatagaaaaa
    24241 gaaaagccaa gaaaaaggct cagaaaacga aataaataag gaggaatctg gtaatggtag
    24301 tatttacagg ttcaactgtt gaagaagcaa tccagaaagg attgaaagaa ttagatattc
    24361 caagaatgaa ggctcatatc aaagtcattt ctagggagaa aaaaggcttt cttggtctat
    24421 ttggtaaaaa accagcccaa gtggatattg aagcgattag tgaaacgact gttgtcaaag
    24481 caaatcaaca ggtagtaaaa ggcgttccga aaaaaatcaa tgatttgaac gagcctgtga
    24541 agacggttag tgaagaaacc gttgaccttg gtcatgtggt taatgctatt aaaaaaatag
    24601 aggaagaagg tcaaggtatt tctgatgaag tcaaggctga aatcttaaaa catgaaagac
    24661 atgccagcac tatcttagaa gaaactggtc acattgagat tttaaatgaa cttcaaatcg
    24721 aggaagcgat gagggaagaa gcaggcgctg atgaccttga aactgagcaa gatcaaactg
    24781 aaaatcaaga cttgaaagag atgggcttga aggtcgagca aagttatgat attgcccagg
    24841 tggctacgga tgtgactgcc tatgttcaag cgattgtgga tgacatggat gttgaggcta
    24901 cactttcaaa tgattataac cgtcgtagca tcaatctaca aattgacacc aacgaaccag
    24961 gtcgtattat cggctaccat ggtaaagtct tgaaggcctt gcaactgttg gctcaaaatt
    25021 atctttacaa ccgctattcc aaaaccttct acgttacaat caatgtcaat gattatgtcg
    25081 aacaccgtgc agaagtcttg cagacctatg cgcaaaaatt ggcgaatcgt gttttggaag
    25141 aaggtcgcag tcataaaaca gatccaatgt caaatagcga acgcaagatt atccatcgta
    25201 ttatttcacg tatggatggc gtgactagtt actctgaagg tgatgagcca aatcgctatg
    25261 ttgttgtaga tacagaataa gtaaaatcag ggttgacctg attttttgct agttagagga
    25321 ggttaaactg atgttgaata agataagaga ctatctagac ttttctggtt tgcagtaccg
    25381 tagtcctgat aaagcgggag cagagcgaga gaagatgctg gcgttccgcc acaaaggaca
    25441 agaggcccga aaggctttta cagaactggc caaagccttt caagcaagcc atccagaatg
    25501 gcaactccaa cagactagtc agtggatgaa tcaggcccag cgtttgagac cacatttttg
    25561 ggtttatcta cagagagacg gacaagtgac agaacctatg atggccttac gtttgtatgg
    25621 gacatctact gactttggaa tttctttgga agtcagtttc atcgaacgta agaaggatga
    25681 gcaaacactg ggcaagcagg ccaaagtttt agacattcca accgttaaag ggatttatta
    25741 tctaacctac tctaatggtc aaagtcaacg gtgggaggcg aatgaagaaa agcgtcgtac
    25801 tttacgcgag aaggtgagaa gtcaagaagt tcgaaaagtt ttagtgaagg tagatgttcc
    25861 tatgacagaa aattcgtctg aagaagaaat cgtagaaggc ttattgaagt cttattctaa
    25921 aattcttccc tattatctag ctacgagaaa ataagataat ttgtaaaaca tcataaatca
    25981 tacagtccaa gagtgaacag tccgctgtgt aattcttggt ctttttgttt gcgctttcgc
    26041 attatataat aaacttacaa aaacaattca aaaggagaac aattatggaa gtcgtttcaa
    26101 gtgttctaaa ttggttttct agcaatattt tgcagaatcc cgcatttttc gtaggtttat
    26161 tggtgttgat aggatatgca cttttgaaaa aacctgccca tgacgttttt tcagggtttg
    26221 ttaaagcaac agtagggtat atgttgctta acgtgggtgc tggtggtttg gttacaacct
    26281 ttcgtccaat cttagcagct cttaactaca aattccaaat tggtgcagcg gttatcgacc
    26341 cttattttgg acttgctgca gcaaacaaca aaattgtagc agagtttcca gattttgttg
    26401 gaactgcaac tacagctcta ttgattggtt ttggaataaa tatcttgctc gtagctcttc
    26461 gaaagattac gaaggtaaga accctcttta ttactggtca catcatggta caacaagctg
    26521 caacagtatc tcttatggtt ctattcttag taccacaatt gcgcaatgct tacggtacag
    26581 cagcgattgg tatcatctgt ggactttact gggcagttag ttcaaatatg actgttgagg
    26641 caactcaacg cttgactggt ggtggcggat ttgcgattgg tcaccaacag caatttgcaa
    26701 tctggtttgt agataaagta gcaggacgct ttggtaagaa agaagaaagt ttagacaatc
    26761 ttaaattacc taagttcctc tcaatcttcc acgatacagt tgttgcatct gctaccttga
    26821 tgctcgtatt cttcggagcc attcttttaa tcttgggtcc agacattatg tctaataaag
    26881 aagtcatcac ttcaggaact ctattcaatc ctgctaaaca agatttcttt atgtacatta
    26941 tccaaacagc ctttaccttc tcagtttact tgttcgtttt gatgcaaggt gtccgaatgt
    27001 tcgtatctga gttgacaaac gccttccaag gtatttcaaa caaattgttg ccaggttcat
    27061 tcccagcggt tgacgttgca gcttcttatg gatttggttc tccaaatgct gtcttgtcag
    27121 gatttacctt tggtttgatt ggtcaattga ttacaattgt cttgctcatc gtctttaaaa
    27181 atccgattct tattattaca ggatttgtac cagtgttctt tgacaatgca gccattgcgg
    27241 tctacgctga taaacgcggc ggatggaaag cggctgttat cctttccttt atatcaggtg
    27301 tccttcaagt tgctctagga gctctttgtg tggcccttct cgatttggca gcttatggtg
    27361 gctaccatgg aaatatcgac tttgaattcc catggcttgg atttggatat atcttcaaat
    27421 accttggtat tgttggttat gtacttgtgt gtctcttctt gcttgttatt cctcaacttc
    27481 aatttgccaa agcaaaagat aaagagaaat attacaacgg tgaagttcaa gaagaagctt
    27541 agtatctaga aaaggagaaa taaaatggtt aaagtattag cagcgtgcgg aaatggaatg
    27601 ggttcatcaa tggttatcaa gatgaaggtt gaaaatgctc tccgtaagct taatcaaaga
    27661 gattttacag tcaattcatg cagtgtcggt gaagctaaag gtttagcagt aggatatgac
    27721 atcgtaatcg cttctcttca tttgattcaa gaattggaag ggcgaactaa tgggaagtta
    27781 attgggcttg ataacttgat ggatgataaa gaaatcaccg aaaaactcag tcaagcacta
    27841 cagtaaaagg ttggaggggg ctggacagaa actgagagtt atcgtttctg tccttctccc
    27901 tcttaaataa aggaggcaga tatgaattta aaacaagctt taattgacaa tgactcgatc
    27961 cgactaggtt tagaggctaa caattggaaa gaagcagtca aggtagcagt agatccctta
    28021 attgaaagtg gggcaatttt gccagagtat tacgatgcta tcattgaatc gactgaagag
    28081 tatgggcctt actatatctt gatgccaggt atggctatgc cccacgctag acctgaagct
    28141 ggtgtgcaaa gtgatgcctt ttcattgatt accttacaaa atcctgttgt attttcagat
    28201 gggaaagagg tatctgtttt gttggcacta gcagcaacaa gctcaaaaat tcacacaagt
    28261 gtagccattc cacaaattat tgccctgttt gaattagaag attctattgc acgtttacag
    28321 gcttgccaga ctaaagaaga tgtcttggct atgattgaag aatctaagga tagcccttat
    28381 ctcgaaggat tggatttgga aagttagaaa gaggaataaa gaaatgacaa aaagaatacc
    28441 taatttacaa gttgcattag accattcaga cttgcaagga gcgattaaag cagctgtttc
    28501 tgttggtcag gaagtagata ttatcgaagc tggaactgtt tgcttgcttc aagttggaag
    28561 tgaactggct gaagtcttgc gtagcctttt cccagataag attattgtgg cagacacaaa
    28621 atgtgctgat gctggtggaa cagttgctaa aaataatgcg gttcgtggag cagactggat
    28681 gacttgtatc tgttgtgcaa ccatccctac tatggaagca gctctaaagg ctatcaagac
    28741 tgaacgagga gaacgaggcg aaatccagat cgagctttat ggcgattgga cttttgaaca
    28801 agctcagctt tggctagatg caggtatctc acaagctatt tatcaccaat ctcgtgatgc
    28861 tcttcttgct ggtgaaactt ggggtgaaaa agaccttaat aaggttaaaa aactcattga
    28921 catgggcttc cgtgtatctg taacaggtgg tctagatgta gatactctca aactctttga
    28981 aggtgttgat gtctttacct ttatcgcagg tcgtggaatt acagaggctg cggatccagc
    29041 aggagcagcg cgtgccttca aggatgaaat caaacgaatt tgggggtaaa tcatggtacg
    29101 tccaattgga atttatgaaa aggcaacccc aacacacttt acttggctag aacgtttaaa
    29161 ttttgccaag gagttaggct ttgattttgt cgagatgtct attgacgaac gtgacgagcg
    29221 tttagcaaga cttgactgga gtaaggaaga acgcttggaa gttgtcaaag caatctatga
    29281 aactggtgtt cgtattcctt ctatctgttt ttcaggccat cgtcgctacc cattgggttc
    29341 aaaagatcca gttctagagg aaaaatctct agaactcatg aaaaaatgta tcgaattagc
    29401 tcaagacttg ggagttcgta cgattcaatt agctggttac gatgtttact atgaggaaaa
    29461 gtcaccccag acacgccaac gttttatcaa aaatttgaga aaagcctgtg actgggctga
    29521 agaagctcag gtggtacttg ctattgaaat tatggatgat cctttcatca gtagcatcga
    29581 aaaatatttg gctatagaaa aagagattga ctctcccttc ctctttgtat atccagatat
    29641 tggtaatgtg tctgcatggc ataatgatat ctatagtgag ttttatcttg gtcatcatgc
    29701 catcgcagct ctccatctca aggatactta tgcagtgaca gaaagttcaa ggggccagtt
    29761 ccgagatgta ccttttgggc aaggttgtgt caaatgggaa gaagctttcg atattttaaa
    29821 ggaaaccaat tataatggac ctttcctaat cgaaatgtgg tctgaaaatt gtgaaacagt
    29881 agaagaaaca cgcgcagcca ttcaagaggc gcaagctttt ctctatccac tcattaagaa
    29941 agcaggtttg atgtaagatg aatcaagtaa tcaatgctat gcgtaaacga gtctgtgatg
    30001 ccaatcaatc attgccaaaa catggacttg tcaaatttac ctgggggaat gtatctgaag
    30061 tcaatcgcga actcggtgtc attgttatca aaccatcagg cgtggattat gacgaattga
    30121 cacctgaaaa catggtagtg actgatctag atggtaagat cctagaaggg gatttaagac
    30181 catcttccga cctcccaact catgtgcaat tatatagggc ttggtcagaa attggtagtg
    30241 tggttcacac ccattcgaca gaagctgttg gttgggctca ggcaggtcgt gatattcctt
    30301 tctacggaac aacccatgca gattatttct acggttcaat cccttgcgcc cgtagtttga
    30361 ccaaggacga agtagaagtg gcctatgaaa aagatactgg cctggttatc gtagaagaat
    30421 ttgaacatcg cggacttaac ccggttgaag taccaggaat tgttgtacgc aatcacggtc
    30481 cattcacctg gggcaaaaat ccagagaatg ctgtttatca ctctgtcgta ctagaggaag
    30541 tatcaaagat gaatcgcttt acagaacaaa tcaatccaag agttggacct gctccccagt
    30601 acatactaga aaaacactac caacgtaaac atggaccaaa tgcttattat ggtcaaaagt
    30661 aagaacgatg aaggaggaga aaaagataaa tttagctcct ctttttacat ttgattttta
    30721 ttgagagtaa agttggagtt gaagtaattt tgaaagattt tttagaaata gcgcttgata
    30781 tatatatggt aaaataaaaa gaattgctgt gatatcaata gatttggggg attttttaat
    30841 atggtactgg ataaggcaag ttgtgattta cttcaatatt tgatggatca agaaacgtcc
    30901 aaaacgatta tggcgatttc gaaagatttg aaagagtcaa gaaggaaaat ttattatcac
    30961 attgacaaaa tcaatgctgc tctgggtgac gaggcgcttc acatcattag tattccacga
    31021 attggtattc acttaacgga agagcagaga gatgcttgtt gtaaactatt atcggaagta
    31081 gattcgtacg attatatcat gagtgcacat gaacgtatga tgataatgtt actatggata
    31141 ggtatttcta aagaacgtat tacgattgaa aaattgatag agttaacaga ggtatctagg
    31201 aatactgttc tcaatgattt gaatagtatt cgttatcaac taactttgga acaatatcag
    31261 gtgatcttgc aagtgagcaa gtcacaggga taccaccttc atgcccaccc tcttaataaa
    31321 attcagtatc ttcaatcgct tctatatcat atttttatgg aagaaaatgc cacttttgta
    31381 tctattttag aagataagat gaaagagagg ttagatgatg agtgtttgct ttctgttgaa
    31441 atgaaccaat tttttaagga acaggttcct ttagttgaac aagatttagg gaagaaaata
    31501 aaccatcatg aaataacttt tatgttgcag gttctacctt atttgctgtt aagctgtcat
    31561 aatgttgaac agtatcaaga aagacatcag gatatagagc aagaattttc tttgataaga
    31621 aaaagaatag agtatcaggt gtctaagaag ttaggagaac ggttgtttca aaagtttgaa
    31681 atttctttgt caggacttga agtttctctt gtagctgttc tcctcctctc ctatcgtaaa
    31741 gatttggata ttcatgcaga aagtgatgat tttcggcaat taaaacttgc tttagaagaa
    31801 tttatctggt attttgaatc acaaatccga atggagattg agaacaagga tgatttgtta
    31861 cgaaatttga tgatccactg taaagccttg ttatttagaa agacttacgg tattttttct
    31921 aaaaatcctc taacaaaaca aattcgatcc aagtatggag aattattttt agtcactaga
    31981 aaatctgcgg aaattttaga aggagcatgg tttattcggc taacagacga tgatattgcc
    32041 tatttgacga ttcatattgg aggattttta aaatatacac catcgtctca aaaaaatatg
    32101 aaaaaagttt atctcgtttg tgatgaaggt gttgcggttt cgagactttt gctgaaacaa
    32161 tgcaaacttt attttccaaa tgagcaaatt gacactgtat ttacaacaga acaatttaag
    32221 agtgtggaag atattgcaca agttgatgta gtgattacta ctaatgatga tttggatagc
    32281 agatttccga ttttaagggt taatcctatc cttgaagcag aagatatttt gaaaatgcta
    32341 gactatctta aacacaatat atttcgtaat aagagcaaaa gtttcagtga aaatctttct
    32401 agtcttattt cgtcttatat tgtagacagc aagttggcta gtaagttcca agaagaggtt
    32461 caaacactta taaatcaaga aatagtagtt caagcttttt tggaagatat ttgaaggaca
    32521 gtccaatgat gaacacaaac ctgtgttttt cttggtcttt tttagtgttt tgaagggtgg
    32581 tatactaatc tcaaagataa caattatatc caaaggaggc aacatatgcc aaacgtcaaa
    32641 gaaattacaa gagagtcatg gattttagcc actttcccag agtggggaac atggttgaac
    32701 gaagaaatcg aagaagaagt cgtacctgaa ggcaactttg ccatgtggtg gctaggcaac
    32761 tgtggtactt ggattaagac accagctggt gctaacgttg tcatggacct ttggtcaaac
    32821 cgtggaaaat caaccaaaaa agtgaaagat atggttcgtg ggcaccaaat ggcaaatatg
    32881 gcaggtgttc gtaagttgca accaaacttg cgtgttcagc caatggttat cgatccattt
    32941 gctatcaacg aactagacta ttacttagtt tcacacttcc acagtgatca tatcgaccca
    33001 tacacagctg cagcaattct caacaatcct aagttagagc atgttaagtt tatcggtcct
    33061 taccactgtg gacgaatctg ggaaggatgg ggtgttccaa aagaacgtat catcgttgtt
    33121 aaaccaggtg acactatcga attaaaagat atgaagattc atgcagtaga atcatttgac
    33181 cgtacttgct tggtaactct cccagtgaac ggtgctgatg agacaggcgg tgaacttgct
    33241 ggcttggctg ttacagatga agaaatggct caaaaggctg ttaactatat ctttgaaaca
    33301 ccaggtggaa ccatctatca tggtgcagat tctcacttct caaactattt tgcaaaacat
    33361 ggtaaagact ttaaaattga tgttgctttg aataactatg gtgaaaatcc ggtaggtatc
    33421 caagacaaaa tgacatctat cgaccttctt cgtatggcag aaaatctgcg taccaaagtc
    33481 attatcccag ttcactatga tatctggtct aacttcatgg cttctactaa tgagattcta
    33541 gaactttgga aaatgcgaaa agatcgcttg caatacgatt tccatccatt tatctgggaa
    33601 gttggcggta agtacactta tcctcaagat caacacttag tagaatacca tcatccacgt
    33661 ggttttgatg attgttttga acaagactct aacattcaat ttaaagcttt gctataatat
    33721 aaaaatattc tttggaggtt atccgtatgt tgcatgatac ggataatttt catataatag
    33781 taaaatagaa tgtgtgattc aataatcacc tcaaatagaa aggaaattct atgtcaaatc
    33841 tatctgttaa tgcaattcgt tttctaggta ttgacgccat taataaagcc aactcaggtc
    33901 atccaggtgt ggttatggga gcggctccga tggcttacag cctctttaca aaacaacttc
    33961 atatcaatcc agctcaacca aactggatta accgcgaccg ctttattctt tcagcaggtc
    34021 atggttcaat gctcctttat gctcttcttc acctttccgg ttttgaagat gtcagcatgg
    34081 atgagattaa gagtttccgt caatggggtt caaaaacacc aggtcaccca gaatttggtc
    34141 atacggcagg gattgatgct acgacaggtc ctctagggca agggatttca actgctactg
    34201 gttttgccca agcagaacgt ttcttggcag ccaaatataa ccgtgaaggc tacaatatct
    34261 ttgaccacta tacttacgtt atctgtggag acggagactt gatggaaggt gtctcaagcg
    34321 aggcagcttc atacgcaggt ttgcaaaaac ttgataagtt ggttgttctt tatgattcaa
    34381 atgatatcaa cttggatggt gagacaaagg attcctttac agaaagtgtt cgtgaccgtt
    34441 acaatgccta cggttggcat actgccttgg ttgaaaatgg aacagacttg gaagccatcc
    34501 atgctgctat cgaaacagca aaagcttcag gcaagccatc tttgattgaa gtgaagacgg
    34561 ttattggata cggttctcca aacaaacaag gaactaatgc tgtacacggc gcccctcttg
    34621 gagcagatga aactgcatca actcgtcaag ccctcggttg ggactacgaa ccatttgaaa
    34681 ttccagaaca agtatatgct gatttcaaag aacatgttgc agaccgtggc gcatcagctt
    34741 atcaagcttg gactaaatta gttgcagatt ataaagaagc tcatccagaa ctggctgcag
    34801 aagtagaagc catcatcgac ggacgtgatc cagtcgaagt gactccagca gacttcccag
    34861 ctttagaaaa tggtttttct caagcaactc gtaattcaag tcaagatgcc ttgaatgttg
    34921 tggcagctaa attaccaacc ttcctaggtg gatcagctga ccttgctcac tcaaacatga
    34981 cttatatcaa gacggatgga cttcaagacg atgccaatcg cttgaatcgt aacattcagt
    35041 ttggtgttcg tgaatttgca atgggaacaa tcttgaacgg gatggccctt catggtggac
    35101 ttcgtgtata cggtggaact ttcttcgtct tctctgacta tgtgaaggca gctgtccgct
    35161 tgtcagcctt acaaggactt cctgtgactt atgtctttac ccatgattca atcgcagttg
    35221 gggaagatgg tccgactcac gaaccagttg agcatttagc aggtcttcgt gctatgccaa
    35281 atctaaatgt tttccgtcca gcagatgcgc gtgaaacgca agcagcttgg taccttgcag
    35341 tgacaagtga gaaaacacca actgcccttg tcttgacacg tcaaaacttg actgttgaag
    35401 atggaacaga cttcgacaag gttgctaaag gtgcttatgt tgtatatgaa aatgcagccg
    35461 actttgatac catcttgatt gcgacaggtt cagaggttaa tcttgctgtc tcagctgcta
    35521 aagaattggc tagtcaaggc gaaaaaatcc gcgtagtcag catgccatct acagatgtct
    35581 ttgataaaca agatgcagct tacaaggaag aaattcttcc aaatgcagtc cgccgtcgtg
    35641 ttgcagtcga aatgggtgca agtcaaaact ggtacaaata tgttggtctc gatggtgccg
    35701 ttctaggtat tgataccttc ggagcctctg ccccagcacc aaaagtattg gcagaatatg
    35761 gctttactgt cgaaaatctt gtaaaagttg ttcgaaattt gaaataatcc taaaaatcag
    35821 ggcgtaagct ctggtttttc ttaccagaaa agtaaggtac aatcttgtaa aagaagctga
    35881 aatttgatat agtagtccta tgtaaaagac aaaggagaaa acaatgaatc caaatattac
    35941 ttttttaatc atgcttgtag gtatgatggc cttgatgttc tttatgcaac gttctcaaaa
    36001 gaaacaagct caaaagcgta tggaaagctt aaataaacta caaaaaggct atgaagtgat
    36061 tacaatcggt ggactttatg gaacagtcga tgaagtagat acggagaagg gaacaatagt
    36121 tcttgacgta gatggagttt acttgacttt tgaactagct gctatcaaga cagtattacc
    36181 gctgaaagaa acagcttcac tagaaggcgc aattgaaaaa taagacggga tcacgaactc
    36241 ccgttttcta taaaagaaag gaaatgggat gaaaaaatta gtctttgtct gtctgggaaa
    36301 tatttgccgt agccctatgg ccgagtttgt tatgaaatca atgacagata actacgaaat
    36361 ccaaagtcga gcaacttcct cttgggaaca tggcaatccg attcataagg ggactcaggg
    36421 aatttttcaa gagtatgaga ttccttatga caagaacaag acatcgcttc agattagtaa
    36481 ggaagatttt gaagcctttg attatattat cggaatggac gcttcaaatg tttccgactt
    36541 acgtcagatg tgtccagtag actgtcaaga taagatttac tcattttcat ctgaaagtgt
    36601 tccagaccct tggtatacag gagattttga agaaacctat cgacgtgttc aagagggctg
    36661 tcaagcttgg ttagaacgtt tagaaaagga gagttaaggt ggaaaatctt acgaattttt
    36721 acgaaaagta tcgtgtttat ctgactcgtc cacgtttaga gattttgaca gtagttacca
    36781 ttgttttctg tgctgtactc gtcttttttc taaatattcc aggaaaaggt gtcttaaaac
    36841 tcgataatgg aacgattgtt tatgatggca gtcttgtccg cggtaaaatg aatggccaag
    36901 gtaccattac cttccaaaat ggagaccaat atacaggtgg cttcaacaat ggagccttca
    36961 acggaaaagg tacctttcaa tctaaagaag gctggaccta cgaaggtgat tttgtaaatg
    37021 gtcaggctga aggaaaaggg aaactaacaa cagaacaaga agtcgtttat gaaggaactt
    37081 ttaaacaagg cgtttttcaa caaaaataaa gcctccttat caaaggaggt attattagaa
    37141 ttacaaggta agcgcttacc tgtaaatccc tttctttcca aatccctctt ccaagcaagt
    37201 ttgtgaaata aaaaatattt gaaataaatt tcacaaactt taaagataaa acctgataag
    37261 aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc tttgtgaaat gtttatgaaa
    37321 ctgtttacaa agttgaaata atgcgttata ataaacttgt gaaaaaatta acaaaggata
    37381 tcgatccttg aaagctatgg aggaaaatat ggctgataaa aaaactgtga caccagagga
    37441 aaagaaactc gttgctgaaa aacacgtaga tgagttggtt caaaaagctc tagttgccct
    37501 tgaagaaatg cgtaaattgg atcaagagca agttgactac atcgttgcca aagcatcagt
    37561 agcagctttg gatgcccacg gagaattggc tttacatgcc tttgaagaaa caggacgtgg
    37621 tgtatttgaa gataaagcaa ctaagaactt gtttgcttgt gaacacgtag taaacaacat
    37681 gcgccacact aagacagttg gcgttatcga agaagacgat gtaacaggat tgactcttat
    37741 tgctgaacca gttggtgttg tttgtggtat tactccaaca acaaacccaa catcaacagc
    37801 aatcttcaaa tcattgattt cattgaagac acgtaaccca atcgtctttg ccttccatcc
    37861 atcagcacaa gaatcatctg ctcatgcagc tcgtatcgtc cgcgatgcag ctatcgcagc
    37921 tggtgctcct gaaaactgtg tgcaatggat tactcaacca tctatggaag caacaagtgc
    37981 ccttatgaac cacgaaggtg ttgcgacaat ccttgcaaca ggtggtaatg ccatggttaa
    38041 ggcggcttat tcatgtggta aaccagctct tggggtaggt gccggaaacg ttccagctta
    38101 tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat atcgtcatgt ctaaatcatt
    38161 tgataacggt atggtctgtg catctgaaca agcagttatc attgataaag aaatttacga
    38221 tgaatttgta gcagagttca aatcttacca cacttacttt gtaaacaaaa aagaaaaagc
    38281 tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc aaaaactgtg ctggtgcaaa
    38341 attgaacgct gacatcgttg gtaaaccagc aacttggatt gcagaacaag caggatttac
    38401 agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa gaagttggcg aaaatgagcc
    38461 attgactcgt gaaaaattgt caccagttat tgcagttttg aaatctgaaa gccgtgaaga
    38521 tggtattact aaggctcgtc aaatggttga atttaacggt cttggacact cagcagctat
    38581 ccacacagct gacgaagaat tgactaaaga atttggtaaa gctgttaaag ctattcgtgt
    38641 tatctgtaac tcaccttcta cttttggtgg tatcggggac gtttacaatg ccttcttgcc
    38701 atcattgaca cttggatgtg gttcttacgg acgcaactca gttggggata acgttagtgc
    38761 cattaacctc ttgaatatca aaaaagtcgg aagacggaga aataacatgc aatggatgaa
    38821 acttccttca aaaacatact ttgaacgtga ttcaattcaa taccttcaaa aatgtcgtga
    38881 cgttgaacgt gtcatgatcg ttactgacca tgccatggta gagcttggtt tccttgatcg
    38941 tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt taccaaatct ttgcggatgt
    39001 agaaccggat ccagatatca caactgtaaa ccgtggtact gagattatgc gtgccttcaa
    39061 accagatacc atcatcgcac tcggtggtgg gtctccaatg gatgctgcca aagtaatgtg
    39121 gctcttctac gagcaaccag aagtggactt ccgtgacctt gtccaaaaat tcatggatat
    39181 ccgtaaacgt gccttcaagt tcccattgct tggtaagaag actaaattca tcgcgattcc
    39241 aactacatct ggtacaggat ctgaagtaac accatttgcc gttatctctg ataaagcaaa
    39301 caaccgtaaa tacccaatcg ctgactactc attgacacca actgtggcaa tcgtagatcc
    39361 tgctttggta ttgacagttc caggatttgt tgctgctgat actggtatgg acgtattgac
    39421 tcacgcgaca gaagcatacg tatcacaaat ggctagtgac tacactgatg gtttagcact
    39481 tcaagctatt aaattggtct ttgaaaatct cgaaagctca gttaaaaatg cagacttcca
    39541 ctcacgtgag aaaatgcata acgcttcaac aatcgctggt atggcctttg ccaatgcctt
    39601 cctaggtatt tctcactcaa tggcccataa gattggtgcg caattccaca cagttcacgg
    39661 acgtacaaat gccatcttgc ttccgtacgt tatccgttac aacggtacac gtccagctaa
    39721 gacagcaaca tggcctaagt acaactacta ccgcgcagat gaaaaatacc aagatatcgc
    39781 acgcatgctt ggacttccag cttctactcc agaagaaggg gttgaatctt acgcaaaagc
    39841 tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat tttagagacc aaggaattga
    39901 cgaaaaagaa tggaaagaac attctcgtga attagccttc ctggcttatg aagaccaatg
    39961 ttcaccagct aacccacgtc ttccaatggt agaccatatg caagaaatca tcgaagatgc
    40021 atactatggc tacaaagaaa gaccaggacg ccgtaaataa ttgtttatca gtctagaagc
    40081 aagacaaaaa ctcaatttga gggaaagatc cagtaatttt tctatgataa aaggcatcct
    40141 atcaaggttt ttgaacacct gataggatgc ctttttatga tattgaggcc tttttgccct
    40201 ttttgaaaaa ctagaataga aacaaaatat ataatagatt gaaactagag tagtacacat
    40261 ctgcttctaa aacattgtta gaa
//
LOCUS       DAVLSL010000003        34891 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLSL010000003 DAVLSL010000000
VERSION     DAVLSL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.003"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            161..457
                     /locus_tag="VXN30_000113"
     CDS             161..457
                     /locus_tag="VXN30_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1356796.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            534..2564
                     /locus_tag="VXN30_000114"
     CDS             534..2564
                     /locus_tag="VXN30_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1356797.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQGLFTDYL
                     QNFSSESVEVTSQKYYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDCLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            2566..3207
                     /locus_tag="VXN30_000115"
     CDS             2566..3207
                     /locus_tag="VXN30_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356798.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            3200..3493
                     /locus_tag="VXN30_000116"
     CDS             3200..3493
                     /locus_tag="VXN30_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356799.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            3744..4616
                     /gene="rsmA"
                     /locus_tag="VXN30_000117"
     CDS             3744..4616
                     /gene="rsmA"
                     /locus_tag="VXN30_000117"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1356800.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            4618..5496
                     /gene="rsgA"
                     /locus_tag="VXN30_000118"
     CDS             4618..5496
                     /gene="rsgA"
                     /locus_tag="VXN30_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1356801.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            5507..6163
                     /locus_tag="VXN30_000119"
     CDS             5507..6163
                     /locus_tag="VXN30_000119"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1356802.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            6126..6788
                     /locus_tag="VXN30_000120"
     CDS             6126..6788
                     /locus_tag="VXN30_000120"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1356803.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            6790..8046
                     /locus_tag="VXN30_000121"
     CDS             6790..8046
                     /locus_tag="VXN30_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1356804.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLYFQENEEEYED"
     gene            8036..8977
                     /locus_tag="VXN30_000122"
     CDS             8036..8977
                     /locus_tag="VXN30_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1356805.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            9244..10071
                     /gene="purR"
                     /locus_tag="VXN30_000123"
     CDS             9244..10071
                     /gene="purR"
                     /locus_tag="VXN30_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1356806.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            10138..11388
                     /locus_tag="VXN30_000124"
     CDS             10138..11388
                     /locus_tag="VXN30_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1356807.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            11514..12308
                     /gene="pflA"
                     /locus_tag="VXN30_000125"
     CDS             11514..12308
                     /gene="pflA"
                     /locus_tag="VXN30_000125"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1356808.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(12383..13309)
                     /gene="yidC"
                     /locus_tag="VXN30_000126"
     CDS             complement(12383..13309)
                     /gene="yidC"
                     /locus_tag="VXN30_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1356809.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(13387..13665)
                     /locus_tag="VXN30_000127"
     CDS             complement(13387..13665)
                     /locus_tag="VXN30_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1356810.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            13699..14439
                     /locus_tag="VXN30_000128"
     CDS             13699..14439
                     /locus_tag="VXN30_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1356811.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            14495..15178
                     /locus_tag="VXN30_000129"
     CDS             14495..15178
                     /locus_tag="VXN30_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1356812.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            15290..16282
                     /locus_tag="VXN30_000130"
     CDS             15290..16282
                     /locus_tag="VXN30_000130"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1356813.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            16347..16886
                     /gene="rsmD"
                     /locus_tag="VXN30_000131"
     CDS             16347..16886
                     /gene="rsmD"
                     /locus_tag="VXN30_000131"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1356814.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            16876..17364
                     /gene="coaD"
                     /locus_tag="VXN30_000132"
     CDS             16876..17364
                     /gene="coaD"
                     /locus_tag="VXN30_000132"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1356815.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            17348..18385
                     /locus_tag="VXN30_000133"
     CDS             17348..18385
                     /locus_tag="VXN30_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1356816.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            18443..18673
                     /locus_tag="VXN30_000134"
     CDS             18443..18673
                     /locus_tag="VXN30_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1356817.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            18697..19980
                     /gene="murA"
                     /locus_tag="VXN30_000135"
     CDS             18697..19980
                     /gene="murA"
                     /locus_tag="VXN30_000135"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1356818.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            19973..20161
                     /locus_tag="VXN30_000136"
     CDS             19973..20161
                     /locus_tag="VXN30_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1356819.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            20200..21024
                     /gene="endA"
                     /locus_tag="VXN30_000137"
     CDS             20200..21024
                     /gene="endA"
                     /locus_tag="VXN30_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1356820.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            21315..22646
                     /locus_tag="VXN30_000138"
     CDS             21315..22646
                     /locus_tag="VXN30_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1356821.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELISQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(22844..22914)
                     /locus_tag="VXN30_000139"
     tRNA            complement(22844..22914)
                     /locus_tag="VXN30_000139"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22880..22882),aa:Cys,seq:gca)
     gene            23330..26941
                     /gene="rpoB"
                     /locus_tag="VXN30_000140"
     CDS             23330..26941
                     /gene="rpoB"
                     /locus_tag="VXN30_000140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1356822.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            26974..30651
                     /gene="rpoC"
                     /locus_tag="VXN30_000141"
     CDS             26974..30651
                     /gene="rpoC"
                     /locus_tag="VXN30_000141"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1356823.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            30762..31175
                     /gene="ndk"
                     /locus_tag="VXN30_000142"
     CDS             30762..31175
                     /gene="ndk"
                     /locus_tag="VXN30_000142"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1356824.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(32104..32736)
                     /locus_tag="VXN30_000143"
     CDS             complement(32104..32736)
                     /locus_tag="VXN30_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356825.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(32738..34753)
                     /locus_tag="VXN30_000144"
     CDS             complement(32738..34753)
                     /locus_tag="VXN30_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1356826.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
BASE COUNT        10167 a         6848 c         7972 g         9904 t
ORIGIN      
        1 aatgactata aaagttcatt tcagggtgct attttgacaa tttagagaaa aacaaataat
       61 tttagaatat catttggtaa tataatagta tcaacgttga taatggaaag aggagcaagt
      121 aactcaacaa tcttgtaatc aaaaatagag gagtatcaat atgaaacaaa cagttaaaaa
      181 gttagctctt gtagcgagca ttgcagcaac attaggtgga ggtgtatcag ttgcctctgc
      241 agccgttcaa tacccagaag gtggtgtttg gacttatggt tcaggtaacg gtggtgctta
      301 ctcaaactac tatcaccctt caaaatacca tagttcaaca gttgttagca gaaaaactgg
      361 ttcatctgac aagggatatg ctggtgctgg agggacttct cgtgcatgga ttcgtacttc
      421 ttggggagag aaagttgcat tctactataa tgtttagaat ggtatagccc tattgtttag
      481 ctttacttgg tcaccttctc actaagtgac caagtaactt tttggaggaa atgatgaaac
      541 gtttatttat tttgatttca atggtattgg tatcgcttta tatggtgata acttccgttg
      601 accatcgaga agagatttta tttggtaact atccttctgt tgatgtgaca ggaatgatga
      661 taaatcaacc agtagctagt cgcgaagagg tgacagaggc tttgagtcac ttggcggtag
      721 agcataatag tctcatcgcc cgtcgaatcg ttgagccaaa tgaagctgga gaaacactct
      781 ttacctatgc cacttatggt gagggagagc ttccagaagg tctgaccatt tcctccaagg
      841 agagtgcaga aacgagtgat ttattagggt cttacttgat tgtgtcagga agtttggatg
      901 gagtgagctt acagaccacc ttgaaagaac ttggctatca aggctttgtt tcgaatggag
      961 aagatccatt ttcgatagtc ttactattga cggccacccc tatggtgcta ctgagtttag
     1021 ctatttttct gctgaccttt atgagtctga ccctgattta tcggatcaaa tcccttcgtc
     1081 aggcagggat tcgcttaata gctggtgaga gcttgtttgg agttgctctc agaccagtgt
     1141 tagaagatgt gagacagctt atctgctcag tgctggtatc cagtcttttg ggattgggga
     1201 ttctctggta tcaaggtgcc ttgtttatgg caacggtgca actggtcatc attgctcttc
     1261 tactttatgg attgaccctg gcagggattt ctaccttact aagtgtcgtc tatctacttg
     1321 gtttacagga aaatagtctg gtggatctat tgaaagggaa actccctctc aaacgtatga
     1381 tgacattgat gatggtgggg caactcttag ctgtattggt ggtcggatcg agtgcgacag
     1441 ctctcctacc ccactaccgt gaaatgcagg aaatggagag agctagcaat aaatggagcc
     1501 agtcctcaga ccgttaccgt ctatcctttg gttggtctag tgcatttgcc gatgaagaag
     1561 gaacgcgtaa ggataatcgt gagtggcaga catttactga agaacggtta gccaatacag
     1621 actcttttta tattatgagc aatgttgaca atttctcaga tggagcagaa gtggacctag
     1681 atggcaatcg tctcagtgac tacacaccgt cagggaatgt tatctatgtc tcaccacgct
     1741 atctgataga agaaaagatt accgtttctt cagagtttat ggacaagatg caaaacttgt
     1801 ctgagggaga gtttgggctg atcttgcctg agagcttgcg agagcagtct acctactacc
     1861 aaggattgtt tacagattac ctgcaaaact tttcatctga aagtgtagaa gtgacgagtc
     1921 agaaatacta cctcccacag gtaaggctag cttttacaga aacaggacag gaacgtttcc
     1981 tctataatga tgggtacaag acaacacgcc agtacctaaa agatccgatt attgtagttc
     2041 taacgccgca agcgactgga acaagacctg ttgcagggat gttgtgggga actacggcta
     2101 atagtgcctt gaaactagat cgatatggag acagcatcac agctctaaaa gagcaaggtc
     2161 tgtatcacaa ggtttcttac ttggtaaaaa gccagctatt ttttgccaag gtactaaatg
     2221 acaaacgggt ggagttttac tctctcctta ttgggacgat tttgaccctg tctacggcta
     2281 tcttgttatt tgattccatg aatcttctct attttgagca gttcagacgg gaacttatga
     2341 ttaaacgtct tgctggtatg acaatctatg agcttcatgg caagtattta ctggcgcaag
     2401 gaggagttct cttgcttggc ctagtcctat ctagtatttt gacaagagat tgtttgatta
     2461 gcgctctagt tgtagctttg tttacgctta acgccctctt gattttagta aggcaggaca
     2521 aaaaagaaga agctggtagc atggcagtat tgaaaggaaa ataagatgat tgatattcaa
     2581 ggattggaaa agaaatttaa tgaccgcgcg attttctctg gtttgaatct caagctggag
     2641 aagggcaagg tttatgcctt aatcggaaag agtggaagcg gaaagacgac gctgctgaat
     2701 atcttgggaa agctagaaaa gatagatggt ggaagggttc tctatcaggg gaaagattta
     2761 aaaaccattc ccactcgtga gtattttcga gaccagatgg gctatctctt tcaaaatttc
     2821 ggcctcttag aaaaccaatc aatcaaagaa aatttggatt tgggttttgt tggtcagaaa
     2881 atctcaaaag tagaacgttt ggaaaggcaa gtgggggctt tagaaaaagt taatctaggg
     2941 tatttggatt tagaacaaaa aatctatact ttatctgggg gagaggccca acgagttgcc
     3001 cttgctaaga ctattttgaa aaatccaccc ttgattttgg cagatgaacc aacagcagct
     3061 cttgatcctg aaaattcaga ggaggttatg aatctcttgg tggatttgaa agatgaaaat
     3121 cgaattatca tcattgcgac ccataatccc ctagtctgga ataaggctga tgaaatcatt
     3181 gatatgagga aacttgctca tgtgtgaaaa aatccgtatt cgcagggtat ctgattatcc
     3241 tagtgccaga ggtggtttag aagatatcct catcatggaa aatatgacca atcatctcct
     3301 tttggttcaa atccgagtgc atggctattt gcttgatttt gctagtattg aagggcaaag
     3361 gcaaaagcat tatcgtttga aaaatttacc tcagacggtt gaactgacag tggatgatgt
     3421 ggaggaggat gtggatttga ccctacccga aaatcgaagt tatcaagaag ctgatttttt
     3481 tgatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg tacttgagta
     3541 cggcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtatgaac gtatgtttcg
     3601 agagaagcac taagtccctc tttaaagatt tccaagacta tctttcttca tgaggaaaga
     3661 tagttttttg gtatgatttt cattcccaaa atacaagggg aatgtgttac aatagtagta
     3721 acagataata gaaaagagaa tagatgagaa ttgcagatta tagcgtgacc aaggcagtgt
     3781 tggagcgtca cggttttacc tttaaaaagt cctttgggca aaatttcttg acggatacca
     3841 atatccttca aaaaattgtg gatacggctg aaattgatga tcaggtcaat gtcatcgaaa
     3901 tcgggccagg tattggtgcc ttgacagaat ttttggctga gcgtgcagcc caagtcatgg
     3961 cttttgagat tgaccaccgt ttgatgccaa ttttggcaga tactctgcgt gattttgata
     4021 atgtgaccgt agttaacgaa gatattctca aggttgattt ggcgcaacat atccaaaatt
     4081 ttaaaaatcc taacctgcca atcaaggtag tggctaattt gccttactac atcacgacgc
     4141 ctattctcat gcacttgatt gagagtggca ttcctttttg tgagtttgtg gtcatgatgc
     4201 agaaagaagt agcggaccgc atttcagccc agcctaacac caaggcttac ggtagcttgt
     4261 ctatcgccgt gcagtattac atgacagcca aggttgcctt tatcgtgcct cgtacggtct
     4321 ttgtgccagc gccaaatgtg gattcagcca tcttgaaaat ggtgcgtcgt ccagagccag
     4381 ccgtagcagt agaagatgag aactttttct ttaaggtttc caaggctagt tttacccatc
     4441 gccgcaagac cttgtggaat aacttgacag gttactttgg taagactgaa gaggtcaagg
     4501 acaagctgac caaggctttg gaccaggcag gcttgtcacc aagtgtgcgt ggggaagctc
     4561 tcagcttggc agaatttgcc ggtctagcag acgcacttaa agggcaagga ctctaagatg
     4621 cagggacaaa tcattaaagc cttggcaggt ttctactatg tggagagtga tggccaggtt
     4681 tatcaaacac gcgcgcgtgg gaatttccgt aaaaaaggcc atacccctta tgttggggac
     4741 tgggtagatt tctctgccga ggaaaattca gaaggctata tcctcaaaat tcacgaacgg
     4801 aaaaacagtc tggttcgtcc gcctattgtc aatatcgatc aagctgtagt aatcatgtcc
     4861 gtcaaggaac ctgattttaa cagcaatttg ctggatcgtt tcttggttct tttggagcac
     4921 aagggcatcc atcccattgt ctatatttcc aaaatggatt tgttggaaga taggggagaa
     4981 ctggattttt accagcagac ctatggtgac atcggctatg actttgtgac cagtaaagag
     5041 gaactcctgt ctttgttaac aggcaaggtt acggtcttta tggggcagac aggtgttggg
     5101 aagtcaactc ttctcaataa aatcgcacca gacctcaatc ttgaaacggg agaaatttca
     5161 gacagtctag gtcgcggtcg ccataccact cgagctgtta gtttttacaa tctcaacggg
     5221 ggtaaaatcg cagatacacc aggcttttca tctctggact atgaagtatc aagggctgaa
     5281 gacctcaatc aggcctttcc agagattgct actgttagcc gagattgtaa gttccgtact
     5341 tgtacccata cccatgagcc gtcttgtgcc gtcaaaccag ctgttgaaga gggtgttatt
     5401 gcaaccttcc gttttgacaa ttatctgcaa ttccttagtg aaattgaaaa tcgtagagaa
     5461 acctataaaa aagtcagcaa aaaaattcca aaataaggag aaacctatgt ctcaatacaa
     5521 gattgctccg tcaattctgg cagcagatta tgccaacttt gaacgtgaaa tcaaacgtct
     5581 agaagcaact ggggcagaat atgcccatat cgatatcatg gacagtcatt ttgtaccgca
     5641 aatcagtttt ggtgcaggtg tggtcgagag ccttcgtcct catagtaaga tggttttcga
     5701 ttgccacttg atggtgtcaa accctgagca tcatctggaa gattttgcgc gtgcaggtgc
     5761 agacatcatc agtatccatg tagaagcaac gcctcatatt catggcgccc tccaaaaaat
     5821 tcgttcactc ggagttaagc cttcagtcgt tatcaatcct ggcacaccag ttgaagccat
     5881 caagcacgtc cttcatctag ttgaccaagt tttagtcatg acggttaatc caggttttgg
     5941 tgggcaagcc tttctgccag aaaccatgga taaggttcgt gagttggttg ctcttcgtga
     6001 ggaaaaaggt ttgaactttg aaatcgaagt ggatggtggg attgatgacc aaactattgc
     6061 tcaagccaaa gaagctggtg cgactgtttt tgtagcaggt tcctatgtct ttaagggaga
     6121 agtcaatgag cgagtacaaa ctctcagaaa acaactggac tagggttgca gtttttgcag
     6181 gcggaaaccg cggtcattat cggacagatt ttgatgcttt tgttggggtg gatcgaggct
     6241 cgctctgggt cttggaagaa gacttacctc ttgctctagc agtcggagat tttgattctg
     6301 tgacggaaga agagcgacag gtgattcaaa aacgtgccca gtattttgtc caagcccgac
     6361 cagaaaaaga tgataccgat ttggaattgg ctctcttaac catctttgag aaaaatcctc
     6421 aggctcaggt tactattttc ggtgccttgg gtggccgtat tgaccatatg ttggccaatg
     6481 tctttctgcc tagcaatcct aagttggcac cctatatgca tcaaatagaa attgaggatg
     6541 ggcaaaactt gattacttat tgtccagaag ggaccagtca gctagaacct cgttcagact
     6601 acgactatct agcctttatg ccagttcggg atagccagct gactattctt ggagccaagt
     6661 atgagttgac agaggaaaat tttttcttta aaaaagtgta cgcttctaac gaatatatag
     6721 atagggaagt gtcggtaact tgcccagatg gttatgtggt cgtactgcat agcaaggata
     6781 ggaggtagga tggaaagttt acttattcta ttattaattg ccaacctagc tggtctcttt
     6841 ctgatttggc aaaggcagga taggcaggag aaacacttaa gtaagagctt ggaggatcag
     6901 gcagatcatt tgtcagacca gttggattac cgctttgacc aagccagaca agccagccag
     6961 ttagaccaaa aagatttgga agtggctgtc agcgaccgtt tgcaagaagt gcggattgaa
     7021 ttgcaccaag gcctgaccca agtccgtcaa gaaatgacag ataatctcct ccaaactaga
     7081 gataagacag accaacgtct ccaagccttg caggaatcaa atgagcaacg tttggaacaa
     7141 atgcgccaga cggtcgagga aaaactagaa aagaccttgc agacacgctt acaggcttcc
     7201 tttgagacag tttctaaaca actggagtct gtcaatcgtg gtcttggaga aatgcagaca
     7261 gttgcccgtg atgtcggagc tcttaacaag gttctctctg gaaccaagac gcgagggatt
     7321 ctgggagaat tgcaactggg gcaaattatt gaagacatca tgacacctgc ccagtacgaa
     7381 cgagaatacg caacggttga aaactctagt gaacgagtgg agtatgccat caagttaccc
     7441 ggacaaggcg accaagaata cgtctatctt ccaattgact ctaagtttcc actggcagat
     7501 tattaccgct tggaagaagc ctatgagaca ggtgacaagg atgagattga acgctgtcgt
     7561 aagtcactcc tagcaagcgt caagcgcttt gccaaggata tcaagagcaa gtacatagca
     7621 ccgcctcgga caaccaactt tggaatcttg tttgttccga cagaaggtct ttattcagag
     7681 attgtgcgca atccggtctt ctttgatgat ttgagacggg aagaacagat tattgttgca
     7741 ggaccaagta ccctatcagc ccttcttaac tccctatcag ttggtttcaa gacccttaat
     7801 atccaaaaga gtgccgacca tatcagcaag actcttgcca gtgtcaagac cgagtttggc
     7861 aagtttggtg gtattctggt caaggcacaa aaacatctcc aacatgcctc tggcaatatt
     7921 gatgaattat taaaccgtcg taccatagct atcgagcgga cgctccgtca cattgagttg
     7981 tcagaaggtg agcctgcgct tgatctactc tattttcaag aaaatgagga agaatatgaa
     8041 gattagtcac atgaaaaaag atgagttatt tgaaggcttt tacctaatca aatcagctga
     8101 cctgaggcaa actcgagctg ggaaaaacta cctagccttt accttccaag atgatagtgg
     8161 cgagattgat gggaagctct gggatgccca acctcataac attgaggcct ttaccgcagg
     8221 taaggttgtc cacatgaaag gacgccgaga agtttataac aatacccctc aagtcaatca
     8281 aattactctc cgcctgcctc aagctggtga acccaatgac ccagctgatt tcaaggtcaa
     8341 gtcaccagtt gatgtcaagg aaattcgtga ctacatgtcg caaatgattt tcaaaattga
     8401 aaatcctgtc tggcaacgga ttgtccgaaa tctctacacc aagtatgata aggaattcta
     8461 ctcctatcca gccgccaaga ccaaccacca tgcctttgaa acgggcttgg cttatcatac
     8521 ggcgaccatg gtacgtttgg cagacgctat tagcgaagtt tatcctcagc tcaataagag
     8581 cttactctat gcggggatta tgctgcatga cttagctaag gttatcgagt tgacggggcc
     8641 agaccagaca gagtacacag tgcgaggtaa tcttcttgga catatcgctc tcattgatag
     8701 cgaaattacc aagacagtta tggaactcgg catcgatgat accaaggaag aagtcgtttt
     8761 gcttcgtcat gtcatcctca gtcaccacgg cttgcttgaa tatggaagtc cagtccgtcc
     8821 acgtattatg gaggcagaga ttatccatat gattgacaat ctggatgcaa gcatgatgat
     8881 gatgtcaaca gctctggctt tggtggataa aggagagatg accaataaaa tcttcgctat
     8941 ggataatcgt tccttctata aaccagattt agattgataa tttaagaaaa atgagcattt
     9001 tttaggataa gaatgttcgt ttttttatgt gaatatggta taataagtaa aagacaaaaa
     9061 tgaatactct tcgaaaatct cttcaaacta gggtagtatc gccttgtcgt atgtatatat
     9121 gcaggtatat tacagggttt gtcagttcta tcgacaatct caaagcagtg ttttgaacca
     9181 ccagcgacca gctttctagt ttgctttttg attttttgaa taaaaatgga ataggaaata
     9241 gaaatgaaat taagaagaag tgatcggatg gttgtcattt ccaactattt gattaataat
     9301 ccttataaac taactagtct caatactttt gctgaaaagt atgagtctgc taaatcatcc
     9361 atctcagaag atatcgtcat tatcaaacgc gcctttgagg aaattgaaat cggtcatatc
     9421 cagacagtga ctggggctgg cggaggtgtc atcttcacac cgtctatttc gagtcaggat
     9481 gctaaggaaa tggttgaaga cttgcgtacc aagttgtcag aaagtgaccg tatcttgcca
     9541 ggtggttata tctatctgtc tgatttgctt agcacaccag ccatcttgaa aaatattggt
     9601 cgtattattg ccaaaagctt tatggaccaa aaaattgacg cggttatgac cgtagcaact
     9661 aagggtgtgc cacttgcaaa tgcagttgcc aatgtcctca atgtctcttt tgtcattgtg
     9721 cgccgtgacc tgaaaattac cgaaggttca actgttagcg tcaactatgt ttcaggttca
     9781 agtggtgacc gtatcgagaa aatgttcctt tcaaaacgta gtcttaaggc aggtagccgt
     9841 gtcttgattg tggatgactt cttgaaaggt ggcggaacgg tcaacggtat gattagtctc
     9901 ttgcgcgagt tcgactcaga actggcaggt gtagcggtct ttgcggacaa tgcccaagaa
     9961 gaacgtgaaa agcagtttga ctacaagtca ctcttgaagg taactaatat tgatgtcaag
    10021 aaccaagcca tcgatgttga ggttggcaat atctttgacg aagataaata agagatagaa
    10081 ctaaaggttg gaacgattgt cccagccttt ctttgcaaac agaatagaag gaagcttatg
    10141 aaaacaccat ttatcaatag agaagagtta gaagcgattg ttgccgagtt cccgactccc
    10201 tttcacttgt atgatgagaa ggggattcgt gagaaggcaa gagccgtcaa ccaagctttt
    10261 tcgtggaaca agggctttaa ggaatatttt gcagttaagg ctactccaac tccagctatt
    10321 ttgaaaattc tccaagaaga aggttgtggt gtggactgct ctagttatgt agagcttttg
    10381 atgagccata aactggactt tctgggttct gagattatgt tctcttccaa caacacgcca
    10441 gacaaggaat acgcctatgc acgtgaattg ggtgcgacca ttaacttgga tgcctttgaa
    10501 gatattgaac atctggagag agtagcaggc attccagaaa tcatctcttg tcgttataat
    10561 cctggaggcg tttttgaact ggggacagac attatggaca atcctgggga ggctaagttt
    10621 ggcatgacca aggaccagct ctttgaagcc tttgctatct tgaaggaaaa aggagccaag
    10681 acttttggga ttcactcctt cctagcgtcc aataccgtga cccatctcta ttatccagag
    10741 ttggctcgtc agctctttga actggctgtt gaaatcaagg aaaagttggg catttggcta
    10801 gactttatca atctttctgg cggtattggt gttaattatc gtccagacca ggagccgaac
    10861 gatatcgcct tgattggtga gggagttcgt aaggtgtatg aagaagttct tacgtcagca
    10921 ggtcttggtc aggtcaagat tttcaccgaa ttgggtcgtt ttatgctggc acctcacggt
    10981 gctctagtca caagagtgac tcataagaag gaaacctacc gtacctatct aggtgtggat
    11041 gcctcagcag tcaacctcat gcgtccagct atgtacggag cttaccatca tattagcaac
    11101 gtgacccatc cagatggacc agctgaagcg gtagatgtgg ttggttcact ctgtgaaaac
    11161 aatgataaat ttgcagtcaa tcgcgaactg cctcatacag aaatcggtga tttgctggta
    11221 attcatgata caggtgccca cggattttca atgggctacc agtataatgc caaattacgt
    11281 tctgcggaaa tcctctatac cgaagaaggt aaagcccgtc aaatccgccg tgcagagcgc
    11341 cctgaggact attttgcaac cttatatggc ttcgattttg aagaataatc tgataataga
    11401 ttgaaaatga aattgaaaaa cagattgctt tctaaaaaat aggcaaaaat cttgtttttc
    11461 cttcaagtcg tgatataata aaactataaa acgttttcaa ggaaggtaac gatatgtctg
    11521 aagaaacaat tgattatgga caagtgacag gaatggtgca ttcgacagaa agctttgggt
    11581 cagtagatgg gcctggtatt cgctttattg tctttttgca gggctgtcac atgcgttgcc
    11641 agtattgcca caacccagac acttgggcta tggagtccaa taagtcacgt gaacggacgg
    11701 tagatgatgt cttgacagag gccttgcgct accgtggttt ctggggaaat aagggtggga
    11761 ttacagtcag tggaggagaa gctctcttgc agattgattt cctgattgct ctcttcacca
    11821 aggctaagga acaaggaatc cactgtacct tggatacctg tgctcttcct ttccgtaata
    11881 aaccacgtta ccttgagaag tttgacaaac tcatggctgt cactgacttg gttcttttgg
    11941 atatcaagga aatcaacgaa gaacagcaca agattgtcac tagacaaacc aataaaaata
    12001 tcttggcttg tgcccagtat ctatcagata ttggaaaacc tgtctggatt cgccacgtgc
    12061 tagttccagg attgacagac agagatgatg acttgattga acttggtaag ttcgtcaaga
    12121 ccctcaaaaa tgttgataag tttgaaattc taccttatca caccatgggt gagttcaagt
    12181 ggcgtgaact tggaattcca tattccctcg aaggagtcaa accaccaaca gcagatcgcg
    12241 tcaagaacgc taaaaaactc atggataccg aaagttatca agattatatg aaacgtgtac
    12301 atggatagaa aagaagcctg atggaaacat cgggcttttg atttgcaaaa agacttagca
    12361 aatcagctaa gcctttttct tcttatctcg aacgttgttt tccagcgttg cgatttttgt
    12421 gttttttctt gcttgtgata gcagttggtt gttcaggggt aacgtctttt cgtccacttg
    12481 gtttagagaa agcacttgct tttggtgggt tcttggctag ttcttcacgg acttttttgc
    12541 gaagttttgg acgaacgata tagttgacga taaactgttg gagaatcatc atgaaaccac
    12601 cgacaaccca gtaaagtgtg acactagctg gtgagaagag ggagaagacg acgatcatga
    12661 gtgggctcat gtaaatcatt ttcttgattt gttctctttg catttcatct tctactccgt
    12721 gaagtgaaag gagcgattga agatagtaaa ggacaccagc acaggcaacc aaaatcatac
    12781 ttggagaacc tagaggaatg cctaggtagc ttgcttgagc aaccccttca gtatgttggg
    12841 cagcaaagta gatagcagag aagaaaggca tttgaaggag gatagggaaa catcctacac
    12901 cgccaaacat gctgataccg tgctcttttt gagcagcaaa gagagcttgt tgggcttcga
    12961 gtttttcttc ttgagtagtc gcttctttga gacgcgtttg gtgtggctca aggacgtgct
    13021 tgagggcgtt catcttttca gagtgaagcg ttgccttcca tgattggtag ataccaagtg
    13081 gcaagataat caagcgcacg ataatggtta cgatgatgat agcgacacca aagcctagac
    13141 ctttatcagt agcgaagtac ttgatagctt cagccatagg ggctccgatg gtattccaaa
    13201 taaatcctgt tggctgacct gtggttttat cgacattgac acaaccagtc aagacaagca
    13261 acatagccgc tcccatagct gagagtgcaa aacgtttaat agatttcact gtttttattc
    13321 cttctttaaa aattatacct ttctattcta ctgttttttt acaaaatata caatagttct
    13381 ggagacctaa tttgcgattt taaagtcaga gtagggagga aagttgctta gtttgacatc
    13441 taagtagctt acttttgaaa aaggtgtcgg tccttttcgg atttcttgga taaattttgc
    13501 catgatagca gatgagtctg cttgggctaa gatttccact gtgccatcgt cgttattcca
    13561 tactcgacct gtgatgccac caatttcaag tgccaagctg taaacacccc aacgaaagcc
    13621 gactccctgt accctacctt gggcaatcat tctaaccttt tgcataccaa acccctttga
    13681 ttgtgttata atatttctat gactattata acctcaaaag ccaattctgt ggtaaaaaat
    13741 gccaagaaat tacgccaaaa aaaataccgc aagtctgcct acttgattga aggctggcac
    13801 ttgtttgaag aagctgtcca agctggagtg acgattgaga aaatctttgc cctagaaaat
    13861 tatcgagatc agttagctac tttttcgcaa actgtctggg tgtcagagga tattttgcta
    13921 gatttggcgg attctcagac tccacaggga attgttgccg tggttcaaaa agaagaagta
    13981 ggacaagctg atttgagtca gggcaagttc ttgtttttgg aagatgtgca agatcctggt
    14041 aatgtaggaa ctatcattcg aactgcggat gcagcaggtt ttacaggagt gattgtttca
    14101 gataagtcgg cagacatcta cagtctcaaa accctacgtt ccatgcaagg tagtcatttc
    14161 catctgccca tttaccggat gactagtcaa gcgcttcttg acgaaactaa aaaggtagct
    14221 atcccagtgc tagcaacaac cctatctaaa gattctgttg attacagaga actgccttct
    14281 atagaaaatt ttgtactagt tatgggaaat gagggtcaag gaattagtcc ccttatggct
    14341 gaaagtgcag accagttggt ccatattagc atgaaggggc aggccgagag tttgaatgtt
    14401 gcggttgcag ccggtatttt aatcttccat ttaagctaat tttaactttt tttgttataa
    14461 tcaaggaaag atgttcacag aaaaggagaa atggatgaat cacactatta tacatgaccg
    14521 tgcaggtctc aatcaatttt acgctaaggt ttatgccttt gttggtctgg gaatcggact
    14581 atctgctttg gtatcaggcc ttatgttgac ggtctttcag tctcagttgg tttacttttt
    14641 gatgcagggg cgtctctggt tgaccattgc tacttttgcg gagttggctt tggttttcgt
    14701 tgccagtagc atggcttcaa gaaatagtcc agctgctctt ccagtatttt tactttactc
    14761 cgttttaaac ggctttaccc ttagttttgt ggtggctttc tatactccgg gtacagtttt
    14821 atctgccttt gtatcgagcg cccttctctt ctttgtcatg gcggcagttg gtatttttac
    14881 caagaaagac ttgagtggga ttggtcgagc aatgatggca gctctcattg gtttactgat
    14941 tgctatggta gtcaatattt tcttggctag tggcttcttt gattatatga ttagcgtagc
    15001 catggttttg gttttttcag ggttgattgc ttgggataac caaaagattc gcctcgccta
    15061 tgaacaatca caaggtcgtg tagcgacagg ctgggtggtg tcaatggctc tcagcatcta
    15121 tcttgatttt atcaatcttt tcctaagtat tctacgtatc tttggtcgca atgactaaaa
    15181 ggtctatgaa atcagtgttt tgagtgacac tgattttttt taaattttct cttttctttt
    15241 cttggaaata ggtgtataat acgtttatta aatttttgag gagttgtcta tgaagaaaag
    15301 ttttatccat caacaagaag aaatttcctt tgtcaaaaac acttttaccc agtatttgaa
    15361 agataagcta gaagttgtcg aagttcaagg tcctatcttg agtaaggtcg gtgacggaat
    15421 gcaggacaac ctgtctggtg tggaaaatcc agtatcggtc aaggttctcc aaatccctga
    15481 tgctacttat gaagtggtgc actcacttgc taaatggaaa cgccacacct tggctcgttt
    15541 tggctttggt gaaggagagg gtctctttgt ccacatgaaa gcccttcgtc cagatgagga
    15601 ttccttggat gcaacccact ctgtttatgt tgaccagtgg gactgggaga aggttatccc
    15661 aaatggtaag cgtaacatcg tttatctaaa agaaacagtt gagaagattt ataaggctat
    15721 tcgcctgact gagctagctg ttgaagcccg ctatgacatc gagtctatct tgccaaaaca
    15781 aatcaccttt atccatacag aagaattggt agaacgctac ccagacttga cacctaaaga
    15841 acgtgaaaat gcgatttgta aagaatttgg agccgtcttt ttgattggta tcggtggcga
    15901 gttgccagat ggtaaaccgc acgatggacg tgcaccagac tatgatgact ggacaagcga
    15961 gtctgagaat ggctacaagg gtctaaatgg tgatattctt gtctggaatg agtctttagg
    16021 tggagccttt gagttgtctt ctatgggaat tcgtgtagat gaagaaactc ttagacgtca
    16081 ggtggaaatt acaggtgatg aagaccgctt ggaattggaa tggcacaagt ctttgttgaa
    16141 tggcctattc ccattgacaa tcggtggagg aattggacaa tctcgtatgg ccatgttcct
    16201 acttcgcaag agacacatcg gagaagtgca aacaagtgtt tggcctcaag aagtccgcga
    16261 tacttacgaa aatattttgt agagaatcga accgcaaggt tcggttttct ttctcttttt
    16321 gtctataatt tggtataata aacagtatga aaatcgtatc aggaatctat gggggacgtc
    16381 ccctcaagac actagaaggc aagacaacaa gacctacttc ggataaggtt aggggagcca
    16441 tttttaacat gattggtccc tactttgaag ggggacgagt cttggacctt tatgcaggta
    16501 gtggtggttt atctatcgaa gcagtatcgc gtggcatgtc cagtgctgtt ttggtggagc
    16561 gagaccgtaa ggctcagacc atcgtggctg aaaatatcca gatgaccaag gaagttggaa
    16621 aatttcaact cctcaagatg gatgcagaga gggcattgga acaggtatct ggggaatttg
    16681 acctcgtttt cttagaccct ccctatgcca aggaacaaat cgtagcagat attgaaaaaa
    16741 tggctaagag agagcttttt tctgaagatg ttatggttgt gtgcgagacg gataaggccg
    16801 ttgaacttcc agaagagatt gcctgtctgg gtatctggaa ggaaaagatt tatggaatta
    16861 gtaaggtgac agtctatgtc agataagatt ggcttattca caggctcatt tgatccgatg
    16921 acaaatgggc atctggatat cattgaacgg gcgagcagac tttttgataa gctttatgtg
    16981 ggtatttttt ttaatcccca caaacaagga tttctccctc ttgaaaatcg taaacggggg
    17041 ctagaaaagg ctttgggaca tttggaaaat gttgaagtcg tggcttctca tgatgaattg
    17101 gtggtcgatg ttgcaaaaag actgggggct actttcctag tacgggggtt gaggaatgcg
    17161 gcggatttgc aatatgaagc tagttttgat tactacaatc atcagctgtc ttctgatata
    17221 gagactattt atttacatag ccggcctgaa catctctata tcagttcatc aggtgttcga
    17281 gagcttttga agtttggtca ggatattgcc tgccatgttc ccgagagtat tttggaggaa
    17341 ataagaaatg aaaaaaaaga ttagatggcc cttatacgtc attgcggcct tgattgtgac
    17401 tttcttggca tttgtagtgc ccttgcctta ttatatagag gttccaggtg gttcggaaga
    17461 tattcgccaa gtccttaaag taaatgacac agaagataag gaagctggtg cctatcaatt
    17521 cgttacggtt ggtgttcaac atgccacttt agctcatatg atttatgctt ggttgacacc
    17581 ttttacagat attcgtagtg ctcaggagac tacaggtggt tcttccgatg ttgaatttat
    17641 gcgaatcaat caattctaca tgcaaacatc gcaaaatatg gccaagtatc aaggactaaa
    17701 aacagctggt aaggatatcg aactcaagta ctttggagtt tatgttttga atgtgacgga
    17761 taattcaacc tttaaaggga ttctcaatat ctctgataca gtcacagcag tcaatgatca
    17821 gacctttgat agttccaaag acttgattga ttacgtcagt tctcaaaaat taggggattc
    17881 cgtcaaggtc acctatgaag aggatgggca aaccaagtct gcagaaggaa agattatcac
    17941 cttggaaaat ggcaaaaatg gaattggaat cggcttgatt gaccgtacag aggtaatcag
    18001 caatgtccca attagctttt caacagctgg tattggcggc ccaagtgctg gtctcatgtt
    18061 tagtctagct atctatactc aaatagctca cccagatctt cgtaatggtc gtattgttgc
    18121 cggtacaggt accattgacc gcgatggtaa tgtgggagac attggaggta ttgataagaa
    18181 ggttgtagct tcggctaggg caggtgctgc tattttcttt gctcctgata accctgttag
    18241 cgaagaagaa caaaaggcgc atctggacgc gaaaaacaac taccaaacag ccctagaagc
    18301 ggctaaaaca atcaagacgg atatgaaaat cgtgcccgtt aaaaccctac aagatgcgat
    18361 tgattacttg aaaaacaatc cctaattttt gagtgaagtt tccaaactag cgcacaaaca
    18421 gagaagatgg tataatagtc aaatggttca attattattc actctaagta gtcatatgct
    18481 ctttatttat gtgagttttt accttttaaa ggatcttgtt agatgggaga aggttttaaa
    18541 agtgacagat gataatacaa gaaaagttcg tttattagta gcctttttta gcattgtcat
    18601 aggctacatc ctgagttctt tctttattag cctgtatcat ttgtggcaag aagcgcttag
    18661 aggattatta taaaatcaag agtaaaggaa acgagtatgg ataaaattgt ggttcaaggt
    18721 ggcgataatc gtctggtagg aagcgtgacg atcgagggag caaaaaatgc agtcttaccc
    18781 ttgttggcag cgactattct agcaagtgaa ggaaagaccg tcttgcagaa tgttccgatt
    18841 ttgtcggatg tctttattat gaatcaggta gttggtggtt tgaatgccaa ggttgacttt
    18901 gatgaggaag ctcatcttgt caaggtggat gctactggcg acatcactga ggaagcccct
    18961 tacaagtatg tcagcaagat gcgcgcctcc atcgttgtat tagggccaat ccttgcccgt
    19021 gtgggtcatg ccaaggtatc catgccaggt ggttgtacga ttggtagccg tcctattgat
    19081 cttcatttga aaggtctgga agctatgggg gttaagatta gtcagacagc tggttacatc
    19141 gaagccaagg cagaacgctt gcatggcgct catatctata tggactttcc aagtgttggt
    19201 gcaacgcaga acttgatgat ggcagcgact ctggctgatg gggtgacagt gattgagaat
    19261 gctgcgcgtg agcctgagat tgttgactta gccattctcc ttaatgaaat gggagccaag
    19321 gtaaaaggtg ctggtacaga gactataacc attactggtg ttgagaaact tcatggtacg
    19381 actcacaatg tagtccaaga ccgtatcgaa gcaggaacct ttatggtagc tgctgccatg
    19441 actggtggtg atgtcttgat tcgagacgct gtctgggagc acaaccgtcc cttgattgcc
    19501 aagttacttg aaatgggtgt tgaagtaatt gaagaagacg aaggaattcg tgttcgttct
    19561 caactagaaa atctaaaagc tgttcatgtg aaaaccttgc cccacccagg atttccaaca
    19621 gatatgcagg ctcaatttac agccttgatg acagttgcaa aaggcgaatc aaccatggtg
    19681 gagacagttt tcgaaaatcg tttccaacac ctagaagaga tgcgccgcat gggcttgcat
    19741 tctgagatta tccgtgatac agctcgtatt gttggtggac agcctttgca gggagcagaa
    19801 gttctttcaa ctgaccttcg tgccagtgca gccttgattt tgacaggttt ggtagcacag
    19861 ggagaaactg tggtcggtaa attggttcac ttggatagag gttactacgg tttccatgag
    19921 aagttggcgc agctaggtgc taagattcag cggattgagg caagcgatga agatgaataa
    19981 gaaatcaagc tacgtaatca atcgtttact tttagtcatc atagtactga ttttaggtac
    20041 tctggctcta ggaatcggtc taatggtagg ttatggaatc ttgggcaagg gtcaagatcc
    20101 atgggctatc ctgtctccag caaaatggca ggaattgatt cataaattta caggaaatta
    20161 ggctggagaa ccagcctttt tctaaagata aggagaaata tgaacaaaaa aacaagacag
    20221 acactaatcg gactgctagt gttattgctt ttgtctacag ggagctatta tatcaagcag
    20281 atgcagtcgg cacctaatag tcccaaaacc aatcttagtc agaaaaaaca agcgtctgaa
    20341 gctcctagtc aagcattggc agagagtgtc ttaacagacg cagtcaagag tcaaataaag
    20401 gggagtctgg agtggaatgg ctcaggtgct tttatcgtca atggtaataa aacaaatcta
    20461 gatgccaagg tttcaagtaa gccctacgct gacaataaaa caaagacagt gggcaaggaa
    20521 actgttccaa ccgtagctaa tgccctcttg tctaaggcca ctcgtcagta caagaatcgt
    20581 aaagaaactg ggaatggttc aacttcttgg actcctccag gttggcatca ggtcaagaat
    20641 ctaaagggct cttataccca tgcagtcgat agaggtcatt tgttaggcta tgccttaatc
    20701 ggtggtttgg atggttttga tgcctcaaca agcaatccta aaaacattgc tgttcagaca
    20761 gcctgggcaa atcaggcaca agccgagtat tcgactggtc aaaactacta tgaaagcaag
    20821 gtgcgtaaag ccttggacca aaacaagcgt gtccgttacc gtgtaaccct ttactacgct
    20881 tcaaacgagg atttagttcc ctcagcttca cagattgaag ccaagtcttc ggatggagaa
    20941 ttggaattca atgttctagt tcccaatgtt caaaagggac ttcaactgga ttaccgaact
    21001 ggagaagtaa ctgtaactca gtaaaagata agcctaaact cctatgtcac ttatggatgt
    21061 aggagttctt tttactagtt taagcagggc tagaacaggt actaagaaaa aatagtaact
    21121 tctaaaacta acttccagtt tcccacaacc tagcttttag tatgagaaaa acgcgtgaaa
    21181 tcagtggaaa tccgtcttag actaacttcc agttttggga gagagatgga agttacgcta
    21241 agaaaaaaat aatatgtatt ggattaaatt ttgtgatata ataaaggata agatactatt
    21301 ttaggagaag aactatggaa gacccaagta gtcagaattt gttgctacaa tttgttttat
    21361 tgtttatctt gacggtatta aatgcctttt tctcagccac agaaatggcc atggtttctc
    21421 ttaatcgtgc ccgagttgaa caaaaggcgg aagaaggaga cagacgctac atccgcctgc
    21481 tgaaggtact agaaaatcct aaccactttt tatcaaccat tcaggtagga atcaccctga
    21541 ttacaatctt atcaggggcg agtttaactg atactctagg acgtgtgatt gcctcttggc
    21601 ttgggaatgg cgaaacagca caagccgtgg caacttttct atccttggca tttttgacct
    21661 atatttccat cgtttttggg gaattatatc ctaagagaat cgctcttaat ctaaaggatg
    21721 ccttggcaat tcgtacagcg ccggttatca ttgggatcgg aaaactagtc agtccttttg
    21781 tttggctttt agctgcttcg accaatttct tgagccattt gactcctatg tcattcgatg
    21841 atgcggatga aaagatgacc cgtgatgaaa ttgcctatat gctgacaaat agtgaagaaa
    21901 cattggatgc tgatgagatt gagatgctac aaggtgtctt ttcgctcgat gaactgatgg
    21961 cacgagaggt tatggttcct cgaacggatg cctttatggt ggatattcag gatgatagtc
    22021 aagccattat ccaaagtatt ttaaaacaaa attattctcg tatcccggtt tatgatgggg
    22081 ataaggacaa tgtaattgga atcattcaca ccaagagtct ccttaaggca ggctttgtgg
    22141 acggttttga caatattgtt tggaagagaa ttttacaaga tccacttttt gtacctgaaa
    22201 ctatttttgt ggatgacttg ctaaaagaac tgcgaaatac ccaaagacaa atggcaatct
    22261 tgctggatga atacggtggt atggctggtt tggtgacttt ggaagacctc ttagaggaaa
    22321 ttgttggtga aattgatgat gaaacggaca aagcagcaat agatgttcat cagattggcg
    22381 aggataccta cattgttcaa ggaactatga ccctcaatga tttcaataat tactttaatg
    22441 ttgaactgga aagtgatgac gttgatacta tcgctggtta ttatttgaca ggagtgggaa
    22501 ccattccaac gactgagaaa ctcagctatg aattgatcag ccaaaacaaa cagtttatct
    22561 taaccaatga taaagtgaag aatggacgtg ttaccaaggt aaaaatccaa atcacagaag
    22621 tcgaaataga agaagaaaca gaataaacta gaggcttttg tcaccaggcg tgacgaagct
    22681 tttttcaatt gatttcaaaa gggaattatg ccatggaaag aactctgcca caaactcgct
    22741 ccaaaagttt ttaaagttat acgtatttat tcgagggaaa ataagaaaag cccttcaaat
    22801 tgggctttct gttcatttga aacctgataa atcaagttat agaaggcggt agacggattt
    22861 gaaccgacga tcaagctttt gcagagccgt gccttaccac ttggctatac cgccttaacg
    22921 tttattatta taccttgaaa atgattttca gtcaatagtt ttttcagttt tttgtaccat
    22981 agcgtgattc gaatgtgatg ccaagttttc tatgggaaaa aatttaccaa aaacctactg
    23041 aaaccccttg aaaaatctag ctaaataggg tataatatgg gtaatcattt gtcgtaggtt
    23101 ttgtctgaaa tattgtccag acaaggctca cagcagttaa atcttctgaa aaagtcagat
    23161 ttagctgctc tttttgtgct ttttttcaag attttgagca tttgtaacag aggcttaaag
    23221 attctgaaaa ttcgtcaaga ggacacggtg ataaggggtt tacaaccata tggcgattag
    23281 aaaagcctga ttgacaaggc ttggaactta tttacaaagg agaatcatct tggcaggaca
    23341 tgacgttcaa tacgggaaac atcgtacccg tcgtagtttt tcaagaatca aagaagttct
    23401 tgacttacca aatttgattg aaattcaaac tgactcattc aaagctttcc tagaccacgg
    23461 tcttaaggaa gtgtttgaag atgtattgcc aatttcaaac ttcacagaca caatggagtt
    23521 ggaatttgtt ggatatgaaa tcaaggaacc aaaatacacg ctagaagaag ctcgtatcca
    23581 cgatgctagc tactcagcac caatttttgt aaccttccgt ttgatcaata aagaaacagg
    23641 cgaaatcaag acccaagaag ttttctttgg tgatttccca atcatgacag aaatgggtac
    23701 tttcatcatc aatggtggtg aacgtattat cgtttctcag ttggtccgct caccaggtgt
    23761 ttactttaac gacaaagtag acaaaaatgg taaggtgggc tatggttcaa ctgttatccc
    23821 taaccgtgga gcttggttgg aacttgaaag cgactcaaaa gatatcacct acactcgtat
    23881 cgaccgtact cgtaagattc catttacaac cttggttcgt gctcttggtt tctcaggtga
    23941 tgatgaaatc tttgatatct ttggtgacag cgaattggtt cgcaacactg ttgaaaaaga
    24001 tatccacaag aatccaatgg actctcgtac agacgaagcc ttgaaagaaa tttacgaacg
    24061 ccttcgtcca ggtgagccta agacggctga aagctcacgt agcttgcttg tagctcgctt
    24121 ctttgaccca cgtcgttatg acttggcagc agttggtcgt tacaaaatca ataaaaaact
    24181 caatgttaaa acacgtttgc tcaaccaaac cattgcagag ccattggtag accctgaaac
    24241 tggagaaatc ttggtagaag ctggtacgat tatgactcgt agcgtgattg aaagcattga
    24301 aagccatttg gatggcgact tgaacaagat tgtctacatc ccaaacgatg cagccgttgt
    24361 gactgagcct gttgttcttc aaaaattcaa ggttattgct ccaactgatc cagatcgcgt
    24421 cgtaacgatc attggtaatg ctaacccaga tgacaaggtt cgtacggtga ctcctgcaga
    24481 tatccttgct gagatgagct acttcctcaa cttggctgaa ggacttggcc gtgtagatga
    24541 tatcgaccac cttggaaatc gtcgtatccg tgcggttggt gaattgcttg ccaaccaagt
    24601 acgtttggga ctttctcgta tggaacgtaa tgtccgtgaa cgtatgtctg ttcaggacaa
    24661 tgaagtcttg acaccacaac aaattatcaa tattcgtcct gtaacagctg cagttaaaga
    24721 attctttggt tcatcacagt tgtcacagtt catggaccaa cacaacccgc tttctgagtt
    24781 gtctcacaaa cgccgtttgt cagccttagg acctggtggt ttgactcgtg accgtgccgg
    24841 atatgaagtg cgtgacgtgc actacactca ctatggtcgt atgtgtccaa tcgagacacc
    24901 tgaaggacct aacatcggtt tgatcaataa cttgtcatct tacggacact tgaacaaata
    24961 tggttttgtt caaacaccat accgtaaggt tgaccgtgaa acaggtgttg tcacgaacga
    25021 aattgtttgg ttgacagctg atgaagaaga tgaatatact gtagctcagg ctaactctcg
    25081 tctgaatgaa gatggaacct ttgctgagaa gattgtcatg ggacgtcacc aaggggtcaa
    25141 ccaagagtat ccagctaata ttgttgacta catggacgtt tcaccaaaac aggtagttgc
    25201 cgttgcgaca gcatgtattc ctttcttgga aaacgatgac tccaaccgtg ccctcatggg
    25261 agccaatatg caacgtcagg ctgtgccatt gattaatcct caggcacctt acgttggtac
    25321 tggtatggaa taccaagcag cccacgattc tggtgcggct gtgattgctc agtatgatgg
    25381 taaagttact tacgcagatg ctgacaaggt agaagttcgt cgtgaagatg gttcattgga
    25441 tgtttaccac atccaaaaat tccgtcgttc aaactcaggt actgcttaca accaacgcac
    25501 tctcgtaaaa gttggtgatg tcgttgaaaa aggcgatttc atcgctgacg gaccttctat
    25561 ggaaaatgga gaaatggcgc ttggacaaaa cccaatcgtt gcctacatga cttgggaagg
    25621 ctacaacttc gaggatgccg ttatcatgag cgaacgcttg gtgaaggacg atgtctacac
    25681 atctgttcac cttgaagaat acgaatcaga aacgcgcgat acaaagcttg ggcctgaaga
    25741 aatcactcgc gaaattccaa acgttggtga agatgccctc aaagaccttg acgaaatggg
    25801 gattatccgt attggtgctg aggttaaaga aggtgatatt cttgtaggga aagtaacacc
    25861 taagggtgag aaagatcttt cagctgaaga acgtctcttg cacgctatct ttggagacaa
    25921 gtctcgtgaa gtgcgtgata cttctcttcg tgtaccacac ggtgccgatg gtgtcgttcg
    25981 tgatgttaag atctttacac gtgtaaatgg agatgagttg caatcaggtg ttaacatgtt
    26041 ggttcgtgtt tacatcgctc aaaaacgtaa gattaaggtc ggagataaaa tggccggacg
    26101 tcacggaaac aaaggggttg tctctcgtat cgttcctgta gaagacatgc cttaccttcc
    26161 agacggaact ccagtcgaca tcatgttgaa cccacttggg gtgccatcac gtatgaatat
    26221 cggtcaggtt atggagcttc accttggtat ggcagctcgt actcttggta ttcacattgc
    26281 gacaccagtc tttgatggag caagttctga agatctttgg tcaactgtta aagaagcagg
    26341 tatggatagc gatgccaaaa cgatccttta cgatggacgt actggtgaac catttgataa
    26401 ccgtgtttct gttggagtca tgtacatgat caaactccac cacatggttg acgataaatt
    26461 gcacgcgcgt tcagtcggac cttactcaac tgttacccaa caaccactcg gaggtaaagc
    26521 tcagtttggt ggacaacgtt tcggtgagat ggaggtttgg gctcttgaag cctacggtgc
    26581 gtcaaatgtc cttcaagaaa tcttgactta caagtctgac gatatcaacg gacgtttgaa
    26641 agcctatgaa gctattacaa aaggcaaacc aattccaaaa ccaggtgttc cagaatcctt
    26701 ccgagttctt gttaaagaat tgcaatctct tggtcttgac atgcgtgtcc tagacgaaga
    26761 tgaccaagaa gtggaacttc gcgacttgga tgaaggaatg gacgaagatg tcatccacgt
    26821 agatgacctt gaaaaagccc gcgaaaaagc agcccaagag gctaaagcag cctttgaagc
    26881 tgaagaagct gagaaagcaa caaaagcgga agcaacagaa gaagctgctg aacaagaata
    26941 agcagttcac ttagaataga aagggaagaa atagtggttg atgtaaatcg ttttaaaagt
    27001 atgcaaatca ccctagcttc tccaagtaaa gtccgttcat ggtcttatgg agaagtcaaa
    27061 aaacctgaaa caatcaatta tcgtaccttg aaaccagaac gtgaaggact ctttgatgaa
    27121 gtgatctttg gtcctacaaa agactgggaa tgtgcttgtg gtaagtacaa acgcattcgt
    27181 tacagaggaa ttgtttgtga ccgctgtggg gttgaagtaa cgcgtacgaa agttcgtcgt
    27241 gagcgtatgg gacatatcga attgaaagct cctgtatctc acatctggta cttcaagggg
    27301 attccaagcc gtatgggctt gacccttgat atgagccctc gtgccctcga ggaagttatc
    27361 tactttgcgg cttatgtggt gattgatcct aaggatacac cacttgagca caagtctatc
    27421 atgacagagc gcgaataccg agagcgcttg cgcgaatatg gttatggttc atttgttgcc
    27481 aagatgggtg cggaagccat ccaagacctt ttgaagcaag tagatcttga aaaagaaatt
    27541 gctgaactca aagaagaatt gaaaacagct actggacaaa aacgtgtcaa agccatccgt
    27601 cgtttggatg ttttggatgc cttttacaag tctggaaaca aacctgaatg gatgattctt
    27661 aacatccttc cggttatccc accagatctt cgtccaatgt tgcagttgga tggtggccgt
    27721 tttgcctcat ctgacttgaa tgacctttac cgccgtgtta tcaatcgtaa caaccgtttg
    27781 gctcgtttgc ttgagttaaa tgcaccaggt atcatcgttc aaaatgagaa acgtatgctt
    27841 caagaagcag ttgacgcttt gattgacaat ggtcgtcgtg gtcgtccaat cacaggacca
    27901 ggtagccgtc cattgaaatc attgagccac atgcttaaag gtaaacaagg acgtttccgt
    27961 caaaacttgc tcggtaaacg tgttgacttc tcaggacgtt cagttatcgc cgttggtcca
    28021 actcttaaga tgtaccaatg tggtgtgcca cgtgaaatgg cgattgaact ctttaaacca
    28081 tttgtcatgc gtgaaatcgt tgcccgtgat atcgtgcaaa acgtcaaagc agctaaacgc
    28141 ttggtggaac gcggagatga gcgtatctgg gatatccttg aagaagtgat taaagaacac
    28201 ccagtgcttt tgaaccgcgc accgaccctt caccgtttgg gtatccaagc cttcgagcca
    28261 gtcttgattg atggtaaggc tcttcgcttg cacccacttg tctgtgaagc ctacaatgct
    28321 gactttgacg gggaccaaat ggccatccac gtaccacttt cagaagaagc acaagcagaa
    28381 gctcgtatcc tcatgctagc tgctgagcac atcttgaacc cgaaagatgg gaaaccggta
    28441 gttactccat ctcaggacat ggttttgggt aactactact tgaccatgga agaagctggt
    28501 cgcgaaggtg aaggaatggt cttcaaagac cgtgacgaag cggttatggc ttaccgcaat
    28561 ggttatgttc acctccactc acgtgttggt atcgcaacag acagcctcaa caagccttgg
    28621 acagaagagc aaagacataa ggtcttgctt acaacagttg gtaaaattct cttcaacgat
    28681 atcatgccag aggggctacc atacttgcaa gaaccaaaca atgccaactt gacagaaggt
    28741 gttccagcta aatacttctt gccacttggt ggagatatca aggaagctat cagcaatctt
    28801 gagctcaacc ctccattcaa gaagaaaaac ctcggaaata tcatcgctga aatcttcaaa
    28861 cgtttccgta cgacagaaac ttctgcccta cttgaccgca tgaagaacct cggttaccac
    28921 cactcaactc ttgcaggatt gacagtgggt attgccgata tcccagtcgt tgatgacaag
    28981 gctgaaatca ttgaagaatc acacaaacgt gtcgaacaaa tcaccaaaca attccgtcgt
    29041 ggtatgatca cagacgacga gcgttacaat gctgttacag ctgaatggcg tgctgcccgt
    29101 gaaaaacttg agaaacgctt gattgctaat caagatccta agaacccaat cgttatgatg
    29161 atggactctg gagcccgtgg taacatctca aacttctcac agcttgccgg tatgcgtggt
    29221 ctgatggctg ctccgaatgg acgtatcatg gaattgccaa tcctttcaaa cttccgcgaa
    29281 ggtttgtcgg tattggaaat gttcttctca actcacggtg ctcgtaaagg tatgaccgat
    29341 acggccctta agacagccga ctcaggttac ttgactcgtc gtttggtcga cgttgcccaa
    29401 gacgttatca tccgtgagga cgactgtgga actgaccgtg gtctcttgat ccgttctatc
    29461 gcagaaggaa aagagatgat cgagtctctc gaagagcgtc tcaacggtcg ttacactaag
    29521 aaaactgtta aacatccaga aactggtgca gtgattattg gtccaaatga attgattaca
    29581 gaagacaagg cgcgtgaaat tgtcaatgct ggtgtggaag aagtgactat ccgttctgta
    29641 tttacatgta acactcgtca cggtgtctgc cgtcactgtt acggtatcaa cttggcgact
    29701 ggtgatgcgg ttgaagttgg tgaagcagtt ggtacaatcg ctgcccaatc tatcggggaa
    29761 cctggtacac agcttacaat gcgtaccttc cacacaggtg gggttgcctc aaataccgat
    29821 atcactcagg gtcttcctcg tgtccaagaa atctttgaag cccgcaatcc taaaggggaa
    29881 gcggttatca cagaggttaa aggacaagtt actgctatcg aagaggatgc atcaactcgt
    29941 accaagaaag tctttgttaa gggtgaaact ggcgaaggtg aatatgtcgt tccatttaca
    30001 gctcgtatgc gtgtcgaagt tgggggccaa gtagcgcgtg gtgctgctct gacagaaggt
    30061 tctatccaac caaaacgtct ccttgcagtt cgtgatgtct tgtcagttga aacgtacctt
    30121 ctcggtgaag tacaaaaagt ttaccgtagc caaggggtag aaatcggtga caaacacatc
    30181 gaggtaatgg ttcgtcaaat gatccgtaaa gtccgtgtca tggatccagg tgatacagat
    30241 cttctcatgg gtaccctcat ggatatcaat gactttacag atgccaacaa agatgtcctt
    30301 atcgcaggtg gagttccagc gacaggtcgc ccagtcctta tgggaattac caaagcctca
    30361 cttgaaacaa acagtttctt gtcagcggct tccttccagg aaacaactcg tgtccttact
    30421 gacgcagcta tccgtggtaa gaaagaccat ctccttggac ttaaagaaaa tgttatcatc
    30481 ggtaagatca tcccagctgg tactggtatg gcccgttacc gtaaccttga accacatgct
    30541 gtcaacgaag aagaatacct taatcctcca gtagaggaag aaggaaatga agaaacaaca
    30601 gaagtagttg tggatactgc cgttgaaact gtggaagaaa cagtagaata agagagaatg
    30661 agagaaccct cgggttctct cattctctct tctgaaaact ttctccattt ttccacgaaa
    30721 tgtggtaaaa taaaagaaag aagctgacaa aggagacggg tatggaacaa acattcttta
    30781 tcatcaaacc agatggtgta aaaagagggc tagtgggtga agtgttaaag cgcatcgaac
    30841 aacgtggatt tacaatcgaa aaattggagt ttcgttcaca ggtttcagaa gagttgattg
    30901 accagcacta tcaggacttg gttggtcaga gtttttaccc accgattcgt gaattcatga
    30961 cttcaggtcc agttcttgtg ggtgtcattt ctggtcccaa agtaatcgaa acttggcgga
    31021 ccatgatggg tgcaactcgt ccagaagaag ctttaccagg cactattcga ggtgattttg
    31081 caaaagctgc tggagaaaat gagattatcc aaaatgttgt acatggttca gattcagaag
    31141 agtcagctaa gcgagaaatt gctctttggt tttaagagtg gattggctca atcaattggt
    31201 taaaagctca tttgaataga aagtatagtc aattagttta agacatgacg catgatatca
    31261 aactttttag tttttgatat ggtgcgtttt ttgattaagt gactattagt ttgtctcgct
    31321 ccgttaggtg cgagacaaaa aaaacacccg ctatgcgggt gcgcgtcgaa ggttatacca
    31381 aaaaaactcc aaacgcgata caataaaggt gttcaatcca attgtaaagc gaaaggagaa
    31441 aaatatggca caaaaggttc ttttatcctg ttcttgtttc aattgactat atttctatac
    31501 tttttcttcc tttctctgtg aaaatcgcaa gtttttccca ccttttctca atactgaaac
    31561 aacatctttc cagaacttca ataatttctt tttgtgttaa atcattattt aactaaatct
    31621 ttggaggcaa tacgttttca aagctacttt tttaccatag gttttgagcc ttcaacacag
    31681 attactaagc caaaaatgct ctaaattttt gaaattctaa caaattccac tcaactttat
    31741 catcatcttt gactttcaca atatcttcct caaaataaca tggaataaag atagttttct
    31801 tttaaaacgg ttgtaggagg aaaatctctc ctacactatt attgattctg agagaaaatt
    31861 gcaaaatatc atctaccaaa ctttcaatta ttaagatttt ttgcagttta tctgaattag
    31921 aatcgcaagg cttctcaata atataattgt cgaatttttt cattctaact tcctctccat
    31981 tatttcttcc tttatcaaga aaaagttacc tcaatattaa taaagtacaa ttctaaatta
    32041 actccaattc ttgattcatg ataatatttc agtaactcat cgtgcaatta aaaacaagga
    32101 tttttatttc agatgatcca tcgtgaacac ttcatcagcc atctcccaaa ttgccggatt
    32161 atgtgttgcg ataatgatta gcctattatc atctcgaaga gatagcaaaa tctccataat
    32221 caactgagag gttgctgggt ctattgaagc tgttggctca tctgccagaa taaagggtgg
    32281 attctttaag ataatttttg ccaaggcaac ccgttgcgat tctccgcccg ataactcaaa
    32341 gatgcgctta tctaggtcaa gataagccag gccgacctgt tctaaagcct gcttctgcct
    32401 caaccgctgt tccgaccgac tcaacttttg accaatgaga cctagcttaa ggttttcttc
    32461 aatactttgg ttttcaatta agccaaagtt ctggaagagg tagcccaatt cgtgacggaa
    32521 aaaatcactc gatttataat tggccaagtc tttacctcgg taaaaaatcg tcccatcata
    32581 aggttctaat ttcccaatca tgttcatcaa ggttgttttt ccgctaccac ttgaaccaat
    32641 taaggcatag acttttccag cctcaaatgt cattgaaaga ttcgaaaata actctcgttc
    32701 tccaaaagat ttactcacct gtttaagttc aatcatatta acctcccttc aaaacaagaa
    32761 tggacatctt gttttctttc tgcatttgga catgtaactg taaaagagat agaccagtaa
    32821 agagtaacaa gactaagaaa gcaactccta tctctacctg aagaaataca ctcgcaacaa
    32881 atcccagtaa aaacacaccc agttgagcaa agagataagt gcgatggatt tctaagaacc
    32941 tgagacctgc aatgcgtttg ataaaaatgg cacgtctaaa ttcttcaaag tagagcctat
    33001 tcatagtgtt aaacaacaag attgaagttg caatcccaag cacagctcct gctagcatta
    33061 cccaacgttc cctctggata ttatccaata atgtgatgta gttgtggtaa cctgtttgca
    33121 tttctgagac ccaattttca atgccttgtc tctggataag ctcctgggca tcagacaatt
    33181 gattaaagag aacgtagttc tgtactgcgt ctatccaaaa caaaatggac tgtggaccag
    33241 ttgattgggg tgttataaca atgatgattg gatctttcaa aaactgctgg taagaaatag
    33301 gggtcgtatt atacacaaaa cgatcctgac ctgattctaa ataacctacc gtagcagtca
    33361 tttgctgtcg ttcatcttga ctagacatgc gactggttaa gtcgtcttca aaaacagatt
    33421 tataatgttc ttcctctgaa cggaggtgtt caggcagcaa taagacaaac tcccctacat
    33481 taaggtgatt catcttttgc tcaatagttg tatctacagg aatgttttga cgctccaagt
    33541 attgcggtgt gacgataagg acattgccat tcgggttgta atcgtgccat tctgtagagg
    33601 ttatcaagtt tttggaggaa gccatgccat tttgcaaagt acggtcaatt aactggtgtc
    33661 tagataagaa agccttttgt tctgaaacag ccagatccat caattgatac caagttctga
    33721 gtttcctttg agcttgttca tcaaaaccag gactcgttcc ttcacggctg attgataggg
    33781 taatcagttg cctttcctga ctccaagcct cttgtccaat ccgatgctgt tgccaggctt
    33841 gggaatactt taagctactc cctattccca gcgttacaat aataatcgct aatagttgac
    33901 cgattaaaat caaactaatg attcctctga caggcacttg cccttttata atctgcatca
    33961 ggtgtatctt ttttatccca actgcgaaga gttgagcgaa aaacagggag atcgacaaca
    34021 agatgagatt ataactgagc aagcctgctc ctatggtcat tagggattgc gttggaagcg
    34081 acagaatttt ttgcataaga atggcgagca cgccagctaa actgaaacca acagctatcc
    34141 ctttcaaatc ctcaccaact ggtctaagga aaatggacca ccgtttctct cctgaaatga
    34201 ggcgaatgcc cgacgaccgt aattggctaa tttgactaat taaagtcagt gctacaaagg
    34261 ttaagatgaa aatcaataga ctgattaact gaaatccgtt actaaagata gccattaacg
    34321 tagatagttt agatggaatt gtcaggttca tgttagtcaa gccaagttga cttagctcct
    34381 cccttagcaa gtgaatatct aggtgtccat cgaatacaaa atagtttgtt tcaacactac
    34441 tactttgagc atcttctagt tttttttcct gaagcccatc aggcaacttt ccgtccccaa
    34501 aggtcgtata agaaaaaact gtagtacctt ctgagttaga atcttggtgt tgaatcgcaa
    34561 taaagctatc tgtttcttct gctagttttt ccaagcgtgt agcaacatgc tgaaaagttg
    34621 tttcagggga agaatcacgt acaacaacgt tggggtaata atgggagaca tatgtatcag
    34681 tccaaatggt aaatacccaa acaaagaaca aactagcaag caggttggat atgagtataa
    34741 ataatttttt catagtgcat tccttattct aaaacagagg gcagaaatac ccctgccctc
    34801 tataaactaa caagcttact aaactattga taaaagaatc ttcttcaatt attctttacc
    34861 atcccgcatc aaatctaaat tttaacatat t
//
LOCUS       DAVLSL010000004        34492 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLSL010000004 DAVLSL010000000
VERSION     DAVLSL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.004"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            42..2021
                     /locus_tag="VXN30_000145"
     CDS             42..2021
                     /locus_tag="VXN30_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1356827.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            2088..3584
                     /locus_tag="VXN30_000146"
     CDS             2088..3584
                     /locus_tag="VXN30_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1356828.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            3584..4510
                     /locus_tag="VXN30_000147"
     CDS             3584..4510
                     /locus_tag="VXN30_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1356829.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            4519..5586
                     /locus_tag="VXN30_000148"
     CDS             4519..5586
                     /locus_tag="VXN30_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356830.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFSEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            5597..6523
                     /locus_tag="VXN30_000149"
     CDS             5597..6523
                     /locus_tag="VXN30_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356831.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            7013..7744
                     /locus_tag="VXN30_000150"
     CDS             7013..7744
                     /locus_tag="VXN30_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1356832.1"
                     /translation="MGILLTSLISLHQLKADKKRDVFRIGISQFITHQSLDATREGFV
                     DELAKQGYVEGENIEIDLQNAQGEQRNLKTISQQLAESSDVVLAIARPSAQSEPNSVV
                     QKDEAKRLLKEKGFTVVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQ
                     VSIKHKVPVFGGSTEMIAVGGLYNYGTNYEELGRQTARMLIHVLKGEKPENIAVELPE
                     KLELHTNQEMADALGIDISKLEGKE"
     gene            7756..8649
                     /locus_tag="VXN30_000151"
     CDS             7756..8649
                     /locus_tag="VXN30_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1356833.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIVEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            8662..9465
                     /locus_tag="VXN30_000152"
     CDS             8662..9465
                     /locus_tag="VXN30_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1356834.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(9498..10208)
                     /gene="treR"
                     /locus_tag="VXN30_000153"
     CDS             complement(9498..10208)
                     /gene="treR"
                     /locus_tag="VXN30_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1356835.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENSLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            10393..12360
                     /gene="treP"
                     /locus_tag="VXN30_000154"
     CDS             10393..12360
                     /gene="treP"
                     /locus_tag="VXN30_000154"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1356836.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVSHELVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETLVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            12538..14163
                     /gene="treC"
                     /locus_tag="VXN30_000155"
     CDS             12538..14163
                     /gene="treC"
                     /locus_tag="VXN30_000155"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1356837.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            14419..14667
                     /locus_tag="VXN30_000156"
     CDS             14419..14667
                     /locus_tag="VXN30_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1356838.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            14874..15668
                     /gene="racE"
                     /locus_tag="VXN30_000157"
     CDS             14874..15668
                     /gene="racE"
                     /locus_tag="VXN30_000157"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1356839.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            15665..16636
                     /locus_tag="VXN30_000158"
     CDS             15665..16636
                     /locus_tag="VXN30_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1356840.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            16612..17133
                     /locus_tag="VXN30_000159"
     CDS             16612..17133
                     /locus_tag="VXN30_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1356841.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            17130..17591
                     /locus_tag="VXN30_000160"
     CDS             17130..17591
                     /locus_tag="VXN30_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1356842.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            17582..18322
                     /gene="xerD"
                     /locus_tag="VXN30_000161"
     CDS             17582..18322
                     /gene="xerD"
                     /locus_tag="VXN30_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1356843.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            18322..19050
                     /locus_tag="VXN30_000162"
     CDS             18322..19050
                     /locus_tag="VXN30_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1356844.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            19069..19638
                     /locus_tag="VXN30_000163"
     CDS             19069..19638
                     /locus_tag="VXN30_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1356845.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            19625..20347
                     /locus_tag="VXN30_000164"
     CDS             19625..20347
                     /locus_tag="VXN30_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1356846.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            20347..20589
                     /gene="yidD"
                     /locus_tag="VXN30_000165"
     CDS             20347..20589
                     /gene="yidD"
                     /locus_tag="VXN30_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1356847.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(20814..21779)
                     /locus_tag="VXN30_000166"
     CDS             complement(20814..21779)
                     /locus_tag="VXN30_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1356848.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(21875..22627)
                     /locus_tag="VXN30_000167"
     CDS             complement(21875..22627)
                     /locus_tag="VXN30_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1356849.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(22624..23580)
                     /locus_tag="VXN30_000168"
     CDS             complement(22624..23580)
                     /locus_tag="VXN30_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1356850.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYILLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILIGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(23570..24529)
                     /locus_tag="VXN30_000169"
     CDS             complement(23570..24529)
                     /locus_tag="VXN30_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1356851.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(24952..25653)
                     /gene="galE"
                     /locus_tag="VXN30_000170"
     CDS             complement(24952..25653)
                     /gene="galE"
                     /locus_tag="VXN30_000170"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1356852.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            26054..27118
                     /gene="pepA"
                     /locus_tag="VXN30_000171"
     CDS             26054..27118
                     /gene="pepA"
                     /locus_tag="VXN30_000171"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1356853.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            27140..27436
                     /locus_tag="VXN30_000172"
     CDS             27140..27436
                     /locus_tag="VXN30_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1356854.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            28118..28561
                     /locus_tag="VXN30_000173"
     CDS             28118..28561
                     /locus_tag="VXN30_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1356855.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            28565..29329
                     /locus_tag="VXN30_000174"
     CDS             28565..29329
                     /locus_tag="VXN30_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1356856.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            29344..30072
                     /locus_tag="VXN30_000175"
     CDS             29344..30072
                     /locus_tag="VXN30_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356857.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSIHD"
     gene            30065..31585
                     /locus_tag="VXN30_000176"
     CDS             30065..31585
                     /locus_tag="VXN30_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1356858.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALMAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            31930..32736
                     /locus_tag="VXN30_000177"
     CDS             31930..32736
                     /locus_tag="VXN30_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1356859.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(32902..33438)
                     /locus_tag="VXN30_000178"
     CDS             complement(32902..33438)
                     /locus_tag="VXN30_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1356860.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            33571..34461
                     /locus_tag="VXN30_000179"
     CDS             33571..34461
                     /locus_tag="VXN30_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1356861.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
BASE COUNT         9960 a         6445 c         7920 g        10167 t
ORIGIN      
        1 tggttgtcaa tctataattc acataaacag aggagtagaa aatgaaaaaa aatcgtgtat
       61 ttgctacagc aggtcttgtt ttattagcag caggtgtact tgcagcatgc agttcttcaa
      121 aatcatctga ttcatcagcc cctaaagctt atggctatgt ttatacagca gacccagaaa
      181 ccttggacta cctgatttca agtaaaaata gtacaacagt agtgacttca aatgggattg
      241 atggtttatt cactaacgat aattacggta atcttgctcc tgcagttgca gaggattggg
      301 aagtctctaa ggatggtttg acctacactt ataagattcg taaaggggtt aaatggttta
      361 cctctgatgg agaagaatat gcagaggtga cggctaaaga tttcgtgaac ggtttaaaac
      421 acgcagcaga taaaaaatca gaagctatgt atttagctga aaattcggtt aaaggcttgg
      481 cagattatct atcaggaact tcaacagatt tttcaacagt tggtgtcaag gcggttgatg
      541 attatacgtt acaatacact ttgaaccagc ctgaaccgtt ctggaactct aagttgacct
      601 attctatttt ctggcctctg aatgaagaat tcgaaacatc aaaaggaagc gattttgcta
      661 aaccaacaga tccgacatcc ttgctttata atggtccatt cttgttgaaa gggttgactg
      721 caaaatcttc tgtagagttt gtaaaaaatg agcaatattg ggataaagaa aatgtccacc
      781 tagatactat caatctagct tactatgatg gatcagatca ggagtcgcta gagcgtaact
      841 tcactagtgg agcctatagt tatgcccgtc tttaccctac cagctccaac tattctaagg
      901 ttgcagaaga atacaaggac aatatctatt acacacaatc aggctctggg attgctggtc
      961 tgggtgtgaa tattgatcgc caaagttaca actatacttc taaaactaca gattcagaga
     1021 aagtagctac taagaaggca ttgcttaaca aagatttccg tcaagccttg aattttgctc
     1081 ttgatcgctc agcttactca gctcaaatca atggtaaaga tggagcagct ttagcagttc
     1141 gtaatttatt tgtaaaacca gactttgttt cagctggtga gaagaccttt ggtgatttag
     1201 tcgctgctca acttcctgct tatggtgatg agtggaaagg tgtgaattta gctgatgggc
     1261 aggatggttt attcaatgct gacaaggcca aggcagagtt tgcgaaagct aagaaagctt
     1321 tagaagcaga cggcgttcag tttcctattc atctggacgt tccagtagac caagcatcaa
     1381 aaaactatat atctcgtatt cagtccttta aacaatctgt agaaacagtt cttggtgttg
     1441 aaaatgtcgt tgttgatatt caacaaatga caagtgatga attccttaat attacttact
     1501 atgctgccaa tgcttcatct gaggattggg atgtatcggg aggagtttca tgggggccag
     1561 actatcaaga cccatctact tacctggata ttttaaaaac aactagcagt gaaactacaa
     1621 aaacatattt aggatttgat aatccaaata gcccttcagt agttcaagtt ggtttgaaag
     1681 aatacgataa attagttgat gaagctgcca aagagacaag cgacttgaat gtccgttatg
     1741 aaaaatatgc agcggctcaa gcatggttga cagatagttc actctttatt cctgctatgg
     1801 cttcttctgg tgcagcacca gtgctttcac gaattgttcc atttactgga gcttctgcgc
     1861 aaacaggctc taaggggtca gatgtttact tcaaatattt gaaattacaa gataaagcgg
     1921 tgactaagga agagtatgaa aaagctcgtg aaaaatggtt gaaagaaaaa gctgaatcaa
     1981 atgagaaagc tcaaaaagaa ttggcaagtc atgtgaagta aataatttct aatagaactt
     2041 tctctccatg aggagaaggt tcttttggga tttttaaagg aaataatatg aaaaaatata
     2101 tttttatgcg tgttttgcgg tcattggttt cgattttctt agtaacgact ttgacctaca
     2161 cgattatcta taccttggtt cctcgaaaat tgattttcaa gcaggatcct aactataata
     2221 aaattgcgac aacggctgat aaacgtgata actatgaaaa tactgtgttt gagcgtatgg
     2281 gctacattga gtattacgat actaaagagt tgcaagaaaa ggcaagtagc atggattctt
     2341 ctgtaacagt agaagcaaat gcgaccaata aagctattta tgaaaagtac atcaatcaat
     2401 taggtcatgg ttggactttg ggagaattta ctgaaagtgg tcaattctat gctactcgtg
     2461 aaattccaat ttttgaacgt gtttttcact tctatgctaa cttgattgac attgaccata
     2521 caaataaaat ccaagaccct gaaaatccag acttgaaacg ctaccttcgt tttgaaaatg
     2581 atccagctat cggatggtca ttggtcggtt caggaactaa acataaatat ctcttgtact
     2641 ttaacagtca gttcccattt gttcatcaaa actttgtgaa cttgaattta ggtgactctt
     2701 acccaaccta tgctaataca ccagttcttc aggttattac tcaaggtcaa ggacaaacca
     2761 aaactgccca agttcagttc ccaacaggta agaaaacgtc ttctgtaaat atttactcaa
     2821 gaacctacaa gtcacctagt caggctgact ctcgtgaagt agctagctat gggaaagatg
     2881 atccttatac agcgactgaa agtaattacc aatatccatc tatgattgtc agctctgcta
     2941 ttactggttt gattggtttg gttcttgcct atgctcttgc cgtgccactt ggttcagcca
     3001 tggctcgttt caagaacact tggattgata gcctctcaac aggggctttg accttcttgc
     3061 ttgctcttcc aacgattgcc ttggtttaca tcgttcgatt gattggatca tctatttccc
     3121 ttccagattc attccctatc ttgggagctg gagattggcg ttcttacgtt ttaccagcag
     3181 tcatccttgg tttgttgggt gctcctggta cagccatttg gattcgtcgt tacatgattg
     3241 acttgcaatc tcaagacttt gttcgtttcg ctcgtgcaaa aggtttgtct gaaaaagaaa
     3301 tttcaaacaa acacatcttt aaaaatgcca tggttccgct ggtttcagga attcctggtg
     3361 ccattattgg ggttatcggt ggtgcaaccc ttactgaaac agtcttcgcc ttcccaggta
     3421 tgggtaaaat gttgattgac tctgtaaaag catctaataa ctctatggtc gttggtcttg
     3481 tcttcatctt tacatgtatt tctatcttct cacgtctttt gggagatatt tggatgacta
     3541 ttattgaccc acgtattaaa ttgactgaga aaggaggcaa ataatgtcta caatcgataa
     3601 agaaaaattt cagtttgtaa aacgtgacga ttttgcctct gaaactattg atgcgccagc
     3661 atattcttac tggaaatcag tgtttaaaca atttatgaag aaaaaatcaa ctgtagtcat
     3721 gttgggaatc ttggtagcca tcattttgat aagtttcatc tacccaatgt tttctaagtt
     3781 tgatttcaat gatgtcagca aggtaaacga ctttagtgtt cgttatatca agccaaatgc
     3841 ggagcattgg ttcggtactg acagtaacgg taaatcgctc tttgacggtg tctggttcgg
     3901 agctcgtaac tccatcctca tttctgtgat tgcgacagtg attaacttgg ttatcggtgt
     3961 ttttgtcggt ggtatttggg gtatttcaaa atcagttgac cgtgtcatga tggaagttta
     4021 caacgtcatc tcaaacatcc cacctctttt gattgttatt gtcttaactt actcaatcgg
     4081 agctggattc tggaatctga tttttgccat gagcgtaaca acatggattg gtattgcctt
     4141 catgatccgt gtgcaaatct tgcgctatcg tgacttggaa tacaacttgg cgtcacgtac
     4201 tttgggaaca ccaaccttga agattgttgc caaaaatatc atgcctcaat tggtatctgt
     4261 tattgtgaca accatgactc aaatgcttcc aagctttatc tcatacgaag ccttcttgtc
     4321 tttcttcggt cttggattac cgattacagt gccaagtttg ggtcgtttga tttcggatta
     4381 ttcacaaaac gtaacaacca atgcttactt gttctggatt ccattgacaa cccttgtctt
     4441 ggtatccttg tcccttttcg tagttggtca aaacttagcg gatgctagtg atccacgtac
     4501 acatagatag gagtagaaat gacaaaagaa aaaaatgtaa ttttgactgc tcgcgatatt
     4561 gtcgtggaat ttgacgttcg tgacaaagta ttgacagcca ttcgcggcgt ttcccttgaa
     4621 ctagtcgaag gagaagtatt agccttggta ggtgagtcag gatcaggtaa atctgttttg
     4681 acaaagacct tcacaggtat gctcgaagaa aatggtcgta ttgcccaagg tagtattgac
     4741 taccgtggtc aggacttgac agctttatct tctcacaagg attgggaaca aattcgtggt
     4801 gctaagattg cgactatctt ccaggaccca atgactagtt tggaccccat taaaacaatt
     4861 ggtagtcaga ttacagaagt tattgtaaaa caccaaggaa aaacagctaa agaagcgaaa
     4921 gaattggcca ttgactacat gaataaggtt ggcattccag acgcagatag acgttttagt
     4981 gaatacccat tccaatattc tggaggaatg cgtcaacgta tcgttattgc gattgccctt
     5041 gcctgccgac ctgatgtctt gatctgtgat gagccaacaa ctgccttgga tgtgactatt
     5101 caagctcaga ttattgattt gctaaaatct ttacaaaacg agtatcattt cacaacaatc
     5161 tttattaccc acgaccttgg tgtggtggca agtattgcgg ataaggtagc ggttatgtat
     5221 gcaggagaaa tcgttgagta tggaacggtt gaggaagtct tctatgaccc tcgccatcca
     5281 tatacatgga gtctcttgtc tagcttgcct cagcttgctg atgataaagg ggaactttac
     5341 tcaatcccag gaacacctcc gtcactttat actgacctga aaggggatgc ttttgccttg
     5401 cgttctgact acgcaatgca gattgacttc gaacaaaaag ctcctcaatt ctcagtatca
     5461 gagacacatt gggctaaaac ttggcttctt catgaggatg ctccaaaagt agaaaaacca
     5521 gctgtgattg caaatctcca tgataagatc cgtgaaaaaa tgggatttgc ccatctggct
     5581 gactaggagg aaggaaatgt ctgaaaaatt agtagaaatc aaagatttag aaatttcctt
     5641 cggtgaagga agtaagaagt ttgtcgcggt taaaaatgct aacttcttta tcaacaaggg
     5701 agaaactttc tcgcttgtag gtgagtccgg tagtgggaaa acaactattg gtcgtgctat
     5761 catcggtcta aatgatacaa gtaatggaga tatcattttt gatggtcaaa agattaatgg
     5821 taagaaatcg cgtgaacaag ctgcggaatt gattcgtcga atccagatga ttttccaaga
     5881 ccctgccgca agtttgaatg aacgtgcgac tgttgattat attatttctg aaggtcttta
     5941 caatcaccgt ttatttaagg atgaagaaga acgtaaagag aaagttcaaa atattatccg
     6001 tgaagtaggt cttcttgctg agcacttgac tcgttaccct catgaattct caggcggtca
     6061 acgtcaacgt atcggtattg cccgtgcctt ggtcatgcaa ccagactttg ttattgcaga
     6121 tgagccaatt tcagccttgg acgtttctgt acgtgcccaa gtcttgaact tgctcaaaaa
     6181 attccaaaaa gagctcggcc tgacctatct cttcatcgcc catgacttgt cggttgttcg
     6241 ctttatttca gatcgtatcg cagttattta caagggtgtt attgtagagg ttgcagaaac
     6301 agaagaattg tttaacaatc caattcaccc atatactcaa gccttgcttt cagcggtacc
     6361 aatcccagat ccaatcttgg aacgtaagaa ggtcttgaag gtttacgacc caagtcaaca
     6421 cgactatgag actgataagc catctatggt agaaatccgt ccaggtcact atgtttgggc
     6481 gaaccaagcc gaattggcac gttatcaaaa aggactaaac tagtaatggt tttataattt
     6541 ccatgtcaat ttttatggaa attataaaac tttttttgtt ggactgattt gaaaaacttt
     6601 gaaagcttcc tgaaagaatt tttgaaaaat tttaaaaatt atgaaatgtt attgacaggt
     6661 gatgaaaaag gtggtaggac aggaagcatc aaaaaagaaa gcagaaaaag aaatgaataa
     6721 gagacaagcc gtaactatga atcaaaactt ttactttagc tactttagat agtgtttgta
     6781 gacatgtcac catggatgca aacagttcaa gcaatttgtt tgtatctatg tggctaagat
     6841 ttttgattgt ggtccatata gatgaatatc taaatggact agctaagttg taacttgtta
     6901 ggttatttca agtatccgtt tagatagtat ctaggcggtt ttttgtttta tcttttctga
     6961 gaaggagttt cattatgaaa gttgttcgaa aatcactagc cccctcttgg tagtggggat
     7021 cctcttgact tctctgatca gtttgcatca gttgaaggca gataagaaaa gagatgtctt
     7081 tcgtatcggt atttcgcagt ttattaccca ccagtcctta gacgctacta gagaaggatt
     7141 tgtggatgag ctggccaaac aaggctatgt tgagggggaa aatattgaga ttgatttgca
     7201 aaatgcacag ggagaacaaa gaaatctaaa aacgatttcc cagcaactag cagaatccag
     7261 cgatgtcgtt ctagccatcg caaggccttc tgctcagagc gagcccaatt cggttgttca
     7321 aaaggatgaa gctaagcgac ttctgaaaga aaaaggcttt accgttgttg aaaaaacaat
     7381 cttggacagt aataacgtca aggcggcagc agagagtttg atggcagagg tggatatggt
     7441 ttttgtccca actgataata tcatttcgtt aaccatggaa acagttaagc aggtttctat
     7501 taaacacaag gttccagtat tcggtggttc aacagaaatg attgctgttg gtggcttgta
     7561 taactacgga accaattatg aagaattggg aagacagaca gctcgcatgc tgattcatgt
     7621 tttaaaaggt gagaaaccag aaaatatagc agttgagttg cctgaaaaac tggaattgca
     7681 tactaatcaa gaaatggcag atgcattggg aattgatatt agtaagttag aaggcaagga
     7741 ataaggagga ttaagatgaa ttttgtgtta tctagtttat cagaaggttt gttgtggtcg
     7801 attgtggcga ttggggtcta cttgactttc cgtattttgg atattgcgga tatgactgct
     7861 gagggagcct ttcctctggg ggcagcagtc gttgtatcac agatacaggc aggggcaaat
     7921 ccttggcttg cgactttact tgctttgctg gcaggtatgg tagcaggtct tgtatcagga
     7981 atgctccaca ccaagatgaa aatcccagct ctcttgacag ggattgtgac cttgacaggg
     8041 ctctattcaa tcaatattaa aatcatgggc agtgtaccca atctttcctt gggagattct
     8101 gcaaccgtct ttaaacagtt ggcgagcttg ggtctgacaa atgaaggaac tgttttctca
     8161 cttagtttgg cctgtttttt acttgtttgt ttggtcttga cccttttgat gaaaacagag
     8221 attggcttgg tcttgcgttc gactggggat aatatcccga tgagtgaggc caatggagtc
     8281 aatgtagaca ccatgaagat tgttggttac atgatttcaa acggtttgat tgctctatgt
     8341 ggttccttgt ttgcccaaaa tgatggattt tcagatgtga cttctgggac aggaaccatt
     8401 gttgttggtt tgagctcggt gattattgtg gaagtcttga tacacgactt gaccattgga
     8461 ggccgcttat tatccatcgg aatcggtgct attgtttacc gtttgattat tttaaatatc
     8521 tatgaaattc caaatctaga tcaaaacttg gtacgtctct ttaatgcaat cttgctcgct
     8581 ttggttttat ttgcaccaga attgcaaaag agattaaaga ttcgtggtct gaaattgaga
     8641 aatgaatagg aggagaaagt tatggcaagt ttgttaacac ttgaaaatat tcacaagaca
     8701 tttgaagcag ggacggtcaa tgagaaccat gtcctcaaag gattagattt agaggttgaa
     8761 gagggagatt ttatctctgt gattggtgga aatggagcag gaaaatccac tttgatgaat
     8821 atcttggctg gcaatctatc ggtggacgaa ggggaccttc tactggcagg gaaatccatt
     8881 aaaaatctga gtgtcagaaa gagagccaag gatattgccc gtgtttttca agatcctaag
     8941 atgggaacag cttctcgttt gacgattgag gagaatatgg ctattgcctt gagacgcggg
     9001 cagaagagag ggcttggttg gggcgtgaag gagaaggata gaatccagtt ccaagaggcc
     9061 ttgaaggagt tgaatattgg tcttgaaaac cgcttaaaag tagatactca atatctctca
     9121 ggaggacaaa gacaggcttt gactctagtc atgacagatc tgatgaaacc aaaactttta
     9181 cttttggatg aacatactgc ggcacttgac ccgaaaacca gtcaaatggt gatggacttg
     9241 acgcaaaaga ttgtggagca ccatcagtgg acgactttga tgattaccca tgatatgaat
     9301 catgcgattg agtacggcaa tcgtctcatt atgctctatc aaggcaagat agtggtggat
     9361 gtcaagggag aagagaaaaa gcatctgaca gttgaagacc ttatgcatct cttccagaaa
     9421 aatagtggcc aaagtctggt cagtgatgaa ttagttttgg gataaagaaa aaggctgaga
     9481 tctggtgtct cagtctttta tttcttgcgt tttgcaaaat ctacaaatct aaatttttct
     9541 aacttatggc gactttcggt aaactgaaat tggcgtccgt cttggagata gacttttgat
     9601 ttaatcgaaa cgacataagg gtctttgcca atgtccatga gaatcttgtc tcggtcgctt
     9661 gagtggtcaa tggtgatttc cttctgagca taatcaataa ggagtttgag gctattttct
     9721 atataagaat agatagactg ctcagcaatt tggcgagtga gatttgggat tagttccata
     9781 tccagatagt ccgtatccag aacggatacc agatcatcca ccacacgctg gcggaccacc
     9841 ttccaaacca tccgaaactc tgggaaaccg gttatcagtg aggatttttt atcaataata
     9901 atcttgtcca gactgaccac gttggtttta gagcgcagtc caagttcttt aactagttct
     9961 tggtagctgg ttaggttgga tacagggaaa ttgacggttt cttctttgac gacttgagaa
    10021 ccttgccctc ttatcttttt gatcaatcct tcctcttgga ggagagacag ggcctttcgg
    10081 acggtatcac gactcacttg atattgctcc ataaggtcgt gctcgctagg aagaaaatct
    10141 ccgacagcgt aagtctcgtt ttgaatggtt tcttggattt gcttaaataa ttgttggtat
    10201 tttttcattt tgctttctta aataggtttt tagtctagtt taatgaacac ctgtatctat
    10261 tttaccatac aagtgctaga aaatttacaa aaaaacaccc tttttatacc aaacaagttg
    10321 cagacaagtt tggtatcgtt tacaatatac ttatcaaatc aaacttgctt tgacaagtat
    10381 aaggagaatc aaatgggaaa atttgaacaa gaagccaaag atttgcttca ggcaattgga
    10441 ggtaaagaga atgtgactgc cgtcacccac tgtgcgacac ggatgcggtt tgttttagga
    10501 gatgataaga aggctaatgt taaagctatc gagtcaattc cagctgttaa aggaaccttt
    10561 acaaatgcag gtcaatttca ggtaatcatt ggaaatgacg tgcccatctt ttataatgat
    10621 tttacagccg tttcaggtat tgagggtgtt tccaaagaag cagccaagtc tgcagctaag
    10681 agtaatcaaa acgtggtcca aggtgttatg accactctgg cggaaatctt tactccgatt
    10741 attccagcct tgattgttgg tggattgatc ctcggtttcc gtaatgtctt ggaaggtgtc
    10801 cattggtcga tgttggatgg caagaccatc acagaatcct ctcagttttg ggcaggggtt
    10861 aatcacttcc tctggttgcc tggtgaagct atcttccagt tcttaccagt agggattact
    10921 tggtctgttt ctcgtaagat gggaaccagc caaattttgg gaattgttct cggaatctgt
    10981 ttggtatcgc ctcagttgct caatgcctat gcggttgctt caacgccagc agctgatatc
    11041 gcagcaaact gggtttggaa ttttggttat tttactgtta atcgtattgg ttaccaagcc
    11101 caagttatcc cagccttgct tgcaggtttg agtctgtctt atcttgaaat tttttggcgc
    11161 aaacatatcc cagaagtcat ttccatgatt tttgtacctt ttttgtcatt gattccagcc
    11221 ttgattttgg ctcatactgt cttggggcca atcggttgga caattggaca aggactttca
    11281 tcagttgtat tggcaggttt aacgggtcct gtaaaatggc tcttcggtgc aatttttggt
    11341 gccctctacg ctccatttgt catcacaggt ctgcaccaca tgaccaatgc cattgataca
    11401 caattgattg cggatgctgg tggcactgcc ctctggccta tgattgctct ttctaatatt
    11461 gctcaaggct cagccgtgtt tgcctattat ttcatgcatc gccatgatga gcgtgaggct
    11521 caggtttcac ttcctgcaac catttcagcc tatctcggtg ttacagaacc agctcttttt
    11581 ggggttaacg taaaatatat ttatccattt gttgctggga tgactggttc agcccttgca
    11641 ggcatgttat ccgttacttt taatgtaact gcggcttcta ttggtatcgg tggtttgcca
    11701 ggtattctct ctattcaacc tcaatacatg ctgccatttg caggaactat gctagttgcg
    11761 attgttgttc caatgctctt gactttcttc ttccgcaagg ctggtctctt tacaaaaaca
    11821 gagggcgata cgaacttgca ggcagaattc gttgctcaag aagaagcaga atttgtgagc
    11881 catgaactag tagaacttac ttcggtagaa attatcagcc cactaactgg ccaagtgaaa
    11941 gaattgagtc aagcgacgga tcctgttttt gcatcaggtg tcatggggca aggtctggtc
    12001 attgaaccaa gccaaggtga gttgacctct ccagttaatg gaacagtgac ggttcttttc
    12061 cctaccaagc atgccatcgg cattgtctct gacgagggag ttgaattgct catccacatc
    12121 ggtatggata cagtaggtct tgatggcaaa ggttttgaaa gtcttgtagt ccaaggagat
    12181 cacgttatag ttggccagca actgattcgt tttgatatgg atgtcattaa ggctgcaggt
    12241 ctggtgacag aaactcttgt tatcatcacc aaccaagatg cttatacagc gactattccc
    12301 ggaacttatc cgacaacgat ccaagctgga gcatctctca tggtcgctac acgaatctaa
    12361 tactcttcga aaatctcttc aaaccacgtc agctctatct gcaacctcaa aatagtgttt
    12421 tgagcaacct gtggctagct tcctagtttg ttctttgatt ttcattgagt ataattgaaa
    12481 tagcacaaat agggacggaa gactgcttcc tcccttttat cagaaaagga gaaacagatg
    12541 acacttgata aaggaaaagt agtctatcaa atctatccaa aatcttacaa agacaccact
    12601 gaaaacggta ttggggattt ccgtgggatt atcggaaaaa ttccctatct agccaaacta
    12661 ggtgtggata tggtctggct caatccattc tatccaagtc ctcaacggga taatggttac
    12721 gatatttcag attatatggc agtggatcct ctttttggtg atatggctga ttttgaggaa
    12781 atggtgtgta tcggtaaaga gcacaagatt gactttatgc tggacatggt acttaatcat
    12841 tgttcgacag agcatgaatg gtttcagaaa gccctagctg gtgataagta ttatcaagac
    12901 tttttcttca tccaagacca accaacagat tggcagtcta agtttggtgg ttctgcatgg
    12961 gcgcctttcg gggatactgg gaaatactat cttcacctat ttgatgagac ccaggctgac
    13021 cttaactggc gcaatcccaa tgtccgtaag gagcttttca aggttgttaa tttctggcgc
    13081 gacaagggtg tcaaaggttt tcgatttgat gtgatcaatt tgatcggtaa ggacgaggtt
    13141 tcagtggact gtcctgaaaa tgaagggaag ccagcttata cagacaaacc catcgttcat
    13201 aactatttgc gtatgatgaa ccaagccact tttggatctg acgatagctt tatgacagtt
    13261 ggggaaatgt cttctaccac catggaaaac tgtgtccttt attcgtcgcc tgatcgtcag
    13321 gaattatcca tgacctttaa ttttcatcac ctcaaggtgg actataagga tggacaaaag
    13381 tggaccttgg ctccctttga ttttgaagag ttgaagagtc tttaccatag ttggggtaag
    13441 gaaatgagtg ataaagacgg ttggagtgcc ctcttttgga acaatcacga tcaaccacgt
    13501 gccttgaacc gttttgtcga tattcaaaac ttccgcaagg aaggagccac catgctagcg
    13561 gccagcattc acctgtcacg tgggacacct tatatctaca tgggtgagga aatcgggatg
    13621 attgacccag actatgattc tatggctgat tatgtggatg tcgaatcgct taatgcctat
    13681 cagatgctct tagaagaagg aaagagtcag caagaagcct tccagattat tcaagctaag
    13741 tcgcgtgata attcacgcat tcccatgcag tgggatgctt cggaaaatgc aggattttca
    13801 acaggcactc cttggttgaa agctggtaaa tcctataaag acatcaatgt agaaaatgaa
    13861 atccagggtc cgatttttac cttctatcaa gacttgattc gactgcgtaa ggaaatgcca
    13921 atcatttcag aaggaagtta caaaccagcc tttgaagata gcaaacaagt ctacgctttt
    13981 gaacgccagt ttgaggatca aaagttacta gtgctcaata acttttatgc taaagaagtg
    14041 gaaatcgact tgccagcagt ctaccaaaat ggacagattt tgatatcaaa ctatgaagat
    14101 gcagaagtgt ctgagaaaat tctactcaaa ccctatcaaa cactggctat ttatgtaaat
    14161 taatactctt cgcaaatctc ttcaaaccgt gtcagtttta tctgcaacct caaagcggtg
    14221 ctttgagcaa cctgtggcta gcttcctagt ttgctctttg attttcattg agtataaact
    14281 tagcaaggag acctcttttt cagaaaagca gggttctttt tgctgtctat tccatccctg
    14341 aaagtgtaga acaaactggt cagggtggct agtttgtggt ataatgaaag ggactcaaaa
    14401 agaaacagga gaaaaatgat ggatttactt ttagcaattg tattgattgt gctagctttt
    14461 ctaggaggag ctcttggagg aatgtacttg gttcgtaagc aaatcgaaaa agaatttgct
    14521 gacaacccac gtttgaatgc tgaagcagtt cgtactcttt tgagtgcaaa tggtcaaaaa
    14581 ccaagcgaag ctaaggtaca acaagtttac caccaaatca tccgccaaca aaaggcagcc
    14641 cttgctaaca ataaaaagaa aaaataaata aggaaaagtc tgggatgaaa gttccagact
    14701 tctcactatg ttggttatgg tttagggatg agactttttc cttgcattct attgatattt
    14761 gattatgatt aagagagagg tagttgacat tctatcaagc tgtcggtcag ttcgttcagg
    14821 acaaggaatc ataatgaacg atcaatcaga aaaaagacta gaaagaagac tgtatggata
    14881 atcgaccaat tggttttttg gattcgggtg tcgggggctt gaccgttgtg cgcgagctca
    14941 tgcgccagct tccccatgaa gaaatcgtct atattggaga ttcggcgcgg gcgccctatg
    15001 gcccccgtcc tgctgagcaa attcgtgaat atacttggca gctggtcaac tttctcttga
    15061 ccaaggatgt caaaatgatt gtcattgctt gtaacactgc gactgcggtc gtctgggaag
    15121 aaatcaaggc tcaactagat attcctgtct tgggtgtaat tttgccagga gcttcggcag
    15181 ccatcaagtc cagtcaaggt gggaaaatcg gagtgattgg aacgcccatg acggtacaat
    15241 cagacatata ccgtcagaaa atccatgatc tggatcccga cttacaggtg gagagcttgg
    15301 cctgtcccaa gtttgctccc ttggttgagt caggtgccct gtcaaccagt gttaccaaga
    15361 aggtggtcta tgaaaccctg cgtcccttgg ttggaaaggt ggatagcctg attttgggct
    15421 gtactcatta tccactcctt cgccctatta tccaaaatgt gatggggcca aaggttcagc
    15481 tcatcgatag tggggcagag tgcgtacggg atatctcagt cttactcaat tattttgaaa
    15541 tcaatcgtgg tcgcgatgct ggaccactcc atcaccgttt ttacacaaca gccagtagcc
    15601 aaagttttgc acaaattggt gaagaatggc tggaaaaaga gattcatgtg gagcatgtag
    15661 aattatgaca aataaaattt atgaatataa ggatgaccag gactggtatg ttgggtctta
    15721 tagtattttt ggtggcgtta acagtttgag cgactataag acagattttc ctctgtttga
    15781 attctccaaa atatttggag atgaagagta tggtttcccg ctttcagtta ctgttttacg
    15841 ctatggttct acctaccgtt tgttctcctt tgtggtagat atgcttaatc aagaaatggg
    15901 acgaaacttg gaagttattc aacgtcatgg ggccctgctc ttggttgaaa atgggcaact
    15961 cttgtatgta gaattgccta aagaaggggt caatgttcat gatttctttg agacaagcaa
    16021 ggtcagagaa accttgttga ttgcgactcg taacgaaggc aaaaccaagg aattccgagc
    16081 tatctttgat aagttaggct acgatgtgga aaatcttaat gactaccctg acctgcctga
    16141 agtagcagaa acaggtatga cctttgaaga aaatgcccgc cttaaggcag aaaccatttc
    16201 tcaattaacg ggtaagatgg ttttggcaga tgattctggt ctcaaagtcg atgtccttgg
    16261 tggcttacca ggcgtctggt cagctcgttt cgcaggtgtg ggagcaactg accgtgaaaa
    16321 taatgccaaa ctcttgcacg aattggccat ggtctttgaa ctcaaggacc gctcggctca
    16381 gttccacaca accctagtcg tagccagccc aaataaggaa agtttagttg ttgaagcaga
    16441 ctggtcaggt tatattaact ttgaacctaa gggtgaaaat ggctttggct atgatcccct
    16501 cttccttgta ggagagacag gtgagtcatc agctgaatta accctagaag aaaaaaatag
    16561 tcaatctcac cgtgccttag ccgttaagaa acttttggag gtatttccat catggcaaag
    16621 caaaccatca ttgtaatgag cgattcccat ggcgatagct tgattgtgga agaagtccgt
    16681 gatcgctatg tgggcaaagt cgatgccgtt tttcataacg gcgattctga actacgtccg
    16741 gattctccac tttgggaggg catccgcgtt gttaaaggga acatggactt ctacgccggc
    16801 tacccagaac gtctggtgac tgagcttggt tcgaccaaga ttatccaaac tcatggtcac
    16861 ttgtttgaca tcaatttcaa ctttcaaaag ttggactact gggctcagga ggaagaggcc
    16921 gctatctgcc tctatggtca cttgcatgtg ccaagtgctt ggttggaagg caagatcctc
    16981 tttctaaatc caggttctat cagtcaacca cgaggtacca tcagagaatg tctctatgct
    17041 cgtgtggaga ttgatgatag ttacttcaaa gtggactttt tgacacgaga tcacgaggtg
    17101 tatccaggtt tgtccaagga gtttagccga tgattgccaa ggagtttgag actttcttgt
    17161 tggggcagga ggaaactttt ttgacccctg ctaaaaatct agctgtgttg attgataccc
    17221 acaatgcgga tcatgcgacc ctcttgctca gtcagatgac ctatacccgt gttcccgttg
    17281 tgacagatga aaaacagttt gttgggacga ttggactcag agatattatg gcttatcaga
    17341 tggagcatga cttgagccaa gaaatcatgg cggatacgga tatcgttcat atgacaaaaa
    17401 cggacgtagc ggttgtttcg cctgatttca ccattacgga ggtcttgcac aagctagtag
    17461 atgagtcctt cttaccggtc gtggatgcag agggtatttt ccaagggatt attacgcgca
    17521 agtccatcct caaggccgtt aatgccctct tgcatgactt tagtaaggaa tatgagattc
    17581 gatgccaatg agagacagga tttcagcctt tttagaggaa aagcagggct tgtctgtcaa
    17641 ttccaagcag tcctataagt atgatttgga gcaattttta gacatggtag gtgagcggat
    17701 ttctgagacc agtctcaaga tttaccaagc ccagctagcc aatctaaaaa tcagcgccca
    17761 gaagcgaaag atttcggcct gtaaccaatt tctatacttt ctctatcaaa aaggagaggt
    17821 ggacagcttt taccgcttgg aattagccaa acaagctgaa aagaagacgg aaaagccaga
    17881 gattctatac ctagactctt tttggcagga aagcgaccat ccagagggcc gcttgctagc
    17941 gctcttaatc ctagaaatgg ggctcttgcc cagtgagatt ttagccatca aggttgcgga
    18001 catcaatctg gattttcagg tgctgcgaat cagcaaggct tcccaacaga ggattgtcac
    18061 cattcccacg gccttgcttt cagaattgga acccttgatg gggcagacct atctttttga
    18121 aagaggagag aaaccctatt ctcgtcagtg ggcctttcgt cagttagaat cttttgtcaa
    18181 ggagaaaggt tttccatcct tatcagctca agtcttacgt gaacagttta ttctaagaca
    18241 aatagaaaac aaggtcgatt tgtacgaaat tgcaaaaaaa ttaggattaa aaacagtcct
    18301 gaccttagaa aaatatagat aatggatatt aaattaaaag attttgaagg acccctggac
    18361 ttgctcttgc atctggtttc taagtaccag atggatatct acgatgtgcc cattacggaa
    18421 gtcatcgaac agtatctagc ctatgtctca accctgcagg ccatgcgtct ggaagtgacg
    18481 ggtgagtaca tggtcatggc tagtcagctc atgctgatta agagtcgtaa actccttccg
    18541 aaggtagcag aagtgacaga cttgggggat gacctggagc aggacctcct ctctcaaatc
    18601 gaagaatatc gcaagttcaa gctcttgggt gagcacttgg aagccaagca ccaagaacgg
    18661 gcccagtatt attccaaagc gccgacagag ttgatttacg aagatgcgga gcttgtgcat
    18721 gacaagacga ccattgacct ctttttggct ttttcaaata tcctagccaa gaaaaaagag
    18781 gagtttgcac aaaatcacac gacgatcttg cgggatgagt ataagattga ggacatgatg
    18841 attatcgtga aagagtcctt gattggacga gatcaattgc gcttgcagga tttgttcaag
    18901 gaagcccaga atgtccaaga ggtcatcacc ctctttttgg caaccctaga gttaatcaaa
    18961 acccaggagc tgatcctcgt gcaagaggag agttttggag atatctatct catggaaaag
    19021 aaggaagaaa gtcaagtgcc tcaaagctag acttgataga gaggaaagat gagtacttta
    19081 gcaaaaatag aagcgctctt gtttgtagcg ggtgaagatg ggattcgggt ccgccagtta
    19141 gctgaactcc tctctctgcc accgacaggc atccagcaaa gtttaggaaa attagcccag
    19201 aagtatgaaa aggacccaga ttccagtttg gctttgattg agacaagtgg tgcttataga
    19261 ttggtgacca agcctcaatt tgcagagatt ttgaaggaat actctaaggc gcctatcaac
    19321 cagagcttgt ctcgggctgc ccttgagacc ttgtccatta ttgcctacaa acagccgatt
    19381 acgcggatag aaattgatgc catccgtgga gttaactcga gtggagcctt ggcaaagttg
    19441 caggcttttg acctgataaa ggaagacggg aaaaaggaag tattggggcg ccccaacctc
    19501 tatgtgacta cggattattt cctagattac atggggataa accatttaga agaattacca
    19561 gtgattgatg agcttgagat tcaagcccaa gaaagccaat tatttggtga aaggatagaa
    19621 gaagatgaga atcaataagt atattgccca cgcaggtgtg gccagtagga gaaaagcaga
    19681 agagctgatt aagcaaggct tggtgacggt taacggccaa gtggtgcgtg aactagcaac
    19741 cactatcaag tcaggcgaca aggtcgaagt tgaaggtcaa cctatctaca acgaagaaaa
    19801 ggtctactat ctgcttaaca aaccacgcgg tgtgatttcc agtgtgacag atgataaggg
    19861 tcgcaagacg gttgtcgacc tcttgcccaa tgtcaaagag cgtatttacc ctgtgggtcg
    19921 tttggactgg gatacatcag gtgtcttgat tttgaccaat gatggggact ttacagacga
    19981 gatgattcac cctcgtaatg agattgacaa ggtttatgtc gcgcgtgtta aaggtgtggc
    20041 caataaggac aatctccgcc ccttgacccg tggtcttgag attgatggta agaaaaccaa
    20101 gccagctgtt tatgaaattc tcaaagtgga cccagtcaaa aatcgctctg tggtgcagtt
    20161 gaccatccat gaagggcgta accatcaggt taaaaagatg tttgaagctg ttggtctcca
    20221 agtagataag ttgtctcgga ctcgtttcgg acacctagac ttgacaggac tccgtccagg
    20281 agaatcccgt cgtcttaata aaaaagaaat cagccaacta cacaccatgg ctgtaactaa
    20341 gaaataatga aacgaatttt aatagcgcct gtgcgctttt accaacgttt tatctcacca
    20401 gtctttccac cctcttgtcg ctttgatctg acttgttcca actacatgat tcaggctatt
    20461 gaaaaacatg gttttaaggg agtattgatg ggcttggctc ggattttacg ttgtcatccc
    20521 tggtcgaaaa caggtaagga ccccgttcca gaccactttt cccttaaacg aaatcaagaa
    20581 ggggaatgag gtggggtaaa tagatttcaa aatgataaaa acgcatccta tcaggtttga
    20641 gtgaacttga taggatgcgt tttagaatgt caaaatttta tactcttcga aaatcccttc
    20701 aaaccgcgtc agctttcatc tgcaacctca aaacagtgtt ttgagcaacc tgcggctagt
    20761 ttcctagttt gctctttgat tttcattgag tattaaattg agtttgaagt ggcttatttc
    20821 aaagcttttt gtatgtcttc aatcatgagt tttgttgatt caagtccgcc tccgcttaga
    20881 taccagaggt ctggtgttag ttggataatc ttaccatttt tagcagcagg tgtttcagcg
    20941 ataagggcat tttctaggac accgtcgttg ctagagttgt ccccaccgat ggcaagggta
    21001 cggttgatga caaagaggat gtcagggttg atttctttga cactttcaaa gctgacttct
    21061 tgtccgtggc gtgagtcttc aaattttgta tcagttggtt tgaatttcaa ggtttggtac
    21121 aagaaagaga aacgagattt ggcaccaaag gctgccattt ttccttcatt aaggaggatc
    21181 gcaagggctt ttttgtcaga gctttcattt ttagtagcga cttcttggat gctcttgtct
    21241 agcttggcca attcttcctt ggctttttgt gtactagttt cgccgaaggc acttgctaag
    21301 gattcgatat tagccttggt agaagtccag tagtcatcct tgcttgcttg gaagagaacg
    21361 gttggggcga tttctttgaa tttgtctacg aatttttgtg tacgtggtga agcgataatc
    21421 aaatcaggct caagggcggc gatagcttct aaatcaggtt ctttcataga accaacattt
    21481 ttgacagttc ccactaggtc ttttagataa gtcggaacag tttttgtagg cattccgacg
    21541 atattttttt caaatcctaa agcgcgaata gtatccgcag cgccgaggtc aaaggtcaca
    21601 atcttttcag gaactttgga aagtttgacc tcgtccagtg aacttttaat ggttatctct
    21661 gttggagcag agctactggt ctctgtctga ctagtgcttg agtttgtact acatgcacca
    21721 agtaggagca agaagctggc cactagggca gtgaaataaa gtttaaggga tgttttcata
    21781 atttctcctt tttaaaatgt gataacgatt tagggagtct cttaatctta ttgactaaga
    21841 gactgaaggt tctctaactt gagcttttat gttactagct atagatacag atctttttgt
    21901 cattgatatc agctagcgtg atgggaatct cataaagttg actgagtagg tcagcctgca
    21961 tgatttgatc ggttcttccc ttgctaaaga cctggccgtc cttgaaggcg acaatttcat
    22021 ctgcatactg actggccatg ttgatatcgt ggaggacgat gataatggtc ttgccgagtt
    22081 cctccaccag tcgtcgaaga atctgcatca tgctgacgct ttgcttgata tcgagattgt
    22141 tgagtggttc gtccagcaag ataaagtccg tatcctgggc cagtaccata gcgataaaga
    22201 cgcgctggag ttgcccccct gacaggctat tgatgtagcg gtcttttaag ttggtcagtt
    22261 ccaaatagtt cagagtttct cggatttttt cccagtcttc tgatctaagt cgacctcggc
    22321 tgtagggaaa acgtccaaaa ctgaccagtt cttcaacagt caatttggct tggtaattga
    22381 ttttctgttt taggatggtt agttcttggg ccagttcttg cgaattccag ctctcgattt
    22441 cacgtccttt gatactgaga actccctgat ctttcttggt tagtctgctc atgatggaga
    22501 ggagagtcga ttttccagca ccatttggac caataaaggc tgtcagtttt tgaggactga
    22561 cttcaagcga aatgccttgc aaaatatcct gtttttgaat ggatttgtca atgttttcca
    22621 gtttcactga cgagacctcc tatatagtaa gataaagaat aagaagccac ccacactctc
    22681 aatgatcata ctgatacgaa tttccagtgc aaagactcgt tcaatcaagg cttgccccaa
    22741 ggttaagcta ataaatccaa tcagaatggc cactataaag agtaacttgt gctgatagtc
    22801 tttgacaatc aggtaggtga ggttggccag cataaagccg aagaaggcca taggtcctac
    22861 caaggcagtg gccgttgagg tcaaaagcac gattccccag aggagctctt tctgttcttt
    22921 ttcaacatcg agtcccaata tctgagccgt ttctctttgc aggtgcaaga catctagaac
    22981 gactgctttt cgaaagaaaa agattgtcaa agcgaggatg atcagagaac cgatggctag
    23041 gatggaagtg ttgagatgtt gaaaggaggc aaaaagacta ttttgcagtt tatcgtattc
    23101 gtttggatcc attaggactt gaaggaaggt gctgatattt cgaaagagac ttctgagcgc
    23161 tagacagatc agcaggatga agaccaggtc ttgcttcatc agtgtcttca agtaaccttg
    23221 taaggcgaga aagaagaggg actggacaag aagtaagact aggaattcta agatagggga
    23281 tttgccaagt tgaagaaact tgctttcaaa aaccagtagt agggtttgta gtaggatgta
    23341 gaaggattca attcccaaaa tactaggcgt caggaagcga ttttccgtca gggtttgaaa
    23401 actaatggtc gaaatcccag tcgcgatggc taccaagaga taaacgatga tcttttggga
    23461 acgcaacttc caagcaaagg ctgacaagtg agtgatgggc caaaagtaga gaagacaagc
    23521 tccgatggca agaataatga gaatccagaa gagcttggta tgtttgcttt tagtctgcat
    23581 cttttcgtcc ccctctccag agaagtagga taaagacgag actaccgatg attcctagca
    23641 agagactgac agacaactca tagggcctaa tcagaactcg ggataggata tcgcaagcca
    23701 gaactagatt ggcaccaacc agtgcgacca tgagtttggt ttgacttaga ttatctccat
    23761 agcgcttgcg aacaagattg ggaacgataa ctccgagaaa tggtaggcca cccacggtaa
    23821 tcatggtgac gcttgtcgtt agcgccacca gaaagagggc cagtttttca agtagggagt
    23881 aggaaatccc caaactctcg ctggtttctt tccctagatt catgatggtg aaggtttggg
    23941 ataatttcca aacggttatc aggatgatga ggcctaagaa gagccactca tactgatggg
    24001 tctgaatcat ggagaaggag ccctgggtcc aggcagtcat actctgaacc agattgaaac
    24061 gataggcgat aacttctgtg actgagccga taatcccgct atagatgatc ccaatcagag
    24121 gcaacatcca cctttccttt acagtaaaaa tggtcataaa ggctaggaag aagagggtga
    24181 atacgatgga tgaaacaaaa gcgaagagca tcttttgggt cagactagcc gatggaaaga
    24241 caaaaaggct cagcaccatt cccagtttgg cggcttcagt cgttccaact gtactcggtg
    24301 cagcaaactg attttgggta atagtctgca tgagaaggcc tgccatactc atactagagg
    24361 cagtcaggag aatactgata gttcttggga gacgggactc ttgaaagagg agccaggtct
    24421 gctggtcgaa atcaaatagc tttccccatg aaaaatcact ggtcccaatg ctaatagaga
    24481 gaaagactag gagtagaagt aagccaatta aataatgaga aagtttcata ccccgtcctt
    24541 tcatgtagat ttggtatcga aagatatctg cggatataaa tgtaacatta tttttctaat
    24601 ctgtcaataa aatttctgac aatttaataa atacaacaag gagagagcaa caagactttc
    24661 tcctttgtta tcctattcta aaatgttttt accttaatct gataaaataa tatcttcgag
    24721 ggagtagcta gccgtccaat caagatattg tttagctttt gaagcatctg ctaggacact
    24781 ggctgggtca ctagcacgtc gagcaacaat ctcgtgtggg attttttaat ttagtaattc
    24841 ttcagcagtt ttaaagattt ctttgatagt atagcctttt ttagttccta agttaaagat
    24901 ttgagaagaa ctgtcttctt gaaataggta gttcattcct ttaacatgag cctatgcaag
    24961 gtccaagaca taaatgtaat ctcgaataca tgaaccgtca cgtgtatcgt agtcatctcc
    25021 aaatattttt aagctatcat gttgtcccaa tgcggtcttg ttgatatttg gaatgatgtg
    25081 agttggattt ttcacacgca gaccgtttga agcatccatt tcagccccag caacattaaa
    25141 gtaacggaaa ataacatatt tccagtcgta gcgattggcc atccagtaaa tcattcgttc
    25201 gcccatcagt tttgtctctg cataagggtt gacagggtcg agcagggtat cttcagtcac
    25261 cggcttgtca atacagttat ttccatagag agaagcagtc gaagagaaca tgattttttg
    25321 aatgccaact tcagataaga ctttgagaac ttggttcata ccagcaacgt tggcagtgaa
    25381 gtatttactt ggattttcaa tactttcgct cacaacaatt tcacctgcac aatggaggac
    25441 agcatcaatc tgattttctt ctaagtaagc ctttaaggcg ctagcatcat aaacatccag
    25501 ttctttaaag ctagcacgac tatccacagc cgctcgattt cctgtggaga gattatcgag
    25561 gacatgcacc tgatagccag catttaaaag agctttaacg gtatgggagc caatgtagct
    25621 agtcccacct gtaacaagaa ttgttttggt catgattttt tccttttcta gttttgtact
    25681 atagtaaatt gaaataagat atgaacaact ctattaggaa agtcaaatta atttctagaa
    25741 atgtttcagc agctacagcg tactattcca aactcaacca actatatatt taaagaaaga
    25801 agaaagggaa gtcaactatt ttctgtaaat ttcatgaaaa atccaaacaa ctttgatggt
    25861 gtattgtttt tttgtttgga aataaatatt cgagttaatt ttggaatgtt tactagattt
    25921 tgcactacat gcaagagaat actatccttt tctaagatag agcaggacta agatactaac
    25981 ttagaaatcc accttcccac tatccttctc ctataaaaag tggtaaaatg gatgaaagaa
    26041 agaaggaact gaaatgacaa cattattttc aaaaattaaa gaagtaacag aacttgctgc
    26101 agtctcaggt catgaagcgc ctgtccgtgc ttatcttcgt gaaaagttga caccgcatgt
    26161 ggatgaagtg gtgacagatg gcttgggtgg tatttttggt atcaaacatt cagaagctgt
    26221 ggatgcaccg cgcgtcttgg tcgcttctca tatggacgaa gttggtttta tggtcagcga
    26281 aatcaagcca gatggtacct tccgtgtcgt agaaatcggt ggctggaacc ccatggtggt
    26341 tagcagccaa cgtttcaaac tcttgactcg tgatggtcat gaaattcctg tgatttcagg
    26401 ttctgttcct ccgcatttga ctcgtggaaa ggggggacca accatgccag ccattgccga
    26461 tatcgttttt gatggtggtt ttgcggacaa ggctgaggca gaaagttttg gcatccgtcc
    26521 tggtgatacc atcgtaccag atagttctgc aattttgaca gccaatgaaa aaaatatcat
    26581 ctcaaaagct tgggataacc gctacggtgt cctcatggta agcgagctag ctgaagcttt
    26641 atcaggtcaa aaactcggca atgaactcta tctgggttct aacgtccaag aagaagttgg
    26701 tctgcgtggc gctcatacct ctacaaccaa gtttgaccca gaagtcttcc tcgcagttga
    26761 ttgctcacca gcaggtgatg tctacggtgg tcaaggcaag attggagatg gaaccttgat
    26821 tcgtttctat gatccaggtc acttgcttct cccagggatg aaggatttcc ttttgacaac
    26881 ggctgaagaa gctggtatca agtaccaata ctactgtggt aaaggcggaa cagatgcagg
    26941 tgcagctcat ctgaaaaatg gtggtgtccc atcaacaact atcggtgtct gcgctcgtta
    27001 tatccattct caccaaaccc tctatgcaat ggatgacttc ctagaagcgc aagctttctt
    27061 acaagccttg gtgaagaaat tggatcgttc aacggttgat ttgattaaac attattaaac
    27121 cataagggag gtggtaggga tggtagaacc aaacctagaa agccttataa aagatcttta
    27181 caatcatgct cgacatgatt tgagtgaaga tttagttgct gctctcctag agactactaa
    27241 aaaactgcct actacaaatg agcaattgca ggcagttcgt ctctcaagcc tggtcaatcg
    27301 tgaattgctc ctaaatccca aacatccagc acctgagttg ctcaacttgg ctcgctttgt
    27361 caaaagagaa gaagccaagt acagaggaac tgcgacttct gcgcttatgt atgaggaact
    27421 ttttaaaatg ctttgattcc attgtggatc aaagcatttt ttataagacc tatctcgtga
    27481 gcgttcaaat agtagtaaca aggaatcatg agaagtcagc cgagaccata gtagtgaaga
    27541 aacttctgta atggaagtgg agtgaagtga ggaatcgtca aacggtaggg gataggctgt
    27601 ttgaagggtg ctacgaaaaa gatagggtaa ggtgaaatta tagcggaatg aaataagata
    27661 tgaacaaatt gattagggaa atcaaatgaa tttatagaaa tcttttagca gtcatggcgt
    27721 cgtattctag tttcaaaatg ctataaatac gaacaaattg attgggaaat tcaaatcgct
    27781 ttctatcaat gttttagaag atacagtgcc ctagtccaac ttcaatatac tataaaagaa
    27841 cagatggaag caattatgat ttacttctat ctttaaaata aatgatctga taggaaaaat
    27901 gtgtcccctc ataaaaaata ggatagtacg gatggaaggc ttttgtagac tggaggacga
    27961 aggaatttag aaactctcgt taaaagttag ttgagccgga gtatggtggt gtgaataatt
    28021 atcaaaaatt atttccaaat cgaaatagtt gacagttaga acgatgagtg ttacaattat
    28081 tttattcatt tcgatatcga aataaattgg aggtgttatg aaagatagtc atttgctagc
    28141 ccatcatatt cgtttgttga atgggcggat ttttcaaaag ttactgagtc aagatcctga
    28201 ggctctttat aggggtgaac agggcaagat tttagcggtt ttatggaata gtgaaactgg
    28261 ctgcgcaact gcgacagata tcgcgcttgc gactgggctt gcgaataata cgctgacgac
    28321 tatgataaaa aagctagagg aacaaaagct tgtaattgtt agtccgtgtg gaaaagacaa
    28381 gcgtaagaag tatttagttt taacggagtt aggcaagttc cagaaagaag tggggcatcg
    28441 tgtcagtcag aaattggata ctatctttta caaaggattt tcagaggaag aaattcacca
    28501 atttgaaggt tttcaagaaa gaattttggc aaatctgaaa gagaaggaaa atgaggttta
    28561 gaatatgtca caacaagtga aaaatgctca caacctatac atccatgcca ttcaagacgg
    28621 acgagttgct gaggctcaag cccagtctgt aggggatacc tatatccaac attcgacagg
    28681 tgttcctgac gggaaagaag gatttgcagc cttctttgca gatttctttg agcgtcatcc
    28741 agagcgtcag attaagattg tccgcaccat tgaggatggc aatctggtct ttgttcatgt
    28801 tcatcaatat ctgaatggtg gagaagctca atgggtgacg acggatactt tccgtgcgga
    28861 tgagaatggt cgtattgttg agcattggga tgtcattgac tactatcgaa cacctgaaaa
    28921 tgatcaacta gatcaaattt ttggagattt tgaaatcaag gatttggaca agaaagcaga
    28981 aaataaaaag ctggttcgcc gtttcttgac agaaactttc caaaatgggg agctagagca
    29041 gtggagtgat tatgtggcag acgatttgat tcagcataat catgagattg gacaaggaag
    29101 tgctgcttat aaaaactatg tggctgaata tagtgtcact tttgactttg ttttccaact
    29161 cttaggacaa ggaaactatg tggttagcta tggtcagact cagattgatg gcgttgctta
    29221 tgcccagtac gatatcttcc gtttagagaa cgggaaaatt gtggagcatt gggataataa
    29281 ggaagtcata cctaaggtag aagacttgac taatcgaggg aagttttaaa ttgaggacaa
    29341 agaatgattg aatacaaaaa tgtagcactg cgctacacag aaaaagatgt cttgagagat
    29401 gtcaatttac agattgagaa tggggagttc atggttttag ttgggccttc tgggtcaggt
    29461 aagacgacca tgattaagat gattaaccgt ctcttggaac caactgatgg aaatatttat
    29521 atggatggca agcgcatcaa agactatgat gagcgtgaac ttcgtcttac tactggatat
    29581 gttttacagg ctattgctct ctttcccaat ctaacagttg aggaaaatat tgctctcatt
    29641 cctgaaatga agggttggac taaggaagaa attgctcaaa aaacagaaga gcttttggca
    29701 aaagttggat taccagtagc tgagtatggg catcgacttc caagtgagct atctggtgga
    29761 gaacagcaac gggtcggtat tgtccgagct atgattggtc agcccaagat tctcctcatg
    29821 gatgaaccct tttcagcctt ggatgctatt tctcgcaagc agttgcaggt tctgacaaaa
    29881 gaattacata aagagtttgg gatgacaacg atttttgtga ctcatgatac ggatgaagcc
    29941 ttaaagttgg cagatcgtat tgctgttttg caggatggag agattcgtca ggtgtcgaat
    30001 cctgagacta ttttaaaagc tcctgcaaca gactttgttg cagacttgtt tggaggtagt
    30061 attcatgact aatttaattg caacttttca ggatcgtttt ggtgattggt tgacagccct
    30121 atctcaacat ttgcagttgt cacttttgac cttgttacta gctattttgc ttgcgattcc
    30181 cttggctgtt tatcttcgct atcatgagaa gttggcggat tgggtcttac agattgcagg
    30241 gattttccag accatcccgt ctctggcctt gttgggactc tttattccct tgatgggaat
    30301 tgggacttta ccggcattga cagctctagt gatttatgcg atttttccga ttttacaaaa
    30361 tactattact gggctgaagg gaattgatcc gaacctgcaa gaggctggga ttgcctttgg
    30421 gatgaccaga tgggagcgtc tcaagaagtt tgaaattcca cttgccatgc ctgttatcat
    30481 gtctggaatt cggacggcag cggtcttgat tatcggtacg gctaccttgg cgaccttgat
    30541 tggggcaggg ggactgggtt cctttatcct gttgggaatt gaccgtaata atgccagttt
    30601 gattttgatt ggggcccttt cttctgcagt gctagctatt gcctttaact tcctactaaa
    30661 agtaatggaa aaagcgaaat tgcggacgat tttctctggt tttgccttga tggctttatt
    30721 actgggtctg tcttatagtc cagccctttt ggctcaaaaa gagaaggaaa acttgattat
    30781 tgctgggaaa ataggcccag aaccagaaat tttggctaat atgtataaat tgctgattga
    30841 agaaaatacc agcatgactg cgactgttaa accgaatttt gggacgacaa gtttccttta
    30901 tgaagctctg aaaaaaggtg atattgacat ttatcctgaa tttaccggta cggtgactga
    30961 aagtttgctt caaccatcac ccaaggtgag tcatgagcca gagcaggttt atcaagtggc
    31021 gcgtgatggc attgccaagc aggatcatct agcctatctc aaacctatgt cttatcaaaa
    31081 tacctatgct gtagctgttc cgaaaaagat tgctcaagaa tatggcttga agaccatttc
    31141 ggatttgaaa aaagtggaag ggcagctgaa ggcaggcttt acgcttgagt ttaacgaccg
    31201 tgaagatgga aataagggct tgcaatcaat gtatggtctc aatctcaatg tggcgactat
    31261 gcagccagct cttcgttatc aggcaattca gtcaggggat attcaaatca cggatgccta
    31321 ttcgactgat gcggaattgg agcgttatga tttacaggtc ttggaagatg acaagcaact
    31381 cttcccacct tatcaagggg ctccactcat gaaagaagct cttctcaaga aacacccaga
    31441 gttggaaaga gttcttaata cattggctgg taagattaca gaaagccaga tgagccagct
    31501 caactaccaa gtcggtgttg aaggcaagtc agcaaagcaa gtagccaagg agtttctcca
    31561 agaacaaggt ttgttgaaga aatgatggga cagagacttt aaacatactg aactcaactt
    31621 ccttaaacga ctagataaaa gaatgctcag acaatttcgt ctgggctttt ttgatgtgag
    31681 aatgactgaa tgtcacttaa aatggaaaga aagagaaaat tttcatgatt ttctaaaatt
    31741 ttactgtaaa gacagttgac tattcataaa ataggtgtat aatattttgt gaactactta
    31801 acaaataaga ggtttcagct catgtcgact aaggttagaa atcttgtgta taggcagttc
    31861 attatctaat agagcgttgc gtccgaaagt ctatccagac acggctcttt aaaaacaaaa
    31921 ggagaaatga tgcatactta tttgcaaaag aaaattgaaa atatcaaaac aaccctaggt
    31981 gaaatgtcag gtggttaccg tcgtatggtt gcggctatgg ctgatttagg attttcagga
    32041 actatgaagg ctatctggga tgacctcttt gcccatcgta gttttgccca gtggatttat
    32101 ttgctggttt taggaagttt tcctctctgg ctggagttgg tttacgaaca tcgtattgtt
    32161 gactggattg ggatgatttg tagcttgaca gggattatct gtgtaatctt tgtatcggaa
    32221 ggtcgagcaa gtaattatct ttttggcttg attaactctg ttatttacct tattttggcc
    32281 ctacagaaag gcttttatgg tgaggtgctg acgacacttt acttcacagt catgcagcca
    32341 attggacttc tagtttggat ttatcaggca cagtttaaga aggaaaagca ggagtttgtc
    32401 gcgcgtaaac tggacggcaa gggctggaca aagtatcttt ccattagtgt gctttggtgg
    32461 ttggcctttg gcttcattta tcagtctatt ggtgccaatc gtccctatcg tgattcaatc
    32521 acagatgcaa ccaatggggt agggcaaatc ctcatgacag ctgtttaccg tgaacagtgg
    32581 atattctggg cggctaccaa tgttttttca atctatctct ggtggggaga aagcctgcaa
    32641 attcaaggga aatatctaat ttatctcatt aacagtctag ttggttggta tcaatggagc
    32701 aaggcagcta agcagaatac tgatttactt aactaggaaa agatgtttga aagtgctgtt
    32761 ttgagatttc gattaaaaca gatatagttg ataatcaagg atttatagta tgaaaaagag
    32821 gatcggcggg tcctcttttg ttgttgaaaa gataaaaaac tcagtaacct agaaataaga
    32881 caactgagct ttactctata ttcaattttt aggaatgaga aggtctagat aaaattggac
    32941 aacttcctgg tctgtgaaat cttgaccttt tttgagccac caggtcaatg tctcgataaa
    33001 gttggacatg accaagtgtt ggaggtaaga agtaggcaga ttagggtggg cttcttttaa
    33061 attatcagct agcacggaat agacgtggtg ctcaagctct ctctggagtt gacggaggaa
    33121 gtagtcattt ttggaaaata gcagactggt gatatggtct tggtttttat gaaaatggag
    33181 aaagaggtgg gcgaggtagt cctcggttga aatggcttgc tctctttcaa aaagatgatg
    33241 gaagaggtag cggcagagct gatccagaag aagctcttta ctctcatagt gacagtaaaa
    33301 ggtggatcgt cccacatctg cgagatcaat gatatcctga acagtagtgg tctcgtagtc
    33361 cttagcattc aaaagttgta taaaagcttg atagatggct tttttggttt tgctgatacg
    33421 gcggtcaatg ttagtcatat ggacacttga ggcaaattgt tcagaactga ataaagctga
    33481 cgttttgctt ctatcctttc tttgagtttt agtggataat gataatgaac aaggtgttca
    33541 taaatctatt ataacaaagg aatgagaaat atgaaggcaa aatatgctgt ttgggtggct
    33601 tttttcttaa atttgactta tgccattgtt gagtttattg caggtggagt atttggttct
    33661 agcgctgttc ttgctgactc tgtgcatgac ttgggagatg cgattgcaat tggaatatca
    33721 gcttttctag aaacaatctc caatcgtgaa gaagacaatc agtacacctt gggctataag
    33781 cggtttagcc tgctaggagc cttggtaaca gctgtgattc tcgtaacggg ctctgttcta
    33841 gtcattttgg aaaatgtcac gaagattttg catccgcaac cagtcaatga tgaggggatt
    33901 ctctggttag gaattattgc gattactatc aatctgttag cgagtctggt ggttggtaag
    33961 ggaaagacaa agaatgagtc tattctgagt ttgcattttc tggaagatac gctagggtgg
    34021 gtagctgtta tcctgatggc gattgttctt cgatttacgg actggtatat cctagatcct
    34081 cttttgtccc ttgtcatttc tttctttatt ctttcaaaag cccttccacg tttttggtct
    34141 acactcaaga ttttcttgga tgctgtgcca gaaggtcttg atatcaagca agtaaagagt
    34201 ggcctggagc gattggacaa tgtggccagc cttaatcagc ttaatctctg gactatggat
    34261 gctttggaaa aaaatgccat tgtacatgtt tgtctaaaag aaatggaaca tatggaaact
    34321 tgtaaagagt ctattcgaat tttcctaaaa gattgtggtt ttcaaaatat taccattgaa
    34381 attgatgctg acctagaaac tcaccaaacc cataagcgaa aggtgtgtga cttggaacgg
    34441 agttatgagc atcaacatta gaaaaaagtg aaaaatactt gggtactatc tt
//
LOCUS       DAVLSL010000005        31581 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLSL010000005 DAVLSL010000000
VERSION     DAVLSL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.005"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..2100)
                     /locus_tag="VXN30_000180"
     CDS             complement(<1..2100)
                     /locus_tag="VXN30_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEV1356862.1"
                     /translation="MAETFEGLYVKFGANTVEFDRSVKGINNALSSLKKDFNNINRQL
                     KMDPDNVDLLNRKLLNLQEQARVGAMKIAELKKQQKELGESEVGSAQWNKLQLEISKV
                     ESQMKVVDQTMNSTKKHIEDVGNPKSILNLNKEINNVAKELDIVNQKLELDPKNVELS
                     EEKMKLLGKQSSLAKDKVQELKRKQEELGKEKIGTEEWRQLQNEIGQAEVEVLKIDKA
                     MGNLGDSSRSATGNIKEATGYLKADVMMNIAEKAGQLGQKMVDAGKKTVGAWSEIDEA
                     MDTVTTKTGLTGEALLELQEIAKGIATSLPSATFQESADAVGELNTQFGLTGDTLQSA
                     AEYLLKYSKITGEDISNSAINAKKAIDAYGLSNEDLARVLDSVTKVGQDTGQSYDSIF
                     QKAIDGAPQIKMLGLSFKEGATLIGRFEKSGIDSSAALASLSKATVNYAKDGKTLTEG
                     LNETVNAIQNATSKTEAIRIASEVFGNRAAPKMVDAIQRGAFSFNDLAEAAQNSSGTV
                     ATTFAETKDPFDDLTTYSNKAKEGLAEIGGTLLETVIPALEPLMGMLESAVNWFTSLN
                     ETDQQTIVILGLVTTAVMLLLGAIAPLVIAIGAIGAPVGIVVAAIVAAIAAITLIIQA
                     IMNWGTISEWLQSTWDACAAWLSELWTNIVTTATTAWSSFTAWLSEIWSSVVSTGQSL
                     WSSFTSTLSNIFSSLISG"
     gene            complement(2287..2763)
                     /locus_tag="VXN30_000181"
     CDS             complement(2287..2763)
                     /locus_tag="VXN30_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356863.1"
                     /translation="MIKKELSFTAFDSYGEEREYTGTVRFLYSLPAIKMYEQRTGRNF
                     FDDNQKALTAYTQLALATGVNGRLSALTDEEKVKLMPLLMEPDFMNFLTEVIPCLYGE
                     VENGRLVQNELTAETASLAPWFGDLIDIGFFSDLFYEFNRSRAKVPQDRKKPQQKS"
     gene            complement(2765..3433)
                     /locus_tag="VXN30_000182"
     CDS             complement(2765..3433)
                     /locus_tag="VXN30_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1356864.1"
                     /translation="MTYKYDTREVTHGNTTGFFAKISKTESGTLDLKKTYPFTGLRKT
                     SFETSQESNAYYADNVEHVRLQGKKSTEGSITTYQIPKQFMIDHLGKKLTTSTPPALI
                     DTGVNANFIWGYAETVTDEFGSEVEEFHIWTNVKASAPKGSTTTDESSATPKEIEIPC
                     TASPNNFILDSDKKPVSEIVWRDTDKGVVRAKFDKLFASSTPMKLIDFINEALGTTAI
                     VPGG"
     gene            complement(3447..3791)
                     /locus_tag="VXN30_000183"
     CDS             complement(3447..3791)
                     /locus_tag="VXN30_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1356865.1"
                     /translation="MGSGSVNAKTLKIDIQNQVLEIIEKSGKSTAGDIRDGSPRRNGV
                     YEKGWTHETIEDIAVVYNNGKEKSLAHLLENGHATKNGGFVAPQEHIRPAYLKNKEIF
                     LNNMKSIKIRPN"
     gene            complement(3778..4125)
                     /locus_tag="VXN30_000184"
     CDS             complement(3778..4125)
                     /locus_tag="VXN30_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356866.1"
                     /translation="MAKKFFTRQEIQEILEKNTLKSKVFYMEREEKSSPDNVILYYRL
                     TPGSSITADDTVHMRKVTIQISHYHKKKLDSIEELMLSNFMCEPSQLNLKQPDTDYLL
                     TTYRIEVFTSGKW"
     gene            complement(4125..4430)
                     /locus_tag="VXN30_000185"
     CDS             complement(4125..4430)
                     /locus_tag="VXN30_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEV1356867.1"
                     /translation="MKSSRVSIILCYDERTEVEKGVFEKQVVEKKVKAEKEKIYQRRL
                     DKALADGQVLTARFRIRSNYVTDSLDYVKYKGKEYKVNVGTESDDDHYTIIELGELK"
     gene            complement(4427..4762)
                     /locus_tag="VXN30_000186"
     CDS             complement(4427..4762)
                     /locus_tag="VXN30_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356868.1"
                     /translation="MTILNQIKEMVEVDVEEEIFDTQFLSYINSGISYLTRNNIPINR
                     IDKESELTEWNEIEEDDKETILDWLHLRCVQRFDKSLMTGNSTTRSWIDEELTNILYQ
                     LKAIYGVKS"
     gene            complement(4788..6041)
                     /locus_tag="VXN30_000187"
     CDS             complement(4788..6041)
                     /locus_tag="VXN30_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV1356869.1"
                     /translation="MKTLVELMEERQKHADELSEIKFKKASIEEKLESATIGEEELAQ
                     LKSDAEGLVSKADELKNTISKLDVEIEEKQDNLNKAAKSIKEVQKGKIQMEYLKTKEA
                     ALDFARILMDNEGSSNSARKAWEANLVEKGVTDVNKILPEPVLIAIQNAFNDYDGILN
                     HVTKDPRYAVRVALQTQQAKAKGHQNGKTKKDESFVFIDYTINSAAVYIKYSFEYADL
                     KKDTTGAYFNYVMNELAQGFIRAVERAVVIGDGKNSDDDDKITEIKSIAEETLAQLFD
                     TQEISVDGEFDSTVLENLVKGIDKLAANTTPILVTSKTIARKLKMVKDGEKRYIDPQP
                     FAPISQTGNVIAGYQVYVYDWMEDATNPIIAFADKAYKMIGDDVSADRFEDYDVTMNR
                     RHIELASVLGGRLGQYKSAVKFTKG"
     gene            complement(6056..6598)
                     /locus_tag="VXN30_000188"
     CDS             complement(6056..6598)
                     /locus_tag="VXN30_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEV1356870.1"
                     /translation="MEKLKTFVVKSVEEESADFHFEAYASTYGNTDRDGDVMAKGCFD
                     NTLKTKAVVPMCLNHDRNRVIGKHELSVDEKGLRTRSTFNLSDPEAKKTYDLMKMGAL
                     DSLSIGFFINDYEPVDAKQPYGGWIFKEVEIFEISVVTVPANPQATIDNIKGFDMSVV
                     DKRIAQANMKQDIMSKLATI"
     gene            complement(6645..7787)
                     /locus_tag="VXN30_000189"
     CDS             complement(6645..7787)
                     /locus_tag="VXN30_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1356871.1"
                     /translation="MNKYINNLREVFARIFRPSNRKSTRTYLQRNLNYWRRNSIYLDN
                     IYNKISTDTAQVRFKHVRITRNPTGVDKMEWFENSDLANVLSFSPNPLEIPVVFWANV
                     TRAMLKDGVAVVVPRWENGRLIEIWFAKKTISWTAERVEIMIDDVEIELPLSDVWVFE
                     NPKLNVTSQLNQITELIDINLDALTEKLGRGNSKLRGFLKLPTKAADEHLKKQAKSRV
                     DSMMELAEIGGIAYLEQGEEFMELNKDYSTASKEEMEFLKSQLYHAHGINEKLFTCDY
                     TEEQYRAYYSSVMKLYQRVFSEEINRKYFTKTARTQGNKLLVFFDMADMISFKDLVEG
                     GFKSKYAGLMNSNEFRETYLGLPGYEGGEVFETNLNAVRIEPSESN"
     gene            complement(7796..9508)
                     /locus_tag="VXN30_000190"
     CDS             complement(7796..9508)
                     /locus_tag="VXN30_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEV1356872.1"
                     /translation="MVEMRYFDKYAQLVYSGKIRVCELTMKSIKRVERYKEQYIFKQE
                     EVDKRIEFIEEECSNTKGLAGKLHLALPQKVWLETTWGFYHTVEVTKTDPDTLEEYKD
                     FEERRLIHEVPIIVPRGTGKTTLGSAIGEVGQIIDGEWGADIQLLAYSREQAGYLFNA
                     SRAMLSNEESLLHYMREADILRSTKQGILYETTNSLMSIKTSDYESLDGTNAHYNIFD
                     EVHTYDDDFIKVVNDGSSRKRKNWITWYISTNGTKRDKLFDKYYNIWVDILDEKIVNH
                     SVMPWIYQLDDVSEIHNPDMWQKAMPLLGITTEKETIAKDIEMSKNDPAQQAELMAKT
                     FNLPVNNYLAYFSNEECKGWLDKFDKSLFVGNEERSARCVLGVDLSDVNDICSVSFMV
                     VRGEERQYLNKKFMPRHTIEGLPKELRDKYAEWELSGQLHVHELDYNDQAYIFEELRQ
                     FMSENRILPVAVGYDRWNAKELIRLINDYYGDICHDIPQTVKSLSNPLKVYKEKAKMG
                     KIIFDDPVATWNHANVRVKIDANNNVFPNKEKAKEKIDVFASQLDAFICYENFKEDLS
                     YYFD"
     gene            complement(9501..9986)
                     /locus_tag="VXN30_000191"
     CDS             complement(9501..9986)
                     /locus_tag="VXN30_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356873.1"
                     /translation="MILKINEVLEKLGISRATLTRYRKKLGIFEETRSNITKSQFKEL
                     EKLANQRQKYTREERVELSRKTFKLIPKEKMLEINDNDSVGLKNLKTQYNHNQKVIEN
                     FQLEINKVINDGELPDKYLLDGMEKYQKLNMQIMSTIEKQSPQGDSLKEMIQEKLARY
                     G"
     gene            complement(10123..10440)
                     /locus_tag="VXN30_000192"
     CDS             complement(10123..10440)
                     /locus_tag="VXN30_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1356874.1"
                     /translation="MTFKNPKHSDWFKSWQIKFYNSKPWRTLRNRIRTTKRMRCDVCG
                     RLIHGKSIVDHIIEIDETNYQDESITLNEDNLQLLCLECHNTKTFQSKINLNLENRNI
                     NLF"
     gene            complement(10870..11412)
                     /locus_tag="VXN30_000193"
     CDS             complement(10870..11412)
                     /locus_tag="VXN30_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1356875.1"
                     /translation="MNIVEPLRDKDDIQAMKDYLSSWNEKYYMLFLLGINTGFRVGDI
                     LKLKVKDVQGWHIKVREQKTGKYKSIKMTRPLKNELREFVKDKELHEYLFQSRVGKNK
                     ALSYKTVYWFLKRAAEDLGIDNVGTHTMRKTFGYHYYKKYKNVADLMSLFNHSSPAVT
                     LIYICVRQDELDTKMSNFSL"
     gene            complement(11521..11985)
                     /locus_tag="VXN30_000194"
     CDS             complement(11521..11985)
                     /locus_tag="VXN30_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356876.1"
                     /translation="MGKKNLITARRDYLEFELNDKYLQIDKLIGQRRHELERLYEVKH
                     LTVPGIDDTGASGSGTFVNRSENLAVAYASDPMVLRLENFQTAISKLLDVLEPDDKKI
                     FHLKWGEHTRYDWIQVWHKMENGDTGYLYRHSKQIYRRREIILDTLAKLLFM"
     gene            complement(12102..12389)
                     /locus_tag="VXN30_000195"
     CDS             complement(12102..12389)
                     /locus_tag="VXN30_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356877.1"
                     /translation="MIQTLEEGMKNQSKRIKIPMEIRPFDVGYRIVNKHGQALALKNG
                     ASIFALPSLAEKAIKKEFSKNDPDFDIEKHFVEEVAIVNLSKFHSYFEDRV"
     gene            complement(12422..12718)
                     /locus_tag="VXN30_000196"
                     /pseudo
     CDS             complement(12422..12718)
                     /locus_tag="VXN30_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
     gene            complement(12720..13037)
                     /locus_tag="VXN30_000197"
     CDS             complement(12720..13037)
                     /locus_tag="VXN30_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356878.1"
                     /translation="MMEELKKKVNEVYNWTVEDGKPQPPQQDLPQAVKDRADYFREMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     GGN"
     gene            complement(13009..13341)
                     /locus_tag="VXN30_000198"
     CDS             complement(13009..13341)
                     /locus_tag="VXN30_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356879.1"
                     /translation="MKYKVTEYHSDFQEEQTGTCELCFGTAWVENGSITVEDENGTET
                     EIYLTVWDWGDYDTIYIDNVVNFSAWLQEREVDPIIEETEPWSWLHELVEKYNEELED
                     DGRVKEKS"
     gene            complement(13331..13522)
                     /locus_tag="VXN30_000199"
     CDS             complement(13331..13522)
                     /locus_tag="VXN30_000199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356880.1"
                     /translation="MNYDKRTVIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LKKKNKELRKKVEELEDEI"
     gene            complement(13512..13676)
                     /locus_tag="VXN30_000200"
     CDS             complement(13512..13676)
                     /locus_tag="VXN30_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356881.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEL"
     gene            complement(13813..14583)
                     /locus_tag="VXN30_000201"
     CDS             complement(13813..14583)
                     /locus_tag="VXN30_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1356882.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQERVNELANAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LVFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLTS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            complement(14723..15502)
                     /locus_tag="VXN30_000202"
     CDS             complement(14723..15502)
                     /locus_tag="VXN30_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1356883.1"
                     /translation="MALKNKRYFWIQLAQDFFKSKEMKLLRKIAGGDTHTIIYLKMML
                     ISLEDGGHIYYDGLADNLAEEIALVIDENVEDIKITLIFLESKGLLTRNSDRDYFLEQ
                     VPEMVGSETASTRRSRKHRELQKLHCNTIATTCNGDIDIDKEIEKDKNNKVMISSSLS
                     ENLKNSGIHLTDKSHQQLLDYVGLDGMSFDMLNRAVEKTSGSHKPSFNYLIAILESWK
                     KKGFTSIEQVDEDDRKYKEGKKSRQQNDKTSEQEVRDEWGF"
     gene            complement(15493..15792)
                     /locus_tag="VXN30_000203"
     CDS             complement(15493..15792)
                     /locus_tag="VXN30_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356884.1"
                     /translation="MEELIESLDNLIMIVKELEGRESTSRHFITIWENDYKNLLLVKE
                     YLTDYEKLAKDYRYVTLKNKLLKIEKMELEGRHIYEDMRMKYRANCRKWGVRLWR"
     gene            complement(15808..16128)
                     /locus_tag="VXN30_000204"
     CDS             complement(15808..16128)
                     /locus_tag="VXN30_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356885.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQVRNHAYERGREDKWQEIRKNIRREFKGFTYDNEPPVGLRPEPLALPEPKQSA
                     IRFL"
     gene            complement(16214..16471)
                     /locus_tag="VXN30_000205"
     CDS             complement(16214..16471)
                     /locus_tag="VXN30_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1356886.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCE
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            complement(16484..17200)
                     /locus_tag="VXN30_000206"
     CDS             complement(16484..17200)
                     /locus_tag="VXN30_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEV1356887.1"
                     /translation="MNEIFNFHGQEVRTLTIDDEPWFVGKDVADILGYANSRKAIFDH
                     VDDDDKTDGVTIRDAMGRNQNPIIINESGLYSLILSSKLLQAREFKRWVTSEVLPAIR
                     KQGGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAQSLLKKRK
                     ARVVACLGGIDSPAYADKIFAQSVFRQAEIDFKDHFNISRYDLLPKKHADAALAYWMT
                     WEPSTNTKMKIMKLNSFDEG"
     gene            complement(17343..17546)
                     /locus_tag="VXN30_000207"
     CDS             complement(17343..17546)
                     /locus_tag="VXN30_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1356888.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVFVTSSVISELLKYG
                     KGSESVKEKVADVLGIENPWRNH"
     gene            complement(17558..17704)
                     /locus_tag="VXN30_000208"
     CDS             complement(17558..17704)
                     /locus_tag="VXN30_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356889.1"
                     /translation="MEDKIIELADYFISENTTYREAKIACEKLFKQASHEIELRALES
                     ETKK"
     gene            17823..18044
                     /locus_tag="VXN30_000209"
     CDS             17823..18044
                     /locus_tag="VXN30_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356890.1"
                     /translation="MSKLTKEDVLQVSQEIINDAIPVIKDMLDEVFKEYPIDMEIRKA
                     ILNSVLVAHKLSTETTVSLLTELVNAQEN"
     gene            18422..18784
                     /locus_tag="VXN30_000210"
     CDS             18422..18784
                     /locus_tag="VXN30_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1356891.1"
                     /translation="MFFTFEKIKELADKQGISLNKLEEKLGFSRNTIYNMKKSTPNVE
                     RVSMIADYFNVSTDYLLGRTDNPTIAGSHDYKWEGKTLNVEEMASNVMMFGGRELTDE
                     KKKIIQSIIEGYLKEAGD"
     gene            18797..19180
                     /locus_tag="VXN30_000211"
     CDS             18797..19180
                     /locus_tag="VXN30_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1356892.1"
                     /translation="MTEKEIISHFQIRIIDFDGDLMPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDAIESLDDPTEFDYLK
                     FMSYYNLKTMTNEVMVKEEYFALME"
     gene            19193..20116
                     /locus_tag="VXN30_000212"
     CDS             19193..20116
                     /locus_tag="VXN30_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEV1356893.1"
                     /translation="MSYSYVALDVETANDFRGSVCSIGLVKFKDGNIVDAFYTLINPE
                     EEFDDFNIFIHGITPEDVLDSPTFPEVRKAIVDFIGSDIVVAHFAQFDMGALKDVYQK
                     YELDFDNIEYICSYRLAKVALPGQLNYKLKRLAKNLNIELDHHNALSDARASGLILEY
                     LLSTNSFSDLNAFLKEYSYNKTGLLGQYGFKRKKSYQYKENLIYQPTEEEKAAMNPDH
                     YFYGLYFCFTGKLERMTRKEANKATALVGGIPEKGVTKHTNILVVGEQDWRVVGTDGL
                     SSKMKKAQTLLEKGQDIEIMTENDFIRLLEE"
     gene            20305..21453
                     /locus_tag="VXN30_000213"
     CDS             20305..21453
                     /locus_tag="VXN30_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1356894.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSILLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQGIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            21786..21935
                     /locus_tag="VXN30_000214"
     CDS             21786..21935
                     /locus_tag="VXN30_000214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356895.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RKRKR"
     gene            22077..22256
                     /locus_tag="VXN30_000215"
     CDS             22077..22256
                     /locus_tag="VXN30_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356896.1"
                     /translation="MRKVIEELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(22526..23482)
                     /locus_tag="VXN30_000216"
     CDS             complement(22526..23482)
                     /locus_tag="VXN30_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1356897.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKNTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(23486..23818)
                     /locus_tag="VXN30_000217"
     CDS             complement(23486..23818)
                     /locus_tag="VXN30_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1356898.1"
                     /translation="MQQITEIIIAFATSFLTVAVGGIVKAVKDYLLRKGGEKAVIIAE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            complement(23822..24238)
                     /locus_tag="VXN30_000218"
     CDS             complement(23822..24238)
                     /locus_tag="VXN30_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV1356899.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFVFLHSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            complement(24248..24598)
                     /locus_tag="VXN30_000219"
     CDS             complement(24248..24598)
                     /locus_tag="VXN30_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356900.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            complement(24601..24804)
                     /locus_tag="VXN30_000220"
     CDS             complement(24601..24804)
                     /locus_tag="VXN30_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356901.1"
                     /translation="MNTEQLNQALQMTISEMSTTSTNSMITSNILSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(24899..30865)
                     /locus_tag="VXN30_000221"
                     /pseudo
     CDS             complement(24899..30865)
                     /locus_tag="VXN30_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
BASE COUNT         8708 a         6945 c         5386 g        10542 t
ORIGIN      
        1 tcctgaaatc aaacttgaga aaatattgga caaggtgcta gtaaagctag accacaaaga
       61 ctgtcccgtt gagactactg aagaccaaat ttcagaaagc caagcagtga aacttgacca
      121 cgctgtagta gcagtcgtaa caatattagt ccacaattca gaaagccaag cggcgcaagc
      181 atcccacgtc gactgaagcc attcagatat ggtcccccag ttcatgatgg cctgaatgat
      241 gagtgtaata gcggcaatag cagcaactat tgccgctacg acaattccga caggcgcacc
      301 tattgcacct atagcaatga ctagcggtgc tattgcacca agcagtagca ttacagcagt
      361 tgtaacgagg ccaagaatca cgatagtctg ttgatcagtt tcatttaagc tggtaaacca
      421 attaacagca gattcaagca tgcccatcaa aggttctaaa gctggtataa cagtctcaag
      481 tagtgtacca cctatctcgg caagcccttc ttttgctttg ttggaatagg ttgttaggtc
      541 atcaaatgga tcttttgtct cagcaaatgt tgttgctaca gttcctgatg agttttgtgc
      601 tgcttcagct aaatcattaa aactaaatgc cccacgttgg atagcatcta ccattttagg
      661 agcggctcta tttccgaaaa cttcagaagc aattcttatt gcttctgtct tactagtagc
      721 attctgaatt gcattgacag tctcgttcaa cccctcagtc aatgtctttc cgtctttagc
      781 atagtttact gtagcctttg aaagtgaagc taaagctgca gaagagtcaa tcccactttt
      841 ttcaaatcta ccaattaatg tcgccccctc tttaaaagat aatcctagca tcttaatctg
      901 tggagctcca tcaattgctt tttgaaagat ggagtcataa gattgaccag tatcctggcc
      961 gacctttgtt actgagtcca atactctcgc tagatcctca ttagataaac cgtaagcatc
     1021 aattgctttc ttggcattta ttgcggaatt tgaaatatct tctccagtta ttttcgaata
     1081 tttcaatagg tactctgctg cagattgcaa agtatcacca gtaagtccaa attgtgtatt
     1141 taactcacca actgcgtcag cagattcttg aaatgtagcc gatggtaagg atgtagcgat
     1201 tccttttgca atttcctgaa gttctaacaa ggcttcgcca gtaagtccag tcttcgtcgt
     1261 aacagtatcc atcgcttcgt cgatttcaga ccatgcacct actgtttttt taccagcatc
     1321 aaccattttt tgacctagtt gtcctgcctt ttcagcaatg ttcatcatta catcggcttt
     1381 taagtatcct gtagcttcct tgatgtttcc tgttgctgaa cggctcgaat cccctagatt
     1441 ccccatggct ttatctatct ttaacacctc gacttctgct tgcccaattt cattttgaag
     1501 ttgtcgccat tcctctgttc cgattttttc ctttcctaat tcctcttgtt tccgtttcaa
     1561 ctcctggacc ttatccttgg ctaatgaaga ttgtttacct aataacttca ttttttcttc
     1621 ggacaactct acatttttag gatctaattc aagcttctgg ttgacgatat caagttcttt
     1681 tgcaacattg ttgatttctt tgttgagatt taaaatagac tttggatttc ctacatcttc
     1741 gatatgtttt ttggttgaat tcattgtctg gtcaacaacc ttcatctgtg attcaacttt
     1801 agaaatttca agttgaagct tattccactg tgctgaccca acttcagatt ctcccagttc
     1861 cttttgttgc tttttgagtt cagcaatttt catagcacca acacgagctt gttcttgtaa
     1921 gttgagcaac ttacgattca gcaagtcgac attgtctgga tccatcttca attgtctatt
     1981 gatgttgttg aaatcttttt tcagactaga taaagcatta ttgatacctt ttacagatct
     2041 gtcaaattca acagtattag caccaaattt gacatataag ccttcaaatg tttcagccat
     2101 agatttcctc ctttcagttt tagtcagaca ttacatttag taattctgcg tttgataaag
     2161 ttttcttctc attttcattg atactcatct gatgtagtgt tcccatcaga taattaaagt
     2221 gttgactttc tgcccaaaaa acatccatcc gattttcaaa aacaacctta taaatttttt
     2281 cagaagttat gacttctgtt gaggcttttt tctatcttga ggaacctttg ctctacttcg
     2341 gttaaattca taaaagaggt ctgaaaaaaa accaatatca atcaaatcac caaaccaagg
     2401 agcaagagag gctgtttcag cagtcagctc attctgtacc aagcgaccat tctcaacctc
     2461 accgtacaga caagggatga cttcagttag gaagttcatg aaatctggct ccataagtaa
     2521 tggcattagt ttgacctttt cttcatcagt taaagcagat aaacgaccat ttacaccagt
     2581 tgcaagagca agctgtgtgt aagctgtgag tgctttttgg ttgtcatcaa agaagttgcg
     2641 acctgtccgc tgttcataca tcttgatagc tggtaaagag taaagaaagc gcactgttcc
     2701 agtgtactct ctttcttcac cataactatc aaacgctgtg aatgatagtt cttttttaat
     2761 cattttatcc tcctggcacg atggctgttg ttcctaaagc ttcattgata aaatcaatca
     2821 atttcattgg ggtacttgaa gcgaacaatt tatcaaattt agcacggaca acacccttgt
     2881 ctgtatcacg ccatacaatt tctgaaacag gttttttatc tgaatctaga ataaaattgt
     2941 tgggtgacgc agtacatgga atttcaattt cttttggtgt agcagagctt tcatctgttg
     3001 tagtgctgcc ttttggagct gatgctttta cattggtcca gatgtggaac tcttcaacct
     3061 cagaaccaaa ctcgtctgta accgtttcag catatcccca aataaaattc gcattcacac
     3121 cagtatcgat gagcgctgga ggagttgaag ttgtcagctt tttacccaag tgatcaatca
     3181 tgaattgttt aggaatttga taagtcgtga tggatccctc agttgatttc ttaccttgaa
     3241 gacggacgtg ctccacgtta tctgcgtagt atgcatttga ttcttgtgaa gtttcaaaag
     3301 atgtttttcg caatcctgta aatggatacg ttttttttaa atcgagtgtg ccagattctg
     3361 ttttggaaat cttggcaaag aatcccgtgg tattaccatg agttacctct cgtgtgtcat
     3421 atttataagt cattgtgact ccttccttaa tttggtctga tttttattga tttcatatta
     3481 ttgagaaaga tttctttatt tttgaggtat gctggtctga tgtgttcttg aggtgctaca
     3541 aatccaccat tttttgttgc atggccattt tctaacaagt gagcaagcga cttctctttc
     3601 ccattgttat atactacagc gatatcttca atggtctcgt gagtccatcc tttttcatat
     3661 actccgtttc ttctaggact tccgtctcta atgtctccag cggtgctttt tcctgatttt
     3721 tctatgattt ctaaaacttg attctggata tcgattttta atgttttcgc attaacgcta
     3781 ccacttccca cttgtgaata cctcgattct gtaggttgta agtaagtaat ctgtatcagg
     3841 ctgttttaga ttcaactgac taggttcaca cataaaatta gacaacatca attcctcaat
     3901 gctgtctaat ttcttcttgt gatagtgact gatttgaata gtcacttttc tcatgtgtac
     3961 tgtgtcatca gcagtaatac tactacccgg agttaaacga tagtaaagaa taacgttgtc
     4021 aggagaggac ttttcctcac gttccatata gaacactttt gattttaaag tgtttttttc
     4081 taggatttct tgaatttctt gcctggtgaa gaacttctta gccattattt caattctcct
     4141 aattcaatta tcgtgtagtg gtcatcatca gattcagttc caacatttac cttgtactct
     4201 ttccctttgt acttcacgta gtctaaggaa tctgtcacat agttagaacg tatccgaaat
     4261 cttgctgtca aaacttgacc atctgccaaa gctttatcaa gtctacgttg gtagatcttc
     4321 tctttttcag ctttgacttt cttttctaca acttgttttt caaaaacacc tttttcgacc
     4381 tctgtacgct catcgtaaca aaggatgatt gatactctag atgatttcat gatttaactc
     4441 cgtaaatagc ttttaattga taaagaatat ttgtcaattc ttcatcaatc cagctccttg
     4501 ttgttgaatt tcctgtcatc aaggatttat caaatctctg aacacatctc aaatgtaacc
     4561 aatctaaaat tgtttcttta tcatcctctt caatctcatt ccattctgtc aattcgcttt
     4621 ctttatcgat gcgattgata ggaatgttgt ttctcgttag atatgaaatc ccactattta
     4681 tgtagcttaa aaattgagtg tcaaagattt cttcttcgac atcaacttca accatttctt
     4741 taatttggtt aaggattgtc attttagact cccctttcta tttaaaatca accttttgtg
     4801 aatttcacag ctgatttgta ctgaccaagt cggccaccaa gcacgctagc aagttcgata
     4861 tgacggcgat tcatcgttac atcataatct tcaaagcgat cagcagaaac atcatcacca
     4921 atcatcttat aagccttgtc agcaaatgcg ataattgggt tagtcgcatc ttccatccag
     4981 tcatagacat atacttggta accagcaatg acattccctg tttgtgaaat tggtgcgaat
     5041 ggttgtgggt caatgtagcg tttttcgcca tccttaacca ttttaagttt acgagcaatg
     5101 gtttttgaag ttaccaaaat tggagttgta tttgcagcaa gtttatcaat ccctttgacg
     5161 aggttttcta aaacagtact gtcaaattcc ccgtcaacac tgatttcttg tgtatcaaat
     5221 agttgagcaa gtgtttcttc tgcgatagat ttaatttcag tgattttgtc atcatcatca
     5281 ctatttttac catctccgat aacaacagca cgttcaactg cacggatgaa gccttgtgct
     5341 agttcattca tcacatagtt gaagtaagca cctgttgtat ccttcttcaa gtcagcatac
     5401 tcaaaactgt acttgatgta gacagctgca gagttgattg tataatcgat aaatacaaaa
     5461 gattcatctt tctttgtttt gccattttga tggcctttag cttttgcttg ttgcgtttga
     5521 agcgcaacac gtactgcata acgaggatct ttggttacat ggttcaggat accatcgtaa
     5581 tcattaaatg cattttggat tgcaatcaat actggttcag gtaagatttt gttaacatca
     5641 gttacacctt tttcaaccag atttgcttcc cacgctttac gggcactgtt tgagcttcct
     5701 tcattatcca taaggattcg agcgaaatca agtgcagctt cttttgtttt taagtattcc
     5761 atttgtatct tgcctttctg tacttccttg atagatttag cagctttatt gagattgtct
     5821 tgtttttctt caatctcaac atctaactta gaaattgtgt tcttgagttc atctgctttg
     5881 gataccaatc cttctgcatc cgatttcaac tgtgcaagtt cttcttctcc aatagttgct
     5941 gactccaatt tctcttcgat tgaagctttt ttaaatttga tctcagataa ctcatctgca
     6001 tgtttttgtc gttcttccat caattcgact agtgttttca tttttttctc cttttttaaa
     6061 tcgttgcaag tttactcatg atatcttgct tcatgttcgc ctgagcgatt cgcttgtcaa
     6121 ccacagacat atcaaatccc ttaatattat caatggttgc ttgaggattg gctggcacgg
     6181 tcacgacaga tatttcaaag atttcaactt ctttaaaaat ccatccaccg taaggttgct
     6241 tagcgtcaac tggctcataa tcattaataa aaaatccaat gctcagacta tccagtgccc
     6301 ccatcttcat gaggtcatag gttttcttag cttctggatc gcttagattg aatgttgacc
     6361 gtgttcgcag acctttttca tctaccgaca gctcatgctt accgatgacg cgattgcggt
     6421 cgtgatttaa gcacataggg acgacggcct tagttttcag ggtattgtca aaacaccctt
     6481 tggccatcac atcaccatct ctgtcggtat tgccataggt ggaggcataa gcctcaaagt
     6541 gaaagtcagc tgactcttcc tcaactgact tgacgacaaa ggtttttaat ttttccatat
     6601 cctacctcct ttcttaaaat ttctgccaac cgcccaccct atttttaatt actttcgctc
     6661 ggctcgatac ggactgcatt tagattggtt tcgaatactt ctccaccttc atatcctgga
     6721 agccctagat aggtttcacg gaattcattt gaattcatca aacctgcgta tttagattta
     6781 aatccacctt ctactagatc cttgaatgaa atcatgtcag ccatatcaaa gaagaccaag
     6841 agcttgtttc cttgtgtcct tgccgtcttc gtgaaatatt ttctattaat ttcttcagag
     6901 aatacacgtt gatataattt catgacgcta gaatagtaag ctctatattg ttcttctgtg
     6961 tagtcacaag taaacaattt ttcattaatt ccatgagcat gataaagttg agatttcaga
     7021 aactccattt cttctttaga agcggttgag taatctttgt ttaattccat aaactcttca
     7081 ccttgctcga gataggcaat gccaccaatt tcagcaagtt ccatcatgct atcaactcta
     7141 ctcttagctt gtttcttcaa atgttcatct gctgctttag ttggtagttt taagaatcct
     7201 ctcaactttg aattccctct gcctaacttc tcggttaacg catcaaggtt gatatcaatt
     7261 aattctgtga tttggtttag ttgacttgtc acgtttaatt taggattttc aaaaacccag
     7321 acatcgctaa gaggtagctc aatctctaca tcatcaatca tgatttcaac tctctctgca
     7381 gtccatgata tggttttctt tgcaaaccaa atttcaatca gtcgaccatt ttcccaacgt
     7441 ggaacaacga ctgcaacacc atctttcagc atagctcttg ttacatttgc ccaaaataca
     7501 actggtattt caagaggatt tggagagaaa gataaaacat ttgcaagatc actattttca
     7561 aaccactcca tcttatcaac tcccgtcgga tttcgagtga ttctcacatg cttaaatcga
     7621 acttgtgcag tatctgttga aatcttattg tagatattgt ctaagtaaat cgaatttctt
     7681 ctccaataat tcaaatttct ttgtaaatag gtccttgtgg attttctatt gcttggtctg
     7741 aaaatcctag caaaaacctc tcttaaatta tttatatatt tgttcattct tcacctcaat
     7801 caaagtaata actcaagtct tccttgaaat tttcgtagca gataaaagca tctaactgac
     7861 tagcaaatac gtcaatcttt tcttttgctt tttctttatt tggaaataca ttgttattcg
     7921 catctatctt gacacgaaca tttgcgtggt tccaagttgc cacaggatcg tcaaagatga
     7981 ttttccccat cttagctttt tctttataca cttttaaagg attggataag ctcttgaccg
     8041 tttgtggaat gtcgtgacat atatctccgt agtagtcatt aattaagcgg ataagctctt
     8101 ttgcattcca gcggtcatat ccgactgcaa ctggtaagat tctattctca ctcataaact
     8161 gccttaattc ttcaaagata taagcttgat cattgtagtc caactcatga acatgaagct
     8221 gtccactaag ctcccactca gcgtatttgt ccctcagttc tttcggaagt ccttcaatcg
     8281 tatgacgtgg catgaatttc ttgttcaaat actgacgctc ttcgccacgc acgaccataa
     8341 atgagaccga acaaatgtca ttgacatccg acaagtcaac accaagcaca cagcgagcac
     8401 tcctctcctc atttccgaca aacaaactct tatcaaactt atctaaccaa cccttacact
     8461 cttcattact gaagtaagca agatagttat taacagggag attaaatgtt ttagccatca
     8521 gctcagcctg ttgtgctgga tcattcttgc tcatttcaat atccttggca atcgtctcct
     8581 tctcagtcgt tataccgagt aaaggcatag ctttctgcca catatctgga ttgtgaattt
     8641 cagaaacatc atccagctga taaatccaag gcatgaccga atgattgaca atcttttcat
     8701 caagaatatc tacccagatg ttgtaatact tatcaaaaag cttgtcccgt ttcgtcccat
     8761 tggtggagat gtaccaggtt atccaatttt ttcgcttgcg actcgaacca tcattcacaa
     8821 ccttgatgaa gtcatcatca taagtgtgca cttcatcaaa aatattgtag tgagcattag
     8881 taccatcaag actttcatag tcggaagtct tgattgacat aagactatta gttgtctcgt
     8941 acaagatacc ttgtttagtt gaccgtagta tgtcagcctc acgcatatag tgtagcaagc
     9001 tctcttcgtt cgacagcata gctctagaag cattaaacag atatccagct tgttcacgac
     9061 tgtaagctag aagctgaata tcagcacccc actcaccgtc aataatctga ccaacctcac
     9121 caatggcaga accaagggtt gtttttcctg taccacgagg tacaataata ggcacctcat
     9181 gaatgagacg cctttcttca aaatctttat attcttcaag tgtatcggga tctgtttttg
     9241 taacttcaac tgtatgataa aaaccccacg ttgtttctag ccagaccttc tgaggcaaag
     9301 ccaagtgtaa ctttccagca agacctttag tgttgctgca ctcttcctca atgaactcaa
     9361 tccgtttgtc aacttcttct tgtttaaaga tgtattgttc cttgtacctc tctactcgtt
     9421 taatcgactt catcgtaagt tcacaaacac gaatcttccc tgagtagacc agctgagcat
     9481 atttatcaaa atatctcatc tcaaccatat cgagccaact tctcctgaat catttctttg
     9541 aggctatcac cctgtggact ttgcttttca atcgttgaca taatctgcat gtttagcttt
     9601 tgatactttt ccattccatc aagtaaatac ttatcaggta gctcaccgtc attgatgact
     9661 ttattgattt ccaattggaa gttttcaatc actttttgat tatgattgta ttgagtttta
     9721 agatttttca aacctactga atcattgtca ttgatttcaa gcattttttc ttttggaatt
     9781 aacttgaaag tcttacgaga tagttcaaca cgttcttctc ttgtatactt ctgccgttga
     9841 tttgccagct tttctaactc tttgaattga ctttttgtga tattcgaccg agtttcttca
     9901 aatatgccta gtttttttcg atacctggtg agggtagcac gacttattcc tagcttttct
     9961 aaaacttcat tgattttcaa aatcatgctc ctttcttgta tcaattttcg tcatttttgg
    10021 gggagaggta tataagagga ttgacaccgt tattattttg ggtgtgtgaa aattttaaat
    10081 aggggggatc tgataaaaat caaaaatcca aaaaataaaa aatcaaaata aattaatatt
    10141 ccgattttct aaatttaaat ttattttact ttgaaatgtt tttgtattat gacactcgag
    10201 acaaagtaat tgcagattat cttcgttgag agtaatagac tcatcttgat aattagtttc
    10261 atcaatctct ataatatggt caacgatgct cttgccatga attaaacgtc cacacacatc
    10321 gcagcgcata cgctttgtag ttctgattct atttctcaga gttctccaag gtttcgagtt
    10381 gtagaattta atctgccaag atttaaacca gtcagagtgt ttaggattct tgaatgtcat
    10441 agcttgaatg attcaactcg gatttctctt gtgtatctgt tcatgaacga aatcatctta
    10501 tacattggct tgtcccatgc ataaattatt gatgtttcaa ctagaatctc actagtcaac
    10561 gagtacggat acctttttgg tctcatgctt acctccaaca aaaagagaac atatctagac
    10621 ttgctctccg aatttttcgc atgatacaaa tatatcagat tcattttgtc aattctatat
    10681 ctttttttga caagatttat ttttgagttt tgaatttatg taaaatatct ctgttgaatt
    10741 agttatatct tatattttat ccaattttgt ttcatactaa aaaactagca cggacaacgc
    10801 ttcaggccct cttcaaaata taaactagaa acctcctcgt tatggatagt tgaaaaaatc
    10861 aaaaaaatat tagaggctaa aattactcat cttagtatca agttcatctt gccttacaca
    10921 aatataaatt agtgtaactg ctggacttga atgattgaat aatgacatca agtctgcaac
    10981 gttcttgtac ttcttgtagt aatgatagcc aaatgttttt cgcatcgtgt gagttccgac
    11041 attatcgatt cctaagtctt cagcagctct tttaagaaac cagtataccg tcttatagct
    11101 gagcgcctta ttctttccga cacgactctg aaatagatac tcatgtaatt ctttatcttt
    11161 gacaaattcc ctcaattcat tcttgagtgg ccttgtcatt ttaatgctct tgtatttccc
    11221 tgttttctgt tccctaactt taatatgcca accttgaaca tctttaacct ttagtttgag
    11281 aatatctccg acacgaaaac ctgtattgat tcccaaaaga aataacatgt aatacttttc
    11341 attccaagat gatagatagt ccttcatggc ttggatatca tccttatctc gtaacggttc
    11401 aacaatattc atagttttac tcctttcaca aaaaaataaa gcactaagat tttctcagtg
    11461 ctttggatag tatcaatcta tcatattctt tttgtcaatg ctatactttt ttttgacaag
    11521 ttacatgaac aataactttg caagtgtatc aagaatgatt tcacgtcttc tgtaaatctg
    11581 cttactgtgc ctgtatagat acccagtgtc accattctcc attttatgcc aaacttgaat
    11641 ccaatcgtac ctagtgtgtt ctccccattt caaatgaaag atttttttat catcaggttc
    11701 aagtacatcg agtagctttg aaattgctgt ttgaaagttt tccagtctta aaaccatcgg
    11761 atcgcttgca taagcaactg ctaggttctc agacctattt acgaacgtgc cacttccgct
    11821 tgcaccagta tcatcaatac caggaacagt gagatgtttc acttcgtaca atcgttctaa
    11881 ttcatgtctt ctttgaccaa tcaatttgtc aatctgtaag tatttatcat tgagttcaaa
    11941 ctcgagataa tctcttcgtg ctgttattag gttctttttg cccaaacctt acctcccata
    12001 tatcttttag ttttgaccca cttgataatc ttaccttcgt tattgtttgc gaataaatcc
    12061 aagaatttca caacatcacc tcatccccaa ctttcacttt ctcatacacg gtcttcaaaa
    12121 taactatgaa atttacttaa attgacaata gcgacctctt caacaaaatg cttttcaatg
    12181 tcaaagtctg ggtcattttt actaaactct ttctttatcg ctttttcggc cagcgaaggt
    12241 aaagcgaata tgcttgctcc gttttttagg gccagcgctt gaccgtgttt atttactatt
    12301 cgataaccaa catcaaacgg tctaatttcc attgggattt ttatgcgttt actttgattc
    12361 ttcattcctt cttcaagcgt ttgtatcatc actccacctc ctcaacttca acaccctcac
    12421 attcgacctc gtagccgtca agccatgcac gagcaactaa atcaaatggt ctttcttgca
    12481 taaaccattt cccaacttca tcgttcgtga aatcaaattc gaataattct gccacgtctt
    12541 tacaagcttt tctagcttcc tcaatccatt cagccacaaa ctgcggaact ttgactttct
    12601 gcggttcgtc tagttgttcc aagtctctta gaacggtttt tagaatagtt tttccgctaa
    12661 caatacctac aacacattca accgcttcgt acttcttaat caatgcctta atattcatct
    12721 tagtttcctc cataaatcaa ataaactgca ataactacct gagccatgct tggcgaatag
    12781 ccaacccaat catcaaactc cttagatttt ggcaaccaac ccttagtagc tcccaaatca
    12841 tagtcggtag gcttttcatc agcgaagatg cattccatcg ctcccataaa tgtcatacca
    12901 tcttctgcca tttcccgaaa atagtccgcc cggtctttca ctgcttgtgg taaatcttgc
    12961 tggggaggtt gcggcttccc gtcttctacc gtccagttgt atacttcatt aacttttttc
    13021 tttaactctt ccatcatctt ccaactcctc attatatttt tctaccaatt catgcaacca
    13081 cgaccaaggt tcggtttctt caatgattgg gtcaacttcc ctttcttgca accaagctga
    13141 gaaattaacc acattatcaa tgtagattgt gtcgtaatcg ccccaatccc aaacagttaa
    13201 ataaatttct gtttcagttc cattttcatc ttcaaccgtt attgaaccat tttcaaccca
    13261 cgctgtacca aaacacaatt cgcaagtgcc agtctgttct tcttgaaaat ctgaatggta
    13321 ttctgtcact ttatatttca tcttccaact cctccacttt tttcctcaat tctttattct
    13381 ttttcttcaa caaatcgcgc tccagcgctc taatccgtct cttgcgtgca tcacacggct
    13441 tcgaatactc gattatcttc tcttcgtttt gctcgattgt gcgtttcagt ccatcaatga
    13501 ctgttcgttt atcataattc atcttctaaa aatctttcaa tagcttctct gtaggagact
    13561 tccaccagac cgtctaagtc gttcagggct tcaatatagt ctggacgacc ttgcccgtac
    13621 tgctctttca aaaattcaac aaagagatga atttcctgat aggttactcc aaccatgttt
    13681 cttacctccc actaaaaatg taacgttatt gatcatcttt cttttccttt cttgctgcac
    13741 gttccccgac taaatagccg agaaataacc acagaatagc cattccaaat tctttaatca
    13801 gccacctcca ttctaaaaat attgcttccg cttgtttgtc aagtcgttga aaaccattaa
    13861 atggtcttta tccacaccct tcatcagtct ggacataaag ggtctgccat atcttttctg
    13921 aatatcagca gaagtcaaat tggtggtaat gattgtattt gaacgcttat tcaggatatt
    13981 gtagagaata gtaaacgacc attcgctatc cttttccatt cccaaatcat ccaaaaccaa
    14041 gaacttagca ctggcaattt tattgaccag aaactcctcc tgactaaaat cagctttaat
    14101 cttcatcaac aagtccgtca cgttgatgaa gatagcaatc tctttcgtgt actcagatag
    14161 agccttaacc atcgcaaagg ccaaatggct cttacctgtt ccagcttctc cttgtaacac
    14221 gatgttgttc ctagcaccct cagaccactc acgacaaatc ctctttgcaa aaactagctt
    14281 ttctgcttct ttttcagttg gtgtttcaaa attgtccaaa gtcgcatttt tcaaaacctc
    14341 atcataaaga gaaaacttct caagatagta tttcctctct cgctcattct cagcattagc
    14401 cagttcattc actctttcct gattttcttc atgaatccgc tcagattcac acatgcgaca
    14461 tacaacactc tcggtcctca atatctttat caaagggatg ttatgctttt cgcagaactc
    14521 ttcttgttgt tctgtattcc tacgataaga taaggcgatt tcctcaaaca cattgtctac
    14581 catactagcc gacctccgca ttcatgccag ctagccattt cagacaagca ggcaaccact
    14641 tgatgaattg gttggtctgc taaaagagtt ttcttctcgt agcttaacgg ataatagtca
    14701 atctcgaact gctcaattag ttctaaaatc cccattcgtc ccttacctcc tgttccgatg
    14761 ttttatcgtt ttgttgtctt gacttcttac cctctttata tttacggtcg tcctcatcca
    14821 cctgctcaat cgatgtgaag cctttctttt tccaactttc aagaatggct attaggtaat
    14881 tgaaactagg tttgtgtgat ccggaagttt tctcgactgc acggttcaac atatcgaaac
    14941 tcattccatc aagtcccaca taatcaagta actgttgatg tgacttatca gttagatgga
    15001 ttccgctatt tttcaaattt tcagaaaggc tggaactaat catcacctta ttatttttat
    15061 ctttctctat ctctttatct atatctatat ctccgttgca agttgttgcg atagtgttgc
    15121 aatgcaactt ttgtaattct ctgtgcttac gacttctacg agtactcgct gtttcactcc
    15181 caaccatctc aggaacctgt tctaagaaat aatcacggtc agagtttcta gtcagcaatc
    15241 ctttgctttc taaaaaaatc aatgtaattt taatatcttc aacattttcg tcaatgacaa
    15301 gagcgatttc ttcggctaga ttgtcagcaa gtccatcata gtaaatatgc cctccgtctt
    15361 ccaagctaat taacatcatt ttgagataaa tgatggtatg tgtatctccg ccagcaatct
    15421 tacgaagtag tttcatctcc tttgacttaa agaagtcctg agcaagctgg atccagaaat
    15481 accgcttgtt ttttaacgcc ataatctcac accccacttc ctacagttag ctcggtactt
    15541 catccgcata tcctcataga tgtgcctgcc ttccagctcc attttttcaa tctttagcag
    15601 cttattttta agggtcacat aacgatagtc ctttgctagt ttttcatagt cggttaggta
    15661 ttctttgact agtaatagat ttttataatc gttttcccat atagtaataa aatgtcttga
    15721 agttgattcc cttccttcca gttctttaac aatcataatc aggttatcca gcgattcaat
    15781 caattcttcc atttcctgac ctcctcatta caaaaatctg attgcagact gtttaggttc
    15841 tggcaaagct aacggctcag ggcgcaatcc tacgggcggt tcgttgtcat atgtgaatcc
    15901 tttgaactct ctgcgaatat tcttgcgaat ttcttgccat ttgtcctctc taccacgttc
    15961 atatgcatga ttacgcactt ggataatcat agacgcaaat tcttgctctt ctcgtctttc
    16021 ttcttccttg cgttgttcct gcaatttgat atgacggcaa gcccctgcaa atccaagcag
    16081 caaggctcca acccccatca gctggtctaa aatcggtggt tcaaacattt ttatctcctt
    16141 atcctctttt tgtgctataa tatagtcaaa taattttgct aagaccttgt ccagaagcct
    16201 tttagtaaag ttattatatt tgattagaga gccattcttt gatggctctt tttgaccatt
    16261 tcttaccagg taattccttt ggaaatccct ttaagtaacg ataattatct gaaaaggtgg
    16321 catacttaat tcctagaaat tcgcaggtag tgttcacatc catcaactct ggatagtggt
    16381 cactatcttt ttctatttca actaatctag tgattgtatc cttgataatg gatttaatcc
    16441 attcagatag tgaaagtaga acattgtcca tctttttccc ctcctaccct tcgtcaaatg
    16501 agttcaattt catgattttc atcttggtat tggtgcttgg ctcccaagtc atccagtaag
    16561 ctagagcggc atccgcatgc ttcttgggta gcaagtcata gcgactaatg ttgaagtggt
    16621 ctttaaagtc aatctcagct tgtctaaaga ctgactgagc aaaaatctta tccgcataag
    16681 cagggctatc aataccaccc aggcaagcca caaccctagc cttacgcttc ttcagtaatg
    16741 attgggcata gctaggatga atcggttgct cactcttgag gtagtcaata tcttctagca
    16801 tggtcgcttg ttgctcacgc aatttctttt ggccagtgaa tagagcaata aaggcatcct
    16861 cgtccaaatc ctcacgtatg aatccaccct gcttgcgaat agctggcaaa acctctgatg
    16921 taacccagcg cttgaactct ctagcttgta gaagcttact tgaaagaatg agagagtaga
    16981 gaccagattc gttaatgatg atagggtttt gatttctacc catggcgtca cgaatcgtta
    17041 ccccatctgt cttatcgtca tcatctacat ggtcaaaaat tgcttttctt gaattcgcat
    17101 atcccaagat atccgctaca tccttcccaa cgaaccacgg ttcgtcatca attgtcaaag
    17161 tacggacttc ctgcccgtga aaattaaaaa tttcgttcat aatattcctc ttcttacttt
    17221 tcctagtgtt aaaatagttt cccaaacatc tagtccctca agactatcga tcatcatctg
    17281 actaagttgg tgatttttct tctgccaatt cagtattatt ttcgcttgca tgtatggacc
    17341 tctcagtgat ttctccaagg gttttcaata cccaaaacat ctgcaacttt ttccttaaca
    17401 gactcactac ctttgccata cttcagcaac tctgaaataa ctgatgatgt tacaaatact
    17461 tgttttgcta actcagcttg agtcatatca agctcaatca aacgagtttt gattttagcc
    17521 ttgattatct ttagttcttt actcatcttt ctcctttcta tttcttcgtt tcactttcca
    17581 gcgctctgag ttctatctca tggctggctt gtttaaatag cttctcacac gctattttag
    17641 cttctctgta cgttgtgttc tcgctgatga agtaatcagc aagttcgatg attttatctt
    17701 ccattcaacc tcctatatca gtctcaagac tgaggtaata tcttcctaaa ttgctataat
    17761 aatcttgact aggacctctc accgttttag tcaaaattcc aatagaaagg aggaaatttt
    17821 atatgtcaaa actcactaaa gaagatgttt tacaagtttc tcaagaaatt atcaacgatg
    17881 ctattccagt tatcaaagat atgttagatg aggtatttaa agaataccca atcgacatgg
    17941 aaattagaaa ggctattctt aatagcgtcc ttgtcgctca taaactcagt acagaaacta
    18001 cggtttcgtt gctaacagaa cttgtaaatg ctcaagaaaa ctagtgtttc ttagaatttt
    18061 ttctactaat tcagggtctg cctttacgag ggtggactct tttttcccac tatacggata
    18121 tcgtcttggt ctcatttcct cacctccttt taaaaaatta tctaaaaagt tagcgaattt
    18181 cttgacattg ataaataaat ttattaaaat caaaacatag agaaaagacc tactaaaaag
    18241 taaggttata cctagaaaac ggacgccaat cagtttcatt aggctttatt ttttagttgt
    18301 cttgttcgct aactctttag cttacaaaaa ctattgtagt aaatttatta aactttgtca
    18361 acagttttgt agtaaattta ttaaatattt tttgtcatgc cttagaaagg ttgatgtatc
    18421 aatgtttttc acatttgaaa aaataaaaga attggctgac aaacaaggta tttcattaaa
    18481 taaacttgaa gaaaaactag gttttagcag aaatacaatt tataacatga agaaatccac
    18541 accaaatgtt gaacgagttt caatgattgc tgactacttc aacgtgtcca cagattattt
    18601 acttggtcgc acggataacc caacaatcgc tggtagtcat gactacaaat gggaaggtaa
    18661 gactctaaac gttgaagaaa tggcatctaa tgtcatgatg tttggcggtc gagaattaac
    18721 agatgaaaag aagaaaatca tccagtctat cattgaaggt tatctcaaag aagctggtga
    18781 ttagaggtac tgcttagtga ctgaaaaaga aattataagt cattttcaga ttcgtattat
    18841 cgattttgat ggagatttga tgcctgatga acttggattt tacgaaaaag aaaccaatac
    18901 agctttcttg tctaacaaac tcagcaagaa agagagagtt aaagtactac tgcatgaact
    18961 tggacacaaa gaccacacac gctcagagta ccagaacgct cgcctacgct gtgaaaacga
    19021 agctgatagg aatatgatcc atcatctcgt aaaagacgcg atagaaagct tagacgaccc
    19081 cacagagttt gattacctca aattcatgtc ctactacaat ctaaaaacca tgacgaatga
    19141 agtcatggta aaagaggaat actttgcatt gatggagtga aaggagactc atatgtctta
    19201 ctcgtatgtt gctttagatg ttgaaactgc gaatgacttt cgcggtagtg tttgttctat
    19261 cggattagta aaatttaaag atggaaatat tgttgatgct ttttacactt taatcaatcc
    19321 agaagaagag tttgatgatt tcaatatttt catccatggc attacacctg aagatgttct
    19381 tgattcacct acattcccag aggtgagaaa ggcgattgtt gattttattg gttctgatat
    19441 agttgtagcc cactttgcac agtttgatat gggtgctctt aaagatgtat accaaaaata
    19501 cgagctggat tttgataata tagaatatat ttgttcgtat cgattagcca aggtcgctct
    19561 ccctggacaa ttgaattaca aactaaaaag actagctaaa aatttgaata ttgagctaga
    19621 ccaccacaac gctttatcag atgcacgagc aagcggattg attttagaat acttattatc
    19681 tactaattca ttttccgacc tcaacgcttt tttaaaagaa tatagctaca ataaaactgg
    19741 cttacttggt cagtatggat ttaaaaggaa aaaaagttat cagtacaaag aaaatcttat
    19801 ctatcagcca acagaagaag aaaaggcagc aatgaaccca gaccattact tttacggttt
    19861 gtacttttgc tttactggaa aactcgagcg aatgactaga aaagaagcta acaaagctac
    19921 tgcattagtt ggtggtattc ctgaaaaagg agtgactaaa cacactaata tcttagttgt
    19981 aggagaacaa gattggagag tagtcggtac agatgggtta agtagtaaaa tgaaaaaagc
    20041 acaaaccttg ttagaaaaag gtcaagatat tgaaatcatg acagaaaatg attttataag
    20101 attgcttgag gagtaattaa caaaaaataa caaaaaagct ccacaatctc cctcgccaaa
    20161 gtttgattgt gaagcccacc cttataaaaa atcagccatt aaaaaggcct cttttctata
    20221 ctctatttta caccatgaaa ggggtgatgt caatattctc aatgtttaga ccttgtccag
    20281 aagctgataa acaaggagaa tacaatgaaa tataataaaa caaaataccc aaatatctat
    20341 tactatgaga ctgctaaagg caagcgttac tatgtcagac gttctttttt cttccgaggt
    20401 aaaaaaagag aaaaaagtaa aagtggtttc acaactctcc ctcaagctcg cgcagccttg
    20461 gtagagcttg agcaacaaat tcaagaacaa gaattaggta tcaatacgaa tctaacgctt
    20521 gatcagtatt gggatatcta ttctgaaaag agattgtcaa cagggcgctg gaatgacact
    20581 tcctactacc tcaatgacaa tctctataag aaccatatca aggcaaagtt tggttctatc
    20641 ctgcttaaaa atttggatag aaatgagtat gaactattta tcgctgaaaa gttgcagaac
    20701 cataccagat acactgttca aaccctcaat tccagcttca tggcattgct gaatgatgcc
    20761 gtgaaaaatg gtaatctgct ctcaaatcgc ttgaaaggtg ttttcatcgg ccagagtgat
    20821 atccctgctg caaacaagaa agtgactctc aaagagttca agacttggat agcaaaggca
    20881 gaagagatta tgccaaaaca attctacgct ctgacctatc tgacaatttt tggattgaga
    20941 agaggagaag tctttggatt gcgtccaatg gacatcactc agaacgacag cggacgggct
    21001 atactgcatc ttagagacag tcgaagcaac cagaccttaa aagggaaagg agggcttaaa
    21061 acgaaggatt cagagcgata tgtctgcctt gatgatatcg gaacagacct gatctattat
    21121 ctgatagctg aagcttctaa gattaagcga aagttaggaa ttatcaagga acagcacaag
    21181 gattatataa ctatcaacga gaaaggtggt ctcatcaatc caaaccagtt aaatagaaac
    21241 ttcaatctag tgaatgaagc aacaggattg catgtaacac ctcacatgat gcgccacttc
    21301 ttcacgactc aaggcattat tgcaggggtt ccgcttgaac aattaagcca agcgctgggg
    21361 catacaaagg tttatatgac ggatcgttat aaccaagttg aggacgaact tgctgaagcg
    21421 acaacagacc tatttcttag tcatattcgc taaaaaaatc cccaccaaaa tctcaaaaag
    21481 tccccgccaa ttccccgacc aaaatccgaa aaataccgaa aaatatcgaa aaattatttt
    21541 tagaatagtc cccaaaagcc tgaaatagag ctaaaaaact ccacctgatt cggtggagtt
    21601 aagggagatt attatgaaaa aggtaaaata aaatcttatt aaatcaacac tcttggaggg
    21661 tgtcccctcc aactccccga cctctggaca aggtctattt ttttgaaaaa acttcatcaa
    21721 atcgcttgac tttctcggtg caccgtgata taatataatc aagataagga aagggggtga
    21781 tgaaattgaa caaagaagat tggctcaggt tacttgaaaa ggcaatagac aatattcctg
    21841 aaacagtaac tgctatcgca agtctagtga ccgcaataac ggtcgcaaga caaaacaaaa
    21901 agcgtaaacc gaaatcccgc aaaagaaaaa ggtaaacgct aagaggttgg ggcgaaagcc
    21961 cctcacgcct ctattttatc aaatggaaag aggaaatgca atggtcagtg caatagctat
    22021 ttttataatt gtaatcaatg tatatattta tctaaaaaac aaaaaggaca aataggatga
    22081 ggaaagttat tgaagaattg ttagacagtt cgatgtctac atctgctatt tcgcaaggtg
    22141 ctggagttcc atggactact gtttctgacc tcagaaaagg aaaaacaagt atggacaaaa
    22201 tggctcttct aacggcagaa aagctctatg aatttgctac aactgataag cagtgatttc
    22261 gatcactgtt tttattttta acaaaacacc tccctatttt tcatgtaaac gtttttttga
    22321 acaataggat ttagatttct gtttctaatc gttcaaaatg cgtgtttttg aaaaatagaa
    22381 cttaactcta attttttagg caaaccattt attttgctac cgacattggt gtcggttggt
    22441 tagacgtttt cttgattcag acaaacaaaa aaaccgcaag cctgagcctg cggtgaaaga
    22501 acaatttaga aagtttcctt tctatttatt taactgtaat caagccatct ggctctactg
    22561 tgaactctgg cttgtctgcc agtgttccgt ctggtttgag gtagtaccag cctgttccgt
    22621 ccgctgactg gataaaggca tttgatacca tggcgccttc tttagcgtct aagtagtacc
    22681 aagtgttctt gtacttgacc cagcctgtct tcatggcacc ttctacatca aaatagtacc
    22741 acttctcagc gattttcttc cagcctgtgg ccatttcgcc tgattggtca aagtagtacc
    22801 aattaccgtc tgtgtgcttc ttccagcggt ctgaaagcat atagcctgag ccatcgaaat
    22861 aataccaggt accgttgatt ttctcaaact tatcttttgg ataagagccg tctgagtgta
    22921 cgtaccagta gccagtgcca tttttctgcc agcctgttgc agcgctcaag ccgttttcga
    22981 tgtcttgctt aaactgctca cggctaatgc cccaacttgc aagatatgga taagggtcaa
    23041 cgtggtctga gtggttgttt ggttggttat tggtacagta ttcatgcgtc ttgatacctg
    23101 ccaagtcgtc tgtatcaaga gtcttcggca agcctgcttc gtccgctaga ttgcgtagca
    23161 attcgatata gaggcgatag tcagccataa actcttcctt agttgaatgg ctttcaatca
    23221 gttcaaccgc tgcgtaactc tcagcattcc aaccgccccc aacatcccaa cttccgttgt
    23281 tcacaggtcc tacctgcatg atgcgaccgt tcccaacaac gtgcgagaaa aaacctaatt
    23341 ctgggtcttt ccgccagtga taatccgctt cattctgtac ggttgagtta cggttgcctg
    23401 ttgaatgggc gtgtacttgt cgataaggtt gcaccccaac ctgtggcaag cctgtacgta
    23461 gtctgtttct atcgatatcc attccctatc gtcctttcca tgcgtcattc atctgcttca
    23521 ctgctgactc tacgaaggtg tctaagtctt tgtcagtcat gctaatattg tattttgtaa
    23581 gctcagcacg gactttagcg cgagcctgtg ccagtttttc atctcccttg tagccagttt
    23641 cagcagctac ctgctccacg gcatttactg catttttagc tagaatttca gcgatgatca
    23701 ccgctttctc tccgccttta cgcaaaagat aatcttttac tgcttttaca ataccgccta
    23761 ctgctactgt taaaaagctt gtcgcaaaag caatgatgat ttcagtaatt tgctgcattt
    23821 gttattctcc ttttttcgtg tcatcatctt tttcaagtaa tcgctgaaat acttttacaa
    23881 tcggctgaaa aagagtaaca tttcctttta atttgcgata attttcaatg agagattgaa
    23941 aagtaaatgc gatgtacccg agatagattg agtgcaagaa tacaaaacct gtcttttcag
    24001 gcaacaaaac ggacgccgga atgaggatca tcagtaagag aacccctgaa atcttacgaa
    24061 ggagcccgtt aatgccgatt ttgctcttgt actcgatgtc aggattgata atcgccgcaa
    24121 tcgtccctgt cacaaaatca atgatttcca ttgagacaat cagtgctaga gcgtacaaga
    24181 ccaaaccatc ttcagtctgt acgacacttc ttagaaaatt gaaaaattca atttgcatac
    24241 acacctccta ttctttaggt tttaccgttg gatccgtcca gtcaggattg ccctctgcat
    24301 caaatttcat gatatagaat tcatgattca acagaacggc tacgttgatt gttgcgattg
    24361 taccacccca ctggttgaat gcccaaacgg tttcaacatc cttgaattgg cgacggccat
    24421 ttacgatcac aggacgtttt tgaacgtcac gatacatata gaagtcatcg cttacattct
    24481 tgcaacgaat gaactctcca ttttctttca tgtagcgcaa agcactcgca agatcaaatg
    24541 gttctgtgat ttttgtaagg tctagtaagt tatctgtgtt ttgaattgtt tctgccatgt
    24601 ctattctcct ttgtcagctg gtttagtttg ttcatcaagc agagcttcca gctcatccac
    24661 tcgtgcttga agtctttgat tctcttccct ttgctcattc aactgaatac tcaagatatt
    24721 acttgtaatc atcgaatttg ttgaagttgt tgacatttca ctaattgtca tttgtaaggc
    24781 ttggttaagc tgttctgtgt tcattttcta agttctccaa tctgtgtgtt cgttttctat
    24841 tttcaagagc aagctcctga attgctttaa agtgcgatat tggtcaacat gttgttattc
    24901 atgctattgt tttctccatt ttttctattt tttgatttaa ttcttgaata gccttgatta
    24961 agtaaggaac taaagcggta tagtcgatat gcagatagcc atctggattc tcaggatctc
    25021 gtgagacaat tcttggaacg atggtttcag cctcttgagc tattagacca atctcctcat
    25081 gtttcttatt ttcgatgaaa tcaaatgcaa ccattcttaa tctgttgatt ttatccaagg
    25141 ctttcacagc tgtatctgtg atgttctctt ttaagcgtct gtctgatttt tgttccatcc
    25201 aatacttcac gctaccgcta ccgacctgat tccaccaaac aaccgcattc cttccgcctt
    25261 tgggattcca accatcacca agcacatctt tacttccaag ttcgatacca tttgaaaaca
    25321 caggagaacg agaaaaagta gtattcccat agaagtttgc tctcgatgaa ttcgaaaaat
    25381 ccacttgatc ataaaaaccg acttcattcc tacagtacat tttcccatca gtattgacgt
    25441 tccatgcttt aggtccggca tagttccaat tatttcccca gttcgcccag aaggctgtcc
    25501 ggactccata cccggcacca ttccccatac caacagagaa ctgattgaca cctgaaatcc
    25561 agcgaccgcc accctggtca aattgaccaa gtgtgaatcc accgattcgg ccttgatagg
    25621 cttctaggaa ggttgagcta gaaatgacgg actcaacctt agtagagaag atacgtttag
    25681 atgtcagtcg gtcaataaaa gcatcttttg cagttaattt tctaataagc gcatcgtcaa
    25741 ctttcaactc ctcagcagtt accgcttcag cgtctaatat cgtagtcgtg accgaaccag
    25801 cttcaaaatt ggccgttttg agcttatcaa ccatggcaga cttgatgact gctctgtcaa
    25861 tcagggtctc tccagtgatg tgggtcaatt tcccaacaaa gcggttatgt ccattggcgc
    25921 caagattgat tccagagatg atatctccag ccgagttgat gttttgaact gcccatgatc
    25981 cagttagttg gctttgaaca gagcgaatcg cttcgtccgt gtcttcagga gcttcggtgt
    26041 acggtgttgc atacgatcct ttttcaagtt taagacctgc aatatataga tttgtatttt
    26101 gattaagtcg ttctacacga ggaaagatga agccgtccac agtaattttg aaggtaaatg
    26161 agtaacgttt ccattcactt gttatagcga ccgatgtttc agatggaaca ccccaacctt
    26221 tctcaacaga tccatttcta tttacataga aatttactgt atcattcttc cagtcgctct
    26281 tcatccataa gctgaatgta tagacttcac caatttttgc atcaatctct tgtccaacgc
    26341 cattccacga attattttta aataggacat caacaccttt gtatttttca gcgtgctttt
    26401 tccaatttgc acccttgttt ttccaacccc ctgagaagtc ttttgtgcct ttcaacaaat
    26461 tttgaccatt gctggcattt ttagccacct caacctgaaa cagttgatta gtcagagcca
    26521 tgcgagcaac cttatccgca attccatttt cagtattgcc caaaatccgc tcgtaaagtt
    26581 tactggtttc cttaacacgc tggaagtcag tagtctctac ttttcgcgct agttgattgg
    26641 tcacattcgc aaattgacta tcagcattcg ctttgtttgc agaaacctga tcagatattc
    26701 tacccatttg tcgttcagca ttatccttgt ttgtagcgac ctgagtcttt aaatttgaaa
    26761 tctgattatc tgcgccttgt ttgttactgt ttatccgatt tgaaagattt gaaatctgag
    26821 tagtggttcc ttgctcactg cttgtaagtc tatttgatag accactgatt tgaccgccca
    26881 catcttgctt ataagtagtt atctgacttg aaatatccgt gaacttacca tctacagatt
    26941 gacgatagct agcgatttga ctagcgatgt ctttattcgc actagtttta acagcttcaa
    27001 tcctctgatt gataccctta acatcttctt gataagtagc cttaccaacg aaatcacgat
    27061 tgaccagctc acggactgct gtcgcttgtc tcgcgctctc ctcacgagta tagcgctgta
    27121 gggcttcctg tcgctgaccg tctttattta catattcctg aatagctgat aagtcggttc
    27181 gcaagccctg agctgtccgc tcaaaggtag ccttagcttc agtgatgaga ccatcagcgt
    27241 cctcaggcgc aggactccag tccgtcgcca cactaccaat ttcaaccttg attcctgtta
    27301 cccaagctgt accgcttgta gcaccttcaa gattgaatcg caatgatgtc ttcaattgat
    27361 caaaatttgt tttttcagag tagtcataag tgaatgtaat atatttccaa tctgccgaac
    27421 ctttatacat accaagcgta gcataatctg gaccactctg tactccggtc tcactatttt
    27481 ttctaaaaag ataatgtttg aagcaattaa atacattcca aaaatttcga ccttggacta
    27541 cattttcgta cttgatccaa gcgctaaaag taactttttg gtacaacctt gagctgaaat
    27601 ctggttcaag gttgaacgtt aaagtagagt tgttctctag cctatagcat tctttttgac
    27661 ctgtgacgtg gttttcaggt aatttttcaa ttacagctcc aaccgtcttg gattttatcc
    27721 atagattccg tcctcccacc ttcatttttg aaaattcttc acgcaatttc ccagcttcag
    27781 atacaactaa agtcttatct gctttatcct tggttgcgtt caggatttcc tgacggatag
    27841 agccagctcg cacctcaaat tcagcctgac tcaacttctg atttagcttg ttctgcgtgt
    27901 ctgtctcaag actcttcaca gattgcctaa tattctcagc agtcacgttg agtgcgctga
    27961 tatcggcttt agttctaaga ccttcagtca gacggcttac accagcgtcg agtgagttgg
    28021 ctcgttgttt gaaggtcgat tcaactgttg aaatctgacc ttctatatct tcaggagctt
    28081 cgctgtaatc agtcgctaac gttcctgatt cgattttcgg agcgcatatt tcgataattc
    28141 ctgcgccaga ttgtccaaat tgaattgaat tttcaattgc atcagcagta aatgtaaatg
    28201 aatatttttg ccaattttta tgagagatgg atttctgaaa tttgcgattt aaatcggtat
    28261 tatttgccca cgaacgaaat agcaagttca cgtttgcatt tgggctatcg ctagcaatcc
    28321 ttgcataaca agaaatcgtg tacttttcac caattcgcaa tctaacaagt tgagttaaat
    28381 ctttatttcc accattcgta ttttcaataa cacgaatcat gttttttatc atttttttgg
    28441 gaggatccaa aacttctacg ctaatcgctc gcccatttcc tccgcttgca ctcatccaac
    28501 atccttttga acgatcgcca atcaacaaac tcgcagtatt acgcaagagg ttgattcctc
    28561 cgacctgcac actcgctatc ttactagaga gctcctcagc tgtctgcgta agttctgact
    28621 tgctggcttt atccttggtt gcgttcagga tttcctgacg gatagagccg gctcgcacct
    28681 caaattcagc ctgactcaac ttctgattta gcttgttctg cgtgtctgtc tcaagactct
    28741 tcacagattg ccggatattt tcagcagtca cgttgagtgc gctgatatcc actttggttc
    28801 taaggccttc agtcagacga ttcacaccag cttcgagcga gttggctcgt tgtttaaagg
    28861 tcgattcaac tgttgaaatc tgactttcta tatcttcagg agcttctgaa taagaagtat
    28921 ctacatcgct tatttcaaac ttcggcatcc aaatccaaac ggttccttcc tggttgaaat
    28981 tgaacaacca ttcatttgtg gtctgcttgg attcgtttgt ccaacctttt ggaatatgga
    29041 caacatatcg tttaatttct gtcgacaatg tcacatttcc agttttatat ccgatattcc
    29101 ctaatcgaga tcttagcatt attccatttt tatttgcctt agcataaaaa ctaatggtta
    29161 catcttgatt agtcgtactt ccgggaatta ctttcccgaa ttgacccaga gctggataag
    29221 taaccttggg attccctcca tcacggccag atggattcag acctataatt ttaagagcgt
    29281 tatatccaag atacttactt tcgctatcga tagcagccgt atatgtactc gttgtccaaa
    29341 ttcctgtttt tgaaatatcc tgcttgaata gtgagttcaa gaatagattt cgaccggatg
    29401 cctgcacact cgctatctta ctagagagct cctcagctgt ctgcgtgagt tctgacttgc
    29461 tggctttatc cttggttgca ttcaggattt cctgacggat agagccagct cgcacctcaa
    29521 attcagcctg actcaacttc tgatttagct tgttctgcgt gtctgtctca aggctcttca
    29581 cagattgcct aatattttca gcagtcacat tgagtgcgct gatatccgct ttggttctaa
    29641 ggccttcagt attcgcacta gttttaacag cttcaatcct ctgattgata cccttaacat
    29701 cttcttgata agtagcctta ccaacgaaat cacgattgac cagctcacgg actgctgtcg
    29761 cttgtctcgc gctttcctca cgagtatagc gctgtagggc ttcctgtcgc tgaccgtctt
    29821 gaccaacata gctctcaact gctgccatct tagtagatag gccatcagct gtcctcttga
    29881 actcggtttt agcgactaaa agctcatttt ctccgtcttc tggtgctggt gaccagtccg
    29941 tcgccacact accgatttca accttgattc ctgttaccca agctgtaccg cttgtagcac
    30001 cttcaagatt gaatcgcaat gatgtcttca attgatcaaa atttgttttt tcagagtagt
    30061 cataagtgaa tgtaatatat ttccaatctg ccgaaccttt atacatacca agcgtagcat
    30121 aatctggacc actctgtact ccggtctcac tattttttct aaaaagataa tgtttgaagc
    30181 aattaaatac attccaagag tttcgacctt ggactacatt ttcgtacttg atccaagcgc
    30241 taaaagtaac tttttgatac aaccttgagc tgaaatctgg ttcaaggttg aacgttaaag
    30301 tagagttgtt ctctaaccta taacattctt tttgacctgt gacatggttt tcaggtaatt
    30361 tttcaattac agctccaacc gtcttggatt ttatccatag attccggcct cccaccttca
    30421 tttttgaaaa ttcttcacgc aatttcccgg cttcagatac aactaaagtc ttatctgctt
    30481 tatccttggt tgcgttcagg atttcctgac ggatagagcc agctcgcacc tcaaattcag
    30541 cctgactcaa cttctgattt agcttgttct gcgtgtctgt ctcaagcctc ttcaccgact
    30601 gcctaatatt ttcagcagtc acattgagtg cgctgatatc cgctttggtt ctaaggcctt
    30661 cagtcagacg gcttacacca gcttcgagcg agttggctcg ttgtttgaag gtcgattcta
    30721 ctgttgaaat ctgaccttct atatcttcag gagcttctga ataagaagta tctacatcgc
    30781 ttatttcaaa cttcggcatc caaatccaaa cggttccttc ttggttgaaa ttgaacaacc
    30841 attcatttgt ggtccgcttg gattcgtttg tccaaccttt tggaatatgg acagcatatc
    30901 gcttaatttc tgtcgacaat gtcacatttc cagttttata tccgatattc cctaatcgag
    30961 atcttagcat tattccattt ttatttgcct tagcataaaa actaatggtt acatcttgat
    31021 tagtcgtact tccgggaatt actttcccga attgacccag agctggataa gtaaccttgg
    31081 gattacctcc atcacggcca gatggattca gacctataat tttaagagcc ttgtgtccaa
    31141 gatacttact ttcgctatcg atagtagccg tatatgtact cgttgtccaa attcctgttt
    31201 ttggaatatc ctgcttaaat agtgagttca agaatagatt tcgaccggat gcctgcacac
    31261 tcgctatctt actagagagc tcctcagctg tctgcgtgag ttctgacttg ctggctttac
    31321 cattggccaa gttggtcagt tctgacagtc tacgagtcgt cgtctcctta tacgtcgctt
    31381 gcgctgactt cacgccagcc agttcattct tagtccggct aagtgcttca acttgcttgg
    31441 caatctcagc ttcagcctgt gcttgcttcg gtcgaatatc attcactata gtccgtttca
    31501 gagcgtccaa gtcacctgac agagccgttt gtgcgctcgt agtctgcgac ttaaacgctt
    31561 caagtctagc aacagaatcc a
//
LOCUS       DAVLSL010000006        31438 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLSL010000006 DAVLSL010000000
VERSION     DAVLSL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.006"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            55..336
                     /locus_tag="VXN30_000222"
     CDS             55..336
                     /locus_tag="VXN30_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1356902.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            468..1412
                     /gene="manA"
                     /locus_tag="VXN30_000223"
     CDS             468..1412
                     /gene="manA"
                     /locus_tag="VXN30_000223"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1356903.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1434..2909)
                     /locus_tag="VXN30_000224"
     CDS             complement(1434..2909)
                     /locus_tag="VXN30_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV1356904.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3075..3239
                     /locus_tag="VXN30_000225"
     CDS             3075..3239
                     /locus_tag="VXN30_000225"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1356905.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3240..3746
                     /locus_tag="VXN30_000226"
     CDS             3240..3746
                     /locus_tag="VXN30_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEV1356906.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(3766..4221)
                     /locus_tag="VXN30_000227"
     CDS             complement(3766..4221)
                     /locus_tag="VXN30_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1356907.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4241..5416)
                     /locus_tag="VXN30_000228"
     CDS             complement(4241..5416)
                     /locus_tag="VXN30_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1356908.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5475..6320)
                     /locus_tag="VXN30_000229"
     CDS             complement(5475..6320)
                     /locus_tag="VXN30_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1356909.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6445..7002
                     /locus_tag="VXN30_000230"
     CDS             6445..7002
                     /locus_tag="VXN30_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1356910.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7021..7488
                     /locus_tag="VXN30_000231"
     CDS             7021..7488
                     /locus_tag="VXN30_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1356911.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7577..8206
                     /gene="upp"
                     /locus_tag="VXN30_000232"
     CDS             7577..8206
                     /gene="upp"
                     /locus_tag="VXN30_000232"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1356912.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8380..8970
                     /gene="clpP"
                     /locus_tag="VXN30_000233"
     CDS             8380..8970
                     /gene="clpP"
                     /locus_tag="VXN30_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1356913.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9049..9297
                     /locus_tag="VXN30_000234"
     CDS             9049..9297
                     /locus_tag="VXN30_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1356914.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9398..10558
                     /locus_tag="VXN30_000235"
     CDS             9398..10558
                     /locus_tag="VXN30_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1356915.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QVALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10826..11695
                     /locus_tag="VXN30_000236"
     CDS             10826..11695
                     /locus_tag="VXN30_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1356916.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11699..12655
                     /locus_tag="VXN30_000237"
     CDS             11699..12655
                     /locus_tag="VXN30_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1356917.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12655..13419
                     /locus_tag="VXN30_000238"
     CDS             12655..13419
                     /locus_tag="VXN30_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356918.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13419..14129
                     /locus_tag="VXN30_000239"
     CDS             13419..14129
                     /locus_tag="VXN30_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356919.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14543..15199
                     /locus_tag="VXN30_000240"
     CDS             14543..15199
                     /locus_tag="VXN30_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1356920.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15307..16402
                     /gene="prfB"
                     /locus_tag="VXN30_000241"
     CDS             join(15307..15378,15380..16402)
                     /gene="prfB"
                     /locus_tag="VXN30_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1356921.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16420..17112
                     /gene="ftsE"
                     /locus_tag="VXN30_000242"
     CDS             16420..17112
                     /gene="ftsE"
                     /locus_tag="VXN30_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1356922.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17105..18031
                     /locus_tag="VXN30_000243"
     CDS             17105..18031
                     /locus_tag="VXN30_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1356923.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVETNTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            18317..20497
                     /locus_tag="VXN30_000244"
     CDS             18317..20497
                     /locus_tag="VXN30_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1356924.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20549..21364
                     /locus_tag="VXN30_000245"
     CDS             20549..21364
                     /locus_tag="VXN30_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1356925.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21504..22847
                     /locus_tag="VXN30_000246"
     CDS             21504..22847
                     /locus_tag="VXN30_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1356926.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23062..24252
                     /locus_tag="VXN30_000247"
     CDS             23062..24252
                     /locus_tag="VXN30_000247"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1356927.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            24805..25740
                     /locus_tag="VXN30_000248"
     CDS             24805..25740
                     /locus_tag="VXN30_000248"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1356928.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            25774..26811
                     /locus_tag="VXN30_000249"
     CDS             25774..26811
                     /locus_tag="VXN30_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1356929.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            26952..27557
                     /locus_tag="VXN30_000250"
     CDS             26952..27557
                     /locus_tag="VXN30_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1356930.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            27715..28245
                     /locus_tag="VXN30_000251"
     CDS             27715..28245
                     /locus_tag="VXN30_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1356931.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            28266..29168
                     /locus_tag="VXN30_000252"
     CDS             28266..29168
                     /locus_tag="VXN30_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEV1356932.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            29168..29650
                     /locus_tag="VXN30_000253"
     CDS             29168..29650
                     /locus_tag="VXN30_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1356933.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            29652..31193
                     /locus_tag="VXN30_000254"
     CDS             29652..31193
                     /locus_tag="VXN30_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1356934.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(31248..>31438)
                     /locus_tag="VXN30_000255"
     CDS             complement(31248..>31438)
                     /locus_tag="VXN30_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1356935.1"
                     /translation="KEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRI
                     FIALNIKKERTNFVLSRA"
BASE COUNT         9565 a         5864 c         6882 g         9127 t
ORIGIN      
        1 tataatgctt tttgatttta gtatagtatt atcgtagaaa atggactcgg tgctatggat
       61 acaccggatg agaatggtta tgtagcagat gactatcgga ttacttactt agaggcccac
      121 atcaaggcca tgcgagatgc catttacaaa gacggggttg acttgcttgg ttatacgaca
      181 tggggctgta tcgattcggt ttcagctggc acaggggaaa tgaataagcg ctatggcttt
      241 atctatgtag atcgagataa tgtaggaaat gggactctca aacgtagcaa gaagaagtcc
      301 ttctattggt acatgtcatt tatagcaatg gtgtaagcat cgagtagagc acatttgttt
      361 actattttta taaaatcttc tccctgcttt ataagatgta aaaaagagtt caattcgaac
      421 tctttttttg ctataatgag gggagaaaaa tcagacagga gattaagatg tcagaaccat
      481 tatttttaca atcagttatg caagaaaaaa tctggggtgg taccaagcta cgtgatgagt
      541 ttggctacga catcccaagt gaaaaagtcg gagagtactg ggctatctca gctcatctaa
      601 atggtgtttc taaagttgcc aatggtcgtt acgagggaac agatcttgct actttgtatg
      661 cggaacaccg tgaattattt ggcaatcgtc ctgagcctgt atttccgctt ttgactaaga
      721 ttttggatgc taacgactgg ctcagcgttc aagttcaccc agacgatgct tatggactag
      781 agcatgaagg cgaacttgga aaaacagaat gctggtacat catcgctgcg gatgaaggtt
      841 cagagattat ctacggccac aatgccaagt caaaagaaga actccgccag caaatcgagg
      901 ataagaactg ggatgcgctg ttgacaaaag tgccagttaa agcaggagat ttcttctatg
      961 taccaagcgg taccatgcac gctatcgggg cgggtatctt gattcttgaa acccagcagt
     1021 ctagcgatac cacttaccgt gtctatgact ttgaccgtaa ggatgacaag ggcaatttac
     1081 gtgaacttca ccttgaaaaa tctatcgatg tcttgaacat tggtgaaccc gcaaatagcc
     1141 gtccagtgac aatcaaagca gatgatctac gttccactct ccttgtatcc aatgatttct
     1201 tcgcagttta caaatgggaa attactggaa aagttaactt tgaaaagaca gctgactaca
     1261 gcttgttgag tgtcttggct ggtcaaggtc aactaactgt tgacgggaaa aactatccaa
     1321 tccaaaaagg gagacacttt atcctaccaa gtgatgttga agcttggact ctggaagggc
     1381 aagatttaga attaattgtt agtcatccat aaaaaagaaa gggcctgagt tcattactca
     1441 agtccttttt gattacataa attgggcgat gaagaccacg atgatgattg gaatgacgga
     1501 acgaagaaac gaagaaggaa gagccagact tggaacagtc cttgtttcca tgctctttca
     1561 tcgagatgga gttcctccat tgcaagagcc tttttaaaga tatagcctgt aaaaagtgaa
     1621 aggtagagag ctccaaatgg catgaggaga ttgggaacca agaagttcat agcgtcaaag
     1681 aaggtcttac caaaaatgtg aacatccgcc atgacaccgt aagatagggc tgaaggaatg
     1741 ccaaagacaa aggtcaaaat tcctaaaata acactccatt tggcacgttt gctgttatcc
     1801 tggttggtga tattgcccac attgatctcc agcatcacga cagaagaagt gacagtcgca
     1861 aagaggaaga ggacgtagaa aatggttcca aaaggcatct tgtcaaagag ttgaggcaag
     1921 acgataaaga gcaagctcgg tcccccttca gactggatat tgaaggggga ccgagcttgg
     1981 aaaatggcta gacctgccat gatggatatc gagatattca tggctacgat ggagattcct
     2041 gactggacta gattggtttt cttgtccaag taagaagcat aggtcaacat gactgtaacc
     2101 cctagtgaga gggcaaagaa agattgtccc agagcataga ggagaccagc gctagtcagt
     2161 tttgaaaagt ctggtttgag gaagtaaaga accccttcca tggcatttgg caaactgaga
     2221 gagcgaccga tgataacaac aaagatgata aagagcaggg gcatcatgac tttcgaagct
     2281 ctttcaatcc ctttttgaac cccacgtgat acaataaaga tatttaataa gataaaggct
     2341 gcttgagctc ctagggcaat ggctggattt gaaatgattg aagtaaataa ctgagcataa
     2401 tcacccgttc caccaagttg gaacaatttc ccaaactcaa tacctagata gactagaatc
     2461 catcctccga taacactgta aaaagataag aggataaaga gggcaaaggc gccaatccaa
     2521 ccgataaagt tgtacttgtt attcttgccc agttttccaa aggttttgat agcggaaacg
     2581 ccagcactac ggccaagggc aaactcagcc agcaggagag ggaaaccgat taaaatagtg
     2641 gaaatgagaa agattagtaa aaagcctcca ccgccattag cagcagtcat gtagggaaac
     2701 ttccaaacgg aaccaagccc gatgaccgag ccagcagatg ctagaataaa gccaagtttc
     2761 gacccccatt gtgatttttc tgacatagtt aaactctgat ctcctttttc aaaataagaa
     2821 aaagctactt gagttgtcaa atagccttct gtttgattga tagacttgac tatcctatta
     2881 tgttttctaa ggtctgtcaa gaaaaccatt ttcaagtttt cacacctttc tcaaaaacgc
     2941 ttgactctga cctaagggga ggggttatac tatcattgta aggaggaaat catgtaccat
     3001 ataaaagaag ctgcgcagct ttcaggtgtc tctgtcaaga ccctgccctg catcactatg
     3061 acaagatagg actcttggtc cccttaaagt cggaaaacgg ctatcgaacc tatagtcaag
     3121 aggatttgga acgccttcag gtcattcttt actacaaata tctaggcttt tctttagaga
     3181 aaatagcaga actgttaaag gaagaaagga cagatttatt gctccatttg actaggtagt
     3241 tggactatct aactcgcgaa aggcaacatc tggatacctt gatttccacc ttgcaaaaaa
     3301 ctattcaaga acaaaaagga gaaagaaaaa tgaccattga ggaaaaattc acgggattta
     3361 gctatcaaga caatcaaaaa taccaccaag aagcggtaga gaaatatggt caagaagtca
     3421 tgggacaagc gctcgaacgc caaaaaggtc acgaagacga ggctacggcc gcctttaacc
     3481 aagtctttca aactttggca caaaatcttc aagttggttt acctgcaaca gcaaccgaaa
     3541 accaggagca agcagccaag ctcttgcaag ccattcgcac ttatggattt gactgctcta
     3601 ttgaggtatt cggtcatatc ggtaaaggtt acgtctacaa cccagagttt aaggaaaaca
     3661 ttgacaagtt tggttctgaa acagcccagt acacgtcaga tgccattgcg gcttacgttc
     3721 agacaaatgc agaaaaaata ggctaggaat ttcctagcct attttttact tcaaatcata
     3781 aagccagtcg tcaccgtttt tgtagtaaaa gaattcactg agatcttctt ctagaaacac
     3841 acgaagcata tcagacatat catcggttgc aagttttaga tgagaaagat tttcaaagtc
     3901 ctcccaccaa actttccctt cgtctgaaga ctggagttca ccagtaaagt gttctgtctt
     3961 gtaaaaaagg acgacataac gataatcctt gtcgtcatac cagtttttga taccacagag
     4021 ttggggtttg gaaatgatca gaccagtttc ttctttcact tcacgaatga cagcatcgac
     4081 aaaggattcg ccacgttcaa catgaccacc aggaaaagta atgccagacc agtcgggact
     4141 aactcggtct tggaccagga ccttatctcc gtttttaatc atacacatgt taacaaattc
     4201 gactgcctct cttctgttca ttcttcacaa cctttaatct ttaatcataa tgcagacttc
     4261 ccgccaccca gccggtacag agggcagaag tgatgttaaa gccacccgtg tgggcattga
     4321 tatccataac ttcgcctgca aagtggaggc caggtaccag cttactttca agggttttag
     4381 gattgatttc cttgagactg actccaccct tggtaacaaa ggactttgca agggacattt
     4441 ttccagttac aggaatttta agttctttaa tggactggac aagttgttct cgttcctttt
     4501 cagtcagttg tttgactttt tcaggatatc cttgtacaaa aaattcggcc aagcgttctg
     4561 gtaacaaggt ttttaaagcg tttttcaagg atttttcccg attttcttct agaaatgtaa
     4621 ccaagtcctt ctcagaaagt tgaggcaaaa catcgagtga gagaacctcc ccacctttga
     4681 caaagctaga catgcgtagg gcagcaggac ctgacaaacc aaagtgggta aagagtaaat
     4741 catgagtgat gacatgctta ccataactta gggtcacatc gtccagagaa atcccttgta
     4801 aggctttatg tggaaaatct gttaataaag gactttcagc agcctcaaga tcggtgatgg
     4861 tatgcttaaa atggcgagca atctcgtgac caaaaccagt cgaaccagtc gaaggataag
     4921 acttaccacc tgttgtgaca atgagtttct cacaagtgaa ggtttgatcc gctgacttaa
     4981 ggacaaactg gtcatctact tttttaacag aaacgatttc tatttgagta gcaacttgac
     5041 cacctagttc ggtgattttc ttttccaaag cttcgataat agtccgagac ttgtcactgg
     5101 ctggaaagac gcgtccgtgg tcttcgacct taagtttaac accattttct gtaaaaaagt
     5161 tgatgatgtc atgattatcg aactgggaga aaacactgta aagaaagcgt ccgtttccag
     5221 gaattccagc tagcaggttg tctaagctac cattgttggt cacattgcaa cgtcccccac
     5281 cagtcccagc taattttttt ccaagtttcc gatttttttc gatgaggagg gttttctgtc
     5341 cataaaagct actggaaatc gtagccatca taccagcagg tcccccaccg atgacaatag
     5401 tatcaaaatg tttcatagct ctattgtacc acaaaaaaac aagagatgat ggtcacctct
     5461 tgtcaagaat gcaattaatc aatttcatag cccatcagca aaccgccctc ttctgcatag
     5521 aaactgcaga gaccagaggt tggtagaatt ttaatatccg cttgtgggaa ggtttcacgg
     5581 attcgctctg agagctgttg acaacatttt tcgttattgc gttgggccat gacaatacgg
     5641 ccaccagcat atccagcttt tactaactca tcataggcag cttgaactga tttctttgat
     5701 ccccttgctt tttgtagcaa ttcgagagtt ccagtttcac tagcttttcc gaccatacga
     5761 atgttgagaa ggccaacgac cgtaccgata agcttgctca aacggccgtt cttcaccaag
     5821 ttatcgactt tggctaggac aaagagcaac ttagtttttt cttgataggc agtgatagct
     5881 tcaaccactt cttcaaaaga caagccctgg tcaatcaagt cattcaattt ttctacgagt
     5941 aggtcaactt caccaccagc agataaacta tcaatcacat gaatcttagt gtcaggatgg
     6001 tcttctagat aaatattctt tgctagttga gcactattgt gactgccaga aagggtacct
     6061 gtgatggtta ctaggaaaat gtttttggca ccttcaaatg ctcgcaaata gtcatctggg
     6121 cttggacaag ccgattttga agcttctgca gttgcataca tggtttccat catttggtca
     6181 atatcgagac tggcgtcatc aacaaagacc tgatcagcta cttgaatggt taaggggaca
     6241 cttacaaagg ttgtgttaat agctggtgtt ggcagttgac gataatcaca accagagtca
     6301 gcaataattt tccaagtcat agaaattctc catctttgtc agttatacat tgacaaaggt
     6361 tctgtctttt tttacaatta tatcatgaaa acccttgaaa caaaagcatc atccgtctgt
     6421 tgtgacaagt agaaagaaaa tgttatgtct gaacgtagaa tctctgaaaa gtctcttgaa
     6481 aatctcagaa aatcaaacca agaatccaat ttattaacca gagaagccat tgaaacagcc
     6541 ctcttgcaac tcttggaaaa aaaggagctg accaagatta gtatttctga attggtcaaa
     6601 cgtgcaggtg tttcgcgtgc ggccttttat cgcaattatg actccaaaga ggaaatttta
     6661 gagagcgtct ttaaacgaac tgtccacaat attatggaac agctgcatca ttacgattta
     6721 aagacagacc tttatttggt ttgggttcac cttttccggg aggccagaaa ggaagctaga
     6781 gtgattcaat tggccttgga ttaccatctg gaaaaaatct ttgtccaagc catgcaggaa
     6841 tttctagaaa aataccatgg gaaatcaaaa ggtgtcagct cttatcttca ttccttctgg
     6901 agctcagcca tcgtctctgt ccttctaaaa tggatcaagg atggcatgaa ggtaccagct
     6961 gaaaagattg cagatttagg tttaccattt tttaaaaaat agagaaaaag gagaaaagag
     7021 atgactgaaa aaagactggc ctgggatgag tattttgcag cccaagccct actaattgcg
     7081 aatcgttcca cttgtaaacg tgctaaagtg ggcgcgattc tggtaaaaga taataaggtt
     7141 atttccactg gttacaatgg ttcggtgtca gggactgagc attgtattga tcacgaatgt
     7201 ttggtcattg aaggccactg tgttcgcacc cttcacgctg aggtcaatgc tatccttcaa
     7261 ggtgctgagc gtggtgttcc caaaggcttt acagcctatg taacccattt tccttgtctc
     7321 aactgtacaa aacaattgct tcaggtcggc tgcaagcgcg tggtttatat caaccagtac
     7381 cgaatggacg actacgccca atacctttat caagaaaaag ggacagaatt gacccattta
     7441 ccacttgaga cagtacagac agctcttaaa gaggctgatc taatgtaaaa attatcaaaa
     7501 ataaatggct tagaaagatt tttaaaccgt tttttggtat aataagaaga ataaattgaa
     7561 agaaggaatt ccaaaaatgg gaaaaattga agttattaat cacccactga ttcaacacaa
     7621 attgtcaatc ttgcgtcgta cagatacttc tacaaaagct tttcgtgagc tagtagatga
     7681 gattgcaatg ttgatggggt atgaagtact tcgtgatctt ccactagaag atgtggaaat
     7741 cgaaacacca attacaaaaa cagttcaaaa acaattggca ggtaagaaat tggccatcgt
     7801 cccaatcttg cgtgcaggta tcgggatggt tgatggtctc ttgagcttgg ttccagctgc
     7861 taaagttggc cacatcggta tgtaccgtga tgaagaaaca cttcaaccag ttgaatactt
     7921 ggtgaaattg cctgaggaca ttgaccaacg tcaaattttt atagtagacc caatgttggc
     7981 aacaggtggc tcagcaatct tggctgttga ttctcttaaa aaacgtggcg catcaaatat
     8041 caaatttgtc tgccttgtat ctgctccaga gggtgtaaaa gcccttcaag aagctcatcc
     8101 agatgtagaa atctttacag cagccttgga tgaacgtttg aacgaacacg gttatatcgt
     8161 tccaggtctt ggagatgctg gagaccgctt gttcggtaca aaataagatc gaaaagaaga
     8221 atgacttgga aaagtatttc cagtcacgaa aggaggttgg gtttttgttt ctgtctaatg
     8281 aaagcagagc aaaaatttga ccttttttga ccaagatatt ataatagtct tatcttcagt
     8341 catttgacca acaaaattaa aactcaaaag gagaaatgaa tgattcctgt agttattgaa
     8401 caaacaagcc gtggagaacg ttcttacgat atttactcac gtcttctcaa agaccgcatc
     8461 attatgctga caggtccggt tgaagacaat atggctaact ctgttattgc ccaattgctt
     8521 ttcttggatg cccaagatag tacaaaagat atttaccttt atgtcaatac accaggtggt
     8581 tctgtttcag ctggtttggc aatcgtagat accatgaact ttatcaaggc agatgtccaa
     8641 accattgtta tgggaatggc tgcatctatg ggaacagtca tcgcatcaag tggagcaaaa
     8701 ggcaaacgtt tcatgcttcc aaatgctgaa tacatgattc accaaccaat gggcggtaca
     8761 ggtggtggta cccaacaaac tgatatggct atcgctgcag aacacttgct caaaactcgt
     8821 aataccttgg aaaaaatctt ggctgaaaat tcaggtcagt caatggaaaa agtccatgca
     8881 gatgcagaac gtgataactg gatgagcgcc caggaaacac ttgaatatgg ctttattgat
     8941 gaaattatgg ccaacaattc attgaactaa tgatgataga aggcaaactc gactgggttt
     9001 gctttttttg gtataatagg gagagatttc ttagaaagag gatttatcat gtttgaaaaa
     9061 gtcaatcgct ctggcttgat tatctatctt tactataatc gtgatgccaa aaaactgcag
     9121 gattatggag atattaccta tcattccaag aaacatcgtt acttacaact ctatgttcca
     9181 actcaagaag tggagcaatt ggtcggacgc ttgagcaagg aaaaatttat taaaaaagtt
     9241 agggtttgtc atatccaaga gttggaaaca ccctttgtgg gcaatcttta tcgataggaa
     9301 aacgttatca tcgaaaaagt tcaaggaaaa cattgacaat tttctgataa ttcggtatat
     9361 tcttaacaga ctatttaaga aataaggaga caaaaagatg aagaaaaaat ttgccctatc
     9421 gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt ggagaagtga agtctggagc
     9481 agtcaacact gctggtaact cagtagagga aaagacaatt aaaatcgggt ttaactttga
     9541 agaatcaggt tctttagctg catacggaac agctgaacaa aaaggtgccc aattggctgt
     9601 tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa atcgaagtag tcgataaaga
     9661 taataagtct gaaacagctg aggctgcttc agttacaact aaccttgtaa cccaatctaa
     9721 agtatcagca gtcgtaggac ctgcaacatc tggtgcgact gcagctgcgg tagcgaacgc
     9781 tacaaaagca ggtgttccct tgatctcacc aagtgcgact caagatggat tgactaaagg
     9841 tcaagattac ctctttattg gaactttcca agatagcttc caaggaaaaa ttatctcaaa
     9901 ctatgtttct gaaaaattaa atgctaagaa agttgttctt tacactgaca atgccagtga
     9961 ctatgctaaa gggattgcca aatctttccg cgagtcatac aagggtgaaa tcgttgcaga
    10021 tgaaactttc gtagcaggtg acacagactt ccaagtagcc cttacaaaaa tgaaagggaa
    10081 agactttgat gctatcgttg ttcctggtta ctataatgag gctggtaaga ttgtaaacca
    10141 agcgcgtggt atgggaattg acaaaccaat cgttggtggt gatggattca acggtgagga
    10201 gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc tactttatct caggcttctc
    10261 aactactgta gaagtttcag ctaaagctaa agccttcctt gacgcttacc gtgctaagta
    10321 caatgaagag ccttcaacat ttgcagcctt ggcttatgat tcagttcacc ttgtagcaaa
    10381 cgcagcaaaa ggtgctaaaa attcaggtga aatcaaggat aaccttgcta aaacaaaaga
    10441 ttttgaaggt gtaactggtc aaacaagctt cgatgcagac cacaacacag tcaaaactgc
    10501 ttacatgatg accatgaaca atggtaaagt tgaagcagca gaagttgtaa aaccataata
    10561 gaaaaatgtt gaaataggga atgagccttt gactcactcc ctgtttcgat atttaatact
    10621 cttcgaaaat ctcttcaaac tgcgtcaacg tcgccttgga ttatatatgt gactgacttc
    10681 gtcagtctta tctacaacct caaagcagtg ctttgagcaa cctgcggcta gtttcctagt
    10741 ttgctctttg attttcattg agtataagaa cctatcaaaa agtgagggaa acccctcgga
    10801 attataaata gaaagagtga atcttatgct ccaacaactc gtaaatggtt tgattctagg
    10861 tagtgtttac gcgctgttag ccctaggata taccatggtt tacggaatta tcaagctcat
    10921 caacttcgcc catggtgata tttatatgat gggagccttt atcggttatt tcttgatcaa
    10981 ttctttccaa atgaatttct ttgtagcgct tattgtagct atgctagcga cagctattct
    11041 tggtgtcgtg attgagtttc ttgcttaccg acctttgcgc cactctactc gtattgctgt
    11101 tttgattacg gctattgggg tttctttcct attggagtat ggaatggtct atctggttgg
    11161 tgccaatacc cgtgccttcc ctcaagcgat tcaaacagtt cgatatgatt tgggaccaat
    11221 tagcttaaca aatgtgcagt taatgatttt ggctatttcc ttgattttga tgattttgtt
    11281 acaagtcatt gtccaaaaga ctaagatggg gaaagccatg cgtgcagtat cagtagatag
    11341 cgacgcggcg caattgatgg ggatcaatgt aaaccgtacg attagcttta ccttcgcttt
    11401 gggttctgct cttgcgggtg cggctggtgt tctgattgct ctttattata actctcttga
    11461 gcctttgatg ggggttactc caggtcttaa atctttcgtt gccgcagtac ttggtggtat
    11521 cggaattatt cctggtgcgg ctcttggtgg ctttgtgatt ggtctattgg aaacctttgc
    11581 gactgccttt gggatgtcag atttccgtga tgccattgtt tatggaatct tgttgttgat
    11641 cttgattgtc cgcccagctg gtatccttgg taagaatgtg aaagagaagg tgtaaacgat
    11701 gaaggaaaat ttaaaagtta atattctatg gttactcctt ttgttagctg gctatagctt
    11761 gattagtgta ctggtttcag tcggagtact taatctattc tatgtacaga ttttacagca
    11821 aattggaatt aatattattt tggctgttgg tctcaactta atcgttggtt tttcaggaca
    11881 attttcactt ggtcatgctg gtttcatggc gattggtgcc tatgcagcag ctattattgg
    11941 ttctaaatta ccaacctacg gtgccttctt tggagctatg cttgtagggg ctttgctttc
    12001 aggagcagtt gccttacttg tcggcattcc aaccttgcgc ttgaaggggg actatcttgc
    12061 ggtagcaact ctgggtgttt ctgaaattat ccgtatcttt atcatcaatg gtggaagcct
    12121 tacaaatggt gcggcaggta tcttagggat tcctaacttt acaacttggc aaatggttta
    12181 cttctttgtc gtgattacaa ccattgcaac cttgaacttc ttgcgtagcc caattggtcg
    12241 ttcaaccctc tctgttcgtg aagatgaaat cgctgctgag tcagttgggg ttaatacgac
    12301 taaaattaaa atcatcgctt ttgtctttgg tgccattact gcaagtattg ctgggtcact
    12361 tcaggcagga tttatcgggt ctgttgtacc gaaagattac accttcatca actcaatcaa
    12421 cgttttgatt attgttgtat ttggtggact cggttccatt acaggtgcga ttgtttcggc
    12481 tattgttctg ggaattttga atatgcttct ccaagatgtt gctagtgtgc gtatgattat
    12541 ttacgctttg gccttggtat tggtaatgat tttcagacca ggtggactcc ttggaacatg
    12601 ggaactgagc ctatcacgtt tctttaaaaa atctaagaag gaggaacaaa actaatggca
    12661 ttacttgaag taaaacagtt aaccaaacat tttggtggtc taacagctgt tggagatgtg
    12721 actcttgaat tgaacgaagg ggaactggtt ggattaatcg gtccaaacgg agctgggaaa
    12781 accacccttt tcaacctttt gaccggtgtt tatgaaccaa gcgagggaac agtaacccta
    12841 gatggtcacc ttttgaatgg gaaatcacct tataagattg cctctttggg acttggacgt
    12901 actttccaaa atatccgtct ctttaaagat ttaacagttt tagacaatgt tttgattgct
    12961 tttggaaacc atcacaaaca acatgttttt actagtttct tacgcttacc agctttttac
    13021 aagagtgaaa aagaattaaa ggctaaagct ttggaattgt tgaaaatctt tgatttagat
    13081 ggtgatgcag agactcttgc taaaaatctt tcctacggac aacaacgtcg tttggaaatt
    13141 gttcgtgccc ttgctacgga acctaaaatt ctcttcttag atgaaccagc agcaggtatg
    13201 aacccacagg aaacagccga attgactgag ttaattcgtc gtatcaaaga tgaatttaag
    13261 attacaatca tgttgattga acacgatatg aatctggtca tggaagtaac agaacgtatc
    13321 tacgtacttg aatatggccg tttaattgct caaggaactc cagacgaaat taagaccaat
    13381 aaacgcgtta tcgaagctta tctaggaggt gaagcctaat gtctatgtta aaagttgaaa
    13441 atctttctgt gcattacggt atgatccaag cagtccgtga tgtaagcttt gaagttaatg
    13501 aaggagaagt tgtttccctt atcggtgcca acggtgcagg taagacaact attcttcgca
    13561 cattgtcagg tttggttcga ccaagttcag gaaagattga atttttaggt caagaaatcc
    13621 aaaaaatgcc agctcagaaa attgtggcag gtggtctttc acaagttcca gaaggacgcc
    13681 acgtctttcc tggcttgact gttatggaaa atcttgaaat gggagctttc ttaaagaaaa
    13741 atcgtgaaga aaatcaagct aacttgaaga aggttttctc acgctttcct cgtcttgaag
    13801 aacgtaagaa ccaagatgca gctactcttt caggagggga acaacaaatg cttgccatgg
    13861 gacgcgctct tatgtcaaca ccaaaacttc ttcttttaga tgaaccatca atgggacttg
    13921 ccccaatctt catccaagag atttttgata tcattcaaga tattcagaag caaggaacaa
    13981 ccgtcctctt gattgaacaa aatgccaata aagcacttgc aatctctgac cgaggatatg
    14041 tactggaaac agggaaaatc gtcctatcag gaacaggaaa agaactcgcc tcatcagaag
    14101 aagtcagaaa agcatatcta ggtggctaaa acaatccagt ggattgtttt agtcggcaga
    14161 tgaagattac gaagtaatca tcattatagt ctgggagact agtttaggtt gcagataaag
    14221 attgcgaagc aatcatcaaa tccagtggat tgttttagtc ggcagatgga gattacgaag
    14281 taatcatcaa tatagtccgg gggacctttt tagtcggtag attgagattg caaacaaatc
    14341 tgcatctaca ttgaaagctt aatttctaat aattgaaaaa atcgaatgaa aaatttctta
    14401 ccttcattca cagagctcga tttcagagct ctttttgcta gcttattcat acttttctga
    14461 atttcgaaaa agaaatgtaa gcgtttgata gatttacaaa aagattgtat aatagggata
    14521 agaatagaaa aggagaagtc tcatggcagt taaagatttt atgacccgca aggtagttta
    14581 tattagtcca gatacaacag tatctcatgc agcagatttg atgagagagc aaggtttgca
    14641 ccgtctgcct gttatcgaaa atgatcaatt agttggtttg gtgactgagg gaaccattgc
    14701 acaagcaagt ccatctaaag caacaagtct ttctatctat gagatgaatt atcttctgaa
    14761 taagacaaaa gtaaaagatg tcatgattcg cgatgttgtc actgtctcag gctatgctag
    14821 tctagaagat gcaacttatc tgatgttgaa aaataagatt agtattctcc ctgtcgtaga
    14881 taaccatcaa gtatacggag ttattactga ccgtgacgtt ttccaagcct ttcttgaaat
    14941 tgcaggttat ggcgaagaag ggattcgtgt acgctttgtt acagaagatg aagttggtgt
    15001 tcttggaaaa attgtttctt tgattgtaga agaaaatttg aatatctccc atacagtcaa
    15061 tattccgcgt aaggatggta aggtgattat cgaagtgcaa atcgatggat caattgattt
    15121 accagccttg aaagaaaaat ttgaagcaaa tggtattcaa gtggaagaaa tcgctcgcac
    15181 ttcagcaaaa gtcttgtaag aagggaagcc caaaggcttc ttttttcatg aaaaggggat
    15241 tagagaaaaa gatggaaaga aatgataaaa tatgctataa tgaaataatg taaaaaagga
    15301 gtatttatgg acatttcagt aattcgtcag aaaattgacg caaatcgtga aaaattagct
    15361 tctttcaggg ggtctctttg acctcgaagg gctagaggaa gagattgcca tcttggaaaa
    15421 caagatgaca gaacctgatt tttggaacga taatattgca gcccaaaaaa cgtcgcaaga
    15481 attaaatgaa ttaaaaaaca cttacaatac cttccataag atggaagagt tgcaggatga
    15541 agtcgaaatt ttattggatt ttttggctga agacgagtca gtgcatgatg aactggtagc
    15601 gcagttagcc gaacttgata agataatgac cagctacgag atgactctac tcttgtcaga
    15661 accttatgac cacaacaatg ccatcttgga aatccatcca ggttctggtg gtactgaggc
    15721 gcaggactgg ggtgatatgt tgcttcgtat gtatactcgt tatggtaatg ctaaaggctt
    15781 taaagtggaa gtgttggatt accaagcagg tgatgaggct ggtattaagt cggtaacttt
    15841 atcatttgaa gggcctaatg cctatggtct cctcaagtca gaaatgggtg ttcaccgctt
    15901 agtgcgaatc tcaccatttg actctgccaa acgtcgccat acctctttca catctgtaga
    15961 agtgatgcca gaattggatg atactattga agtggaaatc cgtgaagatg atatcaagat
    16021 ggataccttc cgttcaggtg gtgccggtgg acaaaacgtc aataaggttt caacaggtgt
    16081 acgtttaacc cacattccaa ctggaattgt tgtccaatca acagtggatc gtacccagta
    16141 tggaaataga gatcgtgcca tgaagatgtt gcaggctaag ctctatcaaa tggagcaaga
    16201 gaagaaggct gcagaggtag attctctcaa aggtgagaaa aaggagatca cttggggaag
    16261 ccaaatccgt tcttatgtct tcacgcctta tactatggta aaagatcacc gaactagctt
    16321 tgaggttgct caggtagata aggttatgga tggggactta gatggtttta tcgatgctta
    16381 tctcaagtgg cgaattagct aagatagaaa ggaactcaca tgtcaattat tgaaatgaga
    16441 gatgtcgtta aaaaatacga caacggaacg actgctctac gcggtgtttc ggttagcgtt
    16501 caaccggggg aatttgctta catcgtagga ccttcaggag cagggaagtc aacttttatt
    16561 cgttctctgt atcgtgaagt aaaaatcgat aaaggaagcc tatcagttgc tggttttaat
    16621 ctggttaaga tcaaaaagaa agatatcccg cttctacgtc gtagtgttgg ggttgtcttc
    16681 caggattata aattgttacc aaagaaaact gtctatgaaa atattgctta cgctatggaa
    16741 gtaatcgggg aaaatcgccg taatatcaaa agacgagtga tggaagtttt ggacttggtt
    16801 ggattgaagc ataaggttcg ttctttccca aatgaactct caggtgggga gcaacagcgg
    16861 attgcgattg cgcgtgcaat tgtaaataat cccaaagtat tgatagctga tgagccaaca
    16921 ggaaatctgg atccggataa ttcatgggaa attatgaatc ttttggaacg gattaaccta
    16981 caaggaacaa ctattttgat ggcgactcat aatagccaga ttgtaaatac cttgcgccac
    17041 cgtgtcattg ccattgaaaa tggccgtgtc gttcgtgacg aatcaaaagg agagtatgga
    17101 tacgatgatt agtagatttt ttcgccattt atttgaagcc ttaaaaagtt tgaaacgaaa
    17161 tggttggatg acagtagctg ctgtcagttc agtcatgatt actttgacct tggtggcaat
    17221 atttgcatct gttattttca atacagcgaa actagctaca gatattgaaa ataatgtccg
    17281 tgtagtagtt tatatccgaa aggatgtgga agataatagt cagacaattg aaaaagaagg
    17341 tcaaactgtt acaaataatg actaccacaa ggtatatgat tctttgaaga acatgtctac
    17401 ggttaaaagt gttacctttt caagtaaaga agaacaatat gaaaaattaa ccgagataat
    17461 gggagataac tggaaaatct ttgaaggaga tgccaatcct ctctatgatg cctatattgt
    17521 agagacaaac actccaaatg atgtaaaaac tatagccgaa gatgctaaaa aaattgaagg
    17581 tgtctctgag gttcaagatg gcggtgccaa tacagaaaga ctcttcaagt tagcttcatt
    17641 tatccgtgtt tggggactag ggattgctgc tttgttaatt tttatcgcag ttttcttgat
    17701 ttcaaatacc attcgtatta ccattatttc ccgcagtcgc gaaattcaaa tcatgcgctt
    17761 ggtcggagct aaaaacagtt atatccgtgg accgttcttg ttagaaggag cctttatcgg
    17821 tttattggga gctatcgcac catctgtttt ggtctttatt gtttatcaaa ttgtttacca
    17881 atctgtcaac aaatcgttgg tagggcaaaa tctatccatg attagtccag atttatttag
    17941 tccgttgatg attgccctac tatttgtgat tggggttttc attggttcat tgggatcagg
    18001 aatatccatg cgccgattct tgaaggttta ggtaaaatag ctgcttttat gaggagattg
    18061 taaaatctcc ttttttgcta caagagtttt tgaaaagaga tgcgcagaag aaaagagctt
    18121 ccaaagaagt cccccagaga agacttcttg atggctaatt tgaacttgaa atttggcgca
    18181 ctagaaattt tttagaaaaa agtgtaattt ttttataaaa gcctttacaa aaaaaattaa
    18241 agtggtataa ttaaatcata gaaagtgcaa acgttttcgt aaaacgtttg cctaaataaa
    18301 aataaaggag atttgaatga tgaaagatac attcaaaaat gtcttgtctt tcgaattttg
    18361 gcaaaaattc ggtaaggctt tgatggtagt tatcgcggtt atgccggctg ctggtttgat
    18421 gatttcaatc ggtaagtcta tcgtgatgat taacccaacc tttgcaccac ttgtcatcac
    18481 aggtggaatt cttgagcaaa tcggttgggg ggttatcggt aaccttcaca ttttgtttgc
    18541 cctagccatt ggaggaagct gggctaaaga acgtgctggt ggtgctttcg ccgctggtct
    18601 tgctttcatc ttgattaacc gtatcactgg tacaatcttt ggtgtatcag gcgatatgtt
    18661 gaaaaatcca gatgctatgg taactacttt ctttggtggt tcaatcaaag ttgctgatta
    18721 ctttatcagt gttcttgaag ctccagcctt gaacatgggg gtattcgtag ggattatctc
    18781 aggttttgta ggggcaactg cttacaacaa atactacaac ttccgtaaac ttcctgatgc
    18841 actttcattc ttcaacggga aacgtttcgt accatttgta gttattcttc gttcagcaat
    18901 cgctgcaatt ctacttgctg ctttctggcc agtagttcaa acaggtatca ataacttcgg
    18961 tatctggatt gccaactcac aagaaactgc tccaattctt gcaccattct tgtatggtac
    19021 tttggaacgt ttgctcttgc catttggtct tcaccacatg ttgactatcc caatgaacta
    19081 cacagctctt ggtggtactt atgacatttt aactggtgca gctaaaggta ctcaagtatt
    19141 cggtcaagac ccactatggc ttgcatgggt aacagacctt gtaaacctta aaggtactga
    19201 tgctagtcaa tatcaacact tgttagatac agtacatcca gctcgtttta aagttggaca
    19261 aatgatcggt tcattcggta tcttgatggg tgtgattgtt gctatctacc gtaatgttga
    19321 tgctgacaag aaacataaat acaaaggtat gatgattgca acagctcttg caacattctt
    19381 gacaggggtt actgaaccaa tcgaatacat gttcatgttc atcgcaacac ctatgtatct
    19441 tgtttactca cttgttcaag gtgctgcctt cgctatggct gacgtcgtaa acctacgtat
    19501 gcactcattc ggttcaatcg agttcttgac tcgtacacct attgcaatca gtgctggtat
    19561 tggtatggat atcgttaact tcgtttgggt aactgttctc tttgctgtaa tcatgtactt
    19621 tatcgcaaac ttcatgattc aaaaattcaa ctacgcaact ccagggcgca acggaaacta
    19681 cgaaactgct gaaggttcag aagaaaccag cagcgaagtg aaagttgcag caggctctca
    19741 agctgtaaac attatcaacc ttcttggtgg acgtgtaaac atcgttgatg ttgatgcatg
    19801 tatgactcgt cttcgtgtaa ctgttaaaga tgcagataaa gtaggaaatg cagagcaatg
    19861 gaaagcagaa ggagctatgg gtcttgtgat gaaaggacaa ggggttcaag ctatctacgg
    19921 tccaaaagct gacattttga aatctgatat ccaagatatc cttgattcag gtgaaatcat
    19981 tcctgaaact cttccaagcc aaatgactga agcacaacaa aacactgttc acttcaaaga
    20041 tcttactgag gaagtttact cagtagcaga cggtcaagtt gttgctttgg aacaagtaaa
    20101 ggatccagta tttgctcaaa aaatgatggg tgatggattt gcagtagaac ctgcaaatgg
    20161 aaacattgta tctccagttt caggtactgt gtcaagcatc ttcccaacaa aacatgcttt
    20221 tggtattgtg acggaagcag gtcttgaagt attggttcac attggtttgg acacagtaag
    20281 tcttgaaggt aaaccattta cagttcatgt tgctgaagga caaaaagttg cagcaggaga
    20341 tctccttgtc acagctgact tggatgctat ccgtgcagca ggacgtgaaa cttcaacagt
    20401 agttgtcttc acaaatggtg atgcaattaa atcagttaaa ttagaaaaaa caggttctct
    20461 tgcagctaaa acagcagttg ctaaagtaga attgtaatat acttgaggtt ggaagctgta
    20521 ttccaacctc ttattttggg agaaaagaat gaaattttta acactcaata ctcacagttg
    20581 gatggagaaa gaagcagagg aaaaattcca gattttgctt gaagatattc ttgaaaagga
    20641 ctatgatttg atttgtttcc aagaaatcaa tcaggagatg acctcgtcag aggtggaggt
    20701 taatgacctt tatcaagctt tgccagcagc tgagcctatt caccaagacc attatgttag
    20761 actcttggtt gaaaagttgt tagagcaagg gaaaaattac tactggacct gggcttataa
    20821 ccatatcggc tatgaccgct accacgaagg tgtggctatc ttgtctaaaa cacctattga
    20881 agccagagaa attttggttt cagatgtgga tgatccaaca gactatcata ctcgccgtgt
    20941 tgccctagct gaaactgtag tcgatggcaa ggagctagca gttgccagtg ttcatctctc
    21001 ttggtgggat aaaggtttcc aagaagaatg ggcacgattt gaggctgtct tgaaaaaatt
    21061 gaacaagcca cttttactag ctggagattt taacaaccca gcaggtcagg aaggttacca
    21121 agctatttta gctagtccat taggcttaca agacgcattt gaagttgctc aagagaaaag
    21181 tggtagctat actgttccgc ctgaaattga tggctggaaa gggaacactg aaccccttcg
    21241 aatcgattat gtctttacta ccaaagagtt agcggtggaa aatttacatg tcgtatttga
    21301 tggtaacaag agtccacaag tgagtgatca ctatggcttg aatgctatat taaactggaa
    21361 ataataactg aaaagaggtt ggaactataa aattccagcc ttttcttact agagaagcta
    21421 ctggaaatag cctaaataag tgagactact gtaatggaat aaaatatggt ataattgata
    21481 agatagatag aatcgaggat gttatgtcat ttacgaaatt tcaatttaaa aactatatta
    21541 gagaagcctt gaaggagtta aaatttacaa ctccaacaga ggtgcaagac aagttgattc
    21601 ctattgtttt ggcaggtcgt gacctagtag gagaatcaaa aacaggttca ggtaagactc
    21661 atactttctt gttaccgatt ttccagcaat tagatgaagc tagcgatagt gtacaagcag
    21721 tgattactgc accgagtcgt gagttggcta ctcaaattta ccaagtagcg cgtcagattt
    21781 cagctcactc agatgtcgaa gttcgtgtgg ttaattatgt gggtggtacg gataaggctc
    21841 gccagattga gaaattggaa agcaatcagc ctcatattgt tattggaaca ccaggccgta
    21901 tctacgactt ggttaaatct ggtgatttag ctattcataa agccaagaca tttgttgttg
    21961 atgaagcaga tatgaccttg gatatgggat tcttggaaac tgttgataag attgctggca
    22021 gtcttccaaa agacttgcaa ttcatggtct tctcagcgac tatcccacaa aaactgcaac
    22081 cattcttgaa aaaatactta tcaaatcctg ttatggagaa aattaagacc aaaacggtta
    22141 tttctgacac cattgataat tggttgattt cgaccaaggg acatgataag aatgctcaaa
    22201 tttaccagtt gactcagttg atgcagccgt atttggcaat gatttttgtt aacactaaaa
    22261 cgcgtgctga tgaattgcat tcatatctga ctgctcaagg cttgaaggtt gcaaaaatcc
    22321 atggagatat tgcccctcgt gaacgcaagc gaatcatgaa tcaggtgcaa aatctggatt
    22381 ttgagtatat tgtcgcaaca gatttagcag cgcgtgggat tgacattgaa ggtgtcagcc
    22441 atgtcatcaa tgatgccatt ccgcaagact tatctttttt tgttcatcgt gttggtcgta
    22501 ctggacgaaa tggcctacca ggtacagcta ttacccttta tcagccaagt gatgactcgg
    22561 atatccgtga gttggagaaa ttgggaatca agtttagtcc taagatggtc aaagacgggg
    22621 aatttcaaga tacctatgac cgtgatcgtc gtgccaaccg tgagaaaaaa caagataaac
    22681 ttgatatcga aatgattggt ttggttaaaa agaaaaagaa aaaagtcaaa ccgggttata
    22741 agaagaaaat tcaatgggcg gttgatgaaa agcgccgtaa aaccaagcgt gctgaaaatc
    22801 gcgctcgcgg tcgtgcagag cgtaaagcta aacgccaaac attttaatag aaattgttgg
    22861 agtattgagc tccaactttt ttatttatga gaacgaacta tctaaaccga aacactacat
    22921 taaagactgc aaattgcgat taaaaatggt ataatgataa agttatatag tcccgataag
    22981 atggtaggta tttattacga agagttttcc tatcagtact ttgtaactct ataacaatat
    23041 tttttaaggg gggacatttt tatgtcagaa cgtaaattat tcacgtctga atctgtatct
    23101 gaggggcatc cggataagat tgcagaccaa atttcagatg cgattttgga tgctatttta
    23161 gcaaaggatc cagaggcgca cgttgctgct gaaacagctg tatatactgg ttctgtccac
    23221 gtttttggtg aaatttctac aaatgcctat gtggatatta accgtgtggt tcgtgatacc
    23281 attgcagaga ttggttatac caatacagaa tatggatttt ctgctgagac ggtgggagta
    23341 cacccatctt tggtggaaca atctcctgac atcgctcaag gtgttaacga agccttggag
    23401 gttcgtggaa atgctgatca agatccactg gacttgattg gagcaggtga ccaagggctc
    23461 atgtttggat ttgcagtaga tgaaacagaa gagctcatgc cattgccaat tgcactcagt
    23521 cataaattgg ttcgtcgtct ggcagaactt cgtaagtctg gagaaattag ctatctccgt
    23581 ccagatgcaa aatcacaagt tacagttgag tacgatgaaa atgaccgtcc ggtacgtgta
    23641 gatacagtcg ttatttctac tcagcatgat ccagaggcca ctaatgaaca aatccatcaa
    23701 gatgtgatta acaaggtcat caaagaagtt attccatctt cttatcttga tgataagaca
    23761 aaattcttta tcaatccgac aggtcgtttt gtaatcggtg gtcctcaagg ggactcaggt
    23821 ttgactggtc gtaagattat tgtagatact tatggtggct actctcgtca tggtggtggt
    23881 gccttctctg gtaaagatgc gactaaggtg gatcgttcag cctcttatgc ggctcgctat
    23941 attgccaaga atatcgttgc agcaggcctt gctaagaagg cagaagtgca gttggcctat
    24001 gctatcggtg ttgcgcaacc tgtttctgtt cgtatcgata ctttcggtac aggaacagta
    24061 gctgaaagtc aacttgaaaa agcggctcgt caaatctttg accttcgccc tgcagggatt
    24121 atccaaatgc tggacctcaa gcgtccaatt taccgtcaaa catcggctta cggtcacatg
    24181 ggacgtacag atattgatct tccatgggaa cgtttggata aggtagatgc tttgaaagaa
    24241 gcagtaaaat aagattttaa gaggggaacg tcctctcttt tttatagttt ttaactatac
    24301 tgggatactg ttctgaaaat ccattttgcg aaagtagaga tttacatgta tagtagattg
    24361 aaactagaat agtacacctc aacttctaaa acattgttag caatcaattt gactgtcctg
    24421 atcgatttct cctgttcttg tttcatttta ctatatttct ttaaaaatga taaaggttga
    24481 gatttctcct cgtaatagat aatcatgggg atatttcaat ccaaagtttt attcgttatc
    24541 acttgactat tgcaaggttt tctagagcaa cagagtcatg gaatggactc atggttgaga
    24601 tttctccttg ttgcttggac ttcattcaaa agtctgttac ccaagccttg ttcaaacttc
    24661 caatacacta gctgttttca tagcatgact tctgtactag actttctttt ccgaataaat
    24721 agatagaacc acagaatcta gtaaacctag aattaaaatt atggtataat attagcaata
    24781 aaagaaatct ggaggattag aatcatggta tcaacgaaaa cacaaattgc tggttttgag
    24841 tttgacaatt gcttgatgaa tgcagcaggt gtggcttgta tgacgataga ggagttagaa
    24901 gaggtcaaaa actcagcggc aggaaccttt gttactaaga cagcgacctt ggacttccgt
    24961 caggggaatc ctgagccacg ctaccaagat gttccacttg gttccatcaa ctctatgggc
    25021 ttgccaaata atggcttaga ctattatttg gattatcttt tagatttgca ggaaaaagag
    25081 tcgaaccgaa ctttcttctt atctctggtc ggcatgtctc cagaggaaac ccatactatt
    25141 ttgaaaaaag ttcaagagag tgattttcgt ggtctgactg agctaaatct ttcctgtcca
    25201 aatgttccag gtaaacctca gattgcctat gattttgaga caacagaccg gattttggca
    25261 gaagtgttta cttacttcac caaacctctt ggaattaaat tgccacctta ttttgatatt
    25321 gttcactttg atcaagcggc agctattttc aacaaatatc cgctcaagtt tgtcaactgc
    25381 gttaactcta tcggaaacgg cctctatata gaagacgaat ctgtcgttat tcggcctaag
    25441 aatggttttg gtggaattgg tggagaatac atcaaaccga ctgctctagc caatgttcac
    25501 gccttttatc aacgtttaaa tcctcaaatc caaattatcg gaacaggtgg cgttctgact
    25561 ggtcgagatg cctttgaaca catcctctgt ggagcaagta tggtgcaggt gggaacgacc
    25621 cttcacaaag aaggcgtcag tgcttttgac cgcattacca atgaactgaa agcaatcatg
    25681 gtggaaaaag gctacgagag cttagaagat ttccgtggga aattgcgcta tattgactaa
    25741 attaaatcga aaaatctgaa gaaaggagag acgatgctag ccattgaaga aagtcagaag
    25801 ttgactttat caaatttacc gagcctgagc ctatttacag ggacagatca gggtcagttt
    25861 gaagtgatga agagtcaagt gttgaaacag attgggtatg attctgctga cctcaacttt
    25921 gcctactttg atatgaaaga agtagtttac aaggatgtgg aactggagtt ggtcagcctt
    25981 cctttctttg cggatgagaa aatcgtgata ttagattatt ttatggatat cacgactgct
    26041 aagaaacgct ttttgacaga tgatgagctt aagtcatttg aggaatacct tgacaatcct
    26101 tctccaacaa ccaagttgat aatctttgca gaaggaaagc tggatagcaa aagacggtta
    26161 gtcaaattac ttaagcgtga tgccaaggcc ttcgatgcag tagaagtaaa agaacaagaa
    26221 ttgcgccagt atttccaaaa gtggagtcag aaacaaggtc tgcagtttac caatcattct
    26281 tttgaaaatc tcctcatcaa gtcggggttt caatttagcg aaatccagaa aaatcttctc
    26341 tttttacagt cctataaggc ggattctgtt atcgaggaag aggatattgt taacgcaatt
    26401 cccaagacct tgcaggacaa tatttttgat ttaactcagt ttattctgac taaaaagatg
    26461 gatcaggcgc gcgatttggt gagagacttg accttgcaag gggaagatga aatcaaacta
    26521 attgcagtca tgctgggaca atttcggact tttactcagg tgaagatttt ggcggagtct
    26581 ggccaaacag aatcgcagat tgcaagtagt ttaggtagtt atctgggacg taacccaaat
    26641 ccttatcaaa tcaagtttgc attaagagat tcgagaggac tttctttgag ctttctgaag
    26701 caagctattt cctatttgat tgagacagac tatcagatta agacaggtct ttatgaaaaa
    26761 ggtttccttt ttgaaaaggc actcttacag attgctagtc aggtcaattg acatttgttg
    26821 aaactacaac ccgcgtcagg attatcttgt cgtgggtttc ttgctgattt gcttatctat
    26881 aagcaaaaaa cttgaaaaaa gtgaatgttt gcgttatcat tttcatatag aaagaaaaag
    26941 aggtattaca gatggctatt atcttaccag aacttccata tgcatacgac gctttggaac
    27001 catacatcga tgcggaaaca atgcatttgc accatgacaa acaccatcaa acttatgtca
    27061 acaatgccaa tgcagcttta gaaaaacacc ctgaaatcgg tgaagacctt gaagccttgc
    27121 ttgctgatgt agaatctatc ccagctgata tccgtcaagc acttatcaac aatggtggcg
    27181 gacacttgaa ccacgctctt ttctgggaat tgatgactcc cgagaaaaca gctccttcag
    27241 cagaactggc agcagcaatc gatgcaacat ttggttcatt tgaagaattc caagcagcct
    27301 tcactgcagc agcaacaact cgttttggtt caggttgggc atggttggtt gtcaacaaag
    27361 aagggaaact tgaagtgact tcaacagcaa accaagacac accaatctca gaaggtaaaa
    27421 aaccaatctt gggcttggac gtttgggaac atgcttacta cgtgaaatac cgcaacgtgc
    27481 gtcctgacta catcaaagct ttcttttcag taatcaactg gaataaagta gatgaattgt
    27541 acgcagctgc taaataatga tagttggagg gaagaattgt tcttctcttt tttaggttat
    27601 aagattctgg tctgacaaaa tcgttagact tttttcattt tttatgagaa acgtgctaac
    27661 tgctagaaat tatggtagaa taaagtatta agtaatcgtg gaaaaaggat atctatgcga
    27721 aaagaaattg cacctgaatt atacaactat aacaagtttc ctggtccgga gttccattta
    27781 cacggggaca aggtcgaaac ggaagggata gctttttcct tggttgaaaa tatcaaggat
    27841 gcctttgatg tgacgacttt taatcagcgt ttttcagaag tcttaaccaa gtttgattat
    27901 atcgtggggg actggagcaa cgaacagctt cgcctacgag gtttttacaa ggatgaccga
    27961 acagaagaaa aacttgaaaa aatcagtcgt ttacaagact accttttaga gtattgtagt
    28021 tatggttgtg cctattttgt cttagaaaat gaagccccta agcgagcatc atttgacaag
    28081 aaaatgcgta agacggaaga agaaacacct tctagaaaag gaaagaaacc gactcaaact
    28141 aaacgaaagt cgaatgcaga taagaaaaat agacgtcgtc agaaagacca gcattctcag
    28201 aaagaggaca aggaacaacg tcattttgtc attcgtcaga agtgagtaga gaataaggag
    28261 aagaaatgaa accctcaatt catagtttgg tacatcaaac catgcaggaa tgggtattgg
    28321 agcagggaga aaagaaattc cgtgcagatc aaatctggga atggctctac cgtaaacgtg
    28381 tgcagtcatt tgaagaaatg accaaccttt ccaaggattt gattgctaag ctcaatgacc
    28441 agtttgtggt caatcccttg aaacaacgta ttgtgcaaga gtctgctgat ggtacagtca
    28501 aatatctctt tgaattgcct gacggtatgt taattgagac tgtactcatg cgtcaacact
    28561 atggtttatc tgtctgtgtg accactcagg tcggctgtaa tatcggttgt accttctgtg
    28621 cctctggttt gattaagaag cagcgtgacc tcaataacgg tgaaatcgta gcgcaaatca
    28681 tgctggttca gaaatacttt gctgatgaag gggttcaggt caatcttgcc gtgtctcttc
    28741 acgcacccaa caatgaactt cgttcaagta tcatgaagat taaccgtgcc tttccgattg
    28801 aaaaactctt tgctgctatt gaatactaca ttgagacaac aaaccgtcgt gtaacctttg
    28861 aatatatcat gcttaatgaa gtcaacgacg gtgtagaaca agccttggaa ttggctgaat
    28921 tgctcaagaa catcaagaaa ttgtcttatg taaacttgat tccttataac ccagttagtg
    28981 agcatgacca atatagccgt agtcccaaag agcgcgtgtt ggctttctat gatacgctta
    29041 agaaaaaagg ggtcaactgt gtggttcgtc aagagcatgg tacagatatt gacgcagctt
    29101 gtggacaatt gcgctctaac acaatgaaac gtgaccgcca gaaggcagtc gcagcggtaa
    29161 atccttaatg atgaagaaga cttataatca tattttggtc tggggagtca ttttctatag
    29221 catttgcatt gtctgttttt gctttactcc tcaagaacaa tctaccgtgg gagtgggaac
    29281 tccaggtatt cagcatcttg gacgcctggt ttttcttttg actcctttca attctctctg
    29341 gaaactgggc gaagtgagtg acattggaca attatgttgg atttttttac aaaatgtcct
    29401 caatgtcttc ttgttttttc ctctgatttt ccaactcctt tatctatttc caaatttgcg
    29461 gaaaacaaaa aaggtccttc tttttagttt tcttgtgagt cttggaatcg agtgtacgca
    29521 attaatcttg gactttttct ttgatttcaa tcgcgtcttt gagattgatg atttgtggac
    29581 caatactttg ggtggctatc tggcttggct cctttataaa cgattacata aaaacaagat
    29641 aaggaattaa aatgagtatt ttagaagtta aaaatctgag tcacggtttt ggtgaccgtg
    29701 caatttttga agatgtgtcc ttccgtctcc tcaagggaga acatatcggc ctggtcggtg
    29761 ccaatggtga aggaaaatca acctttatga gtatcgtgac tggtaaaatg ctgccagatg
    29821 aaggaaaggt tgagtggtcc aaatatgtga cggctggtta cttggatcag cactctgtcc
    29881 ttgctgaaag acagtcggtg cgtgatgttc tccgtacggc ttttgatgag cttttcaaag
    29941 ctgaagctcg tatcaatgac ctctatatga aaatggctga agacggcgcg gatgttgatg
    30001 ctctcatgga agaagtagga gaacttcaag accgtctgga gagtcgtgat ttctatacct
    30061 tggatgctaa gattgacgaa gtagcgcgtg ctcttggtgt tatggacttt ggcatggata
    30121 cggatgtaac ttctttgtca ggtgggcaaa gaaccaaggt gcttttggca aaacttctcc
    30181 ttgaaaagcc tgatatcttg ctgttggacg agccgaccaa ctacttggat gctgagcata
    30241 ttgattggct caagcgctat ctccaaaact atgagaatgc ctttgttctc atttcgcacg
    30301 atattccatt cctcaatgac gttattaata ttgtctatca tgtggaaaat caacagctga
    30361 cgcgttactc tggtgactac taccagttcc aagaagttta tgctatgaag aaatctcagc
    30421 tagaggcagc ctacgaacgc cagcagaaag agattgcaga cctcaaggac tttgtggctc
    30481 gtaataaagc ccgtgttgca actcgtaata tggctatgtc tcgtcaaaag aaattggata
    30541 agatggatat tatcgaactc caaagtgaga aaccaaaacc atcctttgat ttcaaaccag
    30601 ctcgtacacc agggcgcttt atcttccaag ccaagaactt gcaaattggt tacgaccgtc
    30661 ctcttactaa gcctttaaat cttaccttcg aacgcaatca aaaggttgcg attattggtg
    30721 ctaatggtat tggaaaaaca actctcttga agagtctctt gggcattatc tcgccaatcg
    30781 ctggggaagt ggagcgtgga gattatttag aacttggtta ttttgagcag gaagtagaag
    30841 gtggtaatcg ccaaactcct cttgaagctg tctggaatgc ctttcctgcc cttaatcaag
    30901 cagaagtccg tgcagccctt gcccgttgtg gtttgacaac caaacatatt gaaagccaga
    30961 ttcaagtatt atcaggggga gagcaagcca aggttcgttt ctgtctcttg atgaatcgtg
    31021 aaaacaacgt tttagtgctg gacgagccga ccaaccattt ggatgtggat gcaaaggatg
    31081 agctcaaacg cgctctcaaa gaatacaggg gatctatcct tatggtctgc cacgagccag
    31141 acttttatga aggttggata gaccaaatat gggattttaa taatttaact taagaatgag
    31201 agtaaaagaa atacggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
    31261 aggacaaaat tcgtcctttc ttttttgatg ttcagagcga taaaaatcct ttttttgaag
    31321 ttttcaaagt tccgaaatcc aaaggcgttt cgtttgataa gtttgatgag attattggtc
    31381 gcttccaatt ttgcgttgga ataaggtaat tgaagagcgt tgacgatttt ttctttgt
//
LOCUS       DAVLSL010000007        29505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLSL010000007 DAVLSL010000000
VERSION     DAVLSL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.007"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            17..460
                     /locus_tag="VXN30_000256"
     CDS             17..460
                     /locus_tag="VXN30_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1356936.1"
                     /translation="MFCLEADNQEHLFDQVATLLEEREIVTPTYREALITREKSFPTG
                     LDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFII
                     NNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            498..803
                     /locus_tag="VXN30_000257"
     CDS             498..803
                     /locus_tag="VXN30_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1356937.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            851..2326
                     /locus_tag="VXN30_000258"
     CDS             851..2326
                     /locus_tag="VXN30_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1356938.1"
                     /translation="MEAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLRGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2676..9377
                     /locus_tag="VXN30_000259"
     CDS             2676..9377
                     /locus_tag="VXN30_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1356939.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWHF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNNFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SSQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFLYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9625..10481)
                     /locus_tag="VXN30_000260"
                     /pseudo
     CDS             complement(9625..10481)
                     /locus_tag="VXN30_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10692..11051)
                     /locus_tag="VXN30_000261"
     CDS             complement(10692..11051)
                     /locus_tag="VXN30_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1356940.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            11143..12099
                     /locus_tag="VXN30_000262"
                     /pseudo
     CDS             11143..12099
                     /locus_tag="VXN30_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            12083..12640
                     /locus_tag="VXN30_000263"
     CDS             12083..12640
                     /locus_tag="VXN30_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1356941.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12633..12887
                     /locus_tag="VXN30_000264"
     CDS             12633..12887
                     /locus_tag="VXN30_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356942.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12899..14953
                     /locus_tag="VXN30_000265"
     CDS             12899..14953
                     /locus_tag="VXN30_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1356943.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            15132..15992
                     /locus_tag="VXN30_000266"
     CDS             15132..15992
                     /locus_tag="VXN30_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1356944.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            16203..16913
                     /gene="ccdA2"
                     /locus_tag="VXN30_000267"
     CDS             16203..16913
                     /gene="ccdA2"
                     /locus_tag="VXN30_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1356945.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16933..17499
                     /locus_tag="VXN30_000268"
     CDS             16933..17499
                     /locus_tag="VXN30_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1356946.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17510..18622
                     /gene="msrB"
                     /locus_tag="VXN30_000269"
     CDS             17510..18622
                     /gene="msrB"
                     /locus_tag="VXN30_000269"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1356947.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18890..19627
                     /locus_tag="VXN30_000270"
     CDS             18890..19627
                     /locus_tag="VXN30_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1356948.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19624..21315
                     /locus_tag="VXN30_000271"
     CDS             19624..21315
                     /locus_tag="VXN30_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1356949.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPDHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            21431..21994
                     /locus_tag="VXN30_000272"
     CDS             21431..21994
                     /locus_tag="VXN30_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1356950.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            22016..27685
                     /locus_tag="VXN30_000273"
     CDS             22016..27685
                     /locus_tag="VXN30_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1356951.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVMYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            27746..29467
                     /gene="pabB"
                     /locus_tag="VXN30_000274"
     CDS             27746..29467
                     /gene="pabB"
                     /locus_tag="VXN30_000274"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1356952.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGFLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
BASE COUNT         9229 a         5373 c         6488 g         8415 t
ORIGIN      
        1 ctttgacaaa aaccttgtgt tttgcttaga agcggataat caagaacatc tctttgatca
       61 ggttgcaact ttattggaag aacgagaaat tgtaactcca acttatcgtg aagccttgat
      121 cacgcgtgaa aagtcatttc caactggttt agatatggaa tttctaggaa aggacttgcc
      181 aaatgtagcg attcctcaca cagatattgt tcataatcta gctgagaaag tggtggttgt
      241 tcgattagaa aaaccagtaa cttttcacaa tatgatagct cctgataagg aagtagaagt
      301 atctctactc ttctttatca ttaacaattc aagttcaagt caaacgaaca ttctggctca
      361 gttgatggac tttttcacag gaaatggtca tcttgaagac ctatcaaaaa tttctgaacc
      421 agaaaaactt tatgcttaca ttgctgaagc aaccgcttaa tcttgtctat taaaaaataa
      481 aatcggagga aatccaaatg attaaaattc ttgctgcctg cggtgcaggt gttaactcaa
      541 gtcaccaaat taaaagtgct ctagaagaag aactttcaaa ccgtggttat gatgttcact
      601 gtgatgcagt catggtgaaa gatgtaaacg aagaccttat gaaaggttac gatatcttta
      661 caccaatcgc tgcaacagac cttggttttg aaccaggtat cccagttatc gaagctgggc
      721 caatcttatt ccgtatccca gcaatgagcg ctccagtatt tgacaatatt gaagcagcta
      781 ttaaagaaca tggattaagc taattctaat ttgttaacat tcatataaca taaaaaaggg
      841 aggaactgtt atggaagcaa tctttgacct aatcggaaag gttttcaatc ccatcttaga
      901 aatgggtgga cctgtcatca tgttgatcat tttgacagta ttggctttac tttttggagt
      961 gaaattctcc aaagcgcttg aaggtggtat caaacttgcc atcgctctta caggtatcgg
     1021 tgctatcatc ggtatgctaa atggtgcttt ctcagcatca cttgcaaaat tcgttgaaaa
     1081 cactggtatc caattgagta ttacagacgt tggttgggca ccactcgcaa caatcacttg
     1141 gggttctgct tggacactat acttcttgct catcatgttg attgtcaacg tagtgatgct
     1201 tgctatgaag aaaaccgata cacttgatgt cgatatcttt gatatctggc acttgtctat
     1261 cacaggtctc ttgattaaat ggtatgctga taacaatggt gtgagtcaag gggtttcact
     1321 ctttattgct acagcagcta tcgtccttgt cggtgtgttg aaaattatca actctgactt
     1381 gatgaaacct acatttgatg accttcttaa cgccccaagt tcatcaccaa tgacatcaac
     1441 tcacatgaac tacatgatga acccagttat catggttttg gataagattt ttgaaaaatt
     1501 cttcccaggc cttgataaat atgactttga tgctgctaaa ttgaacaaga aaatcggttt
     1561 ctggggatct aaattcttca tcggtttcat ccttggtatc gttatcggta ttatgggaac
     1621 tccacatcca attgcaggtg ttgcagatgc agataaatgg cgtcttgtta tcaaaggatg
     1681 gttgtctctt ggtttgactg ccggtgtatc tttggaactc ttctcactta tcggttcatg
     1741 gttcatcgca gccgtagaac cactatcaca aggtattaca aacgttgcta ctaaacgtct
     1801 tcgaggacgt aaattcaata tcggtcttga ctggccattc atcgctggtc gtgctgaaat
     1861 ctgggcttgt gccaacgtac ttgcaccaat catgttgatt gaagcagtgc ttctttcaaa
     1921 agttggaaat ggtatcttgc cacttgcagg tatcatcgct atgggtgtta ctccagctct
     1981 cttggttgta actcgtggta aattgctccg tatgattatc ttcggaacac tcttgttgcc
     2041 actcttcctt ctttcaggta cacttattgc accatttgca acagaacttg ctaaaggtgt
     2101 aggtgccttc ccagaaggtg tgagccaaac tcaattgatt actcactcta ctcttgaagg
     2161 accaatcgaa aaacttcttg gttggacaat tggtaacact acaactggtg atatcaaagc
     2221 aatccttggt gcagtagtct tccttgtatt ctatatcggt atctttgctt ggtacagaaa
     2281 acaaatgatc aaacgtaacg aagagtacgc agcaaaagca aaataatgcg ctcctataaa
     2341 atataaactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggt tgctaaaaac
     2401 acagttttga ggttgtagat aagactgacg aagtcagctc aaaacatggt tttgaggttg
     2461 tagatgaaac tgacgaagtc agctcaaaac atggttttga ggttgtagat gaaactgacg
     2521 aagtcagtaa tcatatctac ggcaaggcga cgctgacgaa gtttgaattg gattttcgca
     2581 gagtgtaaat tgttgaaact aggttgtcat accatgtata ccacttgggc tttggtagta
     2641 gctaactgca ctaaatataa tataaggagg gttttatggg gaaaggccat tggaatcgga
     2701 aaagagttta tagcattcgt aagtttgctg tgggagcttg ctcagtaatg attgggactt
     2761 gtgcagttct attaggagga aatatagctg gagaatctgt agtttatgcg gatgaaacac
     2821 ttattactca tactgctgag aaacctaaag aggaaaaaat gatagtagaa gaaaaggctg
     2881 ataaagcttt ggaaactaaa aatgtagttg aaaggacaga acaaagtgaa cctagttcaa
     2941 ctgaggctat tgcatctgag aagaaagaag atgaagccgt aactccaaaa gaggaaaaag
     3001 tgtctgctaa accggaagaa aaagctccaa ggatagaatc acaagcttca agtcaagaaa
     3061 aaccgctcaa ggaagatgct aaagctgtaa caaatgaaga aatgaatcaa atgattgaag
     3121 acaggaaagt ggattttaat caaaattggc actttaaact caatgcaaat tctaaggaag
     3181 ccattaaacc tgatgcagac gtatctacgt ggaaaaaatt agatttaccg tatgactgga
     3241 gtatctttaa caatttcgat catgaatctc ctgcacaaaa tgaaggtgga caactcaacg
     3301 gtggggaagc ttggtatcgc aagactttca aactagatga aaaagacctc aagaaaaatg
     3361 ttcgccttac ttttgatggc gtctacatgg attctcaagt ttatgtcaat ggtcagttag
     3421 tggggcatta tccaaatggt tataaccagt tctcatatga tatcaccaaa taccttcaca
     3481 aagatggtcg tgagaatgtg attgctgtcc atgcagtcaa caaacagcca agtagccgtt
     3541 ggtattcagg aagtggtatc tatcgtgatg tgactttaca agtgacagat aaggtgcatg
     3601 ttgagaaaaa tgggacaact attttaacac caaaacttga agaacaacaa catggcaagg
     3661 ttgaaactca tgtgaccagc aaaatcgtca atacggacga caaagaccat gaacttgtag
     3721 ccgaatatca aatcgttgaa cgaggtggtc atgctgtaac aggcttagtt cgtacagcga
     3781 gtcgtacctt aaaagcacat gaatcaacaa gcctagatgc gattttagaa gttgaaagac
     3841 caaaactctg gaccgtttta aatgacaaac ctgccttgta cgaattgatt acgcgtgttt
     3901 accgtgacgg tcaattggtt gatgctaaga aggatttgtt tggttaccgt tactatcact
     3961 ggactccaaa tgaaggtttc tctttgaatg gtgaacgtat taaattccat ggagtatcct
     4021 tgcaccacga ccatggggcg cttggagcag aagaaaacta taaagcagaa tatcgccgtc
     4081 tcaaacaaat gaaggagatg ggagttaact ctatccgtac aacccacaac cctgctagtg
     4141 agcaaacctt gcaaatcgca gcagaactag gtttactcgt tcaggaagag gcctttgata
     4201 cgtggtatgg tggcaagaaa ccttatgact atggacgttt ctttgaaaaa gatgccactc
     4261 acccagaagc tcgaaaaggt gaaaaatggt ctgattttga cctacgtacc atggtcgaaa
     4321 gaggcaaaaa caaccctgct atcttcatgt ggtcaattgg taatgaaata ggtgaagcta
     4381 atggtgatgc ccactcttta gcaactgtta aacgtttggt caaggttatc aaggatgttg
     4441 ataagactcg ctatgttacc atgggagcag ataaattccg tttcggcaat ggtagcggag
     4501 ggcatgagaa aattgctgat gaactcgatg ctgttggatt taactattct gaagataatt
     4561 acaaagccct tagagctaag catccaaaat ggttgattta cggttcagaa acatcatcag
     4621 caacccgtac acgaggaagt tactatcgcc ctgaacgtga attgaaacat agcaatggac
     4681 ctgagcgtaa ttatgaacag tcagattatg gaaatgatcg tgtgggttgg gggaaaacag
     4741 caaccgcttc atggactttt gaccgtgaca acgctggcta tgctggacag tttatctgga
     4801 caggtacgga ctatattggt gaacctacac catggcacaa ccaaaatcaa actcctgtta
     4861 agagctctta ctttggtatc gtagatacag ccggcattcc aaaacatgac ttctatctct
     4921 accaaagcca atgggtttct gttaagaaga aaccgatggt acaccttctt cctcactgga
     4981 actgggaaaa caaagaatta gcatccaaag tagctgactc agaaggtaag attccagttc
     5041 gtgcttattc gaatgcttct agtgtagaat tgttcttgaa tggaaaatct cttggtctta
     5101 agactttcaa taaaaaacaa accagcgatg ggcggactta ccaagaaggt gcaaatgcta
     5161 atgaacttta tcttgaatgg aaagttgcct atcaaccagg taccttggaa gcaattgctc
     5221 gtgatgaatc tggcaaggaa attgctcgag ataagattac gactgctggt aagccagcgg
     5281 cagttcgtct tattaaggaa gaccatgcga ttgcagcaga tggaaaagac ttgacttaca
     5341 tctactatga aattgttgac agccagggga atgtggttcc aactgctaat aatctggttc
     5401 gcttccaatt gcatggccaa ggtcaactgg tcggtgtaga taacggagaa caagccagcc
     5461 gtgaacgcta taaggcgcaa gcagatggtt cttggattcg taaagcattt aatggtaaag
     5521 gtgttgccat tgtcaaatca actgaacaag cagggaaatt cacccttact gctcactctg
     5581 atctcttgaa atctagtcaa gtcactgtct ttactggtaa gaaagaagga caagaaaaga
     5641 ctgttttggg gacagaggtg ccaaaagtac agaccattat tggagaggca cctgaaatgc
     5701 ctaccactgt tccgtttcta tacagtgatg gtagtcgtgc agaacgtcct gtaacctggt
     5761 cttcagtaga tgtgagcaag cctggtattg taacggtgaa aggtatggct gacggacgag
     5821 aagtagaagc tcgtgtagaa gtgattgctc ttaaatcaga gctaccagtt gtgaaacgta
     5881 ttgctccaaa tactgacttg aattctgtag acaaatctgt ttcctatgtt ttgactgatg
     5941 gaagtgtaca agagtacgaa gtagatagct gggagattac ggaagtagat aaagccaaac
     6001 tttcagtagc tggatcacgt attcaaatga ctggtcagtt agctggagaa actattcatg
     6061 caacccttgt ggtagaagaa ggaaatgctg cagcacctgt agtgccaact gttactgttg
     6121 gaggtgaagc agtaacaggt cttactagtc gacaaccaat gcaatatcgt actctatctt
     6181 atggtgccca attgccagaa gtcacagcaa gtgctgaaaa tgctgatgtg acagttcttc
     6241 aagcaagcgc agcaaacggc atgcgtgcaa gcatatttgt tcagcctaaa gatggtggcc
     6301 ctcttcaaac ctatgcaatt caattcctag aagaagcacc aaaaattgct cacttgagct
     6361 tgcaagtgga aaaagctgac agtctcaaag aagaccaaac tgtcaaattg tcggttcgag
     6421 ctcactatca agatggaacg caagctgtat taccagctga taaagtaacc ttctctacaa
     6481 gtggtgaagg ggaagtcgca attcgtaaag gaatgcttga gttgcataag ccaggagcag
     6541 tcactctcaa agcggaatat gagggagcta aaggccaagt tgaactcact atccaagcca
     6601 atactgagaa gaagattgcg caatctatcc gtcctgtaaa tgtagtcaca gatttgcatc
     6661 aaaaacctac tcttccaaca acagtaacgg ttgagtatga caaaggtttc cctaaagctc
     6721 acaaagtcac ttggcaagct attccgaaag aaaaactaga ctcctatcaa acatttgaag
     6781 tactaggtaa agttgaagga attgaccttg aagcgcgtgc aaaagtctct gtagaaggta
     6841 tcgtttcagt tgaagaagtc agtgtgacaa ctccaatcgc agaagcacca caattaccag
     6901 aaagtgttcg gacatatgat tcaaatggtc acgtttcatc agctaaggtt gcatgggatg
     6961 cgattcgtcc agagcaatac gctaaggaag gtgtctttac agttaatggt cgcttagaag
     7021 gtactcaatt aacaactaaa cttcatgttc gcgtatctgc tcaaactgag caaggtgcaa
     7081 acatttctga ccaatggacc ggttcagaat tgccacttgc ctttgcttca gactcaaatc
     7141 caagcgaccc agtttcaaat gttaatgaca agctcatttc ctacaataac caaccagcca
     7201 atcgttggac aaactggaat cgtactaatc cagaagcttc agtcggtgtc ctattcggag
     7261 attcaggtat tttgagcaaa cgttcagttg ataacttaag cgttggcttc catgaagacc
     7321 atggagttgg tgcaccgaag tcttatgtga ttgagtatta tgttggtaag actgtcccaa
     7381 cagctcctaa aaaccctagt tttgttggta atgaggacca tgtctttaat gattctgcca
     7441 actggaaacc agttactaat ctaaaagccc ctgctcaact caaggctgga gaaatgaacc
     7501 actttagctt tgataaagtt gaaacctatg ctgttcgtat tcgcatggtg agagcagaca
     7561 acaaactagg aacgtctatc acagaggtac aaatctttgc gaaacaagtt gcggcagcca
     7621 agcaaggaca aacaagaatc caagttgacg gtaaagactt agcaaacttc aaccctgatt
     7681 tgacagacta ctaccttgag tctgtagatg gaaaagttac ggcagtcaca gcaagtgtta
     7741 gcaacaatgg cctcgctacc gtcgttccaa gcgttcgtga aggtgagccg gttcgtgtca
     7801 tcgcgaaagc tgaaaatggc gacatcttag gagaataccg tctgcacttc actaaggata
     7861 agaacttact ttctcataaa ccagttgctg cggttaaaca agctcgcttg ctacaagtag
     7921 gtcaagcact tgaattgccg actaaggttc cagtttactt cacaggtaaa gacggctacg
     7981 aaacaaaaaa cctgacagtt gaatgggaag aagttccagc ggaaaatctg ataaaagcag
     8041 gtcaatttac cgttcgaggc catgtccttg gtagtgatct tgttgctgag gtcactgtac
     8101 gagtgacaga caaactaggc gaagctcttt cagataaccc taactatgat gaaaacagta
     8161 accaggcctt tgcttcagca accaatgata ttgacaaaaa ctctcatgac cgcgttgact
     8221 atctcaatga cggagatcat tcagaaaatc gtcgttggac aaactggtct ccaacaccat
     8281 cttctaatcc agaagtatca gcgggtgtga tcttccgtga aaatggtaag attgtagaac
     8341 ggactgttgc tcaagccaaa cttcacttct ttgcagatag tggtacggat gcaccaacta
     8401 aactcgtttt agaacgctat gtcggtccag agtttgaagt gccaacctac tattcaaact
     8461 accaagccta cgacgcagac catccattca acaatccaga aaattgggaa gctgtgcctt
     8521 atcgtgcgga taaagacatc gcagctggtg atgaaatcaa cgtaacattt aaagctgtca
     8581 aagccaaagc tatgagatgg cgtatggagc gtaaagcaga taagagcggt gttgcgatga
     8641 ttgagatgac cttccttgca ccaagtgaat tgcctcaaga aagcacgcaa tcgaagattc
     8701 ttgtagatgg aaaagaactt gctgatttcg ctgaaaatcg tcaagactat caaattacct
     8761 ataaaggtca acggccaaaa gtctcagttg aagaaaacaa tcaagtagct tcaactgtgg
     8821 tagatagtgg agaagatagc cttccagtac ttgttcgcct cgtttcagaa agtggaaaac
     8881 aagtcaagga ataccgtatc cagttgacta aggaaaaacc agtttctgag aagacagttg
     8941 ctgctgtaca agaagatctt ccaaaactcg aatttgttga aaaagatttg gcctacaaga
     9001 cagttgagaa aaaagattca acactgtatc taggtgaaac tcgtgtagaa caagaaggaa
     9061 aaactggtaa agaacgtatc tttacagcga ttaatcctga tggaagtaag gaagaaaaac
     9121 tccgtgaagt ggtagaagct ccgacagacc gcatcgtctt ggttggaacc aaaccagtag
     9181 ctcaagaagc taaaaaacca caagtgtcag aaaaagcaga tacaaaacca attgattcaa
     9241 gtgaagctag tcaaactaat aaagcccagt taccaaatac aggtagtgcg gcaagccaag
     9301 cagcagtagc agcaggttta gctcttctag gtttgagtgc aggattagta gttactaaag
     9361 gtaaaaaaga agactagaaa ttttgatacc ttctttatca ttaaagacca aagaaaaact
     9421 cttgtcctaa atagaagata aagagtctgg aacaaaatag ttctcaacca caaaaaagct
     9481 agatatttcc aattgcggaa ctctagcttt ttaattttga gtcgttctct tattgtatag
     9541 tagattgaaa ctagagtagt acacttctac ttctaaaaca ttgttagaaa tcgatttgac
     9601 tgtccttatc gatttgtcct gttcttattt catttcacta tgttttagga ggttattggc
     9661 cataagtacc aatcccatgt caattctcac ttgacgcttg cctctcagat tacatctctt
     9721 gtaacccaaa caagccttta tctgcccaaa gacaggttct acatcaatct tgcgttgagc
     9781 gaaaatctgt ctatcttggg gagataaaag cgcctgacat tctttagctt tcaagttttg
     9841 atagcgttcg ttcatataca atcccttttg aggggctgat tcaggttcat cagcgtagta
     9901 aaccttgatt tcctgttgaa agtctgtctg tgttttctgg tacttgatat ggtgaaaacg
     9961 atagcaccag ccatcaggat gtatgtagct atcctccttg tcattatagt gccaattcgc
    10021 taagttttta gctgactgtt tataccctct cttttgttcc ttatcaaaca tggcatattt
    10081 aatcagaagg ggagtgattt cttcttgaaa atagacttgt atctgttctt gaagtttggc
    10141 ggagaatttc tctgtcgttt tcttccacac aaaactatac ttgttggcat tggattcaat
    10201 cttagttccg tcaataaaca aacaatctaa ggtcactaac tcttccattt ttcaattttt
    10261 cgaccagaga aaatcccttg tgaataggca aatagaagag tagatacaag cattttaggg
    10321 tgataagacg ggcgaccaaa ggcatgatag gaggtgtaga agtgacgttc ctccaaggta
    10381 ttcaccactt tttcaatagt aaagacgaga tggtcttgtg gtaagaagga actgatttct
    10441 agtggtaaag ttgtttgatt tgtgttatag tgaatatgca tgggatagcc tttctaaatg
    10501 gtttattaag caattctatt ttaccaagac tatcgtaacc atgcaaaaaa gcaacggcaa
    10561 atccgttgcc ttttttggat ataagagact attgtcccag actcttttct ttgtataaaa
    10621 ttgtatagtg ttattgtcta tactattttg tagagaaagc aaaaatccct agaaaatgct
    10681 tagggattcg actatttcat taagaacaat tcaacaatca tctctatata catgataagg
    10741 gttaataaaa atcctgtacg ccagaagaat ttgatgaatt taggatagta aaagctgcgt
    10801 ttttttaaaa ggaagaaaaa gacgagaata atggctagga acgagagggc tagccccagt
    10861 ctagggagga aattatgggt aaaggtttta gctgtaatca agtaatactc aaataccaag
    10921 actggaaaag ccaaatccgc aaagtttcgt cctagttttt ttaatctaaa aagtttggtt
    10981 aggataatgc agacgactaa ggtcagtatc aataataaaa tagatgctaa tttcattaaa
    11041 atcataccca tattgtatca taaaaaaagg ctaatggaaa cgagaaacag aggaatttat
    11101 aggaaagtca ggcttttgag attgtgggtg atttttgtta taatgaaagt tataaaatct
    11161 tatgatacct tgaatgatta ttatcgaaaa ctctttggag aaaagacttt taaagtccct
    11221 attgatgcgg gatttgactg tcccaatcgt gatggaactg tggctcatgg aggctgtact
    11281 ttttgtacgg tttccggttc tggagatgcc attgtagccc cagatccgcc tatccgtgag
    11341 caattttata aggaaattga ctttattcat cgtaagtggc cagatgttca gaagtatctg
    11401 gtttattttc aaaattttac caacacccat gaaaaggtgg aagttattcg agagcgttat
    11461 gaacaggcta tcaatgagcc aggtgtggta ggaatcaata ttggaacgcg cccagactgt
    11521 ttaccagacg aaaccatcga atatttggct gagttatcgg agtgcatgca tgtgacggtt
    11581 gaattgggct tgcagactac ttatgaagca acctctgacc tgattaaccg tgcgcattct
    11641 tatgaattgt aggtggaaac ggtcaagcgt ttgagaaagt atccaaaaat tgagattgtt
    11701 tcccatttga tcaatggttt gcctggtgaa acccatgaga tgatggttga aaatgtccgc
    11761 cgttgtgtca cggataacga tattcaaggg attaaactgc atttgctcca tcttatgaca
    11821 aatactcgta tgcaacgaga ttaccatgag ggacgtttac aattgatgag tcaggacgaa
    11881 tatgtcaggg tcatctgtga ccaactggaa attattccca aacatatcgt catccatcga
    11941 attacaggag atgcgcctag ggatatgctg attggtccta tgtggagcct caataaatgg
    12001 gaagtcctca acagcattga gatggagatg agacgtcgtg gaagtgttca aggatgcaag
    12061 gctgtaaaac aggagtttga aaatgaaaag accacttgag atggcacatg attttttggc
    12121 tgaggtcgtg acaaaagagg atgtcgtagt ggatgcgact atgggaaatg gtcatgacac
    12181 gcttttttta gccaagctag ccaagcaagt ctatgccttt gatattcaga agcaagcctt
    12241 ggaaaagacc caagagcgtt tgcatcaggc tggcttgaca aatgcccagt taatcttgca
    12301 aggccatgag acactggacc agtttgtgac aaaagctaag gcagggattt ttaatttggg
    12361 ctatttgccg tcagctgata agtctgtcat cacccgaccg cagacaacga ttgaggcatt
    12421 agaaaagcta tgtggcttac ttgtcaaagg tggacgaatt gctattatga tttactatgg
    12481 tcatgaagga ggcgacctcg agagagatgc tgtcttggat tttgtgatcc agttgaacca
    12541 acaagagtac acagctgcca tttaccgaac tttaaaccaa gtcaacaacc cgccgttttt
    12601 agtgatgatt gaaaaattag agagatacag acatggataa acaataccta cacgaaaagc
    12661 tggatgccat gcgccagaat tttgttgaat caacctacca cgaacgagcg gtgggcgtgc
    12721 tagaccaagc gcatatgagc aaaaaaatgc ttaaaatcaa gaaaaaatta gttgctcttg
    12781 aaatggaacg gtgccagaga aaaattgagc acaaagactg ttccaagatt gaccaaaaaa
    12841 tcaaagagca gaaggagata tttgaatcct gttgtaaaaa agattaagga ggacgtgcgt
    12901 ggaattactg atttacctca tcctattttt actggtcttg attgtctcaa gtacaaccaa
    12961 taagctccta ccctttttgc ctctcccttt ggtgcagatt cttttgggaa ttgtgattgg
    13021 tctcttttta cccaatactg actttcacct taatacggag ttgtttttgg cactggttat
    13081 cggccccttg cttttccgag aggctgaaga agcagatgtt acggctattt taaaacactg
    13141 gcgaatcatt gtttatctca tatttccagt gatttttatc tcgaccctga gtttgggtgg
    13201 cttggcccat cttctttggt tcagccttcc cttggcagct tgcttggctg ttggggcagc
    13261 ccttggtcct acggacttgg tggcctttgc ctctctttcg gagcgtttta gctttcctaa
    13321 gcgcgtgtcc aatattctta agggcgaagg actcttgaat gatgcttctg gtttggtggc
    13381 ttttcaggta gctttgacag cttggacaac tggagctttt tctctggggc aagctagcag
    13441 ttcactcatc ttttcaatcc taggcggttt tttaattgga tttttaacag ccatgaccaa
    13501 ccgcttcctc cataccttct tgctaagtgt gcgtgcaacg gatattgcca gtgaactttt
    13561 attagaattg agtttgcctc ttgtgacctt ctttctggca gaagaagttc atgtttcagg
    13621 tattattgcc gtcgtagttg ctggaatttt aaaggcaagt cgtttcaaga aaatcacgct
    13681 cctcgaagcc caagtggata cggtgaccga gacggtctgg catacagtga cctttatgct
    13741 caacggttct gtctttgtga ttttagggat ggagctggaa atgatagcag aacctatctt
    13801 gaccaatcca atctataatc ctctactttt attgctatct cttatcgccc ttacctttgt
    13861 cctctttgtc attcgtttta ttatgatcta tggctattat gcctatagaa cccgacgcct
    13921 caagaaaaag ctaaataagt atatgaagga catgtttctc ttgacctttt caggtgttaa
    13981 gggaacggtg tcgattgcta cgattctctt gataccaagt aatctagaac aggagtatcc
    14041 tctcttgctt ttccttgttg caggtgtgac gcttgttagc tttttaacag gtctcttggt
    14101 cttgcctcat ctttctgatg aagaggaaga aagcaaggat tatctcatgc atatcgccat
    14161 tttgaatgaa gtaacgctag agttggaaaa agagttggaa gacaccagaa ataaactttc
    14221 cctctatgcg gctattgaca attatcatgg acgtattgaa aatctcattt taagccaaga
    14281 aaaccaggat gatcaagaag actgggctgc tttgaaactc ttgattctta gtattgaaag
    14341 tgatggtttg gaacaggcct atgaagaggg gaatattagc aatcgtgctt accgagttta
    14401 ccaacgttat ctgaaaaata tagaacaagg aatcaatcgt aaacttgcct caagactgac
    14461 ctattatttt cttgtttcct tgaggatttt acgttttctc cttcatgaag tttttactct
    14521 tggaaagacc ttccgtagct ggaaggacaa ggagcaaagc cgtctccgtg ctcttgatta
    14581 tgaccaaatt gcagagctct atcttgccaa tacagagatg attattgaaa gtttggaaaa
    14641 cctgaaggga gtctacagac gctctttgat tagttttatg caggagtctc gtcttcgaga
    14701 aacagctatt atcagcagtg gtgcctttgt cgaacgggtt atcaatcgtg tcaaacccaa
    14761 caatatcgat gaaatgctga gaggctatta tctggagcgc aagttgattt tcgaatacga
    14821 agaaaaacga ttaattacaa ctaagtatgc caagaaatta cgacaaaatg taaataactt
    14881 agagaactat tccttgaagg aagctgccaa taccctgccg tatgatatgg tggaattggt
    14941 aagaagaaat tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttgg
    15001 attatatatg tgactgactt cgtcagtttc atctacaacc tcaaagcagg gctttgagca
    15061 acctgcggct agcttcctag tttgctcttt gattttcatt gagtataaga ttataaatga
    15121 aggagtatga catgaaaaag tggtggaaag agctgataga caagccttta ttaaaagctt
    15181 ttttgcatta ttatcaagca tcagatagtg agttgaccag tgtcgcagta gcctactatt
    15241 ggttgatttc gattttcccc ctgcttttgg tggtggtcaa tatcctcccc tattttcaga
    15301 ttcctgtggg agaatttctg tgctttatga aagacgttct gcccccaagc ctctatgaag
    15361 gtgtggaaaa aatagccaga gaagttttga cgcaaccttc aacaggttta ttgagttttt
    15421 ctgttttatc ggcgttatgg agtttttcaa aatctatgaa tttcttgcaa aaagctttta
    15481 acaaggctta tggcgtcgaa aagagtcgtg gacttatttc ccatcagatg ctaagtctcc
    15541 tagtcagttt tggcttgcag ctcctctttg cgtttgcctt gtttttgatc ttatttggtc
    15601 agatgatttt ggctttactt gctcattatt ggacaaaaga tggcattatc tatcaggctt
    15661 tacaagggtt ggcaggtcct ctgatttacg ccctcctctt tgctatcttg gtcatgcttt
    15721 attattttct tcccaatata tctaacagga aaattagcta taccctacca ggcagtgcct
    15781 ttgttctctt agtgatttta ggtttactaa ctctgttctc tagttatctc aattactatg
    15841 tccatcattt ggtggatgtc cgaattttag gttccgttct tcttgttgtt atgatgtttt
    15901 ggtttatctt gattgctaaa attgttatcc taggcgcggt tatcaatgcc agtatgcaga
    15961 gtttgagaga tccggtcttt aaaaaagact aactatttat ccattacaaa agcgcatagt
    16021 atcaaaactt tatagaacct tgatactatg cgcttttgtg ctttgacagt tttttgtata
    16081 gtttttgtcc aagatttcta aatcatctga ttaaattcct attttcccct atcattgtcc
    16141 ctgttttttc gaaaatggaa ggagtataat aaatctatca ataaaatgat aggaggaact
    16201 ttatgggtca tattttcttt tttctaagtg tctttttggc agggattcta tccttctttt
    16261 ctccttgtat cttacctttg ttaccggtct atacaggagt gttactagat gataaggatg
    16321 gtgctcaggc ttctagcggc aaattttcaa tctcagttac tagtttatta cgaactctgg
    16381 cctttatagc aggaatttcc tttatattta ttttgttggg ctatggagct ggttttttag
    16441 gcgatttgct ttatgcttct tggttccaat atcttactgg ggcaattatt atccttcttg
    16501 gtttgcacca aatggagatt ctacacttta aggggcttta taaggaaaag aggctacaac
    16561 tgcaaggaca ggggcaaaat ggtaagggct atagtcaggc atttttattg ggcttgacct
    16621 ttagttttgc ttggacgcct tgcgtggggc cggttctggg gtctgttttg gccttggcgg
    16681 cttcaggtgg ttcaggagct tggcagggag ctggtctcat gttggtgtat acgctgggct
    16741 tggcgctacc attcttgctt ctagctctga cctctagtta tgttttgaaa catttccgaa
    16801 aacttcatcc ctatctcgga atcctcaaaa aagtgggtgg ttttctcatt attgtgatgg
    16861 gactcttggt tctgtttgga aatgcttcaa ttttaagtca attatttgaa taaaatggaa
    16921 aggaatatca atatgaaaaa atggcaaaca tgtgttcttg gagcaggttc gctcctttgt
    16981 ttgacggctt gttcaggcaa gtccgtgact agtgaacacc aaacgaaaga tgaaatgaag
    17041 acggagcaga cagctagtaa aacaagcgca gctaaaggga aagaggtggc tgattttgaa
    17101 ttgatgggag tagatggcaa gacctaccgt ttatctgatt acaagggcaa gaaagtctat
    17161 ctcaaattct gggcttcttg gtgttccatc tgtctggcta gtcttccaga tacggatgag
    17221 attgctaaag aagctggtga tgactatgtg gtcttgacag tagtgtcacc aggacataag
    17281 ggagagcaat ctgaagcgga ctttaagaat tggtataagg gattggatta taaaaatctc
    17341 ccagtcctag ttgacccatc aggcaaactt ttggaaactt atggtgtccg ttcttaccca
    17401 acccaagcct ttatagacaa agaaggcaag ctggtcaaaa cacatccagg attcatggaa
    17461 aaagatgcaa ttttgcaaac tttgaaggaa ttagcctagg aggtgtctta tgaatgataa
    17521 gttaaaaatc ttcttgttgc taggagtatt ttttctagcc ataaccggtt tctatgttct
    17581 attgatacga aatgcagggc agacagatgc ctcgcaaatt gaaaaggcgg cagttagcca
    17641 aggaggaaaa gcagtgaaaa aaacagaaat tagtgaagac gcagacttgc acgaaattta
    17701 tctagctgga ggttgtttct ggggagtgga ggaatatttc tcacgcgttc ccggggtgac
    17761 ggatgccgtt tcaggctatg caaatggtag aggagaaaca accaagtacg aattaattaa
    17821 ccaaacaggt catgcagaaa ccgtccatgt cacctatgat gccaagcaaa tttctctcaa
    17881 ggaaatcctg cttcactatt tccgcattat caatccaacc agcaaaaata aacaaggaaa
    17941 tgatgtgggg acccagtacc gtactggtgt ttattacaca gatgacaagg atttggaagt
    18001 gattaaccaa gtctttgatg aggtggctaa gaaatacgat caacctctag cagttgaaaa
    18061 ggaaaacttg aagaattttg tggtggctga ggattaccat caagactatc tcaagaaaaa
    18121 tccaaatggc tactgccata tcaatgttaa tcaggcggcc tatcctgtca ttgatgccag
    18181 caaatatcca aaaccaagtg atgaggaatt gaaaaagacc ctgtcacctg aggagtatgc
    18241 agttacccag gaaaatcaaa cagaacgagc tttctcaaac cgttactggg ataaatttga
    18301 atccggtatc tatgtggata tagcaactgg ggaacctctc ttttcatcaa aagacaaatt
    18361 tgagtctggt tgtggctggc ctagttttac ccaacccatc agtccagatg ttgtcaccta
    18421 caaggaagat aagtcctaca atatgacgcg tatggaagtg cggagccgag taggagattc
    18481 tcaccttggg catgtcttta cggatggtcc acaggacaag ggcggcttac gttactgtat
    18541 caatagcctc tctatccgct ttattcccaa agaccaaatg gaagaaaaag gctacgctta
    18601 tttactagat tatgttgatt aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
    18661 cttaccgtat gtagggttac tgacttcgtc agttttatct acaacctcaa agctatactt
    18721 tgagctgact tcgtcagttt tatctacaac ctcaaagcgg tactttgaga aacctgtggc
    18781 tatcttccta gtttactttt tgatttttat tgagtataag aggtctttcc taagcagtta
    18841 gaggagaatt ttgctatact gatactagta agtggtaaag gagcagagca tgacctacac
    18901 aatcttaatc gtagaagatg aatatctggt aagacaaggt ttgactaaac tggtcaatgt
    18961 agcagcctac gatatggaaa tcatcggtca ggctgaaaat ggaaggcagg cttgggaatt
    19021 gatccaaaag caggtgccag atatcatttt aaccgatatc aacatgcctc atctaaatgg
    19081 catccagttg gccagtctgg tacgagaaac ctatcctcag gttcatttgg tctttttaac
    19141 aggttacgat gattttgatt atgccttgtc tgctgtcaaa ctaggtgtgg acgactacct
    19201 gctcaaaccc ttttctcgtc aggatattga ggaaatgttg gggaaaatca aacaaaaact
    19261 agacaaggaa gagaaagaag agcagttaca agatttattg actaataggt ttgaaggaaa
    19321 catggcccag aaaatccagt ctcatctggc tgatagccaa tttagtttaa agtctttagc
    19381 cagtgactta ggttttagtc cgacctatct gagttccttg attaagaaag agttgggctt
    19441 gccttttcag gattatctgg tgagagaacg tgttaaacaa gccaagctct tgcttttaac
    19501 tacagatctg aagatttatg agatcgcaga gaaggttggt tttgaagata tgaactattt
    19561 tacccaacgt tttaagcaga ttgcaggtgt gacacctcgt cagtttaaga agggagaaga
    19621 ccgatgaagc gttcttctct tttagttaga atggttattt ccatctttct ggtctttctc
    19681 attctcctag ctctggttgg aactttttac tatcaatcaa gttcttcagc cattgaggcc
    19741 accattgagg gcaacagcca aacgaccatc agccagacta gccactttat tcagtcttat
    19801 atcaaaaaac tagaaaccac ctcgactggt ttgacccagc agacggatgt tctggcctat
    19861 gctgagaatc ccagtcaaga caaggtcgag ggaatccgag atttgttttt gaccatcttg
    19921 aagtcagata aggacttgaa aactgttgtg ctggtgacca aatctggtca ggtcatttct
    19981 acagatgaca gtgtgcagat gaaaacttcc tctgatatga tggctgagga ttggtaccaa
    20041 aaggccattc atcagggagc tatgcctgtt ttgactccag ctcgtaaatc agatagtcag
    20101 tgggtcattt ctgtcactca agaacttgtt gatgcaaagg gagccaatct tggtgtgctt
    20161 cgtttggata tttcttatga aactctggaa gcctatctca atcaactcca gttggggcag
    20221 cagggctttg ccttcattat caatgaaaac catgaatttg tctaccatcc tcaacacaca
    20281 gtttatagtt cgtctagcaa aatggaggct atgaaaccct acatcgatac aggtcagggt
    20341 tatactcctg atcacaaatc ctacgtcagt caagagaaga ttgcaggaac tgattggacg
    20401 gtgcttggcg tgtcatcatt ggaaaagtta gaccaggttc ggagtcagct cttgtggacc
    20461 ttgcttgggg ccagtgtcac atctcttctt gtctgtctct gcttagtgtg gttcagtctt
    20521 aaacgctgga ttgctccttt gaaggatttg agagaaacca tgttggaaat tgcttctggt
    20581 gctcaaaatc ttcgtgccaa ggaagttggt gcctatgaac tgagagaagt aactcgccaa
    20641 tttaatgcta tgttggatca gattgatcag ttgatggtag ctattcgtag ccaggaagaa
    20701 acgacccgtc agtaccaact tcaagccctt tcgagccaga ttaatccaca tttcctctat
    20761 aacactttgg acaccatcat ctggatggct gaatttcatg atagtcagcg agtggtgcag
    20821 gtgaccaagt ccttggcaac ctatttccgc ttggcgctca atcaaggcaa ggacttgatt
    20881 tgtctctctg acgaaatcaa tcatgtccgc cagtatctct ttatccagaa acaacgctat
    20941 ggagataagc tggaatacga aattaatgaa aatgttgcct ttgataattt agtcttaccc
    21001 aagctggtcc tacaacccct tgtagaaaat gctctttacc atggcattaa ggaaaaggaa
    21061 ggtcagggcc atattaaact ttctgtccag aaacaggatt cgggattggt catccgtatt
    21121 gaggatgatg gcgttggctt ccaagatgct ggtgatagta gtcaaagtca actcaaacgt
    21181 gggggagttg gtcttcaaaa tgtcgatcaa cggctcaaac ttcattttgg agccaattac
    21241 catatgaaga ttgattctag accccaaaaa gggacgaaag ttgaaatata tataaataga
    21301 atagaaacta gctaactccc agtcaattct gggagtttta tgcgtaattg ggcaaacttt
    21361 ctaggttgtc tatcttgcta aaaacaagaa aaaacggtat gatagaagag tgacaacaga
    21421 ggtaacagag atgaacgaga cacagattca aagagaaaca cgtcaggtag tggaagatgt
    21481 tttagaaaag accaatttga agcagggggc cctttttgtt ttaggtctat cttctagtga
    21541 ggtgctaggt ggtcatattg ggaaagaatc cagtcaagag attggtgagc tgatagtgga
    21601 gactatcttg gatattctag gtagtagagg tatccattta gcagttcaag gttgtgagca
    21661 tgtcaatcgg gctctcgttg ttgaacgtca ggtggcagag cagtttggtc tggaaattgt
    21721 cagtgtccat cctactcttc atgcaggagg ttcggggcag ttggcagcct ttaagtttat
    21781 gcaggatcca gttgaggttg aatttatcaa ggctcatgct ggattggata tcggagacac
    21841 tgcaattggc atgcatgtca agcatgttca ggttccgatt cgccctattt tgagagagat
    21901 tggtcatgcc catgtaacgg cactggctag tcgtccaaaa ttaatcggag gtgcgcgtgc
    21961 gcactatccg caagatgcta ttagaaagac ctgagaatga ataaaggaga taaaagtgtt
    22021 taaaaaagac cgtttttcaa ttcgtaagat taagggagtt gtaggctctg tatttcttgg
    22081 aagccttttg atggctcctt ctgtagtgga cgcagccacc tatcactatg taaataaaga
    22141 gattatttca caagaagcta aagatttaat tcagacagga aagcctgaca ggaatgaagt
    22201 tgtatatggt ttggtgtatc aaaaagatca gttgcctcaa acagggacag aagcatctgt
    22261 tttgacagct tttggttatc tatcaggtga catcctcaaa acgcttggct tggacactgt
    22321 tttagaagaa acctcagcaa aacctggaga ggtgactgta gtcgaagttg agactcctca
    22381 atcaacaaca aatcaggagc aagctaggac agaaaaccaa gtagtagaga cagaggaagc
    22441 tccaaaagaa gaagcaccta aaacagaaga aagtccaaag gaagaaccaa aatcggagat
    22501 aaaacctact gacgacaccc ttcctaaagt agaagagggg aaagaagatt cagcagaacc
    22561 agctccagtt gaagaagtag gtggagaagt tgagtcaaaa ccagaggaaa aagtagcagt
    22621 taagccagaa agtcaaccat cagacaaacc aacagaggaa ccaaaagttg aacaagtagg
    22681 tgaaccagtc gagccaagtg aagacgaaca ggcaccaacg gcaccagttg agccagaaaa
    22741 gcaaccagaa gctcctgaag aagagaaggc tgtagaggaa acaccgaaac cagaagataa
    22801 aataaagggt attggtacta aagaaccagt tgataaaagt gagttaaata atcaaattga
    22861 taaagctagt tcagtttctc ctactgatta ttctacagca agttacaatg atcttggacc
    22921 tgttttagaa actgcaaaag gtgtctatgc ttcagagcct gtaaaacagc ctgaggtaaa
    22981 tagtgagaca aataaactta aaacggctat tgacgctcta aacgttgata aaactgaatt
    23041 aaacaatacg attgcagatg caaaaacaaa ggtaaaagaa cattacagtg atagaagttg
    23101 gcaaaacctc caaactgggg ttacagaagc tgaaaaagtt gcagctaata cagatgctaa
    23161 acaaagtgaa gtaaattctg agactgcaag tcttaaaaca gcaatttcta gattaaatac
    23221 agataaagtt gaactcgaaa atcaacttaa gattgcacag ggaaaaactg aaactgattt
    23281 cagcatggaa tcttggactg ttttgagtac agctaaaaat aaagctcagg aagtaaaaga
    23341 taacggaaca gcgacccaag aacaaataaa cgaagcagag aaaagtttga aaacagccct
    23401 tgctgactta tctgttgaca aaacagcttt gggaagcgct atcgatacag ctactaagaa
    23461 aaataaagag aattatacaa atcaaacatg ggcagagttg gaaacagcgc ttacagcagc
    23521 aaaatctgta aatactaacg agtctaaaca aagcgacgtt aacgaagctg ctgaaaaatt
    23581 aactgcaact atggaaaaat tggttgaatt gtctgaaaag ccacgtttga ctctatctat
    23641 tgaaaagaga gacatagatc gaaaagctac ggtcacgtat actttggaaa atccagcaaa
    23701 tactcaaata aaatcaataa ctgcaactct gaaaaaagga gaggaagttg ttaaggattt
    23761 tgttttaaca gaagaaaatc ttaaaactaa tcatttaact gctttgtttg aaaagctaga
    23821 ctactacaaa gaatacactc tatctacgga tatggtatat aacagaggaa atgatgatga
    23881 gacggagagt atttcagaag aactaattca gctgaatctt aagaaactag aattgaaaga
    23941 tatccagaca gtctctctga tgaaatttga aaatggtcaa gaaagtcagg ttacacactt
    24001 gtctgataaa ccgaccgatt tgagtaaatt atacttaaaa gttacatcat caacgtctaa
    24061 ggatgctgtg ttagccgtta gctctataga agaagagatt attgaaaata aaaaaatctt
    24121 taaaattcat gcagatacac cagaattagt agttcgtaag aaagatggca gtctttctaa
    24181 gggatttgac tattacatgg agagagttat ccctcatgat ggtgatattt attatgattt
    24241 taaagacttg atttcagcta tgacatctaa tcctacaggt acgtttattt tagggagaga
    24301 tatttcctcg cgtaatgtaa aaccggatgg aaatggaaaa tcttatatca aaggtgaatt
    24361 taaaggaaaa ctacttggta caaatgataa tgttagacac tcgatctttg acttagaata
    24421 tccattgttt gatactataa aatcaggagt cgtgaaagat attgatttca agcatgtcaa
    24481 tatggtattc ccagatagca atcaaggaga taacgtagca acaattgcaa gagtaatcaa
    24541 agataagaca aaaattgaga atgttaatgt tgaaggttat ctcgagggtc gagatcatgt
    24601 agctggttta gttaataatc ttgaaggtaa ttccgaaatt gaaaatgtat cctttacagg
    24661 aaagattaag tctaaaggtg gtaattctat tacagcaggt attgcaggta gaaatatctt
    24721 atctcgtgtt aaacgtgcct atgtaaatgc agatatagag gtacatcgat catctaattc
    24781 tagcatgctt gttgcagtaa atggaataaa cgcagatgca agtggtggtt ggggaacctg
    24841 gggtagactt actgagtcag ttgctaaagg aacattagaa accaagcaag gagggcaagc
    24901 tggaggagcc tcttctacag tctggcctta tggagcaatt gataatgttg tcagctatgc
    24961 aaaagtgacc aaagggaaag aattatttgg atcagatggt gatttaaatt atgattggtt
    25021 tatgaaaaaa attagtaata tttttggtgt tcaaggcatc agctcaggcg attcgggtag
    25081 tgattctaaa tttacgcgta tttctgagga agaagcaaat caaaaagttg cttcttataa
    25141 tattacagct cccaacttga tgagtgactc aagtcttttg gtagacagac tcaatgaaag
    25201 ttggaaaaat actgatcaat ttgaaagtat acaggattat caagcccaaa accaattaat
    25261 ttaccagaac ttgactaaat ttactcctta ttacaataaa gaatttattg ttcatgaagg
    25321 aaatgctctc actcctgaac aggaaatatt gaagactaag aaaatcaaat cgattgtagg
    25381 gttgaaagga acagagttcg ttgtagatgg cagtgatatt gatactatca tgcttcattt
    25441 tgaggatggt agtcagaagc gatataaggt aacctctact ggtaagtttt cgataactaa
    25501 tttaccggaa tatcaggttg aagatttaaa tgtgatgtat acatcagaac atatagtaca
    25561 tccactagat tcgtcactga taaataacct tgttgaagaa ttgaaaaaag tagaattgta
    25621 tacagagtct acttaccaag ttctaggtat tgataaagat aatgctaaca aattgaatag
    25681 aactaagaga ttattcttag atgagtcatt agatgctgtt aaaacacaat tgcctacctt
    25741 tgtaaaaaca atgtttgaaa atgagtggtt gcacatcaat ggagaatcta gtggagctgt
    25801 cgctgcttta cgacaaaaaa tcatggataa taagacggct atattgcttg ctttgactta
    25861 tatcaatcgt tattatgatg ttaaattctc agactataat attaaaaaac tgatgctctt
    25921 taaaccaact ttccatggtg aaaagattga tctattagat cgattaatcc gactaggatc
    25981 atctggagaa aatagattaa aaggttcaga aaatgcagaa acgttcaagc agttatttgc
    26041 tagcgaaacg aagcagaagg atttggtgac ttatttagac tacaatcgct ctttgttgac
    26101 taattatcaa acaacagggg aatggtttaa agaaacgact aaggattata ttcaatttga
    26161 ggaacgtcca tccttagtag aggagattaa agatgccaaa tatcgagttt acgataattt
    26221 aaccgcaccg tattatcaag gatatatcct acctctctta acgttgaaga atactcattt
    26281 agcaatttta tcgaactata gtactatgac atttgtcagc cgtgaaaaac gccctaattg
    26341 gaaaaatgaa gattttgata agtgggtgaa atatgtagca actgctcata gaaaccatgt
    26401 tgatacttgg tataagattt tacctgataa tatcaaaggt aaaatggtta aagagaatgt
    26461 tactgctgtt tgggaaggat tgagcatccc aggaagtgaa tgggtagacc agaatgctgt
    26521 agatagaaaa ggaagagatt acgccccagc tcgagaattc tttaatcttg taggtggtcc
    26581 tatgggaggt tggtatgctt accatggtta tggtgcgcat gctggaggaa gaaatcgggt
    26641 taattatgag gtgtttgatg tattaagtga gtatggtata tcggtcttta ctcatgaatt
    26701 gactcacgtt aatgatactt ggatttactt aggagggtat ggtagacgtg aaaatatggg
    26761 acctgaagct tatgctcagg gattattcca gtctcctgtt ccaggtcagc cgggttgggg
    26821 ggcgcttggt ctcaatatgg catttgaaag aaaaaatgat ggtgatctaa tttataatgc
    26881 ctccccaact caatttgaaa ataggaaaga gttagatagt tatatgaaaa attataacga
    26941 tactctcatg atggtggact acttggaagg agatgcagtt atctctaaag gtaaagaagc
    27001 tattactaaa tggtttaaaa aagttgaacc taaggttgtt agtcaaacag ctcagtatga
    27061 tacggttcgt cagttgactg ctgaagagaa agaaaaactt tctgtttcta gtgttgatga
    27121 tttagttgat caaggattaa tgtcagacag agctgtagga aataatacct ataatccagc
    27181 tgattttgaa acaagttata ttgcgataga ttatatgact ggtatttatg gtggaggaaa
    27241 gaatagtgta ggttcaccag gtgccttgat gtttaaacat aatactttcc gtatgtgggg
    27301 ctactatggt tttgaagaag gtgttctagg atatgcttct aataaattta agcaagcatc
    27361 tagagatgaa ggtcatgctg gacttagtga taactttatt atttctaaga tttccaaagg
    27421 ggaattttta actatggaag catttaaaaa gggatatttt aagaaagtag tagaagagtt
    27481 aaaaactaaa ggtattcgtc ctgttactat caatcaaaaa acatatagca cctttgaaga
    27541 attgcaagaa ggatttaagc aggctgttga aagagatttg aagaaaaatc agttagatga
    27601 gagggaaaca agaaacttca aattccaagt tttcagacaa cttcttcaac aaacagatag
    27661 ctttaaaact tctatcttta gataatcgaa atctctcatc tgctttgcgg atgagggttt
    27721 tcttatttta tgctatactt aaagtatgca tagaaaaaca gtgattgatt ttaggacttt
    27781 gggggagaga tataccttta cccagcctat taaagagttg aaaacgagaa atgtagaaga
    27841 agtggcagat ttgctggcac aagtggaaag ctaccaagag caaggttatt atgtggtggg
    27901 gtatgtcagc tacgaggctg cacctgcttt tgaggaaaaa ttagcagttc acaaggatcc
    27961 cctactggga gagtacctgc tttattttac tgttcatgat agcgtagaaa cttcccctat
    28021 tcctctgact tatgaggata ttgatttgcc ctcaaattgg caggaagtaa cgtctgcagc
    28081 agactatgaa aaggccattg cccaaattca ccatcattta cggcagggag atacctatca
    28141 ggtcaactac accgtccaac ttaagcaaaa gttaaatgcc aatccttttg ccatctacaa
    28201 tcgtatggtg gtagagcagg aggcgggcta caatgcctat gtggaacatg acgagatggc
    28261 agtgatttcc atgagcccag agctcttttt tgagcaaaat gatcgcgagt tgacaacacg
    28321 accaatgaag gggacgactc agcgtggggt aactgaccaa gaagatcttg aacaggccag
    28381 ttggttggaa caggatccca aaaatcgctc tgaaaatatg atgattgtgg acctcttgcg
    28441 caatgatatg aaccgtattt ctgaagttgg gagcgagcac gtggagcgtc tgtgtcaggt
    28501 agagcagtat tcaactgttt ggcagatgac ttcgaccatc aagagtcagt tgcgagagga
    28561 tgtggacctt gttgaaatct tccactcact ctttccttgt ggttccataa cgggtgcacc
    28621 gaaaattgcg acaatggaga ttatcaagga cttggagcct caaccgcgtg gagtctactg
    28681 tggaacgatt ggtttcttgc ttccaaatgg acgacggatt tttaatgtgg ccattcgtac
    28741 cattcaactt cacaaaggtc aagccattta tggagttggc ggagggatta cttgggatag
    28801 cacttgggaa tctgaatacc gtgaagttca tcaaaaggca gcagttctct atcgtaaaca
    28861 agctcgcttc caattaatta caactggaaa aatcagccaa aaacaactga tgtttgaaga
    28921 acaacatctg gaaaggctga gaaaagctag tcggtatttt gcttatcctt ttgacccaga
    28981 aattttgaga caaaagattg aagcagagtg tcagacttgt gatgctaatc aagattaccg
    29041 cttgcgaatt tctctcagca agtctggaga gatagaattc agtcgtcaaa tcttaacacc
    29101 tcttagtcca agtttttgtc aggccaaact ttgtctgcaa gaagcgaatt tgaatcaatc
    29161 ctttacctat tttaaaacga cttacagacc gcatttgagc atagaggaac aagagaagat
    29221 ttaccataat ggaaaaggag aacttcttga aacctctatc ggaaatttgg ttctgaaaat
    29281 cgatgggaaa ctctacacac cgcctatccg acttggaatc ttgccaggaa tttatcacca
    29341 gtatttacta gaaacaggac aggtagaaga gaaagtcttg accttggcag attttgccca
    29401 agcagaagct atttacggct gtaatgcagt gagaggcttg tatgagttgc aagtgatagg
    29461 attgtgagtt ttagctatta atagttttta atatatatta aaata
//
LOCUS       DAVLSL010000008        29460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLSL010000008 DAVLSL010000000
VERSION     DAVLSL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.008"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            359..1522
                     /locus_tag="VXN30_000275"
     CDS             359..1522
                     /locus_tag="VXN30_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1356953.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1519..2196
                     /locus_tag="VXN30_000276"
     CDS             1519..2196
                     /locus_tag="VXN30_000276"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1356954.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2186..3040
                     /locus_tag="VXN30_000277"
     CDS             2186..3040
                     /locus_tag="VXN30_000277"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1356955.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALENVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3059..4126
                     /locus_tag="VXN30_000278"
     CDS             3059..4126
                     /locus_tag="VXN30_000278"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1356956.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKTRGNTLKLVLVS
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4136..5302
                     /gene="aroC"
                     /locus_tag="VXN30_000279"
     CDS             4136..5302
                     /gene="aroC"
                     /locus_tag="VXN30_000279"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1356957.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5312..6415
                     /locus_tag="VXN30_000280"
     CDS             5312..6415
                     /locus_tag="VXN30_000280"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1356958.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6426..6764
                     /locus_tag="VXN30_000281"
     CDS             6426..6764
                     /locus_tag="VXN30_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1356959.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6856..8139
                     /gene="aroA"
                     /locus_tag="VXN30_000282"
     CDS             6856..8139
                     /gene="aroA"
                     /locus_tag="VXN30_000282"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1356960.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8132..8608
                     /locus_tag="VXN30_000283"
     CDS             8132..8608
                     /locus_tag="VXN30_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1356961.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIENRLGMSIANFFA
                     EKGETAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLRADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8605..9453
                     /gene="pheA"
                     /locus_tag="VXN30_000284"
     CDS             8605..9453
                     /gene="pheA"
                     /locus_tag="VXN30_000284"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1356962.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9450..10724
                     /gene="psr"
                     /locus_tag="VXN30_000285"
     CDS             9450..10724
                     /gene="psr"
                     /locus_tag="VXN30_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1356963.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRLQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11003..11848
                     /locus_tag="VXN30_000286"
     CDS             11003..11848
                     /locus_tag="VXN30_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1356964.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EYNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11838..12941
                     /locus_tag="VXN30_000287"
     CDS             11838..12941
                     /locus_tag="VXN30_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1356965.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEEFLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12943..13929
                     /locus_tag="VXN30_000288"
     CDS             12943..13929
                     /locus_tag="VXN30_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1356966.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAYFFILDKMLLDDQYKQFKAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13945..15378
                     /locus_tag="VXN30_000289"
     CDS             13945..15378
                     /locus_tag="VXN30_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1356967.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15407..16441
                     /locus_tag="VXN30_000290"
     CDS             15407..16441
                     /locus_tag="VXN30_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1356968.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16641..17378
                     /gene="mecA"
                     /locus_tag="VXN30_000291"
     CDS             16641..17378
                     /gene="mecA"
                     /locus_tag="VXN30_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1356969.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LEELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17529..18815
                     /locus_tag="VXN30_000292"
     CDS             17529..18815
                     /locus_tag="VXN30_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1356970.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18817..19686
                     /locus_tag="VXN30_000293"
     CDS             18817..19686
                     /locus_tag="VXN30_000293"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1356971.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19756..19836
                     /locus_tag="VXN30_000294"
                     /pseudo
     CDS             19756..19836
                     /locus_tag="VXN30_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            20026..20964
                     /gene="msrB"
                     /locus_tag="VXN30_000295"
     CDS             20026..20964
                     /gene="msrB"
                     /locus_tag="VXN30_000295"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1356972.1"
                     /translation="MTEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21022..22746
                     /locus_tag="VXN30_000296"
     CDS             21022..22746
                     /locus_tag="VXN30_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356973.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYAKLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22748..24496
                     /locus_tag="VXN30_000297"
     CDS             22748..24496
                     /locus_tag="VXN30_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1356974.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGRVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEAIREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24888..26147
                     /locus_tag="VXN30_000298"
     CDS             24888..26147
                     /locus_tag="VXN30_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1356975.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSNDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26223..26358
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26397..26897
                     /locus_tag="VXN30_000299"
     CDS             26397..26897
                     /locus_tag="VXN30_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1356976.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26973..27341
                     /gene="rplL"
                     /locus_tag="VXN30_000300"
     CDS             26973..27341
                     /gene="rplL"
                     /locus_tag="VXN30_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1356977.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27528..27698
                     /locus_tag="VXN30_000301"
     CDS             27528..27698
                     /locus_tag="VXN30_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356978.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27698..27949
                     /locus_tag="VXN30_000302"
                     /pseudo
     CDS             27698..27949
                     /locus_tag="VXN30_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27936..28283
                     /locus_tag="VXN30_000303"
                     /pseudo
     CDS             27936..28283
                     /locus_tag="VXN30_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28276..28656
                     /locus_tag="VXN30_000304"
     CDS             28276..28656
                     /locus_tag="VXN30_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1356979.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28669..28812
                     /locus_tag="VXN30_000305"
     CDS             28669..28812
                     /locus_tag="VXN30_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356980.1"
                     /translation="MAERFWENLSIILAERNISCIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            28905..>29460
                     /locus_tag="VXN30_000306"
     CDS             28905..>29460
                     /locus_tag="VXN30_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1356981.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVW"
BASE COUNT         8750 a         5437 c         6614 g         8659 t
ORIGIN      
        1 gtaacaaaag gcttctctat aaaatcaaat tttccagata cctactgaag ctgatttatt
       61 tcttcatctt tcttctacta cctttgtcac taaatttgac agacacatat tgctgataca
      121 acctctctac ctataatctc acggttaacc tgaggctagt ttcatagttt gctatttgat
      181 tttcgttgat aaaaagaaat gctcctccaa gacggaagag cattttttat tgtgattctt
      241 acacatttat ttcctagttt tgcaggaagt ctagtatacc attttttaaa agaagagtaa
      301 aaaagcaagg gtagtttctt ttttgagaat tgtctaaaag tttgatataa tggtagttat
      361 gaatagaata agagtcagca aaagggttga aaagaagctt gctaaggggc tagttttact
      421 agaagccagt gatcttgaga atgtcaatct taaggatcag gaagtagagg tgcagggtca
      481 ggaaggaaac tttcttggga ctgcctacct ttctcagcaa aacaagggct tgggctggtt
      541 tgtcagcaaa gacaaggtgg ccttcaatca aactttcttt gaaacgttgt ttagaaaagc
      601 caaagaaaag agaaacgcct actatcaaga tgatttgaca actgcctttc gtctctttaa
      661 tcaagaggga gatggctttg ggggtctgac agtggacctt tatggcgact acgccgtctt
      721 ttcttggtat aactcttatg tttatcagat tcgtcagact atatcagaag cctttagaca
      781 ggttttccct gaggttttag gggcttatga gaaaatccgc tttaagggtt ttgactatga
      841 atctgcccat gtttatggtc aagaagcacc tgactttttc actgttttag aaaatggtgt
      901 cctgtatcaa gtctttatga atgatggctt gatgacagga attttcctag accagcatga
      961 ggttcgcggt agtttagttg acggcttggc tatgggtaaa tccttgctca atatgttttc
     1021 ctacacagcg gctttttcag tagctgcggc catgggagga gctagccaga caacttctgt
     1081 tgatctagcc aaacgttcac gagaattgtc tcaagcgcat tttcaggcaa atgggctcag
     1141 cacagacgag catcgtttta tagtcatgga tgtctttgag tatttcaaat atgccaaacg
     1201 caaagacttg acctacgatg tgattgtcct agatccgcct agctttgctc ggaataaaaa
     1261 acaaactttc tctgtggcca aggattatca caagttgatt tcccagagtc ttgagatttt
     1321 aaatccggga gggattatca ttgccagtac caatgctgcc aatgtttccc gtcagaaatt
     1381 tacagaacaa attgataaag gctttgcagg aagaagttac cagattttaa acaaatatgg
     1441 tcttccagca gattttgcct ataataaaaa agatgaaagt agtaattacc tcaaggtgat
     1501 tagtatgaag gttagtaaat gaaattaatc gtttcagtaa tgccaagaag tttagaggag
     1561 gctcaggctc tggatgccac gaggtacctg gatgccgaca tcattgaatg gcgtgccgac
     1621 tatctgccta aagaagcgat tttgcaggta gctccagcca tttttgaaaa attcgcaggc
     1681 cgtgagttgg ttttcacgct acgaactcgc tccgaaggtg gagaaatcga cctttctcca
     1741 gaagagtata tccatctaat caaggaagtt gcgcaattct atcaaccaga ctatattgat
     1801 tttgagtact atagctacaa ggatgttttt gaggaaatgc tggacttccc aaatcttgtt
     1861 ttgagttacc ataatttcca agaaacacct gagaacatga tggaaatctt gtcagagttg
     1921 acgatcctaa atccaaaact tgttaaggtt gcggtgatgg ctcacacgga gcaggatgtc
     1981 ttagacttga tgaactatac acgaggcttt aaaaccctca atcctgaaca ggaatatgtg
     2041 accatttcta tgggtaaggt gggcaaggtc tctcgtatca ctgcggatgt gactggttcg
     2101 agttggtctt ttgccagtct ggatgaggtt agtgcccctg ggcagatttc tctagctagc
     2161 atgaaaaaaa tcagggagat tttggatgaa gcttgatggc tatacacgtt tagctgcagt
     2221 tgttgccaat cctattaagc attctatttc tcccttcatc cacaatagag cctttgaggc
     2281 gacagctacc aacggtgctt atgtggcttg ggagattgaa gcgagtgact tggcagaaac
     2341 agtggccaat attcgtcgct accagatgtt tggcatcaat ctgtccatgc cctataagga
     2401 gcaggtgatt ccttatttgg ataagctgag cgatgaagcg cgcttgattg gtgcggttaa
     2461 tacggttgtc aatgagaatg gcaatttaat tggatataat acagatggca agggattttt
     2521 taagtgcttg ccttctttta caatttcagg taaaaagatg accctgctgg gtgcaggtgg
     2581 tgcggctaaa tcaatcttgg cacaggctat tttggatggc gtcagtcaga tttcggtctt
     2641 tgttcgttcc gtttctatgg aaaaaacaag accttaccta gacaagttac aggagcagac
     2701 aggttttaaa gtggatttgt gtgctttaga gaatgtttct gaactgcaag caaggattgc
     2761 cgagtcggat ttgctagtta atgccaccag tgtgggcatg gatggccaat cctctcctgt
     2821 tcctgaaaac atagtcttac cagaaactct cttagttgca gatatcatat accaaccctt
     2881 tgaaacaccg tttttgaaat gggctagaag tcagggcaat ccagcagtca atggtctggg
     2941 aatgttgctc tatcaagctg cagaagcttt tcaactgtgg acaggcaagg aaatgccgac
     3001 agaagagatt tggcagtctt taacagaaaa ataccaataa agaaagggag actctcctat
     3061 gaaaatcaga atcgatattc ctcatcatcc ttatgatatt cagattgaaa aaggttgtat
     3121 ggcccaggct ggtcagtggt tgcgagaact ctggcaaccg caaaaggtag tcattgtgac
     3181 agataaccat gtagcctctc tctatgcaga gaaggtcaag ctcagcctag aagatgctgg
     3241 ttttcaggta gctgtttttg atttcttaga aggtgaagaa agaaagaatt taactactgt
     3301 tcagaaagtc tatgaatttc tagtcaagca aggtctgact cgtagcgatg gaatcgttgc
     3361 tcttggtggt ggcgttgttg gggacctggc tggatttgta gcctctacct atatgcgggg
     3421 tattcacttt gttcagattc cgactagttt gacagctcag gttgattctt ctatcggtgg
     3481 aaagacaggc gttaacacac catttgctaa aaatatggtg gggacctttg cccaaccaga
     3541 tggggttctg attgatccac ttgttcttga aaccctcgga aaaagagagt tgattgaagg
     3601 gatgggtgaa gttatcaagt atggcttgat tgaggatcca gaactgtggg ctctcttgac
     3661 ggaactgaat ggttctgttg agagcatttt ggaacatgca gagaccttga ttgaacattc
     3721 ttgtcaggtg aagcgcaaga tggtggttga agatgagttg gacaatggta ttcgtcttta
     3781 cctcaatttt ggccacacta ttggccatgc catcgaagcg actgccggtt atggcaaggt
     3841 catgcatgga gaggctgttg ccatgggaat ggtacagatt tccaaggttg ctgaggaaaa
     3901 aggcctcatg ccagctggca ttacccaatc tatcacagag atgtgtcaga aattcggctt
     3961 gcctgttgac tatgaaaatt gggaagttga caagctttat caggctctta ctcatgacaa
     4021 gaaaacgcgt ggtaacacct tgaaattggt cttggtgtca gagcttggtt cagcgatcat
     4081 tcacccagtt tctctggaag agatgaaaga ctacttggta aaataaggag agtgtatgag
     4141 atatttaact gcaggagaat cacacggccc ccgtctaaca gctattattg agggaattcc
     4201 agctggactt ccattgacag ctgaggatat caatgaggac cttagacgcc gtcagggtgg
     4261 ctacggtcgt ggtggtcgta tgaagattga gagtgaccag gttgtcttta cttcgggcgt
     4321 tcgccacggg aagacgacag gggcgcctat tactatggat gtcatcaata aggaccacca
     4381 gaaatggctg gacatcatgt ctgcggagga cattgaagac cgccttaaaa gcaagcggaa
     4441 aattactcat cctcgcccag gtcatgccga tttggttggg gggattaagt accgttttga
     4501 tgatttgcga aattctttgg agcgttcatc agctcgtgaa accaccatgc gggtggcagt
     4561 tggtgcagta gccaaacgcc tcttggctga gctggatatg gagattgcca accatgtcgt
     4621 ggtctttggt ggcaaggaaa tcgatgttcc tgaaaatctg acagtcgctg aaattaagca
     4681 aagagctgcc cagtctgaag tttctattgt taaccaagaa cgagaacagg aaatcaagga
     4741 ctatattgac caaatcaaac gtgatggtga taccatcggt ggggttgtgg agacagtcgt
     4801 cggaggcgtt ccagttggtc ttggttccta tgtccaatgg gatagaaaat tggatgcaag
     4861 attggctcaa gctgttgtct ctatcaatgc ctttaaaggg gtggaatttg gtcttggctt
     4921 tgaggctggt tatcgtaaag gcagccaagt tatggatgaa attctctggt ctaaagaaga
     4981 cggttatact cgccgtacca ataatctagg tggttttgaa ggtggtatga ctaatgggca
     5041 acccatcgtt gttcgtgggg tcatgaaacc cattcctact ctttataaac ctcttatgag
     5101 tgtggatatc gaaacccacg aaccttacaa ggcaaccgtg gagagaagtg atccgactgc
     5161 tcttccagct gcaggaatgg tcatggaagc agttgtagca acggttctgg cgcaagaaat
     5221 cctcgaaaaa ttctcatcag ataatcttga ggaactaaaa gaagcggtag ccaaacaccg
     5281 agactataca aagaactatt aaggagttcc tatggcaaaa acaatctata tcgcaggtct
     5341 tgggttgatt ggagcctcta tggcacttgg tatcaaacgc gatcatccag attatgaaat
     5401 tttaggttat aatcgtagtc aagcttcgag agatatcgcc ttgaaagaag gcatgattga
     5461 ccgtgcaacg gatgattttg ctagttttgc tcctttggca gatgtcatta tcctcagctt
     5521 gccaatcaaa caaactattg ctttcattaa ggagttggcc aatttggatt tgcgagaagg
     5581 cgttattatt tcagatgctg gttcgaccaa gtcaaccatt gtggatgcgg cggagcagta
     5641 tttggctggc aagtctgttc gctttgtcgg ggcccatccc atggctggta gtcacaagac
     5701 aggggctgct tcggcagatg tcaatctttt tgaaaatgcc tattatatct ttacaccttc
     5761 aagcctgaca agtcaggaca cgcttaagga aatgaaggat ctgctttcag gtcttcatgc
     5821 tcgttttatc gagattgatg ccaaggagca tgatcgtgtc acttctcaga ttagccattt
     5881 tcctcatatt ttggcttcta gtctcatgga gcagactgcg gtctatgctc aagagcatga
     5941 gatggcaagg cgctttgcgg caggtggttt tcgagatatg acccgaattg cggaaagcga
     6001 gccaggaatg tggacctcca ttctcttgtc caatagcgag accattctgg atagaattga
     6061 ggatttcaag gaacgtttgg aagtgattgg tcaggccatt agtaagggag atgaagagca
     6121 aatttggaac ttttttaacc aagcgcgtga acaacgccag accatggaaa tccataagcg
     6181 tggtggtgtg gatagctctt acgacctcta tgttgacgtt cctgatgaag aagatgtcat
     6241 cctgcggatt ttggaactgc tacgtgggac ttccttggtt aacatccaca tcaacgagga
     6301 aaatcgtgag gatattcacg gaatcctaca aatttcattt aaaaatgctc aagacttgga
     6361 aagagctgag catctcataa cagaaaatac cgactataca gtcgtcatca aataaggaga
     6421 agaacatgtc aaatatttac gatagtgcaa acgaactcag tcgcggtcta cgcggattac
     6481 cagaatacaa ggctgttaaa gcagctaaag atgcgattgc agcagatgct gaggcaagca
     6541 aaatctttac agaatatctt gcctttcaag aggaaattca aaaactagca catacaggtc
     6601 aaatgccaga tgcgtccttc caagctaaga tggaaggctt tggtaaacag attcaaggaa
     6661 atagcctctt gtcagaattc tttaccaagc aacaacaatt ggccatttac ctttctgaca
     6721 ttgaaaaaat tgtttttgaa ccagtttcag aattgctgaa ataagaaaag aacgatccta
     6781 aagtcaggaa tttttaagga atttctggct ttttatgata aaataggtaa aagtattgca
     6841 agtatgaggt ccagtatgaa actaaaaaca aacattcgcc atttacatgg tagtatccgc
     6901 gtcccaggtg acaagtctat cagccaccgt tccattatct ttggaagttt ggctgagggt
     6961 gagaccaagg tttatgatat tctgcgaggt gaagacgttc tttcgaccat gcaggttttt
     7021 cgtgaccttg gtgttgaaat tgaggataaa gatggggtta ttaccattca aggtgtaggc
     7081 atggctggct taaaagcgcc acaaaatgcc cttaatatgg gaaattctgg cacctcgatt
     7141 cgcctgattt caggtgtcct tgctggtgca gatttcgaag tagagatgtt tggagatgat
     7201 agtctttcca aacgtcctat ggaccgtgtg acccttccac tgaaaaaaat gggcgtcagc
     7261 atttcagggc aaactgaacg agacttgcct ccccttcgct taaaagggac gaaaaaccta
     7321 agacctattc attatgagtt gccaattgcc tctgcccaag tcaagtcagc cttgatgttt
     7381 gcagccttac aagctaaggg ggagtcagtt attatcgaaa aagagtgcac ccgtaatcat
     7441 actgaagata tgttgaaaca atttggtggt catttaagtg tggacggtaa gaaaatcaca
     7501 gtccaagggc cacaaaaatt gacaggacag aaggtggtcg taccaggaga tatttccagt
     7561 gcagcctttt ggttagtcgc aggtttgatt gttccaaatt ctcgtctagt gctgcagaat
     7621 gtggggatca acgaaactcg caccggtatt attgatgtca ttcgtgccat gggtggaaaa
     7681 ttggaaataa ctgaaatcga tccagtcgct aaatcttcta ccttgactgt tgagtcttct
     7741 gacttgaaag gaacagagat tggtggcgct ttgattccac gattgattga tgaattgccc
     7801 attattgccc tgcttgcgac tcaagcccaa ggtgtaacag ttatcaagga tgctgaggag
     7861 ctcaaggtca aggaaacaga ccgtattcag gttgtggcag acgccttaaa tagtatggga
     7921 gcagatatta ctcctacggc agatgggatg attatcaaag ggaaatcagc tcttcacggt
     7981 gctagagtca atacgtttgg tgaccaccgt atcggcatga tgacagctat cgcagccctc
     8041 ttggttgcag atggagaggt ggagcttgac cgtgcagaag ccatcaatac cagctatcct
     8101 agtttctttg atgatttgga gagcttgatt catggctaag gtgttattag ggtttatggg
     8161 ggctggaaaa tcgactattg caagaggctt ggaccctaat taccttgata tggatgctct
     8221 gattgagaat cgcctaggta tgtccattgc gaattttttc gctgaaaagg gagaaacagc
     8281 ctttcgtcag gtagaatcag aagtcctagc tgatttacta caaacagacc aagtcgtgtc
     8341 aactggagga ggagtggtta tttctcagag aaatcgtgac ttactcaaga ctaatacaga
     8401 taacatctac ctgagagcag attttgaaac cctctaccaa cgtatcgcag ctgataagga
     8461 caatcagcga ccgctttttc taaataatag caaggaagaa ctagtagcta ttttccaaga
     8521 aagacaggct tggtatgagg aagtggctag tcgggttttg gatgtgacca agctaagccc
     8581 agaggaaatt atagaggaac taagatgaaa attgcttatc taggtcccaa gggatcattt
     8641 tcacaccacg ttgtgcagac agcttttcct catgaggaat tgcaggcttt tgccaacatt
     8701 acagatgtca tcaaggctta tgagcaagga ttagtgaatt attctgtggt gccagttgaa
     8761 aattctattg agggtagtgt tcatgaaacc ttggactatc tttttcatca ggctcacatc
     8821 caagcagtag cagaaatcgt tcagcctatt catcagcagt tgatggtggt tccaggccat
     8881 actaagattg aaaagatttt ttcacatcca caggccttgg ctcaaggaaa gaaattcatc
     8941 gatgaacaat atccagaggc tcaaatcgag gtaacagcta gtacagctta tgcggcccgt
     9001 tttatttccg aacatccaga ccagcccttt gcagcagttg cacctagaag ttctgctgaa
     9061 gaatatggat tggaactgat tgccgaggat attcaggaaa tggaagccaa tttcacacgt
     9121 ttctgggttc taggagctga aaagcctagt attcccttgc aagcacaaac tgaaaagatg
     9181 agtttggcct tgacattacc tgacaacctt ccaggtgcac tttataaggc cctgtcgacc
     9241 tttgcttggc gaggaattga cttgacaaaa attgaaagtc gtccactcaa gacagcactg
     9301 ggtgaatact ttttcattat cgatgtggat tataccgata aggacttggt ccactttgcc
     9361 caaaaagaat tagaagcgat tggaatccag tataaaattc tgggtgccta tcctatttat
     9421 ccaatatcag accatggaaa ggagagaaga tgagtaaaga aaatccctta agtcatcatg
     9481 agcagttgcg ttatgattat ttgctaaaaa atattcacta tctcaatgag agagaaaaaa
     9541 atgagtttgc ctatttgcaa gaaaagctaa ctcttgctag gggaaatagt agctctagct
     9601 tggaacaaga aagagaagag caggttgact taccaagcta tgcgaaccgg agtcgcttac
     9661 aatccaaatc acaagcactc tctttccctc caaaaaagaa aagacggaag ctccgtctta
     9721 aacgaatttt tatggtgatt ttttcacttt tggtctgtgt ggctttggcc atggtattca
     9781 tgtttttgcg tggttaccaa gatgctagcg caaagaaaac tgctgatgcc cgggcagctc
     9841 aagtagaagt ctttaatggt caggacacta gagatggagt taatatttta atcatgggta
     9901 ctgatggtcg aatcggccag aacagtgttg agacacgaac tgactctatt atggtattaa
     9961 atgtcggggg ctcagataag aaaatgaagc tagtcagttt catgcgtgat aatttggtct
    10021 atatagatgg ttatagtcaa gtgattaacg gtagaaaaca gacagataac aagttaaacg
    10081 tagcctacga gttaggagaa caagaggggc aaaaaggggc agaaatggtt cgccaagtct
    10141 tgaaagataa ttttgacttg gacattaagt actacgcctt ggttgatttc caggcctttg
    10201 ccacagcgat tgacacgctt ttccctgatg gggtgacgat tgatgctcaa ttttcaacat
    10261 tgaatggtcg tcccctaaca gaagctacag tcggagatga tttatacgct accgagactg
    10321 agtctccaac ccaaaccatt aaagtcggaa aacagcagat gaatggctcg accctgctca
    10381 attatgctcg tttccgtgat gatgatgagg cggattacgg ccgtacaaaa agacagcaac
    10441 aagttttaac agcaatttta gagcaaatta aagatcccac taaacttttc actggttctg
    10501 aagctcttgg aaaggttttt gctatgacct caaccaacgt accctatact ttcctcctaa
    10561 caaacggttt atctgttttg gatggagcaa aaaatggtat tgaaaaattg acgattccag
    10621 aactgggtga ctgggtagat gcctatgatg tttatggagg cttgggcttg ctggttgatc
    10681 aaaacaaata tcaaaccaag ctcgctcaaa tgggcttgag ataagataat actaaatgaa
    10741 aatcaaagtg caaactagga agctagccgc aggctgctaa aaacacagtt ttgaggttgt
    10801 agataagact gacgaagtca gttacctata cctacggcaa ggtgaagctg acgtggtttg
    10861 aagagatttt cgaagagtat aagacagaaa aatcaaagtt tgatagctat ttggatagca
    10921 gtcaggcttt gattttttac agtctgcaat agattttcac cccttgtttg tggtataatg
    10981 aaacgatacg atagaaagaa taatgaacaa tatgactgat ttaaaagcaa ttcaggctcg
    11041 tagtctggag atggctgaat attttgtggc cttttgtaaa gaacatgatt tgctctgtta
    11101 tctctgtggc ggaggcgcta ttggtgccct tcgaaacaag ggatttattc cttgggacga
    11161 cgacctagac ttttttatgc ctcgtaaaga ttatgagaaa ttagcagaat tatggcctcg
    11221 ttatgcagat gaacgttatt tcttgtcaaa gagtcacaag aattttgttg atcgtaatct
    11281 ttttattacc attcgtgaca aggaaaccac ctgtatcaag ccttatcagc aggatttgga
    11341 tttgccacat ggtctggcct tggatgtttt gcctttggat tattatccga aaaatccagc
    11401 tgagcggaaa aaacaggttc gttgggcctt gatttattca ctcttttgtg cgcaaactat
    11461 tccagaaaag catggtgctc tcatgaaatg gggaagtcgc attttactgg gtttgactcc
    11521 aaaatctctc cgttatcgca tctggaaaaa agctgagaaa gaaatgacta agtatgattt
    11581 ggctgattgt gatggcatta cagaattatg ctcaggtcct ggctacatga gaaacaagta
    11641 cccaatcaca tcttttgaat acaatctttt cttgccattt gaaggaacag agatgcctat
    11701 tccaatcggc tatgatgtct atctcagaac tgcttttggg gattatatga cgcctccacc
    11761 agcagacaag caggtaccgc atcatgatgc tgtcatcgct gatatggata agtcttatac
    11821 agaatacaag ggagaatatg gtggctaaga aaaaaatctt attttttatg tggtcttttt
    11881 ctcttggagg tggtgcagag aagattctat caaccattgt ttcaaatctg gatccagaaa
    11941 agtatgatat tgatattctt gaaatggagc actttgacaa gggatatgaa tctgttccaa
    12001 agcatgtacg cattttaaaa tcccttcaag attatcgcca aactagatgg ttacgagctt
    12061 ttttgtggag aatgagaatt tattttccaa gactggctcg tcgtttgctt gtaaaagatg
    12121 attatgatgt tgaagtttct tttaccatta tgaatccacc actgttgttc tctaaaagaa
    12181 gagaagtcaa gaagatatct tggattcatg gaagtattga agaatttctt aaggatagct
    12241 ctaaaagaga atcacataga agccagttgg atgctgcgaa tacaattgta gggatttcaa
    12301 aaaaaaccag caattctatc aaggaagttt atccagatta tgcttctaaa ttacagacaa
    12361 tctacaatgg atatgatttt cagactattc tagaaaaatc tcaagagaag atcgatatcg
    12421 agattgctcc tcaaagtatc tgtactatcg gacggattga ggaaaataag ggttctgacc
    12481 gtgtagtgga agtgatacga ttattacacc aagagggaaa aaactatcat ctctatttta
    12541 tcggggctgg tgatatggaa gaggaactga aaaaacgagt caaagagtat gggattgagg
    12601 actatgtaca tttccttggt tatcaaaaaa atccttatca gtatttatct cagacgaaag
    12661 ttctcttgtc tatgtctaaa caagaaggtt ttcctggagt gtatgtggag gccttgagtc
    12721 tgggactccc ttttatctct acggacgttg gaggggctga ggaattatcc caagaaggac
    12781 gatttggaca aatcattgag agcaatcaag aggcagctca ggcgattact aattacatga
    12841 cttctgcctc aaactttgat gtcgatgagg ctagccaatt cattcaacaa tttacaatta
    12901 caaaacaaat cgaacaagta gaaaaactat tagaggagta gcatggaaac tgcattaatt
    12961 agtgtgattg tgccagtcta taatgtggcg cagtacctag aaaaatcgat agcttccatt
    13021 cagaagcaga cctatcaaaa tctggaaatt attcttgttg atgatggtgc aacagatgaa
    13081 agtggtcgct tgtgtgattc aatcgctgaa caagatgaca gggtgtcagt gcttcataaa
    13141 aagaacgaag gattgtcgca agcacgaaat gatgggatga agcaggctca cggggattat
    13201 ctgattttta ttgactcaga tgattatatc catccagaaa tgattcagag cttatatgag
    13261 caattagttc aagaagatgc ggatgtttcg agctgtggtg tcatgaatgt ctatgctaat
    13321 gatgaaagcc cacagtcagc caatcaggat gactattttg tctgtgattc tcaaacattt
    13381 ctaaaggaat acctcatagg tgaaaaaata cctgggacga tttgcaataa gctaatcaag
    13441 agacagattg caactgccct atcctttcct aaggggttga tttacgaaga tgcctattac
    13501 cattttgatt taatcaagtt ggccaagaag tatgtggtta atactaaacc ctattattac
    13561 tatttccata gaggggatag tattacgacc aaaccctatg cagagaagga tttagcctat
    13621 attgatatct accaaaagtt ttataatgaa gttgtgaaaa actatcctga cttgaaagag
    13681 gtcgcttttt tcagattggc ctatgcctac ttctttattc tggataagat gttgctagat
    13741 gatcagtata aacagtttaa agcctattct cagattcatc gttttttaaa aggccatgcc
    13801 tttgctattt ctaggaatcc aattttccgt aaggggagaa gaattagtgc tttggcccta
    13861 ttcataaata tttccttata tcgattctta ttactgaaaa atattgaaaa atctaaaaaa
    13921 ttacattaga acgggggtga ccaagtgatt gatgggaaac gattattatt tagtttgacc
    13981 atagtcagtt atgccttgac gctagtaagt ggaattgtgt atctgtttaa taataataat
    14041 gttagcttac tttctacttt attgttcttg ttggttagta gcttaattgc ttgttggaat
    14101 gatatcaagt attacttaat ccattttatt ttctatttaa ccatttttgt atttctggta
    14161 tcaagaccga ccattgatta ttttagggat ggtgctttgg atacctatca tccaatagcc
    14221 tatcgttttg cctttatagt tgtcatggtt tcgattctgg gcttgaccac aggaggcatt
    14281 ctggctcgtt acttcatagc taggaagaaa ataaaagtag caaatatagg aaattctcta
    14341 aaagaggttt atatcaagcg gttacgcttt gtatcactag gagtttttct tctaacctat
    14401 cctttctatt tcattcggtt atttgaacgg ctcttgtatc gtttgcagac ttcctactat
    14461 gcctactatg caaattttga aagtaaactg ccttatttta cctacatttt gtctaccttt
    14521 acggtctatg caatgtgtat gtatctggca accaagccaa agaaattgca ggccacagca
    14581 gtgcttgtct cctttattgc agctaatact attcatttgg caattgggac acgaaatccc
    14641 tttattttaa gtattttatt tgcttttgtt tattacttta tgcgggagca aactgaaaaa
    14701 ggaaaatgga ttgggtttaa agaaaagtta gcgatttttg taggttctcc tattctcatg
    14761 ttagcgatgg gagtactcaa ttatgtacgg gataatgtcc aagtttccca tacaggtttc
    14821 tgggatatct tacttgactt tatctataaa caagggacta gttttggtgt tctggctcga
    14881 ggttttctat ttaacagtag cctcccttac cgagatttcc gtaattttac ttttggtcct
    14941 gttcttgatt attttgcaag ggggagtttg ggagccattt tcggaggaaa agcctttgaa
    15001 catacaacca atagtgtgga actagctatt gatagtaata gttatgccca caatctatcc
    15061 tatcttgtct tgaacaagga atacttgaaa gggcatggta tcggaagtag ttatatcatg
    15121 gagttgtata ccgactatgg tatgattgga gtctttctgc ttagtttctt actcggcgta
    15181 ttatttatag ccatgctgca agtagcctat cgctcaagga caatcctatt tgctttatcc
    15241 ctactcatct tgaataatct attctttatg ccaagaagca gcttttcaga aagtttcttc
    15301 aatttattta caatgcaatt ctggggaatt gttcttgtga ttatatttgt agcaaaaatg
    15361 cttacaaagg aaaactagta tctactaaac aaaggagaaa aaaatcatgt ttgaacatta
    15421 ttcagtagct gatttgtttg caaatcttta taaaaaacga aaagcaaata ttttagctct
    15481 catcgcttta tttgctctca ttgctgtacc atttacaatt aaagcagtta ggaataaaaa
    15541 cactgtcaaa gatacaacaa gttattcaac ttatcttatc tataaaatca ctcctccaga
    15601 agattcggcc aaaacgattt tgaatcatca aattggtggt tatagtgatt tttatgggaa
    15661 attgattgat ggtaatttga atggagctta tcttttcaat gatgtagaac ccagtgagtt
    15721 gaaaaaaatt gccagtgaat tagacacgac ggaaacaacc ttgaaaaatt caacgaatga
    15781 ctattggtgg aaaaaattga ccgtctacta tatgattgac gatgcagggg ttggtgtgaa
    15841 aattttgaca tcaagtaaag atgccaatga cttgttagag aaaaaaattg atgggttgat
    15901 tgagaaattt aaacatgctt atgcaaatgt gaaaattgaa aaactggaaa ccatcaactc
    15961 taaagaattg aacgcaaatg gtgaaacagc gcttggctta aatgtgaaaa atctgattct
    16021 tcgtttagtt gttattggag tggtttgtgt gattttggtt gtgatgggaa atgtgttagt
    16081 ttatctcttt aatccaacaa tcaatagagt aggtgatttt tctcagtatc aaattgattt
    16141 tgtaacagag attacaacaa ttgctaacct agcagatgtt ttgtcataca aaaatactgg
    16201 acaggaattg accatcgtta gctcaaataa agctatccta gataaattga aacagagtca
    16261 agaagcttta aaaggaatgc attttgtaga tttgcaggat gtatcatctc ttttggaaag
    16321 agatacagtc cttcttgttg aagagtacgg agtgactcgt tataagaaat ttgagcaaag
    16381 tcttcaaatt ctcagaaact taaatcgttc tatcctcggt gtagcaacct ttaaattata
    16441 agtattctga tgtataaggt cttcaaaatc ggctcttagc cgattttgaa ggctttttct
    16501 atctgtctgc agaaattttc tcttggagtc tcctatagaa agaccctagg gaaaatctat
    16561 ccgtatagag gagccttctt ttgataaaat cgtaaaaatc tagtataata gatagaactg
    16621 aaagtatgag gttactagca atgaaaatga aacaaattag tgatacaact ttaaaaatca
    16681 cgatgtcttt agaggatttg atggatcgtg gaatggagat tgctgacttt ctcgttcctc
    16741 aagaaaaaac agaagagttc ttttatgcta tcttggaaga gctagagatg cctgatagct
    16801 ttctggatac aggcatgttg agcttccgtg tgactccaaa acctgataag gtagatgtct
    16861 ttgtgaccaa gtcaaagatt gaccaaaatc tagattttga agacttatcg gatttgccag
    16921 atatggaaga attggctcaa atgtctccag atgaatttat caaaaccctg gaaaaaagca
    16981 tcgcagacaa aaccaaggat gatatcgaag ccattcaatc tcttgagcaa gttgaagcca
    17041 aggaagaaga gcaagagcag gctgaacaag aagctgagag taagaaagaa ccttacatct
    17101 actacatcct ttcttttgct aagttggctg acttggtagc ttttgccaag acagtgactt
    17161 ttgaaatgga aacttctgaa ctctacaaaa tgaacgagcg ctattatttg accattttag
    17221 tggatattga aaatcatcca agcccatatc cagcttggct gttggcccgt atgcgcgagt
    17281 ttgcagacga tagtgatatc agtcgctcag tcttacaaga gtatggtcaa gtcttgatga
    17341 gtcacgatgc agtgctcaat ctgcaaaaaa tcggctaatc ctttctggaa agctattttc
    17401 agattttaag aagagcgaat cgtctgattc gttttttctt tttatactga aatagtgatt
    17461 tactataata ggaattttca caaaattctg ttataatggc tatattagaa aatttcgagg
    17521 agataaagat gacagttaaa attgctttac taggatttgg taccgttgca agtggtgtgc
    17581 ctttcctcct aaaggaaaat ggaggaaaaa tcaatcaatc agcacattca gatatcaaag
    17641 ttgctaaggt attggtcaag gatgaagatg aaaaaaatcg cttgcttgca gcagggaatg
    17701 actttaactt tgtaaccaat gtggatgata ttttatcaga ccaggatatt actatcgtag
    17761 tggaattgat ggggcgtatt gagcctgcta aaacctttat cactcgtgcc ttggaagctg
    17821 gaaaacacgt tgttactgct aacaaggacc ttttagctgt ccatggcgca gaattgctag
    17881 aaatcgctca agctaacaag gtagcacttt actacgaagc agcagttgct ggtgggattc
    17941 caattcttcg tactttagca aattccttgg cttctgataa aattacgcgc gtgcttggag
    18001 tagtcaacgg aacttccaac ttcatggtga ccaagatggt ggaagaaggc tggtcttacg
    18061 atgatgctct tgcggaagca caacgtctag gatttgcaga aagcgatccg acgaatgacg
    18121 tagatgggat tgatgcagcc tacaagatgg ttattttgag ccaatttgcc tttggcatga
    18181 agattgcctt tgatgatgta gcccacaagg gaatccgcaa tatcacacca gaagacgtag
    18241 ctgtagctca agagcttggt tacgtagtga aattggttgg ttctattgag gaaacttctt
    18301 caggtattgc tgcagaagtg actccaacct tcctacctaa agcgcaccca cttgctagtg
    18361 tgaatggcgt aatgaacgct gtctttgtag aatctatcgg tattggtgag tctatgtact
    18421 acggaccagg tgcgggtcaa aaaccaactg caacaagtgt tgtagctgat attgtccgta
    18481 tcgttcgtcg tttgaatgat ggtaccattg gcaaagactt caacgaatat agccgtgact
    18541 tggtcttggc aaatcctgaa gatgtcaaag caaactacta tttctcaatc ttggctccag
    18601 actcaaaagg tcaggtcttg aagttggctg aaatcttcaa tgctcaagat atttccttta
    18661 agcaaatcct tcaagatggc aaagagggtg acaaggcgcg tgtcgttatc atcacacaca
    18721 agattaataa agcccagctt gaaaatgtct cagctgaatt gaagaaggtt tcagaattcg
    18781 acctcttgaa taccttcaag gtgctaggag aataagatga agattattgt acctgcaacc
    18841 agtgccaata tcgggccagg ttttgactcg gtcggtgtag ctgtaaccaa gtatcttcaa
    18901 attgaggtct gcgaagaacg agatgagtgg ctgattgaac accagattgg caaatggatt
    18961 ccacatgatg agcgtaatct cttgctcaaa atcgctttgc aaattgtacc agacttgcaa
    19021 ccaagacgct tgaaaatgac cagtgatgtc cctttggcgc gcggtttggg ttcttccagc
    19081 tcggttattg ttgctgggat tgaactagcc aaccaactgg gtcaactcaa cttatcagac
    19141 catgaaaaat tgcagttagc gaccaagatt gaagggcatc ctgacaatgt ggctccagcc
    19201 atttatggta atctcgttat tgcaagttct gttgaagggc aagtctctgc tatcgtagca
    19261 gactttccag agtgtgattt tctagcttac attccaaact atgaattacg tactcgcgac
    19321 agccgtagtg tcttgcctaa aaaattgtct tataaggaag ctgttgctgc aagttctatc
    19381 gccaatgtag cggttgctgc cttgttggca ggagacatgg tgaccgctgg gcaagcaatc
    19441 gagggagacc tcttccatga gcgctatcgt caggacttgg taagagaatt tgcgatgatt
    19501 aagcaagtga ccaaagaaaa tggggcctat gcaacctacc tttctggtgc tgggccgaca
    19561 gttatggttc tggcttctca tgacaagatg ccaacaatta aggcagaatt ggaaaagcaa
    19621 cctttcaaag gaaaactgca tgacttgaga gttgataccc aaggtgtccg tgtagaagca
    19681 aaataaagaa tagaagatag gatggggaaa ctcttgacca gagggtttca tatccttttt
    19741 gtgaaaagaa gtttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggt
    19801 tgctcaaagc actgctttga ggttgtagat aagactgacg aagtcagctc aaaacagtgt
    19861 tttgaggttg cagatagaac tgacgaagtc agctcaagac actgttttga ggttgcagat
    19921 agaactgacg aagtcagtaa catatatacg gtaaggtgac gctgacgtgg tttgaagaga
    19981 ttttcgaaga gtattagtta aaaacgtgat aaaggagaaa taaaaatgac agaaatttat
    20041 ctagcaggtg gttgtttttg gggcctagag gaatattttt cacgcatttc tggagtgcta
    20101 gaaaccagtg ttggctacgc taatggtcaa gtcgaaacga ccaattacca gttgctcaag
    20161 gaaacagacc atgcagaaac ggtccaagtg atttacgatg agaaggaagt gtcactcaga
    20221 gagattttac tttattattt ccgagttatc gatcctctat ctatcaatca acaagggaat
    20281 gaccgtggtc gccaatatcg aactgggatt tattatcagg atgaagcaga tttgccagct
    20341 atctacacag tggtgcagga gcaggaacgc atgctgggtc gaaagattgc agtagaagtg
    20401 gagcaattac gccactacat tctggctgaa gactaccacc aagactatct caggaagaat
    20461 ccttcaggtt actgtcatat cgatgtgacc gatgctgata agccattgat tgatgcagca
    20521 aactatgaaa agcctagtca agaggtgttg aaggccagtc tatctgaaga gtcttatcgt
    20581 gtcacacaag aagctgctac agaggctcca tttaccaatg cctatgacca aacctttgaa
    20641 gaggggattt atgtagatat tacgacaggt gagccactct tttttgccaa ggataagttt
    20701 gcttcaggtt gtggttggcc aagttttagc cgtccgattt ccaaagagtt gattcattat
    20761 tacaaggatc tgagccatgg aatggagcga attgaagttc gttctcgttc aggcagtgct
    20821 cacttgggtc atgttttcac agatggaccg cgggagttag gcggcctccg ttactgtatc
    20881 aattctgctt ctttacgctt tgtggccaag gatgagatgg aaaaagcagg atatggctat
    20941 ctattgcctt acttaaacaa ataaaacaga gagtggggct tcccactttc ttcatttcta
    21001 gaatatgaat agaagggatt tatgaaacac ctattatctt acttcaaacc ctacatcaag
    21061 gaatcaattt tagccccctt gttcaagctg ttagaagctg tttttgagct cttggttccc
    21121 atggtgattg ctgggattgt tgaccaatct ttacctcagg gagatcaagg tcatctctgg
    21181 atgcagattg gtctgctcct tatctttgca gtaattggcg ttttagtggc cttgatagct
    21241 caattttact cagcaaaggc agcagtaggt tttgctaagg aattgacaaa cgatctttat
    21301 cgtcatattc tttccttgcc caaggacagc agagaccgtc tgacaacttc tagtttggtc
    21361 actcgcttga cttcggatac ctaccagatt cagactggta tcaatcaatt cctgcgtctc
    21421 tttttacgag cgcccattat cgtttttggt gccattttta tggcttatcg aatctcagct
    21481 gagttgactt tctggttctt agtcatggtt gccattttga ccattgtcat tgtagggtta
    21541 tctcgattgg tcaatcctct ctacagtagt ctcagaaaga aaacggacca actggttcag
    21601 gaaacgcgcc agcaattgca agggatgcgg gttattcgtg cttttggtca agaaaaacga
    21661 gagttacaga tttttcaaac ccttaaccaa gtttatgcta aattacaaga aaagacaggt
    21721 ttcttgtcta gtttattaac acctctgacc tatctgattg tcaatggaac tcttctcgtt
    21781 attatctggc aaggctatat ttcaattcaa ggaggagtgc tcagtcaagg tgctctcatt
    21841 gctcttatca attacctctt acagattttg gtggaattgg tcaagctagc catgttgata
    21901 aattctctca accagtccta tatctcagcc aagcgaatcg aggaagtctt tgttgaggct
    21961 ccagaggata tccattcaga gttagaacaa aagcaagcta ccagtgatcg ggttttacaa
    22021 gtccaagaat tgacctttac ctatcctgat gcagcccagc cttctctgag agacatttcc
    22081 tttgatatga ctcaaggaca aatccttggt atcattgggg ggactggttc tggtaaatca
    22141 agcttggtgc aactcttact tggactttat ccagtagaca aggggaacat tgacctttat
    22201 caaaatggac gtagtcctct taatttggag cagtggcggt cttggattgc ctatgtacct
    22261 caaaaggtcg aactctttaa gggaactatt cgttccaact tgactctagg tttaaatcaa
    22321 gaagtgactg atcaagaact ctggcaagcc ttggaaattg ctcaagctaa ggattttgtc
    22381 agtgataagg aaggactctt ggatgctcta gttgaagcag gagggcgaaa tttctcaggt
    22441 ggacaaaaac aaaggctgtc tatcgcacga gcagtcttgc gccaggctcc gtttctcatc
    22501 ctagatgatg cgacctcagc actggacacc attacagagt ccaagctctt gaaagctatt
    22561 agagaaaatt ttccaaacat gagcttaatt ttgatctctc aacgaacctc gactttacag
    22621 atggcggacc agattctcct cttggaaaaa ggtgagttgc tagctgttgg caagcacgat
    22681 gacttgatga aatccagcca agtctatcgt gaaatcaatg catcccaaca tggaaaggag
    22741 gactagaatg aaacgacaaa ctgtaaacca gacgctcaaa cgtttagccg tagatttagc
    22801 aaaccatccc ttcctccttt tcctagcctt tctaggaact attgcccaag ttggcttatc
    22861 aatttaccta cctattctga ttgggcgggt cattgaccaa gtcctagtgg ctggttcatc
    22921 accagttttt tggcagattt ttctccagat gctcttggtg gtaataggaa atactctagt
    22981 acaatgggcc aatcctctcc tctataatcg tctaatcttc tcttatacca gagatttacg
    23041 ggagcgaatc atccataaac tccatcggtt accgattgcc tttgtagata ggcaaggtag
    23101 tggagagatg gttagtcgtg taaccacaga catcgaacag ttggcagctg gcttgaccat
    23161 gatttttaac caatttttca ttggtgtttt gatgattttg gttagtattc tagccatgct
    23221 ccaaattcat ctcctcatga ctctcttagt cttgctgttg acgccactgt ccatggtgat
    23281 ttcacgcttt attgccaaga gatcctatca tctcttccag aagcaaacag agacgagggg
    23341 gattcagacc cagttgattg aagaatcgct tagccagcag actataatcc agtccttcaa
    23401 tgctcaaaca gagtttatcc aaagattgcg tgaggctcat gacaactact caggctattc
    23461 tcagtcagcc atcttttatt cttcaacggt caatccttcg actcgctttg taaatgcact
    23521 catttatgcc cttttagctg gagtaggagc ttatcgtatc atgatgggtt caaccttgac
    23581 cgtcggtcgt ttagtgactt ttttgaacta tgttcagcaa tacaccaagc cctttaacga
    23641 tatttcttca gtgctagctg agttgcaaag tgctctggct tgcgtagagc gtatctatgg
    23701 agtcttagat agccctgaag tggctgaaac aggtaaggaa gtcttgacca gtgaccaagt
    23761 taagggagct atttccttta aacatgtctc ttttggctac catcctgaaa aaattttgat
    23821 taaggacttg tctatcgata ttccagctgg tagtaaggta gccatcgttg gtccgacagg
    23881 tgctggaaaa tcaactctta tcaatctcct tatgcgtttt tatcccatta gctcgggaga
    23941 tatcttgctg gatgggcaat ccatttatga ttatacacga gtatcattga gacagcagtt
    24001 tggtatggtg cttcaagaaa cctggctcac acaagggacc attcatgata atattgcctt
    24061 tggcaatcct gaagccattc gagagcaagt aattgctgct gccaaagcag ctaatgcaga
    24121 ctttttcatc caacagtttc cacagggata cgataccaag ttggaaaatg ctggagaatc
    24181 tctctctgtc ggccaagctc agctcttgac catagcccga gtctttctgg ctattccaaa
    24241 gattcttatc ttagacgagg caacttcttc cattgataca cggacagaag tgctggtaca
    24301 ggatgccttt gcaaaactca tgaagggccg cacaagcttc atcattgctc accgtttgtc
    24361 aaccattcag gatgcggatt taattcttgt cttagtagat ggtgatattg ttgaatatgg
    24421 taaccatcaa gaactcatgg atagaaaggg taagtattac caaatgcaaa aagctgcggc
    24481 ttttagttct gaataagcca ttctcttttg aaagtttctg gacgaaaaaa gttgccttcg
    24541 agtgactttt ttgttacaat agctagaaaa attgttcact gtaatactca atgaaaatca
    24601 aagagcaaac taggaagcta gccgtaggtt gctcaaagca cagctttgag gttgtagata
    24661 agactgacga agtcagctca aagcactgtt ttgaggttgc agatagaact gacgaagtca
    24721 gctcaaaaca ctgttttgag gttgcagata gaactgacga agtcagctca aaacactgtt
    24781 ttgaggttgc agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga
    24841 agtggtttga agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc
    24901 agcatgtaaa tatagtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag
    24961 cagtcaagga ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag
    25021 ctgagcagat tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca
    25081 cccattctgc aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc
    25141 tcaatgacta tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg
    25201 tcaaagaagc cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata
    25261 atcccaatgg tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt
    25321 gttatttttc tccgactctc ttttcttcag agacagaaac aactgctgag actataagca
    25381 gaactcgatc catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg
    25441 tagcacctca ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg
    25501 caaaagagct aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa
    25561 ttatcctcaa acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag
    25621 atcatccgtc cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg
    25681 catcttctca agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa
    25741 ttgctccaat tatccagctc caaaaagcgg gagtagcagt cggaattgcg actgactcgg
    25801 ttgcttccaa taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga
    25861 agatgaaaag tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa
    25921 tcgaaggggc taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc
    25981 aagcagattt tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata
    26041 tgctgtctca cctggtttat gcagttaaat ctaatgatgt agatgatgtt tatatcgccg
    26101 gagaacaggt tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg
    26161 aaaaatttta aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta
    26221 tatacggttt aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc
    26281 gaggacaaga cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg
    26341 cgtttttctt tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga
    26401 gtgaagcaat tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag
    26461 ctgctgcatc tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc
    26521 ttcgtcgtga gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc
    26581 gtcgtgcagc tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg
    26641 cagtagcatt ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta
    26701 aaaacgctga agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag
    26761 aagagattct tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt
    26821 ctgtacttca agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca
    26881 aagaagacgc ggcttaatct taagctacac agcgtagtct agctacgaaa aaaactatta
    26941 taaaatttaa aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg
    27001 ctgaaattaa agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag
    27061 aatttggtgt aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg
    27121 gtgctgctaa agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg
    27181 ttatcaaagt tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg
    27241 acggtgcacc agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaag
    27301 ctaaattgga agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat
    27361 tgcggaatcc ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat
    27421 tgatttggtt tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg
    27481 gtagacacta gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag
    27541 gacagtcatc acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg
    27601 taaaagcaac tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat
    27661 ttggaaatgt tggaatttta gtcttggagg gacaataatg aagcgtatta ccgctaatca
    27721 atatcagaca tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta
    27781 taagaatatg aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt
    27841 gtctttgagc aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc
    27901 aaatttgatg gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag
    27961 taacattttt agatgattac cataaaaaac acaactaccc actattttac gagtcctatc
    28021 ttcaaaacgt tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg
    28081 tagatgatca tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa
    28141 aagagggaat acttacaacc caagtaactg taaaagctta tgatgaagac aagaaaccga
    28201 ttaacttcgc aaatttatta gattccttaa tcgtctcgga atatcaaatg gaaccgaatc
    28261 tttgggaggt ctcccatgat tgatctctat ctaagtaaaa atagacaaag aaatcaactt
    28321 cttttagatt tcttccaaaa ctatggcata gaggtatctt gtcattcagt ttctgaaatg
    28381 acaaaggaca aattaattga gatgatgagc tattcttcag attgttttga gtttttatct
    28441 cccaatttat tacgatttaa gaaccgtgac aatctaagat taacggattt cgttgagata
    28501 attttaaaaa atcctgaact aaccatcaga cttcctcttg cggtttcaaa taagcgagtt
    28561 tatccaagtc tgaatttgga agaggctaga gctctattgc caagagatac gaaacaattg
    28621 atttacatgg cgcaaacaca ttatttatct aactaaagga gaagcaatat ggctgaacga
    28681 ttttgggaga acttgtccat tattttggct gaaagaaata tcagctgtat tgagttaacc
    28741 agaaaaatgt ttgcgggaga gtttcactat ccaagtgaat tgaatcgttt gtatcaaaag
    28801 attcgccact agaagatgga acaacgaatg cctcaaagtt catgggtaga aaggattgtg
    28861 caggtattag atttagatta tgaagattta tttcggaggt aactatgaaa agaataattc
    28921 cagtttatat attccaacaa gtaaatgtcc tattggtatc tctatactta ttgaaatttc
    28981 tttgtatcgg tgagttaact atactacaga ttctctatgg ttcgtcactc atttcttttt
    29041 tatggatgta tggccaacgc aaacaagctc ataaggtaaa tatgaaatct aggatgaaat
    29101 ggcttggtat tggattcgtt agcctactga ttataagtct atgttttagt ctaatccatg
    29161 cgcaagaaag cacgaatcaa gcaaacttaa ttgggcttca acatcaagtt ccttggtttt
    29221 catttttatt gtttttaatc aatgcgagta tggttgaaga atttctgtat agagaaattt
    29281 tatggaactt ggttagaaaa ttagatattc gagttgcttt gacaagtgtt ttatttgcct
    29341 tagcacatca tccaggaacc attatagctt ggtgtttgta tgtatcgctg gggatgtttt
    29401 tagggcttgt acgctacaaa ttggacttat ggggcagtat ggggctacat ttagtgtgga
//
LOCUS       DAVLSL010000009        28987 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLSL010000009 DAVLSL010000000
VERSION     DAVLSL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.009"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..871
                     /gene="nanB"
                     /locus_tag="VXN30_000307"
     CDS             <1..871
                     /gene="nanB"
                     /locus_tag="VXN30_000307"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1356982.1"
                     /translation="EVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSLFK
                     VTPTNYIAMTTSQNRVESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFATYT
                     SGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYMTS
                     RDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQLVV
                     GLVNKEDDSIDWRYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLSNV
                     VQYIDLEINDLTK"
     gene            883..1986
                     /locus_tag="VXN30_000308"
     CDS             883..1986
                     /locus_tag="VXN30_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1356983.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            2160..2858
                     /locus_tag="VXN30_000309"
     CDS             2160..2858
                     /locus_tag="VXN30_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1356984.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            2877..4394
                     /locus_tag="VXN30_000310"
     CDS             2877..4394
                     /locus_tag="VXN30_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1356985.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            4519..5847
                     /locus_tag="VXN30_000311"
     CDS             4519..5847
                     /locus_tag="VXN30_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1356986.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            6099..6965
                     /locus_tag="VXN30_000312"
     CDS             6099..6965
                     /locus_tag="VXN30_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1356987.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            6981..7820
                     /locus_tag="VXN30_000313"
     CDS             6981..7820
                     /locus_tag="VXN30_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1356988.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            8093..8545
                     /locus_tag="VXN30_000314"
     CDS             8093..8545
                     /locus_tag="VXN30_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1356989.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            8642..8986
                     /locus_tag="VXN30_000315"
     CDS             8642..8986
                     /locus_tag="VXN30_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1356990.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            9270..9914
                     /locus_tag="VXN30_000316"
     CDS             9270..9914
                     /locus_tag="VXN30_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1356991.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            10083..11000
                     /locus_tag="VXN30_000317"
     CDS             10083..11000
                     /locus_tag="VXN30_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1356992.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLDTARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            11018..11902
                     /locus_tag="VXN30_000318"
     CDS             11018..11902
                     /locus_tag="VXN30_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1356993.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(11918..12769)
                     /locus_tag="VXN30_000319"
     CDS             complement(11918..12769)
                     /locus_tag="VXN30_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1356994.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            12969..15026
                     /gene="pbp2b"
                     /locus_tag="VXN30_000320"
     CDS             12969..15026
                     /gene="pbp2b"
                     /locus_tag="VXN30_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1356995.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDRSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            15037..15633
                     /gene="recR"
                     /locus_tag="VXN30_000321"
     CDS             15037..15633
                     /gene="recR"
                     /locus_tag="VXN30_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1356996.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEISEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            15809..16852
                     /locus_tag="VXN30_000322"
     CDS             15809..16852
                     /locus_tag="VXN30_000322"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1356997.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALELAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            16936..18309
                     /locus_tag="VXN30_000323"
     CDS             16936..18309
                     /locus_tag="VXN30_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1356998.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGVELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            18332..18907
                     /locus_tag="VXN30_000324"
     CDS             18332..18907
                     /locus_tag="VXN30_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1356999.1"
                     /translation="MIALTDTGLTFTKEPFDRERYEDLRSLLSEMLNQVSDLDADEVA
                     ELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEVGYSPTENILKEI
                     EEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQENQEIADLQFFAID
                     QLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            18998..19702
                     /locus_tag="VXN30_000325"
     CDS             18998..19702
                     /locus_tag="VXN30_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1357000.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            19921..21294
                     /gene="ftsA"
                     /locus_tag="VXN30_000326"
     CDS             19921..21294
                     /gene="ftsA"
                     /locus_tag="VXN30_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1357001.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            21311..22570
                     /gene="ftsZ"
                     /locus_tag="VXN30_000327"
     CDS             21311..22570
                     /gene="ftsZ"
                     /locus_tag="VXN30_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1357002.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            22575..23246
                     /locus_tag="VXN30_000328"
     CDS             22575..23246
                     /locus_tag="VXN30_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1357003.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            23256..23795
                     /locus_tag="VXN30_000329"
     CDS             23256..23795
                     /locus_tag="VXN30_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1357004.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            23795..24058
                     /locus_tag="VXN30_000330"
     CDS             23795..24058
                     /locus_tag="VXN30_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1357005.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            24055..24840
                     /locus_tag="VXN30_000331"
     CDS             24055..24840
                     /locus_tag="VXN30_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1357006.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            24849..25637
                     /gene="divIVA"
                     /locus_tag="VXN30_000332"
     CDS             24849..25637
                     /gene="divIVA"
                     /locus_tag="VXN30_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1357007.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            25985..28777
                     /locus_tag="VXN30_000333"
     CDS             25985..28777
                     /locus_tag="VXN30_000333"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1357008.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT         8764 a         5241 c         6426 g         8556 t
ORIGIN      
        1 tgaagttttg gagggaggaa agtctttaac agtcgaacaa tattcggttg attttgatag
       61 tggctcttta agagaaaggc ataatggaaa acaggttcct atgaatgttt tctacaaaga
      121 ttcgttattt aaagtgactc ctactaatta tatagcaatg acaactagtc agaatagagt
      181 agagagttgg gaacaattta agttgttgcc tccgttctta ggagaaaaac ataatggaac
      241 ttacttgtgt cctggacaag gtttagcatt aaaatcaagt aacagattga tttttgcaac
      301 atatactagt ggagaactaa cctatctcat ttctgatgat agtggtcaaa catggaagaa
      361 atcctcagct tcaattccgt ttaaaaatgc aacagcagaa gcacaaatgg ttgaactgag
      421 agatggtgtg attagaacat tctttagaac cactacaggt aagatagctt atatgactag
      481 tagagattct ggagaaacat ggtcggaagt ttcgtatatt gatggaattc aacaaacttc
      541 atatggcaca caagtatctg caattaaata ctctcaatta attgatggaa aagaagcagt
      601 cattttgagt acaccaaatt ctagaagtgg ccgtaaggga ggccaattag ttgtcggttt
      661 ggtcaataaa gaagatgata gtattgattg gagataccac tatgatattg atttgccttc
      721 gtatggttat gcctattctg cgattacaga attgccaaat catcacatag gtgtactgtt
      781 tgaaaaatat gattcgtggt cgagaaatga attgcattta agcaatgtag ttcagtatat
      841 agatttggaa attaatgatt taacaaaata aaggagaaaa acatggttaa atacggtgtt
      901 gttggagcag ggtattttgg agctgaattg gctcgctata tgcaaaagaa tgatggagca
      961 gagattactc ttctctatga tccagataat gcagaggcga ttgcagaaga attgggagca
     1021 aaagtagcaa gttccttaga tgagttggtt tctagcgatg aagtagattg tgttatcgtc
     1081 gcaactccaa ataatcttca taaggaaccg gttattaagg ctgcacagca tggtaaaaat
     1141 gttttctgtg aaaaaccaat tgcgctttct tatcaagatt gtcgcgagat ggtagatgcg
     1201 tgtaaagaaa acaatgtaac ctttatggca ggacatatta tgaatttctt taatggtgtt
     1261 catcatgcaa aagaactcat taatcaagga gttatcggag acgttctata ttgtcataca
     1321 gctcgtaatg gttgggaaga acaacaaccg tcagtatcat ggaaaaaaat tcgtgaaaaa
     1381 tcaggtggtc acttgtatca ccacatccat gaattggatt gcgttcaatt ccttatgggg
     1441 ggcatgcctg aaactgtaac catgacaggt ggaaatgtgg cccatgaagg tgaacatttc
     1501 ggtgatgaag atgatatgat ttttgtcaat atggaatttt ctaataagcg ttttgccttg
     1561 ttagaatggg gttcagctta tcgttggggt gaacattatg tcttaatcca aggaagcaaa
     1621 ggtgccatcc gcttagactt attcaactgt aaaggaactc ttaagctaga tgggcaagaa
     1681 agctatttct tgattcacga atcgcaagaa gaagatgatg atcggactcg tatctatcat
     1741 agtacagaga tggatggagc aattgcttat ggtaaaccag gtaaacgtac tccattatgg
     1801 ctatcatctg tcattgataa agaaatgcgc tatctgcatg agattatgga aggagctcca
     1861 gtatcagaag aatttgcaaa acttttgaca ggtgaagctg ccctagaagc aattgctact
     1921 gcagatgctt gtacccagtc tatgtttgaa gatcgcaaag taaaattgtc agaaattgta
     1981 aaataaattt tggtattctc ctatttatag gtcgacttgc tcctctgaaa gtacttttag
     2041 aggagctgtt tgactttgct agtttttgaa actgaaatct attatactac aaactattga
     2101 aagcgtttta attttaaggt ataataatct catagaaata aagaaaagga ggaaagagga
     2161 tgccacagat tagcaaagaa gccttgattg agcaaatcaa agatggaatc atcgtttctt
     2221 gtcaggctct tcctcatgaa ccgctttata cagaagcggg aggggtgatt cccttgctgg
     2281 tcaaagcggc tgagcaaggt ggagcagtcg gtatccgagc aaacagtgtt cgcgatatca
     2341 aggaaattaa ggaggtcact aaacttccaa tcattgggat tatcaaacgt gattatccac
     2401 ctcaggaacc cttcatcacg gctactatga aagaagttga tgaattggca gaactggaca
     2461 tcgaggtgat tgctctggat tgtaccaagc gtgaacgcta cgatggtttg gaaattcaag
     2521 agttcattcg tcaggttaag gagaaatatc ctaatcagct tttgatggct gatactagta
     2581 tcttcgaaga agggctagca gctgtagaag caggaattga ctttgtcgga acaaccttat
     2641 caggctacac atcctacagt ccaaaagtag acggtccaga ttttgaattg attaagaaac
     2701 tctgtgatgc cggtgtagat gtcattgcag aaggaaaaat tcatacacca gaacaagcca
     2761 aacaaatcct tgaatatgga gtgcgaggca tcgttgttgg tggcgccatt actagaccaa
     2821 aagagattac agaacgcttc gttgctagtc ttaaataaga tgtgaggggg agttttatgt
     2881 ttaaagtttt acaaaaagtt ggaaaagctt ttatgttacc tatagctata cttcctgcag
     2941 caggtctact tttggggatt ggtggtgcac tttcaaaccc aaccacgata gcaacttatc
     3001 caatactaga caatagtatt tttcaatcaa tattccaagt aatgagctct gcaggagagg
     3061 ttgtattcag taatttgtca ctacttctct gtgtgggatt atgtattggc ttagcgaaac
     3121 gagataaagg aaccgctgcg ttagcaggag taactggtta cttagttatg actgcaacga
     3181 tcaaagcttt ggtaaaactt tttatggcag aaggatctgc aattgatact ggagttattg
     3241 gagcattagt tgtcggaata gttgccgtat atttgcacaa ccgatataac aatattcaat
     3301 taccttccgc tttaggattc tttggaggtt cacgcttcgt tcctattgtt acatcgttct
     3361 cttctatctt gattggcttt gtcttctttg ttatttggcc acctttccaa caacttcttg
     3421 tttctacagg tggatatatt tctcaggcgg gtccaattgg aacttttcta tatggatttt
     3481 taatgagact ttctggagca gtaggcttac atcatataat ttaccctatg ttttggtata
     3541 ctgaacttgg tggtgttgaa actgttgcag gacaaacagt ggttggagct caaaaaatat
     3601 tttttgctca attagccgat ttggcccatt ctggattatt tacagaagga acaaggtttt
     3661 ttgcaggtcg tttctcaaca atgatgttcg gtttaccggc tgcctgttta gcgatgtacc
     3721 atagtgttcc taaaaatcgt cgtaaaaaat acgcgggttt gttttttgga gttgctttaa
     3781 catcttttat taccggtatt acagaaccaa ttgaatttat gtttctattc gtcagtccgg
     3841 ttctatatgt tgttcacgca ttccttgatg gtgttagctt ctttattgca gacgtcttaa
     3901 atatttcaat aggaaacaca ttttcaggag gtgtaatcga tttcacttta tttggaattt
     3961 tgcaggggaa cgctaagacg aattgggttc ttcagattcc atttggactt atttggagtg
     4021 ttttgtatta tattattttt agatggttca ttactcaatt caacgttcta acgccagggc
     4081 gaggagaaga agtagattct aaagaaattt ctgaatccgc agattcaact tcaaatactg
     4141 cagattattt aaaacaggat agcctacaaa ttatcagagc cttgggtgga tcaaataata
     4201 tagaagatgt agatgcttgt gtgacacgtt tacgtgtagc tgtaaaagaa gttaatcaag
     4261 ttgataaagc acttttaaaa caaattggtg cagttgatgt cttagaagtg aagggtggca
     4321 ttcaagcaat ctatggagca aaagcaatct tatataaaaa tagtattaat gaaattttag
     4381 gtgtagatga ttaagtactt actgacttaa taaaaaacag aggagagtga tggatgagta
     4441 ggatgaaatg aaatcgcata caagaaataa agaactcatt atccaagttg gatacgctta
     4501 ttacatagga gaatacaaat gaaatttaga aaattagctt gtacagtact tgcgggtgct
     4561 gcggttcttg gtcttgctgc ttgtggcaat tctggcggaa gtaaagatgc tgccaaatca
     4621 ggtggtgacg gtgccaaaac agaaatcact tggtgggcat tcccagtatt tacccaagaa
     4681 aaaactggtg acggtgttgg aacttatgaa aaatcaatca tcgaagcgtt tgaaaaagca
     4741 aacccagata taaaagtgaa attggaaacc atcgacttca agtcaggtcc tgaaaaaatc
     4801 acaacagcca tcgaagcagg aacagctcca gacgtactct ttgatgcacc aggacgtatc
     4861 atccaatacg gtaaaaacgg taaattggct gagttgaatg acctcttcac agatgaattt
     4921 gttaaagatg tcaacaatga aaacatcgta caagcaagta aagctggaga caaggcttat
     4981 atgtatccga ttagttctgc cccattctac atggcaatga acaagaaaat gttagaagat
     5041 gctggagtag caaaccttgt aaaagaaggt tggacaactg atgattttga aaaagtattg
     5101 aaagcactta aagacaaggg ttacacacca ggttcattgt tcagttctgg tcaaggggga
     5161 gaccaaggaa cacgtgcctt tatctctaac ctttatagcg gttctgtaac agatgaaaaa
     5221 gttagcaaat atacaactga tgatcctaaa ttcgtcaaag gtcttgaaaa agcaactagc
     5281 tggattaaag acaatttgat caataatggt tcacaatttg acggtggggc agatatccaa
     5341 aactttgcca acggtcaaac atcttacaca atcctttggg caccagctca aaatggtatc
     5401 caagctaaac ttttagaagc aagtaaggta gaagtggtag aagtaccatt cccatcagac
     5461 gaaggtaagc cagctcttga gtaccttgta aacgggtttg cagtattcaa caataaagac
     5521 gacaagaaag tcgctgcatc taagaaattc atccagttta tcgcagatga caaggagtgg
     5581 ggacctaaag acgtagttcg tacaggtgct ttcccagtcc gtacttcatt tggaaaactt
     5641 tatgaagaca aacgcatgga aacaatcagc ggctggactc aatactactc accatactac
     5701 aacactattg atggatttgc tgaaatgaga acactttggt tcccaatgtt gcaatctgta
     5761 tcaaatggtg acgaaaaacc agcagatgct ttgaaagcct tcactgaaaa agcgaacgaa
     5821 acaatcaaaa aagctatgaa acaatagtcc ttagttattc tataaaaagt agttttttaa
     5881 agaacctaag agtgtatacc cccttttccc tctacacaga tagtgtaaga aaagggggct
     5941 tttgtttaaa atgtaagaaa ctgtcacgaa attaaaatga agttcttaca taagcgaatc
     6001 ataaaaaatt tcattttgat tttaaaacag ttcaagaaag tcaaaaaatt attctatttg
     6061 aaagagaggt gccgactgtg aaagtcaata aaatccgtat gcgggaaaca gtgatttcct
     6121 acgctttcct agcaccagta ttattcttct ttgtcatctt tgtgttggct ccgatggtga
     6181 tgggcttcat tacaagtttc tttaactact caatgactaa atttgagttt gtaggcttgg
     6241 ataactatat ccgtatgttt aaagatcctg tctttacaaa atctctgatt aacacagtta
     6301 ttttggttat tggatctgta ccagttgttg ttctattctc actctttgta gcatctcaga
     6361 cctatcatca aaatgtcatt gccagatcct tctaccgttt cgtcttcttc cttcctgttg
     6421 taacgggtag tgttgccgtg acagttgttt ggaaatggat ttatgaccca ctatcaggga
     6481 ttctaaactt tgtccttaag tccagccaca tcatcagcca aaacatttct tggttgggag
     6541 ataaaaactg ggcattgatg gcgattatga ttattctctt gaccacttca gttggtcagc
     6601 ccatcatcct ttatatcgct gccatgggga atattgacaa ttcactggtt gaagcggcgc
     6661 gtgttgatgg tgcaactgag tttcaagttt tttggaagat taaatggcca agccttcttc
     6721 caacaactct ttatattgca atcatcacaa caattaactc attccagtgt ttcgccttga
     6781 ttcagctttt gacatctggt ggtccaaact actcaacaag taccttgatg tactaccttt
     6841 acgaaaaagc cttccaattg acagaatacg gctatgccaa cacaattggt gtcttcttgg
     6901 cagtcatgat tgctatcgta agctttgttc aatttaaagt acttggaaac gacgtagaat
     6961 actaaagaaa ggagacagct atgcaatcta cagaaaaaaa accattaaca gcctttactg
     7021 ttatttcaac aatcattttg ctcttgttga ctgtgctgtt catctttcca ttctactgga
     7081 ttttgacagg ggcattcaaa tcacaacctg atacaattgt tattcctcct cagtggttcc
     7141 ctaaaatgcc aaccatggaa aacttccaac aactcatggt gcagaaccct gccttgcaat
     7201 ggatgtggaa ctcagtattt atctcattgg taaccatgtt cttagtttgt gcaacctcat
     7261 ctctagcagg ttatgtattg gctaaaaaac gtttctatgg tcaacgcatt ctatttgcta
     7321 tctttatcgc tgctatggcg cttccaaaac aagttgtcct tgtaccattg gtacgtatcg
     7381 tcaacttcat gggaatccac gatactctct gggcagttat cttgcctttg attggatggc
     7441 cattcggtgt cttcctcatg aaacagttca gtgaaaatat ccctacagag ttgcttgaat
     7501 cagctaaaat cgacggttgt ggtgagattc gtaccttctg gagtgtagcc ttcccgattg
     7561 tgaaaccagg gtttgcagcc cttgcaatct ttaccttcat caatacttgg aatgactact
     7621 tcatgcaatt ggtaatgttg acttcacgta acaatttgac catctcactt ggggttgcga
     7681 ccatgcaggc tgaaatggca accaactatg gtttgattat ggcaggagct gcccttgctg
     7741 ctgttccaat cgtcacagtc ttcctagtct tccaaaaatc cttcacacag ggtattacta
     7801 tgggagcggt caaaggataa tactctgcga aaatcgaatg caaactacgt cagcttcacc
     7861 ttgccatact taagtattgc ctgcggttag cttcctagtt tgttcttcaa ttttcattga
     7921 gtataggaaa atcaatctat caagatacag aagtatattt tatagattta gagaatatag
     7981 aggttataag tgtctacaaa atggagggta tgcagttact ttatgaagtt ttgtcagacg
     8041 cttatcaact taagaatggt tttagttaac tatcagaaac gaaggaaaga gtatgatttt
     8101 tgacgatttg aaaaacatca ccttttacaa agggattcat cccaatttag acaaggctat
     8161 cgactacctc taccaacatc gtaaagattc attcgaatta ggaaagtatg agattgatgg
     8221 agataaagtc tttctagttg ttcaggaaaa tgtcctcaat caagttgaga ataatcaatt
     8281 tgaacaccat aagaactatg cagatttgca tttgctggta gaaggacatg aatattcgag
     8341 ctacggttca cgtatcaaag acgaggcagt agcattcgac gaagcgactg acattggctt
     8401 tgttcattgt catgaacact acccactctt gttgggttat cataattttg cgattttctt
     8461 cccaggtgag ccacatcagc caactggcta tgcaggtatg gaagaaaagg ttcgaaaata
     8521 tctctttaaa attttgattg attaaaaata ggatgaattg tttttttgta aagctttgac
     8581 aatactctac catgaaattg atctttgtga ggtagagaaa tgagaataaa atatttaaaa
     8641 attggtatct tctaagcatg ctgcaagagc tagtttctta gatggacagg ggattacagt
     8701 tgatgagatg tcctggataa ttaatggcat tgtgaatgca ttgattggta gatacataaa
     8761 attaggtact tatgcagcta agtatggtat tagtatagca cgctcgatct taagtagggc
     8821 agctgcaact gcagcagcaa gagtaggatt actgaccaag atttctggat ggattttacg
     8881 agtagctgtg aatgtagctg atgtatatgg taattttgcc aacaatattg ctgcagcttg
     8941 ggatgcatat gataaaattc ctaacaatgg tcgtataaac ttttaaaatg cgagaatgaa
     9001 agcactttgt atttttttat tgaatatgtt agcttggaca gtgcttgcaa tgataattcg
     9061 tggagggcta gatggatttg ataggcatac ttggagtact attttaattg cttcgctgtt
     9121 cggggtatat gattataagc ccatagataa aaatagaaaa aagtccaaaa gaaaaaagaa
     9181 aaaatagatt tgttcatggt agggacttat gaaagcttta ctgacaaaaa agaaaacagt
     9241 ttacaaagaa aaatgatgga ggagcaaaca tggcacaaaa aggagtaagc cttatcaagg
     9301 cagcatttga tacagataac tttctcatgc gttttagtga gaaggtcttg gacatcgtga
     9361 cagccaatct tctttttgtc gtctcttgtt tgcccatcgt gacgattgga gtggctaaaa
     9421 tcagcctcta cgagaccatg ttcgaagtta agaagagcag acgggtgcct gtttttaaaa
     9481 tctatctaag atctttcaag caaaatctga aactaggtct tcagttaggt ctgcttgagt
     9541 tgggcattgt ttcattaagc ctcctagatc tttatctctt ttggggtcaa acagctctgc
     9601 cctttcaatt gctgaaagct atttgtctag gcattctgat tttccttacg attgtgatgt
     9661 tggctagcta tccaatcgcg gcacgttatg atttaacttg gaaagaaatt cttcaaaaag
     9721 gattgatgtt ggctagtttt aactttcctt ggttcttcct catgttagcc attcttgtcc
     9781 tcattgtgat ggttctttat ctgtctgcct tcagtctact cttaggtggc tcagtcttcc
     9841 tgctttttgg gtttggacta ttggtcttta tccagactgg attgatggag aaaattttcg
     9901 caaaatacca ataggagcct tatttctgaa actactttca aaggctccaa acgctattct
     9961 ataagcgaga aactaaaatc taagtgtcta taagatattg aaagcctttt cggtaaagtg
    10021 tatactaaag aagtaaagaa aaatctgctt tagcagaaaa taaaaataat aggagaaaat
    10081 ctatgtcaga tttgaaaaaa tacgaaggtg tcattccagc cttctacgca tgttatgatg
    10141 agcaaggaga agtaagccca gaacgtacgc gtgccttggt tcaatacttc attgataaag
    10201 gtgttcaagg tctttatgtc aatggttctt ctggtgaatg tatctaccaa agtgttgaag
    10261 accgcaagtt aatcttggaa gaagttatgg cagttgccaa aggtaaattg accattattg
    10321 cccatgttgc ttgcaataat actaaagata gtatggaact tgctcgccat gctgaaagct
    10381 tgggagtaga tgctattgca acgattccac caatttattt ccgcttgcca gaatactcag
    10441 ttgccaaata ctggaacgat atcagttctg cagctccaaa cacagactac gtgatttaca
    10501 acattcctca attggcaggg gttgctttga ctccaagcct ttacacagaa atgttgaaaa
    10561 atcctcgtgt tatcggtgtg aagaactctt ctatgccagt tcaagatatc caaacctttg
    10621 tcagccttgg tggagaagac catatcgtct ttaatggtcc tgatgagcag ttcctaggag
    10681 gacgcctcat gggggctagg gctggtatcg gtggtactta tggtgctatg ccagaactct
    10741 tcttgaaact caatcagttg attgcggata aggacctaga cacagcgcgt gaattgcagt
    10801 atgctatcaa cgcaatcatt ggtaaactca cttctgctca tggaaatatg tacggtgtca
    10861 tcaaagaagt cttgaaaatc aatgaaggct tgaatattgg atctgttcgt tcaccattga
    10921 caccagtgac tgaagaagat cgtccagttg tagaagcggc tgctgccttg attcgtgaaa
    10981 ccaaggagcg cttcctctaa tctaaaagga ggtatttatg acatattacg ttgcaattga
    11041 tatcggtgga accaacatca agtatggttt ggttgatcaa gaggggcaac ttcttgaatc
    11101 gcatgaaatg ccaactgagg cgcataaggg tggacctcat atcttacaaa agaccaaaga
    11161 tatcgtagct agttatttag aaaaaggccc agtagcaggt gttgccatat cttctgctgg
    11221 gatggtggat ccggataagg gtgagatttt ctatgctggg ccgcaaatcc ctaactacgc
    11281 aggcacccag ttcaaaaagg aaatcgaaga aagctttact attccttgtg agattgaaaa
    11341 tgatgtcaac tgtgcaggtc ttgctgaggc agtatctggt tcaggcaagg gagcaagtgt
    11401 gacactttgc ttgaccattg gaacaggtat cggtggttgc ttgattatgg ataggaaagt
    11461 cttccatggt tttagcaatt cagcctgtga agtcgggtat atgcatatgc aggatggagc
    11521 ttttcaagac ttggcttcta caacagcttt agtgaaatat gtagctgaag cccatggaga
    11581 agatgttgat cagtggaatg gccgtagaat tttcaaagaa gccactgaag gaaacaaaat
    11641 ctgcatggaa ggtattgacc gtatggttga ctatctagga aaaggtctgg caaatatttg
    11701 ctatgttgcc aatccagaag tggttattct tggtggtggt atcatggggc aagaggctat
    11761 cctcaaacct aagatccgca cagccttgaa agaggctttg gtaccaagtt tagcagaaaa
    11821 aacacgatta gaatttgccc atcaccaaaa tacagcaggg atgttgggtg catattatca
    11881 ttttaagaca aaacaatcct agtttggctc agccaaacta ggattttctt acacgttttt
    11941 gtctacgata gccgttgagt tttttatttt cccagtagct attaaagatt ttttccttgc
    12001 tttcgcgatt gatttccaaa aagtaggcat aaatcaaatc gataaagaag agcataggaa
    12061 gttgagcgga tattcgttgg atataggagg gttggctgtg ggtggctaca agaacagtct
    12121 ctgtataggt ctggctatct ttattgggaa cacttgtaaa gagtacagtc tttgccccca
    12181 tctccttggc atctaataga ctatctaaaa tagaaggagt tgagcctgaa agtgagaagc
    12241 ccagtactag acaattttca tccatgatgc tggttgtcca ggcaaagccg tcttggtctg
    12301 tcaaagcttc gcagaccaca cctagtcgca taaaacgtaa tttcatttca cgggcgacga
    12361 ggccagaact ccctgttcca aagaagtaga tacgctcagc atcttcgatt agctgggcaa
    12421 ttcgctctag ttggatttcg tcaatcaagt cttgtgtttg ttccctcata ttgctataac
    12481 ttctgaggac tcgtttggtc agtggactgt gcttggagac ttggttggct tgattttctg
    12541 cctgatgttg gtattggaaa ataaattctc ggtagccagt aaagccacac tttttagcaa
    12601 agcgggtcaa agcagcttga gaaatatgta atttttgggt gacttgttga gaagataaat
    12661 catctgtaat cgtttcagct tgcaaaaaat agcgagcgat ttcttgctct aggtctgtca
    12721 tttcttcaaa atgtgaatca atgatagttg cgatatctgg tttgtccata gggaaagctc
    12781 ctttacatga gtcatactgg aagactagat cagggaatag tcacacttca ttataacaca
    12841 taatataagg atagataaat aaaaacgcat ctctgtttta aaaacgaaaa aatcgaaaaa
    12901 gcttctctct tttccataat tttctactca aattgtggta caattaagag taagatttta
    12961 agttagaaat gagactgatt tgtatgagaa aatttaacag ccattcgatt ccgattcggc
    13021 ttaatttatt gttttcaatc gtcattttac tctttatgac cattattggt cgtttgttgt
    13081 atatgcaggt tttgaacaag gatttttacg aaaaaaagct agcctcagct agtcagacca
    13141 agattacaag cagttcagcc cgtggggaaa tttatgatgc tagtggaaaa cctttggtag
    13201 aaaatacgtt aaagcaggtt gtttccttta cgcgtagcaa taaaatgacg gctacagact
    13261 taaaagaaac agctaaaaag ttactgactt atgtgagcat cagttctcca aatttgacag
    13321 aacgccagct ggcggattac tatttggctg atcctgaaat ctataaaaaa atagtggaag
    13381 ctctcccaag tgagaaacgc ttggattcag atggcaatcg tctatccgaa tcagaactgt
    13441 ataacaatgc ggtcgatagt gtccaaacaa gtcaactaaa ctatacagag gatgaaaaga
    13501 aagaaatcta tctttttagt cagttaaatg ctgttggaaa ctttgcgaca ggaaccattg
    13561 ctacagatcc tctaaatgat tctcaggtgg ctgttattgc ctctatttca aaggagatgc
    13621 ctggcattag tatttctact tcttgggata gaaaggtttt ggaaacttcc ctttcttcta
    13681 tagttgggag tgtatccagt gaaaaagctg gtctcccagc ggaagaagca gaagcctatc
    13741 ttaaaaaagg ctattctcta aatgatcgtg taggaacctc ctatttggaa aagcaatatg
    13801 aagagacctt acaaggaaaa cgctcggtaa aagaaatcca tctggataaa tatggcaata
    13861 tggaaagcgt ggatacaatt gaggaaggta gtaagggaaa caatatcaaa ctgaccattg
    13921 atttggcctt ccaagatagc gtggatgctt tactgaaaag ttatttcaat tccgagctag
    13981 aaaatggtgg agccaagtat tctgaaggtg tctatgcagt cgcccttaac ccaaaaacag
    14041 gtgcggtttt gtctatgtca gggattaaac atgacttgaa aacgggagag ttgacgcctg
    14101 attccttggg aacggtaacc aatgtctttg ttccaggttc ggttgtcaag gcggcgacca
    14161 tcagctcagg ttgggaaaat ggagtcttgt caggaaacca gaccttgaca gaccggtcca
    14221 ttgtcttcca aggttcagct cccatcaatt cttggtatac tcaggcttac ggttcattcc
    14281 ctatcacagc ggtccaagct ctggagtatt catcaaatac ctatatggtc caaacagcct
    14341 taggtcttat ggggcaaacc tatcaaccca atatgtttgt cggcaccagc aatctagagt
    14401 ctgctatgga gaaactgcgt tcaacctttg gcgaatatgg cttgggtact gcgacaggaa
    14461 ttgacctacc agatgaatct actggatttg ttcccaaaga gtatagcttt gctaattaca
    14521 ttactaatgc ctttgggcag tttgataact atacgccgat gcagttggct cagtatgtag
    14581 caactattgc aaataatggt gttcgtgtgg ctcctcgtat tgttgaaggc atttatggta
    14641 ataatgataa gggaggactg ggtgacttga ttcagcaact gcaaccgaca gagatgaata
    14701 aggtcaatat atccgactcc gatatgagca tcttgcacca aggtttttat caggttgccc
    14761 atggtactag tggattgaca actggacgtg ccttttcaaa tggtgccttg gtatccatta
    14821 gcggaaaaac aggtacagcc gaaagctatg tggcagatgg tcagcaagca accaatacca
    14881 atgcggtggc ctatgcccca tctgataatc cccaaatcgc tgtcgcagtg gtctttcctc
    14941 ataataccaa tctaacaaat ggtgtaggac cttccattgc gcgtgacatt atcaatctgt
    15001 atcaaaaata ccatccaatg aactagaaag gaaattatgc tttatccaac acctattgcc
    15061 aagttgattg acagttattc taagttacca ggtatcggga ttaagacggc tacgcgtctg
    15121 gccttttata cgattgggat gtctgctgat gatgtcaatg aatttgcaaa aaatctcctt
    15181 tctgctaaga gagaattgac atattgttct atttgtggac gtttgacaga cgacgatcct
    15241 tgttctatct gtactgatcc gactcgtgac cagacaacaa ttttagttct tgaggatagt
    15301 agagatgtgg cagccatgga aaatatccaa gaataccatg gactctatca tgtccttcat
    15361 ggcctcattt ctcctatgaa tggtatcagt ccggacgata tcaatctcaa gagccttatg
    15421 actcgtctta tggatagtga gatttcagaa gtgattgtgg cgactaatgc tacagcggat
    15481 ggtgaagcga cttccatgta tctttcacgt ttgctcaagc cggctggtat caaggttacg
    15541 cgtctagcac gaggtctcgc tgtgggagcg gacattgagt atgcggacga agtgacactc
    15601 ttacgagcga ttgaaaatcg gacagagttg taagtgtagg caaatttacg aactccattc
    15661 atttataaaa aatcaaagag gctgaaaatc gttcctatcg gcctcttttt gtatagtgtg
    15721 atgagtagaa tcaggttcaa gttttaaaaa accaagcaaa tatgatatac taaagagcga
    15781 gtattctagt agaattagga caaataatat gaaacaaacg attattcttt tatatggtgg
    15841 acggagtgcg gaacgcgaag tctctgtcct ttcagctgag agtgtcatgc gtgcggtcaa
    15901 ttacgaccgt ttcacagtca agactttctt tatcagtcag tcaggtgact ttatcaaaac
    15961 gcaggaattt agtcatgctc cggggcaaga agaccgtctc atgaccaatg aaaccattga
    16021 ttgggataag aaagttgcac caagtgctat ctacgaagaa ggtgcagtgg tctttccagt
    16081 ccttcacggg ccaatgggag aagatggctc tgttcaagga ttcttggaag ttttgaaaat
    16141 gccttacgtt ggttgcaaca ttttgtcatc aagtcttgcc atggataaaa tcacgactaa
    16201 gcgtgttctg gaatctgctg gtattgccca agttccttat gtggctatcg ttgaaggcga
    16261 tgatgtgact gctaaaatcg ctgaagtgga agaaaaattg gcttatccag tcttcactaa
    16321 gccgtcaaac atggggtcta gtgtcggtat ttctaagtct gaaaaccaag aagaactccg
    16381 tcaagcctta gaacttgcct tccgatatga tagccgtgtc ttggttgagc aaggagtgaa
    16441 tgcccgtgaa attgaggttg gcctcttggg taactacgat gtcaagagca cgctaccagg
    16501 agaagttgtc aaggacgttg ccttttatga ctacgatgcc aagtatattg ataacaagat
    16561 tactatggat attcctgcca aaatcagtga tgatgtggtg gctgtcatgc gtcaaaatgc
    16621 agaaacagcc ttccgtgcca ttggtggcct tggtctatct cgttgcgatt tcttctatac
    16681 agataaggga gagatttttc tcaacgagct caataccatg ccaggtttta cccagtggtc
    16741 tatgtaccca ctactttggg acaatatggg gatcagctac ccagaactaa tcgagcgttt
    16801 ggttgacctt gccaaggaaa gttttgacaa gcgcgaagcg catttgatat aaaatgaaag
    16861 agagggtaga agccagaacc atcactgcaa ggtgactaga gttctcggac ttcagccctt
    16921 tttaaaggag tagaaatgaa attaacaatc catgaaattg cccaagttgt tggagccaaa
    16981 aatgatatca gtatctttga ggacacccag ttagaaaaag ctgagtttga tagtcgtttg
    17041 attggaactg gagatttatt tgtgccactt aaaggtgcgc gtgatggcca tgactttatt
    17101 gaaacagcct ttgaaaatgg tgcagcagta accttgtctg agaaagaggt ctcaaatcat
    17161 ccttacattc tagtagatga tgttttgaca gcctttcaat ccttagcatc ctactatctt
    17221 gaaaaaacga ctgttgatgt ctttgctgtt acaggttcaa atggcaagac aacgactaag
    17281 gatatgttgg cgcatttact gtcaacaaga tacaagacct acaaaacaca aggcaattac
    17341 aataatgaga ttggccttcc ttacacagtt cttcatatgc ctgaaggaac agaaaagttg
    17401 gttttggaga tgggacagga tcacttggga gatattcatc tcttgtctga attggctcgt
    17461 ccaaaaacag ccatcgtgac cttggttgga gaagctcatt tagccttttt caaagatcgt
    17521 tctgagattg ctaaaggaaa aatgcaaatt gcagacggaa tggcttcagg ttccttgctt
    17581 ttagcgccgg ctgaccctat cgtagaggac tatttgccaa ctgataaaaa ggtggttcgt
    17641 tttgggcaag gggtagagct ggaaattact gacttggttg agcgcaaaga tagtctgacc
    17701 ttcaaggcca atttcttaga gcaagtcctt gatttgccag taactggcaa gtacaatgcg
    17761 acaaatgcta tgattgcatc ctatgttgcc ttgcaagaag gagtttcaga ggagcaaatt
    17821 cgtttggcct tccaagatct tgaattgacg cgtaaccgta ccgagtggaa gaaagcagcc
    17881 aatggagcag atatcctgtc agatgtttac aatgccaatc caactgctat gaaactgatt
    17941 ttagagactt tctctgccat tccagccaat gaaggtggca agaaaattgc agttttggcg
    18001 gatatgaagg agcttggtga ccagtctgtt caacttcata atcagatgat tttgagcctc
    18061 tccccagatg tgcttgatat cgttattttc tatggagaag acattgctga attagcccaa
    18121 ttggccagtc aaatgttccc aattggccac gtttactact tcaagaaaac agaagaccaa
    18181 gaccaatttg aagacttagt caagcaggtc aaggaaagcc tcagtgccaa tgatcaaatc
    18241 ttgctcaagg gctctaactc tatgaaccta gccatgttgg tagaaagttt agaaaatgaa
    18301 accaagtgat tttgttaagt atctgtaaag aatgattgcc cttacagata ctggcttaac
    18361 ctttacaaaa gagccttttg accgtgagcg ctacgaagac ttgcgaagtc tgttatctga
    18421 aatgttgaat caggtatcag accttgatgc agacgaagtg gcagaactct tgaaaccgac
    18481 ctccgcttat gcgactccct taatggacgt ccgtgcttgg attgttgagg atgagaagat
    18541 ttgtctggtt aggggacaag gagaggatag ttgggctttg ccaggtggct ttggtgaagt
    18601 cggctattct ccaactgaaa atattctcaa ggaaattgaa gaagaaaccg gttttaaagc
    18661 caaagttgaa agactgctag ctgtttttga taccaatcgt ttccaactac agagcaaaca
    18721 atatgcaaag tttgtttttg aatgtaagct tctcgatgga caattccaag aaaatcaaga
    18781 aatcgctgac cttcaatttt ttgcaattga ccaactgccg aatttatctg aaaaacgcat
    18841 taccaaggag caaatcgaga ttctttggca ggtttatcaa ggtcagaggg agcaatatct
    18901 tgactaagaa gatgattatc gtatttctaa attcattttt cttaagtagc atggtataat
    18961 aaaatgcagg aaaattttga atcatgagga agactagatg aatttatggg atattttctt
    19021 tacgactcag gcaaccgagc cgcccaaatt tgaccttttt tggtatgtta gcctatttac
    19081 gctcttagcc ttaacctttt atacagccca tcgctatcgt gaaaagaagg tttaccaacg
    19141 atttttccaa atcttgcaga ctgttcagtt aatccttctt tatggttggt actgggtcaa
    19201 tcatatgcca ctgtcagaaa gcctaccctt ttaccattgc cgtatggcta tgtttgtggt
    19261 actcttgctt cctggtcagt ccaaatataa acaatacttt gcattattgg gaacatttgg
    19321 gacattagca gcctttgttt atccagtgcc agatgcttac ccttttccac atatcaccat
    19381 tctatccttt atctttggtc atttagcact cttggggaac tctctagttt atctattgag
    19441 acagtataat gcgcgattgc tggatgtgaa gggaattttt ctcatgacct ttgccctaaa
    19501 tgccttgatt tttgtggtca atttggtgac aggtggcgat tacggatttt tgacaaaacc
    19561 gccattggtt ggggatcacg gtctagtagc taattattta cttgtttcaa ttgtgctggt
    19621 agctactatc agtttgacta agaaaatctt agaattcttt ttagctcaag aagcagaaaa
    19681 aatgattgca aaggaagctt aacacagagc tttctttttt gctcttagag agtttttaca
    19741 agcagcttat aaaataagaa tttctgaata gacaaactca aaaaatggct gggaaattta
    19801 ggaaaaaagc aagcacgatt aaattttttg tgttataata ttttgtgaat agctatgcct
    19861 atgtttagct atggaataat acgaagtgcg aaacttggaa gatagagagg aagcgatgta
    19921 atggctagag aaggcttttt tacaggtcta gatattggaa caagctctgt caaggtgctt
    19981 gtggccgagc agagaaatgg tgaattaaat gtaattggcg tgagtaatgc caaaagtaaa
    20041 ggtgtaaagg atggaattat tgttgatatt gatgcagcag caactgctat caagtcagcc
    20101 atttcccaag cggaagaaaa ggcaggcatt tcgattaaat cagtaaatgt cggcttgcct
    20161 ggtaatcttt tgcaggtaga accaactcag gggatgattc cagtaacatc tgatactaag
    20221 gaaattacgg atcaagatgt tgaaaatgtt gtcaaatcag ctttgacaaa gagtatgaca
    20281 cctgaccgtg aagtcattac ctttattcct gaagaattta ttgtggatgg tttccaaggg
    20341 attcgtgacc cacgtggcat gatgggggtt cgccttgaaa tgcgtggttt gctttataca
    20401 ggacctcgta ctatcttgca caatttgcgt aagacggttg agcgtgcagg tgttcaggtt
    20461 gaaaatgtta tcatttcacc actagcaatg gttcagtctg ttttgaacga aggggaacgt
    20521 gaatttggtg ctacagtgat tgatatgggg gcaggtcaaa cgactgtcgc tacaatccgt
    20581 aatcaagaac tccagttcac acatattctc caagaaggtg gagattatgt aactaaagat
    20641 atctccaagg ttttgaaaac ctctcgcaaa ttagcggaag gcttgaaact gaattacggg
    20701 gaagcctatc cgcctcttgc aagcaaagaa accttccaag tagaggttat tggagaagta
    20761 gaagcagtcg aagtgacgga agcctacttg tcagaaatta tttctgcacg aatcaagcac
    20821 atccttgaac aaatcaagca agaattagat agaaggcgtc tattggacct ccctggtggt
    20881 attgtcttaa tcggtgggaa tgccatttta ccaggtatgg ttgagcttgc tcaggaagtc
    20941 tttggcgtcc gtgtcaagct ttatgttcca aatcaagttg gtatccgtaa tccagccttt
    21001 gcgcatgtga ttagtttatc agaatttgcg ggtcaattaa cagaagttaa tcttttggct
    21061 cagggagcga taaaaggtga gaatgactta agtcatcagc caattagttt tggtgggatg
    21121 ctgcaaaaaa cagctcagtt tgtacaatca acgcctgttc aaccagctcc tgctccagaa
    21181 gtagagccgg tggcgcctac agaaccaatg gcggatttcc aacaagcttc acaaaataaa
    21241 ccgaaattag cagatcgttt ccgtggattg atcggaagca tgtttgacga ataaagagga
    21301 aaaataaatt atgacatttt catttgatac agctgctgct caaggggcag tgattaaagt
    21361 aattggtgtc ggtggaggtg gtggcaatgc catcaaccgt atggtcgacg aaggtgttac
    21421 aggcgtagaa tttatcgcag caaacacaga tgtacaagca ttgagtagta caaaagctga
    21481 gactgttatt cagttgggac ctaaattgac tcgtggtttg ggtgcaggag gtcaacctga
    21541 ggttggtcgt aaagccgctg aagaaagcga agaaacactg acggaagcta ttagtggtgc
    21601 cgatatggtc ttcatcactg ctggtatggg aggaggctct ggaactggag ctgctcctgt
    21661 tattgctcgt atcgccaaag atttaggtgc gcttacagtt ggtgttgtaa cacgtccctt
    21721 tggttttgaa ggaagtaagc gtggacaatt tgctgtagaa ggaatcaatc aacttcgtga
    21781 gcatgtagac actctattga ttatctcaaa caacaatttg cttgaaattg ttgataagaa
    21841 aacaccgctt ttggaggctc ttagcgaagc ggataacgtt cttcgtcaag gtgttcaagg
    21901 gattaccgat ttgattacca atccaggatt gattaacctt gactttgccg atgtgaaaac
    21961 ggtaatggca aacaaaggga atgctcttat gggtattggt atcggtagtg gagaagaacg
    22021 tgtggtagaa gcggcacgta aggcaatcta ttcaccactt cttgaaacaa ctattgacgg
    22081 tgctgaggat gttatcgtca acgttactgg tggtcttgac ttaaccttga ttgaggcaga
    22141 agaggcttca caaattgtga accaggcagc aggtcaagga gtgaacatct ggctcggtac
    22201 ttcaattgat gaaagtatgc gtgatgaaat tcgtgtaaca gttgtcgcaa cgggtgttcg
    22261 tcaagaccgc gtagaaaagg ttgtggctcc acaagctaga tctgctacta actaccgtga
    22321 gacagtgaaa ccagctcatt cacatggctt tgatcgtcat tttgatatgg cagaaacagt
    22381 tgaattgcca aaacaaaatc cacgtcgttt ggaaccaact caggcatctg cttttggtga
    22441 ttgggatctt cgccgtgaat cgattgttcg tacaacagat tcagtcgttt ctccagtcga
    22501 gcgctttgaa gccccaattt cacaagatga agatgaattg gatacacctc catttttcaa
    22561 aaatcgttaa gtaaatgaat gtaaaagaaa atacagaact tgtttttcga gaagttgcag
    22621 aggctagtct gagtgctcat cgagagagtg gttcggtctc tgtcattgca gttaccaagt
    22681 atgtagatgt accgacagcg gaagccttgc ttccgctagg tgttcatcat atcggtgaaa
    22741 atcgtgtaga taagtttctg gaaaaatatg aagctttaaa agatcgagat gtgacttggc
    22801 atttgattgg taccttgcaa agacgtaagg tgaaagatgt cattcaatac gttgattatt
    22861 tccatgcatt ggactcagta aagctagcag gggaaattca aaaaagaagt gaccgagtca
    22921 tcaagtgttt ccttcaagta aatatttcta aagaagaaag caaacacggt ttttcgagag
    22981 aggaactgct ggaaatcttg ccagagttag ccagactaga taagattgaa tatgttggtt
    23041 taatgacgat ggcacctttt gaggctagca gtgagcagtt gaaagagatt ttcaaggcgg
    23101 cccaagattt acaaagagaa attcaagaga aacaaattcc aaatatgcct atgaccgagt
    23161 taagtatggg aatgagtcgt gattataaag aagcgattca attcggttcc acttttgttc
    23221 gtataggtac atcatttttt aagtaggaga gaaccatgtc tttaaaagat agattcgata
    23281 gatttataga ttattttacg gaggatgagg attcaagtct cccttatgaa aaaagagatg
    23341 agcctgtgtt tactccagta aattcttcac aggaaccggc tctcccaatg aatcaacctt
    23401 cacagtcggc tggtacaaaa gagaacaata tcaccagact tcatgcaaga caacaggaat
    23461 tggcaaatca gagtcagcgt gcaacggata aggtcattat agatgttcgt tatcctagaa
    23521 aatatgagga tgcaacagaa attgttgatt tattggcagg aaacgaaagt atcttgattg
    23581 attttcagta tatgacagag gtgcaggctc gtcgttgttt ggactatttg gatggagctt
    23641 gtcatgtttt agctggaaat ttgaaaaagg tagcttctac catgtatttg ttgacaccag
    23701 ttaatgttat tgtaaatgtt gaagatatcc gtttaccaga tgaagatcaa cagggtgagt
    23761 tcggttttga tatgaagcga aatagagtac gataatgatt tttttaattc gtatgattta
    23821 taatgcagtg gatatttact ccctgatttt ggtagccttc gctgtcatgt cttggtttcc
    23881 aggtgcctac gaatccagtt taggtcgttg gattgtagcg ttggtgaaac cagtacttgc
    23941 tcccttgcaa cgcctgcctt tacagatagc gggtcttgat ttatctgttt gggtcgcgat
    24001 tgttttggtt cgatttttag gagaaaacct agtgcgtttt ctggcgatga taggatgaat
    24061 aaagggattt atcagcattt ctccatagaa gatcgtccat ttcttgacaa gggaatggaa
    24121 tggataaaga aggtagaaga tagctatgct ccttttttaa ctccttttat caatcctcat
    24181 caggagaagc tattaaagat tttggccaaa acctatggtc ttgcttgtag cagtagtggg
    24241 gaattcgtct cgagtgagta tgttcgagtt ttattatacc cagattattt ccaaccagag
    24301 ttttcagatt ttgaaatatc tctccaagaa attgtgtatt ccaataaatt tgaacattta
    24361 acgcatgcta agattttagg gacagtcatc aatcaattag ggattgaacg gaaacttttt
    24421 ggagatatcc tagtagatga agaacgggcg cagattatga ttaatcagca gtttcttctt
    24481 ctctttcaag atggactaaa gaaaattggt cgtatacctg tttcgctgga ggaacgtcct
    24541 ttcaccgaga aaatagataa gctagaacag tatcgagaac tggatttatc tgtgtctagt
    24601 tttcgattag atgttctttt atcaaatgtt ttgaaactat ctaggaatca agcaaaccag
    24661 ttgattgaaa agaaacttgt ccaagtaaat tatcatgtgg tagacaaatc agattacact
    24721 gttcaagttg gagacttgat tagtgtgaga aaatttggtc gcttgagatt acttcaagat
    24781 aagggacaaa cgaaaaaaga gaagaaaaaa ataaccgtcc agttattatt aagtaagtga
    24841 ggaatagaat gccaattaca tcattagaaa taaaggacaa gacttttgga actcgattca
    24901 gaggttttga tccagaagaa gtcgatgaat ttttagatat tgtggttcgt gattacgaag
    24961 atcttgtgcg tgcgaatcat gataaaaatt tgcgtattaa gagtttagaa gagcgtttgt
    25021 cttactttga tgaaataaaa gattcattga gccagtctgt attgattgct caggatacag
    25081 ctgagagagt gaaacaggcg gcgcatgaac gttcaaacaa tatcattcat caagcagagc
    25141 aagatgcgca acgcttgttg gaagaagcta aatataaggc aaacgagatt cttcgtcaag
    25201 caactgataa tgctaagaaa gtcgctgttg aaacagaaga attgaagaac aagagccgtg
    25261 tcttccacca acgtctcaaa tctacaattg agagtcagtt ggctattgtt gaatcttcag
    25321 attgggaaga tattctccgt ccaacagcta cttatcttca aactagtgat gaagccttta
    25381 aagaagtggt tagcgaagta cttggagaac cgattccagc tccaattgaa gaagaaccaa
    25441 ttgatatgac acgtcagttc tctcaagcag aaatggaaga attacaagct cgtattgagg
    25501 tagccgataa agaattgtct gaatttgaag ctcagattaa acaggaagtg gaaactccaa
    25561 ctcctgtagt gagtcctcaa gttgaagaag agcctctgct catccagttg gcccaatgta
    25621 tgaagaacca gaagtagctc cagtgcatcc gacaggtcca acaccagcta cagaaactgt
    25681 tgattcagca ccaggatttg aagcaccgca agaatccgtt acaattttat aagaaacgat
    25741 gtgagaacaa tatcttatcc ttatatttcc agcgagcagg agatggtgtg agtcctgcat
    25801 tccctaacga taagattatc ctctcaaaaa ctcaagtctg aagctagtaa gatttgacgt
    25861 ttcccacgtt acgggataag agggagaaag actaaatctt tttccgaata aaggtggtac
    25921 cacgattttc gtcctttttg gaagtcgtgg tttttaattt gttattattt ataaaggaga
    25981 taccatgaaa ctcaaagata cccttaatct tgggaaaaca gctttcccaa tgcgtgcagg
    26041 ccttccgacc aaagagccag tttggcaaaa ggaatgggaa gatgcaaaac tttaccaacg
    26101 tcgtcaagaa ctaaatgaag ggaaaccgca ttttgtcttg catgatggcc ctccatacgc
    26161 aaacggaaat atccacgttg gacatgccat gaatcatatc tcaaaagata tcatcattcg
    26221 ttctaagtct atgtcaggtt ttaacgcacc ttatatccca ggttgggata ctcatggtct
    26281 gccaatcgag caagttttgg caaagcaagg tgtgaagcgc aaagaaatgg acttggttga
    26341 gtacttgaaa ctctgtcgtg agtacgctct ttctcaagtt tataaacaac gtgatgattt
    26401 caaacgcttg gggatgtcag gtgactggga aaatttatat gtgactttaa ctccagacta
    26461 tgaagcagcc caaatccgtg tctttggtga gatggctaat aagggttata tctaccgtgg
    26521 tgccaagcca gtttactggt catggtcatc tgagtcagcc cttgccgaag cggaaattga
    26581 ataccatgat ttggtttcga cttcacttta ttatgccaat aaggtaaaag atggcaaagg
    26641 tatcctagat acagatactt atatcgtagt ctggacaaca actccattta ccatcacagc
    26701 ttctcgtggt ttgactgttg gtgcagatat tgattacgtt ttggttcaac ctgctagtga
    26761 aactcgtaag tttgtcgttg ctgcagaatt gttgactagc ttgtctgaga aatttggctg
    26821 ggctgatgtt caagtgttgg caacttaccg tggtcaagaa ctcaaccaca tcgtaacaga
    26881 acacccatgg gatacagctg tagatgagtt ggtaatcctt ggtgatcacg ttaccactga
    26941 ctctggtaca ggtatcgtcc atacagcccc tggttttggt gaggacgact acaatgttgg
    27001 tattgctaat ggtcttgaag tcgcagtgac tgttgacgaa cgcggtatca tgatggcgaa
    27061 tgctggtcct gagtttgaag gtcagttcta cgacaaggtt gtaccaactg ttattgaaaa
    27121 acttggtaac ctccttctag cccaagaaga aatctctcac tcatacccat ttgactggcg
    27181 tactaagaaa ccaatcatct ggcgtgcagt tccacaatgg tttgcctcag tttcaaaatt
    27241 ccgccaagaa atcttggatg ctattgataa agttaaattc catacagaat ggggtaaagt
    27301 acgtctatat aatatgattc gtgaccgtgg tgactgggtt atctctcgtc aacgtgcttg
    27361 gggtgttcca cttccaatct tctatgcaga agacggtaca gctatcatga cagctgagac
    27421 tattgaacat gtggcgcaac tctttgaagt acatggttca agcatttggt gggaacgtga
    27481 tgccaaagac ctcttaccag aaggatttac acatccaggt tctccaaatg gggagttcaa
    27541 gaaagaaaca gatattatgg atgtatggtt tgactcaggt tcatcatgga atggtgtttt
    27601 ggtaaatcgt ccaaatctga cttatccagc tgacctttac cttgaaggtt ctgaccaata
    27661 ccgtggttgg ttcaactcat cactcattac atctgttgcc aaccatggcg tagcacctta
    27721 caaacaaatc ttgtcacaag gttttaccct tgatggtaaa ggtgagaaga tgtctaaatc
    27781 acttggaaat accattgctc caagtgatgt tgaaaaacaa tttggtgcag aaatcttgcg
    27841 tctctgggta acaagtgttg actcaagcaa tgacgtgcgt atctctatgg atattttgag
    27901 ccaagtttct gaaacttacc gtaagattcg taacactctt cgtttcttga ttgccaatac
    27961 atctgacttt aacccagctc aagatgtagt cgcttacgat gaacttcgtt cagttgataa
    28021 gtacatgact attcgcttta accagcttgt caagaccatt cgtgatgcct atgcggactt
    28081 tgaattcttg acaatctaca aggccttggt gaactttatc aacgttgact tgtcagcctt
    28141 ctaccttgat tttgccaaag atgttgttta cattgaaggt gccaaatcat tggaacgccg
    28201 tcaaatgcag actgttttct atgacattct tgtcaaaatc accaaactct tgacaccaat
    28261 ccttcctcac actgcggaag aaatctggtc atatcttgag tttgaagctg aagacttcgt
    28321 tcaattgtca gaattgccag aagctcaaac ttttgctaat caagaagaag tcttggatac
    28381 atgggcagcc ttcatggact tccgtggaca agctcaaaaa gccttggaag aagctcgtaa
    28441 tgcaaaagtt atcgggaaat cgcttgaagc acacttgaca gtttatccaa atgaagttgt
    28501 gaaaactcta ctcgaagcag taaacagcaa tgtggctcaa cttttgatcg tgtcagactt
    28561 gaccatcgca gaagggccag ctccagaagc tgcccttagc ttcgaagatg tagccttcac
    28621 agttgaacgc gctgcaggtc aagtatgtga ccgttgccgt cgtatcgacc caacaacagc
    28681 agaacgcagc taccaggcag tcatctgtga ccactgtgca agcatcgtag aagaaaactt
    28741 tgcggaagca gtcgcagaag gatttgaaga gaaataaggt tgaaaagtct aggcaaaatt
    28801 caatttgaga agaaaagaca actaatttta tagtctatta aacgcattgt atcacgtttt
    28861 tgaatacctg atatgatgcg ttttttattt attttaaaaa tttgcgaggt atgacttttt
    28921 atactcaaca agaatcaaag agaaacttag caagctaaca gtagtaagat aaaataggaa
    28981 tttgata
//
LOCUS       DAVLSL010000010        28038 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLSL010000010 DAVLSL010000000
VERSION     DAVLSL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.010"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            68..334
                     /locus_tag="VXN30_000334"
     CDS             68..334
                     /locus_tag="VXN30_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357009.1"
                     /translation="METVQIVRIKDVIIEKISANDEELERIFGCSKRQAGDMRREMKK
                     LPSQQKYLRNDGQLVTIKGFDAYLQYRGSQPWKKEMAKTVKMTR"
     gene            415..648
                     /locus_tag="VXN30_000335"
     CDS             415..648
                     /locus_tag="VXN30_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1357010.1"
                     /translation="MALYSETEGTNIAENIKLIRLSLGETLEQFAKRFGTSKAVVCNW
                     EHKRNAPKLPTMLKIADLAGVSVIDFLNKKIEV"
     gene            660..1130
                     /locus_tag="VXN30_000336"
     CDS             660..1130
                     /locus_tag="VXN30_000336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357011.1"
                     /translation="MENLDNQEITITRGELKQLISDAIRENGKVTSNTIFNKSTSILK
                     RKSELHLKYPILRKMVELSASIYHPDPYRFEFASINRFNQAPSFKRIGSANQLHEIAR
                     KFALLCFGVDTNTDLSASDEEKAVSIYNEVIEIFFNNYEKHLEDKFGGDKWQKH"
     gene            1115..1825
                     /locus_tag="VXN30_000337"
     CDS             1115..1825
                     /locus_tag="VXN30_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEV1357012.1"
                     /translation="MAETLRKKLLNIQRDLKAPKGQYNDFGKYSYRNAEDIMEALKPL
                     LVGNNTVCYCKDDYIEQIGERYYLVATIVFADVETDEEITVKARAREEENKKGMDGSQ
                     ITGAASSYARKYALSGLFLIDDNKDADSNEYHKQNDQQKLKQQGQQQQQGNQQAQGQN
                     QVRYIDNTQYQEINDLINDIAKIKGMPFDTLANYVLSEKLKGLQDFHRVQVGDYEVLK
                     NYLTEQLAKAKAKAKRGN"
     gene            1828..2820
                     /locus_tag="VXN30_000338"
     CDS             1828..2820
                     /locus_tag="VXN30_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV1357013.1"
                     /translation="MVKDVTNSLTEIKVYFQPAVINVDREAIEAQVATVIAQYSGREV
                     TVDNYKEVYEERTRFNKLIGGLDTQRKDFNRQINEPAKDFDKWVKEKVIKPIEAVTDA
                     MSAGLNAIDEHERLMRVDVVRATFEDKCMVAGIEKSTFADKYDEYSLKKYFKTGKYEL
                     KKTTLDEMDGLVLSEFDALEEYKANKQAIQEQAQEYDLPADSYIRHLEDGKSLVDILK
                     MMKTDRDAEIARKEQKEIQEKAKAERLEEIAQSAKKNANANIKAYDAETGEILEQGTI
                     TPEPQNNEREVAKFEPSEPLVKLVRLELHGGLEQWENTQEYFEDNFIGFETLED"
     gene            2842..3018
                     /locus_tag="VXN30_000339"
     CDS             2842..3018
                     /locus_tag="VXN30_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357014.1"
                     /translation="MRCFYVSGKIADLDLGSEINAENSFMAAIEFVKRYTDLLKFGSN
                     EIKVSEVEEVQNDK"
     gene            3008..3574
                     /gene="ssb"
                     /locus_tag="VXN30_000340"
     CDS             3008..3574
                     /gene="ssb"
                     /locus_tag="VXN30_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1357015.1"
                     /translation="MINNVVLVGRLTRDAELRYTQSNIAVATFTLAVNRPFKNEAGER
                     EADFINCVIWRQLAENLANWAKKGSLIGVTGVIQTRSYDNQQGQRVYVTEVVASNFQL
                     LESRNSQQNNQDNYGGYQQQGYSNQGSSFQNGNNTGNNFQNGNSYGQQGSFFEGNTTN
                     PVPDFTRDNNPFGRPTNPLDISDDDLPF"
     gene            3587..3835
                     /locus_tag="VXN30_000341"
     CDS             3587..3835
                     /locus_tag="VXN30_000341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357016.1"
                     /translation="MVGYRWELPLASAERNNTGYIHSNAKPHLFNIVTGWSHCKRYWQ
                     VPLWAEEIKFDGKEKYFCKRCLKKYKKLQGVANEQSKS"
     gene            3816..4094
                     /locus_tag="VXN30_000342"
     CDS             3816..4094
                     /locus_tag="VXN30_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357017.1"
                     /translation="MNKVKVDLQCPFCGFCKVLKIPSHRKGITCPSCQQSVFLSWSTG
                     VEGYVDEHGFYFHAYEPFNIRKINREFQDAFEDAPPRRPFTIRSKTKE"
     gene            4108..5346
                     /locus_tag="VXN30_000343"
     CDS             4108..5346
                     /locus_tag="VXN30_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1357018.1"
                     /translation="MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDT
                     KGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARF
                     ASILRPKYLFLENVKGLLNHENGVTFETIISTLDELGYDVEWQVLNSKDFGVPQNRER
                     VFIIGHLRGGSGRKVFPLSRENQSISNQSVVKIGNVNPSGNGMNGEVYQADGLAPTLT
                     TNKGEGQKIAIKSNTIKQFGVLQPNFNQCGVVYETDGIAPTIRAYQGGGLEPKIIQRG
                     HGYNKGGEHDIAPTLTRNSYHENNVLKITEATSQGYAEATIGDSVNLSHPNSKTRRGR
                     VGKKIANTLLTGESQGVVEPDFRIRKLTPRECWRLQGFPDWAFDKAQEVNSNSQLYKQ
                     AGNSVTVSVIAAIAQGLGGN"
     gene            5381..5584
                     /locus_tag="VXN30_000344"
     CDS             5381..5584
                     /locus_tag="VXN30_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357019.1"
                     /translation="MTKIEIVMVLTTLMSITWAAIVTIHTMQAIKKHKAKVDYYQKPQ
                     VQCEIARHVLKNRWYSDGGEVFR"
     gene            5612..5791
                     /locus_tag="VXN30_000345"
                     /pseudo
     CDS             5612..5791
                     /locus_tag="VXN30_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5808..6020
                     /locus_tag="VXN30_000346"
     CDS             5808..6020
                     /locus_tag="VXN30_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357020.1"
                     /translation="MSDYKQRMIEEYKQLKERTNKLSLMISNYYVGTLDFKLKCPIEL
                     LETQHYTMCAYLKILEQRAEIENIEF"
     gene            6031..6432
                     /locus_tag="VXN30_000347"
     CDS             6031..6432
                     /locus_tag="VXN30_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357021.1"
                     /translation="MKFEFSLPRNTKLKSLNMVINSNDRQHQTDKAKVTKRIRAFAYW
                     HTSMNKDKGRAAFSPSNPCEVTVTIYSPTKSKLDPPNLYPTVKAIIDGMTDAGIWTDD
                     NHKVIKKLSFVYGGLSEEKGHYRLEFDIEEV"
     gene            6434..6640
                     /locus_tag="VXN30_000348"
     CDS             6434..6640
                     /locus_tag="VXN30_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357022.1"
                     /translation="MIEVNIKFDNFEAHGFYQDDTKLGKIRDAIISQMNNGHVVILGE
                     DRSILLNPKVIKCVQFEVVEDDQI"
     gene            6690..7004
                     /locus_tag="VXN30_000349"
     CDS             6690..7004
                     /locus_tag="VXN30_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357023.1"
                     /translation="MEELKKKVNEVYNWTVEDGKPQPPKQDLPQVVKDRADYFWEMAE
                     DGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPGMSQVVIAVYLIYR
                     GN"
     gene            7006..7521
                     /locus_tag="VXN30_000350"
     CDS             7006..7521
                     /locus_tag="VXN30_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1357024.1"
                     /translation="MNIKALIKKYEELWNEHSPFYEPVPYTSMVELFLKELKQLDEPQ
                     KVKIPQFVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLNGYE
                     VEKEKRYLVKIKGNIKENMLVYGELLERYFFTKSFSLDDAIYSHTRKELENAKIGWVF
                     DCEGFEIEEVE"
     gene            7522..7734
                     /locus_tag="VXN30_000351"
     CDS             7522..7734
                     /locus_tag="VXN30_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357025.1"
                     /translation="MTKFVKIQSCYRGHTEDELINIDDIGRICLGPNILFLRTPYNLG
                     EHHISITQNSVDKLLKVLDIIGEDGK"
     gene            7731..8102
                     /locus_tag="VXN30_000352"
     CDS             7731..8102
                     /locus_tag="VXN30_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357026.1"
                     /translation="MRPCKYPFLGRRKKQETSSPLFSARPILNEVPIVEEVKVDLGVE
                     ANVGRSYPEMVIHLDISGYGNRVHSVHRFPGVFLTVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWNLFISDLVNEFVR"
     gene            8123..8416
                     /locus_tag="VXN30_000353"
     CDS             8123..8416
                     /locus_tag="VXN30_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357027.1"
                     /translation="MTQTLEQAAKAESKRIKIPAKIRPFDVGYRIINKHGQALALKNG
                     ASIFSLPSLAEKAIEKEFGKNDPNFDIGKHSVEEVAIVNLSKFHSYFEEVIHD"
     gene            8416..8808
                     /locus_tag="VXN30_000354"
     CDS             8416..8808
                     /locus_tag="VXN30_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357028.1"
                     /translation="MTKKKIERLSVIHRREINWLKWYFLRDKKNPKRTILEQKIIVSH
                     IKTDRLEAKFLSNLKKSTEDFIDKSDPKYLRAIKEVYVYENMNVIGACQKILFYSPTQ
                     AYVLLNAWFNDYFRATYTELLENAILDK"
     gene            8874..9968
                     /locus_tag="VXN30_000355"
     CDS             8874..9968
                     /locus_tag="VXN30_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4417 domain-containing protein"
                     /protein_id="HEV1357029.1"
                     /translation="MEMLQIEYVDIKSIKPYHKNARHNDGEATEKVAASIKAFGFQQP
                     ILVDDNNIIITGHTRLKAALSLGIDTIPIAHAVNLTDEQIKAYRLADNRVAEYSTWDS
                     ELLNIELSQFETIDMAQFGFELSVTGFNFGNEEEQQEETENEEEDAEDFHRDTTINQY
                     NLFNYDDTRVEGFYNMPKIEGVDHIPKDFQGFNYVLNKPDYSSCVHFFLDDYQFERIW
                     QRPDFYIEKLLEFDSALAPDFSLYLDMPIAMQVWNIYRSRLIGQIMQDYGLTVIPTVS
                     WASEESFDFCFDGLPKNSTLAISTIGVKQNKEQFEVWKNGVTEMIKRLTPKRIVVYGG
                     KVEYDYKDIEVVYFENATTERMKESGTKTN"
     gene            9949..10695
                     /locus_tag="VXN30_000356"
     CDS             9949..10695
                     /locus_tag="VXN30_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357030.1"
                     /translation="MAQKLTKLKDIFKHVSSIDLGKEILFEDLELYNKETETSKQYQS
                     IEEAENDLSLMEKVNKINFTLGGGRGANFEKGKDGKYPGFRGAGGARDSGSSKSLHPA
                     SLNNQGRFSSVEGTIQEFIKKHGGSRTEYSTAVDSQGFAHNYVHGGKNSVQILPISGG
                     FTAIHNHPNGSNFSSTDLHSFAALKGMNTLVATNSSKAYRITKGANFDAKGFDKAVSK
                     SRFTTKDYNKGADLWLKKNAKKYGYTYSYE"
     gene            10899..11354
                     /locus_tag="VXN30_000357"
     CDS             10899..11354
                     /locus_tag="VXN30_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein"
                     /protein_id="HEV1357031.1"
                     /translation="MPRDGTKNLKPVTERTKDEARAISSKGGKASGIARRKKADLKKA
                     FEILLSLDVTDSKIKKQLEEMGMAGNNEALLAFATFQQAVKGNQKATENIIKLTNTKD
                     KYDIQEQKERIKALKYENRERAEAEKGSSETIEIVDAWAEDVRGATDDL"
     gene            11344..12654
                     /locus_tag="VXN30_000358"
     CDS             11344..12654
                     /locus_tag="VXN30_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1357032.1"
                     /translation="MTFNVQKNINPHFKSVWISSLPYNVLKGGRNSFKSSVIVLKLAY
                     MMIRYIIAGEAANIVVIRKVANTIRDSVFNKVWWALNLFGIAEQFTKTVSPFKIVHKT
                     TGSTFYFYGQDDFQKLKSNDIGNIIAVWYEEAAEFNDQEDFDQSNVTFMRQKHPRAKF
                     VQFFWSYNPPRNPYSWINEWFESIKTNKNYLAHSSTYLDDELGFVTEQMLEDIERIKE
                     NDYDYYRYLYLGEAVGLGNNVYNMSMFHAIDALPSDDKLIGISFALDGGHQQSATACC
                     AFGITAKGKVILLDTWYYSPAGQVVKKAPSQLSKEIYTYIRSVIEKYRVQALQYTIDS
                     AEGALRNQMFLDFGLKWHPVAKLRKVTMIDSFQSLLAQGRFYYLNTENNKIFIEEHKM
                     YRWDEKTIKSDNPSVIKEDDHTCDTTQYFVLDNAKLLGLRVGNV"
     gene            12667..14235
                     /locus_tag="VXN30_000359"
     CDS             12667..14235
                     /locus_tag="VXN30_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1357033.1"
                     /translation="MSLFQKVKDFFSRGRYYMQTSNLNSILEHPKIAVTQEEYDRIKR
                     NLVYYQSKWDDVQYKNTDGDIKSRPMNHLPIARTASKKIASLVYNEQATITTKNEILQ
                     KFLDDMLTNDRFNKNFERYLESCLALGGLAMRPYIDGDKVRVAFIQAPVFFPLESNTQ
                     DVSSAAILTKTIKSEGRKNVYYTLVEFHEWVTADGQETGSTNDKKYYRITNELYRSDV
                     NDVLGQRVNLSELDKYKNLEPVTVFENLSRPLFTYLKTPGMNNKDINSPLGLSIFDNA
                     KTTIDFINRSYDEFMWEVRMGQRRVIVPEHLTQRQYQRPDGTIDFRPRFDVEQNVYMQ
                     IGGSSMDAGGITDLTSPIRANDYILAISEGLKLFEMQIGVSSGMFTFDGQGMKTATEI
                     VSENSDTYQMRSSIVALVEQSIKELCVSMCELGKAVGVYSGEIPELDDISVNLDDGVF
                     TDRHAELDYWAKMVAAGFSTKKRAIGKTLNISDVEAEKELNAINSELPPMNDAELAIY
                     GMHDQNEEEADDKG"
     gene            14222..14395
                     /locus_tag="VXN30_000360"
     CDS             14222..14395
                     /locus_tag="VXN30_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357034.1"
                     /translation="MTKVKFGVTSVDYSASIEDTPTIKLGLIIRGSGRLDASSVIKKL
                     IKDVSELEYELEE"
     gene            14438..15589
                     /locus_tag="VXN30_000361"
     CDS             14438..15589
                     /locus_tag="VXN30_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HEV1357035.1"
                     /translation="MKKKRKQITFNDQQFPLQMQGVGDIYEKLQIDIFDRMIKRLKER
                     GSIDLMRNPYIWQLEKLNDMHMLNEQNLKLISERTGIAERLLRDVIENEGLKVYKDTK
                     QQLEEDLNKIPEGEISNGVTDSLEAYSRQAVSDLNLINTTLPKSLQVAYKSIVEETVA
                     QVVAGTKTSDVALHDTIMKWQKNAFTGFVDKGGRHWKADSYARAIIKSTTYKVYNEMR
                     TRPAEELGVDTFYYSMKAMARPACSPLQGQIVTKGAGREIDGITIYSLLDYGYGTAAG
                     CLGIHCGHYLTPFIVGVHELPNLPDYLKNLTPEQAEENARIEAGQRGLERLIKTHKER
                     LHYAHTLQDDKMIQAERLKVRGYQTKIRNLINQHDFLTRDYRREKLYIS"
     gene            15728..16291
                     /locus_tag="VXN30_000362"
     CDS             15728..16291
                     /locus_tag="VXN30_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HEV1357036.1"
                     /translation="MAFTTEELLNLGLTEEQAKSVFALRGKELNEDKSALETIKQERD
                     SLKSQLQKAEEQVEHLKSLENISAEQKDAIDKLQAEYDKYKNEAAAELAQTKKVSAIS
                     LALKDTNAFNPDKLMKFIDVDAIQLDDNGKPQIDEVINGLKESDPYLFKAEESKPSPN
                     ILPQGNPAGEGTSDVDPFQAIIDGYGK"
     gene            16309..17190
                     /locus_tag="VXN30_000363"
     CDS             16309..17190
                     /locus_tag="VXN30_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001139822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEV1357037.1"
                     /translation="MPSNQNNAVRRYEKQYAGILETVFRVRAAFSNALAPIQILDGVQ
                     ENSKAFSVKTNNTPVVIGEYKTGENDGGFGDNSGAQSRFGGLTEVKYENTDVNYDYTL
                     TIHEGLDRYTVNNDLNAAVADRLKLQSEAQTRTVNKRIGKYLSDNATKTEALADFTDD
                     KVKALFNKLSAFYTNNEVTAPITVYLRSEFYNAIVDMASVTSAKGATISLDENGLPKY
                     KGFTLEETPAQYFETGVIAIFSPNGIVIPFVGISTARVIEAENFDGVKLQAAAKGGTY
                     TLDDNKKAICKVTGTIV"
     gene            17201..17434
                     /locus_tag="VXN30_000364"
     CDS             17201..17434
                     /locus_tag="VXN30_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357038.1"
                     /translation="MALYKATKNLFFEQLNMDVIVNDIIELDEDYAKEVNKKLKNAFP
                     DVKNVLELVDKNGTLEPEDAPSVDDASQATVED"
     gene            17477..17869
                     /locus_tag="VXN30_000365"
     CDS             17477..17869
                     /locus_tag="VXN30_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013794407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357039.1"
                     /translation="MTYLTQEEFDELGFDEVTDFEKLAKRAKIAIELYTNGIYQKDID
                     FEKEIAYRKSAVKLAMAFQIAYLDASGIMSADDKQLANSVSIGRTSISYSTSQSTLAG
                     QRFNLSMDAENALRQAGFSLVVGVAYDR"
     gene            17859..18230
                     /locus_tag="VXN30_000366"
     CDS             17859..18230
                     /locus_tag="VXN30_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEV1357040.1"
                     /translation="MIDKRLLKGIDKRLLKDVLTIKKVADKNDYGDEVYSEPLTIKNV
                     RFDRSVGRSGNRNSKTGTGNSKSRQKQGVIYLYPSLSFVTVDNSWMGAKVNDGIGDYT
                     INGFQTNYYDGEIFSQEIEVI"
     gene            18230..18574
                     /locus_tag="VXN30_000367"
     CDS             18230..18574
                     /locus_tag="VXN30_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEV1357041.1"
                     /translation="MSIAIKVDLQKAKQKLSNESMTRGKIAVASKILLDNEQYIPLRG
                     GELRASGRIVGQGDAVVYGTVYARAQFYGANGIVTFRRYTTPGTGKRWDQVATSKHAE
                     EWARAFVKGMGL"
     gene            18574..18981
                     /locus_tag="VXN30_000368"
     CDS             18574..18981
                     /locus_tag="VXN30_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HEV1357042.1"
                     /translation="MRENDFQNVLLKHIKTLNLPVEPRFDYFEDDKDDLVINQIPGGK
                     VDREYMDGTQEVSLPFEIAVKAKKNSVANDTIWLVTSELAKIDLVLPSDNNSYEYMGM
                     EVSRPAMKGKDEQGYYYYTIEIVAKIVIERNKQ"
     gene            18978..19427
                     /locus_tag="VXN30_000369"
     CDS             18978..19427
                     /locus_tag="VXN30_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1357043.1"
                     /translation="MTRQKNALRGHFVAPYNGGTEPTTEDTWLELAKWISDVSDDTDE
                     KTDDQAYYDGDGVEETTVVSVKGAYTFEGTYDPDDKAQALIAGMKYKTGDDRKLWHKV
                     VSSDKKKQWVGAATATEIKAGSGAASDYEAFGCKLSYNSTPKETGIG"
     gene            19492..19980
                     /locus_tag="VXN30_000370"
     CDS             19492..19980
                     /locus_tag="VXN30_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357044.1"
                     /translation="MTDIQIELKRTGFPVKIGEVELWFDTSQESLMRFYDMEEELKRR
                     LVQYELDVVSANINNKIERDGVTKEVVAGAIELEKKQLEIQYDLIFGDGTFDKLYSIY
                     PDYNALNNALEQTAIMLHDKLEELAEQHKTVVKERASHYLNKGKVTPIKNNKKQKKNK
                     KK"
     gene            19993..20562
                     /locus_tag="VXN30_000371"
     CDS             19993..20562
                     /locus_tag="VXN30_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357045.1"
                     /translation="MSMKLNDALITNFSIADKEYDIDLSFNKVLDVFEILKEDEMTRL
                     EQAQLIVHLLTGQELYDIKEVVDCWIYIKEHFLGIEKETVQYDLLGNPMPKAKGEEEQ
                     EKLIDFEQDAEYIYASFLQAYGINLLKVQNELTWTEFKALLNALPDNTIMQQIIEIRA
                     WKPEYGGDKNKMRKLQAKYSLGKEGEDNG"
     gene            20555..23836
                     /locus_tag="VXN30_000372"
     CDS             20555..23836
                     /locus_tag="VXN30_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEV1357046.1"
                     /translation="MADGKVTIVVDVDGNKVKVLNDELDKAAQKGDRGSDSLKKFAIG
                     GAAFKLASKAVDLLTDSLGGAIQRFDTLESYPRVMQAMGHSTEDVTRSTKKLAAGIEG
                     LPTTLNEVVGTAQRLTSITGDINKSTDLTLALNNAFLASGSSSADASRGLQQFSQMLS
                     AGKVDMQSWKTLQETMPYALQKTADSFGFAGQSAQNDFYSALKEGRITFNQFSKKLVE
                     LNGGVGGFAELAKSNSKGIQTSFGNLKNAVVKGVANTIKALDDLTKAATGKTIAENFD
                     ALKVIINAAFGVIVNVIKASTPVFQTLFSILGTGASVISSLTPVIISLVSALVAMRAA
                     NEAITATKNLINSWQTFKTTATGAIQIINLMTAAQATCGSVTKAQLVANLANNGALTA
                     SNLLYGVLTGSISLQTAATIAATAATTAFKAALTALTGPIGLVVAGVGLAVGALVGLW
                     QWLTAESEETKRLKSEQEELVKSTDQLTDSVKQSAKERQKNLESVKGNTESYQKLADE
                     IVQLSQKTNKTAADKKNLKKKIDALNASVSGLNLVYDKNTDSLSHNNDQIKARISAME
                     AESTWETSQKNLLDIEQKRAEIGEQLKQIAEQRKKWNEESNVSDSVRKERLQELNDKE
                     TELKNTQTELQTEYEKTSQVQQAASEAMAAAAENGSNRQVISYEGMSKAQQKAVDDMR
                     SKYNELLETTTNMFDQIQMKSAISVDEMIANLQKNQEAVNNWATNLNTLAERGVNEGI
                     LAKLQAMGPQGGLYVQELVNASDEKLATLNEVFTQGGESAMNGLTAGMDTGALGITDK
                     IKGIVQSQVSSLQEEIAAADFPEKGKNIPEGVGDGIKAGAEIASEASKNMANDIKESF
                     TSEMDINSPSRVFNEYGGFITTGLAEGVDKGTNQPVSSVTNLANQIKKPFDSLQSDFT
                     YIGEMAMSGLNAGLWSGSGSVMATANSIAERVKATIKSALDIHSPSRAMRDEVGRFIP
                     QGIAVGIEADAGVVEKSMLRLKESMMIDTRPEIALGLNKKLGAQVTVKQSSKQTIAEK
                     IKVTMDKSSELLEKALDVAETAVRRPNEMYLNDGTLVARTGDKFAKYQSEQLRRDNRM
                     KGVLS"
     gene            23833..25347
                     /locus_tag="VXN30_000373"
     CDS             23833..25347
                     /locus_tag="VXN30_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015968624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEV1357047.1"
                     /translation="MTKIMTFNGVDMSKFFRITDIIRPIGNKRSVSTDNAPLLGVNIQ
                     QVKIGEKEHIIKFDIKTTNAIEMEQLKHDLAGILNVLEPVKITYGDEPDKYYMGLPVD
                     EITPENLTRWFQRSELKIIIPDGVAHSTTLKNFDIDTNETSAPDRIVFNLTNTGTEPA
                     YPIIRIKHNSENGYIGVVNNRAAFELGNREEVDTEKYRDSETLIDYRGTNILKGFQNG
                     TKGVAVTNDNKERLVGTLSTTSMWGRNHIELSNRGTVEKNRNNAQSLTWAIPVDSSGE
                     VGSLNDYLLWRQVFMAAVANQYGFIKVTVSDTDGNFLYGVETYKRYQTLDCEYSFFTT
                     DGKGGYKFIKWWYFTGTGAQVGKLDPFSAEKGWSELKRNDDRVQVFFDGSHYDFIIPE
                     IKDKKSAKIHITLGALRDWPLVSHMYVDEFMYRKDFVTKSRDIPNRYPIGSNVVINSE
                     DDSVYIDGISKVSEVVDGSHWPVIPPGKSQLELYFSRFVKKKPTVTIEFEERWL"
     gene            25349..>28038
                     /locus_tag="VXN30_000374"
     CDS             25349..>28038
                     /locus_tag="VXN30_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1357048.1"
                     /translation="MLLTIHDANLQKVAFIDNEKQGTLNYYDDTWTRSLPTGSSTFEF
                     TVFKKAVKSDLPLAKAYHHLNEHAFVSFKYKGKSFVFNIMIVEENEQTIKCYCENLNL
                     ELINELANPYKSNKAMTFKEYCEAMDLLNYTHLSIGINEISDYKRTLEWEGQETKLAR
                     LLSLAKRFDAEIEFDTQLNADSTIKKFSVNVYHENDDNHQGVGRVRNDVIVKYGKNIH
                     SITRKVDKTGIFNTIRPTGKMPTVEEEPSGDKGSKSETVKNADGSTTKTTISTASDGT
                     KSKTIVHTKVTKLADKTRITTTTTTRSDGSIEQTVTTSKKGGASTSETKVLKKPNPKE
                     KTNTTEDVLTIEGLDEWEVKNEKGIVEFYQRGQALYAPISMQLYPSTFTHSTGELDQW
                     TRKDFHFETDEPNELRRLGYLKLKKYCYPAITYEVDGFVDADIGDTVKVHDDGFAPLL
                     MIQARVTDQKISFTNPVRNKTIFDNFKALENKLSADIQSAFERLFEAAKPYTIKLSTD
                     NGVIFKNQIGQSLVTPTLYKGGKPVVVGVTWRWALDGEVTTGMTYLVRGSNVTDTVTL
                     TVAAYIGNKEVAVDEISLVNVADGKLGTPGTPGRDGRTPYVHTAWANNATGTDGFSLD
                     SSINKLYIGIYTDFEPNDSTDPKKYKWAKVKGDKGEKGDKGEPGQRGLDGLQGARGEQ
                     GLPGRNGADGRTQYTHIAYSNSADGTKDFSVSASDRAYIGMYVDFNSADSNTPSDYNW
                     TLVKGADGANGVAGKAGTDGRTPYLHIAYATSNNGSQGFSTTDSTNKTYIGTYTDYTQ
                     ADSTDYRVYKWTLIKGADGTGISNVTNYYLATTVSTGITRTSAGWTTTPQPITSDKRY
                     LWNYRVELYTNGTSKTTEPTVIGVHGEKGERGLQGLQGLQG"
BASE COUNT         9815 a         4583 c         6203 g         7437 t
ORIGIN      
        1 aaagtatctg agaaactcag acactttcta aaaaaatcta acttaattat agcatgaaag
       61 gggaaaaatg gaaacagttc aaatcgtgag aattaaagat gtgatcatcg agaagatttc
      121 tgcaaacgat gaagaactag agcgcatctt tggatgttca aaacggcaag cgggagatat
      181 gaggcgagag atgaaaaaat tgcctagtca gcaaaaatat cttagaaacg atggtcagct
      241 tgtcacaatc aaaggttttg atgcttatct gcaatatcga ggcagtcaac catggaagaa
      301 agaaatggct aaaaccgtta agatgacacg atagcagaat aataactact aacaaataac
      361 aaactcatcc ttatagaaat aagggattta tagagttttt aaaggaggag ggaaatggca
      421 ttatattcag aaacagaagg aacaaacatt gcagaaaata ttaaactaat aagattatca
      481 ttaggggaaa ctttagagca gtttgcgaaa cgatttggaa catcaaaagc agtggtttgt
      541 aattgggaac acaagcgaaa tgcacctaaa ttacctacca tgttgaaaat tgctgatttg
      601 gctggtgttt ccgtaattga ttttctcaat aaaaaaatcg aggtgtaaga ggtgtaagaa
      661 tggaaaattt agataatcaa gaaattacaa ttactagagg tgagctgaag caacttattt
      721 cagatgctat tcgtgaaaac ggtaaggtga caagtaatac tatctttaat aaaagcacat
      781 caatcttaaa gcgtaaaagc gagttacatc tgaaataccc gatcctaaga aagatggtgg
      841 aattgtcagc atcaatatat cacccagacc cgtacagatt tgagttcgcc tcaatcaatc
      901 gctttaatca agcacctagt ttcaaaagaa ttgggagtgc taatcaatta catgaaatag
      961 caagaaaatt tgccttgtta tgttttggag ttgatactaa tactgacctc tcagcatccg
     1021 atgaagaaaa agctgtatct atttacaacg aagtaattga gatatttttt aacaactatg
     1081 aaaaacacct tgaggataaa tttggaggtg acaaatggca gaaacactaa gaaaaaagct
     1141 attgaatatc cagcgtgact tgaaagcccc taaagggcaa tacaacgact ttggcaagta
     1201 cagctatcgt aacgcagagg atattatgga ggctctgaaa ccattgctag ttggtaataa
     1261 tacagtttgc tactgtaagg atgattacat cgaacaaatc ggagaacgtt attaccttgt
     1321 ggccactatt gtttttgctg atgttgaaac tgatgaggaa atcacagtaa aggcaagggc
     1381 gagagaggaa gagaacaaga aaggcatgga tggttcacaa atcactggtg cagcatcaag
     1441 ttatgctaga aaatatgctt taagtggtct tttcttgatt gatgataata aagatgctga
     1501 tagcaatgag tatcataagc aaaacgatca gcagaaactc aaacaacaag gtcaacagca
     1561 acaacaagga aatcagcaag cccaaggaca aaatcaagta aggtatattg acaacactca
     1621 atatcaagaa attaacgacc ttataaatga tattgcaaaa attaaaggga tgccgttcga
     1681 tacgcttgct aactatgtac tatctgaaaa attaaaaggt ttacaagatt ttcatagagt
     1741 acaagttggt gactacgagg tattgaaaaa ctatttaact gaacaactag caaaggcaaa
     1801 ggcaaaagca aagagaggta attaaacatg gtaaaagatg taactaatag cttgacagaa
     1861 atcaaggtgt attttcaacc tgcagtaatt aatgttgacc gtgaggcgat cgaggcacaa
     1921 gtagccacag tcattgcaca gtatagcggt cgcgaggtta ctgttgataa ttacaaagaa
     1981 gtttatgaag agcgaacccg ctttaataag ctgattgggg gcttagacac tcaacgcaaa
     2041 gattttaacc gacaaatcaa tgagccggca aaagactttg ataagtgggt caaagaaaaa
     2101 gtcatcaagc ctattgaggc agtgacagat gctatgtcag caggacttaa tgcgattgat
     2161 gaacatgaac gattgatgcg cgtggatgtc gtgcgtgcta catttgagga taagtgtatg
     2221 gtcgcaggga ttgaaaaatc cacattcgct gacaaatacg atgagtacag cctcaagaaa
     2281 tattttaaaa caggcaagta tgagctgaaa aagacaacac ttgatgaaat ggatggctta
     2341 gtactttcag aatttgatgc cctggaagaa tacaaggcta acaagcaagc tatccaagag
     2401 caagctcaag agtacgattt gccagctgat agctatatca gacatcttga agatggtaag
     2461 agtcttgttg atattctcaa gatgatgaaa actgatcgag atgctgagat tgcacgcaag
     2521 gagcagaaag aaatccaaga aaaagcaaaa gctgaacgac ttgaagaaat tgctcaatcg
     2581 gccaagaaaa atgctaatgc gaatatcaag gcttacgatg ccgaaacagg cgagattttg
     2641 gaacagggta caattacacc agaacctcaa aacaatgagc gagaggtggc aaaatttgag
     2701 cctagcgagc ctttggtcaa attagtacgt cttgaattgc acggtggttt agaacagtgg
     2761 gaaaatacac aagaatattt tgaggataac tttatcggtt ttgaaacttt ggaggattaa
     2821 gtagaataag gagtcagacc tatgagatgt ttttatgtca gcggtaaaat tgcagatctt
     2881 gatttggggt cagaaatcaa tgcagaaaat tcatttatgg ctgctattga gtttgtgaaa
     2941 cgatataccg acttattaaa gtttggttca aatgaaatca aggtatcaga agtagaggag
     3001 gtgcaaaatg ataaataacg ttgttttagt agggcgactt acaagagatg ccgaactgag
     3061 atacacgcaa tctaatattg cggttgctac gtttactctt gctgtaaacc gtccatttaa
     3121 gaacgaggct ggagagcgtg aggctgattt tatcaattgc gttatctgga gacagttagc
     3181 tgaaaatctt gctaattggg ctaaaaaagg ctctcttatc ggagttacag gagtaattca
     3241 aacacgtagc tatgataatc aacaaggcca acgtgtttat gtcacagagg ttgttgctag
     3301 taatttccaa ttgctagaaa gtcgtaacag tcagcaaaat aatcaagata attatggcgg
     3361 ttatcagcaa cagggttaca gcaaccaggg cagttctttc caaaacggaa ataacacagg
     3421 gaacaatttc caaaatggaa atagttacgg acaacaaggt agtttctttg aggggaacac
     3481 aacaaatcca gttcctgatt tcacccgtga taacaatcca tttggcagac cgacaaaccc
     3541 attggatatc agtgatgatg atttgccttt ctaggaggtt ctaagtatgg ttggatatag
     3601 atgggaacta cctttagcat cagcagaacg caacaacaca ggatatatac atagcaatgc
     3661 gaaacctcat ctattcaata ttgtaacagg gtggtcacat tgcaagaggt attggcaagt
     3721 acctctttgg gctgaggaaa taaaatttga tggaaaggaa aagtattttt gtaaacggtg
     3781 tttgaaaaaa tacaagaaat tgcaaggagt agccaatgaa caaagtaaaa gttgatttgc
     3841 aatgtccgtt ttgcggattt tgtaaagttc taaagatacc aagccaccga aaaggcatca
     3901 catgccctag ttgtcaacaa tcagtatttt taagttggtc aactggtgta gagggttatg
     3961 ttgatgagca tggattttat ttccacgctt acgagccgtt caatatccga aaaatcaacc
     4021 gagagtttca agatgccttt gaggatgcac cacctcgacg acctttcaca atcagaagta
     4081 agacgaaaga gtgatatggt gcagaaaatg aaattcttag acttatttgc tggcattgga
     4141 ggttttagga tcggcatgga atcagccggg catgaatgta taggattttg tgaaatagac
     4201 aaattcgcta gagctagtta taaagctata cacgatacga aaggagaaat tgaattacat
     4261 gacattacaa cagtatcaga tgacactatt cgaggaatcg gacatgttga tgttatctgt
     4321 ggaggatttc cgtgccaggc tttcagcatt gcaggaaaca gacgaggatt tgaagataca
     4381 agaggaactc tcttctttga aatcgcaagg ttcgcatcta ttctcagacc taaatatcta
     4441 ttccttgaga atgttaaagg attgctcaat cacgaaaatg gagttacatt cgagaccatt
     4501 atctcaacct tggatgaact ggggtacgac gtggaatggc aagtgcttaa cagcaaagat
     4561 tttggagtcc cccaaaatcg ggaacgtgtg ttcattatcg gacatcttag aggaggaagt
     4621 ggacgaaagg tatttcctct cagcagagaa aatcagtcaa ttagtaacca atcagtcgtg
     4681 aaaattggta atgtgaaccc ctctggaaat ggcatgaatg gggaagtcta tcaagctgac
     4741 ggcctagctc ctacgctcac aacgaataag ggagaggggc aaaaaatagc tataaaaagt
     4801 aatactataa aacaatttgg ggtattgcaa cccaatttca atcaatgtgg ggtagtttac
     4861 gaaacagacg gtatcgcacc aactatcagg gcatatcaag gcggaggact tgagcctaaa
     4921 attattcaac gtggtcatgg ttataataaa ggcggagagc atgacatcgc tcctacttta
     4981 actaggaata gctatcacga aaacaatgtt ttaaaaataa cagaggcaac ctctcaaggc
     5041 tacgcagaag cgacgattgg agatagtgta aacctatcgc atccaaactc taaaacacgc
     5101 agagggcgag taggtaagaa gatagcaaat actttattga ctggcgaaag ccaaggagta
     5161 gttgagcctg atttcaggat tagaaagcta acacctcgtg agtgctggag attgcaagga
     5221 ttcccagact gggcttttga taaggcgcag gaggtcaact ctaacagtca attatataag
     5281 caagcaggca atagcgtgac tgtgagtgtt attgctgcaa tagcacaggg gttaggagga
     5341 aattaaccct atttaaacca atttgaaaag gaaatagaaa atgacaaaaa ttgaaatcgt
     5401 tatggtactt acaactttga tgtctatcac atgggcagcg attgttacaa ttcacactat
     5461 gcaagctatc aaaaagcata aagcaaaagt ggattattat cagaaaccac aagtgcaatg
     5521 cgagattgca cgtcatgtac taaaaaacag atggtactca gatggtgggg aggtgtttag
     5581 atgaaagtat ttgatggagc tatttcaaaa tcgtacattt agcgaggttg agcaaccggc
     5641 aagcctattt gtctttagga ttgataagca agccaatatg gctttatttg aggcagatgg
     5701 taagcgttgg gtagctgatg cagtaggaaa tgttgcagca tatctaaaag agcaattagc
     5761 agaccaaaaa catatcacag tattagcata agaaagagga aattaaaatg agtgattaca
     5821 aacaacggat gattgaagaa tacaaacaat taaaagagcg caccaataag ttaagtttga
     5881 tgattagtaa ctattacgta ggaacacttg attttaaact aaaatgtcct attgagttac
     5941 ttgaaactca acactataca atgtgtgcat atctcaagat ccttgaacag cgtgcagaaa
     6001 ttgaaaacat tgagttttaa ggtaatcaaa atgaaatttg agttttcttt gcctcggaat
     6061 actaagctaa aatctctaaa catggttatc aatagtaatg acagacaaca tcaaacagat
     6121 aaggctaaag ttactaaacg cattagagct tttgcttatt ggcatacatc gatgaacaag
     6181 gataaaggga gggctgcttt tagcccctcc aacccttgtg aggttacagt tacaatttac
     6241 agccctacta agtcaaaact tgacccacct aatttatatc caacagtcaa ggctatcatt
     6301 gatggcatga ctgatgcagg tatttggaca gatgataatc ataaggttat caaaaagtta
     6361 tcctttgttt atggtggatt aagcgaggaa aaagggcatt atagattaga gtttgatata
     6421 gaggaggttt aaagtgattg aagttaatat aaaattcgat aattttgaag cgcatggctt
     6481 ttaccaagat gatactaaac taggaaaaat tagagatgca atcatatctc aaatgaataa
     6541 tgggcatgtg gttattttag gggaagatag aagtattcta ttaaatccta aagttattaa
     6601 atgtgtacaa tttgaggttg tagaagatga ccagatttaa tcgttttttg acctttagag
     6661 aatctgagtt attttgagga ggtggagtga tggaagagtt aaagaaaaaa gttaatgaag
     6721 tatacaactg gacggtagaa gacgggaagc cgcaacctcc caagcaagat ttaccacaag
     6781 tggtgaaaga ccgggcggac tatttttggg aaatggcaga agatggtatg acgtttatgg
     6841 gagcgatgga atgcatcttc gctgatgaaa agcctacaga ctatgatttg ggagctacta
     6901 aggattggtt gccaaaatct aaggagtttg atgattgggt tggctattcg ccaggaatgt
     6961 ctcaggtagt tattgcagtt tatttgattt atagaggaaa ctaagatgaa tattaaggca
     7021 ttgattaaga agtatgaaga attgtggaat gaacacagcc ctttttatga acctgtacct
     7081 tatacttcaa tggttgaact ttttttgaaa gagttgaaac aactagacga accccaaaaa
     7141 gtcaaaattc cgcagtttgt ggcggaatat atagaattta aaaagaaaaa caattttcat
     7201 gtttacggtg caatgagagt aattgaagat cattatgata agaaagttcc tgattggttt
     7261 tacgaaaata acatcgaaaa attctgtctt gcttggctta acggctatga ggtcgaaaaa
     7321 gagaagcggt atttggtaaa gattaaaggg aatattaaag aaaatatgtt ggtttatgga
     7381 gaacttttgg aaaggtactt ctttacaaaa agctttagtt tagacgatgc tatatattcc
     7441 cacacccgta aagaactaga aaatgcaaaa atcggctggg tgtttgattg tgaagggttt
     7501 gagattgagg aggtggaata aatgacaaaa tttgttaaaa tacaatcttg ttatagagga
     7561 catactgaag atgagcttat caacatagat gatattgggc gcatctgtct aggccctaat
     7621 atcttgtttt taagaacacc ttacaattta ggagaacatc atatttctat cactcaaaat
     7681 tcagtagata aacttttgaa agtattggat attattgggg aggacggcaa atgagacctt
     7741 gtaaatatcc atttttggga agaagaaaaa aacaagaaac atcgtcgcca ttattttctg
     7801 cacgaccaat tttaaacgaa gttccaattg tagaagaggt caaagttgat ctcggagttg
     7861 aagctaatgt tggacgttca tatccagaaa tggtaataca tttagatatt tctggatacg
     7921 ggaatagagt gcattcggta catcgtttcc ctggtgtttt tcttactgtt ggtgaatcaa
     7981 tccaactaaa gatgcttttc tataaaagac ttagaaattt taccacagat cgttttttga
     8041 cctttagaga atctgattgg aatctcttta tcagcgatct ggtcaacgaa tttgtgcgtt
     8101 agaaagttaa tgaggtgaag taatgacaca aacacttgaa caagctgcaa aagctgaaag
     8161 caaacgcata aaaatccctg cgaaaatcag accgttcgat gtgggttatc gaataataaa
     8221 taaacacggt caagcgcttg ccttaaaaaa cggagcaagt atattcagtt tgccatcatt
     8281 ggctgaaaaa gctatagaaa aagagtttgg aaaaaatgat ccaaactttg atataggaaa
     8341 gcattctgtt gaagaggttg ctattgtcaa tttaagtaaa tttcatagtt actttgagga
     8401 ggtgatacat gactgatgac gaagaaaaaa atagagcgct tgtcagttat ccatcgcagg
     8461 gaaatcaatt ggctaaagtg gtattttttg agggataaga aaaatccaaa gagaaccatt
     8521 ttggagcaaa agattatagt ttctcatatc aaaactgata ggcttgaagc taagttttta
     8581 agcaacttaa aaaaatcaac tgaagatttt atagataagt ctgatcctaa atatttgcgg
     8641 gcaataaaag aggtttatgt ttacgaaaac atgaatgtca ttggagcttg tcaaaaaata
     8701 ctattttata gtccgactca agcctatgta ttacttaatg cgtggtttaa cgattatttt
     8761 cgtgcgactt acacagaatt actagaaaac gccatcttag ataaataacc gtaaaaaatc
     8821 caaagcttat gtatctataa tcaagatata taagcttttt cgaaaggaga gatatggaga
     8881 tgttacagat tgaatatgta gatataaaat ccattaaacc atatcacaaa aacgctaggc
     8941 ataatgacgg agaggcaaca gagaaagttg ctgcatccat aaaagctttt ggttttcagc
     9001 aacctatctt agtagatgat aataacatca ttattacagg acatactagg ctaaaggctg
     9061 ctctttcttt aggtatagac acaataccta tcgctcacgc tgtaaacctc acagatgagc
     9121 agataaaagc ttatagacta gcagataatc gagttgctga gtattcaacg tgggactcag
     9181 aattattaaa tattgagctt tctcaatttg aaacaataga tatggctcag tttggttttg
     9241 agttatcagt tacagggttc aactttggta acgaggagga gcagcaagag gaaactgaaa
     9301 acgaggagga agatgctgag gattttcata gagataccac aataaaccaa tacaaccttt
     9361 ttaattatga cgatacaagg gttgagggtt tttataacat gcctaaaatc gagggcgtgg
     9421 atcatattcc taaagatttt caaggcttta attatgtttt aaataaacca gattacagct
     9481 catgcgtgca ttttttccta gatgattatc aatttgaaag aatatggcaa agaccagact
     9541 tttacattga aaagctgcta gaatttgata gcgccttagc tccggatttt agcttatatc
     9601 ttgatatgcc tatcgctatg caagtatgga acatttacag gtcaaggctg ataggtcaga
     9661 ttatgcaaga ttacggcctt acagttatcc ctactgtatc gtgggctagt gaggaaagct
     9721 ttgatttttg ttttgacggc ttgcctaaaa actcaacgct agcaatcagt acaataggcg
     9781 taaagcaaaa caaagagcag tttgaggtat ggaaaaatgg agttactgag atgataaaac
     9841 ggttgactcc aaaaagaatt gtagtatatg gcggaaaagt ggaatacgat tataaagata
     9901 tagaggttgt atattttgaa aatgcaacaa cggaaaggat gaaagaaagt ggcacaaaaa
     9961 ctaactaaac taaaagatat ttttaaacat gtttcaagta ttgatctagg taaagagatt
    10021 ttatttgaag atcttgagct ttacaataaa gaaacagaaa caagcaaaca ataccaatct
    10081 atcgaggagg cagaaaatga cctatctttg atggaaaaag taaataaaat caatttcact
    10141 ctaggcggtg gacgtggtgc aaattttgag aaagggaaag acggtaagta tccgggtttt
    10201 agaggtgctg gtggtgcaag agatagtggg agctcaaaat ccttacatcc agcatcttta
    10261 aacaatcaag ggcgcttttc aagtgttgag ggaactatcc aggaatttat taaaaaacac
    10321 ggtggctcta gaacagaata cagtacagca gttgactctc aaggctttgc tcataattat
    10381 gtacacggtg ggaaaaacag cgtacaaatt ttgcctatct ctggtggatt tacagcaata
    10441 cataaccatc cgaatggcag caatttctca agtacagatt tacatagctt tgcagcatta
    10501 aaaggtatga atacactagt tgcaacgaat agctctaaag cgtatcgaat tacaaaaggg
    10561 gctaactttg atgctaaagg ctttgataaa gctgtgagca agtcacgttt tactacaaaa
    10621 gattacaata aaggagctga cctatggctc aagaaaaacg ctaagaaata cgggtacact
    10681 tactcatacg agtaaaagaa aagaggctaa ggtatgggtg gtagaggagc aaaattaaac
    10741 ttgtcaacaa cgaaaatgac aactcaaaat atagagactt gattaacaaa atcgaaaagt
    10801 tattaggaat agcgtaaaac atcccctttt ttaacatata caatgaaatc ataagtataa
    10861 aatgcttgtg atttttttgt ttgaaaggag ggtggaaatt gcctagagat ggaactaaaa
    10921 atttaaaacc agttacagaa cgaaccaaag atgaagcaag agctattagc tcaaaaggag
    10981 gtaaagcatc tggcatagca agaagaaaaa aagctgatct aaaaaaagca tttgaaatcc
    11041 tcttatcttt ggatgtgacg gatagtaaaa tcaagaaaca acttgaggag atgggtatgg
    11101 ctggcaataa cgaggctttg ctagcctttg caacctttca gcaagctgta aaaggcaatc
    11161 agaaagcgac tgagaacata atcaagctga caaatactaa agataaatac gatatacaag
    11221 aacagaaaga gcgtattaaa gcactcaaat atgaaaatag agagcgtgct gaagctgaga
    11281 aaggctcaag tgaaactatc gagatagtgg atgcatgggc tgaagatgtg aggggggcaa
    11341 cggatgacct ttaatgtcca gaagaacatc aaccctcatt tcaaatctgt ttggatttct
    11401 agcttacctt ataatgtttt gaaaggtggg cgtaactctt ttaaatcatc ggttattgta
    11461 cttaaactag cgtatatgat gataaggtat attatcgctg gagaggcggc caatatcgtt
    11521 gttatccgta aggtggccaa tactattaga gacagtgttt tcaataaagt ttggtgggca
    11581 ttgaaccttt ttggtatagc tgagcagttc acaaaaacag ttagcccgtt taaaatcgta
    11641 cacaaaacga ccggctcaac attttacttt tacggacaag atgactttca aaaactcaaa
    11701 tcaaacgaca ttggaaatat catagcggtt tggtatgaag aggctgctga atttaatgac
    11761 caagaggact ttgaccaatc aaacgtgacg ttcatgaggc agaaacaccc acgcgccaag
    11821 tttgtacaat tcttttggag ttacaatcca cctagaaatc catatagttg gatcaatgaa
    11881 tggtttgaga gcatcaaaac gaataagaac tatttagctc actcaagcac ctatcttgat
    11941 gatgaactcg gattcgttac tgagcagatg ctagaagata tagagcgtat caaagagaat
    12001 gactacgatt attacagata cttatatcta ggtgaggcag tgggattagg taacaacgtg
    12061 tataacatga gtatgtttca tgctattgat gctttgccta gcgatgataa gctgattggc
    12121 atatcatttg cgctagatgg cggacatcaa cagtcagcaa cagcttgttg tgcttttggg
    12181 ataacagcta aaggaaaagt aatcttactt gatacctggt actactcacc tgctggccaa
    12241 gtggtcaaga aagcacctag tcagctatcc aaagagatat atacttatat acgctcagtt
    12301 attgagaagt acagagtaca agccttgcaa tacacaatag atagtgctga gggagcattg
    12361 agaaaccaga tgtttcttga ctttggtttg aaatggcatc cagtcgctaa acttagaaaa
    12421 gtgactatga ttgacagttt tcaatctttg cttgctcaag gtcgctttta ctatctcaac
    12481 accgaaaaca acaagatatt tattgaagaa cacaagatgt atcgttggga tgaaaagact
    12541 atcaaatctg ataatcctag cgttatcaaa gaagatgacc atacatgcga cacaacacag
    12601 tattttgtgt tagacaatgc aaaattgctc ggtttgcgtg ttggtaacgt ttagaggagg
    12661 acgatcatga gcctatttca aaaagtaaaa gactttttta gtcgagggag gtattacatg
    12721 cagacatcaa atcttaatag tattttggaa catccaaaaa ttgcagtgac tcaagaggag
    12781 tatgaccgga ttaagagaaa tctagtctac tatcaatcaa aatgggatga tgttcagtac
    12841 aagaatacgg atggagatat taaatcccgt ccaatgaatc acttgccaat tgcaagaaca
    12901 gcatcgaaga agattgctag cttagtttac aatgaacagg caactatcac aacaaaaaac
    12961 gaaattttac agaaattttt ggatgacatg ctaactaacg accgattcaa taagaatttt
    13021 gagcggtatc tagaaagctg tttggcactt ggtggcctag ctatgcgccc ttatattgac
    13081 ggggataagg tcagagtggc atttattcaa gcgcctgtgt tctttccact agaaagcaac
    13141 acacaagatg tttcaagtgc tgcaatcctt actaagacta tcaaatctga ggggcgtaag
    13201 aacgtttact atacccttgt tgaatttcac gaatgggtaa cagcagacgg acaagaaaca
    13261 ggtagcacaa acgataaaaa gtattatcgt attacaaatg aactttatag gtcagatgtg
    13321 aatgatgtgt taggtcaacg tgtgaacttg agtgaactag acaagtacaa aaatttagag
    13381 cctgtaacag tctttgaaaa cctatcaaga cctctattta cttatctaaa aactccaggt
    13441 atgaataaca aagacatcaa cagccctctt ggattgtcta tctttgataa cgcaaagacg
    13501 actattgact tcatcaatcg ttcttacgat gaatttatgt gggaagtgag gatgggacaa
    13561 agacgagtta ttgtgcccga acatctaaca caaagacaat atcaacgtcc agatggaaca
    13621 atagatttta gaccacggtt tgatgttgag cagaatgttt atatgcaaat tggcggctct
    13681 agcatggatg ccgggggcat tacagacctt acctcaccaa ttcgagcaaa tgattatatt
    13741 ttggcgattt cagagggatt gaaactcttt gaaatgcaga ttggtgtatc aagtggcatg
    13801 tttacattcg atggtcaagg aatgaaaacc gcaacagaaa ttgtcagcga gaactcagac
    13861 acttatcaga tgcgaagtag tattgtcgca cttgttgaac aatctatcaa ggagctttgt
    13921 gtttcaatgt gtgagctcgg taaggcggta ggggtttaca gcggagaaat tccagaactt
    13981 gatgatattt cagttaattt ggatgatggt gtatttactg ataggcatgc agaacttgat
    14041 tattgggcta aaatggtagc cgcaggattc tcaaccaaaa aacgggcaat tggtaagaca
    14101 ttaaatattt ctgatgttga ggcagaaaaa gaactcaatg ctatcaatag tgagttgcca
    14161 cctatgaatg atgccgaact tgctatttat ggtatgcatg accaaaacga ggaggaagca
    14221 gatgacaaag gttaaattcg gagttactag tgttgactac tcagcaagca ttgaagatac
    14281 gccaacaata aaactaggtt taataattag agggagcggg agactagatg cctcttcagt
    14341 tattaaaaaa ctaatcaagg atgtttctga actagaatac gaattagaag aataaactgg
    14401 tcaattggcc aattttcttt caagagaggg cttttgaatg aaaaaaaaga gaaaacagat
    14461 cacgtttaac gaccaacaat ttcctttgca aatgcaaggc gttggggata tttacgaaaa
    14521 attacagatt gatatctttg accgtatgat aaaacgctta aaagagcgtg ggtctattga
    14581 tttaatgaga aacccttata tctggcagtt agagaaacta aatgatatgc acatgctcaa
    14641 tgaacagaat ctaaagctta tttcagagcg tacaggaatt gctgaaagat tgttgcgtga
    14701 tgtaattgag aatgaaggtt tgaaagtcta taaggacact aaacagcaac ttgaagaaga
    14761 tttgaataaa atacctgagg gagagatttc aaatggcgta acggacagct tagaggctta
    14821 ttcgaggcaa gcagttagtg atttaaacct tatcaataca acattgccta agagcttgca
    14881 agtagcttat aaatcgattg tggaggagac agtcgcacag gtagttgcag gaactaaaac
    14941 aagcgatgtt gctttgcatg ataccatcat gaaatggcag aagaacgctt ttacgggctt
    15001 tgtcgataaa ggtgggaggc attggaaagc tgatagctat gcgagggcta ttatcaagag
    15061 cacaacatac aaagtttata acgaaatgcg tactagacct gctgaggagt taggagtaga
    15121 tactttttac tattcgatga aagcaatggc tagaccagct tgtagtccgt tacaagggca
    15181 gatagttaca aaaggggctg gtagggagat agatgggata actatctatt cattattgga
    15241 ttatgggtat ggaacagcag caggatgttt aggaatccat tgtggtcatt atctgacacc
    15301 gtttattgtt ggagttcatg agttaccgaa cttaccagac tatctgaaga atctaacacc
    15361 agaacaagct gaagaaaatg cacgcattga agcaggtcaa agaggccttg agagacttat
    15421 caagacacat aaagagcggt tgcattacgc tcataccttg caagatgaca agatgataca
    15481 agctgagcgt ttgaaagtta gagggtatca aactaagatc cgcaacttga taaatcagca
    15541 tgatttctta acaagagatt acagacgaga gaaattatat atttcataaa ggatttgtgt
    15601 ttcacaagtc cttttttgtg tttaaaaccg taaaaaatcc ctatccatca aaggtatatt
    15661 gagagagtaa ataatatttt gctttaggtg ggagttatcc acctaaaaaa gaactaggag
    15721 ggtacaaatg gcatttacaa ctgaagaact actcaatctt gggttgacag aagaacaggc
    15781 taagtcagtc tttgctttgc gaggaaaaga gctgaatgag gacaaatcag ccttagaaac
    15841 tatcaaacaa gagcgagata gtctcaaatc acagttgcaa aaagcagagg agcaagttga
    15901 acacttgaaa tcacttgaga atatcagcgc tgaacaaaaa gatgcgattg ataaattgca
    15961 agctgaatat gacaagtata aaaacgaagc tgcagctgaa cttgcacaga caaaaaaggt
    16021 tagtgctatc agtctagctc tgaaagatac aaatgctttc aatccagaca aattgatgaa
    16081 attcattgat gttgatgcta tccagttaga cgacaacggg aaacctcaga ttgatgaagt
    16141 aatcaacggt ttaaaagaaa gtgatccata tctgttcaaa gctgaagaaa gtaagcctag
    16201 ccccaatatt ttacctcaag gtaatccggc aggagagggt acaagtgatg ttgatccgtt
    16261 ccaagcgatt attgacgggt atggcaaata acagaaagga gattacaaat gccaagtaat
    16321 caaaacaacg cagtgcgccg ctatgagaaa caatatgcgg gcattcttga gacagttttt
    16381 agagtgcggg cagcattttc aaacgctcta gcacctattc agattttgga tggggtacaa
    16441 gaaaactcta aggctttctc agttaaaaca aacaacacac cagttgtaat cggtgagtac
    16501 aaaaccggtg aaaatgatgg tggttttggt gataactcag gggctcagtc acgctttggt
    16561 ggtttgacag aagttaaata tgaaaataca gatgtcaact atgactatac ccttacaatc
    16621 catgaggggc ttgaccgtta cacagtaaac aatgatctta acgctgctgt tgccgaccgc
    16681 ttgaagttgc aatccgaggc acaaactcga acagtgaaca agcgaattgg taaatacttg
    16741 tctgacaacg ctactaagac ggaagccctt gctgatttta cagatgacaa agtaaaagct
    16801 ttattcaata agttgtcggc tttttacaca aacaacgaag ttacagcgcc gattactgtt
    16861 tacttgcgtt ctgaatttta caacgccatc gtagatatgg catcagttac aagcgccaaa
    16921 ggggcaacta tctcccttga tgaaaacggg ctaccaaaat ataagggctt taccttggaa
    16981 gaaacgccag cgcagtactt tgagacagga gttatcgcta tcttctcacc aaacggtatt
    17041 gtcattccgt ttgttggtat ctcaacagcc cgtgtaattg aggctgaaaa ctttgatggt
    17101 gtgaaattgc aagctgctgc taagggtggt acttacactc ttgatgacaa taagaaagca
    17161 atttgcaaag ttacaggaac tattgtatag gaggtagaac atggcacttt acaaagcaac
    17221 aaaaaatctt ttctttgagc aactcaacat ggatgtgatt gtcaatgaca ttattgaact
    17281 tgatgaagat tatgctaaag aagtcaacaa gaaactaaaa aatgcttttc cagatgtgaa
    17341 aaatgtttta gaacttgttg acaaaaatgg aacgcttgaa ccagaagatg ccccatctgt
    17401 agatgatgca tctcaggcaa ctgttgaaga ttaaataagg ggtggcaaca ccctttattt
    17461 ttaagggagg ttacacatga cttatttgac acaagaggag ttcgatgagt taggttttga
    17521 tgaagttaca gactttgaaa aattggcaaa acgggcaaag atagcgattg agctatacac
    17581 aaacggtatt tatcagaaag acattgattt tgaaaaagaa attgcctatc gcaaatctgc
    17641 tgtaaagctt gctatggcat tccaaatagc ctatctcgat gcctctggta ttatgtcagc
    17701 cgatgacaaa cagctagcca atagtgtctc tattggccgt acatcaatct cttatagcac
    17761 ctcacaaagc acattagcag gtcagcgatt taatttgtct atggatgctg aaaatgcttt
    17821 gagacaagcc ggctttagcc tagttgttgg agttgcctat gatcgataag cggttattaa
    17881 aagggattga caagcgtttg ttaaaggatg tcctaaccat aaaaaaagta gctgataaaa
    17941 acgattatgg ggatgaagta tattcagaac cgttgactat taaaaatgta cgttttgata
    18001 gatcagtggg gagatctggt aatcgtaact caaaaactgg tacaggaaat tcaaaatcaa
    18061 ggcaaaaaca aggggttata tacctctatc cctcgctatc ttttgtgaca gttgataaca
    18121 gttggatggg tgcaaaagta aacgatggga taggagatta tacaattaat ggatttcaaa
    18181 ctaactatta tgatggtgag atattcagtc aagaaattga ggtgatctaa tgagtatcgc
    18241 cattaaagtt gacttgcaga aagctaaaca gaaactttcg aacgaatcca tgacaagagg
    18301 aaagattgca gtcgctagca aaatcttgct agacaatgag caatatatcc ccttgagggg
    18361 tggagagttg agagcttctg gccgaatcgt tggacaaggt gatgctgttg tctatggaac
    18421 agtttatgct agagcgcaat tttacggggc aaacggcatt gtcaccttta ggagatatac
    18481 cactccaggt acaggaaaac gatgggatca agttgctact agtaaacatg ctgaagaatg
    18541 ggctagagct tttgtgaaag gaatgggact ttgatgcgag agaatgactt tcaaaatgta
    18601 cttttaaagc atatcaagac tttaaattta ccagttgaac cacgctttga ttactttgag
    18661 gatgacaaag atgatctggt tatcaatcag ataccaggcg ggaaagtgga tagagagtat
    18721 atggatggca cacaagaagt ttctttgccg tttgaaatcg ccgtaaaggc aaaaaagaac
    18781 tcagtagcca atgacactat ttggttagtc acctcagaac tagcaaagat agacttagtt
    18841 ttaccaagtg acaataattc ttatgaatat atgggaatgg aagtcagcag gcctgccatg
    18901 aaaggcaagg atgagcaagg ctattattat tacacaattg aaattgtggc gaaaatcgta
    18961 atagagagga acaaacaatg acaagacaaa aaaacgccct acgtggccat tttgtagctc
    19021 catacaatgg aggaactgaa ccaacaacag aagatacatg gttggaactt gctaaatgga
    19081 tctcagacgt atcagatgat acagacgaga aaacagatga tcaagcatac tatgacggtg
    19141 atggagttga agaaacaacc gtggtcagcg taaaaggtgc ttataccttt gagggtactt
    19201 acgatccaga cgataaggca caggctctta ttgctgggat gaagtacaag acaggggatg
    19261 accgtaagct atggcacaag gttgtttctt ctgacaagaa gaaacaatgg gtgggagctg
    19321 caactgcaac agaaatcaaa gcaggttctg gcgctgcctc tgactatgag gcgtttggat
    19381 gtaagctttc ttacaactca acgccaaaag agactggtat tgggtaatag cttttgataa
    19441 gggcgggcat tgagccttgc cctttttaac aacaggaaaa ggagtaaaga catgacagat
    19501 attcagattg aactaaaacg tacaggattt ccagtaaaaa tcggagaagt agagctatgg
    19561 tttgatacaa gtcaagagag cttaatgcgc ttttatgaca tggaagaaga actaaaacgt
    19621 cgccttgtcc aatatgaatt agatgtggta tctgcaaata tcaataacaa aattgagcgt
    19681 gatggagtaa ctaaagaagt agttgctggg gctattgaat tggagaagaa acagcttgag
    19741 attcaatatg atcttatttt tggcgacggt acatttgaca agttatattc tatatatcca
    19801 gattataacg ccctaaataa cgctttagaa cagaccgcaa tcatgttgca tgacaagttg
    19861 gaagaacttg ctgagcaaca caaaacggtg gtgaaagagc gtgctagtca ctatttaaac
    19921 aagggaaaag tcactccaat caagaacaac aagaaacaaa aaaagaacaa aaagaaatag
    19981 caggtaaaaa atatgtctat gaaattaaat gatgccttaa tcacaaattt ttctattgct
    20041 gataaagagt acgacataga cctgtctttt aataaagttc tagatgtctt tgaaatccta
    20101 aaagaggatg aaatgacgcg tctagaacaa gctcagttga ttgtccattt gctaactggc
    20161 caagaattat acgacatcaa agaggttgta gattgttgga tttacataaa agaacacttt
    20221 ctagggatcg aaaaagaaac tgttcagtat gatttactag gcaaccccat gccaaaggca
    20281 aaaggtgaag aagaacaaga aaaattgatt gattttgaac aagatgcaga gtacatttat
    20341 gctagttttt tacaagccta cggcatcaat cttttgaaag ttcaaaatga gttgacatgg
    20401 acagaattta aagcactttt gaacgctttg ccagataaca caatcatgca acagattata
    20461 gaaatccgag catggaaacc agaatatggt ggggataaga ataaaatgcg taaattacaa
    20521 gctaaatata gtttaggaaa ggagggagaa gataatggct gatggaaaag tgaccatcgt
    20581 tgtcgatgtg gatggtaata aagtcaaggt tctaaacgat gagttagata aagcggcaca
    20641 gaaaggtgac agagggagcg attctctaaa gaagtttgcg attggtggtg ctgcttttaa
    20701 actggcatct aaagcggtag atcttctaac agattccttg ggcggagcga ttcaacgttt
    20761 tgatactctt gaaagttatc caagagtgat gcaagctatg gggcatagta cagaagatgt
    20821 cacgcgctca actaagaaac tagcggcagg tattgagggt ttgcctacga ctttaaatga
    20881 agtggtaggc acagctcaac gccttacctc gattactggc gatataaaca aatcaacaga
    20941 tttaacactt gctcttaata atgcctttct tgcatctgga tcttctagtg ctgatgcaag
    21001 ccgtggttta caacagttca gtcagatgtt atcagctggt aaggttgaca tgcaaagttg
    21061 gaaaacgtta caggaaacca tgccttatgc tttgcaaaag actgctgatt cattcggttt
    21121 tgctggccaa tctgctcaga atgatttcta ttctgcatta aaagaggggc gtatcacttt
    21181 caaccaattt tcaaagaaat tagttgagct aaacggtggc gtgggtggtt tcgctgagct
    21241 tgcaaaatca aacagtaaag ggatccagac atcttttggg aacttaaaga atgcggttgt
    21301 taaaggtgta gctaatacta tcaaggctct tgacgattta acaaaggcag caacaggtaa
    21361 gacgattgct gagaacttcg atgcattgaa agtaatcatc aatgcggctt ttggtgttat
    21421 tgtcaacgta attaaagcta gcacacctgt ttttcagact ttgtttagta ttttgggtac
    21481 tggagcttct gtaatctcat ctttgacacc agttattatt agtttggttt ctgctttggt
    21541 ggctatgcgt gccgctaatg aagctataac agcaacaaaa aacttaatta attcctggca
    21601 gacattcaaa acaacagcca caggagcgat tcagatcatc aatctaatga cagctgccca
    21661 agctacttgt ggctcagtaa caaaggctca actggttgct aacttggcca ataacggagc
    21721 tttgacagca tccaatttgc tttatggggt tctaactggc tctatcagct tacagactgc
    21781 tgctactatt gctgcgactg ctgcaactac cgcatttaaa gcagcactga ccgctttaac
    21841 tggcccgatt ggtttggttg ttgctggtgt aggtcttgct gttggggcat tggtagggtt
    21901 gtggcaatgg ctaactgccg agagtgagga gaccaaacgc ctcaaatcag aacaagagga
    21961 gttagtcaag agtacggatc aattaacgga ttctgttaaa caaagcgcaa aagaacgtca
    22021 aaaaaatctt gagtctgtaa aaggtaatac agaatcttac caaaaattgg ctgacgaaat
    22081 tgtccagtta tcacaaaaga caaataagac agcagcagac aagaaaaatc tcaagaaaaa
    22141 gattgatgct ttaaatgcct ctgttagtgg attgaatcta gtctatgaca aaaacactga
    22201 ttctttgtct cataacaatg accaaatcaa agctcgtatc tcagcgatgg aggcggaatc
    22261 aacatgggag acatcccaga agaacctgct tgatatcgaa caaaagcgtg ctgaaattgg
    22321 cgaacagcta aagcagatag ctgaacaacg caaaaaatgg aatgaagaat ccaatgttag
    22381 cgatagtgtc cgtaaagaaa gactgcaaga actcaacgac aaggaaactg agctaaaaaa
    22441 tactcagaca gaattgcaaa ctgagtacga aaaaacgtct caagttcaac aggcggcatc
    22501 tgaagcgatg gctgctgctg ccgaaaatgg atctaatcga caagttatat catacgaagg
    22561 tatgtctaaa gctcaacaaa aagcggttga tgatatgcgc tctaaataca atgagttgct
    22621 tgaaaccaca acgaacatgt ttgatcagat acaaatgaag tcagctatta gtgtcgatga
    22681 aatgattgcc aacctccaaa aaaaccaaga ggcggttaat aattgggcaa caaacctcaa
    22741 tacattggcc gaacgtgggg taaacgaggg gattttagct aaattgcaag cgatggggcc
    22801 tcaaggtggg ttgtacgttc aagaactcgt taatgcatca gacgaaaaat tggcaacatt
    22861 gaacgaagtc tttactcaag gtggtgagtc agctatgaat ggcttaactg ctggtatgga
    22921 tacgggtgct ttgggtatca cagacaaaat caagggtatt gtacaaagtc aagtttcaag
    22981 cttacaagag gaaattgcag ctgctgactt ccctgaaaaa gggaaaaata ttcctgaagg
    23041 tgttggtgat ggtataaaag ctggagctga aattgcaagt gaagcttcta aaaacatggc
    23101 aaatgacata aaagaatcct ttacaagtga aatggatatc aattccccat ctcgtgtttt
    23161 caatgagtat ggtggtttta tcactactgg tttagctgag ggggtagata aaggtaccaa
    23221 tcaacctgta tcatctgtta ctaatttagc caatcaaatt aagaaaccat ttgatagtct
    23281 gcagagtgat ttcacgtaca ttggtgaaat ggcgatgtct ggtcttaatg cagggctttg
    23341 gagtggctct ggttctgtta tggcaacagc taattcaatt gctgaaaggg taaaagcgac
    23401 catcaagagc gcactagata ttcactcgcc atctagagca atgcgtgatg aagtcggacg
    23461 tttcattcct caaggtatcg ctgttggtat tgaagcagat gcaggggttg ttgaaaaatc
    23521 aatgttgcga ttaaaagaaa gcatgatgat tgatactaga ccagaaattg cacttggctt
    23581 aaacaagaaa ctaggtgctc aagtgactgt taaacaaagt agtaagcaga caatagctga
    23641 aaaaatcaaa gttactatgg acaagtctag cgaactactc gaaaaagccc tggatgtagc
    23701 tgagacggcc gttagacgac caaatgaaat gtacttaaac gatggtactt tagtcgccag
    23761 aacaggcgat aaatttgcta aatatcaatc ggaacaacta agacgagata ataggatgaa
    23821 aggggtattg tcatgacaaa gataatgact ttcaacggag ttgatatgtc taaattcttt
    23881 cgtataacag atattatccg ccctatcggg aacaagagga gcgtatcaac tgataacgct
    23941 cccttattgg gcgtgaatat ccaacaggtt aagattggag aaaaagagca tatcataaaa
    24001 tttgacatca aaaccacaaa tgcaattgaa atggaacaat taaagcatga tttggcaggc
    24061 attctaaacg ttttagagcc agtaaagatt acttatggcg atgagccaga caaatactat
    24121 atggggttgc cggtagatga gattactcca gaaaatttga caagatggtt ccagcgctca
    24181 gagttaaaaa taataattcc tgatggcgtg gctcacagca cgactttaaa aaattttgat
    24241 atcgatacaa atgaaacaag cgcaccggat aggatagtat ttaatttaac aaatacagga
    24301 acagagccag cttatccaat tattagaatt aaacacaact cagagaatgg atatattgga
    24361 gttgttaaca acagagcggc gtttgaatta ggaaatcgtg aagaggttga tactgaaaaa
    24421 tacagagact ctgaaacatt gatagattat agagggacta atattctaaa aggatttcaa
    24481 aatggcacta aaggagtagc tgtaacaaat gataataagg agcgtcttgt tggcactttg
    24541 agtacaacaa gtatgtgggg gcgtaatcat atcgaattat caaaccgtgg tacagttgaa
    24601 aaaaaccgaa ataatgcaca aagtttgaca tgggctattc ctgttgatag tagtggagaa
    24661 gttggttcat tgaatgacta tctgctttgg agacaagttt ttatggcagc agtcgctaat
    24721 caatatggtt tcataaaggt tactgtatcg gatacagacg gcaatttctt gtatggggtg
    24781 gaaacttaca aacgctatca gacacttgat tgtgagtata gttttttcac cactgacggc
    24841 aaaggtggat acaagtttat caaatggtgg tattttactg ggacaggggc tcaagtaggc
    24901 aaacttgatc catttagcgc ggaaaagggc tggtcagagt taaaaagaaa cgatgataga
    24961 gttcaagtgt tttttgatgg ctctcattat gactttatta ttccggagat aaaagacaaa
    25021 aaatcagcaa aaatccatat cacgcttgga gcactcagag actggcctct tgtatcacat
    25081 atgtatgttg atgagttcat gtatagaaaa gactttgtga caaagagtag agatattcct
    25141 aatcgctatc caataggttc aaatgttgta atcaacagtg aggacgatag tgtgtatatt
    25201 gacgggatat ctaaagtaag cgaagttgta gacggttcac attggccagt aattcctcca
    25261 ggaaaatctc aactagagtt gtatttttca cgttttgtta agaaaaaacc aacagtgaca
    25321 attgaattcg aagaaaggtg gctataagat gcttttaaca attcatgatg caaacttaca
    25381 aaaggtagca tttattgata acgaaaaaca aggcacattg aattattatg acgacacatg
    25441 gactagaagt ctgcctacag gttcatcaac gtttgagttt acggtattta aaaaggctgt
    25501 aaagtctgat ttacctcttg ctaaagccta tcatcatttg aatgagcatg catttgtctc
    25561 atttaagtac aagggtaaaa gctttgtgtt taatatcatg attgttgaag aaaatgagca
    25621 gacaatcaaa tgttattgtg aaaatctcaa tcttgagtta atcaatgagc ttgcgaaccc
    25681 ttataaatct aacaaagcga tgactttcaa agagtattgt gaggcgatgg atcttttaaa
    25741 ttatactcac ctttctattg gtatcaatga aatatcagat tataagcgta ctctggaatg
    25801 ggaggggcaa gaaaccaaac tagcccgtct attaagccta gccaaacgat ttgatgctga
    25861 gattgaattt gatacacagt taaatgctga tagcactatc aaaaagttta gtgttaatgt
    25921 ttatcatgaa aacgatgaca accatcaagg ggtgggacgt gtaagaaatg atgtcattgt
    25981 taaatacgga aaaaacatcc actctattac aagaaaagtg gataagactg gtattttcaa
    26041 tacaatcaga ccgactggta aaatgccaac ggttgaagaa gaaccgagcg gagataaggg
    26101 ctccaaaagc gaaactgtaa aaaatgcaga tggttcaacg acgaaaacca caatctctac
    26161 agcctcagat gggactaaga gcaaaactat tgtccacact aaagttacaa agttagcgga
    26221 caagacacgg atcacaacga ccacaacgac tcgttctgat ggttccatag aacaaactgt
    26281 tacaaccagc aaaaaaggcg gagcatcaac gtctgaaaca aaagtcttga aaaaaccaaa
    26341 tccaaaagaa aaaacaaata caactgagga tgttttgacg attgagggat tggatgaatg
    26401 ggaagtaaag aacgagaaag ggatagttga attttatcaa agagggcaag cactgtatgc
    26461 gcctatttca atgcaactat atccctcaac ctttactcat tcaacagggg agcttgacca
    26521 gtggacaaga aaagattttc attttgaaac agatgagcca aacgagttaa gacgtttagg
    26581 ttatctcaaa ttgaaaaagt attgttatcc agctatcact tatgaagttg atggctttgt
    26641 cgatgctgat attggagata ctgttaaagt ccatgatgac ggttttgccc ctctattgat
    26701 gattcaagca cgggttactg atcaaaaaat cagtttcaca aatccagtga gaaataagac
    26761 aatatttgac aatttcaagg cacttgaaaa caaactatca gctgatatcc agtcagcctt
    26821 tgagagattg tttgaagctg ctaaaccata tactatcaaa ttgtcaacgg acaatggtgt
    26881 tatctttaaa aatcagatcg gccagagtct agtaacccca accttataca agggaggaaa
    26941 accagtcgtt gttggtgtta cttggcgatg ggcacttgat ggagaagtaa caacagggat
    27001 gacttactta gttagaggct caaatgtaac tgatacagtt actctgacag ttgcagctta
    27061 cattggaaat aaagaggttg ctgttgatga gatatcgctt gttaatgttg ctgatggaaa
    27121 acttggtaca cctggaactc cagggcgaga tggccgtact ccttatgtcc atacagcatg
    27181 ggctaataat gcaacaggaa cagatggatt tagtcttgat agctcaatca ataaactcta
    27241 tattggtatt tatacagact ttgaaccaaa cgatagcaca gaccctaaaa aatacaagtg
    27301 ggctaaagta aaaggagaca agggagaaaa aggagataaa ggagaaccgg gacaacgtgg
    27361 tttagatggc ttgcaaggtg caagaggtga acaaggatta cctggtcgca atggtgcaga
    27421 tggccgtact caatacactc acatagctta cagcaatagc gctgatggaa ctaaggattt
    27481 ttctgtaagc gcctctgata gagcttatat cggtatgtat gttgatttta atagtgctga
    27541 tagtaatact ccatctgatt acaattggac acttgtaaaa ggagctgatg gcgcaaacgg
    27601 cgtggcaggt aaggctggta cagatggtag gacaccatac ttacacatag cttacgccac
    27661 atcaaataat ggatcacaag gtttttcaac tactgacagt acaaataaaa cgtatatcgg
    27721 aacatacaca gattacactc aggcagatag cacagattac agagtgtata agtggacgtt
    27781 gataaaaggg gcagatggta ctggtatttc taatgtaact aattattatt tagctactac
    27841 agtctcaaca ggtatcacaa gaacaagcgc agggtggaca actacgccac agcctatcac
    27901 atcagacaag cgttatttat ggaattatcg agttgagcta tacacaaacg gtacaagtaa
    27961 gacaacagag cctactgtta ttggtgtgca cggggaaaaa ggagaacgtg gattacaagg
    28021 tttacaaggc ttgcaagg
//
LOCUS       DAVLSL010000011        27366 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLSL010000011 DAVLSL010000000
VERSION     DAVLSL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27366
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.011"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            152..508
                     /locus_tag="VXN30_000375"
     CDS             152..508
                     /locus_tag="VXN30_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1357049.1"
                     /translation="MTILDRIRSLANDRKVTLAELERNLNFSNGSLRKWETSTPSGDK
                     IEKIADYFNVSTDYLLGRTDNPTIANKKEQFFFEGKEVDVEELASTAMRFNGKPLTEE
                     DKKAIQNIIEIYLRKQ"
     gene            530..910
                     /locus_tag="VXN30_000376"
     CDS             530..910
                     /locus_tag="VXN30_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1357050.1"
                     /translation="MTEKEFSQNLGIDIEIFEDGLFPDEAFYIPALKTMFLSDAISDE
                     KRVQVALHEIGHRNHAPDTYQLFREKCELEANRNMIHHLMKAELDIAEDATTFNYLVF
                     MEKYNLKTIADEIMVKEEYLALLN"
     gene            924..1274
                     /locus_tag="VXN30_000377"
     CDS             924..1274
                     /locus_tag="VXN30_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357051.1"
                     /translation="MNIIAIIIIVIFVGGVIGAVIDNQKKSPEQRERELETFRANQEK
                     KKQEKKQNIITCPNCKSKDVTFLQQDKKAFSVGKAVGGAVLTGGVGALAGFAGKKGNK
                     QWHCQNCGNFFETK"
     gene            1393..2520
                     /locus_tag="VXN30_000378"
     CDS             1393..2520
                     /locus_tag="VXN30_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1357052.1"
                     /translation="MWMEELPNGKFKYFERYKDPYTEKLKKVSVTMEKKTPQARNQAA
                     ILLQEKINKKLSTKQVESITFEEIYNLFYKSWAQTVKESTKHNCKSVDKKMKEVIPSD
                     TILANLDRRFLQEAIEKIIESNGYITAKKVRHRLRGIFNYAVQYSYIENNEVDYTTIP
                     QKPKTLEELEKKRNNFLTMQEIKALVDVLNRREYHQKYADMVLVLTLTGMRYGELTAL
                     QLKNIDFENNKIEITGNFDSVNKIKTLPKTTNSIRTIKVSESVIEAIQRQIVRLSERF
                     QPLSSDDYIFCFEKWNQPTTIACFIQILKKYGKQAKIEKNLSSHIFRHSHISFLAESG
                     LPIKSIMDRVGHSNAKMTLEIYSHTTEDMEDKLVNKLDTIF"
     gene            complement(2608..3519)
                     /gene="whiA"
                     /locus_tag="VXN30_000379"
     CDS             complement(2608..3519)
                     /gene="whiA"
                     /locus_tag="VXN30_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1357053.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHLYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(3516..4493)
                     /locus_tag="VXN30_000380"
     CDS             complement(3516..4493)
                     /locus_tag="VXN30_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1357054.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKIFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(4490..5380)
                     /gene="rapZ"
                     /locus_tag="VXN30_000381"
     CDS             complement(4490..5380)
                     /gene="rapZ"
                     /locus_tag="VXN30_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1357055.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(5432..5812)
                     /locus_tag="VXN30_000382"
     CDS             complement(5432..5812)
                     /locus_tag="VXN30_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1357056.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(5823..6410)
                     /locus_tag="VXN30_000383"
     CDS             complement(5823..6410)
                     /locus_tag="VXN30_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1357057.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(6419..7651)
                     /gene="clpX"
                     /locus_tag="VXN30_000384"
     CDS             complement(6419..7651)
                     /gene="clpX"
                     /locus_tag="VXN30_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1357058.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKNENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(7683..7853)
                     /locus_tag="VXN30_000385"
     CDS             complement(7683..7853)
                     /locus_tag="VXN30_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357059.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(7853..8359)
                     /locus_tag="VXN30_000386"
     CDS             complement(7853..8359)
                     /locus_tag="VXN30_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1357060.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(8489..9007)
                     /locus_tag="VXN30_000387"
     CDS             complement(8489..9007)
                     /locus_tag="VXN30_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1357061.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(9503..11008)
                     /gene="lytC"
                     /locus_tag="VXN30_000388"
     CDS             complement(9503..11008)
                     /gene="lytC"
                     /locus_tag="VXN30_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1357062.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(11046..11804)
                     /locus_tag="VXN30_000389"
     CDS             complement(11046..11804)
                     /locus_tag="VXN30_000389"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1357063.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(11903..12580)
                     /locus_tag="VXN30_000390"
     CDS             complement(11903..12580)
                     /locus_tag="VXN30_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1357064.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(12589..13533)
                     /locus_tag="VXN30_000391"
     CDS             complement(12589..13533)
                     /locus_tag="VXN30_000391"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1357065.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWINHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(13715..14227)
                     /locus_tag="VXN30_000392"
     CDS             complement(13715..14227)
                     /locus_tag="VXN30_000392"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1357066.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(14314..15072)
                     /locus_tag="VXN30_000393"
     CDS             complement(14314..15072)
                     /locus_tag="VXN30_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1357067.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            15280..15450
                     /locus_tag="VXN30_000394"
     CDS             15280..15450
                     /locus_tag="VXN30_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357068.1"
                     /translation="MEIKEQTRKLAVSYSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEYKEHHNIDV"
     gene            complement(15573..16703)
                     /gene="ugpC"
                     /locus_tag="VXN30_000395"
     CDS             complement(15573..16703)
                     /gene="ugpC"
                     /locus_tag="VXN30_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1357069.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(16986..17071)
                     /locus_tag="VXN30_000396"
     tRNA            complement(16986..17071)
                     /locus_tag="VXN30_000396"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17035..17037),aa:Leu,seq:aag)
     gene            complement(17153..17728)
                     /locus_tag="VXN30_000397"
     CDS             complement(17153..17728)
                     /locus_tag="VXN30_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1357070.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(17728..18516)
                     /gene="codY"
                     /locus_tag="VXN30_000398"
     CDS             complement(17728..18516)
                     /gene="codY"
                     /locus_tag="VXN30_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1357071.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            complement(18781..20355)
                     /locus_tag="VXN30_000399"
     CDS             complement(18781..20355)
                     /locus_tag="VXN30_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1357072.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            complement(20554..21780)
                     /locus_tag="VXN30_000400"
     CDS             complement(20554..21780)
                     /locus_tag="VXN30_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1357073.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            21973..23289
                     /locus_tag="VXN30_000401"
     CDS             21973..23289
                     /locus_tag="VXN30_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1357074.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            23388..24731
                     /locus_tag="VXN30_000402"
     CDS             23388..24731
                     /locus_tag="VXN30_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1357075.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            24731..25513
                     /locus_tag="VXN30_000403"
     CDS             24731..25513
                     /locus_tag="VXN30_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1357076.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            25639..26721
                     /locus_tag="VXN30_000404"
     CDS             25639..26721
                     /locus_tag="VXN30_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1357077.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDCAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            26714..26917
                     /locus_tag="VXN30_000405"
     CDS             26714..26917
                     /locus_tag="VXN30_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357078.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(27045..>27366)
                     /locus_tag="VXN30_000406"
     CDS             complement(27045..>27366)
                     /locus_tag="VXN30_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1357079.1"
                     /translation="EKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDN
                     GMMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRT
                     KSFG"
BASE COUNT         8212 a         6030 c         5009 g         8115 t
ORIGIN      
        1 gaaaatcttg gcggattatt ctatttgtat ttatgtcagc tggtcaacta gcttacaaaa
       61 taagtataac ggaaaatttt ctatctgtca atcattttag tagaaaattt tacattattt
      121 tgtaaagcct tatttttgga ggtttttaca catgactatt ttagatagaa tacgctcgtt
      181 agccaatgat agaaaggtta ctcttgccga attggaaagg aatttaaatt ttagtaatgg
      241 cagtctcaga aaatgggaaa cttcaactcc tagcggcgat aaaattgaaa aaatcgccga
      301 ctacttcaat gtctctacag attatttact tggtcgcaca gataatccta ccatcgctaa
      361 taaaaaagaa caattctttt tcgaaggcaa agaagtcgat gttgaggaac ttgcctctac
      421 tgctatgcgc ttcaatggta aaccactaac tgaagaagat aaaaaagcaa ttcaaaacat
      481 aatagagatc tatctcagaa aacaataata atcaaaggtt ggattgttta tgactgaaaa
      541 agaattttct caaaatctag gcatagatat agagattttt gaagatggtc tatttccaga
      601 tgaagccttt tacatcccag ccctcaaaac tatgtttttg agtgatgcta tatctgatga
      661 aaaaagggta caagtcgctt tacatgagat aggccataga aaccacgcgc cagatactta
      721 tcagcttttt cgggaaaagt gtgagcttga ggctaatagg aatatgatcc atcaccttat
      781 gaaagctgag ttggatatag ccgaagatgc cactacattt aattacctgg tatttatgga
      841 aaagtataat ttaaaaacca ttgccgatga aatcatggtc aaagaagaat atttagcact
      901 acttaattaa aaaaggagag aaaatgaata taattgctat tatcataatt gttatttttg
      961 ttggaggtgt cataggtgca gtaattgata accaaaaaaa atctccagag cagagagaac
     1021 gtgaacttga aacatttaga gcaaatcaag agaagaaaaa gcaagagaaa aagcagaata
     1081 tcatcacttg ccctaattgt aagtctaaag atgtgacttt cttacaacaa gacaaaaaag
     1141 ccttttctgt tggaaaggcc gttggtggag ctgttttgac tggtggagtt ggtgctttag
     1201 ctggatttgc tggcaaaaaa ggaaataaac agtggcattg ccaaaattgc ggaaatttct
     1261 ttgaaacgaa ataaaaaaat ccccacactc gtcatcgcca aatcttgagt gtgaggaaat
     1321 catgtatagg aaacaaccat tcaaaaggtc gttttcttat acccatttta tcaaaaaagt
     1381 gaggtaaaat caatgtggat ggaagaattg ccaaatggca agtttaaata ttttgagcga
     1441 tataaagatc catatactga gaaattaaaa aaagtttcag taaccatgga gaagaaaact
     1501 ccccaggcaa gaaatcaagc tgctatcttg ttacaagaga agataaataa aaaactcagc
     1561 acaaaacaag tagaaagcat tacatttgaa gaaatctata accttttcta taaatcatgg
     1621 gcgcaaacag taaaggaatc aacaaaacat aattgtaaat cagttgataa gaagatgaag
     1681 gaagtcatac catccgatac catacttgct aatcttgaca ggcgttttct tcaagaggct
     1741 attgaaaaaa ttattgaaag caacggatat attacagcta aaaaagtacg gcataggctc
     1801 agaggtatct ttaattacgc tgttcaatac tcttacattg aaaacaacga ggtcgattat
     1861 actacgattc ctcaaaaacc aaagacttta gaagaactgg aaaaaaagcg taacaacttt
     1921 ctcaccatgc aagaaataaa agcacttgtc gatgtcctta atcgtcgaga atatcaccaa
     1981 aagtacgctg atatggttct tgtgctgaca ttaactggta tgagatatgg tgagttaact
     2041 gccttacaac tgaagaatat agacttcgaa aacaacaaaa ttgagatcac aggtaatttt
     2101 gattcagtaa acaaaatcaa gacgctacca aagactacaa attcaatacg gacaatcaaa
     2161 gtatcagaga gtgtcataga agctattcaa agacaaatag tacgacttag cgaacgtttc
     2221 cagccattgt caagcgatga ttatattttc tgttttgaaa aatggaatca acctacaaca
     2281 atagcttgct tcatacagat attaaaaaaa tatggaaaac aggccaaaat agaaaaaaac
     2341 ttatctagcc atatttttag gcattctcat atttcgtttt tagcagagtc tggcctccca
     2401 ataaaatcaa taatggatcg agttgggcac tcaaatgcaa aaatgacttt ggaaatctat
     2461 tctcatacta ctgaggatat ggaggataaa ctggtcaata aattagatac tattttttaa
     2521 ttctgcccct tggttgcccc tttttgattt cataacacaa caaaacccct tgaagatatt
     2581 gataattcaa ggggttgctt tatatcgtta taattcatcc gctatcttat tgatttttct
     2641 gagtctgtga ttgacaccac ttttggtcag aggggtgctg agactatctg ctaactgctg
     2701 gatagagtag tctgggtgct gaatcctcag ttgcgccact tcctgcaaat ctactggcaa
     2761 attttctaag cccatgatat ctttgatttt actgatattg ttaatggtct tcatgctggc
     2821 agaaactgtc cgagcgatat tagctgtctc ggcattatta gcccgattga ggtcgttacg
     2881 ggtttctcgc aaaatcttaa cccgctcaaa atcatcacgt gcctgcatgg ctcctattac
     2941 tatcaagaag tccataatgt cttctgctcg ctggagatag gtcacagccc ccttcttgcg
     3001 ctcaagcacc ttggcatcca gtaaaaactg ttggagaagg gaggcaattc cttgcgcgtg
     3061 gtccagataa acagaactga tttccaactg gtacttgcct gactcagggt cacgaatgct
     3121 cccatttgcc aagaaagcgc cacagagata ggcacgacct gcttcctcat ccgataaaat
     3181 cgcctcatca atacctgttt ccaggccaaa gaaagagtct gccaagtgca aatcacttaa
     3241 caaatcctgc accttttcat ctgtaaaaac ggtatagacg cgattcttgc gaagattgct
     3301 ccgttggtgg tgacgaattt cagatttgat ttcatagaga tggagaaagg actcatagag
     3361 gtgacgggcc agtttggcat tttctgtcac aactgacaaa gtcaagcccg aagtcgagag
     3421 accgatgcta ccagacattt tgataatggc agataattca tgccagctca gatggtgttg
     3481 gcccaggatt tcttctttta ctgctactgt gaaactcatt ttttcacctg tataatgcgc
     3541 atcaactcgt ccacaatcaa atctccatcg tggaaggcac cgccattttc cagacgaagg
     3601 aagttagatg aaatcacgcg cgaaacttgc ttacaaagac ctacaaaatc gtgttccact
     3661 tgcactaagt attcatcaaa acggttggaa ttcatgtatt cctgaggcac tttttcaata
     3721 ttcaccaaga cagtgtcgat aaaagggcga ccaaggtgac gatgcaagac ttccacgtgg
     3781 tcgctatctg taaagtgttc cgtctcccca cgttgggtca tgatattgca gacataggca
     3841 atttctgcct tggtttccaa aagagcccgc ccaatttcct taatcacgat attgggcaaa
     3901 atagaggtaa agagggaacc tggccctagg acaatcatgt cactttcaag gatggtctgc
     3961 actactcgac ggctggccag aggcgtatca tcgtttaggg cattggtcac atagacattg
     4021 tcaattatgc ctcgatggtc tacaatatga ctctctccag ccacttctgt cccatcctga
     4081 aagactgcat gaagggtcaa aggatggtca ctggaaggat aaattttccc tgttgtatgg
     4141 aaaaatttgc tcaataactg catggcatta taggttgaac cctgcatttc tgacaagcca
     4201 gcaatgatga gatttcccaa tggatggcca gcaaaggctc cggcatcctc agagaaccga
     4261 tactgaaaga tcttctcata aaacttaggc atatccgaca tggccacaag gacattacga
     4321 agatcacctg gcggtgtcaa ctgttgcata ttttttcgga gttcacctga agaaccacca
     4381 tcatctgcca ccgtcacgat agctgcgatt tccacatctt tttcccgcag actttttaga
     4441 atgacgggaa ttccagtccc tccaccaatc accgttatct ttggttttct catgaacggt
     4501 ttaccgtttc ctttctgcgg tctttgtcgc gatgcccttc attaacagac caattcttgg
     4561 ataagtcctg cgccaagcgt ttagcaaatg ccacactacg gtgttgtcca cccgtacatc
     4621 ccatggcaat ggtcaaaacg gacttacctt ccttttggta acttggcaga atcggctcaa
     4681 tcaaggccaa taaatgttga taaaagtctt ctgactcagg atggttcatg acataatcat
     4741 aaacaggttc atccacaccc gtttggtttc tcagttctgg tagataatag ggatttggca
     4801 agaaacggac atcaaagacc aagtccgcat caatcgggat tccatactta aatccgaaag
     4861 acatgacttc gatacggaaa gactgggctt gttcttggtc tgaaaactgc tctgcaaggg
     4921 ttttgcgcag ctcacgtgga gtgagttcag tcgtatccac cacattttgg ctcatatttt
     4981 tcaaaggtgc caagagttca cgttccaact tgattccatc taaaatacga ccgtctgctg
     5041 ctagtgggtg actccgtctg gtctccttgt aacgagcgac caattcctta tcagccgcat
     5101 ccaaaaagag gattttgaaa tccaaaccat cttgattttc caactcatcc aaaacagctt
     5161 gaatctctga aaagaaagaa cggctacgca tatccactac caaggccaac ttaggattgt
     5221 cttccttaat ttcaaccagc tgcaaaaact taggcaagag agctggcggc atattatcaa
     5281 tggtgaaata acctagatcc tcgaaggact gaatggctac agttttccct gcgccactca
     5341 tccctgtcac aatcaccaag tgaagttgtt tctttgtcat ctttttctcc ttatatcaaa
     5401 agaagtttgg caacaccaaa cttcaactag cttatccaat ctctgcgatg acttcaattt
     5461 cgacttttac atcacgagga agacgagcta cctccacagc tgaacgagct gggaattcct
     5521 ctttgaaggc cgtttggtaa acctcattaa aaggaacaaa gtcgttcata tcgctcaaga
     5581 aacaagttgt tttgacaaca tggtcaaagt ctgttcctgc ttctgccaaa atagcaccga
     5641 tgtttttcaa gacttgctct gtctgttctt ggatattctc tcctacaatt tccccagttt
     5701 caggggatag gggaacttga ccgctagcaa acaaaaggtt gccaacgatt tttccttgaa
     5761 catagggccc gatagccttt ggggccttat ctgtatgaat tgtttttgcc attttctttt
     5821 cctcacaatt tttctaagat tgcatcccaa gcctcatcca tccctgcctt gctgacagat
     5881 gaaaagagga tgaaatcgtc acttgggtca aagtttaatt tctttttgat tgctgattca
     5941 tgcttgttcc atttaccacg aggaatcttg tccgccttgg tcgccacaat gatgactgga
     6001 atctcataat acttgagaaa ttcgtacatc tgcacatcat ctgctgacgg gtcatgacga
     6061 aggtcaacta gactgacaac cgcacggaga ttttcccgag tcgttaagta ctcctcaatc
     6121 atgcaccccc acttttcacg ttccttttta gaaacacgag catagccata accaggcaca
     6181 tccacaaagc gcatcttgtc atcaatgtta aaaaagttca ggagctgggt tttaccaggt
     6241 tttcctgatg tacgagcgag attcttacgg ttcaacatag tgttgataaa gctggattta
     6301 ccaacatttg aacgccctgc tagggcaatc tctggcagtt catcctgcgg atagtgggac
     6361 ttattagctg cactgagcaa gatttcagca ttgtgtgtat taagttccat agtcacctct
     6421 aggctgtttc taggatcggt ttatccgttc catcgacagt ttctttagtg atgcgaacca
     6481 atttcacatt ttcctgactc ggcacctcaa acataacatc tagcatggtt tcttcgatga
     6541 tggagcgaag tccacgcgcc cctgtcttcc gttcgattgc tttattagca atctcttgaa
     6601 gggcttcgtc gtcaaattcc aactcaacat catcataaga aagcaaggtt tggtattgtt
     6661 tcaccaaggc atttcttggc tctttcaaga tgcgaaccaa gtcatcaacg gtcaattgct
     6721 caagagccgc aaaaacaggc aagcgtccaa tcaactcagg gataatacca aatttttgaa
     6781 tgtcttcagc gatgatttct tgcatgtatg agctgttttc gtcaatcgcc ttattgtttt
     6841 gaccaaatcc gatgactttt tcacccagac gttgtttgac aatttcttca ataccatcaa
     6901 aagcaccacc cacgatgaag aggatatttt ttgtatccac ttgaatcatc tcttgttgtg
     6961 gatgtttgcg tccaccttga ggcggtacgc tagcaacagt tccctcgata atcttgagaa
     7021 gggcttgttg caccccttca ccagaaacat cacgtgtgat agacacattc tcattcttct
     7081 tggcaatctt gtcaatttca tccacataga taatgccacg ctctgcacgt tcgatgttaa
     7141 agtcagcaac ctgcaagagt ttgaggagta tattttccac atcctcaccc acataaccag
     7201 cctccgtcag agctgtcgca tccgcaatag caaaaggtac attcaagctc ttagccaagg
     7261 tctgggcaag gaaagttttc cctgaaccag ttgggccaat catcaaaatg tttgacttct
     7321 gcaaatccac atcttctgac tcttcgcgtg tatcgtggaa attgatgcgt ttgtagtggt
     7381 tataaaccgc cactgccaag gcacgcttgg cacgatcttg accaattaca tagtggttca
     7441 agatatggag gagttcaatt ggttttggca cctcagacaa gtctgccaag acttcctcaa
     7501 ccaattcttc tcgaatgatt tcctgagcta actccacgca ttcattacaa ataaaagcat
     7561 tgttgccagc aattattttt tgtacttctt cttggttttt gccacaaaat gagcaataaa
     7621 ccatcatatc attttttcta tttgtagaca tgatttcctt ccattctata ctgtcattct
     7681 atctaaaata aggtcatgta aaaagcatga atactattga ccagattggt aaaggcattt
     7741 aaccaaagga ggatagaaag cccgtaacgc tttttacgaa aagcctgtgc tcctgccaga
     7801 aagcagatga aacacagaaa agccgtgaat agaccaaata aactccgttc cattatactt
     7861 cctttctctt gcggtattgg atggtaaaat cataaggatt cttctcatct ttggcgtaaa
     7921 atttgcttga aactgtctca aaaagagaca agtcaagctc ttcagggaaa taggtatctc
     7981 cttccacccg agcatgaatg tgagtgacaa tcacttcatc aaggtaaggt tcaaaagcct
     8041 gaaaaatttg cttcccaccg ataatgtaga gattcttttc ttgatcctga taccagtcaa
     8101 gaacagactg gacgtcctga aaagtagcaa ccccatctat cttttcttcc ggattacgcg
     8161 tcaaaatcag ggtttcccgt tttggaagca agcgacgccc catcccatca aaggtcacac
     8221 gccccatcaa gatagcatga ttcagagttg tttctttaaa gtgctgcaat tctgctggca
     8281 aatgccaagg cagacgattt tccttaccaa tcacaccctc ttcatcctgg gcccaaatag
     8341 ctacgatttt cttagtcatg cttccatcct tttcactgat agtactattt tatcaaaaaa
     8401 ctcaaaaaaa gactggtttg gaatagctta caaaatagaa aaaatctgta agaaatttcc
     8461 tacagattta tctatgtttc cttatttctt acaaaccagg tgcttgtcca agttcggctg
     8521 caagcatcca aattgtttta tctgtttcag ttttagcgcc tgcaaagata ccgtttgtca
     8581 catcgtcacc ttcttcatca gtgacatcca aacctttttg gaaaagttct gacaagtaac
     8641 ggtagataac aagaacacgt tccaagcttt cttcaacatt acggtattca ccagcttctt
     8701 cttcgatttc actattttga aggaactctg tcaatgtaga gaatgggctt ccaccgagtg
     8761 taatcaagcg ttcgctgatt tcatccaatt gaccgtcaag agcttccatg tactcatcca
     8821 tttttggatg ccatacaagg aaaccacgac catgcatata ccagtgcact tggtgcaaag
     8881 caacgtgagc tacatacaaa tcagcaacag cttggttcaa gacttccttt gtttttgcca
     8941 atgctactgg cgctgctttt gtcaatgatg ttacgtcttt tactgcttct tttttcaatt
     9001 ctaccatttt ctttacctca ttcattattt tttgtaacca cttcttaatg attactacct
     9061 tagtatacta ctaaggaaaa ccaatagcaa gacaaagaac ttatatgaga aaagttgcaa
     9121 tagactgata atttaataat tctttagaag acaaaaagag ggttggatgc ttatagtcaa
     9181 tggaaatcca tcagcaaact aggaaactaa acccagcttg accaaagcac aagttttgag
     9241 gtcgcaggta gaactgacca agctgatgtg gtttgaagaa attccccaat actataatag
     9301 attgaaataa gatgaaacca agtcgattag gaaaggtaaa tgaatttcta aaatgtttta
     9361 ccaatcacaa ggtatttgtc tatattcaat ctactatata agcttcattt ccgagcaaga
     9421 tcttgaattt actcgaggat gaactatcat aaaacaaaga aaaagagggt gctactatca
     9481 catccctctt tcaaatcatc gcttaatacc aaacgctgac atctacgcgc ccttggattc
     9541 ctttcatgta ttcagaagag gtatattgcc aacctttttc tcctgaataa taagggtttt
     9601 cccattctaa agcattcgta taggccgcta cccagtttac atgttttaaa atatctgggt
     9661 gttttaaacg cgtctgtaat aaactacgat agctatagac atacacattt tgataacccg
     9721 cctgcttcat cgtgtccatg tacttgttga tgattttaac ccaagtgcct gtatcacttg
     9781 gagctctctt gctcttattt acatattccc aattctcaac atcataatag atagggtaag
     9841 acaggttcat attgtatttc tttataagtt caatggtctg tttagcgtca ctctcagcat
     9901 cggtctcatt ttcagcatag gtatagagat agacaccata aggaattccc agacggttta
     9961 actccttaat gttatgcgcc aattccttgt cttctttacc gctataacct agacgaacaa
    10021 tgacaccatc cacttcgttc tcatcaataa cctttttcca atcattgata cgaccattgt
    10081 gctcactaat atcaatgact ttcttagcat gttcggttcc cacttgttct tctctattat
    10141 taaagaaata gcgtttacca tctctgtgaa cccagccgac attcaaatct ttcttttctt
    10201 tgagctcacc agaggccgca aagatatagc gagcaccatc cataatcact tcgtcagtcg
    10261 ccatagcacc acttccagtc aaatagtagt tgccttgcca ctcattccga gccatatagc
    10321 cccacttctt gaaatagtac catttgccgc ccactttttg ccactcttgg ttagcataag
    10381 ttccatcgga ttttagataa aaataagcag aataggctgg atcatagagc cattcatttt
    10441 gcatcatagc tccttgacca ttcaagaaat aacttccttg ccattggctt ttagccatgt
    10501 aaccccactt cttgaagtag taccacttat ttccaatcaa ttgccattct tgatgagcat
    10561 aagccccatc tgacttgaga taaaaccaac tcttgtaatt actgtcatag atccactcgt
    10621 tttgggccat atatccacct gatttcaggt agtaatttcc atgccattca ttctgcgagt
    10681 aacgaccatc tgaattaata taaaaccagg ccttatagta gttgtcaaaa atccattcac
    10741 tttgagcttt agagccatca tcctttacat aataatcccc atcccagtgg gcagcagcat
    10801 tagtctgtac ttctgtgctc gtttgcgttt tattagaaga ctgattttgc tctgaactac
    10861 ttgaagctgt cgttgtatcc tgcgaagttt ttgctacttc agtttcattt gcagcgacat
    10921 gactagttgc caagcctagt aaacagatac ttgctaatcc aatttttatt ttcaatcttt
    10981 cctctcctat aaaaaatgta acagacatct gaatgctgtt ccacctagct tttgctactt
    11041 actgattatt ttacaaagtc aagcaaagcc aagaagcttt cagcttcaag tgacgcacca
    11101 cctacaaggg caccgtcaac gtctgggcaa gccatgtatg aagcaacatt ttcaggttta
    11161 acagaaccac cgtattgaac acgaactttg tctgcgactt cttgaccaaa gtcagcagct
    11221 acaacgtcac gaacaacttt acacattttt tgtgcatcgt cttgtgaagc tgatttacca
    11281 gtaccgatag cccagattgg ctcataagcg ataaccgagg cagcaacttg ttcagcagtc
    11341 aatccagcca atgcagcaga tacttgagca cctacgaatt cagcagcttt accagcttcg
    11401 taagtttcaa gtgattcacc acaacagatg attggaagca taccgttcgc aaagattgct
    11461 tttgcttttt tgttgatatc ttcatcagtt tcatggaagt agtcacggcg ttctgagtga
    11521 ccgataacaa cgtagtcagt accgatttct ttcaaaactt gtgggctagt ttcaccagtg
    11581 aaagcacctg cattttcaaa gtagcagttt tgagcagcaa ctttaaggtt tgagcctttt
    11641 gcaacagcaa gaacagttgt caaatcaaga gctggagcag cgatacctgc ttcaacaaga
    11701 tctgatgaag gaagttttga tgcaactgct tcaacgaatg ctttagcttc ttctggattt
    11761 ttgttcattt tccagttacc agcgataaat ggtttacgtg acatttcaca tacctctttt
    11821 ttcaatttat tttctatttt attctatcac aattatacac agcttgcaaa tcttactcgg
    11881 atttcaagcc tgcttgcatg gattaatcct tccagagatc catggcattt ctgaaatctg
    11941 cagatacctg tgtcaactga ggattgcttg cttctctttc tgctctcttg gcctcaattt
    12001 gagccacact cttgattcct tcatggcgcc agtttctcaa aatcgcctga atgtacttcc
    12061 agtttggttt tccattcaaa acagcttcac gaagagcctc cttaatcaag tcagcactgg
    12121 ttccatcttc ctttagtgtc ttggtcaaat cctcaatctc aaaaggcgtc aacaatcgtc
    12181 ccaactcctg ctggaaggtt tccaccaaat ccttgagctg gttttgaggt gtcagctggt
    12241 ctgaacttga atgaactgct ccaaacaggt catccaaacg ttccaaggcc aaactagcat
    12301 caaagagcaa ttcaatttcg ccatttaatt cgatagtacg atactggagc agtccccttt
    12361 ccgtcagatt agaaatggac tggttgacat ccgaaatttc cttgccaatc ctttcagcaa
    12421 tctggcttgg cgacatttct tctaagcctg tcgtattttg caaatagaaa aattgccaaa
    12481 ccagaaaatc gtcgctagaa ggaaagagtt ccttaaaatg caagagcagg gcactcggta
    12541 aaaccaagat acctgattta aaagcgtcta aatatgtcat aattcctctt ataaataccc
    12601 atatgcagat gcatcatctt ctatctttct ccaatcaaaa ggcgtctcct gatagaccgc
    12661 atggtttatc caattactga aaaagagggc tgcagatgaa gaccaacaaa gacaaggtac
    12721 ctgattaaca tcatcatctt taaagtaatt ttctggaata tgtgggtcga aacctgcatc
    12781 acgatctcga aaatactctt ttgccaaggt atcacggtca tactccaaat gaccaaaact
    12841 ataaatttct cgtaaatcac gactggccaa aatagaaacc ccaacctgag gtccttctga
    12901 taaaatctcg agattggtct tgtttaagac ctcttcctta gaaatctccg tgtgccgtga
    12961 atgaggggat acatagctat catcaaagcc tctaaataga aggtgaccct cttttagggt
    13021 gtcctgagga taaatacctg atagcttact gtccatctgg tatttttcta caccatagcg
    13081 cagataaagc ccagcctgag ccccccaaca gatatgaagg gtcgaataga catgagtctt
    13141 agaccactca agcatctgtc taaattcctc ccaatagtcc acttcctcaa atggtaaatg
    13201 ctcaactgga gcacccgtga tgatcatccc atcaaaatac tcatccttga cttcaggaaa
    13261 agttttatag aaggtctcca tgtgctctga acgagttgtt ttagaacggt ggctctccat
    13321 atagagaaaa tcaatatcca gttgtagggg tgtattagcc aagtggcgca acaactgggt
    13381 ctctgtgacc attttctgtg gcatgagatt taaaattaaa atcttcaaag gacggatatc
    13441 ttggtgggca gcacgttgat catccatgac aaagatattc tctgtccgta aaatctcaac
    13501 agctggcaat tttttatcaa ttcgaatcgg cataaccctc tcctaactgt actctttcag
    13561 gaataattgc atatgtttga agacaaatct caaacactta gtccttttat tatactgtaa
    13621 gaagatatag ttttcaatta tagtttttct ctaactagtt atagtctatt tttatatcct
    13681 agtgtagaga aaacagccct agggactgtt ttcattaata atgcataaga actttgtagt
    13741 cgtagtcacc aattttttca cggccgttca attcatccaa ttcaacaagg aaggcacaac
    13801 ctgccataac accaccaagt ttttcaatca tctcgatagt tgccttaaca gttccacctg
    13861 tcgccaaaag gtcatctaca ataagaacac gttgacctgg cttaatggca tccgcgtgca
    13921 tagtcaaggt atcgacaccg tactcttttt catagtcagc agaaataact tcgcgtggca
    13981 atttacctgg cttacgaaca ggcgcaaaac caattcccaa ctcaaaggca actggacaac
    14041 ccacgataaa tccacgagct tcaggtccca cgatcatgtc gactttcttg tcagtagcat
    14101 actgaacgat ttcacgaaca gcgtagctat aagcatttcc atcagccatc aaaggactaa
    14161 tatcacggaa ggtaatgcct tcctttggat aattttcaat tgttgcaatg taatctttta
    14221 aattcatctt tttctttctt tcaaagtttt ttactctcta ttatagcata ttttttaaga
    14281 aagaaaaaag gaaaagttaa cttcaataac tatctaacgt tttgacgatt tataactagc
    14341 catcgcaata aagcccaatt tctgtttatt cttagcaaac attttataca tagttaaaaa
    14401 ctgctttcta ttctcctttt tacaagcatt tacacaaatt ttcaaagttc ctagcaaacc
    14461 ttcgtcataa atcatacccg ataatttcat taatgtcatt tcaccagtca atgctttcac
    14521 atcacaataa cctgattcta tcatcacctg ttcccaacca tcttgagtta aaggacctac
    14581 atttacatga attgcttgtg ataattcctg tctgatagac tctttagctt ccttaagaag
    14641 tacatcatgt gtcaagagaa gacctccagg ttttaatacc cttagatatt ccattacaca
    14701 ttttttctta gcttgatcgg cttgcatagt cagcatagct tcatttataa caatatcaaa
    14761 actagcatct tgataaggaa gtttcattgc atttgctctt tcaaaactga ttaaatgagc
    14821 aacacctgcc gttccagcag attttttagc cacttctaaa gcttgagcat ccatatcaac
    14881 agcagttatc ttgcaaccaa aacgctgtgc caactcaatt gctgtagttc ccctattaca
    14941 cgcaacctct agtattctct tttcttttga aaatcctcct tctgcaatta accaatctgt
    15001 ggcacgcttt ccacctggac gtaagcgttt tttccccaat tttgctaaaa acttatgacc
    15061 tgcttctgac atttgaacac ctctgttttt tcttcattat aacacaaaat gatattatct
    15121 gacaattcta atctattatt tgataaacta gaatcaaaag catacaagtc accataacta
    15181 tagcataaat ttattattac ctctctagtt cgaattcaat tgctcacgta gtttacgcat
    15241 atagcttcac ttgtagatgg tacccacaaa taatactcaa tggaaatcaa agagcaaact
    15301 aggaaactag ccgtaagcta ctcaaagtac agctttgagg ttgcagataa aactgacgaa
    15361 gtcagtaatc atacgtacgg caaggcgacg ttgacgtggt ttgaagagat tttcgaagag
    15421 tataaagagc atcacaatat agatgtataa tatgacttcc ctagcaaaaa ggagagaagc
    15481 attttgtttc tctccctaga ttaactgata tctctccact acaattgata aagagatatc
    15541 ttttcttgta gtgctttgaa ttttatttat tcttagtaga ttgttttttc agtttctaca
    15601 tcgaagaagt gtgctttgtt caagtcaaat ccaagctcaa ctgttgcacc tgtttgcaag
    15661 tagtcacgag catcaacttt tgcaacaaac tcgtctttac caacttggca gtaaaggtga
    15721 gattctgaac caagcagttc tgatacagag atagtcgctt ttacaacaca gtctgggaat
    15781 gtttcaagga aagcaggttc tgcattcacg tcttctggac ggataccaaa gatcaattct
    15841 tttccttcgt agcctttttc acgaagaact ttcaatgctc cttctggcac tttcaaacgg
    15901 aaaccgtcag aaacaatttc gctaccaacc aatttcacgt tgatgaagtt catagctggg
    15961 cttccgatga atcctgcaac gaatttgtta actggatttt tgtaaacttc ttgaggagta
    16021 ccgatttgtt ctacacgtcc gatagtacct gtaccagcag ggttcttagt agctgacata
    16081 ataacgatac ggtctgcaag tgtcatcgct tctgtttggt cgtgagttac atagatagtt
    16141 gtagctccga tacgacggtg aattttagcg atttcagcac gcattgatac acgaagtttg
    16201 gcatccaagt ttgacaaagg ttcgtccatc aagaatactt tcgcatcacg gacaatcgca
    16261 cgccccatgg caacacgttg acgttgacca cctgaaaggt cagccggttt acgttccaag
    16321 aattctttca atccaagtat ttcagctgct tcttgaacac gtttgttaat gtcttctttg
    16381 ctgtatttac gcaatttcaa accgaaagcc atgttgtcat aaacagtcat gtgtgggtaa
    16441 agagcgtagt tttggaatac catggcgata tcacggtctt ttggagctac gtcgttgaca
    16501 accacgccat cgatagatgc agtaccttct gtgatgtctt caagaccagc aatcatacgg
    16561 agtgtagttg atttaccaca tcctgaaggt cctacgaaaa cgataaattc tttatctttg
    16621 atgttcaagt tgaaatcttc aactgaatag tgttcgctgt ttggatattt tttgtaaata
    16681 tttttaagat tcaattctac catgaggtga actccttttg tcttttgata attatattat
    16741 agatgaaaac gctttatatt tctatcgcaa gatgaccaaa aaaataaaaa agttctgtgc
    16801 aacttgcaca aaactttaga gaatatcgga gaatttgaag aagataatct ttattattct
    16861 tctatgacaa aagcagaaac aaggatcagt tttcccatat tttcgctgat tctccactac
    16921 atttgactgg attctaattt tttagagaaa tacaaaagag ctagctttag ctaactcttt
    16981 tcctatgcgg agagagggac ttgaaccctc acgacctaaa gcggtcacag gatccttagt
    17041 cctgcgcgtc tgccaattcc gccatccccg catcgattac tttactagta tatcaacttt
    17101 tgggatgctt gtcaacactt tttttcaaat tttttcattt tcaccaacca ggttactcag
    17161 aaagttcatt tagattttca tctactaact tagctccgag tgtatttttg aaatgaccta
    17221 gggcaaattg atgattttca ggccagatgg aagcaacagc tggtttaaca atctcgatgt
    17281 catatcctag attataggca tctatagctg tatgtaggac acagatatcc gtcaagacac
    17341 ctgttaagat aacggtagac actctacgct ctctcaaacg aatatctagg tcagtccctg
    17401 aaaaagctga gtaatggcgt ttatccatcc aaaagacacg actgtctgaa ccatgctctt
    17461 gataaaagat ccccaaatct ccatataaat tccgtccact cgtcccaatc agattatgag
    17521 gaggaaataa cttactttcc ggatggaaac aatcgttttc ttcatgagca tcaatagtaa
    17581 agaagatata atctcctcgt tcaaaagcta atcgagttac cttgctgatg gcatccgaaa
    17641 tcgcctgagc tggagcacct gctgtcagct tcccactatc agcaacaaaa tcttctgtat
    17701 aatcaatcga aattaaagcc tttgtcatta gtaatctctt ttcttcactt cttcaaaaat
    17761 atctgaaatc aagaccttaa gataggttcc cttcattcca agtgagcgac tttcaataat
    17821 ccccgcagac tcaagtttac gaagagcatt gacaatcaca gagcgagtga ttccgatacg
    17881 atctgcaatc actgacgcag tcaacttccc ttcatttcca tttaattccc ctaaaattgc
    17941 tgaaacagca cggagttcgg agtaagaaag ggtattgacc gccatggtga cagcagtacg
    18001 acgacgaata tttttctcat cttcttcacg ttggaagtta agaagctgaa tcccaacaac
    18061 ggtactggca atctcaacaa gaaccaagtc ctcatcttcg aattttttat cattacgcca
    18121 aataatcaaa gaaccaaggc gaatccccga tacatgaatc ggtgcaatag tcgtcaagcc
    18181 atctggaaaa tcatctctac tctcaacagg gaaaatactc atatcatgct caacaggcaa
    18241 gtttgcttct gtttcgtaaa tcatattagc cccttgaacg tagtcatctg ggaaaatctt
    18301 agtttggaag aattgctcta cgcgatctgt atttgtttta taacgcataa aatagccaag
    18361 cagacgtccc ttactattga taatgcaggc attgcaatga ataatatccg ctaactgacg
    18421 cgtaatagcg ttgtaaggga gctcatcctg caactgctcc tctgagcgtt tcaaaattga
    18481 tgtaattttt ctagtttttt ctaataaatg tgccattttt cacctcgaat ttaatcgcta
    18541 tcattataac ataaaaacgt ctctttttca ataattatct gaaaattcct tattgacttg
    18601 cattgactta caatttaatt aaaaaccaga atatttttaa ttaaattgtt ccttttctat
    18661 tgacaagtcg cctatttttg tgtatcataa tattataaaa gataatataa taattttatt
    18721 tgtctttttc acattcggtc tccttatata aaaaagcgat tcattttgaa ccgctttttc
    18781 ttatttatca cctttgttac gaataacaaa gcctgtttgc ttttcgctta aagtattgcg
    18841 tggtttttta ttatccttac ggtaacgttt ttccttatca aaacgatcgt tgccacgact
    18901 tccttttttg aactcatcac ggcgaccatt gccacgacga tcacgctctc gacggtcgtc
    18961 cccacgacgg cctccacgac ctcccttagc tttaccaccg aaaccattac ctgatggttt
    19021 aaacggtagt ggtttttcac gtgcaatctc cacttctgga aggctatctg ggtcttggac
    19081 tgtcagactc aagatataca ttgccaattc ttctggagta aactcagcag ccaatttgcg
    19141 agcatcctta ccaaatttct caaagttggc acgaatggtt tcatctgcaa aatcacgttc
    19201 gattttcttg agagctacct gtttttttga ttggaaggat tcttctacac ttgcaggttt
    19261 gagacctttc atgcgtttct tagtcaagtt ttcaatgatt tgaaggtaac ccatttcgtt
    19321 tggagcaaca aaagtaatag attgacctga cttaccagca cgacctgtac gaccgatacg
    19381 gtgaacataa ctctcaggat cttgtggaat atcgtagttg tagacatggg tcacacctga
    19441 aatatccaaa ccacgcgctg caacgtctgt cgcaaccaaa acatcaagat tgccattttt
    19501 aaagtcacga aggacacgaa gacgtttgtt ttggtctagg tcgccatgaa ttccttctgc
    19561 acggaagcca cgaattttca aaccacgagt caattcatcc acacggcgtt tggtacgacc
    19621 aaatacaata gcgagttctg gttgtgccac atccatgaga cgagtcatgg tgtcaaattt
    19681 ttcttgttcc ttaacacgga tatagtactg gtcaaccaat tctgttgtca attccttagc
    19741 cgcaatcttg acatgttcag gggctttcat aaactgaaca ccgatacgtt tgatggcatc
    19801 tggcatagtt gctgagaaaa gcaaagtttg acggttctca ggtacacggg aaataatggc
    19861 ttcgatgtct tcaaggaagc ccatgttaag catttcatcc gcttcgtcaa ggataagggt
    19921 ttcaatgtct tgtaatttca aggccttgcg tttaatcaag tccaagaggc gacctggagt
    19981 tcccaccacg atatgggcac cagatttaag agccttaatt tgtttttcaa tgcttgatcc
    20041 gccatatact gaacggactt tgactccctt actacgacca aagcggaaga gttcttcttg
    20101 actttggaca gctagttcac gagttggagc gatgaccaag gcttgaatag tcgcttcttc
    20161 tatacggatt ttttcaaggg taggcaagcc aaaggctgca gtttttcctg taccagtctg
    20221 agcttgaccg ataacatcct tgccttcaag ggccaaagga atagtttgtt cttggatagg
    20281 actagcttct acaaaaccag ctttttcaat ttctgctagc aaatcagcag acaagtttaa
    20341 ttcattaaat ttcacgttat tcttctttct aaaggtggtg cgaagccacc ctatagggct
    20401 tagtttatac ttttcttttt atgacgtatt ttcatataac tagatataaa atcgtgttgc
    20461 ttcttttcca caaaagaaaa gtactgtttt ctttgcaacc tatctagtat aacacaagac
    20521 cagagcaaaa gatagcccca tttctacaga aaatcatgta agcggttttt gactttcttt
    20581 tttgattgaa cgacctagat aataagacaa agccaaggcg atactgtata aaatgagaaa
    20641 aacgaacaag gtttgtgtgt acgaatgagc cattttataa gtctctgcta ataaaatagg
    20701 tcccgctaaa ccagccattg cccaagctgt taaaatataa ccatgcagag cggccaattc
    20761 cttggttcca aaaatatcac tgagataagc tggaatcaaa gaaaaaccag ctccatagca
    20821 agtcatcaaa atagacatag caactacaaa taaaacggaa tctgtaaaga gccaaagtga
    20881 gagagaaaag aaaagattga caagcagtaa tatactaaag gttagagggc gaccgatata
    20941 gtcagacaaa ctcgcccaga gcaagcgacc aaatccattg aaaatcccca aaacacccac
    21001 cattactgct gcatgacttg tagacaagcc agccatctcc tgtgccattg gcgatgccgc
    21061 tgaaattaag cctaaaccac aagctatgtt gataaagaaa ataatccaaa gcatataaaa
    21121 ccgattgctt tttagagcct gatttgcagc cattccttgc gtcaaagagg ctgttttttc
    21181 tttccctgaa gaagataaaa ttgcaagctc ttgctcattt ggacgcttaa tgaattgtga
    21241 agctaggagc atgataataa agtaacttgc tcctaaaata taaaaagttt ctacaatccc
    21301 tacccctgcg atgaggtgtt gcgctatggg actagtcaat aaagaagcaa aaccaaaccc
    21361 cataatcgct aaacctgttg cgagaccacg tttatcagga aaccatttta taatcgttga
    21421 cacaggggta atatagcctg ctcccaaacc aagcccacct aaaatgccat aagctagata
    21481 caacaaccac agttcctgac ggtctattgc aaatcctgtt aagatatttc cacctgcgta
    21541 tagaaaagca gatagactcc ccatgacttt cggaccaaat ttttctacca aacgccccat
    21601 aaatgcagcc gataagccca aacaaaagat tgctagacta aaggcgaagg caacagaagc
    21661 ctgatcccat cccgtttttt caataatagg gttacgataa acactccaag cataagtcga
    21721 acccagcatt aagtgtaaaa tgaccccagc aaaggcaata atataacgat tcgatttcat
    21781 aaaacctcct attttcgaac atttattctt tcttatataa aaatagatag gattttagtt
    21841 tatcaagctt gtcaaaaaat tactagtttt ttgtctggaa agcgttttta tctttttctg
    21901 tcttttccgt agcagaattg tgcaaaagtt tttttcttgt gctagaatga aattcgaata
    21961 ggaggaagat aaatgttaac atacgattta attgttattg gatttggtaa agctggtaaa
    22021 acactagcag gtaaattggc ttcagctggc aaaaaagttg ccctcgttga acgcagcaag
    22081 gctatgtacg gtggaacttg tatcaacatt ggttgtatcc caactaaaac cttgctagtt
    22141 gctgctgaaa aggacttgtc ttttgaagaa gtgattgcta ctaaaaacac gatcactggt
    22201 cgcctcaacg gtaaaaacta tgcgactgtt gctggtacag gcgtagatat ctttgatgcg
    22261 gaagctcact tcctttcaaa taaagtcatc gaaatccaag ctggtgatga aaagaaagaa
    22321 ctgactgctg aaacaatcgt catcaacact ggtgctgttt caaacgtctt gccaattcct
    22381 ggacttgcta caagcaaaaa catctttgac tcaacaggta tccaaagctt ggacaaatta
    22441 cctgaaaaac ttggaatcct tggtggcgga aatatcggtc ttgaatttgc cggcctttac
    22501 aacaaacttg gaagcaaggt cacagtccta gatgccttgg atacattcct acctcgtgca
    22561 gaaccttcca tcgcagctct tgctaaacaa tacatggaag aagatggcat tgaattgctt
    22621 caaaatatcc atactactga aatcaaaaac gatggtgacc aagtgcttgt cgtaactgaa
    22681 gacgaaactt accgtttcga cgcccttctc tacgcaactg gacgcaaacc aaatgtagaa
    22741 ccacttcaac ttgaaaatac agatattgaa ctaactgaac gtggtgctat taaagtagac
    22801 aaacactgtc aaacaaacgt tcctggtgtc tttgcagttg gagatgtcaa cggtggcctt
    22861 caatttactt acatttcact tgatgacttc cgtgttgttt acagctacct tgctggagat
    22921 ggcagctaca cacttgagga ccgtctcaat gtgccaaata ctatgttcat cacacctgca
    22981 ctttcacaag ttggtttgac tgaaagccaa gcagctgatt tgaaacttcc atacgcagtg
    23041 aaagaaatcc ctgttgcagc catgcctcgt ggtcacgtaa atggagacct tcgcggagct
    23101 ttcaaagctg ttgttaatac tgaaacaaaa gaaattcttg gtgcaagcat cttctcagaa
    23161 ggttctcaag aaatcatcaa catcattact gttgctatgg acaacaagat tccttacact
    23221 tacttcacaa aacaaatctt cactcaccca accttggctg agaacttgaa tgacttgttt
    23281 gcgatttaag ttgaaatctc atcttaactg acagccctct ttgggctgtt tttacttcta
    23341 cgaaacacca aatctgtctt ttccctcttt tgtgatataa tagaaacatg aacttaaaaa
    23401 ctactttggg ccttcttgct gggcgttctt cccacttcgt tttaagccgt cttggacgtg
    23461 gaagtacgct cccagggaaa gtcgcccttc aatttgataa agatatttta caaagcctag
    23521 ctaagaacta cgagattgtc gttgtcactg gaacaaatgg aaaaaccctg acaactgccc
    23581 tcactgtcgg cattttaaaa gaggtttatg gtcaagttct aaccaaccca agcggtgcca
    23641 acatgattac agggattgca acaaccttcc taacagccaa atcttcaaaa actgggaaaa
    23701 atattgccgt cctcgaaatt gacgaagcca gtctatctcg tatctgtgac tatatccagc
    23761 ctagtctttt tgtcattact aatatcttcc gtgaccagat ggaccgtttc ggtgaaatct
    23821 atactaccta taacatgata ttggatgcca ttcgaaaagt tccaactgct actgttctcc
    23881 ttaacggaga cagtccactt ttctacaagc caactattcc aaaccctata gagtattttg
    23941 gttttgactt ggaaaaagga ccagcccaac ttgctcacta caataccgaa gggattctct
    24001 gtcctgactg ccaaggcatc ctcaaatatg agcataatac ctatgcaaac ttgggtgcct
    24061 atatctgtga aggttgtgga tgtaaacgtc ctgatctcga ctatcgtttg acaaaactgg
    24121 ttgagttgac caacaatcgc tctcgctttg tcatagacgg ccaagaatac ggtatccaaa
    24181 tcggcgggct ctataatatc tataacgccc tagctgctgt ggccatcgcc cgtttcctcg
    24241 gcgcagattc ccaactcatc aaacagggat ttgacaagag ccgtgctgtc tttggacgcc
    24301 aagaaacctt tcatatcggt gacaaggaat gtacccttgt cttgattaaa aatccagtcg
    24361 gtgcaaccca agctatcgaa atgatcaaac tagcacctta tccatttagc ctatctgtcc
    24421 tccttaatgc caactatgca gatggaattg acactagctg gatctgggat gcagactttg
    24481 agcaaatcac tgacatggac attcctgaaa tcaacgctgg cggtgttcgt cattctgaaa
    24541 tcgctcgtcg cctccgagtg actggctatc cagctgagaa aatcactgaa acgagtaatc
    24601 tggagcaagt tctcaagacc attgagaatc aagactgcaa gcatgcctat attctggcaa
    24661 cttatactgc catgctggaa tttcgtgaac tgctggctag tcgtcagatt gttagaaagg
    24721 agatgaacta atggtttata cttcactttc ctcaaaagat agcaattacc cctatcagct
    24781 caacattgcc cacctctacg gaaatctcat gaatacctac ggggacaatg gaaacatcct
    24841 catgctcaag tatgtggctg aaaaactggg agcccatgtg accgttgaca tcgtttctct
    24901 ccatgatgac tttgatgaaa atcactacga catcgccttt ttcggtggtg gtcaagactt
    24961 tgaacaaagt atcattgcag acgacctacc tgctaaaaaa gagagcattg acaactacat
    25021 ccaaaacgac ggtgtagttc tggctatctg cggtggtttc caactattgg gtcaatatta
    25081 tgttgaagct tcaggaaaac gtatcgaagg gctaggggtc atgggacact acacgctcaa
    25141 ccagaccaat aaccgtttta tcggtgacat caagattcac aatgaagatt tcgatgaaac
    25201 ctactatgga tttgaaaatc accaaggtcg taccttcctc tctgatgacc aaaaaccgct
    25261 gggacaggtt gtctatggaa atggaaacaa cgaagaaaag gtcggtgaag gggttcatta
    25321 taagaatgtc tttggttcct acttccacgg gcctatcctc tctcgtaatg ccaatctggc
    25381 ttatcgccta gttactactg ccctcaagaa gaaatatggt caggacatcc aactccctgc
    25441 ctatgaggac attctcagcc aagaaatcgc tgaagagtac agtgacgtca aaagcaaggc
    25501 tgacttttct taaacaaagg aaaatgatat caaagaactc cgttatcttg tcggagtttt
    25561 ttgtcttttc ttttaccctt ctcccttgca ttttctctca ttttttgcca aaatagaggg
    25621 gtagaaagaa ggtagcatat gtctaaatta caacaaatcc taacatatct tgaatcagaa
    25681 aaactagacg tcgctgtcgt atctgacccc gtcacaatca attacctcac tggtttttac
    25741 agtgatcccc atgaacgcca aatgttcctc tttgtcctag cggatcagga acctctcctc
    25801 tttgtcccag ctcttgaagt agaacgtgca agtagcaccg tttccttccc agtagtgggc
    25861 tatgtcgatt ctgaaaatcc atggcaaaaa atcaaacatg ctcttccaca acttgacttc
    25921 aaacgtgtcg ctgttgagtt tgacaatctc atcttgacca aataccatgg tttgaaaaca
    25981 gtttttgaga ctgctgagtt tgacaacctc actcctcgta tccaacgcat gcgcctcatc
    26041 aaatcagctg atgaagtgca aaaaatgatg gttgcaggtc tttatgctga caaggctgtt
    26101 catgttggtt ttgacaatat ttctcttgat aagactgaga cagatatcat cgcacaaatc
    26161 gactttgcca tgaaacgtga aggttatgaa atgagctttg ataccatggt cttgactggt
    26221 gataatgctg cgaatccaca cggcattcca gcagctaata aggttgaaaa tgatgctctt
    26281 ctcctctttg acctgggtgt tctggtcaat ggctatgcgt cagatatgac tcgtacagtc
    26341 gctgtcggca aaccagacca attcaagaaa gatatttaca acttgactct tgaagcccaa
    26401 caagctgctc ttgactttat caagccaggt gtgactgctc atgaagtgga ctgcgctgcc
    26461 cgtgaggtca tcgaaaaagc tggttatggt gagtacttca accaccgtct cgggcatggt
    26521 atcggtatgg atgtccatga attcccatct atcatggaag gaaacgacat ggtcatcgaa
    26581 gaaggcatgt gcttctctgt tgaaccaggt atctatatcc ctggtaaagt cggtgttcgt
    26641 attgaagact gcggtgttgt taccaaggat ggcttcgacc tctttacaag caccagcaaa
    26701 gatttgcttt attttgatta aactatatag cccctatgct ttcctttcaa aatatctagg
    26761 ggctatttta ttgtcatttt tctgctatta tgctaaagaa attggctgca ataatctaac
    26821 cctaagtgtc tggaatgata acgagggtgc tctccgcttt tatcaaagac aagggatgaa
    26881 accccaagaa acaacaatgg aaatgataat tgattaagaa gtcatctatc aaaagatgtt
    26941 agaaaaagtt caatttcact agaaaatgag gaaaatcttc ccacaataaa acgcatagta
    27001 tcaagtgtta aaatgtactg ccccccaaaa gttagacaaa taatttatcc gaaggattta
    27061 gttctgtatt gcacagggct aagtcctttt agttttacct taattctctt attgttgtag
    27121 taatcaatat agtctacaat ggcttgttcc aattgcttaa gcgactgaaa tgacttctca
    27181 taaccataaa acatctccga tttcagaatg ccaaagaagg actccatcat accgttgtct
    27241 gggctgttac ctttacgtga catagatgct tgaattccct tactctctag gaaccgatga
    27301 taagaatcgt gttgatattg ccagccttgg tcactatgga gaatcgtatt ctcgtagtgc
    27361 ttctct
//
LOCUS       DAVLSL010000012        27284 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLSL010000012 DAVLSL010000000
VERSION     DAVLSL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.012"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(377..469)
                     /locus_tag="VXN30_000407"
     CDS             complement(377..469)
                     /locus_tag="VXN30_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1357080.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            630..1601
                     /gene="ruvB"
                     /locus_tag="VXN30_000408"
     CDS             630..1601
                     /gene="ruvB"
                     /locus_tag="VXN30_000408"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1357081.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1582..2154
                     /locus_tag="VXN30_000409"
     CDS             1582..2154
                     /locus_tag="VXN30_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1357082.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2385..3143
                     /locus_tag="VXN30_000410"
     CDS             2385..3143
                     /locus_tag="VXN30_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1357083.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALIKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3152..3955
                     /locus_tag="VXN30_000411"
     CDS             3152..3955
                     /locus_tag="VXN30_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1357084.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            3977..5236
                     /gene="rseP"
                     /locus_tag="VXN30_000412"
     CDS             3977..5236
                     /gene="rseP"
                     /locus_tag="VXN30_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1357085.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5249..7102
                     /locus_tag="VXN30_000413"
     CDS             5249..7102
                     /locus_tag="VXN30_000413"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1357086.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7202..8581
                     /gene="bglA"
                     /locus_tag="VXN30_000414"
     CDS             7202..8581
                     /gene="bglA"
                     /locus_tag="VXN30_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1357087.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            8773..10581
                     /gene="glmS"
                     /locus_tag="VXN30_000415"
     CDS             8773..10581
                     /gene="glmS"
                     /locus_tag="VXN30_000415"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1357088.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            10700..11749
                     /locus_tag="VXN30_000416"
     CDS             10700..11749
                     /locus_tag="VXN30_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1357089.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(11849..15655)
                     /gene="spuA"
                     /locus_tag="VXN30_000417"
     CDS             complement(11849..15655)
                     /gene="spuA"
                     /locus_tag="VXN30_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1357090.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEAQE
                     ASVKEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLL
                     KNKKEN"
     gene            16072..16485
                     /gene="rpsL"
                     /locus_tag="VXN30_000418"
     CDS             16072..16485
                     /gene="rpsL"
                     /locus_tag="VXN30_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1357091.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16505..16975
                     /gene="rpsG"
                     /locus_tag="VXN30_000419"
     CDS             16505..16975
                     /gene="rpsG"
                     /locus_tag="VXN30_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1357092.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17400..19481
                     /gene="fusA"
                     /locus_tag="VXN30_000420"
     CDS             17400..19481
                     /gene="fusA"
                     /locus_tag="VXN30_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1357093.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19585..23976
                     /locus_tag="VXN30_000421"
     CDS             19585..23976
                     /locus_tag="VXN30_000421"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1357094.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKFLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24078..24341
                     /locus_tag="VXN30_000422"
     CDS             24078..24341
                     /locus_tag="VXN30_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1357095.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24334..24612
                     /locus_tag="VXN30_000423"
     CDS             24334..24612
                     /locus_tag="VXN30_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1357096.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            24633..24785
                     /locus_tag="VXN30_000424"
     CDS             24633..24785
                     /locus_tag="VXN30_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357097.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            24807..26048
                     /locus_tag="VXN30_000425"
     CDS             24807..26048
                     /locus_tag="VXN30_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1357098.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26058..26288
                     /locus_tag="VXN30_000426"
     CDS             26058..26288
                     /locus_tag="VXN30_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1357099.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            26556..27278
                     /locus_tag="VXN30_000427"
     CDS             26556..27278
                     /locus_tag="VXN30_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1357100.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT         7686 a         5189 c         6468 g         7941 t
ORIGIN      
        1 agcgaatata actaagaaat aggtctgtgg tcgcttcagc tagttcaaac tcaatttgat
       61 tgtaagtgac tgttaattga agaactggaa ctactgtaat aatgattttg cttgtctagt
      121 agtggcgtat taaatgagaa gtaacatgta agattgtctc ttcgtttacg aaatcaaaat
      181 ttctatgaaa ttgatagaat ccttaacgtt ctcttaatct taaccgtctt gtcgtacagc
      241 ggttgaagaa aatgtaaacc tttgacaaaa ttcgtgaact atgggataat aaaaaggaat
      301 tgagtactag ttctatatca taggctagaa aagaccccaa gctcacgacc aaataagctt
      361 gaggtctttt ttgacactat ttgtccttgt ttagccattt atcgactata cgaagaacga
      421 caccgaccac aattggtccg atgatagttt tgagtactaa ttccatcatg ggctatctca
      481 cctcctttcg taggcggtgt agcagtgtcg tagaatatta taccacataa gtgctaaact
      541 taggagtcac ccaatcaggt ggcttttttg tttgtggctt ggttttttga tataatagag
      601 ccatgagtag aattttagat aatgagataa tgggggatga ggagttagta gaacgcacgc
      661 tccgtcctca gtatttacgt gaatatatcg gacaggataa ggtcaaggac cagctacaaa
      721 tctttattga agctgccaaa atgcgggatg aagcgctgga tcatgtgctc ttatttgggc
      781 ctccaggtct cgggaaaacg accatggcct ttgttattgc caacgaactg ggagtcaatc
      841 ttaagcagac gtcgggtcca gtcattgaaa aagccggaga tctggtagct attttgaatg
      901 agttagagcc tggggatgta ctttttattg atgagatcca tcgtttgcca atgtcagtgg
      961 aagaggtgct ttatagtgct atggaggact tctacatcga tattatgatt ggggctggtg
     1021 agggtagtcg tagtgttcat ttggagttac caccttttac cttgattggt gcgacgactc
     1081 gggctggtat gctctccaat ccgctacggg cacgttttgg gattacaggc catatggagt
     1141 attatgccca tgctggcttg acagaaattg tcgagcggac ggcagatatt tttgagatgg
     1201 aaatcactca tgaggcagca tctgagttgg ccctacgtag tcgtgggacc cctcgtattg
     1261 ccaatcgtct cctcaagcgc gtgcgcgatt ttgcccagat aatggggaat ggggtaattg
     1321 atgatattat taccgataag gctttgacta tgctggatgt tgaccatgaa ggtttggact
     1381 atgtggatca aaaaatcctt cgtaccatga ttgagatgta cagtggagga cctgttggtc
     1441 taggaactct ttctgtgaat atcgccgaag agcgtgagac agttgaagat atgtatgagc
     1501 cttacttgat tcaaaaaggt tttatcatgc ggacacggtc tggacgggtg gcgactgcta
     1561 aggcatatga gcacttaggt tatgaataca gtgaaaaata agcaagaaat cttagaggct
     1621 tttagagaaa atccagatat gatggccatt ctgacgatca tccgagacct tggtctgaaa
     1681 gactcgtggt tggcagcagg ttctgtcaga aatttcattt ggaatctttt gtcagacaaa
     1741 tccccttttg atcatgaaac agatatagat gtgattttct ttgatccaga tttttcttat
     1801 gaggaaacct tattactgga gaaaaagctg cgagaggatt ttcctcagta ccagtgggaa
     1861 ttgaaaaatc aggtctatat gcatcagcac agtcctcaca ctgcttccta taccagttct
     1921 cgtgatgcta tgagtaagta tccagaacgg tgtacgacag ttggactgcg cttgaatgaa
     1981 gaatcagatt ttgaactcta tgcaccttat ggtttggagg atattttgaa ttttcaagtt
     2041 cgtccaactc ctcatttctt agaaaatgaa gaccgaatgg aactctatca aacacgttta
     2101 tccaagaaaa attggcagga gaaatggaaa aatttgattt ttaaaaatac ttaaggaaac
     2161 tttaagctag ggagtgtaca ctaagttcat aagttaagaa gaccttaact taaactccta
     2221 aaacttcttc ataataatct ccctataaaa ataaagtcgc ccaatcaggc ggcttatttt
     2281 tttgaaaaat gggcttggtg cctgagaata aatagcttag tgatagaaga aaatggggaa
     2341 atatggtata atgaaacgat agatttttga ataggaataa gatcatgttt ggatttttta
     2401 agaaagataa ggctgtggaa gtagaggttc cgacacaggt tcctgctcat atcggcatca
     2461 tcatggatgg caatggccgt tgggctaaaa aacgtatgca accgcgagtt tttggacata
     2521 aggcgggcat ggaagcattg caaaccgtga ccaaggcagc caacaaactg ggcgtcaagg
     2581 ttattacggt ctatgctttt tctacggaaa actggacccg tccagatcag gaagtcaagt
     2641 ttatcatgaa cttgccagta gagttttatg ataattatgt cccggaacta catgcgaata
     2701 atgttaagat tcaaatgatt ggggagacag accgcctgcc taagcaaacc tttgaagctt
     2761 taatcaaggc tgaggaattg actaagaaca acacaggatt gattcttaat tttgctctta
     2821 actatggtgg acgtgctgag attacacagg cgcttaagtt gatttcccag gatgttttag
     2881 atgccaaaat caacccaggt gacatcacag aggaattgat tggcaactat ctctttaccc
     2941 agcatttgcc taaggactta cgagacccag acttgattat ccgtactagt ggagaattgc
     3001 gtttgagcaa tttccttcca tggcagggag cctatagtga gctttatttt acggacacct
     3061 tatggcctga ttttgacgaa gcagccttgc aggaagctat tcttgcctat aatcgtcgcc
     3121 atcgccgatt tggaggagtt taggaggaaa tatgacccag gatttacaga aaagaacctt
     3181 gtttgcaggg attgccctgg ctattttcct accaatttta atgattgggg gcctcttgct
     3241 tcagatagca atcggaatca tagccatgct agccatgcat gaacttttga agataagagg
     3301 tctagcgacc atgacgatgg agggcctctt gactctcttt gcaacctttg cattaaccat
     3361 tcccttggag aattacctga cttttttgcc agttgatggg aatgtggttg cctatagtgt
     3421 tttgatttca atcatgttag gaacgaccgt ttttagcaag tcttatacga ttgaggatgc
     3481 ggttttccct cttgctatga gcttctacgt gggctttgga tttaatgctt tactagatgc
     3541 tcgtgttgca ggtttggaca aggctctctt agccttgtgt atcgtctggg cgacagacag
     3601 tggtgcctat cttgttggga tgaactatgg gaaacgaaag ttagcaccaa gggtatcgcc
     3661 taataaaacc cttgagggtg ccttgggtgg tattttagga gcaattttag taaccattat
     3721 ctttatgata gttgacagta cagttgctct tccatatgga atttacaaga tgtcagtctt
     3781 tgctattttc tttagcattg ctggacaatt tggtgattta ctagaaagtt cgatcaaacg
     3841 tcattttggt gttaaggatt ctgggaaatt tatccctgga catggtggtg ttttggatcg
     3901 tttcgatagt atgttgcttg tatttccaat catgcactta tttggactct tttaatcaaa
     3961 agacggagga aacgctatgc tcggaatttt aacctttatt ctggttttcg ggattattgt
     4021 agtggtgcac gagttcgggc acttctactt tgccaagaaa tcagggattt tagtacgtga
     4081 atttgccatc ggtatgggac ctaaaatttt tgctcacatt ggcaaggatg gaacggccta
     4141 taccattcga atcttgcctc tgggtggcta tgtccgcatg gccggttggg gtgatgatac
     4201 aactgaaatc aagacaggaa cgcctgttag tttgacactt gctgatgatg gtaaggttaa
     4261 acgcatcaat ctctcaggta aaaaattgga tcaaacagcc ctccctatgc aggtgaccca
     4321 gtttgatttt gaagacaagc tctttatcaa aggattggtt ctggaagaag aaaaaacatt
     4381 tgcagtggat cacgatgcaa cggttgtgga agcagatggt actgaggttc ggattgcacc
     4441 tttagatgtt caatatcaaa atgcgactat ctggggcaaa ctgattacca attttgcagg
     4501 tcctatgaac aattttatct taggtgtcgt tgttttttgg gttttaatct ttatgcaggg
     4561 tggtgtcaga gatgttgata ccaatcagtt ccatatcatg ccccaaggtg ccttggccaa
     4621 ggtaggagta ccagaaacgg cacaaattac caagattggc tcacatgagg ttagcaactg
     4681 ggaaagcttg atccaagctg tggaaacaga aaccaaagat aagacggcac cgactttgga
     4741 tgtgactatt tctgaaaagg ggagtgacaa acaagtcact gttacacccg aagatagtca
     4801 aggtcgttac cttctaggtg ttcaaccggg ggttaagtca gattttctat ccatgtttgt
     4861 aggtggtttt acaactgctg ctgactcagc tctccgaatt ctctcagctc tgaaaaatct
     4921 gattttccaa ccggatttga acaagttggg tggacctgtt gctatcttta aggcaagtag
     4981 tgatgctgct aaaaatggaa ttgagaatat cttgtacttc ttggcaatga tttccatcaa
     5041 tattgggatt tttaatctta ttccgattcc agccttggat ggtggtaaga ttgtgctcaa
     5101 tatcctagaa gccatccgcc acaaaccatt gaaacaagaa attgaaacct atgtcacctt
     5161 ggccggagtg gtcatcatgg ttgtcttgat gattgctgtg acttggaatg acattatgcg
     5221 actctttttt agataatcga ggaatattat gaaacaaagt aaaatgccta tcccaacgct
     5281 tcgcgaaatg ccaagcgatg ctcaagttat cagccatgct cttatgttgc gtgctggtta
     5341 tgttcgccaa gtttcagcag gtgtttattc ttatctacca cttgccaacc gtgtgattga
     5401 aaaagctaaa aacatcatgc gccaagaatt cgaaaagatt ggtgctgttg agatgttggc
     5461 tccaaccctt cttagtgcag aattgtggcg tgaatcaggt cgttacgaaa cctatggtga
     5521 agacctttac aaactaaaaa accgtgaaaa atcagacttt atcttaggtc caactcacga
     5581 agaaaccttt acagctattg tccgtgattc tgttaaatct tacaagcaat tgccactcaa
     5641 cctttatcaa attcagccca agtatcgtga tgaaaaacgc ccacgtaatg gacttcttcg
     5701 tacacgtgag tttatcatga aggatgctta tagtttccac gctaactatg atagtttgga
     5761 tagtgtttat gatgagtaca aagcagccta tgagcgtatt ttcactcgta gtggtttaga
     5821 cttcaaggct attattggtg acggtggagc catgggtggt aaggatagcc aagaatttat
     5881 ggccattaca tctgctcgta cagaccttga ccgctgggtt gtcttggaca agtcagttgc
     5941 ctcatttgac gaaattcctg cagaagtgca agaagaaatc aaggcagaat tgctcaaatg
     6001 gatagtctct ggtgaagata ccattgctta ctcaagtgag tctagctatg cagctaactt
     6061 agaaatggca acaaacgagt acaaaccaag caaccgtgtt gtcgctgaag aagaagttac
     6121 tcgtgttgca acgccagatg ttaaatcaat tgatgaagtt gcagccttcc tcaatgttcc
     6181 agaagaacaa acgattaaaa ccctcttcta cattgcagat ggtgagcttg ttgcagccct
     6241 tctagttgga aatgaccaac tcaacgaagt caagttgaaa aatcacttgg gagcagattt
     6301 ctttgacgtt gctagcgaag aagaagtggc gaatgttgtt caagcaggat ttggttcact
     6361 tggaccagtt ggtttgccag agaatattaa aattattgca gatcgtaagg tgcaagatgt
     6421 tcgcaatgca gttgtcggtg ctaacgaaga tggctaccac ttgactggtg tgaacccagg
     6481 ccgtgatttt actgcagaat atgtggatat ccgtgaagtt cgtgagggtg aaatttcccc
     6541 agatggacaa ggtgtcctta actttgcgcg tggtattgag atcggtcata ttttcaaact
     6601 cggaactcgc tactcagcaa gcatgggagc agatgtcttg gatgaaaatg gtcgtgctgt
     6661 gccaatcatc atgggatgtt acggtatcgg tgtcagccgt cttctttcag cagtgatgga
     6721 gcaacacgct cgcctctttg ttaacaaaac gccaaaaggt gaataccgtt acgcttgggg
     6781 aatcaatttc cctaaagaat tggcaccatt tgatgtgcat ttgattactg tcaatgtcaa
     6841 ggatgaagaa gcgcaagcct tgacagaaaa acttgaagca agcttgatgg gagctggtta
     6901 tgaagtcttg acagatgacc gtaacgaacg tgtcggtgtt aaatttagcg atagtgactt
     6961 gattggtctt ccaatccgta tcactgttgg taaaaaagcg gccgatggca ttgtagaagt
     7021 taagatcaag gcaactggtg acaccattga ggttcatgca gataacgtgc ttgaaacgct
     7081 tgaaatccta agcaagaaat aaaaactata atcagaagaa aaacaaggaa aaaatgtaac
     7141 tagtttttac cttgtttttt ctgtataatg ggagaaatga gtagataaag aggtaaatgt
     7201 tatgctaaga tttccaaagg attttgtctg gggatcctct acttctggac cgcagacaga
     7261 aggacgtgta gctggtgacg gtaagggaga caatctctgg gattactggt tccaagtgga
     7321 gccaaatcgt tactataatg ggattggttc agataagaca tcgacttttt atgaaaattg
     7381 ggagcgggat attgagcttt tgttagagac tggtcacaca gtctttcgga cttctattca
     7441 gtggtcacgg atttttccac aaggctgtgg aaaagtcaac cctcaaggtg tggattttta
     7501 tcgtaaggtc tttgaggcta ttaaggctaa aggaattcgt ctgttagtca atctctatca
     7561 ttttgattta ccttttgccc ttcaagagga tggtgatggt tgggaaaata aggcgacagt
     7621 ctcagcctat gaagactatg ctcgtttttg ttttgagact tatggagatt tagtggatca
     7681 atggattacc tttaacgagc ccatcgttcc tgtagaattt ggttattttt atgatgccca
     7741 ttatccacat aaggtggatg cagaggcagc tgttaaggta gcctatcata cacaattggc
     7801 cagcagccgg gctgttaagg cttgccatga acttttgcct gattccaaga ttgggattgt
     7861 cctcaacttg acaccggctt atccacgtag ccagcatcct gctgatgtca aggcagctcg
     7921 tattgcggcc ctttttcagg cccaatcttt cttagatcca tctgtcttgg ggacttatcc
     7981 acaggagttg gtagaaatct tgcatgaaca cggtctttta cctgatgcta cagaggaaga
     8041 gttggaactc attcgtgata atacggtgga cttccttggt gtgaactact atcaaccttt
     8101 gcgtgttatg gctcctcgat ttgctaagca tccagatagt ccactcttac cagaacattt
     8161 ttacgagcct tatgtgatgc ctggacgtaa aatcaattct caccgtggtt gggagattta
     8221 tgagcaaggg atttatgaca tcgcccaaaa tatcaaggaa aattatggca atattgagtg
     8281 gatgttgact gagaatggta tgggtgttga aggggaagaa aaattccgtc aagatggaat
     8341 gattcaagat gattaccgta ttgactttgt aaaaggtcat cttcgtgaac ttcaccgtgc
     8401 cattgaagat ggtgccaact gtaagggcta cttgatttgg acctttattg actgctggtc
     8461 atggctcaac agctataaaa atcgctatgg tttggtcgaa ttagacttgg aaacgcaaga
     8521 acgtcgtctg aagaaatcag gccactggtt caaagaatta agcgataata atggatttta
     8581 aaaaggactc tgactgatta tcccaatcgg tcagagtttt ttgcttacag gagcttaatt
     8641 tgaactatac caatttttac tcttgacaag tgaaagaaac aagtatatac tgtttttgct
     8701 gattctataa aagatgaatt agactatacc aattttaagg agaaagcaca gcttgcctgt
     8761 gtcgtatata ctatgtgtgg aattgttggt gttgttggaa acacaaatgc aactgatatt
     8821 ttgattcaag ggcttgaaaa gcttgaatac cgtggctatg attctgcggg aatttttgtc
     8881 ctagatggtg ctgataacca tttggtgaag gctgttggtc gtatcgcaga attgtctgcc
     8941 aagacagctg gtgttgaggg aacaactggt atcggacata ctcgttgggc aactcatggg
     9001 aaaccaacgg aagacaatgc tcacccacac cgctctgaga cagaacgttt tgtcttggtt
     9061 cacaatgggg tgattgaaaa ctaccttgaa attaaagaag aataccttgc tgggcaccac
     9121 ttcaaagggc aaacagatac ggaaatcgcc gtacatttga ttggaaaatt tgcggaagaa
     9181 gacggtctct cagttcttga agcctttaaa aaagctcttc atattatccg tggttcatat
     9241 gcctttgcct tgattgactc tgaaaatcca gatgtcatct atgtagcgaa aaacaaatct
     9301 ccacttttga ttggtcttgg ggaaggctac aatatggtct gctcagatgc tatggctatg
     9361 attcgtgaaa ccaaccaata catggaaatt catgaccaag agttggtaat cgtcaaggct
     9421 gatagcgtgg aagttcaaga ctatgatggt aacagtcgtg aacgtgctag ctatactgcg
     9481 gaacttgact tgtcagatat cggtaaggga acttatcctt actacatgct taaggaaatt
     9541 gatgagcaac caactgttat gcgtaaactc attcaagcct acacggatga tgctggtcaa
     9601 gtagtgattg atcctgctat cattaaggct gttcaagacg cagaccgcat ctacatcctt
     9661 gcagctggaa catcttacca tgcaggattt gcttctaaga aaatgttgga agaattgaca
     9721 gatacaccag ttgaacttgg catctcatct gagtggggct acggtatgcc acttctcagc
     9781 aagaaaccac tcttcatctt tatcagccaa tctggtgaaa cagcggatag tcgtcaagtt
     9841 ttggtcaagg ctaatgaaat gggaattcca agcttaacag tgacaaacgt tccaggttca
     9901 accctctcac gtgaagccaa ctataccatg ctccttcacg caggtcctga aattgccgtg
     9961 gcatcaacta aagcctatac agcgcaaatc gcagcccttg ccttccttgc aaaagcagtc
    10021 ggagaagcaa atggtaatgc taaagcgcaa gcctttgacc tggttcatga attgtcaatc
    10081 gtagctcagt ctatcgaatc aactctttca gagaaagaaa ccattgaagc caaggttcgt
    10141 gaacttcttg aaacaactcg taacgccttt tacatcggac gtggtcaaga ttactacgta
    10201 gccatggaag caagtctcaa actcaaagag atttcttata tccagtgtga aggttttgcg
    10261 gcaggagaac tcaagcacgg aaccattgcc ttgattgaag aaggaacacc tgtcttggct
    10321 ctcttgtcag atccagtcct tgccaaccac actcgtggaa atatccaaga ggtggcagcc
    10381 cgtggtgcta aggtcctcac tatcgcagaa gaaaatgttg ctaaagatac cgacgatatc
    10441 gtccttacga ctgtacaccc ttacctctca ccaatttcaa tggtcgtacc gacgcaattg
    10501 gttgcttact ttgcaaccct ccaccgtggc ctcgatgtgg ataaaccacg taaccttgct
    10561 aaatcagtaa cggtagaata agctaaaaaa gtctagttat ctaggctttt tctaatgagc
    10621 aaattggttg cttgtactca agattcgtgt tagaataatt tttcaaattg aaggacagag
    10681 atagacaagg agaatcacaa tggtagaatt gggaatttca acatttgggg aaataacgga
    10741 gcttgaaggg actggacaaa cttacagtca tgctgaacgc attcgccagt tggtggcaga
    10801 gattgagctg gctgacaagg ttggtttgga cgtatatggg attggcgagc accatcgggc
    10861 ggattttgca gtatcagccc cagagattgt cctggccgct ggggcagtca ataccaagaa
    10921 aatccgtttg accagtgcag tcagcattct gtcaagcatg gatccgattc gtttgttcca
    10981 acagtatgcc actatcgatg ctttgtcaaa tggacgatcg gagattatgg ctggaagggg
    11041 ctctttcacg gaatctttcc ctctgtttgg ttatgatttg aaagactacg actctctttt
    11101 tgatgagaaa ttagacttgc ttcagttagt caatgaaaag accaagttag actggcaagg
    11161 tcgattgact caaacgattg ctggtaaaga agtttatcct cgtccagttc aggacaaatt
    11221 gcccttgtgg atagctacag gtggtcatgt cgaatcaaca gtgaagattg ctcaggctgg
    11281 tctaccgatt gtatatgcta ttattggtgg taatccacgt tattttaaaa agttgattca
    11341 ggcttatcgt gagattggaa gcgaagcggg ccatgctgac aaagacttga aagtgggagc
    11401 ccattcttgg gggtggattg cggaagatgg tgagcaggcg gtgaaagatt atttccatcc
    11461 gactaagcaa gtggtggatg ctatttccaa agaccgtccg cactggcagg aattgcgtta
    11521 tgagcaatat ttggagcagg tagggccaaa tggcgccatg tttgttggca atccagatca
    11581 ggtggccgaa aaattgattc gcatgataga ggatttagga ttggaccgct tcatgcttca
    11641 tctaccgctt ggttctatgc ctcatgacca ggttctgaga gctattgaac tcttcggaac
    11701 acaagttgca cccaaagtac gagcttattt tgctatgaaa gaggcttaat aaaaaatcct
    11761 aatcaagttt attaggacaa caaatattgt acaaaaatta atcccccgag ctataatgct
    11821 tgggggattt ttctatagga gggctagttt agttctcttt tttatttttt agtaagaaac
    11881 cgagaccaag gagcgcaagg aggctgattc ctgcaaatag gagtttgttt tcgtttttga
    11941 ttcctgtatt tggaagttca gcttgtttat ctggggttgc aggtatattt tccttgctag
    12001 agttttctac cgattcgttt cgaaccgctt cttttacaga tgcttcttgt gcttcttgtg
    12061 cttcttgtgc tggttgttca gcttgtgaat cagctgtagc ttggctaggt ttattttccg
    12121 catcagctac tttggcatct ggtttagtag aatcaggtct agcttctgga gctgggtctt
    12181 gatgtgcagg gtgagaagct tcattttgat gttcaggctt ggaaggggtt gagtctggtt
    12241 tctcttctgc agttgactca tggctagttc cattttgaga gactcgaaga acagtagctg
    12301 taagggcatt caatttcaag cctttttcag tccattcaag tcctttcggg ttggcaattc
    12361 cgactgatcc tgcttggttt tcatctgcca aaacttccgc atttcttaga tgtgcaaagg
    12421 cagttcccaa attaaattcg cgagcttttt catccgcatt gacaaagact gcgtagatat
    12481 caccgtttgg agcagtgatt tggtagccaa tcactacatc ctctttttcc acaccatttt
    12541 ggcctgggac agtgatgagg tggacacggt ctttgatatc ttgaagactc ttaagtcgga
    12601 aggcatctgt agattgacga agggcaatca aacctttcat atagtcacgg ctcttgacat
    12661 tttcaggata agctttacca tctgtagcct tagtccagtc aaacttgttg actgcgtcgc
    12721 tagaatcgta agaatcatgg atgaagtaag gatagtcaaa tgggttgccg tccttatcac
    12781 gcaacaagtg agatttgttt ggaaccttat cctctgctac tggagtcttg taggctgggt
    12841 cacggaattg tttagtacgt ccatattcct gaccggagtg gataaatgga gttccttgag
    12901 ctgtcaagac catgagattt ccaagtcgta aacgacggtg gatttcagca tagttctcag
    12961 ccttgcttgg gtcttttttg atagactggg caatgatgtc aaagagggtc aagttatcat
    13021 gggctgcgat gtattggatg acatctccag ggctgtcagc ttcaaagtta gttggttgag
    13081 caatgagatt tttaaagatg gtgttgacat cacgcttgcc acctgtgata aaggcaggtt
    13141 gaccttcgtt tggataacca gatttgaggt tgttacggat gtcatctgaa aagacagcga
    13201 cagtatcggt atgtttcatc caatcttggt cagcagcctt agtaggcatg ttttcatcac
    13261 cggcataggt tctccaacct tcaccaagca tgatgaggtt tggattgagg gcgcgtgcag
    13321 ccttgtaagc ttcttcgata gaagcggcgt catggtctcc catcatatcg aagcggaagc
    13381 catccacttt gtaggtatca actaggtatt tgatagagtc aactaggagc cgtttggtca
    13441 tatggtgggt tgtccccaag cgtccaccac caaagctagt tcgaggtgtg ccatcggcat
    13501 ccataaagtg gtagtagttt ggctctaggt cttcaaagat atcgactttg gctgtgtggt
    13561 tataaacgac atctaggata gctcccatac cacgtttgtg gatttcgttg atgaggtttt
    13621 taaattctgc gattcgtttt tctggattct taggatcgct tgagtacata ccagtcaagg
    13681 agaagtagtt ttgagggtca tatccccagt tgtagttgct gttgcttgaa gcgtagtcag
    13741 acaagcgttc atggtttttc aattcattga caaagtagta agacaagact ggaaggagct
    13801 ggatatgggt tacacccaag tctttgagat agtctagttt ttcaatgaag gcttcaaaag
    13861 tcccaaatgg tttggtcaag tcttttgcaa tggcaggatc tgaagtgaag tcacgcacat
    13921 gagcttcgta gataacggcg tcttcacgag tcttgaaatt gtgaatctta ccataagtca
    13981 agtcttgagg tccgagttta gctggatcta caaaggcggc tttagccact ttatgggcat
    14041 cgtcaatttt ggcatcgtcg ctattccaag cagcaagaga tttagcgtaa ggatcgagtg
    14101 caagaacagt tttaccttga cgctcgattt ggtattgata atagtagcca gtgaaatctg
    14161 tgattccgag tttgtttgtg ctgtctagag tttgtttcca agttcctctt tccccttttt
    14221 cgagagcgac agttccaact actttgtcag ggtcattctt gtcgtagaca acaacagaaa
    14281 ccttatcagc acttggtgac caaagggtca aatcaacttg ttttccttct tcttttaggt
    14341 cagctcccag tttgccatca tagctgtatg tctcatcttt caggcgccag cttgttttgg
    14401 tagtgaattg gtcggaattg tagctaacag tataaggatg ttttgtgtca gagaaatctc
    14461 cgctgtaggt cactttctta ccagcttcat cgattgcaac atcggtaata gttaccttgt
    14521 ttcctaggtg attagtgatg ttggagtgtt tgaggatatc ttctttttta gcaccgacaa
    14581 gtgttgaaaa gctactttca atgctagaag tgcctacgtg ttgggctcct gtcatacgga
    14641 tatcatggac atagtatgga tttgtgtaaa tcgattcatc atcgtctttt aggaaaattt
    14701 ggctatgatt tttcaaatct gtgaacttat aattttcttt acggattttc acgtcgtctc
    14761 cttgtttgct ctcatctagt aataaaaatc caaattctct tgcggcttca ttaagaggaa
    14821 tgtcgatata gcggccatat ttgcctgtag ccgtaaagtc tgttccgtca ggccattgag
    14881 cgctacttgg attttttaca tctccccagt accagagaga tttcttgtca tagttgccat
    14941 ctgtgcggta gtagttgacg cgaacagttc ctgcaggctg tggctcgtaa gagaaaacct
    15001 tgtaatcttg gtctaaccaa gcctcgttca tttttggaac tagtttttct acagatttat
    15061 cgccggttag attttttcca gctgtattgt tgatgaggaa gctaattttc ttggcttgtt
    15121 ctccctttaa tttgacatct aggtaatagc cgtagtcatc tttcttggca tccttgaagg
    15181 acaaagctcc gtttggccag ttttcagatg gtttttcaac atcgtcccaa gtccatagtc
    15241 cttgagcatc cttgttttct tcaggaagtt ttttgacatg gatacggaag tagttatctt
    15301 cgatgggctc ttctgcctta gtttcagttg ttactggact agtagaagta gttggctctg
    15361 ttgcaggttt ttcttctgtt ttctctaggg aagcgtttgc atttctaggt gaagaaatat
    15421 cactttcgtt cttaccagag ccagctgctg tatcagtaag aggttgggca agggtggttg
    15481 tatttctgtt gttgggttcg ttagtggttg gtgtactttc gtcggatgag atagttggtg
    15541 tagccatggc aagcaagaca aggcttgcgc cgataaggac agaaccagtt ccatttttga
    15601 gggaacggat gctgtagacc atttttttct cagtgtgaga tggtgttttt ctcataatga
    15661 ttctccaatc aataaatttc tatatcagta tagcatgtag taaaacaata tgcaagcgtt
    15721 ttctttgatt tgtgaaaaaa attcttactt ttgagaagtt ttctgtacta ttttatatat
    15781 cagtataaat gacggagata aaaatgtcac ataagccaag ccgtattatt aggaagatga
    15841 tgtgttccca gctattttta ttcagaaatt gatctctaac attaccaata aaaaagaaaa
    15901 atatcttgac aagcaaggga aaattttgtt acaataatag acggtacttt ttacttttgg
    15961 tctctcaaga gtgtacaggg acgtgctgac aaatgttgca aaagtacaca cagatgatat
    16021 ctgtcaccaa gtgtatcatc accaaaaata aaaaaacaca ggagaatgta gatgcctaca
    16081 attaaccaat tggttcgcaa accgcgtaaa tcaaaagtag aaaaatctaa atcaccagct
    16141 ttgaacgttg gttacaatag tcataaaaaa gttcaaacaa acgtttcttc accacaaaaa
    16201 cgtggtgttg caactcgtgt tggaacaatg acacctaaaa aacctaactc agcccttcgt
    16261 aaattcgctc gtgtacgttt gagcaacctt atcgaagtta ctgcctacat cccaggtatc
    16321 ggacacaact tgcaagagca cagcgtggtg cttcttcgcg gtggacgtgt aaaagacctt
    16381 ccaggggtac gttaccatat cgtccgtggt gcacttgata ctgcaggtgt taacgatcgt
    16441 aaacaaggcc gttctaaata cggtactaaa cgtccaaaag cataaggaaa ggggataaag
    16501 agaaatgagt cgtaaaaata gagctccaaa acgtgacgta ttgccagatc cgctatacaa
    16561 ttcacaacta gttactcgtc ttatcaaccg cgttatgctt gatggtaaac gtggtactgc
    16621 tgcttcaatc gtttacggtg cttttgagca aatcaaagaa gctactggca acgatgcact
    16681 tgaagtattt gaaacagcta tggaaaacat catgcctgta cttgaagtac gtgcacgtcg
    16741 tgttggtggt tctaactacc aagtcccagt tgaagttcgt ccagaacgtc gtacaacact
    16801 tggacttcgt tggttggtaa caatcgctcg tcttcgtggt gaacacacaa tgcaagaccg
    16861 tcttgcaaaa gaaatcttgg atgctgctaa caacactggt gcagcagtta agaaacgtga
    16921 agatactcac cgtatggctg aagctaaccg tgcattcgca cacttccgtt ggtaagatag
    16981 gatgcgaaag cgttaagaaa gtcccagaga aaatagggaa tcgaagcagg ttgcggttgc
    17041 aaccaatgag attcatcttt ttctccagac ttttagcttg agctcaacta aatcatgatg
    17101 ctaggcacgg taaggatgca aggtgaaaat aggaaactga cgcagtattc aacgaataca
    17161 aggagtttta tctttttcac gcagcatccc gttccagctc acatcggcta actaacttta
    17221 gcctgggttc aaattagcta aatcgattag tattagctat aactcagctt accatctcgt
    17281 aagttgaaac caacaatagc atgaaaacat tgagaacggg taggtcctgc ctatccgttt
    17341 ttattaaaat cgtgttataa tagaatagaa atcaaaaata aataggagaa acaaacctca
    17401 tggcacgcga attttcactt gaaaaaactc gtaatatcgg tatcatggct cacgtcgatg
    17461 ccggtaaaac aacaactact gagcgtattc tttactacac tggtaaaatc cacaaaatcg
    17521 gtgaaactca cgaaggtgcg tcacaaatgg actggatgga gcaagagcaa gaacgtggta
    17581 tcacgatcac atctgctgcg acaacagctc aatggaacaa ccaccgcgta aacatcatcg
    17641 acacaccagg acacgtggac ttcacaatcg aagtacaacg ttctcttcgt gtattggatg
    17701 gtgcggttac cgttcttgac tcacaatcag gtgttgaacc tcaaactgaa acagtttggc
    17761 gtcaagcaac tgagtacgga gttccacgta tcgtatttgc caacaaaatg gacaaaatcg
    17821 gtgctgactt cctttactct gtaagcacac ttcacgatcg tcttcaagca aatgcacacc
    17881 caatccaatt gccaatcggt tctgaagatg acttccgtgg tatcattgac ttgatcaaga
    17941 tgaaagctga aatctatact aacgaccttg gtacggatat ccttgaagaa gacatcccag
    18001 ctgaatacct tgaccaagct caagaatacc gtgaaaaatt gattgaagca gttgctgaaa
    18061 ctgacgaaga attgatgatg aaatacctcg aaggtgaaga aatcactaac gaagaattga
    18121 aagctggtat ccgtaaagcg actatcaacg ttgaattctt cccagtattg tgtggttcag
    18181 ccttcaaaaa caaaggtgtt caattgatgc ttgatgcggt tatcgactac cttccaagtc
    18241 cacttgacat cccagcaatc aaaggtatta acccagatac agacgctgaa gaaactcgtc
    18301 cagcatctga cgaagagcca tttgcagctc ttgccttcaa gatcatgact gacccattcg
    18361 taggtcgttt gacattcttc cgtgtttact caggtgttct tcaatcaggt tcatacgtat
    18421 tgaatacttc taaaggtaaa cgtgaacgta tcggacgtat ccttcaaatg cacgctaaca
    18481 gccgtcaaga aatcgacact gtttactcag gtgatatcgc tgctgccgtt ggtttgaaag
    18541 atactacaac tggtgactca ttgacagatg aaaaatctaa aatcatcctt gagtcaatca
    18601 acgttccaga accagttatc caattgatgg ttgagccaaa atctaaagct gaccaagaca
    18661 agatgggtat cgcccttcaa aaattggctg aagaagatcc aacattccgc gttgaaacaa
    18721 acgttgaaac tggtgaaaca gttatctcag gtatgggtga acttcacctt gacgtccttg
    18781 ttgatcgtat gcgtcgtgag ttcaaagttg aagcgaacgt aggtgctcct caagtatctt
    18841 accgtgaaac attccgcgct tctacccaag cacgtggatt cttcaaacgt cagtctggtg
    18901 gtaaaggtca attcggtgat gtgtggattg aatttactcc aaacgaagaa ggtaaaggat
    18961 tcgaattcga aaacgcaatc gtcggtggtg tggttcctcg tgaatttatc ccagcggttg
    19021 aaaaaggttt ggtagaatct atggctaacg gtgttcttgc aggttaccca atggttgacg
    19081 ttaaagctaa gctttatgat ggttcatatc acgatgtcga ctcatctgaa actgccttca
    19141 agattgcggc ttcactttcc cttaaagaag ctgctaaatc agcacaacca gctatccttg
    19201 aaccaatgat gcttgtaaca atcactgttc cagaagaaaa ccttggtgat gttatgggtc
    19261 acgtaactgc tcgtcgtgga cgtgtagatg gtatggaagc acacggtaac agccaaatcg
    19321 ttcgtgctta cgttccactt gctgaaatgt tcggttacgc aacagttctt cgttctgcat
    19381 ctcaaggacg tggtacattc atgatggtat ttgaccacta cgaagatgta cctaagtcag
    19441 tacaagaaga aattattaag aaaaataaag gtgaagacta atccgtcctc actctagaag
    19501 gaagtcactt agtggcttcc ttttgtcttt agaaaatacc tctaaatatg gtaaaatagt
    19561 agaagaataa tgtgaggaaa atgaatgtca aatagttttg aaattttgat gaatcaattg
    19621 gggatgcctg ctgaaatgag acaggctcct gctttagcac aggccaatat tgagcgagtt
    19681 gtggttcata aaattagtaa ggtatgggag tttcattttg tattttctaa tattttaccg
    19741 attgaaatct ttttagaatt aaagaaaggt ttgagcgaag aattttctaa gacaggcaat
    19801 aaagctgttt ttgaaattaa ggctcggtct caagaatttt caaatcagct cttgcagtcc
    19861 tactataggg aggctttctc tgaaggtcca tgtgctagtc aaggttttaa gttcctttat
    19921 caaaatttgc aagttcgtgc tgagggtaat cagctattta ttgaaggatc tgaagcgatt
    19981 gataaggaac attttaagaa gaatcatctt cctaatttag ctaaacaact tgaaaagttt
    20041 ggttttccaa cttttaactg tcaagtcgag aagaatgatg tcctgaccca agagcaggaa
    20101 gaggcctttc atgctgaaaa tgagcagatt gttcaagctg ccaatgagga agcgctccgt
    20161 gctatggaac aactggagca gatggcacct cctccagcgg aagagaaacc agtctttgat
    20221 tttcaagcga aaaaagctgc agctaaaccc aagctggata aggcggagat tactcctatg
    20281 atcgaagtga cgacagagga aaatcgtctg gtatttgaag gggttgtttt tgatgtggag
    20341 caaaaagtga ctagaacagg tcgtgtttta atcaacttta aaatgacgga ctatacttca
    20401 agtttttcta tgcaaaagtg ggttaaaaac gaggaagagg cccagaagtt tgacctcatc
    20461 aagaagaatt cttggctccg agttcgaggg aatgtggaga tgaataactt cacacgcgat
    20521 ttgactatga acgtacagga tctgcaggaa gttgttcact atgagcggaa ggatttgatg
    20581 ccagaaggtg agcgtcgggt tgagtttcat gctcatacta acatgtcgac tatggatgct
    20641 ttgccagagg tcgaagagat tgttgcaaca gctgctaagt ggggacacaa ggcggttgct
    20701 atcacagacc atgggaatgt ccagtccttt ccacatggct ataaggcggc taagaaagcg
    20761 ggaatccagc tgatctatgg gatggaagcc aatatcgtgg aggaccgtgt ccctatcgtc
    20821 tataacgaag tggagatgga cttatcagaa gcaacctacg tggtctttga cgtggaaacg
    20881 acgggacttt cagctatcta taatgacttg attcaggttg cggcctctaa gatgtacaag
    20941 gggaatgtta ttgctgaatt tgatgaattt atcaatcctg ggcatccctt gtcagctttt
    21001 actacagagt taactggaat tacagatgat catgtcaaaa atgccaaacc actagaacaa
    21061 gttttgcaag aattccaaga attttgcaag gatacggtcc tagttgccca caatgctacc
    21121 tttgacgttg gctttatgaa tgctaattat gagcgtcatg atcttccaaa gattagtcag
    21181 ccagttattg atacgctgga gtttgctaga aacctctatc ctgagtataa acgccatggt
    21241 ttggggcctt tgaccaagcg ttttggtgtg gccttggaac atcaccacat ggccaactac
    21301 gatgcggaag cgactggtcg tctgcttttc atctttatca aagaggtagc agaaaaacat
    21361 ggtgtgaccg atttagctag actcaacatt gatctaatca gtccagattc ttacaaaaaa
    21421 gctcggatca agcatgcgac catctatgtc aagaatcagg taggtctaaa aaatatcttt
    21481 aagctggttt ccttgtctaa taccaagtat tttgaaggag tgccacggat tccgagaacg
    21541 gttctagatg cccatcgaga gggcttgatt ttaggttcag cctgttcaga gggtgaagtt
    21601 tttgacgtgg tcgtttctca aggtgtggat gcggcggttg aggtggccaa gtattatgac
    21661 tttatcgagg tcatgccacc ggctatctat gcgcccttga ttgccaaaga gcaggtcaag
    21721 gatatggagg aactccagac cattatcaag agtttgatag aggttggaga tcgccttggc
    21781 aagcctgttc tggctacggg aaatgttcac tatatcgaac cggaagaaga gatttatcgt
    21841 gaaattatcg tccgtagttt gggacagggt gcgatgatta accgaactat cggtcatggt
    21901 gaacatgccc aaccagcgcc acttccaaag gctcattttc gaacgactaa tgagatgttg
    21961 gatgaatttg cctttttggg agaggaactg gctcgtaaac tggttattga aaacaccaat
    22021 gccttggcag aaatatttga acccgttgaa gtcgttaagg gtgacttgta tacgcctttc
    22081 atcgacaagg ctgaagaaac agttgctgag ttgacctata agaaagcttt tgagatttat
    22141 ggaaatccgc tgccagatat tgttgatttg cggattgaaa aagaattaac atccatactg
    22201 gggaatggat ttgctgtgat ttatctggca tcgcagatgc tggtgcaacg ttctaatgaa
    22261 cggggttatt tggttggttc tcgtgggtct gtcggatcta gtttcgttgc gaccatgatt
    22321 gggattacgg aggtcaatcc tctctctcct cactatgtct gtggtcagtg tcagtacagt
    22381 gagtttatca cagatggttc gtacggttca ggatttgata tgccccataa ggactgtcca
    22441 aactgtggtc acaaactcag taaaaacgga caggatattc cgtttgagac cttccttggt
    22501 tttgatgggg ataaggttcc tgatattgac ttgaacttct cgggagaaga tcagcctagc
    22561 gcccacttgg atgtgcgtga tatctttggt gaagaatatg ccttccgtgc gggaacggtt
    22621 ggtacggtag ctgccaagac tgcctatgga tttgtcaagg gttacgagcg agattatggc
    22681 aagttttatc gtgatgcaga agtagaacgc ctcgctcaag gagcggcggg tgtcaagcgg
    22741 acaacaggcc aacacccggg gggaatcgtt gttattccga actacatgga tgtctacgat
    22801 tttacgcctg tccagtatcc agcagatgat gtcacggctg aatggcagac cactcacttt
    22861 aacttccacg atatcgatga gaacgtcctc aaactcgatg tactgggaca tgatgatccg
    22921 accatgattc gaaaacttca ggatttgtct ggtattgacc ctaataaaat tcctatggat
    22981 gacgaaggcg tgatggcact cttttctggg actgatgtgc taggggtaac acctgaacaa
    23041 attggaacgc ctacgggtat gttggggatt ccagagtttg gaacaaattt cgtacgtgga
    23101 atggtagacg aaacccatcc gacaaccttt gcggaattgc ttcagctgtc tggtctgtcc
    23161 cacggtactg atgtttggtt ggggaatgct caggatctga ttaagcaagg aatagcggac
    23221 ctatcgactg ttatcggttg tcgggacgac atcatggttt acctcatgca tgcgggtctg
    23281 gaacctaaga tggcctttac cattatggaa cgggtacgta agggtttgtg gctaaagatt
    23341 tcagaagagg agagaaatgg ctatatcgaa gcaatgaagg ctaataaggt gccagagtgg
    23401 tatatcgaat cctgtgggaa aattaagtac atgttcccta aggcccatgc ggcagcctac
    23461 gttatgatgg ccttgcgtgt agcttacttc aaggttcacc atcctattta ttactactgt
    23521 gcttacttct ccattcgtgc taaggctttt gatatcaaga ccatgggtgc gggcttggag
    23581 gtcatcaagc gcagaatgga agaaatctct gaaaaacgga agaacaatga agcctctaat
    23641 gtggaaatcg atctctatac aactcttgag attgtcaatg agatgtggga acgaggtttc
    23701 aagtttggta aattagatct ctaccgtagt caggcgacag agttcctcat cgacggggat
    23761 acccttatcc caccatttgt agcaatggat ggtctgggag agaacgttgc caagcaactg
    23821 gtgcgggcgc gtgaagaggg agaattcctc tctaaaacag aactacgcaa gcgtggtgga
    23881 ctctcatcaa ccttggttga aaagatggat gagatgggta ttcttggaaa tatgccagag
    23941 gataaccagt tgagtttgtt tgatgagttg ttttaaaaaa ttgcttaata atctattaaa
    24001 agaggctaac gtatatccaa tagatttaca ttagctttct tttttgttaa aatagtctat
    24061 ggaaagaggg tgagagtatg tcaaagatga gtataagcat ccgtctggat agtgaggtta
    24121 aggagcaggc ccaacaggtg tttagtaatc tgggaatgga tatgacaaca gctattaata
    24181 ttttccttcg tcaggcaatt caatatcagg gattaccttt tgatgttaga ctagacgaaa
    24241 atcggaagtt gctccaagcg ttaacggatt tagaccaaaa tcgtaatatg agccagtctt
    24301 ttgaatcagt ctcagatttg atggaggact tacgtgctta agattcgtta tcataaacag
    24361 tttaaaaaag attttaagtt ggctatgaag cgtggtttga aggcagaatt attagaagaa
    24421 gttttgaatt ttctggttca agaaaaagaa catcctgcca gatatcgtga tcattcattg
    24481 acggcatcca agcattttca aggagttcgt gaatgccata cccagccaga ttggcttttg
    24541 gtttataaag tagacaagtc ggaattgatt ttaaatttgc tgaggacagg cagtcacagt
    24601 gatttatttt aatctatttt aagggggttc tcatgaaact aagaatattt gcggaagata
    24661 agccggctaa gaaggtattt gaatatcaat tagaacttgc tgatcgtaca attcttctat
    24721 cgacagcact cttgtcaggt gctattgctt tagcaggaat cttttctgct ttgaaagaaa
    24781 aataaaaata gaaaagagaa aacagaatgg ttttaccaaa ttttaaagaa aatctagaaa
    24841 aatatgcgaa attgttggtt gcgaacggaa ttaacgtgca acctggtcac actttggctc
    24901 tctctattga tgtggagcaa cgtgaattgg cacatctaat cgtgaaagaa gcttatgcct
    24961 tgggtgcgca tgaggtcatc gttcagtgga cagatgatgt gattaaccgt gagaaattcc
    25021 tccatgcccc gatggagcgt ttggacaatg tgccagaata caagattgct gagatgaact
    25081 atcttttgga gaacaaggct agccgtcttg gggttcgttc atctgatcca ggtgccttga
    25141 acggagtgga cgctgacaag ctttcagctt ctgctaaagc tatgggactt gccatgaagc
    25201 ctatgcgtat cgcaactcaa tctaacaagg ttagctggac tgtagcagcc gctgctggac
    25261 ttgagtgggc taagaaagtc ttcccaaatg ctgcgagcga cgaagaagca gttgatttcc
    25321 tttgggatca aatcttcaaa acttgtcgtg tctacgaagc agatcctgtt aaggcttggg
    25381 aggaacatgc agccattctc aagagcaagg ccgatatgct taataaggag caattttcag
    25441 cccttcacta cacagcgcca ggaacagatt taacacttgg tttgccaaag aaccacgttt
    25501 gggaatcagc tggtgctgtc aatgcacagg gcgaagaatt cttgccaaat atgccgacag
    25561 aagaggtctt cacagcgcct gacttccgtc gtgcagatgg ttatgtcact tctacaaaac
    25621 cgcttagcta caacggaaat atcattgaag gcattaaggt gacctttaag gatggacaaa
    25681 tcgtagatat cactgctgag aagggtgatc aggttatgaa agaccttgtc tttgaaaatg
    25741 cgggtgcgcg tgccttgggt gaatgtgcct tggtaccaga tccaagtcca atttctcagt
    25801 caggcattac cttttttaac acccttttcg atgaaaatgc gtcaaaccac ttggctatcg
    25861 gtgcagccta tgcgactagc gttgttgatg gagcggagat gagcgaagag gagcttgaag
    25921 ctgcagggct taaccgttca gatgttcacg tagactttat gattggttct aaccaaatgg
    25981 atatcgatgg tattcgtgag gatggaacgc gggtacctct tttccgtaat gggaattggg
    26041 caaattaagg agataatatg ttaggaagta tgttcgttgg tctcctagtg ggatttttag
    26101 caggtgctat gaccaatcgt ggagagcgaa tgggatgttt tggaaaaatg tttctcggtt
    26161 ggatcggagc ctttctaggt cacttgctct ttggaacttg ggggccagtt ttatcaggaa
    26221 cagctattat cccagcgatt ttaggagcca tgattgtttt agctattttt tggagacgag
    26281 gaagttaatt tcctaaatct gatactcaat gaaaatcaaa gagcaaacta ggaaactagc
    26341 cgcaggttgc tcaaagcact gctttgaggt tgcagataga actgacgaag tcagctaaaa
    26401 acactgtttt gaggttgcag atagaactga cgaagtcagt aacatatata cggcaaggcg
    26461 acgttgacgc ggtttgaaga gatttttgaa gagtatgaaa cgaaaaggag gttggtcatt
    26521 gtactagcct ccttttgata tgatataata gttctatgag attagataaa tttttagttg
    26581 cctgtgcggt gggaagtcgg actgaggtca aaaacttgct caaggctggg cgcgtgactg
    26641 taaatggtaa aaaagaaaaa tcagctaaat tgcagattga tgaaaaaata gatgagattc
    26701 gctttgatgg gcaagtgttg gagtatgaag agtttgtcta ctacatgatg aacaagccca
    26761 aaggagttat ctcagcgact gaggatccca agcacagaac cgttctggac ttgttggatg
    26821 acttggcgcg gagtaaggaa gttttcccag taggacgctt ggatattgac acgcatggtc
    26881 ttttgctctt gaccaatgat ggtcagctgg ctcatgttct tctttcgccc aagcgtcatg
    26941 tggacaagac ttatctggca caagtcaagg gaatcatgac ccaagaagat gtggagacat
    27001 ttgctgaggg tattcctctc aaagacttta cctgtcaacc cgctatactg gagcttgtat
    27061 ccatagatac agaaaagaat caaagccaaa tccgtgtgac cattgcagaa gggaagtttc
    27121 atcagatcaa gcgtatggtg ggctactgtg gcaaggaagt ggtggacttg caacgattga
    27181 ctatgggaac tttagtatta gatgagaacc tggaacgagg ggaatggcgt cgcttgacca
    27241 aggaagaatt agaaattctc cgcgctaata ttatttaatt tagt
//
LOCUS       DAVLSL010000013        26936 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLSL010000013 DAVLSL010000000
VERSION     DAVLSL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.013"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..743)
                     /locus_tag="VXN30_000428"
     CDS             complement(join(<1..373,375..743))
                     /locus_tag="VXN30_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1357101.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTIMIDVTEVKINRPKKELANYSGKKKCYAMKAQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKFKPLTVEDKTYNHA
                     LSKERSKVENIFAKVETFKMFSTTYRNHR"
     gene            738..992
                     /locus_tag="VXN30_000429"
     CDS             738..992
                     /locus_tag="VXN30_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1357102.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1287..2621
                     /gene="trmFO"
                     /locus_tag="VXN30_000430"
     CDS             1287..2621
                     /gene="trmFO"
                     /locus_tag="VXN30_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1357103.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2705..3442
                     /locus_tag="VXN30_000431"
     CDS             2705..3442
                     /locus_tag="VXN30_000431"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1357104.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3451..4008
                     /gene="frr"
                     /locus_tag="VXN30_000432"
     CDS             3451..4008
                     /gene="frr"
                     /locus_tag="VXN30_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1357105.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            4068..4922
                     /gene="cvfB"
                     /locus_tag="VXN30_000433"
     CDS             4068..4922
                     /gene="cvfB"
                     /locus_tag="VXN30_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1357106.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4931..5146
                     /locus_tag="VXN30_000434"
     CDS             4931..5146
                     /locus_tag="VXN30_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1357107.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5232..6200
                     /locus_tag="VXN30_000435"
     CDS             5232..6200
                     /locus_tag="VXN30_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1357108.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(6178..6357)
                     /locus_tag="VXN30_000436"
     CDS             complement(6178..6357)
                     /locus_tag="VXN30_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357109.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6393..6894
                     /locus_tag="VXN30_000437"
                     /pseudo
     CDS             6393..6894
                     /locus_tag="VXN30_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            6897..7157
                     /locus_tag="VXN30_000438"
                     /pseudo
     CDS             6897..7157
                     /locus_tag="VXN30_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7524..7850)
                     /locus_tag="VXN30_000439"
     CDS             complement(7524..7850)
                     /locus_tag="VXN30_000439"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357110.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7796..8122)
                     /locus_tag="VXN30_000440"
     CDS             complement(7796..8122)
                     /locus_tag="VXN30_000440"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1357111.1"
                     /translation="MIGDGVVGTHAARIALGLGAQVTILDISAKRLSVLEEVFGSQIQ
                     TLISNSFNIEASVRDADVVIGAILIPGAKAPELVTDEMVKQMRPGSVSLTLLLTKVAL
                     SKQLTV"
     gene            complement(8209..8634)
                     /locus_tag="VXN30_000441"
     CDS             complement(8209..8634)
                     /locus_tag="VXN30_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1357112.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLNSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8811..9380
                     /locus_tag="VXN30_000442"
     CDS             8811..9380
                     /locus_tag="VXN30_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357113.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            9448..10098
                     /locus_tag="VXN30_000443"
     CDS             9448..10098
                     /locus_tag="VXN30_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1357114.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEEEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            10082..12322
                     /locus_tag="VXN30_000444"
     CDS             10082..12322
                     /locus_tag="VXN30_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1357115.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GYLNTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLIGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPKLTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGWNSW
                     KIESVR"
     gene            12464..12688
                     /locus_tag="VXN30_000445"
     CDS             12464..12688
                     /locus_tag="VXN30_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357116.1"
                     /translation="MIIGLFQSSISAVTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSW
                     SRYYSYSEYPVGSGWNYARYEVINYYTGGY"
     gene            12720..13307
                     /locus_tag="VXN30_000446"
     CDS             12720..13307
                     /locus_tag="VXN30_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357117.1"
                     /translation="MLRLTHVTLKTRQVILQDADFTFKKGRVYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            13311..14492
                     /locus_tag="VXN30_000447"
     CDS             13311..14492
                     /locus_tag="VXN30_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357118.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    14652..14783
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14799..15329
                     /gene="infC"
                     /locus_tag="VXN30_000448"
     CDS             14799..15329
                     /gene="infC"
                     /locus_tag="VXN30_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1357119.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15362..15562
                     /gene="rpmI"
                     /locus_tag="VXN30_000449"
     CDS             15362..15562
                     /gene="rpmI"
                     /locus_tag="VXN30_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1357120.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15614..15973
                     /gene="rplT"
                     /locus_tag="VXN30_000450"
     CDS             15614..15973
                     /gene="rplT"
                     /locus_tag="VXN30_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1357121.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            16031..16411
                     /locus_tag="VXN30_000451"
     CDS             16031..16411
                     /locus_tag="VXN30_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1357122.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16661..17461
                     /locus_tag="VXN30_000452"
     CDS             16661..17461
                     /locus_tag="VXN30_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1357123.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            17424..18410
                     /locus_tag="VXN30_000453"
     CDS             17424..18410
                     /locus_tag="VXN30_000453"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1357124.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18452..20485
                     /locus_tag="VXN30_000454"
     CDS             18452..20485
                     /locus_tag="VXN30_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV1357125.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMTSD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(20546..22201)
                     /gene="pavA"
                     /locus_tag="VXN30_000455"
     CDS             complement(20546..22201)
                     /gene="pavA"
                     /locus_tag="VXN30_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1357126.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            22306..22803
                     /gene="ybeY"
                     /locus_tag="VXN30_000456"
     CDS             22306..22803
                     /gene="ybeY"
                     /locus_tag="VXN30_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1357127.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22784..23179
                     /locus_tag="VXN30_000457"
     CDS             22784..23179
                     /locus_tag="VXN30_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1357128.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            23196..24095
                     /gene="era"
                     /locus_tag="VXN30_000458"
     CDS             23196..24095
                     /gene="era"
                     /locus_tag="VXN30_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1357129.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            24143..24967
                     /gene="mutM"
                     /locus_tag="VXN30_000459"
     CDS             24143..24967
                     /gene="mutM"
                     /locus_tag="VXN30_000459"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1357130.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24967..25572
                     /locus_tag="VXN30_000460"
     CDS             24967..25572
                     /locus_tag="VXN30_000460"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1357131.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEAIVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25559..26758
                     /locus_tag="VXN30_000461"
     CDS             25559..26758
                     /locus_tag="VXN30_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1357132.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRIFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26758..26907
                     /gene="rpmG"
                     /locus_tag="VXN30_000462"
     CDS             26758..26907
                     /gene="rpmG"
                     /locus_tag="VXN30_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1357133.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
BASE COUNT         7899 a         5041 c         6099 g         7897 t
ORIGIN      
        1 tacgatgatt tcgataggtt gttgaaaaca ttttaaacgt ttctactttg gcaaagatgt
       61 tctcaacctt gcttctctcc ttagatagcg catggttata ggttttatct tcaactgtta
      121 gcggcttgaa tttgctggat ttacgtggag tttgtgcttg aggatatatc ttcatgagcc
      181 cttgataacc actgtcagcc aagattttac cagcttgtcc gatatttctg cgactcattt
      241 tgaacaactt catatcatga caatagttca cagtgatatc caaagaaaca attctccctt
      301 gacttgtgac aatcgcttga gccttcatag cgtagcattt ctttttacca gaataattcg
      361 ctaattcttt ttttagggcg attgattttt acttccgtca catcaatcat tatcgtgtcc
      421 tcagaactga gaggagttct tgaaatcgta acaccacttt gaacaagagt tacttcaacc
      481 cattggctcc gacggattaa gttgctttcg tgaataccaa aatcagccgc aatttgctca
      541 taagttcgat attctcgcac atattgaaga gtggccataa gaaggtcttc taggcttaat
      601 ttaggttttc gtccaccttt tgcgtgttta agttgataag ctgtttttaa tacagctaat
      661 atctcttcaa aagtcgtgcg ctgaacacca acaagacgct taaatcgtgc atcagttagt
      721 tgtttacttg cttcatcatt catagaacta ctataccata ttttgtttcg caggaagtct
      781 attggaaagt aagaaatatt gaagctgagg cgattagaag aaattgtgag cgtggtgcta
      841 ttttttcagg taaaataaaa catcacgaag attctcagtt taaaggagac cactatgttg
      901 aatgttatgc tgttttagat aatacggtta tagcaagaga tagaataaca gtccctatcg
      961 atccgttatg tggaaaagat tttatagagt agcatataat tgattcttaa ctggaatact
     1021 ctctatctct ttacatcaag aaaatgacta aacagggaag tttgccttct tccctttttt
     1081 tgttatacta gtagaagaaa aaaatagaaa gatttgtggg agtgaaaaag tccgggggac
     1141 tttttcagct tgagccaaga aattcgagag cgaggaaagt ctgggggact ttttcagcct
     1201 gagccaagaa actcgaaatc tcgtaagtct gattggcttt ttcaatgtga atcttaactt
     1261 cacactccca aagaggtatt agtgtcgtgt ctcaatctta tatcaatgtt atcggtgctg
     1321 gtttggcagg ttctgaagca gcttaccaaa tcgcagagcg tggtattcca gttaaacttt
     1381 atgaaatgcg tggtgtcaag tctacacccc agcataaaac agacaatttt gctgagttgg
     1441 tttgttccaa ttctttgcgt ggggatgctt tgacaaatgc agttggtctt ctcaaggaag
     1501 aaatgcgtcg cttgggttct gttatcttgg aatctgctga ggctacacgt gttcctgccg
     1561 gtggagccct tgcggtggat cgtgatggtt tctctcaaat ggtgaccgaa aaagtggtca
     1621 accacccctt gattgaagtg gtgcgtgatg aaattacaga attgccaaca gatgttatta
     1681 cagttgtggc gactggtccc ttgactagcg atgccctggc tgaaaagatt catgccctta
     1741 ataatggcga tggcttctat ttctacgatg cagcagcgcc tattatcgat gtcaacacta
     1801 tcgatatgag taaggtctat ctcaaatctc gttatgataa gggagaagca gcctatctca
     1861 atgctccaat gaccaagcaa gagtttatgg atttccatga agccttggtc aatgcggaag
     1921 aagcaccgct caattctttt gaaaaagaaa agtactttga aggatgtatg ccaatcgaag
     1981 ttatggccaa acgtggcatt aaaactatgc tttatggccc tatgaagcca gtcggtcttg
     2041 agtatccgga cgactacaca ggacctcgtg atggagaatt taaaacacct tatgcggttg
     2101 tgcaacttcg tcaggataat gcagctggta gcctatacaa tattgttggt ttccagaccc
     2161 acctcaaatg gggagaacaa aagcgtgtct tccaaatgat tccgggtctt gaaaatgcgg
     2221 agtttgtccg ttatggtgtc atgcatcgca attcttacat ggattcacca aatcttcttg
     2281 agcagactta ccgttccaag aaacaaccaa atctcttctt tgctggtcag atgacggggg
     2341 tggaaggcta tgttgagtcg gcggcttcgg gcttagttgc gggaattaac gcagctcgtc
     2401 tcttcaagga agaaagcgag gttattttcc ccgagacgac agcgattgga agcttagctc
     2461 attacattac ccatgccgac agcaaacatt tccaaccaat gaatgtcaat tttgggatca
     2521 tcaaggagtt ggaaggcgag cgtatccgtg ataagaaggc tcgttatgaa aaaattgcag
     2581 agcgtgccct tgccgactta gaggaatttt tgactgtcta atttttttga aagaattgtt
     2641 catgatacta taaaaatctt agaaattgtg ataaaatagg taggatgaaa gaaggagagt
     2701 gaaaatggcg aatcccaagt ataaacgtat tttaatcaag ttatcaggtg aagcccttgc
     2761 cggtgaacgt ggcgtaggga ttgatatcca aacagttcaa acaatcgcaa aagagattca
     2821 agaagttcat agcttaggta tcgaaattgc ccttgttatt ggtggaggaa atctctggcg
     2881 tggagaacct gcagcagaag caggtatgga ccgtgttcag gcagattaca ctggaatgct
     2941 tgggactgtt atgaatgctc ttgtgatggc agattcattg caacaagttg gggttgatac
     3001 gcgtgtacaa acagctattg ctatgcaaca agtggcagag ccttatgtcc gtggacgtgc
     3061 ccttcgtcac cttgaaaaag gccgtatcgt tatctttggt gctggaattg gttcaccata
     3121 cttctcgaca gatacaacag cggcccttcg tgcagctgaa atcgaagcag atgccatcct
     3181 catggctaaa aatggcgtcg atggtgtgta caatgccgat cctaagaagg acaagacagc
     3241 cgttaagttt gaagaattga cccaccgtga tgttatcaac aaaggtcttc gtatcatgga
     3301 ctcaacagcc tcaaccctct caatggacaa cgacattgac ttggttgtct tcaacatgaa
     3361 ccaatcaggc aacatcaaac gtgttgtatt tggtgaaaat atcggaacaa cagtttcaaa
     3421 taatatcgaa gaaaaggaat aagaaagaat atggctaacg taattattga aaaagctaaa
     3481 gagagaatga cccagtctca ccaatcactt gctcgtgaat ttggtggtat ccgtgctggt
     3541 cgtgccaatg caagcttgct tgaccgtgta catgtagaat actatggagt cgaaactcct
     3601 cttaaccaaa tcgcttcaat tacgattcca gaagcgcgtg ttttgttggt aacaccattt
     3661 gacaagtctt cattgaaaga catcgaacgt gccttgaacg cttctgatct tggtatcaca
     3721 ccggctaatg acggttctgt gattcgcttg gttatcccag ctcttacaga agaaactcgt
     3781 cgtgaccttg ctaaagaagt gaagaaggtc ggcgaaaatg ctaaagtggc tgtccgcaat
     3841 atccgtcgcg atgctatgga cgaagctaag aagcaagaaa aagcaaaaga aatcactgaa
     3901 gacgaattga agactcttga aaaagatatt caaaaagtaa cagacgatgc tgttaaacac
     3961 atcgacgaca tgactgctaa caaagagaaa gaacttttgg aagtctaaaa ataaacagaa
     4021 aaactcagtt ggcattgctg gctgagtttt attcgaaaga aggaaatatg aatacaaatc
     4081 ttgcaagttt tatcgttgga ctgatcatcg atgaaaatga ccgtttttac tttgtgcaaa
     4141 aggatggtca aacctatgct cttgctaagg aagagggcca acatacagta ggggatacgg
     4201 tcaaaggttt tgcatacacg gatatgaagc aaaaactccg cctgacaacc ttagaagtga
     4261 ctgccactca ggaccaattt ggttggggac gtgtcacaga ggttcgtaag gacttgggtg
     4321 tctttgtgga tacaggcctt cctgacaagg aaatcgttgt gtcactcgat attctccctg
     4381 tgctcaagga actctggcct aagaagggcg accaactcta catccgtctt gaagtggata
     4441 agaaagaccg tatctggggc ctcttggctt atcaagaaga cttccaacgt ctcgctcgtc
     4501 ctgcctacaa caacatgcag aaccaaaact ggccagccat tgtttaccgt ctcaagctgt
     4561 caggaacttt tgtttaccta ccagaaaata atatgcttgg ttttattcat cctagcgagc
     4621 gttacgcaga gccacgtttg gggcaagtat tagatgcgcg cgttattggt ttccgtgaag
     4681 tggaccgcac tctgaacctc tccctcaaac cacgctcctt tgaaatgttg gaaaacgatg
     4741 ctcagatgat tttgacttat ttggaaagca atggcggttt catgacctta aatgacaagt
     4801 catctccaga cgacatcaag gcaacctttg gcatttctaa aggtcagttc aagaaagctt
     4861 taggtggtct tatgaaggct ggtaaaatca agcaagacca gtttgggaca gagttgattt
     4921 agggaggctt atgagaaaat cattttacac ttggctcatg accgagcgca atcctaaaag
     4981 taacagtccc aaagcaattt tggcagacct cgcttttgaa gagtcagcct ttccaaaaca
     5041 tacagatgat tttgatgagg tcagtcgctt tttggaggag catgccagtt tctcttttaa
     5101 cctaggagat tttgacagca tttggcagga atatctagaa cactagcatt tattcattgg
     5161 gtttgggcta gtaatttctc catccctctg ctataataaa aagaaataaa aggattagag
     5221 aggttcttta tttgaaggaa cattcaatag acattcaact gagtcatcca gatgacctgt
     5281 ttcatctttt tggttccaat gaacgccatc ttcgtttgat ggaagaagag cttgatgttg
     5341 tgattcatgc tcgtacggag attgtccagg ttttgggaga agagtctgcc tgtgaggaag
     5401 cccgtcaagt tattcaggct ttgatggtct tggtaaatcg tgggatgacc gttggtacgc
     5461 cagatgtagt cactgcgatt agcatggtca aaaatgatga aattgacaag tttgtcgccc
     5521 tttacgaaga agaaatcatc aaagacaata ctgggaagcc tatccgagtc aaaaccttgg
     5581 gtcaaaaact ttatgtggac agtgtcaaac agcatgatgt gacctttgga atcggacctg
     5641 cagggacagg gaagaccttc cttgcagtga ccttggcagt gactgccctt aaacgtgggc
     5701 aagtcaaacg aattatcctg acacgcccag cggtggaagc aggagagagt ctaggttttc
     5761 ttccgggtga tcttaaggag aaggtggatc cttaccttcg tcctgtttac gatgccttgt
     5821 atcaaattct tgggaaagac caaacgactc gtctcatgga gcgtgaaatt atcgaaattg
     5881 cgccccttgc ctatatgcgt ggccggacct tggatgatgc ctttgtcatt ctcgatgagg
     5941 cgcaaaacac gaccatcatg cagatgaaga tgttcttgac gcgtttaggc tttcattcta
     6001 agatgattgt caatggagat atcagtcaga ttgacctgcc acgtaatgtc aagtccggtt
     6061 tgattgatgc tcaagagaaa ctcaagaaca tccatcagat tgactttgtt catttttcag
     6121 ccaaggatgt ggttcgccat ccagttgtcg ctcagattat ccgagcctat gaatattcta
     6181 ctgaagttgc acacgactga ttttgaggaa gttcgcctgc aaaagaatat acttttgaga
     6241 gaggaaaatc cagttgtata ggctaaaggt tttatccaaa ggtttgagac aacgattagg
     6301 cacgatggaa agaactttta tgtggctgat gacgatcagt gcatcttcct gtgtcataat
     6361 cacagggcac aagaaagtag gaatttgaaa agatgattga ccaactatct aagtattaca
     6421 gttgtaggat actaactgaa aaggatattc caagtatttt atctttatat gaaagtaatc
     6481 ctctgtattt tcagcattgt ccaccagagc caaattttgc aactgtaaaa gaggacatgc
     6541 tttgtctacc tgaaggtaaa gctaaggctg ataagttttt ttgttggatt ttggaatgga
     6601 tctgaccttg tggctgttat ggattttgtc tatgcatatc ctgatgagga gactgttttt
     6661 attggtttgt ttatggttga tcaagcctat cagaggaaag ggattggtag tcatattgtg
     6721 acagaagcac tagcttattt tgctaagaac tttcgaaagg cacgtttggc ttatgttaag
     6781 ggaaatccgc aatctcagca tttttgggaa aagcagggtt ttaaatcaat tggatgcgag
     6841 gttaagcaag aactctatac ggttgttatc gctgaacaga gcctagaaga ttagaaatgg
     6901 catcaagtaa gaactatttg gaatttgttt tggaacaatt atcaggatta gatgatgtga
     6961 cttaccgttc catgatgggg gagtatattc tttacttccg cggcaagatt attggcggca
     7021 tttatgatga tcgcttttta gttaaacccg tgcaagcagt cttagataag attgaccaat
     7081 cttcttttga gtttccatac aaaggtgcca aagaaatgat ttgagtggaa gaacttgata
     7141 ataagatgtt tctataagac ctgattttag ctatgtataa ccaactgcca acgcccaaac
     7201 ctaaaaagaa aaagcaaggg tgaacgaagt aaaaaagaag tctgctaagg ccctgtcttt
     7261 gcacgggtaa aattttatat ataaaaagaa gctgggacta aagagctcag cttcctttgg
     7321 tttatataat tgtcattaca agacgaagtg gttgggcgaa actctgttga ctttattcaa
     7381 tttagagttt cttatgcaca attgagtctg gaacgaaagt ctccagttgc aaagtataca
     7441 gtacaataaa ccaacgatgt aatagctgat gacacaaagc acagtgggta ggacttgcga
     7501 agtcaccctt ttcttttcaa aatttatact aaatcattga tatcagtgta gtcacgatta
     7561 agtccttgag caactggtag gctagtcaag taaccttgat aagtggtcac accttgacgc
     7621 aagccttcat cttcagagat tgcttgtgcg aatcctttgc cagccaaagc ttcgatataa
     7681 ggaagagtga cattggttag ggcgatggtt gaagtgcggg caaccgcacc agggatattg
     7741 gcaacggcat agtggagaac accgtgtttt tcatagacgg gttcatcgtg cgttgtcaca
     7801 cggtcagctg tttcgataac gccaccttgg tcaacagcaa cgtcaacgat acagagcctg
     7861 gacgcatttg tttgaccatc tcatctgtca ccaattccgg tgcttttgca ccagggatga
     7921 gaatggctcc aatcaccaca tcagcatctc tcacacttgc ttcaatgttg aatgaattag
     7981 atataagagt ttgaatttga cttccaaaga cttcttctag aactgagaga cgcttggcac
     8041 taatatctaa aatagtcact tgagcaccaa gaccaagggc gatgcgggca gcatgtgtac
     8101 cgacgacacc atcaccgatg atagttactt ttcttttgga acacctggta caccaccagg
     8161 tagaacacca gagccaccag cttgcttagt aaggaagtga gctccgattt aaacagccat
     8221 acgacctgca acctcactca taggaacgag gagcggtagt tgtccttgat tgtcacgaac
     8281 agtttcatag gcaattcctg ttgtttttgc tgctaacata gcatctgcta attctggagc
     8341 agcggccatg tgcaagtagg tgaagagaag aagatcgtcg cgcaagtaac cgtattcaga
     8401 atttaaaggt tcttttactt tcacaaccaa ctctgctgcc caagcttcac cagcagtagc
     8461 gacaatctca gctccttgct tttgatagtc agcatcagta aagccagaac cgagaccagc
     8521 atttgtttcg ataaggacac gatgaccacg actaactaag ctatgaacac ctgcaggtgt
     8581 gagggcgaca cggttttcgt tatttttaat ttcttttggg attccgatta acattgagat
     8641 aacctacctt tcaattgacg gtcttgtttt ggttgtcaca ttccagttca taaatcaaaa
     8701 atgtgacggt ttcattgtat atgaaaccgc ttcaaaaatc aagaaaaact tgtcatccaa
     8761 atttttttat gctagactag tgaaaatcaa gctctaatgg agggaaaagt atggaatcaa
     8821 tttttgtaaa acttgcccag tatccgtcta tagaaacgga gcgtttattg cttagacctg
     8881 taactttgga tgatgcggaa gcaatgtttg actatgcctc ggacaagggt aatacacgtt
     8941 acacttttcc aaccaatcaa agtttggaag aaaccaagaa taacattgct cagttctact
     9001 tggctaatcc cttgggacgt tgggggattg aattaaaaga taacggcaag tttatcggaa
     9061 ctattgactt acacaagatt gatcctgttc ttaagaaggc agctattggc tacattatca
     9121 ataaaaagta ttggaatcaa ggattaacga cagaagccaa tcgtgctgtg attgagctag
     9181 cttttgagaa gatagggatg aataagttga ctgcccttca cgataaggac aatcctgcgt
     9241 caggaaaggt catggagaaa tcaggcatgc gtttttccca tgcagaacca tatgcttgta
     9301 tggaccagca tgaaaaaggc cgaatcgtga caagagttca ttatgtcttg accaaggaag
     9361 actattttgc aaataaataa gcagttgaaa agaaattttt cgactgtttt ttcttcctct
     9421 tacgaataat ctaagagagg agaaaatatg gaagcaatta tcgagaaaat caaagagtat
     9481 aaaatcatcg tcatctgtac tggtctgggc ttgcttgtag gaggattttt cctgctaaaa
     9541 ccagctccac aaacacctgt caaagagacg aatttgcagg ctgaagtcgc agctgtttcc
     9601 aaggatttgg tatccgaaga ggaagtgaac aaggaagaaa aggaagaacc ccttgaacaa
     9661 gatctaatca cagtagatgt caaaggtgct gtcaaatcgc cagggattta tgacttgcct
     9721 gtaggtagtc gagtcaatga tgctgttcag aaggctggtg gcttgacaga gcaagcagac
     9781 agcaagtcgc tcaatctagc tcagaaagtt agtgatgagg ctctggttta cgttcctact
     9841 aagggagaag aagcagttag ccaacagact ggtttgggga cagcttcttc aataagcaag
     9901 gaaaagaagg tcaatctcaa caaggccagt ctggaagaac tcaagcaggt caagggactg
     9961 ggaggaaaac gagctcagga cattatcgac catcgtgagg caaatggcaa gttcaagtca
    10021 gtagacgagc tcaagaaggt ctctggcatt ggtggcaaaa caatagaaaa gcttaaagac
    10081 tatgttacag tggattaaga atttctctat tcccctaatt tacctgagtt ttctattact
    10141 ttggctttat tacgctattt tctcagcatc ttatcttgct ttgttgggct ttgtttttct
    10201 gctagtctgt ctctttatcc aatttccgtg gaaatctgct ggtaaagttc taataatttg
    10261 cggaatcttt ggattttggt ttgtttttca aaattggcaa cagagtcaag cgagtcaaaa
    10321 tctggcggat tctgttgaaa gggtacggat tttacctgat actattaagg ttaatggtga
    10381 tagtctatcc tttcgtggca agtctaacgg tcgtgctttc caagtctatt ataaactcca
    10441 gtccgaggag gagaaagaag cctttcaagc tttaaccgac ctgcatgaga taggactaga
    10501 agggaagctt tcggagccag aagggcagag aaattttggt ggctttaatt accaagccta
    10561 tctgaagact cagggaattt accagactct caatatcaaa aaaatccagt cacttcaaaa
    10621 gattggcagt tgggatatag gagaaaactt gtccagttta cgtcgaaagg ctgtggtttg
    10681 gattaagacg cactttccag accctatgcg caattacatg acaggactct tgctgggata
    10741 tctgaacacc gactttgagg agatgaatga gctttattcc agtctaggaa ttatccacct
    10801 ctttgcccta tctggcatgc aggtaggttt tttcatgaat ggatttaaga aacttctctt
    10861 gcgattgggc ttgacccaag aaaagttgaa atggctgact tatccctttt cccttatcta
    10921 tgcgggacta attggatttt cagcatcggt tattcgcagt ctcttgcaaa agctactggc
    10981 tcaacatggg gttaagggct tggataattt tgccttgacg gtgcttgtcc tctttattgt
    11041 catgccaaac tttttcttga cagcaggagg agtcttgtcc tgcgcttatg cttttatcct
    11101 gaccatgacc agcaaagaag gggaggggct caaggctgtt gctagtgaaa gtctagtcat
    11161 ctccttgggc atattgccca ttctatcctt ctattttgcg gaatttcaac cttggtctat
    11221 ccttttgacc tttgtctttt cctttctatt tgacttgacc ttcttaccgc tcttgtctat
    11281 tttatttgtc ctttcctttc tctatccagt cattcagctg aactttatct ttgaatggtt
    11341 agagggcatt attcgcttgg tctcgcaggt ggcaaggaga ccgcttgtct ttggtcaacc
    11401 caacgcatgg cttttaatct tattgttaat ttccttggct ttggtctatg atttgaggaa
    11461 aaacattaaa ggattaacag tattgagttt attgattaca ggtctctttt tccttaccaa
    11521 gtatccactg gaaaatgaaa tcaccatgct ggatgtgggg caaggagaaa gtattttcct
    11581 acgggatgta actgggaaaa ccattctcat agatgtaggt ggtaaggcag aatcttataa
    11641 gaaaatcgaa aaatggcaag aaaagatgac gaccagcaat gcacagagaa gcttgatacc
    11701 ctatcttaaa agtcgaggag tagctaagat tgaccagctg attttgacaa atacggacaa
    11761 ggaacatgtc ggagatttgt tagaggtgac caaggctttc catgtagggg agattttagt
    11821 atcaaaaggc agtttgaagc agaagcaatt tgtggtagaa ctacaggcga ctcaaacaaa
    11881 ggtgcgtagt atgacagtag gggagaactt gcccattttt ggaagtcagt tagaagttct
    11941 atccccaagg aaaatgggag atggaggaca cgatgatacc ctagttctgt atgggaaatt
    12001 cttggataag caatttctct tcacgggaaa tttggaggag aaaggagaga aggacttgct
    12061 gaagcactat ccagacttga aagtaaatgt tttgaaagct agccaacatg gcaataaaaa
    12121 atcatcaagt ccagcctttc tagaaaaact caaaccaaag cttactctta tctcagttgg
    12181 aaagagcaat cgaatgaaac tcccccatca ggaaacattg acacgactgg aaggtatcaa
    12241 tagcaaagtt taccgaactg accagcaagg agctatacgg tttaaaggtt ggaatagttg
    12301 gaaaatcgaa agtgttcgat aggaaagata aatgttgtag attagtgaaa taaactaaaa
    12361 atttgttgca taataatgat aaaaatggta taatgaaaac gtattcaata ctgaggatat
    12421 aaaatcatta aaaattagca aaaatcgttt aattagttag tttatgatta ttggtctttt
    12481 tcagtccagt atatctgctg tgacagtcac taaaagttac aagtatgatt ggaatacggt
    12541 ttgggaatat agtactaact atcacgacca tcagtatgct tggattccgt catggtctcg
    12601 ttattacagc tattctgagt atccagttgg ttctggctgg aactacgctc gttatgaggt
    12661 cataaactac tatactggag gttattaatc cttaaagagt gagaaaagga gggctggata
    12721 tgttaaggct tactcatgtt accctaaaaa cgcgacaagt catcttgcaa gatgcggatt
    12781 ttacctttaa aaagggtagg gtttatggtc ttcttgctat caatggctcg ggaaagacga
    12841 cactattccg agctatgagc aagttgcttc cccttagtag tggacacatc gcagttcctc
    12901 cttctttgtt ttattatgag agcgttgaat ggctggatgg aaacttaagt gggatggact
    12961 accttcgtct cataaaaaac atctggaagt cagacctaaa cttgagagat gaaatcgcct
    13021 actgggaaat ggctgactat atcagtcttc ccatccgcaa gtattcctta ggcatgaagc
    13081 aacgcttggt gattgctatg tattttctca gtcaggccaa atgctggctc atggatgaga
    13141 ttacaaatgg cttagacgag tattatcgac agaagttttt tgataggcta gcacaaatcg
    13201 atagacaaga acagctggtt cttttaagtt cccactataa ggaagagttg gttgatatct
    13261 gcgatagagt agtaaccatt catcaggggc agatagaaga ggtttagttt atgaaagatg
    13321 ttagtctatt tttattgaaa aaagttttca aaagtcgttt aaactggatt atcttacttt
    13381 tatttgcatc tgtactcggt gttacctttt atttaaatag tcagactgca aactcacaca
    13441 gcttggagag caggttggaa agtcgcattg cagccaacga gagggctatc aatgaaaatg
    13501 aagagaaact ctcccaaatg tctgatacca gctcggagga ataccagttt gctaaaaata
    13561 atttagacgt gcaaaaaaat cttttgacgc gaaagacaga aattctaact ttactaaaag
    13621 aagggcgctg gaaagaagcc tactatctgc agtggcaaga tgaagagaag aattatgaat
    13681 ttgtatcaaa tgacccgact gctagctctg gcttaaaaat gggggttgac cgcgaacgga
    13741 agatttacca agccctgtat cccttgaaca taaaagcaca tactttggag tttccgaccc
    13801 acgggattga tcagattgtc tggattttag aggttatcat cccaagcttg tttgtggttg
    13861 cgattatttt tatgctaaca caactatttg cagaaagata tcaaaatcat ctggacacag
    13921 ctcacttata tcctgtttca aaagtgacat ttgcaatatc ctctcttgga gttggagtgg
    13981 gatatgtaac tgtgctgttt atcggaatct gtggcttttc ttttctagtg ggaagtctga
    14041 taagtggttt tggacagtta gattatccct acccaattta tagcttagtg aatcaagaag
    14101 taactattgg gaaaatacaa gatgtattat ttcctggctt gctcttagct ttcttagcct
    14161 ttatcgtcat tgtggaagtc gtctacttga ttgcttactt tttcaagcaa aaaatgcctg
    14221 tcctctttct ttcactcatt gggattgttg gcttattgtt tggcatccaa acaattcagc
    14281 ctcttcaaag gattgcacat ctgattccct ttacttactt gcgttcagtg gagattttat
    14341 ctggaagatt atctaagcag attgataatg tcgatctaaa ttggagcatg gggatggtct
    14401 tacttccttg cctgattatc cttttgttag tggggattct atttatcgaa agctggggaa
    14461 gttcacgtaa aaaggaagtt tttaaagttt agttttccta tagggaagct aaagaaaaac
    14521 taacataggg agagaatcaa cttggttctc tctttttgat tcgaaaacca aatcaaaata
    14581 caaacacaaa cttttcaaaa aataactttt tatcttgaca agagctagaa aacttggtat
    14641 catataaaag ttgagaaaag cagaagtgag agcttctcgc cttgtgacat taagttgcct
    14701 ggccctacgg atgaaaagtt tcgaagaaac gctatcataa cgtgcgggct tgtatattta
    14761 caagtccgct attgtttttc tctaataaaa caaaagaggt gaaaaccata gcaaagcaag
    14821 acttattcat caatgatgag attcgtgtac gtgaagttcg cttgattggt cttgaaggag
    14881 aacagctagg tatcaagcca ctcagtgaag cgcaagcttt ggctgataac gctaatgttg
    14941 acctagtatt gattcaaccc caagccaaac cgcctgttgc aaaaattatg gactacggta
    15001 agttcaaatt tgagtaccag aagaagcaaa aagaacaacg taaaaaacaa agcgttgtta
    15061 ctgtgaaaga agttcgtcta agtccaacta ttgacaaggg tgactttgat acaaaacttc
    15121 gtaatgcacg taagttcctt gaaaaaggaa ataaagttaa ggtatctatt cgctttaagg
    15181 gtcgtatgat tacccataaa gagattggtg caaaagtttt agccgagttt gctgaagcaa
    15241 ctcaagatat tgccatcatc gaacaacgtg ctaaaatgga tggacgtcaa atgttcatgc
    15301 aattggcgcc agcgactgac aaaaaataat tgtcagaaag ttaaataaag gagaaaaaat
    15361 catgccaaaa caaaaaacac accgcgcatc agctaaacgt ttcaaacgta caggttctgg
    15421 tggacttaaa cgtttccgtg cttacacttc tcaccgtttc cacggaaaaa ctaagaaaca
    15481 acgtcgtcat cttcgtaaag catctatggt gcattcagga gattacaaac gtatcaaagc
    15541 aatgcttact cgcttgaaat aatagcttga ctgtaagtaa acaattctag gaaatatttg
    15601 gaggaaataa aacatggcac gtgttaaagg tggcgttgta tcacgcaaac gtcgtaaacg
    15661 tattcttaaa ttagcaaaag gttactatgg agctaaacac atcttgttcc gtactgcaaa
    15721 agaacaagta atgaactctt actactatgc ataccgtgac cgtcgtcaga aaaaacgtga
    15781 cttccgcaaa ttgtggatta ctcgtatcaa tgcggcggct cgtatgaacg gactttcata
    15841 ctcacaattg atgcatggtt tgaaattggc tgagatcgaa gttaaccgta aaatgcttgc
    15901 tgacttggct gttaacgatg cagtagcttt cacagctctt gcagatgcag ctaaagcaaa
    15961 acttggtaaa taacaactga tgagactgga acaggattgt ccagtctttt taaaaataaa
    16021 ggagaaagat atggcttcaa aaatgctaca cacttgctta cgagtagaaa atcttgaaaa
    16081 atcaatcgca ttttatcaag atgcttttgg ttttaaagaa ttgcgtcgca gagattttcc
    16141 agaccatgcc ttcacgattg tctatctagg tcttgagggt gatgattatg agttggagtt
    16201 gacttataac tacgatcacg gtccgtatgt ggttggtgat gggtttgccc atatcgccct
    16261 cagtacacct gatcttgagg cacttcatca agaacacagt gcaaaaggct atgaagtgac
    16321 tgagccaaat ggtctaccag gaactacacc aaactattac tttgttaaag accctgatgg
    16381 atataaggtc gaagtcattc gtgaaaaata atttcgtgca agttaagtag aatattcgtt
    16441 ttctacttga ctttttttag ctgaaagagt atactaataa aaattcaacc tttaagggga
    16501 gtccagagag actcacaagg tgtcagataa aagaatagtg caattttcta gaggagacct
    16561 tttgagtgtg ctctcttgtg ttgtacgatt ttaactgagg ccttgcacta gcaaggtctt
    16621 ttctttatct ggtcccctta aaatttaagg aggaaaagtt atgaatctca catgtaagaa
    16681 gcgtttgggt gtcattcggt tggaaaccat gaaggtggtt gcacaagagg aaatcgcgcc
    16741 agcaatcttt gaattagtcc tagaaggaga aatggttgaa gccatgcgag caggccaatt
    16801 tcttcatctg cgtgtaccag acgatgccca tctcttacgt cgtcctattt caatttcgtc
    16861 tattgacaag gcaaacaagc agtgtcacct catttatcgg attgagggag ctgggactgc
    16921 aattttttca accttaagtc agggagacac tcttgatgtg atgggtcctc agggaaatgg
    16981 ttttgacttg tctgaccttg atgagcagaa tcaggttctc cttgttggtg gtgggattgg
    17041 tgttccaccc ttgcttgagg tggccaagga attgcatgaa cgtggagtga aagtagtgac
    17101 agtcctcggt tttgctaata aggatgctgt tattttgaaa acggaattgg ctcagtatgg
    17161 tcaggtcttt gtaacgacag atgatggttc ttatggcatc aagggaaatg tttccgttgt
    17221 tatcaatgat ttagatagtc agtttgacgc tgtttactcg tgtggggctc caggaatgat
    17281 gaagtatatc aatcaaacct ttgatgatca cccaagagcc tatttatctc tggaatctcg
    17341 tatggcttgt gggatgggag cttgctatgc ctgtgttcta aaagtaccag aaagcgagac
    17401 ggtcagccaa cgcgtctgtg aagatggtcc tgttttccgc acaggaacag ttgtattata
    17461 aggagaaaat tatgactaca aatcgattac aagtgtctct acctggtttg gatttgaaaa
    17521 atccgattat tccagcatca ggctgttttg gctttggaca agagtatgcc aagtactatg
    17581 atttaaacct tttaggttct attatgatca aggcgacaac ccttgaacca cgttttggga
    17641 acccaactcc aagagtagca gagacgcctg ctggtatgct caatgcaatt ggcttgcaaa
    17701 atcctggttt agaggttgtt ttggctgaaa agttaccttg gctggaaaga gaatatccaa
    17761 atcttcctat tattgccaat gtagctggtt tttcaaaaca agagtatgca gctgtttctc
    17821 atgggatttc caaggcaact aatgtaaaag ctatcgagct caatatttct tgtcccaatg
    17881 ttgaccactg taatcatgga cttttgattg gtcaagatcc agatttggct tatgatgtgg
    17941 tgaaagcagc tgtggaagcc tcagaagtgc cagtttatgt caaattaacc ccgagtgtga
    18001 ccgatatcgt tactgtcgca aaagctgcag aagatgcggg agcaagtggc ttgaccatga
    18061 tcaatactct ggttggaatg cgctttgacc tcaaaaccag aaaaccaatc ttggccaatg
    18121 gaacaggtgg aatgtcaggt ccagcagtct ttccagtagc cctcaaactc atccgccaag
    18181 tagcccaaac aacagacctg cctatcattg gaatgggggg agtggattcg gctgaagctg
    18241 ccctagaaat gtatctggct ggggcatctg ctatcggagt tggaacagct aactttacca
    18301 atccttatgc ctgccctgac atcatcgaaa atttaccaaa agtcatggat aaatacggta
    18361 ttagcagtct ggaagaactc cgtcaggaag taaaagagtc tctgaggtaa actgcaatca
    18421 atctgttctt gattttttat tagtttgtaa tatgaattta ggagaatttt ggtacaataa
    18481 aataaataag aacagaggaa gaaggttaat gaagaaagta agatttattt ttttagctct
    18541 gctatttttc ttagctagtc cagagggtgc aatgactagt gatggtactt ggcaaggaaa
    18601 acagtatctg aaagaagatg gcagtcaagc agcgaatgag tgggtttttg atactcatta
    18661 tcaatcttgg ttctatataa aagcagatgc taactatgct gaaaatgaat ggctaaagca
    18721 aggtgacgac tatttttacc tcaaatctgg tggctatatg gccaaatcag aatgggtaga
    18781 agacaaggga gccttttatt atcttgacca agatggaaag atgaaacgaa atgcttgggt
    18841 aggaacttcc tatgttggtg caacaggtgc caaagtaata gaagactggg tctatgattc
    18901 tcaatacgat gcttggtttt atatcaaagc agatggacag cacgcagaga aagaatggct
    18961 ccaaattaaa gggaaggact attatttcaa atccggtggt tatctactga caagtcagtg
    19021 gattaatcaa gcttatgtga atgctagtgg tgccaaagta cagcaaggtt ggctttttga
    19081 caaacaatac caatcttggt tttacatcaa agaaaatgga aactatgctg ataaagaatg
    19141 gattttcgag aatggtcact attattatct aaaatccggt ggctacatgg cagccaatga
    19201 atggatttgg gataaggaat cttggtttta cctcaagttt gatggcaaaa tggctgaaaa
    19261 agaatgggtc tacgattctc atagtcaagc ctggtactac ttcaaatccg gtggttacat
    19321 gacagccaat gaatggattt gggataagga atcttggttt tatctcaaat ctgatgggaa
    19381 aatagctgaa aaagaatggg tctacgattc tcatagtcaa gcttggtact acttcaaatc
    19441 cggtggttac atgacagcca atgaatggat ttgggataag gaatcttggt tttacctcaa
    19501 gtctgatggc aaaatggctg aaaaagaatg ggtctacgat tctcatagtc aagcctggta
    19561 ctacttcaaa tctggtggct acatggcgaa aaatgagaca gtagatggtt atcagcttgg
    19621 aagcgatggt aaatggcttg gaggaaaagc tacaaataaa aatgctgctt actatcaagt
    19681 agtgcctgtc acagccaatg tttatgattc agatggtgaa aagctttcct atatatcgca
    19741 aggtagtgtc gtatggctag ataaggatag aaaaagtgat gacaagcgct tggctattac
    19801 tatttctggt ttgtcaggct atatgaaaac agaagattta caagcgctag atgctagtaa
    19861 ggactttatc ccttattatg agagtgatgg ccaccgtttt tatcactatg tggctcagaa
    19921 tgctagtatc ccagtagctt ctcatctttc tgatatggaa gtaggcaaga aatattattc
    19981 ggcagatggc ctgcattttg atggttttaa gcttgagaat cccttccttt tcaaagattt
    20041 aacagaggct acaaactaca gtgctgaaga attggataag gtatttagtt tgctaaacat
    20101 taataatagc cttttggaga acaagggcgc tacttttaag gaagccgaag aacattacca
    20161 tatcaatgcc ctttatctcc ttgcccatag tgccctagaa agtaactggg gaagaagtaa
    20221 aattgccaaa gataagaata atttctttgg cattacagcc tatgatacga ccccttacct
    20281 ttctgccaag acatttgatg atgtggataa gggaatttta ggtgcaacca agtggattaa
    20341 ggaaaattat atcgataggg gaagaacttt ccttggaaac aaggcttctg gtatgaatgt
    20401 ggaatatgct tcagaccctt attggggcga aaaaattgct agtgtgatga tgaaaatcaa
    20461 tgagaagcta ggtggcaaag attagtacta taagtgaata tgatttgagt gaatagtaag
    20521 ttaaaaatcc tgatttcaag taaaatcagg attttttcat ggatgcaatt tttttggagt
    20581 ctggtgtgac gcggagggtc ttttgtcctg tgtaagtgac aaagccgggt tttccaccag
    20641 ttggtttatt gagttttttg acttcaatca tatctacctg caccagattc gacaggcgcc
    20701 cttgagagaa gtaggcagct aactctgctg cgtctgtctt gactgcatca gatgggtcaa
    20761 gatttcctga gatgacaaca tggcttccag gaatgtcctt agcatggaac caaagttcct
    20821 ccttgcgggc cattttaaag gtcaattcct cattttgaag attgtttcgt ccgacataga
    20881 tgatggtttt gccatcgctt gctagatatt gttctagttt tttgcgtttc tggattttct
    20941 cccgttgtct tctgcggata aaacctgttt gaatcaattc ttcacggatt tcagcgattt
    21001 cttccagtcc agcttggttg aggacggttt ctacactttc cagatagaga atagtggctt
    21061 tggtttcttc aatcaaatca gtcaagtatt tgacagcttc tttgagtttc tgataccgtt
    21121 taaaatagcg ttgggcattc tggttgggag tcagagcctt atcaagcgca atcatgatag
    21181 gttggttggt atagtagttg tctaggataa cctggtcttg gtcgttaggc acttggtgga
    21241 ggaaggttgt cagcaattct cctttttgac gaaattcttc agcgttgtct gtcgccagta
    21301 actctttttc ctgttttttg agtttgtgtc ggtttttctg aagttcattt tcaacacgac
    21361 gaatcagttc actggcctgc tgtttgacgc ggtcgcgctc agccttatcc ttatagtagg
    21421 tgtccaacaa atcagaaaga tttgcaaaag gctctcccac ctgatttgca aaaggaactg
    21481 gactgaagga agtctcagtc aagcatggct tggtttcctg attgaaaaaa tttcggaaag
    21541 cggaaagttt ttcactaacc agtatccttt ccaattcatt tgccgtatcg cgtcccagac
    21601 cttgaaagag gctttgaaga ttttttgctg ttagttcttg ggtttgcagg atttcaaaga
    21661 gcttttcatc cttgatagta aaaggattga gagattctgt acttggcgga gcgatatagg
    21721 tcgatcctgg aagtaaggtg cggtagctat tttgtgaaaa accgacgtgt ttgataactt
    21781 cgaggatttt atgactgctt ttatcgacca gtagaatatt actgtgtttc cccataattt
    21841 cgataatcaa ggtagcctgg atatggtctc caatctcgtt tttattggaa actgtcattt
    21901 ccacaatacg gtcattttcc acttgctcaa tcgactcaat cagggccccc tgcaaatact
    21961 ttctcaaaac catgataaag gtagaaggtt gggctggatt ttcaaaagtc gtttgggtca
    22021 gctgaatgcg tccaaaaact ggatgggcag aaaggagcag gcgatggctt tggcgattgc
    22081 tgcggatttg caagaccaac tcttgttcaa aaggctgatt gattttctgg atgcgaccat
    22141 tcactaattc gcttcgcaat tcctcaacta tgtggtgtaa aaaaaatccg tcaaatgaca
    22201 tcgttctctc cttgtgattg tattccatag tattatatca aaaaggtaga ataaaatcat
    22261 ggaaatgtgg tataataaag ccaagtaaag agaaacgaga agcacatgta tattgaaatg
    22321 gtagatgaaa ctggtcaagt ttcaaaagaa atgttgcaac aaacccaaga aattttggaa
    22381 tttgcagccc aaaaattagg aaaagaagac aaggagatgg cagtcacttt tgtgaccaat
    22441 gagcgtagtc atgaacttaa tctggagtac cgtgacaccg accgtccgac agatgtcatc
    22501 agccttgagt ataaaccaga attggaaatt gcctttgacg aagaggattt gcttgaaaat
    22561 ccagaattgg cagagatgat gtctgagttt gatgcctata ttggggaatt gttcatctct
    22621 atcgataagg ctcatgagca ggccgaagaa tatggtcaca gctttgagcg tgagatgggc
    22681 ttcttggcag tacacggctt tttacatatt aacggctatg atcactacac tccggaagaa
    22741 gaagcggaga tgttcggttt acaagaagaa attttgacag cctatggact cacaagacaa
    22801 taaacgaaaa tggaaaaatc gtgacctgat atccagttta gaatttgcca tcacagggat
    22861 ttttactgct atcaaggaag aacgaaatat gcgaaaacat gcagtgacgg ctctagtggt
    22921 tatccttgca ggttttgttt ttcaggtgtc acgaatcgaa tggctctttc tcctattgag
    22981 cattttcttg gtagtagcct ttgagattat caactctgct attgaaaata tggtggattt
    23041 ggccagtcac tatcactttt ccatgctggc taaaaatgcc aaggatatgg cggctggcgc
    23101 ggtattagtg gtttctcttt tcgcagcctt aacaggtgca ttgatttttc tcccacgaat
    23161 ctgggattta ttattttaaa cagtaagagg aaattatgac ttttaaatca ggctttgtag
    23221 ccattttagg acgtcccaat gttgggaagt caaccttttt aaatcacgtt atggggcaaa
    23281 agattgccat catgagtgac aaggcgcaga caacgcgcaa taaaatcatg ggaatttaca
    23341 cgactgataa ggagcaaatt gtctttatcg acacaccagg gattcacaaa cctaaaacag
    23401 ctctcggaga tttcatggtt gagtctgcct acagtaccct tcgcgaagtg gacaccgttc
    23461 ttttcatggt gcctgctgat gaagcgcgtg gtaaggggga cgatatgatt atcgagcgtc
    23521 tcaaggctgc caaggttcct gtgattttgg tggtgaataa aatcgataag gtccatccag
    23581 accagctctt gtctcagatt gatgacttcc gtaatcaaat ggactttaag gaaattgttc
    23641 caatctcagc ccttcaggga aataacgtgt ctcgtctagt ggatattttg agtgaaaatc
    23701 tggatgaagg tttccaatat ttcccgtctg atcaaatcac agaccatcca gaacgtttct
    23761 tggtttcaga aatggttcgc gagaaagtct tgcatctaac tcgtgaagag attccgcatt
    23821 ctgtagcagt agttgttgac tctatgaaac gagacgaaga gacagacaag gttcacatcc
    23881 gtgcaaccat catggttgag cgcgatagcc aaaaagggat tatcatcggt aaaggtggcg
    23941 ctatgcttaa gaaaatcggt agcatggccc gtcgtgatat cgaactcatg ctaggagaca
    24001 aggtcttcct agaaacctgg gtcaaggtca agaaaaactg gcgcgataaa aagctagatt
    24061 tggctgactt tggctataat gaaagagaat actaagtaga ggtaggctca tgcctgcttc
    24121 ttgtttttac agaaggagac ttatgcctga attacctgag gttgaaaccg tttgtcgtgg
    24181 cttagaaaaa ttgattatag gaaagaagat ttcgagtata gaaattcgct accccaagat
    24241 gattaagacg gatttggaag agtttcaaag ggaattgcct agtcagatta tcgagtcaat
    24301 gggacgtcgt ggaaaatatt tgctttttta tctgacagac aaggtcttga tttcccattt
    24361 gcggatggag ggcaagtatt tttactatcc agaccaagga cctgaacgca agcatgccca
    24421 tgttttcttt cattttgaag atggtggcac gcttgtttat gaggatgttc gcaagtttgg
    24481 aaccatggaa ctcttggtgc ctgacctttt agacgcctac tttatttcta aaaaattagg
    24541 tcctgaacca agcgaacaag attttgattt acaggtcttt caatctgccc ttgccaagtc
    24601 caaaaagcct atcaaatccc atctcctaga ccagaccttg gtagctggac ttggcaatat
    24661 ctatgtggat gaggttctct ggcgagctca ggttcatcca gctagacctt cccagacttt
    24721 gacagcagaa gaagcgactg ccattcatga ccagaccatt gctgttttgg gccaggctgt
    24781 tgaaaaaggt ggctccacca ttcggactta taccaatgcc tttggggaag atggaagcat
    24841 gcaggacttt catcaggtct atgataagac tggtcaagaa tgtgtacgct gtggtaccat
    24901 cattgagaaa attcaactag gcggacgtgg aacccacttt tgtccaaact gtcaaaggag
    24961 ggactgatgg gaaaaatcat cggaatcact gggggaattg cctcaggtaa gtcaactgtg
    25021 acaaattttc taagacagca aggctttcaa gcagtggatg ccgacgcagt cgttcaccaa
    25081 ctacagaaac ctggtggtcg tctgtttgag gctatagtac agcactttgg gcaagaaatc
    25141 attcttgaaa acggagaact caatcgccct ctcctagcta gtctcatctt ttcaaatcct
    25201 gaagagcaaa aatggtctaa tcaaattcaa ggggagatta tccgtgagga actggctact
    25261 ttgagagaac aattggctca gacagaagag attttcttca tggatattcc cctacttttt
    25321 gagcaggact acagcgattg gtttgctgag acttggttgg tctatgtgga ccgagatgcc
    25381 caagtagaac gcttaatgaa aagggaccag ttgtccaaag atgaagctga gtctcgtctg
    25441 gcagcccagt ggcctttaga aaaaaagaaa gatttggcca gccaggttct tgataataat
    25501 ggcaatcaga accagcttct taatcaagtg catatccttc ttgagggagg taggcaagat
    25561 gacagagatt aactggaagg ataatctgcg tattgcctgg tttggtaatt ttctgacagg
    25621 agccagtatt tctttggttg taccttttat gcccatcttc gtggaaaatc taggtgtagg
    25681 gagtcagcaa gtcgcttttt atgcaggctt agcaatttct gtctctgcta tttccgcggc
    25741 gctcttttct cctatttggg gtattcttgc tgacaaatac ggccgaaaac ccatgatgat
    25801 tcgggcaggt cttgctatga ctatcactat gggaggcttg gcctttgtcc caaatatcta
    25861 ttggttaatc tttcttcgtt tactaaacgg tgtatttgca ggttttgttc ctaatgcaac
    25921 ggcactgata gccagtcagg ttccaaagga gaaatcaggc tctgccttag gtactttgtc
    25981 tacaggcgta gttgcaggta ctctaactgg tccctttatt ggtggcttta tcgcagaatt
    26041 atttggcatt cgtacagttt tcttactggt tggtagtttt ctatttttag ctgctatttt
    26101 gactatttgc tttatcaagg aagattttca accagtagcc aaggaaaagg ctattccaac
    26161 aaaggaattg tttacttcag ttaaatatcc ctatcttttg ctcaatctct ttttaaccag
    26221 ttttgtcatc caattttcag ctcaatcgat cggccctatt ttggctcttt atgtacgcga
    26281 cttagggcag acagagaatc ttctttttgt ctctggtttg attgtgtcca gtatgggctt
    26341 ttccagcatg atgagtgcag gagtcatggg caagctaggt gacaaggtgg gcaatcatcg
    26401 tctcttggtt gtcgcccagt tttattcagt catcatctat ctcctctgtg ccaatgcctc
    26461 tagccccctt caactaggac tctatcgttt cctctttgga ttgggaaccg gtgccttgat
    26521 tcccggggtt aatgccctac tcagcaaaat gactcccaaa gccggcattt cgaggatctt
    26581 tgccttcaat caggtattct tttatctggg aggtgttgtt ggtcccatgg caggttctgc
    26641 agtagcaggt caatttggct accatgctgt cttttatgcg acaagccttt gtgttgcctt
    26701 tagttgtctc tttaacctga ttcaatttcg aacattatta aaagtaaagg aaatctagtg
    26761 cgagtaaaaa ttaatctcaa atgctcctct tgtggcagta tcaattacct aaccagtaaa
    26821 aattcaaaaa cccatccaga caagattgag gttttaaagt attgtcccaa ggaaagaaaa
    26881 gtaaccctac atcttgaatc taagtagatt ttatggtaaa ataataggga ttttaa
//
LOCUS       DAVLSL010000014        26713 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLSL010000014 DAVLSL010000000
VERSION     DAVLSL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.014"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            145..693
                     /locus_tag="VXN30_000463"
     CDS             145..693
                     /locus_tag="VXN30_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1357134.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            803..1702
                     /locus_tag="VXN30_000464"
     CDS             803..1702
                     /locus_tag="VXN30_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1357135.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTILPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPSFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1727..3001)
                     /gene="serS"
                     /locus_tag="VXN30_000465"
     CDS             complement(1727..3001)
                     /gene="serS"
                     /locus_tag="VXN30_000465"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1357136.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3214..3585)
                     /locus_tag="VXN30_000466"
     CDS             complement(3214..3585)
                     /locus_tag="VXN30_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1357137.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3588..4952)
                     /locus_tag="VXN30_000467"
     CDS             complement(3588..4952)
                     /locus_tag="VXN30_000467"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1357138.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5277..6062
                     /locus_tag="VXN30_000468"
     CDS             5277..6062
                     /locus_tag="VXN30_000468"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1357139.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6156..6590
                     /locus_tag="VXN30_000469"
     CDS             6156..6590
                     /locus_tag="VXN30_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1357140.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6590..7564
                     /locus_tag="VXN30_000470"
     CDS             6590..7564
                     /locus_tag="VXN30_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1357141.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7624..7848
                     /locus_tag="VXN30_000471"
     CDS             7624..7848
                     /locus_tag="VXN30_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1357142.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7967..8941
                     /gene="fabK"
                     /locus_tag="VXN30_000472"
     CDS             7967..8941
                     /gene="fabK"
                     /locus_tag="VXN30_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1357143.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            8934..9854
                     /gene="fabD"
                     /locus_tag="VXN30_000473"
     CDS             8934..9854
                     /gene="fabD"
                     /locus_tag="VXN30_000473"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1357144.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSNFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9888..10619
                     /gene="fabG"
                     /locus_tag="VXN30_000474"
     CDS             9888..10619
                     /gene="fabG"
                     /locus_tag="VXN30_000474"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1357145.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            10641..11876
                     /gene="fabF"
                     /locus_tag="VXN30_000475"
     CDS             10641..11876
                     /gene="fabF"
                     /locus_tag="VXN30_000475"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1357146.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEVNVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11879..12364
                     /locus_tag="VXN30_000476"
     CDS             11879..12364
                     /locus_tag="VXN30_000476"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1357147.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12361..12783
                     /gene="fabZ"
                     /locus_tag="VXN30_000477"
     CDS             12361..12783
                     /gene="fabZ"
                     /locus_tag="VXN30_000477"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1357148.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSENTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12795..14162
                     /gene="accC"
                     /locus_tag="VXN30_000478"
     CDS             12795..14162
                     /gene="accC"
                     /locus_tag="VXN30_000478"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1357149.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14199..15065
                     /locus_tag="VXN30_000479"
     CDS             14199..15065
                     /locus_tag="VXN30_000479"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1357150.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15062..15829
                     /locus_tag="VXN30_000480"
     CDS             15062..15829
                     /locus_tag="VXN30_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1357151.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            15987..16172
                     /gene="briC"
                     /locus_tag="VXN30_000481"
     CDS             15987..16172
                     /gene="briC"
                     /locus_tag="VXN30_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1357152.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            16190..16663
                     /locus_tag="VXN30_000482"
     CDS             16190..16663
                     /locus_tag="VXN30_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357153.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            16676..16912
                     /locus_tag="VXN30_000483"
     CDS             16676..16912
                     /locus_tag="VXN30_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1357154.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16959..17381)
                     /gene="nusB"
                     /locus_tag="VXN30_000484"
     CDS             complement(16959..17381)
                     /gene="nusB"
                     /locus_tag="VXN30_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1357155.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17374..17763)
                     /locus_tag="VXN30_000485"
     CDS             complement(17374..17763)
                     /locus_tag="VXN30_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1357156.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(17785..18345)
                     /gene="efp"
                     /locus_tag="VXN30_000486"
     CDS             complement(17785..18345)
                     /gene="efp"
                     /locus_tag="VXN30_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1357157.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(18474..19916)
                     /gene="gatB"
                     /locus_tag="VXN30_000487"
     CDS             complement(18474..19916)
                     /gene="gatB"
                     /locus_tag="VXN30_000487"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1357158.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(19916..21382)
                     /gene="gatA"
                     /locus_tag="VXN30_000488"
     CDS             complement(19916..21382)
                     /gene="gatA"
                     /locus_tag="VXN30_000488"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1357159.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKHSEETIYQVAAAFEATTGYHKQQPVIFGGDN"
     gene            complement(21382..21684)
                     /gene="gatC"
                     /locus_tag="VXN30_000489"
     CDS             complement(21382..21684)
                     /gene="gatC"
                     /locus_tag="VXN30_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1357160.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            21897..23441
                     /locus_tag="VXN30_000490"
     CDS             21897..23441
                     /locus_tag="VXN30_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1357161.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            23695..23892
                     /locus_tag="VXN30_000491"
     CDS             23695..23892
                     /locus_tag="VXN30_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1357162.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            24003..24179
                     /locus_tag="VXN30_000492"
     CDS             24003..24179
                     /locus_tag="VXN30_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1357163.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAISLAAVRDDFYTHNFNSGILLIN
                     NGMWRAENVTQDLI"
     gene            24295..24483
                     /locus_tag="VXN30_000493"
     CDS             24295..24483
                     /locus_tag="VXN30_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1357164.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            24640..25005
                     /locus_tag="VXN30_000494"
     CDS             24640..25005
                     /locus_tag="VXN30_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1357165.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            25008..26675
                     /locus_tag="VXN30_000495"
     CDS             25008..26675
                     /locus_tag="VXN30_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1357166.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         7666 a         5080 c         6172 g         7795 t
ORIGIN      
        1 gcaagtgatt ggaagtgtga taaactaata taacaacgaa aatcttatga gagaataaag
       61 ctttatctta gataaactta ggaatcagga ttttataaga ttttccaata atattagtgt
      121 caataataag aaaagaggta tcttatgaca acatttacta tccatacagt agagtcagca
      181 ccagcagaag tgaaagaaat tcttgaaaca gtagaaaaag acaacaatgg ctatattccc
      241 aacctaatcg gtctcttggc caatgccccg actgttttag aagcctacca aattgtctca
      301 tctatccacc gtcgcaacag cctgacaccc gttgagcgtg aagtggtgca aatcacggca
      361 gccgtgacca atggttgtgc cttctgtgtc gcaggtcaca cagccttttc catcaaacaa
      421 atccagatga atgatgactt gattcaagct cttcgcaatc gtactccaat tgaaacagat
      481 cctaaattgg ataccctagc taagtttacc ttggcagtta tcaataccaa gggtcgtgta
      541 ggagatgaag ccttgtctga gtttttagaa gctggctaca ctcaacaaaa tgccttggat
      601 gtggtttttg gtgtcagcct agcaatcctc tgtaactatg ccaacaactt agctaataca
      661 ccaattaatc cagaattgca accttatgcc taattcatat ctgagtaaaa tgaagtctga
      721 aataagcgga cttcgttttt ttaaatcctc atttaagcgc ttttatgtta tactgaaact
      781 aacatgatta ttggaggtta ggatgaaaaa actcccctta gtattttcag gttgtttgct
      841 aggtttggca ggagctggaa atcttatttt agatacgttg ccggttctat cccatctttt
      901 tagtctgact ggtttggttt tatggattta ctttctaatt ctgcatctct ttaattggaa
      961 agaaaccaag caagaattga ccaagccccc tcttttgtca ggaatggcaa cctttcctat
     1021 ggctgggatg attttatcga cctatgtctt tcgcgtattc tcttatcttc ctttggtagc
     1081 acaagggatt tggtggtttt catttctctt ggatttgacc ttgattgctg gttttaccat
     1141 caagtttgct tgttcagggc ggagggttca tgccactcca agttggacgg ttctctatgt
     1201 ggggatagca gtggctgcct taacctatcc tctggtaggt attatcgaaa ttgcctatgc
     1261 gaccttgagt tttggttttc tcttgacctt ctatctctat ccccttattt atagcgattt
     1321 aaagaaacat ccactcccac tagccttgct tggacaagaa ggaatctact gtgctccttt
     1381 ctctctactc ttggcttctc tagttcgagt aggaggaacc atcctgccga cttgggtctt
     1441 gattgtcatg attttggctt ctcaatcctt ctttttcttt gttttaactc gtctgcccaa
     1501 cattttaaaa caaggttttc aaccatcctt ctcagccctc accttcccaa ccattatcac
     1561 agcgacctcg ctcaagatgg ctcagggaat tttgaaactt ccatttctgg attacctggt
     1621 attggctgaa accattatat gcctaactat tttattcttt gtactaggtg cttatctgat
     1681 ttggttacga aaaaaggtct agctagaaat agctaaacct tattttttat ggtttgatga
     1741 cttcagctcc acccatgtat ggacgaagtg cttctgggat ggtcacagaa ccatcttcat
     1801 tttggtaatt ttcaagaatt gcagccactg tacgtccaac tgcaagtcca gaaccgttca
     1861 aggtatgaag gagtttcacc ttgccatctg cttcatcacg gtaacggatt tgggcacgac
     1921 gggcttggaa atcttctgtg tttgaacagc ttgagatttc acggtaattg ttttgtgctg
     1981 gaatccacac ttccaagtcg taagtcttcg cagctgagaa gcccatatct ccagtagaga
     2041 gagcaacgac acggtatgga aggttgagtt tttgaagaat gttttcagcg ttggctgtca
     2101 ttttttccaa ttcttcgtaa gattcttctg gtttggcaaa tttgaccatt tcaaccttgt
     2161 ggaattggtg caaacggatc aagccacgcg tatcacgacc ggcagaacca gcctcagaac
     2221 ggaatgacgg actcatggca gtgaagtaga ttggaagatc tttgccgtct aagatttcat
     2281 cacggtagta gtttgtcaga ggaacttcag cagttggaat caagacaaag ttggtatcgc
     2341 tgagttcaaa agtatcttcc ttaaattttg gatactgacc agtaccaaac atagaatcat
     2401 ggttgactat gtaaggtgtg atgacttcag tatagccttc ttttccatgt tcatccaaca
     2461 taaagttgta gatagcacgt tccaaacgag caccgaggcc tttatagaag aggaagcgag
     2521 cgcctgttac cttaccaccg cgttcccagt caaggatacc aaggtcttca ccgagatccc
     2581 agtgagcttt aggttcgaag tcaaactcgc gtggagtacc ccaacggcga acttccacat
     2641 tgtcgtcttc gtcagcccca acaggaacgc tgtcagctgg gatatttgga agagtcgttg
     2701 taaattctgt caatttagca tcgatttctg ccaattcagc atccaaggct ttaacctcag
     2761 cagatagatt ttgcatggca gcaatcttgt catctgtatt ttccttgttg cgcttagctt
     2821 gggcaatctc agcagaaact gtgttacgtt ctgctttgag agtttcaacc ttgaccaaga
     2881 tgttacgacg tttagcatcg atttctttca tttcattcaa gacagcagca tctacaccac
     2941 gtgtagctaa tttttctgcg acagcttcaa aatctgtacg aatacgtttg atatctaaca
     3001 taagaactcc tttatgaaaa aagcaccctg acaaaacgtt ggagtggcag ggccacggtt
     3061 ccatccaact tcacaggtgt gcacttgatt gtgtatgtaa ttgtcactaa cggtagaatt
     3121 tcacctatcc ctcctatctg ctcgcagcac ccgcagactt tctgaaagaa gaagataacc
     3181 tacttatccg ttgctatgat tatactaaag tttctacttt tttgcaaata gatttttaaa
     3241 tttttggcta attgtctgaa tcagggtcgg aagtttgacg accttgtcat tgcctagttt
     3301 ttcgcgtgca attttgagaa tggcacctga gtcttttgaa gcaaagagga attttccttt
     3361 gtctgtaaag acttcgaagt ggcggctgat tttgcgtcca gtgacattgg ctccaatctg
     3421 attgatatgg ctccaaggaa tctggataaa ttgttcgaca ttgacatctg ggtaaaattc
     3481 caaagcctga tctccgacaa ggaatttccc aactttccca gcgatagaga ggtaggaagt
     3541 gcctgtcgta ctgaggagta ctgttttgtt aagtgattgg gccatgctta gtcttcctta
     3601 ctttctccaa aaaaggcatt gtagagggct ttaattgctg ctttctcttg gtctttattg
     3661 acaacaaaca taatagaaac ttcactagaa ccttgagaca tcatctggat gttgattttg
     3721 ttttcagata gagcgcgtgt cgcagtagca gtcactccga tatggctctt cattttttca
     3781 ccaacaatca taatgataga aaggtcgtgt tcgatttctg catgatctac tttagccttt
     3841 tgaaccaact gacgcaggat ttcttcttcc ttgatgggag ttagttggcg agaacggaga
     3901 atgatagaaa gatcgtcgat acctgttggc atatgttccc aaccgatgtt aagttcttcc
     3961 aggatttgca gaaccttgcg gccaaatcca acctcacggt tcatgaggta tttcgacatg
     4021 ttaatgctga caaagcctga gtcaccagca attcccacaa ctggaaattc atcattactg
     4081 tgttttagaa cgatacgagt acctggatgg tcagggttgt tggtattctt gataaccaga
     4141 ggaatttttc cacggtaggc aggaagaaga gcctcgtcat gaaggactga gaagcctgca
     4201 taggccaact cgcgcatttc acggtaggtc aactcaggaa tcgagtgtgg ttggtggata
     4261 ataccagggt gggctgcaaa gataccatca acgtccgtaa agttttcata gaggtcagct
     4321 ttgacaccag cagcaatgat agaacctgta atatcagaac ctccacgtga gaaagtacag
     4381 atttgatttt ccttagtgac accaaagaaa ccaggaatga caaggacttc attggtgttt
     4441 gtcaattctt caatcttgtc ataacttgat ggaatgatgc gagcgtgacc aggttcactt
     4501 gtgaccacaa tcccagcttc tctagggtgc atatagcgtg catcgatacc attttggtta
     4561 aagtaggcag caatcaattt ggcattgtta ttttcaccgg ctgctaggaa agtatcgtag
     4621 agaaattcat tttcttcaat aggaagagtg gccaaggcgt gaatgctttt agaaattttt
     4681 tctagcacag ctggttttag tcccaattca ctaaccatag cagcatagcg gtcgataatc
     4741 cagctttggc tcttgctaat atcgttaccc gcaacatagt cgcggtagta tttaatcagg
     4801 gcatccgtaa ccttagtatc ttcagcattg cgtttaccag gcgcagaaac gactacaaaa
     4861 cgacgctctg aatcgctttt gacgatgttt aaaacttttt ctaattgact agcagaggca
     4921 agagaactac ctccaaattt aacaactttc ataagaactc ctaaagtaag tattttatac
     4981 gattatagca gaaagaaagg cttttttcaa tgaagaaaat aagatgcttt ctagcctaaa
     5041 gcgagccttt cttatcggct gagacacttt cagtcgattt tttcttgctt tctgctccta
     5101 aaaaagatat actttgacag tgaaataatt taacactcaa tgaaaatcaa agagcaaact
     5161 aagaagctag acgaagtcag ctcaaaatac tgttttgagg ttgcagatgg aagctgacgc
     5221 ggtttaaaga gattttcgaa gagtataaac tgcttataaa ataaaaagga gccctgatgg
     5281 aacacattat ttatcagctt gaagaggact tggcaatcct taccttgaac cgtcctgagg
     5341 tcgcaaatgg ttttcatatt cccatgtgtg aggagatttt agaagctctg actttggcag
     5401 aagaaaatcc agctgtgcat tttatcttaa tcaatgccaa tggaaaggtc ttctcagttg
     5461 ggggagattt ggtagagatg aagcgggcag tggatgagga tgatattcca tcattgacaa
     5521 aaatcgcaga gttggtcaat acgatttctt ataaaatcaa gcaaatagcc aaacctgttt
     5581 taatggaagt tgacggtgct gttgcaggtg ccgcagcgaa tatggctgtt gcggcagatt
     5641 tctgtttggc gacggataag gctaagttta tccaagcctt tgttggtgtt ggtttggctc
     5701 cagatgcagg gggaattcat ctcttgagcc gcagtatcgg tgtgacgcgt gctgctcaat
     5761 tagctatgac aggagaggct ttaacggcag aaaaagccct agaatggggg ttggtttatc
     5821 gcgtttctga agctgaaaaa cttgaaaaga cgagagaaca gcttcttaaa aaattaagac
     5881 gtgcttcaag taattcctat gctgccatta agaagttggt ttgggagagc caatttaaag
     5941 actggcaggg ttacgctact ttagaactga acctacagaa atccttagct caaacagagg
     6001 atttcaaaga aggagtgcga gctcattcgg agagaagaag acctaaattt acaggaaaat
     6061 aaaaaatact tgcatcattc tttgaatttt gatataattc tttctgtcaa atgttttgat
     6121 tgtaaaagtt ttttgaagaa ggagggatat gaaaaatgga ctaccaacga atcaatgaat
     6181 atttaacatc tatatttaac aatgtccttg taattgagga agtgaacttg agaggtagtc
     6241 gttttaagga tatctccatc aaagaaatgc atacgattga tgtcatcggt aaggctccag
     6301 acgtgactcc aagtcaagtg tcaaaagagt tgatggtaac tcttggaact gttacgacaa
     6361 gtttgaacaa tttagagcgt aagggctaca ttgagcgagt tcggtcagaa caggatcgtc
     6421 gtgtggtgca tctgcatttg acaaagaagg gtcgcttgat tcataggctg cataaacgct
     6481 tccacaaggc catggtagaa aaaattattg atggcatgag cgaggaagaa attgctgtca
     6541 tgggtaaagg tttgactaat ctttaccaat ttttggagga tttgaaataa tggcttttgc
     6601 aaaaataagt caggttgctc attatgtgcc agagcaagtg gttacaaatc acgacttggc
     6661 tcagattatg gataccaatg atgagtggat ttcaagtcga acgggaatac gacaaaggca
     6721 tatttcaaga acagaatcta ccagtgattt ggctacagag gttgctaaga aactgatggc
     6781 aaaagctgga ataacaggaa aagaactgga ttttatcatc ctagctacca ttactccaga
     6841 ttcgatgatg ccctctacag ctgctcgtgt tcaagctaat attggcgcta ataaagcctt
     6901 tgcttttgac ttaaccgcgg cttgcagtgg atttgtattt gctctttcaa ctgctgaaaa
     6961 gtttatcgct tctggtcgct ttcaaaaagg cttggtgatt ggtagtgaaa ccctctctaa
     7021 ggcagtcgat tggtcggatc gatcaacagc tgtgttgttt ggagatggtg ctggtggtgt
     7081 cttgttagaa gctagcgagc aagagcattt cttagctgag agtctcaata gcgatggaag
     7141 tcgcagcgag tgtttaactt atgggcattc aggtttgcat tctccatttt cagatcaaga
     7201 aagtgcagat tcgtttttga agatggatgg acgcacagtc tttgattttg ccattcgaga
     7261 tgtagccaag tctatcaagc agactattga tgaatctcct atagaggtga cagacttgga
     7321 ttatctgcta cttcatcaag ccaatgaccg tattttggat aagatggcta gaaaaattgg
     7381 tgttgaccga gccaaacttc cagccaatat gatggaatat ggcaatacca gtgcagccag
     7441 tatcccgatt ttactttcag agtgtgtaga acaaggtctc atccctttag atggtagcca
     7501 gactgttctt ctatcaggct tcggtggagg cttgacctgg ggcacgctca ttcttacaat
     7561 ttaggtaatc atgtggtgaa cacattgtta taaaaaaact atttatttta aaggagtcct
     7621 atcatggcag tatttgaaaa agtacaagaa attatcgttg aagaacttgg aaaagacgca
     7681 tcagaagtaa cacttgaatc aacttttgat gatttggacg cagattcatt ggacttgttc
     7741 caagtaatct cagaaatcga agatgctttt gatatccaaa tcgaagcaga aaatgacttg
     7801 aaaacagttg gtgacttggt tgcttacgtt gaagagcaag caaaataagc agaatattag
     7861 tagaaggagt agggagaaac ccactccctc ttgtttaggt aatagtttga ctgtcaaatt
     7921 atggtgaaat cgaactatta cgtaagcaag gaaggttgga ggcactatga aaacgcgtat
     7981 tacagaatta ttgaagattg actatcctat tttccaagga gggatggcct gggttgctga
     8041 tggtgatttg gcaggggctg tttccaaggc tggaggatta ggaattatcg gtgggggaaa
     8101 tgccccgaaa gaagttgtca aggccaatat tgataaaatc aaatcattga ctgataaacc
     8161 ctttggggtc aacatcatgc tcttatctcc ctttgtggaa gatatcgtgg atctcgttat
     8221 tgaagaaggt gttaaagttg tcacaacagg agcaggaaat ccaagcaagt atatggaacg
     8281 tttccatgaa gctgggataa tcgttattcc tgttgttcct agtgtcgctt tagctaaacg
     8341 catggaaaaa atcggtgcag acgctgttat tgcagaagga atggaagctg gggggcatat
     8401 cggtaaatta acaaccatga ccttggtgcg acaggtagcc acagctatat ctattcctgt
     8461 tattgctgca ggaggaattg cggatggtga aggtgctgcg gctggcttta tgctaggtgc
     8521 agaggctgta caggtgggga cacggtttgt agttgcaaaa gagtcgaatg cccatccaaa
     8581 ctacaaggag aaaattttaa aagcaaggga tattgatact acgatttcag ctcagcactt
     8641 tggtcatgct gttcgtgcta ttaaaaatca gttgactaga gattttgaac tggctgaaaa
     8701 agatgccttt aagcaggaag atcctgattt agaaatcttt gaacaaatgg gagcaggtgc
     8761 cctagccaaa gcagttgttc acggtgatgt ggatggtggc tctgtcatgg caggtcaaat
     8821 cgcagggctt gtttctaaag aagaaacagc tgaagaaatc ctaaaagatt tgtattacgg
     8881 agccgctaag aaaattcaag aagaagcctc tcgctgggca ggagttgtaa gaaatgacta
     8941 aaacagcctt tttatttgct ggtcaaggtg cccagtatct agggatggga cgggatttct
     9001 atgatcagta tccgattgtc aaagaaacga ttgatcgagc gagtcaggtg ctcggttatg
     9061 atttacgtta tctcatcgat acggaagaag acaaactcaa tcagacccgc tatacgcaac
     9121 cagccattct agcgacttcg gttgctatct accgtttatt gcaagaaaag ggctatcagc
     9181 ctgatatggt tgctggtttg tctcttggag aatactctgc cttggtggca agcggcgcct
     9241 tggattttga agatgcggtt gccttggtag ctaagcgtgg agcctatatg gaagaagcgg
     9301 ctcctgctga ctctggcaag atggtagcag ttctcaatac gccagtagag gtcattgaag
     9361 aagcctgtca aaaagcttct gaacttggag tggttactcc agccaactat aacacacctg
     9421 cacaaatcgt cattgctgga gaagtggttg cagttgatcg agcggttgaa cttttgcaag
     9481 aagcaggtgc caaacgcttg attcctctta aggtgtcagg tccctttcac accgctctcc
     9541 ttgagcctgc tagccagaaa ctagctgaaa ctctagctca ggtaagtttt tcaaatttta
     9601 cttgtcccct agtcggcaat acagaagctg ctgtgatgca aaaagaggac attgctcagc
     9661 tcttgacgcg tcaggtcaag gaacccgttc gtttctatga aagtattggg gtcatgcaag
     9721 aagcaggcat aagcaacttt atcgagattg gaccggggaa agtcttgtca ggttttgtta
     9781 aaaaaattga tcaaactgct cacttagctc atgtggaaga tcaagcgagt ttagtagcac
     9841 ttttagaaaa atagactaaa ataagtagaa gttttgaaag gaaaaaaatg aaactagaac
     9901 ataaaaatat ctttattaca ggttcgagtc gtggaattgg tcttgccatc gcccacaagt
     9961 ttgctcaagc aggagccaac attgtcttaa acagtcgtgg ggcaatctca gaagaattgc
    10021 tcgctgagtt ttcaaactat ggtatcaagg tggttcccat ttcaggagat gtatcagatt
    10081 ttgcagacgc taagcgtatg attgatcaag ctattgcaga actgggttca gtagatgttt
    10141 tggtcaacaa tgcagggatt acccaagata ctcttatgct caagatgaca gaagcagatt
    10201 ttgaaaaagt gctcaaggtc aatctgactg gtgcctttaa tatgacacaa tcagtcttga
    10261 aaccgatgat gaaagccaga gaaggtgcta tcattaatat gtctagtgtt gttggtttga
    10321 tggggaatat tggtcaagct aactatgctg cttctaaggc tggcttgatt ggctttacca
    10381 agtctgtggc acgcgaggtc gctagtcgga atatacgagt caatgtgatt gctccaggaa
    10441 tgattgagtc tgatatgaca gctatcttat cagataagat taaggaagct acactagctc
    10501 agattccgat gaaagaattt gggcaggcag agcaggttgc agatttgaca gtatttttag
    10561 caggccaaga ttatctaact ggtcaagtgg ttgccattga tggtggctta agtatgtagc
    10621 gaaagctaga ggtgaaaaga atgaaactaa atcgagtagt ggtaacaggt tatggagtaa
    10681 catctccaat cggaaataca ccagaagaat tttggaatag tttagcaact gggaaaatcg
    10741 gcattggtgg cattacaaaa tttgatcata gtgactttga tgtgcataat gcggcagaaa
    10801 tccaagattt tccgttcgat aaatactttg taaaaaaaga taccaaccgt tttgataact
    10861 attctttata tgccttgtat gcagcccaag aggctgtaaa tcatgccaat cttgatgtag
    10921 aggctcttaa tagggatcgt tttggtgtta tcgttgcatc tggtattggt ggaatcaagg
    10981 aaattgaaga tcaggtactt cgccttcatg aaaaaggacc caaacgtgtc aaaccaatga
    11041 ctcttccaaa agctttacca aatatggctt ctgggaatgt agccatgcgt tttggtgcaa
    11101 acggtgtttg taaatctatc aatactgcct gctcttcatc aaatgatgcg attggggatg
    11161 ccttccgctc cattaagttt ggtttccaag atgtgatgtt ggtgggagga acagaagctt
    11221 ctatcacacc ttttgccatc gctggtttcc aagccttaac agctctctct actacagagg
    11281 atccaactcg tgcttcgatc ccatttgata aggatcgcaa tgggtttgtt atgggtgaag
    11341 gttcagggat gttggttcta gaaagtcttg aacacgctga aaaacgtgga gctactatcc
    11401 tggctgaagt ggttggttac ggaaatactt gtgatgccta ccacatgact tctccacatc
    11461 cagaaggtca gggagctatc aaggccatca aactagcctt ggaagaagct gagatttctc
    11521 cagagcaagt agcctatgtc aatgctcacg gaacgtcaac tcctgccaat gaaaaaggag
    11581 aaagtggagc tatcgtagct gttcttggta aggaagtacc tgtatcatca accaagtctt
    11641 ttacaggaca tttgctgggg gctgcgggtg cagtagaagc tatcgtcacc atcgaagcta
    11701 tgcgtcataa ctttgtacca atgacagctg ggacaagtga agtatcagat tatatcgaag
    11761 ttaatgtcgt ttatggacaa ggcttggaga aagaaattcc atacgctatt tcaaatactt
    11821 ttggttttgg aggccacaat gcagttcttg ctttcaaacg ttgggagaat agataagtat
    11881 gaatttaaac gatattaaag acttgatgac tcaatttgac cagtcaagtt tgagagaatt
    11941 ttcttataaa aatgggacgg atgagttgca gtttagcaag aatgaagcga gacctgtgcc
    12001 tgaagttgca actcaagtcg ctccagcacc cgttctagca acaccgagtc cagtagctcc
    12061 tacatctgct ccagcagaga ctgtagcaga agaagttcca gctccagcag aagcaagtgt
    12121 ggctactgag ggaaatcttg tagagagtcc acttgttgga gtggtttact tggctgctgg
    12181 accagataaa cctgccttcg ttacagttgg tgatagtgtc aaaaaaggtc aaacattggt
    12241 aattatcgaa gccatgaaag tcatgaatga aatcccagct cctaaggatg gtgtggtaac
    12301 ggaaattctc gtctctaacg aggaaatggt tgagtttggt aaaggattgg tacgtatcaa
    12361 atgatcgata ttcaaggaat caaagaagcc cttccccacc gttatcctat gcttctagtg
    12421 gaccgtgtct tggaagtgag cgagaatacc attgttgcta tcaaaaatgt gaccatcaac
    12481 gagcctttct ttaacggcca ctttcctcaa tacccagtta tgccaggtgt tctgattatg
    12541 gaagccttgg cgcaaactgc tggtgtgttg gagttatcaa aacctgaaaa taaaggaaaa
    12601 ctggtctttt acgctggtat ggacaaggtt aagttcaaga agcaagttgt accaggcgac
    12661 caattggtta tgacagcgac ttttgtaaaa cgtcgtggca ccatagctgt ggttgaagca
    12721 aaggctgaag tggatggcaa gcttgcagcc agtggtatcc ttacttttgc aattgggaac
    12781 taaggaggtt ctccatgttt cgaaaaattt taattgccaa tcgtggtgaa attgcggttc
    12841 gtattatccg tgcggcacgt gaattgggga ttgcgacggt agcggtttat tcaactgctg
    12901 ataaggaagc tcttcatacg cttttggcag atgaagcagt ttgtattggt cctggcaagg
    12961 caacagagtc ttatctcaat attaatgcag ttctatcagc tgcagtcttg actgaggcag
    13021 aagctattca ccctggtttt ggatttctca gtgaaaattc caaatttgcg accatgtgtg
    13081 aagaagtagg tatcaagttt atcggtccat ctggtcatgt tatggatatg atgggggata
    13141 agatcaatgc gcgtgctcag atgattaaag caggtgtgcc tgttatacca ggttcagatg
    13201 gagaagtgca taactctgaa gaagctttaa ttgttgctga aaaaattggc tatcctgtta
    13261 tgctcaaggc ttcagcaggt ggaggtggta aagggattcg taaggttgaa aaaccagatg
    13321 acctcgtttc tgcctttgaa actgcctcta gtgaggccaa ggccaattat ggcaatggtg
    13381 ccatgtacat agaacgggtt atctatccag ctcggcacat tgaggttcaa atcctaggtg
    13441 atgagcatgg acatgtgatt cacttgggtg aacgggattg ttctcttcaa aggaataacc
    13501 aaaaggtttt ggaagaaagt ccctcgattg caatcggaaa aacgctgcgt catgaaatag
    13561 gtgctgctgc tgttcgagcg gcagagtttg ttggctatga gaatgcagga accattgaat
    13621 ttcttcttga tgaagcaagt agcaatttct atttcatgga gatgaatact cgtgttcagg
    13681 tagaacatcc agtaacagag tttgtttcag gtgttgatat cgttaaggaa cagatttgca
    13741 ttgcggcagg tcagcctttg tctgttaagc aagaagatat tgtcctacgc ggtcatgcta
    13801 tcgagtgtcg tatcaatgca gaaaacccag cctttaactt tgctccaagt ccaggtaaga
    13861 ttactaatct ctatctgcca agtggtggag ttggcttgcg cgtggattca gcagtttatc
    13921 caggttatac cattccgcct tattatgata gtatgattgc caaaatcata gtacacggcg
    13981 aaaatcgttt tgacgccttg atgaaaatgc aacgtgccct ctatgaatta gagattgaag
    14041 gagtgcagac caatgcagat ttccagcttg acctcatttc agatcgcaat gtcattgctg
    14101 gggattatga tacttccttc ttgatggaaa ccttcttacc taaatatcaa gaaaaagaat
    14161 aaaatgactt taagagttta aaccgaaaag gggaatcaat ggctctattt agtaaaaaag
    14221 ataagtatat tcgaatcaat cccaatcgtt cggttaggga aaaacctcaa gctaagccag
    14281 aggttccaga tgaattattt tcccagtgtc caggctgtaa gcataccatc tatcagaagg
    14341 atctgggaag tgaacgtatc tgtccgcact gtagctatac ctttcgtatt tctgcccaag
    14401 aacgcttggc tttgacgatt gatatgggaa ccttcaaaga attgtttaca gggattgaaa
    14461 gcaaggatcc cttgcatttc cctggttacc aaaagaaact ggcatctatg cgtgaaaaaa
    14521 caggtctgca tgaagccgtt gtgacaggaa ctgctcttat taaaggtcag actgtggctc
    14581 ttgggattat ggattctaac tttatcatgg cttctatggg tacggttgta ggtgaaaaaa
    14641 tcactcgttt gtttgagtat gcgactgtcg aaaaattgcc agttgtccta ttcacagcct
    14701 ctggtggagc ccgtatgcag gaaggaatca tgagtctcat gcagatggct aagatctctg
    14761 cggcggttaa acgtcattca aatgctggtc tcttttacct gaccattttg acagatccaa
    14821 cgactggtgg tgtgacagct tctttcgcta tggaaggcga tatcattctg gctgaaccac
    14881 agagcttggt tggttttgct gggcgtcgtg tgattgaaaa tacggttcgt gaaagcttgc
    14941 ctgaggattt ccaaaaggca gaattcctat tagaacatgg ctttgtggat gctattgtca
    15001 aaagaagaga cttaccagat acgattgcta gcctagtcag attgcatgga gggagtccta
    15061 gatgaatatt gcaaaaatag tcagagaagc gcgtgagcag agtcgcttga caaccttgga
    15121 ctttgcgaca ggcatttttg atgaatttat ccaattacat ggtgaccgtt cttttcgtga
    15181 tgatggtgca gttgttggtg gtattggttg gcttggagac caagctgtaa cagttgttgg
    15241 tatccaaaaa ggcaagagtt tgcaagacaa cctcaaacgg aattttggcc aaccacatcc
    15301 agaaggctac cgaaaggcac tgcggttgat gaaacaggct gagaaatttg gccgtccagt
    15361 tgtgaccttt attaatacag caggtgctta tcctggtgtc ggagcagaag aacgtggtca
    15421 aggggaagct atcgctcgca atctcatgga aatgagtgac ctgaaagttc ctattatcgc
    15481 cattattatc ggtgaaggtg gttcaggcgg ggctctggct ctagctgtcg cggaccgtgt
    15541 ctggatgctg gaaaattcta tctatgccat tctcagtcca gaaggctttg cttccatttt
    15601 atggaaggac ggtactcgcg ccatggaagc agcagaactg atgaaaatca cttcgcatga
    15661 actgttagaa atggacgtgg tggataaggt gatttctgaa gtaggacttt ctagtaaaga
    15721 actgattaag agtgtcaaaa aagaactcca aacggagctg gctagacttt cacaaaaacc
    15781 gctagaagag ttgctggaag aacgctatca acgatttaga aaatactaag aatgactaaa
    15841 tgagactaga aaggtctcat tttttatgga aaaatgcaat tcaggttgaa aaattgacag
    15901 ttgaggagat gggctataaa atagacaaaa aagaaatata ctataaatgt gagcctacaa
    15961 caatctattt taagaaggta cttgcaatga caggtacaaa tacatttaca gttctttcaa
    16021 ctgaggactt ggagcaaact tcaggtggtc ttgctgtttg ggaagatgga tatagtagat
    16081 ggttatatta tagagaattt gctccctata tgaggcaagg ggcacttaat tcttatatag
    16141 atgcttggaa gtacggcttc cgagcagggt aatctttcaa aaaagtacga tgagaaacac
    16201 aaaaaaacta agacaatttg gaatattttt gcttattatt ttactatcta cctatttacc
    16261 acaaacaatt ggattgtatg tgacgatcat tttagggtta ggagctgatg tctattctct
    16321 tattttaaca atgggattag tgggaagttt ccttctatta atttggaggt taaaaaagaa
    16381 aaagatgctc tttatctttg agaaaaagag ctggaactgg tcatttgtct tctacttatt
    16441 tgcaacttat gtggtttatc agattctcgg taatttttgg gcacgctatg ctcatttgat
    16501 taatcatagg aatattcatg atgagtattt taccgtgctt ttttcaaatg gacaaccaac
    16561 ctttttatcg actatcttat cttttgtcct gcctgtaatc atcggtcctg tttttgagga
    16621 gactcttgat agagggtatt ttatgaatac cttctttccc tagtccaagt actatttgga
    16681 tgttatccta tctgctatca tctttgggat tagccatttg atactatctc acagagatcc
    16741 aattagttta ttatattata gtctgattgg tttctttttt gcccttgttt atcgttctac
    16801 cgacaatctg agattaacga ttctctgcca tagttttttt aactttctca atcatgcaaa
    16861 acctatctgg atttttgttt acaattatat ctattatcat ttttttagat agtgggagcc
    16921 agaaaaagtg tttgatagat atcaaacact ttttcttttt attgttcttc tgttacaaac
    16981 tggctgagca gtccattgat aaaacgggca gatttttgat cggagaagtc ctttgcaagc
    17041 tcgatagctt cattaacagc aaccagctga ggagtgtcaa atgaagtgat ttcaaagact
    17101 cccaagcgaa ggaggtttct ctccacgagc gttaagcgtt caatggtcca acctgctttt
    17161 aaatgctgag tgatttgctt atctagttct tcctttttag cttgaacacc agaaacgagg
    17221 tctatcaaaa aggctggaag ttgtacatcc gtatcttcac gatcatgagt ataggcgaaa
    17281 cgacaagcag tttcgacatc cgtaccgaac tcaaggctca tgagagcttg aaaagcgcat
    17341 ttacggagtt ggcgtctaga ttctaatagt ggactagtca ttgaggaagt cctcgtcaaa
    17401 tagatctttc aattctggtt ttggtgtttt atctgggacg atacctgcaa cgtgaatatt
    17461 gatagcagcg agttctacat cagccatatt acggacggca tctttgacag ctttctggat
    17521 agcaaccgct accttaggaa cttttactcc gtactcaagg tagagataga tatctgctgt
    17581 gagttcttcg tccacgtttt taagataaat gccacggccg agtgaaagtt ttgaaagggt
    17641 atcagacact gatctgtttg aaaaagagtg aaccccctct acctttgcag tagcgatagc
    17701 aatgattttt tcaagtacac gtggggcgat aacgatttcg ccaagttgtt cttcaattcc
    17761 catagaatga cctctttcta gagattaggc acgagaaacg taagttcctt ctgcagtgtt
    17821 gataacgagt ttttgtcctg cttcgatgaa gtctggaacg tttacgacaa gtccagtttc
    17881 catcgttgct ggtttaccag aacctgtaac agtagcacct ttgatagatg gttgagtttc
    17941 agcaactgtc aactcaacag tagtaggaac ggtgacaccg atcacttcag ttccgtagaa
    18001 ttggattttc acatcagagt tttcaaggat gtaaagcaat tcgttttcaa cattgactac
    18061 agggatttcg tattggtcgt aagtttctgt attcatgaag tatgctgtgt catccatttt
    18121 gtacaagtat tgagctggga cagtctcgat aatagcttgt tcaaattttt cctctggacg
    18181 gtagcttgtg tcaaatgtag aaccagtacg gacatcacgc aatttcatac gcatgatcgt
    18241 gtttccttta cctggtttgt ggtgactagc ttccaaaacg cgaatcaatt tgccgtcagc
    18301 tgtttcaaag gtcataccag cttttaattt acttgcttca atcatgtttt tacctcttta
    18361 ataaatatta ttactcttca ttttaccata aaatactctg gaaataaagc caaaatagtt
    18421 attgggagtt ggtagagaag aaaaaaccaa ccttagggct ggttttgttc tgtctagttt
    18481 tctttcaact tcgccaattc ctgtgcaagt agtttaaggg caacttgtgg gttggcttgg
    18541 cctttggttg ccttcataag gaatcctgta aaggccttgt cggcgttacg tttgcctgac
    18601 ttgaagtcgg caacagcagc ttcgttatcg gcaaagactt ggtggatgat tgggatcaag
    18661 atagctggat ctgaaatttg aaccatacca gctttttcca cgtattcacg cgcgccaccg
    18721 ccatttttag ctagatggac aaagactttc ttggcaatct tagatgaaat agtaccgtct
    18781 tcgatgatgg taatcatttc aaccaagttt tctggtgtca attcgatttg ttccagtgtt
    18841 ttaccttcag cattcaagaa ctgagcgact tccccttgga gccagttaga gacttgtttg
    18901 gcatcaccac ctagggcaac agctttttca aagaaatcag aagtgacttt attagcagtc
    18961 aactgactag catcgtagtc tgataaacca aggtcagata cataacgcgc acgacgttct
    19021 tttggaaact ctggcaactc agtccgcatt tcctcaatcc actcgtcaga aatttcaaag
    19081 aggggtaggt ctggttctgg gaagtagcgg tagtcagcag ccccttcctt gacacgcatg
    19141 aggatggttg ctttattcgc ttcatcgtaa cggcgtgttt cttggcggat ttgaccacct
    19201 gagcgaagaa tttcagcctg gcgttggact tcgtattcaa gacctttacg aacgtttgag
    19261 aaggagttga ggttcttcaa ttcagtcttg gtaccgaatt tctcttgacc ataaggacga
    19321 agggagatgt tggcatccac acgcatcgaa ccttcctcca tcttaacgtc agaaatgcca
    19381 gcgtactgga taacttcctt gagggctgtc agataagcat aggcttcttc aggagaacgc
    19441 atatctgcct cagatacaat ctcaatcaag ggaacccctt ggcggttgag gtcaacataa
    19501 gagtagccat ctgtaccatg ggtgttttta ccagcgtctt cctctaggtg ggcacgttcg
    19561 ataccgattt tcttggtcgt accgtcttct agttcgactt caatccagcc gttataaccg
    19621 attggctcat caaactgaga aatttggtag gctttgggat tatcagggta gaagtagttc
    19681 ttgcggtcaa agtgcatctt tttgtggatg tccatgttga gggcaagagc agccttgatt
    19741 ccggcatcga caaccccttt attgagaact ggcagaactc ctgggaaaga ccagtcaatc
    19801 acgttagtgt tggcattttg atcatttccg aagtgggcag aagtaggtga gaagattttt
    19861 gaattggtgt tgagctctac gtggacttca agtccgatga ctgtttcaaa gttcattagt
    19921 tgtcacctcc aaaaatcacg ggttgttgtt tgtggtagcc tgttgttgct tcaaaggcag
    19981 cagcaacttg gtaaatggtt tcctcagagt gcttgggacc aatcaattgc agaccgacag
    20041 gtagaccttg agagaatcca gcaggaatcg aaattccagg cagtcctgcc aagtttacag
    20101 gtatggtcaa taggtcggct aagtacatgg caactgggtc atggttgaga gaatccaagt
    20161 cataggcaac acttggagca gttggaccca aaatcaaatc gtaatccgcg aagacttttt
    20221 cgaaatcttg aatgataagt gtacggacct gtccagcctt cttatagtag gcatcgtagt
    20281 aacctgatga aagactgaaa gtacccagca tgatacggcg cttcacttct tcaccaaaac
    20341 cttggcttcg gctgtttaca tagatttcat caaggttggt tgcatcttct gcgcgatagc
    20401 cgtaacggat accgtcaaag cgttgcaagt ttgatgaagc ttctgatgaa gcgatgatgt
    20461 agtatacggc aactccgtat ttagagtgag gaaggctgac ttcttcgaca atagcaccca
    20521 atttttcaaa gtgtttagcg gcatttagaa tggtttcctt aacctctggg ttaattcctt
    20581 caccgaggta ttccttaggc aaagcgattt tcataccctt gatgtcttgg ccgatttttg
    20641 aagtaaagtc ggcgatgcgg acaggagcag aagtagagtc tttagcatct tcgctggcaa
    20701 tagcgttgag caagagggca ttttccttaa cagtaggagc aaaaggtcca atctggtcta
    20761 atgagctacc aaaggcaatg agaccgaaac gtgaaactgt tccgtaggtt ggtttgagac
    20821 caacgattcc gttgaaggca gcaggttggc ggatggaacc accagtatca gaaccaagtg
    20881 acaagcgaac ttgtcctgag gctacagctg cggcagaacc acttgatgac ccaccaggaa
    20941 ccttgctgtg gtcccaagcg tttttagttg ctccgtagtg tgaagtttca cctgaaccac
    21001 ccatagcaaa ttcgtccatg ttggtctttc caacgacaat catgcccttg gtttttgcat
    21061 tggcaacagc tgtcgcatca aagattggct catagttgta gagcattttt gaggcagcag
    21121 ttgtgagaat accgtctgta gagatgttat ccttaacagc aagtggaatt cctgaaagga
    21181 cattgtcagc atcaattcca gcttcatcaa tggctttagc ttgaacaaga gcttgctcct
    21241 cagcgatggt gacaaatgaa ttgagggctt cctcacgaga cttgatattt tcaagtgttg
    21301 cttgggtcaa ttctgttgca gaaatttcct tagagacaag gagattgtgc aactcttcaa
    21361 tagttttatt gttaaaagtc attaggcatc tcctccatcg tctaggatag ctggcacctt
    21421 gatatagtag ttgtcttttt caggtacgtt tttaaacaag cgatcacggt ctgttccttc
    21481 ttcggccaca tcagggcgga gtacagtctt gcggtcagcc atagtcgtag taggtgcgac
    21541 accagttgtg tcaacttcgc ccagcaattc aaccatgtca acaatcttag acaaggtggt
    21601 cgcaaaggca gcagtttctt tttcagagaa tcttaatttt gaaagattgg caacgtgtgt
    21661 tacctcttct tgcgtaattt tcatcttgca tcctttcgtg aaatgatgat ttttagtctg
    21721 ttctatttta ccatattttc ctataaataa gggaggggga agtgaaaaga aatagaaatt
    21781 gaaaaaaatg ctaaaatccg atataatgga gtgttgaaag gaatggtaaa atccaatgta
    21841 aaaatcattc tcagctattg aaacaagaaa aatagagaat caaagaaaga gaacttatga
    21901 atattcaaga agaaattaag aaacgtcgta cctttgccat tatctcccac ccggacgcgg
    21961 ggaaaacaac catcactgag cagttactct actttggggg tgagattcgt gaggctggta
    22021 cggtaaaagg gaagaaaaca gggacttttg ctaaatctga ctggatggat atcgagaagc
    22081 aacgtgggat ttctgttact tcatctgtta tgcaatttga ctacgacggc aagcgcgtga
    22141 atatcttaga cacgccaggg cacgaggact tctcagaaga tacctatcgt accttgatgg
    22201 cggtggatgc tgcggtcatg gtcgtggact ctgccaaggg gatcgaggct caaaccaaga
    22261 aattgtttga ggttgtgaaa catcgtggca ttccagtctt tacctttatg aacaagctgg
    22321 accgtgacgg tcgtgagcct ttggatctct tacaagaatt ggaagaaatc ttgggcattg
    22381 ctagctaccc tatgaactgg cctatcggga tggggaaagc ctttgagggc ttgtatgacc
    22441 tctataacca acgtttagag ctttataaag gggatgagcg ttttgccagt ctagaagatg
    22501 gggacaaact ttttggtagc aatcctttct acgagcaagt caaggatgat attgagcttt
    22561 taaatgaagc cgggaatgag ttttcagagg aagctattct ggctggagaa ttgacgcctg
    22621 tctttttcgg ttcagccctg acaaactttg gtgtgcagac cttccttgaa accttcctca
    22681 agtttgctcc agaaccacat ggtcacaaga aaacagacgg tgaaattgtg gatccttatg
    22741 acaaggattt ctcaggattt gtctttaaaa tccaagccaa catggaccct cgtcaccgtg
    22801 accgtattgc ctttgtccgt atcgtatctg gcgaatttga gcgcggcatg agtgtcaatc
    22861 tccctcgtac tggtaagggg gctaaactgt ctaatgttac tcagtttatg gcggaaagtc
    22921 gtgagaatgt gatcaatgcc gtggcaggtg atattatcgg ggtttacgat accggtactt
    22981 atcaagttgg ggataccttg acagttggta aaaacaagtt tgaatttgaa ccactgccaa
    23041 cctttactcc tgaaattttc atgaaagttt ctgctaagaa tgttatgaag caaaaatcct
    23101 tccacaaggg gattgagcaa ttggtgcaag aaggagccgt tcagctttat aagaattacc
    23161 aaacaggtga gtacatgcta ggcgctgtag gtcaactcca gtttgaagtc tttaaacacc
    23221 gcatggaagg cgagtacaat gcagaagtag ttatgaaccc aatgggtaaa aagaccgttc
    23281 gttggatcaa gcctgaggac ttggatgaac ggatgtcgtc aagtcgcaat atcttagcca
    23341 aagaccgttt tgaccaacca gtctttctct ttgaaaatga ctttgccctc cgctggtttg
    23401 cggacaagta tccagacgta gagttagagg agaagatgtg actcagtacc aacaattgga
    23461 actaaagttc caaattgttg gacgctagtc gctatttggc gaactagcta actgcctcac
    23521 taactacgtt tggcaaatag aatcgatttg ccaaactccg tgtcgtaatg taaaaattgt
    23581 aaaccgtaat gggacttcat ctaaaatctc cactggtatg gttttacgaa cgaataggcg
    23641 aaaataatgg ttacgtagaa acatatgatg ggaataaaag aaatgagata aaaagtgaaa
    23701 gaagagaaaa aagcgattgt cttaggtgca gataatgctt atatggataa agttgaaaca
    23761 acaataaaat ctctttgtgt tcatcattat aatcttaagt tttatgtttt taatgatgac
    23821 cttccgcgag aatggttcca attaatggaa aaaagactag agactttgaa tagtgagatt
    23881 gtgaatgttt agattgacag tagtattctg aaaggttatc gtctcccctt tgagggactt
    23941 tcatatgcgg cttttttagg tattttattc caaagtatgt aagcgagtct cgtgtactgt
    24001 atttggattc agatattgtt gtaagaaaat cgatagatga gctgtgggat ttggacttga
    24061 cggctatatc cttagctgca gttagagatg atttttatac acataatttt aattctggta
    24121 ttctgttaat caacaatggc atgtggagag cagaaaatgt cacgcaagac ctgatttaag
    24181 acacaaataa aaaatcttcc aattcccttg caaaaaataa gaaagtgtga taacatagta
    24241 cggtatgtgg tgctagcaca tccgctatat tagatctaat aggaggaaaa cacaatggct
    24301 aaagtatgtt actttacagg tcgtaagact gtatcaggaa acaaccgttc acacgcgatg
    24361 aaccaaacaa aacgtgccgt aaaaccaaac cttcaaaaag ttactgttct tatcgatggt
    24421 aaacctaaaa aagtttgggc ttcagctcgt gctttgaaat caggtaaagt tgaacgcgtt
    24481 taataataaa agcaaaggag accttagggt cttttttctt ttattgtggg tataacatcg
    24541 tttgaaaaat agagtaaata tccgctgata catcattctg cttttacact tgggctgaaa
    24601 tatgataaaa tagagtatca actagttgag gtaaaaagga tgactgtaaa aattaataca
    24661 aaagatggtc aaatcgaact aacagatgaa gtgattgcaa ccgtcgtagg tggagcagca
    24721 actgagattt ttggtgtggt cggtatggct agtaaaaatg ccctcaaaga taatttccaa
    24781 gccctgctag gtaaggaaaa ttattctaaa ggtgtcgtcg taaaggcagc cgaagatggc
    24841 agtattgcag ttgatgtata taccgtgtta agctacggaa caaagattag tgaagtgtca
    24901 aaaaacattc aagagcgtgt tgcctttagc ttggaaaacc agcttggaat tactgctcag
    24961 actgtaaatg tctacattca aaatatcaaa gttgtaggag aataaccgtg tcaaaaatta
    25021 ctactagctt atttcaagaa atggtgcagg ctgcatcaac tcgcttgaat aagcaagctg
    25081 aatatgtcaa ttcattaaac gtctttccag ttccagatgg agatactggg acaaatatgg
    25141 gaatgaccat tgaaaatggt gctaaagaag ttgcagacaa gccagcttct acagttggag
    25201 aagtagcgag cattcttgcc aaagggcttt tgatgggtgc gcgtgggaac tcaggagtga
    25261 ttacgtctca gcttttccgt ggattttccc aagctatcaa ggataaagac gagttaacag
    25321 gtcaagactt ggctctggcc ttccaatcag gtgtggaagt tgcctataag gcagtgatga
    25381 aacctgttga aggaacgatt ttgacagttt ctcgtggagc tgctatcggt gctaagaaaa
    25441 aagctgagca aacagatgac actgttgaag tcatgcgcgc agccttggaa ggtgctaaaa
    25501 cagctctagc taaaacacca gacatgcttc cagtattgaa agaagttggc gttgtggact
    25561 caggtggtca aggattggtc ttcatctacg aaggtttcct ttcagccctt actggcgaat
    25621 atattgcatc tgaggacttt gtagcgactc ctgccaacat gagtgagatg atcaatgtag
    25681 agcatcataa gtctgtagct ggtcacgtag cgactgagga catcacgttt ggttactgta
    25741 ctgaaatcat ggtagctctt aagcaaggtc caacctatgc taaagatttt gactacgatg
    25801 aattccgtaa ctacttggat gagcttggag attctctcct cgttgttaac gatgatgaaa
    25861 ttgtcaaagt ccatgttcat acagaagatc caggacttgt tatgcaagaa ggtctcaaat
    25921 atggtagctt ggtcaaggta aaagttgaca atatgcgtaa ccaacacgaa gcacaggttg
    25981 agaaagaagc tactcaagtt atcaagtcgg ctgaagaaaa agagtatgct ttgattgctg
    26041 tggtggctgg taaaggtcta gcagatatct tctgttctca aggcgtggat tatgttatcg
    26101 aaggcggtca aaccatgaac ccttcaacag aagactttat caaggctgtt gaacaggtta
    26161 atgcccgtaa catcatcttc ttgccaaaca acaagaacat cttcatggca gctcaatctg
    26221 cggcagaagt tttggagcaa ccagcggtag tggtagaggc tcgcactctt cctcaaggta
    26281 tgacaagtct tcttgccttt gatccaagca agtccattga agaaaaccaa gagcgtatga
    26341 cagctgctct tagcgatgtc gttagcggaa gcgtcacaac agccgtgcgt gatacaacga
    26401 tcgatggctt agaaatccat gaaaacgata atttaggtat ggtggatgga aaaattcttg
    26461 tgtcaaaccc tgatatgcac caaacattga ctgaaacctt gaaacatatg ttggatgaag
    26521 acagtgaaat cgtaaccttc tatgtcggtg aagacggaag cgaagaactt gccaatgaaa
    26581 tcgctcaaga aatcgtagaa gaattcgaag acgttgaagt cgagattcac caaggtcaac
    26641 aacctgttta cccataccta tttagtgtgg aataagatta atgaaggact gagaaatcag
    26701 ttcttttttc ttt
//
LOCUS       DAVLSL010000015        24701 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLSL010000015 DAVLSL010000000
VERSION     DAVLSL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24701
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.015"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(403..1251)
                     /locus_tag="VXN30_000496"
     CDS             complement(403..1251)
                     /locus_tag="VXN30_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1357167.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1403..1681
                     /locus_tag="VXN30_000497"
     CDS             1403..1681
                     /locus_tag="VXN30_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357168.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1683..3746
                     /locus_tag="VXN30_000498"
     CDS             1683..3746
                     /locus_tag="VXN30_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1357169.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3824..5080)
                     /locus_tag="VXN30_000499"
     CDS             complement(3824..5080)
                     /locus_tag="VXN30_000499"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1357170.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVIVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5222..7687
                     /locus_tag="VXN30_000500"
     CDS             5222..7687
                     /locus_tag="VXN30_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1357171.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7752..8663
                     /locus_tag="VXN30_000501"
     CDS             7752..8663
                     /locus_tag="VXN30_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1357172.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            8893..9591
                     /gene="dapD"
                     /locus_tag="VXN30_000502"
     CDS             8893..9591
                     /gene="dapD"
                     /locus_tag="VXN30_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1357173.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9659..10789
                     /locus_tag="VXN30_000503"
     CDS             9659..10789
                     /locus_tag="VXN30_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1357174.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10801..11340
                     /locus_tag="VXN30_000504"
     CDS             10801..11340
                     /locus_tag="VXN30_000504"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1357175.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRTLSTVYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11324..12001
                     /locus_tag="VXN30_000505"
     CDS             11324..12001
                     /locus_tag="VXN30_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1357176.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQIWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(12068..12967)
                     /gene="galU"
                     /locus_tag="VXN30_000506"
     CDS             complement(12068..12967)
                     /gene="galU"
                     /locus_tag="VXN30_000506"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1357177.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(12989..14005)
                     /locus_tag="VXN30_000507"
     CDS             complement(12989..14005)
                     /locus_tag="VXN30_000507"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1357178.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14217..14597
                     /locus_tag="VXN30_000508"
     CDS             14217..14597
                     /locus_tag="VXN30_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1357179.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(14771..15196)
                     /locus_tag="VXN30_000509"
     CDS             complement(14771..15196)
                     /locus_tag="VXN30_000509"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1357180.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(15264..15914)
                     /gene="phoU"
                     /locus_tag="VXN30_000510"
     CDS             complement(15264..15914)
                     /gene="phoU"
                     /locus_tag="VXN30_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1357181.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(15929..16681)
                     /gene="pstB"
                     /locus_tag="VXN30_000511"
     CDS             complement(15929..16681)
                     /gene="pstB"
                     /locus_tag="VXN30_000511"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1357182.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16683..17498)
                     /gene="pstA"
                     /locus_tag="VXN30_000512"
     CDS             complement(16683..17498)
                     /gene="pstA"
                     /locus_tag="VXN30_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1357183.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17491..18354)
                     /gene="pstC"
                     /locus_tag="VXN30_000513"
     CDS             complement(17491..18354)
                     /gene="pstC"
                     /locus_tag="VXN30_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1357184.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18472..19347)
                     /locus_tag="VXN30_000514"
     CDS             complement(18472..19347)
                     /locus_tag="VXN30_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1357185.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19514..20845)
                     /locus_tag="VXN30_000515"
     CDS             complement(19514..20845)
                     /locus_tag="VXN30_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1357186.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20846..21553)
                     /locus_tag="VXN30_000516"
     CDS             complement(20846..21553)
                     /locus_tag="VXN30_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1357187.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21637..21939)
                     /locus_tag="VXN30_000517"
     CDS             complement(21637..21939)
                     /locus_tag="VXN30_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1357188.1"
                     /translation="MTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(21926..22147)
                     /locus_tag="VXN30_000518"
     CDS             complement(21926..22147)
                     /locus_tag="VXN30_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1357189.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            22109..22668
                     /locus_tag="VXN30_000519"
                     /pseudo
     CDS             22109..22668
                     /locus_tag="VXN30_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(22983..24674)
                     /locus_tag="VXN30_000520"
     CDS             complement(22983..24674)
                     /locus_tag="VXN30_000520"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1357190.1"
                     /translation="MNTKELIASKLSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTIAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
BASE COUNT         7136 a         4736 c         5363 g         7466 t
ORIGIN      
        1 gcgggaaaaa tcgaaatact tgatttatac ggcctatatc atatttgggg taaatgtagt
       61 atatggtatt caatggttat taagagaact gatttcaact atttcccctt aatgtgtatg
      121 gaaactataa caaaacgcat aagattaagg tttgtgtacc tgttcttatg cgtttttata
      181 atattgaaga ttttcggtaa gttttctact tttgcgaagt ctagtttata ctcaatgaaa
      241 atcaaagagt aaactagccg taggttgctc aaagtacagc tttgaggttg cagataaaac
      301 tgacgaagtc agctcaaaac atggttttga ggttgtagat gaaactgacg aagtcagtaa
      361 ccatacgtac ggcaaggcga cgttgacgtg gtttgaagag tattagaatg ctttcccaac
      421 caaaatctct gcttcgatgg taatctctgt cagttggctc cagtcaatct tgctctggtc
      481 aacgtgaaag aggagaggag tcatactgag tagggcttgg agttgttctg ctgtgatagt
      541 cttagtcaga gaggcagttt gactagatag gatggtaaag tgttcctgga aatgctcctt
      601 gatatcttgg ttagaatact ccttgtttgt cagctggtcc tgtactcttt gacggatttc
      661 tttgaggtga ttttcagttg ggataacctt tatcaagata ccgtctttgg ataaaacgcg
      721 acgaaattct ccatagttgg caggtgagaa gatatcaagc agaatatcca tgttagcgtc
      781 ttttatagga aggcgtgcca agtcgccaac gaaccaattg actgcccagt tgggttcact
      841 tttagcagcg atttggactg aatctttgga gatatcaaag gcatagaaag ttttttcaga
      901 gtgactttct tgtagtttgc gagaatagaa tccttcacca caaccgatat ccaaaattgt
      961 tgtggtagtt tttgagcttg caagcaagtc agatacagca tctaagatag cttggtaaaa
     1021 gccggcttct aggatttgtt gacggttttg aaaattttcc ttgtcatagt tagcagattg
     1081 cttgatttga ggtgctagat tgacatagcc aaatttcgcc aagtcaaaag aatgacggtt
     1141 gcagcacttg aaattagtct ctaacagagt cagattttct tgacagatag gacaggcaaa
     1201 ggcagtcgca gaagcaaaac gctgaagttt gggcttgaga tttgtattca tagtttaagt
     1261 gtatcaaaag aggcaaatga aagcaacttt aggaataagt agatgggaga ttcagtagaa
     1321 ataaaactaa ttctccaatt tatagaatga aattgctttt atatctaaat tgctatataa
     1381 taatgataag gaggaaataa gtatgttaaa agaagtatta accgttgcaa aagttgcgaa
     1441 aaaatcttca ttatttttgg gtggtgtcgc atttggtacc cttggtttga aaatcttagc
     1501 aagtaaggaa gctaaaaaag gttattctaa agctttggct aaggcttaca agttgaaaga
     1561 cgagctagat gcatctgttt ctgttgtgaa gcaacatgga gacgatgtct tgcaagatgc
     1621 caaatatttg tacgagcaag agaaaaaaga agagcaatta gatagcctta taggtgaata
     1681 atatgtcttt taaagtgcta catagaggat accaacatat ccgactatca tcttcttttt
     1741 cacttacctt ggatattcaa gactatcttc gttccttggc gagagatgaa aaggggattg
     1801 agtctatcca gttttacatg gatcaacagc actttactct acgcataaaa gaaggctttt
     1861 ctgtattaga taatgcagaa gcctttttaa aaagaattga taaagggaaa gtttctgagt
     1921 tgatgactct tcccattcgt agagaagaga gtgcttattc tattgtttca ggtgcagcgg
     1981 ttaagcgtgt actttttcgt agttttgtgc cgtatcctat tcgctatata tggacttgtt
     2041 atcaggcttt gggttatatt agagaagcct atcaaacact agcgcgtaag gaactaacta
     2101 tggaagtctt ggactgttcg gctattttat tgtctttgtt tatgaaccaa tccaagacag
     2161 ctagcaatat catgtttatg cttgatttgg ggaatcattt agatcagtgg tccttgaaaa
     2221 aaactgcaac agatttagaa cagagtcttc ttgcaaaaga gagcgatgta ttcctagtac
     2281 agggcgatac ggttgttagt ataaagagtt ccgatgttca aataggagat gtcttgatct
     2341 tatctcaagg aaatgaaatt ctgtttgatg ggcaagtagt ttcaggttta ggtatggtca
     2401 acgaaagttc cttgacagga gagagttttc cagttgaaaa aagagagtct gatttggttt
     2461 gtgcaaatac agtattagaa actggagagt tacgcattcg tgtaacagat aatcagatga
     2521 acagccgtat tttacagctg attgagttga tgaagaaatc tgaagaaaac aagaaaacga
     2581 aacaacgcta tttcatcaag atggcggata aagtcgtcaa atataatttc ttgggggctg
     2641 ggctgactta cctattgaca ggttcttttt ctaaggctat ttctttccta ttggtcgatt
     2701 tctcctgcgc tttgaaaatc tctactcctg tagcttattt gacagttatc aaggaggggt
     2761 tgaaccgtga aatggtgatt aaggatggag atgttctgga gaaatatctg gaagttgata
     2821 ctttcttgtt tgataagaca ggaacaatca caactagtta tcctatagtt gaaaaggtgt
     2881 taccttttgg agactatagt gaggaagata ttctcagaat cagtgcctgt cttgaggaac
     2941 acatttatca tcctattgct aatgccatcg tcaagcaagc tgagatagag ggaattgaac
     3001 atgaggaaat gcatgggaaa ctccaatata tcgcaagcaa ggggatcaaa tctcatatag
     3061 atggccaacc agttcttatt gggaattatg tcttgatgca ggatgagcag attcatatca
     3121 gttcagaaca aaatgcttta attgaagagt acaagagtca ctacaatctc ttattcttgg
     3181 cttatcaaaa tgaattgatt ggaatgttct gcattcatac tcctttgaga aaagaagcaa
     3241 aaacagcctt ggataaactt aaggcacagg ggaaaaaatt gattctggca acaggagaca
     3301 ccttgattag gacagaggaa ttagtcaaag atttgccctt tgatcaggtc tatacagact
     3361 tgaaacctga tgggaaattt gagttagtag agaaactgca gaaagcaggt cacactattt
     3421 tgatggttgg agatggattg aatgactcag cggctctaac tctatcagat atcggtgtgg
     3481 tgatgaatga gagtgcagat atttctaagc agatgagtga tatcttattg ttagataatc
     3541 gtttggattt cttccaagag ttggattcgt tatcatcatc tttgcaaaca ctcatcaaga
     3601 aaaatattca agataccgtt gtcgtaaata gtagtttgat tggcttcggc ttatttaatt
     3661 ggctcagtcc ttcaaatctc tctatcttac ataatctaac aaccttgcgc attgtcctgc
     3721 gtagcctgtc tattaaaaat agatagatga gagttattaa cagcaaaaag gagttacctt
     3781 tctcgaggtt aactcctttg tttatagata aatgttgaat agtttagtaa gtcaatacaa
     3841 agtatttttt cttcccacga cggataacag tcagttcatt ttctaactta tcagcgtcac
     3901 tcaagacata gtcaagctct tggatgcggt cgccgtttac gtagatggct ccgttttgga
     3961 cgtcttcacg ggcttggcgt tttgagttaa ctataccaga tgagacgagc agttccacga
     4021 tattgttgtt ttcgtctgcc tgtacttggt agtttggcac accacgaagt ccttgtttga
     4081 gctctttgac agaaaggttt ttgatgtttc ctgcaaagag ttgctcagtg atgttaagtg
     4141 cttctttgta ggcttcttct ccgtgaacaa gtgtaacaac ttcacgagcc aagacttttt
     4201 gagccaagcg ttcgtgtggc gctgcttcaa attgtttacg aatatcttca atctcatcaa
     4261 gtgacaagaa agtaaagatt ttcaagaagc gaacagcgtc agcgtccatc acgttcatcc
     4321 agaattggta catttcgtat ggagaagtct tttcgggatt gagccagacg gcatttcctt
     4381 ctgatttacc aaatttctta ccagttgcat ctgtgattag tggaacagtg ataacgtgac
     4441 cagtcttgtc cgccttacga cgaagcaatt cggtaccagc tgtcatattt ccccactggt
     4501 cagaaccacc gatttgaaga gtgacattat ggtcttggtt aaggacgaag aagtcatacc
     4561 cttgcatgat ttggtaagcg aactcagtgt aagaaattcc tgtttcgatc cgttttttaa
     4621 cagattcctt actcatcatg tagttgaccg tgaagtattt tccaatatca cggaggaagt
     4681 caatgaagct gatgctgcca aaccagtcgt agttgttgac tatgacagcc ttgttttcgc
     4741 cattttcaaa gtcaagaaaa cgagaaagtt gtccttggat agacttgacc cagccatcta
     4801 ctgtgtcttt tgtttggaga ctacgttcag catctttgaa ggacggatct ccgatgagac
     4861 ctgtagcacc gccaacgagc gcataaggtt tgtgacctgc tagttgcaag cgacgacttg
     4921 tcaagattgc gacaaggtgg cctaggtgaa ggctgtcagc agttggatcg tagccagtat
     4981 aataagaaac ttgaccttct tctagggctt tacgcaaagc ttcttcatca gtcgtttgaa
     5041 atatcaaacc acgctctttt agctcatcaa aaatgtgcat gtgttttttc tcctttttaa
     5101 aattattgtt tctacctatt gtatcacaaa cttgggcaat agcctagcag aatagggaag
     5161 aaagtggcta ttttattgaa aatttccctt ttgtggtata atagataaag tgaggaaatc
     5221 catgcaaaat caattaaatg aattaaaacg aaaaatgctg gaatttttcc agcaaaaaca
     5281 aaaaaataaa aaatcagcta gacctggcaa gaaaggttca agtaccaaaa aatctaaaac
     5341 cttagataag tcagccattt tcccagctat tttactgagt ataaaagcct tatttaactt
     5401 actctttgta ctcggttttc taggaggaat gttgggagct gggattgctt tggggtacgg
     5461 agtggcctta tttgacaagg ttcgggtgcc tcagacagaa gaattggtga atcaggtcaa
     5521 ggacatctct tctatttcag agattaccta ttcggacggg acggtgattg cttccataga
     5581 gagtgatttg ttgcgcactt ctatctcatc tgagcaaatt tcggaaaatc tgaagaaggc
     5641 tatcattgcg acagaagatg aacactttaa agaacataag ggtgtagtac ccaaggcggt
     5701 gattcgtgcg accttgggga aatttgtagg tttgggttcc tctagtgggg gttcaacctt
     5761 gacccagcaa ctaattaaac agcaggtggt tggggatgcg ccgaccttgg ctcgtaaggc
     5821 ggcagagatt gtggatgctc ttgccttgga acgcgccatg aataaagatg agattttaac
     5881 gacctatctc aatgtggctc cctttggccg aaataataag ggacagaata ttgcaggggc
     5941 tcggcaagca gctgagggaa ttttcggtgt agatgccagt cagttgactg ttcctcaagc
     6001 agcattttta gcaggacttc cacagagtcc cattacttac tctccttatg aaaatactgg
     6061 ggagttgaag agtgatgaag acctagaaat tggcttaaga cgggctaagg cagttcttta
     6121 cagtatgtat cgtacaggtg cattaagcaa agacgagtat tctcagtaca aggattatga
     6181 ccttaaacag gactttttac catcgggcac ggttacagga atttcacgag actatttata
     6241 ctttacaact ttggcagaag ctcaagaacg tatgtatgac tatctagctc agagagacaa
     6301 tgtctccgct aaggagttga aaaatgaggc aactcagaag ttttatcgag atttggcagc
     6361 caaggaaatt gaaaatggtg gttataagat tactactacc atagatcaga aaattcattc
     6421 tgccatgcaa agtgcggttg ctgattatgg ctatctttta gacgatggaa caggtcgtgt
     6481 agaagtaggg aatgtcttga tggacaacca aacaggtgct attctaggct ttgtaggtgg
     6541 tcgtaattat caagaaaatc aaaataatca tgcctttgat accaaacgtt cgccagcttc
     6601 tactaccaag cccttgctgg cctacggtat tgctattgac cagggcttga tgggaagtga
     6661 aacgattcta tctaactatc caacaaactt tgctaatggc aatccgatta tgtatgctaa
     6721 tagcaaggga acaggaatga tgaccttggg agaagctctg aactactcat ggaatatccc
     6781 tgcttactgg acctatcgta tgctccgtga aaatggtgtt gatgtcaagg gttatatgga
     6841 aaagatgggt tacgagattc ctgagtacgg tattgagagc ttgccaatgg gtggtggtat
     6901 tgaagtcaca gttgcccagc ataccaatgg ctatcagacc ttagctaata atggagttta
     6961 tcatcagaag catgtgattt caaagattga agcagcagat ggtagagtgg tgtatgagta
     7021 tcaggataaa ccggttcaag tctattcaaa agctactgcg acgattatgc agggattgct
     7081 acgagaagtt ctatcctctc gtgtgacaac aaccttcaag tctaacctga cttctttaaa
     7141 tcctactctg gctaatgcag attggattgg gaagactggt acaaccaacc aagacgaaaa
     7201 tatgtggctc atgctttcga cacctagatt aaccctaggt ggctggattg ggcatgatga
     7261 taatcattca ttgtcacgta gagcaggtta ttctaataac tctaattaca tggctcatct
     7321 ggtaaatgcg attcagcaag cttccccaag catttggggg aacgagcgct ttgctttaga
     7381 tcctagtgta gtgaaatcgg aagtcttgaa atcaacaggt caaaaaccag ggaaggtttc
     7441 tgttgaagga aaagaggtag aggtcacagg ttcgactgtt accagctatt gggctaataa
     7501 gtcaggagcg ccagcgacaa gttatcgctt tgctattggc ggaagtgatg cggattatca
     7561 gaatgcttgg tctagtattg tggggagtct accaactcca tccagctcca gcagttcaag
     7621 tagtagttct agcgatagca gtaactcaag tactacacga ccttcttctt caagggcgag
     7681 acgataattc tctaaatgaa gtggccaatc aatggattgc cacttttttc ttttcgtgtt
     7741 acaataaggg tatgaatcag tatcagaaaa agattgttaa cggaaaaatt tattcgctcc
     7801 tatccggctt aatatgggga atctgtggaa ttttaggaga gtacttcttt actcattatc
     7861 aggtgtcttc gggctggatt acctctatgc gtttgacact ggcagggagt cttgtactca
     7921 tttggtctgc aatacaatta aaatcgcaag tgctagatat ttggcgagac aagaaaaatt
     7981 acctgccctt tttagcctat gctattttgg ggattttttc agttcagtat tttttctatc
     8041 tctgtgtaga atactcaaat gctacgacag caactatttt acagtttatt agccctgtct
     8101 ttatcctctt ttacaatcgc ttggtttatc aaaaacgagc gtcaaaaagc gctgttttct
     8161 atgttttggt tgccatgctg ggtgtttgct tgatggcgac aaagggagat ctctctcagt
     8221 tatccatgac gccgctagct cttataacag gtttgctgag tgccatgggt gttatgttta
     8281 atgttatctt gccccaacct tttgctaagc gttatggttt tgttcctacg gttgggtggg
     8341 ggatgatttt ggcaggtttg tttagcaatg tcctctcgcc ggtttatcag ctttccttta
     8401 ctcttgatat ttggagtatc ttgatttgcc tcattatcgc tttctttgga acggcttttg
     8461 cttttttcat ttccatgaag gctgtgtcct tggtttctcc tttggtggtt gccgttatca
     8521 gtgccagtga acctctctct tctgctctct tgagtgtttt gttcttagga ttagtagtgg
     8581 attggtccct ccttctagct atagccttga ttattttacc catgatattt ttgtctatag
     8641 aagaagcgaa agaaagtaga taaaaagtct tttcttaatt ctaaaagtgt gctatactag
     8701 aatagtcaat aaaacacggg gagatagttg tgaagagtgt ttttaggttg tatcggtagg
     8761 ggcttgcttt taccgacagc acgttttatc tcacaaccct aaaaaatcat acctaaaaac
     8821 agacaaaaag gtttcaaatt tgaggccttt tttggtagaa taggtatcat tataacgaac
     8881 caggaggcac ctatgactgc tacaaaaatg aacgctcaag aaattatcca atttatcgcc
     8941 aatgctgaaa agaaaaccag tgtcaaagta acctttgagg ggcaactcgc aactgctgtg
     9001 cctagctctg ttgtcaaact aggaaatgtc ctattcggag actggaagga cgtggctccg
     9061 cttcttgaag gtttggtaga aaatcaagat tatgttgtcg agcaagatgc tcgtaattct
     9121 gcagttcctt tgctagataa gcgtgctatc aacgctcgta tcgagccagg tgctattatc
     9181 cgtgaccagg tggaaattgg tgacaatgct gttatcatga tgggagctgt tatcaatatc
     9241 ggtgctgaaa tcggtgctgg aaccatgatt gacatgggtg ccatccttgg tggccgtgcc
     9301 atcgttggaa aaaatagcca cgttggtgca ggtgcagttt tggcaggtgt gattgagcca
     9361 gctagtgctg aaccagtccg tgtcggagac aatgttctta tcggtgctaa tgcagtggtt
     9421 atcgaaggag tccaaatcgg tagtggttca gttgtcgctg caggagctat cgttacccaa
     9481 gatgtcccag aaaacgtggt agtagcaggt gttccagctc gtatcatcaa agaaattgat
     9541 gcccaaaccc aacaaaaaac agcgctagag gatgcgcttc gtaccttgta attgtaaaag
     9601 taaaaaagag gcggaaccct ttttccagcc tctttctgct atatcggagg acagatagat
     9661 gttagatttg attcagacta gacgagcttt acaccagatt ccagagattg gcttggagga
     9721 gttcaagact caggcttatt tgctggatgt gattgagaaa ttgactacgg gcaaggattt
     9781 tgttcaaatt cgtacttggc ggacagggat tttggtctac ctgcagggaa gtcagccgga
     9841 gcgaaccatt ggttggcgga cagatattga tggtctgcct atcgtcgaac aaacaggtct
     9901 accttttgct tctcaacacc aaggtcgcat gcatgcctgt ggtcatgatt ttcatatgac
     9961 cattgcctta ggctgtcttg agcgcgccct tgaggagcaa cccaagaata atctgctctt
    10021 tctatttcag cctgctgaag aaaatgaagc tggtggtatg ctcatgtatg aggacggtgc
    10081 ttttggagat tggttgccag atcaattcta tggtctccat gttcgtccag atttgaaagt
    10141 cggccagatt gcgactaata ctcatacact ctttgcagga acttgcgagg tgaagatccg
    10201 tttcaaaggc aaaggtggcc acgcagcttt tcctcatgaa gccaatgacg ccttggtggc
    10261 ggctagttac tttgtgaccc aagtgcagtc agttgttagc cgcaatgtca acccaatcga
    10321 gggagcagtg gtgaccttcg gcgtttttca agctggaacc accaacaatg tcatcacaga
    10381 cacagctttt ttacatggaa ctattcgtgc cttgacacag gacatgagtc tcttggtgca
    10441 aaagagggta aaaacagttg cagaaggggt tgcagctgcc tttgatatgg aagtcgaagt
    10501 ggaactcaaa caaggtggat acctacctgt tgagaacaat ccagccttgg cgcgtgaact
    10561 gatggacttc tttgacgaaa aagacggaat cgagttgatt gatatcgaac ctgctatgac
    10621 aggcgaggac tttggttatc tcctttcaaa ggttgatggc gtgatgtttt ggctaggtat
    10681 cgatagtccc tacgctcttc atcaccctca aatgagtcct aaggaagaag tcttagctat
    10741 tggggtggct gcggtgtcta gtttcctgaa aaagaaggca gcagaataga gggcttgtct
    10801 atgaaatcgg aattacgcaa gcaagtcttg catgaaatga aggctttatc tcaagaacaa
    10861 aaacaggcta tagaccaagc tttaaccgag cgaattttac aacacccctt ttatcaagaa
    10921 gccaaggtca tcgcaaccta cctctctttt tctcatgagt ttcaaacgcg ggaactgatt
    10981 gagcaggcgc tgaaggacgg caagaaggtt ttaataccca aaacttatcc caaggggcgc
    11041 atggagtttg tggtctatga tccgcaacag ttggtaaaaa cttcctttgc cttactggag
    11101 ccacagggag atttggaagt ggtggatgta tctcagattg atttgattca tgttcctggc
    11161 ctggctttta cgacggatgg atatcggatt ggatatggtg gaggttatta tgaccgctat
    11221 ctggaacatt tttctggtcg tactttgagt acggtttatc cttgtcaaat tcaggacttt
    11281 atacctgaaa accatgatat tcctgttcag gaggtattaa ttgatgaagg aaatctttga
    11341 tagacgttac cctgtgacga gtttcttcct cttagtgacg gccttggtat ttttactaat
    11401 gttggtcact gcaggcggaa actttgacag ggcagataca ttatttcgat ttggagccat
    11461 gtatgggcca gctattcgcc tctttcccga gcagatttgg cgtctcttgt ctgccatttt
    11521 tgttcatatt gggtgggaac atttcattgt taatatgctt tcactttatt atcttggaag
    11581 gcaggtagag gagattttcg gttctaagca gtttttcttt ctctatcttt tatcaggaat
    11641 gatgggcaat ctctttgttt ttgtatttag tcctaaatcc ttagcagcag gcgcctctac
    11701 ctctctttat gggctatttg ccgcgattat cattcttcgc tatgcaacgc gtaatcctta
    11761 tattcagcag ttgggccaat cctatctgac actttttgtg gttaacatta ttggaagtgt
    11821 tctgattcca ggaatcagcc tagcaggcca tatcggtggt gcagttggtg gcgcatttct
    11881 agcagttatc tttccagtta gaggagaaaa acggatgtat aacaccagcc agagattagg
    11941 agcggtagtc ttgttcgtag gactcgccat tttgcttttc tacaagggaa tgggaatgtg
    12001 acgaagttga aagaaaattt gtgtaatgaa aaaagataag gcattttttg aaccttatct
    12061 ttttatttta ttccttctca gtcaattctt ttccaagttg gatgaggtaa ttcttcaaat
    12121 catctttgac ttgtgggtgt ttgagggcgt agtcgatgga tgttttcatg aagccaaact
    12181 tgtctccgac atcgtaacga gcccctttga actcacgagc aaatacacgt tgtgttttat
    12241 tgagggtgtc gattgcatct gtcagctgaa tttcatttcc tgcacctgga gcttgctttt
    12301 cgagaatctc aaaaatttca ggcgtgagga ggtagcgtcc gataatagca aggtcgctag
    12361 gagcgtcctc tggagctggt ttttcaacaa aggtttcaac actgtaaaga ccatcttttc
    12421 cttcgccttg cggagcaata accccgtaag cagatacttc gtcatgaggg actggcatga
    12481 cagcgatagt agacgcgtgg gtacgctcgt agtcatccat gagttgtttg gtaagtggaa
    12541 cagccttttc gtctgtgata tccatcaagt catcaccaag cataacgaca aaaggttcat
    12601 ttccgacgaa agccttggct tgcaaaacag catctccgag accgcgtgga tgagtttggc
    12661 ggataaaatg cagacgcatg ccagttgttt catcaactag cttcaaaaga tctgttttcc
    12721 ctttttcttt gaggttatat tccaattcga agtttgaatc gaagtggtcc tcaatagaac
    12781 gttttgattt accagtgaca actagaatat cttcaatacc tgatttgaga gcctcttcca
    12841 cgataaactg gatagttggt ttgtctacga ttggcaacat ttctttggca agggccttgg
    12901 ttgctggtaa aaatcgagtt cctagtccag cagcagggat aactgccttt ctaacttttg
    12961 atgtcataaa aatcctttct atagagggtt aagaccactc attttctgct ttaaattcat
    13021 tgttcatgat gtcataaatg gcatctttga tattggttcc gtgataaata acttggtaaa
    13081 tagcctgtgt aatgggcata tagactccaa gttcttgggc tagttcatag gctgctcgag
    13141 tcgttgaaat tccttcgatt accatgccca tattagcttc tatatcagct agggattctc
    13201 ctcgtccgag agcatctcca gctctccagt tacgagagtg gatggaagtt cccgttacga
    13261 tcaaatctcc cacaccagat aagccgctat aggtcaatgg actggccccg agtgctaccc
    13321 ctaggcgggt gatttctgct aaacctcgag cgatgatggc tgccttagca ttatcaccaa
    13381 atccaagacc atgtaaagct ccagcaccga cagcaataat atttttaaga gcaccagcag
    13441 tttcaacccc gataacatcc gtattggtat aaagtcggaa gtagtgatta ctaaatagct
    13501 tctgaacgta ttgagctgtt tgtaaatctt tagaagcagc agttattaaa gttaggtcac
    13561 gcacaatggt ctcttctgca tgactaggcc ctgaaacaac gacgatatca ctacggagat
    13621 gttcaggaat ttcttcttca agaatggttg ataatcgttt atggctatca ggttctaatc
    13681 cctttgatgc gtgcatgatg ataaccttat ggtccaaggt ttgtgcaact tgctgggcaa
    13741 caagtcgtgt cacttttgtt gggacaacaa acaaaatcgc atccacatct ttcaatgttt
    13801 ctgctaagtc ggtgtaggca atgatatttt cgtctagaac gacatcttta aagtagtgct
    13861 tattagtatg gtgtgtatta atttcattga tttgctcggg aagatttccc caaatacgta
    13921 cctcgtgtcc attgtcattt aagacttgtg aaagggcggt tccccaagaa ccaggcccca
    13981 agacggcgac ggtttgtttt tccatgattt cctccttgat agagttcttt ctcttattct
    14041 atcatattct aggggatttt gcacttgcat tactggggta tggtggaaaa atacacaaaa
    14101 accgagacga agggataaac tcttagtctt tagttaaagt gagaagaaat ctgatagata
    14161 gtttgattca gtatatgctt ataatagagg taaacaactc aggaggttct tgtaggttgc
    14221 gagagtttgg cgaaaaaatt aaaagattac gtttggctaa aaaaatcagt cgttcagaat
    14281 tttgtggtga tgagtctgaa ttaagtatcc gtcaattaat tagaattgaa aatggagaat
    14341 ccagaccaac actaacaaag ttaaaatata tcgctgaacg tttgggggtt gaggattaca
    14401 agttgatgcc aagttatata gagttggata aggaatacct agaattgaag tatttcttga
    14461 tgaggactcc tacatacgaa gatgaaacta tcgcccaaaa gaaagagagt gttttgataa
    14521 gatttttgaa gagtattatg ataggctacc tgaggaagaa agatttatca tcccaaatta
    14581 ttcatatctg gcactaacga actacacagt tcaaaaatta ccagaaaagc tagttgaaat
    14641 actgtccttc tggtgatttt gcttaatttc tgatgttaga gacataattg aggagatttg
    14701 tcttgatttt ttcctaaaaa agggcagact cctcccttga tttgatgctc gatttttaaa
    14761 ataaggtgac ttattctgtt aggcatgttt tcccctgccc tctagctcta tatggaacag
    14821 ggagtatcag atttatctgg cagattgtat caaataaggc ttgaaactgt tttgaatagg
    14881 catatagact tgagaatttg agaatatgtc gtctagcagt agagacatat tttccggcaa
    14941 tatgaaggaa gagacgtcga aaacgcttga tagtcaaggc ctttacttca tcaccagcta
    15001 gctgctttaa aaagaggtag agattataag ccagacagcc catcatcata cgtacttcat
    15061 tcttaatcat ggtcgaacta tctgtcttat ccccaaagaa tcctgctttc ctttctttga
    15121 taaagttttc agcattccct cgttcacgat agaattgaaa cagctcctca ctatctacac
    15181 tgcttaaatt ggtcaaatga acttttttac aaccataaaa aagagcactt aatcgtactc
    15241 ttctctttta aggattagtt gaattaattc aaatccacta gttctcctgt ttctaggtag
    15301 actaggcgtt cacaaatgtt agcaatgtaa tcagcgaagc gttccagatg ccctatgata
    15361 taaaggtatt gtgttccatt gggaattgag gtttcttggt ctttcataag tccaatgatt
    15421 tcctttgata aggcataata atattggtca atctgttcat ctttttgagc aatactaata
    15481 gcttttgagg cttggtgcaa aggaaaggca accaataaat cggctagcat gctgagggat
    15541 aatttaccca tttggtgtaa ctgttcttcg tcaggggcta gttgattttc ttttagttgc
    15601 aaaacagctt tggcaatgcc tgccatatgg tctcccatac gttcaaggtc tgaacaagaa
    15661 gacatgatgc taatcacaaa tcgaaggtca gacacttgtg gctgctgcaa ggccaacaaa
    15721 cgggcacagg tcaattcgat agcgctttga ccttggttga tagcatgatc cttattgata
    15781 attagctctg ccatctcctt gtctttggag gctaaggcca gtaaggcttt tgaagctgtt
    15841 tcaaggacaa gttgccctag tcctaaaaag gattgttcta attcatgcaa ttctaagtca
    15901 aattgatttc tcataggttt ttccttcctt atccgaaccg tcctgaaata tagtcttctg
    15961 tgcgctgatc ttttggattg gtaaacacgt caacggtatc tccaaattcg caaatttctc
    16021 ctgttaagaa aaaagcagtt ttatctgaaa tacgtgaagc ttgttgcatg ttatgggtaa
    16081 caatgataat cgtataatcc ttttttagtt gctgaatgag gtcttcaatt tttaaagtgg
    16141 agatagggtc taaggctgaa gtcggctcat ccattaacag aatatcaggt tctactgcta
    16201 aagctcgcgc aatgcaaagg cgttgctgct gaccgccaga taaggacatg gcactctttt
    16261 taagatcatc tttgacttct tcccaaatgg ctgccccttt taaagatttc tccactaagg
    16321 catctaattg ttttttgtct cgaataccat gtgtccttgg gccataagcc acgttatcat
    16381 agatagacat ggcaaaggga ttaggctgtt gaaaaaccat ccctacacgc ttacgtagct
    16441 gattaaggtt gaatttgcta ctataaatat cttgctcatc taagaggact tggccttcaa
    16501 tatggcaaga aggaaccaaa tcgttcatcc ggttaagggt ttttagaaaa gttgatttgc
    16561 cacaaccaga tgggcctatc aaggcagtaa tctgtctttc tggtaattga atcgaaatat
    16621 tttttaaggc ttgaaaatcc ccgtaaaata agtctaggtg tctgactgaa aatgttccca
    16681 tactaggaag ctcctttcac aagttttcga gataataagc ttgatagagt atttatcatt
    16741 aaaacagtaa taatcaaaat cacaccggta gcataggctt cattgacatg tagcccctca
    16801 cttgacagca tatacatatg cagggctaga gaacgacctg aagacatgag actacttggc
    16861 gtattggtag aggtacctaa tgtatacatg agggcagctg tctcaccaac gatacggcca
    16921 atagctagta tcactccagc taaaatacct ggcatggcaa ctggtagaac aattctaaaa
    16981 acagtccgta acttacctgc cccaagtcca taacttgctt gacgcatgct atcactaaca
    17041 gataaaaggg cttcttctgt tgagcgaata atgactggca acaccatgat gactgaggtt
    17101 aagattcctg ataacagaga gtattgaaaa cctaagaaga ctacaaagaa gagcatgcca
    17161 aacagaccaa aaacaatgga aggaatccca gacaaggtat ctgaggccaa tcgcatgatt
    17221 ttaacacaaa gggaatcttt ttttgtatat tccacaagat aaaaaccagc aaaaatccct
    17281 atgggcaagg ctaaaagaag agcaccaaag accagaataa cggtggaaat aatcgctggc
    17341 ataagggaaa tgttctcaga agtataagtc caagaaaaga gggatagact tagatgaggt
    17401 aagcctttga tgaggataaa accaatgatt aaaaagagag agccaaaggt taaagctgaa
    17461 aaacaataaa cgagaagttt tagcaggtat ttactcataa gatgattttc ctttcaagta
    17521 ggcaaagtag gcattaatca agagaataag gaaaaagaga actgctgagg ttgcaataag
    17581 ggcttcccta tgctgacctg atgcgtaagc catttccaga acaatattgg ttgttaaggt
    17641 tctggttcct gaaaagagtc cacttggaat aatcggctgg ttgcctgcca ccaaaataac
    17701 tgccatggtt tcacctactg cgcgaccgat tcctaaaata actgctgata aaataccaga
    17761 tctcgcagct ggcaagatga cactaaaaat actccgttca tgactagctc ctagagccaa
    17821 gctaccagaa taatacgttt tgggaactgt tcggatagca gattctgaca aactgataat
    17881 ggttggcaaa atcattattc ctaatagtaa cgaagcggtt aggacactca tgccatttcc
    17941 taaaaagctt ctaatccaag gcaccaataa ttgtaggccg aaaaaaccat aaacaataga
    18001 tggaatggcc gccatcaagt tgatagctga ttttaagaag ccatagacgg gctttggaca
    18061 ataataaacc ataaacaccg atgtcaagat gcctgttggc accccaatca caatcgctcc
    18121 taaggtaatt aataaggaac caacgatcat tggtaaaata ccatagcttg ccggaatgtt
    18181 cgttggcgac caatcactgc ctaataaaaa acgggcaaag ccgtagttag ccatgaaagg
    18241 taagccatta ctaaaaataa agaaacagat tagcaaaata gctacaacag ctactgttgc
    18301 actcatgaaa aaaattgccc taaaaactgc ttctttgaag gcttgttttg tcacatcttg
    18361 tcctttctag tgaagaaagt aagggagata cgacacctcc ctacttgcct tctttatctt
    18421 attgtacgat gaaacgtctg catctcttta gagatttatg gagcaaacat tttatttaat
    18481 cttgtcccag gtggttaatt tgccactaaa aacgtctgca agttcagcca tactgacttg
    18541 gcttgcctta ttgtcattat tgaccacaac agcaataccg tctaaagcaa tagcatcatg
    18601 ggtgagactc ttaccttctt caggagttaa ttccctagaa accataccaa tatcagcggt
    18661 tttctcctta acagcggtaa tacctgctga agacccatta gaggtaatat caatcgtaac
    18721 ttctggattt tcttttttat aagcttctgc taatttttcc attaaagaag atactgaagt
    18781 ggaacctaca acagacaact tgcctgataa gtgttggctt gtatattctg tggtttcagt
    18841 tttagcttca ataaatttat tatctgtgac cacttgttga ccttgtttgg agtggataaa
    18901 gctgataaaa tcttgaccta gcttggaaag attagaagac caaacaatgt tgaagggacg
    18961 ttgaagaggg tattcaccat ctaaaactgt gtctcgacta gccttgacac catcaatctc
    19021 taaagctttg acagatttcg ttaaagatcc caaggagatg tagccgatag cattagcatt
    19081 cccttgaact gctgagagaa caccttctgt actattttga atcacagctg ttttggcagt
    19141 gttgtcaatt tttttatcac cgtctttttt gagaatccct gtgatttctg tgaaggcacc
    19201 ccgtgttcca gagccatttt ctcgtgaaat cacctcaatc gttcctgaag ctgactgttt
    19261 ggaagcagct gactgattgc cacaggcaac aagcccaaat cctgataagc caatggctgc
    19321 aagagtaagc atttttttga atttcataat aatcaccttt atctctatgt atttttcttg
    19381 tgtaggctta ctacatttat agtctaacaa gtctttgtaa aggtttatcc ctgattcatg
    19441 taaagattgt gtaaagaatc aaaaaaagcc acttttgaaa aatggctgct cctaaaaata
    19501 gctttaaaaa ttattagtcc tgtgcgaaag attggttagg aagaaaaatc gtgaagcaac
    19561 tgcctctgcc aagctgactc gtcaccgtga cttggccacc taataattga ctgagttctt
    19621 tgacaatggc aaggccaaga ccagtgccac cagtttgtct gcttcgacct ttattaactc
    19681 ggtaaaaacg ttcaaaaata cgatcctgct ctaattgact aataccaatc cctgtatctg
    19741 atacagaaat cttaatgcct tcgttcacct tttgggtctt gacctcaatt tttccccctt
    19801 gttcagtgta acggatggca ttggataaaa gattgagtaa gatttgggaa agtaattgac
    19861 tatctgatac gagggtgaca tcatctggca cctgcacctt tagctgtaaa tccttcttct
    19921 tgagctgagg ttgcaagctt tgagtcaaat cctgtacaaa ttctgccaaa gaaagggtcg
    19981 tccattgtat aggcatttgt tgagccttag ataaggtaag aagatgctca acaatatgct
    20041 caagacgcaa gctttctttg taaataatgt ctagaaagtc atccttgagc gcttcttctt
    20101 cagctgacat ccccttaatg gtttcagcaa agcccttaat cgaagtaact ggtgtcctca
    20161 attcatggga ggcatttgag acaaaggcta aatttaactt ttcataagtt ctaatcgttg
    20221 ttaaatcata tagcaagacg agcacagctt ccacagattg ggtggggcta aaaacgggaa
    20281 ctgctgtcac ttctaaaatc aagtcaccct catgaaaccc acttacttct tgttttaacc
    20341 ttgttttttg atcaaaggct tggtgaacta aatttcgaat atccatccgt ttgaggtcat
    20401 caagtgaact tatgtcgccg tccacatcgg gaaaataatg aggcagagag cgactggata
    20461 ataacatctg accttgagcg gaaactaaaa acgtccccat ggttaggtgc gacagaagaa
    20521 cctccattgt ttcggctagg tccttgtatt gctgatcctg ttgggagact ttggttttta
    20581 gggcagacac atactgagcc aaagacttta agtcttcttg ccctttttct aaaaagtatt
    20641 cactactggt caagagaggt tggtgcaagg tctcaaaagc aacttcccat ttccaaaggc
    20701 aaaagagcca gtagccacct tgtcccaaag aaagggctag aagaaagaga ccgatgcctt
    20761 tactgatcca agttaatgcc atccctgcaa tcagaatgag gctaacactt agattgacta
    20821 gccaaaattg aaggtagcgt ttcatctata actccttgaa cttataacca taaccccgaa
    20881 tggttcgaat aaattgaggg gctttaggat tgtcttcaat tttttccctc aacttaccaa
    20941 tatgaacgtc caccaaacgt gtttcctgcc caaagtcata cccccagata cgttccaaaa
    21001 gacgctctct agtcagtgtc atgttgggat gtttcataag atagagcaag agttcaaatt
    21061 cttttggggt caaactcagt aacttattcg ccttgtagac ttcatgacgc tcagggtata
    21121 ctttcaaggt cccaaatagc caagaatcgt cagcgatatt atctgaatca tctccttctt
    21181 gttctccttt agttcgcctg aggacagcct tgacacgcgc cagcaattct ctagggctaa
    21241 aaggtttggt caggtagtca tcagccccta attccaaggc caaaacctta tcaaattcat
    21301 cacttttcgc agaaaccatc ataattggag ttttgacgcc tttggctctc agccgcttac
    21361 aaacttccat gccatctaat tgtggtaaca tgatatcaag caagataaaa tcaaagggtt
    21421 ctgtttctgc caaagctaag gccttccgtc catttgttac caattgagta gaaaagcctt
    21481 ccttacttaa atggtagtca agcaatttca gaatgtgttc ttcatcatcc actaataaga
    21541 cttgttttgt catctattat ctcctattgg taacattata acacaattat cagaaatcct
    21601 aacattgcta aatcagatta aatttgctta tcaagactag tatctggtca aacgctcaat
    21661 catctccttg tgctctggat aggtcgccag tagatctacc ctttcaaata attcaaaatc
    21721 ctcaaattca aaaccaggag caacaagaca agaaaccaga gcatcatcct tatcaactgt
    21781 tgatccccaa atagtgccct taggaacaca gtagtgaagt tgttgccctt tggatatgtc
    21841 caggcctaaa gtgactgctt cgtagtgacc atctgctgta atcatgtgaa cagtaagtgg
    21901 ggattctgca tgaaaatacc agatttcatc tgctgtcaat cggtgaaaat gtgaaggatt
    21961 cgtttcttct taataagaaa taaatactgg tataaagcgc ccttccctta ccagcaaggt
    22021 ttatagtgtc tgaagctttt tttgtttgtc taaaatagcc accttcaaca tggggagcta
    22081 actctagagt tcttatcaag tcttctttat ccgtcggagc caatgggttg aagtaactct
    22141 tgttcaaagg tggtgttacg atttcaagaa ctcctctcag ctctgaggac acgataatga
    22201 ttgatgcgac ggaagtaaaa atcaatcgcc ctaaaaaaac aattagcgaa taattctggt
    22261 aaaaagaaat ttcacgctat gaaggctcaa gcgattgtca caagtcaagg gagaattgtt
    22321 tctttggata tcgctgtgaa ctattgtcat gatatgaagt tgttcaaaat gagtcgcaga
    22381 aatatcggac aagctggtaa aatcttggct gacagtggtt atcaagggcc catgaagata
    22441 tatcctcaag cacaaactcc acgtaaatcc agcaaactca agccgctaac agttgaagat
    22501 aaagcctgta accatgcgct atctaaggag agaagcaagg ttgagaatat ttttgccaaa
    22561 gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg cttcggatta
    22621 cgaatgaatt tgattgctgg cattatcaat catgaactag gattctagtt ttgcaggaag
    22681 tctattattt ggttaggtga attagtgaag cgtttaggca agtgtctctg gttacgacgt
    22741 catggcctct aaatcgatta tatttagggg tcatgactag tgaagcagtt agctagttcg
    22801 catataagcg gctagcgtct aacaattagg aactttagtt ccaataactt taagattacg
    22861 acgttttagg acataaatcg atcatattta tgtcctaaaa ctagtgaagc gcctagccaa
    22921 agtccgaata ggatttggcg ttagttactt agattgcttt gcaatcaagt aactttggcg
    22981 atttacatct tctctggcgc ttctactcca agcaagcgaa gggcttcttt gagaacgact
    23041 gcggttgcgt agctgagggc tagacggctg tcgcgttctg ggctttcatc caagatacgt
    23101 gtatgtgcat agtatttgtt aaaggattga gccaggctaa ttgcaaattt agcaatgata
    23161 gaaggttcaa agttatctgc cgcacggttg ataatacgtg ggaagtcttg aatgagttta
    23221 atgatttccc agctttcagt atcattcaag ctatagttgc cagctgtttc tggtttgaaa
    23281 tcggctttgc gtaagataga ttggatacga gcgtaggcat attgaacgta aggtccagtt
    23341 tcaccctcga aggataccat agcttctagg tcgaagtcgt atccatttgt acggtcggtt
    23401 ttgaggtcat agaatttaat ggctccaacc ccaacagcat gtgctacttg gtctttgttt
    23461 tctagttcag gatttttagc ctcgatttgg accttggcac ggctaacagc ctctgcaata
    23521 gtaggctcta gcaagatgac attcccttta cgagtagaga gcttcttccc ttcttttgta
    23581 accagaccaa aaggaacgtg ggtaatgtcg tcactccaat cgtagcccat ctcttgcaag
    23641 acagctttga gctgtttaaa gtgggcagat tgttcttgac caacgacgta gatagattta
    23701 gcaaattggt attcattttt acggtagagg gctgcagcca agtcacgtgt gatatagaga
    23761 gttgcaccat cagacttctt gatgagggct ggatgttcaa ttccgtattt ctcaagattc
    23821 acaacttggg caccttctga ttcaagaagt agtccttttt cagaaagaat gtctacaact
    23881 gcatccatct tatcattgta gaaggcttct ccgttatagc tgtcaaattc aaccttcaat
    23941 tcattgtaaa ggcggttaaa ttccactaaa ctttcatcgc ggaaccattg ccaaagagcg
    24001 agagcttcct catctccatt ttcaagttta cggaaccatt cgcgcgcttc ttcatccaag
    24061 ctagggtcat tttcagcttc agcgttgatg cggacataga gtttaaggag ttcatcgatt
    24121 ggatgagctt ttacagcttc ttcgtcgccc cattttttgt aggcaacaat caacatccca
    24181 aattgtttac cccagtctcc caaatggttg accttgaccg tttgataacc gattttttgg
    24241 aaaatatgtg acaagctatc tccgataaca gttgaacgca ggtggccaat agaaaatggt
    24301 ttagcgatat tcggactaga catgtcgata acaacatttt cttgtttacc aatattttgg
    24361 tcagcatagt gttctttttc agtggtaaca gcttgcaata cttgagcaga aatggcagat
    24421 ttatcaagga aaaagttaac gtaaggtcct gttgcgacaa ctttttcaaa ggcttggctg
    24481 ttcatttttt cagccagttc agccgcaatc atttgtggtg ctttacgttc gacttttgca
    24541 agagaaaaag cagggaaagc aatgtctccc atttctgagt ttttaggggt ttccagtaac
    24601 tttaaaatag cctcttggtc caggctatca atgatgctag ataatttgct agcaatcaat
    24661 tcttttgtat tcattaagag ctcctttttg gacttttcta c
//
LOCUS       DAVLSL010000016        23489 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLSL010000016 DAVLSL010000000
VERSION     DAVLSL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.016"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(33..1364)
                     /gene="hk06"
                     /locus_tag="VXN30_000521"
     CDS             complement(33..1364)
                     /gene="hk06"
                     /locus_tag="VXN30_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1357191.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(1361..2014)
                     /gene="rr06"
                     /locus_tag="VXN30_000522"
     CDS             complement(1361..2014)
                     /gene="rr06"
                     /locus_tag="VXN30_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1357192.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(2335..4767)
                     /locus_tag="VXN30_000523"
     CDS             complement(2335..4767)
                     /locus_tag="VXN30_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1357193.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(4769..5227)
                     /locus_tag="VXN30_000524"
     CDS             complement(4769..5227)
                     /locus_tag="VXN30_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1357194.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            complement(5346..6074)
                     /locus_tag="VXN30_000525"
     CDS             complement(5346..6074)
                     /locus_tag="VXN30_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357195.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(6074..7081)
                     /locus_tag="VXN30_000526"
     CDS             complement(6074..7081)
                     /locus_tag="VXN30_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1357196.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(7120..7878)
                     /locus_tag="VXN30_000527"
     CDS             complement(7120..7878)
                     /locus_tag="VXN30_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1357197.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            complement(7841..8131)
                     /locus_tag="VXN30_000528"
     CDS             complement(7841..8131)
                     /locus_tag="VXN30_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1357198.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVPMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(8202..8304)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8387..9733)
                     /gene="cbpD"
                     /locus_tag="VXN30_000529"
     CDS             complement(8387..9733)
                     /gene="cbpD"
                     /locus_tag="VXN30_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1357199.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(9822..10088)
                     /locus_tag="VXN30_000530"
     CDS             complement(9822..10088)
                     /locus_tag="VXN30_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357200.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(10090..11442)
                     /gene="dnaB"
                     /locus_tag="VXN30_000531"
     CDS             complement(10090..11442)
                     /gene="dnaB"
                     /locus_tag="VXN30_000531"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1357201.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(11486..11938)
                     /gene="rplI"
                     /locus_tag="VXN30_000532"
     CDS             complement(11486..11938)
                     /gene="rplI"
                     /locus_tag="VXN30_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1357202.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(11935..13908)
                     /locus_tag="VXN30_000533"
     CDS             complement(11935..13908)
                     /locus_tag="VXN30_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1357203.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(14044..14592)
                     /gene="raiA"
                     /locus_tag="VXN30_000534"
     CDS             complement(14044..14592)
                     /gene="raiA"
                     /locus_tag="VXN30_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1357204.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(14672..15334)
                     /locus_tag="VXN30_000535"
     CDS             complement(14672..15334)
                     /locus_tag="VXN30_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1357205.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(15331..16629)
                     /locus_tag="VXN30_000536"
     CDS             complement(15331..16629)
                     /locus_tag="VXN30_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1357206.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLSRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            16685..17320
                     /locus_tag="VXN30_000537"
     CDS             16685..17320
                     /locus_tag="VXN30_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1357207.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYASSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            17605..18525
                     /gene="cysK"
                     /locus_tag="VXN30_000538"
     CDS             17605..18525
                     /gene="cysK"
                     /locus_tag="VXN30_000538"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1357208.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18722..19120)
                     /locus_tag="VXN30_000539"
     CDS             complement(18722..19120)
                     /locus_tag="VXN30_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1357209.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            19111..19398
                     /locus_tag="VXN30_000540"
                     /pseudo
     CDS             19111..19398
                     /locus_tag="VXN30_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19553..19876)
                     /gene="tnpB"
                     /locus_tag="VXN30_000541"
                     /pseudo
     CDS             complement(19553..19876)
                     /gene="tnpB"
                     /locus_tag="VXN30_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(20122..21162)
                     /locus_tag="VXN30_000542"
     CDS             complement(20122..21162)
                     /locus_tag="VXN30_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1357210.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            complement(21241..22020)
                     /gene="rpsB"
                     /locus_tag="VXN30_000543"
     CDS             complement(21241..22020)
                     /gene="rpsB"
                     /locus_tag="VXN30_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1357211.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRTRLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            complement(22244..23422)
                     /locus_tag="VXN30_000544"
     CDS             complement(22244..23422)
                     /locus_tag="VXN30_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1357212.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
BASE COUNT         6891 a         5273 c         3997 g         7328 t
ORIGIN      
        1 cctatgtgaa ctttaataaa aaactaatct gtctacaagc taatcttaaa ttccataccg
       61 tgttcataag ggagaaaact atagtccatt tgatagcttt ctaaaatatt actcacaatg
      121 taaagaccca aaccggaact ttcatcctta tctgtcacga tttgagaatg atagaatata
      181 tcaaataaat gttctagttc ttggtcactt agaggcgcac atgtattttt gatagagaga
      241 tagccatctt gctcggatat gattacacgc ccatttttat ctgaatattt aattgcatta
      301 ctaatcaggt ttgtcaaaac cttatccaat gccctaagac tcatgacgac cctggtagca
      361 tctgtcaatt gattttcaat tgtaacacct attgaatgag ctaattcttg ataacgtgat
      421 aaaatatcta ctaaaactgg cttaacagtc aaggtctcac gacactctgt ccactcttgg
      481 aatttagaag actccagtac ttcttccaat aagtggctca tctggtcaat cttatttata
      541 ctctttgcaa tatattttgg atgatctttg taatctccaa tattatgctg catattctct
      601 aggataattc taagactggc taaaggggtt ttcaactcat gtgatgctcc gcggacaaag
      661 gaaacctttt gattttgcaa ttttacaatt tgctcattac gactttccaa ctcatgaata
      721 accttcaaca agtgctcata cataccatta atctgttttc caacttcacc aacttcatct
      781 ttcctacttt catcaaaacg aatattgtca tccaaatctt gcattttact agtcacttct
      841 gaaatgtaaa aaagaggatt gagcaagcgt ttagtataaa aataagaaaa aacaaaggaa
      901 aaagcaattg taaccaaaaa tgtatatggg agatacaaaa gcaaaatatt ctttgcttct
      961 ttgtagacat ctaccccgtg aacaaattgc acggttacct gtttaccatc tgctgtacta
     1021 acagacatat ctaacatcac gatataattt gatagctttc catctttaat atccaagtca
     1081 tgcacaagag ggagccgctt atctacaata tctcttttaa ggtgagcaga tatggtcaag
     1141 gactttgaat aagactggag taagcttgga atttcatcag atgtcttagt ctttaaatac
     1201 tccgtaaaca ctcttgcgct ctcattaaac ttttccccct ccagttgaat ataataaaaa
     1261 ggaaaggtca aataaatagc tatatgaatg actagaccaa caccacctag aattgcaaaa
     1321 cttcttaaaa aagacttggt taataattta ggatttttta tcataagcta atcttatacc
     1381 caacattttt cacagtcacg atacaatcta aagctaattt tttgcgaaga gtacgaatat
     1441 agacatcaac gaccctatca caaggtaaat cagctacatc ttttgaaatt tcttccaata
     1501 tctgctctct acttaaaact tgatttttat gctgaatcaa acactttagt accagtaatt
     1561 cctttggttt gagatcaatt tcttcatttt tataatgtgc tttgtaagag gtaaaatcca
     1621 ctgttacatc ctgatatcgc caaagatcct ctatgacgta gtaacgtctg ataagcgcct
     1681 taatacgctt gacaagcagt accaaagaaa aaggttttgt cacatgatca tcagcataga
     1741 gatcaaatac ctgtgactga gtagtttcat ctcccagagc actcaagacg atgacaggaa
     1801 tatcgtgctt ttgattgatt tcttttaaca cttcaaaacc actcttccta ggcatcatta
     1861 aatccagtac catgagatgg atagggagat cttgaaattt ttccaagacc tcttgtccat
     1921 ccttagccat aatgacatga taaccctctt ctgtcagaaa tgctgcaatt ccttctctaa
     1981 tcatttcctc gtcatctgca actaaaatgt tcatctctct ccctttctac taccagaact
     2041 ccatacatct atttcctgta tgctatttga aaatttccta ccagcctatc atctatgata
     2101 atggaatgga tttcatttat ccgctacttt ttgtcaacaa caacgtcaat acaagaaaat
     2161 cattcatctt gcagcttata ctcttcgaaa atctcttcaa accacgttag ctctatctac
     2221 aacctcaaaa cagtgttttg agcaacctgc ggctagctac ctagtttgct ctttgatttt
     2281 cattgagttt cactccactg tgacttatgg tcagatgctg gtacttttat tcttttatgc
     2341 aatatcaaat tttaactggc ctgctttgac accaatctta agtgtgctgc ctgccactaa
     2401 atctcccttg agaagaagtt ctgccaactt gtcctccact tctgtttgca gggttctgcg
     2461 aagtgggcga gctcccatct ctgggtcata tccttgattt gctaacaatt tcagagctga
     2521 agcttgtaat ttcaagtcaa tgcctttttc agccaaactt gccactaaag gcttgaccat
     2581 aatcttcacc acttcctgca tatggtcact agataggcta tggaagacca ccttctcatc
     2641 aatacggttg atgaattccg gtctataagc ttttttcagt tcttcaaaca tgcgtttttc
     2701 catattttcc tggtcaaaac gaatatcctt agccccaaaa ccaacagtct tatcatcacg
     2761 aagggcagtc gcacctagat tcgatgtcat aatgataatg gtatttgaaa aatcgacctt
     2821 gcgtcccttg ctatctgtca agacaccgtc atccagaacc tgcaagagaa cattaaagat
     2881 atctgggtgg gccttctcta cctcatcaaa gaggagaacg gaatagggtt tattgcgaac
     2941 cttctctgtc aactccccac cttcttcgta acccacatag cctggaggag ctccatttag
     3001 acggctggct gcgaatttct ccatatactc actcatatca aagcggataa gggctgattc
     3061 gtcatcaaag agaacttctg ccaaagcctt ggccaactcg gtcttaccga cacctgtggg
     3121 tcctaggaac ataaaggaac cgatcggacg cttgtgactg cgaattcctg actgattgcg
     3181 gcgaatggca cgactaatac ttgaaacagc ttggtcttga ccgatgacac gtttgtgcag
     3241 ttcagcttcc aagtttaagt atttcttggc atcagtctga gtcagttttt gaacggggat
     3301 acctgacaag cgactcaagg tggtcaaaat atcagactct gtcaccaagt ctttatagac
     3361 aggcacttcc tcttcttttg cgattagctg ggctgcctgt ttccacttgc catccatcaa
     3421 ggccttgtca gctggactca agtcagaatc gtctgctttt acatgctttg atttattttg
     3481 cactgttgct gccgcctcat ccaagagatc gatagcagag tctggcaagt gacgactggt
     3541 taaataacga tgagccatct taaccgctgt ttcaaccgct tcatctgtga tttgtacacg
     3601 gtgatgtttc tcataagtcg ccttcaaacc ttgtaaaata gtcatactat ctgccacact
     3661 tggttcttca atcgtcactt tagcgaaacg acgagaaagt gctgcatctt tttcgatatg
     3721 tttttgatat tcttcctgag tggtggcacc aaccgttctc aaagttccac gcgccaaggc
     3781 tggtttcaag atattggccg catccagagt cgaatcaatc ccgctaccag aacccatgat
     3841 ggtgtgaagt tcatcgataa agaggatgac ttggccatct tcttcaatat ccttgatgat
     3901 attattcatg cgttcttcaa agtcaccacg gaagcgtgtc cctgcaacga cattcatcaa
     3961 atcaagttct aacacgcgca tcttagccat ttccgcaggc acgtcaccac tagcaatacg
     4021 ctgagcaaga ccaagcgcca gagctgtttt cccgacacca gcatccccaa ccaagacagg
     4081 gttgttctta gtcttacggc tcaagatttg aatcatacgt gagatttcct tgtcccgacc
     4141 gatgactggt tctaacttgc cagaacgcgc ttgctctgtc aaatcatgcg tatagtcctc
     4201 gagaccacca ctaggagtct gcggcatgcc catcatattg gccatagaat tttgcttgtc
     4261 agctactgta cgatggcgtt ggcgtaaagc cttgagatct tcacgagtcc agcctgcccg
     4321 ttcttctaaa tttcgacgaa gagcagcaat cttgacctga tctttcttgt cttcataaga
     4381 aaaaccagcc ctctccaaga tacgagtcgc caaggcattg ccatcatgca aaatcgcata
     4441 gaggacgtgc tctgtcccta gtaccttagc atggaccact gacgctacat actctgcttc
     4501 atcaaaaaga acctgcaaac gacgggagaa cggcaattcc gtaaaggttt catcctggct
     4561 atagtccgtt tcagtcagtt ccaaagccac ctcttctaaa cggtccatct catacggata
     4621 atcatttaaa gttgcccctg ctacactata actgtgatta gacatggcaa tcaacaagtg
     4681 ccacgactct agataacgag ctccaaaatg tccagcaacc atgtaggcac tttcgataca
     4741 ttcattcaat gcttttgaat agttcatctt acttcccttt tctatctacc tcttgtatga
     4801 tctgacgtag catgtttgct cgaacaactg gagcttcttc tcctaaaacg cgatccaaag
     4861 ctactgattc tagcaaattc atctcctgct tggtcatcaa ttcctgctca accaaaagct
     4921 ggagaatatc ctcataaatt tcttgactga ctcgctcacc aatcgagtaa agcagctccc
     4981 ggagcatttc atgatgacta gaaaactcaa ttcgtcctat acgaatgtag cctccaccac
     5041 cacgcttact ttcaaccaag tagcctctac tttccgtaaa gcgtgtcttg atcacgtagt
     5101 taatctgact aggaacaacc tgaaaggtat ctgccaactg actccgttgc aactccacga
     5161 taccagattg atctaaaatc gccttgatgt aggcctcaat atgatccgat gtatttttaa
     5221 atctcataac aaacccacct ctctctttaa accttgacct tgtttgacta ttataccgaa
     5281 attttctcat ttttaaaaga aaaagggcgc tggtaaagga taatcttcac caactcccta
     5341 tttttctact tatctaagcc taattctgcc aagatttgac gtttgtaggc aatcttttgg
     5401 acttccttat cctcatcttc agaccaatct agtttaattt ctgaaacaat ctgcccaggg
     5461 cgatttttca agatatagat gcggtcgctg agattgaggg cctcctcaat actatgcgtg
     5521 atgatcaggg ttgttagctg caactgcttg tgaatctcaa gataccaagc gtggagttcc
     5581 atctttgtca tctcatccaa ggcgctaaag gcctcatcta agagaaagag cttgtgccca
     5641 aaaaggtagg tccggagtaa ggctacacgc tggcgcatcc caccgctaag ttcatgagga
     5701 tacttgtctc ttacagctgt cagctggaag gtcgcaagaa ttttatccgc tcgggaaata
     5761 gcttctgcct tatccacctt ttgaatcaag aggggcagaa tgatatttcc aagcaccgtc
     5821 ttgtgctcca agagcagatc cttttgcaac atataactca cgcgcccctt gggattttct
     5881 tcaccatcaa ggacaattct ccctgactga acttctaaaa tcccagcgat tagattaaag
     5941 agggtggtct ttccaacacc acttgggcct aggatggaaa ccacttcgcc tgaagtcacc
     6001 tgtaggttga tatcctctaa aatcctctcc tgaccatagg cataactgac gtgctctagt
     6061 ctaatttctg tcattatttc acaaattcgt tggtgaagcc tttgtctgtc aagtcttctt
     6121 taaggatacc attttcttta tcccatttgt agaaagcatt ccagcgagct gcgtcaaatt
     6181 gaccccattt ttccttgtcg cttgcgtatt cttttgacaa gtatttttga gattcgatga
     6241 caaagtcacg tttttccttg agttcaggtg cattcttgat gagaatatct gcagcttctt
     6301 ctggatgttc catggcatat tggtagcctt ttttgatggc ttggatgact ttgcgagctt
     6361 cttctttgtt atctttcaga tagtcgttat ttgcgatgat aactggtgaa tagtagtcaa
     6421 actccttgac atagtctttc aagtacatga agttagcatc tacaccttga gatttagcaa
     6481 ggataccatc ccaaccgtag taaatccaag cagtatcaaa gacgccattg gcaatcggtg
     6541 tgattgagtt tgagtcgtta tttggtactt tttcaacctt ctcaaagtct ccaccttgag
     6601 attctaccaa ggttttcaac atagcaagtt cagttgggtc attccatgtc ccatatttct
     6661 taccaaccaa gtcttttgga ctgcttacat tatcagattt acgagagatg attcctgatg
     6721 tattgtgttc aacaatagct gcaacggcag tgattcctgc tcctttttcc aatttcttag
     6781 ccatgtagtc ttggaaatac actgcaaatg gtgcctttcc gttgataacc aagtcagaag
     6841 aactttcttc tggtggcaat ttcaaatcaa catccactcc agcttctttg aaataacctt
     6901 tttccttggc aacataaagc cctgtgtggt tggtatttgg tgtccagtct aggataaagt
     6961 caaccttctt aagttctgcc tctttgttgt ctttagaagc agttccttga ccacaggcca
     7021 caagcacaac agctacaaga gctgttacaa gcgttaaaaa cactttccat gttttcttca
     7081 ttttgatttc ctcttttctt tttcagaaac attctaattc tacgaacgtt tccatttaat
     7141 aacatatttt tcactaatat cgaccaactt catacccaaa aggctgataa tcgataccag
     7201 aataataata gcgaacatgg tatcatactg aaacaatttc ttggactgaa tcatataaac
     7261 accaagcccc tcaaagcctc ccaaccactc agataccact gttgtgataa aagcgtagga
     7321 gacactgacc ctcagaccag cataaaagta gggcaggctg actggaattt taaaatgcca
     7381 caggatttgc caaggcttgg cccgcatcag actaaacaaa gtcagcatat ccttgtcgca
     7441 atgcctaaaa ccgtccaaaa tgctgacgat gatgggaaag gttgtcgtta agataatcaa
     7501 gacaatcttg ggcaaaatcc cataacctag ccacaagacc aggataggag ctatggcaat
     7561 ggtcggaatg gtctgaatga ccaccatcat agggtaaatc aggtcattga gccaagtcaa
     7621 actatccatg agtacagcca taagacaggc aatcaaaact cccaaaatca gccccagtaa
     7681 agccactctc aaggtcgccc agctatggtg ccagagaaat tctctgtcac gaacaaaggg
     7741 ctggagaatt tcaagaggtg tcggcaggat aaacttgggg agcagtttaa gaaaacctgc
     7801 taactgccag attgacaaga ctccgagaaa gcccaataga ctaatgtgtc gtctcagtat
     7861 acttctcaag tttctcatca atacttaaaa tctctcctac atttattttg acattggcaa
     7921 agacattgtc tgcctcctgc cctgccactt ccagcgcttc ttttagaatg cgcataagct
     7981 catcaaactc cccttccaag accgtttcaa atggtgtcac taccatcggc acttcttgag
     8041 tttgcagata agcaatgacc tgatcaataa cagctatccg atcaatcccc tgtactaggg
     8101 gtaaaacttg caaggcaatg cttgctttca taagaacctc ctcttctcgt tttcctttta
     8161 aaaaaaagaa aaacctttac catacatgga aaaggtgcaa aattagacta agtatcgttc
     8221 cctccgctgg cattacccag atcaggtgcg gtcgaagttt aacacttcct ctcagactgt
     8281 acacagactc ccatatatta tatgtatata tgatagcgca aaataagaaa aatgtcaagg
     8341 aaactgctta cagaaaagag caggaaattt ctcctactcc aattttctat actcgttctc
     8401 catcactgtt tactctgtag ccatccaccg tcgtattaac agctaaggcg cctgaagagt
     8461 aagaataata ccacttacca gagacctggt accagcctgt tttcattgcc cctgaactac
     8521 ccagataata ccacgagcca ttctccttta accatcctgt ctgcatttca ccagataatt
     8581 tcaaataata ccagctagaa ccatctttca gccatcctgt tgatttagaa ccatttgatt
     8641 tgaaatgata ccaactacca tttattttct tccaaccgac agttgaacta ttattggatc
     8701 catagctaga gatatttcct gattgattct gataattttg tgaagaagtc actccctcta
     8761 gctgtatata gcgacgactt ccgttataag ccgtataact caaccacttg tagccgtctt
     8821 tttcaagtat ctgatcataa tgaaccttct ccccaggata atagtaatca atcacagttg
     8881 cactaactag gggttcagtt ttgatagcag acttggtctt aaaataatga gttccacctg
     8941 ttgaagaaag aacagaatta cctagaggat ttttattcac agcctccaat tgaacataac
     9001 gatagcttcc attataggca gtataactca accacttgta gccgtctttt tcgagaattt
     9061 gatcataatg aaccttctct ccaggataat agtaatcaat cacagttgca ctaactaggg
     9121 gttcagtttt aatagcagac ttggtcttaa aataatgagt tccgcctgtt gaagttgagg
     9181 attgactatt cccaacactg ccaccatcca aatctttaaa atgaataaat cctgtcatcg
     9241 tgtttgcttt tataattcgt ttattatagg attctgtata accatagtta tattcctcaa
     9301 tctcaatctg atctcccatt acatttgaca cccaggcaac atgaccataa gttcctgcag
     9361 tagaccaagt aatggaacca atcgtcggtg tattatctac acgataacct tcccgacgag
     9421 cacgatgtcc ccattcattc gcatttccat aagctgccgg aatttcaaaa ccattgacat
     9481 tactcaaacg aaaggctaca aaagaagtac actgacgaga atacatgcgc cactgatcaa
     9541 tctcctggct cccattttta taataagcag gataatcatc cccacgcgca atcgatccat
     9601 ttcctccgga ataggcatag acactatcac cagctgctag catcaatcct actactaaaa
     9661 aaggaacaag ctttttaact gacatcttca agtaataact tgttcctctt gctataaacg
     9721 gtaaaatttt cattcttcct ccttaaaaaa taatataaag cgatgattgc cttcacctaa
     9781 attattcgga gaaaaaagaa aaagtgagaa aatttctcac tttagtcaag ataatgaatc
     9841 aaattctttt ctgtaagaat atctgagtaa gtaaaggatt caacgtaaac attaacttgt
     9901 ttatctcctt ccacatcccc aaactccaca ataaataggg atggataaac ttcaattagc
     9961 ttacccaatc tatttttttg gcgcttacga ccattctcca aagtcatttc tacgacttgt
    10021 ccctcatgtg ccttgatttc ttctttgatt tttttcatct tggctacatc tgtaaatgca
    10081 tctgacatct tatgcctccc tctttgagat acttgaaaat ttattgtatt ctttttggaa
    10141 aatcaattcc actgttccac gagctccact acggtttttc tcgataataa cttccacctt
    10201 attatttggg ataccctcct cttcttcacc accacgttca tagtagtcat cgcgatagag
    10261 aaaagctacg atatcagcgt cctgctcaat agacccagat tcacgaatat cagacaagac
    10321 cggtctcttg tcctgacgtt gttctacacc acgagaaagc tgactcagag cgattactgg
    10381 aaccttcaat tccttggcta gtattttcaa ctgacgagaa atttcagaaa cttcttgttg
    10441 acgattttct cgaccagttc ccgtgataag ttgcaaatag tctatcaaaa tcaaaccaag
    10501 atttccagtt tcttgagcca atttacgaga acgagaacga atctctgtaa tccgaatacc
    10561 tggcgtatca tcgatataga tactggcgtt agctagatta ccctgagcaa tagtatattt
    10621 ttgccactcc tcatctgtca attgccctgt acggatagaa tgtgactcca ccaagccttc
    10681 tgcagctaac atacggtcta ccaagctttc cgcacccatt tcgagtgaaa aaatagcaac
    10741 cgttttgtcc aacttagtcc caatattctg agcgatattc aaggcaaatg ctgtcttacc
    10801 aactgctgga cgagctgcta agataatcaa ctcctcctca tgaagtcctg ttgtcatatg
    10861 atccaaatca cgataacctg tcgcaatacc tgtaatatcg gtcgtttgtt gcgagcgagc
    10921 ttccagattt ccaaagttga gattcaacac atctcgaatg ttcttaaacc cgcttcgatt
    10981 tgcattttca ctgacatcaa tcagcccttt ttctgcctga gcaataattt catcagctgg
    11041 ttgtgacgct tcgtaagctt ggttgacaga ctctgtcaac ttggcaatta aacgacgtag
    11101 cattgctttt tctgcaacaa tcttagcata atactccgca ttagcagaag ttggcacaga
    11161 attgacaatc tcaaccaagt aagacaagcc accaatattc tgtaaatcac cttgattatc
    11221 aaggatagta cgaaccgttg ttgcatctat ggcatcacca cgatcggata aatcgaccat
    11281 ggcttggaaa atcaaacgat gggcatactt aaaaaagtcc cgagactcaa tgtattctcg
    11341 cacaaaaaca agtttactct catcaataaa gatagcccct aaaacggatt gctcagctaa
    11401 gatatcttga ggttgtactc gtaactcttc tacttctgcc atcagacttc ccttcctttt
    11461 acaatcttgt caagaaggtg taaacttatc cttctttcac acgaagattg attacacttg
    11521 tgatatcttg atagattttc actggcacat caatcaaacc aaccgctcga atcggagctt
    11581 gtacttgaat atgacgttta tcaatcttaa ttccaaattg cttttgtaat tcttctgcaa
    11641 tcttcttatt ggtaatagaa ccaaaggtac gaccatctgg accaactttt tcaacaaatt
    11701 ctacaacagt ttcttctgct tcaagttgtg ctttaattgc tttcccttct gcaatcatct
    11761 cagcgtgagc tttttcttcc gatttttgtt taccacgaag ttcacctaca gcttgagcag
    11821 tcgcttcttt ggctagattc tttttgataa gaaagttttg cgcataccct gttggtactt
    11881 ccttaatttc gcctttttta ccttttcctt taacatctgc taaaaagatt actttcattc
    11941 ttctttctcc ttttccttta tttcatttaa tacaatttct gtcagttttt cacctgcttc
    12001 tgacaaggtt acatctttaa tttgagctgc tgccaaatta aagtggcctc caccgcctaa
    12061 ctcttccata atccgttgta cattcagttt actacgactt cgagctgaga tagagataaa
    12121 tccttgtgta ttcttcgcaa gaacaaaact cgcttcaata cctgacatgg ctaacatggc
    12181 atctgctgcc ttactaataa caactgtatc atagcatttc gtgtccttag cctctgctat
    12241 tagtacatct gaacctaatt tacgcccctg taaaataagt tcattgacct cacgatattc
    12301 ttcaaaatct gtcgcagcga tttcctggat agcaatacta tcacttccgc gcgttctgag
    12361 atagctagca acatcaaatg tccgactagt tactcgcgag gtgaaatttt tagtatccaa
    12421 catcatacca gccatcaaga cacttgcttg catacgactc aaacgatttt tcttagaatt
    12481 ctggaactga atcaattccg ttaccaactc actggcacta cttgcaccac tttcgatata
    12541 agtaataacc gcattatctg gaaaatcctg atcccttcta tggtggtcaa taacaatggt
    12601 ttgggtaaat aaatcataaa attcttttga taatgttaag gctgtctttg aatggtctac
    12661 aagaatcaac aaagaacgat tggtcaccat ccccattgca tccttaacag acaacaactt
    12721 cgtaactcct tctttttcta tgaatgaaac agctcgttca atatctggag acatttgttc
    12781 ttcatcataa agagcatagc tattttcaat cacattgctg gcgaacaact gcatacctac
    12841 agcagagccc aaagcatcca tgtctaaatt tttgtgaccg actacaaaaa catgatctac
    12901 actccgaatc ttatctgaaa tagctgtcat catagcgcgc gtacgagtcc gtgtacgctt
    12961 gattgaagca gcagacccac caccaaaata aactggattt ttcgtttcgt cgttttcctt
    13021 aacaaccacc tggtcgccac cacgtacttc agccaagttc aaattgagca aagcaacttt
    13081 ccctatctca tcatgatttc catcgccata agaaaatccc atacttaagg tcaagggcaa
    13141 ctgtctctgt ttcgactctt ctctgaaagc atcaataaca gaaaatttat cattcatcaa
    13201 gccctcaagc accgtgtagt cagtaaatag ataaaatcga tccatactta cccgacgaga
    13261 aaacatcatg tatttttctg aaaactctga tataaaatta gctacaaaac tattgatttg
    13321 actaatatct gactcagaag ttgcatcctc caaatcatca taattatcca cagagacaat
    13381 cccaatcact ggtcgacttg ttaccaattc atctgttatg gcttgttccc tggatacatc
    13441 tacaaaatac aaaacaccgg aagaagcatc catatgaaca gcataacgct tctcaccaag
    13501 cttggcataa gtagacggat ttcctactga agccttgata atcgtttgaa cagcttctaa
    13561 atcaaaatca ccatcttcct tggtcaaaat caattcagca tagggattaa accactcaac
    13621 ctctccagaa gataaattca atttcataac acctacaggc atttgttcca atagagatgt
    13681 caaactttct tccgcttggt ggtttacata ctgtatctgt tctacatcac tccttgtata
    13741 atgcactctc agtttcttaa ataaaaaaac atagcctcct acaaaaagaa acaaaattaa
    13801 aaccgtcaac agattattat taacaaaaat aatgaaagtg gataagactc caaacgcaat
    13861 caatcctact agaataggaa aaattggact tacataaaat tttttcattc aaaacctctt
    13921 ggcacccatt ataccataat acccctcaaa aagcgacttt ttaaaagtgt aatcagtaat
    13981 tctatcaatt ataagaaaaa ggtagtttac aattcagtaa acctaccttt acacatattg
    14041 aaattaagat tctttaacct ctaacaaacc aatttcgcca tcctcacgac gataaatcac
    14101 attggttgtc tgatcttcaa catccacata gataaagaaa tcatgcccca ataaatccat
    14161 ttgtagaatt gcttcttcca aatccattgg ttttaaatca atttgttttg aacgaacaac
    14221 tttagactgg acaacatttg aatcttccac caaagcatct gtaaataatt gaccagttgc
    14281 taccttattt ttatttttac gctcgatttt tgttttattt ttacgaatct gacgttcaat
    14341 tttatcagtt acaaggtcaa ttgaaccata catatcttga gatacatctt ctgcgcggag
    14401 agtaatagat ccaagcggaa tcgttacttc cactttagcc gttttttcac gataaacttt
    14461 taagttaatt cgggcatcca actcttgttc tggttggaag tacttttcga tcttttcgag
    14521 tttagaaact acataatcac gaattgcttc tgttacttct aggttttcac cacggatact
    14581 atatttaatc atatgagtac cttctttcta aacatttttg tttttatgat tttattataa
    14641 cgctttcatt ctatttttgc aaattttttc ctcatcttac aagggaaaat gtttttacat
    14701 ccttagcacc agcttcttcc aacagtttct taacacgatt tatagttgct cctgtagtat
    14761 agatatcatc tataagtagg atttttttag gaatagtgac tccactttta ataaagaaag
    14821 gaagttctgt ccccaagcgc tctgaacgat ttttagaaga actggctctc tcttctcttt
    14881 tctctaataa atccagatac tcaaagcctg ctgcctctac caagccctca acctgattaa
    14941 atcctctatt agcatatcta tcaggactta ggggaattac aacaaattga tactctttgt
    15001 actttttcaa ctcctcactt aaaaatgaag cgaaaacttt tcttaacagg aagtctccat
    15061 caaacttata ccgactgaaa aaatccttca tagcttgatt gtaagtaaaa atcgctctat
    15121 gactgactcc aactccttct ttacaccaaa attgacaatc ttgacacttt gttgacaact
    15181 ctgttttcat acaatttgga cagttctctt ccccaattct ttcaaaagta gaatcacagt
    15241 ctgaacaaag acaagagtca tcattcctca gaagtaagag actactaaaa gttaaaacag
    15301 tcttcatagt ctgcccacat aacaagcact tcatagacca gcctccttat tcatcatctg
    15361 aatttcctta atcgccttct tgattgaagc atttaaccca tcatggaaga aaagcaaatc
    15421 tcctgtcggt ctatccatgc ttcgtccaac tcgtccacca atctgaatca aactagactt
    15481 ggtaaacaaa cgatgattgg cttctactac gaaaacatcc acacaaggga aggtaactcc
    15541 acgctccaag attgtcgtac tgataagtat tgtcagttct ccatctcgaa aagcttgtac
    15601 ttgctctaat cgatcctctg ttacagaaga tacaaagcca attttctcat ttggaaattg
    15661 ctcctgtaag atttcttcta actgctcccc tttcttaatt tctgaagcaa aaatgagtaa
    15721 cggataagct gtctttctct gcttctcaat ataggacttt aactttggtg acaaacgatt
    15781 cttgtctaag tagcgattaa aatccgataa ccaaattggt tttggaataa tcaacggatt
    15841 tccatgaaac cgtctcgata aattcagtct ttttagttct cctaaacgga cctttttatc
    15901 taactcattg gtcgaagtcg ctgttaaaaa gattctcaat ccattctcct ttacactatt
    15961 cttgacagca tggtaaagcg tgggattatc aacataagga aaagcatcta cttcatccac
    16021 tatcagcaaa tcaaaagctt gataaaactt caataactga tgggttgttg caacaactag
    16081 tggtgttcga aaataaggtt ccgattctcc atgtagcaaa gctatcccgc aagaaaaatc
    16141 ctgttgcagg cgcttgtaca gctccaaaca aacatctatg cgaggactag ccaaacacac
    16201 tgcaccaccc gcattgatca ctttagccac tacttgataa atcatttctg tctttccagc
    16261 tcctgttacc gcatgaacta aggttggctt ttgcttgtct actgcttgaa gcagtccctc
    16321 tgacaccttc tcttgaaaag gagttaattg gccgcgccat ttgagaacat cttgcttcgg
    16381 aaaatcctcc tgcggaaaat agtataaagt ttgatcactt ctgactcgct tcatcagcaa
    16441 gcactctcga caatagtaag caccgatggg caaataccat tcttctagaa tagtactatt
    16501 acaacgttga cagaaaagtt tccccttctc ctttctcatt gctggaagtt tctccgccaa
    16561 ctgacgttct tcttctgtta attcattctc agtaaataaa cgaccgagat aatctaaatt
    16621 tactttcata cttctttatt cgtaaaaact agcactttag atgatttttt agtacaatta
    16681 aatcatggaa tttaggacaa ttaaagagga cggccaagtc caagaagaaa tcaaaaaatc
    16741 tcgctttatc tgccatgcca agcgtgttta tagcgaagaa gaggctcgtg acttcattac
    16801 tgccatcaaa aaagaacact acaaagcgac acataactgc tctgccttta ttattggaga
    16861 acgtagtgaa attaaacgta caagtgatga tggtgagcct agtggtactg ctggtgttcc
    16921 catgcttggg gtactagaaa atcacaatct caccaatgtc tgtgtggtcg tgacacgcta
    16981 ctttggtggt attaaactag gcgctggagg actaattcgt gcttacgcca gcagtgtcgc
    17041 cttagctgtc aaagaaattg gtattattga aataaaagaa caggctggca ttgctattca
    17101 aatgtcttat gctcagtacc aagagtacag taacttcctt aaagaacatg atctcatgga
    17161 gctggataca aactttacag atcaagtcga tacgatgatt tatgttgata aagaagaaaa
    17221 agaaactatt aaagctgcac ttgtggagtt ttttaatgga aaagtcactt taactgacca
    17281 aggtttacga gaggttgaag ttcctgtaaa cttagtgtaa acaatgaata atacagcgtt
    17341 tcgttgacat tctcacaact actttagcga gcaaaataaa aagatgcgta ccaaaatata
    17401 ctagaaaatg aagcaattca aacgaaacct gatatcgttt tccttcacac ctatttacta
    17461 gaattaactg aacgcaatca cttgaaaatt aatgactttg atctatgata tatagaaatc
    17521 ctatcgcttc gataggattt ctatatttta caaatggtat ggatagcgtt atactaaaga
    17581 tatcttatac aaagaggtat tcatatgtct atttataaca acattactga attaatcggt
    17641 caaacaccga ttgttaaact taacaacatc gtgccagaag gtgctgcaga cgtctatata
    17701 aagcttgaag catttaatcc tggttcatct gtaaaagacc gtattgccct tagcatgatt
    17761 gaaaaagctg aacaagatgg tattctgaaa cttggttcta ctattgttga agcaacaagt
    17821 ggaaacaccg gtattggact ttcatgggta ggtgctgcta aagggtataa agtcgtcatc
    17881 gttatgcctg aaactatgag tgtagaacga cgtaaaatta tccaagctta tggtgctgaa
    17941 ctcgtcctaa ctcctggtag cgagggaatg aaaggtgcta ttgctaaggc tcaagaaatc
    18001 gctgctgaac gtgatggttt ccttcctctt caatttgaca atccagctaa tccagaagta
    18061 cacgaaagaa caacaggagc tgagatacta gctgctttcg gtaaagatgg attagatgcc
    18121 tttgttgctg gagtaggtac tggtggaacg atttctggtg tttctcatgc actcaaatca
    18181 gaaaattcta acattcaagt ttttgcagta gaagcagatg aatctgctat tctatctggt
    18241 gaaaaacctg gtcctcacaa aattcaaggt atctcagctg gatttattcc tgatacactt
    18301 gatactaaag cctatgatgg tatcgttcgt gtaacatcag atgacgctct tgcactcgga
    18361 cgtgaaattg gtggaaaaga aggcttcctt gtagggattt cctcagctgc agctatctac
    18421 ggagccatcg aggttgccaa aaaattaggt acaggtaaaa aagtccttgc cctagcacca
    18481 gataacggtg aacgttatct ctctacagca ctttatgaat tgtaaccgtc caataacgaa
    18541 gtctattgaa aaatctccag actagagaac tcacggatag ttcctaatct ggagatttct
    18601 tatttgcact tttcttgtac aactttagtc catggtaaat aggcctctaa aacctctttg
    18661 tttacgagag tttccacgtt tggaagacat tctagaagat aggatagata tttctcacta
    18721 tttataatgg attgaaataa gatatgaaca aatcgattag gacatgatgg taaagcgtaa
    18781 tcccttgttt ctcagctttc ccagacaaaa aagtccaata gtaagtcagc tgactatcac
    18841 tctctagcac cctataagaa gtttcatccg catgaagtaa gggctgagtc aatagtctct
    18901 ctcgcaagag gttataaagg ggctccaaat agtattgact cgtcttgata tgccgattag
    18961 agatttcctt acgtgtgatt ggtaaaccca tcctagccca atcttcttct tggcgataat
    19021 tgggtacctt cagattaaac ttctgatgga tggtgtgagc gataatagaa gctgagccaa
    19081 agttatgcgc taaaggggct ttaggaatag gagatttcac aagcttatcc agatgattat
    19141 cttttactcg ttatggacaa tgctatatgg cataaatcaa gtaccttaaa gattccgact
    19201 aatattggct ttgcatttat tcttccatac acaccagaga tgaaccccat tgaacaagtg
    19261 tggaaagaga ttcgtaaacg tggatttaag aataaagcct ttcgaacttt ggaagatgtc
    19321 atacaaggac tggagaagga ggtgataaag tccatcgtta atcggagatg gactagaatg
    19381 ctttttgaaa gcagatgagt gtaaaaagaa agtcctcatt tcaatagaaa tcacaacttt
    19441 ctgatggatt tatagtaaaa tgaaataaga acaggacaaa tcgatcagga caatcaaatc
    19501 gatttctaac aatgttttag aagcagaggt gtactattct agtttcaatc tactatattt
    19561 ttggagtgat agaaaagccc ttcataagcc aatctacttg ttcaggtgcg agagctttga
    19621 catccttttc tgtacttagc caagtcagtt ttccgttctc aaagcgttta tatagtagcc
    19681 aaaatccttg accatcccag taaagggctt taaagcggtc tttacgtcca ccacaaaaga
    19741 gaaagacttg accggagaaa gaatccaatt caaagtgggt tttaactaca taggctaatg
    19801 agtctattcc ctacctcata tctgtcttgc cacaaacaag gtgaacttga cctaaatcac
    19861 ttagttgaat tatcatagta caataccttt cctccgataa ttatttttta tctagtatac
    19921 tggaagttgg ggaattagga tagatacctt gttatgacgc gcttactatg aatttgaagt
    19981 atagtctcct aaatgcactt agcccttatt atagggcttt ttgttttaat tattctaatc
    20041 gagtgagact ggggaaaaaa caatttcagg aaaaatctaa gccctataca aaaaaggaag
    20101 caatttgctt cctttctatt attagttatt caaggctgct gccattgtag ctgcaacttc
    20161 agcttcgaag tcgtttgcag ctttctcgat accttcacca acttcaaagc gagcaaactc
    20221 aactaccgaa gcgttaactg attcaaggta tgcttcaact gtcttgctgt catccatgat
    20281 gtaaacttgt gcaagaagtg tgtaagcttg gtcaacttta gtgttatcaa gcatgaagcg
    20341 atccatttta cctggaataa ttttgtccca gattttttct ggtttgcctt ctgcagccaa
    20401 ttcagctttg atgtcagctt cagcttgagc aataacatca tcagttaatt gagcttttga
    20461 tccatacttc aagtgtggaa gagctggttt attaaccatt gcacggcttt cgttgtcttg
    20521 gtcgataacg tgattcaatt gtgccaactc atctttaacg aattgctcat ccaattcttt
    20581 gtaagaaaga actgttggtt tcatcgctgc gatgtgcatt gacaattgtt tagcaagtgc
    20641 ttcgtctcca ccttcaacaa ctgaaataac accgatacgt ccaccgttat gttggtatgc
    20701 tccaaagtgt tgtgcgtctg ttttttcaat caatgcaaag cgacggaatg agattttctc
    20761 tccgatagtt gctgttgcag atacgtatgc agcttcaaga gtttcacctg aaggcattat
    20821 caaagcaaga gcttcttcat tgttagcagg ttttccttca gcaatgactt tagctgtagt
    20881 atttaccaat tcaacgaatt gagcgttttt tgcaacgaag tcagtttcag cgtttacttc
    20941 aataactgct gcaacattac cgttaacata aacaccagtc aaaccttctg cagcaacacg
    21001 gtcagctttc ttagctgcct tagccatacc tttttcacga agcaattcaa tcgctttttc
    21061 gatgtcaccg tctgtttcta caagcgcttt tttagcgtcc ataacaccgg caccagattt
    21121 ttcacgcaac tcttttacaa gtttagctgt aatttctgcc attttaattc tcctatattt
    21181 tttgaaaata ggagagccgg gctaagcccc gccctcctag gtaattacta tttgtataaa
    21241 ttaagcgttg tcaccttcta caacttcaac gatttcttca attgaatctg cttgagtttc
    21301 tgaagctgca aattctgctt caactgctac tgcatcctca ccttgacgtc cttcgataat
    21361 agcgtcagcc aatttagctg tgatcaattt aacagcacgg atagcgtcat cgttagctgg
    21421 gatgattaca tcgatatcat ctggatcagt attggtgtca accatcgcta caactgggat
    21481 tcccaatttt ttagcttctt taacagcgat ttgctcttta tgtgggtcaa ctacgtacat
    21541 cacatctggg atacgaggca tatcttcgat accgcccaag aatttttcaa gacgcgtacg
    21601 ttgtttgtta agaagtgcaa cttctttctt aggaagaact tcgaaagttc catcttcttc
    21661 catacgttta atttctttca aacgagcgat acgtttttgg attgttcccc agtttgtaag
    21721 agttccaccc aaccaacggt ggttgatgaa gtattgacct gaacgtactg cttcttcagc
    21781 aactgcatca gctgcttgtt tcttagtacc aacgaacaat acaactgcat cgttagctgc
    21841 tgcatcacgc atgaagtcgt atgcttggtc agcgtatttt acagtttgtt gcaagtcgat
    21901 aacgtggatt ccgttacgtt cagtaaagat gtacttagcc atcttaggat tccagcgacg
    21961 agtttggtga ccaaagtgta caccagcctc aagaagttgt ttcattgaaa ttactgccat
    22021 gagtatttct cctttttgtt tttttcctct tcttgacttc aactcgcaaa acgacccaag
    22081 ggcaactgct tcacaattca tcaagaatga gtattatcgt tcacacgaca attttcattc
    22141 taccatactt ttccaatatt ttcaagctat tttgatataa tttttaatag ggattgtcac
    22201 ggtaaaacct gaaaagaggg ctctattcga gtccctctgt aaattaatct gcataaatat
    22261 atgtaacaaa accttcagaa gttgttgttg gattgaacca tccacggtga tttccaattg
    22321 tacgattacc tgcataattt gattctgata cttggatacg tgttgttgat tcaacagctg
    22381 taacaaccgc tacgtgacca tatccaccat cattccaaca tgcaattgct ccaacttgag
    22441 gtgttgaacc tgtacggaaa cctgctgctg ctgcacttgt agcccactgt gctccattac
    22501 cccagtagtc tccagcccaa ggtgccaatg tttttactcc ccatgtacat tctccaattg
    22561 gataacttga agcgtttgta ctgtatgttg gacgaacttt tgcacggaca ggtgctgctg
    22621 cagattcaga tactgcttgc acttgagctg ttaagttagt gtttgctgaa gcaagtactg
    22681 attgttgttg gctagctcgt ttttctttat aagctgcttc tgctaccgca gctgcacgag
    22741 cctctgcctc agctgctgct ttttgctcta atagacttgc tttttcccct tcagctgtcg
    22801 ctttctcagc agcaagactt aattcagcag cttttagttc tgcctgtttc gtagtcaatg
    22861 cttgagcatc atcagccaat ttttgttgat tagcaattac agtattgata gcatcattat
    22921 ttgctacttg tttttcagaa atagcttttt tatctgcctt ttgttgttct aacattttgt
    22981 tgtttgcaga tacgatttca ctcattgcag caacacgtga aatagcttct gtaattgatt
    23041 ttgagtttac aatggtattg atatagctag ttacggctcc atttgtttga gcactacgag
    23101 cttgtttttc caacgattgg ttacgagaaa caatgttttt agaaagttct gtaatctcac
    23161 cctcgagttt cttagattct gcttgtaatc tatcattttc agcttgcaag ttagactgct
    23221 cagcttgaat agctgatact tgctcctgaa tttggtcaac ttgtttttgg gcttcttgtt
    23281 gttgtgctgt taagttacta attttattat cttgagcagc aattttgtca tcagtcgttt
    23341 ctgcatgcgc agttgttaaa acagctactt gagaaaccat tactgtactt aataaaagtg
    23401 acgctaagat ttttttcttc atattacgta gatactcctt cttttaaaga caatactagt
    23461 ataccaaaaa aaggcctaat aaatattac
//
LOCUS       DAVLSL010000017        22815 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLSL010000017 DAVLSL010000000
VERSION     DAVLSL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.017"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            337..2490
                     /locus_tag="VXN30_000545"
     CDS             337..2490
                     /locus_tag="VXN30_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1357213.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKEQKNGLFSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVVSEFEEKKTVEDLSLMKGEMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLDGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2503..3852
                     /locus_tag="VXN30_000546"
     CDS             2503..3852
                     /locus_tag="VXN30_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1357214.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQSEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4023..4730
                     /locus_tag="VXN30_000547"
     CDS             4023..4730
                     /locus_tag="VXN30_000547"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1357215.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4787..8512
                     /locus_tag="VXN30_000548"
     CDS             4787..8512
                     /locus_tag="VXN30_000548"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1357216.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLTEDLFVPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8605..10047
                     /locus_tag="VXN30_000549"
     CDS             8605..10047
                     /locus_tag="VXN30_000549"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1357217.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FYDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10084..11106
                     /locus_tag="VXN30_000550"
     CDS             10084..11106
                     /locus_tag="VXN30_000550"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1357218.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11103..11648
                     /gene="purN"
                     /locus_tag="VXN30_000551"
     CDS             11103..11648
                     /gene="purN"
                     /locus_tag="VXN30_000551"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1357219.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11732..12241
                     /locus_tag="VXN30_000552"
     CDS             11732..12241
                     /locus_tag="VXN30_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1357220.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12266..13813
                     /gene="purH"
                     /locus_tag="VXN30_000553"
     CDS             12266..13813
                     /gene="purH"
                     /locus_tag="VXN30_000553"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1357221.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     NDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            13935..15197
                     /gene="purD"
                     /locus_tag="VXN30_000554"
     CDS             13935..15197
                     /gene="purD"
                     /locus_tag="VXN30_000554"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1357222.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTVATVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15600..16088
                     /gene="purE"
                     /locus_tag="VXN30_000555"
     CDS             15600..16088
                     /gene="purE"
                     /locus_tag="VXN30_000555"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1357223.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16075..17166
                     /gene="purK"
                     /locus_tag="VXN30_000556"
     CDS             16075..17166
                     /gene="purK"
                     /locus_tag="VXN30_000556"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1357224.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAKVTVAPYKVVTSSQDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVQA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAQLPAIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17176..17403
                     /locus_tag="VXN30_000557"
     CDS             17176..17403
                     /locus_tag="VXN30_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1357225.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17466..18764
                     /gene="purB"
                     /locus_tag="VXN30_000558"
     CDS             17466..18764
                     /gene="purB"
                     /locus_tag="VXN30_000558"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1357226.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18819..22745)
                     /gene="strH"
                     /locus_tag="VXN30_000559"
     CDS             complement(18819..22745)
                     /gene="strH"
                     /locus_tag="VXN30_000559"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1357227.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDKAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEILSQPAPAEESK
                     ALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
BASE COUNT         6147 a         4584 c         5482 g         6602 t
ORIGIN      
        1 atttcggttt tgttaacttg taattcttat attttctagg tagaacgttt gttctgccta
       61 tttttttatc caaaaagtgc agttgggagg gagataggct catttgggaa ggaagtccag
      121 tttttgttta gtgattgggg taagatagtt gttatcagat gagtttatac tcttcgaaaa
      181 tcaaattcaa accacgtcaa cgtcgccttg ccgtatatat gtgactgact tcgtcagtcc
      241 tatctacaac ctcaaaacag tgttttgagc agcctgcggc tagtttccta gtttgctctt
      301 tgattttcat tgagtattag ggaaaaggag atgaatatga aatttgggaa acgtcactat
      361 cgtccgcaag tggatcagat ggactgcggt gtagcttcat tagccatggt ttttggctac
      421 tatggtagtt attatttttt ggctcacttg cgagaattgg ctaagacgac catggatggg
      481 acgacggctt tgggcttggt caaggtggca gaggagattg gttttgagac gcgagccatt
      541 aaggcggata tgacgctttt tgacttgccg gatttgactt ttccttttgt tgcccatgtg
      601 cttaaggaag ggaaattgct ccactactat gtggtgactg ggcaggataa ggatagcatt
      661 catattgccg acccagatcc tggggtgaaa ttgactaaac tgccacgtga gcgttttgcg
      721 gaagaatgga caggagtgac tctttttatg gcacctagtc cagactacaa gcctcataag
      781 gaacaaaaaa atggtctgtt ctcttttatc cctatattag tgaagcagcg tggcttgatt
      841 gccaatatcg ttttggcaac actcttggta accgtgatta acattgtggg ttcttattat
      901 ctgcagtcta tcattgatac ctatgtgcca gatcagatgc gttcgacact agggattatt
      961 tctattgggc tagtcatcgt ctacatcctc cagcaaatct tgtcttacgc tcaggagtat
     1021 ctcttgcttg ttttggggca acgcttgtcg attgacgtga ttttgtccta tatcaagcat
     1081 gtttttcacc tccctatgtc cttctttgcg acacgcagga caggggagat cgtgtctcgt
     1141 tttacagatg ctaacagtat catcgatgcg ctggcttcga ccatcctttc gattttccta
     1201 gatgtgtcaa cggttgtcat tatttccctt gttctatttt cacaaaatac caatctcttt
     1261 ttcatgactt tattggcgct tcctatctac acagtgatta tctttgcctt tatgaagccg
     1321 tttgaaaaga tgaatcggga taccatggaa gccaatgcgg ttctgtcttc ttctatcatt
     1381 gaggacatca acggtattga gactatcaag tccttgacca gtgaaagtca gcgttaccaa
     1441 aaaattgaca aggaatttgt ggattatctg aagaaatcct ttacctatag tcgagcagag
     1501 agtcagcaaa aggctctgaa aaaggttgcc catctcttgc ttaatgtcgg cattctctgg
     1561 atgggggctg ttctggtcat ggatggcaag atgagtttgg ggcagttgat tacctataat
     1621 accttgctgg tttactttac caatcctttg gaaaatatca tcaatctgca aaccaagctt
     1681 cagacagcgc aggttgccaa taaccgtcta aatgaagtgt atctagtagt ttctgagttt
     1741 gaggagaaga aaacagttga ggatttgagc ttgatgaagg gagagatgac cttcaagcag
     1801 gttcattaca agtatggcta tggtcgagac gtcttgtcgg atatcaattt aaccgttccc
     1861 caagggtcta aggtggcttt tgtggggatt tcagggtcag gtaagacgac tttggccaag
     1921 atgatggtta atttttacga cccaagtcaa ggggagatta gtctggatgg tgtcaatctc
     1981 aatcagattg ataaaaaagc cctgcgccag tacatcaact atctgcctca acagccctat
     2041 gtctttaacg gaacgatttt ggagaatctt cttttgggag ccaaggaggg gacgacacag
     2101 gaagatattt tacgggcggt cgaattggca gagattcgag aggatatcga gcgcatgcca
     2161 ctgaattatc agacagaatt gacttcggat ggggcaggga tttcaggtgg tcaacgtcag
     2221 agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg tcttgatttt ggatgaggcg
     2281 actagcagtt tggatatttt gacagagaag cggattgtcg ataatctcat ggctttggac
     2341 aagaccttga ttttcattgc tcaccgcttg actattgctg agcggacaga gaaggtggtt
     2401 gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg ctgatttgct tgcacaggga
     2461 ggcttttacg cccatttggt caatagctag aaagaggaga ggatgaaacc agaattttta
     2521 gaaagtgcgg agttttataa tcgtcgttac cataattttt ccagtagtgt gattgtaccc
     2581 atggcccttc tgctcgtgtt tttacttggc tttgcaactg ttgcagagaa ggagatgagt
     2641 ttgtccacta gagctactgt cgaacccagt cgtatccttg caaatatcca gtcaactagc
     2701 aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc tggttaagaa gggggatctt
     2761 ttggttcaat accaagaagg ggcagagggt gtccaagcgg agtcctatgc cagtcagttg
     2821 gacatgctaa aggatcaaaa aaagcaattg gagtatctgc aaaagagcct gcaagaaggg
     2881 gagaaccact ttccagagga ggataagttt ggctaccaag ccacctttcg cgactacatc
     2941 agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa atgagaccat cgcgtcccag
     3001 aatgcagcag ctagccaaac ccaatccgaa atcggcaacc tcatcagtca aacagaggct
     3061 aaaattcgcg attaccagac agctaagtca gctattgaaa caggtgcttc cttggccggt
     3121 cagaatctag cctactctct ttaccagtcc tacaagtctc agggcgagga aaatccccaa
     3181 actaaggttc aggcagttgc acaggttgaa gcacagattt ctcagttaga atctagtctt
     3241 gctacttacc gtgtccagta tgcaggttca ggtacccagc aagcctatgc gtcagggtta
     3301 agcagtcaat tggaatccct taaatcccaa cacttggcaa aggttggtca ggaattgacc
     3361 cttctagccc agaaaatctt ggaggcagag tcaggtaaga aggtacaggg aaatctttta
     3421 gacaagggga aaattacggc aagtgaggat ggggtgcttc atcttaatcc tgagaccagt
     3481 gattctagca tggttgcaga aggtgcccta ctagcccaac tttatccatc tttggaaaga
     3541 gaagggaaag ccaaactcac agcctatcta agttcaaaag atgtagcaag aatcaaggtc
     3601 ggtgattctg ttcgctatac tacgactcat gatgccggga atcaactttt tctagattct
     3661 actattacaa gtattgatgc gacagctact aagactgaga aagggaattt ctttaaaatc
     3721 gaggcggaga ctaatctaac ttcggagcag gctgaaaaac ttaggtacgg ggtggaaggc
     3781 cgtctacaga tgattacggg caagaaaagt tatctacgtt attatttgga tcaatttttg
     3841 aacaaagagt aatgttcgtg tttttagagt taaataattt ttaaactgtg agaaagattc
     3901 ttcttgcagt ttttttcttt acaattttcg aaaaatatct gctatttatt cggttaaatt
     3961 cttgtgtttt ttggtttttt gtggtaaaat gtgctcaagt aatacgaaag gcgaacttta
     4021 aaatgtcaaa acaattgatc tattcgggaa aagctaaaga tatctataca actgaggatg
     4081 aaaatcttat tatttcaact tacaaggacc aggcgactgc tttcaacggt gtcaagaagg
     4141 agcagattgc aggtaaggga gtcttgaata atcagatctc atcttttatt tttgagaaat
     4201 taaatgcggc tggtgtggcg actcactttg tggagaaact ttcagacacg gaacaactca
     4261 ataaaaaggt taagattatt cctttggaag tcgtgctccg caactatact gctggttcct
     4321 tttcaaaacg ttttggtgtg gatgagggaa tcgccttgga gactccgatt gtcgaatttt
     4381 actacaaaaa tgatgatttg gatgatccat ttatcaatga tgagcatgtg aaattcctac
     4441 agattgcgga tgaccagcag attgcctact tgaaggaaga aacgcgtcgt atcaatgaac
     4501 tattgaaagt ctggtttgct gagattgggc ttaaattgat tgactttaag ctagagttcg
     4561 gttttgacaa ggatggcaag attatcttgg cagacgaatt ttcaccagat aactgccgct
     4621 tgtgggacgc tgatggcaac cacatggata aggatgtttt ccgtagagga ttgggagaac
     4681 taaccgacgt ttatgagatt gtttgggaaa agttgcaggg tttaaaataa caacctcaag
     4741 gctgtttggg aatattgcaa gagctgaaat aaaggaataa gaattgatgg ataaacgtat
     4801 ttttgttgaa aaaaaggctg attttcaggt caagtcagag agtttggtta gagagctcca
     4861 gcacaacttg ggactgtcaa gcttgaaaag tattcgtatt gtgcaagtat atgatgtatt
     4921 tgacttgact gaggacttgt ttgtacctgc agagaagcac attttctctg agcaggtaac
     4981 cgaccatgtt ttagatgaag tatctgtgca ggcggatctt gctaactatg ctttctttgc
     5041 cattgaaagt ctgccagggc agtttgacca gcgtgcagct tcgtcacagg aagccttgct
     5101 tttgttggga agttcgagtg acgtgacagt caacacagcc caactttact tggtgaataa
     5161 agatattgat gcgactgagt tggaagctgt caaaaactac ctgctcaatc cagttgattc
     5221 tcgtttcaag gatatcacga cagggattgc caagcaggag ttttcagagt cagacaagac
     5281 cattccaaaa ttgactttct ttgaaagcta tacagcagaa gactttgctc gctacaaggc
     5341 cgagcaaggg atggccatgg aagtggatga tttgctcttt atccaagact actttaagtc
     5401 aatcgggcgc gtgccaactg agactgaact caaggttttg gacacttact ggtctgacca
     5461 ctgccgtcat acgacttttg agacagagtt gaaacacatc gacttttcag cttctaaatt
     5521 tcaaaagcaa ttgcagtcaa cctatgacaa gtatattgcc atgcgcgagg agttaggtcg
     5581 gtctgaaaaa ccacaaacct tgatggatat ggcgactatt ttcggtcgtt atgagcgtgc
     5641 taatggacga ttggatgata tggaagtctc tgacgaaatc aatgcctgct cagttgaaat
     5701 tgaagtggat gttgatggtg tcaaggaacc ttggctcctc atgtttaaaa atgaaaccca
     5761 caaccatcca acagaaattg agccatttgg tggagcggct acctgtatcg gtggagctat
     5821 tcgtgatccg ttgtcaggcc gttcctatgt ttaccaagcc atgcgtattt caggtgctgg
     5881 tgatattaca gcaccgattt cggaaactcg tgctgggaaa ttgccacaac aagtcatttc
     5941 taaaacagca gctcatggtt attcttcata tggtaaccag attgggcttg ccacaaccta
     6001 cgttcgtgaa tacttccacc caggctttgt agctaaacgt atggaacttg gtgccgttgt
     6061 tggtgcggct cccaagggca atgttgtccg tgaaaaacct gaagcaggtg atgtgatcat
     6121 ccttctcgga ggcaaaacag gtcgtgatgg tgtcggtggt gcgacgggct cttctaaggt
     6181 tcaaacagtt gagtctgtag agactgctgg tgctgaggtt caaaaaggaa atgccatcga
     6241 agaacgcaag attcagcgcc tcttccgtaa tggcaatgtc actcgtctga tcaagaagtc
     6301 caatgacttt ggggcaggcg gcgtctgtgt ggctatcggt gaattggcag acggtcttga
     6361 aatcgacctc aacaaggtgc ctcttaaata ccagggcttg aatggtacag aaattgccat
     6421 ctctgaatca caagaacgga tggcggtcgt ggttcgtcct gaagatgtgg atgccttcgt
     6481 tgccgaatgt aacaaagaaa atattgatgc tgttgtggtg gcgacagtaa ctgaaaaacc
     6541 aaatcttgtc atgcactgga atggtgagac aatcgttgac ttggagcgtc gtttccttga
     6601 caccaatggt gtgcgcgtgg ttgtcgatgc caaagttgtg gacaaggatg tcaaactccc
     6661 agaagagcgt caaacatctg ctgaaacact ggaatcagat acccttacgg ttctatctga
     6721 cctcaaccat gcaagtcaaa aaggattaca gactgtcttt gactgctctg ttggacgctc
     6781 aacggttaat cacccacttg gtggtcgtta ccaactcaca ccaactgagg catctgtgca
     6841 gaaattgcca gttcaacacg gtgtgactca tactgcgtcg gtcattgctc aaggtttcaa
     6901 cccatatgta gctgaatggt ctccatacca cggtgctgct tacgcggtta tcgaagcaac
     6961 tgctcgtttg gtggctgctg gtgccaactg gtctaaggct cgtttctctt accaagaata
     7021 tttcgagcgg atggacaagc aagcagagcg ttttggtcag ccagtagccg cccttctagg
     7081 ctctattgaa gctcagattc aacttggctt gccatctatc ggtggtaagg actccatgtc
     7141 tggtaccttt gaagaattga ccgttccgcc aaccttggtt gcctttgggg tgacgacggc
     7201 agatagccgt aaggtgctct ctccagaatt taaagctgtt ggggaaaata tctactacat
     7261 cccaggtcaa gccctctctg cagagattga ttttgacttg attaagaaaa attttgctca
     7321 gtttgaagca agccaagctg accataaagt gacatctgca tcagctgtca aatacggtgg
     7381 tgtagttgaa agtttggctc ttgctacctt tggaaactat attggtgcag aggtgacctt
     7441 gcctgaactt aaaacagctt tgacagctca attaggcggc tttgtcttca catctcctga
     7501 agaaattgct ggagtagaga agattggaca aacgaaagca gactttacac tgactgtcaa
     7561 cggtgtgaag ctagatggac acaagcttga cagtgcattt caagggacat tggaagaagt
     7621 ttacccaaca gaatttaccc aagcgaaaga actagaagaa gtaccagctg tggcatcaga
     7681 tgttgtgatt aaagccaaag aaaaggttga aaaacctgtg gtttacatcc cagtctttcc
     7741 aggaaccaac tcagaatatg attcagctaa ggccttcgaa aaagaaggtg cagaggtcaa
     7801 tttggtgcca ttcgtgacct tgaatgaaga ggctattgtc aagtcagttg aaactatggt
     7861 tgacaatatc gacaagacta atattctctt ctttgccggt ggattctcgg ctgcggatga
     7921 accagatggt tcagctaagt ttatcgtcaa tatcctgctt aatgaaaaag tgcgtgtggc
     7981 tattgatagc tttatcgccc gtggtggctt gattatcggt atttgtaatg gattccaagc
     8041 cttagtcaaa tcgggtctcc taccatacgg aaactttgaa gatgctaaca gtactagtcc
     8101 aaccctcttc tacaatgatg ccaaccaaca cgtggccaag atggtggaaa ctcgcattgc
     8161 caataccaac tcaccatggt tggctggtgt gcaagtgggc gatatccacg ctattcctgt
     8221 ttcgcacggt gaagggaagt ttgtcgtgac ggctgaggaa tttgcagagc tccgtgacaa
     8281 tggacaaatt ttcagccaat acgttgactt taacggtaaa ccaagtatgg attctaagta
     8341 caatccgaat ggttctgtcc atgccatcga aggaattacc agcaagaatg gtcaaatcat
     8401 cggtaagatg ggccactcag aacgttatga ggatggtctt ttccaaaata tcccaggcaa
     8461 taaagaccaa cacctgttcg catcagcggt taaacatttc actggaaaat aagacttaca
     8521 gattttctaa tagatagtat cagtaatgta aaagtcatgt aaatctagct cttgatgatt
     8581 acaaatgaaa attaggtata aaaaatgaca tacgaagtaa aatctcttaa tgaagaatgt
     8641 ggtgttttcg gtatttgggg acatccagat gctgctaagt tgacctattt tggactccac
     8701 agtcttcaac accgtggtca ggagggggca ggaatcctct ccaatgatca aggacaactg
     8761 aagcgccatc gtgacatggg gcttttatca gaagttttca gaaatccagc taatttggat
     8821 aaattgacag gagctggtgc gattgggcat gtgcgttatg cgactgctgg cgaagcttct
     8881 gtagataaca tccagccctt cctcttccgt ttttacgata tgcagtttgg cttggctcat
     8941 aatggaaatc tgaccaatgc agcctctctc aagaaagaac tggaacaaag aggagcaatt
     9001 ttcagcgcga cttcggactc tgaaatcttg gctcacctca ttcgtcgcag tcataatcct
     9061 agcctgatgg gcaaaatcaa ggaagcgctc agccttgtca aaggtggttt tgcctatatc
     9121 ttgctgtttg aggacaagtt gattgcggct cttgacccaa atggattccg accgctttcg
     9181 attggtaaaa tggctaatgg agcagtagtc gtttcctctg aaacctgtgc ttttgaggtc
     9241 attggtgccg agtggattcg tgatttgaag ccaggtgaga ttgtgatcat tgatgacgag
     9301 ggcattcagt atgatagcta tacagatgat acccagttgg cgatttgttc tatggagtat
     9361 atctactttg ctcgccctga ttctaatatc cacggtgtca atgtccatac ggcacgtaag
     9421 agaatgggag cacaattggc gcgagaattt aagcatgagg cagatattgt agttggtgtg
     9481 cccaattctt ccctaagcgc ggctatggga tttgcggaag aatcaggctt accaaatgaa
     9541 atgggtctga tcaaaaacca atacacccag cgaactttta tccaaccgac tcaagaattg
     9601 cgggagcaag gggtgcggat gaaactgtct gctgtttcgg gtgttgtcaa aggcaaacgt
     9661 gtggtcatgg tggatgattc cattgtacgt ggaacaacct ctcgtcgtat cgttcagctc
     9721 ttgaaagaag cgggtgcgac tgaggttcac gttgccattg gaagtcctgc actagcgtat
     9781 ccatgtttct acgggattga tatccagacc cgtcaggagc tgattgcagc caatcatacg
     9841 gtcgaagaaa ctcgccaaat cattggtgcg gacagtctga cttatctttc aattgatggc
     9901 ttgattgagt cgattggtat cgaaacagat gcgccgaacg gtggtctctg tgtcgcttac
     9961 tttgacggtg actacccaac gcctctttat gactacgaag aagactatcg tagaagtttg
    10021 gaagaaaaga ccagttttta caagtaggcg acagattctc cattaaagaa aaggaaaaaa
    10081 caaatgacaa ataaaaatgc atatgcccag tcgggtgtgg atgttgaagc gggttatgaa
    10141 gttgttgaac ggattaaaaa gcacgtggcc cgtacggagc gtgcaggtgt catgggagct
    10201 cttggtggct ttggtggtat gtttgacctt tccaagactg gggttaaaga acccgtcttg
    10261 atttcaggga ctgacggtgt cggaaccaag ctcatgttgg ctatcaagta cgacaagcac
    10321 gataccatcg ggcaggactg tgtggccatg tgtgtcaacg acatcattgc tgcaggtgcg
    10381 gaacccctct attttctcga ctacgtagcg acagggaaga atgaaccagc taagctagaa
    10441 caagtggttg ctggtgtggc agaaggttgt gtgcaggctg gtgctgccct catcggtggg
    10501 gaaacggctg aaatgccggg catgtacggc gaagacgact atgacttggc tggttttgcg
    10561 gtcggtgtgg ctgaaaaatc tcaaatcatt gacggttcaa aggtggtaga gggagatgtt
    10621 cttctcggac ttgcttcaag tgggattcac tcaaatggtt actctttggt tcgtcgtgtc
    10681 tttgcggatt acacaggtga ggaagtccta ccagaattgg aaggcaagaa acttaaggaa
    10741 gttctacttg agccgactcg tatctatgtc aaggctgtct tgccgctcat caaagaagag
    10801 ttggtcaacg gcattgccca catcacaggt ggtggcttta tcgaaaatgt ccctcgtatg
    10861 tttgcagatg acctagctgc tgaaattgat gaaagcaaag ttccagtgct tccaattttc
    10921 aaagcccttg aaaaatatgg tcagattaaa cacgaagaaa tgtttgaaat cttcaatatg
    10981 ggtgtgggac ttatgttggc ggtcagccct gaaaatgtag agcgtgtaaa agaattgttg
    11041 gatgaagcag tctatgaaat tggtcgcatc gtcaagaaag aaaacgaaag tgtcattatc
    11101 aaatgaaaaa aatagcggtt tttgcctctg gtaatggctc aaattttcag gtgattgccg
    11161 aagaatttcc agtggagttt gtcttttcag accatcgtga tgcctatgtg cttgagcgtg
    11221 caaagcagct cggcgttctg tcctatgctt ttgaactcaa ggagtttgag agcaagacag
    11281 actacgaagc agcccttgtc gaactcttgg aagaacacca gattgacttg gtttgcctag
    11341 caggctacat gaaaatcgtt ggaccaacct tattgtcggc ttatgaaggt cggattgtca
    11401 acattcatcc agcctacttg ccagaatttc caggagctca tgggattgag gatgcttgga
    11461 atgctggcgt gggtcagtct ggtgtgacca ttcactgggt ggattcgggt gtggatacag
    11521 gccaggtcat caaacaggtt cgtgtgccac gactagctga tgataccatt gacagatttg
    11581 aagctcgcat ccatgaagca gagtacaggt tgtatccgga agtagtgaag gctctattta
    11641 cagattgact ttttgatgat tcatatgata tctttgattt taaattggag tcagtgtttg
    11701 ttgaagacgg cttcaaacgg aggtatttgt aatgttagaa tctaaaaaaa caactcgata
    11761 tgtattttat gtctatctga tgttattaac ttggggaatc ttatttaagt ttgaaacaaa
    11821 tcctgaattt atagcatttt tcttagctcc aaggtatatc aattggattc cattttcaga
    11881 accactaata gtcgatggaa aaattgtttt tgctgaaatg ttatttaatc tgatttcctt
    11941 tattccatta ggtgtttgtt tccctttgat aaaaactaat ttatctagtt taagaatagt
    12001 cgggacaggt ttcttgatta gtttattgtt tgagtgctta cagtatattt tagcaatagg
    12061 tataacagat ataacggatt tgactttaaa tacgctaggt gtctgtgtag gcttactgat
    12121 ttatcaaatt tttataagag tgttcaaatc acagactaga aaatggatca atatcttagg
    12181 tatgcttagc cttggttttg cttatcttgt tttactgtta ctgcatttaa ttggtgttta
    12241 actaatgatt aaaaaggaga atataatgac taaacgcgtc ttaatcagcg tctcagacaa
    12301 agcgggcatt gttgaatttg cccaagaact caaaaaactt ggttgggaga ttatctcaac
    12361 aggtggaact aaggttgccc ttgataatgc tggggtggat accattgcta tcgatgatgt
    12421 gactggtttc ccagagatga tggatggccg tgttaagact cttcatccaa atatccacgg
    12481 gggtcttctc gctcgtcgtg atttggatag ccacttggaa gcagccaatg agaaccaaat
    12541 tgagcttatt gaccttgtag tggtcaacct ttacccattt aaggaaacta tccttaaacc
    12601 agatgtgact tatgctgatg cagttgaaaa tatcgatatt ggtgggccgt ctatgcttcg
    12661 ttcagcagcg aaaaatcatg ccagtgttac agttgtggta gatcctgctg actacgctgt
    12721 ggttttggac gaattggcag caaacggcga aacctcttat gaaactcgcc aacgtttagc
    12781 agccaaagta tttcgtcaca cagcagctta tgacgccttg attgcagaat acttcacagc
    12841 tcaagtgggt gaaagcaagc ctgaaaaact cactttgact tatgacctca agcaaccaat
    12901 gcgttacggt gagaatcctc aacaagacgc ggacttttac cagaaagctt tgcctacaga
    12961 ttactctatt gcttcagcca aacagctcaa cgggaaagaa ttgtcattta ataatatccg
    13021 tgatgcagat gctgctatcc gtatcatccg tgacttcaaa gatagtccaa ccgttgtggc
    13081 tctcaaacac atgaatccat gtggaattgg tcaagctgat gacatcgaga ctgcttggga
    13141 ctacgcttat gagtctgacc cagtatctat ctttggtggg attgtcgtcc tcaaccgtga
    13201 ggtggatgct gcgacagctg agaagatgca cggcgttttc ctcgaaatca tcattgcacc
    13261 aagctatacg gatgaagcgc tagctatttt gatcaataaa aagaaaaact tgcgtatcct
    13321 tgccttgcca tttaatgctc aagaggctag cgaagtggaa gcagaataca caggtgtagt
    13381 cggtggactt ctcgtgcaaa atcaagacgt ggtcaaggaa agcccagctg actggcaagt
    13441 ggtgaccaaa cgtcagccaa ctgagacaga agcgactgct cttgagttcg cttggaaggc
    13501 tatcaagtac gtcaaatcaa atggtattat cgtgaccaac gaccacatga cacttggtgt
    13561 tggtccaggt caaaccaacc gtgtggcttc tgttcgcctt gccattgacc aagccaatga
    13621 tcgtctggac ggggcggtcc ttgcttcaga tgccttcttc ccatttgcgg ataacgtgga
    13681 agaaatcgcc aaagcaggaa ttaaggccat catccagccc ggtggctctg tccgtgacca
    13741 agaatccatc gaagcagcga ataaatacgg cttgactatg gtctttacag gtgtgagaca
    13801 ttttagacat taagaagata aaagggaaga aaacagtttc tttccttttt tggcttaaaa
    13861 tgctaactga aacaagatta aaacgaactt ttttgatata atgttggtaa ataattcgca
    13921 aaagaggttg aggaatgaaa ctgcttgttg tcggttctgg tggtcgtgag catgcgattg
    13981 ctaaaaagtt acttgaatca aaagacgtgg aaaaagtctt tgtagctcct gggaatgatg
    14041 ggatgactct ggatggtttg gaattggtaa atatctctat ttccgaacat tctaaattga
    14101 ttgacttcgc aaagaccaat gatgttgctt ggacctttat cggtccagat gatgcccttg
    14161 ctgctggtat cgtggatgat tttaaccaag ctgggcttaa ggcctttggt ccgactaggg
    14221 ctgcagcgga gctggagtgg tccaaggatt ttgccaagga aatcatggtc aaatatggtg
    14281 tttcgacagc agcctatggc acattttcag atttcgagga agcaaaggcc tatatcgaaa
    14341 agcatggtgc acctatcgta gtcaaggcgg atggcttggc gcttgggaag ggtgtcgtcg
    14401 ttgctgagac ggttgagcaa gcggtcgaag ccgctcatga gatgcttttg gacaataaat
    14461 ttggtgactc aggtgcgcgc gtggttattg aggaattcct tgaaggagag gaattttcac
    14521 tctttgcctt tgtcaatggt gataagttct acatcatgcc aacggctcag gaccacaaac
    14581 gtgcctacga tggcgacaaa gggcctaaca cgggtggtat gggtgcctat gcgccagttc
    14641 cacacttacc acagagtgta gttgatacag cggttgacac cattgtcaag ccagttctag
    14701 aagggatgat taaagaaggt cgcccttatc tgggagttct ttacgcaggg cttatcctga
    14761 cagctgatgg accgaaagtc attgagttca acgctcggtt cggagatcca gaaactcaga
    14821 ttatattgcc tcgtttgacc tctgactttg ctcaaaatat cacagatatc ctggatagca
    14881 aggagccaaa tatcatgtgg acggacaagg gtgtgactct gggtgtggtt gtcgcatcca
    14941 agggctaccc gctagactat gaaaggggcg ttgagttgcc agccaagaca gaaggcgatg
    15001 tcattaccta ctatgcaggg gctaagtttg cggaaaatag cagagcactg ctctcaaacg
    15061 gcggacgagt ttatatgctg gttaccacag tagccaccgt caaagaagcc caagccagca
    15121 tctaccaaga actatcccaa caaaaaatag aaggactctt ctaccgaaca gatatcggaa
    15181 gcaaggcaat taagtaaaga tataagaata acgcgccgta gtcaccaaac acgataatgg
    15241 tcgtcgtggt gaaaagacca gaacagtgaa tgttctggtc agggggaaac ttggagacct
    15301 taggctcaaa gtttaggaat gaaaccgaag gtttgcttcc gcctccatca cctaagacca
    15361 ttatcaaaaa gaaaaataaa aattcacaaa atacgttaat gatcgtatgg tttgcgagcg
    15421 ttagcgagct aatatagaac aatcaccgcc gttgtgaaag aacgattgga tgataatcca
    15481 atcgttcagg gaaattggaa gaccttgggt ttccaattta ggcatgagac acctttggtg
    15541 gctgctgccg tccctcacaa gctaaggtga ttgttgaaaa agaggaaaaa ggagaagaaa
    15601 tgaaaccagt aatttccatc atcatgggct caaaatccga ctgggcaacc atgcaaaaaa
    15661 cagcagaagt cctagaccgc ttcggtgtag cctacgaaaa gaaagttgtt tccgcacacc
    15721 gtacaccaga cctcatgttc aaacatgcag aagaagcccg tagtcgtggc atcaagatca
    15781 tcatcgcagg tgctggtggc gcagcgcatt tgccaggcat ggtagctgcc aaaacaactc
    15841 ttccagtcat tggtgtgcca gtcaagtctc gtgctcttag tggagtggat tcactctatt
    15901 ctatcgttca gatgccgggt ggggtgcctg ttgcgaccat ggctatcggt gaagctggag
    15961 cgactaacgc agctctcttt gccctccgtc tcctctctgt agaagataag tccattgcgg
    16021 atgcacttgc caactttgct gaagaacaag gaaaaatcgc agaggagtcg tcaaatgagc
    16081 tcatctaaaa caatcggaat tatcggtggc ggtcaactgg gtcagatgat ggccatttct
    16141 gctatctaca tgggccacaa ggttatcgcg ctggatcctg cggcggattg cccggcctct
    16201 cgtgtggcgg aaatcattgt ggcaccttat aacgatgtgg acgccctccg tcagttggca
    16261 gaccgttgcg atgtcctcac ttacgagttt gaaaatgtcg acgctgacgg tttggatgcc
    16321 gttatcaagg atggacaact ccctcaaggg acagatctgc tccgcatttc gcaaaatcgt
    16381 atttttgaaa aggacttttt gtcaaacaag gccaaagtca ccgttgcacc ttacaaggtt
    16441 gtgacctcaa gccaagattt ggcagatatc gacttgtcga aaaactatgt cctcaagact
    16501 gcgactggtg gctacgatgg tcatggacaa aaggttattc gttcagaagc agacttggaa
    16561 gcagcctatg cgctagcaga ctcagcagac tgcgtcttgg aagaatttgt caactttgac
    16621 cttgagattt ctgtcatcgt gtcaggaaat ggcaaggagg tgacgttttt cccagttcag
    16681 gaaaatatcc accgcaacaa tatcctgtct aagaccatcg tacaagcccg catttctgaa
    16741 agtctagcag acaaggctaa agctatggca gtgcgaatcg cagaacaact caacttgtct
    16801 ggaactctct gtgtggaaat gtttgcgact gatgatgacg ttattgtcaa tgaaatcgcc
    16861 ccacgaccac ataactctgg ccactactcg atcgaagctt gcgacttctc gcagtttgat
    16921 acccatattc tgggtgttct gggagcgcag ttgccagcta tcaaactcca tgcgccagct
    16981 gtcatgctca atgtcctagg tcaacacgtc gaggctgctg aaaagtatgt cgcagaaaat
    17041 ccaagcgccc acctccacat gtatggtaaa atagaagcaa agcataatcg taagatggga
    17101 catgtgacct tgtttagtga tgtaccggat agtgtggatg agcttgggga agggattgat
    17161 ttttaggaca agtctatgat acaaattatc gttaatacat ttattgaaaa ggataagact
    17221 ggagcagttg ttgaagtgtt gtatgccagt gctgaccaag ataaggtaca agctaaatat
    17281 gaagaactag ctgcacaata ccccgaaaat tatttagcta tctatgatgt accgctggat
    17341 acggatttga atacactaga tcattacccg tctgtgttta ttggaaaaga ggagtttgag
    17401 tagaaatctt ggtttaccta gatagcttat tcccaacagc ttaagaagaa aggaaaaatt
    17461 aacacatgat caaccgttac tctcgccctg agatggcgaa tatttggagt gaagaaaata
    17521 aataccgtgc ttggcttgag gtggaaatcc tctctgacga ggcatgggct gagttggggg
    17581 aaatccctaa ggaagatgtg gctttgattc gcaagaaggc ggactttgac atcgaccgta
    17641 ttttggaaat tgagcaggag acgcgccacg atgtggtggc tttcacgcgt gcggtttctg
    17701 agactcttgg tgaagagcgc aagtgggttc actatgggtt aacttctacc gacgtggtgg
    17761 atactgctta tggttacctc tacaagcagg ccaacgacat catccgtcgt gaccttgaaa
    17821 acttcactaa tatcatcgct gacaaggcca aggagcacaa gttcaccatc atgatggggc
    17881 gtactcatgg tgtgcacgct gagccgacaa cctttggtct taaattggca acttggtaca
    17941 gcgaaatgaa acgcaatatc gagcgcttcg agcatgcggc tgctggtgta gaagctggta
    18001 agatttctgg tgcggttggg aactttgcca atatcccacc atttgtagag gagtatgtct
    18061 gcgataaact tggcatccgt gcccaagaaa tctctacaca agtccttcct cgtgaccttc
    18121 acgctgagta ctttgcggtt cttgccagca ttgcgacttc aatcgaacgt atggcgactg
    18181 agattcgtgg tctacaaaaa tctgagcaac gcgaagtaga agagttcttt gctaaagggc
    18241 aaaaagggtc ttcagcaatg cctcacaaac gcaacccaat cggttctgaa aatatgactg
    18301 gtctggcgcg tgtcattcgt ggtcacatga ttacggctta tgaaaacgtc gctctctggc
    18361 acgaacgcga tatttctcac tcatcagctg agcgtatcat cacaccagat acgaccattt
    18421 tgattgacta catgctcaac cgttttggaa atatcgtcaa gaacttgaca gtcttcccag
    18481 aaaatatgat ccgaaacatg aactcgactt ttggtcttat ctttagccaa cgggctatgt
    18541 tgacattgat tgaaaaaggc atgacccgtg agcaagccta tgacttggtg caaccaaaaa
    18601 cagcctactc ttgggacaac caagtagact ttaaaccact tcttgaggca gattcagaag
    18661 taacatcacg tctcacacaa gaagaaatcg atgaaatctt caacccagtt tattacacca
    18721 aacgagtgga tgatatcttt gaacgtcttg gactaggtga ttaattaaaa aataaacagc
    18781 gagcttcaat ctcgctgttt attttttatc gaaaagactt agtcttcttt tcttttagtg
    18841 agtccatagg ctgctagtgt ggccatgagt cctgcgacta ctagtcctgc agaatcgtga
    18901 gttcctgttt caggaagttt tttctctatt accacaggag ctggatcttg aggaagagct
    18961 ttgctttcct cagcaggagc tggttggctt aggatttcta gttttggttt ttcttcagca
    19021 atagcggctt gtccgttttc atcgcctaca tgtgttacca tagttccgac ttcgactatt
    19081 tgagtaacgg cttcctgtgc tacgacacta tttacaagtg ttttcacttc cttaccatcg
    19141 gcagaagtgc tcacagagta gaagttgcta cgatgtccat tgacgccctt agtaatgact
    19201 tgtgtttttc ctttgagtaa gagtggattt tcacgagtca ctgtggtaaa tggaatttct
    19261 tcttcttgga tatccagtct aggttttacc tcagtagttg gtgcaagacc actttcatca
    19321 cccttgtgag ttacaggagc gccaacttca accacttggt ttataacttc tttggttacc
    19381 tggctatcaa ggactgtttc tgttgttttt ccattttcag tgagtacaga gatgtaatga
    19441 gttcgttcac ctttgactcc tgctgtgata atattttcct gaccagctgg gaggttagga
    19501 ttttctttct tgataacttc aaatggaatt tcttcagttc ttgtgatgag ttctggtctg
    19561 gtttcaacat tggcagccac ttcattttca tctaggcttc ctgaatgagt tgcagctggt
    19621 ttgaggcctt gaagagcggc ttttaggttg gctacaagtg tgtcaagctc agcttgttta
    19681 ttacggttga ggttgtaatt tagagctgtt ttagctgcgt caagggcctc aagactttct
    19741 ttactatatc cttctaagtt tgtaggaatt ttagctaatt cttcgcggag agcattataa
    19801 ttagcacgga agtagtcttt gttgtggtct gcaaaggcag tcatgagttc aaagatttcc
    19861 tcttccttgt attcagcgct tggtctatct gcccagattg aaagcatact tccgactgtt
    19921 ggaagatcta cttcaggata tttggtagaa gctagttgat tgaatggaac tttaccagaa
    19981 ttttcaactg cttttgaaag agggtaagct tcatcttgtt tgtgattgcc tataacataa
    20041 taccagtcac cgttggtatt caagaactta taacccttgc ttgctaggta ttgaggtgat
    20101 gcgaggttgt atccccacca gcctttagac cagtaagaaa tcaagacatc tttgtcaaac
    20161 tgaacatcgt ccttgtcttc atagtagaag ccatcgttga aggccattgg ttgaagccct
    20221 ctttctttgg ccatagctgc gagggtgttg gcatattcgg caaatttgcc atagagttga
    20281 taccacttga ggtagtacca gccttgggca ctagtcgcat cgttggcgta ttcgtcagta
    20341 ccaaagttga aaatctttgt tttacctgca aagaagtcca tgtatttacc gatgagggct
    20401 tttacaaagt tcatcgcttc ttcgtttttc aagtccatag ttgtttttga aactttatca
    20461 aagtgggctt gaggattttt aatacctaat ttttccatgg caaccagcat agcatccatg
    20521 tgacctggac tgttaatagc tgggatgaga ccaatgtcct tagatttagc gtattcaatt
    20581 agctctgtca cttctgcctg tgttagtgca gtaccgtttg gatcgtcgta gtaagcttta
    20641 gttccttcga taatagcttt tttaacgtca tcactagcat aggtttttcc gttggcagta
    20701 atggtcatat cattgagtag aaagcgaagt ccgtcatttc ctagaaggag atggacatca
    20761 gaatatccga gctcactggc cttgtctacg atgcgtttga gctggtccag agtaaagtat
    20821 ttgcgtccag catcgattga gattaccttg tttttggcaa gtttttcaac ctcacgttta
    20881 gcttcttctt ctttttgagc ttcaggcgtg agggtcaagt tgttgacagt ttcttgaagt
    20941 ttagcaatgg ctttatcaat cgtatcttgt tgggcacggc taaggttgct atcgagagag
    21001 cgaatagctt tttcagcttc ttttacggcc gtgacgcttt ctgcagtata acggttcagg
    21061 tcttttggta cctcgttaag tgcttgctct gcagattcat aatcagctgc gaagtattca
    21121 gcgttggcat ttgcaaaatg acgcatgagt ttgaagaggc gtgatggtga ataacgtgca
    21181 gatggagtgt cagcccaagc agctaccata ccaccgatga ttgggatatc agctccttct
    21241 gtttttggta cagaagtgat tggtgtgttt ttaataccat tgagcccctg atcgagattg
    21301 taccagcctt ggccatcagc gtttcgtcca agaacgtagt accaagcatc attggtatta
    21361 aggatttggt gacctttttc agctagtagt ttagaagaag cgacatcgta gcctccccaa
    21421 ccaccagtcc acatagaaac gatgatgtct ttgtcaaaac taccaaagct tgtgtcgcta
    21481 ttgtagtaga taccgtcgtt aaaggccatt ggtttgagac cgtgcgattt tacaatacga
    21541 gcgaggtcat tggcgtaggc aataaatttt tcatagcctt ttacagggta gccttcgttt
    21601 ggatagtatt tatcagcttg aagcacactc caacctttag catctgtcgc atcattggca
    21661 tattcatcaa gtccgatgtt gaagatttca gtctttttcg cgaaataagc agcatacttg
    21721 tcgataaggg cttttgtaaa agcgacagct tgttcgttgt caagatcgac agtacgggct
    21781 gatttcttcc caaaatagct aaagttaggg ttttggattc ccaattcttt catggcattg
    21841 agaatcgcat ccatgtgtcc aggactattt actgtcggaa tgagaccgat acctttatct
    21901 ttggcatagt taatcagatc tgtcatttga ctttctgtta agtgattgcc gtttggatcg
    21961 ttgtaataat catttgtacc tttttcaatg gcgcgtttga catcgtcact ggcataggtc
    22021 ttgccgttag ctgtgatgct catatcgtcc aacatgaaac ggagtccatc atttccgact
    22081 aataggtgta aatcagtgta gccataatgt ttcgctttat cgatgatttc cttgagctgt
    22141 tctggtgaga aatatttacg tccagcatca atagaaacaa ttttcttttt cgctagtttt
    22201 tcatttacag ttgcagcacg ttcctttcct gtctctgttt ccggtttgtc agcctctgct
    22261 ttcgcttcat cttttttagc tggtttatcc ttgtcagtct tgtctgtatt tgactcttta
    22321 gaatcaacct ctttcgcttc ttccttttta gggctagctt cttcttcctt tttattagca
    22381 gtttcttttt cagcagaagt tggagttacc acctctgctt tatcactagg agttgaacta
    22441 acttcctctt gtggtttttc ttctgttttt ggaagactag ctaccttatc agtagctgga
    22501 gtttctgttt ctacagtttt tggagcttct ggttgaagct ctgctttagg tgtttcctca
    22561 gtccgatttt cggatgattg aggggaatca gaaaccgtat ggatggtcgg ttggttttct
    22621 gtagtagtag gagtaactcc atcggctgca acagtctgtg cttggaaggc aaatccaatt
    22681 agaacagaag ctgctcctac agcgtattta cgaatagaaa aacgctgttg tttttcatgt
    22741 ttcattgcaa aacctcctga ttgcattgtt atattgatag cgattatata aatcaacgcc
    22801 tttattttat ttctt
//
LOCUS       DAVLSL010000018        22398 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLSL010000018 DAVLSL010000000
VERSION     DAVLSL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.018"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            378..995
                     /locus_tag="VXN30_000560"
     CDS             378..995
                     /locus_tag="VXN30_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1357228.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(1315..2202)
                     /locus_tag="VXN30_000561"
     CDS             complement(1315..2202)
                     /locus_tag="VXN30_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1357229.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVVRHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(2351..4234)
                     /locus_tag="VXN30_000562"
     CDS             complement(2351..4234)
                     /locus_tag="VXN30_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1357230.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     SQGNKVKAGDVLGKFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            4611..6065
                     /locus_tag="VXN30_000563"
     CDS             4611..6065
                     /locus_tag="VXN30_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1357231.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGIYYELDYGHKTN"
     gene            6046..7011
                     /locus_tag="VXN30_000564"
     CDS             6046..7011
                     /locus_tag="VXN30_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1357232.1"
                     /translation="MVTKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(7046..8320)
                     /locus_tag="VXN30_000565"
     CDS             complement(7046..8320)
                     /locus_tag="VXN30_000565"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1357233.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQIANPKLAQEKIASKKAELLELANQAYPSIVKRGGGASDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(8320..9492)
                     /locus_tag="VXN30_000566"
     CDS             complement(8320..9492)
                     /locus_tag="VXN30_000566"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1357234.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(9623..9871)
                     /locus_tag="VXN30_000567"
     CDS             complement(9623..9871)
                     /locus_tag="VXN30_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357235.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(9939..10562)
                     /locus_tag="VXN30_000568"
                     /pseudo
     CDS             complement(9939..10562)
                     /locus_tag="VXN30_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10624..11073)
                     /locus_tag="VXN30_000569"
     CDS             complement(10624..11073)
                     /locus_tag="VXN30_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1357236.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(11075..11833)
                     /locus_tag="VXN30_000570"
     CDS             complement(11075..11833)
                     /locus_tag="VXN30_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1357237.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(11948..13927)
                     /gene="pknB"
                     /locus_tag="VXN30_000571"
     CDS             complement(11948..13927)
                     /gene="pknB"
                     /locus_tag="VXN30_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1357238.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(13924..14664)
                     /locus_tag="VXN30_000572"
     CDS             complement(13924..14664)
                     /locus_tag="VXN30_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1357239.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(14679..15992)
                     /gene="rsmB"
                     /locus_tag="VXN30_000573"
     CDS             complement(14679..15992)
                     /gene="rsmB"
                     /locus_tag="VXN30_000573"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1357240.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(15985..16920)
                     /locus_tag="VXN30_000574"
     CDS             complement(15985..16920)
                     /locus_tag="VXN30_000574"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1357241.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(16933..19329)
                     /locus_tag="VXN30_000575"
     CDS             complement(16933..19329)
                     /locus_tag="VXN30_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1357242.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(19395..19709)
                     /locus_tag="VXN30_000576"
     CDS             complement(19395..19709)
                     /locus_tag="VXN30_000576"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1357243.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(19734..20360)
                     /gene="gmk"
                     /locus_tag="VXN30_000577"
     CDS             complement(19734..20360)
                     /gene="gmk"
                     /locus_tag="VXN30_000577"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1357244.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(20490..22103)
                     /locus_tag="VXN30_000578"
     CDS             complement(20490..22103)
                     /locus_tag="VXN30_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1357245.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT         6174 a         5199 c         4446 g         6579 t
ORIGIN      
        1 tgattaatat taaaattaac taacaattca aaggattcat actagccata aattacgtcc
       61 atcagagaga gactcttact acttttagat tttagtcttt ctagcttcag aatacatcta
      121 aactttaggg aaaatgacta ttcgaaagcg cgaatgcctc aaaattatct cagataagct
      181 attcgaaact tagaatgctt ttaaatttat ggaattgcga ttattcgaaa cctagaatgc
      241 atataacctt tagttgacag acctattcta agtctcgaag ggctatttac tttctattcc
      301 ttatcaaaaa agactcattc cccctttctc ctccaaaata tggtatagta gaaatatact
      361 atctatgagg agtttacatg tcacaggata aacaaatgaa agctgtttct ccccttctgc
      421 agcgagttat caatatctca tcgattgtcg gtggggttgg gagtttgatt ttctgtattt
      481 gggcttatca ggctgggatt ttacaatcca aggaaaccct ctctgccttt atccagcagg
      541 caggcatctg gggtccacct ctctttatct ttttacagat tttacagact gtcgtcccta
      601 tcattccagg ggccttgacc tcggtggctg gggtctttat ctacgggcac atcatcggga
      661 ctatctacaa ctatatcggc atcgtgattg gctgtgccat tatcttttat ctagtgcgcc
      721 tatacggagc tgcctttgtc cagtctgtcg tcagcaagcg cacctacgac aagtacatcg
      781 gctggctaga taagggcaat cgttttgacc gcttctttat ttttatgatg atttggccca
      841 ttagcccagc tgactttctc tgtatgctgg ctgccctgac caagatgagt ttcaagcgct
      901 acatgaccat catcattctg accaaaccct ttaccctcgt ggtttatacc tacggtctga
      961 cctatattat tgactttttc tggcaaatgc tttgacacgt aaaaaatccg tttggtttcc
     1021 caagtggatt tttaaagcgt agattaacta tagcttgata ctaaatatac tttggtatgg
     1081 aaatcatgca tatttttcga tagtgaggcg aggacttacc tagccttccc gccgtgatag
     1141 aaacacctga aatctaatgg tttcaggtat tcggaaactt tgagcctagt gtctcaaagt
     1201 ttaggtatgg aatttcgaag aaagtcgcta ccgtccgtaa tcacttaagg aaaggctcaa
     1261 aaatattgtt ttcaaccaca aaatccgttt ggtttcccaa gcggattttg tgctttattt
     1321 tgaaacttct tttgcaagaa caaagttccc aagtgtggca gaaccatttc ctgcgactgc
     1381 tggcgtcacg atatagtcac gcacatctgg tactggtagg taaccattaa gaagagatgt
     1441 aaatttctca cggacacggt ccagcatatg ttgttgagcc atgacccctc caccaaagac
     1501 aatcacgtct gggcggaaag tcactgtcgc attaaccgca gcttgagcga tatagtaggc
     1561 ttgaacatcc caaacagggt tgttgagttc aatattttcc ccacgtacac ctgtacgagc
     1621 ttccaaactt ggaccagctg catagccttc cagacatccc ttatggaaag gacaaacacc
     1681 cttaaactct ttttcaatat ccattgggtg tctaacaaca taataatgac ccatttcagg
     1741 gtgacccaca ccaccgataa actcaccacg ttggatgaca cctgcaccga tacctgtacc
     1801 gattgtgtag taaaccaagt tttcgatacg accaccagca ttgttacggg caaccacttc
     1861 accgtaagca gagctgttta cgtctgttgt gaagtacatt ggcacgttta gggcgcgacg
     1921 aagggcacca agcaagtcca catttgccca gtttggtttt ggagtcgtcg tgataaagcc
     1981 ataagttttt gagtttttgt caatatcaat cggcccaaat gaaccaactg caagaccagc
     2041 aaggttatcg aattttgaga agaactcaat ggttttatcg attgtttcga ttggagttgt
     2101 tgttggaaat tgtgtttttt ctacaacgtt aaagttttca tcaccgacag cacagacaaa
     2161 ctttgtaccg cccgcttcca agcttccata taattttgtc atgataaacc tcttgttttt
     2221 attttcttta ttatagcata cttcgaaagt ctaaatgtct ctatttttta gattttcctc
     2281 tgtaaatctt actatctaat aaaaacgaac aaacatgtca tttgttcgtt ttcacattag
     2341 agaggattga ttagattttc acttcgatca cagcatcccc cttcgaaact gaacctgttg
     2401 cgactggagc tactgaagcg tagtcacctg tatttgtaac gataaccatt gttgtatcat
     2461 caagtccagc tgcagcgatt ttgtttgagt caaattttcc cagaacatcg ccagctttca
     2521 ccttattacc ttgagaaact tttgcttcaa aaccgtcacc gttcatagat acagtgtcaa
     2581 taccaacgtg aatcaaaact tcagcaccat ttcttgtttt caaaccaaaa gcgtgccctg
     2641 ttggaaaggc aattgaaact tcagcatcag ctggtgcata gaccacgcct tggcttggtt
     2701 tcacagcgat accttgtccc atagctccac ttgagaagac tgggtcattg acatcagcaa
     2761 gagcgacaac atcaccgacg ataggagtta caagtgtttc attttgaaga gctgctggcg
     2821 caacttcttc tttttcttca gccacttcag cttgttttgc agctgcagtt gcgtctactt
     2881 catcttcgta accaaacatg taagtaagag caaaaccaag ggcaaatgat acagctacca
     2941 taagaaggta ttgtggaagt tgtccgttac ccacataaag cattgtacca gggatgatgg
     3001 tgataccatt accagtacca gcaagtccaa ggatagaagc caatccacca ccgattgcac
     3061 cagcaatcaa tgaaaggaag aatggtttac ggaagcgcaa gttcaccccg aagatagcag
     3121 gctctgtaat acctaggaag gcagaaagag cagccgggaa agcaagtgtt ttcagttttg
     3181 gattttttgt tttaacacca accgcaacag tagcagcacc ttgagctgtc atagcagctg
     3241 tgatgatagc gttgaatggg ttagcatggt cagcagcaag taattgcact tcaagcaagt
     3301 tgaagatgtg gtgcacacct gacacgacga tcaattggtg aaccccacca atcaagaaac
     3361 caccaagacc aaatggcatg ctaagaatcg cttttgtagc aataaggatg tagttttcaa
     3421 caacgtggaa aactggtcca atgacaaaga gtccaaggat agacatgacc aaaagtgtca
     3481 cgaatggtgt taccaagagg tcaatgacat ctggaacaac cttgcggaca gctttttcaa
     3541 atttagctcc gacaaccccg atgatgaagg ctggaagaac agaaccttgc aaaccaacaa
     3601 cagggatgaa accaaagaag ttcatcgctg ttacttcacc accttgagca actgcccaag
     3661 cgtttggaag tgagccagag acaagcatca taccaagaac gataccaacg gcaggatttc
     3721 caccaaatac acggaaggtt gaccacacaa ccaaacctgg caagatgatg aaggctgtat
     3781 ctgtcaagat ttgtgtgtaa gttgcaaagt cacctggaag tggcatttca agagcgttga
     3841 aaagaccacg cacacccatg aagagacctg tcgctacgat aactgggatg attggaacga
     3901 aaacatcacc gaaagtacgg atagcacgtt ggaaccagtt cccttgttta gcaacttctg
     3961 ctttcatgtc atccttagat gatgttggta agccaagtac aacaacttca tcgtacattt
     4021 tgttaactgt acctgtacca aagataattt ggtattgccc tgagttaaag aaagcacctt
     4081 gaactttttc caagttctca atcacttctt tattgatttt ctcttcatct ttgaccatga
     4141 cacgtagacg agtcgcacag tgggcaacac tattgacatt ttcacgtccg cccaaggcat
     4201 cgatgacttt ttttgcaatt tcctgattgt tcatttgcaa aaatctcctt atataacatt
     4261 ttgttcttgt ttgaaagcga ttttattcgc ccttacgact attattttat catgttttaa
     4321 aaatatgtca agcgttttgc agaaaaaata ttctatctac ttagtgggca agctttagat
     4381 tgattgtttt tgactgtttc atcaggaatt tcttgaaaaa taagtttgaa accttggtta
     4441 aaaagtcttt tatttcatta tctttcacat tttcgtaaaa atttgattga tttcctgtat
     4501 ttattttatg ataatcgttt gacagttttt tctctttttt aatataaaag gcttgcattt
     4561 tttatcgaaa acagttacta ttaaaagtga taagattttt ggaggaatga atggaatgga
     4621 caactgagcg tcgttacaga ctttatcaag attggacgca agaagaaatt caacatataa
     4681 aggaaaatat ggcacaatct ccatggcata ctcattacca tgttgagcca aaaacaggac
     4741 ttctcaacga cccaaatggc ttttcttact ttgatggcaa gtggatcctc ttttaccaga
     4801 attttccttt tggtgcagcc cacggtttga aatcttgggc acagctagaa agtgatgatt
     4861 tggttcactt taaagaaact ggaatcaaag ttttaccaga tactccatta gatagccacg
     4921 gtgcctactc tggttctgcc atgcaatttg gcgataactt attcctattt tatacaggaa
     4981 atgttcgcga tgaaaactgg atccgtcacc cataccagat cggtgctttg atggacaagg
     5041 agggtaagat tacaaagatt gacaagatct tgattgacca gccagcagac tctactgacc
     5101 acttccgcga tccacaaatt tttaacttta aaggtcaata ctatgctgtc gtcggtggac
     5161 aggacttgga gaaaaaaggc ttcgtccgtc tctacaaggc tgtcaataac gactacacaa
     5221 actggcaagc agttggcgac cttgactttg ctaacgaccg tactgcctac atgatggaat
     5281 gtcctaatct ggtctttgta gaggaacatc ctgtccttct ctactgtcca caaggattgg
     5341 ataagaaagt tctagactac gataatatct ttccaaatat gtataagatt ggggcttcct
     5401 ttgaccctaa aaatgccaaa atggtagatg tgtctcaact tcaaaacatg gattacggtt
     5461 tcgaagccta tgcaactcaa gccttcaacg ctcctgatgg gcgtgctcta gcagttagct
     5521 ggcttggttt gccagatgtt tcttacccat ctgaccgttt tgaccaccaa ggaaccttct
     5581 ctttggtcaa ggaactcact atcaaagacg acaagctcta ccagtatcca gtcgctgcta
     5641 ttaaggacct tcgtgcttct gaagaagcct tctcaaaccg ttcccaaacc aagaacactt
     5701 acgaacttga actcaacttg gaagctaata gccagagcga gattgtctta cttgctgata
     5761 aagaaggtaa gggactttca atcaactttg accttgtaaa cggtcaagtg acagtggatc
     5821 gtagccaggc tggagaacag tatgcccaag aatttgggac aactcgttct tgccctatcg
     5881 agaatcaggc tactactgct acaatcttca tcgataactc tgtctttgaa attttcatca
     5941 ataaaggaga aaaagtattt tctggtcgtg tcttcccaca tgcggaccaa aatggtatcc
     6001 tgatcaaatc tggaaaccca actggaattt actatgaatt agattatggt cacaaaacta
     6061 actgatgtcg ccaaacttgc aggcgtcagt cctactaccg tttctcgggt tatcaataaa
     6121 aaagggtatc tatctgagaa aaccatccaa aaagtcaatg aagccatgag agaattgggc
     6181 tataaaccca acaacctggc tcgtagtctg caaggaaaat cagctaagtt aatcggcttg
     6241 attttcccca atatttccaa tgttttctat gcagaattga ttgataaatt ggaacaccaa
     6301 ctcttcaaaa atggttacaa gaccatcatc tgcaacagtg aacatgattc tgagaaggaa
     6361 cgcgaataca tcgaaatgtt ggaagccaat caggtagacg gcatcatttc tggtagtcac
     6421 aacctaggaa tcgaagacta caatcgtgtg acagcgccga ttatttcctt tgaccgaaac
     6481 ctatcgccag acatccctgt tgtctcctct gacaactatg ctggtggggt tcttgctgcc
     6541 caaaccttgg tcaagacagg tgcccagtct atcatcatga ttacagggaa tgacaattct
     6601 aattcgccaa ccggactgcg ccacgctggt tttgcatccg tactcccaaa agctcctatt
     6661 atcaatgttt ccagtgactt ttctcccgtc agaaaagaaa tggaaatcaa gaatatcttg
     6721 acccgggaaa aaccagatgc catttttgct tcggatgatt tgacagctat tctggtcatt
     6781 aaaatcgctc aagaattggg catttctgtc ccaaaagagc tcaaggtcat cggctatgat
     6841 gggacctact ttatcgaaaa ttactaccct caattggcta ctatcaagca acctttggaa
     6901 gagattgctt gtctcactat tgatcttctc ttgcaaaaga ttgaaggcaa ggaagtcgcc
     6961 acaactggtt acttcttacc agttacacta ttaccaggaa aaagtattta aacacaagaa
     7021 aactcagacc gattcgtctg agtttttatg atcttaaatt ttcgagatag cgctgggctg
     7081 tctctaggtt aaaggtttta tctgagatga ggcgctctac taggggagca acttcagatt
     7141 cactagcccc agctaggaga gctagggatt tggcctgtag tttcatgtga ccttgctgga
     7201 tgcccgtact taccaaggct ttgagggctg caaaattttg agcaagaccg atggacacga
     7261 taatctgggc taattctctg gcagaaggat ttcctagtag atcatgactg agagctacac
     7321 gtgggttgag gccgatagag ccacccttag tcgctacagg catgggcagg gtcatctcac
     7381 cgaccaattc ttctctttca aggtccagcg tccagcagct aagaccttga tagcgtccat
     7441 ctcgactggc aaaggcatgg gccccagctt cgatggcacg ccagtcatta ccagtggcaa
     7501 tcaaaatcgc atcaatacca ttaaaaattc ctttattatg agtagcagct cggtaaggat
     7561 cagcctgcgc aaactgacta gccaatgcaa ttttctccgc aatctctcgt ccttgatcct
     7621 tttggcggct caagtagcga aaggccatgc gacagcttgc agtcaccaga gaatcggtcg
     7681 cgtagttgga caggattccc atgagactct gtccctgact gagttcttct aagactggtt
     7741 tcaaggcttc cagcatggtg ttgagcatat tggcacccat ggcttcctgg gtatcaacat
     7801 gaatataaac aacgagaaag tctggttcac cttttatctg ctcgacatgc aggtcactcg
     7861 ccccaccccc acgtttaacg atagaaggat aggcttgatt ggcaagctcc aagagctccg
     7921 ctttcttgct ggcaatcttc tcttgcgcta gtttaggatt agcaatttga taaagggcta
     7981 cctgcccaat catctggcgc tgatggactt gtgcagtaaa accacctgca cgcttgatga
     8041 ttttgctggc atagctggcc gccgcaacca cagagggttc ttctgtcaca tagggaacgg
     8101 tgtattcctg accgttgaca agtacctccg gaaccagcga ataaggcaga gaaaaagttc
     8161 ccaccacatt ctcactcagc tggtctgcca cagtcacact catctgttca tccttctcca
     8221 gactagcttg tctctcagga ctaaggagcg cctgagcttt tagcagctcg aggcgctctt
     8281 ggtatgattt tttagaaaat ccattccaac ttatcttcat tatttttcaa ccttgctata
     8341 acggcgttgg tggtcaagaa tttcaaccaa ggcaaaatct tgattttcat agccagcaaa
     8401 ctgggcagag tttgtttcat ccaagtttac ttcctcaaaa aagacctttt catagtctgc
     8461 aacggatagg gcagttcgtt ggttgagctt gttcaagcgg tctttatcca aataagcttc
     8521 atatccttca accaattcac cactgaagaa ctcagccaca gctccacttc cgtaactata
     8581 aagggcgatt ttatccccag ctttcaagct atctgtattt tccaagagag acaaaagtcc
     8641 aaggaaaagt gaacctgtgt agatattccc caccttttga ctgtagagaa tagactggtc
     8701 aaaatgcttt tgtaagaggt cttttttctc ttgaggcagg ctcttatcca tgattttttt
     8761 caagcctttt agcgctaatt taggataagg caagtggaaa caaacagccg caaaatcatc
     8821 cagagtaagc tggtagcgtt tttgatattc aagccaagtc gttttcaaac tatccaagta
     8881 ttgttgggta gaataaacac catttacata aggagttgtc gagtaatttg gtcgccagaa
     8941 atccatgatg tcacgggtct gagctacatt gtcattatta aaggccatca tgcgtggatt
     9001 ttgtgtaatc aacatagcta cacttccagc accttgagtt ggttctcctg gagtttcaat
     9061 accgtatttg gcaatatcac tggcaatgac caagaccttg gactccggag aattttccac
     9121 atgcaatttg gcataatgaa gggcagcagt cgctccataa caggcttctt taatctcgaa
     9181 actacgagca aagggctgga tgcccagcaa gccatgcaca aagacggccg cagccttact
     9241 ctggtcaatt cctgactcgg tagccacaat gaccatgtca acttcttgtc tttcttgctc
     9301 agttaaaata gagtcactag cactggccgc caaggtcaca atgtcctcag ttaggggcgc
     9361 aatactcaat tccttgagta agagtccttt acttaatttt tcagggtcaa ttcccctcgc
     9421 ttctgctaag tcttgtaatt tcaagacata ttgactggtc gcaaaaccaa tcttatcaat
     9481 accgattgtc atatttacct ctgttttatc attcatgtaa aaaatcgttc tatactattt
     9541 tatcacaaat ggcagtaaaa gagagaaaaa agacttgatt caccaaatca agcctcttat
     9601 tggtcatcat tttaaagaat gattagttgc tagagagttc accgatataa gtagctttat
     9661 aagctccatt cacagttatc agctcctgga ggatcaaatt tcctgagtaa gtccttccca
     9721 tctcatctac aaatttttga taaaactgac tggtcggaat ttctctgaca tccttatcaa
     9781 atgtcttatc aagtgtttta ctaaccttct cagcaatcaa ttgatgctct tgccatccac
     9841 tttgaaactc tgagcccgaa ctagaaacca tgactgggat aaacaacaag gtcagtagat
     9901 ttacagacaa taaggaaagt agtagacttc ctgcaaaact agaatcctag ttcatgattg
     9961 ataataccag caatcaaatt cattcgtaat ccgaagcgtt tacgatgatt tcgataggtt
    10021 gttgaaaaca ttttaaacgt ttttactttg gcaaagatgt tctcaacctt gcttctctcc
    10081 ttagatagcg catggttaca ggctttatct tcagctgtta gcggcttgag tttgctggat
    10141 ttacgtggag tttgtgcttg aggatatatc ttcatgagcc cttgataatc actgtcagcc
    10201 aagattttac cagcttgtcc gatatttctg caactcattt tgaacaactt catatcatga
    10261 ctatagttca cagcgatatc caaagaaaca attctccctt gacttgtgac aatcgcttga
    10321 gccttcatag cgtgaaattt ctttttacca gaataattcg ctaattgttt tttagggcga
    10381 ttgattttta cttccgtcac atcaatcatt atcgtgtcct caaagctgag aggagttctt
    10441 gaaatcgtaa caccactttg aacaagagtt acttcaaccc attggctccg acggattaag
    10501 ttgctttcgt gaataccaaa atcagccgca atttcttcat aagtgcggta ttctcgcaca
    10561 tatagaaagc gttatcaatt tatttatctc atttttcaga aaattctttt atttctgtaa
    10621 agtctacgat actcgatgtg tttttatata atgatagagt ctgagaatca ctgttccgct
    10681 agccattcca atagagatta ccaaagccaa catgacaacc aaggtcgcac ttgccagtgc
    10741 tttattatag tcccctgtca caaaaaaggc agttgttcgg taggagagat aacctggaac
    10801 cagcggtgcc aaaatggcca agataaagac cacagcaggt gtcttataaa gaatacttaa
    10861 aatctggctg acacaagaac caataatggc tgcaatgaag gtagctacaa tgacattggt
    10921 cggttccttg agcaagagat agattagcca gacagtcatg cccaaaatcc ctccaggtaa
    10981 gagcatagac cgttgcacat tgagtacgat taaaaaagtg ataatggcaa gaaaacttgc
    11041 tactgcttgt aataaaaagg ttgttagtgt catattagtt catcaatacc aaggcgacag
    11101 aagttcctgc ccctaaagcg agggtaatga gcagggattc aaacatctta ctcataccag
    11161 agtttatgtg gttggtcata atatcacgga ccgcattggt caaggcaata cctggtacaa
    11221 acggcatgac cgcaccagct ataatcaaat ctgccgttga aggaaaacct gtgtagcgag
    11281 cccaaaactg ggcaattatc ccaaagacaa aggctccagc aaaggctgtc acaaagggaa
    11341 ttcggataaa tttttccaca tagagggaaa aggcaaaacc aaataaggtc gccactcctg
    11401 ccccaagtgc gtcgtagata tttccgctaa acataactga aaagaaagga gcactaaagg
    11461 tcgcagccag agttacctgc aacttagtat agggaagggg tttggcttgc aaggccgtca
    11521 attgcttaaa ggctgtttct aagtcaatct gccccccaac tagctgacga gaaatctggt
    11581 tcacatcgca gactttttcg atgttataag aagaggaggt cacgcgcttc atgcgcgaaa
    11641 tattggtatt ttcaatagag aaaaagatag cggcaggcat ggcaaggaca ttgcaatcca
    11701 caatcccctg cgaatgcgcg attcgaatca tggtatcttc tacacgatgg atttctgagc
    11761 cacttttaag gagaatagtc cccgctagca taatcacatc aatgacggca tttaattctt
    11821 ttgattcttc catgctttcc tccttttatc aactccctct attctatcac aaatccggac
    11881 tcaaaaaaaa tctttgccat gaaatcatga caaagattga ttactcattt tgattatcca
    11941 tctgctttta aggagtagct gaagttgttt taggtttgta gattgaaatc ttgactctag
    12001 tcttatttag gtctaccttt tcacctgctc taggactttg ttcaacaacc atgccttcta
    12061 cactacctgc aggcgctgtc gtcacttcta caacttctat attagcttcc ttaatcccaa
    12121 caatttgaat caaattgttc ttagtaaact ccaagctgga accaatgtaa ctcggcatgg
    12181 caacacttgt aactttttta gctactgtca agacaatttg agtaggttta ctcacatcat
    12241 aagtcgttcc ggcacctgga ctttgtttca taatcgttcc tggttcgctt tcgctggact
    12301 cttcttcctc tatcttaatc aaattctcag gaaccttctt ctgcttgagt tctgagatta
    12361 cttctgtaga gttccgtcca atatagttcc ctaattgaat cgtcgtagct tttttagcta
    12421 ctgtcaaaac aatttgagtt gccttgctca agtcataggt cgtaccttct ggtagacttt
    12481 gcttcaggac cgttccagcc tcactctcat tggactcttc ttcctcaatt ttaatcaaat
    12541 tatctggaac ttttttctct tttaattccg cgataacatc agaagatttc cggccgacat
    12601 aattactaat ttggaaggat tgtttgcctg atgagacaac cagattgatt ttcgttcctt
    12661 cttttcgacc agttccagcg ccaggatctg tacggataat ccgcccttct tccacctttt
    12721 cactagcctc tgtcttctcc tcaccaatct caaaattggc ttttttgagc gttgccttgg
    12781 cctctgcaac tgtctgacct gccacatccg gaatggcaat ggttgcagga gttctggata
    12841 agatccaaat aagagaagct gccaccaata caaggctggc caacaaaatc aggtaacgca
    12901 tcttaaatct atgttttttc ggtgcttgtg gttggtaagt ttcctctgtc acagcctggc
    12961 ttgggttttt gattgatttg tgttctgtct gcgcttgaac cttaggaata gatgtcaagg
    13021 tactctgaga aaccttcggc aaggtcttgg tatctgcctt gctcgtttca tcaaagatta
    13081 acttactttc atttctacga ttgtaggaca agctactaga caagtccaca tacatctctg
    13141 aaaccgatcg gtagcgattg gtcaactttt tagcagttgc cttgataata acattttcta
    13201 aagcctgagg tacagatgga ttttctgcaa taacggacgg caggggtttc tggaaatgct
    13261 ggagggcaat ggtcaccgcg ctatccccgt cataagggat atggcctgtc aacatctcat
    13321 agaaaataat ccccatggca tagatatcac tctgcacagt cgccttagaa ccacgcgcct
    13381 gctctggtga caagtaatga actgagccca acatcgagtt agtctgggtc agacttgtct
    13441 ctgcaaaggc tacagcaatc ccaaagtctg tgaccttggc agtcccatct ggtgtcaaaa
    13501 ggatattttg aggtttcaag tccctgtgaa caattcctcg agtatgggcc aagcgcatag
    13561 ccaagagaat ttgtcccatg atacggacgg cttcttcatt agaaagagga taatgttcct
    13621 tgatatagcg tttgaggtct agtccagcaa catactccat tgcaagatac tgttgaccgt
    13681 cttcctcacc aatatctgtt atccgaacga tatgaggatg gtctagatct gccatagctc
    13741 tcgcttcacg ctgaaaacga gctacagcta tcgggtccgt ctggtagttg gtcctcagaa
    13801 ccttcactgc cacttcttcc ccatctaaga ttaagtcttt ggctaggtaa acatccgcca
    13861 tgcctcctcg accaatctgt ttgacaatcc gatagcgtcc ggcaaaaatc ttgccgattt
    13921 ggatcattct tcatcctcct cgttcataga aacaagggca accgtaatgt tgtctaaacc
    13981 tcctgcattg ttagcaaaac gaacaagtgt ctccgtttta tctgctaaag gaatatcact
    14041 ggttacaata tcacgaatct cactgcctga aatcatgttg gtcaagccgt cactattgag
    14101 caagagatag tcacctgact caaggataac tgtcccaaaa tcaggctgaa tttcatcttt
    14161 ttgcccaata gactgggtga taatattttt ttgcggatga gcttctgcct cttctggtgt
    14221 caattgacca gccttgagca attcattaac caaggaatga tcgctcgtca actgatggta
    14281 ttcttctcca cgaatcaagc cgatacgcga atcaccaata tgagcataga tagcctgatt
    14341 atcaataata gcaaggactt ccaaagtagt tcccatgcct ctgtaagctt catcctgacc
    14401 aagctggtga atcttttgat tttcaatttc taggtaatgg gcgaaccatt cacgcacttc
    14461 attgactgta tcgatctggg tatcaaccca agctacaccc aggtctgtga ccgccatttc
    14521 actagcgata ttccctgcac gatgacctcc catcccatca gctaaaataa tcatggtacg
    14581 tccagctcta ttgacatagt ggttgacata gtcttggtta tttgttcgtt tctgaccaac
    14641 atctgttaat aatgaaattt ccatgtgtca gttccttcct aatccgatat cttgcgaaat
    14701 tgactgatga agaatccatc acttccatac aattcaggtg taatgaggat acagccgtct
    14761 ttcatgatat ccttacattc atgttctagt tttacctgct cgaactcggg atgactctct
    14821 aaaaaggcct taacgacttg aaaattctcc tctgagacga tagtgcaggt gctataagtt
    14881 attataccac ctttgcctag tatttgacaa acactaccta atatttctaa ctgaatttcc
    14941 tgcaaggacg cgaaatctgc cgtttcttta ttgtatttga tatctggttt tcggcgcaaa
    15001 agaccgattc ctgaacaagg agcatccacc aaaatcttat caaaggaatc ctggtcaaaa
    15061 aactcatgca cctttctggc atccaatttt tgagtttgaa cccgatcttc aactcccaga
    15121 cgttgggcat tttcttgaat taaatccaac ttgtggtcgt acaagtccag agcagtaacc
    15181 tgacctgtcg taagataaga ggctatatgg gctgttttcc cacctggagc cgcacaggca
    15241 tcaagcactc gctcatcacc ttgtaaatca agcgtcggag caaccagctg actggactcg
    15301 tcttggatgg taatggctcc atccgcaaac aaattatgcc ctgcaaaatg cccctgctcc
    15361 ttaaccagac cagtggttgc taaaagggaa tcattcgcct ccaacaaggc ttggatttcc
    15421 tcttttcgac ttaggtctgt tacacgaata ctggctttgt ttcgcactaa caggctttca
    15481 aagatggctt ttgctctctc ctctccgtat tcttccttga gtttggcaac tagccaaact
    15541 gggagagaat aggcaatgga gtcacgcttg ttttttcgct tgatgctagc aatatctggc
    15601 cagccttcac gcaagatacg gcgaaggagg gcattgacca atttttcact gcctttttta
    15661 cgaagtttgg ccaattccac tgcttcattg accacagcat gatctggaat cttgtccaaa
    15721 tagcggagtt ggtaggcact catgagaaga aggacataga gccagctgtc taactggtct
    15781 ctgtcttcga taaagtggga taggtaccat tccagagtca gtttacgggc taccgttcca
    15841 tagaccagct cggtcactaa gcccttgtct gctgccaaaa gttgactccc ctttagatgc
    15901 ttatttaagg cgatatttga gtatgcttgg ttcacaaaaa catcctctag cactgctaga
    15961 gctaaacttc tagccgtttc tactttagtc accaaatcgt tctcctacag tcaatgtacg
    16021 tccaactccg ttgaggaagg aagcaatgtc catcttaggc ttaccagctg gctgcacttg
    16081 tttgagggat agagcccctt cagccgttgc gacaatcaat tctttcttgc cgatagagag
    16141 aatctcacct ggatttccct gaccttctac tggtagggct tcataaatct taaagcggtc
    16201 gcccttaagg aaagcatggg caacaggcca ggggttcatt ccacgaattt ggttaaagag
    16261 ttgacgattg gttttgttcc agtccagttt ttcttcctct ggctttatat ttggagagaa
    16321 ggtaacctga ctcgtatcct gcggttcagg tttgatatca ccagcaatat aggcaggcag
    16381 agtgtccaaa agcaaatcac gaccaactag cgccaatttt tcaaacaagg tgccaacatt
    16441 gtcctcatct gtgatcggaa tgctgcgacg agaaatcata tctcctgcat ccatttcctt
    16501 aaccatttcc atgatggtca caccagcttc ctcatcccct tgaatcaagg cataatggat
    16561 aggcgcacca ccacggtgtc taggaaggag ggaggcatga acgttgacag caaagtccat
    16621 gctatcaagg agtttgcttg ggagaaactg cccaaaagca gcagtcacaa ttccatctgc
    16681 tcctagcttc ataagttctt ccatctctgg acttccagat aatttttcag gttggtagat
    16741 agatagtcct gcttccttgg cagcctgctt gactggggtt tcttggataa cttttttacg
    16801 accaacagca cggtctggct gggtcacaac ggctagaatt tcgtaacggt catctgtcaa
    16861 aagtcctttt aagactgttg ctgaaaagtc gggggtcccc ataaagatta gttttgtcat
    16921 atcttctcct tcttataaaa attgctgcgg ctcatggtca atgctgagac ggagctcact
    16981 attttcccgt tcttgagtca aggccaagac ctggttgagg gtcggcccca gctcatcttc
    17041 taaacggtat ttaattaaaa tctggtaatg atagaggttg tgggtacggg caatcggttt
    17101 tggcgttggc cccagaatgg gactggtctc tgacaagcct gaccgcaaaa tgttcatgac
    17161 ttcataggca cgtttgaaaa cctcttcttc tttcttgtga gaaagggtaa taccaatcgt
    17221 gaaatagtaa ggtggatagc cgagttgtcg tctgattccc atttcatagg cataaaagcc
    17281 ttcgtaatct tgatccttgg caaatcgaat agcatagtgc tgcgaattgt aggactgtat
    17341 caagacttga cctgcttttt cagcccgacc tgctcgacct gccacctgag tcaagagctg
    17401 gaaggttctc tcagaagaac ggaaatcagg cagattcaag gccgtatccg catttagaac
    17461 tccgactagg gtaacattgg gaaaatccaa accctttgca atcatctgag taccaagtaa
    17521 aatatccgct tcccctcgcc caaactggtc aagcaaggct tggtgactgc ctttctttcg
    17581 agtcgtatcc acatccatcc tcaaaatgcg agcttgggga aagagttctg ctagctcatc
    17641 ataagccttc tgagttcccg tcccatagta acgaatactg cggctcttac agttaggaca
    17701 gacctgagga atatccttcg agaaaccaca ataatggcag ttcatagtct tggtatccat
    17761 atgcaaggtc agagaaatat cgcagttggg acaagtatcc accgtcccac actcccgaca
    17821 catgacaaag ctagaataac cacggcgatt gagcatgaga accacctgct cttttttaac
    17881 cagacggtct tggatagcct ctagcaaagg aggcgtaaag tttgacgtct cattttgtcc
    17941 gatatagtct cgaaagtcaa tcacttgaac ctcagggatt gtagccaaag gattggcacg
    18001 ttgggttaga cgtaagtgtt gatagacgcc tttgccagca cgtgcccggc tctctaagct
    18061 cggcgttgca gatccaagta ccagagttgc ttgattatac tgagcccgta aaatagctac
    18121 ctctctggca tggtaacggg gattgctgtc ctgcttataa gccgcttcat gctcttcatc
    18181 aataatcatg acacccagat ttttcagagg agcaaagata gcagatctgg caccaacaac
    18241 aacttgggca tcgccacgct ccaccttgcg ccattcatca tacttttcac cattggataa
    18301 tcctgagtga agaatggcta ccttgtcccc aaaacgtgct ataaaacgct cggtcatctg
    18361 aggagtcaag gaaatctcag gtaccagcaa aatagctgtc ttgcccttat ccagggcacc
    18421 ttggataatc tgcaagtaaa cctcggtctt cccacttcct gtaatccctt gaagtagaaa
    18481 gggaggttga gaactgccaa tagaactcac aaccgcatca cgcgcctgtc tttgttctgg
    18541 atttaactcc aaaggtctac ttgcttcaat tccttcaaaa taagcagccg agcgttgaac
    18601 ttccttttgg actatggtaa cagcaccttg atccacaaag aagttgactt gctctcgcga
    18661 gtaggactct aacaagctag ccaaggaagc gctctctgga tgagacagca gataatctct
    18721 cagttccaac tttttcttgg cacgtgtaga aatctcaaca ccttctaatt gggcatggtc
    18781 aacctcatac caagactggg tcttgacctt cttttgatcg actgcctgat attccagacc
    18841 aagcaggcct tttctagtca aacgcatcat ttcagcttgc ttggcaaggt ctagtgaaga
    18901 aaaggctagc gaatcttctg aaccaaacag gcgcactcgt tcttcctgac tcaagccttc
    18961 cagaggatag agaatcttgt catagctaga attcagaaac cctggaagca tggccttgag
    19021 gatagagatt ttgtaggaga agacagattt gcgtaactcc tcagccagcc agagttgttc
    19081 tggcgtgaga acaggagaaa aatccagcac ctctgcaata tcttttaaat cttgctccat
    19141 ctcttctcca tctgattggg acttcaaacc aagaacaatc ccttgaatca ggcgattacc
    19201 cttaccaaaa ggcacatgaa cccgcatccc aacttccagc attccctcaa attcctccgg
    19261 aatcctgtaa ctatagggct ggtccgtctg catcaagggc acatctacga taatcttagc
    19321 tagggccatc ttctcacctc ctccttgtca gcacattctt gcaatagaaa aaataagatt
    19381 gagtcccccc aaccttaaat tttttcacca tcttcttttt ctttagcaat ttgctctttg
    19441 attttctttt cttcttcttc tttgcggcgt ttttcttctt cgatacggcg acgcactgct
    19501 tcacgttttc cttctggatc tgggtgaatt gtaacgtttc ctgattcgat ttcttctaaa
    19561 gcgcgaagag ttgatttttc agacttgaaa ccttgagttg ctggggcacc tgcttccaat
    19621 tcgtgggcac gttttgcttc caagattacg agtgaatatt ttgaaggaac cttgtcgagc
    19681 aaggtatcaa tagagggttt taacatcatt tgcttgtacc tattttctaa attttatcgg
    19741 gtagttggag attttggtaa catctcctga tagtgaccaa tgacacgatc cacacagaag
    19801 tgttctgctt caatcacaca tttgacacgt tcagcagcta ggggcacctg atcgttgaca
    19861 atcgcataat catactcacg catgagggca atttcttcct tggccttttc gattcgttgg
    19921 gcaatcactt ctgcactatc tgttccacga cctaccaagc gatcttgcaa ttcatccaaa
    19981 tctggtggtg tcaggaagat aaagacagca tctggaacct ttttcttgac ctgaagagca
    20041 ccctgaactt caatttcaag gaaaacatcg attcccttgt ccaaggtttc attgacatag
    20101 gtcagaggag ttccatagta gttaccgaca tattctgcgt attccaacat ctgtccttga
    20161 cgaatcagct cttcaaattc ttcacgagta cggaagaaat agtcaacacc gtccacttct
    20221 ccaggacgtt gtgcgcgtgt cgtcatcgat acagaatatt gaaattggtt ttcagaactc
    20281 tcaaaaatct ctcttctaac cgttcctttt ccaacccctg aaggaccaga aaaaacgatt
    20341 agtaagcctc ggtctgccat tgtgtctcct tttagtcaat ctgtgaaata acatttctct
    20401 agaataatgg caaaaagcca gattatcctt tacagtcttt ctatctagtg taacaaaaaa
    20461 gcagtaattt ttcaactgct ctttcttatt tatttagcat aatctactgc acgaagctcg
    20521 cgaatcacgg ttaccttgat atttcctgga taatcgagat tgttttcaat tttcttacga
    20581 actttgtgag ccaagattgt gactttgtcg tccttgattt ttcctggatt gaccatgata
    20641 cgaatttcac gtcctgcttg gagggcaaag ctagtttgca ctccttcaaa gccgttagca
    20701 atttcttcca aatcatggag acgcttgatg tagctttcaa gagactcact acgagcacct
    20761 ggacgggctg cgctcaaggc atctgctgca gcgacgataa ctgctatcac gctctcagct
    20821 tcaacatctc cgtggtgact agcaatcgta ttcaccacaa ctgggggttc cttgtactta
    20881 cgggccaatt ccataccgat ttcaacgtgg ctaccttcaa cctcatggtc aatggctttc
    20941 ccgatatcgt gaaggaatcc agcacgacgg gcaagagccg cattttcacc aagttcgctc
    21001 gccatgatac cagccaactt agcaacctca atcgaatggc gcaaaacatt ttgtccatat
    21061 gaagtacgga actgcaaacg tcccataatc ttcatcaagt ctggatgaag gtttggcgca
    21121 ccaatttcat aggcagcagc ctcaccgtat tcacgaatct tattgtcaat ctcttgacgg
    21181 tttttctcaa ccaactcttc gatacgagct ggatgtatac gaccatcttt gagcaacatt
    21241 tccatagtca tacgggcaat ctcacgacga atcggatcaa atcctgacaa ggtcaccact
    21301 tctggtgtat cgtcgataat cacatcgacc cctgtcaaac tttcaaaggt acgaatgtta
    21361 cgaccttcac gaccaataat gcgtcccttc atagtatcgt ctggcagatg aactgttgag
    21421 tttgttgact ccgctacata ttcaccagcg atacgttgca tagcttgaac caagatgtcc
    21481 ttggccattt tgtcagaacg ttccttgacc tcttgctcag cttcgcgaat gcgactggca
    21541 atctccctgg tcaagttttc ctctgtctga gccaagataa tatctcgtgc ttctgcctga
    21601 gacagagcac caatacgctc tagttctgct tctttttgtc tttcgacttc ctctaattgc
    21661 tcttcacgcg catcaaggtt tttcgctcta tcagaaatac tttgttcttt ttgttcaagt
    21721 gtttgttctt tactcgtcaa attgtcgtcc ttacggtcaa ggctagtagc tctctctgtc
    21781 aaacgacttt cgatttgttt gagttcttga cgttctgatt tgaattcagc gtccacttct
    21841 tcacggtatt ttctggcttc ttctttggcc tccaatagtg cttctttttt aagagacttg
    21901 ctttcacgtt tggcttcatt aacaagtaaa tccgcttcac gctcagcttg tccacgtaaa
    21961 ttagttgctt cttgttcagc atttaaaagc atcaactctg cagcttcctg agatgatttc
    22021 atcttagctg agatgctgac atatccaatg actaaaccaa tgatgacggc aaaaacagca
    22081 atcgcaagcg acatgatttc catgttttta cctcatttta ttgttattcc gaatgacata
    22141 cattctttta cattctacca taaaaaagtg attttcacaa acctaaaata gaatatgttt
    22201 tgaggaaatt agtctgaatt ttaggattac acttccttgg ttttttaagg agataagtca
    22261 gtgccgctat caaaaagttc caccgaattt ctaccgctat ggattttcta ttgttgtgcc
    22321 aaatagtgtt ccactagatt caaacatttt gctttcaagt ctttgaaaat tcactttagg
    22381 aaatcacagc ttatcttc
//
LOCUS       DAVLSL010000019        21907 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLSL010000019 DAVLSL010000000
VERSION     DAVLSL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21907
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.019"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            75..2297
                     /gene="recJ"
                     /locus_tag="VXN30_000579"
     CDS             75..2297
                     /gene="recJ"
                     /locus_tag="VXN30_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1357246.1"
                     /translation="MITPTYEWQFALQVENADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2472..4133
                     /locus_tag="VXN30_000580"
     CDS             2472..4133
                     /locus_tag="VXN30_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1357247.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4202..4981
                     /locus_tag="VXN30_000581"
     CDS             4202..4981
                     /locus_tag="VXN30_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1357248.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5093..6313
                     /gene="murM"
                     /locus_tag="VXN30_000582"
     CDS             5093..6313
                     /gene="murM"
                     /locus_tag="VXN30_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1357249.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6318..7550
                     /gene="murN"
                     /locus_tag="VXN30_000583"
     CDS             6318..7550
                     /gene="murN"
                     /locus_tag="VXN30_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1357250.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7629..8330
                     /locus_tag="VXN30_000584"
     CDS             7629..8330
                     /locus_tag="VXN30_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1357251.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8370..10217
                     /gene="uvrC"
                     /locus_tag="VXN30_000585"
     CDS             8370..10217
                     /gene="uvrC"
                     /locus_tag="VXN30_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1357252.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGSLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10207..11040
                     /locus_tag="VXN30_000586"
                     /pseudo
     CDS             10207..11040
                     /locus_tag="VXN30_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11224..12024)
                     /locus_tag="VXN30_000587"
     CDS             complement(11224..12024)
                     /locus_tag="VXN30_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1357253.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12195..12800
                     /locus_tag="VXN30_000588"
     CDS             12195..12800
                     /locus_tag="VXN30_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1357254.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12813..14213
                     /gene="pepV"
                     /locus_tag="VXN30_000589"
     CDS             12813..14213
                     /gene="pepV"
                     /locus_tag="VXN30_000589"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1357255.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14267..14845
                     /locus_tag="VXN30_000590"
     CDS             14267..14845
                     /locus_tag="VXN30_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1357256.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15005..15157
                     /locus_tag="VXN30_000591"
     CDS             15005..15157
                     /locus_tag="VXN30_000591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1357257.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            15200..15292
                     /locus_tag="VXN30_000592"
     CDS             15200..15292
                     /locus_tag="VXN30_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1357258.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15463..16788
                     /gene="brnQ"
                     /locus_tag="VXN30_000593"
     CDS             15463..16788
                     /gene="brnQ"
                     /locus_tag="VXN30_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1357259.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16886..17923
                     /locus_tag="VXN30_000594"
     CDS             16886..17923
                     /locus_tag="VXN30_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1357260.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            17925..18386
                     /locus_tag="VXN30_000595"
     CDS             17925..18386
                     /locus_tag="VXN30_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1357261.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18404..19120
                     /locus_tag="VXN30_000596"
     CDS             18404..19120
                     /locus_tag="VXN30_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1357262.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19243..19668
                     /gene="rplK"
                     /locus_tag="VXN30_000597"
     CDS             19243..19668
                     /gene="rplK"
                     /locus_tag="VXN30_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1357263.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19876..20565
                     /gene="rplA"
                     /locus_tag="VXN30_000598"
     CDS             19876..20565
                     /gene="rplA"
                     /locus_tag="VXN30_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1357264.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(21157..21303)
                     /locus_tag="VXN30_000599"
     CDS             complement(21157..21303)
                     /locus_tag="VXN30_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357265.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVVDRTDEVSNHTHGKATLTWFEEI
                     FKEY"
     gene            21461..21679
                     /locus_tag="VXN30_000600"
     CDS             21461..21679
                     /locus_tag="VXN30_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1357266.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT         6591 a         4014 c         5047 g         6255 t
ORIGIN      
        1 caatctggat agagaaaacg tctaaatcat gttataatga agcaatagaa ttcttagaaa
       61 gagtggatgt ctttttgata acacctactt atgaatggca gtttgccctg caggtagaaa
      121 atgcggattt tacaaagata gccaagaagg ctggactggg tcctgaggtg gctcggttat
      181 tgtttgagag agggattcag gaccaagaaa gtctgaagaa gtttttagaa ccttccttgg
      241 aggacttaca tgatgcttat ctgctccatg atatggacaa ggcagtggag cggattcgtc
      301 aggctattga agaaggggaa aatattctcg tttatggaga ctatgatgcg gatggcatga
      361 cttcggcttc tattgtgaag gaaagtttgg aacaacttgg tgcggagtgc cgagtttacc
      421 tgccaaatcg ttttaccgat ggctatggcc ctaatgctag tgtttataaa tactttatcg
      481 agcaagaagg aatttccttg attgtgacgg tggacaatgg ggttgctggt catgaggcta
      541 ttgcattggc tcagtctatg ggagtagatg tcattgtgac agaccatcat tccatacctg
      601 aaaccctgcc agatgcttat gctattgtcc atcctgaaca tccagatgcg gattatcctt
      661 ttaaatattt ggctggttgt ggagttgctt tcaagttggc ttgtgccctg ttagaagaag
      721 tgcaagtgga attgcttgat ttggtcgcta ttggaactat tgcagatatg gtgagtctga
      781 cggatgaaaa tcgtatctta gttcaatatg gtctggaaat gttgggtcat acccagcgca
      841 ttggtctgca agaaatgctg gacatggctg ggattgctgc taacgaagta acagaagaaa
      901 cggttggttt ccagattgct cctcgtttga atgccttggg tcgcttggat gatcccaatc
      961 ctgccattga tttgttgact ggatttgatg atgaggaagc gcatgagatt gcccttatga
     1021 ttcaccagaa aaacgaagag cgcaaggaaa tcgttcagtc tatctatgaa gaagccaaga
     1081 ccatggtgga tcctgagaag aaggttcagg tcttggccaa ggaaggctgg aatcctgggg
     1141 ttctaggaat cgtggctggt cgtttattgg aagaattggg acagacagtc attgttctta
     1201 atatagaaga cggtcgtgcc aagggcagtg ctcgtagtgt ggaagcggtc gatatttttg
     1261 aagctctgga tccctatcga gacctcttca tcgcctttgg aggtcatgca ggtgcagcgg
     1321 gtatgacgct ggaagttgag caactctcag atttatctca gattttggaa gattatgttc
     1381 gtgaaaaagg tgcagatgct ggtggcaaga ataagttaaa cctagatgaa gagttggatt
     1441 tggaggcact tagcttggaa acggtcaaaa gttttgaacg tttagctcct tttggaatgg
     1501 ataatcagaa acctattttt tatatcaaga attttcaggt cgaaagtgct cgtactatgg
     1561 gggcaggtaa tgcccatcta aagctgaaaa tttccaaggg tgaggtgagt tttgaagtgg
     1621 tagcctttgg tcaaggcaga tgggcgacag agttttctca aaccaagaat ctagagttag
     1681 cggttaaatt gtctgtcaac caatggaacg gccaaactgc cctccagttg atgatgatgg
     1741 atgcgcgagt ggaaggtgtt caacttttta acattcgtgg aaaaaatgca gtcttgccag
     1801 aaggtgttcc agtcttggat tttcctggag aactgccaaa tcttgcggct agtgaagctg
     1861 ttgtcgtaaa aaacattcca gaggatatta ctcagctgaa gaccattttt caggaacagc
     1921 atttctctgc tgtctatttc aaaaatgata ttgacaaggc ctattatctg acaggttatg
     1981 ggactagaga tcagtttgcc aaattgtaca agactattta ccagttccca gagtttgata
     2041 ttcgctacaa gctgaaagat ttggctgcat atcttaatat tcaacaaatc ttgctggtca
     2101 agatgattca agtatttgaa gaactaggct ttgtgacgat aaaagatggt gtgatgacag
     2161 tcaataaaga ggcgccaaag cgggagatag gagaaagtca aatttaccaa aatctcaaac
     2221 aaaccgttaa agaccaagaa atgatggcgc tgggtacggt gcaagaaatt tatgattttt
     2281 tgatggaaaa agagtagaag ttaggaaaga gttgggaaat caactctttt ttgaaaacag
     2341 accttcattt tgaaaatcat caaaaaaatg gtataatggt aggaaaagat tcggctgaaa
     2401 gtattagaac ttttagaata agagggtaga gttgccctat aatcaagata aactaagatt
     2461 ttggaggaaa aatgagtaat atcagtttaa caacacttgg tggtgtgcgt gagaatggaa
     2521 aaaatatgta cattgctgaa attggagagt ccatttttgt tttgaatgta gggttaaaat
     2581 atcctgaaaa tgaacaatta ggggtcgatg tggtgattcc aaacatggat tacctttttg
     2641 aaaatagcga ccgtattgct ggggttttct tgacccacgg gcatgcggat gccattggtg
     2701 ctctacctta tctcttggca gaggctaaag ttcctgtatt tgggtctgag ttgaccattg
     2761 agttggcaaa gctctttgtc aaaggaaatg atgccgttaa gaaatttaat gatttccatg
     2821 tcattgatga gaatacggag attgattttg gtgggacagt ggtttccttc ttccctacga
     2881 cttactccgt tccagagagt ctgggaattg tcttgaagac atcggaagga agcatcgttt
     2941 atacaggtga cttcaaattt gaccaaacgg ctagtgaatc ttatgcaact gattttgctc
     3001 gtttggcaga gattggtcgt gacggcgtcc tggctctcct cagtgattcg gccaatgcag
     3061 acagcaatat tcaggtggct agtgaaagtg aagttaggga tgaaattacc caaactattg
     3121 ctgactggga aggtcgtatc atcgttgcag ctgtttccag taatctttct cgtattcagc
     3181 agatttttga cgctgcggat aaaacaggtc gacgtatcgt cttgacagga tttgatattg
     3241 aaaatatcgt ccgcacagcg attcgtctta agaagttgtc tttagccaac gaaattctct
     3301 tgattaagcc taaagatatg tctcgctttg aagaccatga gttgattatt cttgagacag
     3361 gtcgtatggg tgaacctatc aatggacttc gtaagatgtc gattggtcgc catcgttatg
     3421 tagaaatcaa ggatggggac ctggtctata ttgctacggc tccgtctatt gctaaagaag
     3481 cctttgttgc gcgtgtggaa aatatgattt atcaggcagg tggggttgtc aaattgatta
     3541 cccaaagttt acatgtatca gggcacggaa atgtgcgtga tttgcagctg atgatcaatc
     3601 ttttgcaacc taagtacctc ttccctgtcc aaggagagta tcgtgagttg gatgctcacg
     3661 ctaaggctgc catggcagtt gggatgttgc cagaacgcat cttcattcct aaaaagggga
     3721 cgaccatggc ttacgagaat ggagactttg ttccagctgg atcggtttca gcaggagata
     3781 tcttgattga tgggaatgcc attggtgatg ttggaaatgt tgttcttcgt gaccgtaagg
     3841 tcttgtcaga ggatggaatt ttcatcgtgg ctattacagt caaccgtcgt gagaagaaaa
     3901 ttgtggctag ggctcgtgtt catacgcgtg gatttgttta tctcaagaag agtcgcgata
     3961 ttctacgtga aagttcagaa ttgattaacc aaacggtaga agattatctt caaggagatg
     4021 actttgactg ggcagatctt aaagggaagg ttcgagataa tttgaccaag tatctctttg
     4081 accaaaccaa gcgtcgtcca gctattttac cagtagtcat ggaagcaaaa taatcgttga
     4141 aataaacaga gagaaagtcg agtttcggct ttttcttata gaaaaataga aggagaaaat
     4201 catggcagta atgaaaatcg agtattactc acaagtattg gatatggagt ggggggtgaa
     4261 tgtcctctac cctgatgcca atcgagtgga agaaccagag tgtgaagata ttcccgtctt
     4321 gtaccttttg cacgggatgt ctggaaatca taatagttgg cttaagcgga ccaatgtaga
     4381 acgcttgctt cgaggaacta atctcatcgt tgttatgccc aataccagca atggttggta
     4441 taccgatacc cagtatggtt ttgactacta cacggctcta gcagaggaat tgccacaggt
     4501 tctgaaacgc ttcttcccta atatgactag caagcgtgaa aagaccttta tcgctggtct
     4561 ttctatggga ggctacggct gcttcaaact ggctcttgcg acaaatcgtt tttctcatgc
     4621 agctagtttt tcaggtgccc tcagctttca aaacttttct cctgaaagtc aaaatctggg
     4681 aagtccagcc tactggagag gtgtttttgg agagattaaa gactggacaa ctagtcccta
     4741 ttctcttgaa agtctggcta aaaaatcgga taaaaagacc aaactttggg cgtggtgtgg
     4801 cgaacaggat ttcttgtacg aagccaataa tctcgcagtg aaaaatctca aaaaactagg
     4861 ttttgatgtg acctatagcc atagcgctgg aactcacgag tggtactact gggaaaaaca
     4921 attggaagtt tttttaacaa ccctaccaat tgatttcaaa ttagaagaga gactgactta
     4981 gtttgaactt cagcataggg ggagtagaac taaaataaaa tatgttttca ctagactttt
     5041 caaacgaaag tagtagaata gtaataaaat actggaggaa agagagtagg aaatgtaccg
     5101 ttatcaaatt ggcattccca cattagaata tgatcagttt gtcaaagaac atgaattagc
     5161 caatgtatta caaagtagtg cttgggagga agttaagtct aattggcaac atgagaagtt
     5221 tggtgtttac agggaagaaa aattactggc gacagctagt attttgatta gaactcttcc
     5281 gctaggctat aaaatgtttt acatcccaag aggacctata ttggattatg gggataaaga
     5341 actcttgaat tttgccattc agtctattaa gtcctatgct cgcagtaaga gagcggtttt
     5401 tgtgactttt gacccaagta tttgcctatc tcaaagttta atcaatcagg aaaagacaga
     5461 atttcctgaa aatctggcta ttattgatag tttgcaacaa atgggagtaa ggtggtcagg
     5521 aaaaacggag gaaatgggag acaccattca acctcgtatt caggcgaaaa tatacaagga
     5581 aaattttgaa gaagataaac tttccaagtc aacaaaacag gctattcgaa cagcacgaaa
     5641 caaagggctt gagattcaat atggtggact ggaactatta gattcatttt cggagttgat
     5701 gaaaaaaact gagaagcgaa aagagattca tttgaggaat gaagcctatt ataaaaaatt
     5761 gttagataat tttaaggaca aggcctatat caccttggcc accttggatg tttctaaacg
     5821 ttcgcaagag ttagaagaac agttagcgaa aaatagagcc ttggaagaga cctttactga
     5881 gtcgactcga acttcaaaag tagaagcgca gaagaaggaa aaagaacgtt tgttagagga
     5941 attgaccttc ttgcaggaat atatagatgt aggtcaagcg agagttcctt tagcggctac
     6001 tttgagtttg gaatttggta ctacctctgt caatatatat gctggtatgg atgatgattt
     6061 taaacgttac aatgcaccaa ttttaacttg gtatgaaacg gctcgctatg cctttgaacg
     6121 aggtatgatc tggcaaaatt taggtggtgt tgaaaactct ctcaatggtg gactttatca
     6181 ttttaaggaa aaatttaatc caacgattga agaatacttg ggtgaattta caatgcccac
     6241 tcatcctctc tatcctctgt taagacttgc tcttgatttc cgtaaaacat taagaaaaaa
     6301 acatagaaag taagtatatg gcactaacaa cactcacgaa agaagagttt cagacttatt
     6361 ctgatcaggt ttcttctcgt tcctttatgc aatctgtcca gatgggggat ttgctagaaa
     6421 aaagaggggc tcgaattgtt tatcttgctt tgaaacaaga aggagaaatt caagttgcag
     6481 ctctggttta tagcctgccc atgctgggtg gtctgcatat ggaactcaat tcggggccga
     6541 tttataccca acaagatgct cttccagttt tttatgcaga gttaaaagaa tatgccaagc
     6601 aaaatggtgt attagagttg cttgtaaaac cctatgaaac ttatcaaact tttgatagcc
     6661 aaggtaatcc aatagatgct gagaaaaaaa gtattattca agatttgact gatttaggtt
     6721 atcaatttga tggcttaaca acaggttacc caggtggaga accagattgg ttatactata
     6781 aagatttaac tgaattaact gaaaagagtt tgcttaaaag ttttagcaaa aagggtaaac
     6841 ccttggtgaa aaaggctgaa acctttggca ttcggttgaa aaagttaaaa cgtgaagaac
     6901 tatcgatttt taagaatata acaaaagaaa cctctgaacg tagagaatat agtgataaaa
     6961 gtttagaata ttatgagcat ttttatgata cttttggaga acaagcggag tttctcatag
     7021 caagcttaaa tttttcggac tatatgagca aattgcaagg tgaacaaagt aaactagaag
     7081 aaaacttgga caagttgcga cttgatttga gtaaaaatcc tcattctgag aaaaaacaaa
     7141 atcaactgag agaatattct agtcaatttg aaacgtttga agttcgaaaa gcagaagcgc
     7201 gagacttgat tgaaaaatat ggagaagaag atattgtttt agctgggagt ttatttgttt
     7261 atatgcctca ggaaacgact tatctcttta gtggttccta cactgagttt aataagttct
     7321 atgcccctgc actgcttcaa aaatatgtta tgttggaaag cataaaacgt ggaataccta
     7381 aatacaactt cctaggcatt caagggattt ttgatggaag tgatggtgtt ttgcgtttta
     7441 aacagaattt taatggctat attgtacgca aagcaggtac tttccgttac catccatcgc
     7501 ctttaaaata caaagctatc cagttactca aaaaaatagt aggacgttaa gatgaaaaag
     7561 tcagtattta gatttctttt agcttctttt agtaaaataa ttcttatttg ctagaaaggt
     7621 ggagagacat gcgctggctt tttcgtttga taggggcttt cttttctttt gtgtggcgtt
     7681 tgttttggcg tctggtttgg atagttgtgc tcttatgtgt gcttgctttc ggacttctct
     7741 ggtatctgaa cggagatttt caaggagcgc taaagcaagc agaacggtca gtaaaaattg
     7801 gtcaacaaag tattgaccaa tgggagaaaa cagggcaact gcctaagtta agccagacag
     7861 atagtcacca gcattctgaa ggaaggtggg cacaggcctc tgctcgtatt tacctggatc
     7921 cgcagatgga ttcacgcttt caagaggctt atttagaagc aatccagaac tggaatcaaa
     7981 ctggtgcttt taactttgaa ctcgtgactg agtctagtaa ggcggatatt acggctacgg
     8041 agatgaacga cggaggcact cctgtggcag gagaggcgga aagtcaaact aatctcttaa
     8101 cagggcaatt cttgtccgta acggtgcggt tgaatcatta ttatttgtcc aatccatact
     8161 atggctactc ctatgaacgc cttgtccata cggcagaaca tgagttaggt catgcgattg
     8221 gcttggacca tacagatgag aagtctgtca tgcaaccagc aggttccttt tatggtatcc
     8281 aggaagagga tgttgcaaac ctccgaaaaa tatatgagac tagtgagtag ggtactatct
     8341 ttccctactt tttttgctat aatggaacta tgaacaactt gattaaatca aaactagagc
     8401 tcttgccgac cagccctggt tgctacattc ataaggataa aaatggcacc attatctatg
     8461 taggaaaggc taaaaatctg cgtaatcgag tacggtccta ttttcgtgga agtcatgata
     8521 ccaagacaga ggctctggtg tctgaaattg tggattttga atttattgtt acggagtcta
     8581 atattgaggc acttctccta gaaatcaacc tgatcaagga aaacaagccc aagtacaata
     8641 tcatgctcaa ggatgacaag tcctatcctt tcatcaaaat caccaatgag cgctatccac
     8701 gcttgattat cactcgtcag gtcaaaaagg acggaagtct ttattttgga ccctatcccg
     8761 atgtgggggc agccaatgaa atcaagcggt tgctggatcg gatattccct tttcgtaagt
     8821 gtaccaaccc gccctctaag gtctgttttt attaccatat cggtcaatgc atggcccaca
     8881 ccatctgtaa gaaggatgag gcttatttca agtctatggc ccaggaggtt tctgattttc
     8941 tgaaagggca ggataacaaa atcattgatg agctaaaggg caaaatggca gcggcagccc
     9001 agactatgga gtttgaacgt gcggcggaat accgtgacct gattcaggct attggaacgc
     9061 ttcgaaccaa gcaacgggtc atggctaagg atctccaaaa tcgcgatgtc tttggctact
     9121 atgtggataa gggctggatg tgtgtgcagg ttttctttgt ccgtcagggt aagctcatcg
     9181 agcgcgatgt caatctcttc ccctacttca atgatccaga tgaggatttt ttgacctatg
     9241 taggacaatt ctatcaagaa aaatcccatc tagttcccaa tgaggtactg attccgcaag
     9301 atattgacga agaggctgtc aaggccttgg tggattccaa gattcttaag cctcaacgtg
     9361 gagagaaaaa gcagctggtc aatttagcta taaaaaatgc ccgtgttagc ctagagcaga
     9421 agttcaacct gctagagaaa tctgtcgaga agacccaagg agctattgaa aatctaggac
     9481 gcttgcttca aatcccaacc ccagttcgca tcgagtcctt cgataactcc aatatcatgg
     9541 gaactagtcc tgtttcagcc atggtggtct ttgtcaacgg caagcctagt aagaaggatt
     9601 accgtaagta taaaatcaag accgtagtcg ggccagacga ctatgccagt atgcgagagg
     9661 tcattcgcag acgctatggt cgagtacagc gtgaggcttt gactcctcca gatttgattg
     9721 tgattgatgg ggggcaaggt caagtcaata ttgctaagca agtcatccaa gaagaactgg
     9781 gcttggatat tccaattgca ggtctgcaaa agaatgataa gcaccaaacc catgaattgc
     9841 tctttggaga tccgctggaa gtggtggact tgtctcgcaa ttctcaggaa tttttcctcc
     9901 tccaacgcat tcaagatgag gtccaccgct ttgccattac cttccaccgc caactgcgct
     9961 ccaaaaattc tttctcatct caattggatg ggattgatgg tctgggacct aaacgcaagc
    10021 aaaatctcat gaggcacttc aagtctctga ccaaaatcaa ggaagccagt gtggatgaga
    10081 ttgtcgaagt tggagtacca agagcagtag cagaggctgt gcaaaccaag ttgaacccgc
    10141 aggaaacaga aatcttgctt caagtggcgg aagaaagagt agattaccaa acggaaggaa
    10201 accacaataa accataaaat cgcaatttta tcagatgttc atggcaatgc gacggcgcta
    10261 gaagcagtga ttgcagatgc taaaactcaa ggagccagtg aatattggct catgggagac
    10321 attttcctcc ctggtccagg tgcaaatgac ttggtcgctc tgttaaagga acttcctatc
    10381 acggcaagtg ttcgaggcaa ttgggatgat tgtgtccttg aggctttaga tgggcaatat
    10441 ggcttagaag acccacagga agttcagctc ttgcgtatga cacagtattt gatggagcga
    10501 atggatcctg caacgattgt ctggctacga agcttgcctt tgctggaaaa gaaagaaatt
    10561 gacggattgc gcttttctat ctctcataat ttacctgaca aaaactatgg tggtgacttg
    10621 ctagttgaga atgatacaga gaaatttgac caactgctag atgcggaaac ggacgtggca
    10681 gtttatggtc atgttcacaa gtagttgctt cgttatggta gtcaagggca acaaatcatc
    10741 aatccagggt cgattggcat gccctatttt aattgggagg cgttaaaaaa tcaccgtgcc
    10801 cagtatgccg tgatagaggt tgaagatggg gaattggtaa atatcctatt tcgtaaagtc
    10861 gcttatgatt acgaggctga gttagaattt gccaaatcca agggttttcc ctttatcgaa
    10921 atgtatgaag aactacgtcg tgaagataac tatcagaggc acaatctgga actattagca
    10981 agcttaatag aaaagcatag gtatgtagag gatgtgaaga atttttttga ttttttgtaa
    11041 gagtttccta aaataaccaa tgcaaactaa aaaagcgatt ggctgatccc atcgctttta
    11101 atatatctta tactcaatat gaaaatcaaa gagcaaacta ggaagctagc cgtaggttgc
    11161 tcaaagcaca gctttgaggt tgcagataaa gctgacgtgg tttgaagaga ttttcgaaga
    11221 gtgttattgt aactgagatt gatctgggag gtaagaacca cctagatagg tattgctgag
    11281 tttttcaagg gttccgtctt gatagagttc tttgagcgct ttatcaaatt gctctttaaa
    11341 ctctttttgg tcgcttgaga aaatgatata attgctgggg ctatctgcag aaggtaaatc
    11401 aacgactgag aggtctaaac cacggtcctt gataatcttt tgaacggata ccttgtcaaa
    11461 aactaggaaa tcaaactctc cgttagcaag gtctaggatt cgtttaccaa tatcctcacc
    11521 agaaaaatta attgtagcgg gattatcagt gtgtttctga ttccagttat tgatgaattg
    11581 agcgttagaa gttccggtat cctcttgtgt tgttttacca gcgatctggt caagagaagt
    11641 caaaggattt ttcttgttgc tgacaaggac gaggggattg ttggaaatcg gaagcgagta
    11701 aaggtatttt tcagcacgct cttttgtgta actcaaatta ttggccgcag cctgatagtg
    11761 accagaatca agtcctggga agatactttc ccaggcggtt ctttgaaatt gaatctcgta
    11821 gtcgctgagt ttttcatcta ctgcctttag aacttcgata tcgaagcctg tcagattgcc
    11881 tttatcttcg tagtcaaatg gtggcacgtc gccagctgta gcaaggacga ttgtcttttg
    11941 agcgctagtc tctttgggtg tagcttgatt cccacaggca accaaaaatg gtaggatagc
    12001 tagtaatagg ctaaattttt tcatactgtc tccattcaaa tgtaaagtac ttgctagttt
    12061 atcagaaact ttcctgataa accaatatat atcttttatg tctgtgatag aaaaacttaa
    12121 tcatgaaaaa tccctgcaag ctctttcaaa ttatggtaga atggaagcag tagaattaga
    12181 aaaggaaatc gattatgaaa tttcttgaat taaataaaaa acgtcatgcg accaagcatt
    12241 tcactgataa gcctgttgat ccaaaagatg tgcgtacggc tatcgaaatt gcaaccttgg
    12301 cgccaagcgc ccacaacagc cagccttgga aatttgtagt tgttcgtgag aaaaatgctg
    12361 aactggcaaa gttggcttat ggttcaaact ttgagcaggt atcatcagca cctgtaacca
    12421 ttgccctatt tacagataca gaccttgcca aacgcgctcg taagattgcc cgagttggtg
    12481 gtgctaacaa tttctcagaa gaacaacttc aatacttcat gaaaaatttg ccagctgaat
    12541 ttgcacgcta caatgaacaa caagtcagcg actacctagc tctcaatgca ggtttggttg
    12601 ccatgaactt ggttcttgca ttgacagacc aaggaattgg ttctaacatt attcttggtt
    12661 ttgacaaatc aaaagttaat gaagttttgg aaatcgaaga ccgtttccgc ccagaactct
    12721 tgatcacagt gggttataca gacgaaaaat tggaaccaag ctaccgcttg ccagtagatg
    12781 aaatcatcga gaaaagatag aaagaagaaa aaatgacagc aattgatttt acagcagaag
    12841 tagaaaaacg caaagaagac ctcttggctg acttgtttag ccttttggaa atcaattcag
    12901 aacgtgatga cagcaaggct gatgcccagc atccatttgg gcctggtcca gtaaaagcct
    12961 tggagaaatt ccttgaaatc gcagaccgcg atggctaccc aactaagaat gttgataact
    13021 atgcaggaca ttttgagttt ggtgatggag aagaagttct cggaatcttt gcccatatgg
    13081 atgtggtgcc tgctggtagc ggttgggaca cagaccctta cacaccaact atcaaagatg
    13141 gtcgccttta tgcgcgcggg gcttcggacg ataagggtcc tacaacagct tgttactatg
    13201 gtttgaaaat catcaaagaa ttgggtcttc caacttctaa gaaagttcgc ttcatcgttg
    13261 gaacagacga agaatcaggc tgggcagaca tggactacta ctttgagcac gtaggacttg
    13321 ccaaaccaga tttcggtttc tcaccagatg ctgaatttcc aatcatcaat ggtgaaaaag
    13381 gaaatatcac ggaatacctc cactttgcag gagaaaatac aggtgttgcc cgtcttcaca
    13441 gctttacagg tggtttacgt gaaaatatgg taccagaatc agcaacagca gtcgtttcag
    13501 gtgacttggc tgacttgcaa gctaaactag atgcctttgt tgcagaacac aaacttagag
    13561 gagaactcca agaagaagct ggcaaataca aggtgacgat cattggtaaa tcagcccacg
    13621 gtgctatgcc tgcttcaggt gtcaatggcg caacttacct tgccctcttc ctcagtcagt
    13681 ttggctttgc tggtccagcc aaagactacc ttgacatcgc aggtaaaatt ctcttgaacg
    13741 atcatgaggg tgaaaatctt aagattgctc atgtggatga aaagatgggt gctctttcta
    13801 tgaatgccgg cgtcttccgc ttcgatgaaa caagtgctga taataccatt gccctcaaca
    13861 tccgctatcc aaaaggaaca agtccagaac aaatcaagtc aatccttgaa aacttgccag
    13921 ttgtttctgt tagcctgtct gaacacggtc acacgcctca ctatgtgcca atggaagatc
    13981 cacttgtgca aaccttgttg aatatctatg aaaaacaaac tggctttaaa ggtcatgaac
    14041 aagtcatcgg tggtggaacc tttggtcgct tgttagagcg cggagttgcc tatggtgcta
    14101 tgttcccaga ctcaattgat accatgcacc aagccaatga atttatcgcc ttggacgatc
    14161 tcttccgagc agcagcaatc tatgccgaag ctatttacga attgatcaaa taaaacgata
    14221 gaagtctgag atcttatgct tggacttctt tttggaggga aagtagatgt ctcaaatcga
    14281 aagaatcaaa caggctatca tggcggattc acagaatgcc agctatacag agcgtggcat
    14341 tgagcctctc tttgcagcgc caaaaactgc tcgcatcaat atcatcggtc aggctccggg
    14401 acttaaaact caagaagcag gcctttactg gaaagataaa agtggtgacc gcttgcggga
    14461 ctggctaggt gtggatgaag atacctttta caattcaggt tattttgctg ttttgcctat
    14521 ggatttctac tttccaggac atggcaagtc gggtgatctt ccgcctcgta caggttttgc
    14581 agaaaaatgg catccgcagc tcttacagga attgcctgat attcagttaa ccctcttgat
    14641 tgggcaatat gcccaagcct actatttaca ggagaaaatc agtgggaagg taacggagag
    14701 ggtgaaacac tataaagact atctgccagc ctattttccg ctagttcacc catcaccacg
    14761 aaatcaaatc tggatggcca aaaatccttg gtttgaggca gaagtagtgc cagatttgaa
    14821 aaaaagaatt aaaaccattt tatagtcaat gaaaatcaaa gagcaaacta ggaagctagt
    14881 cgtaggctgc tcaaagtaca gctttgaggt tgcagataaa actgacgaag tcggtaacat
    14941 acgcacggta aggcgacgct gacgtggttt gaagagattt tcgaagagta ttagaagaaa
    15001 aagaatgaaa gaaatagcct ttgacgcatt ttaccagctt taccaaaacg accagctttc
    15061 tctagtggat gtgagagaag tggatgagtt tgcagctctt catttagaat gtgcccacaa
    15121 cctaccgctt agtcaattgg ctgatagtta tgattaattg gacaaagatc gcttgcatta
    15181 tattatttgc aaatctggaa tgagatcggc gcgtgcttgc caattcctgt tagaacaagg
    15241 ttataatgtt atcaatgtcc agggtggcat gttagccttt gaagaacttt aaaattttgc
    15301 atttctccta cttggtgtgg actgggtagg agagttttat ttttagataa ttcttatttt
    15361 taagaaaatt gaaaacattt aatatttgcc tcgtgatgct tttttcagac tcctaatcgt
    15421 ggtatactag gtcagtattt tataaatatg aaggagattt ttatggctaa aaaaggtacc
    15481 ctaacaggtt tgctcctgtt tggaatattt tttggtgcgg ggaacttgat ttttccgcct
    15541 tctctaggtg ctctatctgg agaacatttt cttcctgcca tcgcaggttt tgtcttttca
    15601 ggcgttggta tcgccgtctt gacccttatt attggaacgc taaatcctaa aggatatatc
    15661 tacgagattt caacgaagat agcgccttgg tttgcgactc tttacctctc agttctttac
    15721 ttgtcaatcg gtccattctt tgctacccca cgtactgcta caacagctta cgaagtaggg
    15781 attagccccc ttttgtcgga tgcaaataaa ggacttggct tgattgtatt tacggttctg
    15841 tattttgcgg cagcctattt gatttcgctt aatccatcaa aaatcttaga ccgcattgga
    15901 cgtgttttaa cgccagtctt tgcaattttg attgttatct tggtcgttct gggagctatc
    15961 aaatatggtg gaacaagtcc tcaagctgct tcagctgctt atcaagcttc tgcctttggt
    16021 acaggtttcc tagaaggtta caataccttg gacgcccttg cttcagtggc ctttagcgta
    16081 atcgcagttc aaaccttgaa acaacttgga ttttcaagta agaaagaata catttcaact
    16141 atttgggttg ttggtatcgt tgttgccctt gccttcagcg ctctttacat cggtttaggt
    16201 tttcttggaa atcatttccc agtaccagct gaagcgatga agggtggaac accaggtgtt
    16261 tacatcttgt cacaagccac tcaagaaatc tttggctcaa cagctcaact cttccttgca
    16321 gctatggtta ccgtaacctg cttcacaacg actgttggtt tgattgtgtc aacagctgag
    16381 ttctttaatg agcgcttccc acaaatcagc tacaaggttt atgcgacagc ctttaccttg
    16441 attggatttg ctattgccaa tttgggtctt gatgcgatta tcaagtactc aattccagta
    16501 ctggttatct tgtacccaat cacgattgct atcgttatga ttgtcattgt caacaaattt
    16561 gttgcccttt caaaaccagg tatgcagttg acaattgctg tggttacagt tattgccatt
    16621 gcaagcgtac taggaagctc gtttaaggtt gagtttcttg caaatcttgt tagcgttctt
    16681 ccttttgcca aggcatctct cccatggttg gtgccagcca ttgttggaat cttgctctca
    16741 ttggttctac caaacaagca agaaagcgat gtttttgaaa tggaataatc acttaaatca
    16801 cttttgtagc caagtctaca ggagtgattt tcttttttta tccgatgata aatgtgttat
    16861 aataggtagc gaaagaggtg aagaaatgaa tcaaacagta gaatatatca aagaactgac
    16921 agccatcgca tcgccaacgg gcttcactcg tgaaatttcg aattatttag tcaagactct
    16981 agaaggtttt ggttaccagc cagttcgcac agctaagggt ggtgtcaatg tgaccatcaa
    17041 aggtcaaaat gatgagcaac atcgctatgt gactgcccat gtggatacgc tgggtgctat
    17101 tgttcgtgct gtcaaaccag atggtcgtct taaattggac cgtatcggtg gttttccttg
    17161 gaacatgatt gaaggtgaaa actgtaccgt tcatgtggct agcacaggtc aaaaggtatc
    17221 aggaaccatc ctcatccacc aaacttcttg ccatgtctac aaggatgcag gaactgcaga
    17281 acgcacgcag gacaatatgg aagtgcgttt ggatgtaaaa gtaagcaatg aaaaagaaac
    17341 tcgtgctctt gggattgagg tcggtgattt tatcagcttc gatacacgaa ctgttgtgac
    17401 tgatacaggt tttatcaagt ctcgtcattt ggatgataag gtcagcgcag cgattttgct
    17461 caacctgctt cgtatttata agaaagagaa gattgaattg ccagtaacaa ctcattttgc
    17521 tttttcagtt tttgaagaag taggacatgg tgctaattct aacattcctg ctcaggtagt
    17581 agaatatctg gctgtggata tgggagctat gggagatgac cagcagacag atgagtacac
    17641 agtatctatc tgtgtcaagg atgcttcagg tccttatcac tatgacttcc gtcaacactt
    17701 ggttgccttg gctaaagagc aagatattcc atttaagctc gacatctatc cattttacgg
    17761 atcggacgct tcagcagcga tgtcagcagg tgcagaggtc aagcatgcac ttcttggagc
    17821 tggtattgag tccagtcact cttatgagcg cacccacatc gattcagtaa tagcgactga
    17881 gcgtatggtt gatgcttatc ttaagagcga ggcggtatca tagaatgtgc ttaatctgtc
    17941 agagaatcga actcattaaa gctgggcaaa atccctactt tgtaaaagaa ctagaaacag
    18001 gctatgttgt tattggtgac taccaatact ttaagggcta taccttattt ttagcaaaag
    18061 atcatgtcac agaactccat catatggaaa cttctgtaaa acttcgcttt ttagaggaaa
    18121 tgagtttagt ccaagaagca gtcgccaaag catttgaggc tgaaaagatg aatattgaac
    18181 tcctaggaaa tggcgatgcc cacgctcatt ggcatctttt tccccgaaga gcaggtgata
    18241 tgaagagtca cggtttgaat ggccgtggcc cagtctggtg ggtgccctgg gaagaaatgg
    18301 cggcagaaga ttgccaagta caatcgccag agctggaaga gatgattaaa atcttatctc
    18361 atgaattgga gaagtatttg gcctaagaga gaggaagaaa gaaatgaaaa aaagatatgt
    18421 cattttatct ggtttactag cattgaccct tgcagcttgt tcgcaagaaa aaaccaaagt
    18481 tgaagaaaac actcaaaaaa cggagcaaag cagtcaaccg gaaggaactg taggtagcaa
    18541 atctcaagct tctagtcaga aaaaggcaga agttagcaat aaagggagct attatagtat
    18601 ccaagggaaa tacgatgaga ttatcctagc taataaacgt tatcctttgt caaaagatta
    18661 caatccagga gaaaatccaa cagccaaggc agagttgctc aaactaatcg cagctatgca
    18721 ggctgagggc tatccaatca gtgaccaata cagtgggttt aggagttatg aaactcaggc
    18781 taagctctac caagactatg tcaatcaaga tggtaaggaa gcagcagatc gctactcagc
    18841 acgcccaggt tacagtgaac accagacggg tcttgctttt gatttgattg ggacagatgg
    18901 ggatttagtt actgaagaaa aggcagccca gtggctcttg gaccatgcgg ctgactatgg
    18961 ctttgttgtt cgctatctta aaggcaagga aaaggagact ggctacatgg cagaagaatg
    19021 gcatctccgc tacgttggaa aagaagccaa agaaattgct gcaagtggtc tcagtttgga
    19081 agagtactat ggctttgaag gcggcgatta tgtcgattaa aaatgaaagt tttctcttgc
    19141 atttttagat aaatagtgta taatggaaag gtatgtgtaa agcatacttg tgggaggtaa
    19201 aaatctctaa ttaccgccaa aaccacaaag gaggatttaa aaatggctaa aaaagtcgaa
    19261 aaacttgtaa aattgcaaat ccctgctggt aaagctacac cagctccacc ggttggacct
    19321 gctcttggtc aagctggtat caacatcatg ggattcacaa aagagttcaa cgctcgtaca
    19381 gctgaccaag ctggtatgat cattccagtt gttatctcag tttacgaaga taaatcattt
    19441 actttcgtta cgaaaacacc accagctgct gttcttttga aaaaagctgc aggtgttgaa
    19501 aaaggatcag gtacacctaa taaaactaaa gttgctacag ttactcgtgc acaagtacaa
    19561 gaaattgcag aaactaagat gccagatttg aacgcagcaa acgtagagtc tgcaatgcgt
    19621 atgatcgaag gtactgctcg ttctatggga ttcactgttg ttgactaatc aataacacag
    19681 tagaaaatct cacagcagtc tattagttgc ttttcatact aggcaagtga ctgaggcttg
    19741 tacttggata cagcaaggga gcttaaggcc gtaggaagag aaaaatagta gactgaaaac
    19801 ccgcaagact tcatcatttc gagaagtgac gtgggagatg aaaatcgatt gaaccactta
    19861 caaggagaat agaaaatggc taaaaaaagc aaacaacttc gtgctgctct tgagaaaatc
    19921 gacagcacaa aagcatacag tgtagaagaa gctgtagcac ttgcaaaaga aactaacttt
    19981 gcaaaatttg atgcaactgt agaagttgct tacaacttga acatcgacgt taaaaaagct
    20041 gaccaacaaa tccgtggagc aatggtattg ccaaacggta ctggtaaaac ttcacgtgtt
    20101 cttgttttcg cacgtggtgc aaaagctgaa gaagcaaaag ctgctggtgc agactttgtt
    20161 ggtgaagatg accttgttgc taaaatcaac gacggttggt tggacttcga cgtagttatc
    20221 gctacacctg atatgatggc tcttgttgga cgtcttggac gtgtccttgg accacgtaac
    20281 ttgatgccaa accctaaaac tggtactgta acaatggatg ttgctaaagc agttgaagag
    20341 tctaaaggtg gtaaaatcac ttaccgtgct gaccgtgcag gtaacgttca agcaatcatc
    20401 ggtaaagtat catttgaagc tgaaaaattg gttgaaaact ttaaagcttt caacgaaaca
    20461 atccaaaaag taaaaccagc tacagctaaa ggaacttacg taacaaactt gactatcaca
    20521 actactcaag gtgttggtat caaagttgac gtaaactcac tttaatcagt agttaaagta
    20581 atgtaaaaaa gttgaagaca ctatgtctca actttttttg atgtacgacg ggcatgtcgt
    20641 atatctgagg tgtaagtcct cggtgggcac ctactaccgg tgaacccaat agcgattccc
    20701 aagtctgacg ctggaaataa gaatcgtcag aggaagggat agcgaaatcg tggctctacg
    20761 aacaggaacg tgatagtaag gcctatatag cggataaggt atctggctgg ggcgaccact
    20821 atcaattata ataaaatgaa ataagaatag gacgaatcgt tcaggacagt caaatcgatt
    20881 tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatctact atagtagctc
    20941 agaagtcggt acttaaacgt gctatatcaa aaccagtcct tgaaaaacgt ggactggttt
    21001 cgtgtttgga ttattacctt gaacgacatg cgttaaaagt tagttgaacc gccgtatgcc
    21061 gaacggcacg tacggtggtg tgagaggggc tagagattat cccctactcg atttcgaaat
    21121 ctagtggaat gaatctggaa tagtccatcg agctttctaa tactctttga aaatctcttc
    21181 aaaccacgtc aacgtcgcct tgccgtgcgt atggttactg acttcgtcag ttctatccac
    21241 aacctcaaaa cagtgttttg agcaacctgc ggctagtttc ctagtttgct ctttggtttt
    21301 cattgagtat aacacattgt tagaagttgg tttaaatttc ctaatcagtt tgttcacatt
    21361 taccttcgat atattatatc ccatagttaa ggttggtcat acagatgatt atagccatgg
    21421 agccgtaaaa cttagtgttt ctttagttga caaagatgcc atgaaaaaaa tatctgtaac
    21481 tgtaatagga tattttgaaa taaatataga tgaaaatatc accgatattc tatacgtaaa
    21541 tggtactgct attctttatc cttatttacg ttctattgtt tcaatagttt cggcaattga
    21601 tagcagtgaa gcaatgttgc tacctaccat taatgtttta gagttactag ataaatctca
    21661 accttttgaa gaagaataat ttattagctc actaaattga gggtaaggaa aagtaaaagc
    21721 agtaagaaaa atgtcttgca ttatacagca actttttggg aatgagtaga tggattgaat
    21781 aaaatttgat taagagtgga tgatttatct gtagattatt attggacagt tagtcttgaa
    21841 gtagtctaag aattaggtta taatcagtag aagccttgct aataatgagg aggttagttt
    21901 atgtata
//
LOCUS       DAVLSL010000020        21377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLSL010000020 DAVLSL010000000
VERSION     DAVLSL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.020"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            43..264
                     /locus_tag="VXN30_000601"
     CDS             43..264
                     /locus_tag="VXN30_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1357267.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            781..1034
                     /locus_tag="VXN30_000602"
                     /pseudo
     CDS             781..1034
                     /locus_tag="VXN30_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1162..2391
                     /locus_tag="VXN30_000603"
     CDS             1162..2391
                     /locus_tag="VXN30_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1357268.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2411..5107
                     /gene="ppc"
                     /locus_tag="VXN30_000604"
     CDS             2411..5107
                     /gene="ppc"
                     /locus_tag="VXN30_000604"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1357269.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5190..5407
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5520..6524
                     /locus_tag="VXN30_000605"
     CDS             5520..6524
                     /locus_tag="VXN30_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1357270.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6593..7459
                     /locus_tag="VXN30_000606"
     CDS             6593..7459
                     /locus_tag="VXN30_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1357271.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7456..8214
                     /locus_tag="VXN30_000607"
     CDS             7456..8214
                     /locus_tag="VXN30_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357272.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8304..10064
                     /gene="dnaG"
                     /locus_tag="VXN30_000608"
     CDS             8304..10064
                     /gene="dnaG"
                     /locus_tag="VXN30_000608"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1357273.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10067..11176
                     /gene="rpoD"
                     /locus_tag="VXN30_000609"
     CDS             10067..11176
                     /gene="rpoD"
                     /locus_tag="VXN30_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1357274.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11190..11519
                     /locus_tag="VXN30_000610"
     CDS             11190..11519
                     /locus_tag="VXN30_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1357275.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11634..12677
                     /gene="cpoA"
                     /locus_tag="VXN30_000611"
     CDS             11634..12677
                     /gene="cpoA"
                     /locus_tag="VXN30_000611"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1357276.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12693..14018
                     /locus_tag="VXN30_000612"
     CDS             12693..14018
                     /locus_tag="VXN30_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1357277.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14049..14225
                     /locus_tag="VXN30_000613"
     CDS             14049..14225
                     /locus_tag="VXN30_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1357278.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14284..14418
                     /locus_tag="VXN30_000614"
     CDS             14284..14418
                     /locus_tag="VXN30_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1357279.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14482..15792
                     /gene="obgE"
                     /locus_tag="VXN30_000615"
     CDS             14482..15792
                     /gene="obgE"
                     /locus_tag="VXN30_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1357280.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15802..15960
                     /locus_tag="VXN30_000616"
     CDS             15802..15960
                     /locus_tag="VXN30_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357281.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17047..18306
                     /locus_tag="VXN30_000617"
     CDS             17047..18306
                     /locus_tag="VXN30_000617"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1357282.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18322..18873
                     /locus_tag="VXN30_000618"
     CDS             18322..18873
                     /locus_tag="VXN30_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357283.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            18866..20143
                     /locus_tag="VXN30_000619"
     CDS             18866..20143
                     /locus_tag="VXN30_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1357284.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20159..21019
                     /locus_tag="VXN30_000620"
     CDS             20159..21019
                     /locus_tag="VXN30_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1357285.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
BASE COUNT         6535 a         3676 c         4799 g         6367 t
ORIGIN      
        1 ttcttagttt tatatattat acttttctca ggagaaagct agatgtacag acgtttgaga
       61 gatttgaggg aagatcacga tttgacccaa aaacaaatag ctaaaatact ttcgtttaca
      121 aactcagctt atgctaaaat tgaacggggt gagcatacgt taacagcaga tgtattggta
      181 actctctcaa acttttacga tgtcagtaca gactacctat tggaattgac agattttcct
      241 gataaaattc gctttagaaa ataatctcct caatttcata gagtgtgaaa atgaggagat
      301 tttttatttg ttctttgaaa attaaatagt ccaaaatggt actttcctca tttgtggagc
      361 aaatttgaat ggctcgccat gataagagcg attttaaaac catcaataaa atagagcgat
      421 actttatatg ccatgataca aatgatatac aatgatactt ctgaccgttc aggctgccat
      481 cgtaaaagag cagcaagtga aattcttatg atgacttcat cagtcatgcc acgttaaatg
      541 tgtgagtttg ttagagaaac gcaattaatc ctcaaaaggt tccccgaacc ttttgagttc
      601 cacagacgca tcacgtggag tgtgtaagct tgttgctaaa agcgtaaaaa ccttggaacg
      661 aaaggaataa tagacttcct gcgaaacaaa aatacaatac aataaaacta tggatgatga
      721 agcaagtaaa caattaagcg atagccgttt caagagcctt gtaggtgttc agcgcacgag
      781 atgttagctg tgttaaaaac agcttatcaa cgcaacgtaa acgcgcaaaa ggtggacgaa
      841 aaaccaaatt aagcctagac gatctcctca tggtaactat tcaatacatg cgagaatagc
      901 gcacttatga acaaattgcg gctgattttg gcattcacga aagcaactta atccgtcgga
      961 gccaatgggt tgaagtaact cttgttcaaa atggttttac gatttcaaat tctgccctaa
     1021 ttatgaaaaa cagtaaaatt cgaaggattg taaggtaaga gtttttttct ttctgaaaaa
     1081 atggtataat agcaatcaaa actagaaaat aaaacggaat ttggaacaga tttgtctgta
     1141 tcctagtaga gtggtgatgc tatgaagatt agtaagaggc acttattaaa ttattccatc
     1201 ttgattccct acttgctttt atctattttg ggcttgattg tggtctattc gaccaccagt
     1261 gctattttaa ttgaagaagg caagagcgcc ttgcagttgg ttcgaaacca aggaatcttt
     1321 tggattgtta gtttgatact gattgcctta atttataaat tgagactaga ttttttgaga
     1381 aatgagcgac taatcatttt agttatatta atagaaatgc ttttattgtt cttggctcgt
     1441 tttattggta tttccgtaaa cggggcatac ggttggattt cggttgcagg agtaactatt
     1501 cagccagctg agtacttaaa aatcattatt atttggtatt tagctcaccg attctccaaa
     1561 cagcaagaag aaatagctac ttatgatttt caagttttga ctcaaaatca atggcttccc
     1621 cgtgctttta atgattggcg attcgttctc ctagttctga ttggaagttt gggaattttc
     1681 cctgatttag gaaatgcgac tattttagtc ttggtttcct tgattatgta tacagttagt
     1741 ggaatcgctt atcgctggtt ttcaaccatt ctggcgctcg tatctgccgc ttctgtcttt
     1801 gtcttgacca ctatcagcct aatcggtgtt gagacctttt caaaaattcc agtatttggc
     1861 tatgtagcca agcgctttag tgcctttttt aatccttttg ccgatcgtgc tgatgcaggt
     1921 caccagttag ctaattctta ttttgccatg gtcaatggtg gttggtttgg tctaggtctt
     1981 ggaaactcga ttgaaaaacg aggttatttg ccagaagctc atacagactt tgtcttttct
     2041 atcgtgattg aagaatttgg ctttgctggt gccagtctta ttttagctct cttgtttttc
     2101 atgattttgc ggattatctt ggtcggtatt cgagcggaga atcctttcaa tgccatggtt
     2161 gcactcggtg tcggagggat gatgttggtt caggtatttg tcaatatcgg agggatttcg
     2221 ggcttgattc catctacagg agtaaccttc cccttcttat cccagggtgg aaatagtctt
     2281 ctagtcttat cagtggcagt agcctttgtc ttaaatattg atgccagtga aaaacgcgct
     2341 aagttgtacc gagaattgga aaatcaacca atgaaccttc tgttgaagta ggataaagaa
     2401 aggatagttt atgtctcttc aaaaattaga aaattatagt aataaaagtg ttgtgcaaga
     2461 agaagtcttg attctaacag aattactgga agatattact aaaaatatgc ttgccccaga
     2521 gacctttgaa aaaataatac agttgaaaga attatcaacg caggaagatt atcaaggtct
     2581 aaaccgtcta gtgactagct tatcaaatga tgaaatggtc tatatttcac gctatttctc
     2641 tatcttacct cttttgatca atatttcaga ggatgtggat ttagcttatg aaatcaatca
     2701 tcaaaataat attgatcagg actatttagg taaattatct acaacgatta aattggtagc
     2761 agaaaaggaa aatgccgttg agatcctaga acacttgaat gttgtccctg ttttgacagc
     2821 ccatccaaca caagtgcaac gcaaaagtat gttggattta acaaatcata ttcatagtct
     2881 tttgcgtaaa taccgtgatg ttaagttggg gttgatcaat aaagataaat ggtacaatga
     2941 tttgcgtcgt tacatcgaaa ttatcatgca gacagacatg attcgtgaga aaaaattaaa
     3001 agtgactaac gaaatcacga atgctatgga atattataac agctcctttt tgaaagctgt
     3061 acctcatttg acgacggagt ataagcgctt agcgcaagcg catggtctga atttaaaaca
     3121 ggctaaacca atcaccatgg gtatgtggat aggtggtgac cgtgatggaa atccatttgt
     3181 tacagcagag accttgaagc agtctgcact cactcaatgt gaagtcatca tgaactacta
     3241 tgataaaaag atttaccaac tttatcgtga attttctctt tcaactagca ttgtcaacgt
     3301 cagcaagcaa gtcagagaaa tggctcgtca atccaaggat aactcgattt accgcgaaaa
     3361 agagctttac cgtcgtgcct tgtttgatat tcaatcaaaa attcaggcaa ctaaaaccta
     3421 tctgattgag gatgaagaag ttgggactcg ttatgaaacc gccaatgatt tctacaagga
     3481 tttgattgcc attcgagatt ctctactaga aaataagggt gagtccttga tttcaggtga
     3541 ttttgtggaa ttattgcagg cagtagagat atttggtttt tacctagcat caattgatat
     3601 gcgacaagac tctagcgtct atgaagcctg tgtggcagaa ctcttgaaat cagcaggaat
     3661 tcattctcgt tatagcgagt tgagcgaaga agaaaagtgt gaccttctct tgaaagaatt
     3721 agaagaagat ccccgaattc tttctgcgac tcacgcagaa aaatcagaat tattagcaaa
     3781 agaattagct atttttaaga cggctcgtgt tttgaaagat aagttgggag atgatgtcat
     3841 ccgtcagacc atcatttcac atgcaaccag cctttctgat atgctagaat tagctattct
     3901 gttaaaagaa gtaggactgg tggatacgga aagggcgcgt gttcagattg ttcccctttt
     3961 tgaaacaatt gaagacttgg atcattcaga agaaacaatg agaaaatatc tttctcttag
     4021 ccttgccaaa aaatggattg actcacgaaa taactaccaa gaaatcatgc ttggctactc
     4081 tgacagtaat aaagatggcg gttacttgtc atcatgttgg accctctaca aggctcaaca
     4141 acaattgact gctattggag atgaatttgg cgttaaggtt accttcttcc atggtcgtgg
     4201 tggtactgtc ggtcgtggtg gtgggccaac ctatgaagcc attacatctc aaccgctcaa
     4261 gtctatcaag gatcgtatcc gcttgacgga gcagggtgaa gtaattggga ataaatacgg
     4321 taacaaagac gccgcttact ataaccttga aatgctagta tcggcagcta ttaaccgtat
     4381 gattactcag aagaagagcg ataccaatac cccaaatcgt tatgaagcca ttatggatca
     4441 agtagtggac cgtagttacg atatctaccg tgatttggtc tttggtaatg agcatttcta
     4501 tgattatttc ttcgagtcaa gtccaatcaa ggctatttca agttttaata ttggttctcg
     4561 tccagccgct cgtaagacta ttactgaaat cggtggtttg cgtgccatcc cttgggtatt
     4621 ctcatggtca cagagtcgtg ttatgttccc tggatggtac ggggttggtt caagcttcaa
     4681 ggaatttatc aataaaaatc cagagaatat tgctatctta cgagatatgt accaaaattg
     4741 gcctttcttc caatcgcttc tttcaaatgt tgatatggtt ttgtcaaaat caaatatgaa
     4801 tattgctttt gaatatgcta aactttgtga agacgagcaa gttaaggcca tctatgagac
     4861 tattttaaat gaatggcaag ttactaagaa cgttatcttg gctattgaag gacatgacga
     4921 actcttagct gacaatccat atctaaaagc tagtctggat taccgtatgc cttactttaa
     4981 tattctcaac tatattcagt tagagttgat taaacgccaa cgtcgtggag aattgtccag
     5041 tgatcaagaa cgattgattc atatcaccat caacggaatt gcgacaggat tgcgtaattc
     5101 aggttgataa ttttcaagag tgaatgctaa aagtgaatat caaaaaaatt ctaatagact
     5161 attgacaagt agtttaaaaa tgatataatt taaccattca gaaaagtaat catacaaact
     5221 ttttagagag tctgtggtag ctgaaaacag ataagtggcg atgatgaaaa ttgggctgaa
     5281 tgctatttag aatttgaaat tataaaaatt cggtaagcac accttacagt gcatctcgtt
     5341 attgcgagac tgagcgatag ggaaattccc tataattgag gtggtaccgc gcatcgacgt
     5401 cctcacacaa gttttttgtg tgaggatttt tttgatggag gttagtatgg aaagaaaacg
     5461 atggcgtcgc ttgtttagat aagtgaaata tgttaaagga aataaaaagg agaaacagaa
     5521 tgaaaaataa acgtttaatt ggaattattg ctgcattagc agtcttagta gcaggaagct
     5581 tgatttattc ttcaatgaat aaatcagaag ctcagaataa taaggatgag aagaaaataa
     5641 ctaagattgg tgtgcttcaa tttgtgagcc atccatccct tgatttgatt tataaaggga
     5701 tccaagatgg acttgcagaa gaaggatata aagatgatca agttaaaatt gattttatga
     5761 actcagaagg tgaccaaagt aaggttgcga caatgagtaa acaattggtt gcaaatggga
     5821 atgaccttgt ggttggtatc gcaacaccag cagcccaagg gttggctagt gcaacaaaag
     5881 acctaccggt tatcatggcc gctattacag acccaattgg tgctaacttg gttaaagatt
     5941 tgaaaaaacc aggtggcaac attacagggg tatctgacca caatccagct caacaacaag
     6001 ttgaactcat caaggctctg acaccgaatg tgaaaacaat cggagctctt tactcaagta
     6061 gcgaagacaa ttcaaaaaca caggtcgaag aatttaaggc ttatgctgaa aaagcaggtc
     6121 tgacagtgga aacatttgca gttccttcaa caaatgaaat tgcctcaact gtcactgtta
     6181 tgactagcaa ggtagatgct atttgggttc caattgataa caccattgca tcaggatttc
     6241 caacggttgt ctctagcaat caaagttcta agaaaccaat ttatcccagt gcgacagcta
     6301 tggtagaagt aggtggtttg gcatcagttg taattgacca acatgacctt ggtgtggcaa
     6361 caggtaaaat gattgtgcaa gtcttgaaag gtgcaaaacc agccgatacc ccagtcaatg
     6421 tcttttcaac tggtaagtca gtcatcaata aaaaaatagc acaagaacta ggtattacta
     6481 ttcctgagtc tgttctcaaa gaagcaggac aagtcatcga ataaataagg tagggaggag
     6541 gagactcttc ccatttttta aaaagatgaa tatagaaagg attaaataaa atatgattgt
     6601 ttccattatt tctcaaggat ttgtctgggc tattctaggt ctgggaatct ttatgacatt
     6661 taggatttta aactttccag atatgacgac agaaggttcc ttccctcttg ggggagctgt
     6721 tgctgtcact ttgataacca aaggcgtgaa cccattttta gcgacacttg ttgctgtagg
     6781 agcaggttgt ttggctggaa tggcagcagg ccttctttat acaaaaggga agatcccaac
     6841 cttgctctca gggattttgg tgatgacttc ttgtcactca atcatgctct tgattatggg
     6901 acgtgcgaat ttaggcctgc ttggaaccaa gcaaattcag gatgttttgc cttttgattc
     6961 ggatttgaat caactcttga caggtctcat ctttgtgagt attgttattg ctctcatgct
     7021 ctttttcttg gacactaaac tcggacaagc ctatattgct acaggggata atcctgatat
     7081 ggctagaagt ttcgggattc atactggacg catggagctc atgggcttgg tcttatcaaa
     7141 tggtgtgatt gcccttgcag gtgccctcat tgctcagcaa gaaggttatg ccgatgtgtc
     7201 tcgagggatc ggggttatcg ttgtggggct tgcaagtttg attattggag aagttatttt
     7261 caagagtttg agcttggcag agcgtttggt tactatcgtt gtaggttcta tcgcttatca
     7321 atttttagtg tgggcagtta tcgcacttgg ctttaataca agttaccttc gtttatacag
     7381 tgccttgatt ttagcagtct gcctcatgat tccaacattt aagcaaacaa tcttgaaagg
     7441 agccaagtta agcaaatgac agcaattgta gaattaaaaa atgcaaccaa aatcgttaaa
     7501 aatggctttg atgaagaaaa gattatttta aatgatgttt ccttagaaat ttttgaacgg
     7561 gactttatca cgattttggg cggaaatggt gctggaaaat caaccctctt taacactata
     7621 gcagggacct tatcactaac tagtggaact atccgtattt taggtgaaga actcactaag
     7681 ttttcacccg agaagcgtgc caagtacctg tctcgtgtct tccaagatcc aaagatgggg
     7741 acagctcccc gtatgacggt cgctgaaaat cttttaatcg ccaagtttcg tggtgaaaag
     7801 cgtggattgt taccacgacg cttgactagc tataaggatg aatttcaggc aaccattgaa
     7861 aaagtaggaa atggtcttga gaaacacttg aatacaccga ttgagttctt atcaggtgga
     7921 caaagacagg ctttgagtct cttgatggca accttgaagc gacctgaatt actcctgtta
     7981 gatgagcata ctgctgccct ggatccaaag actagtgttg ctttgatgga attgacagat
     8041 gaatttgtta agaaagatca gctaacagcc cttatgatta ctcatcatat ggaagatgct
     8101 ctcaaatacg gcaatcgctt gattgtcatg aaagaaggac ggattatcca agatttaaac
     8161 caagaagaaa aagcaaaaat gaaaatctct gattattatc aactctttga ataaagtagg
     8221 gaaaatgagt gaaatggttt actttttttc tgaaataaag tatactatat aaagtaaact
     8281 atgataacat ggaggtattg tgtatggttg acaaacaagt cattgaagaa atcaaaaaca
     8341 atgccaacat tgtggaagtc ataggagatg tgatttcttt acaaaaggca ggacggaact
     8401 atctagggct ctgtcctttt catggtgaaa aaacaccttc tttcaacgtt gtagaggata
     8461 agcagtttta tcactgtttc ggttgtggtc gctcaggtga tgtctttaag ttcatcgagg
     8521 agtaccaagg ggttcccttt atagaggcag tccaaatctt aggtcagcgt gttggaattg
     8581 aggttgaaaa accgctttat agtgagcaga agccagcctc acctcaccaa gctctttatg
     8641 atatgcacga agatgcagcc aaattttacc atgcgattct catgacaacg actatgggcg
     8701 aagaggccag aaattacctt tatcagcggg ggttgacaga tgaagtgctt aaacattttt
     8761 ggattggttt agcacctcca gaacgaaact atctctatca acgtttgtct gatcagtatc
     8821 gtgaagagga tttactggat tcaggcctgt tttatctctc ggatgccaat caatttgtag
     8881 acacctttca caatcgcatt atgtttcccc tgacaaatga ccaaggaaag gtcattgcct
     8941 tctcaggtcg tatctggcaa aaaacggatt cacaaacttc taagtataaa aacagccgat
     9001 cgactgcaat ttttaacaaa agttacgaat tatatcatat ggatagggca aaaaaatctt
     9061 ctggaaaagc tagtgagatt tacttgatgg aaggattcat ggatgttatt gcagcctatc
     9121 gggctggaat cgaaagtgct gtggcgtcga tgggaacggc cttgagtcga gagcatgttg
     9181 agcatctgaa aaggttaacc aagaaattgg ttcttgttta cgatggagat aaggctgggc
     9241 aagccgcgac attgaaagca ttggatgaaa ttggtgatat gcctgtgcaa atcgtcagca
     9301 tgcctgataa cttggatcct gatgagtatc tacaaaaaaa tggtccagaa gacttggcct
     9361 atctattaac gaaaactcgt attagtccga ttgagttcta cattcatcag tacaaacctg
     9421 aaaacagtga aaatctgcag gctcagattg agtttcttga aaaaatagct cccttgattg
     9481 ttcaagaaaa gtccatcgct gctcaaaaca gctatattca tattttagct gacagtctgg
     9541 cgtcctttga ttatacccag attgagcaga ttgttaatga gagtcgtcag gtgcaaaggc
     9601 agaatcgcat ggaaggaatt tccagaccga cgccaatcac catgccaatc accaagcagt
     9661 tatcggctat tatgagggca gaagcccatc tactctatcg gatgatggaa tcccctcttg
     9721 ttttgaacga ttaccgtttg cgagaagact ttgcatttgc tacacctgaa tttcaggtct
     9781 tatatgactt gcttggccag tatggaaatc ttcctccaga agttttagca gagcagacag
     9841 aggaagttga aagagcttgg taccaagttt tagctcagga tttgcctgct gaaatatcgc
     9901 cgcaggaact tagtgaagta gagatgactc gaaacaaggc tctcttgaat caggacaata
     9961 tgagaatcaa aaagaaggtg caggaagcta gccatgtagg agatacagat acagccctag
    10021 aagaattgga acgtttaatt tcccaaaaga gaagaatgga gtaataatgg caacaaaaca
    10081 aaaagaagta acaacatttg acgtacaggt agcagaattt atccgtaatc ataagcaaaa
    10141 agggacagca acagatgatg aaatcaatgc aagtctggtt attcctttta ccttggacgc
    10201 tgatgggatt gaagatctct tgcaacggat tcaggatgca ggaatttcta tcacagataa
    10261 cgaaggaaat ccaagtgcgc gtgttcttag caatgaagaa gaaccagaac tcagcgatga
    10321 ggacttgatt gggtcaactt ctgctaaggt caatgaccct gtccgtatgt acttgaaaga
    10381 aataggggtc gttcctctct tgaccaatga agaggagaaa gagttggcac tggctgttga
    10441 agctggtgat atcgaagcca aacaacgtct tgcggaagcc aatcttcgtt tggttgtttc
    10501 cattgccaaa cgctatgtcg gtcgtggcat gcagttcctt gacttgattc aagaaggaaa
    10561 tatgggcttg atgaaggcgg ttgacaagtt tgactattct aaagggttca agttttcaac
    10621 ttatgcaact tggtggattc gtcaggctat cactcgtgct attgcggacc aagctcgtac
    10681 catccgtatc ccagttcaca tggttgaaac tatcaataaa ttggttcgtg aacagcggaa
    10741 tctccttcaa gaattggggc aagatccgac accagaacag attgctgaac gaatggatat
    10801 gacacctgat aaggttcgtg aaatcttgaa gattgcccaa gaaccagtat ctcttgaaac
    10861 tcctatcggt gaagaggacg atagccacct tggagacttt atcgaagatg aagtgattga
    10921 aaatccagtg gattatacga ctcgtatcgt cttgcgtgag caattggatg aaatcttaga
    10981 tactcttaca gaccgtgaag aaaatgttct gcgtctacgt tttggactag atgatggaaa
    11041 aatgcgcaca cttgaagatg tggggaaagt ctttaacgta actcgtgagc gtatccgtca
    11101 gattgaagca aaggctttga gaaaactacg ccaaccaagt cgtagcaaac cgcttcgtga
    11161 ttttattgaa gactaagagt gaggaaaata tggcttatac agaagagcaa attgaaaaca
    11221 tcaaaacacg gattttaaca gccttggaag aagtcatcga ccctgagttg ggaatcgata
    11281 ttgtcaatct tggtttgatc tatgagattc gttttgacgg tgacacaggg caaacagaga
    11341 ttgacatgac tttgacaact atgggttgtc ccttagcaga ccttttgaca gaccagattt
    11401 atgatgctat gatagaggtt ccagaagtaa cggatactga ggttaaatta gtctggtatc
    11461 cagcctggac agttgaaaaa atgagtcgct atgcccgcat tgccctaggc attaagtaaa
    11521 catataaaag catgtgagag actgttggaa aagcgaggaa atttcccctc ttttcctcta
    11581 gtctctcctt tcttttgctg attttattca aagaaaatga tataatagta gttatggaga
    11641 aaaagaaatt acgcatcaat atgttgagtt caagtgagaa agtagcagga cagggagttt
    11701 caggtgctta ccgtgaatta gttcgtcttc ttcaccgtgc tgccaaggac caattgattg
    11761 ttacagaaaa tcttccaatc gaggcagatg tgactcactt tcatacgatt gattttccct
    11821 attatttatc aaccttccaa aagaaacgct cagggagaaa gattggctat gtgcatttct
    11881 tgccagctac acttgaggga agtttgaaaa ttccattttt cttaaaggga attgtgaaac
    11941 gctatgtatt ttctttttac aaccggatgg agcacttggt tgtggtcaat cctatgttta
    12001 ttgaggattt ggtagcagct ggtattccac gtgaaaaagt gacctatatt cctaactttg
    12061 tcaacaagga aaaatggcat cctctaccac aagaagaggt agtcagactg cgcacagatc
    12121 ttggtcttag tgacaatcag tttatcgtag taggtgctgg gcaagttcag aaacgtaaag
    12181 ggattgatga ctttatccgt ctggctgagg aattgcctca gattaccttt atctgggctg
    12241 gtggcttctc ttttggtggt atgacagatg gttatgaaca ctataagaaa attatggaaa
    12301 atccccctaa aaatttgatt tttccaggca ttgtatcgcc agagcggatg cgcgaattgt
    12361 atgctctagc ggatcttttc ttgttgccta gttacaatga gctctttcct atgactattt
    12421 tagaagctgc gagttgtgag gctcctatta tgttgcgtga tttagatctc tataaggtaa
    12481 ttttggaggg aaattatcgg gcgacagcgg gtagagaaga gatgaaagag gctattttgg
    12541 aatatcaagc aaatcctgct gccttaaaag atctcaaaga aaaggctaag aatatttcca
    12601 gagagtattc tgaagagcat ctgttacaaa tctggttgga cttttatgag aaacaagccg
    12661 ctttagggag aaagtaaaaa gtgaggtaat ctatgcgaat tggtttattt acagatacct
    12721 attttcctca ggtttctggt gttgcgacca gtattcgaac cttgaaaaca gaacttgaaa
    12781 agcagggaca tgctgttttt atctttacga cgacagataa ggatgtcaat cgctacgaag
    12841 attggcaaat tatccgcatt ccaagtgttc ctttctttgc ttttaaggat cgtcgctttg
    12901 cctaccgagg ttttagcaag gcacttgaaa ttgctaaaca gtatcagcta gatattatcc
    12961 atactcagac agaattttct cttggcctgt tggggatttg gattgcgcgt gaattgaaaa
    13021 ttccagtcat ccatacctat cacacccagt atgaagacta tgtccattat attgctaagg
    13081 ggatgttgat ccggccgagt atggtcaagt atctggttag aggtttcctg catgatgtgg
    13141 atggggttat ttgtcctagt gagattgtcc gtgacttgct atctgattat aaggtcaagg
    13201 ttgaaaaacg ggtcattcct actgggattg aattagccaa gtttgagcgt ccggaaatca
    13261 agcaggaaaa tttgaaagaa ctgcgtagta aactagggat tcaagatggt gaaaagacgt
    13321 tgcttagtct ttcgagaatc tcctatgaaa aaaatattca agcagtttta gcagcctttg
    13381 ctgatgttct gaaagaggaa gacaaggtta aactggtagt agctggggat ggcccttatc
    13441 tgaatgacct caaagagcaa gctcagaacc tagagattca agactcagtc atctttacag
    13501 ggatgattgc tcctagtgag acggctcttt actataaagc ggcggatttc ttcatttcgg
    13561 catcgacaag cgaaacgcaa ggtttgacct acttggaaag cttagccagt ggaacacctg
    13621 tcattgctca cggaaatcct tatttgaaca acctcatcag tgataaaatg tttggaacct
    13681 tgtactatgg agaacatgat ttggctggtg ctattttgga agccctgatt gcaacaccag
    13741 acatgaacga gcatacctta tcagagaaat tgtatgagat ttcagctgag aactttggga
    13801 aacgagtgca tgagttttat ctggatgcca ttatttcaaa taacttccag aaagatttgg
    13861 ctaaagatga tacggtcagt cagcgtatct ttaagacagt tttgtatctt cagcaacagg
    13921 tggttgctgt acctgtaaaa ggatctagac gcatgttgaa ggcttcaaaa acacagttga
    13981 tcagtatgag agactattgg aaagaccatg aagaatagaa agaggaacag ctatgaaaaa
    14041 aacaattaat gagaagcggt cgtgataaaa agattgcggg tgtttgtgct ggggtggccc
    14101 attatctgga tatggatccg actatcgttc aagtcatttg gggtgttctt acttgctgtt
    14161 acggagctgg aattgtagct tacattattt tatggattat cgcaccagta gcaactgact
    14221 attgaaaact agctcaattc atgctaagat aatagaaaat agaatgtaac gaaggagaga
    14281 aaaatggcat ttggagataa tggaaatcgt aaaaaaacta tgtttgagaa aataaccttg
    14341 tttatcgtga ttatcatgct agtagcaagt ttattgggaa tttttgcaac tgcaattggt
    14401 gccctcagta atctataaaa tagattcaag aaaatttagt gactgggatt tcccagccct
    14461 tttttaaagt gagaagaaat aatgagtatg tttttagata cagctaagat taaggtcaag
    14521 gctggtaatg gtggcgatgg tatggttgcc tttcgtcgtg aaaaatatgt ccctaatgga
    14581 ggcccttggg gtggtgatgg tggtcgtgga ggcaatgtgg tcttcgttgt agacgaagga
    14641 ctacgtacct tgatggattt ccgctacaat cgtcatttca aggctgattc tggtgaaaaa
    14701 gggatgacca aagggatgca tggtcgtggt gctgaggacc ttagagttcg agtaccacaa
    14761 ggtacgactg ttcgtgatgc ggagactggc aaggttttaa cagatttgat tgaacatggg
    14821 caagaattta tcgttgccca cggtggtcgt ggtggacgtg gaaatattcg tttcgcgaca
    14881 ccaaaaaatc ctgcaccgga aatctctgaa aatggagaac caggtcagga acgtgagtta
    14941 caattggaac taaaaatctt ggcagatgtc ggtttagtag gattcccatc tgtagggaag
    15001 tcaacacttt taagtgttat tacctcagct aagcctaaaa ttggtgccta ccactttacc
    15061 actattgtac caaatttagg tatggttcgc acccaatcag gtgaatcctt tgcagtagcc
    15121 gacttgccag gtttgattga aggggctagt caaggtgttg gtttgggaac tcagttcctc
    15181 cgtcacatcg agcgtacacg tgttatcctt cacatcattg atatgtcagc tagcgaaggc
    15241 cgtgatccat atgaggatta cctagctatc aataaagagc tggagtctta caatcttcgc
    15301 ctcatggagc gtccacagat tattgtagct aataagatgg acatgcctga gagtcaggaa
    15361 aatcttgaag aatttaagaa aaaattgtct gaaaattatg atgaatttga agagttacca
    15421 gctatcttcc caatttctgg attgaccaag caaggtctgg caacactttt agatgctaca
    15481 gctgaattgt tagacaagac accagaattt ttgctctacg acgagtccga tatggaagaa
    15541 gaagcttact atggatttga cgaagaagaa aaagcctttg aaattagtcg tgatgacgat
    15601 gcgacatggg tactttctgg tgaaaaactc atgaaactct ttaatatgac caactttgat
    15661 cgtgatgaat ctgtcatgaa atttgcccgt cagcttcgtg gtatgggggt tgatgaagcc
    15721 cttcgtgcgc gtggagctaa agatggggat ttggtccgca ttggtaaatt tgagtttgaa
    15781 tttgtagact aggagactgg tatgggagat aaaccgatat ctttccgaga tgcggatggt
    15841 aattttgttt ccgccgcaga cgtttggaat gaaaagaaat tggaagaact atttaatcgt
    15901 ctcaatccaa atcgtgcctt gagattggca cgaactaaaa aggaaaatcc atctcagtaa
    15961 agaagctaaa aaatcccgtg cctcatcaga cacgggattt tgtggtacga caggcatgta
    16021 tagcaaactg aatctggaat agcacagcat atcttctaaa atattgctat aaattgattt
    16081 gaatttcaaa attaaattgt tttattctta tttcaatttg ttatagtata tctgatgtca
    16141 aagttctcgg cgagtcaaat agtgattccc aagcctgact atcgtgaggt agcggattaa
    16201 aatggtctgg ggatagaccg ttttaagtct gatgctggaa ataagaattg tcagaagaag
    16261 ggatagcgaa atcgtggctc tacgaacagg aacgtgataa taaggcgtat atagcgaata
    16321 agagggcctc aaactctaaa gtccaaaaag gtagtcgtaa cctatatgcg taaatcatga
    16381 gagtaattga attcgtacta agattttcta tttttactgt aaccttttaa cgtcctcata
    16441 tcttgtatac acgaggaaag atgtacgact tatcccgtga ggtctatcac tataaagaga
    16501 aaacgacaga tagaagtgat cctgagtcac ggttatcttt aaaatagatt gtctgatagg
    16561 atgctagcct gaaaacgtct tgttcatgaa atataggacg gtatgtataa aacgcttctg
    16621 tgaactgaga gaagggggag aagttcttgc taaaatttag ttgaacagcc gtattccgat
    16681 acttagataa gagatctagt cttagctcct actcagtttc aggggataaa aaaggggcaa
    16741 tagcgattcg agaaagatta tactcttcga aaatctcttc aaatcacgtc aatatcgcct
    16801 tgccgtatgt gtaggatact aactacgtca gttccatcta caatctcaaa acagtgtttt
    16861 gagcaacctg cggctagttt cctagtttga tctttgattt ttattgagta ttagtaattc
    16921 agttactaac tcgtcaactc tgatttatcc aataaaattg aaaaggatgg aaaaaaggat
    16981 aaatttatga tatactttat tttgaagacc ttattagaaa tcttgaaaga gtattgaaaa
    17041 cttagaatga gaaaaattgt tatcaatggt ggattaccac tgcaaggtga aattactatt
    17101 agtggtgcta aaaatagtgt tgtggcctta attccagcta ttatattggc tgatgatgtg
    17161 gtgactttgg attgtgttcc agatatttcg gatgtagcca gtcttgtcga aatcatggaa
    17221 ttaatgggag ctactgttaa gcgttatgac gatgtcttgg agattgatcc aagaggtgtt
    17281 caaaatattc caatgcctta tggtaaaatt aacagtcttc gtgcatctta ctatttttat
    17341 gggagcctct taggccgttt tggtgaagcg acagttggtc taccgggagg atgtgatctt
    17401 ggtcctcgtc cgattgactt acaccttaag gcgtttgaag ctatgggtgc cactgctagc
    17461 tacgagggag ataacatgaa gttatctgct aaagatacag gacttcatgg tgcaagtatt
    17521 tacatggata cggttagtgt gggagcaacg attaatacga tgattgctgc ggttaaagca
    17581 aatggtcgta ctattattga aaatgcagcc cgtgaacctg agattattga tgtagctact
    17641 ctcttgaata atatgggtgc ccatatccgt ggggcaggaa ctaatatcat cattattgat
    17701 ggtgttgaaa gattacatgg gacacgtcat caggtgattc cagaccgcat tgaagctgga
    17761 acatatatat ctttagctgc tgcagttggt aaaggaattc gtataaataa tgttctttac
    17821 gaacacctgg aagggtttat tgctaagttg gaagaaatgg gagtgagaat gactgtatct
    17881 gaagacagca tttttgtcga ggaacagtct aatttgaaag caatcaatat taagacagct
    17941 ccttacccag gctttgcaac tgatttgcaa caaccgctta cccctctttt actaagagcg
    18001 aatggtcgtg gtacaattgt cgatacgatt tacgaaaaac gtgtaaatca tgtttttgaa
    18061 ctagcaaaga tggatgcgga tatttcgaca acaaatggtc atattttgta cacgggtgga
    18121 cgtgatttac gtggggccag tgttaaagcg accgacttaa gagctggggc tgcactagtc
    18181 attgctgggc ttatggctga aggtaaaact gaaattacca atatcgagtt tatcttacgt
    18241 ggttattctg atattatcga aaaattacgt aatttaggag cggatattag acttgttgag
    18301 gattaaaccg tagaggtgtt tatgaatatt tggaccaaat tagcaatgtt ttcttttttt
    18361 gaaacggatc gcttgtattt gcgtcctttc ttttttagtg atagtcagga cttccgcgag
    18421 atagcttcaa atccagaaaa tcttcaattt attttcccaa cgcaggcaag tctggaagaa
    18481 agtcaatatg cactggccaa ttactttatg aagtcccctt tgggagtgtg ggcaatttgt
    18541 gaccagaaaa atcaacaaat gattggttct attaaatttg agaagttaga tgaaatcaaa
    18601 aaagaagctg agcttggcta ttttttgaga aaagatgctt ggtcgcaagg atttatgaca
    18661 gaggttgtta gaaaaatttg tcagctttct tttgaggaat ttggcttaaa acaattattt
    18721 atcattaccc accttgaaaa tgaagctagc caaagagttg ctcttaagtc tggatttagt
    18781 ttgttccgtc agtttaaggg aagtgatcgt tacacaagaa aaatgcggga ttatcttgaa
    18841 tttcggtatg taaaaggaga gttcaatgag taagcatcag gaaattctaa gctatttgga
    18901 ggaattacca gtaggtaaaa gggtcagtgt tcgtagcatt tcgaatcatc taggagttag
    18961 tgatggaaca gcctatcgag ctattaaaga agctgaaaac cgtggaattg tggagacccg
    19021 tcctagaagt ggaacaattc gtgttaaatc ccagaaagtt gctatagaga gattaacgtt
    19081 tgctgaaatt gcagaagtga cttcttctga ggttctggct gggcaagaag gtttagagag
    19141 agagtttagt aagttttcaa ttggtgccat gactgaacaa aatatcttgt cttaccttca
    19201 tgatgggggg ctcttgattg tcggagaccg aacccgtatt cagttgctag ccttggaaaa
    19261 tgaaaatgca gttctggtta cagggggatt tcaggttcat gatgatgtgc ttaaactggc
    19321 caatcaaaaa gggattcctg ttctaagaag taagcatgat acctttaccg tcgcgaccat
    19381 gatcaataaa gccttgtcaa atgtccaaat caagactgat attctgacag ttgagaaact
    19441 ttatcgccct agtcatgagt atggttttct gagagagaca gatacagtta aagattattt
    19501 ggacttggtt cgtaagaatc gtagcagccg tttccctgtt atcaatcaac atcaggtcgt
    19561 tgttggtgtt gtaaccatga gagacgctgg tgataaatca ccaagcacga caattgataa
    19621 ggttatgtct cgtagtctat tttgggttgg attatcgaca aatattgcca atgtgagtca
    19681 acggatgatc gcagaagact ttgaaatggt accagttgtt cgaagcaatc aaactttgct
    19741 tggcgttgtg acgcgacgag atgtcatgga gaagatgagc cgttcccaag tttcggctct
    19801 accaactttt tctgagcaga ttggacaaaa gctctcttat caccatgatg aagtagtcat
    19861 tacagtggaa ccctttatgc tagaaaaaaa tggagttttg gctaatggtg tattggcaga
    19921 aattctgacc cacatgaccc aagatttagt tgttaatagt ggtcgcaatc tcattatcga
    19981 gcagatgctg atctactttt tgcaggctgt tcagatagat gatatattgc gcattcaggc
    20041 acggattatt catcatacga gacggtcagc tataattgat tacgatattt atcatggtca
    20101 ccagattgtt tcaaaagcaa atgtgactgt taaaattaat tagaaactag gagaaaagat
    20161 gataacatta aaatcagctc gtgaaatcga agctatggac aaggctggtg attttctagc
    20221 aagtattcat ataggcttac gtgatttgat taagccaggc gtagatatgt gggaagttga
    20281 agaatatgtc cgccgtcgtt gtaaagaaga aaatttcctt ccacttcaga ttggggttga
    20341 cggtgccatg atggactatc cttatgctac ctgttgctct cttaacgatg aagtggctca
    20401 cgctttccct cgtcattata tcttgaaaga tggtgatttg ctcaaagttg atatggtttt
    20461 gggaggtccc attgctaaat ccgacctgaa tgtctcaaaa ttaaacttca acaatgttga
    20521 acaaatgaaa aaatacactc agagctattc tggtggttta gcagactcat gttgggctta
    20581 tgctgttggt acaccgtccg aagaagtcaa aaacttgatg gatgtaacca aagaagctat
    20641 gtacaagggt attgagcaag ctgttgttgg aaatcgtatc ggtgatatcg gtgcggctat
    20701 tcaagaatac gctgaaagtc gtggttacgg tgtagtgcgt gatttggttg gtcatggtgt
    20761 tggcccaact atgcacgaag aaccaatggt tcctaactat ggtattgcag gtcgtggact
    20821 ccgtcttcgt gaaggaatgg tcttaaccat tgaaccaatg atcaatacag gcgattggga
    20881 aattgataca gatatgaaaa ctggttgggc gcataagacc attgacggtg gattgtcatg
    20941 tcagtatgaa caccaatttg tcattacgaa agatggacct gttatcttga ctagccaagg
    21001 tgaagaagga acttattaat aaaaagtgaa aagactactg gaagtttatt ttgataaaaa
    21061 atccagtaga tcttttcata ataaaacgca ttgtatcaag tgttaggagc tgatatcatg
    21121 cgtttttctg cttttaagat tttttccaac tctgtttgta agcgcatcat aacaaagggt
    21181 ctaggattca gggctctcct cctatatact attagtaaag taaaactaag agaggatatt
    21241 ttagtgtcgc agtctattgt tcctgtagag attccacaat attgtcgttt tgattctaaa
    21301 aagagaaatg gaattctgtt taacgttcgt attgccaatc ttaaatttac cttttttaga
    21361 ttagacttcc tgcgaaa
//
LOCUS       DAVLSL010000021        21028 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLSL010000021 DAVLSL010000000
VERSION     DAVLSL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.021"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..6360
                     /locus_tag="VXN30_000621"
     CDS             <1..6360
                     /locus_tag="VXN30_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1357286.1"
                     /translation="SHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGVAASVQTV
                     QAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEVTKP
                     AEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAGQSL
                     LGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAKPAA
                     TTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKEDVNRGYS
                     GFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRSGNS
                     VNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPRVREQSKSISQGANYV
                     SHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSNDPYS
                     NSNYYYRANPLYLDTNPYGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQR
                     QITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNN
                     LTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDQGERGLTGAQGEKGAQGQAGRD
                     GVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRNDADKTTTVI
                     FYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKGEDGTHTIT
                     INDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTVIFYYDLNDNNQFDEGDTKLKE
                     VVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHTITITQPDNRPSLTTIVKNGED
                     GKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDELVQTTVVKDGQDGAAGASGRD
                     GKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTWEPAGNIKGPKGDKGADGAKGE
                     KGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQGERGLTGAQGAKGDKGEQGLQ
                     GRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDGKSLIAVKTGTETKVYVEDPAR
                     PGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNGKNGVLVTITPVGGRPQTTFVE
                     DGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTTTIRDGATGQAGRDGKDVLNGK
                     VNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIKGPKGDKGADGAKGEKGDQGER
                     GLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQGKDGDKYVNTETGDVFVKNNGN
                     WDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHSTDITFTVPGKDPVTVNVKDGEN
                     GLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKYTPGTDELLGSEMIKDGAKGA
                     DGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLAVIRDGVDGKSPTVTAVRKDE
                     AGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQDGQTPTITTQRGQDGQSTVVTI
                     TTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRDTDAAATPTRNGIRVTVYD
                     DVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTKDNGDGTHTITVQNPDGSEST
                     TVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVVKNGKDGKTPKVEVTDNND
                     GTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSHTVTITNPDGTKNEFDVKN
                     GRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRDGREPKLEVIDNNDGSHTI
                     KVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGRGYKSIIKDGKDGKDSV
                     SPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINS
                     DGTVTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEV
                     PVKPVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNAN
                     PVAPTTGKENRGDKLPETGSQSDYISVLSGSGILLSLYVGRRKEA"
     gene            complement(6597..7145)
                     /locus_tag="VXN30_000622"
     CDS             complement(6597..7145)
                     /locus_tag="VXN30_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1357287.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            7466..7954
                     /locus_tag="VXN30_000623"
     CDS             7466..7954
                     /locus_tag="VXN30_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1357288.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            7951..9138
                     /locus_tag="VXN30_000624"
     CDS             7951..9138
                     /locus_tag="VXN30_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1357289.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQVAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            9131..9496
                     /locus_tag="VXN30_000625"
     CDS             9131..9496
                     /locus_tag="VXN30_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1357290.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            9512..10858
                     /gene="asnS"
                     /locus_tag="VXN30_000626"
     CDS             9512..10858
                     /gene="asnS"
                     /locus_tag="VXN30_000626"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1357291.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            11011..11301
                     /locus_tag="VXN30_000627"
     CDS             11011..11301
                     /locus_tag="VXN30_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1357292.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            11313..11783
                     /gene="ssbA"
                     /locus_tag="VXN30_000628"
     CDS             11313..11783
                     /gene="ssbA"
                     /locus_tag="VXN30_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1357293.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            11815..12054
                     /locus_tag="VXN30_000629"
     CDS             11815..12054
                     /locus_tag="VXN30_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1357294.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            12349..13749
                     /locus_tag="VXN30_000630"
     CDS             12349..13749
                     /locus_tag="VXN30_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1357295.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            13751..14110
                     /locus_tag="VXN30_000631"
     CDS             13751..14110
                     /locus_tag="VXN30_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1357296.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            14164..14913
                     /locus_tag="VXN30_000632"
     CDS             14164..14913
                     /locus_tag="VXN30_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1357297.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKRVKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            14903..15202
                     /locus_tag="VXN30_000633"
     CDS             14903..15202
                     /locus_tag="VXN30_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1357298.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            15273..16208
                     /locus_tag="VXN30_000634"
     CDS             15273..16208
                     /locus_tag="VXN30_000634"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1357299.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            16294..16806
                     /locus_tag="VXN30_000635"
     CDS             16294..16806
                     /locus_tag="VXN30_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1357300.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERVCRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            16831..17439
                     /locus_tag="VXN30_000636"
     CDS             16831..17439
                     /locus_tag="VXN30_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1357301.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDRKYELAIDFDKERLTFYLA"
     gene            17540..17734
                     /locus_tag="VXN30_000637"
     CDS             17540..17734
                     /locus_tag="VXN30_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1357302.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(17780..19225)
                     /locus_tag="VXN30_000638"
     CDS             complement(17780..19225)
                     /locus_tag="VXN30_000638"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1357303.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            19331..20953
                     /locus_tag="VXN30_000639"
     CDS             19331..20953
                     /locus_tag="VXN30_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1357304.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
BASE COUNT         6636 a         3939 c         4768 g         5685 t
ORIGIN      
        1 tcacataaaa aatcatttga ctggtatagc atgcaacaac gttattctat tcgtaagtat
       61 tactttggtg cagctagtgt cttgctcggt accgctttgg tattaggtgt agcagctagt
      121 gtccaaacag tacaagcgga agaaaacaaa caagaaacta ccaatagtat ttctgttggt
      181 aggggagaag cagctactaa accagcagag gtttctgcgt ctaataaaga gaaaacctat
      241 gcagctccaa ctgtagctaa tccagtagaa acgactccag ttaaaactga agaagttact
      301 aaaccagcag aaaaagttga agaagcaaaa gacaaaaaag aggaagtaac gcatcaagat
      361 gccgttgaca agtcaaaatt attaacggct ctttcgcgtg ctaaaaaatt agaaagcaag
      421 ttatatacag aagcaagtgc tgctaacttg caaacaagta tccaagctgg tcaaagcttg
      481 cttggaaaag cagatgcaac tgaagctgaa ttatcagcag cagagtcatc tattcaatca
      541 tttattattg gtctagaact tcgttctaac tctaataaag aaactgtatc agaaacgcct
      601 gtagcgaaga aagctgatgc agttgaatca aaagaagggg ctaaaccagc tgcaacaact
      661 gaacgttcag ctgttgatag cgctattttg ccaactagca cagctgacaa agtagaaaca
      721 acttcagctc cagcatctat taatgaaatc ttgaaactag gtttgagcct ttctgatgct
      781 cgccaaaatc cagctatccg taaggaagat gttaatagag ggtatagtgg ttttagagcg
      841 gctagcaatc cagccaaccc aattgtctca ggttctggaa atacagttgc atttgcagat
      901 attagccaag gtggtcgtag ctatagtttc cgtggctacg ggaactcacg tggtggaaat
      961 tctattcatt acgatgtaac aacagtacgt agtggtaata gtgttaactt tacaattagt
     1021 tattctgcgc caggagattc tagagagttt gttaataata attttatctt ggataaaggg
     1081 gatggatttg gaaatccttc aaatgcaacg atcacaagtt caaatccaag agtaagggag
     1141 caatcaaaat ctattagtca gggtgccaac tacgtatctc actcaggata cagtatgacc
     1201 tctgctattt caacaaatac ggaacaaact atcagattta gtttgcctat cattaatcta
     1261 aatggtgatt tgtctgttcg tttgaaacct gttactttta acgtggacca aggtggtggt
     1321 ggtgccgcta ctagcaatga cccatacagt aactctaact attattatag agcaaaccca
     1381 ctatacttgg atacaaaccc ttatggtggt actaataata agactgtttc agaagacatt
     1441 gacttccaaa ctgtctatct tccaactagt aagttaccag aaggtcaaac tagattagtt
     1501 cgagaaggtg aaaaaggaca acgtcaaatt acctataaag ttcatcgatt tggtaacgaa
     1561 acacttttag gattgccgat tagtaatagt gttactaaag aagctaagcc acgtattatg
     1621 caaattggtg tggctaaaga tctaatcgat acagtaaaac cacgtgttga tcaaaataaa
     1681 gtcggtgata caaataacct cactttctat cttgataacg atggaaacgg tgtttatact
     1741 gaaggtgtag acgaacttgt tcaaaaaatt gctattaaag atggagctaa aggtgaaaaa
     1801 ggagaccaag gtgaacgcgg tctcactgga gcccaaggtg aaaaaggagc ccaaggtcag
     1861 gcaggtcgtg acggtgtaac tccaaccgta accgttaaag ataataaaaa tgacggcact
     1921 catactatca ctattaacga cggtagaggt aatgttacaa gtactgttgt aagagatggt
     1981 ttcgatggcg caagtccatt agttgcgact caacgaaatg acgcagataa gacaacaact
     2041 gttatcttct attacgataa aaatggaaac aatgaattag atgcttctga taagaaatta
     2101 aaagaagttg taattgcaga tggtgctaaa ggtgaaaaag gggacaaagg tgaacaaggt
     2161 cttcaagggc gtgatggtga acaaggacca aaaggagaag atggaacgca tacaattaca
     2221 attaacgatg gtaaaggtgg tataactact acagtagtaa gagatggatt tgatggtgca
     2281 agccctcttg tttctactca tagaaatgaa gcagataaaa caacaactgt tattttctat
     2341 tatgatctaa atgataataa tcaatttgat gaaggagata caaaacttaa agaagttgtt
     2401 atcgcagatg gaaaacaagg accaaaaggt gacaaaggtg ataacggaaa agatggattc
     2461 acaccagaag taacagttac agataacaat aacggaacac acaccatcac aatcacacaa
     2521 ccagacaaca gaccatcatt aacaacaatc gttaaaaacg gtgaagatgg aaaaacacca
     2581 aaagtcaaag cagaacgaga tgatgcgaag aaacaaacaa cattaacatt ctacattgac
     2641 aaagatggag atggaagtta cacagcagga aaagacgagt tagttcaaac aacagtagtt
     2701 aaagacggac aagacggagc tgcaggagca tctggacgtg atggaaaaga agtattaaac
     2761 ggaaaagtag acccaacaac agaaggtaaa gatggagaca cattcgtaaa tacacaaaca
     2821 ggagatgtat tcgttaagaa aggtaacact tgggaaccag caggaaacat caaaggaccg
     2881 aaaggtgaca aaggtgcaga tggtgccaag ggtgaaaaag gagcccaagg agaacgcggc
     2941 ctgactggag cgcaaggtgt caagggtgaa aagggagatc agggtgaacg cggtctaact
     3001 ggatctaaag gtgaaaaagg agaccaaggt gaacgcggtt taactggagc gcaaggtgcc
     3061 aaaggtgaca aaggtgaaca aggtcttcaa ggtcgtgatg gagctcaagg accaaaaggc
     3121 gcagatggac aaagaggacc agcgggacca caaggaccaa aaggagaaca aggtaatcca
     3181 ggaactccag gtaaagatgg aaaatctcta attgctgtta aaactggaac tgaaacaaaa
     3241 gtctatgtag aggatccggc tagaccagga caaccactta atccaaacca accattagca
     3301 actatcactg atggaagaaa cggtactaat ggacaatcac caacaattac agcgacacga
     3361 agtgtgcaaa atggcaaaaa tggtgtatta gtaacaatta caccagtagg aggtcgtcca
     3421 caaacaacat ttgtagagga tggacaaaag ggtgctgatg ggaaaacccc aacagtaaca
     3481 ataactgagg ggcaaaacgg cacacataca ttaacggttc ataatccagg aagtccagat
     3541 gtgacaacta cgatccgtga tggagctaca ggacaagcag gtcgtgatgg taaagatgta
     3601 ttaaacggaa aagtaaatcc acaaccaaac caaggtaaaa atggagataa atatattaat
     3661 accgaaaccg gtgatgtcta tgttaaaaac aatggaaact gggataaaga aggcaacatc
     3721 aaaggcccta aaggggacaa aggtgcagat ggtgctaaag gcgaaaaagg agaccaaggc
     3781 gaacgcggcc taactggagc gcaaggagct aaaggtgcgg atggcgcagt aggtcgtgat
     3841 ggacgtgacg gtaaagacgt gttgaacggc aaagctaacc cagaagcaca tcaaggtaaa
     3901 gatggcgata aatacgttaa tacagaaaca ggcgacgtct tcgttaagaa taacggcaac
     3961 tgggataaag agggcaacat caaaggccct aagggtgaca aaggtgaacg cggagaagat
     4021 ggtaagactc cagaagtaac tgtaactcca ggtaaagatg gccatagtac tgacattaca
     4081 ttcactgttc caggtaaaga tccagttaca gtaaatgtta aggacggaga aaatggtctg
     4141 aacggtaaaa ctccaaaagt tgatttactt cgtgtccaag gaaaaaacgg aaatccatct
     4201 catacaattg tgacattcta tacagatgaa aacaatgacg gcaaatatac accaggaact
     4261 gatgaacttc taggttcaga aatgattaaa gatggtgcta aaggcgcgga cggacgagat
     4321 ggtaaatcat tgcttactgt caaggatggt aaagaaacta aagtttacca agaagatcca
     4381 gctaacccag gacaaccatt aaatccagaa aaaccacttg cggtaattag agatggagta
     4441 gatggaaaat cacctacagt tacagctgtt cgtaaagatg aagcagggca taaaggtgta
     4501 gaaatcactg ttgataacca tgatggttca caaccaacta cagtctttgt tcaagatggt
     4561 gctaagggag aaactggtgc aaccggtcag gatggacaaa ctcctacaat cactactcaa
     4621 cgtggacaag atggccaaag cactgttgta actatcacaa catcaggtaa agatccagta
     4681 accttcactg taaaagatgg taagaatggt aaagatggcc gtgcaccgaa aatcaaagta
     4741 gaagatatta cttcaccttc aagaattaga cgcgatacag atgctgctgc aactccaacg
     4801 cgtaacggta tccgtgttac agtttatgat gatgttaatg acaatggggt atacgacgaa
     4861 ggtgtcgata aagtattaaa tagtaaagat atttataacg gtatagatgg acgtgatggt
     4921 tcagctccaa ctattactac aaaagataat ggagatggaa ctcacactat cacagttcaa
     4981 aacccagatg gttctgaatc aacaacagtt gttaaagatg gtaaagacgg taaaactgcg
     5041 aatatcacta caacagaaaa cccagatgga agccacacaa ttacggtgac aaatccagat
     5101 ggttcaacta aagaaactgt tgttaaaaac ggtaaagacg gtaagactcc taaagttgaa
     5161 gtaacggata acaacgatgg aactcatact gttaaagtta cagatggaga cggcaatgtt
     5221 accaacgcta tcatcaaaga tggtaaagac ggtaaagctg caacagcaac aactactgaa
     5281 aatccagatg gaagccacac agtaacaatc actaacccag acggaactaa gaatgagttt
     5341 gatgttaaga atggacgtga cggtgttgac ggacgtactc caaccgcatc tgttcgtgat
     5401 aatggagacg gaagtcatac aatcgttatt acaaatccag aaggtgtgac aactgaaacc
     5461 acagttcgtg atggtagaga accaaaactt gaagttattg ataacaacga tggttcacac
     5521 actattaaag tgacaggtgc tgatggtaaa ggaacgacaa ctacaatctt tgatggtaaa
     5581 tcaccaaaag cgaacatcgt tgataacgga gatggaactc atacattaac aatcgtagat
     5641 tctgatggtc gtggatacaa atctattatc aaagatggta aagacggcaa agatagcgtt
     5701 tcaccaactg taactgttaa aaataataac gatggaactc acgttgttac aatcactaat
     5761 ccagatggaa gtaagacaga aatggtgatt aaagacggta aagatggtaa atcaccaaaa
     5821 gtttctgttg aagataatgg tgatggtagt catacaatca caatcatcaa ttctgatgga
     5881 actgtgacaa aaacagttat taaagatggt agagatggac gtgatggtcg agacggcaaa
     5941 gacggtaaag acggtaaaga tggaaaatgt ggatgccaag acaaaccagt aacaccatca
     6001 aatgacaaac cagttcctcc aacaccaaat gtgccgacac cagaagtacc ggttaaacct
     6061 gtgccagcgc aaccaacacc aaatgtaccg acaccagaag tgccagtaca accaactcca
     6121 gctgttccaa caccagaagt accggttaaa ccagtaccag cggttccaga acaaccagta
     6181 gtaccaacac cggctcaacc agcaactcca gtaaatgcta acccagtagc accaactaca
     6241 ggtaaagaaa accgtgggga caaattacct gaaactggaa gccaatctga ttatatctct
     6301 gttctttcag gtagcggtat tctattgagc ctatatgtag gacgaagaaa agaagcttaa
     6361 tcagccaaaa gcagtcacta atttctcgaa agaagatgat aagtgataga tctcgaatga
     6421 aagaatattc ttagaatgat tttcctatga tggaaaagac tgctaaaaac cttgagtggt
     6481 aacgctcaag gtttttttgg atggctgatg tttgtctgaa aatcttgatt gagaaataaa
     6541 aagccaccct attaggtggc tataattgat ttagactttc taaaattttc tattttttac
     6601 tgaatcgctt cgaccaagtc ttgagcttgt ttttcaagtg agtttaggac tgtttcttca
     6661 agaaccaatt ttccgtctgc ccaggcagag tcattaacac gtgcagcagt gaaatcacca
     6721 acgccttgtg tacggataaa tggcaagagg tctttgtaga tagcgaaaag ttgatcgtgc
     6781 cctgcattgg ctacagatga tactgtgaca aacttgtctt gaagggcaga aacgccacgt
     6841 gtatcagaca agtcaagggc acgagatagc cagtcaagca agtttttcac tgtaccaggg
     6901 atagagaagt tgtagactgg agagaaaatc cagatagcat ccgcaacgag aactgcttca
     6961 cgagcagcag ctacagctgg atgtgttgga acttccaaat cttggctgaa gagaggaagg
     7021 gctgaataat caaggtagct aacttccgct ttaccagcaa gtgctttctc agcttcgagc
     7081 gccatttggt ggttgaaaga accttgacgt agtgatccga cgataaataa tactttttta
     7141 gacatgatgt gtctcctttt tcttaaaatt tcgagaactc tctcgttgtt ataaaatata
     7201 ttacaacaaa acaaaagaac ttgcaagaaa tttgctcaga gtttatttga aaattgtaaa
     7261 atttcggaaa ctctagtcat ttgtgactga aaaagtaaga actgtttcac tttcaaaatc
     7321 ggttttgttt gaagggagaa atagaattgg ttgctcaaag cacagctttg aggttgcaga
     7381 taaagctgac gtggtttgaa gagattttcg aagagtattc ttatattcac ttgtaataaa
     7441 gcttaataga tggtaaaata gacgagtgaa aaaaagacaa aacaaagcaa aaaataatct
     7501 actgtggcag tatggtctag ggatgacgat tttgtttgtg gttatcagtg cttcctttct
     7561 gtatatggtt tctcttagca tgaaacccta tcaaacagct aaaagtgaag gagaaaaatt
     7621 agctcagcag tatgcaggat tagagcaggc tgatcaggtt gatttataca atggcttgga
     7681 atcttattac agcgttcttg gtcgtaacaa acagcaagaa gcgcttgctg tcctgattgg
     7741 taaagatgac cataagattt acgtttatca gctaaatcag ggtgtttcac aagaaaaagc
     7801 agaaacggtt tctaaggaaa agggagctgg cgagattgac aagataacct ttggccgtta
     7861 tcaagataag ccaatctggg aagttaagtc aggatctgat ttttatctag tagattttga
     7921 aacaggagca ttggtcaaca aggagggcct atgaaactat ccaaccgtgt tttagaaatg
     7981 gaagaaagtg tgactttggc tgctggagcc cgtgccaaag cactgaaggc tgagggtaga
     8041 gatattttgt ctctaacctt gggtgagcca gatttcacta ctcccaaaaa tatccaagtt
     8101 gccgccattg catcgattcg agatgggcgt gcttcttttt atacagtaac ctcaggtctg
     8161 ccagagctta aggcggcggt caatagctac tttgagcgct tttacggcta ttctgtagcg
     8221 tcaaatcaag tgacagtcgc tgcgggagcc aaatattctc tctatacctt ctttatggct
     8281 gtggtcaatc caggtgatga agtcatcatc ccaaccccat actgggttag ctatggagat
     8341 caggtcaaga tggcagaagg cgttcccgtc tttgtttctg ctaaggaaga caatcacttt
     8401 aaggtgaccg tagagcagtt agaagcagct cgcactgaca agaccaaggt tttggtgctg
     8461 aattcgccat ctaatcccac aggtatgatt tacactcgtg aggaactctt ggcaattgga
     8521 aactgggctg tagaaaatga tattctcatc ctagcagacg atatctatgg ccgcttggtt
     8581 tataatggtc atgagttcac accgatttct agcctatcgg aagcgattcg caagcaaaca
     8641 gtggtcatca atggtgtgtc taaaacttat gccatgactg gttggcggat tggttatgcc
     8701 gttggagaag cagacattat cgctgccatg tccaagattg caggtcaaac aacttcgaat
     8761 ccgtcagcag tagcccaata tgcagcagtt gaggctctat caggtgagca agatactgta
     8821 gaaagcatgc gtcaggcctt tgaggaacgt cttaatacca tctatcccct ccttgcagag
     8881 gtaccaggat ttgaagtggt caaaccgcaa ggggccttct atctcttccc aaatgtcaaa
     8941 aaggccatgg agatgaaagg ctacacggat gtgacagact ttacaactgt tatcttagaa
     9001 gaagccgaag tggccttggt aacaggagct ggatttggag caccagaaaa tgtccgcctc
     9061 agctatgcga cagacctaga cacgcttaaa gaagcagtcg aacgcttgaa agcatttatg
     9121 ggtagtgaga atgattgatc attttgagat taaggtaaag gatttacaaa tttcagaagg
     9181 attttatagg agttttctcg ctcctttgga ctataaattg gcttttaaga ctagttctct
     9241 aattagtttt ctatccccga acagccctca tcctggtggt gatttttggc tgactcaggg
     9301 aacacaagat cctgttcatt ttgctttttt agcagaaaat aaggaagaag ttcaggcttg
     9361 ttatgaggct ggcttagagg caggtgggcg agacaatggg gctcctggtt atcgaagtga
     9421 gcatccgatt tactatgctg cttttatgat tgacctggat gggaacaata tagaggtggt
     9481 ttgccataaa gaataaaaaa gaaaaggaaa aataatgaca aaacgtgtaa cgattattga
     9541 cgtaaaagac tatgttggtc aggaagtgac gattggcgct tgggttgcca acaaatcagg
     9601 aaaagggaaa atcgctttct tacaattgcg tgatggaaca gccttctttc aaggtgtggc
     9661 ttttaaacca aactttgtcg aaaaatttgg tgaagaagtg ggacttgaga agtttgatgt
     9721 tatcaaacgc ttgagccaag aaacgtctgt ttatgtgaca ggtattgtca aagaggacga
     9781 acgttctaaa tttggctatg agttggacat cacagacatc gaagtgatcg gtgaatctca
     9841 agactaccca atcacaccaa aagaacacgg aacagacttt ttgatggata accgtcactt
     9901 gtggctacgc tctcgtaagc aagtagctgt gttgcaaatc cgtaacgcta ttatctatgc
     9961 aacttatgag ttctttgaca agaacggttt tatgaagttt gacagcccaa ttctttcagg
    10021 aaatgcggca gaagattcta cagaactctt tgaaactgac tacttcggaa cgccagccta
    10081 cttgagccaa tcaggtcaac tttacctaga agcaggggct atggctcttg gtcgtgtctt
    10141 tgactttggt ccagttttcc gtgctgaaaa atcaaaaaca cgccgtcact tgactgagtt
    10201 ctggatgatg gatgctgagt actcatactt gacacatgat gagtcgcttg acttgcaaga
    10261 agcttatgtg aaagctcttc tacaaggtgt tcttgaccgc gcgcctcaag ccttggaaac
    10321 cttggaacgt gatacagaac tcttgaaacg ctacattgca gagccattca aacgtatcac
    10381 ttacgatcaa gccattgacc tcttgcaaga gcatgaaaat gatgaagatg ctgactacga
    10441 gcatcttgag catggtgatg actttgggtc accacacgaa acttggattt caaaccactt
    10501 tggtgtgcca acatttgtca tgaactatcc agcagccatc aaggccttct acatgaaacc
    10561 agttcctgga aatccagagc gcgtgctttg tgcagacttg cttgctccag aaggctatgg
    10621 agaaattatc ggtgggtcta tgcgtgagga agattacgat gcccttgtcg ctaagatgga
    10681 tgaacttggc atggatcgta cagaatatga attctacctt gaccttcgta aatacggtac
    10741 agttccacac ggaggatttg gtatcggtat cgaacgtatg gtaaccttcg cagcaggaac
    10801 aaaacatatc cgtgaagcta ttccattccc acgtatgttg caccgtatca aaccataaga
    10861 acaggaaaac gcccatgtgg cgttttttcg aattctagtg tttttacttg cgtaaaaaaa
    10921 ttttttctag tataatagtt tattgtgagc gaacctcact taccccttgc aaagtcttgg
    10981 ggtcattaga ccaaaaggag gaacatatca atggctaaat acgaaattct ttatatcatt
    11041 cgtccaaaca ttgaagaaga agctaaaaac gctttggtag cacgttttga ctctattttg
    11101 actgacaacg gtgcaactgt tgttgaatca aaaacttggg aaaaacgtcg tcttgcatac
    11161 gaaatccaag atttccgtga aggactttac cacatcgtta acgttgaagc aaatgacgat
    11221 gcagctctta aagagtttga ccgtctttca aaaatcaacg ctgacattct tcgtcacatg
    11281 atcgtcaaaa ttgacgcgta agaaggtaaa ctatgattaa caatgttgta cttgtagggc
    11341 gtatgacacg tgacgctgaa ttgcgttata ccccatcaaa tgtagcagtt gcgactttta
    11401 ctcttgcagt aaaccgtaca tttaagagtc aaaatggtga acgtgaggct gattttatca
    11461 atgtcgttat gtggcgccaa caggctgaaa atcttgctaa ctgggctaaa aaaggctcac
    11521 ttatcggggt gacaggtcgt atccagactc gtagttacga taaccagcaa ggacaacgtg
    11581 tctacgtgac agaggtcgtg gctgagaatt tccaaatgtt ggaaagccgt agtgtgcgtg
    11641 agggtcacac aggtggagct tactctgcac caactgcaaa ctattcagca cctacaaatt
    11701 cagtaccaga cttttcacgt aatgaaaatc catttggagc aacaaaccca ttggatattt
    11761 cagatgatga tttaccattc taatggacaa ctaaacttat aaaggagaaa aaacatggct
    11821 caacaacgtc gtggcggatt caaacgccgt aaaaaagttg attacatcgc agcaaacaaa
    11881 attgaatatg ttgattacaa agatactgag cttcttagcc gtttcgtttc agaacgtggg
    11941 aaaatccttc ctcgtcgtgt aacaggaact tcagctaaaa accaacgtaa agtaacaaca
    12001 gctatcaaac gcgctcgcgt aatggctttg atgcctttcg taaacgaaga ttaaagaaaa
    12061 gacccagttg ggtctctttt tcatgagctt gaaaacaaga aagcgaatta agaccccaac
    12121 gggtcttttt tcacaagctt tgaaatgaga gagcgagtta ggctcagtgg ctcccttttt
    12181 acgagtttga aaacaagaga acacattaaa agcacgatgg agctttttca taaatatgtc
    12241 tcttttctca tccaagaaat taaaataaaa atgataaact ttttgaaaat aggggatttg
    12301 cctactttat gatataatgg aaagagttag attgaaagag gtagtgagat ggacgcgaaa
    12361 ttaagataca aggcaaagaa gatcaagatt gtcttttttg atattgatga tacattgcgg
    12421 aattcaaaga cagggtttat tccaactaca attcccactg tttttaaaca gttgcgtgaa
    12481 aaaggaattt taacaggaat cgcctctgga cgtggcattt ttggtgttgt tccagagatt
    12541 cgtgatctca agcctgactt ttttgtaact ttgaatgggg cttatatcga agataaaaaa
    12601 ggtcaggtca tttatcagca tcagattgaa aagtcagatg ttgaggagta tatctcttgg
    12661 gctaagcaag aaggaattga gtatggcttg gttgggagtc atgatgccaa gttgtcgact
    12721 cgcaccgata tgatgagtga agctatcaat ccaatttatc ccgacttaga tgtagatccc
    12781 gatttccatg aaaaagaaga tatctatcag atgtggactt ttgaagataa gggagatgac
    12841 ttgcacttgc ctgacagtct ctcagacaaa cttcgcatgg ttcgttggca tcaacattcg
    12901 tctgatattg tgccgatttc aggctccaaa gcgacggggg tggaaaaggt tgtggaacac
    12961 cttggcttga aaccagagaa ggtcatggtt tttggagatg gtctcaacga cttggaactc
    13021 tttgattatg ctggaatcag cgttgccatg ggaatttctc atgataaaat caaagaaaaa
    13081 gcagattata ttacaaaaac attagaagaa gatggcattt ttgctgcctt agaagtattt
    13141 ggtatggtag aaaaagaatt gcattttcca caagtagaca ttgaaacagt agaaggtcct
    13201 cttgcgacta ttaagaccaa tcacggagac ttacgtatca agcttttccc tgaacatgct
    13261 cctaaaacag tggctaactt tgtatctctt tcaaaagatg gctactatga tggtgtcatt
    13321 ttccaccgta ttatcaagga ctttatgatc caaggtggag acccaactgg aactggtatg
    13381 ggtggccagt caatctacgg cgaatctttt gaggatgaat tctcagaaga gctttacaat
    13441 atccgtggtg ctctttccat ggcaaatgct ggtccaaata ccaacggcag ccagttcttt
    13501 atcgtgcaaa accaacacct accttattct aagaaagaaa ttactcgtgg tggttggcca
    13561 gaaccgattg cagaaatcta tgccaatcaa ggtgggacac ctcacctaga ccgccgtcac
    13621 acggtttttg gtcagttagc tgatgaagca tcttacgctg tcttggatgc cattgctgct
    13681 gttgagacag gagctatgga caagccagtt gaagatgttg taattgaaac tattgaaatc
    13741 gaggactaag atgaaaatcg gtgataagct aaaggggcgt attacaggga ttcagcccta
    13801 cggtgccttt gttgagttag agacgggtga tacggggctg attcacattt cagaaattcg
    13861 gacaggattt attgaaaata ttcatgaaac cttgaaagtc gatgaagagg ttcaagttca
    13921 ggtagtggat ttagatgaat ttacggggaa agccagtctt tctatccgca ctttggagga
    13981 agaaaagtac cagtttccta gacggagacg cttttcaagc gaccgcttta actacggttt
    14041 tgctcccttt cgacgaatgc taccaatctg gactggagaa gccctgcacc atttaaaaaa
    14101 gaagaagtag tttagagttt tatatttttt gtaatgttaa tatgaatttg ctataatagg
    14161 accatggaag aagaacaatt attaaaatca ggagagcgca ttaaccagct cttttcgaca
    14221 gatatcaaaa tcattcaaaa tagagaggtt tttagctatt cggtggatag tgttctctta
    14281 tcacgatttc cacgttttcc taagaagggg ttgattgtgg atttctgtgc tgggaatgga
    14341 gcagtggggc tttttgctag cactcgtact caagcacaga tattgtctgt tgagattcag
    14401 gagcgtttgg cggatatggc tgaacgctct gtccgtttga atggattaga ggagcaaatg
    14461 caggtcatct gcgatgattt gaaaaatatg cctgctcaca ttcaggggag taaggtggat
    14521 atgattttgt gtaatccacc ctatttcaag gtggatcctt attctaatct gaacgagagt
    14581 gaacattatc tcttggctcg acacgaaatc acgactaatt tggaagaaat ctgtcgtagt
    14641 gcccagagta ttctcaagtc taatgggcgt ttggccatgg ttcatcgtcc tgatcgactt
    14701 ctggatattt tggatacgtt aaaacgacat aatctagccc ctaagcgcct gcagtttgtt
    14761 tatccaaaaa gagtaaagga agccaatatg cttttgattg aggcgatcaa ggatggctca
    14821 acaagtggct ttaaggtctt acctcctttg attgtccaca atgatgatgg ctcttatacg
    14881 cccgaaatcg aagagattta ttatggatca taaggcttat atgtacgttc tggagtgtcg
    14941 tgatggatcc tactatatag gctatacgac tgatatgaga agacgcctcg ctatccacaa
    15001 tagtgggaag ggagccaaat atacacgagc acgcttgcca gtcaaactta tctatgctca
    15061 aggttttgcc agtaaggaag aagccatgtc ggctgaagct cttctcaagc gtaagaagag
    15121 gccacagaag gaagaatttt tatctgaaaa tcaagataga aatttactca gttattttga
    15181 agaatcgtgg ggagtccttt gactcctctt acctttgtca aaaagcgaaa aaaatgctac
    15241 aataaagaaa ataaacaaaa tgaggtatta tcatgtccaa gattctagta tttggtcacc
    15301 aaaatccaga ctcagatgcc atcggatcat ctgtagcttt tgcctacctt gcaaaagaag
    15361 cttacggttt ggatacggaa gctgttgccc ttggaactcc aaatgaagaa acagcctttg
    15421 tcttgaacta ttttggtgtg gaagcaccac gtgttatcac ttctgccaaa gcagaggggg
    15481 cagagcaagt tatcttgact gaccacaatg aattccaaca atctgtatca gatatcgctg
    15541 aagtagaagt ttacggtgtt gtagaccacc accgtgtggc taactttgaa actgcaagcc
    15601 cactttacat gcgtttggag ccagttggat cagcgtcttc aatcgtttac cgtatgttca
    15661 aagaacatgg tgtagctgtg cctaaagaga ttgcaggttt gatgctttca ggtttgattt
    15721 cagataccct tcttttgaaa tcaccaacaa cacacccaac agataaaatc attgctcctg
    15781 aattggctga attggctggt gtgaacttgg aagaatatgg tttggcaatg ttgaaagctg
    15841 gtaccaactt ggctagcaaa tctgctgaag aattgattga tatcgatgct aagacttttg
    15901 aactcaacgg aaataatgtc cgtgttgccc aagtgaacac agttgacatc gctgaagttt
    15961 tggaacgcca agcagaaatt gaagctgcaa tgcaagctgc caacgaatca aacggctact
    16021 ctgactttgt cttgatgatt acagatatcg tcaactcaaa ctcagaaatc ttggctcttg
    16081 gtgccaatat ggacaaggtc gaagcggctt tcaacttcaa acttgaaaac aatcatgcct
    16141 tccttgctgg tgccgtttca cgtaagaaac aagtggtacc tcaattgact gaaagcttta
    16201 atgcgtaaga ttttgggtgt cagctcaaaa tcggaaagtc tagtttgcct tatatcgaaa
    16261 ggagtttcgg ctcctttttt ctaggagtga agtatgttag aaaatggcga tttgattttt
    16321 gtgagagatg ggtcagacat gggacaggcc atccagactt ccacaggtaa ctatagccat
    16381 gttgccattt atttggatgg gatgatttat catgctagtg gacaggctgg tgttgtctgt
    16441 caagaaccgg cagacttctt tgagtccaat catttatacg acctctatgt ttacccagaa
    16501 atggatatcc agtcggtgaa ggaaagagtt tgcagacatc ttggagcacc ctacaatgct
    16561 tctttctatc cagatgcagc tggtttttac tgctcccagt atatagcaga aatcctacct
    16621 atttttgaaa ctattcctat gaaatttgga gatggggagc aggagattag tgatttttgg
    16681 agggagtatt acatagaact aggtctgcct gttcctctga accaagctgg taccaatcct
    16741 agtcagttgg cagcatcgcc tctgttacaa tgtaaagaaa ggaatcttca tgattcagat
    16801 ttttaatcca tctcgtttga cgagacagcc atttttggag aattgatccg ctatctggac
    16861 cagtatgagg atgtgattct acgggaaatt aaggctcaat ttccagatgt tgcagttgat
    16921 aaactcatgg aagagtatat aaaggcaggc ttgattctac gtgaaaataa gcgctattac
    16981 ctcaattttc ctacgcttga atcacttgat agtcttgaac tggatcaaga gatttttgtc
    17041 agagaagcta gtccggtcta tcaagccttg ttggagcaga gttttgagac ggaattgcgc
    17101 aatcaaatca atgcagctat tttagttgaa aagacggact ttgcgcgcat taaaatgacc
    17161 ctgtccaatt atttttacaa ggtcaaacag cagtatcctt tgacagaaaa acagcaggag
    17221 ctctatgaca ttttaggaga tgttaatcct gagtatgcac tcaagtatat gacggctttt
    17281 ttgttgaaat ttctcaaaaa agaccagctt atgcagaaat gccgtgatat ctttgtggac
    17341 agtttggttg tcttaggcta tattgtgcaa aatgaagata gaaagtatga gttggctatc
    17401 gattttgata aggagagatt aactttctac ttagcgtgat ttcttgtttc tgagcacatt
    17461 gtttgacttt ccttagtatt cggtataaac tatatgtaac cggtaacaca tatcggaata
    17521 aactaaagga gacaatcata tgtcacttga aaacaaattg gaacaagcaa caggcgctgt
    17581 caaagaaggt tttggtaaag ttactggaga cagcaagaca gaacttgaag gagctgttga
    17641 aaaaacagtt gctaaggcaa aagacgttgt agaagacgca aaaggtgctg tagaaggtgc
    17701 cgttgaaggt ttgaaaaacg tttttactaa ataataggaa aaaatcaagg gtttcatttt
    17761 cccttgattt tttctattct tataaataat tttctgcgac ggctgtatct cctgggtagg
    17821 attctttctt gccctggatg atttggtaac aatcggctcc cttacccgca ataataactg
    17881 catctaattc gtgatttgtg atagccattg ccgccttgat ggcttcttgg cgatccgcaa
    17941 tcttttcaac aggatgattg atgtagctac taatttcatc tgcaatggcc attgggtctt
    18001 catagttagg gtcatcagca gtcagaaaga cttgaatctc agggtgttga ttgaggagga
    18061 ggccaaagtc cttacgacga ctttctccct tgtttcctgt tgatcccaga accagagcaa
    18121 tctttccggt ttgatgagtt tcaaccacat tgatgagttt tttcagacta tccccattgt
    18181 gggcatagtc gatgaagacc ttggctccat ttttctgagt gaggacttcc atacgaccag
    18241 gaacgcgggt tgcagcgatg ccttttttga tgtcctcaag acttgctccg agacggagac
    18301 aagcaagtcc agcagcaact gcattttctt ggttgaagtt gccaatgagt tggatatcat
    18361 aatctccagc gagtttaccc gtagctgaaa agctaaaggc tttggaattc tcgatttggt
    18421 tatcaaattg gctaccatag aaatcatggt cttgatcttc aacctgttct ttcaagactg
    18481 agaagtggtc catgtcactg ttaatgatga ctgctcggct attttccatc aagagacgct
    18541 tgtggtagaa atagtcttca aagctagggt gttcaatcgg gccgatatgg tcaggagtga
    18601 tgttaagaaa gactcctaca tcaaaggtca gtccatagac tcgatggact agataggctt
    18661 gactggagac ttccatgatg aggtgggtac ggtcattttg cacagcctga ttcatcatgt
    18721 caaagaggtc aatactctca ggggttgtca acgctgactt aaagaaagtc tcgccatcaa
    18781 gagttgtgtt catggtcgac aacatagcag gtctatgccc ttgagataag atgttatagg
    18841 cgaaataggt tgctgttgtc ttacccttag taccagtaaa ggcaaggagt ttgagtttct
    18901 cttgtggatt accatagaac tccatggcaa tcaaactcat ggctttcttt atatcgttca
    18961 caatgatgac agggatatcg acttcgtagt ccttttcagc tacataccaa gctaaacctt
    19021 gtgttatagc agaaagaagg tattcttttt taaaggcagc gccttttgca aaaaaaagag
    19081 tgtcttctgt tacttttcgg ctgtcgtagc tgatgctatc aaaaataact ttgctgtagt
    19141 tgtagtggta atgaccttgg tcaataattt cgcgaaaaag gccatctttc tttaaaatat
    19201 ctaatacggt ttcaatctta atcatacttt ctattgtaaa ccgaaagtcg taaatttaca
    19261 agtaacaagg aaaagtttat aatggaagat aaggagtttt tcctagttat caaaattgaa
    19321 tgaggaatct atgtcgcacg aaaacaatca ccagcaggcc cagatgttac gggggactgc
    19381 ttggctaacg gctagtaact ttatcagtcg cctactcggg gctgtttaca ttatcccttg
    19441 gtacatctgg atgggggctt atgcagctaa ggcaaatggt ctctttacca tgggttacaa
    19501 tatctatgct tggttcttgt tggtttcaac agcggggatt ccagttgcgg tggccaagca
    19561 agttgccaag tataatacca tgcgagaaga agagcatagc tttgccctga ttcggagctt
    19621 cttaggcttt atgacaggac taggcctggt ttttgcttta gtcttgtatg tctttgctcc
    19681 ttggctagca gacttgtctg gcgtgggcaa agacttgatc ccaatcatgc aaagcttggc
    19741 ttggggagtc ttgattttcc cgtctatgag tgttatccga ggatttttcc aagggatgaa
    19801 taacctcaaa ccctatgcta tgagccaaat tgctgagcag gtcattcgtg ttatctggat
    19861 gctcctagca acctttatca ttatgaagct cggttcagga gattatctag cagccgttac
    19921 ccaatcaacc tttgctgcct ttgtcggtat ggtagccagt tttgcagtct tgatttattt
    19981 ccttgcccaa gaaggttcac tcaaaagagt ctttgaaaca ggagataaga ttaacagtaa
    20041 gcgtctcttg gttgatacca ttaaggaagc cattcctttt atcctgacag ggtctgccat
    20101 ccagctcttc cagattttgg atcagctgac ctttatcaat agtatgagct ggtttaccaa
    20161 ctacagcaat gaggacttgg ttgtcatgtt ttcttatttc tcagccaatc ctaataaaat
    20221 cacgatgatt ttgatttctg taggggtttc gattgggagt gttggtttgc cacttttgac
    20281 ggaaaactat gtcaaggggg acttgaaagc agcttctcgt ctcgttcagg acagtctcac
    20341 cctactcttt atgttcttgc taccagcaac ggttggagtg gttatggtag gagaacctct
    20401 ttatacggtc ttctatggta agccagatag tttggctctg ggcttatttg tctttgcagt
    20461 tttgcagtct attattttag gcttgtacat ggtcttgtct ccaatgcttc aggccatgtt
    20521 ccgcaaccgc aaggccgttc tctattttat ctatggttct attgccaagc tagtcttgca
    20581 actacctacc atcgccctct tccacagtta tggtcctttg atttcaacaa ccattgctct
    20641 catcattcct aacgtcttga tgtatcggga tatttgtaaa gtaactggtg tcaagcgcaa
    20701 ggtgattttg aagcgaacca ttttaatcag tttgctgacc ctagtcatgt ttctgttaat
    20761 aggaaccatc cagtggctgt taggattttt cttccaacca agtggacgtt tgtggagctt
    20821 cttttatgta gctcttgtcg gtgccatggg gggtggactt tatatggtta tgagtctgcg
    20881 tacctattta ttagataagg taataggaaa agcccaagca gatcgcctgc gagcaaaatt
    20941 taagctttcg taaggtagca tttataaata aaaggaataa gttgaacaat cttttaggag
    21001 aacttgaaac ttattccttt tttagcgt
//
LOCUS       DAVLSL010000022        19967 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLSL010000022 DAVLSL010000000
VERSION     DAVLSL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19967
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.022"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(13..648)
                     /locus_tag="VXN30_000640"
     CDS             complement(13..648)
                     /locus_tag="VXN30_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1357305.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(650..1477)
                     /locus_tag="VXN30_000641"
     CDS             complement(650..1477)
                     /locus_tag="VXN30_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1357306.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMFEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1499..1834)
                     /locus_tag="VXN30_000642"
     CDS             complement(1499..1834)
                     /locus_tag="VXN30_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV1357307.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(1835..2366)
                     /locus_tag="VXN30_000643"
                     /pseudo
     CDS             complement(1835..2366)
                     /locus_tag="VXN30_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            2997..3608
                     /locus_tag="VXN30_000644"
     CDS             2997..3608
                     /locus_tag="VXN30_000644"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1357308.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3653..4381
                     /gene="rlmB"
                     /locus_tag="VXN30_000645"
     CDS             3653..4381
                     /gene="rlmB"
                     /locus_tag="VXN30_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1357309.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(4420..5331)
                     /gene="trxB"
                     /locus_tag="VXN30_000646"
     CDS             complement(4420..5331)
                     /gene="trxB"
                     /locus_tag="VXN30_000646"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1357310.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5397..5621)
                     /locus_tag="VXN30_000647"
     CDS             complement(5397..5621)
                     /locus_tag="VXN30_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1357311.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5699..6442)
                     /locus_tag="VXN30_000648"
     CDS             complement(5699..6442)
                     /locus_tag="VXN30_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1357312.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6442..7242)
                     /locus_tag="VXN30_000649"
     CDS             complement(6442..7242)
                     /locus_tag="VXN30_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1357313.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7400..7756)
                     /locus_tag="VXN30_000650"
     CDS             complement(7400..7756)
                     /locus_tag="VXN30_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1357314.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7769..8281)
                     /locus_tag="VXN30_000651"
     CDS             complement(7769..8281)
                     /locus_tag="VXN30_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1357315.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(8281..8775)
                     /locus_tag="VXN30_000652"
     CDS             complement(8281..8775)
                     /locus_tag="VXN30_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357316.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            8910..9356
                     /locus_tag="VXN30_000653"
     CDS             8910..9356
                     /locus_tag="VXN30_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1357317.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            9353..10000
                     /locus_tag="VXN30_000654"
     CDS             9353..10000
                     /locus_tag="VXN30_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1357318.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(10021..10602)
                     /gene="pdxT"
                     /locus_tag="VXN30_000655"
     CDS             complement(10021..10602)
                     /gene="pdxT"
                     /locus_tag="VXN30_000655"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1357319.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(10603..11478)
                     /gene="pdxS"
                     /locus_tag="VXN30_000656"
     CDS             complement(10603..11478)
                     /gene="pdxS"
                     /locus_tag="VXN30_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1357320.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(11630..13009)
                     /locus_tag="VXN30_000657"
     CDS             complement(11630..13009)
                     /locus_tag="VXN30_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1357321.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            13303..14226
                     /locus_tag="VXN30_000658"
     CDS             13303..14226
                     /locus_tag="VXN30_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1357322.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14286..14891
                     /locus_tag="VXN30_000659"
     CDS             14286..14891
                     /locus_tag="VXN30_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1357323.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFSDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            14910..16154
                     /locus_tag="VXN30_000660"
     CDS             14910..16154
                     /locus_tag="VXN30_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1357324.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(16377..16634)
                     /locus_tag="VXN30_000661"
     CDS             complement(16377..16634)
                     /locus_tag="VXN30_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1357325.1"
                     /translation="MDPKKIARINELAKKKKTEGLTSEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(16676..18712)
                     /locus_tag="VXN30_000662"
     CDS             complement(16676..18712)
                     /locus_tag="VXN30_000662"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1357326.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(18924..19841)
                     /gene="glyQ"
                     /locus_tag="VXN30_000663"
     CDS             complement(18924..19841)
                     /gene="glyQ"
                     /locus_tag="VXN30_000663"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1357327.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
BASE COUNT         5803 a         4548 c         3623 g         5993 t
ORIGIN      
        1 ttatttagct atttagtaaa gatagtcttt caaggctttt gacaaagcct gataaccagc
       61 aatactgagg tgcagtccat cagttgtata ttcttttttg agttggcctg cttggtctgt
      121 caaacaatca aatactggca caaattccac ctgcatatag gcagatgcaa gctcttgata
      181 ggcttgattc cagttctgaa ttttttcatt cgagcggata tagactgcct gctggtactc
      241 ctctccctca ttgacaggca aaatggaaag caatttaatc tctgtcaatg gataatcgcg
      301 agcaacggat tgaatgatag cttcgagatt attgagagcc tcattcacag gaacatcctt
      361 tccgatatca tttgtcccaa tcagaagaaa aattttatct actgctccac catatagatg
      421 agcatcaagg ttctctagta acagtcctgt ctgatagcca cgaattcctc gattgacaat
      481 cgtctttgaa gtcccaaata gctcctgtag aggataatac tcgacaatgg aatccccaat
      541 aaaaagaatg tttggttcta caacagaaat gtgatttagg tgacgatact tagtttgaat
      601 tttttcttgt tcctttagga gccaattttc taataactgt actgccactt cattctcctt
      661 tttctaacca gttttccaag acttgataaa ctcctccttg gctgttggct ggtgctagag
      721 cagtcgccac agctttggct ttctcatcag cattttccat cgcataggca attccagcca
      781 tttcaaacat ttcaacatca ttttcactat caccaaaagc catgatttct tgggatttca
      841 agtcccagcg cttgagtaat tcctccaagc cccatgcttt atgaatccca gcttggagga
      901 tatcaatgca accatagcca ctggatacag ctcggacacg gccatcaaag agagcattga
      961 tttcttccaa aaccgaactc aaacgttcct caccaacaac catgctcatc ttgagcacac
     1021 caccaaagag gtcagatgtt aattcatcca caaattgcat ccgttggtag aatttttcaa
     1081 tcatttctgg agtcataaaa ctttcaagat ctgtaaaaat cgtaccttcc ttgacaaaat
     1141 cacccttcat ccccgttaca acaaactggt cctgacacgc tcgacccttg aaatgagtca
     1201 aagccttgtt gacaatggcg tcatcccatg tctgagcctg aatcaattca ttgttttcaa
     1261 aaatacgagc gccattagca acaaccagaa ccactcgatc caccaaggga ctcagtagtt
     1321 gtctcatgcg gtgaatttca ttgcccgtcg caatgacaaa acgaatgccc ctttgatcca
     1381 actgatctaa aatcttttcc aatcgtggga gatcaagctg acctctagca tccagcaagg
     1441 tcccatccat atctgtcgca attaccttaa tcatcattta ctttcctctg gattcaagct
     1501 aataccactt caatcccaca tgttcattca tttctttata ggctgtatca attcgttcct
     1561 tagtcgcttc atctaactgg tcacgatgac tgccatcctc tataaaatct tccaaaatat
     1621 aagtttggta atgatacaag ggatatcctt ctggtgaaaa ggttcccttt ggtgttcgat
     1681 tgacccaagt aggatgagct ttagctgttc cgatagttgt ttttccatcc ttcttcttga
     1741 tggtgacatc catgagaaca ccacgttcag tccacttagc attctcttca tctcccatag
     1801 attcaatccg ttgattggaa atgaagttcc ccatttaata gacaatgagc ttcttatctc
     1861 catctttttc caccgtttca gatggttcaa caacgtgagg atgccctcca aagataatat
     1921 ccgctcccca atcgatcatc ttgtgataaa gagctttttg ttcttcagtt ggttccaatc
     1981 gatactcaac acccatctga agcatgataa tggtgatatc tgcttccttc tctgcccgtt
     2041 caatttcaac cttcatctta tcttcgttta aatctgaaag ataacgatta tagtcttcct
     2101 gagaaatata ctgctcaatt ccattgaaac cataggaata ggctaacaat gcaaccttga
     2161 taccattcac ttccttaatg accagcggag cctgaacacg tggttcgtgc gtataaactc
     2221 cgattggagt gattccagct ttctcaataa tatcggccgt tgaaataact ccctcaagtt
     2281 gaaataactc cctcaagttg cgaatccaaa atatgattat gagctaaatc cagcacatga
     2341 taacctgcct ccttaatagc atccataact tcagcaggag cattaaagag aagataaccc
     2401 gctaaataat gatccttatt aatggttcct tcaaaatcac caatagctaa atctgcttgc
     2461 ttgagccaag gagtcacata ctcaaaattt tcatgaaagt cataggtacc gtcttctttt
     2521 ttagctgaaa agaaaagtcc atcgtggtag agcaaatccc cgtgggccat aattctggca
     2581 gttttttcct cttcctactc ctgagacttt tgcttagtcc cctcttgaga aatagtatct
     2641 cgtttttgac tagtcaaggg attccttgga agctttcaaa caacaagacc aagcccttca
     2701 taaaacgaaa agctaaacca ccctgatttc gattttatct tctttgtatc ttcattctcc
     2761 ctactttctt atgcaagcct tttcttttta ttatatcaca gataagtatt tctttcacaa
     2821 ttgaattgaa cttcccatct attttctata aatcctaaat gccataatgc tttcaattct
     2881 tgtcattttg tgatatcatg tagaggaaat gaactaatcc acagtggctt atttcaagta
     2941 taccacttgg gctttggcag tagctaactg cactaaatat aatataagga gagaaaatgt
     3001 ctgcaataga acgtattaca aaagctgctc acttaattga tatgaacgat attatccgtg
     3061 aagggaatcc tactctacgc gcgattgctg aggaagtcac tttcccccta tctgaccagg
     3121 aaatcatcct aggcgaaaag atgatgcaat tccttaaaca ttcccaagac cctgtcatgg
     3181 ctgaaaaaat gggactccgc ggtggtgttg gactggctgc tccccagtta gatatctcaa
     3241 aacgcattat cgctgttttg gtacctaata ttgttgaaga aggcgaaact ccacaggaag
     3301 cctacgattt ggaagccatt atgtacaatc caaaaatcgt ctctcactct gttcaagatg
     3361 ctgctcttgg cgaaggagaa ggttgcctgt ctgttgaccg taacgtgcct ggctatgttg
     3421 ttcgccatgc ccgcgttact gttgactact ttgacaaaga tggagaaaaa caccgtatca
     3481 aactcaaagg ctacaactcc attgttgttc agcatgaaat tgaccacatt aacggtatca
     3541 tgttttacga tcgcatcaat gaaaaagacc catttgcagt taaagatggt ttactgattc
     3601 ttgaataaag aaaatcccgt tgcaagacgg ggttttgtgt tataatagag gcatgaaaac
     3661 aaatgatatt gtctatggcg tccacgccgt taccgaagcc ctccttgcaa atacaggaaa
     3721 caaactctac ctccaagaag atctccgagg taagaatgtt gagaaagtca aggaactagc
     3781 tacagaaaag aaggtgtcca tttcttggac atcaaaaaaa tctctctctg agattactga
     3841 aggtgctgtt catcaaggtt ttgttctacg agtgtctgaa tttgcctata gcgagctaga
     3901 ttacatcctt gcaaaaacac gccaagaaga aaatccactt ctattgattc tagatggtct
     3961 aaccgatccc cataatctgg gttctatctt gcgaacagcc gatgcgacca atgtttcagg
     4021 tgtcatcatt cccaagcacc gtgctgtcgg agtaactcct gtcgttgcca aaacagccac
     4081 aggtgctatt gaacacgttc caattgcccg agtgacaaac ctcagtcaaa ccttagataa
     4141 actcaaggat gaaggcttct ggacctttgg aacggatatg aacggtactc cttgccacaa
     4201 gtggaataca aaagggaaaa tcgccctcat catcggaaat gaaggaaaag gcatctctag
     4261 caacatcaaa aaacaggtcg atgaaatgat taccattccg atgaatggac atgttcaaag
     4321 cctcaatgcc agtgttgctg cggccattct catgtacgaa gtttttctaa atagactata
     4381 aaaaagtttc cagtcatctg attggaaact tttttatgat taactatgtt ctgtgataaa
     4441 cttgtaggct tcttgaccag cgatagctcc atctccaact gctgttgtta cttggcgaag
     4501 gtctttcaag cgaacatctc caactgcaaa gataccgtcg actgcagtct tcatgtggct
     4561 atctgtcaca atccagcctg cctgatcttg gatattcaat tctttaacaa aatcgctaag
     4621 agggtccaaa ccaacataga taaagacacc accgaaggct tgctctgtca cttgacctgt
     4681 tttcacattt tcaaaaacga cagattctac tctgttttca cccttgattt ccttcactac
     4741 agaatcccag ataaagctga ttttttcatt cgcaaaggcg cgatcttgta aaaccttttg
     4801 ggcacgaagt tggtcacgac ggtgaacaat ggtaacagtc ttagcaaaac gagtcaagaa
     4861 gagggcttct tcaacagctg aatctccacc accaactacc aataaatctt ggtcacggaa
     4921 gaaagcacca tcacacacag cacagtaaga aacaccacga ctgttcagtt cttcttctcc
     4981 aggcactccc aaaggacggt gtttagaacc agttgctacg ataactgtac gtgtttcata
     5041 tgtttggtca tcagtcatca ctttcttaaa atcaccatgg tcttcgacat tttcaacata
     5101 accataaatg tgctcaacac caagattttc aagtggttca aacatctttt cagccaattc
     5161 aggtccacta atattagcgt atcctgggta attttcgata tcagatgtat tattcatctg
     5221 accacctggc agaccacctt caatcaaagc tacttttaga ttgcttcgag cagcatacaa
     5281 ggccgcagtc atccctgcag gtccagcacc gataataata gtatcgtaca tatagattcc
     5341 ttctttcttg ttgtaactat ctttattcta actctttctt gtcaatcacg caaggattat
     5401 gccttgaaaa caagaattaa actcataacc gcaaaggata atactgggac aaccaagact
     5461 ccaatcataa tcatatcata gagatgggct tggcgagccc tggctgtctt atcaactccc
     5521 gacatggctc tcagaccaat ccaaatccct aaaaaaatca gaacaaggat ggtggtcaag
     5581 atcaaactct cgaaatataa agaaaatagt tgcagtagca tgatttctct catttctatc
     5641 ttttttaaag agtaaactca gctagtccaa ctaactgagt tttcctttat ctattatatc
     5701 aaatataagt ccgtttgtaa ctagcgaaga attcttttgt ccgctcttct ttaggggtgt
     5761 ggataatctc atccggagtt ccagactcga tgattttccc cttatctaag aagagaattt
     5821 tatccgcaac ttgggctaca aaggacatgt catgactgac caaaatcatg gtctgacctg
     5881 acttagcagc atctgcaata gacttttcta cttcaccgac caattctggg tcaagggctg
     5941 aagttggttc gtctaagagc aaaacatctg gtttcatagc aagcgcacgc gctagggcaa
     6001 cccgttgctt ctgtccacct gataaatggc gaggataatg gttttcacgg tccgaaagcc
     6061 caaccttagc caactcttcc ttggcaatct tagtcgcttc ttggtcagat aatttcttga
     6121 caacaaccaa gccttctttc acattatcaa gtgctgttcg acgttcaaat aaattaaact
     6181 gttggaaaac catagacaac ttacggcgta gggcaaggat ttcttcttga gtgattttag
     6241 aaaaatcaac tgaaaaacca tcaatctgaa tagagccact gtcaggtgtt tcaagataat
     6301 tgagactgcg aagaaaggtt gattttccag ctcctgaaga accaatcaag gctacaactt
     6361 cccctttttg aatatccaag ttcagatgat ccaagacagt ctgtcctgaa aaggatttgc
     6421 ttaaattcga aatcttaatc attaacgaag gtctcctttc acatctgttt gcactgtatc
     6481 aggtgcagaa atagccattt ttctctcgat gaaacgaccg aggctttcaa ttccgatatt
     6541 gactacccaa taaacaaggg caacagagat gaagcgttca aaatagcgat aatcagctcc
     6601 acctagaatc tgagcttggg caaagacttc cacaacaccc gcactaaaag ctagagatgt
     6661 tcccttggtc aaaccgatga gggaattaat caaggttgga gtagctacca ccgctgcatt
     6721 aggaataatc actcgtcgat aaacttgcgc tcgggtcata cccagactgc gcgccgcctc
     6781 aatctcacca ggattaactg agagaatggc tgcacgaatg gtttcactag cataagctgc
     6841 ctcattaaag gcaaaagcga caatcgcaaa agctgcagct ggaatcgcat tgatattgag
     6901 accagttccc cattgctgat tgagggcttt caaagccaaa ggaattccgt agtaggtcaa
     6961 catgagttgc accaaaatcg gtgtcccttt taagaaacta acaaagaagg cctgcaaggg
     7021 atataaaatc ttgacacgat tgatcttcac aatggcaaaa agaagcgcca aaaccaagcc
     7081 aaaaagggca ccaccaattg tcaacataat tgttgttgga agttgttgga caattctagg
     7141 gattccatca aagaccgaac gtaggctaaa cagcttgcca tccggaatca attgcatcaa
     7201 gttttggtac caatctgatg ctaaaatcgt tgtaacattc ataaaaacat cctcttctga
     7261 taatgattca ttctaccatg cttctgccgt caatgaaatt atttgctacg gtcagataca
     7321 gactttgtct acctactagt ttatcatact ttgaaacagg gaagttatat attttatcta
     7381 tcaaagagat agataaaaac tatttcagtc ccagttcttc ataagatttt cgataaccga
     7441 tttgcttaac agttccattt tctactaaaa tgggccgttt taacaacata ccgtcacttg
     7501 ctagcaactc agccgcttct tggtttgaca aacttcctac cttatctttt agccctaatt
     7561 cacggtattt gataccactg gtgttgaaaa attgcttcaa ttcaaatcct gaggtttcta
     7621 gccaattcaa aatgacttct tggctaggtg tttcttccac gatatggacg gctttatagt
     7681 ccacacctaa ttgatttaat tcttgttttg cttttttaca agttgaacat ttggggtatt
     7741 cgataaattc taacatgtgc ttctccttct atttgtttat atctttaaaa tctacgcctt
     7801 catgctccaa gagccaagct ttcttttcca ctcctgcagc atagcctgtc agacgcttgc
     7861 ctgctcccaa cacacgatga caaggtacta ggatagacca aggattgcgt cccactgctc
     7921 caccaattgc ttgagcagaa gccacttgca ggtcttgagc aatttgtcca taggtcactg
     7981 tctgaccata aggaatgccc tgtaaatagt cccaaactct cttttcaaaa ttcgttccga
     8041 ttggcgccaa gagcaagttg gataaatcct gaggcttgcc tttaaagtaa tcatctaagc
     8101 aagcaataac tgggtctaaa ataggatgac taacaacttc ttctatcgtt tcatctccta
     8161 gtcccctctc aaaatgcttc tgctcctgaa cccaaattcc atacaaataa tggtcatcag
     8221 ctacaagtga tagaatacca attggtgagg agtagaggat ttttacatac ttcttttgca
     8281 ttatttcttt aatttttgat aggccaagcg ttcgccatct actggcacct taccaacttg
     8341 tttaaaacca agtttttcaa aaatatgttg cataaccttg ttttcagcat gcgtatctga
     8401 gcgaaaatca agataatcaa aaccttcaat caagccctct aagaaggttt gagcaactcc
     8461 ttttccctgc acatctgctg ccacagcaat acggtgaaag actagatact ctgactctcc
     8521 agcttgccag tttccctcat aaatagcttc ataggcctcc tctggactct tggtcacagc
     8581 agcataggct agcagttctc cctcttccaa ggctacgtag gcttgccctg agatgatatc
     8641 atcgataata acatctgcat ttggatagcc attttgccac tggttactac cagcatctgc
     8701 taaacatttt ttggcatcct ctatcacctg catgatggca tctacttcat ttggaaaagc
     8761 taaacgaatt tccatctttt ttcctcattt ctgactagtt tctcccatca tagcataatt
     8821 taagtctttc tacaagcagt taaaacttga ctttgtattt ttgttatttt ataatctagt
     8881 tatcaaaact atataaaaag gagttgaaaa tgaaaactac cttttcctac ccaaaatggg
     8941 cagaaattcc aaacattaac ctctatctgg accaggtttt gctctatgtc aatcaggtct
     9001 gcgcccctat ctctcctaat aaagacaaag gcctaacagc atctatggtc aacaattatg
     9061 tgaaaaatgg ttacctgaca aagcctgaca aaaaaaaata ccaacgccaa cagattgccc
     9121 gtttgattgc tatcacaacc ctcaagtctg tattttctat tcaagaaata gcccagacac
     9181 ttaatactct acaaactcaa gcaagttcag accaactcta cgatgctttt gtggactaca
     9241 tgaaccaagg aattgaccca gctaacccta ttatccaaac cagctgccaa accgttaaac
     9301 tctatcatca aactctagac ttaatcgatc atactcaaga ggaggtaatc caatgaacac
     9361 tagtcttaaa ctcagcaaac aactcagttt tggagaggag attgctaata gcgtgaccca
     9421 tgctgtgggt gcagtcatca tgcttatctt gctgcctatt tcatccatct atagttatga
     9481 agcacacgga tttttatcct ctatcggcgt ttccattttc gtcatcagtc tctttctcat
     9541 gttcctatca tccaccattt atcactctat ggcctatggt tcgacccaca aatatgtttt
     9601 gcgaatcatt gaccattcta tgatttacgt tgccattgcc ggctcataca cgcccgttgt
     9661 cttgaccttg atgaataact ggtttggcta tctgattatt gtcatccaat ggggaacgac
     9721 catctttggt attctctata aaatctttgc taaaaaggtc aatgagaaat ttagccttgc
     9781 tctttacctg attatgggct ggttggttct ggctatcatt cctgccatta tcagtcaaac
     9841 gacacccgtt ttctggagtc tcatggtaac tggcgggctc tgttatacag ttggagctgg
     9901 attttatgcc aagaaaaaac cttatttcca catgatttgg catctcttta tcctagctgc
     9961 gtccgcactt caatacatcg ctattgttta ttacatgtaa aaaagttgag aaattcaatc
    10021 tcaacttttt tctttacaca tattgataaa gtactggtgc aagcgcacat catcagtcaa
    10081 ttctggatga aaagaactta ccaacatatt tttttcttgg gctgcaacaa tttgattgtt
    10141 cactgttgct aaaatttcta caccctcacc aacactactg ataatcggac cacggataaa
    10201 ggtcattgga atcttgccaa ctcccttaca ttctgcttcc gtgtagaaac ttcctaattg
    10261 gcgcccataa gcattacgct cgaccaccat atccatagtt cctagatgac tctctttctg
    10321 agaagtgatt tccttagcca gcaaaattaa gcccgcacag gtcccaaaca ctggtaagcc
    10381 agatagaatg gcttctcgga tgggaagtag catgttctgg tcacgtaaga gcttgcccat
    10441 ggttgtagac tcaccaccag gcaaaatcaa acccgacaag tcactctgat cttgctgaaa
    10501 atcatctaga tttctgagtt ctacactctc gacacctaat tgatctagca cttttgcatg
    10561 ttctgcaaag gccccttgca aggccaatat tccgattttc atctattttc ctcgttcagc
    10621 catgagaatt tggatttcat tttcattaat accaaccatg gcttctccta aatcttcaga
    10681 gatttgagct agaatttgag gattacggaa gttagtcaca gccttaacaa tggcactcgc
    10741 tcgtttaaca ggatctcctg acttgaaaat acctgaaccg acaaagaccc cctctgcccc
    10801 taattgcatc attaacgcag catctgctgg cgttgcaaca cctccagcag cgaaatttac
    10861 aactggcaat tttccatgtt catgaacata ttggaccaat tctacaggga cttgcaaatc
    10921 cttggcagca acataaagct cgtcctcacg taagttttga atgcggcgaa tttcctgatt
    10981 catcatacgc atatgacgaa cagcttggac tatatcccct gtccctggtt ctcctttggt
    11041 acgaatcatg gaagcacctt cagcgatacg acgcaaggct tcacccaaat ccttagcacc
    11101 acagacaaaa ggaacttgga attctttctt gtccacatgg aaacggtcat cagctggaga
    11161 tagaacttca ctctcgtcga tataatcaat ttcaatagcc tctaaaatct gagcttcaac
    11221 aaaatgcccg attctgacct tagccattac tggaatacta accgcttctt ggatttcctt
    11281 aatcatcttt gggtcgctca tgcgggaaac tcctccagct gcacgaatat cagccggaat
    11341 tcgttccaag gccatcacag ctgccgcacc agcagcttct gcgatacgag cctgttcagg
    11401 attctgcaca tccataataa caccaccctt gagcatctgt gccaagtttt tatttagttc
    11461 ataacgattt tcagtcattt gttttatcct catcatttgt attggtagct accttttcat
    11521 tttaagtcag aacagaatga agcacaagat aaaaaaacta taattgtatg gggacagatt
    11581 agctaaaaac tatctgacct taacttaagg ccagatagct cattcatttt tatttttcag
    11641 ccgtaagggc agccattgtg atgtagttgt atggtttgtt gaagtgtggc aagaagaaga
    11701 ggtctgtcaa tgccaattta tcaattgtca catgctcttg gatagcaagt gagaacatgt
    11761 ggattcccat gctaattgca atatcatgtg aaaccatttg ggcaccaaga atttcacggc
    11821 tatctttgtc aaagacaatc ttaattgcta cttcatggtt gtcatgtttc atgaattctg
    11881 gtttttgaag atcgttaaag cctgtttcag ttgcgttgta accagcagct ttcgcttttt
    11941 caagagtcaa accagttgaa accatgtgaa gaccgtagat tgagatacca tttgaacctt
    12001 gaacaccgat tccttccaat tcatgtccac aagcattgta ggcaccaacg ataccagtgc
    12061 gaacagcatt tgaagcaaga gcgatatagc ttgtatcttt acgagcattg tcataaacag
    12121 tcgcacagtc accaacagcg taaacgcctg ggatagatgt ttcttgtttc ttgtctacaa
    12181 ggaaggcacc gttgcggaag agttcgatct taccatctgc aagggctgtg tttggacgga
    12241 aaccaactgc aaggataacc atatccacgt caaagctttc tttgtcagta atcaagcgtt
    12301 caactttacc gtcaccttcg attgctttaa cagtttgacc tagagccaag cggatgttgt
    12361 gatcttccaa gttcttcgcc atcatttgtg tgaagtcttt gtcatagtaa ccgttcaaga
    12421 cagtatcaac gatatcaaca aggacaactt cttttccaag acgttcaaag gcttcagcaa
    12481 gttcaacacc gatgtaacca ccaccaacaa cggcgatacg gtcgaggtgt tggctcttgt
    12541 cagaaagttt attgataact tcttcagcat tttggtacaa tttcacgaat tgtacgtttt
    12601 caagagttgc tttaaattcg cggtttcctt taacaatttc aacaccttcg attggtggca
    12661 agattggtgt agaacctgta gcgaaaatca atttttcgta tgattctttg tgctcttttc
    12721 cttcaacttc cgctgtaact actttgttat catagtcgat tgaaagaaca ggtgagttca
    12781 tgtaaacttt agcaccttta gcttccaatt tttctttatc agaatagaac aagccttcag
    12841 caccgtcaat ttgttcacca atccaaagag ccattccaca tcctaggaaa gagatgttag
    12901 agttttggtc aaatacaaca atttcgttct catttccaaa attatccaac atggtattga
    12961 tacatgctgt accagcgtgg ttagcaccga ctacaacgat tttactcata aaaatttcct
    13021 gccttcattc taaatttacc ttactagtat accatttacc gttagcgttt acaagggggg
    13081 tactattatt tcagcatttt ttatcaaaaa taattcccca tttatcctgt tttcaagcct
    13141 catgaatttt atgagcatat ttcttgactt tttccaaatt ttgtttgtga aaattctaac
    13201 tttatggtat tataataaca tgaaaatgaa atgtaagggc ttcaatttag atgattattt
    13261 gatattacaa cttattttct ataagagaaa ggagttggta gcttgactct tcgttcacat
    13321 tctgaacggc taatggggac tactatcact atttcattag tggatgagca agccgatatc
    13381 tttctccaaa aatcctttga cttgctcaaa gaacttgaat accgcttcaa cgccaatagt
    13441 caagaatctg agttgatgga aatcaattat caagcaggaa tagcccccgt cacggttcat
    13501 ccagacctgt ttgagctgat ttcacttggg ttagagcata gcctagcgct ctctagccac
    13561 ctcaatatca gcattggtcc cttaattcaa acctggcgta tcggtttttc agatgccaag
    13621 gtcgcccagc ctcaagaaat tgaatcggtg ctgcctctaa tcaatcctca tggtatcgag
    13681 ttagattctt ctacttctac tgtgttttta aaacagaaag gaatgaagat cgatctaggt
    13741 tgtttagcca aaggatacag tgcggataag gttgcccaat ttcttaggaa agagggggtg
    13801 acttctgcct tgatcaatct gggagggaat atcctgacca ttggaaaaaa tcaggcaaga
    13861 ggggataacc catggcaaat cgggattcaa gacccagcca atcctagggg aaatcattta
    13921 atgaccatcc ctgttgtcaa taaatctgtc gtgacttcag gcatttatga acgtcacctg
    13981 accgtcgatg gacaagatta ccatcacatt tttgacagtc aaacaggata tcctgttgaa
    14041 acggaactag cgagtctaac aatcatctct gataaatcag tcgatggcga aatctggacg
    14101 actcgactct ttggagaaag acctgcttct atcctctggc aagtcgaaag tttggagggc
    14161 atcgaagtca tcctcatcga taaagaaggc cacctaagct gttcttcagg aattcctact
    14221 ctatagagac aactgtttca atggaatttt aaaattgtat tatgtaaaaa gagaaaggaa
    14281 atctcatgtt aaaacttatt gctattgttg gaacaaattc aaaacgttct acaaaccgtc
    14341 aattgcttca atacatgcaa aaacactttt ctgacaaagc tgaaattgag cttgttgaaa
    14401 tcaaggccat tcctgtcttc aacaaaccag ctgacaagca agtacctgct gaaatattgg
    14461 aaattgctgc taaaatcgaa gaggcagatg gcgttattat cggtactcct gagtatgatc
    14521 actctattcc agctgttttg atgagcgctc ttgcttggtt gtcttatggt atttacccac
    14581 ttttgaacaa accaatcatg attacaggtg cttcttacgg tacgcttggt tcatctcgtg
    14641 cccaattgca acttcgtcaa atcttgaatg ctcctgaaat caaggcaaat gttcttccag
    14701 atgaattctt gctctcacac tctcttcaag catttaaccc aagtggcgac ttggttgacc
    14761 ttgatgttat caagaaattg gatgccatct ttgatgactt ccgtatcttt gtaaaaatca
    14821 cagaaaaatt acgtaatgca caagaattac ttcgcaaaga tgctgaagac tttgactggg
    14881 aaaatttgta agataggaga ccaaaaagaa tgaaatttgt tggacttgtt ggatcaaact
    14941 acgatcaatc atataaccgt aagctcttgg aatttatccg tcgctacttc aaactcaaat
    15001 ttgaattaga agttcttgaa attgacgaag ttccaatgtt taaccaagat gaaaaatggg
    15061 atgaaagttt ccaattacgt tatttatata acaaaattac tcgtgcagat ggtgttatca
    15121 ttgctactcc tgagcataac cacactatct cagcttcact caaatctgta cttgaatggc
    15181 tttcatacga agttcatcca tttgaaaaca agcctgttat gattgtgggt gcatcatact
    15241 atgaccaagg aacatcacgt gctcaagttc accttcgtaa aatccttgat gctccaggtg
    15301 ttaatgccta cacgcttcca ggtaacgaat tccttcttgg taaagctaag gaagcttttg
    15361 ataacaacgg aaatatcacc aacgaaggca ctgttaaatt ccttgaaact tgcttagata
    15421 actttgttaa atacgtagga gtcgtttcga aattgaaaaa accaaaacca atcgaatcag
    15481 aagacttgga ttgtggaaaa ccaatcgcta caaccatcac agaagttgac cctgacgatc
    15541 cagaatgggt agaaaaagtt gcagcaatca ctggtgctgt ttctggtgat acctatgtca
    15601 aattggacca cggtatccta acagttaacc aaattgatat gttcttgaaa gctatgccat
    15661 ttgaattgac atacgctgac gacaacaacc aattcctcta ctacaacaac gctcaccaag
    15721 atccagacac tatgttcgct aaacgtgtac cacctcaatc aggtagccgt atgtcgactg
    15781 ttcatggttc tcttccacca gcacgtatga agaacgtaga gtgggttatc ggaacacttc
    15841 gcaacggaaa ccaagaatac gtccgtacga tcgttccagg ttctcctgca ggtgttatca
    15901 acactcacaa ctaccaagca atgtactatc ctgatggatc atacgctggt atcaatgaaa
    15961 tcgtctttaa cttccaacca tggcttgact ggtacctaaa agagactggt caacgtttgg
    16021 tgggtggtag cggaccattt gctcctgctg ccggaggtca tggagacgcc gatgctactt
    16081 ctggcgcttc tgattcaggg gatgctggag gccacggtgg tgacgcagac gctacagccg
    16141 gtgcaagtta ctaatgatag tttttaggaa ccattatggt tcctttttta gcgaaaaaag
    16201 actagcaaac acaaactgct agtctctttg ttatcgtttc atctatcttt gttaaaagaa
    16261 agagtactta tactcaatga aaatcaaaga gcaaactagg aagctagccg caggctgtac
    16321 ttaagtacgg caaggtgaag ctgacgtggt ttgaatttga ttttcgaaga gtattattat
    16381 gaatttgggt catcaagact acggccatgt aatccttttt cacgttgtac ttggcgtagt
    16441 ttttctggtg taacatcgtt tccttcttcg tccacaattt tgattccttc aatgtggtga
    16501 cgaacagcgc ggcgataacc ttcgatgtac tcctcacgta gtttggcttg ttccactttt
    16561 tcttctgatg ttaagccttc tgtttttttc tttttagcaa gctcattgat acgagcaatt
    16621 tttttcggat ccatttcttc tcctttgtaa taagataaag tccgtttatc aaattttatt
    16681 tagtgttaat ttggttaaaa caagcaaact tagctgcttt cttggttagt tgtgacaaga
    16741 ttgccaaacg attttgacgg acagcctgat cttcagccat taccatagta ttttcaaaga
    16801 aagcatcaat gactgggcta agcgcaaaga gttgtttcaa ttgctgactt gcaggtcctg
    16861 ataaaacgag tgtttctact gcttctgcca aagctttttc ttggtcattc tcaaatagtg
    16921 ctgaatcaac tgtagcaacc ccttctgcct tctcagccag gttaaaggca cgagaaagtg
    16981 attcaacaga tggtttaaaa tcttcttcct tgcttacttc tacgagagca cttgctgctt
    17041 ccaacatatc tgccacaaca aagtttgaac ctgcaagaac tgcttccttg atatcttttg
    17101 gagtagagcc catcatctta tcaacacgag ccttgataaa gtccataacc tctgctttat
    17161 tttcataagt caaactgtca aatttcaatg cataaaggct atcaatcagc tcatccatag
    17221 caatgtgcca accaaaggca tccaagatac gaaccacacc ttgagttgca cgacgaaggg
    17281 cataagggtc attagaacct gatggaatca atcctactga gaagaaactc aaaatcgtat
    17341 ccaatttgtc tgcaatggct agaactgcgc cgaccttgct ctctggaagt tctccttcag
    17401 ctgatgtagg catgtagtgt tcacgaatag cagctgccac cgctggagtt tcaccagcaa
    17461 gaagggtgta tttttcaccc ataattcctt ggagttcgtc aaattcacca accatacctg
    17521 tcaacaagtc aaacttgtaa atggctgctg cacgagcaag gtcaactgtt tcatccactg
    17581 acaaaccagc tttttctgcc aaaagtacag tgatttgacc cgtacgaatc atgtgttcac
    17641 gaagggaacc aatcttctca tggaaggtga cattgtttaa tttttcaaca agatctgaaa
    17701 tcaccaattt ttggtcttca cgccagaaga attctccgtc ttccaagcgg gctaccaaga
    17761 ctttttcatt tcctttgatg acatttttca aacgctctgc gtttccgtta cgaacagaaa
    17821 tgaagtttgg caagagtttt ccatcttgat cacgaacaac aaagtaacgc tggtgttcct
    17881 tcatagaagt caccaagact tcttctggaa cttcaaggta tttagcatca aaacttccca
    17941 tgaaggcagt tgggtattca accaaattca agacttcatt cagcaaatcc gcatcaattt
    18001 cgatacgtac accatgtttt gcctcaattt ccttgatttg gtcaacaatc atttgctcac
    18061 gttcacatgg atctgcgatt acaaactgct tacgaaggtc ttcttcatag ctcaatgctg
    18121 actgaatttt ggtttctttc cccaagaaac gatggccacg gctcacacga cttcccttga
    18181 tatcaaggaa atccaagtca aactcttgct catccaagag aacagttaaa gtgtgaacag
    18241 ggcggatgta ttcaaagcta tttccagccc agtgcatgct gacagggaaa gtcaatgact
    18301 tcaagacatc tacaacacca ggaacaatgg cttcaactgc ttgaccaact tcttccttag
    18361 tgacatagac atattcttca cccttgattt cacggaattc gatatcttca acagtcaaac
    18421 ctttcccacg gacaaatcct tgagctgctt tggtgaagtt tccatcacta tccaaggcaa
    18481 ttttctttgc tggtcccttg aaatcttctg tcaaatcaga ctgtttgtct gataaaccag
    18541 tcacacgaac agccaaacga cgtggtgttg agaaggtttg aatggcttca aaagacaggc
    18601 ggttttcctt gaggaaggct gccatttttt cgcctagttg tttttcactt ggtgtgacaa
    18661 cataggctgg taattcttca agaccgagtt ctactaataa gttttttgtc atgttttttc
    18721 tccgaaaagt atctttttat tttccagttt gccttatgtg attggcacgc aagcaaccaa
    18781 cttcgttgtt tcattgctaa aattggagcc aaaagtctcc aattttgcct agtatcctta
    18841 gtttcactaa ggataccttc atcactgcgg caactggctc agggctcact ctgttcgccc
    18901 attttcgtaa tttgtcactc tctttattct gcgtcttctg ctaggagttt agctcttgtt
    18961 tcttcatcca aaagtgggta gcctaggcgt ttgcgttctg cgacaaaggt tttggctacg
    19021 acacgggcca agttacggat acgagcgata tagcctgcac gctctgttac agatacggca
    19081 ccacgcgcgt caagcagatt aaaggtatgt gaacatttga gaacatagtc ataggcaggg
    19141 tgtaccaagc cttcttctaa tgcacgacca gcttcttttt caaacttatc aaaattttca
    19201 agcaacattt cttggtccga aatttcaaat gaatattttg agtgctcata ctcaggctgg
    19261 ataaagattt ctccgtattt tacaccatca gcccactcga tatcatagac agagtcaact
    19321 tcttgaatgt aagaagccaa acgctccaaa ccataggtaa cttccgcagt cacaggacca
    19381 gttgccaatc caccgacttg ttggaaataa gtaaactgag tgatttccat cccatcaagc
    19441 caaacttccc aaccaagacc agctgaacca gtcgatggat tttcccagtt gtcctcaacg
    19501 aaacgaatat cgtgttccaa aggattgata cccaattttt ccaaagactc aaggtaaagc
    19561 tcttggatat ttgatggaga aggtttcata accacttgga attggtggtg ttggtaaagg
    19621 cggttagggt tttccccata acgaccgtca gcaggacgac gtgatggctc tacataagcc
    19681 gcattccatg gctcaggtcc gatagcacga aggaaagtgt aaggactcat tgttccagca
    19741 cctttttcgt tgtcataagc ctgcataagc atacaacctt ggtcattcca aaattgttgc
    19801 aaagtaagga tgatttcctg gaaagtcaat tttttagaca ttatttactc cttttaatta
    19861 aaaaaatttg ttttctttat ttcattttct tttaaaacta ttattgagaa attatttctc
    19921 tttttttgca tgtttatcaa tttatcaata ctacaaaaga cttcatt
//
LOCUS       DAVLSL010000023        19690 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLSL010000023 DAVLSL010000000
VERSION     DAVLSL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.023"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(188..1156)
                     /locus_tag="VXN30_000664"
     CDS             complement(188..1156)
                     /locus_tag="VXN30_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1357328.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            1292..2149
                     /locus_tag="VXN30_000665"
     CDS             1292..2149
                     /locus_tag="VXN30_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1357329.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            2136..2915
                     /locus_tag="VXN30_000666"
     CDS             2136..2915
                     /locus_tag="VXN30_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1357330.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            2939..4291
                     /locus_tag="VXN30_000667"
     CDS             2939..4291
                     /locus_tag="VXN30_000667"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1357331.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVNYYVDTITDVVRAEIGID
                     "
     gene            4365..4739
                     /locus_tag="VXN30_000668"
     CDS             4365..4739
                     /locus_tag="VXN30_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1357332.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            4741..5589
                     /locus_tag="VXN30_000669"
     CDS             4741..5589
                     /locus_tag="VXN30_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1357333.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            5855..6622
                     /locus_tag="VXN30_000670"
     CDS             5855..6622
                     /locus_tag="VXN30_000670"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1357334.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWIDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            6634..7818
                     /locus_tag="VXN30_000671"
     CDS             6634..7818
                     /locus_tag="VXN30_000671"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1357335.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            7815..9686
                     /locus_tag="VXN30_000672"
     CDS             7815..9686
                     /locus_tag="VXN30_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1357336.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKSDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLNKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMSRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(9829..11013)
                     /gene="mntE"
                     /locus_tag="VXN30_000673"
     CDS             complement(9829..11013)
                     /gene="mntE"
                     /locus_tag="VXN30_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1357337.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPILEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            11317..14013
                     /locus_tag="VXN30_000674"
     CDS             11317..14013
                     /locus_tag="VXN30_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1357338.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            14026..14817
                     /gene="yghU"
                     /locus_tag="VXN30_000675"
     CDS             14026..14817
                     /gene="yghU"
                     /locus_tag="VXN30_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1357339.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            14933..15343
                     /locus_tag="VXN30_000676"
     CDS             14933..15343
                     /locus_tag="VXN30_000676"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1357340.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            15355..16950
                     /locus_tag="VXN30_000677"
     CDS             15355..16950
                     /locus_tag="VXN30_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357341.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            17016..17180
                     /locus_tag="VXN30_000678"
     CDS             17016..17180
                     /locus_tag="VXN30_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1357342.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            17259..17987
                     /gene="yaaA"
                     /locus_tag="VXN30_000679"
     CDS             17259..17987
                     /gene="yaaA"
                     /locus_tag="VXN30_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1357343.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(18038..19522)
                     /locus_tag="VXN30_000680"
     CDS             complement(18038..19522)
                     /locus_tag="VXN30_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1357344.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILELLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
BASE COUNT         5783 a         3673 c         4491 g         5743 t
ORIGIN      
        1 tcaaaagggg caaaaaaggg gcaaaagtgt cgtaaacctc tgtaaaatga tgtaaaaaca
       61 tttatttaaa agctcaaaat atagctgttt taaaaggtat tgtaagatat agtaaaacga
      121 tgtaaaggta tttttaaatg cggatgaatt ataaaaccac gaatcctatg tgactcgtgg
      181 ttctttttta taaactagta gagtgttttg gttgtacttt ttgttcaggg tcaatgtagt
      241 tgatagcgtt gttgacagca gttggagctt ctccgaggcc tgtcgcaatc agatcaattt
      301 ttccgtcata gtagcagcag tcaccgatag catagatacc tgcttggctg gattcctgtt
      361 tgctgttgac gataatcttg tgacggttga ggtcgagccc ccagttttta aggttaccga
      421 cagaagattt gaaaccatag ttgacaaaga ggtggtctag gtcaatggtt tcagtttcat
      481 cagatttgac ttttgtgatt tcaagtttat caagtgtttt tccatttcca aggagttggc
      541 taggggcgaa tggtgtcttg atggttacag atgattcttg caaggcttga acactgtgtt
      601 ccaaggcacg gaaattatct ctgcggtgaa caagggtagt tggtgcgatt ttttcaaaag
      661 ccaaagccca atccacagcc gagtctcccc caccaagaat cgtcactttc ttaccagcgt
      721 attgctgaat gttagaaacg tggtagtgga tattttcata gccctcaaca ccttcaagtt
      781 ccagcggacg tggtttgaag gcaccgccac ccatagcgat gatgactgtt ttagtcaggt
      841 gacttccttt agaagttgtg atggcaaatt cttcttcttg tttgtcaatc tcaagaaccg
      901 tttcattgag atgaataggg gtatcaaatc catttagctg ttcaatcaag cggttagtca
      961 actcttctcc agtcaggttt gggaagcctg gtacgtctag gatttccttt tcagggtaga
     1021 gaatagcagg ttgtccacct agctggggaa gagagtcgat gatttgaacc ttggcttggc
     1081 gtaggtgggc ataaaaggct gcaaaaagcc cgacaggacc accacccaca atggtaatat
     1141 catagagttg agacatagtt tctcctttgt tttttctagt cagtttattt tatcatattt
     1201 ttgttcaatg taaaatggac taggataggg aaaaaaatgc cagaaaatgg tataatagga
     1261 cgaaagtgtt ttgataggga ggatatcagg gatgaatttt caacaattat ccaatctgca
     1321 atattggacc agtttgtttg caagtccatg gacgatagct atcaatctga ttgatatttt
     1381 gattgttgct tatattttat accattttac aaaagctatt gcaggaacca agattatgat
     1441 tttggtacgt ggagttttgg tgtttatttt agctcaaatc cttgcaaata tgattggttt
     1501 gactacgatt tcttggttaa tcaatcaaat tattacttat ggggttattg cggcggttgt
     1561 tatcttctct ccagagattc ggactggttt ggaacgtttg ggaagagcga cagatttctt
     1621 ttccaatgcc cctattagtg ctgaggaaca gatgattcgt gcctttgtta agtctgttga
     1681 atacatgagt cctcgtaaaa tcggggcctt ggttgctatt cagcgtgtac gtaccttgca
     1741 ggagtatatt tcgacaggaa ttcccttgga tgctaagatt tctgcagaac ttctcattaa
     1801 catttttatt cccaacactc ccctacatga tggtgcggtg attatcaaag aagaacgtat
     1861 cgctgtgacg tctgcctatc tgcccttgac aaaaaacaca gggatttcca aggaatttgg
     1921 gaccagacac cgggcggcta tcggtttatc agaagtctca gatgccttga cttttgtcgt
     1981 atcagaggaa acgggaggaa tttcgataac ctataatgga aggtttaagc acaacctaac
     2041 acttgatgaa tttgaaacag aattacgtga aatcttactt ccaaaagagg aagtgggtct
     2101 tagttttaaa gaacgattgc taggaggatg gaaacatgaa aaaaaatagt ttatatatca
     2161 tatcctcact cttttttgct tgtgtcttat ttgtctatgc tacggcgacg aattttcaaa
     2221 acagtaccag tgctaggcag gtaaaaacgg aaacctatac taatacagta acaaatgtcc
     2281 ctattgacat acgctataat agtgataagt attttattag cggttttgct tcagaagtat
     2341 cagtggtctt gactggtgca aatcgcctat cgctagctag tgaaatgcaa gaaagtacac
     2401 gtaaattcaa ggttactgct gacctaacag atgccggtgt tggaacgatt gaagttcctt
     2461 tgagcattga agatttaccc aatgggctga ccgctgtggc gactccgcaa aaaattacag
     2521 tcaagattgg taagaaggct cagaaggata aggtaaagat tgtaccagag attgacccta
     2581 gtcaaattga tagtcgggta caaattgaaa atgtcatggt gtcagataaa gaagtgtcga
     2641 ttacgagtga ccaagagaca ttggatagaa ttgataagat tatcgctgtt ttgccaacta
     2701 gcgaacgtat aacaggtaat tacagtggtt cagtaccttt gcaggcaatc gaccgcaatg
     2761 gtgttgtctt accggcagtt atcactccgt ttgatacaat aatgaaggtg actacaaaac
     2821 cagtagcacc aagttcaagc acatcaaatt caagtacaag cagttcatcg gagacatctt
     2881 cgtcaacgaa agcaactagt tcaaaaacga attaaaaata gaaaaggatt ttataacaat
     2941 gggtaaatat tttgggactg atggagtccg tggagaagct aacctagaac taacaccaga
     3001 attagccttt aaactaggac gttttggagg ctatgttctt agtcaacatg aaacggaagc
     3061 gccgaaagtc tttgtaggac gtgacacacg tatttcaggg gaaatgttgg aatcggcctt
     3121 ggtggcaggt ctcctttcag tagggattca cgtatacaaa cttggtgtcc ttgcaacacc
     3181 agcagtagct tacttggttg aaactgaagg agcaagtgcc ggtgtcatga tttctgctag
     3241 ccacaaccca gcccttgata acggaatcaa gttctttggc ggtgatggct tcaaactaga
     3301 tgatgaaaaa gaagcagaaa ttgaagcctt gctagatgct gaggaagaca ctcttcctcg
     3361 tccaagtgca gaaggcttag gaattttggt agattatcca gaaggcttgc gtaagtatga
     3421 aggatacctt gtttcaactg gaactcctct tgatggaatg aaggttgcct tggatacagc
     3481 taatggagca gcttctacca gtgcccgtca aatctttgca gaccttggtg cccaattgac
     3541 ggttatcggg gaaacaccag acggtcttaa catcaacctt aatgttggtt caacacatcc
     3601 agaagccctt caagaagtgg tcaaagaaag tgggtcagct attggtttgg cctttgatgg
     3661 agacagtgac cgcttgattg ctgttgatga gaatggtgac atcgttgatg gtgacaagat
     3721 tatgtacatc atcggaaaat acctttctga aaaaggacaa ttggctcaaa atacaattgt
     3781 gacaactgtt atgtctaacc ttggtttcca caaggccttg aatcgcgaag gtattaacaa
     3841 ggcagttact gcagttggtg accgctacgt tgttgaagaa atgagaaaat caggctacaa
     3901 ccttggtggt gaacagtctg gtcacgttat cttgatggat tacaatacca caggtgatgg
     3961 tcaattatca gcagttcaat tgactaaaat catgaaggaa actggtaaga gcttatcaga
     4021 gttggcggca gaagtaacga tttatccaca aaaattagtt aatatccgag tggaaaacgt
     4081 catgaaggaa aaggccatgg aagtgccagc tatcaaggcc atcatcgaga agatggaaga
     4141 agaaatggcg gggaacggcc gtatccttgt tcgtccaagt ggaacagaac ccctcttgcg
     4201 tgttatggca gaagcgccta caacagaaga agtaaactac tatgttgata ccatcacaga
     4261 tgtagttcgt gctgaaattg ggattgacta aatcctagaa aactagatga aaataaaaaa
     4321 ttatggtaca atagcttata atattgtagc cgatggagaa agacatgaat cttaaacgtg
     4381 aacaagaatt tgttagtcag tatcattttg atgcgcgtaa ttttgaatgg gaaaatgaaa
     4441 atggagctcc tgaaaccaag gtggatgtga atttccaatt gctccaacat gaccaagaaa
     4501 atcaagtgac ttccttgatt gtcatcttga gctttatgat tgtatttgat aagtttgtca
     4561 ttagtggaac aatctcacaa gtaaaccata tcgatggccg tattgtcaat gaaccaagtg
     4621 aattgaacca agaagaggtg gaaaccttgg ctcgtccatg tttgaacatg ctcaatcgtt
     4681 tgacttacga agtgactgaa attgcattag acttaccagg aattaatttg gagttctaat
     4741 atgaaattag ctgttttcac agattcctct gcttatctca gtgcagagat cttgcaaaga
     4801 gaagacttgt ttgtcttgga tattcctgtc aatattgatg gtgaggaata tgtcgaaggc
     4861 atcaatctgt ctgctgagga attttaccaa aaaatggctc aggcttctga attgcctaag
     4921 accagtcaac caagtattgc caagttagat gaaatcttaa cttcgctcaa agaacaaggc
     4981 tatacacatg ccttggggct tttcctatct tctggaattt caggttttta ccaaaatatc
     5041 cagtatatgg tcgatgacta tgagggctta accattgctt tcccagacac tttgattaca
     5101 agtgctcccc taggcatcat ggttgaaagc gtctttaatt ggcgtgatca gggcgatgat
     5161 tttgccagca ttcaggataa gctagccatt caaatcagcc gtacgtcagc ctttatcatg
     5221 gtagatgact tggatcattt ggtaaaaggt ggacgccttt caaatggggc tgccattttg
     5281 ggcaatttgc ttagcattaa gccaatcctt tattttaacg atcaaggtgt gattgaagtt
     5341 tacgaaaaag ttcgtactga aaagaaggcc accaaacgct taattgaaat tatcaaggaa
     5401 acaacggctt caggtcaata ccgggtcatt gtcattcatg ggaatgctcc tgaaaaggct
     5461 gaagaattgc gtcagcattt gcttgatttt ggcttgggtt cggatgtttc acttgctaca
     5521 tttggtagtg tcattggaac gcacctagga gcaggaagta ttgctctagg ttatattcca
     5581 gtgatttagt tggcacttct aggctagtta atcttagaaa ttgaacacgg actacctgcc
     5641 tcttgaaaaa agatgcctgt cttgcctttc gtggaaagtc agtgccattc cctatttttc
     5701 aggggcagct aaggtccttt gtatcttgat aattgaacac gcctggaacc ctgtgtgaaa
     5761 aagatagttc ttccaagaag tagacacttc ttgatcagaa ttcctatttt cactttgtgt
     5821 tcttacaggc tttgtatttt ggacaggagt agagatgagt attcgagtaa ttattgccgg
     5881 ttttaaggga aagatgggcc aggctgcttg tcagatggta ttgactgatc cagacttgga
     5941 cttggtagca gttttggatc cttttgagtc tgagtcagaa tggcagggta ttcctgtttt
     6001 caaggataag gctgatttag ctggttttga agcggatgtc tggatagatt ttactactcc
     6061 agctgttgcc tacgaaaata cacgttttgc tcttgaaaat ggctttgctc cagtagttgg
     6121 aacgactggt ttcacgagtg aagaaattgc agagctaaaa gaattttctc gtgcccaaga
     6181 cttgggtggc ctgattgccc ctaactttgc cttgggtgct gtcttactca tgcaatttgc
     6241 gacgcaggct gccaaatatt tcccaaatgt ggagattatt gagctccatc atgacaagaa
     6301 aaaggatgct ccgagtggaa cagccattaa aacagctgag ttgatggcag aggttcgaga
     6361 gtcaattcag caaggtgcag cagatgagga agagctgatt gctggtgctc gtggtgctga
     6421 ctttgatggt atgcgcatcc actcagttcg tttgccaggc ttggtagctc atcaggaagt
     6481 catctttggc aatcagggag aagggttgac cctccgtcat gactcctatg atcgcatctc
     6541 cttcatgaca ggagtcaatt tgggaattaa agaagttgtc aagcgtcatg agcttgtcta
     6601 tggattagaa cacttattat gagattaacg caaatgcctt ctgaatttca gaaggcttta
     6661 ccagtattag aaaaaattaa agaagcaggc tttgaggctt attttgttgg gggctctgtt
     6721 cgagatgccc ttctccatag ccctatccac gatgtggata ttgcgacgtc ttcttatcca
     6781 gaagagacca agcagatttt tccgcgaaca gccgatatcg gaatcgagca tggaaccgtc
     6841 ttggtcttag atggggatga ggagtatgag gtaacaacct ttcggacaga ggatgtctat
     6901 gtggactatc gcagacccag tgcggtttcc tttgtgcgct cgctagaaga agacctcaaa
     6961 cgccgtgatt tcacagtcaa cgcctttgcc ttggatgaga caggagaaat cgttgacttg
     7021 ttccatggtt tagaagattt ggaaaagcaa gtcttgcgag cagttggagt ggctagtgag
     7081 cgtttcaacg aagatgcttt acggattatg cgtggtttcc gttttcaggc tagtcttggt
     7141 tttgcacttg agccagaaac atttaaagct atgaagacct tgacgccgct tttggagaaa
     7201 atttctgtag agcgtacctt cgtcgaattt gataaactct tgctggctcc attttggaga
     7261 aggggcttgg cttccatgat tgagagtcaa gcttatgact atctccctga tatggcatct
     7321 agccaggaca agctcaacag actgtttgat ttggagactg attttacttt tgaatcctct
     7381 gaacaagcct gggcggcttt actatgggct ttggagattg aaaatgcgca gtcatttttg
     7441 aaatcttgga agacctcacg ccagtttgcc aagcaagttc aggatttgct gattattttg
     7501 gctctgcgtg aaaatggaga attgagcaag cgagattgtt atcgctttga catagatttg
     7561 cttttacagg ctgaaaatct tcgtcaagct caaggaaaag aagtcaaccc acaagccatc
     7621 acagaaaaat accaaagctt gactatccat gacaagaaag agattcagat taatggcgat
     7681 attttgatca aggaatatgg ctatcagcca ggcccagact tgggagagat tttaacagag
     7741 attgagtttg ccattgtcga tggagaattg gagaataatc gtgaagccat ccatgcttac
     7801 ctgagggaga aaaaatgagt gattttatcg ttgaaaaact aagtaaatcc gttggtgaca
     7861 agaccgtttt tagggatatt tcctttatta tccatgactt agacagaatt ggtttaatcg
     7921 gtgtcaatgg gactggcaag accacccttt tggacgtcct ttctggtgtt tctggatttg
     7981 atggggatgt cagtcctttt tcagctaaaa gtgattacca gattggttat ttgactcagg
     8041 atcctgattt tgatgataga aagacagttt tggatacggt tctatctagt gaactcaagg
     8101 aaatccagct cattcgtgag tatgaattga ttatgctcga ctatagtgag gacaagcagg
     8161 cgcgtttgga acgtgtcatg gcagagatgg actctctcca agcttgggaa atcgagagtc
     8221 aggtcaagac cgttcttaac aaattgggca ttcaagactt atctactcct gttggggaat
     8281 tgtcaggtgg tctgagaaga cgggtacagt tggcacaagt cttacttggc aaccacgacc
     8341 ttttgctttt ggatgagccg accaaccatc tggatattgc gattattgag tggctgaccc
     8401 tctttttgaa aaattctaag aagaccgtcc tttttatcac tcacgatcgt tatttcttag
     8461 acgctttgtc aacacggatt ttcgagttgg agcgagcagg cttgacagaa tatcagggca
     8521 attaccagga ctatgttcgc cttaaggcag aacaggacga gcgcgatgca gctctacttc
     8581 ataaaaaaga acaactctac aaacaagaac tggcctggat gagcagacaa ccgcaggcgc
     8641 gtgcgaccaa gcaacaagct cgtatcaatc gtttccatga tctgaaaaag gaagtttcag
     8701 gcagtagtgc tgagacagac ttgactatga actttgaaac cagtcggatt gggaagaaag
     8761 tcatcgagtt tcaggatgtt tcctttgcct atgaaaataa gcccattttg caagatttta
     8821 atctcttagt tcaggctaaa gaccgtattg gaattgttgg ggacaatggt gttggaaaat
     8881 caaccctact taacctgatt gcaggaagtc ttgagccgac agcaggacaa gttgtgattg
     8941 gggaaactgt tcgcatcgcc tatttctctc aacaaattga gggtttggat gaaagcaagc
     9001 gtgtgatcaa ttacctgcag gaagtggcag aggaggtcaa gaccagtggt ggttctacga
     9061 cttccatcgc tgagttgctg gagcaattcc tcttcccacg ttcgacgcat gggaccttga
     9121 ttgagaagtt gtcagggggt gagaaaaaac gtctttatct cctcaaactg cttttggaaa
     9181 aaccaaatgt tcttctttta gacgagccaa ccaatgacct agatattgca actttgacag
     9241 tcttagagaa tttcttgcaa ggttttgcag gtcccgtttt aacagtcagt cacgaccgct
     9301 atttcttgga taaggtagcg accaagattc tcgcttttga ggatggcaag attcgtcctt
     9361 tctttggtca ttacaccgac tatcttgatg aaaaagcttt tgaaacagat atggccaatc
     9421 aagtgcaaaa ggccgaaaag gaaaaagtgg tcaaggttcg agaagacaag aaacgcatga
     9481 cctaccaaga aaagcaggag tgggcaagta ttgaaggtga tattgaaacc ttggaaaaac
     9541 gtatcgctgc tattgaagag gaaatgcagg ctaacggctc tgactttggt aagctggcta
     9601 ctctccaaaa agaattggat gagaaaaatg aagcactcct tgaaaaatac gaacgctatg
     9661 agtatctcag tgaatttgat agttaagact attcgaaaat caaagtgcaa actatgtcgg
     9721 cttcacattg ccgactcaag tacagcctgc gaccggcttc ctaatttgca ctttgatttt
     9781 tattgagtat aaacgaatag aaaaatctcg tctcatgaaa ggatttctct attctttttt
     9841 ggtttcttga tgaaagatat tttgccaggt ttcgtggcga cgccagatac tggtatggat
     9901 gataccatct aactcatagc agatgagttt ggttttttga ctgatggaag taatttgaat
     9961 gtccttgata gcagaattta actctttttt ggtcttataa gcctctttat ccatctgctc
    10021 tccatcttgg cgaatataga caaaatcatc agtcaagagt tcttctagtt ggtttccttg
    10081 gtcaatcaat tgttcacgca taagcaattt tttatagaca ttgtctaaaa tttcatcctc
    10141 aaggataggt gctggttcga tatggacatc ggtatcaaag acgccaaaac gctcctccag
    10201 catagactcg acctgatccg cgatttcatg gctttcaaaa acagacaagt caggattcat
    10261 ctctagtgta atatccaggt agatgttgct accgtaggtg cgacctcttt gcgatttgac
    10321 cttgctgatt ttgggaattt ccatgatagc cttttggtag tcctcgagca ggcggtcgtc
    10381 aaagccatct gaaagactaa aggaagactc gatgaagata tcataggcag tcttcaagat
    10441 aaagaaagtg atgatgatag caaccagttt atccacaatc ggataattga aactactagc
    10501 taggatggca atggcggttc caagtgaggt aacagcgtca gaaagattgt ccttagcagc
    10561 tgccttcagc gccttggagt tggatttctt actgaggcga gtattgtaga gatagaccac
    10621 aaacataatc gctgcagaaa tgattcctag agttgcacca agaggatcaa tgaccgtttc
    10681 ttcccgactg agaatctttt gaatggtatc tcttagaaca tcgaaaccga catagaacat
    10741 gatgatagaa gtgatcaagc ttgccaaatc ttcaatcttc caatgaccaa aacggtggtc
    10801 acggtctgca ggctggcgcg ccatccgaat cccgattaag agggctacat ttccaatgat
    10861 gtccgatacg ttattaaaac catcggccac caaactggat gaatgaagga gatgaccagc
    10921 tgctaatttg gctgcagaca agatcaaata ggtcgaaata ctgataatgg ctccacgctc
    10981 agctaacttg agatttgaga tagattgctt cattcaactt cctttctagt cacatagcat
    11041 tctaccatta tactggtttt caaggaaaaa ttcaaataag gcagttttta ctctttgaaa
    11101 actccccaaa atttggtata atggtactac tcaagggagt agctggcaga aacctgtgat
    11161 agtgtcgtca ttccgaattt tatgctgaaa agtatgcttt ccggccctat cttaaacagc
    11221 gagacttgtt atgattaaca ggtctctttt tgtttgtcgt gaaaagatat gacagaaaga
    11281 gagtctgttt tgcacacaat gtcaaggagg agacacatgt caaaagaaca aaaacgccaa
    11341 gcgttttata ctcagagccc tgaagaggtc ttgcaggctg tggatgcgac cgagcaaggt
    11401 ttgtcatcaa gtgaggcgga aaagcgcctt gccgaatttg ggcacaatga actcgaagaa
    11461 ggcgagaaac gatcaatctt ggtcaaattc atcgagcaat ttaaggattt gatgattatc
    11521 atcctagttg cggcagcaat cttgtcagtc gtgacttctg gtggggaaga tatcgcagat
    11581 gccattatca tcctagctgt ggttatcatc aacgctgcct ttggtgttta ccaagaagga
    11641 aaagctgaag aagctattga agccctcaaa tccatgtcta gtccagttgc ccgcgttctt
    11701 cgtgatggac acatggcaga gattgactct aaagaattgg taccaggcga tatcgttgcc
    11761 cttgaagcag gtgacgtggt accagcggac ctacgtttga tagaagccaa ctctcttaaa
    11821 attgaagaag cagccttgac aggtgaatct gtaccagtcg aaaaagactt gtcagtcgag
    11881 cttgcgacag atgctggtat tggtgaccgt gtcaacatgg ccttccaaaa ctcaaacgta
    11941 acctatggtc gtgggatggg tgttgttgtc aatacaggta tgtacactga agttggtcat
    12001 attgctggta tgcttcaaga tgcggatgag actgatacac cactcaaaca aaatttgaac
    12061 aacctttcta aggtcttgac ctatgctatc ttggtcattg cccttgttac ttttgtagtg
    12121 ggtgtcttca ttcaagggaa aaatccactt ggtgagttgt tgacttctgt tgcccttgcc
    12181 gttgcagcca ttccagaagg acttcctgct atcgttacca tcgttctttc tcttggtact
    12241 caagttttgg ccaaacgaca ttccatcgtt cgtaagttgc cagcagttga aacacttggt
    12301 tcaactgaaa tcatcgcttc tgataagact ggtacgctga ctatgaacaa gatgacagtc
    12361 gaaaaagtct tctacgatgc ggttctacat gactcagctg atgatattga actaggtctt
    12421 gaaatgccac tacttcgttc agttgtcttg gccaatgata cgaaaatcga tgtggaaggt
    12481 aacttgattg gtgacccaac cgaaacagcc tttatccaat atgccttgga caagggctat
    12541 gatgtcaaag gtttcttaga gaaatatcct cgtgtagctg aattgccatt tgactctgac
    12601 cgtaagctca tgtcaacagt tcacccattg ccagatggtc gtttccttgt agcagtcaag
    12661 ggtgcgccag accaactctt aaaacgttgt cttcttcgtg ataaggctgg ggatattgct
    12721 ccgattgatg agaaggttac aaatctcatt catacaaaca attctgaaat ggctcatcaa
    12781 gccttgcgtg tccttgcagg tgcttataag attatcgata gtattccaga aaatctcact
    12841 tctgaagagc ttgaaaatga tttaattttt actggtttga ttgggatgat tgaccctgaa
    12901 cgtcctgaag ccgctgaggc tgttcgtgtg gctaaggaag cgggaatccg tccaattatg
    12961 attacaggtg accaccaaga cacagcggaa gccattgcca aacgtttggg aatcattgac
    13021 gcaaacgata cagaaggtca cgttttaact ggtgctgaac tcaatgaact gtcagatgaa
    13081 gaatttgaaa aagtcgttgg tcaatactct gtttatgccc gtgtgtctcc agaacacaag
    13141 gttcgtatcg tcaaggcttg gcaaaaacaa ggtaaagtcg ttgccatgac aggtgatggt
    13201 gtcaatgacg cgccagctct gaaaacagcc gatatcggta tcggtatggg aatcactggt
    13261 acagaagttt ctaagggggc ttctgacatg attcttgcag atgataactt tgcgactatt
    13321 atcgtcgcag tggaagaagg acgtaaggtc ttctcaaaca ttcaaaagac tattcagtac
    13381 ctactttctg ctaatactgc tgaagtatta accatcttcc tatcaacctt gtttggttgg
    13441 gatgtcttgc agccggttca tcttttgtgg atcaacttgg taacggatac cttcccagct
    13501 attgctcttg gtgttgaacc tgcggagcct ggtgtcatga atcataaacc acgtggacgc
    13561 aaggcaagct tcttctcagg tggtgttttg agttctatca tttatcaagg tgtactccaa
    13621 gcagctcttg ttatgagtgt ttatggcctt gcgattgctt acccagttca tgtgggtgac
    13681 aatcatgcta ttcatgcaga tgccctaacg atggcctttg caacccttgg tttgattcag
    13741 ctcttccatg cctacaatgt caagtctgtc taccaatcca tcttgacagt tggcccattc
    13801 aagtctaaga cctttaactg gtccatcttg gtatccttta tccttctcat ggcaacaatc
    13861 gttgtagaac cgcttgaagg aatcttccac gtaaccaaac tagacttgtc acaatgggga
    13921 attgttatgg ctggaagctt ctcaatgatt atcatcgtcg aaatcgttaa gtttattcaa
    13981 cgcaaactcg gttttgacaa gaatgcgatt taagggaggt ttcggatgtc agcatatcaa
    14041 ttaccgaccg tatggcagga tgaagctagt aatcaaggag cttttacggg gctaaacaga
    14101 ccaacagcag gtgcccgttt cgaacaaaac ttgccaaaag gagaacaagc ttttcagctt
    14161 tattcactgg gaacaccaaa tggtgtgaag gttactatct tattggaaga attactagaa
    14221 gctggtttta aggaagcggc ttacgacttg tataagattg ctatcatgga tggggatcaa
    14281 ttcggctcag actttgtgaa gctcaatcca aattccaaga ttccagcctt attggaccag
    14341 tcaggtactg aaaacgtaag agtctttgag tctgctcata ttcttcttta ccttgctgag
    14401 aaatttggag cctttttacc aagtaatcct gtggaaaagg tagaagtttt gaattggcta
    14461 ttctggcaag caggtgcagc accttttcta ggtgggggat ttggacattt cttcaattat
    14521 gctcctgaaa aattggaata tcctattaac cgttttacga tggaagtgaa acgccagttg
    14581 gatttattgg ataaggaatt ggctcagaaa ccttatattg caggcaatga ctatacgatt
    14641 gcagatattg ctatctggtc ttggtatgga cagttagttc aaggaaatct ttaccaaggt
    14701 tctgcaaaat tcttggatgc ctcaagttat caaaatctag taaaatgggc agaaaaaatt
    14761 gccaatcgtc cagctgttaa gcgtggcttg gaagtaactt atacagaaat taaatagcaa
    14821 aaaagtcatc ttcggatggc tttttgctac agttgaggga aagggtttgc agttatcggc
    14881 ttttgtgcta taattgctat aatatagtaa aaagcaagag gagtgtgagg aagtggaaaa
    14941 gaaaattgtg aaggatatct tatttttatc tcaagtgtct cagccggcaa gtcaggagga
    15001 cctttatctt gccagagatt tgcaggatac actcttagca aatcgtgata cctgtgttgg
    15061 tctagctgcc aatatgattg gggtgcagaa gcgcgtgatt atctttaatc ttggcttggt
    15121 tcccgtggtc atgtttaacc cagtgcttct gtcctttgaa ggatcctatg aggcagaaga
    15181 aggctgtttg tccttggtag gtgtgagatc aactaagcgt tatgaaacca taagggttgc
    15241 ctatcgtgac agcaagtggc aggaacagac cattaccttg acaggcttcc cagctcagat
    15301 ttgccagcat gagctggatc acttggaagg acgaatcatt taggaggaaa gcaaatgaaa
    15361 cgaatagtct ttgaacttat ttttatcgca acgacctggt atatcttttt accgcccctt
    15421 aacctgacca gctgggaatt tctcttcttc ctctgtgggc atttgttagt tgtggcaatc
    15481 ttatttggct ttggcaaggg gataaacctt gtcaaaacgg ttcatgtgcg ccacggtaag
    15541 gcggaagctg ccttaaatct tgagggtttc aaaatcaatc ggttagggaa aattctgtta
    15601 gcttcgattg gaggaattct tctcttggca gctttggttt ccttggtaac ttccagcatg
    15661 tttcaggcta aaaattatgc caatgtagtc acggttacgg aaaaagactt tactgaattt
    15721 cctaagagtg acaccagtaa ggttcctatc ctagatagaa gtactgctga aaaaattgga
    15781 gaccgctact tgggttccct aaccgataag gtgtcgcaat acgtagcggc agatacctat
    15841 acccaattga caattgatgg gaaaccttat cgggtcacac cactagaata tgcagaccct
    15901 atcaaatggt ttaacaatca agccaaggga atcggtgagt atatcaaggt ggacatggta
    15961 actggaaatg cggatttggt ggacttgaag acaccaatca agtattcaga ctcggagtat
    16021 tttaaccgtg atgtcaaacg tcacctgcgc ttgaagtacc cgaccaaaat ctttaaaact
    16081 ccatcttttg aggtggacga tgagggcaat cctttctatg tagcaacggt ttaccaaaag
    16141 caatttggac ttgctgttcc tcgtcctgct tcagtcatta tcttggatgc tacaaatgga
    16201 gaaaccaagg aatacagctt atcagatgtt ccagaatggg tggacaggat ctatccagca
    16261 gaggaaacca ttgagcaaat taactacaac ggcaagtaca aggacggttt cttgaatgcc
    16321 atgatttcca agaaaaacgt gacccagact accaagggct ataattactt gtctatcggt
    16381 aatgacatct atctctacac aggtgtgacg tcggctaatg cggatgagag taatcttggt
    16441 ttcatccttg aaaatatgcg aacaggagaa atcactaagt atagcttggc ttctgcgaca
    16501 gaagaatcag cccgtgaatc agcagaaggt gctgttcagg agaaatccta caaagcaacc
    16561 ttcccaatcc tcatcaacct caatgacaag cctctctaca tcatgggctt aaaggacaat
    16621 gctggcttgg tcaaagagta cgccctggta gacgcagtcg agtaccaaaa tgttatcgtt
    16681 gctactacag tggaagagat gctcagcaag tatgccaata aaaacgacct tgaaattgac
    16741 aatgcaacga cagaaagcat caagggagta gtagcagacc tcaaatcagc tgttatcaag
    16801 ggagacactg tctacttctt taaagttgat ggcaagatat acaaggtcaa ggcttcagta
    16861 tccgatgatc ttccttacct tgaaaatggt aaaaccttcg aaggtcaagt aggaaaagac
    16921 aattatctca agacctttaa gctacggtaa aaataggttt tttcagaaag tatatgttat
    16981 aatagagtaa attaagacta atggaggaca aagaaatggg tttttatttg atggttgctt
    17041 caatgctact cggactgctc gctttgaaga taggtttttc tcagttcaag gagaaaaaag
    17101 ataaattctt atctatctta acaagtttag ctggcacagc ccttgttctc gtggctgtct
    17161 ggttaggatg gcccaaataa atagaaaaac ctcggtcaaa ccgaggtttt tcaaatgaat
    17221 ttcttggttg tggctaaaaa atatgctaca ctatcaatat gaaaatttta atcccaacag
    17281 caaaagaaat gaacacagac ttgccaagta tcgaggcaat tcctttaaaa ccagaaagtc
    17341 agaccgtgct tgatgccttg gctctctatt ctgccagtca attggagagt ttctacaagg
    17401 tatcagctga gaaagcggcg gaagaatttc aaaatatcca agctttgaaa aggcaaactg
    17461 ctcaacacta tccagccttg aaactttttg atgggcttat gtaccgcaac attaagagag
    17521 ataagctgac cgaggcggaa caagattatc ttgaaaatca tgtcttcatt acctcggctt
    17581 tgtacggtgt tgttccagcc ttgtcaccca tggctcctca ccgtttggat tttttgatga
    17641 aattaaaagt cgctggtaag actttgaaga gccattggaa ggcagtctat gatgaagctc
    17701 tgaagaagga agaagtgatt ttctctctct tgtcatcaga gtttgagact gtattttcta
    17761 aggaaatcag agcaaagatg gtgaccttca aattcatgga ggatagaggc ggtcagctga
    17821 agattcactc aactatctcc aagaaagcgc gcggggcctt tctaacagct ttaatagaaa
    17881 atcaagtaca aactgtgggg gaagcacgtc gcttgaactt tgctggattt gtttaccgag
    17941 aagatttgtc acaaccacag ggattggttt ttgtaaagga agtatagaaa tcagataaga
    18001 gaaaagtcag cacgattatg ctgacttttt tgataactta taaagtattg gacaaatgaa
    18061 tctcatttga taaggtgtta agatgattaa ccaaattcat gattgacaga tgatttcggc
    18121 aaataaactt cttggtaatc cctgaaatat agaaattaga cacgatgata tcgtaatctg
    18181 tctgatttaa aatctcagga cttgttttta attcatccca aatatcgaag gtaaagcggt
    18241 tattacagta ataggaaagc atatcaacaa aggtaaggga tatagcatgg tcaaagttac
    18301 tgataatcaa aaccttgata ggtggtcgat tttctaacaa ctgagtagag aggttttcag
    18361 catgggttaa gatggtgtac atcaagtgtt ccaactgttc aggatggtca tgctgtccaa
    18421 ttgcctgacg gtattgagcc agttcttgac gcgcacttcc cataaagtct gggaagtaaa
    18481 cttcaaattt tttaatcgtc aatgcttttt gttcaaataa gattggggta gaaaagattt
    18541 cctgtctttc aaagaaagca gtgttgtgta aatgccaaat cagctcatcg tggttagtaa
    18601 aagtaatctt atattgtttt gataggagtt ctaatatttg actaagtaat tgataagagt
    18661 atcttgcttg actgttatct tctaaagagt tgaagaattc ttgaggatct aggaaaatat
    18721 cattctgaag aaaagaaata aaaatttgag cgattgtatc tggagtcatt tcaagcccaa
    18781 aatgtttaga gaaataagca agtgtctcct ggaaatttgg aatgctcttt aaaagaggat
    18841 aatattcttt gtaaaagtgg ttggggaggt caatgaagtg accattttta accctgtgga
    18901 tactgatagc tatcatcagt ttgtacattc tataaattga aaatcgcatt ggataattcg
    18961 tgattttata gaagaagtca gcaaactctg ttaaatcttc ctcaggaagg tcaggaaaag
    19021 gccaatctag gaaataatag cgctcagaaa agtattgggc aaaaaagtaa cgaatatcga
    19081 tctcatttcc acgtatctcg gatggagtaa aagataattc aatttgaaac tgagatgaaa
    19141 gaacctttgt aatatttctg cccagtcgat agaggttggc actactaaag taaagatttt
    19201 ctattgttcg ataaataggt agcccctcat tgaagaacat atactctagt aattgaaaag
    19261 attgagaatt agcaagaaaa tattgataaa tatcttctac actagtgtta acttctaaat
    19321 ctatcatgat tccgttaaca gaagattgga tgttaataga tggaaaggca atacgtaatt
    19381 catttaaatc actttttaag attcgttctg tacatcctaa tttttcggct agttcatgta
    19441 atttcatcca attacgattc tgaattaggg tttccattaa gcgtaattgt ctttgaactt
    19501 ttgtggataa aaggtttctc atacgggtat ttcctccata attcattata tctttttttg
    19561 tctaagtagt ataaaaaaat tagagcaaga aaattgtcgt tagtaatcgg aaaaattaga
    19621 ataatataag tggcttttaa gtgcagaaac agaatatatt aattgttcag aaaatattga
    19681 ttaatgcctc
//
LOCUS       DAVLSL010000024        18864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLSL010000024 DAVLSL010000000
VERSION     DAVLSL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.024"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(677..1903)
                     /locus_tag="VXN30_000681"
     CDS             complement(677..1903)
                     /locus_tag="VXN30_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1357345.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSHHLYITRVEGASLEERNLIIQEL
                     AKAGITSNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(1946..2638)
                     /locus_tag="VXN30_000682"
     CDS             complement(1946..2638)
                     /locus_tag="VXN30_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1357346.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALIL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(2811..4562)
                     /locus_tag="VXN30_000683"
     CDS             complement(2811..4562)
                     /locus_tag="VXN30_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357347.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(4552..6297)
                     /locus_tag="VXN30_000684"
     CDS             complement(4552..6297)
                     /locus_tag="VXN30_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357348.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(6425..6799)
                     /locus_tag="VXN30_000685"
     CDS             complement(6425..6799)
                     /locus_tag="VXN30_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1357349.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKP
                     GVLYSSKISSNRVNVALPYVGY"
     gene            complement(6952..7408)
                     /locus_tag="VXN30_000686"
                     /pseudo
     CDS             complement(6952..7408)
                     /locus_tag="VXN30_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            7542..8369
                     /gene="xth"
                     /locus_tag="VXN30_000687"
     CDS             7542..8369
                     /gene="xth"
                     /locus_tag="VXN30_000687"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1357350.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            8381..8524
                     /locus_tag="VXN30_000688"
     CDS             8381..8524
                     /locus_tag="VXN30_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1357351.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            8635..8763
                     /locus_tag="VXN30_000689"
     CDS             8635..8763
                     /locus_tag="VXN30_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV1357352.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHEFAVLDPNGYFLRFSE"
     regulatory      9146..9241
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            9641..10222
                     /locus_tag="VXN30_000690"
     CDS             9641..10222
                     /locus_tag="VXN30_000690"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1357353.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            10222..11484
                     /locus_tag="VXN30_000691"
     CDS             10222..11484
                     /locus_tag="VXN30_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1357354.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIAMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(11535..11996)
                     /locus_tag="VXN30_000692"
     CDS             complement(11535..11996)
                     /locus_tag="VXN30_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1357355.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(11993..12757)
                     /locus_tag="VXN30_000693"
     CDS             complement(11993..12757)
                     /locus_tag="VXN30_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1357356.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNDPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(12938..13342)
                     /locus_tag="VXN30_000694"
     CDS             complement(12938..13342)
                     /locus_tag="VXN30_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1357357.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(13412..14893)
                     /locus_tag="VXN30_000695"
     CDS             complement(13412..14893)
                     /locus_tag="VXN30_000695"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1357358.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(14912..16090)
                     /locus_tag="VXN30_000696"
     CDS             complement(14912..16090)
                     /locus_tag="VXN30_000696"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1357359.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPATISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVSLDIQTLGELDEWAVDQYSYLIKDENRLKRVR
                     HAVLENQRTLKAQVALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            16254..17261
                     /gene="galR"
                     /locus_tag="VXN30_000697"
     CDS             16254..17261
                     /gene="galR"
                     /locus_tag="VXN30_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1357360.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTKLTLRESTLNQ
                     E"
     gene            complement(17308..18345)
                     /locus_tag="VXN30_000698"
     CDS             complement(17308..18345)
                     /locus_tag="VXN30_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1357361.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(18346..18699)
                     /locus_tag="VXN30_000699"
     CDS             complement(18346..18699)
                     /locus_tag="VXN30_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1357362.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
BASE COUNT         5517 a         4280 c         3658 g         5409 t
ORIGIN      
        1 aacaaatacc ttgtcataca agcattcgcc agggatattt acctgaatat cgctcatatg
       61 gcggattgaa actaaaacac tacaatatat ttcttaaata gaccttctat aaaagctaga
      121 tttaacctcg ataaaaatag atgcagtgct tctaaaatac tggaataggg catataggta
      181 atcgtagaca agtgttttat agattgtctc gcaagagatc taatgatgta tagcaagcat
      241 cactaaacat atagaatatt tgtattatta taatatcaac atgctataaa ttctatcatg
      301 caaaagaatg actagaacat gaaagacagg cacaaaactt tgaacaatat tctgaaaaca
      361 aaaaaagacg cgagtttcca caatctcact catggaaact cacgtctttc aaaaaatagg
      421 gtgaattgaa aactagaaca agacactaga ttttctaaat gatgtctagt aaggattcta
      481 aatgctacac caaattgctc acatcttatt tcaaaccacg ggactcccac aagaaaggaa
      541 aatcctataa aaattctctc tctgaggaga aaggagcctc aactacaggt gtaaactatt
      601 atcaattgaa aacaacagtc tttacactca attagacttt ttaaggacag gatcactttc
      661 gcttgactgt agtttgtcat ttttttgata aagttagcac tttttcagaa actgttttga
      721 aagtctcaat gatatagtct acttcttcat cgcttaattt agtatgaaga gggagggtaa
      781 tttcattctc aaagaaggca taggccttag gatagttcgt catatcaaat ccaagattct
      841 tataggctgt caagagagga agcggtttgt agtgaacatt acttgtaatt cctgctttag
      901 ccaattcttg gatgatgagg ttgcgttctt ctaggcttgc tccttctaca cgggtgatgt
      961 agaggtggtg tgaagattcg acagtttcag tcttgtgtgc caaaggatgg atgcgagaac
     1021 ctgcaaaacc actatcatag cggtccacaa tgtccttacg gcgttgcaac aaacttggat
     1081 agcggtccaa ttgtaccaaa ccaagtgaag ccatgatatc ggtcatgttg cacttatagg
     1141 ctggtgtaac gatatcgtat tcccatgacc ccagttgcat cttggcaaga gcatccttag
     1201 tttgcccgtg aagggaaagg atttggaatt ccttgtacat ctcttcgtca tcaatcactg
     1261 gattggcttt ccaagtcgca cttccacctt ctgccgttgt aaagttctta acggcatgga
     1321 atgagaagga agtaaagtca gcgatagaac cagaaggttg tcctttataa gtagatccca
     1381 aagcgtgggc actatcagag acaatgacaa tacggttaaa ggccttttgc cacttgcttg
     1441 aagcggtaaa gaagtcacgt tttttctcca cgacttggaa caaacggtca taatcgcaaa
     1501 caatccctgc gagctctact gggataatca ccttagtttt ctcagtgata gcttgctcaa
     1561 gcaggtcata gtccatctca aacgtatctg cttggatatc caccatgaca ggggttgctc
     1621 ccacgtgcgt aatgacacta catgaagccg tataggtcat ggctggaacg atgacttcat
     1681 caccaggtcc cacttccaaa acgcgtaaaa tcaactccag agcggctgtc gcagagttga
     1741 gacaaacagt cttaggtgtc tgggtgtaaa gagacaagcg gcgctccagt tcttttgttt
     1801 taggacctgt tgtgatccaa ccagaacgca gggtatccgc tacttcagca atttctgctt
     1861 ctgtgatatc aggcggtgaa aatggaatat tgtaatttgg cattgatttg ctccttttat
     1921 ctgtactcat tttcttatga ctactttatt ttagtacctc aaacacggtt tgaaacatga
     1981 ttttgatgtc cccaaagaaa ctaaactctc ggagataggc gaggttatag cgcatctttt
     2041 caggaagaac atgctccaca taggcctgat caactgacag acctttctcc gtcatttgac
     2101 tgatgatggt gtcctcatcc ttgtagttga tgctggctgg agaggtaatt cctgcttgca
     2161 agagcaaggt tgccatcatt tcagggctat actgctctgt ataacgtggc acttcaggtc
     2221 gtgtaccgac aaaggacatc tcacctttaa ggacattgac caactgaggc agttcgtcca
     2281 aacggacacg tcggatgaaa tttccaacct tggtaatacg gctatcgtta gcagaagtca
     2341 ccagacttcc ttttttatcc gcatccgtca ccatggtacg aaacttccaa atcttgaacc
     2401 gacggttgta ctgggtcaca cgctcttgct tgtaaatcac tggccccttg ctatccaact
     2461 tgatccaaat gctcaagatg agaaagatgg gagaggtcag aaccagtaag atcaaggcca
     2521 aaacccagtc caagcaacgc ttgaaaatca gcgaaccctt ccttttagag acaagctggt
     2581 agtaagactc aacctcgctt gatttcattt ccacaggcaa gtcttcccat ttcagcatgc
     2641 tgttctcctt tgtttttatt atactatttg tcaacatttt tcattatacc acaaaatgga
     2701 aaaggcggtg aaagaaatct gtaatttgaa ggatttcctt ctagtactca atgaaaatca
     2761 aagtacagct ttgagattgc agataaagct gacatggttt aaagagattt tcaaagagta
     2821 tcggccatgg cccctgcctg caaactatac atcttgtgat aggttcctcc cagagccaag
     2881 agttcctcat gggttccact ctcgataatg cgtcccttat ccaagacata gatgcagttg
     2941 gcgtcttgaa tagtagaaag gcggtgagcg atagcaatag ttgttcggcc ctgtctcatc
     3001 ttcgccagag aagcttgaac caagctttct gtttcagagt caatattggc tgtcgcttca
     3061 tccaaaatca ggattttagg ctggctggcg actgttctag caaaggcaag aagctggcgc
     3121 tgcccagtag agaagctcga accacgctcg gaaacagggg agtcgtaccc ctgaggaagt
     3181 tcttgaataa aggaatctgc atccacaaag gctgccgcag cctgaacctg ctcatcactg
     3241 atttcttggt acatggcgat attggactta atagttccat gatagaggaa gggttcctgt
     3301 aaaaccaaac cgatgttttt tctcagctct tcttgactga aatccctgat atccacatca
     3361 tccaagagaa ctctccctga ctggaattca taaaagcgca tgaggacatt gataatagac
     3421 gatttccctg aacctgtatg acctacaaag gcaatggttt cacccttatt aacagagaaa
     3481 gaaatgtcat ccagaatcgg atgtttaccg tcatacgaga aacacacatg ttcaaaacgg
     3541 atattgcctt ctttgacttt ggcttgccca ttttcttgaa ggggctcata ggtcctctca
     3601 tcgattaagg caaagacacg gcctgcagaa accatagacg tttgcagtgt tgaaaagttt
     3661 tgcgtcacct caatcaaagg gtcaaagagg cggttgatgt actggataaa agcatacatg
     3721 gtcccggccg ttatcccgat agaaagacca cgatagccaa aataggtcat caagacagca
     3781 taacctagaa gtttcaacaa actcatggca ggtctcaaaa agagggcatc caaggctaca
     3841 gaacggttgg cataggccaa gtgctcttgg ttgatttcat caaattctgc ctgcaggcgc
     3901 ttctcttgat taaaagcctg aataatcctg attccctcga tattctctgc cagcttacta
     3961 ttgatatctg acaagagact tctggttttc tcgatgattt tcactgactt ttttcgatag
     4021 agattgacca aaaggaaaat caaggggaga aagagcaaga ctaatgctgt caaacgaaaa
     4081 tccagcacca acatggtata aagggttgtc agaaagataa aaactgctga gataaagctg
     4141 gataaaatcc cagaaaacat atcactaatc gtctcggtat cgttggtcaa acgagaaacg
     4201 atagaacctg ctggcgtctt gtcaaagtaa gacatgccca gtttctccat attggcaaag
     4261 gcatcccgac gaatatccct aacaatactg taagacacgc gcgcaaagag aagattgccg
     4321 acatactgaa ctacagtttg taggatgtag agaccatagt agaccagcaa aacggtcacg
     4381 gctagttggt taagattgct gagatactgg tcgataaagt gggaagccac aaggggaatg
     4441 acacttttaa tgaccgtcgt cgctaggaga aaactgagtg ccaaaaaggt aaggagtcca
     4501 taaggcttga gataggacat caagcgcttc aatacagtcc attgttcttg tttattctgc
     4561 atcttcttct cccttcattt ccagctgctg agactggtag gtttgggcat accagccatc
     4621 caaagctagc aagtcttcgt gcctgcctcg ttcgataatt tgaccatttt gcaggactaa
     4681 aatcaaatct gcatggacaa ctgcactcag gcgatgggca gtgataatgg ttgtcttgtc
     4741 ctttcgcgtc tccttgaggt tgtcgataat cgcatactct gtcttggcat ctacggcgga
     4801 taaggaatca tccaaaatca agatatcagg gtctaaaatc atagcccgac tcatagccaa
     4861 ccgttgcttt tgaccaccag aaagactgac tcctttttca ccaatcagcg tatcaaatcc
     4921 ttgaggcatg tctacaatat cttggtaaac tcgggctagc ttagtagctt cctcgaccgc
     4981 tgaaaggggc aagttaggat tgccaaagcg gatattgtct aggattgaag tcgcaaaaag
     5041 aaactggtcc tgaggaacat agcccatgag actgcgaagg tctgtcagac gatagtcccg
     5101 aatatcgtga ccgtttagat aaatggcgcc cttatccaca tcgtattcac gcaagaggag
     5161 cttgattaag gacgttttcc cagagcctgt ctgcccaacc aagcccagtg tttgcccttt
     5221 tgccaaacta aagtgaatat ccgtcagtgt ttcctcattt tcaaaagcaa agctgtcaat
     5281 ggcatactcc aaacgcccat tttcaatacc atccagagga aactccgggt cttgtacagg
     5341 agattcctga gacaaaagat tttcaatccg ctggtaagaa accttccctc gctgagtagt
     5401 attaaagagg aagccgatgg ccataagagg ccagaccagc atatccaaat agctgataaa
     5461 ggtgactaga ttcccaactg taatctgccc ttcctgaacc accaaggagc caaccaaaag
     5521 cgttaaaaca taggacgaac caacaaacaa gagaaccata gggtcaaaga gactatcata
     5581 tttcatggtt tgcaggttct tttggaaggt taattcattg actgcctgaa aagacttcaa
     5641 ctcgtctgcc tgataaccga aagacttggt cactttgata cctgatacgg actcctgtac
     5701 cttgttattg agttcagaaa aagcagcttg ggattcgcca aaggccttat gagtctttct
     5761 ccctaggcga ctagtcgcat aggccatgaa aggtaggggg agaatggcaa caagagtcat
     5821 ttgccatgag atgctaaaga gcatggtcaa caaagtcacc agagccgtga tagaggcatc
     5881 caccgcagac atgacaccgc cacctgctaa acgagtcaag gcattgatat cattggttgc
     5941 gtgtgccatc agatcacccg tccgataggt ttgataaaag gctgacgaca tttttgtgaa
     6001 atgcttaaac aagcgagacc gcatgatctg tcccaagcaa taagaggtcc caaggatata
     6061 catacgccac acatagcgca aatagtacat accaaaggct gcaagtagca agtaaaatag
     6121 gctaagaagg aggtcctgct gggttaattg ccccgatgtg atggcatcaa taacccgccc
     6181 cataaccata ggaggaatga gattgaggac ggaaaccaag accagggcca caattccgac
     6241 tagataacgg cgtttttcta acttgaaaaa ccaccaaagt ttttgaataa tggacataaa
     6301 atccctttct gattgcaaat ggaaacctga ggccaatact caatgaaaat caaagagcaa
     6361 actaggaaac tagccgcagg ctgctcaaag cactgctttg aggttgtaga tagaactgac
     6421 gaagtcagta acctacatac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag
     6481 agtacaagac cccaggtttt tctttttcat aagttacgac tgtaacagca cccttgtcat
     6541 attcagcaat aaagatattg gctacattgt catgcccttg tttactgagg ttatcaagca
     6601 accactcttc gctacgacca atcgattcca agacgtctac ttgaatcaca ccgtcagtca
     6661 caactggata cttaggattt tcatctccca tttgcacaac gatgagttgc ccattttgct
     6721 cttgcacagc tcgtttgact tgcttcatct ggaaaatccc ttggctacga agtttgaggg
     6781 ctacatccga tgcagacaaa ccaactgaac gacaggctta tgggtcaata ttcccatttt
     6841 tgataaggag agttggctta ccatcaatca aacgtttcac aaagtgaaca ttgttgttga
     6901 gctatttgag agtcaagatc agtttgatta aaatttcgat ataattaagt gtcatttgtt
     6961 cacatccacc agttcaattg cgtctgtctt atacaattcg actttctcta acaggtactt
     7021 gtctggctca cttccgttta gggcacgata atagcgatct cccaccttga taagtgcgcc
     7081 atctgtgttt gtggaagtat taacatagac ttctgacttg tctactccca aatctttgga
     7141 aacaacctcg atgaaatgaa gtaaagtttg aaatttattg ttagaaactt gattgttttg
     7201 ataagtcgtg atgctgacca aaagcagagc taaacaaggc cagagctgaa atcatgacca
     7261 actcacgaaa tttgattcct ttttttatca tggtatgcct tgaaagcaaa aaaactgtca
     7321 cagctaataa aacaatccca aagccaacca tgataccatt ttgctgaccg atttggctga
     7381 gtacatagtc ataagagtag aatttcatgt attttcactc cctttcataa aatcattctt
     7441 aattataaac gaaagagagt aatttttcaa accatacgtt ggggaaatag accttttttt
     7501 ggtataataa aatctataat ctgaatgaaa aaggtaactt tatgaaactt atctcatgga
     7561 atattgattc cctaaacgct gccctaacta gtgactcagc tcgtgccaaa ttgtcccaag
     7621 aagtcctaca aaccttggtc gctgaaaatg ctgatattat tgctatccaa gaaaccaagc
     7681 tttctgccaa aggacctaca aagaaacacg tggaaatttt agaagaactc ttcccaggct
     7741 acgaaaacac gtggcgctct tcccaagagc ctgcccgtaa aggctatgct ggaaccatgt
     7801 tcctttataa gaaagaactt acgcctacga tcagcttccc agaaatcggt gccccttcta
     7861 ccatggactt ggaaggccgt atcatcactc tagaatttga tgcatttttc gtaacccaag
     7921 tttacactcc aaacgctggt gacggtctca aacgcttgga agaacgccaa gtctgggatg
     7981 tcaaatatgc ggagtatttg gctgaactag acaaagaaaa accagtcctt gcgaccggtg
     8041 actacaacgt agcccacaat gaaatcgacc ttgcaaatcc tgctagcaac cgccgttcac
     8101 ctggatttac tgacgaggaa cgtgctggat ttaccaacct attggcaact ggatttactg
     8161 ataccttccg ccatgttcat ggcgatgttc ctgaacgcta cacttggtgg gcacaacgca
     8221 gcaaaacttc taaaatcaac aatacaggct ggagaatcga ctactggctc acaagtaacc
     8281 gtatcgctga caaggtcact aaatctgata tgattgactc aggtgctcgc caagaccata
     8341 caccgattgt cttggaaatt gacttgtaag gattttctat atggactaca atgcggtcat
     8401 tcccgagttg cttgtatcga atatcgaaca gtcccggtcc ttctactgtg gtttactggg
     8461 ttttaggatt gaataccaac gtccagaaga aaactttctc tttctcttga agagtgtcaa
     8521 ctaatgttag aggagggaac aaaggatcaa ctagctgaac tgacctatcc atttggtcgt
     8581 ggagtcaatc tctcctttgg cataaaggat gtttcaaaac tctattaaag agtgatgaag
     8641 gcaaactatc ctatttatcg tcccttgacc aagagaaagt ttcgtgtcag tgatccctat
     8701 atctatcctc atgaatttgc agttttggat ccaaatggct attttttaag atttagcgag
     8761 tagaataata aaaattggga aaagtcttta aagttagaaa aaggcatatt gtcaggtgtc
     8821 taaaaacttg atagtatgcc ttttatgatg gaaaaatctt ctaatactct tcgaaaatct
     8881 cttcaagcca cgtcaacgtc gccttaccgt aggtatatga tactgactct gtcaattcta
     8941 tccacaacct caaaacagtg ttttgaggtt gtggatagct tcctagtttg ctctttgatt
     9001 ttcatttagt ataaatgtta gcttacaatg gagtttttgt tcaccctctc ttctttttgt
     9061 tcgcttttta aaccaaaaca ctaacaaaat agttgatttc taaagttatt tagtgtaaaa
     9121 taaaaacatt ggctcaagcc tatttaaaat tgaatatcgt tttacttgtt tatgtcgtga
     9181 attggcacga cgtttctaca aggtgccgga acacctaaca ataagtaagt cagcagtgag
     9241 atatggccgt ttttgccatg tctatttagg ggacttactt agagattaag taggtctttt
     9301 ttgaatattt ttaatatttc ccttaattag tgcggacggg aggtgaaatt atcctgtgaa
     9361 taatttcagg aatccaggga atttcatccc taatttttga gcccctagct ctttcatttc
     9421 taggcttagg ctaaaatagt tccccgaact attttactct caaaaatccc gtcatagaca
     9481 ataacaagat tattgtctat gataccacta caacgggaaa tatttgataa gactcgcttc
     9541 gctcgtaata gttttatcaa atatatagta ttagcaaaag ggatcactat agaaatattt
     9601 aaacaacaag taaattaact tttagacttt aggaggtctt atgaaattat tagaagagcg
     9661 catcctcaag gatgggcata tcttgggtga taacatcctc aaggtagatt cctttttaac
     9721 ccaccaagtt gactttagct tgatgcgaga gattggtaag gtttttgcgg aaaaatttgc
     9781 tgctgctggc attaccaagg tcgtaaccat tgaagcgtcg ggtattgccc cagccgtttt
     9841 tacagctgaa gccttaaacg ttcccatgat tttcgccaaa aaagctaaga acatcaccat
     9901 gaatgaaggc atcttaactg ctcaagtcta ctcctttacc aaacaggtga ccagcaccgt
     9961 ttctatcgct ggaaaattcc tctcaccaga ggacaaggtt ttgattatcg acgatttcct
    10021 tgctaatggc caagctgcta aaggcttgat tcaaatcatc gaacaggccg gtgccacagt
    10081 ccaagctatc ggtatcgtga ttgagaaatc cttccaagat ggtcgtgatt tgcttgaaaa
    10141 agcaggctac cctgtcctat cacttgctcg cttggatcgt tttgaaaatg gtcaggtcgt
    10201 atttaaggag gcagatctct aatgcaaact caagaaaaac actcgcaagc agccgttctg
    10261 ggcttgcagc acttactagc catgtactca ggatctatcc tggttcccat catgattgcg
    10321 acagcccttg gctattcagc tgagcagttg acctacctga tttctacaga tatcttcatg
    10381 tgtggggtgg caaccttcct ccaactccaa ctcaacaaat actttgggat tggactccca
    10441 gtcgttcttg gagttgcatt ccagtcggtc gctcccttga ttatgattgg gcaaagccat
    10501 ggtagtggcg ctatgtttgg tgcccttatc gcatctggga tttacgtggt tcttgtttca
    10561 ggcatcttct caaaagtagc caatctcttc ccatctatcg taacaggatc tgttattacc
    10621 acgattggtt taaccttgat ccctgtcgct attggaaata tgggaaataa cgttccagag
    10681 ccaactggtc aaagtctctt gctttcagct attactgttc tgattatcct cttgatcaac
    10741 atctttacca aaggatttat caagtctatc tctattttga ttggtctggt tgttggaact
    10801 gccattgctg ctactatggg cttggtggac ttctctcctg ttgcggtagc tccacttgtc
    10861 catgtcccaa ctccactcta ctttgggatg ccaacctttg aaatctcatc tattgccatg
    10921 atgtgtatca tcgcaacggt gtctatggtt gagtcaactg gtgtttatct agccttgtct
    10981 gatatcacaa aagatccaat cgacagcacg cgccttcgca acggttaccg cgcagaaggt
    11041 ttggcggtac ttctcggagg aatctttaac accttccctt acaccggatt ttcacaaaac
    11101 gttggtttgg ttaaattgtc aggcatcaaa aaacgcctgc caatctacta cgcagctggt
    11161 ttcctggttc tccttggact gcttcctaag tttggtgccc ttgcccaaat cattccaagc
    11221 tccgtcctcg gcggtgccat gctggtgatg tttggttttg tatctattca agggatgcaa
    11281 atcctcgccc gagttgactt tgctaacaat gaacacaact tccttatcgc agctgtttca
    11341 atcgctgcag gtgtcggtct caacaacagt aatctctttg tcagcatgcc gacagccttc
    11401 caaatgttct tctcaaacgg aatcgtcgta gccagcctac tcgctattgt cctcaatgcc
    11461 gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt gtgtgacaaa
    11521 ataaaaacat ctacctattc atatatgcta gtgtcatagt cctgaaaatc atctgcatca
    11581 agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc atctggattt
    11641 tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt atactttttc
    11701 agttttagat tgctttccaa taattcacct attaacaata atctatgata ggagctatgt
    11761 agccactctg gcaatttatc tcctacttcc cacccatatt tttccttcat caaagctagt
    11821 tgtttttcgt caccaaattt catattttca gataaccatc tcacacaatc gggatctgat
    11881 aaggtacaga cgattaaact taggttatcg taacggcttt cagtttcata atcaggccag
    11941 atgactttta agaaatcata cacctcttct gatgcaacaa gttgtttcgt tttcataatt
    12001 tccgatactt tcctctacct ttaaattcta ttatttcttt gtctcttaat atttgaagct
    12061 gttgcctaat cttttctttg atatgattgt tcttaacaaa gatatttttt aggtcacttt
    12121 caaaacgata catatcttca agggtaaatt ctgaaccctc tatcttatct atacaattta
    12181 gaatttctat tgtccaacct cttgatgaca gagagctctt ccttaaaaat aaaccttgct
    12241 taaattcttt tgtaactttt tctggatctc taacttgtcc atcttgcaca agaaatatcc
    12301 ttcctttaga aggtacttgt gataaatcaa tgttacaacc aatccaacct gctcgtctag
    12361 cagttggtgc aagtggtttt ctttgaataa tcgatttcgg tgtaacaaat tgcttcggaa
    12421 ggacaagaaa gttatttacc tcaaaatttt ttgtgtaagt taaaaaaaag aaattaggat
    12481 tattatctgc ctgcacacgc ttcatcatcg ttgcataagc accatcattg attgttgatg
    12541 aaaaatttcc ttttttgctc tttagttcaa actcctcact acaatgatta cagtaaaaat
    12601 ctgctacagg ccgattattt tcaaaatgat ttaagggatc gttcccacaa ttagggcaat
    12661 aagactgacg ataaacccaa tcctccgtta agatacgtgc cttttggcta ttacttttat
    12721 aggtttctac taattctaaa ttaaagtgta attccattta aaaattcctc actaatatga
    12781 gaactcactc gatataagca attaaaatct actataacta gtataccaca tatatgtacc
    12841 tattacattt gtatttcatc actctctttt atatttcctt ctaacatatc tcataaatta
    12901 aaaatgagtg agataaattt atcccactca cctttcttta tatacttacc cgtctttcct
    12961 cagacctttg gcctgtgaca aacatggtaa aggttgcctt acagacattt ctgccctctt
    13021 gattggtgat atccacatcc atcacacagg ttgtacgacc ttgatggaca cattctcctt
    13081 taatggtcaa tacatcgtcg agttttcctg ccttaaggta gttgatagag gattggagtg
    13141 tcaccccatc aagtcccagc gagataacca ccaaaccact gatttggtca catagcgtga
    13201 atagatagcc accatgggca ttgccatagt agttgagcga tgagttcact actttggtcg
    13261 tcaccacaac gtgaccatct ctcatttttt caatttcgta attttcaaag gcagatatag
    13321 cgtcaaaatg aaagtctttc atcgtttcct cctgatattt caaacgacac tatgatacta
    13381 ctttttatag gactgtgcaa ggagaaagct cctagtctag taaaattccg acctgttcca
    13441 caaagcgcat aaaggctgtc tgcccttgtt ctgtctgctt gtagactcct gcatcctcaa
    13501 gtacacgcac aaagatagca cccacagagt ccttgacgat tgcaagggct ttttctttat
    13561 ccgttaggtc tggatgttgg gatttgagtt ggtctgccca ctcctgatga taatcggcaa
    13621 ctgtaacagc ttctcctaca agatagctag cgacttgctc tacttcttct ttcagacgtg
    13681 gtggcaagat tgccaagccc atgacctcaa tcaagccgat attttccttc ttgatatgtt
    13741 ggacatcctt gtggggatga tagatgccat caggatgctc tgctgaagtc tgattgtctc
    13801 gcaagaccaa gtccaactca aactgtccat cacgcttacg cgctattggt gtgatggtgt
    13861 gatgcggtgt cccgtctgtc tctgccaaaa tctgcacact agaatctgaa tactgacgcc
    13921 attcctggaa aatcttatca gctaagttaa tcaaatcctc tttggaatcc gaagtcaagc
    13981 gtaggacaga cattggccac ttgataattc cagccttgac ctgctcaaaa ccagcaaatc
    14041 ggaaggtctt ttgcaaggga gccaattcca taggaaatac gtgacggcct ccctgataat
    14101 gatcatgagt tagaatagag ccccccacaa tcggcaggtc ggcattagat ccagcaaaat
    14161 atcctggaaa ctgctctacg atagccaaca gacgttcaaa actctgacga ctaatggcca
    14221 tgggacgatg ctggccatct aaaaagatac aatgctcatt aaagtaagca tagggcgaat
    14281 actggaaccc ccattcctga ccaaccattt caaaacggat aatccggtgg ttgctgcgag
    14341 ctgggtggtt gacccgacca tggtaaccct cattctctaa acaaagttga cactgaggat
    14401 aattactagc ttgcaccaac ttggctaccg caatctcttt cggatccttt tcaggcttag
    14461 agagattgat ggtaatttca agttctccat aatcagatgg aacacgataa gcgatatttt
    14521 tagcaatggc cttgagtttg atgtaatcat ttttctgact gagttgataa aaatcctcta
    14581 tcgcttgctc aggagagtgg acataggctt cccagaagtc acgattgacc tgactcggat
    14641 aaggagtcac caagtccatc agttcagtac caaggatttc acgcgcagtc tgactatcct
    14701 caatcgtctc taatcgaacg gcttcttcaa ccagctggtc cttgaggtca atcaatttat
    14761 ccagattggt ctcaacttcc aaaacacctt ctcccactcg tgccaaaaca cgattggtca
    14821 gatagattcg atccatttcc tcaaatgagc tttcagaaat gacatgtgtt acaaatttat
    14881 ctactaaggt cactatagag cctccttttg actagtcaag gacgcgagtg ccacctgcaa
    14941 cttcagcgat atagaagctt ggagcgtatc caactacttc ctcgtagtgt ttgcctacag
    15001 cttccttaaa ggcctcaaca gtatcttttt gaaccaaggc aatggcacag ccaccaaaac
    15061 cagcccctgt catacgagca ccgagaactc cttcttgtgc ccaagctgtg tgaacaaggg
    15121 tatccaattc caaaccagtt acttcataat catgctccag agaaacgtgt gacgcattca
    15181 tcaagcgtcc aaatgtttcc aaatctcctg cttggagtgc tacttgagct ttgagggtac
    15241 gttggttttc aagcacagca tggcgaacac gtttcaaacg attttcatct ttaatcagat
    15301 agctatattg gtcaacggcc cactcgtcca attcacccag agtctgaata tccaaggaaa
    15361 cttgcaattc ttccactgct ttttcacact cagcacgacg ttcattgtat ttagaatccg
    15421 ccaattcacg gcgtttgttg gtgttcatga taacaacgac attgtccttc aaatcaagtg
    15481 gcaccaaatc atactctaag gtgttggtat caaggtaaat agcacgttga tcagccccca
    15541 taccaatagc aaactggtcc atgataccag agttgactcc gataaagtta ttttctgttt
    15601 gttttccgat tttaaccaaa tccaaacgct ctaattttaa atcaaagaga tgctctgcca
    15661 ctacccctgt caagagctcc aaggatgctg aagaagacaa accagcacca tttgggatat
    15721 tcccataaac ataaaaatca aaacctttgt caatcacgtg cccagcttct tgcaagaaat
    15781 gaaggacacc ttttggataa ttggtccagt tgtgctcttt ttcaaacttg aggtcagcga
    15841 gaggcacttc gataatgccc ttgtcctcaa agttagctga gtagaaacgc aaaacttggt
    15901 cgtcacgctt acgagctgca ccgtaagttc ccaaggaaat agtagcagga aaaacgtgcc
    15961 caccgttgta gtctgtgtgt tcaccaatca aattaatacg gcctggtgaa aagaaagttt
    16021 ggtctgcttc ttgaccaaaa acagcaagaa agtctttgcg aagagtttca gcagtaagat
    16081 gttgtgtcat atgatttctc ctttgactgt ccgttaagcc accttaacgt gtaatcgttt
    16141 tcttctattg tatccaagga atttcccaag gtcaatcctt tttactaaaa ttttagtaaa
    16201 ctatcagtaa aaagaagaaa aatatgatat actaacctaa tcaaggagga tttatggcta
    16261 ccttaaaaga cattgcacag ctagcctctg tctctatcgc gaccgtatcc cgcgtcctca
    16321 accgcgacca gagcctatct gttacagaag aaaccagaca ccgtatttta accgttgctg
    16381 aagagctggg ctacaccaag cacctcaaga caggcgagtc ccataaaccc aagcaaaaga
    16441 ttgccattat tcaatgggtc agcgaacaag gggagctgga cgacctctac tactaccaga
    16501 ttcgcctagg aatagaaaaa agagcccaag agttggacta tgatatcttg cgctatttta
    16561 atgaccatcc ttttacctta agcgaggaag tgattgggat tctctgcatc ggaaagttta
    16621 gtcgagctca gatttctgcc tttgaagaat accaaaagcc tcttgtattt ctagacagcg
    16681 atacactttc cctgggacat acctgtatta tcacggattt ttacactgct atgaaacagg
    16741 ttgtcgatta tttcctcagc caaggaatgg accgtatcgg gattctaaca ggccttgaag
    16801 aaacaactga ccaagaagaa atcattgagg ataagcggct ggaaaatttc agaaactaca
    16861 gtcaagcaaa aggaatctat catgatgaac tgatctttca aggaaacttt actgcccagt
    16921 ctggctatga cttgatgaag gaagccattc agaacttggg agaccaactc ccaccagcat
    16981 ttttcgcagc cagcgatagt ttagccatcg gtgcccttcg tgcgctccaa gaagctggaa
    17041 tcagcctacc agaccgtgtc agcctcattt cctttaacga caccagcctg accaagcagg
    17101 tttatcctcc cctctccagc atcacagttt atactgaaga aatggggcga gcaggtatgg
    17161 atattcttaa caaggaagtc ctccacggtc ggaaaatccc tagcctgacc atgttgggaa
    17221 ccaaactgac attaagagaa agtaccctaa atcaagaata ggataacata aaaaacgaat
    17281 aggagtttta gaactcctat tcgtttttta ttcgattata atcatagact taatagtctt
    17341 acgttcatcc atatctttgt aggcttggtc gatatcttcc agtttataac ttgaagtaaa
    17401 gacgcgacct ggattgatat caccatcaag gacggctttt agtaaaaatt gcttatcgta
    17461 tgttgtagca gaagctgccc cacctgctac agagatattt tgcataaatg tcgaaccaag
    17521 agcacgatta ttatagtgtg ggactcctac aaagcccata cgccctccat tatgaagaac
    17581 acctagcgcc tgttctatag cagcctccgt accaacacat tcaagtgctg catctgctcc
    17641 tccaccgagg atttcacgca ccttggtaat tccttcttga ccacgttctg caacaacagc
    17701 tgtcgcacct gactccatag ccatcttttg acggtcttca tgacggctca taaggataat
    17761 ttgtgatgct ccacgcatct tagccgcgat gacagcacat tgaccaacag ccccatcacc
    17821 gataacaaca accttgtccc ctttttgaac atttgcaaca cgcgccgcat gatagcctgt
    17881 cggcatgaca tctgcaagag tcaaaaggga cttgagcatc ccttctgtat agtcagaagg
    17941 ttgaccaggg attttaacca gcgcccagtt tgcatagtgg aagcgaatat attctgcctg
    18001 aaaatcaccc cccaaattat tgccaatatg attgtcgcaa gaaccgtcaa atccagcaag
    18061 acaggcatca cactcaccac atccatgtgt aaaagggaca atcacaaaat cacctggttt
    18121 caccgtcgta atggcttccc cagcttcttc aacaatccca atcgcttcgt gtccactatt
    18181 tttgtgtcca gctttcgttt ctggattacg gtacctccat aaatctgaac cacaaacgca
    18241 cgcacgaacc acacgaataa tcacatcatc cgcttctatt atttgcggac gttcaatgct
    18301 tgcaagtcca acctgacctg cctttgtata tactgctgat ttcatttaaa attttccttc
    18361 cttataaagt ttaattttga gatttaaacg atttaaagct gtctgtagct gagacaagcg
    18421 ctcctctaat ttttgctttt cctcttctaa aataccaagc ctctcctctc tcgtttcatc
    18481 tcccttttgg tagagcgaca tatagtcaac taaactatct acagagacac ccgccgaacg
    18541 aaaacactta ataaattcca gcgcttcgat atcctgatct tgaaaatcac gaatcccagt
    18601 agcagtacga gtaatcggtg gcacaagacc aacccgttca taataccgaa tcgtatccgc
    18661 tgaaattccc aacaaatcac tggcagattt aatattcaca cgctaacctc ctctcatttc
    18721 attcactgaa ttcacgctat tctaactcac ttatcttatt atataacttt gagttgactc
    18781 caagtcaagc attttgtatc taatcgaaaa atttttatca ttaacaatca tatatctgga
    18841 gtttatacaa tgctcttctc ttac
//
LOCUS       DAVLSL010000025        18686 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLSL010000025 DAVLSL010000000
VERSION     DAVLSL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.025"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(257..1063)
                     /gene="yidA"
                     /locus_tag="VXN30_000700"
     CDS             complement(257..1063)
                     /gene="yidA"
                     /locus_tag="VXN30_000700"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1357363.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMVGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1076..2380)
                     /locus_tag="VXN30_000701"
     CDS             complement(1076..2380)
                     /locus_tag="VXN30_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1357364.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSLIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2452..2829
                     /locus_tag="VXN30_000702"
     CDS             2452..2829
                     /locus_tag="VXN30_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1357365.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDRQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            2919..3251
                     /locus_tag="VXN30_000703"
     CDS             2919..3251
                     /locus_tag="VXN30_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1357366.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3263..4834
                     /gene="ffh"
                     /locus_tag="VXN30_000704"
     CDS             3263..4834
                     /gene="ffh"
                     /locus_tag="VXN30_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1357367.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5029..6312)
                     /locus_tag="VXN30_000705"
     CDS             complement(5029..6312)
                     /locus_tag="VXN30_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1357368.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6615..7328)
                     /gene="rsmG"
                     /locus_tag="VXN30_000706"
     CDS             complement(6615..7328)
                     /gene="rsmG"
                     /locus_tag="VXN30_000706"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1357369.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7422..7982
                     /locus_tag="VXN30_000707"
     CDS             7422..7982
                     /locus_tag="VXN30_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1357370.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7984..8883
                     /gene="htpX"
                     /locus_tag="VXN30_000708"
     CDS             7984..8883
                     /gene="htpX"
                     /locus_tag="VXN30_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1357371.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8927..10486)
                     /locus_tag="VXN30_000709"
     CDS             complement(8927..10486)
                     /locus_tag="VXN30_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1357372.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEISNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10725..11042)
                     /locus_tag="VXN30_000710"
     CDS             complement(10725..11042)
                     /locus_tag="VXN30_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1357373.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNNQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FEF"
     gene            11211..11753
                     /locus_tag="VXN30_000711"
     CDS             11211..11753
                     /locus_tag="VXN30_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1357374.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVGDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11753..12382
                     /gene="nth"
                     /locus_tag="VXN30_000712"
     CDS             11753..12382
                     /gene="nth"
                     /locus_tag="VXN30_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1357375.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12593..13114
                     /gene="pyrR"
                     /locus_tag="VXN30_000713"
     CDS             12593..13114
                     /gene="pyrR"
                     /locus_tag="VXN30_000713"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1357376.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13133..14056
                     /locus_tag="VXN30_000714"
     CDS             13133..14056
                     /locus_tag="VXN30_000714"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1357377.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14106..15185
                     /gene="carA"
                     /locus_tag="VXN30_000715"
     CDS             14106..15185
                     /gene="carA"
                     /locus_tag="VXN30_000715"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1357378.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15497..18673
                     /gene="carB"
                     /locus_tag="VXN30_000716"
     CDS             15497..18673
                     /gene="carB"
                     /locus_tag="VXN30_000716"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1357379.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5432 a         3605 c         4208 g         5441 t
ORIGIN      
        1 tgtgtgtaga ttcagggtca atttgaagag agtatttaaa atatcttggg aaattataat
       61 ttctccctgt tttttgagtc caatctatga gtatgagttg cccaggagta aggccacatt
      121 ctaattcgtt taggtaaaaa ttgttacaat cagaatttgt gtctctatta tgattatttt
      181 gttttaaatt aaatagtttg ctaaaaaaat tcattggatt ttattgctaa tcaattctta
      241 ttgaaaaatg atacttttac agtacccatg ttcggatggc atgggcaacg ccggattcgt
      301 catttgtttt ggtgatgtat ttggcgattt ttttgatttc tggatttcca ttttccatga
      361 caacggggtt tccaacgact tccagcatgg cacggtcatt ttcttcatca ccgattgcca
      421 tggtttcatc tttggtcaat ccgagttttt cagccaagtg agtaatggct gaacccttgt
      481 ctacattctt tttaaggagt tcgaggtaga aaggagcaga tttgttgatg gagtagcgct
      541 cgtaaaattc tgctggaatt ttttcaatcg cagcatcgag aatttctggt tcatcgataa
      601 acatacattt aacaatttct ttgccaacca tttcttcagg ggtacggtag aagataggca
      661 tgctgacgag ggttgattcg tgtacagtgt attttccgat attgcgattt gcagtataga
      721 taccgtcctt ggtaatggca tgcatgtgga caccgagctt gcgactgagg aattccatat
      781 ccagataatc ctcataggtc aaggattcgc tgataatctc atggccagta gcagtttctt
      841 ggacaagggc accattgaag gtcaccacat agtcaccttg gtctctcaac tgcaagtcgt
      901 ccagaagttt ggcaacacct gcgatggggc ggccagttgc aatcacgact ttgacaccag
      961 cttctttggc atcttggatg gcagaaaaga cttcaggagt gatttccttt tggctgttga
     1021 ctagggttcc gtcaatatcg acggcaatta gtttaatact cataaaattc ctcttctagt
     1081 ttttatttgg ggtaaaatga tcgttctcaa tcaagtgtaa aaattgctgg gtaatgctag
     1141 caaagatact gttttggtct aacatttctt tagggaaata gaaacgatta tctccgtggc
     1201 gactgccagc aagggattgg acgataagag acaggctaga gagttcggcc agttctccat
     1261 ttttttgtaa aatctcaatc tgtgtccgtg ggttttcaga ttcgggacga tagatatcat
     1321 aagggaggtc aaagttctta tgaatggcag tgtagtagtc gggatcaaag ccgatatcct
     1381 caaccaattt tctcatgcta gtaagttgat cttggtcctc ttgtgaaaag gtaatggatt
     1441 taaagacctt gcggttgaca aagcgatgcg ataaatctgc aagaatcttg tcaggactgg
     1501 tcatccaaag ctggaagtag gtattcatca cgccatcatc cagagccaga tagtcagtca
     1561 aggtcacatt tttttcgaag aaaggcagga ggtgtggaga agttcgggca aagaaatcct
     1621 tgtcctcagg atagagttcc ttggcgcgct tgagaagatt ctgtaggaga acttccatgg
     1681 cgcgtgttgc ggggtggaaa taaacctgca tgtacatctg gtagcgactg aggacgtagt
     1741 cttcgatggc gtgcatgcca ttgcgctgaa aggcgatacc attttcgata ggacgaatga
     1801 ctcggaggat gcgagtcagg tcaaattccc cataggatgc tcctgtaaaa taggagtcgc
     1861 gcaagagata gtccatgcga tctgcgtcaa tctgactaga aatgagctgc acgacctgct
     1921 tattaggata ggtatggtca atgacactgg ccaccttttc tgggaaatcg ggcgctactt
     1981 gtagcaagac ttggtgaatc tctgtctcag gattttgaat aatctcctga gtaatggctt
     2041 catggtctgt atcaaagaga tgttcaaaag tatgggagta ggcgccatgc ccaaggtcat
     2101 gtaggagagc agcggtcatg gtcaagagag actcggcagg attccattcc tcaggatatt
     2161 tttcttcaaa aatttctgtg atacgtcgtg caatctcata gactcccaga cagtgagaga
     2221 agcgactatg ctctccaccg tggaaggtat aactggaagt ccctagttgc ttgatccggc
     2281 gcaaacgttg aaattctttt gtattaatca agtcatagat gatttgatta ttgacatgga
     2341 tgtagttgtg aacagggtca cggaatactt tttcgttcat atgaccatta tagcaaaatc
     2401 ctgctctttt tggtaaaata gtaggacaag agacaagaaa gaggacctga tgtgaagatt
     2461 cggatgcgaa atacgattca gtttgatgag cagttggaag tgattgacca gctttatgat
     2521 gtggaagtgc atgaaaaggg agattatagc tatttactct tttataatga ggaaaaggaa
     2581 aaagtagtta ttaaatttca tggtcaagaa ctggtgatga gccgattttc taatcccaag
     2641 accattatgc gctttttaaa ggatagtgat agtttggcct atattcctac ccctatgggc
     2701 atgcaggaat ttatcattca aacgagccgt tatcaagttg ataggcaaaa gattgagcta
     2761 gcttatcaac tacaaaatca agaggggcat ccctttgcca gctatcaatt ggaaattact
     2821 tggggctagg atctcgtttt tctcaaatct actttctatc ttcaaggatt ggctccctag
     2881 tgtggtagat taggctattt tttggtataa taaaagttat ggaaatcgaa aaaaccaatc
     2941 gtatgaatgc gctctttgaa ttttatgcgg cgcttttgac agataagcaa atgaattata
     3001 tcgagctcta ctacgctgat gattacagcc ttgctgaaat tgccgaggag ttcggtgtca
     3061 gtcgtcaggc tgtctatgac aatatcaagc gaacagaaaa gattctggaa gattatgaga
     3121 tgaaattgca catgtactcg gactatattg tccgcagtca gatttttgat cagattttgg
     3181 agcgctatcc caaggataac tttctgcagg agcagataga aattttaaca agcattgata
     3241 atagagaata agaggaagaa aaatggcatt tgaaagttta acagaacgtt tgcagaacgt
     3301 ctttaaaaat ctacgtaaaa aaggaaaaat ctctgaatct gatgtccaag aggcaaccaa
     3361 agaaattcgc ttggccttgc tcgaggccga cgttgccttg cctgttgtaa aggactttat
     3421 caagaaagtt cgtgagcgtg cagtcgggca tgaggtcatt gatacactta atcctgcgca
     3481 acagattatt aaaatcgttg atgaggaact gacagccgtt ttaggttctg atacggcaga
     3541 aattatcaag tcacctaaga ttccaaccat catcatgatg gttggtttac aaggggctgg
     3601 taaaacaacc tttgctggta aattggccaa caaactcaag aaagaagaaa atgctcgtcc
     3661 tttgatgatt gcggcggata tttatcgtcc agctgccatt gaccagctta agaccttggg
     3721 acaacagatt gatgtgcctg tctttgcact tggaacagaa gtaccagctg ttgagattgt
     3781 acgtcaaggt ttggagcaag cccaaactaa tcataacgac tatgtcttga ttgatactgc
     3841 gggtcgtttg cagattgatg agctcctcat gaatgagctt cgtgatgtga aagcattggc
     3901 tcaaccaaat gaaatcttgc ttgtcgttga tgctatgatt ggtcaggaag cagccaatgt
     3961 tgcgcgtgag tttaatgctc agttggaagt gactggggtc atccttacca agattgatgg
     4021 cgatactcgt ggtggtgctg ctctgtctgt tcgtcacatt actggaaaac caatcaagtt
     4081 cactggtaca ggtgaaaaga ttacggacat tgaaaccttc cacccagacc gcatgtctag
     4141 ccgtatcctt ggtatggggg atatgctcac tttgattgag aaagcttctc aggaatacga
     4201 tgaacaaaaa gcccttgaaa tggctgagaa gatgcgcgaa aacacctttg attttaatga
     4261 tttcatcgat caattagatc aggtgcaaaa tatggggccg atggaagact tgctcaagat
     4321 gattccaggt atggccaaca atccagccct tcaaaacatg aaggtggatg aacgccagat
     4381 tgctcgtaaa cgtgccattg tgtcttcgat gacacctgaa gagcgtgaaa acccagattt
     4441 gttaaatcca agccgtcgcc gtcgtattgc tgctggttct ggaaatacat tcgtcgaagt
     4501 caataaattc atcaaggact ttaaccaggc taaacagctc atgcagggtg ttatgtctgg
     4561 ggatatgaat aaaatgatga agcaaatggg gattaatcca aataaccttc ctaaaaatat
     4621 gccaaatatg ggaggaatgg atatgtctgc ccttgaagga atgatgggac aaggcggtat
     4681 gcctgactta tcagctctcg gaggagcagg aatgccagat atgagccaga tgtttggtgg
     4741 cggtttgaaa ggtaaaattg gtgaatttgc catgaaacag tccatgaaac gtatggctaa
     4801 caaaatgaag aaagcgaaga agaaacgcaa gtaagacaag agattgatgc aaatctcatt
     4861 tgatgagaaa ataaagtaaa aatttctatg ataaaacgta cagtatcaag gttttagaat
     4921 atctgatatt atacgttttt tgattttaat aattttgtta agacactttt attatggtat
     4981 gcgaaaaatc cactcagctg tctgagtgga ttaggtgtat ttagactctt agtctttatt
     5041 ttcgtatggc aagatcaagt tcaagatgat tcctgtcatg gctgaaaggg cagtacctga
     5101 aagtgtaact ggaccaagtt taaggatagc tcctccaagt ccaagaacca acatagcact
     5161 tgcgatgatg aggtttcgca tttgagcgaa atcaacacgt tctttaatca agactttcaa
     5221 accattgctg gcgataaccc catagagaag gattgacata ccaccaagta cagcgtttgg
     5281 aatagttgaa atcaaggcag tgaatttacc aaggaagctg agggcaatcg cgatgaaggc
     5341 agcgttacgg ataactgaga cagaagcgat acgagtcata ccgataaccc ctgtattttc
     5401 tccgtaagtt gtattggctg gtccaccaag gaaggcagaa acagaagttg cgataccgtc
     5461 accaagaaga gtacggtgaa gacctggttc ttttaagaat tgacgaccac agatttgacc
     5521 caaaacagta tggtctccga tatgttcaga aattgttacg atagcgattg gcaagatagc
     5581 gatggcttct ggaccaaagt aaagattgta ctctttaaag gcaccacctg tgctaaatgg
     5641 caagtagaaa ccaggaattt cgaaccagtt ggctttaaga actggtgtaa agtcaaccaa
     5701 gccaagagtt agtgcgaaaa ggtaaccacc gataatggca aagaggaatg gaatgattcg
     5761 taggaagcct tttccttttg tattgataaa ggcagcaatt aggaaagtaa caacggctac
     5821 cagagcattt ttccaatttc cgtctgctac aagacctgca ttggtaacag ctgaacctgc
     5881 aagtccaaga ccgataacga tgatcatagg accgataatg attggtggca agagtttatc
     5941 aatccatttt gttcctacaa atcggatgct ggtagcaaca aggacataga ccaaaccagt
     6001 caagataacc cctgtttggg cagcagatac atcccccccc atttctttca tagccagtga
     6061 catagctgtg ataaaggcaa atgaagaacc tagataaact ggaactttaa aaccagtagc
     6121 aatcatgtag atgagtgttc caacacctga agcaaaaagg gcaacagata caggcattcc
     6181 caaaatcaat ggtaccaaga tggtcgcacc aaacatggcg aaaacgtgtt ggaaactaag
     6241 gagaattcct tttccatccg aaggacgttg gtcaacgtct agtaacaaat caacagttga
     6301 ttcttgtttc atataaacct cacttttggg catcagaaaa gagcccatta ttttacagca
     6361 ataggccctc agagtaagac ttctctaaaa tctagacttt aaagagtttc aaatatttct
     6421 gcgtcttcgt cacctcacgg gatgacatta aaaacctttg cttaggatag tatagcagaa
     6481 aaaaatgatt ttgtaaatca ttttttcccg agcctagaaa taaaagagcg aggctgattt
     6541 tgtaaatcat tttttcccga gcctagaaat aaaagagcga ggctgatttt gtaaacattt
     6601 gtttactaaa aaatttaaag tgggcgttta tttggcatac cagccttacg cggatattta
     6661 tttggtgttt cttttttctt ttctaccact gtgatatagc gcggatctct attcggtagg
     6721 gcgtagctga gattgtcttc gaccttacta aaaaggagat tgagggcatt cttagcttct
     6781 aataattcct caggcgcatt gctggccttg agtgccaata gttttccacc aaccttaagg
     6841 tagggaatgg tcaattcaga taggacctgc atacgggcaa ccgcacgagc tgttacaaaa
     6901 tcatattgag cacggaagtt cttgtcttgg gcaaaatctt cggcacgtcc gtggtagaaa
     6961 tgaactccgt tcaaatccag ttcttgagcc aagagttgta ggaagttgat gcgcttattg
     7021 agtgaatcaa taatggtcac atctaactcc ggatagagaa ttttcattgg tagactagga
     7081 aatcctgccc cagccccgat atcaagaagt ttgatagttt cattgggaat caaaccttga
     7141 agaatgggtg caatcgaatc gtaaaaatgt ttgagataaa cttcttcctt gtccgtaatc
     7201 gccgtcaaat taatcttctc attccactcg accaagagct caaaataacg ttcaaattgt
     7261 tctttttgct ggtccgaaag tggaagattc tgctcggcaa gcaagttgta aaatgttttt
     7321 ggtttcatag ttatttcctt ctttagtatc atcttatttt accacaatca tagaaaattt
     7381 gatatactgg tactaatcta aaagcagaaa ggacttatat tatgacttgg attattcttg
     7441 gagttatcgc tcttattgtt atttttgtga ttgttagcta taacggtttg gttaaaaatc
     7501 gtatgcaaac caaggaggct tggagtcaga ttgatgttca gttgaaacgt cgaaatgacc
     7561 tcttgccaaa cttgattgag actgtaaaag gttatgccaa atatgaaggt tctacccttg
     7621 aaaaggtggc agaactacgt aaccaagtgg cggcagcgac ttcaccagca gaagctatga
     7681 aagccagtga tgccctcact cgtcaggttt caggtatttt tgcagttgca gaaagctatc
     7741 cagatttgaa agctagtgct aactttgtta aattgcaaga ggagttgaca aacacagaaa
     7801 ataaaatttc ttactctcgt caactctata acagtgttgt cagcaactac aatgtaaaat
     7861 tagaaacttt cccgagcaat attatcgctg gaatgtttgg atttaaagcg gcagatttcc
     7921 ttcaaacacc tgaagaggaa aagtcggttc ctaaagttga ttttagcggt ttaggtgact
     7981 aagatgttgt ttgatcaaat tgcaagcaat aaacgaaaaa cctggatttt gttgctggta
     8041 tttttcctac tcttagctct tgttggttat gcggttggtt atctctttat aagatctgga
     8101 cttggtggtt tggttattgc actgattatc ggctttatct acgctttgtc tatgattttt
     8161 caatcgacag agattgtcat gtccatgaat ggagcgcgtg aggtggatga gcaaacggca
     8221 ccagacctct accatgtagt ggaagatatg gctctggtcg ctcagattcc tatgccccgt
     8281 gttttcatca ttgatgatcc agccttaaat gcctttgcga caggttctaa tcctcaaaat
     8341 gcggctgttg ctgcgacttc aggtctacta gctatcatga atcgtgaaga actagaagct
     8401 gttatgggac atgaagtcag tcatattcgt aattatgata tccgtatttc gactattgca
     8461 gttgcccttg ctagtgctat caccatgctt tctagtatgg caggtcgtat gatgtggtgg
     8521 ggtggagcag gtcgcagacg aagtgatgat gaccgagatg gaaatggtct tgaaatcatt
     8581 atgctagtgg tttccctact agctattgta ctggcacctc tcgctgcaac cttggttcag
     8641 ctcgctattt ctcgtcagag ggaatttctg gcagatgcat ctagtgtcga gctgactcgc
     8701 aatccccagg gaatgattaa tgccctagat aagttggaca atagcaaacc tatgagtcgc
     8761 cacgtcgatg atgctagcag tgccctttat atcaatgatc ctaagaaagg tggggggttc
     8821 caaaaactct tttataccca cccacctatc tcagaacgga ttgaacgttt aaaacagatg
     8881 taaaagaatc cagaggtcgt ctctggattt ttgctatgtt gcaaatttat aaatcttcta
     8941 aattaactag cttcaaaccg tgttctgtca tgcgatagat gatcgagcag acttctttta
     9001 agaaacgacg gtcatgcgaa acagtgatga gaccgcctgg ataggttgca aagagttttc
     9061 tgatttgggg ttgagaagtg ggagaaaagt ttcgtgtggg ttcatccagc aggagaaagt
     9121 ttggtttgcg caggactaga tccaaaagca ggagttttcc ctgttgtccg ccagataagg
     9181 agcgaatttg atgctgcatt tctggataac tgaaattgag actggctagg tgagattgga
     9241 ttttctgaag ttcctctttt tccccagttt tgctgatata agctattggt gataaatcca
     9301 attgcagttt tttgtggtaa tcttgtggca taaaaccaag ggaaatctct cttttatcat
     9361 tcagaagttg ctttaatttg gctaacagag ttgatttccc aacaccattt ggcccgataa
     9421 taccgatttt ttcttgccca cggacagtta gttgtagttt ttgaaccaaa actcggtcgt
     9481 caatggacaa attttctttt tccagttgga ctaagacttt agaagctggt aatggttgga
     9541 tgtctgaaaa gaaaagttgg atttgttcct cttcaagtgg cttttgagtc atggactgag
     9601 ctgccttttc gtagcgtttt tcttgtgaga ggacagtttt catcttttta gccaataggc
     9661 gaccggcagt actatctttg gtagctcgaa gcgcagtttc tacattttgc ttaactctcc
     9721 gatgtttttc catggtttta tcgtaggctc tttggttgtt agcagcttgc tgactttgtt
     9781 tggcaaaatt agcctttctc tgctcactat agctatcata gtctaaatgc tctactagcg
     9841 tttccgcttc tttacggtgt ttgaccagtc gcaagtgaac aatagtgtct gccgtttcag
     9901 aaagaaagtc ttcatcatgg gaaatgaaaa taacggtttg tctggtcttt tgaatctggc
     9961 cttttagcca atcaactgtc tcaaggtcta ggtcatttga aggttcatct aaaaatagaa
    10021 tctcaaaggg tttggctaac tcatggataa gctgaatttt caaagcttct ccccctgata
    10081 gattgctaat ctcttggtca cttgcgaaac gattgctatc aaaatgcaat tcctccgcca
    10141 aacgatagag gatactgtag tctaaatcaa tagaatctaa aaagaagtag tcgtgtaaag
    10201 ttttcttttt cagctcctcg ggaagttttt gaggaatgta ggccagtgac tgatagtcag
    10261 attggatgtt tcccttgata gtgaaatcag acaaagcttc ccccattaaa attttaagta
    10321 aggttgattt gccatttcct tcttcaccaa taatagcaac cttttccccg tcttggatgg
    10381 tcatggttag gtcagataca agatctcgta aatctttgtt ttgtgtgatg gttagatgat
    10441 tgatttttat catatgattt ccctttctag aatgttcata gtgcataaca aaaagctcta
    10501 ctttacctag tagagcccaa agtctatgtc aaaaaaactc tattaacatg ccagaaaaag
    10561 agcataaaag agcacacaaa aagagacaaa aacaagatct actaaataaa ggttctttat
    10621 ttgatagact tagttgttca tgtctccctt acctccgagt attagtatct ttatattata
    10681 cctttaagga aattttgtca acagaaaact ggaaattcta tgctctaaaa ttcaaaaaca
    10741 ggtccataga gacttagtac gtgtttgaca tgctcgtaat ggaatttatc atagtttcgc
    10801 caagcagtgc gtgcttgatt gtttagtttt aggctaccca gtccaatcag aggactggtg
    10861 ggttggtaaa atttctcaag atcaattaag atactgttgt tcaacttttc cgtttttttg
    10921 agctcttgca tagtagtgtt tagcagctct tgtagtcgaa tatcatctgc cgtaccctgt
    10981 ttacagtttt ggtagctttt atttaacttg gataggagtt gaatgagttt cttgtcttcc
    11041 atgtgagcat cctccttgat ctgatgtact ctttccctat agtatagcac taaaaaacaa
    11101 aactgtcata gtaaatatgt taaaatttga attttaatta caaagtttgg tgtgctgatt
    11161 tgtagccttt tcatggtata atcaagagag taaatcttta tggaggaagt atgaaattaa
    11221 atattcaaga aattcgtaag cagtctgaag gtttgaactt tgaacaaacg ttagatttag
    11281 ttggtgacct gcgtgcacgt aatcaagaaa ttttagatgt aaaagatatc cttgcagttg
    11341 ggaaagtaca atatgaagac cgtatgtatt tcttagatta tcaactatct tataccattg
    11401 ttcttgcttc gagtcgcagt atggagccag ttgagttagt tgaatcttat ccagtcacgg
    11461 aagttttcat ggaaggcgca actaaccagc tagatcaaga agttttagat gatgacttgg
    11521 tcttgcccat cgaaaatggg gagcttgacc ttgctgagag tgtatcagac aatatcctgc
    11581 taaacattcc tatcaaggtc ttgacggctg aagaagaagc tggtcaagga tttatctcag
    11641 gaaatgactg gcaaatcatg acagaggaag aataccaagc tcaaaaagca gtaaagaaag
    11701 aagaaaacag tccttttgct ggcttacaag gactatttga cggagatgaa taatggtctt
    11761 gtcaaaaaaa cgagcacgaa aggtgctaga agaaatcatt gccctcttcc cagatgccaa
    11821 gcctagtctt gattttacca atcattttga actcctggtt gcggtcatgt tatcagccca
    11881 gacgacagat gcagcggtaa ataaggccac accaggtctc tttgttgcct ttccaacacc
    11941 ccaagctatg tctgtagcga cagagagtga gattgcttca catatttctc gcttgggatt
    12001 gtatcggaat aaagctaaat tccttaaaaa atgtgcccaa cagttactag acgattttga
    12061 tggtcaagtc cctcagacac gtgaagaatt ggagagtttg gcaggtgttg gtcgcaagac
    12121 agccaatgtt gtcatgagtg taggatttgg gattccagcc tttgcagtgg atactcatgt
    12181 ggagcgtatt tgcaaacacc acgatattgt caaaaaatca gcgacgccac ttgaggtgga
    12241 aaagcgggtc atggatatct tgccgcctga gcagtggtta gctgcccatc aggccatgat
    12301 ttattttgga agagccattt gccatccaaa aaatccagag tgtgaccagt acccacaatt
    12361 atatgatttt agcaatttgt aagatgctac attgaaactt tttgcttgat tttaagcatg
    12421 ctttgtgcta tagtaaatga taactaaata ttcctttaaa cctgtcccgt gaggcaggca
    12481 aggagcgcga taaagtagaa gggtgaagtc tatactgtgt gcagtagggc tcgcttggct
    12541 ataaatttca agtctccttg tttaaggaga cttttctttt tggaggtttg tcatgaaaac
    12601 aaaagaagtt gtagacgaat tgaccgtcaa acgagcgatt acgcgtatta cttatgagat
    12661 tatcgaacgc aacaaagatt tgaataagat cgtcttggct ggtattaaaa ctcgtggtgt
    12721 ctttattgcc caccgaatcc aagaacgttt gaagcagtta gaaaatcttt cagttcctgt
    12781 tgtggaattg gatactaaac ctttccgtga tgatgttaaa agtggagaag atacttcttt
    12841 ggtttctgtc gatgtgacag accgcgaagt tatcttggtg gatgatgtgc tctatacagg
    12901 tcgtaccatc cgtgctgcta ttgataatat tgtcggtcat ggtcgtcctg cgcgtgtgag
    12961 tttagcagtt ctagtcgatc gtggacatag agaattgcca atccgtccag attacgttgg
    13021 aaaaaatatc ccaaccagtc gttctgaaga aatcatcgta gagatgacag aacttgatga
    13081 ccaagacaga gttctgatta ctgaagaagc ttagaaagtt aaaggagtag ccatgtcaga
    13141 aaatcaacaa gcattgaacc atgtggtgtc catggaagac ctcactgtcg atcaagtaat
    13201 gaaattgatc aagcgaggaa ttgagtttaa aaatggagcc cagcttccct atgaagacca
    13261 tccgattgtt tccaatctct tctttgagga ttctacacgg acacataagt cctttgaagt
    13321 cgcagagatt aaacttggat tggaacgact tgactttgat gtgaagacta gttcggttaa
    13381 taagggtgag acactttatg acaccatttt gactctgtct gctttaggag tggatgtctg
    13441 tgtgattcgc catccagagg tcgactacta tagagaattg attgctagtc caacgattac
    13501 gacttccatc atcaatggtg gagatggttc gggccaacac cctagccaga gcttgcttga
    13561 tttgatgacc atttatgagg aatttggcca ctttgagggt cttaaagttg ctattgcagg
    13621 tgacttggac cactcacgcg ttgccaaatc caatatgcag attttgaaac gcttgggagc
    13681 tgaactcttt ttcgctggac ctgaggaatg gagaagtcaa gagtttgcag actatggaaa
    13741 gtttgtaacc attgatgaaa tcattgatca ggttgatgtc atgatgtttc tccgtgtgca
    13801 acacgaacgc catgatagtg gagctgtatt ttcaaaagaa gactaccatg cccaacatgg
    13861 cttgactcaa gaacgttacg atcgtttgaa agaaacagca atcctcatgc acccagctcc
    13921 aatcaatcgt gatgtagaaa tagcagacca cttggttgaa gcaccaaaat cacggattgt
    13981 ccaacaaatg accaatggtg tctttgttcg aatggcaatc ttagaatccg tactagcgag
    14041 tagaaacgcc aactaaaaca caagacgtta aaagacgcca gtgagcgtcc acgagaggtg
    14101 aaaatatgac aaaaagaatt ctagtattag aagatggcac agtttttgaa ggcaaggcct
    14161 tcggagcaga tattgatgta acaggcgaaa tcgtctttaa tacagggatg accggctacc
    14221 aagaatccat tacagaccag tcttataatg gacaaatctt gacctttact tatcctttgg
    14281 taggaaatta tggtattaat cgtgatgatt atgaatccat tattccaact tgtaagggag
    14341 tcgttgtttt tgaagaagcg cgtagagcta gcaactggcg caaccaaatg accttggatg
    14401 aatttttgaa agccaagaaa attccaggga tttcaggaat tgatacgcgt gctcttacca
    14461 agattatccg taagcatggt actatgcgtg caaccttgac ccatgttggg gacagtatgg
    14521 accatgtgac ggaccagctc caagcaacag ttttaccgac agacaatatc aaacaggttt
    14581 ctactaaaac gtcttatcca gctccaggag ttggtttgag cgtggtgcta gtggactttg
    14641 gtctcaagca ctcaatccta cgtgaacttt ctaaacgcaa ctgtaacgtg acagttgttc
    14701 cttattcgac aacggctgaa gaaattctcc atctcaatcc tgatggagtt atgttgtcaa
    14761 atggtccagg taacccagaa gacgttccac aagcactcga catgattcgc ggtgtacaag
    14821 gaaaaattcc aatctttggt atttgtatgg gacaccaact ctttgcaatg gcaaacgggg
    14881 ctaagaccta taagatgaag tttggccacc gtggattcaa ccatgcggta cgtgaaatcg
    14941 caacaggacg agtagacttc accagccaga accatggtta tgcagtcagc cgcgaggatt
    15001 tgccagagca cttgattatt acccacgaag aaatcaatga caagtcagtg gaaggtgtgc
    15061 gccacagata ccaacctgct ttctctgttc aataccaccc agatgcagct cctgggccac
    15121 acgacgctag ctacctattt gacgagttta tcgagatgat ggaagttttt aaacaatcaa
    15181 actaagaacg agtaaaagct cgtcggagaa tttatgtaac tgaacacgga cggaaagctc
    15241 agaatgtaga gaaaccaact tggattgtca atccttcctt gattttctaa aattcaatcg
    15301 ctttctattc gtcctttgta tcttatttaa tgtcgccctg agaggcgacg aatagaaagg
    15361 agaagataca ttgttcgcgg aagtgaacac gccctaagaa ctgtgtgaaa aagataaaca
    15421 tcgtcttgac gctgaaggcg tctgcgccga gtttcctatt ttcactgtgt tctttacggg
    15481 ctttgtatca taaactatgc ctaaacgtac tgatattcaa aaaattatgg tgattggttc
    15541 tggtccgatt attattggtc aggctgctga gtttgactac gctgggaccc aggcttgctt
    15601 gtcgttgaaa gaggaaggtt atgaggttgt tttggttaac tcaaatcctg caaccatcat
    15661 gacggacaag gaaattgctg acaaggttta cattgaaccg attacacttg agtttgtgac
    15721 acgtattctt cgtaaggaac gtccagatgc cttgctacca acactcggtg gtcagacagg
    15781 tctcaacatg gccatggaat tatctaaaaa tggtatccta gacgagcttg gcgttgaact
    15841 tctgggtacc aaattatctg ccattgacca agcggaggac cgtgacctct ttaaacaatt
    15901 gatggaagag cttgagcaac ccattccaga atctgaaatt gttaacacag tagaagaagc
    15961 agttgccttt gcagcgacaa ttggctaccc agtcatcgtt cgtccagcct ttacccttgg
    16021 tggtactggt ggtggtatgt gtgccaacga aaaagaattg cgtgaaatca ctgaaaatgg
    16081 tttgaaattg tcacctgtta cccaatgttt gattgagcgt tcgattgccg gttttaagga
    16141 aattgaatac gaagtgatgc gtgactcagc tgacaatgcc ttggttgttt gtaacatgga
    16201 aaactttgac ccagttggga ttcacacagg ggattccatt gtatttgccc ctgcgcaaac
    16261 catgtcagac tatgaaaacc aaatgcttcg tgatgcgagc ttgagcatta ttcgcgccct
    16321 caagattgaa ggtggatgta atgttcagct agctcttgat cctaacagct tcaagtatta
    16381 tgttatcgaa gtaaaccctc gtgtatcacg ttcgtcagcc cttgcttcta aagcgacagg
    16441 atatccgatt gctaagttgg ctgccaagat tgcagtaggt ttgaccttgg atgaggttat
    16501 aaacccagtt acaggttcaa cctatgccat gtttgaacca gcccttgact acgtagttgc
    16561 taagattcca cgtttcccat ttgacaagtt tgaaaagggt gaacgccgtc tcggtacaca
    16621 aatgaaggcg actggagaag tcatggcaat cggtcgtaac atcgaggaat ctcttctcaa
    16681 ggcctgccgc tcccttgaaa ttggggtgca ccacaatgaa atacctgaac ttgcagcagt
    16741 ttctgatgat gccttgattg aaaaggttgt caaagcccaa gatgaccgtc tcttctacgt
    16801 atcagaagcc attcgtcgtg gctacacacc tgaagaaatt gcagagctta caaaaattga
    16861 tatcttctat cttgataaac tcttgcatat ctttgaaatt gagcaagaat tgggtgctca
    16921 tccacaagat ctagaagttt tgaaaacagc caaacttaat ggtttctcag accgtaagat
    16981 tgctgaactc tggggaacga ctgatgatca agttcgccaa cttcgcttgg aaaacaagat
    17041 tgttccagtt tataagatgg tagatacttg tgcggcagag ttcgactctg aaacgccata
    17101 tttctattca acctatggtt gggaaaatga atctatcaga tctgataaag aatctgtact
    17161 tgtcctaggt tcaggtccaa tccgtatcgg tcaaggggtt gagtttgact acgcaaccgt
    17221 tcactctgtt aaggctatcc aggcagctgg ttatgaagct atcatcatga actcaaaccc
    17281 agagaccgtt tcaacagact tctctgtatc agataagctt tactttgagc cattgacatt
    17341 cgaagatgtt atgaacgtca ttgacttgga acaaccaaag ggagttatcg ttcagtttgg
    17401 tggtcaaaca gccatcaacc ttgcggagcc attggcaaaa gcaggtgtga ccatccttgg
    17461 tacacaggtt gctgacctag accgtgccga agaccgtgac ctcttcgagc aagctctcaa
    17521 agaattggat attccacagc caccaggaca aacggctacc aatgaagaag aagcagctct
    17581 tgcagctcgt aagattggct tcccagttct tgtccgccca tcttatgtac ttggtggacg
    17641 tgccatggaa atcgttgaaa acgaagaaga ccttcgttct tacatgcgaa ctgctgttaa
    17701 ggctagtcca gatcacccag ttcttgtcga ctcttatatc gttgggcaag agtgcgaagt
    17761 tgatgccatt tcagatggta aagatgtgct cattcctggt atcatggagc atatcgaacg
    17821 tgctggtgtc cactcaggtg actcaatggc tgtttaccca ccacaaacct tgtcgcaaaa
    17881 ggttcaggag accatcgcag actacaccaa acgcttagca atcggtcttc actgccttgg
    17941 aatgatgaac atccagtttg ttatcaagga tgaaaaagtc tacgttattg aggtcaatcc
    18001 acgtgccagc cgtactgttc cattcctatc taaggtaacc aatattccta tggctcaagt
    18061 agcgaccaag ctcattcttg gtcaaagttt atcagaactt ggctaccaaa atggacttta
    18121 ccctgaaagc acccgtgttc atatcaaggc acctgtcttc tcctttacca aactagctaa
    18181 ggtagacagc ttgctcggtc ctgaaatgaa gtcaacaggt gaagttatgg gttctgatgc
    18241 tactttggaa aaagctctct ataaagcctt tgaagcttct tatctacact tgccaacttt
    18301 tggtaatgtc gtctttacca tcgctgatga tgccaaagaa gaagccttga acttggctcg
    18361 tcgtttccaa aacattggtt atggaatcct tgcgacagaa gggacagcag ccttctttgc
    18421 tagtcatggc ttgcaagccc aacctgttgg taagattggt gatgacgata aggatatccc
    18481 aagttttgta cgcaaaggaa gaattcaagc tatcattaac acagtaggaa caaaacgaac
    18541 tgctgacgaa gatggtgagc aaattcgtcg ttcagccatt gaacacgggg tgcccctctt
    18601 cacagcgcta gatacagcta atgccatgct taaggtactt gaaagtcgta gctttgttac
    18661 agaagcgatt taaatagaaa aataag
//
LOCUS       DAVLSL010000026        18633 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLSL010000026 DAVLSL010000000
VERSION     DAVLSL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.026"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            68..5371
                     /locus_tag="VXN30_000717"
     CDS             68..5371
                     /locus_tag="VXN30_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1357380.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPTTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTASTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVILFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDTDNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPIVEKPEFKLNSLASEQGKTPDYKQEIA
                     RPETPAQILPATGGSQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(5538..7697)
                     /locus_tag="VXN30_000718"
     CDS             complement(5538..7697)
                     /locus_tag="VXN30_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1357381.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAINTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTEMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIEKHIKNTGYVAPDETFVGYTRKYSMAVWTGYTNRLTPIMGDGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7694..8290)
                     /gene="recU"
                     /locus_tag="VXN30_000719"
     CDS             complement(7694..8290)
                     /gene="recU"
                     /locus_tag="VXN30_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1357382.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8356..8883
                     /locus_tag="VXN30_000720"
     CDS             8356..8883
                     /locus_tag="VXN30_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1357383.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            8953..9282
                     /gene="gpsB"
                     /locus_tag="VXN30_000721"
     CDS             8953..9282
                     /gene="gpsB"
                     /locus_tag="VXN30_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1357384.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9303..9683
                     /gene="rnpB"
                     /locus_tag="VXN30_000722"
     ncRNA           9303..9683
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VXN30_000722"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9768..10925
                     /locus_tag="VXN30_000723"
     CDS             9768..10925
                     /locus_tag="VXN30_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1357385.1"
                     /translation="MKKKFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEATKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10938..12332
                     /gene="mapZ"
                     /locus_tag="VXN30_000724"
     CDS             10938..12332
                     /gene="mapZ"
                     /locus_tag="VXN30_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1357386.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12408..13832
                     /gene="gndA"
                     /locus_tag="VXN30_000725"
     CDS             12408..13832
                     /gene="gndA"
                     /locus_tag="VXN30_000725"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1357387.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13844..14533
                     /locus_tag="VXN30_000726"
     CDS             13844..14533
                     /locus_tag="VXN30_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1357388.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            14631..15653
                     /gene="cbpC"
                     /locus_tag="VXN30_000727"
     CDS             14631..15653
                     /gene="cbpC"
                     /locus_tag="VXN30_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1357389.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDPSNGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            15672..16670
                     /gene="cbpJ"
                     /locus_tag="VXN30_000728"
     CDS             15672..16670
                     /gene="cbpJ"
                     /locus_tag="VXN30_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV1357390.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGMRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDSTTGIMQTGWQYL
                     GNNWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYYVNYNGEWSR"
     gene            complement(16788..18020)
                     /locus_tag="VXN30_000729"
     CDS             complement(16788..18020)
                     /locus_tag="VXN30_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1357391.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLCLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFMHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGISVQLALIFLYL
                     VLIALIFCQWLVKFNTHN"
     gene            complement(18063..18633)
                     /locus_tag="VXN30_000730"
                     /pseudo
     CDS             complement(18063..18633)
                     /locus_tag="VXN30_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
BASE COUNT         5765 a         3371 c         4266 g         5231 t
ORIGIN      
        1 gaggaaggag tattttttaa aaaggaaatg taaacgcttg ctaaaaaatg aaaggattta
       61 gaagtttatg aataaaggat tatttgaaaa acgttgtaaa tatagtattc ggaaattttc
      121 attaggtgtt gcttctgtta tgattggagc tgcattcttt gggacaagtc cggttcttgc
      181 agatagcgtg cagtctggtt ccacggcgaa cttaccagct gatttagcta ctgctcttgc
      241 aacagcaaaa gagaatgatg gacgtgattt tgaagcgcct aaggtgggag aagaccaagg
      301 ttctccagaa gttacagatg gacctacgac agaagaagaa ctattagcac ttgaaaaaga
      361 aaaaccggct gaagaaaaac caaaagagga taaacctgca gctgctaaac ctgaaacacc
      421 taagacggta acccctgaat ggcaaacggt agagaaaaaa gaacaacagg gaacagtcac
      481 tatccgagaa gaaaaaggtg tccgctacaa ccaactatcc tcaactgctc aaaatgataa
      541 cgcaggcaaa ccagctctgt ttgaaaagaa gggcttgacc gttgatgcca atggaaatgc
      601 aactgttgat ttaaccttca aagatgattc tgaaaagggc aaatcacgct ttggtgtctt
      661 cttgaaattt aaagatacca ataataatgt ttttgtcggt tatgacaagg atggctggtt
      721 ctgggagtat aaatctccaa cagctagcac ttggtataga ggtagtcgtg ttgctgctcc
      781 tgaaacagga tcaacaaacc gtctctctat cactctcaag tcagacggtc agctaaatgc
      841 cagcaataac gatgtcattc tctttgacac agtgactcta ccagctgcgg tcaatgacca
      901 tcttaaaaat gagaagaaga tccttctcaa ggcgggctct tatggcaatg accgtacggt
      961 tgtcagcgtt aaaacagaca atcaagaagg cgtaaaagcg gatgatactc ctgcccagaa
     1021 agaaacaggt ccggttgttg acgatagcaa ggtgacttat gatacgatcc agtctaaggt
     1081 acttaaggca gtaattgacc aagccttccc tcgtgtcaag gaatacacct tgaatggaca
     1141 tactttgcca ggacaggtgc aacagttcaa ccaagtcttt atcaataacc accgaatcac
     1201 ccctgaagtc acttataaga aaatcaatga gacaacagca gagtacttga tgaagattcg
     1261 cgatgatgct cacttaatca atgcggaaat gacagtacgc ttgcaagttg tggacaatca
     1321 attgcacttt gatgtgacca agattgtcaa ccacaatcaa gtcactccag gtcaaaagat
     1381 tgatgacgaa agaaaactac tttcttctat tagtttcctc ggcaatgctt tagtctctgt
     1441 ttctagtgat caaactggtg ctaagtttga tggggcaacc atgtcaaaca atacgcatgt
     1501 cagcggagat gatcatatcg atgtaaccaa tccaatgaag gatttggcta agggttacat
     1561 gtatggattt gtttctacag ataagcttgc tgctggtgtt tggagtaact ctcaaaacag
     1621 ctatggtggt ggttcgaatg actggactcg tttgacagct tataaagaaa cagtcggaaa
     1681 tgccaactat gtaggaatcc acagctctga atggcaatgg gaaaaagctt ataagggcat
     1741 tgttttccca gaatacacga aggaacttcc aagtgctaag gttgttatca ctgaagatgc
     1801 caatgcagac aagaacgttg attggcaaga tggtgccatt gcttatcgta gcattatgaa
     1861 caatcctcaa ggttgggaaa aagttaagga tatcacagct taccgtatcg cgatgaactt
     1921 tggttctcaa gcacaaaatc cattccttat gaccttggat ggtatcaaga aaatcaatct
     1981 ccatacagat ggtcttgggc aaggtgttct ccttaaagga tatggtagcg aaggccatga
     2041 ctctggtcac ttgaactatg ctgatattgg taagcgtatc ggtggtgtcg aagacttcaa
     2101 gaccctaatt gagaaggcta agaaatatgg agctcatcta ggtatccacg ttaacgcttc
     2161 agaaacttat cctgagtcta aatacttcaa tgaaaaaatt ctccgtaaga atccagatgg
     2221 aagttatagc tatggttgga actggctaga tcaaggtatc aacattgatg ctgcctatga
     2281 cctagctcat ggtcgtttgg cacgttggga agatttgaag aaaaaacttg gtgacggtct
     2341 cgactttatc tatgtggacg tttggggtaa tggtcaatca ggtgataacg gtgcctgggc
     2401 tacccacgtt cttgctaaag aaattaacaa acaaggctgg cgctttgcga tcgagtgggg
     2461 ccatggtggt gagtacgact ctaccttcca tcactgggca gctgacttga cctacggtgg
     2521 ctacaccaat aaaggtatca acagtgccat cacccgcttt atccgtaacc accaaaaaga
     2581 tgcttgggtt ggggactaca gaagttatgg tggtgcagcc aactatccac tgctaggtgg
     2641 ctacagcatg aaagactttg aaggctggca aggaagaagt gactacaatg gctacgtgac
     2701 taacctcttt gctcatgacg tcatgactaa gtacttccaa cacttcactg taagtaaatg
     2761 ggaaaatggt acaccggtga ctatgaccga taacggtagc acctataaat ggactccaga
     2821 aatgcgagtg gaattggtag atactgacaa taataaagta gttgtaactc gtaagtcaaa
     2881 tgatgtcaat agcccacaat atcgcgaacg tacagtaacg ctcaacggac gtgtgatcca
     2941 agatggttca gcttacttga ctccttggaa ctgggatgca aatggtaaga aactttctac
     3001 tgataaggaa aagatgtact acttcaatac gcaggccggt gcaacaactt ggacccttcc
     3061 aagcgattgg gcaaagagca aggtttacct ttacaagcta actgaccaag gtaagacaga
     3121 agagcaagaa ctaactgtaa aagatggtaa aattacccta gatcttctag caaatcaacc
     3181 atacgttctc tatcgttcaa aacaaaccaa tcctgaaatg tcatggagtg aaggcatgca
     3241 catctatgac caaggattta acagtggtac cttgaaacat tggaccattt caggcgatgc
     3301 ttctaaggca gaaattgtca agtctcaagg ggcaaacgat atgcttcgta ttcaaggaaa
     3361 caaagaaaaa gttagtctca ctcagaaatt aactggcttg aaaccaaata ccaagtatgc
     3421 cgtttatgtc ggtgtcgata accgtagtaa tgccaaggca agtatcactg tgaatactgg
     3481 tgaaaaagaa gtgactactt ataccaataa gtctctcgcc ctcaactatg taaaagccta
     3541 cgcccacaat acacgtcgtg acaatgctac agttgacgat acaagttact tccaaaacat
     3601 gtacgccttc tttacaactg gatcggacgt ctcaaatgtt actctgacat tgagtcgtga
     3661 agctggtgat caagcaactt actttgatga aattcgtacc tttgaaaaca attcaagcat
     3721 gtacggagac aagcatgata caggtaaagg caccttcaag caagactttg aaaatgttgc
     3781 tcagggtatc ttcccatttg tagtgggtgg tgttgaaggt gttgaagata accgcactca
     3841 cttgtctgaa aaacacgatc catatacaca acgtggttgg aatggtaaga aagtcgatga
     3901 tgttatcgaa ggaaattggt cactcaagac aaatggacta gtgagccgtc gtaacttggt
     3961 ttaccaaact attccgcaaa acttccgttt tgaagcaggt aagacctacc gtgtaacctt
     4021 tgaatacgaa gcaggttcag acaataccta tgcttttgta gtcggtaagg gagaattcca
     4081 gtcaggtcgt cgtggcactc aagcaagcaa cttggaaatg catgaattgc caaatacttg
     4141 gacagattct aagaaagcca agaaggcaac attccttgtg acaggtgcag aaacagggga
     4201 tacttgggta ggtatctact caactggaaa tgcaagtaat actcgtggtg attctggtgg
     4261 aaatgccaac ttccgtggtt ataacgactt catgatggat aatcttcaaa tcgaagaaat
     4321 taccctaaca ggtaagatgt tgacagaaaa tgctctgaag aactacttgc caacggttgc
     4381 catgactaac tacaccaaag agtctatgga tgctttgaaa gaggcggtct ttaacctcag
     4441 tcaggccgat gatgatatca gtgtggaaga agcgcgtgca gagattgcca agattgaagc
     4501 cttgaagaat gctttggttc agaagaaaac ggctttggta gcagaagact ttgcaagtct
     4561 tacagctcct gctcaggctc aagaaggtct tgcaaatgcc tttgatggaa acttatctag
     4621 tttatggcat acatcatggg gcggaggaga tgtaggcaag cctgcaacca tggtcttgaa
     4681 agaaccaact gaaatcactg gacttcgtta tgttccacgt ggatcaggtt caaatggtaa
     4741 cttgcgtgat gtgaaacttg ttgtgacaga tgagtctggc aaggagcata cctttactgc
     4801 aactgattgg ccagataaca ataagccaaa agacattgat tttggtaaga caattaaggc
     4861 taagaaaatt gtccttacag gtactaagac ttacggagat ggtggcgata aataccaatc
     4921 tgcagcggaa ctcatcttta ctcgtccaca ggtagcagaa acacctcttg acttgtcagg
     4981 ctatgaagcg gctttggcta aggctcagaa attaacagac aaagacaatc aagaggaagt
     5041 agctagcgtt caggcaagca tgaaatatgc gacggataac catctcttga cggaaagaat
     5101 ggtggaatac tttgcagatt atctcaacca attaaaagat tctgctacga aaccagatgc
     5161 tccaattgta gagaaacctg agtttaaact taactcttta gcttccgagc aaggtaagac
     5221 gccagattat aagcaagaaa tagctagacc agaaacacct gcacaaatct tgccagcaac
     5281 aggtgggagt caatctgaca cagccctctt cctagcaggt gttagcctag ccctatctgc
     5341 tctctttgta gtaaaaacga agaaagacta gtatttagta aaacctctta acaagattac
     5401 ggaagcagtc tctatctttt ccaatgaggt ttatagtaca gaaaaagcct gagaagatgt
     5461 cttctcaggc ttttgttaag cacataaata caatagtgct atgacaaaat cacccagaaa
     5521 aatctgggtg ataaatgtta tggttgtgct ggttgaggat tctgattttg ttgatcaggg
     5581 gttgtatttg attgttgcgt attattgtta ggattggtag tcgtactatt atttgtgctt
     5641 ggagtggttg agctagactg tgaagttgaa ctatctgatg atgagcttga actttcagtt
     5701 gatgggggtt gttgtggagc aggtgagttc cacgtagaac gagcaccatt tttaaatacg
     5761 aattctccat ttctgtagag cccctctggt atattccaat cctctggatt gcttccttca
     5821 gacagatagg tcatcataga gcggtaaact ttggcagcaa ctgtgagacc atcccccata
     5881 attggtgtga gacggtttgt gtaacctgtc catacagcca tagaatactt acgagtataa
     5941 ccaacaaacg tttcatctgg agctacatag ccagtgtttt tgatgtgttt ttcaatttct
     6001 tcatctgtat agttagaggt acctgtttta ccagcttgag gaagccaagc aagataggca
     6061 tttcgtccag ttccataact caagactgtt ttcatcattt cggtcatcat ataggctgtc
     6121 gtttccttca tggcacgagt tccgacatta gagaactctt tttcactccc atcactaaag
     6181 acgactttat ggatatacat tggtttatag taagttccac catttgcaaa ggcagcgtaa
     6241 gcagcagcca tcttttcact acttgctcca tattttttgt ctgattcggt tgtgttactt
     6301 gaaatggcat ttgagtagtg aatacttggg tagtcgattc cgagaccatt taggaaagtc
     6361 ttggcgcggt tgagtccgac cttgtttaga gtttccacgg ctgggacgtt tcgcgattgt
     6421 tgcagggcgt attgcaaggt gatgttgcca aagtagcccc tatcccagtt ataaacagga
     6481 gtatttgtcc cagggtagtt atagggctca tcgtgaacga tagtagcagt tgaatcgtag
     6541 acaccgtact ccaaggcagg agcatagtct gtgatcggtt tcatagttga tccccagtcg
     6601 cggtttgttt ctactgcttg gttaattccg aaggaaacat tacttgactg atggcgtgct
     6661 cctagctggg caatgacttt accgttagaa acatcaacaa tggtagaagc gacttgcaat
     6721 tcatcgtctg gataggcaac gtattcgtct gtattgtaaa tatcccacag atgtttttga
     6781 gcttcttggt ctacatttgt gtagacatcc atcccagttg tgagcaggtt atagcctgtt
     6841 tcttcttcaa cttgattgat gacttccttg aggtaattat ccatgtaagc agggtaatta
     6901 cttgctgatt tgagactttg tagtccatca gtaattggtg tattgattgc tttctcatac
     6961 tgttcagcag agatgtagcc ttgatttttc atttcagata agaccaagtt tcggcggtct
     7021 tgggctgctt ctggatgtga ataggggtca tattggtttg gtgcctgagg cattccagcc
     7081 agcaaggcta actgaggtaa acttaaatta ttgaggtctt taccatagta gttttgagct
     7141 gctgtctgca ttccatagtt cccattagac atgtagacct tatttatata gtaggtcaag
     7201 atttcttgct tggttgcttt ttgttctaac tgaatcgcta accaagcttc ctgagcctta
     7261 cgagaaatag tctggtcgga agtcgaagtt gaaaagtaag tcaacttaat caactgttgg
     7321 gtgagagttg atccaccttg gagggaattg ctttgcagat tgcgcaagaa agctcccagg
     7381 atacggatgg tatcaatccc cctgtggtcg aagaagcgat ggtcttcgat agaaacgatt
     7441 gccttaacca aatctgtggg aatatcatta gcttgggcat tgacgcggcg ttcagaaccc
     7501 aagtcagcaa tgagttgatt tttattgtcg tagattttac tagaagttgt tgcaactagt
     7561 ttactctcgg ataggctagg agccttgcta acgtagtaga aaaaaactcc tccgcctaag
     7621 acaatggctg cgataaccaa gcttaagaag ctaatgctca gatacttgat taggcgcaga
     7681 atcgttggtt tgttcatctt gttttaccac ctaataaatg ttctttgata acattgagat
     7741 aaggaatttg agggaaggca ccagccttga tttcatatcc atattctcga atatattcaa
     7801 gtggcattga tttttgtccc ttatcttgat gatagaagcg aatcaaatcg aatgccggca
     7861 ataagtaggt ttcttgctga gaagaaaagt gaagaaggac aaagcagatt ccttgttggg
     7921 caaggacttg ttccatatgc tgaatctgat gtggatgaaa atttttcatc ggaatcgcac
     7981 gtttttgttt tgtttccttg acttcaaagt cgatgtaata tccattataa acgccagaat
     8041 agtccgtcgt tgaagcttgt cgaaaatagg cttcaacaat cttggcacga cttcgttgtg
     8101 gatagtccac ttgtacgatt tgaataggag ttggtttctt atgtataaca gccaagccct
     8161 gagacaaata gtagtcgttg gtagcattga tcatcttttc aaaagacatt cctcgatttg
     8221 cgaaattttt gggttgagaa agagatgttt gtcttttttg tgatgaaact ttatgtggat
     8281 agttgaccat aattctcctt attggtacaa taacatcact ttattatacc ataatttgca
     8341 cagaaagggt taaaaatggc tacagctttg gttttaggct attctgcttt tgatttgggt
     8401 ttattttctg ataaggatcc tcgtcttaaa ttgattaaaa aagcgatacg taaagattta
     8461 gaggctatgg cagcagatgg ggtgtcttgg cttgtgttta cagggagttt gggttttgaa
     8521 tattgggttt tagaggttgc tcaggagatg aaaaccgaat acggtttcca gttggccacc
     8581 atttttgctt ttgaaaccca tggggaaaat tggaatgaag gcaatcagat gaaactgagt
     8641 cgctttaagc aggtagactt tgtcaaatat gcctatcctc gctatgaaca caaggggcag
     8701 ttaagagatt accagcagtt tttgctggaa aacacgacta gttcctatct tttttatgat
     8761 gaagaaaatg aaacgaaact agcttatttt taccaaaaga tgaaaaatca agaggactat
     8821 tttataaaga gattaacatt tgatcaatta aatgaacttg ctgaaaattt ttccgaaaat
     8881 tgaagctttg accttgattt ttgtttgcct ttttttatat aataatacta gcaagcgaga
     8941 atggagagag acatggcaag tattattttt tcagcgaaag atatttttga acaagagttt
     9001 ggacgtgaag tccgtggcta taataaagta gaagttgacg agtttttaga cgatgtcatc
     9061 aaggactatg aaacctatgc tgccttggtc aagtcacttc gtcaggaaat tgcggatttg
     9121 aaggaagaat taactcgtaa accgaaacct tcaccagttc aagcagaacc ccttgaagcg
     9181 gcaattacaa gttctatgac gaattttgat attttgaaac gcctgaatag attggaaaaa
     9241 gaagtttttg gtaaacaaat tttagataac tcagattttt aagtagttat ttgagatgtg
     9301 caatttttgg ataatcgcgt gaggagaatt gcttctcatg aggaaagtcc atgctagcac
     9361 aggctgtgat gcctgtagtg tttgtgctag gcgaaaccat aagcctaggg acgagaaatc
     9421 gttacggcag ttgaaatggc taagtccttg gataggccag agtaggcttg aaagtgccac
     9481 agtgacggag tctttctgga aacagagaga gtggaacgcg gtaaacccct caagctagca
     9541 acccaaattt tggtcggggc atggagtacg cggaaacgaa cgtagtattc tgactgctat
     9601 cagctagagc tgttagtggt agacagatga ttatcgaagg aagtggtcct agtcacttct
     9661 ggaacaaaac atggcttata gaaaattgca tataggttgg ggctgagaaa ttttctcaac
     9721 ctcatttttt aaagtggaca tatagaaagg tcttgcaaga ctgtaacatg aaaaaaaaat
     9781 ttaatttaat tgcaactgtg gcagcagggc ttgaggctgt cgttggtcgt gaagtgcgag
     9841 agttgggcta cgattgtcag gttgaaaatg gacgtgttcg ttttcaagga gacgtgagag
     9901 ctattatcga aaccaacctt tggcttcggg cagcagatcg tatcaaaatt atcgtaggaa
     9961 cgttcccagc taagactttt gaagagctat ttcagggagt tttcgctttg gattgggaaa
    10021 attatttacc acttggagct cggttcccga tttcaaaagc taaatgtgtt aagtccaaac
    10081 ttcacaatga gcccagtgtt caggctattt ctaagaaagc tgttgtcaag aaattgcaga
    10141 aacactatgc tcgcccagaa ggggttcctc tgatggagaa tggcccagag tttaagattg
    10201 aggtctctat tctcaaagat gtggcaactg tcatgattga tacgaccggg tctagcctct
    10261 ttaaacgtgg ttatcgtacc gaaaaaggtg gtgctcctat caaggaaaat atggcagcag
    10321 ccattttaca actttctaac tggtatccag acaagccttt gattgatccg acctgtggtt
    10381 cggggacttt ctgtattgag gcagttatga ttgctagaaa gatggcgcca ggtcttcgtc
    10441 gctcttttgc atttgaggaa tggaactgga tcagcgatcg cttgattcaa gaagtgcgca
    10501 cagaagcgac taaaaaagta gaccgtgagc ttgagctgga tatcatgggt tgtgatattg
    10561 atgctcgcat ggtggaaatt gctaaggcca atgctcaggc agctggtgtt gcaggagaca
    10621 ttacttttaa gcagatgcgc gtgcaggatt tacgttccga taaaatcaat ggagtaatca
    10681 tttccaatcc gccttatggt gaacgtttgt cagatgatgc aggggtgacc aagctctatg
    10741 ctgagatggg gcaagtattt gcaccgctga aaacttggag caaatttatc ctgactagtg
    10801 atgaagcttt tgaaagcaag tatggtagcc aagcagataa gaagcgtaag ttatacaacg
    10861 gaaccttgaa agtggatcta tatcaatatt ttggtcagcg tgtcaaacgg caagaggtaa
    10921 aatagaaagg gatactcatg agtaaaaaaa gacgaaatcg tcataaaaaa gaaggtcaag
    10981 aaccgcaatt tgattttgat gaagcaaaag agctaacagt tggtcaagct attcgtaaaa
    11041 atgaagaagt ggaagcagga gtcttgcctg aggattccat tttggacaag tatgttaagc
    11101 aacacagaga tgaaattgag gcggataagt ttgcgactcg tcaatacaaa aaagaggagt
    11161 tcgttgaaac tcagagtctg gatgatttaa ttcaagagat gcgtgaggct gtagagaagt
    11221 cagaagcttc ttcggaggaa gttccatctt ctgaagacat cttactaccc ttgcctctgg
    11281 acgatgagga gcaaggcttg gatcctctat tgctagatga tgaaaatcca acagaaatga
    11341 ctgaagaagt ggaagaggag caaaaccttt ctcgtctgga tcaagaggac tcagaaaaga
    11401 aaagtaaaaa aggctttatt ttgaccgttt tggcgcttgt atcagtaatt atttgtgtca
    11461 gtgcttatta tgtctaccgt caagtggctc gttcgaccaa ggaaattgaa acttctcaat
    11521 caactacagc caatcaatcg gatgtggatg attttaatac actttatgac gccttttaca
    11581 cagatagcaa taaaacggct ttgaaaaata gccagtttga taaactgaat caactcaaga
    11641 ctttacttga taagctggaa ggtagtcgtg aacatacgct tgccaaatct aaatatgata
    11701 gtctagcaac gcaaatcaag gctattcaag atgtcaatgc acaatttgaa aaatcagcta
    11761 ttgtggatgg tgtgttggat accaatgcca aagccaaatc ggatgctaaa tttacagata
    11821 ttaaaactgg aaatacggag cttgataaag tgctagataa ggctatcagt cttggtaaga
    11881 gccagcaaac aagtacttct agctcaagtt taagtcaaac tagcagctca agttcaagtc
    11941 aagcaagttc aaatacgaca agtgagccaa aaccaagtag ttcaaatgag actagaagta
    12001 gtcgcagtga agtcaatatg ggtctctcga gtgcaggggt tgctgttcaa agaagtgcca
    12061 gtcgtgttgc ctataatcag tctgctattg atgatagtaa taactctgcc tgggattttg
    12121 cggatggtgt cttggaacaa attctagcga cttcacgttc acgtggctat atcactggag
    12181 accaatatat ccttgaacgt gtcaatatcg ttaacggcaa tggttattac aacctctata
    12241 agccagatgg aacctatctc tttaccctta actgtaagac aggctacttt gtcggaaatg
    12301 gcgctggtca tgcggatgac ttggactact aagcagtcgt tacaaaattc tttcttttca
    12361 aaagtaaaaa tgataaaata aaacaaatga aacaagagga gtgtcaaatg acaaaagcta
    12421 actttggtgt cgtaggtatg gccgtaatgg gtcgtaacct tgcccttaat attgaatcac
    12481 gtggttacac agttgctatc tacaaccgta gtaaagaaaa aacggaagat gtgattgctt
    12541 gccatcctga aaagaatttt gtaccaagct atgacgttga aagttttgta aactcaatcg
    12601 aaaaacctcg tcgtatcatg ctgatggttc aagctggacc tggtacagat gctactatcc
    12661 aagcccttct tccacacctt gacaaaggtg atatcttgat tgacggagga aacactttct
    12721 acaaagatac catccgtcgt aatgaagaat tggcaaactc aggtatcaac tttatcggta
    12781 ctggagtttc tggtggtgaa aaaggtgccc ttgaaggtcc ttctatcatg cctggtggac
    12841 aaaaagaggc ctacgaattg gttgcggatg ttcttgaaga aatctcagct aaagcaccag
    12901 aagatggcaa accatgtgtg acttacatcg gccctgatgg agctggtcac tatgtgaaaa
    12961 tggttcacaa tggtattgag tacggtgata tgcaattgat cgcagaaagc tatgacttga
    13021 tgcaacactt gctaggcctt tctgcagagg atatggctga aatctttact gagtggaaca
    13081 agggtgaatt agacagttat ttgatcgaaa tcacagctga tatcttgagc cgtaaagacg
    13141 atgaaggcca agatggacca atcgtagact acatccttga tgctgcaggt aacaagggaa
    13201 ctggtaaatg gactagccaa tcatctcttg accttggtgt accattgtca ctgattactg
    13261 agtcagtgtt tgcacgctac atttcaactt acaaagaaga acgtgtacat gctagcaagg
    13321 tgcttccaaa accagctgcc ttcaactttg aaggagacaa ggctgaattg attgaaaaaa
    13381 tccgtcaagc cctttacttc tcaaaaatca tttcatacgc acaaggattt gctcaattgc
    13441 gtgtagcctc taaagaaaac aactggaact tgccatttgc agatatcgca tctatctggc
    13501 gtgatggctg tatcatccgt tctcgtttct tgcaaaagat tacagatgct tacaaccgcg
    13561 atgcagatct tgccaacctt cttttggacg agtacttctt ggatgttact gctaagtatc
    13621 aacaagcagt gcgtgatatc gtagctcttg cggttcaagc tggtgtgcca gtgccaactt
    13681 tctcagcagc tattacttac tttgatagct accgttcagc tgaccttccg gctaacttga
    13741 tccaagcaca acgtgactac tttggtgccc acacttacca acgtaaagac aaagaaggaa
    13801 ccttccacta ctcttggtat gacgaaaaat aagtaggtca gccatgggga aacggatttt
    13861 attacttgag aaagaacgaa atctagctca ttttttaagt ttggaactcc agaaagagca
    13921 gtatcgggtt gatctggtag aggaggggca aaaagccctc tccatggctc ttcagacaga
    13981 ctatgatttg atgttattga acgttaatct gggagatatg atggctcagg attttgcaga
    14041 aaaattgagt cgaactaaac ctgcctcagt catcatgatt ttagatcatt gggaagactt
    14101 gcaagaagag ctggaagttg ttcagcgttt tgcagtttca tacatttata agccagtcct
    14161 tatcgaaaat ctggtagcgc gtatttcggc gatcttccga ggtcgggact tcattgatca
    14221 acactgcagt ctgatgaaag ttccaaggac ctaccgcaat cttaggatag atgttgaaca
    14281 tcacacggtt tatcgtggtg aagagatgat tgctctgacc cgccgtgagt atgacctttt
    14341 ggcgacactt atgggaagca agaaagtatt gactcgtgag caattgttgg aaagtgtttg
    14401 gaagtatgaa agtgcgaccg agacaaatat cgtagatgtc tatatccgct atctacggag
    14461 caagcttgat gttaaaggcc aaaaaagcta cattaaaacc gtgcgtggtg ttggatatac
    14521 catgcaagaa tagataagca gttgcagttg tgtaactgct tttttgagga gtttttatat
    14581 attgacatgc agtcgggtct ttgctacaat cgttttagga ggatatatag atgaaacttt
    14641 tgaaaaaaat gatgcaagtt ctactagcag tctttttctt tggtttgcta gctacaaata
    14701 cggtatttgc gaataccaca ggtggccgat ttgttgataa ggataataga aaatattatg
    14761 taaaagatga tcataaagca atctattggc ataaaataga cggtaaaact tactattttg
    14821 gtgatattgg agagatggtt gtcggttggc aatacttaga aattcctgga acaggttatc
    14881 gtgataattt attcgataac caaccagtta atgaaattgg ccttcaggag aagtggtact
    14941 attttggaca agatggtgct ttgctagaac aaacagataa acaagtacta gaggcaaaaa
    15001 cgtctgaaaa tacaggaaaa gtatacggtg aacaatatcc tctatctgct gaaaagagaa
    15061 cttattattt tgataataat tatgctgtaa agacaggctg gatttatgaa gagggtcatt
    15121 ggtattattt aaataagcta ggaaattttg gcgatgattc ttacaatcca ctaccaattg
    15181 gtgaagttgc taagggttgg actcaagatt ttcatgttac tattgacatt gatagaagca
    15241 aacctgctcc atggtactac ctagatgctt caggtaagat gcttacagat tggcaaaaag
    15301 taaacggaaa atggtattat tttggctcct ctggttctat ggcaacaggt tggaaatatg
    15361 tacgaggcaa atggtattac ttagataata aaaatggtga tatgaaaaca ggatggcaat
    15421 accttggtaa caagtggtac tacctccgtt catcaggagc tatggtaact ggctggtatc
    15481 aagatggttc aacttggtac tatttagatc cttctaatgg agatatgaaa ataggttgga
    15541 caaaagtaaa tggaaaatgg tattatctca attcaaatgg agcaatggtt acaggtagtc
    15601 aaactatcga tggtaaagtt tataacttcg cttcatctgg tgagtggatt taatgttgga
    15661 ggatatataa aatgaagctt ttgaaaaaaa ctatgcaagc tggactaaca gtaattttct
    15721 ttggtttgct agctacgaat acagtatttg cagatgattc tgaaggatgg cagtttgtcc
    15781 aagaaaacgg tagaacctat tacaaaaagg gggacctcaa agaaacctac tggcgagtga
    15841 ttgatggcaa gtactattat tttgattctc tatctggaga gatggttgtc ggctggcaat
    15901 atatcccgtt tccatctaaa ggtagtacaa ttggtcctta cccaaatggt atgagattag
    15961 aaggttttcc aaagtcagag tggtactact tcgataaaaa tggagtgcta caagagtttg
    16021 ttggttggaa aacattagaa attaaaacta aagacagtgt tggaagaaag tacggggaaa
    16081 aacgtgaaga ttcagaagat aaagaagaga agcgttatta tacgaactat tactttaatc
    16141 aaaatcattc tttagagaca ggttggcttt atgatcagtc taactggtat tatctagcta
    16201 agacggaaat taatggagaa aactaccttg gtggtgaaag acgtgcgggg tggataaacg
    16261 atgattcgac ttggtactat ttagattcaa caactggtat tatgcaaaca ggttggcaat
    16321 atctaggtaa taactggtac tacctccgtt cctcaggagc aatggccact ggctggtatc
    16381 aggaaggtac cacttggtat tatttagacc agccaaatgg cgatatgaaa acaggttggc
    16441 aaaaccttgg gaacaaatgg tactatctcc gttcctcagg agctatggca actggttggt
    16501 atcaagatgg ttcaacttgg tactacctaa atgcaggtaa tggagacatg aagacaggtt
    16561 ggttccaggt caatggcaac tggtactatg cttatagctc aggtgctttg gcagtgaata
    16621 cgaccgtaga tggctactac gtcaactata atggcgaatg gagccggtaa tgacagggcg
    16681 cttataatta tattagaaca ctcgcttaat caaagaagtg actttgttgt acttgacctg
    16741 acgattgggg agattaaaag catgtgaaat ctacacatgc tttttcgtta gttatgagtg
    16801 ttgaacttga ctaaccattg acaaaagata agagcaatca aaacaagata caaaaatatc
    16861 aatgctaact gcaccgaaat gcccgatgct atggataggg ctaataggga gagcagggac
    16921 ggggcgagaa tgtccactgt gctcagagca gagcccaccg ttcccatctt gtcactgggg
    16981 atatgggcaa agacagctgc ttgtaggcga gggctgagaa tgccgataat cgtagaactg
    17041 atgaaactgg tcatgaaaat tacgatatag gcttgacaaa ggatactagt tattagagcc
    17101 aatagggaaa tctcacagaa aaccaagagg tgcatgaaac ggatattttt aaatagatta
    17161 ctgctggtca tattgccaag gatagctcca caagacatga tgatggagag cagggcgaag
    17221 agttgcccaa tgctcagttg tgctagtgtc tgttggatga agcggagggc aatcagccga
    17281 ggggacacat ccaagatggc aatcatactg gtcgtcagaa acagtagtat gacggtatct
    17341 tttaacctca ggagcacagc cagcgactgt ttaagatgtt gaaaatattt cttaaaactc
    17401 agtgctgtat gtgacatttc aatccttttt tcaacctcat acaaggtatg tcgaataaca
    17461 tacaggcaca aaaaggcaat gacaaaagtc agagtgttga taagggaaat agtttgaata
    17521 cttataacat tgataaggaa tgcgccagcc agattggcga caatgtggac aaccctcatc
    17581 agagactgcc tgaaccccat ggcatcatgc aggtcatcct taattacact gatgtagagg
    17641 gcgttcatca tgtaggcaga caggtagctc aaggtgtcag agatgaggtt gatgatgaca
    17701 atcataatga ctgaaactaa tgaaaagggc gacaagacga gaaatactgt caaaatagcg
    17761 tagagcagga ttttgatttt ggcaatacag agtgcgtgtt tgactctatt ttcttgaaaa
    17821 tctcctagaa tcccgagaaa gagttgcgat agtaggggaa ccatctctga aatggaaatc
    17881 agtaaaatcg ctagcggagc gaaattgtac tgagccacat aattgataat ggcgaggtag
    17941 aaaatactgt cgccgaatga tgaaaaacag ctgtttagca gcaactgtct atatatgttg
    18001 tttgtccaaa ataatttcat acatttctcc ttatgtctgc acatcgaatg ggaatttatt
    18061 atttaataga caccaatata ccccatgctt cctattccga agtcaaacaa atctgcttta
    18121 ccactatctc cttgaaacat cagtaaaccg tgatcatctc gttcagaaca tgctaaaatt
    18181 acctgttgga tttttttcat cagtaatttg ttttgattgt agacagtggt ttgtagtaag
    18241 ctagttattc cgtggcaata tcctgtcatc atttttagat gatggttaaa aacaaattct
    18301 tgatatttac taataatatc tttgtttccg tcacaatcgt acatacaaag atataagatt
    18361 attccggaaa ttccttcgca ccaagaaagt tgtaaatttc ctaaattttc agtattagaa
    18421 gtaacttttt ctaataattt ttttaattca gtatggaatg tatggagatc attatagaac
    18481 atagaaggtt ctaagacttt agaataggca aataaagcat atgcaattcc acaatatcca
    18541 tgagcaaagc tatagtctaa aatgctttct ttggctgtat ctctttgtag gaaataatga
    18601 taaatttccc catatactat tgtggatgaa agt
//
LOCUS       DAVLSL010000027        18607 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLSL010000027 DAVLSL010000000
VERSION     DAVLSL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.027"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            163..834
                     /gene="sdaAB"
                     /locus_tag="VXN30_000731"
     CDS             163..834
                     /gene="sdaAB"
                     /locus_tag="VXN30_000731"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1357392.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            843..1715
                     /gene="sdaAA"
                     /locus_tag="VXN30_000732"
     CDS             843..1715
                     /gene="sdaAA"
                     /locus_tag="VXN30_000732"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1357393.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            1737..2369
                     /locus_tag="VXN30_000733"
     CDS             1737..2369
                     /locus_tag="VXN30_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1357394.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            2419..2676
                     /locus_tag="VXN30_000734"
     CDS             2419..2676
                     /locus_tag="VXN30_000734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357395.1"
                     /translation="MKIKEQTRKLAAGSSKHCFKVVDGTDEVSSKHCFKVVDGTDEVS
                     SKHCFKVVDGTDEVSSKHCFEVVDRTDEVSNHIRQGDVDVV"
     gene            complement(2762..4612)
                     /locus_tag="VXN30_000735"
     CDS             complement(2762..4612)
                     /locus_tag="VXN30_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1357396.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(4654..5811)
                     /locus_tag="VXN30_000736"
     CDS             complement(4654..5811)
                     /locus_tag="VXN30_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1357397.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINQNML"
     gene            5869..7035
                     /locus_tag="VXN30_000737"
     CDS             5869..7035
                     /locus_tag="VXN30_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1357398.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            7174..7500
                     /locus_tag="VXN30_000738"
     CDS             7174..7500
                     /locus_tag="VXN30_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1357399.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            7487..8080
                     /locus_tag="VXN30_000739"
     CDS             7487..8080
                     /locus_tag="VXN30_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1357400.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            8073..8984
                     /locus_tag="VXN30_000740"
     CDS             8073..8984
                     /locus_tag="VXN30_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1357401.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTDNHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            9046..10110
                     /locus_tag="VXN30_000741"
     CDS             9046..10110
                     /locus_tag="VXN30_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357402.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEVGEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(10288..11148)
                     /locus_tag="VXN30_000742"
     CDS             complement(10288..11148)
                     /locus_tag="VXN30_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1357403.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(11270..12256)
                     /locus_tag="VXN30_000743"
     CDS             complement(11270..12256)
                     /locus_tag="VXN30_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1357404.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(12528..14003)
                     /locus_tag="VXN30_000744"
     CDS             complement(12528..14003)
                     /locus_tag="VXN30_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1357405.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKATDSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(14261..15184)
                     /locus_tag="VXN30_000745"
     CDS             complement(14261..15184)
                     /locus_tag="VXN30_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1357406.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(15198..16127)
                     /locus_tag="VXN30_000746"
     CDS             complement(15198..16127)
                     /locus_tag="VXN30_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1357407.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(16446..16796)
                     /locus_tag="VXN30_000747"
     CDS             complement(16446..16796)
                     /locus_tag="VXN30_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357408.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRNILQIVKDAI
                     VSLESIKESQSATKDGGQTVGKETVDKDVLENSQEELQKAVLFLADEKGSEHTAAELI
                     DNLKEVIAKLKANA"
     gene            complement(17069..17392)
                     /gene="tnpB"
                     /locus_tag="VXN30_000748"
     CDS             complement(17069..17392)
                     /gene="tnpB"
                     /locus_tag="VXN30_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1357409.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
     gene            complement(17653..17952)
                     /locus_tag="VXN30_000749"
     CDS             complement(17653..17952)
                     /locus_tag="VXN30_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1357410.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(18117..18524)
                     /locus_tag="VXN30_000750"
     CDS             complement(18117..18524)
                     /locus_tag="VXN30_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1357411.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
BASE COUNT         5538 a         3940 c         3523 g         5606 t
ORIGIN      
        1 attacaaaat tattttttat ttccctaaat aaggtgtttt ttgtcataaa agatactatt
       61 tttatgctat ttgaggtatt agaaaggttt tcatccacaa ttaaagccaa gcccctcatt
      121 ctttgatata atagagataa gaatttttat aaggggaaac tgatgaaatc acttcgtttt
      181 caatctgtct ttgatatcat cggaccagtt atgattggcc catctagtag ccataccgct
      241 ggtgctgttc gtattgggaa gattgtctct tccatttttg atgatactcc gacagaagtt
      301 gaattccaac tttttaactc atttgccaag acctatcgtg gtcacggaac agacctagcc
      361 cttgttgcag gtattttagg aatggataca gatgatcctg aaattccaaa tagtctggaa
      421 attgcccaca agcgtggtat caagattgtc tggaccattc agaaagacag caatgcccct
      481 caccctaaca ccactaaaat tactgttaaa aatgcccaca agaccatcag cgtgactggt
      541 atttctatcg gtggaggaaa tattcaggta accgaactca atggttttgc cgtctctctc
      601 aatatgaata caccgactat catcatcgtt catcaagata ttccaggtat gattgccctt
      661 gttacagagg cgctttcccg ctatggtatc aatatcgccc agatgaatgt cactcgtgaa
      721 aaagctggtg aaaaagccat tatgattatc gaagttgaca gtcgcaactg tgatgaggct
      781 atcgaagaaa ttcgaaaaat ccctcatctc cacaatgtca atttctttaa ataggaggaa
      841 gcatgtttta ttctatcaaa gaattggtcg agcaagcaga tctggacttt caaggaaatg
      901 tcgcagaact catgattaca acagagtttg aattgaccgg tcgcgaacgt gaagaagtct
      961 tccttctcat ggaacgcaat ctggaagtta tgaaagcctc tgtccaactt ggcctcaatg
     1021 aaaataaatc tcgtagtggc ctgacaggtg gagatgctgc caaattggat cactacatcg
     1081 aaaacggaaa aactctgtca gattatacga ttctctcggc tgcccgaaat gccatcgcag
     1141 tcaatgaaca caatgccaaa atgggcttgg tctgtgccac tccaaccgct ggaagtgctg
     1201 gctgtctgcc atccgttctc actgctgcta ttgaaaaatt agacctcagc cacgagcaac
     1261 aattggattt cctctttgct gctggtgcct ttggactagt catcgcaaac aatgcctcca
     1321 tctcaggtgc tgagggtggg tgtcaagctg aagttggttc agcctctgct atgagtgctg
     1381 ccgccttgac tctggctgca ggtggaacac cttatcaggc cagtcaagct attgcctttg
     1441 tcattaaaaa tatgctaggc ctcatctgtg accctgttgc aggtttggtc gaagttccct
     1501 gtgtcaaacg taatgccatg ggagctagct ttgctttcat cgcagcagac atggccttgg
     1561 caggtatcga atctaaaatc cctgtggatg aagtgatcga tgccatgtac caagtaggag
     1621 caagcatgcc aactgccttt cgtgaaacag ctgaaggtgg actcgctacc acccctactg
     1681 gtcgtcgcct ccaaaaagaa attttcggag aataagctta tcaaatagga gaaaccatga
     1741 cctctatcac agcgattttt ttcgatttgg atggaaccct cgttgatagt tctatcggga
     1801 ttcacaatgc ctttacctat acctttaaag agctgggggt gcctagccct gatgccaaaa
     1861 ctattcgtgg ttttatggga ccacctctcg aaagtagttt tgcgacctgc ctgtccaaag
     1921 accaaatttc tgaagccgtg cagatatatc gttcttacta taaggcaaaa ggcatctatg
     1981 aagctcaact ctttcctcag attatagact tgcttgagga gttatcgagc agttatccac
     2041 tctacatcac cacgacaaag gatacatcta ctgctcaaga catggcaaaa aacttggaaa
     2101 tccatcattt ctttgatggc atttatggtt ctagccctga agcaccccat aaggcagatg
     2161 tcattcacca agccttgcag acacatcaac tagcaccaga acaagccatc atcatcggag
     2221 ataccaagtt tgatatgctg ggagctcgag aaacaggtat tcagaaattg gccatcactt
     2281 ggggatttgg agagcaagca gatctactaa actatcaacc tgattatatc gctcacaaac
     2341 ccttagaagt tttggcgtat tttcaatagc atagactggg taacaattcc atttcagaag
     2401 aaaatgaagt aatactcaat gaaaatcaaa gagcaaacta ggaaactagc tgcgggcagc
     2461 tcaaaacact gttttaaggt tgtagatgga actgacgaag tcagctcaaa acactgtttt
     2521 aaggttgtgg atggaactga cgaagtcagc tcaaaacact gttttaaggt tgtggatgga
     2581 actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagt
     2641 aaccatatac ggcaaggcga cgttgacgtg gtttgaagag attttcgaag agtataagtc
     2701 tctacataat aaaacgcata ttaccaaggt tgtgtaactt gatactatgc gtttttcttt
     2761 tttattcaat gtgggttgtt tgattagcaa aggcgataat agcttgcttc aactcatctc
     2821 cactgagagt gcggaactct ccaatctttt gattgatgga ttctaaaggc atgacattaa
     2881 ccttaccaac gaaaatctta tccataactt gattatcaac gagttcggtt gataccaaga
     2941 gttcttcgta gagtttttca cctgggcgga ttccaacttc aacgattgga atttcacttt
     3001 cagtgtggcc acttagaagc accatcttct tggccaagtc ataaatcttg actggtttgc
     3061 ccatatcaag gataaagact tccccatctt tggcataagc accagcatgg ataaccagac
     3121 ggctagcttc tggaatggtc ataaagtaac gggtcatgcg gaagtctgtc accgttacag
     3181 gcccaccttc agcaatctga cgttcaaaga ctggaatgac actaccacgg ctaccaagaa
     3241 cattcccaaa acgaactgca cagtaggttg attggctacg ttggttaaag ccagtgacaa
     3301 tcaactccgc cacgcgcttg gttgctccca taacatttgg tggattgact gccttatctg
     3361 tcgaaatcat aaccatctta gacactttag cttcatcaac agccttagca acattgtaag
     3421 ttccacggat attgtttttg aaggcttctt ttggattgcg ctccatcata ggaacatgct
     3481 tgtgggctgc cgcatgataa acaatagcag gtttgtactg ctcaaagact tgcaacaaac
     3541 gatcatagtc ttgaatgtcc gcaatcacag gtacataatc aatcccttgg aacttacgaa
     3601 tcaattcatg ataaacaagg tagattgagt tttccccatg accgagcaag acaatgcgtt
     3661 caggattgaa gcgactaact tgacgacaga tttcagaacc gattgaacct ccagctcctg
     3721 tgactaagat ggtcttacct gtcagttctg cacccagacg cgattcgtca agacggattt
     3781 cctgacgacc caaaaggtcc gtaatatcaa ttttttggaa gccagtacct gcttggtgaa
     3841 ggccctgaac aacagtttca accttaggca tcttgtaaca tttgacaccc agcttattac
     3901 acatctgcaa gatacgctca tattctgacg gatccagcga cggaatcgca acgatgacac
     3961 gctcgatttg atggcgtttg gctaattcag gcagattgtc ataagagccc aaaacaggaa
     4021 taccaccaag tttttgaccc tttttcttag aatccttatc caaaataccg accagttcta
     4081 attcactggt tggatgttgg taactatcca taaaaagagc cccaccatca ccggcaccaa
     4141 tcaagaaggt ccgacggtgt tctccatcac cactaccttt tttgcgtctg gagtagatta
     4201 actgccaagt aatccgtggc aataaaatca agaaggtact caacaagata aagagaatga
     4261 tgaaacggat ggagaagagt ggcaagaagg cataacagat actatatgac aagacactgc
     4321 tagcagtcac accaaaaaag attttcatga aatccgtaat cttgctgtaa cgactaatgc
     4381 tcgcgttcaa cccccaaaaa ccaatcatca attgatagaa caggaaggcc aaactcgtat
     4441 agataatgta gtcaacaggt gctggattaa tcagcccata aaataaaata taagatacaa
     4501 tgatggaaac caccatactg aaaatatcaa atattcccca gaaaacctgc ttttgttgtt
     4561 tatttaaaat ttccaccaga tcaatcacat aatctgttag ttttttattc atagaaatct
     4621 actcctcctt aaaagagttt tactatattg ttattatagc atattttgat ttatttggct
     4681 tattttatcc aaataaatta ttgttctctt gtactccttc ttccatttaa atcctctaat
     4741 taaacctaca atcgtcccaa ggccataagc aaagtgaatg gaaaataaaa gaaagggcat
     4801 cacaattaga aatccatttt tatgttttaa taaagtcagc aaagtgagta atgacaaaag
     4861 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     4921 actaaacaca agactcaaaa caaataaaca aggaacatag tgaaataatg ataaacactt
     4981 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     5041 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     5101 ataaccatat tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     5161 atttactaaa ccaaccttct ggaaaacctc tcgtttatac attccatgaa aaatagaaga
     5221 aacatatcta tcctcagaac tatttcgata attggcaatg ctactgccaa acatattttc
     5281 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct tcgacaatcg tcggtctagg
     5341 ccccccacag acaaattcac cttgttgaat aatagccaca ttgttcatta caaaagtctc
     5401 agtaactttt gaatgagcat caatttttaa aataaggtcc cctacagaat gtttaactcc
     5461 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     5521 ctctgtatct tcctttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     5581 atttataaat agaatttcaa tattctcttt aggataggtt tgatttttta agtcttcaat
     5641 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     5701 ttcaacattt tgagtcacta ctactctcct gaatttttcc ttccaccatt ctatcataat
     5761 tttcaagact ttcccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     5821 aaagccttaa aataagctgt tattaaaatc agctagaaga ggtattatat gaaaaagcaa
     5881 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     5941 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     6001 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccaa
     6061 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     6121 ggctcactta ttcgactaat caatctgccc ctgctctatc taggaacctt gatggttggg
     6181 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     6241 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     6301 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat ccttctcctc
     6361 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     6421 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     6481 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgtcat gacctggtta
     6541 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     6601 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     6661 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     6721 atgctctttt tcccaatcaa taatttcatt tactggctta ccatccatct cctcatcgga
     6781 accgcaacca gcgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     6841 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     6901 tttgggccaa ccctctttgg ttacagtttt gacctgttcc actcttgggt accagctgta
     6961 gctgccctct tgctcatcta tatcctgatg actgtggccc tctttacagt ggacagagcg
     7021 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     7081 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     7141 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     7201 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     7261 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     7321 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     7381 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatgggca
     7441 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     7501 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     7561 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     7621 cgctagtttg ggaactccca aagaagcggc ccacgaagtt ctatccaatc ttctcgataa
     7681 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     7741 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     7801 cgcaatcctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     7861 tatcatcggc ggattcctat ttttagttga aagtttcact atcctcgccc aagccaaatc
     7921 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     7981 acttggcatt tcctatgtag ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     8041 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     8101 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     8161 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     8221 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     8281 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     8341 accatgattt tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     8401 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     8461 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     8521 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     8581 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     8641 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     8701 cagataatca cttccacgct gaaaatatcc aagtccatgg taaggttgaa ctgactgcca
     8761 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     8821 caagtaacta cggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     8881 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     8941 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatc
     9001 tgctactatg gaagcatatt tatctaacca aaaggaggtt ctaccatgaa acaagaatgg
     9061 tttgaaagta atgattttgt aaaaacaaca agcaagaaca agcctgaaga gcaagctcaa
     9121 gaggttgcag acaaggctga agaaacgata gccgatttcg atacaccaat tggaaaaaat
     9181 actcaagtag aggaggaagt ctctcaagct gaagtcgaac tggaaagcca gcaagaagag
     9241 aaaattgaaa ctcctgaagt cggtgaagcg agaacagaaa tagaagaaaa gaaggcatct
     9301 aattctactg aagaagagtc agacctttct aaagaaacag aaaaagtcac tatagctgaa
     9361 gagagccaag aagctcttcc tcagcaaaaa gcaaccacga aagagccgct tcttatcagt
     9421 aaatctttag aaagtcctta tatccccgac caagctccaa aatctaggga taaatggaaa
     9481 gagcaagtgc ttgatttttg gtcttggcta gtggaagcga tcaaatctcc tacaagtaag
     9541 tttgaaacaa gtatcacaca cagttacaca gcctttctct tgctcattct gttttctgca
     9601 tcttcctttt tctttagtat ctatcacatc aaacatgctt actatggaca tatagcaagc
     9661 attaacagtc gcttccctga gcagctagct cctttaactc ttttttctat catctctatc
     9721 ctagtagcga caacactctt cttcttttca ttcctcttgg gtagtttcgt tgtgagacga
     9781 tttatccacc aggaaaagga ctggacacta gacaaggttc tccaacaata tagtcaactc
     9841 ttggcaattc caatcttcct cactgctatt gctagtttct ttgctttctt tgatagccta
     9901 cgatttacag ccctcttgtg tgtgattagc attggaatca ttctgcttgc cagtctccat
     9961 atcattacaa gaccaagtca agcaagtgaa accgactcct tctatcaatt attcttgtct
    10021 gtccttgtga acggagttat tatcctcctc ttctttgtag ctgaagtggc actgattgga
    10081 gattatcttc gtatcttggc ctttctttaa acttcatact cttcgaaaat caaattcaaa
    10141 ccacgtcagc ttcgccttac cgtactcaag tacagcctgc ggctagcttc ctagtttgct
    10201 ctttgatttt cattgagtag caatcctgtc atccttggca ggattttttc tatgtcaaaa
    10261 aagcaagtcg attgacctgc ttctgcttta cttttcttgt gctaatgttt taaaatcttg
    10321 tcctaggatg ggttgtattt ctatttgatt ggcgtctagt tggtggtaaa aagatggggg
    10381 taatacctct aagaattttg catagtccaa gcgagctatg gattcatagt cttctggttt
    10441 agagccgtct cctgtgattt gaaccaagta aatctcccac tggacttcct tatccaccaa
    10501 ttgctcaata taggctgcag gaacaaacgg ttctctcggt aaaactgtct tgactccttg
    10561 agctaaatgg taaacgccat gcacttttcc ttctccatcc tgatagaagg aaactcttgc
    10621 aaagtaggca tctgtctctt gaaccgcacg cacacgcgct tcaaactgag ccaagccatc
    10681 tgcctctaaa aagcttttca agttctcacg actaaagaaa ataggattaa aaattccttg
    10741 gcaaatcgta aaacgagcta cagggtctgc tagataggct tgaatacttg cttggaaata
    10801 agcttcaaaa tcaaaaccat ctagcccttc aatatcagtc cctgtataag caagcaaagc
    10861 atctaaaaat gacttgataa tctgccagtc tgtctttgtt agtaggtcag gaagatcgac
    10921 acggtaagcc ttttgaccat cagcataact gaccttaaag agaaattgtg attgccccac
    10981 tatcgcacac tcaatatact cgagacgatt aagaggctga cgaagataga ctacatcata
    11041 gctatgtgac tccaaaccgt ctagcaaggc caagatcgac ttggcattca aaatctcttg
    11101 ttctcctaaa atgctctgtt tatttggaat aaaaaatgtt tttgccataa ctggactcct
    11161 ttgaaatcgt ttacatacag tataccttat tttcctgaaa gatacaaaaa caaactttag
    11221 atttttgagt acaaagcata gaaaaacagc ccctgaggac tgtttgatct tatacagtag
    11281 ctgcctgatc caagctttca ccgatagcgg ctaggcgctc aatcacttca gcttgtgtca
    11341 attcattttt tgaaacatag cggttacgtg ggtgaacacg gcactcgtgt gagcatccac
    11401 gaaggtactt gtcttcattt tcttctgatg tcaagatacg acggttacag aagggatttc
    11461 cacagttgac ataacgttca catggtgttc catcaaacca gtctttccct acgatggttg
    11521 ggttgacatg gttgacatca acggcaatac gctcgtcaaa gacgtacatt ttcccatccc
    11581 aaagctcacc ttgaacttct gggtctttac cgtaagttgc gattcctccg tgcaattggc
    11641 cgacatcttt gtagccttca cggaccatcc agcctgagaa tttctcacag cgaacgccac
    11701 ctgtacagta aaccacgaca cgcttgtcca tgaatttttc cttgttatca cggacccatt
    11761 gtggtaactc acggaagttg cgaatatctg ggcgaatagc cccacggaaa tgtcctaggt
    11821 cgtactcata atcgttacgt gtgtcaagga caacggtatc tttatcaaga agcgcttctt
    11881 tgaactcttt tggagacaag taagcacctg ttgtttcaag tgggttgatg tcattgtcaa
    11941 agtcgttgtc ttccaaacca aggtggacaa tttctttctt gtagcgaaca aacatcttct
    12001 tgaaggcttg ttcattttct tcgtcaatct tgaaccagag atcttccata cctgggaggc
    12061 tgtgaacgta gtccatgtat ttttgagttg tttcatagtc acctgaaact gttccgttaa
    12121 ttccctcgtc agcgactagg atacggcctt taaggccgat tgatttacag aaagccaagt
    12181 ggtctgcagc aaattgctct gcattttcaa ttggagtata aaggtagtaa agtaagacac
    12241 gaatatcttt tgccataaga tttgttctct tttctattct taaattttct aaatttttta
    12301 tttatccata ctagtatacc tgcttgagaa aacgaatgca atttatttga ctggaaattg
    12361 tagaggattc tcgaaatttc ctgaatggtt aaaataagga caagagaaaa cagggatatc
    12421 tatatccttg tgccaaaaaa accactgccc tccccagatc aacctgagga aagcagtgat
    12481 tcttatttta ggagttagga atgaatacac gaaatcaatt tagctgatta ttttttgttt
    12541 ttcaagaatt catcgtattg tttttgcatt tcgttcaata ctttttcgta ggcaccttca
    12601 gatttcaatt tttccatcaa ttctggaatc gctttatctg ggtctacagt accagtgttg
    12661 atagctgtat caaattgttg cattgtgtta gcaatagctg agatttcaga tttcacattg
    12721 tcagtattaa agataaatcc aagcgctgga gattctttag cttctgccaa ttctttctta
    12781 gaattttcga tttgttggtc tgtaacgttt tcgttgatgt aaaggatcca gttgttacca
    12841 gtgttccatc cacccatgtg agtgtttcct ttgtagccat caagaacgcg aacacggttt
    12901 tctttacctt caattttttc ccagttcttg ccttctggac cgtaaacaag accgttcaag
    12961 agttctgggt tcgtattcaa gaggttcaag atttccattg atttttcttt gttcttagag
    13021 ttgtttgaga tgacaaagtt agcaacttgt gttgtttggt ttttcttgat gaagttagta
    13081 attggtttga tttggatatc tttgttggca acacgtgaaa gcaagctgtt accgtagtca
    13141 gctggtccta ctgtttcttc acgaacgaac caagtatctt gttgaaggtc aaaggaagta
    13201 tcgcttgttg cgacgtcttt tggaatgtag ccagcttcat agaatttgtg aagagtcttc
    13261 aagtgttctt tgaaacgagg tacttcgtaa cggtttacaa ctttagtagt atcgccttca
    13321 aggtcaataa cgaatggaag accgtttgct actgggtagt caaagttatc agatgggatg
    13381 aaaactttac caatagcaaa tggtactacg tctggagctt tttctttgat ttgtttcaag
    13441 actggctcaa gagtttcgta agaagtaaca cctgaaatat cgataccata tttggcaagg
    13501 agagttccgt tgaaggcaaa gttttgagat gatgctacgt tagctgcaac tggtacagca
    13561 taaatcttac catttacagt attacccttg atataagctg gatcaagtgc cttgtaaagg
    13621 tctttacctt cttttttgta caattctgtc aagtcagcat aagcaccttt ttgagcattt
    13681 acaatatagt tatctgcaaa ggcgatatca tagttttcac cagatgatgt gataactgac
    13741 attttcttac catagtcacc ccagccaagg tattggatat ccaatttagc accaactttt
    13801 tcttcgatga ttttgttggc atttgctaac aattcatcca agttatctgg tttgtcaccg
    13861 atttggtaca ttttgataac aggtttgtca cctgaatcag tagctttttt gctgttacct
    13921 gtcaaatttc cacaagcagc aagacctgca gccaaagcga ctacactagc agatgcaaaa
    13981 gcatattttt tccagttttt catgatgaaa actccttttt ttatttttaa acttataaac
    14041 aatgtaatga tcttatactc aatgaaaatc aaagagcaaa ctagaaaact agccgcaggt
    14101 tgctcaaaac actgttttga ggttgtggat aagactgacg aagtcagtta catatatcta
    14161 cggcaaggcg acgttgacgc ggtttgaatt tgattttcga agagtattaa cttcacacaa
    14221 gggaagttgg gaactgagaa atgttctttt tcaataagca ctattctttc acaccaccga
    14281 tagtcaaacc ttttacaaag tagcgttgga agaatggata taaaatcgcg attggaaggg
    14341 ttgcaaccac aaccatggcc atacgacctg tttctttcgg tagagcaact cccagttgac
    14401 cagtcaagcc gaccgctttg gcaatgtagt ccatattttg ttggatttgc atgagcaaat
    14461 attgcaatgg atacaagtta tcactcttga tgtaaagaag ggcgttgaac cagtcattcc
    14521 agaaaccaag agctgtcaaa agcgtgatgg ttgcgatacc tggtagtgac aatggcaaac
    14581 agatttggaa gaaaatccgg gcctcactgg caccatcgat acgagccgat tcgagaatgg
    14641 cttctggaat ggtcttcttg aagaaggaac gcatcaagat gatattgaat ggtgagagaa
    14701 gcattggaac aatcaaggcc caaacagtgt caccaagctg aagtacacgg gtcaccatga
    14761 tataacctgg taccaaacca gcgttgaaca acatactgag aaggacgaag atggtaaaaa
    14821 atctgcgata cttaaaggtt gtccgtgaaa tagcgtaggc ataggttgtt gtgataaaga
    14881 catttgtcaa tgtcccaact acggttacaa agacagagat gaagagggct tgtaggattt
    14941 tatccttaaa ctgtgccaaa aactcaaaac cgtctaagcc aaattgggat gggaagaagc
    15001 tatagccgta ttggaggagg cttttctcgt ctgtcactga aataatgata acgaatacaa
    15061 aaggtaggat acaagagagg gcaatcaaac ccgaaatgat actgaagaag atatctgctt
    15121 tcttactgaa ggagtgaatg ccgacattat caattttttc ttttttaatt ttcttttttg
    15181 ccatattctc ctcctttcta gaacaaagct gagtttggat cgactcgtct tgcaagcaag
    15241 tttgatagga taaccagaat caaaccaaca acggattggt aaagaccggc tgctgcagcc
    15301 ataccgatat ctgctgtctg agtcaaacca ttaaaaacat atacgtccaa aacgttggtt
    15361 acattgtaaa gctgaccagc attgtgtggg atttgataga agagaccgaa gtctgcgcgg
    15421 aagatatttc cgactgcaag gatggtcaat acagttacaa gcggagtcaa ctgaggaatg
    15481 gttacgttgc gaatacgttg ccacttgcta gctccgtcca ctgtcgctgc ttcgtagtag
    15541 gttggatcaa ttcccatgat cgtcgcatag tacatgacac tgctatatcc aaaacctttc
    15601 caaataccta ggaaaagtag gagatagggc cagatgccca ggtcagcgta gaaattgact
    15661 tctttgagac caagactttc caatagatga ttgaacaccc ctttatcaat atttaggaag
    15721 gcatctgtaa agaaactgat gataacccaa gacaagaagt aagggaacaa catagaagtt
    15781 tggaaaatct tcaccattct cttagaacgg agctcgctga ggataatggc aatccctaca
    15841 gatacaacta aacctagaaa gataaagcca agattgtaga ggacagtatt tcgtgtgata
    15901 ataaaggcgt ctcttgaact aaataagaat ctgaaattat cgagtccgac ccatttacta
    15961 tttatgatac tatctatgaa accattactg gtcatgtggt agtctttgaa ggcaaccacg
    16021 ttcccaaata ctggaatgta aaagaataga atcaaccaga gtgcccctgg caaaaccatc
    16081 aagagaaaga tccagttgtc tctcaatgtt tttgaaaact ttttcataat ttcctccctt
    16141 tttattttga tatccatcta aaaattcttt tttagacttt tgataacgat tacattatta
    16201 gtatactcct atttgcaggt taggttaaac tcctaattat agaaaaaact ccacaaatta
    16261 tgtagcagat ttaaaacttt atcaccacta tcaaacaaat gtcctaaatc aattgtttat
    16321 tttatctcta ttagcccagt gatggcgtca ctctgttata agcatccaac aacggggtat
    16381 actgaaaaat ctccagacta gggaactcag cgatagttcc taatctggag atttttaata
    16441 tgttattagg cgtttgcttt caacttagca ataacctctt taagattatc aatcaactct
    16501 gctgcagtat gctcagagcc tttttcatct gccaagaaca aaactgcttt ttgaagttct
    16561 tcttgagagt tttcaaggac atccttatct actgtttctt tgcccactgt ttgtcctcca
    16621 tccttagttg ctgactggct ttccttaatg gactctaggg aaacaatggc atctttgact
    16681 atttgcaaga tattacgtaa accttgctct gtcaaactat catctgcaaa agctttctta
    16741 gcctctgcca aaaccagacg tgctgaatct gtggtaggat tcgatacacc tgtcaatgat
    16801 ctcaaaagat tttctaaggt ttgagtctgc ttactaatac tagactaaaa tcaaaaagta
    16861 ttatataaca gtgatatgaa atcaactaaa gaagaaatcc aaaccatcaa aacactttta
    16921 aaagactctc gtacagctaa atatcataaa cgccttcaaa tcgttctata gtaaaatgaa
    16981 acaagaacag gacaaattga tcaggacagt caaatcgatt tctaacaatg ttttagaagt
    17041 aggggtgtac tattctagtt taaatccact atatttgggg agtgatagaa aagcccttca
    17101 taagccagtc tacttgttca ggtgcgagag ctttgacatc cttttctgta ctgggccaag
    17161 tcagttttcc gttctcaaag cgtttatata gtatccaaaa tccttgacca tcccagtaaa
    17221 gggctttaaa gcggtcttta cgtccaccac aaaagagaaa gacttgaccg gagaaaggat
    17281 ccaattcaaa gtgggtttta acgagataag ccagtgaatc gattccttgt atcatatctg
    17341 tcttgccaca aacaaggtga acttgaccta aatcacttag ttgaatcatc atagtacaat
    17401 acttttcctc cgataattat tttttatcta gtatactgga agttggggaa ttaggataga
    17461 taccttgtta tgacgcgctt acgtaacttg taactagctg cctagtttga tctttgcttc
    17521 ttcattgatt agcagcagat ttcaaaatga taaaaacgta taatagtggg tgtagaaact
    17581 cttgctacta tgcgttttat tatggaaaga cttattggac tttctctcaa atcgagtttt
    17641 tactcaattt tcttacttga ttgggattga aattccaatt aatttctctg agtagagtgt
    17701 cttgatattg gcttcatcaa cagaggcctt atcaatttta cgtttcaaga aaaattcttg
    17761 aatggtttcg atttcaggct cacgaatagc acggtgtttg tttgagatga ggatttcata
    17821 gtgaagcgga gcttgggtaa aaataacatc tgtattccct gcagaataaa cctcaacaag
    17881 ggttgcatcg gtactttcta gctgactttt tacaagttgc gagtgtgagt ttgtcgtatt
    17941 gataagcttc ataatatttc ctccgatttt ctaattctat tatagcactt tttgaataaa
    18001 gtcgcttgat ttatactcaa tgaaaataaa agagcaaact aggaagctag ccgcaggcta
    18061 tacttgagta tggtaaggcg acgctgatgt ggtttgaatt ttattttcga agagtattag
    18121 tcaatcttat gctgtttttt ccaagattca atggcccatt tatggctacc acgtttaagg
    18181 tttttgatag cctcgtcaat agggaaccag gcaatatgat taaagttttc tagtggcttt
    18241 tgtacttctt tgaaaggagt tgcttcatag aggtaggcag gattgtagta gtaggtatca
    18301 cgatgacgag aatagaaata ttcgtcagct tgtccgtaat aggtaccaat ttctgctgtg
    18361 aaaccaagct cttcaatcaa ctcacgcttt agggcttcct gatgattttc acctgcttca
    18421 atttctccac atggtaggaa ccaagcacca tttggttctt gaacaagaac aatttgtttt
    18481 tgttcaggat tagggataac tgcatatacg ccatagcgag caatatagtc tgtattcgct
    18541 ttttttctcc gaaagttggg tttgccattg cattttcctc attatctagt atcgttatta
    18601 ttatagt
//
LOCUS       DAVLSL010000028        18573 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLSL010000028 DAVLSL010000000
VERSION     DAVLSL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.028"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(14..667)
                     /gene="phoU"
                     /locus_tag="VXN30_000751"
     CDS             complement(14..667)
                     /gene="phoU"
                     /locus_tag="VXN30_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1357412.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(679..1437)
                     /gene="pstB"
                     /locus_tag="VXN30_000752"
     CDS             complement(679..1437)
                     /gene="pstB"
                     /locus_tag="VXN30_000752"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1357413.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(1450..2253)
                     /gene="pstB"
                     /locus_tag="VXN30_000753"
     CDS             complement(1450..2253)
                     /gene="pstB"
                     /locus_tag="VXN30_000753"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1357414.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2264..3148)
                     /gene="pstA"
                     /locus_tag="VXN30_000754"
     CDS             complement(2264..3148)
                     /gene="pstA"
                     /locus_tag="VXN30_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1357415.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3138..4055)
                     /gene="pstC"
                     /locus_tag="VXN30_000755"
     CDS             complement(3138..4055)
                     /gene="pstC"
                     /locus_tag="VXN30_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1357416.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4055..4933)
                     /gene="pstS"
                     /locus_tag="VXN30_000756"
     CDS             complement(4055..4933)
                     /gene="pstS"
                     /locus_tag="VXN30_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1357417.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5507..6811)
                     /locus_tag="VXN30_000757"
     CDS             complement(5507..6811)
                     /locus_tag="VXN30_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1357418.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6812..7585)
                     /locus_tag="VXN30_000758"
     CDS             complement(6812..7585)
                     /locus_tag="VXN30_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1357419.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7575..7853)
                     /locus_tag="VXN30_000759"
     CDS             complement(7575..7853)
                     /locus_tag="VXN30_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1357420.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7853..8254)
                     /locus_tag="VXN30_000760"
     CDS             complement(7853..8254)
                     /locus_tag="VXN30_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1357421.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(8464..9081)
                     /locus_tag="VXN30_000761"
     CDS             complement(8464..9081)
                     /locus_tag="VXN30_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1357422.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(9071..9844)
                     /locus_tag="VXN30_000762"
     CDS             complement(9071..9844)
                     /locus_tag="VXN30_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1357423.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(9855..10592)
                     /locus_tag="VXN30_000763"
     CDS             complement(9855..10592)
                     /locus_tag="VXN30_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1357424.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(10597..11727)
                     /locus_tag="VXN30_000764"
     CDS             complement(10597..11727)
                     /locus_tag="VXN30_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1357425.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSTVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(11811..12200)
                     /locus_tag="VXN30_000765"
     CDS             complement(11811..12200)
                     /locus_tag="VXN30_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1357426.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(12216..12599)
                     /locus_tag="VXN30_000766"
     CDS             complement(12216..12599)
                     /locus_tag="VXN30_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1357427.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(12600..13388)
                     /locus_tag="VXN30_000767"
     CDS             complement(12600..13388)
                     /locus_tag="VXN30_000767"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1357428.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGAFVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(13381..14316)
                     /locus_tag="VXN30_000768"
     CDS             complement(13381..14316)
                     /locus_tag="VXN30_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1357429.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(14446..14622)
                     /locus_tag="VXN30_000769"
     CDS             complement(14446..14622)
                     /locus_tag="VXN30_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1357430.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(14762..15469)
                     /locus_tag="VXN30_000770"
     CDS             complement(14762..15469)
                     /locus_tag="VXN30_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1357431.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            15620..16648
                     /gene="queA"
                     /locus_tag="VXN30_000771"
     CDS             15620..16648
                     /gene="queA"
                     /locus_tag="VXN30_000771"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1357432.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(17309..17698)
                     /locus_tag="VXN30_000772"
     CDS             complement(17309..17698)
                     /locus_tag="VXN30_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1357433.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(17752..18435)
                     /locus_tag="VXN30_000773"
     CDS             complement(17752..18435)
                     /locus_tag="VXN30_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1357434.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGPTLDKSQEVGEIG
                     KLLLLQSIL"
BASE COUNT         5481 a         4297 c         3407 g         5388 t
ORIGIN      
        1 gaattaaact attttatagt tcgacaatct tacctgtttc aaagtagaca acccattcac
       61 agatattttt agcatagtca ccgatacgct ccaagtagga aataacttgg aaataatcac
      121 gacccgtaac aatggcttct ggatttttct taatctcttc agtcgcaagg tcacggatag
      181 tttcaaaata gtggttaatt tgctcatcca tggaggccac ccggtatgcg tcgtcaacag
      241 aaccattaag ataaagatca agtgctgctt caacaacgct tttaacttca cgtcccattt
      301 ttttaatttc ttcctctaca gctggaatgc gctcttcccc cttcatacgg atggttgcct
      361 gggcaatggc tacagcgtga tcccccatac gctccacatc tgatacagcc ttaaggacag
      421 tcaagactgt acgcaaatct tgagagactg gttgttggag tgcgatcatt tcaaatgatt
      481 tcttttccag tttcacttcg tattcattta cttctgcatc atcttcgatg acctcttttg
      541 ccaggtcacg gtcatgcgtg acaaaagcac gtaccgtacg attgatttgt gagagcactt
      601 cttgtcccat agcgtagaac tggttatgta atttctctaa atcttcttca aattgagatc
      661 gtaacatctt tcatctcctt atccaaattt tcctgtaata tagtcttccg tttccttgtg
      721 ttggggatca aggaacatct gcttggtatc attaaattca atcaaatctc catctaggaa
      781 aaatcctgtc ttatcagaga tacgtgaagc ttgctgcatg gaacgggtta ccagaagcat
      841 ggtgtacttg tcttttagac catacaaggt ttcctcaatt ttaccagctg aaatcggatc
      901 caaagccgaa gttggctcat ccaagaggat gattttagga ctagttgcca agacacgggc
      961 cacgcagaca cgctgctgtt gaccacctga caatccaata gctgaatcat atagacgatc
     1021 cttgacctca tcccagatag aggcaccttg caaggctttt tctacggctt catccagaac
     1081 ctgcttatcc ttaattccat tgatacgaag cccgtagaca acattctcat agatagtcat
     1141 agggaaagga ttaggttgtt ggaaaaccat tccgatttcc ttacgtaatt caaccgtatc
     1201 tgtacgcgga ctgtagatgt tgtggccatt gtacaccacg gatccagttg tggtcacctc
     1261 tggattgaga tctcccatgc ggttgagaga cttgaggagg gttgacttcc ctgatccaga
     1321 tggaccaatc aaggctgtaa tttccttagg ttggaaagat agggaaacac tattcaaagc
     1381 cttcttttta ttataataaa cggacaggtc tgatacctgt aaaatcgcat ctgtcatacg
     1441 gtttcctttc taaccaaagt gaccagatac atagtcattg gtggactgta gcttggcatt
     1501 ttggaaaata gttgcagtct tgtcatactc aatcaaatca cccaagtaaa agaagcctgt
     1561 gtagtcactt gcacgagcag cctgctgcat attatgcgtt acaatgatga tggtaaagtt
     1621 tttcttgagc tcaaacatgg tctcttctag ttgcatggtc gcaatcggat ccaaggctga
     1681 ggctggctca tccattaaga ggatatctgg cttaacagag atggcacgag cgatacagag
     1741 acgttgttgc tgaccacctg ataaggtcaa ggctgacttg tggagatcgt ctttaacctg
     1801 atcccagagg gcagcctgac gaagggaggt ttctacgatt tcatctagga cttgcttatc
     1861 cttaactcca gcacgttcat gcgcaaaggt aatattacgg taaattgact tagcaaatgg
     1921 attggggcgt tgaaaaacca ttccaatgtg tttacgcatt tcataaacgt tgatttctgg
     1981 acggttgaca tcaattccac gatagagaat ctgcccagtt actttagcaa tatcaatggt
     2041 atcattcatg cgattgagac tgcgtaagta ggtagatttc cccgatcccg acggaccaat
     2101 caaagctgta attttatttc tttcaaattg catatcaatc cccttaatgg attcattttt
     2161 accatagtaa acatggacat ccttagtaga aagggctact ttttcttcag gaaaggtaag
     2221 gatatgcttc tcatcccagt tatatgttga catggcttct cctttaggca gcggttaatt
     2281 tcttgtgtag atagcttccg aacttacgag ctccaaagtt aaaaatcagg ataaagatca
     2341 ggagcacagc ggcagaacct gctgatacaa tggttccatc tggaatagtg ccttcactat
     2401 tgactttcca gatatggaca gccaaggttt ctgcttgacg gaagatagag atggggctag
     2461 tcacactgag gatattccag ttagaccagt caagagctgg cgccgattgc cctgctgtat
     2521 agatcagagc tgcagcttcg ccaaagatac gaccagatgc caagacgaca cccgttacaa
     2581 tacctggaag cgcttctgga ataacaacat gaaccactgt ctcccagcga gaaatcccaa
     2641 gagccagacc agcctcacgt tgggtatggt gaacgtgttt caaactatcc tctacattac
     2701 gcgtcatctg aggcaagtta aagactgtca aggccaaggc acctgaaatg attgaaaacc
     2761 catactcaaa ctggactaca aagatcaagt aaccaaagag acccaccacc actgatggta
     2821 aagaggacaa gatttcaata caagtccgca caaagttggt aacaggacct tttttagcat
     2881 attcagccaa gtaaatccca gctcccatag aaagaggtac agaaataatc aaggtaatga
     2941 ccaataggaa aaaggaattg taaagctgaa tgccaatccc accacctgct tgaaaagcag
     3001 aagaccttcc agtcaagaaa gaccaagaga tatggggcaa gccccgaacc aagatataga
     3061 gaattaagga agccaagatt gtcacaatga tgctagcaat cgtatagagg acagctgttg
     3121 caagtttatc taatttctta gcgcgcataa tttttctttc ctctttcttt cgtaatcaat
     3181 ttaatcacac tgttaaaaac taaactcatc aagagcagta ccaaggccag tgaccagaga
     3241 acattattat ttacagttcc catgacagtg ttcccaattc ccatagttaa tatagaagtt
     3301 aaagttgcag ctggtgtggt caaggaagtt gggataacag ctgagtttcc gacaaccatc
     3361 tggatagcta aagcctcacc aaaggcacgc gccatcccaa agaccactgc agtgaaaata
     3421 ccagaacggg ccgccttcaa gatcacacgc cagatagtct gccagcgagt ggctcccata
     3481 gcgaaactgg cctcacgata ataacgagga accgcacgca agctatccgt tgtcataaag
     3541 gttacggtcg gcaaaatcat gacaaagagg acggaaatcc ctgacaaaat cccaaaacca
     3601 gtcccaccaa agacactgcg aacaaaggga acgacgactt gcaagccaat aaatccgtac
     3661 actactgaag gaatcccaac caggagttca atagctggtt gcaaaatctt cgcccctttt
     3721 ggtgatactt cggtcataaa aactgctgca ccaatagcaa agggtgttgc gataagggct
     3781 gagagaatgg taacgataaa ggaacccaaa atcataggaa gggcaccaaa ttctttacta
     3841 gaaggattcc aagttcctcc caaaagaaag tcaaagatat tcacaccatt gacaaagaag
     3901 gtcgacaagc ctttttgcgc tacgaaaacc aaaatcatgg ccacaaggat gactatcaaa
     3961 gaaagacagg caaaggtcaa accttttcct aatttctcca gacgagaatt ctttgatgga
     4021 agcaacattt tcttagctaa ttcttcttga ttcattattg tctcccttcc aacactgtca
     4081 cagttccggc agcatctttt tcaaccttca tttccttaat cggaatatac ttcaatcctt
     4141 tgacaatccc ttcttgggtc tcatccgaga gaacaaaatt gagaaattct gcagccaact
     4201 cattgggctg ccccaatgta tacatatgct cataagacca caagggccaa ttattgctac
     4261 ttatattttc tggacttaag tcatagccat tcaacttcat gcttttgacc gaatcatcta
     4321 tataggtaag agataaataa gagatagctc ctggactttt tgatacgatt gattttaccg
     4381 ctccatttga atcctgctcc tgactttgca tggcagactg accttccata atgacagtat
     4441 caaaggtagc acgagaacca gagccggctg cccgattgat aacagagatg ggtaagtcct
     4501 taccaccaac ctctttccaa ttggttacct cacctatgaa gatttgacga agttgctctg
     4561 tcgttaggtt atcaacatca acctccttat tgacaatcag agccaagcca gctaccgcga
     4621 ccttgtggtc aacaagagca gaagcatcaa ttccgtcttt ttcctcagca aatacatctg
     4681 agtttcctat atcaactgcc ccagactgaa cctgggacaa gcctgtacca gaacctcccc
     4741 cttggacatt gaccgttttt ccaacatgga tcgtgccaaa ttcatctgcc gctacttcaa
     4801 ccaagggttg caaggcagtt gagccaacag ccgttatgga ttctccacga tcaatccagc
     4861 tagcacagcc tactaaacaa gccgtcagcc aaaaagcgat aagagacaga gcaagctttt
     4921 ttcttttttt cactgttttt ctcctcgaaa ataattatga atactgtgaa ttttttaagt
     4981 agttctttat gagttgacgc atgaattctt accaaatttc tgcgcaattg attatttata
     5041 taatataggc tatattactc tttcctaacc tccttttttc atatgtggat aaaatctctt
     5101 gtctatccct tcccccattg tcacccatta tagtcatttc gtgtctcttt ttcccctttt
     5161 taatgcaagg gaaattactc tccttagatg ataatccaaa agctagaaag gtatctcaaa
     5221 cctctctact ctcccagact agtttacaac taaaaggaaa agattctatt ttatgagaaa
     5281 tctagtttac aagcggtaag aacgctaata actaaacttc ttgtactctt tgaaaatctc
     5341 ttcaaaccag tgttttgagc tatctatggc tagcttccta gtttgctctt tgattttcat
     5401 tgagtagtaa aactacatgt aatggcaatc aagatatcaa gaatcatcct actaaaaaaa
     5461 tccatacttt cactataaca tagaataaga tatttgacta gcattttcat ttgaatctga
     5521 ggccttttgg aaaataattt ttcaaaacat ttccagtaac ctttgcaaag cccaagccat
     5581 tgcctttaac caaaacttgg taccaaccat ttggcagact ttctgccagc tgaacgattt
     5641 ctccagccgc atacttgaca aacgcttctt ggccaatttc aaccgactgt tcgacctgac
     5701 tcggtttcaa ggctaaacca agagcgaaac tgggctcaaa gcgtttcttc ttaaaagtgc
     5761 ccagatgcag tccattgcga gcaatcttga gcttccctaa atctggcaaa agttctggca
     5821 agagataaag ctggtctcca aaagtctgca agatacccgg tagattgacc ttcaaatggt
     5881 tttgggcaaa ttcctgccac aaggcaactt gttcacggct gaggttactc ttacttgcct
     5941 taaatttagg agctggattg ttacccttaa actgtagatg ggcaacaaac tgaccctctc
     6001 ccttaaactg atgagggtac atccgagccg tttctggcag gtcaatacca gctaccattc
     6061 cattgatatg ctctactggc aacaagtcaa aatcatactc ttccagtaac caattgacaa
     6121 tctcttcgtt ttcctcgggt gcccaggtac aggtcgaata aaccagacga ccaccttcag
     6181 ctaacatggt cactgcatcc tccagaattt ctctttgcaa gctagcacat tgactcggat
     6241 aatctaagct ccaatagtcc atagcatcag gttgcttacg aaacattcct tcaccagagc
     6301 aaggggcatc aagaacgatt aagtcaaaat agcctttaaa gaccttgacc aagcggtcgg
     6361 cagattcatt ggtcaccacg acatttgtcg ctccaaaacg ctccatgttt tcaaccaaaa
     6421 tcttagcccg tttgcttgaa atttcattgg aaacaagtag cccctcccct gctagatagg
     6481 ctgccagttg agttgatttg ccccccggtg cagcagccaa gtccaagacc ttcataccag
     6541 gactgggttg agctacttga gccaccattt gagcagcagg ttcttgcgaa taaactaaac
     6601 ctgtagcatg ctcaggcgat ttccctgaaa ccttcccata gtggccccaa ggggtttgag
     6661 taatggcatc agaaaaggaa agttgctctt cttttaaggg attgacccga aaggccgaaa
     6721 ccgcttcctc ctcaaaagag gcaagaaaat ctcttgcctc atctcctagt atctctttat
     6781 atttttcaac aaatccttct ggaaattgca tttaagttct tttcctttcg taaatatagg
     6841 actgaatttc ctcctgcatc tcaagaggca ccatcatgac cggctgtctg gtttgaaaat
     6901 caggagcttc accaaaaagg gtcacaaccc gatagcccag actttcccct aaaatactag
     6961 ctgcggcata atcccatggt tgcagataag tgagataggt caacaaacgc cctgacaaaa
     7021 tcttggcaaa actaatggcc gcacttccat agacacgaac accaagaacc gctcggctca
     7081 aatcagccag cccccattca ttggtttcca gcataccact attccctgca atgagaaaat
     7141 ctccaagtgg tttagtttta aaaggagcta ggggcctatc atttagacaa actggaaatt
     7201 ccccaccacc gtggtaacaa tcccctttga ccacatcata aatcagacca aactgtccct
     7261 gaccattttc aaaataagcc atcataacag caaaatcttc ctgctgggct acaaaattat
     7321 tggtaccatc aatgggatca atgacccaaa ccttgccctc ttgaaccgag gctcgcagac
     7381 aaccttcttc agcacaaatc ttatcctcag gataacggga caaaatctca ccaaccaaga
     7441 gttcctgaac ttctttgtcc agtctggtca ccaaatctgt tggagaggac ttggtttcaa
     7501 cacgcaagtc ttcctgcata tggtcaagaa tgtactgacc tgctttctta acaagctctt
     7561 tagcaaattc aaatttactt tccaagagaa atctttcctt cccctttttc tttggcagcc
     7621 tgaactgctc ggtaaagtga atagccacta accgtttcaa attctcgtcc caaacgtttc
     7681 tcttcgctct tgcttggcac gaccgctttg aatcccttat aggagtccag taacttttta
     7741 gcctctactt tgccttcata ggcagcttca acatcattaa aaaaagaaag cactgaagca
     7801 agttcttcag tgctccacga caaatctagt gggtaactat actgtttgtt cattaactaa
     7861 taccagctct cattcttgct tcttttagtt cttgcttacg ataactacga gggagaaaag
     7921 cacgaatctc atcttcatta aaaccgattt gcatacgctt ggcatcaata ataattggac
     7981 gacgcaaaag actaggatac tgctcaatca aatgaagcaa ttccgatacc gaaatactct
     8041 ctacatcaat attcaatttt tgaaaaattt ttgaacgagt tgaaatgatg tcatcagtac
     8101 cattttcggt caaggaaagg atgtgttgca attcttttct tgttaaagga ctggtcataa
     8161 tattgtgttc cacaaaggga accttatgtt tttctaacca ggccttagcc ttacgacatg
     8221 atgtacagct cggtgataga aatagtgtaa tcatgctttt ctcttcttat ctatactttg
     8281 ctacttctat tatacaaaaa aataaggcgc ttgactaggg atttttagaa aaaaagccta
     8341 ttttttcaag aaaaataggc tttttgcgaa cgattgacac aattggattt ggttaattca
     8401 ctcttaacga tggttttaaa cgatatatat ttttatatat gtaaattaaa aacatctttc
     8461 ctttcacttc ctacgacttt tcagatacag atagccaaag aagttttcat agagggcaaa
     8521 aaagaggagg aaggcatgaa gaaagaaggt ctctggcaaa atcataataa caggatcctt
     8581 ggctggatca aaaagccagg tatcatctcc cacaaagaga atttgatgga aaagagtaaa
     8641 gaattggtca aaaccaatca aaactccccc aagtccaatc atcacaggta agactactag
     8701 agccaggaga ctttttcgat aaagagacaa aaagtccttt ttcacaatcc tattgacaaa
     8761 gacatagaaa cttggcagtg tcactagagc tactagctga accaaatgaa agagattctt
     8821 gaccactgcg aaatggtgca gaccagctgc tgacgaacga aaatcaggca tctgtaagac
     8881 ctgactaaaa ggattggtca gataattcat caagatatga aaattgtatt gaatggtttc
     8941 tggttttaga tagactcgat tcgttaagtt tagccactga atctccatag gatagaaaat
     9001 ccaagccaga taaatggtca gaaggattga tagggagagg agaaagagca tagagcccca
     9061 aaaggtcaat ttagttttca tcaaaatccc actccgcaag gctagaaacc acatgtgtcg
     9121 gtgcgattgg caggccagct acttcttctg ccttagtaaa acctgtcgtc accaagagcg
     9181 ttggaatgcc attgtcaatc ccagcccgga tatcagtcaa ataattgtcc ccaaccatga
     9241 ttaactcttc acgttccaaa cctaagtgct caaccgcctt gtccataatg atggcatttg
     9301 gttttccgat ataaaccggc ttcactcgtg tcgctacttc aagcagcgta atcagtgagc
     9361 cagcacctgg caaaagacca cgttccgtcg ggatgttgag gtcaggattg gttccgataa
     9421 aatgggcacc cttttgaata gcaagagttg ctgtggcaaa tttttcatag tcgacttgcc
     9481 aatccagacc aactaccacg taggcagggt tttccttgtc ttccacataa ccagccgcct
     9541 tgatggcttc cttgagtcct gcttctccga cgacatagac agtcttttca agccccaaat
     9601 cattcatata gtcgatggtt gccaaagtcg ctgtgtagac agtcgataga ggcgtatcga
     9661 tattaaaatt ctgagccaac atctccttaa cactctctgg agtgcgggtt gtattgttgg
     9721 ttacaaagag atagggaatg tcccgctttt gcaattcatg aacaaaagtc tctccagcag
     9781 ggattcggtc tttcccctta taaatggttc cgtctaaatc aattaaatag cctttatatt
     9841 tcatctattt ctccctaaac cttttttatt tcttgccaag taatgattgc ttgggcattg
     9901 ataaccccat cacttgtaat ttcatgcttg ctttccagtc cagtccgttc aacagccgat
     9961 gtaatcaccc cacctggtcg aacttccttg acatacttga ggttgatttt cttgggaata
    10021 tagtgggtca aaaaatccgc tcccatgacc tcaaaaatcc agtccaagta tttactgtta
    10081 ttgacatgac cattcatatc caagtcgtaa aaacgaacat ggtaatcctt gctgatcggc
    10141 tcttccaagg attcatactt cggtccacgg ataagttttt tatcaaaatc agactggtaa
    10201 ggagccacaa tctcaggttc aacagcatgg acttttcgac tgtcgcggtc catgagaaca
    10261 aaggtcgcca tcatatggat gagctcctgc cccgcttcat tataaatagt aaagcgacgg
    10321 tagcaaaaaa gtcgattgta gctcaaggct tccgtttcga tggtaatttc ttccgcaaaa
    10381 cgaggcaaac gaaccacctc aatatcatat tctgcgataa tccagaccag attatagtct
    10441 tccaaaatgg ccttatcact aactcccagt tcaatcgatt gcatccctga aacttgcagt
    10501 gacagcaaaa tcacatctgg aagtttgata tgaccgttca tatcagccat atcaaaagga
    10561 attttcattt tcatttgata agttaagccc atgatcctac tccaaaataa atcgttctgc
    10621 tacagtatct cccaaaaaga gacctctctt tgtcatgcga acgtggtcac cctcaatctg
    10681 catgaggcct tgttgaacca aatctctgac aatttctcca taaagtccag caaaagactg
    10741 tccaaatttt tcctcaaatc gcgccatgga aaccccggat ttcttgcgga gtcccaagaa
    10801 catttcttct tccatttgct ccttttgact caggtgatct tctgtaatac aagcattgcc
    10861 ttcctcaacc gcactaagat aatgacgaat gggaccatga tttttatagc gtactccatt
    10921 gacataacca gatgcccctg caccaatacc atagtattca gcattgtccc agtacatgag
    10981 attatgacga ctttcaaagc cgggtttgga gaaattagaa atctcataat gctcaaaacc
    11041 cgctcgctcc agctctgcaa tgatgtactc aaacatctcc gcttctagtt cctccttagg
    11101 cagaggcaat ttcccacgtc gcatccggtt cataaagacc gtatggtttt ctaaaatcaa
    11161 actatacaaa ctcatgtggg gaatatccag tccaatggct ttagccacat tttcctttac
    11221 ttgctccatg gtctgaccag gcagagcata aatcaaatca atggagatgt tgtcaaaacc
    11281 agccagtttc aggcgatcga tattttcata aatatccttc tccaaatgac tgcgcccaat
    11341 ctttttcaac atcttatcat caaaggtctg gacacctagc gaaacacgat tgacagtcga
    11401 atttttcaaa acagctatct tatccgcatc caaatcgcct ggattggctt caatggtcaa
    11461 ctcttccaag acagacaagt ccaaattttt agtcaagcca ttcagtaaca cctccagttg
    11521 cggagccgac agggctgtcg gtgttccgcc accgatataa agggttgaca acttttcaat
    11581 atcataagaa cgaaactctt ccagcagatg ctctaaatag ctgtcgactg gctgattttt
    11641 gatgaagacc tttgaaaaat cacaataata acaaatctgg gtacaaaatg ggatgtgcac
    11701 ataggctgac gttggttttt tctgcatagt aattattata ccacaaagac tagattccag
    11761 ataaaaatca ccatccccag atacatagtc cgtccggaga tggtaatggt ttattcttct
    11821 gttatatcaa tcacaatctc ttctgagtca tcaagagctt cggctttttc ttgccattgt
    11881 tccttgagat tatttaattg attttttgat gcttctgtcg cttgaaaagc ataggattta
    11941 gcttgagcaa gtatactgtc cacagtgatt tcacctgact caacctgttc ttttgttttc
    12001 agaacaaaat ctgtagcctg ctccttaact tctgtcagtt tttcacagac ttgctccttg
    12061 gcatactccg gatcttctct caaatcatct agaaaatctt gagcctgact gcaaacttgt
    12121 ttgcccttat cacttgttaa aaacaaggca agagctgcac ctgaaaccgt tcctaaaagg
    12181 attgaggata atttacccat aaggattctc cttttttatt ttttgaaaaa tttacttgca
    12241 agacgaagag ctgacagact tgcaccagtc ttgagtgttt ttgaaccagc tgatgaagct
    12301 ttcttgctca agacacgcgc atggtcattg aggtctgaaa cagatagaga taaatctgca
    12361 acagcactga agagtggatc aatcgtagcc accttgacat tgatatcatc tgccaagaca
    12421 ttgaccttag ccaacaactc attggtgtga tgcaaggtca catccacatc tgaagtcaag
    12481 gttttaatcg tcttttctgt ttcatcgatg acacgaccaa gcttttgtac agtaatgatc
    12541 agatagacca aaaagacaat caaagctagg gcaacaagaa tatatgcaac ttctaacatt
    12601 tagttttcct cctctgtaat atagtaaggg gccttctttc gattttgata aataacgatc
    12661 attataccga gaccgataag gacaactgac agccattggg acactcgaag gccgaagaac
    12721 atgagactat ctgttcgcat accttcgatg accatacgac cgaaaccata ccaaatcaag
    12781 taaaaggccg tgatatgacc tcgtctgaga ctcttccatt tccgtctaaa aatcagaatc
    12841 aaggcaaagc caagcagatt ccatagagac tcataaagga aagtcggttg acggtagctc
    12901 ccctcaatat acatctggtc acggataaag ccaggtagat aatccagatt atccactgtt
    12961 gcaccataag cttcttggtt aaagaaatta ccccaacgcc ccaaactttg agcaatcata
    13021 acgctaggcg ccgcaatatc tagaaaatcc caagtattga tgagtttacg gtcagcaaag
    13081 atatagagca caaaagcccc agttatcaaa ccaccgtaaa tggccaaacc accattccaa
    13141 atggcaaaaa tctctcctaa attctgacta tagtaatcaa atcggaaaat aacatagtag
    13201 agacgagctc ctaaaatagc caagggaaag gctactaaga taaaatctaa aatatcgtct
    13261 ggtatgatct tctttctagg tgcttctttc atggtcaaat aaaccgcaag aatcaagcct
    13321 gtcacaatac ataaggcata ccaacgaatg gctaggggtc ctagttgaat agcaattgga
    13381 tcaagcattt tgcacctcat ttcgagcgat gagacttgtc agtcgttcgt cgaacaaacg
    13441 ggtcgcatca aagcccattt ccttggcacg ataattcatg gcagctgcct caatcacaac
    13501 agagatatta cgacctgttt taactggaat acgaatacga ggaatggcta cgccagaaac
    13561 ttcaagttcc tctgcattgt ttccaagacg atcaaaggtc ttatgcgtat cgtaattttc
    13621 caaatagaca gcaagctgaa cctgtgaaga atccttgaca gcactcgcac cgtagagact
    13681 cataacatcg ataataccaa ccccacgaat ttcaatcaag tgtttcaaaa tttcagctgg
    13741 ttcaccccag agagtaatct catccttggc aaagatatcg acacggtcat cggctaccaa
    13801 acggtgacca cgtttgacaa gctcaagacc tgtctcgctc ttaccaattc cactatctcc
    13861 ctgaatcaag acgcccatcc cataaatatc catcaagaca ccgtgcacac tggtacgttc
    13921 tgccaaacga gaatccagat agctagataa ctctccagat aaacgactgg tagctgcacg
    13981 gctggttaaa atagcaatct tacattctct agcagccttt aacatctcct ctggaaccac
    14041 caaaccacgg gcaacaatga ctgctggtgt ctcaggtaga aacatttttt tcaaaacttc
    14101 ataacggctg ttggaaggca tgctgatcag ataagaccac tccttcatcc ccaaaagttg
    14161 aatccgctct ggtgtatagt agtcaaaata gcctgtcatt tcaagaccag gtcgcgtaat
    14221 atccgctata ttgatttcct tttcaagcaa ttctggttca ccatagacaa tatctagtct
    14281 aagcttttca atcacttctt ttactaaaac cgacatcatt tcctccttca aatgagttct
    14341 tctctctatt atatcaaatt gtaggaggaa gtttcagatt tttgcaggct ttggagtatt
    14401 tatttaaaag aaaaaggact agatttctct agcctttcat ttcaattaga attttttacg
    14461 tttacgagct acttctgatt tacgtttacg ttttacagaa ggtttttcat agaattcacg
    14521 tttgcgtgtt tcttgaagag taccagcttt agtaaccgca cgtttgaaac ggcgaagtgc
    14581 gtcgtcaaga gattcattct tacgtactac tgttttagac atttttttca ctcccttcaa
    14641 gttcaatatc tatttcattc tacacgtaaa aggaataatt gtcaagcaaa aatgcggttt
    14701 aattttgatt ttttcttcca tttatacaag agttgaaagc gttcaataga acattgcttt
    14761 tttatttttc gagtaagcta agcgcttcag catctgcgat gatggttaca tcagggtgat
    14821 tttgaaggct acttgcaggt aggttctcag tcactgggcc agatactgtt ccggcaatgg
    14881 cttctgcttt cgactcaccg taagcaaaaa gaataataga cttggcatcc aaaatgtttt
    14941 taatccccat tgaaatagct tgggttggga cgtcttcaat cttggcaaag aagcgtgcat
    15001 tggcttcgat agtagactgg tcaagttcta ctagatgcgt ttgactgtca aatggagtac
    15061 caggctcatt aaagccgata tgcccattgc gaccgattcc caagatttgg aggtcaactg
    15121 gatggtcagc caaaatttgg ttgtagcgtt caacttcagc ttcagcattg tccttaacac
    15181 cacgaggcaa gaaactttct ttaaatggtt tttggttgaa caagttttct tgcatgaagt
    15241 aacgataaga ctgtggattg tccccatcaa ggcccacata ctcatcaagg ttgacactgg
    15301 ttagatttga aaaatcaagg tcactctcaa caatttcctt gtaaaattca agtgggctac
    15361 ttcctgtcgc aagtcctaga gtttgagctc cattagccaa tttttccttc aaaatctcaa
    15421 aagcaacttt tccaccttga acttggtttt caactttaat aactttcatc ttttcatcct
    15481 ccatttctgt ctatatttat tatatggtat agaccaattt ttgtcaagtg aaaacgattt
    15541 aatttctaaa aaaagagcgt ccaaacttga aacgtgttat aatggatagg attagaactt
    15601 agaaagaatg aacaaatata tgaatacagc tgattttgat ttccacttgc cagaggaatt
    15661 gattgcccaa acgccccttg aaaaacgtga tgcctccaaa ctcctcatcg tcaaccgtga
    15721 gacaggagaa atgcaagata aacatttcca ctctattatt gatatgctgg aacctggtga
    15781 tgcccttgtc atgaacgaca cccgagttct ccctgcccgc ctctatggtc aaaaagtgga
    15841 gacaggaggt catgtggaac ttctcctcct taagaacact agtggagacg agtgggaagt
    15901 tctggctaaa cctgccaaac gcctcaaggt cggtactcgt atcagctttg gtgatggccg
    15961 cctcagcgct gtcgttacag aagaattgac ccacggggga cgcattgtcc gctttgaata
    16021 ccaaggaatt ttcctagaag tcttggaaag tctgggagaa atgcctctgc caccttatat
    16081 ccacgaaaaa ttagatgacc gtgaacgtta tcaaaccgtc tacgccaagg aaagcggctc
    16141 tgctgcagca ccgactgctg gtcttcactt caccaaagaa ctgctggcag aaatccaagc
    16201 taagggtgtt catctagtct atctgactct ccatgtcgga ctcggaacct ttagacctgt
    16261 ttctgtggat aatctggacg aacacgaaat gcactcagag ttctatcaac tttctgagga
    16321 agctgctgcc acccttcgct ctgtcaaaaa aaatggtggt cgtgtcatcg ctgtcggaac
    16381 cacttctatc cgcaccttgg aaactattgg ttccaagttt gatgggcaaa tccaagcaga
    16441 ttctggttgg accaatatct ttatcaaacc tgggtatgag tggaaggtcg tggatgcctt
    16501 ctcaaccaac ttccacctgc caaaatcaac tctggtcatg ttggtttctg cctttgcagg
    16561 ccgtgaatta gtcttagatg cctaccacca ttccatccaa gaacactacc gcttcttcag
    16621 ttttggtgac gccatgttta tttattgaga aagaatttct ctaaatcttc taataccaat
    16681 aaatcgctaa gatattattt caaagaacat ctacaattga aactctagct agctgtagaa
    16741 gacgcctagt acattgaaat taaaatgctt ccccctagct tcgaaaatat tgccatagat
    16801 tgcgttgact caccaaattg attcatctat attttatttc agcttcctat attttcttcg
    16861 ctgtttgtaa atcaaaatgc aagacacatg agtagcacca tatttgttac tcttatctgt
    16921 cctctcaaga gactattatg agttatttca gaatcattca ctactttgac cctgactctc
    16981 cttagtctca aaatcaaaga cttatactct tcaaaaatct cttcaaaccg cgtcaacgtc
    17041 accttggatt atatatgtga ctgacttcgt cagttctatc tacaacctca aagcagtact
    17101 ttgagcaacc tgcgactagt tttctagttt gctctttgat tttcattgag tattaaacaa
    17161 aaagtgaaca aatctgaatt ctaatgtaca gaagactagg cttgttcact tttttatagt
    17221 cgctataaga tgaccttatc tatagctttt tatatataat tatatattca gacatactat
    17281 tatcaatttt gtcgcaggga ggaatctgtt aacgcaccca ttcaccatta tcattgactc
    17341 tatagccatc tatacttgta ttgaccgcta actcacccga tgcatttaca taataccatt
    17401 taccaccaac ttggaaccat tgattgactt tcatagaacc gttgctgttg aggtagtacc
    17461 atgaaccatt aacttgtacc caacctgttg ccatggaacc atcagtatta taaaaatacc
    17521 acataccatt ttcttgtttc cagtctgttg ttggagcaac tgctttagct ggttctactg
    17581 ctacatctgt tccttggtta gatgtaacag atacaggata cgaaggaata gatgattgct
    17641 caggaacaac aactttttca ggttctctcg tccctctcct tatacgtctt tttaccatct
    17701 ctttagtaat ttgacgagaa gtagtttctt caattgttcc atcacgttca tctacagtat
    17761 agattgtagt aagagtaatt taccaatttc tcctacttct tgacttttat caagagttgg
    17821 gccatcgaga tattctgttt cgattggaat ttcttggaca agaacttggg gcttggttcc
    17881 ttttttaaca actcttgttt gagagtcttc tttttgactt aaagtactct cagttacttg
    17941 tccactcttt ccatctacat cataagttat cgttgtaact gttttcccat tctttcctag
    18001 agtaatctct tgctcctgtc ctgcagaaag gtcattgtct gcttcatatt tagtagcaaa
    18061 tggaacaaga acttcttcaa ccttgctttt agctggaact ttgataactg tatccgtggc
    18121 ttcttttcta tcaacagtaa ccttttcggt aacatgacca gtctctggat taacatcgta
    18181 ggtccttgtc gtagttacat agccatcctc tccatcaatt gtaacaggat tttcactacg
    18241 gtcttttgtt tcatcttttt cataacgaat tcgcgtactt gaaattttct tggttactac
    18301 cttaggttta gtcgctactt ttacaataat atccccattg tcagcgtcat catactctat
    18361 tccctcttct ttatctctag tatcatctct gacatattga atcccatcag cagcatgaac
    18421 aaaacttgta ttcagattcc tcctaaaaat aaagttagcc cgattaccgc agaaccaaaa
    18481 atctttccga gtttacgtat tgcatagcgc ttattagtat tagattttgc cattacatcc
    18541 tacttctagt atagcatctt ttctatcaaa cgt
//
LOCUS       DAVLSL010000029        18396 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLSL010000029 DAVLSL010000000
VERSION     DAVLSL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.029"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            516..1379
                     /locus_tag="VXN30_000774"
     CDS             516..1379
                     /locus_tag="VXN30_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1357435.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTALFE
                     LRLQQYKKLID"
     gene            1665..1898
                     /locus_tag="VXN30_000775"
     CDS             1665..1898
                     /locus_tag="VXN30_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357436.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            1920..2597
                     /locus_tag="VXN30_000776"
     CDS             1920..2597
                     /locus_tag="VXN30_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1357437.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            2609..3274
                     /locus_tag="VXN30_000777"
     CDS             2609..3274
                     /locus_tag="VXN30_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1357438.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3346..4572
                     /locus_tag="VXN30_000778"
     CDS             3346..4572
                     /locus_tag="VXN30_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1357439.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(4969..5046)
                     /locus_tag="VXN30_000779"
                     /pseudo
     CDS             complement(4969..5046)
                     /locus_tag="VXN30_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            5117..5773
                     /locus_tag="VXN30_000780"
     CDS             5117..5773
                     /locus_tag="VXN30_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1357440.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            5763..6086
                     /locus_tag="VXN30_000781"
     CDS             5763..6086
                     /locus_tag="VXN30_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1357441.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            6190..7020
                     /locus_tag="VXN30_000782"
     CDS             6190..7020
                     /locus_tag="VXN30_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1357442.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            7174..8028
                     /locus_tag="VXN30_000783"
     CDS             7174..8028
                     /locus_tag="VXN30_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1357443.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            8128..9501
                     /locus_tag="VXN30_000784"
     CDS             8128..9501
                     /locus_tag="VXN30_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1357444.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            9494..10555
                     /locus_tag="VXN30_000785"
     CDS             9494..10555
                     /locus_tag="VXN30_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1357445.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMAKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            10557..11249
                     /locus_tag="VXN30_000786"
     CDS             10557..11249
                     /locus_tag="VXN30_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1357446.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(11279..11848)
                     /locus_tag="VXN30_000787"
     CDS             complement(11279..11848)
                     /locus_tag="VXN30_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1357447.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPTPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            11983..12597
                     /locus_tag="VXN30_000788"
     CDS             11983..12597
                     /locus_tag="VXN30_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1357448.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            12599..14245
                     /locus_tag="VXN30_000789"
     CDS             12599..14245
                     /locus_tag="VXN30_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1357449.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTLSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            14257..15543
                     /locus_tag="VXN30_000790"
     CDS             14257..15543
                     /locus_tag="VXN30_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1357450.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            15595..16128
                     /locus_tag="VXN30_000791"
     CDS             15595..16128
                     /locus_tag="VXN30_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1357451.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            16206..16676
                     /gene="nrdI"
                     /locus_tag="VXN30_000792"
     CDS             16206..16676
                     /gene="nrdI"
                     /locus_tag="VXN30_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1357452.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(16969..18231)
                     /locus_tag="VXN30_000793"
     CDS             complement(16969..18231)
                     /locus_tag="VXN30_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1357453.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     TEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         5330 a         3396 c         4052 g         5618 t
ORIGIN      
        1 tgaaaagaat tttgaactat ttacaaaaca aagtgatcta attgttgcaa atagatggga
       61 taggggcctt gaagcttata aagataaagt ttacacaagg gggatctgga taagagatta
      121 actattgata ttaaaaaggc aataatttat aaaatttgta caaaaaaatg ttaaattttc
      181 acattttcat agtatactgt ttagtagaaa gtaggttctt tgtaaccgct ccattataaa
      241 atcctttcac tcttccggta tactattagg aaaaacataa ctggaggata ttttgagctc
      301 acacctcatc gttccagtgg ctgttcccca agctcgtgta ctgcccaaaa aagttagaca
      361 ttctatttag aacgaggatt gagttctgta ttgtacaagg ctcggtcctt ttagagtcag
      421 cttaaggctg gctttttcaa tcacaaaagt gtcagaatgt tttgacaaat gaacacaaat
      481 aatgatataa tatgcaaaag ctaggaggtg gtaggatgat tgaaaaaatg gaactggggg
      541 aattttacaa ggaattacgt ttggcgagaa agcttaagca gacagatgtg gcttgtgagg
      601 gtctaacagc ttctcagttg tccaagtttg aactagggca gtctatgctg tctgcggata
      661 agttgattct agctatccaa ggtataaatg tgacctttga tgagtttggg cacaagctca
      721 acaactatca agaatctcca catatgcgaa tcggtcgaaa ggttgtgaat cgctttgccc
      781 atcaggatat agctgcttta gagcagctat tggaggaagt cgatcaagaa cagatggcgc
      841 agacctatcg tcgtttgaat gccatcgtga ttaaagatgc catccattca ctaaataaaa
      901 gctacccact agcagaggag gatagcgagt ttttgaccac ctatctctat gctatcgagt
      961 cttggacctg gtttgaactc tatctctttt gcaataccat gcccttcttg agcaatcaag
     1021 atctgatttt tttatcaacc tccttactcg aaaaatccaa agaatttaaa gagttagtac
     1081 acaatcgatt gtatatgaag caaggactct taaatatctt atcagaactc atggagcgaa
     1141 aacttttctc ctacatccca atcttcgaag ccgagttgga gaggatgtta cgaccgtatg
     1201 atgtttttga aaaagtatcg tggcaatttt taaagaagat gagtgtcttt cttcaaacaa
     1261 agggaagcaa tcaaaaagag attgaacgct ttatccaatc tctgcaagta ttagaaaatc
     1321 cacaattgac agcccttttt gaattgcgtt tgcagcaata caaaaagctt atcgattagt
     1381 tagaaatggc aaaataataa taatctcagc aacgattttc aataatgtta aaatttgttt
     1441 cttcatattt ctccactcct cattagtagt aactttatta taccagagaa gtaatcaaat
     1501 tcaccaaaat cgcaaatctg aaatttctat aagaaaaaat atcaaatatg cgattttcta
     1561 aaataagcca attttcgtgt tatactgtac ttgtaaagca cttgaagcaa atcctaggtc
     1621 gcagaagtgg tttacaaatg aagataattg aaggagtgta aaagatgtta aatttacaat
     1681 ttgcagaaac aatggaattg acagaagctg agttgcaaga tgttagagga ggcaacctcg
     1741 taaattctat gggaggtggt ggaagaagtg gtatatctgg ttggggcgtc ccaggaattt
     1801 accctggttg gggcaatcaa gggatgagtc ctaatcgtgg agcttttgat tggactatag
     1861 atttagcaga tggattgttt ggccgccgta gaagataggc tcttatagga gataatagga
     1921 tgaagaaaat gaaagaagtg aaatttcacc tagcaactgg cttgttgatt ttgacttatt
     1981 acctcatttt taatgtcaca tcagacttag attttatggt tgctttgtct gataatatgt
     2041 actatgtgtt ccaagtttta ttggtactta ttctgggaac tatagctaca attgcttttg
     2101 tcaaaagtga acattggaaa gagtgtggtc gtttccagtt tcgttggtct tacctgggtg
     2161 ttttccttct ttcttttttc ttattgttcg tgtgggcaaa tctaacaacc tatatctttc
     2221 ctcgtaccca gaatggaagt acagtcgtag aagtagccac caatttaaca ggaattagct
     2281 attttgttac gcgaatactt tatactagta ttatcgcacc tgtatcagaa gaggttgttt
     2341 gtcgtggtct tttgatgaca agtttatcta aagttaagag atactatctt gatgttcttg
     2401 tttccgctgc catatttggc gctatgcatg ttttgcagta tggttggata acgacagatt
     2461 tcataaagta ttttggaatg ggcttgatat tttgtatgat gtttcgctac acccgatcca
     2521 tctattgggc tatagctctg catgcttcat ggaatagttt cttactcatt gttactttat
     2581 tggtatttgg atattagaaa ggtgatatat gaaaaagata atctcacacc gctactttat
     2641 tatagttttt ctactagtta ttgctgacca gaagttcagt gttttagttt tacgcagcga
     2701 ccttgttgct gggctatctg actttgccta ttatctgtcg gatatgatgt tgaattttct
     2761 tgtggtttta tttgctctta ttgctatgat ttggtcgggg aaatggcaga aaatcaatag
     2821 tagaaagttt aaaggatcct atcttttcta ttccttccta gctctgcttg cttttgttgc
     2881 ttggaatttt gtcacctttt ttcttttccc acctactcga aatgaaattt cttatcaaca
     2941 tgctgctcct acttttacag gagctacggc atttttgatg tatttttttt atcctgtgat
     3001 tgcaggtccc atttttgaag acatgattta tcgtggatta gtgatgactg ctctggaaaa
     3061 aggaaagaaa tgggggctag atgtacttgg ttccgctgtt ttatttgggg tctcgcacat
     3121 tagtaatcac ggttgggtct tgacagactt tgtcttctat atgggtggag gtcttatatt
     3181 tgcagtctta tttagaatga caaagtcaat ttattggcct attggactgc atatagtcta
     3241 caatggcatt ggtcagcttt tgatgttact gtaattttgg ttgttagtag tatcgcggtc
     3301 tatccttggg gaaagattcc catttgaact taagaaggag ttgtgatgaa agtatttctt
     3361 caaaatagag attttaggca attaactatc aaccagtgga tttcaacgct tggggatacg
     3421 attttttatc tggcctttct gaattatgtg gcagatgcgt cttttgcacc tttggcgatt
     3481 ttactcatta cgatttcaga aacccttccc caaattttac aaatctttct gggagttttg
     3541 gcagattttc aacatcatcg tgtcttgaaa tacacagtca ttagttttgc aaaatttttg
     3601 ctttactcta tcgtttcttt atcactttca gggcagtctt tctccttgtt gctagtagca
     3661 tttatttgtc tgcttaacct cttgtctgac acattgagtt acttttcagg agccatgctc
     3721 actccgattt tcattagaat tattgggcaa gaccatctgg cagaagctat tggatttaaa
     3781 cagtcaactg ttagtttagt gaaaacaatc agtaatattc taggaggagt cttgctaggg
     3841 attctctcca tccagttcat ttccttgctg aacgctctta cctttttaat cgcattttta
     3901 ggtatcctct tcataaaaac tgacctcttg aaagtagaaa aaacgattaa ctatcaagaa
     3961 ggactctctg taaaatcctt ttgccagcat ttgctccaat catcgaaatt gatatggaat
     4021 atgaataagg tgctcttggt tttgtttatt atctctacta gtcaagcagt gataaatgtt
     4081 acagttcctg tctctactct atttttgagg aatcaaccct ttttgaattt acaaacaggt
     4141 caatctcttg ccttgctatc cacttttgaa ctgtcagccc tgattgtcgg aagccttgta
     4201 agtggctatc tgcaacatac catttccata aaaacagctc tctatgcctc acttgtcatc
     4261 cagttgcttc ttctagtagg atttgccaca gttcgttttg actggattct catctttagt
     4321 accttggatg cctttttcgc aggtgttctc tctcctagat tgcaagaact cgtctttaaa
     4381 caaatacctg aggagtcaat gggagcagtt caatcctcta tcggtgccat tacggttgtt
     4441 ttacctagct tatttacaat agctttggta accattgcta ctagctttgg aactctggca
     4501 gttagctttg ttttattgct atttcttcta gttgcctttg ttatgcttct gaatattcgt
     4561 gaaagtattt agtggagaat ttctataata tatgtttgag actggattgc cagtcttttt
     4621 atgatggtca aaatcaacag acttttttct cttttctgaa tgtgttataa tagactgaat
     4681 ctaaaatagt acgaaacaat tgctaaaaca tttatagaaa ttaattttac tttcccaatc
     4741 gatttgttct catcttattt caatctgcta tataatagtc catgctgtgg ctggattctt
     4801 ttcagcctac ttatactctt cgaaactctc ttcaaaccac gtcagcttca ccttgccgta
     4861 ggtatggtta ctgacttcgt cagttctatc cacaacctca aaacggtgtt ttgagctgac
     4921 ttcgtcagtt ctatccacaa cctcaaaacg gtgttttgag ctgacttcat cagttctatc
     4981 tgcaacctca aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt gctctttgat
     5041 tttcattgag tattattaag acaatatgag gagaaagatg aaagaaaaag gattttagga
     5101 gggggcgcag gcagccatgc caacggctct tggctatgtc agtatcggcc tggcctgtgg
     5161 gactatcggt gcgccctatg tgacacctgt tgagatgggc ttgatgagtc tctttgttta
     5221 tgctgggagt gcccagtttg ccatgttggc actgattgtg gttcaagctc ctgtggcagc
     5281 tattgctatg acggtttttc taatcaactt gcgtctcttt ttgttgagtt tacacgcatc
     5341 gacttatttc cgtcatacca gtctttggta caatatcggt atgtctagta tcttgacaga
     5401 tgagacctat ggcgttttga tgggtgaatt ggcccataca gacaaggtaa atcctatgtg
     5461 gatgcacgga aacaatctta acagctatgt ggcttggttt gtggggacag tagtcggaac
     5521 ggctctgggt ggcctgctac caaatccaga aatctttggc ttggattttg ccctggttgg
     5581 gatgtttatt ggtatttttg cttcgcaatt tcagattatg caaagacgga ttcctgtccg
     5641 caatctgctc attatcctag cagttgttgc ggtgtccttc tttttgctct tgacagtggt
     5701 gtctcagtca ctagctgttc tgtttgcgac gctacttggt tgtagcatgg gggtggtttt
     5761 agatggtcag taagtatctt ttattagcag ttattttctc tggcttggtg acttggattc
     5821 tccgtatgat tcccttcatc ttagtcaagt ataagggctt gcctgcaatc gttgagcgtt
     5881 ttttgaagtt cttgcccgtt tccattatct ttgccttgat tctttcaagc gtagtgacag
     5941 gtaaggttgg gagccttcct caaattaaat ggctagactt cttagccgtc tttccaacag
     6001 cttgggtagc ctttcgctac cgcaatctag tcggaacagt tctctttgga gtggtcttga
     6061 ttgccatctt gcgtttggtc ttttaaatta cccataaaaa aaacttatca cagagataga
     6121 tatcatataa tggcgtaaat gctctttttc tgttaagatt ataaggtatt ctattttgga
     6181 ggaaatgaca tgaaaaaaat cgttaaatac tcatctcttg ctgccctagg acttgttgct
     6241 gcaggtgtgc ttgcggcttg ctcagggggt gctaagaaag aaggagaagc agctagcaag
     6301 aaagaaatca tcgttgcaac caatggatca ccaaggccat ttatctatga agaaaatggc
     6361 gaattgactg gttacgagat tgaagtcgtt cgcgctatct ttaaagattc tgacaaatat
     6421 gatgtcaagt ttgaaaagac agaatggtca ggtgtctttg ctggtcttga tgctgaccgt
     6481 tacaatatgg ctgtcaacaa tcttagctac actaaagaac gtgcggagaa atacctctat
     6541 gccgcaccaa ttgcccaaaa tcctaatgtc cttgtcgtga agaaagatga ctctagtatc
     6601 aagtctctcg atgatatcgg tggaaaatcg acggaagtcg ttcaagccac tacatcagct
     6661 aagcagttag aagcatacaa cgctgaacac acggacaacc caactatcct taactatact
     6721 aaggcagatt tccaacaaat catggtacgt ttgagcgatg gacaatttga ctataagatt
     6781 tttgataaaa tcggtgttga aacagttatc aagaaccaag gtttggacaa cttgaaagtt
     6841 atcgaacttc caagcgacca acaaccgtac gtttacccac ttcttgctca aggtcaagat
     6901 gagttgaaat catttgtaga caaacgcatt aaagaactct acaaagacgg aacgcttgaa
     6961 aaattgtcta aacaattctt cggagatact tatctaccag cagaggctga tattaaataa
     7021 tttcttgaaa tcatccattc tgagtaagat gggtgatttc tcagttttta gggatatagt
     7081 tttaaactat atatctgatt atctaataac aatttgtgaa atcaaacaaa ttgtgagata
     7141 ctagtacggt attattttta aggagaaaga atcatgaaaa tcaaaaaatg gcttggccta
     7201 gtagcccttg ctacagtcgc aggtttggct cttgcagctt gcggaaactc agaaaagaaa
     7261 gcagacaatg caacaactat caaaatcgca actgttaacc gtagcggttc tgaagaaaaa
     7321 cgttgggaca aaatccaaga attggttaaa aaagacggaa ttaccttgga atttacagag
     7381 ttcacagact actcacaacc aaacaaagca actgctgatg gcgaagtaga tttgaacgct
     7441 ttccaacact ataacttctt gaacaactgg aacaaagaaa acggaaaaga ccttgtagcg
     7501 attgcagata cttacatctc tccaatccgc ctttactcag gtttgaatgg aagtgccaac
     7561 aagtacacta aagtagaaga catcccagca aacggagaaa tcgctgtacc gaatgacgct
     7621 acaaacgaaa gccgtgcgct ttacttgctt caatcagctg gcttgattaa attggatgtt
     7681 tctggaactg ctcttgcaac agttgccaac atcaaagaaa atccaaagaa cttgaaaatc
     7741 actgaattgg acgctagcca aacagctcgt tcattgtcat cagttgacgc tgccgttgta
     7801 aacaatacct tcgttacaga agcaaaattg gactacaaga aagcactttt caaagaacaa
     7861 gctgatgaaa actcaaaaca atggtacaac atcattgttg cgaaaaaaga ttgggaaaca
     7921 tcacctaagg ctgatgctat caagaaagta atcgcagctt accacacaga tgacgtgaaa
     7981 aaagttatcg aagaatcatc agacggtttg gatcaaccag tttggtaata agaaacaggg
     8041 aggtgggaga gagaattcca cctcttgctt ttgtatagag cacggagtgt aaagaagact
     8101 attcgttcat agaaaggtag agagaatatg gtttttccta gtgaacaaga acagattgaa
     8161 aaatttgaaa aggatcatgt agcccagcat tattttgagg ttttgcgtac cttgatttct
     8221 aagaaatcag tctttgccca gcaggttgga ctcaaggaag tcgcaaatta tctgggtgag
     8281 attttcaagc gtgttggagc tgaagtggag attgatgaga gctatacagc gccctttgtc
     8341 atggcacatt tcaagagttc gcgtccagat gccaagacct tgattttcta taaccactat
     8401 gacactgtgc cagcggatgg ggatcaggtc tggacagagg atccttttac gctttcggtc
     8461 cgcaatggct tcatgtatgg gcgtggagtt gatgacgaca agggtcatat cacagctcgc
     8521 ttgagtgctt tgagaaaata tatgcagcac catgatgatt tacctgtcaa tatcagcttt
     8581 atcatggagg gagcggagga atcggcttca acagacctag ataagtattt ggaaaagcat
     8641 gcagacaaac tccgtggggc ggatttgttg gtctgggaac aagggaccaa aaatgccttg
     8701 gaacagctgg aaatttctgg tggcaataag gggattgtga cctttgatgc caaggtaaaa
     8761 agcgctgatg tggatatcca ctcgagttat ggtggtgttg tggaatcagc tccttggtat
     8821 ctcctccaag ccttacagtc tcttcgtgct gcggatggcc gtatcttggt tgaaggcttg
     8881 tacgaagaag tacaagagcc caatgaacga gaaatggcct tgctagaaac ttatggtcaa
     8941 cgaaacccag aggaagttag tcggatttat ggattggagt tgcctctctt acaggaggag
     9001 cggatggcct ttctaaaacg tttctttttc gagccagcgc ttaatatcga aggaatccag
     9061 tctggttatc aaggtcaggg tgttaagact attttacctg cagaagccag tgccaagcta
     9121 gaggttcgtc tggttccggg cctagaaccg catgatgttc tggaaaaaat tcggaaacag
     9181 ctagacaaaa atggctttga taaggtagaa ttatactata ccttgggaga gatgagctat
     9241 cgaagcgata tgagcgcacc agccattctc aatgtgatcg agttggccaa gaaattctat
     9301 ccacagggcg tttcagtctt gccgacgaca gcggggacag gacctatgca tacggtcttt
     9361 gatgccctag aggtaccaat ggttgcattc ggtctaggaa atgccaatag ccgagaccac
     9421 ggtggagatg aaaatgtgcg aatcgctgat tattacaccc atatcgaatt agtagaggag
     9481 ctgattagaa gctatgagta gagatattat caagttagat cagatcgatg tgacttttca
     9541 ccaaaagaag agaaccatta cagcggttaa ggatgtgacc attcacatcc aagaagggga
     9601 tatctacgga atcgttggat attctggagc agggaaatca acccttgtac gggtgattaa
     9661 cctcttgcaa aaaccatctg cagggaaaat taccattgac gacgatgtga tttttgacgg
     9721 caaggtgacc ttgacggcag agcagttgcg tcgtaaacgt caagatatcg ggatgatttt
     9781 ccagcatttt aacctgatga gccaaaagac agcagaggag aatgtagcct ttgcccttaa
     9841 acactctgga ctcagcaagg aagaaaagaa ggctaaagta gctaagttgt tggacttggt
     9901 tggtttggca gatcgtgctg aaaactaccc ttcacaacta tctggagggc aaaaacagcg
     9961 tgtggcaatt gcgcgtgcct tggccaatga tccaaaaatc ttgatttcag acgagtcaac
    10021 ttctgccctt gaccctaaga caaccaagca gattttggcc ttgttacaag atttgaacca
    10081 aaaattagga ttgacagttg tcttgattac gcatgaaatg cagattgtca aagacattgc
    10141 caaccgtgtg gcggttatgc aggatgggca tttgattgaa gagggaagtg tccttgaaat
    10201 cttctcaaac cctaaacaac ctttgactca agactttatc tcaacagcca caggtattga
    10261 cgaagccatg gccaaaatcg agaagcaaga aatcgtggaa cacttgtctg aaaacagtct
    10321 cttggtgcaa ctcaagtacg ctggagcttc aacagacgag ccacttttga atgaattgta
    10381 caagcattac caagtaatgg ctaatattct ctatgggaat atcgaaattc tcgatggtac
    10441 tcctgttgga gaattggtgg tggtcttgtc aggtgaaaaa gcagcgttgg caggtgccca
    10501 agaagctatt cgccaagcag gtgtacaact aaaagtattg aagggagtac agtaagatgg
    10561 aatcattgat tcaaacctat ttaccaaatg tctataaaat gggttgggct ggtcaggcag
    10621 gctggggaac ggctatctac ttaactcttt atatgacagt tctttccttc attatcggag
    10681 gcttcttggg gctagtggca ggtctctttc tcgtcttgac agcgccaggt ggtgtcttgg
    10741 agaataaagt cgtattctgg attttagaca aaattacctc aatttttcgt gcggttccct
    10801 ttatcatcct cttggcaatc ttgtcaccac tttctcactt gattgttaaa acaagtatcg
    10861 ggccaaatgc agcccttgtc ccactttctt ttgcagtctt tgccttcttt gcccgtcagg
    10921 tgcaggttgt cttggctgaa ttggatggcg gtgtcattga ggcggctcaa gcgagcggag
    10981 cgactttctg ggacatcgtg ggtgtttacc tatcagaagg tcttccagat ttgatccgtg
    11041 tgacgactgt gaccttgatt tcccttgttg gggaaacagc tatggccggt gcggttggag
    11101 ctggtggtat cggtaacgta gccatcgctt atggatttaa ccgctacaat cacgatgtga
    11161 ccatcttggc aaccatcatt atcattttga ttatctttgc aatccaattc ttgggagatt
    11221 tcttgactaa gaaattgagc cataaataaa aaagagccgc gtggctcttt ttaactgatc
    11281 agattttctg tgcaaatttt ttactcaagg cttgtccaat caaggcaccc actagggctc
    11341 cgatgacaat acttgcgata aatagaagga cagttccagg ggttggagcg accatgatac
    11401 ggtcgatata ttcttgggat tttcctcttg ccagaagagt agccatatag gctttgggcg
    11461 caatccacat aagcaagatt ggtcctgttg tactaaaggc gaaaataatg aaagaaagga
    11521 agttctttgt tttgtccttg tattttccta aatgagctac tccatctgct aggaggccac
    11581 agataattcc aggaaggaag gaaccagcac cgtgtttagt tcccaagaaa aagagggcaa
    11641 tgacaaggcc gatagtggta atggctccaa agcgcggaac ttttgcgact aggatcatat
    11701 agacgctacc gccgacaagg gcagtaaagg caggcgcata aaacatgttt ccagtttggt
    11761 caaagagatt gcccaaaagg acaccaatcc ccatgcagag gaagtaaaga actgcaaaaa
    11821 gcagtgtagt taagatagat ttcttcatga attgtctcct gataattttt tcacaattct
    11881 cattttacca tggtttggtg ggattgtaaa tggatactag aagtttttga aaacgcttga
    11941 aatatgatat aatggtttca tagttatttt taggggaata tcatgggtag atttttagac
    12001 tttgtcttta atcgtttctt tttagggatg attgcgacag ccttcttttg gctattaact
    12061 ttagcaggag ggattatcct tggtctagcg ccggctagtg ccaccttgat gagcttatat
    12121 gcagaacatg gttatagctt tcgggaatac agtttgaagg aggcttggtc tctttacaag
    12181 caaaattttg tctcaagcaa cctgattttc tatagctttt taggtgtggg tctagttttg
    12241 acctatggtt tgtatctctt ggtgcaattg cctcatcaga ccattgttca tttgattgcg
    12301 acccttttga atgtcctagt agttgccctg atctttttgg cttatacagt atctttaaaa
    12361 ttacaagttt attttgcctt gtcctatcga aatagtctca aattatcctt gattggcatc
    12421 tttatgagtc tagcagctgt ggccaaggtt ctccttggga ctgtgctact tgtagcaatt
    12481 ggttactata tgcctgccct gctatttttt gtaggaattg ggatgtggca tttctttatc
    12541 agtgatatgt tggaacctgt ctatgaaatc atccatgaaa aattggcgac aaaatagaat
    12601 gaagcagttt tggctacata cgcttctaag aacctatagt tcagtgatga tcattatcat
    12661 tgcgagtttt gcaatcttac tctcttacgc tgactgggat tcacgtgaaa aggaagccca
    12721 gagagtagcc cagcgtgtaa ctgctagaac actgagtgaa attgaatatt accatagaga
    12781 gtcaacccag atagctcagg ctttagttga aaatcaagct cgtattgagg gaatctataa
    12841 atactttagc cttagcatgc cagactattt ttactggcaa ttagagcgga aagcttcgcc
    12901 ttatatatca gtctctctgt atgaaaatgt tgatgacctc tatgttcgaa atgattttgt
    12961 aactggagtg gccattgctt ttcaagatta caaggaagtc tatgtttcta ctaaagacaa
    13021 acgtagtgga gaaaaaatca gggctgagga tttcaaacca gcaggaaata gttttgccat
    13081 tccagtgtca gatccagtgt cagatcaaga cttaggagtg atttacatct ccttggatcc
    13141 tgctgtttta taccatgcca ttgataatac tagaggtcat actccgatgg cagtaacagt
    13201 gacctcacct tttgatacgg agatttttca tatgggtgag acagttgata aggagagtga
    13261 aaattggcta gttggcttaa cttctcatgg ttatcaggtt caggtggcag ttcccaaaaa
    13321 ctttgtttta caaggaacgg tgaccagctc tgctttgatt gtgggcttga gccttctctt
    13381 tattgtcatt ctttatctga ctttgaggca gacctttgct aattatcaaa agcaggtagt
    13441 ggatttggtg gattccatcc aagctattgc ccaaggacaa gaaggtcttc gcattgatac
    13501 gcttgaaaag gatcaggaat tgctcctaat cgcggagacg accaatgata tgttggatcg
    13561 attggaaaag aatatccatg atatttacca gttagaactc agtcaaaaag atgccaatat
    13621 gcgggccttg caggcgcaaa tcaaccctca cttcatgtat aatacgctgg agttcttgcg
    13681 tatgtacgca gtcatgcaga gtcaagatga gttggcagat atcatttatg aattcagtag
    13741 tctcttgcgt aacaatattt ccgacgaaag agagaccctc ctcaaacagg aattagaatt
    13801 ttgccgtaaa tacagctatc tctgcatggt tcgctatccc aagtccattg cctatggttt
    13861 caagatagat ccagagttag agaatatgaa gattcccaag tttaccttgc aaccgctggt
    13921 agaaaactat ttcgcgcatg gtgttgacca caggcggaca gataatgtga ttagcatcaa
    13981 ggctcttaaa caggatgggt ttgtggaaat tttggtggtc gataatggta gaggaatgtc
    14041 ggctgaaaag ttggcaaata tccgagaaaa attaagtcag aaatattttg aacaccaagc
    14101 cagctacagt gatcaaaggc agtctatcgg gattgtcaat gtacacgagc gttttgtgct
    14161 ctattttgga gaccgctatg ccattactat agagtctgca gagcaagccg gtgttcagta
    14221 tcgtattaca attcaagatg agtagaaagg gagaaaatgt ataaagtatt attagtagat
    14281 gatgagtaca tggtgacaga aggtctgaag cgtttgattc cctttgataa gtgggatatg
    14341 gaggtcgtcg caacagccag tcatgccgat gaagctctag aatatgttca ggaaaatcct
    14401 gtcgatgtca tcatttccga tgtcaatatg ccagacaaaa cagggcttga tatgattcgg
    14461 gagatgaaag agatcttacc agatgctgcc tatatcctgc tctcaggtta tcaggagttt
    14521 gattatgtaa aaagagcaat gaaccttagt gtggtggact atttggtcaa gcctgttgat
    14581 aaggtagagc tgggaaatct gctggagaag attgcaggtc agctcggcga gagagggaag
    14641 aaaagtcaga ctcttagtca agaattagac gaggctggat ttgttagtta tttaggggat
    14701 aaggagaatt ggtggatagg tctatccaag gaaaaacaag gttccttcac cattccctac
    14761 tatgtcttgg gccaagactg gcagattttc atttctgacc aacccctaga tggtttagtc
    14821 gttacacctt ttgaagctcc ttatcaagaa cactttgaac gctggaagct gaatgctgag
    14881 aaaaccctct tttacggttc tgtaaatttg cagcagtctg agagtctctt tgcctattac
    14941 gaaccgattt atagggttat tattcaggga aatctcaatc aaatcgtaga agagttaaat
    15001 ctcttggaga aggtagttct tgaaaatacg ccgcgtgttc cgattactaa acagcttttt
    15061 attcagtttg tcatggatgt tttccattta tttgaacatc tcaaagctga tgacctgaca
    15121 gacatcgtca aaaccattca tgctattcaa tccttcgatg aattggttcc ttatatcaag
    15181 gaaactctga gcagcttttt cggtcaatac cgtatgaatg aaaatgtggt cagtgtgctg
    15241 gaagtcattg gtcgtgatta ccaaaaagag ctttccctca aggatatcag taaggctctc
    15301 tttatcaatc ctgtctatct agggcagttg attaagcgtg aaaccgattc gacttttgca
    15361 gagttactaa acaaacaacg tattaaggct gcccaacaac ttttgctttc aactagtgac
    15421 agcatcgaag atatttgtta tgctgttggt tacagtaacc ttggatattt ctataaagtt
    15481 ttccgaaaat tgtgcggaaa atcgccaaaa gcctaccgaa aacaggtaga aactatacta
    15541 taagatttgt attcctttac aaaaggtgct ataatattat caggaggtcc tatcatgaaa
    15601 aagaaaccaa tttatctatg ggtcttgcta atcttgtctg cccttatttc agttccgtct
    15661 ctgtttggaa ttgtgagtcc cttgcctagt aaagaagccc ttcgtgctgc tcagaaacaa
    15721 gttgcagggg tcaatgctca gcaattagaa gaccagctta attataccta tagagtagca
    15781 gaagcatctc attctatttt taatgttgct ttgattgtgc tatctactat tttagtggtc
    15841 gtagcgattg tcttccttgt tcgtaaaaat ttgcaatatg caaactatac ttacgttggc
    15901 tatgttttgt tagctattat tggttcgatt tatggctatg ttggtttaca agacgctgtg
    15961 cagttggttc aagatgagag catgcgtttg acagtgagta ttggttcaaa agctgttagc
    16021 attttctata tcgtcatcaa tgttctcttc ctagcccttg tcttctataa gatgtggcga
    16081 caacagaaag ccttggcaga agaagaggaa acagaagagc ttacctaagt attgacaaaa
    16141 aagaactatc aagatacaat cttggtagtt gttttttgat tatagataaa atcatggagg
    16201 tacatatgaa gacaatttct ttagtttata ttagtttgag tggcaacact gaaagttttg
    16261 tgacgcgctt gaaagactat ctcttgtccc agtacaaagg gattgaggtt caaaagattc
    16321 atatcaagga tttggtcaag gaaggcaaaa atttctatga aatggaccat ccctatgtcg
    16381 cttttttgcc gacctatctc gaaggtggga atggcgtgga taacggagat gttgagattt
    16441 tgacgacacc ggtgggagat tttattgcct atggtaacaa tgctagtaag tgttttggtg
    16501 tggttgggtc aggaaatcgt aactttaata accaatactg cctaacagcc aagcaataca
    16561 gtcaacgctt tggcttccct gtattggctg actttgaaat gcgaggcatg ctggaagata
    16621 tcaaacatgt cgcagctatt atcgcagatt tgtatgagtt ggaaaaggag aattaatact
    16681 caatgaaaat caaagagcaa actaggaagc tagtcgcagg ttgctcaaag cacagctttg
    16741 aggttgcaga tagagctgac gtggtttgaa gagattctcg aatagtataa agttcttata
    16801 agatgtggtt gaagttgttc aactgttttt tgagtataaa cagtctttaa gaacgtaaaa
    16861 aagcataagt tcaggtcttc aaaaccttgt tcttatgctt tttatcatag aaatatttgc
    16921 tgaattttct cttgaaatga aaccttatct tcatctcgcg cttgtccctt accctgcttt
    16981 tccgattgcg agtgcgtaga taaagaagat ggcgaagcca aagaggaaaa tcaatcccgc
    17041 aatggcaagg tgtttgagcc aagaaggaag atttttgttt tcacgtgtta ccaaggcgta
    17101 attcaaaaga gcaaagaaag gtgttgtcag gaaagagcca atcatggcaa agcggagcat
    17161 ggttgaaacc tgaccagcga agaacttgat aatgacgata ccgatgatag cagtgatggt
    17221 catccagatg ttcaaagatt tacgattgtc ctctttttga ctgattagca gtcggagaga
    17281 ttcctgatta acgcgagaat agccatcgat aacagttata actgttccaa agatacagag
    17341 gaaggcaata aaggtaatca agtaacggga ccattcgcca agaacagagg catacatgcc
    17401 cacgaattga gagatgtatt tggctgaagc agcttcaacc gcctgccctg taggatactg
    17461 aatcagtgct cccagtgcca caaagaagac ggctaggata gctgttccaa tataaccagt
    17521 gttaaagtca aacagagcgt cttctgtgtt aaagttgacg gtctttctct tttcagttga
    17581 ccaaagtgaa ttgatggctg aaatttcaat aggagccggc atccatccta ggagggagac
    17641 gatgaagggc agagctgcca tttgccaagg tgtcttctcg acaaaatcag aactgtattc
    17701 tggatgcttg accgccgcaa tgataactgc aagaacagtc gcaatggtta aggcagacat
    17761 aatccatttg accatgccgt ctaaaagttt gtagcctcca aagagtagca tagcccaaat
    17821 gattgcaacg agaatgaggg accactgagt aatgctaagt ccaatcattg ggaaggcact
    17881 ggcgatgata gctgagcaca gaatggcaac accagccgtg ttgaccatag ccgaaaagac
    17941 attgagaata aagaaaatcc agagatagag ttttcctttt tcggcataac cttcaaccaa
    18001 agtctttcca gtatcagctg tgtattcagc accaaaacgg aaaaatggat atttaaagac
    18061 attggctaag atgaccaaga gaagtagaga ccaaccgtaa gaaccgccag cttgagttga
    18121 ggatacaatg tgggaacctc caacagcggc agttgccatt aggattccag gtcccattgc
    18181 ttttagcttg cttgcccagg ttgattgatt taaggaaata gcttgcgaca tgataaatac
    18241 tcctttttga attttcagaa taatctgtat atgcaatcta tattctacaa aaaattttgg
    18301 aaaaatgcaa gaatattttg aaaaaagata gaaaatttac aaagaaaatc tgaaataact
    18361 gttatgatat agcagattga aactagaata gtacac
//
LOCUS       DAVLSL010000030        17017 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLSL010000030 DAVLSL010000000
VERSION     DAVLSL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.030"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..80
                     /locus_tag="VXN30_000794"
     CDS             <1..80
                     /locus_tag="VXN30_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator (MGA)"
                     /protein_id="HEV1357454.1"
                     /translation="IYSNNINTVSLIYLLNAMMFIRLDE"
     gene            441..1442
                     /locus_tag="VXN30_000795"
     CDS             441..1442
                     /locus_tag="VXN30_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1357455.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            1593..2510
                     /locus_tag="VXN30_000796"
     CDS             1593..2510
                     /locus_tag="VXN30_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1357456.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            2521..3411
                     /locus_tag="VXN30_000797"
     CDS             2521..3411
                     /locus_tag="VXN30_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1357457.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            3440..5056
                     /locus_tag="VXN30_000798"
     CDS             3440..5056
                     /locus_tag="VXN30_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1357458.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            5066..6385
                     /locus_tag="VXN30_000799"
     CDS             5066..6385
                     /locus_tag="VXN30_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1357459.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(6596..6668)
                     /locus_tag="VXN30_000800"
     tRNA            complement(6596..6668)
                     /locus_tag="VXN30_000800"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6633..6635),aa:Lys,seq:ctt)
     gene            complement(6933..8204)
                     /locus_tag="VXN30_000801"
     CDS             complement(6933..8204)
                     /locus_tag="VXN30_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1357460.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            8474..8713
                     /locus_tag="VXN30_000802"
     CDS             8474..8713
                     /locus_tag="VXN30_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357461.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            8878..9057
                     /locus_tag="VXN30_000803"
     CDS             8878..9057
                     /locus_tag="VXN30_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357462.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            9194..9373
                     /locus_tag="VXN30_000804"
     CDS             9194..9373
                     /locus_tag="VXN30_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1357463.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGK
                     LNKYTERGIGKQAGL"
     gene            9410..9862
                     /locus_tag="VXN30_000805"
     CDS             9410..9862
                     /locus_tag="VXN30_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1357464.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFGYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            10153..10623
                     /locus_tag="VXN30_000806"
     CDS             10153..10623
                     /locus_tag="VXN30_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1357465.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            10642..11730
                     /locus_tag="VXN30_000807"
     CDS             10642..11730
                     /locus_tag="VXN30_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1357466.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLMLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            11711..12139
                     /locus_tag="VXN30_000808"
     CDS             11711..12139
                     /locus_tag="VXN30_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1357467.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYKLMPIEEFLNSKQAVPQLQGRLRDYLEESL"
     gene            12136..12609
                     /locus_tag="VXN30_000809"
     CDS             12136..12609
                     /locus_tag="VXN30_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357468.1"
                     /translation="MITLVRAGAEDLETIIAIQRASFKAVYEKYHDEYDPYLEDCERI
                     KWKLVERPNSYYYFVKEDEENIGFLRVQTNAELTKAWLGTAAILPQYQGKGYGSKGLS
                     LLEKEFPTITQWDLCTVLQDAGMVAFYEKNGYRQIHIEAEKEGMDMVYMKKLIEK"
     gene            12747..13697
                     /locus_tag="VXN30_000810"
     CDS             12747..13697
                     /locus_tag="VXN30_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1357469.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            13699..14442
                     /locus_tag="VXN30_000811"
     CDS             13699..14442
                     /locus_tag="VXN30_000811"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1357470.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            14453..16249
                     /gene="pepF"
                     /locus_tag="VXN30_000812"
     CDS             14453..16249
                     /gene="pepF"
                     /locus_tag="VXN30_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1357471.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(16309..16986)
                     /locus_tag="VXN30_000813"
     CDS             complement(16309..16986)
                     /locus_tag="VXN30_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1357472.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
BASE COUNT         5258 a         2985 c         3800 g         4974 t
ORIGIN      
        1 tcatctattc aaataatata aacacggtct cactcatata tttgttaaat gccatgatgt
       61 ttattcgatt agatgagtaa ccaaaaataa caaaaaaacg ctatttcgcg tttgaactgt
      121 acctcaaaag ttagacagaa aaaatctaat ttttggggtg tttttgttaa gaaaagacct
      181 tcaatcattt aagcaaaatg tacatctaga tttatagccc tctaaaactc ttgctttttt
      241 gtagaagata cgaaaaaagc cataaaacca aggcttttcc tgttttatgt agatgtttac
      301 acatgaaaat tgaagtattt tcaaaagcta attggagtga ttttattcat gatgggataa
      361 atgatctggc caaaaatgat tgagaaatag ttgcttaaaa agcatttgca tgatactata
      421 tattgaaagg aggtgataat atggtagaac aaagaaaatc aattaccatg aaagatgttg
      481 ctttagaagc aggagttagt gttggaactg tttcacgtgt aattaataaa gaaaaaggca
      541 ttaaagaagt aactttgaaa aaagtggaac aagcgattaa aactttgaat tacattccag
      601 attactacgc tagaggaatg aaaaaaaatc gaacagaaac gattgcaatc attgtaccaa
      661 gtatctggca tcccttcttt tcagaatttg ctatgcatgt ggaaaatgaa gtctataaga
      721 gaaataacaa attactctta tgttctatca atggtacaaa tagagagcaa gactatctgg
      781 agatgttgcg tcataataaa gttgatggag tggttgccat tacctatagt ccaattgaac
      841 attacttgac gtcaggaatt ccctttgtta gtattgaccg cacatactca gatattgcca
      901 ttccttgtgt ttcatcagat aatgatgcag gtggaagaga agcagctaaa caattaatta
      961 gtaaaggatg tcagcattta gcctttgtgg gcggacataa tacaaccatt aatgaaacca
     1021 aacgtcgtag aatttcattt gaaaagtatg tccaagagca taaaatacct cttagtattt
     1081 ttgatttgga tgagacagtt actgactatt atggaaagct agagagtttc ttggaagaaa
     1141 attcttctat agatggaatt tttacaatca acgattttac agctttggat gtgattgaaa
     1201 tactagaaaa aaatggcaaa cgtattcctc atgatgtcca aattattgga tacgatggaa
     1261 ttaaaatggc tggagataga gattatctac tttcaactat tgaacaacca ttggaagaaa
     1321 tggcaaaaga agcggttcgt attttgtttg atataattga tggaaagact gttaatttgc
     1381 agacaatcct accagtaaaa tttgttgaag gaaaaacaac aaaaaatgaa aataagtctt
     1441 gacagaaagc gctatcaatg atagaatgaa ttcagataaa aagatttatt tttaaaacaa
     1501 aaatgaaacg tttcaaaaaa agaaataaag agacagcgcc aagcgctatc ttttctagaa
     1561 aaaaaatgaa acgtttcaaa aaggaggttg ctatgaatag caaagcgaag caagtttctc
     1621 tttgggaaag aatcaagaaa caaaaactct tgttattgat gactgtccct ggtttagttt
     1681 taacctttat ctttaaatac atccctatgt atggggtttt aattgcattt aaagattaca
     1741 atcctttaaa aggaatttta gggagtgatt ggattggttt ttctgagttt acaaaattca
     1801 tatcctctcc caactttggt atcttgttag ccaacacatt aaaattaagt atctatggtt
     1861 tattgcttgg ctttttacca ccaatcattc ttgctattat gctcaatcaa ctcttgagtg
     1921 aaaaagtcaa aaaacgaatt cagctcattt tatacgcacc aaactttatc tcagttgttg
     1981 ttattgtcgg tatgattttc ctcttctttt cagtgggagg accaatcaac aattttcttt
     2041 ctatgtttgg aatgaaggct gacttcttga caaatccaga cttctttaga cctttataca
     2101 tctttagtgg tatctggcaa ggaatgggct gggcttcaac gctctacacg gcaacattgg
     2161 taaatgtaga tccagcctta gtagaagcag cccgactgga tggagccaat atcttccaac
     2221 gaatctggca cattgatatt ccagctctta agcctattat ggttatccaa tttgttttag
     2281 ctgcaggtgg aattatgaat gtcggatatg aaaaagcatt cttgatgcag acatcgttaa
     2341 atttgccaac ttctgaaatt atctcgacat atgtctataa agttggtctt gtatcaggag
     2401 actattctta ctcaacagcg gttggtttgt ttaatgcagt gattaacgta gtattgcttg
     2461 ttgcagttaa ccaaatcgtt aaacgcatga ataatggtga aggaatttaa ggaggaaagt
     2521 atgaaaaatt cgattatgga tacaaaattt gatagacgta tcttactctt aaataaaatc
     2581 attattgtct ttatcgtttt gatgactttg cttcctttac tttatatcgt cgtagcatcc
     2641 tttatggatc ctaaggttct ggttagtaga gggattagct ttaatccagc cgattggact
     2701 gtagaaggtt accagcgtgt attcagtgac caatctattc taagaggttt tatcaattct
     2761 ctactatact cttttggatt tgcagcttta acagtattgc tatctgtgtt tacagcttat
     2821 cctctttcta agaaagactt ggttggacgt cgttggatta actacttctt gattgtgact
     2881 atgttctttg gtggtggttt agtcccaact tacttgctcg taaaagaatt gggaatgctc
     2941 aatactccat gggctatcat tgttccaggt gctgtcaacg tttggaatat tattcttgct
     3001 agggcctatt tccaaggatt gcctgaagaa ttagttgaag ctgctgtcat tgatggtgca
     3061 aatgatttac agattttctt caaaatcatg cttcctcttg caaaaccaat tatgtttgtt
     3121 ctcttccttt atgcttttgt aggacagtgg aactcatact ttgatgcaat gatttatatc
     3181 aaggatccaa acttggaacc attgcaactt gtacttcgta aaattctcat tcagagccaa
     3241 ccaggtcaag acatgattgg agcacaagcg gctatgaatg aaatgaaacg tttagctgaa
     3301 ttgattaaat acgcaactat tgtcatttcc agcttgccat tgattgttat gtatccattc
     3361 ttccaaaaat actttgataa aggaattatg gctggttcac ttaaaggata aaaaaagaaa
     3421 aaataaaagg agttttctca tgaaattcaa aacattctca aaatcagcag ttttgttgac
     3481 agctagttta gcagtacttg cagcctgtgg ctcaaaaaat acagcttcaa gtccagatta
     3541 taagttggaa ggtgtaacat tcccgcttca agaaaagaaa acattgaagt ttatgacagc
     3601 cagttcaccg ttatctccta aagacccaaa tgaaaagtta attttgcaac gtttggagaa
     3661 ggaaactggc gttcatattg actggaccaa ctaccaatcc gactttgcag aaaaacgtaa
     3721 cttggatatt tctagtggtg atttaccaga tgctatccac aacgacggag cttcagatgt
     3781 ggacttgatg aactgggcta aaaaaggtgt tattattcca gttgaagatt tgattgataa
     3841 atacatgcca aatcttaaga aaattttgga tgagaaacca gagtacaagg ccttgatgac
     3901 agcacctgat gggcacattt actcattccc atggattgaa gagcttggag atggtaaaga
     3961 gtctattcac agcgtcaacg atatggcttg gattaacaaa gattggctta agaaacttgg
     4021 tcttgaaatg ccaaaaacta ctgatgattt gattaaagtc ctagaagctt tcaaaaacgg
     4081 ggatccaaat ggaaatggag aggctgatga aattccattt tcatttatta gtggtaacgg
     4141 aaacgaagat tttaaattcc tatttgctgc atttggtata ggggataacg atgatcattt
     4201 agtagtagga aatgatggca aagttgactt cacagcagat aacgataact ataaagaagg
     4261 tgtcaaattt atccgtcaat tgcaagaaaa aggcttgatt gataaagaag ctttcgaaca
     4321 tgattggaat agttacattg ctaaaggtca tgatcagaaa tttggtgttt actttacatg
     4381 ggataagaat aatgttactg gaagtaacga aagttatgat gttttaccag tacttgctgg
     4441 accaagtggt caaaaacacg tagctcgtac aaacggtatg ggatttgcac gtgacaagat
     4501 ggttattacc agtgtaaaca aaaacctaga attgacagct aaatggattg atgcacaata
     4561 cgctccactc caatctgtgc aaaataactg gggaacttac ggagatgaca aacaacaaaa
     4621 catctttgaa ttggatcaag cgtcaaatag tctaaaacac ttaccactaa acggaactgc
     4681 accagcagaa cttcgtcaaa agactgaagt aggaggacca ctagctatcc tagattcata
     4741 ctatggtaaa gtaacaacca tgcctgatga tgccaaatgg cgtttggatc ttatcaaaga
     4801 atattatgtt ccttacatga gcaatgacaa taactatcca agagtcttta tgacacagga
     4861 agatttggac aagattgccc atatcgaggc agatatgaat gactatatct accgtaaacg
     4921 tgctgaatgg attgtaaatg gcaatattga tactgagtgg gatgattaca agaaagaact
     4981 tgaaaaatac ggactttctg attacctcgc tattaaacaa aaatactacg accaatacca
     5041 agcaaacaaa aactagaggt tgattatggg agataagaaa tacacagtag aaaaagccaa
     5101 tcgttttata gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt
     5161 ttcagctgag attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata
     5221 ccatctcttt tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca
     5281 tgctaaaagt aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca
     5341 agattatgac cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg
     5401 gctcatgtac actgggcata tcgaagaaaa aaccggtgtc cgccaagtgc aaaatatggc
     5461 attttcagat gacgggattc actttgaaaa gatttcccaa aatccagttg taactggaac
     5521 agacttgcca gatgagttga ttgctgctga tttccgtgac ccaaaactct ttgaaaaaga
     5581 tggacgctat tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct
     5641 actagggtcc gataacctag tagaatggca gttcgaatcc atctttttaa aagggggaga
     5701 acaccaaggc tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct
     5761 tattatgtca cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc
     5821 gcttttgttc acgggtaagg tagattgggg agaaaaacgt tttatcccag aatcagttca
     5881 agaaattgat catggccaag acttctatgc gcctcaaaca ttgttggaca atcaaaatcg
     5941 tcgtatcctg attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga
     6001 acacaaatgg gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact
     6061 aagacaattc cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta
     6121 ccacttagga aatgatatag attatcttga atttggttat gacagtaatg cgcagcaagt
     6181 ttacattgat cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag
     6241 tcgacggtat gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat
     6301 cgagattttt gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc
     6361 agctgagcta tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct
     6421 ttctaaaata gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta
     6481 tttgtaaaat tggtgttgga ttatgattta aactagtttt ctaaagaatt tgaaaaaaat
     6541 tttatttaag caaaaaaaag ccctatatta taagactttt cctgttgtat ttaaatgccc
     6601 cctacaggga tcgaacctgt gacccacgga ttaagagtcc gctgctctgc cagctgagct
     6661 aaggaggcaa agaaaaaagc tgtattggtg ccgaaacttc acggtttgta ttgaacccgc
     6721 gcaattaagc aggtgggcaa ctcgctctaa ctgaagctgt ttccgtgtga gacggcctac
     6781 atgctgttag aagacttttg tttccctaat aatacaaaaa atagtcggtc aacacttaag
     6841 tgtgaagtcg tacaccacag cgtttctata tttatatgat accacttttt caaaaaaaat
     6901 caagaggaaa gtgcaatttt ttgaaaagga tttcagattt ttcgcaaatg gtcgatagct
     6961 tcctttcttt gagccaaagc ccgttcatat ttaccagtat cttctgcttg gaaatagtga
     7021 tgattacgaa ttttttctgg cagatagtct tgctttaccc aatttccagg atagttgtgt
     7081 ggatagagat agtcttgggc attccccagt tccttgcttc cactgtagtg cccatcacgt
     7141 aggtgtcgcg gaataggcaa gtgccctgat gttttgaggt cagcaagtgc cttatccata
     7201 gctacatagg ctgagttgga ttttggagaa agggccaaat caatcacgac attggcaatg
     7261 agaatgcggg cttctgggaa cccaatcttc tgggcagcat ccagagcagt cacggtatga
     7321 atctgggcct caggattggc taagccgata tcttcatagg cgataacagt caagcgacga
     7381 gcgagactag gcagatcccc agcctcaatc aagcgggcag tatagtggag actggcatcc
     7441 acatctgagc cacggataga cttttgcagg gcagatagaa catcatagtg accgtctcca
     7501 tccttgtcca tagtgatgta gcttctctga agactatttt ccatgatgtc taaggtgatg
     7561 tggcgaatgc cctcgtcatt ctcaggggta gagagaacag ccaagtccag tgagttaaag
     7621 gcagagcgaa ggtctccgtt tgtagaggtt gcgatgaaat ccagcgcatc ctcatctagt
     7681 tctattggaa aatcaaaacc acgttcaggg ttacttagag ctatctgcag ggcctctttg
     7741 acgtcttggt tagacagagg ttccaactcg aaaatttgaa cacggctacg aatggcagga
     7801 gtgacagaga agaaaggatt ttcagtcgtt gctccaatca tgatgaccag tccactttcc
     7861 aagagaggca agaggaagtc ttgcttggtc ttatctagtc gatgaatttc gtctagcaat
     7921 aggacgagac caccagaaaa tttagcttct tccgagattt cttgcagtcg ctttttacta
     7981 tcaactgtcg cattgaaagt tcgaaaggca tacttggtcg ttccagcgat ggcagaggca
     8041 atactggttt tgccgattcc aggagggcca tatagaatca tggaggacag gcggttggct
     8101 tccaccatgc ggcggatgat ttttccaggt ccgaccagat gctcctgacc gatgacctgg
     8161 tcgatggttt tagggcgcat gcgaagcgcg agattgtctg gcatagcagt cctttctaac
     8221 gtggatttga gtagaatgac tcatctacta tatcttacta tgtccattct agcctattag
     8281 ttgtgatatt tcaatggttt tgtaataatc agggagtgag atagaatctt ttttactatt
     8341 taatttaatg aggagaggta tcagcatgag agatttgatt ttccttaaag ctcaaaatag
     8401 gatagttgag cctaaagaag tggaagtgct gacttgagaa ataatattgc taaatgtcat
     8461 tgtatgaact ataatgaaaa gagaggtgat aagcaatgga aatgatggac caagtcagga
     8521 aattttgatt tttacaaaac aaattcgtca ttggattctt agtgatcaag ttatttcagg
     8581 gaaaagaaaa cttttcttcc gggaagatac tccaaaagag attttagatt tgtacgaaaa
     8641 cattaaatct aaacttgatt ttgcttacca agaagtttat tctaataatg gattgaaaaa
     8701 atatgaaaaa tagaataatt gatgtttttg aagtagtaaa tcgcctcctt gtaataactg
     8761 ttgaaaatcc tgatttcgaa gacttgagag ttaatcatac tcaatgaaaa tcaaagagca
     8821 aactaggaaa ctagccgcag gctgtacttg agtacggtaa ggcgacgttg acgtggtttg
     8881 aatttgattt tcgaagagta tcagtttgta aaaattggag ataaaaaata tagagtacgc
     8941 agtggtccta tgattcattc aactccgcct caatctgtct tagatcgaga tacttttaca
     9001 attgactata cagatgatga attgctcgat aaagaagcag tttttactac tcattagaaa
     9061 aataggtctg aataaattgg tttcccttag aaccacatct aggggctttt atgtataata
     9121 gaataataag tatcactcct ttacaatacg tgtaacacgt gttataatgg tgttgtcagg
     9181 aggtaaaagc gctatgccta tgacacaaaa agagatggtt aaactcttta cggcccatgg
     9241 ttggataaag actagaggcg gtaaaggctc ccatattaaa atcgagaagc aaggggaaag
     9301 acctatcaca atccttcatg gtaagctgaa taagtacact gaaagaggga taggaaagca
     9361 agctgggttg taaaattcag tccctgcttt ttgatatgga ggtagtgaaa tgttagttac
     9421 gtatccagct ttattttatt atgacgacac agacggaaca gaagcgactt attttgtcca
     9481 tttcccagat tttggatact cagctacaca aggagagggg atttctgagg ctttggctat
     9541 ggggtcggag tggctaggga taactgttgc agatttgata gagagtgatg gagagttacc
     9601 tcagccatca gatatcaata gcttgtcttt aattgataat gatcctttta aagatgatga
     9661 agatttcgtg tcaacctatg accttgataa atcttttatt tctatggtat ctgttgatgt
     9721 atcagaatac ctaggaagtc aggaacccat taaaaagact ttgaccatac caaaatgggc
     9781 agataagttg ggacgagaaa tgggacttaa cttttctcag actttgacag acgctattgc
     9841 agataagaaa gttcaagcct aagattaagc gtggtatcgt ggtaatagtc aataaaaaag
     9901 cacgtccact tgtgctagtt actcgcctat tgattttata gatttattag ccctttcgcg
     9961 ggctttttat attgattttt atgagaaatg aagaaatcag acttttaaga aaatcattga
    10021 taccaagggt ttaaatgagg taatatggta taattaggac ataaaataat tttgtggtaa
    10081 gatggtagta tctattttag catatttccg agcaatcggg gcgattaaag agtcgcatag
    10141 aaagaggaca aaatggcaac atacggattt ttagatattt tagaggaaga gttggacaag
    10201 aactttccct ttgactttga gattagttgg gacaagcgta accacgcggt agaagtgagt
    10261 tttctattgg aagcgcaaaa tgctgcaggt gtggagatgg tggatgaaga cggagaggtt
    10321 tcatcagatg acattctttt tgaggaagcg gtccttttct acaatcctgc taagtcaacc
    10381 gtcaatgcag aagactattt aacggttatc ccttatctac ctaaaaaagg attttctcgc
    10441 gagtttttag cttattttgc ccttttcctt aaagatactg ctgaggttgg gctagatacc
    10501 ctcatggact ttttggaaga cccagaagca gaggaatttg tcatggaatg gaaccaagaa
    10561 gtctttgaag aaggaaaagt tggcttggaa gagggagaat tttatcctta tccaagatac
    10621 taggagttgg ttggaggtgt tatgaagaaa attgggactt atttggttta tgtgctagct
    10681 gttgtcttta ttatgctagc ttttgcttgt ggaacaatcg catttgcaga gttggggtat
    10741 tccgcagttt tagcctttac ttttggttat gccttcgctc ttctaagcat gtatttaatc
    10801 tttattcttc atgagctggg tcatgctttt tgtggctact tgacaggcta tcggctggtg
    10861 gcttttggat taggacattt tattttgacc aaaaagtcag gcaggtttca tcttagtaga
    10921 acagccattc tgaaaaatgt tggtgctcaa tacattggtt taaaagagga tgaaagcgat
    10981 caaagaatca tcctgatgct ttcaggaggc ttgatggttc atctcggctt gatattattg
    11041 gcgatagtgt ttggattttt gacaagaagc tggtattttg cagggacttg gatttttctt
    11101 aatttatctt tcttcctaaa taacattttg ccagtcgaca tcaccgatgg agcaaaaatt
    11161 tgggaattac tacaacaccc tgaaaatacc aaatacgcct acctgatgtt gaggcattct
    11221 gcccagacct tgctagctcc tcaagaatat gatttaaaag actttgttca agcagttcct
    11281 gaggatgcac gagggagttt tgcggatggt gtattaggta tgcaaggtga agtttccatt
    11341 ttggaaggga aggaagaagt agctaaacag cagtttcaag cattacttga gaggacaact
    11401 actccgatga tgcagacagt tgcccaacta tccctgcttc atatcgcctt gttggaagga
    11461 gattttgagc aagcggagca atatgctagt attcgacagg tcaaatcgtt tctgtctctc
    11521 aaactgtcca atcttcaagt gatacaagct tggtatcaat ttaaggtaaa gaaagatgta
    11581 gatcgaactc gtaaggctct caagattgct agacagaaaa tggatagtag tcgaatgttg
    11641 agggacgaga aacgctatta tgaaaacttg ttagcagagc tggaaaagga aatgttagaa
    11701 ggaactgaag atggaactag aaatttctga tttccaaggt tgcaagattg ccctcttttg
    11761 tggggataag ctactgacga ttttgcgtga tgacaaggac gatatccctt gtcccaacat
    11821 gtgggagttg ccaggtggtg gccgtgaagg ggacgaaagc ccttttgaat gcgctcggcg
    11881 tgaagtttat gaggaactgg gaattcattt agatgaagac tgtctgcttt ggagcaagat
    11941 ttatcctagc gtaattttta aaggcaagaa atcggtcttt atggttggtc agctaagaca
    12001 agaacaattt gacaatatca tcttcggaga tgaaggacag ggctataaat tgatgcctat
    12061 tgaggaattt ctcaattcta aacaggcagt gcctcagttg cagggaagat tgagagatta
    12121 tttggaggaa agtttatgat aacacttgtt cgagcgggag cagaggattt agaaactatt
    12181 attgcaattc agagagccag ctttaaggct gtttatgaaa aataccatga tgaatatgac
    12241 ccttatctcg aggactgcga acgaattaag tggaaattag tcgagcgtcc caatagctat
    12301 tactactttg taaaagaaga tgaagaaaac atcggatttt tacgagttca gaccaatgcc
    12361 gagttaacga aagcttggtt gggaacggca gcgattttgc ctcagtatca aggaaaaggg
    12421 tatggatcta aaggtttaag cttactggaa aaagaatttc caaccatcac acaatgggat
    12481 ttgtgtacgg tgttacagga tgcaggcatg gttgcttttt acgagaaaaa cggctatcgt
    12541 caaatccaca ttgaggctga aaaagaaggc atggatatgg tctacatgaa aaaattgata
    12601 gagaaataga aaggatggtt cggttaaatt tctaaactga acccgcccta aacacggtgc
    12661 caaaaagata aacttctctt agacacaaac gtcttcagag agtttcctat tttggctttg
    12721 tgttttacgg gcttggtatc ttaataatgg aaacatggca agagttaaaa gttacagtga
    12781 agcgtgaggg agaggagttg gtttccaatc tcttgattga gttaggtgcc caaggggtag
    12841 caattgaaga cagcatggac tatgtgggaa atgtggaccg attcggtgaa attttcccag
    12901 aggtggagca acaggaagaa atcgtagtga ccgcctacta ccctgaaacg gttgatgtag
    12961 cagcagttga agcggatttg caggctcgtc tggcagaatt gaccgacttt atggacctag
    13021 gagaagtcaa gatgggcacg actgccttgg ctgaggaaga ctgggctgac aactggaaga
    13081 agtattatga accggcacgc atcacccatg acctgaccat cgtaccgtca tggacggact
    13141 atgaggcaac agcaggagaa aagattatca agctggatcc tggtatggcc tttgggacag
    13201 ggacccaccc aacaaccaag atgagcctct ttgccttgga acaggttctt cgtggtggcg
    13261 aaacagtgct agatgtaggg actggttcag gggttctttc tattgcaagc tcactcctag
    13321 gtgccaagga gattttcgcc tatgacttgg atgatgtggc ggttcgtgtc gctcaggaaa
    13381 atattgagct caacactggt atggaaaata tccatgtagc agcaggagac ttgcttaagg
    13441 gagttgagat tgaagcagat gtcatcgtgg ctaatatctt ggcggatatc ctcattcatc
    13501 taatagacga tgcctatcgt ttggtcaagg acgaaggcta cctgatcatg agtggcatta
    13561 tcaaggataa gtgggacatg gtgcgcgagt cggctgaatc agctggattt ttcctcgaaa
    13621 ctcacatggt tcaaggggaa tggaatacct gtgtctttaa gaaaaccaag gatatctctg
    13681 gtgtgattgg aggctagcat gcagcagtat tttgtaaaag gcagtgctat ctctcctgtc
    13741 actatcgagg acaaggaaac cagtaagcat atgtttcagg ttatgcgctt gaaagaagag
    13801 gatgaggtta ccttagtctt tgatgatggc atcaagcgct tggcgcgcgt gctggatatg
    13861 gaaaatcgtc agtttgagtt ggtccaagaa ttaaatgaca atgtggaact accagtccaa
    13921 gtgaccatcg catctggatt tcccaaggga gacaagctgg agttcatcgc tcaaaaagta
    13981 accgaactgg gggctagcca aatctgggcc tttcctgcag actggtcagt tgccaagtgg
    14041 gatggcaaga aattgggtaa aaaagttgaa aaactagaaa aaattgccct tggagcagcc
    14101 gagcaaagca agcgtaatat tgtaccaagt attaaacttt tcgagaaaaa agcagatttt
    14161 ctagctcagc tggaccagtt tgactctatc atagtagcct atgaagaatc agctaaagaa
    14221 ggagaagccg ctgcgctctt acaagcggtc tctggtcttg aaacaggagc caaactgctc
    14281 tttatctttg gaccagaagg cggtctctca cctgcagaaa tcgaaagttt tgaagctaag
    14341 ggagccgttt tggtaggcct aggtcctcgt attttgcgag cagaaacagc accgctttac
    14401 gccttatcag cccttagtgt tttagtagaa ttagagaaat aagaggaaga aaatggaaca
    14461 aaaacaccgt tcagaatttc cagagaagga actctgggac ttaacagccc tataccaaga
    14521 ccgtgaggat ttcttgcgtg caatcgagaa agctcgcgaa gacatcaacc agtttagccg
    14581 tgattacaag ggcaatcttc acacttttga ggatttcgag aaggcctttg cggaattgga
    14641 acagatctac attcagatga gccatattgg caactatggt tttatgcctc agacgacgga
    14701 ctatagcaat gacgaatttg ccaatattgc ccaagctggg atggaatttg aaacagatgc
    14761 cagcgtagcc ttgaccttct ttgacgatgc cttggtggta gcagacgagg aagtcttgga
    14821 ccgtttgggt gaattgcctc acttgacggc agctattcgt caggccaaaa tcaaaaaagc
    14881 ccactatcta ggggctgatg tggagaaggc cttgaccaat ctcggtgaag ttttctatag
    14941 tccgcaggac atttatacta agatgcgagc tggggatttt gaaatggctg actttgaagc
    15001 ccatggcaag acctacaaaa acagctttgt cacctatgag aatttctacc aaaatcacga
    15061 ggatgccgag gttcgtgaga aatccttccg ttccttctca gagggacttc gtaagcacca
    15121 aaatacggct gcagcagcct atctggctca ggtcaagtct gaaaaactct tggctgatat
    15181 gaagggatac gactctgtct ttgattatct tctagctgaa caagaagtgg accgtgtcat
    15241 gtttgatcgc cagattgacc tcatcatgaa ggactttgca ccagtcgctc agagatacct
    15301 caagcatgtt gccaaggtaa atggtcttga aaagatgacc tttgcagact ggaaattgga
    15361 cttggacagc gccctgaatc ctgaagtgac tattgacgat gcctatgatt tggtcatgaa
    15421 gtcggtagaa cctttggggc aagaatattg tcaggaagtt gctcgttacc aagaagagcg
    15481 ctgggtggac tttgctgcta acagtggcaa ggattccggt ggttatgcgg cggacccata
    15541 tcgcgtacac ccttatgtac tcatgagctg gacaggccgt ttgagcgatg tctatacctt
    15601 gattcatgaa atcgggcatt ctggtcaatt catcttttca gacaatcatc aaagttactt
    15661 caatgcccat atgtcgacct actatgttga agcaccgtca accttcaatg aattgctact
    15721 cagtgattac ttggagaacc agtctaatga cccacgtcaa aaacgcttcg ctctggctca
    15781 tcgcttgaca gacacctact tccataactt tatcacccac ctcttggaag cagccttcca
    15841 gcgtaaggtg tatacattga ttgaagaagg ggagaccttt ggagcaagca aactcaacag
    15901 cattatgaag gaagttttga cagatttctg gggagatgct attgaaattg atgatgatgc
    15961 agctttgact tggatgcgcc aagctcacta ctatatgggc ttgtatagtt acacctactc
    16021 agcaggacta gtcatctcga cagctggtta ccttcatctt aaaaactcag aaactggagc
    16081 tgaagactgg cttaatctcc tcaaatcagg tggtagcaag acaccacttg agtcagccat
    16141 gattatcggt gctgatattt caacagacaa accactccgt gataccatcc aattcttgtc
    16201 tgacacagtt gaccagatta tcgcctacag tgctgagttg ggggagtaag gggatagaga
    16261 ggttgttata gaatgggtat ggacagttat ttgcttaaaa gtccatgttt aaatccccca
    16321 ttccttttcg aggaattctt tgcccttctc tgtaatggta agtgaacgtg gcgtttgtgc
    16381 agggatgacc aattgttttt cactgaaaaa ttctaaaaga gcattgccga gagctccgcc
    16441 gacatgattt ttacgttcgc tccaatccaa acatttaggg caaaatttcc gttttttctt
    16501 tttcaattca tccgtatcaa tgtgtaattt tctttggaaa taagacttcc cagcttcagt
    16561 gagttggaag tctttttctg attcgctgag gagattttct tgcaagagat aattggtaat
    16621 ggaaatgcca agctcaccag caatatggtc atagcaggtg cgagcgacct ttaattcctt
    16681 tttgtgaatg tgttgtttat aacttttgac atcacgctga ggagagtaga agctgagaaa
    16741 ttccagcatt tctgcaatgg cagcactttt taaagaataa taatgaaaac gtccataggt
    16801 ttgtagagtg gttacctgcg cttcggtaag taatttgagg tgatagctga cagtatgttg
    16861 cttgattttg gtaaagctgg caatttcgtt aaccgtgtga gcttttccat ccattagaat
    16921 atctaaaatg tgcatccgag aagggtctga taagagtttt gtgatgatga aaatatctgg
    16981 ttccataaaa attttctcca aatttcatat atcgatt
//
LOCUS       DAVLSL010000031        16861 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLSL010000031 DAVLSL010000000
VERSION     DAVLSL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.031"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            145..345
                     /locus_tag="VXN30_000814"
     CDS             145..345
                     /locus_tag="VXN30_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357473.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            776..1099
                     /locus_tag="VXN30_000815"
     CDS             776..1099
                     /locus_tag="VXN30_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1357474.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            1157..3100
                     /gene="thrS"
                     /locus_tag="VXN30_000816"
     CDS             1157..3100
                     /gene="thrS"
                     /locus_tag="VXN30_000816"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1357475.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(3149..4123)
                     /locus_tag="VXN30_000817"
     CDS             complement(3149..4123)
                     /locus_tag="VXN30_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1357476.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(4116..4793)
                     /locus_tag="VXN30_000818"
     CDS             complement(4116..4793)
                     /locus_tag="VXN30_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1357477.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            4949..6022
                     /locus_tag="VXN30_000819"
     CDS             4949..6022
                     /locus_tag="VXN30_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1357478.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEVFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(6113..6415)
                     /locus_tag="VXN30_000820"
     CDS             complement(6113..6415)
                     /locus_tag="VXN30_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357479.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(6483..6920)
                     /locus_tag="VXN30_000821"
     CDS             complement(6483..6920)
                     /locus_tag="VXN30_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1357480.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(6996..7838)
                     /locus_tag="VXN30_000822"
     CDS             complement(6996..7838)
                     /locus_tag="VXN30_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1357481.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            7950..8600
                     /locus_tag="VXN30_000823"
     CDS             7950..8600
                     /locus_tag="VXN30_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1357482.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            8777..9423
                     /locus_tag="VXN30_000824"
                     /pseudo
     CDS             8777..9423
                     /locus_tag="VXN30_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            9554..9968
                     /locus_tag="VXN30_000825"
                     /pseudo
     CDS             9554..9968
                     /locus_tag="VXN30_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9987..10401
                     /locus_tag="VXN30_000826"
                     /pseudo
     CDS             9987..10401
                     /locus_tag="VXN30_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10440..10883)
                     /locus_tag="VXN30_000827"
     CDS             complement(10440..10883)
                     /locus_tag="VXN30_000827"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1357483.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(10913..13135)
                     /locus_tag="VXN30_000828"
     CDS             complement(10913..13135)
                     /locus_tag="VXN30_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1357484.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            14131..14760
                     /locus_tag="VXN30_000829"
     CDS             14131..14760
                     /locus_tag="VXN30_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1357485.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(14883..16775)
                     /gene="pepO"
                     /locus_tag="VXN30_000830"
     CDS             complement(14883..16775)
                     /gene="pepO"
                     /locus_tag="VXN30_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1357486.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         4873 a         3905 c         3180 g         4903 t
ORIGIN      
        1 aatatttcta ccaagaaaac taaccatgat tctagtgaaa aaaaatcttc tttgccaaca
       61 aatttacttt cttgttttaa acatgctata ataatcatag caagagatct aagttgtctg
      121 tttttttaaa acgaggtgat tatcatgcgt agattctatt cccatctccc ctactatctg
      181 gtcatattat tcttttattg gccactttat gagttgttct tactagttgt ttctgacccc
      241 cttacactca aggaactcta tataaacaat cttctcttct ttacacctct ggtaatcttg
      301 attgtatcgt tactctatag ctaccgtttc cgtttctcac tttgatggtt agttggtaac
      361 ggactgctct tttactttac tatcataacc tttggtgagt ttatactaat ttacttgcta
      421 atctatgaaa cagttgctct ggtgggcatg gattctggta ttagcatcaa gcatattcta
      481 caaaaaatga aaaacaaaaa actttcacaa aatccttgaa aaatctcaca atcatgctat
      541 aataatccat agagacaagt cacttagtcc ctttctacta gagagtgcgt ggttgctgga
      601 aacgcatagg aagtctaaac tgatactact cttgagtttt ttatgaaaac ataaaacggt
      661 ggccacgtta gagccaatca gaggtgtccc tctcttttga ggtacataaa tgaaggtgga
      721 accacgttgc gacgtccttt cgaggatgtc gcattttttt attaggatac taattatgga
      781 gttgcaagaa ttagtggagc gcagttgggc aatccgacaa gcttatcacg aactggaagt
      841 taagcatcat gattccaagt ggacggtaga agaagacccc ttggctttat ctaatgatat
      901 tggaaatttc caacgactgg tgatgacaaa gcaaggacgc tactatgatg aaacacccta
      961 cacactggaa caaaaacttt cagaaaatat ctggtggcta ttagaacttt ctcaacgttt
     1021 ggatatagac attctgacgg aaatggaaaa cttcctctct gataaagaaa agcaattgaa
     1081 cgttaggact tggaagtagt ctgatgataa aaaatcaatg cttagaaact atgaaataat
     1141 aaaaaaggag aacatcatga ttaacattac tttcccagat ggcgctgttc gtgaattcga
     1201 atctggcgta acaacttttg aaattgccca atctatcagc aattccctag ctaaaaaagc
     1261 cttggctggt aaattcaacg gcaaactcat cgacactact cgcgctatca ctgaagatgg
     1321 aagcatcgaa attgtgacac ctgatcacga agatgccctt ccaatcttgc gccactcagc
     1381 agctcacttg ttcgcccaag cagctcgtcg tcttttccca gacattcact tgggagttgg
     1441 tccagccatc gaagatggtt tctactacga tactgacaac acagctggtc aaatctctaa
     1501 cgaagacctt cctcgtatcg aagaagaaat gcaaaaaatc gtcaaagaaa acttcccatc
     1561 tattcgtgaa gaagtgacta aagacgaggc acgtgaaatc ttcaaaaatg acccttacaa
     1621 gttggaattg attgaagaac actcagaaga cgaaggcggt ttgactatct atcgtcaggg
     1681 tgaatatgta gacctctgcc gtggacctca cgttccatca acaggtcgta tccaaatctt
     1741 ccaccttctc catgtagctg gtgcgtactg gcgtggaaac agcgacaacg ctatgatgca
     1801 acgtatctac ggtacagctt ggtttgaaaa gaaagacttg aaaaactacc ttcaaatgcg
     1861 tgaagaagct aaggaacgtg accaccgtaa acttggtaaa gagcttgacc tctttatgat
     1921 ttcacaagaa gtgggacaag gtttgccatt ctggttgcca aatggtgcga ctatccgtcg
     1981 tgaattggaa cgctacatcg taaacaaaga gttggcttct ggctaccaac acgtctacac
     2041 tccaccactt gcttctgttg agctttacaa gacttctggc cactgggatc attaccaaga
     2101 agacatgttc ccaaccatgg acatgggtga cggggaagaa tttgtccttc gtccaatgaa
     2161 ctgtccgcac cacatccaag ttttcaaaca ccatgttcac tcttaccgtg aattgccaat
     2221 ccgtatcgct gaaatcggta tgatgcaccg ttacgaaaaa tctggtgccc tcactggcct
     2281 tcaacgtgta cgtgaaatgt cactcaacga cggtcaccta ttcgttactc cagaacaaat
     2341 ccaagaagaa ttccaacgtg cccttcagtt gattatcgat gtttatgaag acttcaactt
     2401 gactgactac cgcttccgcc tctctcttcg tgaccctcaa gatactcata agtactttga
     2461 taacgatgag atgtgggaaa atgcccaaac catgcttcgt gcagctcttg atgaaatggg
     2521 cgtggactac tttgaagccg aaggtgaagc ggccttctac ggaccaaaat tggatatcca
     2581 gattaaaact gcccttggaa aagaagaaac cctttctact atccaacttg atttcttgtt
     2641 gccagaacgc ttcgacctca aatacatcgg agctgatggc gaagatcacc gtccagtcat
     2701 gatccaccgt ggggttatct caactatgga acgcttcaca gctatcttga ttgagaacta
     2761 caagggtgcc ttcccaacat ggctggcacc acaccaagta acccttatcc cagtatctaa
     2821 cgaaaaacac gtggactacg cttgggaagt agccaagaaa ctccgtgacc gcggtgtccg
     2881 tgcagacgta gatgagcgca atgaaaaaat gcagttcaag atccgtgctt cacaaaccag
     2941 caagattcct taccaattaa ttgttggaga caaagaaatg gaagacgaaa cagtcaacgt
     3001 tcgtcgctac ggccaaaaag aaacacaaac tgtctcagtt gataattttg ttcaagctat
     3061 cctagctgat atcgccaaca aatcacgcgt tgagaaataa gagtctagca taaaagcctc
     3121 caatctggag gctttttctc atctattttt actcaaggac taagttcact tgagcaaact
     3181 gaatccgcac tgtcgttcct tttccgacct cagactcgat acgaatctgg tgccccagtt
     3241 cttcagaaat tttcttagat agataaaggc caagtccaga ggactgctgg gtcaaacggc
     3301 cattgtatcc tgaaaagcca cgttcaaata ctcggaggac atcactgttt tttatcccga
     3361 ttcccgtatc tttgatacaa agctcttggt catccatata aatctccaga ccaccttcct
     3421 tggtgtactt gagactgttt gagatgattt gctcaataac cactagcagc cactttttat
     3481 ccgtcacgat ttctttatca aggtcatgta gattgacatt taagcctttt tgaataaaga
     3541 aaagagcata tttacgaatt atttccttga ccaagtcctc aatttgaacc tgctttaaga
     3601 ccaaatcatc atggaaactt tctaaacgca ggtactgtaa aactaggttg gtataggagt
     3661 cgattttgaa aatttcctgt tctagctgct gcttcagttg gcggtcgacc acttctgcaa
     3721 ctaagagttg actggctgca atgggggtct ttatctgatg gacccacaag gtatagtaat
     3781 ccagcaaatc cgtcagtttt ctttctgctt ttgacctctg ctgatagagt tccatctcac
     3841 gcgcttctaa tttttctgct aaagctattt ccaaaggaga cttggcttcc ctctctccat
     3901 agagaagttc ctggcgatag acctgcgttt ccaccaatat gtcccaagtg aaaaataaga
     3961 tggttacaaa gcaacacaag aagaaaaagt agaggaagta aattcctaga ctggcaaata
     4021 aaaactgaaa gagtaagaca agaaatgcca aagaaagcag atagataaaa agacgactac
     4081 gggagcgcag ataggctaga aaaaattgtt tccaatcaag catgcttcaa tccgtaccct
     4141 attcctttct tggtctcgat aaatcctacc aatccctgct cctccaactt tttacgcaaa
     4201 cgagccacat tgacagagag ggtattatca tcaatgaaaa agtcactgtt ccaaagttcc
     4261 cgcatcaggt cgtcacgtgc tacgatgttg cctgcatgct caaataacac gcgtaaaatc
     4321 tggaattcat tcttggtcaa attcaagact tgcccttgat aatgtaaatc catggatttg
     4381 gtattgagga taacaccagc atattccagc aaactctcat cacgcccaaa ctcataggaa
     4441 cgacgcaaca agccctgaac cttagctaaa agaacctgct ggtcaaaagg cttggtcaca
     4501 aagtcatccg cccccatatt gattgccatg acaatatcca tagcctggtc tctcgaagaa
     4561 agaaacatga taggtacctt ggaaatcttg cggatttcct gacaccagtg ataaccatta
     4621 aacaagggca aaccaatatc catgaggacc agatgaggtt ccgactgaac aaatagactc
     4681 aaaacttcca taaagtcttc taccaggacc acttcaaatc cccattcaga gagcattttc
     4741 ccgacctgtt gacgaatgac ctgatcatct tctattaata aaatcttgtg catgcgcttc
     4801 tccttttcta ttattataac agatttttcc atgccagatg gtctgaaact gaatttgaaa
     4861 tagcctgttt ttagccagta caaacaggca atgctactag ctaatttgag ggaaatttgc
     4921 taagataaat aaaaagaaag gagctcttat ggccaatatt tttgactatc tgaaagatgt
     4981 cgcatatgat tcttattacg accttccctt gaatgagtta gacattctaa ccttaataga
     5041 aatcacctac ctctcctttg ataatctggt ctccacactt cctcaacgtc ttttagatct
     5101 agcacctcag gttccaagag atcccaccat gcttactagc aaaaatcgcc ttcaattatt
     5161 agatgaattg gctcaacaca agcgcttcaa aaattgcaaa ctctcccatt ttatcaacga
     5221 catcgaccct gaactgcaaa agcaatttgc ggctatgact tatcgtgtca gcctcgatac
     5281 ctatctgatt gtctttcgtg ggacagatga cagtatcatt ggctggaagg aagttttcca
     5341 cctgacctat atgaaggaaa ttcctgctca aaagcacgcc cttcgctatt taaagaactt
     5401 ttttgcccat catcctaagc aaaaggttat tctagctggg cattccaagg gaggaaatct
     5461 cgctatctat gctgctagcc aaattgagca aagtttgcaa aatcagatca cagcagttta
     5521 tacatttgat gcacctggtc tccatcaaga attgacacag actgcgggtt atcaaaggat
     5581 aatggataga agcaagatat tcattccaca aggttccatt atcggtatga tgctggaaat
     5641 tcctgctcac caaatcatcg ttcagagtac tgccctgggt ggcatcgccc agcacgatac
     5701 ctttagttgg cagattgagg acaagcactt cgtccaactg gataagacca acagtgatag
     5761 ccagcaagta gacacaacct ttaaagaatg ggtggccaca gtccctgacg aagaacttca
     5821 gctctacttc gacctcttct ttggcactat tcttgaggct ggtattagct ctatcaatga
     5881 cttggcttcc ttaaaggcgc ttgaatacat tcatcatctc tttgtccaag ctcaatccct
     5941 cactccagaa gaaagagaaa ccttgggtcg ccttacccag ttattgattg atactcgtta
     6001 ccaggcatgg aaaaatagat aatactcttg aaaattaaat gtatacaaaa caaaagacct
     6061 agaatacata ctttcatgtg cattctaagt ctttttaaat agaatctaat agtcaataaa
     6121 aatcaaagag cattgagaga taatggggct tggaacgtcc ctctcgcttc aacaaaatga
     6181 ccccattata gattaaaaag atgccactta gaaaaagcaa aaaaggaagt aagacaaagg
     6241 caaatacata aaaagctaac tgaacattct cgtatccatt tttataaaaa aggtaggata
     6301 gataaaaata acttgaaatg agggataata aaaataatac tggattccac aaacttctat
     6361 tatccttcca aaatgacact ataaaggcta atacaattcc tataacgaga tacatttctt
     6421 actcctttaa tagctacatt ttatcataat tatccaaaga aaaaagaggg catttatccc
     6481 tcttaatcct tcatctgact ctctgcatcg gccacgactt tttctagact ggtttgacca
     6541 agttctgcct ccatagtcaa ctgaattctc tccaattttt gatccaaaac atcatgaata
     6601 tgagctccta cagggcaatt tggattcgga ttgtcatgga aactgaagag ttgacctgtc
     6661 ttaccaagac attcgaccgc ctgataaaca tctaaaagac taatatcctt aaggtccttg
     6721 acaatctctg ttccgcccgt tccacgcgct actgaaatca gctctgcctt cttcaactgg
     6781 gacaagatct ttctgataat gacaggattg accccgacac tagcagccag aaaatcactg
     6841 gtcaccttgc tttccttccc ctcgagggca atgattatca gcatatgagt cgcaatggta
     6901 aatctacttg gaatttgcat cctcttctcc tttttacgag gctaccctgc ctctactctt
     6961 ctttttctat tattataccc tttttagttg taatgtcaat cgttaccact tttcaaccag
     7021 tcgtctaact cccgatcgca gccctctttc tgagccaatt ctctcaaaaa ttcctgatga
     7081 tgagtatggt ggatcccatt gaccagactt tcatagtaaa cctcaaaata gggaagtctc
     7141 aggtctttag ccagctgcaa ttccgctgcc acatcgtagt ctacccgtcg gaagtccata
     7201 tctaccaggc ctttgtcatc aaactccaaa atcatatact gggcccgcaa gtccttccgt
     7261 agctgagcgt ccaaaaagaa aggttggcca atcgaacccg gattgacaat caattgccca
     7321 ccagtcccgt aacgaagcaa ctgctggtga atatgtccat aaacagcaat atcacaggga
     7381 ggatgagtca ccaagcggtc aaactcctct tgtttgccag tatgaatcaa ctctcgcccc
     7441 cagttcttat caggcagatg atggctaatt cccaccgtca aatccccaaa ctgacgatga
     7501 atttgaagag gttgattgtg gagcacttca atttcttcta gggaaatttc ctctaaaaca
     7561 tactggcact ggcgcaagag atagcgttga ctggggcgag tactgtccaa ttccttacgg
     7621 acaccatgcc aaagactgtc ttcccagttt cccaaaactc tagccgtaat cggtagttga
     7681 tccaacaagt ccaaaatcct tctacgtcct gtccctggca tgagaatatc tcccaaaagc
     7741 cagtattcat ccactcctat ctgctgagca tctgccaaaa cagcctccaa ggcggtggta
     7801 tttccatgaa tatctgaaag aagagctatt ttcgtcatat ccatctcctc gttttttctc
     7861 ttgcaataag tataacataa aaagtcacag ctagagaaat ctagcttttt ttgatatact
     7921 agataaagat attagacaag aggaaacgaa tgaccccaaa caaagaagac tatctaaaat
     7981 gtatttatga aattggcata gacctgcata agattaccaa caaggaaatt gcggctcgca
     8041 tgcaagtctc tccccctgcc gtaactgaaa tgatcaaacg aatgaaaagt gaaaatctca
     8101 tcctaaagga caaggaatgt ggctatctac tgactgacct cggtctcaaa ctggtctctg
     8161 agctctatcg taagcaccgc ttgattgaag tttttctagt tcatcattta gactatacaa
     8221 gtgaccagat tcacgaggaa gctgaggtct tggaacacac tgtctctgac ctgttcgtgg
     8281 aaagactaga taaactgcta ggtttcccta aaacctgccc ccacggagga actattcctg
     8341 ccaagggaga actcctcgtt gaaatcaata acctcccact agctgatatc aaggaagctg
     8401 gcgcctaccg cctgactcgg gtgcacgata gttttgacat tctccattat ctggacaagc
     8461 actcacttca catcggtgac cagctccaag tcaagcagtt tgatggcttc agcaatacct
     8521 tcactatcct cagtaacgac gaggatttac aagtgaatat ggacattgca aaacaactct
     8581 atgtcgagaa aatcaactaa tttctcaagt cccctaccaa ccctgaaagt tttattttca
     8641 gggttttatt tctatcattt gattttactt tcatttggtg gtagagtatt tgaaaaaaag
     8701 aaaggtagtt taacctatga aaaaatcact aaaaattttt gctacatcta aatgatttga
     8761 cctcttcggg gttgctttgg tcgttgggat tgcgattgca tctggttatc tcaactcacg
     8821 cctcgataaa tttgtagact ggggactatg gacagctctt gtaccctttg gattgatttc
     8881 cgtaaccaac gttgggattt ccatgttgtc cactcgtttc acggggaaat taagcaaatg
     8941 gggaaattac tttggtattg ttaataccat tttgtccggt gctattgatt atatccttgg
     9001 aaataaggcg gccatcatta cctatcccgt caccttcctc atttatacct ttgctataaa
     9061 gaaatggaaa gcttcgcaag aaggcagacc caaccaaatg agccaaaaac aggtaaaatt
     9121 ggcggccatc atcatttcca tcatcgcctt cctctttgcc tttgtgacca actatatcgg
     9181 atatgggggc aagatgaatc tccttgccta cgtaacaact attgcctttg cactgtccct
     9241 cattgccaat gctttgaatg cattggcaat gagggacagt ggggcttttg gttgatttac
     9301 aatttcgttc agctgacaaa agctggtatt caaggaaact tcgccaatat cggtaaatac
     9361 atcttttata tcctcaatgc aatcggagct ttatttgtct ggaatgatga agaagttaga
     9421 taatagaagg aaactcaaat aaagaatact agtcaactta agagaatctc ccctctgcta
     9481 tttttatcat tttagttgta actcccattc ttttagagta gaatgaagtt agataaaaga
     9541 gggaggtctt gtcatgaaaa aactctatag aatccatttt atagcaattg cagttattga
     9601 tttgctacta tttgcatttt ttatcattag actcgagaca tcttttgagt ggctcttgct
     9661 ctctggttta attttcttcc ttgctcaagg actcctgcta tttctcttga tcgtccgact
     9721 caaacatcaa ttcgctgaga tttatcctca aatcaataaa aagattcgct tctactattt
     9781 aggggttctc accattgatt ttctattttt ttgttctctt agccttcatt agttctcagc
     9841 gtttttcatc tcttatgtca attatcactg cttgccattc tactttttat tatatgacag
     9901 ctgactacct aagagaaaac tatccagact tttacgacaa acacatctct ttatgggagt
     9961 gtctctaagg aaaaggaggt tttatcatga aaaaaatcat cttcatcaaa accattcaac
    10021 tccttgtcat tgatggaatc atgctggcat ttttgacatt taaaaggggg gcttacttgg
    10081 gactggattt tgatttatag cggttggctc attttctttc atcctgtgct attgacctat
    10141 ctttcaaacc aactttgtga ccactttagt caactctatt cccagattag accgagattc
    10201 tggcgttttg ctttacaaat tctcctatgg gatagcctga tgattctctc cttggtgtct
    10261 ttaagtgata ttccactttt ccttcaggga actctcctca tcctaggaca tctcatccct
    10321 tcctatcgca tctgccaaag cctgaaaaga gacttccccc aagcatatca agaaccgatt
    10381 tctttttgga gtattttatg atagatgaga aagaccaagc cgactgggct tggtctttct
    10441 tatctctttt tagtatctag gataatggta acaggtccgt tattaaccag ctcaacctgc
    10501 atatctgctc caaagatacc tgtctgaacg ggcacttctt gcgctaattt ttgattgaaa
    10561 gcatcataga agtctgatgc catatcaggt ttagctgccc ctgtaaaggc tggacgattg
    10621 cctctcttag tatccgcaaa gagggtaaac tgagaaatag agaggatttc tccttcaata
    10681 tctttgacag acaggttcat cttgccttct gcgtctgaaa aaatccgcat attgaccagt
    10741 tttctcacag catagtccaa atcttcctct tggtcctctg gtccaacacc aactagcaat
    10801 aaaagtccct gattgatttt tccctgaatc tggccttcta tactcacttg ggctttttta
    10861 acccgttgga taatgatttt cataatagcc tttctagtaa gagctaggac aactagccgt
    10921 tggtccgttt gacagagtaa acttctggca cactcttaat tttatcgaca accgtggtca
    10981 gtgtagagag gttggcaata ccgaaggaca catggatatt agcaaacttc atatccttgg
    11041 ttggttgggc attgaccgtt gaaatattct tggttgtatt tgaaagaact tgcagtacat
    11101 cgttcaacag tcctgtacgg ttgagaccgt agatatcgat atgggccata tactccttat
    11161 ttgagctaga gtactggtct tcccattcca catcaaggag acgttgctcg tagttttctt
    11221 gggcacgcag gttcatacag tccacacggt gaatagccac accacgaccc ttggtaatgt
    11281 agccaacaat atcgtcacca ggcacggggt tacaacactt agcaatccgc actaggagac
    11341 cagaagcacc ttcaataacc actcccccct catgcttgac cttgagagtt tctttatttt
    11401 caaccttgac ctcgccacct ttgacaagct cctctgcctc agccttggcc ttggcacgct
    11461 cttcctcacg gcgttccttt tcagtcagac ggttaaagac ggtaatcgca ccgatttccc
    11521 caaaaccaat ggccgcaaag agggagtctt ctgtcttgta actggtcttt tgcagaactt
    11581 gatccatgtg gcgcttgtcc ataaatttat ttgccacata gccattttct tggaactgag
    11641 ccatcagcat ctcacgaccc ttgttgacag acaattcctt atcttggttt ttaaagaact
    11701 ggcgaatctt attgcgcgcc ttgctagtct tgaccatatt gagccagtca cggctaggtc
    11761 caaaggagtt cgggttggcg ataatttcaa cctgatcccc tgtctttaac ttggttgtca
    11821 gtggaaccat gcggccattg accttggcac cagttgcttt ttcaccgacc ttggtatgga
    11881 tttcgtaggc aaaatcaatc ggtcctgaat ctttgggaag ggaacggaca gctccatctg
    11941 gggtaaaaac gtaaatctcc tcagccagat agttttcctt aacagagtcc acaaattcct
    12001 tagcatcatc agcctggtct tggagctcca tcatctcctt gatccagttc attccaatag
    12061 ctgattcctt gctgttaact tgccccttta tacctttctt ataagcccag tgagccgcaa
    12121 ccccgtactc agccacctcg tgcatttcct tggttcgaat ctggaattca atcggccctt
    12181 ttggcccata aacagtcgta tggatagact gataaccatt ggccttgcgg ttggcaatat
    12241 agtctttgaa gcgacctggc atcggtttcc aaaattcatg cacgtaacca agcatggcat
    12301 aaacatcact ttgggtatct aaaatacaac gaatagcaat cagatcatag atttcctcaa
    12361 accgttttct cttgtcctgc attttgcgga aaattgagta aatatgcttg ggacgaccat
    12421 aaatcttccc tttcaagtga cgttctgtcg tatactcctc taattttgtg actacctcat
    12481 ccaccaaggc ctcacgctcc ctgcgctttt ccttcatcat atgggtaatc ttgtaaaact
    12541 ccgttggatt gagataacgg aaagacaagt cttctaattc ccatttgaca ctggaaatcc
    12601 ccaaacgatg ggcaagtggg gcatagattt ccatggtttc tttggaaata cgctcctgct
    12661 tgtcttttcg aagatgtttc agggtccgca tattgtgcaa gcggtcagac agtttgacca
    12721 aaataacgcg gatgtcctca gacatggcca tgagcatctt gcgatgattt tccgctaatt
    12781 gctcctcgat tgatttgtac tcgaccttgc caagcttggt aactccgtca acaatcaccc
    12841 gcacatcagg accaaactct ctttccaaat cgtccaaggt cgcatctgta tcttccacca
    12901 catcatgcaa gaatccacaa gctactgtta cagcatccag ctttagctta gctaaaatac
    12961 ctgccacttg gatagggtga atgatataag gctcgcctga tttgcgatat tgaccactgt
    13021 ggcattcaac agcatagacc aaggccttat ggacaaaatg aacatcctct tccgttaaat
    13081 attctttggt taaagcgaca acttcttcgc ctgttaaatt cacttctttc ggcatctcta
    13141 ctctccaatt cttcctacca ttttatcact tttttaagaa tatgaaaact agattggaac
    13201 agaataagaa aaaaataatt caaaattgct tgataattct gaattatcgg cccataatat
    13261 actacgaagt tagattttaa acttaggtga tagaaggaga gatagaagaa cggaaaccat
    13321 attgtaaccc aaagactttc tgatttcccc aattccattg aagatacgaa agataaacgg
    13381 tggaactcgt atcacataca ctggtacctt gactggattt tggaattaat actcaatgaa
    13441 aatcaaagag caaactagga aactagccgc aggttgctca aagcactgct ttgaggttgt
    13501 agataagact gacaaagtca gtcacatata tacggcaagg cgacgttgac gcggtttgaa
    13561 gagattttcg aagagtataa aaatcctcaa gatactttct tctatccttt agtttataag
    13621 gagaatacct atgaaaaaaa ctgctatttc tatctttgct ctcctaatgt taggagtttg
    13681 ctgcctgttc ctattcagcc agcaaagcta taaaaaacag tcgttcaata ctatgctaac
    13741 gaccagaacc tgcccagtag gataacttat agtgaatata gcgacaaacg agaagccaac
    13801 tacggtagca ctctaaacat cacgtctatc aaacaagcta atgacggagt ttatgcaacc
    13861 tatgaagggc aattgacacc tttccaatat tgataaattg ataaccagcc tgtcttcatc
    13921 tagtcatgct ggtttttaag ttcattttaa atccttacct attctcccta actgtgctat
    13981 acttaattta tactcaatga aaatcaaaga gcaaactaga aagctagccg caggctgttc
    14041 aaagcactgc tttgaggttg cagataaagt tgacgcagtt tgaagagatt ttcgaagagt
    14101 attagtacat tctttgagat tggagctagt atgaaaatcc ataaaaccgt gaatcctgtt
    14161 gcctatgaaa atacctatta tctagaaggc gaaaagcacc tcatcgtcgt cgatcctggt
    14221 agtcattggg aagccattcg tcagacaatc gagaagatca acaaaccgat ctgtgctatt
    14281 ctcttgaccc acgcccatta tgaccatatc atgagtctgg acttggttcg cgagacgttt
    14341 ggcaatcctc ctgtctatat cgcagagagc gaagccagct ggctctacac tcctgtcgat
    14401 aatctctccg gtctccctcg ccacgatgat atggcagatg tggtcacaaa acctgcagaa
    14461 cacacctttg tctttcacga agaataccaa ctagaggaat ttcgttttaa ggttctaccg
    14521 accccagggc actctatcgg tggtgtttcc ctagtctttc ctgatgctca cctagtcttg
    14581 acgggagatg ctctattccg cgaaactatc ggacggaccg accttccgac tggtagcatg
    14641 gagcaactcc ttcatagtat ccagacccaa ctcttcaccc taccaaacta cgatgtctat
    14701 ccaggacatg gtccagctac tactatcgct cacgaaaagg ccttcaatcc ctttttctag
    14761 caagatgatg acaatcgaaa tttaagtaaa ctatccagca aatctttcta ttacaaaagg
    14821 catcctatca aggttttcac acgtgattgg atgccttttt tctgatgact agattttttg
    14881 tattaccaaa taatcacgcg ctcctctggt gaacgccaca ttccgtctcc ttctttgaca
    14941 tcataggttg taaagaaatc gtcgaagttt ggtacttgca cattgacacg gagtttggct
    15001 ggtgcgtgca catcgacgct agccaaaagt ttcataaatt ctggtcgacc tttcatgcgc
    15061 cagatgcgac cgaagttgta gaagaactct tctgctgaga agtctgcttc tctcttagct
    15121 gcttcaagcg ctgctgcgat tcctcccaag tcagccacgt tttctgatac agtcaattta
    15181 ccgttaatgg ttgctccata agaatcctgt ccatcaaatt ggtcaatgac tttttgtgtt
    15241 ttctccttga aggcagcata gtcgctctct gtccaccaat ccttgaggct accattttcg
    15301 tcaaaggaag ccccgttagt atcaaaggcg tgggaaattt catgggcaat cactgcccca
    15361 ataccaccgt agttagcaga agatgactga tgcaagtcat agaaaggcgc ctgtaaaatg
    15421 gccgctggaa agacaatcag gttcttctga ggattgtagt aggcattgac catatgagca
    15481 ggcatgcccc attccttata gtctacaggc tggttccact tactccaact gtgcttgatt
    15541 tccacacgcg caaaggctag agcattctca aaaagactgg cagtttcatt cactacctta
    15601 tccttgtaac gtgcaggcaa ttcttctgga tagccaatat aaggtttgat cacattgagc
    15661 ttcacgatag cctgtttaca ggtttctgga gtgagccagt cattcttaag cagacgctcc
    15721 ttatagacat caatcatggt tgccactttt ttctccacat ccgccttggc ttctggagag
    15781 aacttctcac gggcgtacca aagccccagg gcttgcttga aaggttcctg tgctagatga
    15841 taagctgctt tgaccttatc ttttgcctct ggaactccag aaagggcacg gctgtaggca
    15901 ccagacaaaa cacggatatc ctctgttaaa tagctggttg aaagattgac aacactcaaa
    15961 atcaaggttg ctttaaggag agaccaggct tcctcactgt aaaattgctc tgctgcttgc
    16021 cagaaacgtt cctcgtctac aataaccttg tctggtaatt gcccaataac tgctttgaag
    16081 aagtcatcca aaggtagggc aggcgcgaat ttcttgaaat cttcgtaaga atatggatga
    16141 tagagtttag catattctga actttcttca ttagagagca ccactgccgc aactcggcgg
    16201 tccaattcaa gtcttttttc tagcaagtct tcaatttctt catcagagaa atcataagcc
    16261 ttgaggagat ttgcgctgct ttctttccaa agagtcaaga gctcttcgcg ctgaggatgt
    16321 tcttctgcat agtaggtcgt atctggcaag attgtgcttg gagcgctagc ccatagaaca
    16381 ttgattctag catccataaa gtctggcgat acaccaaaag gaaggaagtt tggttttcct
    16441 gcaagctcaa actctgctag tttagctgta aaatccgcaa aagtttccaa ttcttggaat
    16501 tctttaagga gtggtaagac aggtgtgata ccgtcagctt ctctcttgtc aaaatcacga
    16561 actaggcggt ggtatttgac aaagttttcc aagatagcat cctcaggcac ttcttcacct
    16621 gctaaccact tgtctgttgt cgccagcatc aggtcttcaa tttcctggtc taaatcaaca
    16681 aaacctcctg tttgagactt atctgctggg atttcagctg tctgttgcca ttctccattg
    16741 atagcatcat aaaaatcatc ttgataacgt gtcatcttgt tctcgctttc atttgtattt
    16801 gcatttatct taacaaaaat cactagggaa tgcaattaat aaccatttaa ctattatttt
    16861 t
//
LOCUS       DAVLSL010000032        16787 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLSL010000032 DAVLSL010000000
VERSION     DAVLSL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.032"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(82..1716)
                     /locus_tag="VXN30_000831"
     CDS             complement(82..1716)
                     /locus_tag="VXN30_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357487.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEMPIYFSSMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1697..2446)
                     /locus_tag="VXN30_000832"
     CDS             complement(1697..2446)
                     /locus_tag="VXN30_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357488.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            2625..4079
                     /locus_tag="VXN30_000833"
     CDS             2625..4079
                     /locus_tag="VXN30_000833"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1357489.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELRPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4169..6787)
                     /gene="alaS"
                     /locus_tag="VXN30_000834"
     CDS             complement(4169..6787)
                     /gene="alaS"
                     /locus_tag="VXN30_000834"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1357490.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAAILKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6809..7294)
                     /locus_tag="VXN30_000835"
     CDS             complement(6809..7294)
                     /locus_tag="VXN30_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1357491.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7569..7808)
                     /locus_tag="VXN30_000836"
     CDS             complement(7569..7808)
                     /locus_tag="VXN30_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357492.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8069..9139)
                     /locus_tag="VXN30_000837"
     CDS             complement(8069..9139)
                     /locus_tag="VXN30_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1357493.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9136..9909)
                     /locus_tag="VXN30_000838"
     CDS             complement(9136..9909)
                     /locus_tag="VXN30_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1357494.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9906..10712)
                     /locus_tag="VXN30_000839"
     CDS             complement(9906..10712)
                     /locus_tag="VXN30_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1357495.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10693..11850)
                     /locus_tag="VXN30_000840"
     CDS             complement(10693..11850)
                     /locus_tag="VXN30_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357496.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12010..12915)
                     /gene="murB"
                     /locus_tag="VXN30_000841"
     CDS             complement(12010..12915)
                     /gene="murB"
                     /locus_tag="VXN30_000841"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1357497.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13027..13779)
                     /locus_tag="VXN30_000842"
     CDS             complement(13027..13779)
                     /locus_tag="VXN30_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1357498.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13789..14520)
                     /gene="budA"
                     /locus_tag="VXN30_000843"
     CDS             complement(13789..14520)
                     /gene="budA"
                     /locus_tag="VXN30_000843"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1357499.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14662..15933
                     /locus_tag="VXN30_000844"
     CDS             14662..15933
                     /locus_tag="VXN30_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1357500.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15953..16768)
                     /locus_tag="VXN30_000845"
     CDS             complement(15953..16768)
                     /locus_tag="VXN30_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1357501.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
BASE COUNT         5031 a         3808 c         3331 g         4617 t
ORIGIN      
        1 tcttaatcat ttacaactac attctaacaa actataaaag cgtttgtcga attgaattta
       61 tcaagcaagc aaccaactat ctcatctttt ttctatttct gccaatatgc gtgacaggta
      121 ataatgatag ccaaaaatag caagagcaag caagacgata agagctccta ctcccaagtt
      181 gatggcaagg ataggggaga gagactgaac caagaatatg ctcccaatta caagggccat
      241 caggattgca ctataaataa acaataaaac tatggcgact atgccatttg aacgattcac
      301 caggtccgta atgctactcc aattggttga cagattttta acgtccttaa agtaatggtg
      361 gcaagaaagg atgacactgg caatgatcca gactacaaga aggtaaatca tcgaaaggat
      421 ggacaagcct agatatagag aaagaccgag caaagtcaga actggtaaaa aggactgaac
      481 agcaaagata atccaaaatt tcactttcac ataacgagca aagtcaaagg gtaaactctt
      541 cagaaaatca acattttccc tctccaagga caaggcaatt gaatgcaggc tggtgatatt
      601 gttattgaca actgctataa acactcctac aaaaaacaag ggtaaccagt atggaggatg
      661 aatgtctgga actatctgag aatctcggat tttggaaatc agactgatca tcatgagata
      721 aggaaggaaa gcacttgtaa aaagcactgt aatcacacca gtcccctgtc ccaagagggt
      781 gaggtggtag cgtaaaacca tacggaaaaa gcccttttta gtagttgaaa tcctctcctt
      841 gctgcgacgt tcttttttga ccttctcctc actgttaagc aggatcacgt cataaaaacg
      901 aggaaggacc ttctttttgg tcagataaag caggaagaga gttagtccta tccaagcgag
      961 cagacccact aaggcttctg tcgaaaaagg ctccactgct attttgtaaa agatatgaag
     1021 aggataaagg agaaatggaa tgtctctaac tttgtcaaca atacttccaa aagtcgactg
     1081 aagaaagaag ataaatatta aaggtatgag aactcctatc ccaatcatca cattcgaaaa
     1141 aatagactga tactttctga agaccctagt ctgagccaag aaatgtactg ccactaccgt
     1201 cactaaagta acagagacaa ataataaggt caaggacagt agcatcaaag gcaaacccag
     1261 ccaaagagaa ggagctagac taatatagag ggctagaaaa taagctagga ttggtacaat
     1321 tccagttaga gctggcaaga ggacagacag acctttggcc attataatct ctgattcttt
     1381 aaaggcatag ggcctatagg aggccaggtc cttactctca taaaagacat tgtaaaaggc
     1441 agtgaaagaa gttgaaaagg ccagcaccag taaaatagca accatcgagc taaaataaat
     1501 aggcatttcc tcaaaaggaa aatgaatggc tatattacta aaacagatga gcatcaagag
     1561 actggaaaaa atgtaagaac ttaggactct agcagaaaca tttacttttt tcccaggatt
     1621 cttagcctgc ttctttcgta gattagccag attggcttct tgagatgaat agaggatatt
     1681 gatatcaact aattttttaa tgaccttgag acgcatccgc aacctcctct tttctaccag
     1741 caagactaag gtagatactt tccaaaaact ggtctgggtg gtctttcctc aagttctcta
     1801 cactaccaca atagatcaaa tgcccctttt tcaaaatagc aatccgatca cagacttgct
     1861 cggctacctc aaggacatga gttgaaaaca agaccgtctt gcctttttgt gcatgttcct
     1921 tcatcatttg cttcaaatca aaggcggcct ggggatccaa accagtcaag ggttcgtcca
     1981 aaacccaaat atcaggagca gacaagagtg ccccaatgac aaagactttc tgacgcattc
     2041 cgtgagaaag agtttcaata acctgatagc ggttttcagc aaaatcaaaa acgttcaata
     2101 gcctagctag actagtctcc aagtcagagc tagtcagatc ataggatgaa gcgatcaatt
     2161 cccaaaattc attggccgtt aagcgtaaaa ataagtcagg cgagtctgct acgtagccaa
     2221 tctttcgttt gatcgccaag cgattttccg ataactcctg accgtctacc aaaatacgac
     2281 cactgctggg tgaaatgata ctgactaggg attttatggt ggtcgatttt ccagccccat
     2341 tatgaccaat caagcccata atctctccat tttcaatctg caaattgaga ttactcaagg
     2401 cctctttatc accatacaac ttactaacat tttgaaattc aatcatggga caactcctat
     2461 ctttatctgt attatacatc gctatactag cactttgata caaaaaaatc aatactttat
     2521 tttaagtagt taaaatagtt tccttctatc ttacgcaaac gtttgcgccg agagatactt
     2581 tatgatagaa taaagaacaa gattgacaag taagaggaaa catcatgcaa aatcaaacac
     2641 tcatgcaata ctttgaatgg tatctgcccc acgacggtca acactggacg cgtctggctg
     2701 aaaatgctcc acacctagct catctgggga tcagtcacgt ctggatgcca ccagccttca
     2761 aggcaaccaa cgaaaaagat gtcggctatg gggtctatga cttatttgac ttaggagagt
     2821 tcaaccaaaa agggactgtc cgcaccaagt atggtttcaa agaagactat cttcaagcca
     2881 ttcaagccct taaagaacag ggaattcaac ctatggccga tgtagttctc aaccacaagg
     2941 ctgctgccga taacagggaa gcctttcagg ttatcgaagt tgatcctgta gaccgtacag
     3001 ttgaacttgg agaacccttc accatcaatg gctggactag ttttaccttc gatggtcgcc
     3061 aagataccta taatggcttc cactggcatt ggtaccactt caccggtaca gactacgatg
     3121 ccaaacgcag taaatctggg atttatctga tccaagggga caacaagggc tgggctaacg
     3181 aggaattggt cgataacgaa aacggaaact acgactacct catgtatgcc gacctagact
     3241 ttaaacatcc tgaagtcatc caaaacatct atgactgggc tgattggttc atggaaacga
     3301 ctggtgtagc tggtttccgt ttggatgccg ttaagcatat tgactctttc tttatgcgca
     3361 acttcatccg cgatatgaag gaaaaatacg gtgacgattt ctatgttttt ggtgaatttt
     3421 ggaacccaga caaggaagcc aatctggact atctcgaaaa aacggaagaa cactttgacc
     3481 ttgtcgatgt tcgtctccac cagaatctct ttgaagccag tcaagctggc gcaaactatg
     3541 accttcgtgg cattttcaca gatagcctgg ttgaactcag gcctgacaag gctgtgactt
     3601 ttgtcgacaa ccacgatacc caacgaggac aagcccttga gtctaccgtt gaagaatggt
     3661 tcaagccagc agcctatgcc ctcattttgt tacgccaaga cggccttcca tgtgtctttt
     3721 acggagacta ctatgggatt tcaggccagt atgctcaaga agatttcaaa gaaatccttg
     3781 accgcctcct agccatccga aaagacttgg cctatggaga acaaaatgac tactttgacc
     3841 atgctaactg tatcggttgg gtacgttcag gtgctgaaaa tcaatcccca atcgcagtcc
     3901 ttatctcaaa tgaccaagaa aacagcaagt caatgtttgt cggtcaagaa tggactaatc
     3961 aaacctttgt agatttactt ggtaaccacc aaggtcaagt tacaattgat gaggaaggtt
     4021 atggacaatt ccctgtctca gctagatccg taagtgtctg ggcagtcaat accatctaat
     4081 agctcataat aaccaagcta ggtccaagcg gatttggctt ttttgtattc acaaaaagac
     4141 ctacccaaat ggatagatct ttacttgatt acaatttacc tgctactgca tccaacaatt
     4201 cttggatctt agcttggttg cttcctcctg ccatggccat gtctggttta ccaccaccac
     4261 gtccatcgat gattggtgct aattctttga caaggtttcc tgcatgaagg tcttttgtct
     4321 tgcttgctac aaggacattg actttgtcac cgatagcggc aactaggaca agaagatcag
     4381 agtagtcttt ttgtttccag ttatctgcaa aagtacgaag ggcaccggca tcggatacag
     4441 acacttgact agcaatgtaa cgatgaccgt tgacttcctt agcatctttg aagatatcgc
     4501 ctgcggctgc agctgcggct ttttctttca actcagcatt ttctttttga agttgacgaa
     4561 gttgttcttg aagtccttct accttgtgag gtacttcctt gacttgaggt gctttcaaga
     4621 ttgctgcgac agctttaaga gcatcctctt gttcacgata ggcttcaaag gcttccttac
     4681 cagtcactgc caagatacgg cgagttcctg aaccgattcc ttcttctttg acaattttga
     4741 agagaccaat ctcagaagtg ttgccaacat gagtaccacc acaaagttca atagagtagt
     4801 caccgatagt cacgacacga acttccttgc cgtatttctc accaaagagg gccatagctc
     4861 ccatttcttt agcagtgtca atatccgttt caactgtctt aacttcaaga gcttcccaga
     4921 ttttttcgtt gacttgctgt tcaatcgcac gcaattcttc agcagttaca gcttggaagt
     4981 gggtaaagtc aaagcgaagg aattcaactt cgttaagaga tcctgcctgt gttgcgtggt
     5041 ttccaaggat attgtgaagg gcagcgtgaa gcaaatgagt cgcagtgtgg tttttcatga
     5101 cacggtgacg gcgattgcta tcaattgcca aggtatattc ttggttcaag gcaagcggtg
     5161 caaggacttc aactgtatga agggcttgac catttggggc tttctgaaca ttggtcacag
     5221 tagccacaac cttacctgac tcatccaaga tttgtccgta gtcagctacc tgtccaccca
     5281 tttcagcata aaatgacgtt tccgcaaaga taagagaggc agttccttct gaaacagctt
     5341 ctacttctgc attgtccgcc acgatagcta ccaatttaga agacaattgg ctagcattgt
     5401 agttgaagac actttctaca gtgatgtttt gaagagtttc attttgcata cccattgagc
     5461 cacccttgac agctgaggca cgcgcgcgtt cttgctgttc tttcatggct gcttcaaaac
     5521 cttcacggtc tacagtcata ccagcttctt cagcgatttc ttcagtcaat tcaactggga
     5581 acccataagt atcatagagt ttgaagacat ctgaaccagc gataacagat tgaccttttt
     5641 ctttcaagtc tgctacaatg ccttgggcaa agtgttgacc tgagtgaagg gtacgggcaa
     5701 atgattcttc ttcgctctta acgattttct caataaagtc acgtttctca agcacttctg
     5761 ggtagtagct ttccatgatt tttccaacag ttggaaccaa tttgtaaagg aaaggttcgt
     5821 tgatacccaa tttttgacca tgcatagaag cacgacggag aagacgacga agaacataac
     5881 cacgaccttc atttcctgga agggcaccat caccgatagc aaatgaaaga gaacgaatgt
     5941 ggtctgcgat aaccttgaag ctcatgttgt cgccatcttg gtcataaacc ttaccagaca
     6001 atttctcgac ttcacggata atcggcatga agaggtccgt ttcaaagttg gtcttagccc
     6061 cttggataac agccaccaaa cgctccaaac cagcgcccgt atcaatgttc ttatgtggca
     6121 attccttgta ttcgctacga ggaacagcag ggtctgcgtt aaattgtgac aaaacgatgt
     6181 tccagatttc aatataacgg tcgttttcaa tatcttctgc aagcaggcga agaccgatat
     6241 tttctgggtc aaaggcttcc ccacggtcaa agaagatttc tgtatctggt ccagaaggtc
     6301 ccgcaccgat ttcccagaag ttgtcctcaa ttggaatcaa gtgacttgga tccactccca
     6361 cttcaatcca gcggttgtaa gaatctttat cgtctggata gtaggtcatg taaagttttt
     6421 cagcagggaa atcaaaccat tcagggcttg tcaaaagctc ataagcccaa gtgatagctt
     6481 cgtcacggaa gtaatccccg atagagaagt tccccagcat ttcaaacatg gtatggtgac
     6541 gcgcagtctt ccctacgttt tcgatgtcgt tggtacggat agccttttgg gcattggtaa
     6601 tacgtggatt ttcagggata atggtcccgt caaagtattt cttaagggtt gctaccccag
     6661 agttgatcca caaaagagtt gggtcattta caggaaccaa acttactgat ggttctactg
     6721 agtgagcttt ggtcgcccag aaatcaagcc acatttggcg tacttgtgca ctagatagtt
     6781 gtttcatatt gtctccttat ttacttgttt aatgtgattg gctttccagc atttccacat
     6841 agtcaatcgc gacacagagg gaaatgacta ggtctgcata agcgtcctca agaaccgtta
     6901 cggtataggt agaggtcaga tggaagagtt ccttcttaat ttccgcaatc agctgatcgc
     6961 gatcatccag caatttgaaa ttcaaatccc agatattgcc ctcgatacga agacctagat
     7021 tatcaaactc atacttatct cgccagaagg tcaacttctt acgaatgaca aaactcgagc
     7081 catcccgaag ctgaatctca aaacgaggaa gcaaggtcaa gatttcttta ctaatctcac
     7141 tgacttgttc accagccgca tcatagatgg taaaggtttt aggaatctta aaaaatgatc
     7201 cctccacctg ataggcaatt tctcccctgt cattcttgat agcgaagcgt tcgcctccaa
     7261 gacgaaactt ttgtttgaca agaaatgttt tcatcaacac ctccaaaaat caaaagacaa
     7321 gctcatatca cgaagggcga aaaaccgcgg taccaccttc attcaatgaa cttgtcattc
     7381 tcttgttctt atgcaattgt atgattgagt agcatgactt cctagcttag atggctcgca
     7441 gcaccgccat ttctctggac taagacaagt gaaaatcaat tctcaacttt cttattataa
     7501 cgttttttta agcttgcgtc aactggaaat gatctccgtt gaattagacc aattccctac
     7561 atctctgatt actttttcag gatatatttt ttcttactgc cgtttttctt tttatcccaa
     7621 attttcatat tactaaacac agctactaga atatttccaa atataaaggt gcctatcacc
     7681 caatatatgg actcagttgt taggtattgt cgatccaagc catcctttaa atggaatagt
     7741 ataacagttt ggttaacaat cataaaggtt ggccagaaac tttttttgaa aaaagtagac
     7801 attttcatta tttgttgccg ctttctgtaa ggttaatact caataaaaat caaaaagcaa
     7861 actaggaagc tagcctcaag ctgtacttga gtacggcaag gcaacgctga cgtggtttga
     7921 agagtatagg cttagtatac tactaggcaa gcaaataagc aaataaacaa ataaacaact
     7981 agaatagaaa aagatagggc tctaaaaact gacttctatt ccttaaaaac gaaccagctt
     8041 gactgattcg tcttcttacg tttatctcct acttccgata cattttaaac tgtaggaaga
     8101 ggtcgctata tttccctgtc catttatggt caaatttctc ataaacttct aggtgtttca
     8161 tggtttcaac atcgggatag aaggccttat cttcctttgt ttcctctggg agcaattcct
     8221 tcgctggtag gtttggtgtt gaatagccga catactccgc attttggaga gcattttcag
     8281 gtttcaacat aaagttgata aaggcatagg ctgagttttg gtttttaact gttttgggaa
     8341 tgaccatatt gtcaaaccaa agattgctgg cctctgtcgg taccacataa cgtagatttt
     8401 catttttttc taacatttgg ctggcttcac cagagaaggt cacgccgatt gcaacattat
     8461 tctgaatcat atagcccttc atctcgtccg caacgatagc cttgatattt ggagtcagtt
     8521 tgtagagctt atccactgtc tcttccaact gctgcagatc cttggagttg aggctgtagc
     8581 cgagggaatt gagtcctagt cccagcacct cacgcgcccc atcaaagagc atgatagaat
     8641 tcttatactc cggcttccaa aggtcatccc aatgctcagg cgcttcatct accatggttt
     8701 cgttgtagac aattcctaag gttccccaga agtaagggat ggagaattta ttacctgggt
     8761 caaaggactg gttgagaaac tctggtccga tattttcgat tcctttaatt tttgaataat
     8821 caagcggaac caagaggtct tcgtccttca tcttgttaat catgtattca cttggaatgg
     8881 caatatcgta ggtcgttcca ccctgcttta tcttagtgta catggcttcg ttggagtcaa
     8941 aagtctcgta ctgaacttga attcctgttt cttctgtaaa ctgagtcaag agttcaggat
     9001 cgatatagtc tccccagtta tagataacca atttttgact atctcgacta ttgattttac
     9061 tatctaaatg agtcgcaatt ccccacaaga caaggataat cgctgcaatt cctgctaaaa
     9121 atgaatagat ttttttcatg cttgctcctc cttctcacga gagataaagt aataacctac
     9181 aactaggata atactaaaga gaaagactag agcagacagg gcattgattt ctaaggaaat
     9241 ccccttgcga gcacgagagt aaatctcgac tgatagggtt gaaaagccat ttcctgttac
     9301 aaagaaggtc acggcaaaat catccagcga ataggtgaag gccatgaagt aacctgcaat
     9361 gatagacgga gtcaggtaag gaagcatgat ttccttaaac atctgaaatt gactagctcc
     9421 caagtcatag gccgcatgaa tcatgtcgcc attcatttcc ttgagtcgag gcaagaccat
     9481 caagaccacg ataggaatgg agaaggccac gtgactagat agaacggtca aaaagccaag
     9541 tgaaaacttg agttgggtaa agagaatcaa gaagctagca ccaatcataa cgtcaggcgc
     9601 aaccatgagg atattattga gtgatagaaa ggcttcttgg tatttcttac gagactggta
     9661 gatgtaaatg gcaccaaaag tcccgataat ggtcgctatc aaggctgata ggaaggccaa
     9721 gaaaaatgtc tgagccaaaa tcagcatgag tctcccatct ccaaacatgg tttcaaagtg
     9781 agtccagcta aaacctgtaa agctattcat atcatcacca gcattaaagg catagccaat
     9841 caagtaaaaa atcggcaggt agaggaccag aaagaccagt cccagataaa ggttggcaaa
     9901 ttttttcatc gttctctcct ttccttggtc acccacatgg tgatgaacat ggtcaggatg
     9961 agaatcacac cgatggttga acccatacca tagttgtcat tggttagaaa attctgctca
    10021 atagccgtcc ccaaggtgat aacgcggttc ccaccaatca aacgggtcag catgaagaga
    10081 ctcaaacttg ggataaagac cgactgaacc ccacttcgca caccgttcat agatagaggg
    10141 aagatgacat gacggaaggt ctcccactta gttgcaccaa ggtcgtaact agcattgatg
    10201 agattattat ccatatcgtc taagacattg aaaatcggca aaatcataaa ggggagctcg
    10261 atgtagcttg cgacaaagat aaaggaaaaa tcggtaaaaa gcaactgttg tgaaccaatt
    10321 ccgataaatt ccaagaattg gttaatagag ccattttgac caaaaatccc gataaaagca
    10381 taggctttaa ggagcaaatt gatccaggta ggaaggataa tcagcatgag ccagagttga
    10441 cggtgcttga gacgggtcaa aaagagggct gttggatagc tgataagcaa ggtcacaaag
    10501 gtcacaatcc ctgcataaag cactgagttg aaactcattt tgaggtaggt caagttttgt
    10561 gacgcaaagt aagatttata attttctaaa ctaaactggc cttcaatgtt gaaaaaggat
    10621 tgtccgaaaa tcaagaccaa gggtgccaat acaaagaggg caatccaaag catgtagggc
    10681 actacaaaga gtttagagct tgttttcttc atctctttcc tcctcgatcg cattgatcag
    10741 acctgcttct tgctcttcga tttccacgta ttcttcgata cgagcatcga actcttcttc
    10801 ggtttcattg agacgcatga tgtggatgtc ttctgattca aagtccagac cgatttcctc
    10861 acccacgatg gccttacggg ttgagtggat catccattca tttccaagtt cgtcataggc
    10921 gataatttca taatgaactc cacggaagag ctgggtgtcg accttaactt ggagtttgcc
    10981 ttcttcagga agggtaatgc gcaagtcctc tggacgaata acgacctcaa caggttcatt
    11041 tggcttcatc ccaccatcaa ccgcttcaaa gcgtttgccg ttaaattcga ccaagtagtc
    11101 ctcaatcatg gtacctggca agatgtttga ctccccgata aaggtggcaa caaagtggtt
    11161 gattggctca tcgtagatgt ccacaggggt tccagactgg acaatctcgc catcattcat
    11221 aacgaaaatc cagtcactca tggcaagagc ttcttcctga tcgtgagtga caaagacaaa
    11281 ggtaatgccc aatcgttgtt gtaattcacg caattcgtac tgcatgtctg ttctcaattt
    11341 caagtccagc gctgataaag gctcgtccaa caagaccaca cggggttggt tgatgatagc
    11401 acgggcgatg gccacacgct gacgttgtcc tccagaaagt ttgcggatgg aacgtttttc
    11461 ataaccttcc aactgaacca tcttgagaac ttccgctaca cgctgctcga tttctttctt
    11521 atcaatttta cgcaagcgaa gtggaaaggc aacattttca aacacattca tatgtgggaa
    11581 caaggcatag gattggaaga cggtatgtac gtcgcgcttg ttggttggaa tatcattgat
    11641 acgaacaccg tctagcatga tatctcctgt cgtcgcatcc agtaaacctg caataatgtt
    11701 taggatagtt gatttccccg aaccagatgc acctagaagg gtgtagaatt tcccttcttc
    11761 caactcaaag ttgatgtctt tgagaacctt ggtgttgctg tcttcaaaaa ctttagagac
    11821 gtttttgaat tcgataattg gttttttcaa ttggcataaa ttccttcttt ttcatagatt
    11881 aaccgatcgg ggctctgtca ggtccccact acctcttgca gggagtaaaa ccacctgcat
    11941 acatcttcgc taccgatagg ctttcacccc ctttccatga catagcagca gcttttcaaa
    12001 tacagcttcc tacttgcttt cacccaagat ccggacttct ctttcaagcg taatacctga
    12061 gtgttccttg actttttcga taaccgattg gatcaagtcc tcgtagtctt tggccgttcc
    12121 atctgcgaca ttgatcataa atcctgcatg cttttctgac acttctacgc caccgatacg
    12181 atagcctttc aagccagctt ctgaaattaa ctgacctgca aaatgcccga ctggacgctt
    12241 aaagaccgag ccacaagatg ggtattccaa aggttgcttg agttcacgta ggtgcgtcaa
    12301 gcggtccatt tcctgcttga taacctgatg ggttcctggg gctagggcaa atttaactga
    12361 caagacaact gcaccagact cctgaatagc tgaatggcgg taaccaaaag ccaagtcttt
    12421 agcagacagg gtttcgattt ctccatcctt ggtcaagacc ttacaagact gcaagatgtg
    12481 agcaatctcg ccaccatagg cacccgcatt cataaagaca gcaccgccaa cgcttcctgg
    12541 aataccacaa gcaaactcaa agccagtcaa actatgacgg agggcaatgc gagttgtttc
    12601 aatcaagtta gccccagctt ctgcttcaat ggtatagcca tcaacagaaa cgttattgag
    12661 cttgtcacac aagatgacaa atccacgaat cccaccatca cgaacgatga tattgcttgc
    12721 attgccaaga accatccaag ggatattttc ttggttggca aatttcacaa ggcgcgccaa
    12781 ctcaaaacga tttcgtggaa agaccaaata atcagcctct ccacctactt ttgtataact
    12841 atagctatgc aagggttcct taaaacggat atcaattcct tctaagattt caagcatttt
    12901 ttctcttaca gacatgtcac tcttcctttt acaaaattca ttccattata ccatttttag
    12961 agacatttga cgaccataaa aataccttgt ttggattttg cataagaaaa agaggttccc
    13021 ccctttttat gattttttac aaaagatttc cttggttcca taggcgacca gaacgagctc
    13081 cagtgctaga atcacttcaa ccaagactgg atttgtcaac cagcctactt ggaaaagaga
    13141 tggtgccaga tcaaagaagg catgcaagcc ataggctgct aggagataaa tccatttctt
    13201 ctggcgaaca gcttggtaaa cccaaactgt caaaagtaat tggaaaccaa gcgccaagat
    13261 tcgctcaaaa ccaagcaaat aaatctgcca gaccgaaagt gactgaatgg tttttaacat
    13321 attttcagac agtaattgca taacctgtgg attctgagtt tgaactgccg aaagaacgat
    13381 gtaaagattg agtaaactaa taaggcctag aaaaatcaac tccaagccac catgccctag
    13441 gccataggcc aaggcatctg ccttttccaa acttctcttt ttctccaacc atttgaagaa
    13501 aacaagacga gccgtttcct caaaaaatgc tgccatggct aggccataga taatatagat
    13561 aagcggatga tcttgcaaga gggcaatact accgtctttt tgagggtgca aaatcaagat
    13621 atgcaccagt ttttccaaaa tctgcgaaga gacaaagaaa gcgactgcac ccaagcccaa
    13681 gacagctaga ttaatctggt atttcttttt ggcaaaccaa atgcttccta tcagaaaagc
    13741 tagcaacagc aacatggtaa taatgatatg aatggtcatt ttcttctcct attctgcctt
    13801 ttcaatatct tttttcatct cgtcaacatt gaacttagca aacaagtatt gacggtcttg
    13861 gactgggaaa cgttggtcca actggtcaac tgctcccacc tcgataatgc cttccttgat
    13921 gacaaaatcc atgacatgtc caccgaaggt caaatcatct gatatgaagt gcagatggta
    13981 gcctgccaca ctgaccccat ggaaaatctc aggcgtccag aaaccaacaa tagtccccgc
    14041 aacattgtca cgactatatt ccggttgatg ggttgcgaca tcagcaaact tagtatcggg
    14101 tgttgactta ggaatcatac gcacatgcat atgtgaaaat tctccccgaa tcttgataga
    14161 acggaaaaga ttttccccat cataataaga ctcaattcgt tctttcaatt ccttgtctgt
    14221 catctcaaag cgctggcgga aaatgacctc tgcctgatgc ggtaccactg cagcgtaagg
    14281 aataagggca tctggtgaca cttccacaat ttctggctgg tctcctgacc ctttggcctg
    14341 ataagccttg ccatccaaga caatcaattc cccatcaata gaatccaagg ttcccaaacc
    14401 aaggtcacca tgctctagca attctcccac tgtcatggta ccaccataaa ggccagccat
    14461 caaggctcca agagtattgt attgaaataa tttcactggt tcctgcacgt tcttatccat
    14521 tcactttctt gtctgttatt ctataaatct tactcctaag tataccacat ttgcccctag
    14581 atgtgaacga gagaaacgct ctagacattg ccaagaagga aaaaaaaggg tacaatgtaa
    14641 caaaatcaag ggaggtctgg aatgaagaaa caaagcaagt acaaagaggt cgtttcctat
    14701 ctgaaaaatg gtatcgagtc tggacgattt ccgacgggta gtcgcctgcc ttctatccgt
    14761 caactgagcc ttgactttca ctgtagcaag gacaccattc aacgagccct gctggaatta
    14821 cggcacgaac aatacctcta tgccaagccc cagagtggct actatgtcct agaacaaggg
    14881 caacaccagg acctagaaat cgaggttacc gacgaacatg ccagtgccta tgacgatttc
    14941 cgactctgtg tcaatgaaac cttgattggc cgagaaaact acctcttcaa ctactatgat
    15001 aatcaagaag gattagaaga cctaagacaa tccattcaca aactcctctt tgagcaagct
    15061 ctctactgca aggctaacca actagtactg acttctggaa cccaacaagc cttgtttatc
    15121 ctctctcaaa tatcctttcc tagccaagcc aaggaaatct tggtggaaca gccaacctac
    15181 catcggatga atcgcctctt gattgcacag gggctggact atcaaacgat tgaacgaggc
    15241 attgatggga ttgacctgaa ggagctggag cgacacttca agacaggaaa aattaagttt
    15301 ttctacacca ttccccgatt tcactatcct ctgggacatt cctattctga gcaagacaaa
    15361 cgatccattc ttaacttagc tgccaagtat gatgtctata tcgtagaaga cgattatctg
    15421 ggtgatttgg actccaagaa gggccaaacc ttccactatc ttgatacaga ggagcatgtc
    15481 atttatatca agtccttctc gaccagcctt tttcctgccc ttcgtattac agcactcatt
    15541 cttccaaatg ctatcaaaga agcatttgtg gcctacaaaa atatcctaga ctacgacagc
    15601 aacctcatta tgcaaaaggc cctgtcactc tatattgaca gtcaattgtt tgaaaaaaat
    15661 cgtttggctc gcttgaccaa tcatgaatct taccaaaaac aaatcgagga aaggataact
    15721 aaaacacctt gtccccttcc tcattatccc ctacacgatg gtttattgct agacctgaga
    15781 cagtatccta aaatcgccag tctcaaacac agtcaaccgg gcttggactt ctttgaagag
    15841 gcctatttaa gtacctgtcc ttatcaattt gccaaggtgt ccctagacaa tctggaaaat
    15901 gttttaaact atttaaaagc agaattggaa tgacatccaa ctctgctttt tcttattctt
    15961 caacttcagt tagacttgct gctttttcaa gatagctttg gtaaattcct tcatccttaa
    16021 gtttttggat gaccttgtct accacttctt tcaaatcatc actatttttt ctaagagcaa
    16081 cggcattggc gtcgccgtcc ttcatcttca agctgacagt tgcgacagct aagccagcgt
    16141 ttttagcagc ataactaagt gcaacaggct catccatatg aacagcatct acttttccag
    16201 cctgcaattc attgactgct tcacccatat tagttaggga agttaattga gcttttggca
    16261 attgttcctt gaccattgat tctggaacag tccctttttg ggctgcaata ttagcacttt
    16321 ctaggctagt taaatccttg tatttttcca catcagcctt acgaaccaag aaactaatct
    16381 tgttttcata gtatgggatt gaaaaatcaa agacttcttt tctctcgtca gtagcactaa
    16441 ttcctgcaac tgctaggtca gccttaccag tttgaagact ggtcaaaaca ttgtcaaaac
    16501 tcatgcttga gatttccaac ttaaccccaa gttcatcagc gatagcctga gccatgtcga
    16561 tgtctgcacc gactacctgg ttctttccat caaccaatga ttgaaattca aagggtgcat
    16621 agtcaggact ggttgccaca actaatttcc ccttttgctt aatggcctca acagctgact
    16681 gagaaccatt agaacctgac tggcaagcta ctaagaagaa acttgcaaga aaattacata
    16741 aaacaaatat ccattttttt gatttcataa ataaacaacc tctctgt
//
LOCUS       DAVLSL010000033        16579 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLSL010000033 DAVLSL010000000
VERSION     DAVLSL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16579
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.033"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(134..697)
                     /locus_tag="VXN30_000846"
     CDS             complement(134..697)
                     /locus_tag="VXN30_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1357502.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(681..1232)
                     /gene="coaC"
                     /locus_tag="VXN30_000847"
     CDS             complement(681..1232)
                     /gene="coaC"
                     /locus_tag="VXN30_000847"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1357503.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(1244..1933)
                     /gene="coaB"
                     /locus_tag="VXN30_000848"
     CDS             complement(1244..1933)
                     /gene="coaB"
                     /locus_tag="VXN30_000848"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1357504.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2163..3833
                     /locus_tag="VXN30_000849"
     CDS             2163..3833
                     /locus_tag="VXN30_000849"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1357505.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            complement(4324..5490)
                     /locus_tag="VXN30_000850"
     CDS             complement(4324..5490)
                     /locus_tag="VXN30_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1357506.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(5503..6327)
                     /locus_tag="VXN30_000851"
     CDS             complement(5503..6327)
                     /locus_tag="VXN30_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1357507.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            complement(6462..7676)
                     /gene="thiI"
                     /locus_tag="VXN30_000852"
     CDS             complement(6462..7676)
                     /gene="thiI"
                     /locus_tag="VXN30_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1357508.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(7685..8827)
                     /locus_tag="VXN30_000853"
     CDS             complement(7685..8827)
                     /locus_tag="VXN30_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1357509.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8965..9393
                     /locus_tag="VXN30_000854"
     CDS             8965..9393
                     /locus_tag="VXN30_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357510.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            complement(9544..11769)
                     /locus_tag="VXN30_000855"
     CDS             complement(9544..11769)
                     /locus_tag="VXN30_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1357511.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(11933..13885)
                     /locus_tag="VXN30_000856"
     CDS             complement(11933..13885)
                     /locus_tag="VXN30_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1357512.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHSYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQVP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(13882..14793)
                     /gene="pfkB"
                     /locus_tag="VXN30_000857"
     CDS             complement(13882..14793)
                     /gene="pfkB"
                     /locus_tag="VXN30_000857"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1357513.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(14790..15530)
                     /locus_tag="VXN30_000858"
     CDS             complement(14790..15530)
                     /locus_tag="VXN30_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1357514.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            15692..15779
                     /locus_tag="VXN30_000859"
     tRNA            15692..15779
                     /locus_tag="VXN30_000859"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15726..15728,aa:Ser,seq:gga)
     gene            complement(15962..16309)
                     /gene="ssrA"
                     /locus_tag="VXN30_000860"
     tmRNA           complement(15962..16309)
                     /gene="ssrA"
                     /locus_tag="VXN30_000860"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         4861 a         3543 c         3302 g         4873 t
ORIGIN      
        1 gttagaaatc gatttggctg tcctgatcga tttgtcctgt tcttatttcg ttttactaat
       61 agtaaagatt tcattaaaaa gaaactgtat agagcaaaat ctccaccttc aggtttgaaa
      121 gcggagattg tttttatttt ttcaaggttt gtagtcgtgg aacaatggct agggttagaa
      181 ttgcagaaat gaccaattca gcaattgaat ttgttgagat aacggttgcc agaagaagtt
      241 ggatatttcc attataaaca tttccaaata ggaagaagat tcctccaagg acaaagatag
      301 tatttgtcaa ggaaccaagg gccccagcta aaatcagacc agtcttgttt ttcatcagtt
      361 tatagactaa gtaaggagtt aaaccaatca aaatacgtgg gacaatggca atgatagctg
      421 agtagatgtt tccgtttggt acgaagggag agaagaggta gcttgtcggt agaatcgtaa
      481 tcgtgttaac cgtcaagcta agtaatccca tcaaaaatcc aagtgtaacc ccaactcgtg
      541 gaccataaat aatgctggca ataatgacag gaatatgaac aatggtcggt ttgattggaa
      601 atggaaaaag gttaaagata agtgagctca gaaagtgtat cacgagcatg gtagcaaaaa
      661 agatagcaat gggtgcaata ttagagcgtt ttttcatcga tagtttcctt tattctttct
      721 aaaataattg tgaggtcagc taaagctcct cgtccgtggt ctccacaagc tagtagggat
      781 tccttaggag caatcagctg atagccgtaa gtttctaatg ttttcagatt attctgagtt
      841 actggatggt catacatttt tgtattcata gcaggagcta ttagtttggg aatatgactt
      901 ggtagggcta gagctgtact ggttaccatg ttgtccgcaa atccgtgagc tagttttgca
      961 atagtgttag cagttgcagg taccacgata aataaatctg ctttttttcc aagttcgata
     1021 tgattgacct gatcaggata gggttccttc atgacatcca agtggacagg attctgtgag
     1081 agtacctgta gtgtcaaagg ttggataaac tctgtagcag cctgagtcat taagacagtg
     1141 acttgatggc cttgtttttt tagagaactg actaaatctg ccgacttata agaggcgatt
     1201 gaacccgtta cagccaagag aatgtttgcc atagttttcc tttctaagaa tgataggctt
     1261 gaattttttc aaggaggagt tctgcaattt cttctttagt ctggactgtt tgaagctgat
     1321 ttttctcaac aaatatagct cggtgctgac ctgctgaaat ttgagtcagg tcattcgcga
     1381 tgattaaatc tgcttgattc ttgataagac tttttcgtgc aatgtcaacc agatgatctt
     1441 cggtaacatc aaccagcagt ttgaaaccaa tcagatgaat agtaggattc cattccttga
     1501 ctagggatat gattttgggt gtctttttaa ggaacaaaac ctgaacctca tcagttgaag
     1561 aaatcttggc ctgatgattc tgcttgctta aaaattcttt tagattggag ctagcctgaa
     1621 cttcctcaag ccctgtcata taaacaggag tgtagtcaga aacagccatt gagtggatca
     1681 agacctgata atcctgaaca cgttcttgca tttctattag aaggtccttg gtattggtaa
     1741 tttctcgaat acttaggtta ggatgaggct ctggcttcag agctcgtttt gtcgtaatta
     1801 aacaaacttc atgccctgca aaaagcaaag tctctgtgat aattttcccc aagtgacctg
     1861 tagaatggtt agtgatagag cggacgctat cgatagcttc actggtaccg cccgatgtaa
     1921 ctaaaatttt catagcacta ttgtacacaa aaataagcgg taagtaaaat gaaagtgata
     1981 aatttttggt aaaaacgaag atttattata gtttcaaact ataaagccat ataaaattta
     2041 agaaaggact ctaaaagtct taaaaacaga gatatttacg aacgtttaga gagttttagg
     2101 atgttaaaaa tcttgttttg tgatataatg aattatcata taagaggtta gaggaatttt
     2161 gaatgaaaac agatattgaa atcgcacaga gtattgagtt gaagccaatt gttgatgttg
     2221 tagagaaact tggtatttct tacgacgatt tggagttgta cggaaagtac aaggctaaac
     2281 tcagctttga taaaattcgt gcagttgaga gcaatccagt cggtaaattg atcttggtta
     2341 ctgccatcaa cccaacacct gcaggtgaag gaaagtcgac gcttaccatt ggtcttgcgg
     2401 atgccttgaa caagattggc aagaaaacca tgattgctat ccgcgaaccg tctcttggtc
     2461 cagtcatggg gatcaagggt ggtgctgctg gtggtgggta tgcacaagtt ctgccaatgg
     2521 aagacatcaa cctccacttt actggagata tgcatgctat tacaactgcc aacaatgccc
     2581 tttctgcctt gattgacaac cacttgcacc aagggaatga gctggaaatt gatcaacgtc
     2641 gtatcctctg gaaacgcgtt gtggacttga acgaccgtgc gctccgccat gtgactgttg
     2701 gtcttggtgg tcctctaaac ggtattccac gtgaggatgg ttttgatatt acagttgctt
     2761 cagaaatcat ggcaattctt tgcttggcaa cggacatcga ggacttgaaa cgtcgtttgg
     2821 cgaatatcgt tattggttat cgctatgacc gtacgcctgt ttctgtaggt gatttgcagg
     2881 ttgagggtgc cttggctttg attttgaagg atgctattaa gccaaacttg gttcagacaa
     2941 tttacggtac acctgccttt gtacatggtg gtccatttgc caatatcgct catggctgta
     3001 actctgtttt ggcgacgaca acagcccttc acttggctga ttacactgtt actgaagctg
     3061 gttttggtgc ggatcttggt gctgagaaat tccttgatat caagacacca aacttgccaa
     3121 catctccaga tgcagttgtt attgtcgcaa cccttcgtgc ccttaagatg aatggtggtg
     3181 tggctaaaga cgctctgact gaagaaaatg tagaggcagt tcgtgcaggt tttgctaact
     3241 tgaaacgcca cgttgaaaat atccgtaagt tcggtattcc agcagttgta gctattaacg
     3301 aatttgtatc tgatacagaa gctgaaattg cagtcttaaa agaactctgt gcctcaatcg
     3361 atgtaccagt tgaattggct agtgtctggg ctgatggagc agaaggtgga gtagcacttg
     3421 ccgaaacagt tgttaagaca attgctgaaa atccagctaa ctataaacgt ttgtacgata
     3481 atgacctttc tgtccaagaa aagattgaaa aaattgtcac tgaaatctat cgtggtagca
     3541 aagtgaactt tgagaagaaa gctcaaacac aaattgctca aatcgttcaa aacggttggg
     3601 acaaattgcc aatttgtatg gctaaaactc aatacagttt ctcagacaat ccaaatgcgc
     3661 ttggagcacc agaaaacttt gaaattacca ttcgtgaatt ggtaccaaaa ttaggtgcag
     3721 gcttcatcgt tgccttaact ggtgatgtta tgaccatgcc aggtcttcca aaacgtccag
     3781 cagctctcaa catggatgtt gaaagcgatg gaactgtact aggcttgttc tagtggattg
     3841 aaattgactt taagtgagtt tggaaataat atccaagctc attttttctc agactgcaag
     3901 cagttcatca ataagattta tactcttcga aaatcttttc aaactacgtc agcttcacct
     3961 tgccgtaggt atatgatact gactctgtca gttctatcca caacctcaaa acagtgtttt
     4021 gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta ttaatagact
     4081 ggtttggtat cctgaaaact atatcgtata ggtgtcgtca tggatccttt tagctgattt
     4141 aaggatacga aaacacaata ctatgactaa attgaaatgt taaaattcaa tttctagcca
     4201 cctaatcctg cttcaaatag ttatcgaaca agttttatga tagctacctt gttaggatgc
     4261 tcttattttt taccaaacaa aacaaaagga ttaagggagt gaatttcctt aatccgatag
     4321 ttcttattct tgacgttgtc cgaaatcagc aatcatctga tccatttctt gtcgactcgg
     4381 agttgtcagc atatacaggt aatctccttg aagttccgcg aaggcagctg gcatgacttt
     4441 tttaaccttg aactgctggc tatatttggc aagcaaatcc tcttctgaat aaacatagtg
     4501 agcatttgca cggcgagaag tagccaaaca atactgagtt tcaaagtctg acgccgggaa
     4561 ctcctctcct gcgacaatag ctgcatagcc acgataaagg tccaaggaat gagcaaagtt
     4621 ataaacatca atggtaaaac cacctgcagg gcggttattg tactcgatgg taatataatc
     4681 gtccccctca cggaagaact caatatggaa aaaccgttct ttcataccaa attctttgac
     4741 aattgcttcc ccatacttgc gcagtttagg atccatatcc ttgagcacat aataagaatt
     4801 gtccatctta taaatcatga ggtcaagcgg tgtataggcg tagtcaaagg ttgttgagaa
     4861 gacaatcttt ccatccttgt ccacgagccc gtcaaaggta cagatttcgc tggaagtgac
     4921 aaatttttca aagaaataaa gggttgaatg gtcccattct tgcttgaagt gattgatatc
     4981 gtcttctgtc tcaagtttaa aggttgcggc tgctcccact ccattatcag gtttggcaat
     5041 cattggaaga ccgatttctt tcactgcttg atcaacatct gcttccgtct tgataacagc
     5101 tccaggtacc acaggaacac ctgctttttt gaaaagtttc ttcatttcag acttatattt
     5161 cgtctttttg agatcctctg gtttggcacc aaaaacattg aattgttctc tgagtgttgc
     5221 gtctagctca agccagtatt cattgtgaga ctcgatgcgg tcaattggac catgtttata
     5281 aaagagaaaa gcaactgcac gtttgacttc atctatgttc tcaagattat caacacgaaa
     5341 atactcggtc aagctattgc gcaagggctc atccaattgc tcgtaagact cttgaccaat
     5401 tcccaagact gtgatgcctt tattagctag ttcgatggta aactgttgaa agttttgtgg
     5461 atagtaggga gaaataacaa ggtaattcat aggtaactcc ttttataaat agagattacc
     5521 gaggaaataa ggcatttgtt tacgccacca ttcccagtcg tgggcgacat catgtcccca
     5581 ttcagcaaac caggctggaa tttgtttctg gtcaaaggct tctttgagct tgtaaaagga
     5641 tggtaaacca tcttgttccc aggctccaag ccccgtacac agcacaatct ctgcctgacg
     5701 gtaacggtca ataaaccagc catcgttttg gttccaaata taatctactg gcgagttttg
     5761 gtaaatagca tcatcgttgt agtaatcgcc gacaaagaaa cgtgcgtcgt aaacaccact
     5821 gagagcaatc actttggtaa agacatctgg atgctggagg aagaaattga gtgcatgata
     5881 ggctcccata gagcaacctg tcgtcatcat gccatcaaac caacctgttt tgtgcttgat
     5941 aaaaggcttg accacctcca tgacaaactc agcatactcc acacccttac caccaaactg
     6001 ctgctctggg ataggagatt cttggaactt ccaagccgca tactcattca tccgccccat
     6061 accatcattg tcaatagcaa cgacaatcat gcgactgata tccggatttc gtttgatagc
     6121 tgggataatc ttccatgaat gtccaatgaa agactctttg ctattaaaaa cattttgccc
     6181 gtcatgaaag tatacaacag gataggaacg gtctgtatct ttctcataat ctttaggaag
     6241 aagaatacgt acacggcgct ccttacctgt ataaggaacc ttgagtttgt gttctttcat
     6301 ttttagataa aagtaggatt gattcattgt cagaaaactc tctatattca aaattttata
     6361 tcattatatc ataaaaataa aaaaagttag ttttttacct gtttttagag aaaaaactaa
     6421 ctttttttac tgattttcgc tattcttttg gattttctga attagagcag attgtcaatc
     6481 aagtcatcaa cttcatcttt ttcggcttga ggtgtgattt cagtaatcat gattccagcc
     6541 actgctcgct caaccaagcc ttcaacatcc atacgcgctt cgtactgctc cgcattctta
     6601 attttaggat ttgtttttgg acgatctggt gcaaaaatag tacaacagtc ttcaaacggt
     6661 tggattgaaa tgtcaaaggt atcgatttcc tgggcgatgt caatgatttc caacttgtcc
     6721 atggtaacca caggacgaat gatgggagtg ttggtaacag cattgatagc cttcatactt
     6781 tcaagggttt ggctggctac ttgacctaga ctttccccat tgatgataac taaaccattt
     6841 cgtacctcac gaatacggtc agtaatacgc atcataaaac gacgcgtcaa ggtcataagg
     6901 taggcttctg gcgctttggc tttgatttcc tcttgaatct ctgtaaaagg aacttcgata
     6961 aactggatat ttcccccaaa tttggttaat ttacgggtca aatcttgggc ttttttaaga
     7021 gcaccaggac tcgtgtaagg tgggctggca aagtgaactg cctctatatc tacccctcgt
     7081 ttaagcgcta gatagccagc tacaggggaa tcgattcccc ctgacaacat gagcatgccc
     7141 ttaccagaag ttcccactgg taatccacct gcccctcgaa cggtttcata agaaagatag
     7201 gctgcttctt cacgtatctc cacctgaaga ttgatgtcag gacttttcat ttgagcttgt
     7261 acgtttggaa tggcttcgaa tacagcccct ccaagcgttt ggttgagttc acgactgtcc
     7321 aattcaaagg tgtggtcgct acgcttgcta gagattttaa aggtcatgcc ttccttgtag
     7381 atgtcctgca taatctcttg gacagcagac ttcagaactt ctacagattt ttcaacctta
     7441 taaacagggg aaaagttttg aattccaaat acttgtttga gcgattctgc tactgctgtg
     7501 taatctgctc cattgaggta agcgtgggca cggtcgcgat ctgctgttac cttaacttgg
     7561 ggatagatag acaaaacgtc tgaaatatta ttgcgaagtt tattgatgaa acgcatacga
     7621 tttttgccct tggttgacag ctcaccgtag cgaatcataa tttctgaata ctgcatgaat
     7681 gctcctatct tacttttcta gtttgattgt aaattaattt tagcttggtc aagaactgct
     7741 cgacctgact catatcattt tcaaggtcta gactaagacg cacagctgac tgagccttat
     7801 ccttgtccac tcccatagca atcagggttc cggctggttt cccagccttg gacgaacaag
     7861 ccgaggtcgt tgagatgaaa atatcatagt cttcaaaggc gtgaacaatg acctcaccac
     7921 gaacaccctt gattccaaaa gtcagaatat gaggggcgaa gtcttcctca tctgaaaaga
     7981 caaatatatc cggatagtcc agaagtgctt gacgaatcac tgccttcatc tgcccagtct
     8041 tgctcctaaa gatatctagc ttttccatag ataaacggag agccttagcg gtcgctgcaa
     8101 ttcctgccac attttcagtt gtcgaacggt aatcacgttc ctgaccacca cctgttaaaa
     8161 gaggcgtaat cttcttgcca gacttgatat agataaaacc aacaccacgg attccgtgga
     8221 acttgtgacc agagaaagtc gcgaaatcca ctcgttctgt cagatacttt tcagtcggaa
     8281 tcttggcaag cgcctgaacc gcatcaacgt ggaaggaaat agttggcttg tctgccaaga
     8341 attctgaaat agcctcaata ggttggatag agccgatttc attgttcaca gccatgatgg
     8401 aaacgagggt cgtatcaggt cttatcaaat cttctagcgc ctcaacatcc acaaatcctt
     8461 tgttatcaac tggagcaaaa tccacttcaa aaccttgagc tttcaaccag agggctgatt
     8521 ccttaactgc tggatgttca atggctgata cgatgatgtg cttgccaaac tgggcttttt
     8581 caaatgctac tcccttgatg atccagttat ccccttctgt cccaccagat gtaaagaaga
     8641 tttcatcact tttcttaccg attaaatctg caatctgttg acgggaagca tctaaaattc
     8701 gtgttgcctg gtctcccaaa cgatggagac tagatggatt tcctaaaatt tttgaagcca
     8761 cctgcatata ggtttcaagg gcttctggat agggcctggt cgtcgccgaa ttatcaaagt
     8821 aaatcatgtt tctcacgctt tctaaaatca ctccttctat tgtatcatga aacggagcct
     8881 gcgacaagaa agggcaattt ctcttttttt aagatttttt taaagaaaca gagtataata
     8941 aatcataatc aaaggagaga atgtatgtct aactatcgta gaacttcaaa accgaaaaca
     9001 gaacatatca aaaaaggctt tactgttttt caaaaaacca ttactactat cggtagtatc
     9061 cttggtttga ttaccgcggg tatcacgatc atgaacgcct tggataataa taataaaaat
     9121 actaaaaaag aacctacgac aagccagacg acaacctttg tcaaagaaat tcaaaaagaa
     9181 tcccctcagg aaaatactac tccaaataag gaaaataaca cttctcaaga aaaaacacaa
     9241 caagaagaaa cgccaaaatc tagcgtcaag gaagagaaaa aagaagatca gaaaacagca
     9301 actcaggact ctactacacc tgctacaagt aaacctgcca ctgaaaatga aaaacagccc
     9361 aatactccaa cttcagaaaa taatactcaa tgaaaatcaa agagcaaact aggaagctag
     9421 ccgtagacag tacttgagta cggcaaggcg aagctgacgt ggtttgaaga gatttgcgaa
     9481 gagtataaaa gtaatcaata gccagtaaaa tagctccttc caaccttgga aagaagctat
     9541 tttttattgc tgcaatactt ttcttggctt ggtaccttca gctggaccaa tgacacctgc
     9601 catctcaagc tcttccatga gacgggtcgc acggttaaat ccaactgaca aacgacgctg
     9661 aatcatggat gcactggctt tctgtgtttc gataaccaaa gacttagctt cttcaaaaag
     9721 cggatcacca ccagcatctc catccgaaaa ttctccttca ttttcagaaa cctcacctgg
     9781 atcaaaactc tcatcgtagt ctgcatctgc ctgagtcttg atgaagttca caatgcgctc
     9841 aacatcgtca tccgagataa aggagccttg gagacgaact ggatgatttt catcaatcgg
     9901 tttaaagagc atgtctcctc gaccaagaag tttttctgct ccattttcat ccaaaatcgt
     9961 acgggagtct gttcctgatg aaaccgcaaa tgctacacga gatggaacat tggccttaat
    10021 caaaccagag atgacatcaa cagatggacg ctgagttgca agaatcatgt ggatacctgc
    10081 agcacgcgcc ttctgcccaa gacggatgat agcatcttcc acttccttgc tggccaccat
    10141 catgaggtca gccaactcat ccacaatcac gacaatgaat ggtagcggaa tttgcttgta
    10201 ctcagactgg gaattgaact cttctacctt ggcattaaaa cctgcaatat tccgaactcc
    10261 caccttggca aagagttcat aacggttttc catttcatcc acaacctttt gcagagcctt
    10321 gctggctttg cgtggattgg tcacgactgg aatcaagagg tggggaatat cattgtaaac
    10381 agataactca accatcttgg gatcgaccat cataaattta acttgatctg gtctcgcctt
    10441 catgagaatg ctagcaataa tgccgttaac tgctactgac ttccctgaac ccgttgaacc
    10501 tgcaactagc aagtggggca ttttagaaag gtcaaaagct cttgcggttc cattaacagc
    10561 cttccctaaa ggaatttcca agaaattttc tgctttcgtt tgcgattgtt cccatagttc
    10621 tcggaaagat acagtggcaa tatcggagtt gggcacttca attccgatta gggatttccc
    10681 agggattggt gcttcaatcc ggacatcttt ggcagccaag gctagagcga ggtcatctga
    10741 tagattggaa atgcggttga cccttacacc aacagccggc ttgacttcat acttggtcac
    10801 tgatggccca atttcggccc gttcaactgt taccttaata ccaaagctag caaaggttgc
    10861 ttctaagatt ttgatatttt ctctgacaat tttcttctct ttagactgat cttttggttt
    10921 atctggtgca aagagttgta agcttggaag tttgtattca agggcttctt tggctgaaaa
    10981 atcgacctga acatcttcgt catctgagtc atcttcctgt tcagggaatt taagttcagc
    11041 ttgaggcagg atgatttctg gttccaccca cttttcttct ggaataggtg gaagattttg
    11101 aacagcttcc tctgtcagaa tttcacccgt ttccatatca acaggaggca aatcgagtaa
    11161 ggctttttca gtctcttctt gttctaatct agcctctttc tcagcctttt ggcgagcttt
    11221 ttcttcttgt ttgacaaagc gttcctcttt tcgacgctcg tgcccttccc accatttggc
    11281 aaagcctcta ctgaaaaatt cagcaatatc gtaaacagac caagggctga ctaggagaga
    11341 acccactaaa atcaagatag aaccaataaa gtaagttccg atatttgaaa agagaaaagc
    11401 tgttggaata taaagagcga ccccgatcaa gccccctcca gcaaaactag tcgttcgaaa
    11461 accagtcaga tctgtcacaa cctgagccat ggtccctttt agaacggact tgtccaaacc
    11521 atatttccaa accaagtagg cttcaaaaat caagagtaag ccagcaaata tggtgaaaaa
    11581 gccagataag agtccttcct gttttcgtat ccacttgaaa aaaaagagat agattaatag
    11641 gccgaatatc gccagataag ctaggctacc cactagcaag cgaattaaat tataaagggt
    11701 tatacctgca gcccctaatt tgaaggctgc gaaaatcaat aaaatcgcaa ttcctaacga
    11761 aatcaacatt cgttgaatcg cttcttttct ttccagttct gctttagacg gtctccgtct
    11821 tgttgtactt gtattcttgt ttgccattct tctattatac catatttcac aggcatttcg
    11881 gaaagagaaa aagactgcac caaacggtcc aatcttttca tttttctatt ttttatgctt
    11941 gtggtttgcg taggtaacca taaaccacac cacttacgat tgctcctacc aagacagaaa
    12001 cgaggtaaag gagagcattt gaagtaaggg cgataacgaa gattcctccg tgtggcgcca
    12061 tgagtttaat accagtaaga ccaacgagtc cacctgctac tgctgaacca aggatgaagc
    12121 ttggaatcgc acgagctggg tcagcggcac caaatggaat cgctccctca gtgataaatg
    12181 acaagcccat gatgatgttt gtcaaaccag agttacgttc ttccttagta aatttatctt
    12241 tgaaaagaag agttgcgaca aagattgcaa gtggtggcac cattcctcca gccataactg
    12301 ctgccatggc tacagaacca cctgaagaaa cagttgctgc aagcgtacct gtaccaaaga
    12361 cataagctgc tttattaact ggtccaccca tgtcaacagc catcattcca ccaaggacga
    12421 taccaagaag gacagctgaa cctcctccaa gaccgcctag gaagtcattc atagcagtgt
    12481 tgattgctgc cattgggata ttcacagcta gcataacaaa tcctgtcaag attgttccaa
    12541 gaagtggcaa tagaaggatt gatttagcac cttcgagtga acgaggaact ttaacgtatt
    12601 tcttgatggc aagaaccaag gcacctgcga taaatccacc aacaagggca cctaggaaac
    12661 cagatgagac acctgcaaga gttgaagttg cttcaccacc tgcggcataa ggaattttac
    12721 caaaggcaaa accttctttg gcaatagcac cagccacgaa acctgctacc aaacctggtt
    12781 tttcagcaat agagtaggca acataacccg caaagactgg aagcatcaaa ccaaaggcag
    12841 ctccaccaat tttcatgaac atagaagcta actcatggta agaaccaaga ttgccaaggt
    12901 tttcatttgg aacacccaaa gcaccgtcaa tcaagaaggc aagggcaatc atgataccac
    12961 caccgataac gaatggtaac atttgagata caccactcat caagtgtttg tagaaggcac
    13021 caccaaggct ttgtttttcg ttagaggctg ttgcagcttt tgcaccatta gcggcacggt
    13081 agacttcagt atctcctgaa agagccaagt taattagctc ttctgtctta cggataccgt
    13141 cagcaactgg acgattgatc aatggttttc catcaaatcg atccatttca acggccttgt
    13201 ctgctgcaat gataatagct ttagccttac ggatatcttc tgcagttagt tgatttccaa
    13261 caccgctagc accgttggtt tcgaccttga taccaacccc catttcagca gctacttttt
    13321 gaagggcttc ttgggccatg taagtgtggg caatacctgt tgtacaagct gtaacagcta
    13381 cgataaagtc accagagtca ttagcaggta cttgaacaag ttcctcagtt ttttctgaag
    13441 cttggtcaaa aagttcgata acttggtctg cagatgttgc ttgacgaagt ttgtctgcaa
    13501 aaccgtcttt catcaagtat tgagacaatt ctgccaaggc tgccaagtga gtatcattgg
    13561 caccttctgg agctgcaatc atgaagaaga ggtcagttgc ttgtccatcc aagctctcgt
    13621 agtcaacacc cttatttgac ttagcaaata gaactgtcgc ttctttgaca gcagcgtttt
    13681 tgctgtgagg cattgcgatt ccatcaccca aaccagtaga agtcaaagct tcacgcgcca
    13741 aaattccttc tttaaatgtt tcaaaatctg ttacataact gtgctctgtc aaatttttaa
    13801 tcatctcgtc gatgacagct gttttttcag ttgcctgcaa atctagcaac atgacatctt
    13861 ttctcaatag gtcttgaatt ttcatctttt ttctacctca acttttccat atgtttcttt
    13921 aataaattcc gccgttgcca agtcatctga gaaggtagtt gccgttccgc aagccactcc
    13981 ccatttgaag gcttctactg cgtcttttga tttgacaaat tcacctgtga atccagcaac
    14041 catagaatca ccagctccaa ctgaattttt gactgttcct ttgattggtt tagcgaagta
    14101 agctccctca gatgtgacaa gaagggcgcc atcaccagcc atagagataa taacattttg
    14161 agcaccctta gccagtaact cacgagcgta tttctcaatt tcatctaaac tttcgagttt
    14221 aaccccaaaa atcgctccaa gttcatgatt gtttggtttt acaagaagag gctggtagtc
    14281 caaactatca attaaggtct gtccttcaaa gtcacagacc acttgcgcac cagtctggcg
    14341 cgtcaaggaa atcaaatcct tatagataac attgcctaga tttttagcac ttgaacctgc
    14401 aaagacaact gtatcttctg ctgtcagact agataaaata gctttcaatt cttctagctg
    14461 aaccggttca acagttggac ccgttccgtt gatttctgtt tcttggtctg ctttgatttt
    14521 aacattgata cgagtatctt ctgccacctg gacaaaacgt gtctcgattt cttcctctgc
    14581 taaagtatct gtgataaatt taccagtaaa gccaccgata aatcccgtcg ctgtatttgg
    14641 tatattcaaa cgtttcaaga cacggctgac attgattcct ttcccaccag caaacttatc
    14701 atcactgtcc atacgattta cactaccaac tttgacttgg tccaaacgaa cgatatagtc
    14761 aatggatgga ttgagtgtga ctgtataaat catacttcta ttacctccgt tttctcctta
    14821 ataacctgca agagctcatg cccttgacta gtgataacga tagcgcgttt gagtggtgct
    14881 accttggcaa agcaagtttg tccaattttt gacgaatcca ccaagacgta ggtctgcttg
    14941 gcattctcca aaatagctct tttcacagct ccttcctcca tatcaggagt cgtataatag
    15001 ccatcgtcaa caccattcat tccgataaag gcacggtcaa agtgcaattg gttaatctgg
    15061 ttaagagcaa cgccaccgat actagcatct gtcgccgtct tgacgtttcc tccaaccatg
    15121 acagttggaa tctgcttttc aaccaactga gcggcatggt gaatggagtt ggtcacaact
    15181 gtaatattct tattgaccaa ttcatgaatc aaaaaagcag ttgttgttcc agcatcgata
    15241 aagatgacat ctttttcttt aatgagagag gctgctttct gagccagcaa cttcttttct
    15301 tgaaggtttt tgacagattt ttcttgaatg gtttcttctt cctgtaagga gtgggggagt
    15361 tctgctccac catgcacacg acgaagcttg ttttccgctt ccaactcatc caagtctctt
    15421 cgaaccgttg attctgacgt ttctagcaaa ctaactaatt tttctagaga aactacatga
    15481 tgttgattta actcctctaa aatcagttgc ttccgctctg tttttaacac cgaatcacct
    15541 cctgttatcg tttacactat tcattctatc atactttctt ttgaagtcaa gcatttttta
    15601 tcattttctt tcaaaatcta tcatttttct tatttctaga atttttattg caagataaga
    15661 gcctttatgg tagactattt gagtaagtat tggaagatta ctcaagaggc ttaagaggcc
    15721 gtgttggaaa cgcggtaggc gtgtaatagc gtgcgtgggt tcgaatccca tgtcttccgt
    15781 cgaaaaaaga ttatttttag gatgcgacaa atgttgtatc cttttttcta tccttttttt
    15841 aaaatagggt ctgtttgtga ctcccttttt agcaaaagaa tgcaaatgaa taccaaagat
    15901 aaaaataaaa aacgttgatt taacaacgtt ttaccaagga atgcaaaaga atgcaaagga
    15961 ataatggagc cggtgggagt cgaacccacg tccaaacacc tgccaacata tttgtctaca
    16021 accataggtt atgtattatt ttaacagccc ctcgacacat aactcaagtc tagaaactgc
    16081 gagtctatca atctcttatc aaaccgctag acaaggctta atcgtatctc gctaataata
    16141 agacctgtca ttgaacacga gcaatccaaa tcgggtcacg cctgctggtt tttaggcagc
    16201 tagagcgtaa gaagtgttat tttttgcagt tatatttaac tgagcgttta cgtcgccaca
    16261 cgagtcgcaa aatatgcctc ataatgcctg tcgaatccgt aacgacccca aaagacaata
    16321 aaaccattat accttatcta actcaaaaat gcaaaaagaa atcacatacc taaaaaagat
    16381 actattcgag aaatttagca aactggaaga ctaactacaa ctttctcagt gcataacttt
    16441 acgattgtaa atagagctta cgaaatccaa ttaacacata cctaagatga agctatctgt
    16501 tttgaagaaa ttcacgaaga gaattattct aaaatacttt cagatgagaa ttttcgtact
    16561 ctctttattt taattcatt
//
LOCUS       DAVLSL010000034        15988 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLSL010000034 DAVLSL010000000
VERSION     DAVLSL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.034"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            179..613
                     /locus_tag="VXN30_000861"
     CDS             179..613
                     /locus_tag="VXN30_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1357515.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISSEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLVMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            625..1815
                     /locus_tag="VXN30_000862"
     CDS             625..1815
                     /locus_tag="VXN30_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV1357516.1"
                     /translation="MIKKVTIEEIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELAHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGITRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1826..2317
                     /locus_tag="VXN30_000863"
     CDS             1826..2317
                     /locus_tag="VXN30_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1357517.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKNVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2332..3111
                     /locus_tag="VXN30_000864"
     CDS             2332..3111
                     /locus_tag="VXN30_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1357518.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            3098..3916
                     /locus_tag="VXN30_000865"
     CDS             3098..3916
                     /locus_tag="VXN30_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1357519.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3916..4209
                     /locus_tag="VXN30_000866"
     CDS             3916..4209
                     /locus_tag="VXN30_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1357520.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4231..6132
                     /locus_tag="VXN30_000867"
     CDS             4231..6132
                     /locus_tag="VXN30_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1357521.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLIDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6192..7193
                     /locus_tag="VXN30_000868"
     CDS             6192..7193
                     /locus_tag="VXN30_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1357522.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7303..7638)
                     /locus_tag="VXN30_000869"
     CDS             complement(7303..7638)
                     /locus_tag="VXN30_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357523.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7649..7891)
                     /locus_tag="VXN30_000870"
     CDS             complement(7649..7891)
                     /locus_tag="VXN30_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357524.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7907..8101)
                     /locus_tag="VXN30_000871"
     CDS             complement(7907..8101)
                     /locus_tag="VXN30_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1357525.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            8267..9217
                     /gene="rsmH"
                     /locus_tag="VXN30_000872"
     CDS             8267..9217
                     /gene="rsmH"
                     /locus_tag="VXN30_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1357526.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            9229..9546
                     /gene="ftsL"
                     /locus_tag="VXN30_000873"
     CDS             9229..9546
                     /gene="ftsL"
                     /locus_tag="VXN30_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1357527.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9550..11802
                     /locus_tag="VXN30_000874"
     CDS             9550..11802
                     /locus_tag="VXN30_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV1357528.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAVFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGKDGIITYEKDRLGNIVPGTEQVSQQTVDGKDVYTTISSTLQSFMETQ
                     MNVFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGLTKDFVWRDILYQSNYE
                     PGSTMKVMTLAAAIDNNTFPSGEYFNSSELKIADVTIRDWDVNDGLTTGRMMTFLQGF
                     ALSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SQTRTNMILVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLTGST
                     NYIFSVVSMHPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDKVTTESSYAMPSIKDISPGELAEALRRNIVQPIVVGTGTKIKETSVEEGTNLAP
                     NQQVLLLSDKVEEIPDMYGWKKETAETFAKWLDIELEFEGSGSVVQKQDVRTNTAIKN
                     IKKIKLTLGD"
     gene            11804..12784
                     /locus_tag="VXN30_000875"
     CDS             11804..12784
                     /locus_tag="VXN30_000875"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1357529.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIAAVVVSFLVALFSKQLTNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIIFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVGYFKMTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            13342..15447
                     /locus_tag="VXN30_000876"
     CDS             13342..15447
                     /locus_tag="VXN30_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1357530.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AYYRATGQLPGNAESDVQMQQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIVSIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            15908..15988
                     /locus_tag="VXN30_000877"
                     /pseudo
     CDS             15908..15988
                     /locus_tag="VXN30_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4900 a         2814 c         3634 g         4640 t
ORIGIN      
        1 aaaaaggttt gttttcctaa aaaaacggaa gattttgaaa tttctgccta ttaaaatggt
       61 taaaaagata atgaaagcgc taatattttt ttaaaaaaac tattgacaag ataaaaataa
      121 aggattacaa tggggaatat aaagtaaacc ggtaaaccta aacaagaaag gagaaaagat
      181 gaaaattgta cttgtagggc atggacattt tgctacaggg atttatagtt ctttacaatt
      241 gattgcaggc aatcaagaaa atgtggaggc gattgacttt gtggaaggaa tgtcagcaga
      301 tgaactcaag caaaaaatct tacttgcaat ttcaagtgaa gaagaagttt taatcctaag
      361 tgatctcttg ggaggatcgc cattcaaggt ttcttctacc ataatgggag aaaatccagc
      421 caaaacaatg aatgttctct cgggtttgaa cttagtcatg ttaatggaag cagtctttgc
      481 tagaatggct catagctttg atgaggttgt taataaatca gtagtggcgg cccagggcgg
      541 agtcgtaaat ggtaaagaat tgttttcaac ggatgcagag gaagaggaag aagatttcga
      601 atcgggtatt taaagggtaa aagaatgata aaaaaggtta cgattgaaga aataaaatcg
      661 cctgagcgct tcttagaagt accacttctg acgaaagaag aagtcggcca ggcaatcgat
      721 aaggttattc ggcagttaga actcaacctt gactatttca aggaagattt cccgacgcca
      781 gctacctttg ataatgtcta tccaatcatg gataacacgg aatggaccaa tggtttctgg
      841 acaggagaac tgtggttggc ttatgaatac agtcaacagg atgcatttaa aaacatcgct
      901 cataaaaatg ttctttcttt cctggatcgt gtcaataaga gagtagaatt ggctcaccat
      961 gatctcggct tcttgtacac accgtcttgt atggctgaat ataagataaa tggagatgga
     1021 gaggctagag aagcagcctt gaaagctgca gataagttga ttgaacgcta tcaagaaaaa
     1081 ggtggtttta ttcaagcttg gggagacttg ggcaagaaag agcattaccg tttgattatc
     1141 gattgcttgc tcaatatcca actcttattc tttgcttatc aagaaacagg cgatcaaaaa
     1201 tactacgata ttgcagaaag ccatttctat gcttcagcta ataatgtaat ccgtgatgac
     1261 gcttcgtcct tccacacctt ctattttgat cctgagacag gtcaaccctt taaaggtata
     1321 acgagacaag ggtatagtga tgattcatgc tgggcacgtg gtcagtcatg gggagtttat
     1381 ggtattcctt tgacttatcg tcacttaaaa gacgagtcct gctttgactt gtttaagggt
     1441 gtgaccaatt atttcttgaa tcgtctgcca aaagatcatg tgtcctattg ggatttgatt
     1501 tttaatgatg gtagtgatca atcacgagat tcttcagcaa cagctatcgc cgtctgtggg
     1561 attcatgaaa tgctaaaaca tctcccagag gtggatgctg acaaagatat ttataaacat
     1621 gctatgcatg ccatgcttcg ttccttgatc gaacattatg caaatgatca atttacccct
     1681 ggtgggacaa gtctcctcca cggtgtgtac tcatggcatt caggtaaagg agtggatgaa
     1741 ggcaatatct ggggtgacta ctattaccta gaagccctta tccgtttcta caaagactgg
     1801 aacctatatt ggtaggagga gaaatatgac aatgccaaat attattatga cccgtatcga
     1861 tgaacggttg attcatggac aaggacaact ttgggtaaaa tacctaggtt gtaatacggt
     1921 cattgttgcc aatgacgaag taagcacgga caagatgcaa caaactctga tgaaaacagt
     1981 tgtgccagac tcagttgcca tgcgtttctt ccctttgcaa aaggtgattg atatcattca
     2041 caaggctaat cctgctcaaa cgatctttat cgttgtaaag aatgtgaagg atgctttaac
     2101 cttggtagaa ggtggtgtcc ctatcaaaga aatcaatatt ggaaacattc acaatgctac
     2161 tggtaaagag caagtgacac gctccatctt cctgggtgaa gaggacaagg cggccctcaa
     2221 ggaattgagc caaactcatc aagtaacatt taatacgaaa acaactccaa caggaaatga
     2281 tggagctgtt caagtcaaca ttatggacta tatttaaaag aggagatcgt tatgtcgatt
     2341 aatgtatttc aagcgatttt aattggatta tggacagctt tctgttttag tggaatgctg
     2401 ttaggaattt acaccaatag atgtattgtt ctgtcatttg gtgtcggaat tattctaggt
     2461 gatctgccta ctgctcttgc aatgggagct attggtgaat tggcttatat gggattcggt
     2521 gttggtgctg gaggtactgt tccaccaaac ccaatcggac ctggtatctt tggtaccttg
     2581 atggctatca ctagtgctgg taaagtcagt ccagaagcgg ctcttgccct ctctactccg
     2641 attgctgtgg cgattcaatt cttacaaact ttcgcctaca ctgtacgtgc tggtgcgcct
     2701 gaaacagcga tgaagcactt gaaaaaccat aatttgaaga aattcaagtt cactctaaat
     2761 gcaacaattt ggttgtttgc ctttattgga tttaccttgg gttgcttggg tgccctttca
     2821 atggatacct tgttgaaact cgtagactac attccaccgg tattgcttac aggtttgaca
     2881 gttgctggta aaatgctccc agctatcggt tttgcgatga tcttgtcagt gatggctaag
     2941 aaagagttga ttccctttgt cttgttggga tatgtttgtg cagcttatct aaacatccca
     3001 acaattggta ttgcaattgt aggtactatc tttgctttga ttgaatttta taacaagcca
     3061 aaaacagcgg atcatgtggt agaggaggaa gcacacgatg actggatcta acaaattaac
     3121 aaaacgtgat tatcttaaaa cgtctttgcg ggcattcttt tgtcaaaatg gatttaacta
     3181 tagtaactat caagggttgg gatatgccaa tgtgatgtat cctgctttga aaaaacacta
     3241 tggagaggat caggaaggtt tctaccaagc cttggaagaa aactgtgaat tctataatac
     3301 caacccacac ttcctgcctt ttattaccag cttgcatctt gtaatgttgg aaaatggccg
     3361 tccggcaaaa gaaacacgta gcatcaagat ggccttgatg ggaccattgg caggtattgg
     3421 ggattctctt tctcaattct gtttagctcc attgttctca accatcgcag cttcgtttgc
     3481 tcaagaaggc ttggttgtcg gtccaatctt gttcttcctt gcgatgaata cgattttaac
     3541 agcgattaaa ttgtcaactg gtctgtatgg atacaaacta ggaacaactg tgattgataa
     3601 actaagcgaa cagatggcaa cgatttctcg tattgccaat attatcggtg taaccgtaat
     3661 tgctggtttg gcagcgacat ctgttaagat tatggtgccg attacctttg ctgcagggga
     3721 agttaaagca gacgctaaac aaagtatcgt aagtattcag ggaatgcttg ataaggttgc
     3781 tccagctctt ctaccagccc tatttacact tttagtttac tacttgatca aagaaaagaa
     3841 atggacaaca tataaactcg ttattttaac agttatcatc ggaattatcg gaagctggct
     3901 taagattata gcttaatgga tacaacattg ttttatggaa tagtgattgt cttggcagtg
     3961 agtccccttt tactgtcaag ctttcattct attcgtcagc aaaagttgct tcgcaaacag
     4021 atggagcaac gacaagagta tttagcttct ttaacatctg gtgatgaagt gttgttattg
     4081 tctggaattc atggaaaaat catttctatc caagatgact tggtctcctt gcagattgca
     4141 aaaggtgtgg tcatctatgt agaaaaggaa agtgtaatgg gaaagacaaa agaactgctt
     4201 tttaagtagt tctttttctg gtaatagata atgaaagaag aaagaagaca attttttgaa
     4261 agagtcgatg gaaaccaatg tcgtgattat atcttgtccc actgttcaaa agactatgag
     4321 aaagtcaaga gttcccttga acgcttgata gacaatcgtt ttatgtttga tagtccttgg
     4381 gatatggagc cttgttcaaa aattcatcaa atccagccga tggtatggga tcaagtattt
     4441 gaagatgatc cagaatggtc ttatatgctc aatcgacaag aatatctctt gcagtttatg
     4501 atagggtatc tggtagaagg agataagggc tatatccaaa agtgcaagtt ctttctattt
     4561 gattggattg agcaggtgag agaattttct cctcaatcct tgatgactag aaccttggat
     4621 acgggtattc gttcctttac ttggttgaaa ctactcttgc tcctcttgaa atttgacttg
     4681 ctagaggaga aagaactaga gaaaattttg gtcagtctag aaaagcagat tgactttatg
     4741 aaaagccact atcgcgccaa gtacaccctt agtaactggg ggattttaca aacaattccg
     4801 atgcttgcta tctattattt cttttcagat aagatggacc tagaagaagc ttaccatttt
     4861 gcttcagagg agttgaaaca gcaaattgag acacagattt taggagatgg aagccagttt
     4921 gaacagtcga ttctctatca tgtagaggtt tataaagcct tgctggattt gtgtctcttg
     4981 cttccagact tgcaagatag ttaccgagag ttgctggaaa agatggcgac ctatattcaa
     5041 atgatgacag gcttagatgg acgaactttg gcttttggtg atagcgattc tacagaaacg
     5101 acagaaattt tgagcctgtc tgctgtggtt ttgaacaagg aagaccttct taacggtctg
     5161 gatgttaaag tcgatttgct tagcctcttg ttcctggggc gagaaaaggt caagcgactg
     5221 caggaatttg aaaggagagc ttggcagcct aagtccatga tctttgaaga ctctggacat
     5281 gtctgcatta aggatgaaca tcgttatcta tttttcaaaa atggtccgct aggaagtgcc
     5341 catagccata gtgacgagaa tagtttttgc ttacagtatc aaggccaacc tattttcata
     5401 gatgctgggc gttattctta tcgggagata tatgaacgtt atctcttaaa gagtgcttgg
     5461 agtcattcga cctgcattgt agatgggaaa gctccggaaa gaatcacggg atcctgggaa
     5521 tatgaatact atcctcactc cctgttttgt caccataaag aaagggaggg agtgcattat
     5581 attgaggggg cttattggtc agcagaacct gatttgcctt atcttcacaa gagaaaaatc
     5641 ctcatgttgg tagaggatgt ctggctcttg gtagatgaca tcaggtgtca aggtcagcat
     5701 gaggcgttga ctcagtttat ccttgacaag gatgtgacct atcaagatgg gaaaatcaat
     5761 cagttgagac tatggagtga agttgatttt gatttggaag ataccatcat ttctcctaaa
     5821 tacaatgagc ttgaaagaag tagcaaactc accaagcgcc aattctttga gaatcagatg
     5881 ctggattata ccatcattgc gcatgagagt tttgaaatta tccgtcattc tgtctaccag
     5941 acagatgatc gtgaagtgga aaatgctctg gcttttgaag tgaaaaatga cgaaacagac
     6001 aagctgattc tgttattaag cgaggatatt ggtgtaggtg aaaaattgtg cctcgttgac
     6061 ggaacaaaaa tgcgtggaaa atgtctagta tatgataaaa taaatgagag aatgattcgc
     6121 ttgcagtgct agaaataggc attttgaata gtgaatatgt tataataagt attagtagga
     6181 ggtgttttag attggagaag aaactgacca taaaagacat tgcggaaatg gctcagacct
     6241 cgaaaacaac cgtgtcattt tacctaaacg ggaaatatga aaaaatgtcc caagagacac
     6301 gtgaaaagat tgaaaaagtt attcatgaaa caaattacaa accgagcatt gttgcgcgta
     6361 gcttaaactc caaacgaaca aaattaatcg gtgttttgat cggtgatatt accaacagtt
     6421 tctcaaacca aattgttaag ggaattgagg atatcgccag ccagaatggc taccaggtaa
     6481 tgataggaaa tagtaattac agccaagaga gtgaggaccg gtatattgaa agcatgcttc
     6541 tcttgggagt agacggcttt attattcagc cgacctctaa tttccgaaaa tattctcgta
     6601 tcatcgatga gaaaaagaag aaaatggtct tttttgatag tcagctctat gaacaccgga
     6661 ctagctgggt taaaaccaat aactatgatg ccgtttatga catgacccag tcctgtatcg
     6721 aaaaaggtta tgaacatttt ctcttgatta cagcggatac gagtcgtttg agtactcgga
     6781 ttgagcgggc aagtggtttt gtggatgctt taacagatgc taatatgcgt cacgccagtc
     6841 taaccattga agataagcat acgaatttgg aacaaattaa ggaattttta caaaaagaaa
     6901 tcgatcccga tgaaaaaact ctggtattta tccctaactg ttgggcccta cctctagtct
     6961 ttaccgttat caaagagttg aattataact tgccgcaagt tgggttgatt ggttttgaca
     7021 atacggagtg gacttgcttt tcttctccaa gtgtttcgac gctggttcag ccctcctttg
     7081 aggaaggaca acaggctaca aagattttga ttgatcagat tgaaggccgc aatcaagaag
     7141 aaaggcaaca agtcttggat tgtagtgtga attggaaaga gtcgactttc taaaatgaag
     7201 gaaaatgact tgcaatttct gttaagaaat aaaataatcc cacctagaac aagctaggtg
     7261 ggattatttg cctatgaaat gagaaattat gggagcaagc tcctaaatca actgtttttg
     7321 atctacttct ttaactactt gataaaagtt atagaagtag gccaaacttg aaatgatggt
     7381 tacgactagg aatattgaaa atttccattg gacagggttg gttaaaagtt gtggaaagga
     7441 tatgaggaga aagaagaggg ctgcgttgag gacaggtatc cgttttgatt gtattttctc
     7501 aagtccttta ttgagcgcag gaagaaagag gagtaggagt agtaaaactg tatgagaaac
     7561 agctcctgaa ataagggcga agaaaaggaa aatactgata aaaacatgaa tgatcagtag
     7621 tctagctagt gatttcataa ggcacctcct aatcctggtc ttttttagct cttgcaatac
     7681 gaagtgagtc gacaatatgt atcatcactc cgaaaaagaa agctcccagt atagttttaa
     7741 aaatatgttt tgtatttaga agagaactga taaaatttgg attttcactt gttagggtat
     7801 caatgagtgg aattataaaa aatatcactg ttccataaat cgaacctgct ttcagaccag
     7861 gataacgtaa ctgtttcttt tcttttttca tgagtttgct ccttttttaa aaatcttcct
     7921 tccaaaagag actgttgagg tcagtttgga ggctgcgagc gagattgaga cagagttcca
     7981 gagttggatt gtacttgtcg ttttcaatca tattgatggt ctgtctcgag acaccgatat
     8041 ccttggcgag ctcgagctgg gaaatgccca gttccttgcg aaattctttc acacgattca
     8101 tctggtctcc tttctgattt gtgtcgtata tatttgactt tattatagtc ttttaaacat
     8161 aaagtgtcaa gtatttttga catatttttt gaagaaatag tagtctcctt gtcctatttg
     8221 tctgacaagt gcaagctggt cggatttgtg gtaaaataga taagatatga caaaagaatt
     8281 tcatcatgta acggtcttac tccacgaaac gattgatatg cttgacgtaa aacctgacgg
     8341 tatctacgtt gatgcgactt tgggtggagc aggccatagc gagtatttat taagtaaatt
     8401 aagtgaaaaa ggccatctct atgcctttga ccaggatcag aatgccattg acaatgcgca
     8461 aaaacgcttg gcaccttaca ttgagaaggg aatggtgacc tttatcaagg acaacttccg
     8521 tcatttacag gcacgtttgc gcgaagctgg tgttcaggaa attgatggaa tttgttatga
     8581 cttgggagtg tctagtcctc aattagacca gcgtgagcgt ggtttttctt ataaaaagga
     8641 tgcgccactg gacatgcgga tgaatcagga tgctagtctg acagcctatg aagtggtgaa
     8701 caattatgac tatcatgact tggttcgtat tttcttcaaa tacggtgagg acaaattctc
     8761 taaacagatt gcgcgtaaga ttgagcaagc gcgtgaagtg aagccgattg agacaacgac
     8821 tgagttagca gagattatca agttggtcaa acctgccaag gaactcaaga agaagggtca
     8881 tcctgctaag cagattttcc aggctattcg aattgaagtc aatgatgaac tgggagcggc
     8941 agatgagtcc atccagcagg ctatggatat gttggctctg gatggtagaa tttcagtgat
     9001 tacctttcat tccttagaag accgcttgac caagcaattg ttcaaggaag cttcaacagt
     9061 tgaagttcca aaaggcttgc ctttcatccc agatgatctc aagcccaaga tggaattggt
     9121 gtcccgtaag ccaatcttgc caagtgcgga agagttagaa gccaataacc gctcgcactc
     9181 agccaagttg cgcgtggtca gaaaaattca caagtaagag ggaaaaagat ggcagaaaaa
     9241 atggaaaaaa caggtcaaat actacagatg caacttaaac ggttttcgcg tgtggaaaaa
     9301 gctttttact tttccattgc tgtaaccact cttattgtag ccattagtat tatttttatg
     9361 cagaccaagc tcttgcaagt gcagaatgat ttgacaaaaa tcaatgcgca gatagaggaa
     9421 aagaagaccg aattggacga tgccaagcaa gaggtcaatg aactattacg tgcagaacgt
     9481 ttgaaagaaa ttgccaattc acacgatttg caattaaaca atgaaaatat tcgaatagcg
     9541 gagtaagata tgaagtggac aaaaagagta atccgttatg cgaccaaaaa tcggaaatcg
     9601 ccggctgaaa acagacgcag agttggaaaa agtctgagtt tattatctgt ctttgttttt
     9661 gccgtttttt tagtcaattt tgcagtcatt attgggacgg gcactcgctt tggaacagat
     9721 ttagcgaagg aagctaagaa ggttcatcaa accacccgta cagttcctgc caaacgtggg
     9781 actatttatg accgaaatgg agtcccgatt gctgaggatg cgacatctta taatgtctat
     9841 gcggtcattg atgagaacta taagtcagca acgggtaaga ttctttacgt agaaaaaaca
     9901 caatttaaca aggttgcaga ggtctttcat aagtatctgg acatggaaga atcctatgta
     9961 agagagcaac tctcgcaacc caatctcaag caagtttcct ttggagcaaa gggaaatggg
    10021 attacctatg ccaatatgat gtctatcaaa aaagaattgg aagctgcaga ggtcaagggg
    10081 attgatttta caaccagtcc caatcgtagt tacccaaacg gacaatttgc ttctagtttt
    10141 atcggactag ctcagctcca tgaaaatgaa gatggcagca agagtttgct gggaacttct
    10201 gggatggaga gttccttgaa cagtattctt gcagggaaag acggtattat tacttatgaa
    10261 aaggatcgtc tgggtaatat tgttcccgga actgaacaag tgtcccagca aacggtagat
    10321 ggtaaggatg tttatacgac tatttctagc acccttcagt cctttatgga aacacagatg
    10381 aatgtctttc aagaaaaagt aaaaggcaag tatatgacgg ctaccttggt tagtgctaaa
    10441 acgggggaaa ttcttgcaac gacgcagaga ccaacctttg atgccgatac taaggaagga
    10501 cttaccaagg actttgtttg gcgtgatatc ctttatcaaa gtaactatga gccagggtca
    10561 accatgaagg tcatgacgct tgctgctgct attgataata ataccttccc aagtggagaa
    10621 tacttcaata gcagtgaatt aaaaatagcg gatgtgacga ttcgagattg ggatgttaat
    10681 gatggtttga ctactggtag gatgatgact ttcttacaag gtttcgctct ctccagtaat
    10741 gttggaatga ccttgcttga gcagaaaatg ggagatgcga cttggctcga ttatctcaat
    10801 cgctttaagt tcggggtgcc gactcgtttt ggcttgacgg atgaatatgc aggtcaactt
    10861 ccggctgaca atatcgtaaa tattgctcag agttcatttg gacaagggat ttcagtaacc
    10921 cagacgcaaa tgattcgtgc cttcacggct attgccaacg atggagtcat gttagaacct
    10981 aaatttatta gtgccattta tgatccaaat gatcaaactg ctcggaaatc tcaaaaagaa
    11041 attgtgggaa atcctgtttc gaaagatgca gctagccaaa ctcggactaa catgattttg
    11101 gtagggacgg atccggttta tggaaccatg tataaccaca gcacaggcaa gccaactgta
    11161 actgttcctg ggcaaaatgt agccctcaag tctggtacgg ctcagattgc ggatgagaaa
    11221 aatggtgggt atcttacagg ttcaacaaac tacattttct cagttgtttc tatgcaccca
    11281 gcggaaaatc ctgactttat tctctatgta acggttcaac agcctgagca ttattcaggt
    11341 attcagttgg gagaatttgc caccccaatc ttggagcggg cttcagctat gaaagaatct
    11401 ctcaatcttc aatctccagc caaaaattta gataaagtta cgacagaatc ttcttatgca
    11461 atgcctagca tcaaggatat ttcacctggt gagttggcgg aagccttacg ccgaaatatt
    11521 gtgcaaccaa tcgttgtagg tactggaaca aagattaaag agacttctgt agaagaaggg
    11581 accaatcttg caccaaacca acaagttctc cttttatcgg ataaggtaga agaaattcca
    11641 gacatgtatg gctggaaaaa agagactgcc gagacctttg ctaaatggtt ggatattgaa
    11701 ctggaatttg aaggttcagg ttccgttgtt cagaagcaag atgttcggac taatacagct
    11761 atcaaaaaca ttaaaaaaat taaattaact ttaggagact aatatgttta tttctattag
    11821 tgctggaatt gtgacatttt tactaacttt agtaggaatt ccggccttta tccaatttta
    11881 tagaaaggcg caaattacag gccagcagat gcatgaggat gttaaacagc accaggcaaa
    11941 agctgggact cctacaatgg ggggacttgt tttcctcatt gctgcagttg tggtgagttt
    12001 cctcgtcgct cttttttcga aacaattgac caataatgtt ggtatgattt tgtttatttt
    12061 ggtcttgtat ggcttggttg gttttttaga tgactttctc aaggtcttcc gaaagatcaa
    12121 tgaggggcta aaccccaaac agaaattggc tcttcagctg ctaggtggcg tcattttcta
    12181 ccttttttat gagcgtgggg gcgatatgct ctctgtcttt ggctaccaag tgcatttagg
    12241 gattttctat attatcttcg ctcttttctg gctagttggt ttttcaaacg cagtcaactt
    12301 gacagatggt attgacggtc tagctagtat ttccgttgtg attagcttgt ctgcctatgg
    12361 agttattgcc tatgtgcaag gtcagatgga tatccttcta gtgattcttg ccatgattgg
    12421 tggtttgctt ggtttcttcg tctttaacca taagcctgct aaggtcttta tgggagatgt
    12481 gggaagtttg gctctcggtg gaatgttggc agctatctct atggccctcc accaagaatg
    12541 gactctcttg attatcggaa ttgtttatgt ctttgaaaca acttcggtta tgatgcaagt
    12601 cggctatttc aaaatgactg gtggtaaacg tattttccgt atgacgcctg tgcatcacca
    12661 ttttgagctt gggggattgt ctggtaaagg aaatccttgg agcgagtgga aggttgactt
    12721 cttcttttgg ggagttgggc ttctagcaag tctcctgacc ctcgcaattt tgtatttgat
    12781 gtaagaatgg caccctgatg tttcagggtg tttttgtgtt taaatacaca atgaaaatca
    12841 aagaacaaac tagaaagcta actttaggct gctcaaaata taatatattg aaactagaat
    12901 agtacacctc tacttctaaa acattgttag aaatcgattt gactgtcctg aacgatttat
    12961 cctgttctta tttcatttta ctatacagtt tcgaggttgt agataaggcg aagctgatgt
    13021 ggtttgaaga gattttcgaa gagataaaaa atattggtca attaaagtca aaatccttga
    13081 ccttttctga ctaatgtagt atattatgaa cgtaaggtaa atgaaagcct tactagaaca
    13141 cttatttaaa gtaaaataga aagaggtggg gtctatgttt catctagaaa tcttcagaag
    13201 taaagatagt ctactcctgc ttgaaaaaga aaaaccagaa atagtacata gggtagcggt
    13261 ttaactagtc taaatttttt aaagaaaagg tcaaagatag tcaatatcag taataataaa
    13321 taacaaaaag aggtaaagaa tatgaacaac aattttaata attttaacaa catggatgat
    13381 ttatttaacc aattgatggg tggtatgcga ggatacagtt ctgaaaatcg acgttacttg
    13441 attaatggac gtgaagtgac acctgaggaa tttgcttact atcgtgcaac tggtcaattg
    13501 ccaggaaatg cagaatctga tgtgcaaatg caacaacagg cttcaggtat gaaacaagat
    13561 ggtgtccttg ctaaactggg tcgaaacttg acagcagaag cgcgtgaggg caagttggat
    13621 cctgttatcg gacgaaacaa ggaaattcaa gaaacatctg aaatcctctc acgccgcacc
    13681 aagaacaatc ctgttttagt cggagacgca ggtgttggta agacagccgt tgtcgaaggc
    13741 ctagcgcaag cgattgtgaa cggcgatgtc ccagctgcta ttaagaacaa ggaaattgtt
    13801 tccattgata tctcaggtct tgaggctggt actcaatacc gtggtagctt tgaagaaaat
    13861 gtccaaaact tagtcaatga agtgaaagaa gcagggaata ttatcctctt ctttgatgaa
    13921 attcaccaaa ttcttggtgc tggtagcact ggtggagaca gtggttctaa agggcttgcg
    13981 gatattctca agccagctct ctctcgtgga gaattgacag tgattggggc aacaactcaa
    14041 gacgaatacc gtaacaccat cttgaagaat gctgctcttg ctcgtcgttt caacgaagtg
    14101 aaggtcaatg ctccttcggc agagaatact tttaaaattc ttcaaggaat tcgtgacctc
    14161 tatcaacaac accacaatgt catcttgcca gacgaagtct tgaaagcagc ggtggattat
    14221 tctgttcaat acattcctca acgtagcttg ccagataagg ctattgacct tgtcgatgta
    14281 acagctgctc acttggcggc tcaacatcca gtaacagatg tgcatgctgt tgaacgagaa
    14341 atcgaaacgg aaaaagacaa gcaagaaaaa gcagttgaag cagaagattt tgaagcagct
    14401 ctaaactata aaacacgcat tgcagaattg gaaaagaaaa tcgaaaacca cacagaagat
    14461 atgaaagtga ctgcaagtgt caacgatgtg gctgaatctg tggaacgaat gacaggtatc
    14521 ccagtatcgc aaatgggagc ttcagatatc gaacgtttga aagatatggc tcatcgcttg
    14581 caagacaagg tgattggtca agataaggcc gtagaagctg tagctcgtgc tatccgtcgt
    14641 aaccgtgctg gttttgatga aggaaatcgc ccaatcggca gcttcctctt tgtagggtct
    14701 actggggttg gtaagacgga gcttgctaag caattggcac tcgatatgtt tggaacccag
    14761 gatgcgatta tccgtttgga tatgtctgaa tacagtgacc gcacagctgt ttctaagcta
    14821 attggtacaa cagcaggcta tgtgggttat gatgacaata gcaatacctt aacagaacgt
    14881 gttcgtcgca atccatactc tatcattctc ttggatgaaa ttgaaaaggc tgaccctcaa
    14941 gttattaccc ttctcctcca agttctagat gatggtcgtt tgacagatgg tcaaggaaat
    15001 acagtaaact tcaagaacac tgtcattatt gcgacctcaa atgctggatt tggctatgaa
    15061 gccaacttga cagaagatgc ggataaacca gaattgatgg accgtttgaa acccttcttc
    15121 cgtccagaat tcctcaaccg ctttaatgca gtcatcgaat tctcacactt gactaaggaa
    15181 gacctttcta agattgtaga tttgatgttg gctgaagtta accaaacctt ggctaagaaa
    15241 gacattgact tggtagtcag tcaagcggct aaagattata tcacagaaga aggttacgac
    15301 gaagtcatgg gagttcgtcc tctccgtcga gtggttgaac aagaaattcg tgataaggtg
    15361 acagacttcc acttggatca tttagatgct aaacatctgg aagcagatat ggaagatggc
    15421 gttttggtta ttcgtgagaa agcctaagat agaattttga ggataaaaaa gaaggagcca
    15481 gctgaaaaaa actggttcct tttgaggtac gacgggcatg tcgtatagta ggttgaaact
    15541 agaatagtac acatctactt ctaaaacatt gttagaaatc gatttgactg tcctgaacga
    15601 tttgtcctgt tcttatttca ttttactata tatctgaata tctgaggtgt aagtcctcgg
    15661 tgggcaccca ctaccggtga acccaatagc gattcccaag cctgactatc gtgaggtagt
    15721 ggattaaaac ggtctgggga tagaccgttt taagtctgac gctggaaata agaatcgtca
    15781 gaggaaggga tagcgaaatc gtggctttac gaacaggaac gtgatagtaa ggcgtatata
    15841 gcggataagg gggtctcaaa ctctaaagtt caaaaaggta gtcgtaacct atatgcgtaa
    15901 atcacgagag caactgaatt tgttcattcg aggatggata aactatttct cattgggaaa
    15961 tatgaaaagt atagtcgcca gcatagat
//
LOCUS       DAVLSL010000035        15281 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLSL010000035 DAVLSL010000000
VERSION     DAVLSL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.035"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..552
                     /locus_tag="VXN30_000878"
     CDS             <1..552
                     /locus_tag="VXN30_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1357531.1"
                     /translation="IVQHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKL
                     SDKQFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIED
                     NLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFEN
                     FKKRIFIALNIKKERTKFVLSRA"
     gene            complement(652..2274)
                     /gene="groL"
                     /locus_tag="VXN30_000879"
     CDS             complement(652..2274)
                     /gene="groL"
                     /locus_tag="VXN30_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1357532.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2290..2574)
                     /gene="groES"
                     /locus_tag="VXN30_000880"
     CDS             complement(2290..2574)
                     /gene="groES"
                     /locus_tag="VXN30_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1357533.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2844..3239)
                     /locus_tag="VXN30_000881"
     CDS             complement(2844..3239)
                     /locus_tag="VXN30_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1357534.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3316..4077)
                     /locus_tag="VXN30_000882"
     CDS             complement(3316..4077)
                     /locus_tag="VXN30_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1357535.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTHCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4110..4736)
                     /locus_tag="VXN30_000883"
     CDS             complement(4110..4736)
                     /locus_tag="VXN30_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1357536.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVACLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPVKHWTA"
     gene            complement(4752..5069)
                     /locus_tag="VXN30_000884"
     CDS             complement(4752..5069)
                     /locus_tag="VXN30_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1357537.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5066..5365)
                     /locus_tag="VXN30_000885"
     CDS             complement(5066..5365)
                     /locus_tag="VXN30_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1357538.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5702..5893)
                     /locus_tag="VXN30_000886"
     CDS             complement(5702..5893)
                     /locus_tag="VXN30_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1357539.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6054..6728)
                     /locus_tag="VXN30_000887"
     CDS             complement(6054..6728)
                     /locus_tag="VXN30_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357540.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLRVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6734..7180)
                     /locus_tag="VXN30_000888"
     CDS             complement(6734..7180)
                     /locus_tag="VXN30_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1357541.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLIF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7294..7797)
                     /locus_tag="VXN30_000889"
     CDS             complement(7294..7797)
                     /locus_tag="VXN30_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1357542.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7794..8156)
                     /locus_tag="VXN30_000890"
     CDS             complement(7794..8156)
                     /locus_tag="VXN30_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1357543.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8222..10081)
                     /locus_tag="VXN30_000891"
                     /pseudo
     CDS             complement(8222..10081)
                     /locus_tag="VXN30_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(10120..10569)
                     /locus_tag="VXN30_000892"
                     /pseudo
     CDS             complement(10120..10569)
                     /locus_tag="VXN30_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(10693..11409)
                     /locus_tag="VXN30_000893"
     CDS             complement(10693..11409)
                     /locus_tag="VXN30_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1357544.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12290..13705)
                     /gene="ply"
                     /locus_tag="VXN30_000894"
     CDS             complement(12290..13705)
                     /gene="ply"
                     /locus_tag="VXN30_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1357545.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13716..14048)
                     /locus_tag="VXN30_000895"
     CDS             complement(13716..14048)
                     /locus_tag="VXN30_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357546.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTGIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14119..14727)
                     /locus_tag="VXN30_000896"
     CDS             complement(14119..14727)
                     /locus_tag="VXN30_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357547.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14740..15198)
                     /locus_tag="VXN30_000897"
     CDS             complement(14740..15198)
                     /locus_tag="VXN30_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1357548.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
BASE COUNT         4451 a         3535 c         2769 g         4526 t
ORIGIN      
        1 attgtacaac atcttagccg tgctatgagt cgtgtgcgtg tccaaatcat gaatcagttt
       61 catcgaaaat cccatgaata caaggctatc aagcgctact ggaaactcat tcaacaggat
      121 agccgtaaac tgagtgataa gcaattttat cgtcctactt ttcgtatgca tttaaccaat
      181 aaagagattt taaacaagct tttgagctat tcggaagact tgaaacatca ctatcagctc
      241 tatcagctct tgctttttca cttccagaat aaggaaccag agaaattttt cggacttatt
      301 gaggacaatc ttaagcaggt tcatcctatt tttcagactg tctttaaaac cttcctcaaa
      361 gataaagaaa agattgtcaa cgcccttcaa ctacactatt ctaatgccaa actggaagcg
      421 accaataatc tcatcaaact tatcaagcgc aatgcctttg gttttcgaaa ctttgaaaac
      481 ttcaaaaaac ggattttcat cgctctgaat atcaaaaaag aaaggacaaa atttgtcctt
      541 tctcgagctt agcttttctt caacccacta cagttgacaa agagcctata aaaagaaggg
      601 ttagtcattc cctccttttg tgttttttat aagttgtttt ctatagaaag cttacatcat
      661 cccgcccatc atacttggat ccattgctgg agctggggct actggttctg gtttattggc
      721 tacgactgct tctgttgtca aaatcaagct ggctacagat gctgcatttt gtagggctga
      781 acgactcact ttaactggat caatgatacc ttgatcaatc atgttaaccc actcgccagt
      841 tgctgcgttg aatcctatac caagctcagc atttttcaaa cgatcgataa cgatagatcc
      901 ttcaaatcct gcattgtgag caatttgacg aacgggttct tccaaagcac ggagaacaat
      961 attacgtcct gttgcttcat ctcctgtcaa ttccaaggta gcaacagctg gaatcacatt
     1021 ggcaagagct gttccaccac ctgcaacaat accttcttca acagctgcac gagtagcgtt
     1081 gagggcatct tcaatgcgga gtttcatttc tttcaactca gtttcagttg cggctccgac
     1141 cttaataacc gctacaccac ctgacaattt ggccaagcgt tcttgcaatt tttcacggtc
     1201 aaattcagaa gttgtagttt cgatttgaga cttgataacc gcaacacggt tagaaatcgc
     1261 ttcaggattt cctgcacctt ctacaataac cgtgctatct ttgtccacgg tcactctcgc
     1321 tgcttgacca agagcttcaa ttgtcgcatc tttcaactca agaccaaggt cttctgtgat
     1381 aactgttccg cctgttaaga tggcgatatc ttcaagcatg gctttgcgac ggtcaccaaa
     1441 accaggtgcc ttgactgcta ctacgttgaa ggttccacga atcttgttca aaacaagagt
     1501 tggaagagcc tcgccatcca catcatccgc aataatcaag agtggacgat tgctttggag
     1561 aatgctttcc aaaagtggca agatttcttg gatattggaa attttcttgt ctgtaatcaa
     1621 aatgtacgga ttttcaaggt cagccaccat tttttcgcta tcagtcacca tgtactgtga
     1681 aaggtaacca cggtcaaact gcattccttc tacgacttca agctctgttt ccataccacg
     1741 tgactcttcg atggtgatga caccgtcttt gccaactttt tccattgctt cagagatgta
     1801 ctcaccaact ttttcagaac gagaagatac ggctgcaact tgagcgatag cttctttatt
     1861 ggcaacaggg atggcgttgt ttttcaaagc ttcaactgct gcggcaactg ctgtttcaat
     1921 cccacgacga ataccgattg gatttgcacc tgctgtgacg tttttgattc cttcacggac
     1981 gatagcttgg gtcaagactg ttgcagtcgt agtcccgtca cctgcgatat cattggtttt
     2041 agaagctact tctgatacta acttagcacc catattttca aaatggtctt ccaattcgat
     2101 ttctttggca atggtcacac cgtcattggt aatcaagggt gaaccgaatg acttttcaag
     2161 aacgacattg cgaccttttg gtcccaaggt tactttaaca gtgtctgcaa gaatatcgac
     2221 accacgaacc atggctgaac gggcatctga tgaaaattta atttcttttg acatacttac
     2281 tttctccttc tattcctcaa tgattgccaa aatgttagct tcgcctacga taatgtactt
     2341 ttcatcgcca tctttgacat caagacctgc gtgggcttca actaagacac gatctccagt
     2401 tttaacactt ggagcaacca agtcaccgtt caaggtacga acaccttgtc cagtagccac
     2461 aacttgagct gttttggttt tttcttgggc tgagcctgca aggacaaagc ccccaacggt
     2521 ttgttctttt tcttctattt ttaagaccac acggtcccct aatggtttca acatctgatt
     2581 tcctccataa tgagatagat attattagca ctctttgaac tggagtgcta attcataatt
     2641 ctattgtatc acttggtcag aaatagtcaa gaaaaaagtc tgactttctc aagataaaaa
     2701 gcctgagacc aactcagact ttttaatgtt tatactcaat gaaaatcaaa gagcaaacta
     2761 ggaagctagc cgcaggctgc tcaaagcatt gctttgaggt tgtggataaa gctaacgtgg
     2821 tttgaagaga ttttcgaaga gtattaaaat ggcaattctt cctcttccaa gaccaaatct
     2881 gccaaatctt ggcctgcatt attttcacgc atggcacgtt gagcacgact ttctaagagt
     2941 tggaatcctg tcacaaggac ttcggttaca tagttcatct gaccattttt ctcaaagcga
     3001 cgggtacgca attctccatc aacggaaatg agactacctt tggttgcgta gcttgccaaa
     3061 gtttctgcta gtctgcccca taggaccata ttgacaaaat cagcttcacg ttcaccgttt
     3121 tggtctttgt aacgacggtt gacagcgata gttgctcgcg ctaccgactt gtcattgttg
     3181 gttttgtgca attctggtgt agacgttaaa cgcccaatca tgataacttt attatacata
     3241 ttttcttcct cctacttatc tattcgtagg aaatcaaaaa agttacagaa atttgtaact
     3301 tttcgaggaa atttcttatt ttttatgaat catgaaacct gtcgcctgtt gattggccat
     3361 aatggtcata tctgtaatct gaacacggcg aggctgactg gtcacataga ctactgtgtc
     3421 tgcaatatcc tgagcttgca aggcttctat tccttggtaa acggacgcag cccgctcttt
     3481 atcaccatga aaacgaacag ttgagaaatc tgtttcgaca atcccaggct gaatggttgt
     3541 caccttgata tccgttgcga tggtatcaat tcgcagtcca tccgaaaagg tcttaaccgc
     3601 tgccttggta gctgagtaaa cagcggcacc agcataggcg tagattcctg cggttgaccc
     3661 catattgata atatgacctt gattgacttt tatcattgct ggcaagaaac agtgagtaac
     3721 cgccatcaga cctttaacat tggtatccaa catggtcaac atatccaact cctcatagtc
     3781 ttgataggga gccaagtcaa gagccagtcc ggcgttgttg accaagatat caatcctccc
     3841 tatcgtttct agaatatcag agcaaacagt cttcaccatg accatatccg tcacatctag
     3901 tggaaaagtc cagacggttt gatttggaaa gcttactgca aactccgact tgaggatttc
     3961 taatctgtct atccgtcgtc ctgttagaac gacatcctca ccctgctcca gataagcacg
     4021 cgcaatcgct tcaccgattc ctgaggttgc tcccgtaatc actacatttt ttgccatctt
     4081 atttccttct atcagatgct gacaagttcc taggcagtcc agtgtttcac tgggtcgaac
     4141 ggtgttccga caacttggtc ttctgataat tcaagcaccc cacgtttttg cggagcattt
     4201 ggcaaatgca attcacgagg actacacatc atcccaaaac tcttttcacc acgaagttcg
     4261 cctgggaaaa tgaaattccc ttttggcatc atggcaccag gaagagccac aatggttttc
     4321 aacccaacac gcgcattagg tgctcctgca acgatttgca ctatcttgtc acttgcgact
     4381 gcaacttggc agatgttgag gtggtcacta tctggatggg ctaccatctc aacaatttca
     4441 ccaacaacaa acttaggttc cttgtcattg acaatttctt ctgtaaaacc ttccgcctgt
     4501 aattcttggt tcaaacaagc gacttgctca tctgataaaa agacttgacc tcgctctgca
     4561 atttcaaaca agctcgaaac ctcaaaaatg ttccaagcta ctgtttcccc attttctttg
     4621 aggaaaacac gggctacctt gcctttgcgt tccacattca gtttggcatc tccgctattt
     4681 ttcacgatga ccataaggac atcaccgaca tgttctttgt tatatgtaaa aatcattgtt
     4741 tactttttct cctatttcaa tcctgctaaa aaatcgttaa tttgctcctt acttttacgg
     4801 tcgcgattga caaaacggcc gatttccttg tccttttcta gaacaacaag gctaggaatt
     4861 ccgtaaacat cccagagttt ggccaaatcc atatactgat ctcggtccat tcgaataaag
     4921 gtgaactctg gattggtctc ctcaatctct ggtaaggcag gatagatata acggcaatcg
     4981 ccacaccagt ccgccacaaa aaggaagacc ttcttaccgt ctttttctac taaagatgcc
     5041 aattcttcta aacttgctgg ttgtatcata agacttcctc ctcatagact aggtcttcat
     5101 tttcatagac aaaagtataa tgacgaccat cctcaaaaat gacgccacca accaagctct
     5161 ccagactgct ttcgtaaact tgaacataga gggtcgcaat ttcccccatg tcggaaaaat
     5221 ggtctcgcac aatctctgtc aactcttcct gagtcttcat gagcttacgg tcatctgcaa
     5281 cttttttcgt ggcaagagca aggcttccga tacctagcag agccaagcct gccatccaca
     5341 tttttttagc tttcatacca ttcattttaa cacaaaaaag gcttcaggac aaatgaggaa
     5401 gcagcagaaa agcaagtaaa aagcctcttc ctttaaggaa aaggacttct tatactcaat
     5461 gaaaatcaaa gaccaaacta ggaagctagc cgcaggctgc tcaaagcact gctttgaggt
     5521 tgtagataga actgacgaag tcagctcaaa acactgtttt gaggttgtgg atgaagctga
     5581 cgtggtttga agagattttc aaagagtatt attcttattg ccaggcacct aagttgccaa
     5641 cgtagtaact atcaggtgtg taggtattgc gaagcatctt acctgatgaa gccagataat
     5701 actacttgcc attgtctttg acccaatcat tcgcaatcat ggaaccagaa gaacttacat
     5761 aataccattc tcccttgtca taaacccaag tactgacttt catggttcct gagcaattaa
     5821 aggcaaaaaa actgtccaat atcattcgtt ttttaaaagc atttgacact acatcatcta
     5881 cgctgtagta catagataca tcaaacgtaa aagccaaaat atcgaaaaat atacaacttt
     5941 taaaggcagc atttcatttt ttaaagtcta acaaccatag aactgaaatc agagcaaggc
     6001 atcaatttcc tactcttagt acgtctattt tgatggttga agtgaagatt tttttattta
     6061 acgtagacaa tttccaactt cccaaaagcc acatctccac ggataatcaa ggttttgctg
     6121 gttggggcta caggagcgtc agccttagct gcaccaaagg aggtttctac tctcaaatct
     6181 acacgccagt gttgtggaac atagacggtt gcatttccga aggccacttc aatatttaaa
     6241 gttgcaaaat cacctagcat ctctgcatta tcatagtaga ttttagcatc cccaaaagcg
     6301 acttccactt ggtcatctac gagatcttga tcttgcttat agaaggtccc gctaccaaaa
     6361 gcgacttcct tatccgtgac gactgttttt ttcccattgt accaccattt tttttcattc
     6421 cagaacttac ttgaatgcgt cagataacca acaccaagta ccaccaagat gctagcccaa
     6481 ataagagaat gattggtaac tggtaacaag tcataagcgt aatttgcaat gatgagcgcc
     6541 agtaaacctg taaaaactgc cgaagtgaga tgacgtctaa ggatggactc aatggactta
     6601 taagcaaaaa aaacaatacc tagtaaaggc catattttac catccaaaga aggaattcca
     6661 aaattccctt gcagcaagat ccaagctgct aaaaccaata aaacaatacc aaatgctttc
     6721 tttttcatgt tttttacctc atgtttctta gattttcttt taggagggat tggtaatgcc
     6781 gtgagacatg aacctccttg tgcgtctgat aaaaggaaat ggtgcccgtt cctgaaaagg
     6841 acttgtccac tgagtaaatc tgacggatgt ttgcgatcgt tgacttggaa actcgattaa
     6901 aatagcgagg caagatagac tccaactcat agagcttgag gcgaacctca taagcttcct
     6961 tctggttatg agcgtagatc ttgctccctt ctgtttcaaa gaagagaatt tctgacaggt
     7021 ctagataata ttcacctgtc cccttgtaaa aaatcaacct cggagccttt gactcttgta
     7081 agagccgttg caagtctgca atttcatctg tcaaagtggg ggtcttgatg acaatttcag
     7141 tctcctctaa attgccgtca atctcaattc gtaacttcat cacatctcct ttctgactct
     7201 actatatcaa agctaagtta ggaatacaag ggcaaaaaag caagtggtca atttgaagca
     7261 gtaagtggtt gtaggactag cttaacttac atgtcagaat aggaagaaaa ggaatcccca
     7321 caccaaacca caagcatagc caaccaagac atccttggga taatgaacac cccctaaaac
     7381 cctcaccaac cccagcaaag ctgaaaagat caagcatact aagccaatag gtagactagc
     7441 atgcagacag gccatggaga tgatagttgc cgaaaagaca tggcgactgg gcatcgaccg
     7501 tcccgaacta tccttatcaa gaaggggaca gatatcccaa gtttcataag gtctcgggtg
     7561 attgattttc ttacgaaaca gggacaaggc cacaaaacct gatgcaggaa taagcacata
     7621 aaccccaact tgctgaccaa gtcctagttg caggtaagtg gtgactagca agatcagata
     7681 gaccaggggc atggcgactg tcatcaaacg attgaaactg cgtaatagga ataaaagaat
     7741 tggacgacta gttagtctgg agctgatatt tcgataccat tcttgataat ttttcattta
     7801 tgcttctcat acttttctag aacttgttga gatgttttcc ccttggcaag ccaatccact
     7861 agcttgtcca agcgacgtat ttcctgcatc agaggatcat caatttcctc aatacggaca
     7921 ccgcaaatct tccctgtaat tgcaattcgt tcagggacat aatatggtgc ttgtctaaaa
     7981 aaatctccgt aagtaacatc cctatccaga caagcaagaa catcactcac ttcataccct
     8041 gtcaaccaac tggtcacttg gtgaactgac tctgcctttc caccctttcg ctcaactttt
     8101 gcaatcaagg ctaagtagac agcagcaaat ttcatattgt ataatttctg actcatactt
     8161 ctctctttct atgtgtacta gtataaataa gaaaaagaag gccgtcaagc cttcttttga
     8221 tttattcttc tgcttcatct tctgtaaatt gactattgta caagtcagcg tagaatccac
     8281 cttgcgccat cagttcctca tagttgcctt gctcgatgat atttccatct ttcatgacca
     8341 agatcaagtc tgcatttcgg atggttgaca agcggtgggc aatgacaaag gatgtgcgtc
     8401 cttccatcaa acggtccatg gctttttgga tcaattcctc tgtccgtgta tcaacagaag
     8461 aagtcgcctc atccaaaatc aaaagcggtg catccttaag aagggcacga gcaatagtca
     8521 atagttgttt ttgtcctaca gacaaggtca cggtgtcatc caagatggta tcatagccat
     8581 ctggcaaggt cataataaag tggtgaattc ccacagcctt actagcttcc atcattcgtt
     8641 catcactaat ccctatttga ttatagatga gattgtctcg aatagttcct ttaaagagcc
     8701 aggtatcctg caagaccatt gaaaaggcat catgcacttc tgaacgcgtc atagccttgg
     8761 tatccacacc atcaatgcga atacttccct tatcaatctc atagaatttc atcaaaagat
     8821 tgacaatggt tgtcttacca gccccagtcg gcccaacaat ggcaaccttt tgaccagcat
     8881 gagctgtcgc agagaagtca tagtcttgaa cattgacacc gtccaccaga atttctcctg
     8941 ctgacacgtc gtagaaacgt ggaatcagat tgaccagagt tgatttacca gaacctgttg
     9001 acccaataaa ggccactgtt tgaccagttt ctgctttaaa tctaacatgt tcaataactg
     9061 cctccgaatt tgccgcatag cggaaggtca catccttaaa ctcgacctga cctttgaagt
     9121 tttcatcagt cagctgcact tgaacagggt tttggataga agaatgcaaa tctaaaactt
     9181 gattaatccg cttagcagag accatagttc ggggaagaac aatgaagagt gctcccatga
     9241 gaaggaagcc catgacaacc tacatggcat aagacatgaa aacaatcatg tcactaaaga
     9301 gaggcagacg cgctatcgga gcagcgtcgt taatcacata ggccccaatc cagtaaatcg
     9361 ccacactcaa accacttgaa atccccatca tgataggatt caaaatagcc ataagacggt
     9421 tgacaaacaa attcaaacgg gtcaattcat catttactgc tgcaaatttt tcattttgat
     9481 aatcctctgc attgtaggca cgaacgacac gaatacctgt taaactctca cgagtgatac
     9541 tgttcagttt atctgtcagc ccctgaatca aggactgttt tggaaaggct agcgtcatca
     9601 aaacggtcgt catcaggacg ttgacaatca ctgccacaag tacggcccag agtcagtatt
     9661 ctgaatgacc taaaatcttc ccaatagccc aaatagccat aattgaacca cgtgttacca
     9721 cttgcaagcc catagtaatc aacatttgaa cttgagtaat gtcattggta gtacgcgtca
     9781 agaggctagg aattgaaaat ttcttaatct ctgtctgcga gtaatccaaa actcggttaa
     9841 aaatatcact tctcagccta ctagtataag aagccgccac tcgggatgca aaaaatccaa
     9901 ctgcaactac ggacaagaag gcaagaaaga gcattcccat catcatgctt gccggctgcc
     9961 acaactcatc taaattagtt tcttgactac ctagcaaatc cgtaattttc gagatatagg
    10021 tcggcacttc caactctaga tagaccgaaa agcaagtaaa gagaatggct agtaaaatca
    10081 tcccccattc ttttctacta attcttttgg ttaatttctt tattctctcc tcctattccc
    10141 ttgatatttt gcctgtagtt gaccgagaac tttctcaaaa atcagtaatt catcttcatc
    10201 aatgtcttcc atcaactgct tgtctatgcg ttcaaaaaaa gccttaacct gttgcatctg
    10261 agaacgtgct ttgtccgtca gacgaacaaa cttagcccgc ttatcaacag gactcgcctc
    10321 caattccacc aaaccatttt gcactatacg cttaaccaga ttactagcaa cagacttggt
    10381 aatattgagt tcctgctcga tatctttaat caagaccaag tcttggtttt tctagcgatt
    10441 atccaaaaaa cgcacaacct gaccttgcgg cccacccata aattcaatgc cgcaacgttt
    10501 ggcttccttt tgcaccatca ggtgaatttg atgaccaaaa cgcttaaaga ctaacatcgg
    10561 tttatccata atctccccct tctaaataaa aatagttctc tggagaataa ttaaatttct
    10621 atgagaacta ttttcttgat taaaaaaatc ccaagtgatt ttctcactta ggatcatgtt
    10681 ctataggtta aattaaaacc catctacgtt cgtataaatc ttttggacgt cttcgtcgtc
    10741 ttcaagaacg ctgtaaagtt tttcaaaggt ttcaaggtct tcgcctgaca attccacttc
    10801 tgactgagga atcatttcca attcagtcac ttggaattct tcaataccag actcacggag
    10861 agcaacgata gccttgtgaa ggtcagttgg cgctgtgtaa actgtgattg taccttcttg
    10921 tgcttctacg tcatccacat ccacatccgc ttcgagcaat tgctcaaaga ctgcgtctgc
    10981 atcttcacct ccaaatacaa taacaccttt gttgtcaaag aggtaagaaa cagaacctga
    11041 agcgcccatg tttccgccgt ttttaccaaa ggctgcacgg acattggctg ctgtacggtt
    11101 gacgttagaa gtcaaagtat ccacaattag catagagcca tttggcccaa aaccttcgta
    11161 acgtccttct gtaaaggttt cgtctgtgtt tcctttggct ttatcaatcg ctttatcgat
    11221 aatgtgtttt ggcacttggg cttgtttagc acggtcgata acgaatttca aagctgagtt
    11281 tgattctgga tctggatcac cttttttagc tgctacatag atttctacac caaattttgc
    11341 atatacctta gagttagctc catctttagc cgttttcttg gctacgatat tggcccattt
    11401 acgtcccatt aggaatctcc ttttttcaca ttttaatctt tattattata acacaagttt
    11461 ttttgatttt cactagagga aatggatttt attagcaaat caagctagga tagcacttta
    11521 cctgctaaga tggtcttacc tttctatctt tatcaacaga cactcatcca cattcaaaaa
    11581 acaaactaga ccattatctg caaatagaaa gtttcagcca agtttgacaa agtcagctca
    11641 aattactgtt tgaagtttgt agatataagc gacaaaaaca atcatactgc accttttgtt
    11701 gacagtctac tccagacata tcatagttca agtaaatact ttgaaattca acagttctta
    11761 taggcgctat tgtattctaa gaaatcaata gaagagtttc taagcaaacc tctaatactc
    11821 aataaaaatc aaagagcaaa ccagaaagct agccgcaggt tgctcaaaac actgttttga
    11881 ggttgcggat ggggctgaca tggtttgaag agattttcga agagtataat ttacgtgttc
    11941 ccaagatgga gaagttagac tagtacactg gcacttctaa accattgcta gcaattgatt
    12001 tgttcatatt taatttcatt ttttccataa atgggtatta gatataaaca gcaaaatatt
    12061 tccgatacgt gtcgttcttg aatttccaat catctaaaac aagtaaagga taatcaatcc
    12121 cctgtatatc aaggaattgg ctaccctttt tactttttta cacattctgt ttgatagatt
    12181 cattttaaca tcacgagcat actccaatgg aaatcgctag gcaagagata aactttcaga
    12241 tatccgcaga gagatcatcg cctctttttg tcgcaagcat tctcctctcc tagtcatttt
    12301 ctaccttatc ttctacctga ggatagagag ttgttcccca aatagaaatc gtccgcttac
    12361 gcactagtgg caaatcggtt ttttcataaa ccgtacgcca ccattcccag gcaagcccgg
    12421 tacactctct aattttgaca gagagattac gaacattccc ttttaaagga atactagtgg
    12481 taaagtgagc cgttaaatcc tgcccatttc tgtcccaagc cttaggagtc aagacttcct
    12541 taccttgatg atcataggat aattcattcc aagtaatata atattgggca acataggcac
    12601 cactatgatc cagcagtaaa tctccgtttc tgtaagctgt aaccttagtc tcaacatagt
    12661 ctgtactatt ttgaaaggtc gcaactacat tgtcacgtaa aaaagaagtt gtataggaaa
    12721 tcggcaagcc tggatgatct gctgtaaagc gactgccttc ttgaatcaag tcctctacca
    12781 tatccacctt gcctgttaca actcgggcac ccgaacttgg gtcgccccct aaaataaccg
    12841 ccttcacttc tgtattgtcc aaaatctgtt tccactctgt ctgaggagct accttgactc
    12901 cttttatcaa agcttcaaaa gcagcctcta cttcatcact cttactcgtg gtttccaact
    12961 tgagatagac ttggcgccca taagcaacac tcgaaatata gaccaaagga cgctctgcag
    13021 aaattcctct ctgttttaaa tcctctaccg ttacagtatc ttgaaacaca tctcctggat
    13081 ttttaacagc atctacgctg actgtataat aaatctgctt aaaattaaca atctgaatct
    13141 gcttttcgcc cgaatggaca gagttaaaat caatatcaag agaattccct gtcttttcaa
    13201 agtcagaacc aaacttgacc ttgagttgtt ccatgctgtg agccgtgatt ttttcatact
    13261 gcattctagc tgggacatta ttgacctgac cataatcttg atgccactta gccaacaaat
    13321 cgtttaccgc tccgcgaaca cttgaattgc tggggtcttc cacttggaga aagctatcgc
    13381 tacttgccaa accaggcaaa tcaatactat aagtcatcgg agcacgatcg accgcaagaa
    13441 gagtgggatt attctctaac aaggtctcat ccactacgag aagtgctcca ggatagaggc
    13501 gactgtcgtt ggtagctgtt acagaaatat cacttgtatt tgtcgacaag ctccgcttct
    13561 ttctttcgat aacaacaaac tcatcgggta gctgattacc ctctttgatg aaacgatttt
    13621 caatactttc tccctgatgg gtcaagagtt tctttttatc gtaattcata gctagtataa
    13681 agtcatttac tgctttattt gccatcttct acctcctaat aagttcctgg attgagttgc
    13741 ataaactcag acttgttcag cgaaatcagc cgtggttgga ctaagtaatc caaaatttcc
    13801 tcgtacaatt cttctgagac attgcgtcgc cgtctggcta gataagaagt cggaatgccc
    13861 gtattatcca acataaatac cttatctaag tcaatcaagg ttggtcttgt aaaaggatta
    13921 cgagctagat ccggctcttc tatcataaag ttcttgacca aacgtctggt caagagagct
    13981 ggtttgaagg tctgattttt aaccaactct ttgtttttag tcatgctgtt gtcaatacag
    14041 atatacatat gattcttcac agccaaatcg ctactaatag tcggaaaagg caaataaaga
    14101 gctacaacat ctcctctctt aatcaagcaa gagcaccccc ttttctccta atgtaacata
    14161 gacaggattg accaagtctt ctgattgact cagaatttcc aaagtttgag tttggcgcgc
    14221 tgtcaattta gtagcatctt gtctcttcaa tacaaaatgc ttgtcgccaa taaccttgac
    14281 aatataatcc ttctccaaag ctgactggta aatccacatc agatgttgtc tgtcctgaga
    14341 actcaagaga gaaggatttt caagcctccc gatagtctga taaaaatcaa aaacaggagc
    14401 taactcctgc caatctgatt ggctagttgt caaggctaga aaaagggctt tgcgagctga
    14461 tacttcttgg ttagccttga gagttacttt cccctccaag ttttttagaa atcgggaaac
    14521 tccagaaagc aaatttttct ctaactgcga gaaataaaaa cctttcgttc ccagacataa
    14581 gtctttcatg tcgctttctc tagcaaataa gagctcaaac atttgatagt aaaagaaaaa
    14641 tatctggcac tgggtcgcgc tcatcttttc cttatcggct tcttttttta accagagcaa
    14701 gggcgacagg tagctggatt gagacatttc ctctacctcc tactcttttt taactggagc
    14761 atctgcacta gctgccactt cttttgactg gatactttcc cactggttaa tctcctctga
    14821 gataagacct tcgcatgtct tgacaaatag ggcaaaagcc ttggtctttc ctgcatattt
    14881 ctccgtttgg cattgataga ggaatttttc tttctccagg agttgcgcag ttttttggta
    14941 agaaatccaa ttttcctttg cattatacaa attgataatc ccctcacaca gcaagccgag
    15001 actggataag gcaaccgaaa tcaaacggta gcgatcacct ggcataggaa tagcacaaaa
    15061 gacagctatg aggaaacctg ccacgatttc tgttattttt aataccttat agcgtctacg
    15121 atgttgaacg cttttcttta aaaaatgagc tatctgtacg tctaatcgct ctgtcaggta
    15181 catttcttct ggcgtcatat tcgtaactcc tttcatttac tttgataacc atagtataac
    15241 atatttgaat atcaaagtat agtaaaaact ggactataac t
//
LOCUS       DAVLSL010000036        15248 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLSL010000036 DAVLSL010000000
VERSION     DAVLSL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.036"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(231..1838)
                     /locus_tag="VXN30_000898"
     CDS             complement(231..1838)
                     /locus_tag="VXN30_000898"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1357549.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2228..2815)
                     /locus_tag="VXN30_000899"
     CDS             complement(2228..2815)
                     /locus_tag="VXN30_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1357550.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2943..3392)
                     /locus_tag="VXN30_000900"
     CDS             complement(2943..3392)
                     /locus_tag="VXN30_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357551.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3397..3786)
                     /locus_tag="VXN30_000901"
     CDS             complement(3397..3786)
                     /locus_tag="VXN30_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1357552.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3814..4440)
                     /locus_tag="VXN30_000902"
     CDS             complement(3814..4440)
                     /locus_tag="VXN30_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1357553.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVIFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVFASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4454..5017)
                     /locus_tag="VXN30_000903"
     CDS             complement(4454..5017)
                     /locus_tag="VXN30_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1357554.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5182..5416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5470..5973)
                     /locus_tag="VXN30_000904"
     CDS             complement(5470..5973)
                     /locus_tag="VXN30_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1357555.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6166..6996)
                     /locus_tag="VXN30_000905"
     CDS             complement(6166..6996)
                     /locus_tag="VXN30_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1357556.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6981..8663)
                     /locus_tag="VXN30_000906"
     CDS             complement(6981..8663)
                     /locus_tag="VXN30_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357557.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8765..9022)
                     /locus_tag="VXN30_000907"
     CDS             complement(8765..9022)
                     /locus_tag="VXN30_000907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357558.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9029..9577)
                     /locus_tag="VXN30_000908"
     CDS             complement(9029..9577)
                     /locus_tag="VXN30_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1357559.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9592..10440)
                     /locus_tag="VXN30_000909"
     CDS             complement(9592..10440)
                     /locus_tag="VXN30_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1357560.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10629..11978
                     /gene="trkA"
                     /locus_tag="VXN30_000910"
     CDS             10629..11978
                     /gene="trkA"
                     /locus_tag="VXN30_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1357561.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11982..13421
                     /locus_tag="VXN30_000911"
     CDS             11982..13421
                     /locus_tag="VXN30_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1357562.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13546..13803)
                     /locus_tag="VXN30_000912"
     CDS             complement(13546..13803)
                     /locus_tag="VXN30_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEV1357563.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            14563..14733
                     /locus_tag="VXN30_000913"
     CDS             14563..14733
                     /locus_tag="VXN30_000913"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1357564.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
BASE COUNT         4567 a         3419 c         2753 g         4509 t
ORIGIN      
        1 atccagttcc taaaactttg ctagaaattg atttggcttt ccgaattaat ttgttcagat
       61 tttatttcat ttcaatatac tgataaatcc attcactagc acagggcaag agggagtcgc
      121 tattaatttc aacggtagca ggtacataca gaggactcct acctcagata tactgcaata
      181 tcattgtatg caaaaaacct ctgagatttt tctcaaaggt tctgattttg ctaattgctg
      241 ttctcaaccg ctgcagtaac aaaggcagtg tagagttctt ctggtcggtt tggacggctt
      301 gacagttcag ggtgatactg acaagctaca aagaatttat tttcaggaat ttccacgatt
      361 tctaccaaac gattgtctgg agaaactcct gaaaagacaa aacctgctgc ctcaaactgc
      421 tcacggaagg cattattaaa ctcataacga tgacggtgac ggcgttgcac cacttcttga
      481 ttgtgataag cagcagcagc cttagagcca cgtttcaact tagacggata aagtcccaaa
      541 cgaagggttc cacccatatc ctcaatatca atctgatcac gcatgatatc aatgataggg
      601 tattttgttt ctggtgcaag ctctgcagaa ttggcacctt caagacctaa aacgtgacga
      661 gcaaactcga tacatgtcaa ctgcattccc aagcagactc ccaacattgg aacatcattt
      721 tcacgcgcat agcggatggc ttggattttc ccttctgtac cacgttgacc aaaaccacct
      781 ggtacgatga tcccgtccgc atcagacaag agttctgcta cattctctgc tgtcacatca
      841 ttggcattga cccaattgat tttaacttct acatcattga catagccaga gtgtttcaag
      901 gcttcgacca ctgagatata ggcatcttgc aactccacat acttaccaac aagggaaatc
      961 ttaacttgct tcttgaggtt catgaccttg tccaccatgg ctgaccattc tgtcatagcc
     1021 gctgctggtg cgtctaattt caaatgatca caaacaattt ggtccatccc ttgtgcctgc
     1081 aagttcagtg gaatttggta aaggtgttca acatccaacg attcgataac ggcttctggt
     1141 gccacatcac agaactgggc cagtttattt ttaattcctt gaccagctgg ctcttctgta
     1201 cgaataacca acatatttgg ttggattccc aagccacgca attctttgac agagtgttgg
     1261 gttggtttgg ttttcatttc accagcagcc ttgaggtaag gaagcaaggt tgtatggata
     1321 tacatgacat tatccgcacc cacatctgcc ttcatctgac gaagagcctc taggaatggc
     1381 aaggactcga tatctcctac tgttccacca acctctgtga taatgacatc agagtcggtc
     1441 gttagagcgg cacgcttgat tttttctttc aaagcatctg tgatatgagg aatgacttga
     1501 acagttgccc caaggtattc tccacggcgt tctttacgaa gaacttcact gtaaattttc
     1561 ccagttgtca cgttggaata tttgttgaga ttgatatcga tgaaacgttc atagtgaccc
     1621 aagtccaaat ctgtctcagc tccgtcatct gtcacaaaaa cttccccgtg ctggtaagga
     1681 ctcatggttc ccggatcaat attgatataa gggtcaaact tttgaatggt tactttgaga
     1741 ccacgatttt tcaagagacg gcctagactc gctgccacaa tccctttccc aatagacgat
     1801 accacaccac cagttacaaa aatatatttc gtagacatag attcctcttt ctaaaatgct
     1861 caaggtcttg ttaactacga ggggatatag aaaacaagac cctactaata agaataatct
     1921 gggacgactg caccagattc acaactaata ctcttcgaaa atctcttcaa accgcgtcaa
     1981 cgtcgccttg ccgtagatat gtgttcctga ctttgtcagt cttatctaca acctcaaaac
     2041 agtgttttga gcagcctacg gctagtttcc tagcttgctc tttgattttc attgagtata
     2101 aaatacaaga agataacttc ttgaaaaaac aaaaatagct ccctaagaac tagggagccc
     2161 cgacctctaa aagaggtgcc caaacaatat gatacctaaa aataggataa ttgtcaatag
     2221 ctaactctta ttcctcgctg gtttcagcgt cgtcatctga aaactcgtcg tcgtcttcgt
     2281 tgagatccac atcttctccc aagtcatctg gagcgatttc gttgatttct gcatcataag
     2341 cttccacttc atttttttca tcatctggat tttcatcatc gtatgaaaga gctggatctg
     2401 cttcgtatgc atcttcgtct tctggatcat ctgcattgta gtcaatggca tctgaatcac
     2461 catccataaa ggcattgaca cgttttttct tagcttttgg tgctacttca tcgtcgtcat
     2521 tttcttcaag agcgatgatt tcttcgtcga tttcgtccac accataccat gaacgaagac
     2581 cccatttgtt gtccccaagt gagatgaagc taccgtcaaa gttcaactct gtgtagaaca
     2641 aaggcaaagc ttcgcggata tcgctgtttg atgttccaag gtagttttga atttcgttta
     2701 caagatcgct aaaatgcatc tcatgatcgc gaccacgaag ttctaagata gcacgcgcta
     2761 cctcaatcat agatagttca cttttttctt gcccagcaaa tacttctaat tccaaagcgt
     2821 ttctcctcat ttatactact atcgtcagag cgaacagact ctgacctcat tttatcattt
     2881 actctttatt ttacgataat tttgcggaat agtcaaaggt taagggggag aaagtggcag
     2941 gattagacta attccaatat aaaactcctt cctttttctg ttgctccatt ttccacaaat
     3001 ccaagcgact tgaaacacct cctagaagca tgattgtagg tgtagatttc cttgactctc
     3061 aattctttcc atccttttac tcgagccaat tcaatcaaag cacttagaat cttttttcca
     3121 agtcctcgat gttggtaagc ggaattccca atcacaatgg ggagattatc ctgagatagt
     3181 gtaatatccc caattggaaa ccattctccc ttctccttga cttcaatcca aaaaagctca
     3241 ccatgccgat ccaaatagga atacatggct tccaaggtcg cttgactgta aggaagcttc
     3301 accccatcta cgaggtaaac caagttcaca tcctgatacc aagcaaaagc ttcctcacaa
     3361 tgattgacct tataataagg aacaaaacac aatgcactac cttttcaaca aaagagttgc
     3421 tgcttgatta aaaccatcac accagttata ccattttgct tcatacccat cttgagctag
     3481 gatacgatct tctaaatcaa aaacagagta aatctttctt tcctcgcaag cttgcgcata
     3541 gagatgatat agttcatcac caccatctct atcccactca gcagaaatcg tatcccgacc
     3601 tgccaataaa gcctgataag ccctgtgatg cccatctgta atcagcaaac aatctccaaa
     3661 ggcaagaata ctgattggat cgacttggtt tgtttctgcc gactggtaaa gcatctgaat
     3721 atcttgcaac ttcttttctg ataaatatag ttgagtcgga tgaagatctg ctatattgac
     3781 tttcatttct ttctcctcaa gggaattcga tactcacttc tgtttgcctt taaatcgcca
     3841 ttggaagcgg agcttgtcat agaagggaaa ctcgataaac aggactccca agcccacaca
     3901 gagactggca aagacgtctg atgggtaatg aacccccaga tagactcttg ataccagcac
     3961 actgactagg tagaggccaa ggacgatttg tacgattttt ctccagactg gatttttaat
     4021 tcgttgactg agaatgacaa tcagagtacc taccatcaaa gttacagcca gagaatgccc
     4081 acttgggaag gaaaatccct tctcttccac cagatgtaag atagctggtc gtgggcgctg
     4141 gtagatattt ttaaagatca cgattaaaag acctgccaaa gccagatttc ccagcatgaa
     4201 gaaactttct atcttccatc gcttacgata aaagacaaaa gctgtaatga caacccaagt
     4261 gataatcact gggatatcaa tcagacgtgt gagggctcga aaaagaatag tcaaataatc
     4321 tggtaagtct cctcgaatgg cagtctgaat cgattggtca aaattgacca acatttcagg
     4381 gtaaaatttg accatatagc caagaataac gaaaagtaaa agggcaaaac tgcccttcat
     4441 taaaaatgtt tgtttatctt tcatagtgtt ttaaggttgg tttcaaaagg acgtacaaca
     4501 accagaatga aacggaaaag attacaccct caatcaagtt aaaaggtaat accatggtca
     4561 ttaggtagtt ggaaagtccc aaaatttttc caatatcaaa gttagcaaac ttagcgtaca
     4621 aaggaacagc ataaacatag ttgagaacca acatggccaa ggttaaacca acagttccag
     4681 ctagagagcc tagtaggaaa cgaagggttg tccgttcctt tttccaaatc aaagcaaata
     4741 cgatgacaaa aactcccaaa gctacgatat tcatcggcaa accaatgtaa gtattcactc
     4801 cttggctgtt aagaagcaat ttcaatagtg agcgaagcaa gagcactcct agagcagcag
     4861 gcaaatccat gaccaccaga cccacaagga ctggcaagat actaaattcg atcttgagga
     4921 aagatgccgc tggtaaaagc ggaaagtcaa agtacatcag cacaaatgag atggctgata
     4981 gaattgcaat ggtcgaaagt cgacgtgtgt ttgtcataac aggttcctcc aattttctat
     5041 aaaatcagaa gaagttggaa aggattcctc tatctattct cactttttat atcccaaaag
     5101 ttccctctta ttctattaaa gaaaaacaaa gcaagtggtt acaatccggc tataaatcta
     5161 tcaaaacaga caaagctatt ctttcgtctt ctcccatcca gactatactg tcggttgtgg
     5221 aatctcacca catcagcttg cgctcgcgga cttctttaaa gagaaggaaa tagagatttt
     5281 tcttttagac taggcgacaa gctgtaggca taacttctgg ttaggcaaag cttgtcaaca
     5341 aagtataaaa ggaaaagatc atttcctagt taaagtcacc gccggtcggg aatcacaccc
     5401 tgccctgaag actcttttat cataacaaaa aacgcttgca agcgcaagca ttttgttcat
     5461 tttcattttt tatttcaatt tatccacttc ataggtatgg acgagctcaa gacctgcaaa
     5521 gttctgctgg cgaagagcct catagacaat catgcagacg gtattagaca cattgagact
     5581 gcggacatgt tcatcattca tgggaatacg gagagccttc tcaggatgtt ctcgcataaa
     5641 atcttcaggt aagcccttgt cttcacgtcc aaagagaaaa taatggtcct cgtcagtcga
     5701 taaatccacc tcagaataga ctttttccgc gaatttcgaa atcagataga gttttccctt
     5761 catctgagac atgaaatctt ccaaactctc gtaaaaataa atctctagct tatcccagta
     5821 atccaatcca gcccgcttca tcttgcggtc atcaatagga aagcccatag gcttgatgat
     5881 gtggagggga gaattggtcg cagcgcaagt acgcgcgata ttgcctgtat tttgtggaat
     5941 ttgaggttca aataatacaa tgtgatttgt catgacttgc ttcctttcac cattgcaaaa
     6001 aaatagccac actgcccgga gtcaagctca gcaaacagcg tggttaaggc atcgttaact
     6061 tacctcacaa caggtttgaa gtaaatcagc gaaactactt tcttagtata acactttcag
     6121 aatcattgtc aatagaaatg acttgatttt ttcaattttt tcaagctatt tccaagggtt
     6181 gtaaaatcgt ccctgattct gcaagataag tagtaaacta actactaaaa acaaggttgc
     6241 caagagcaag gtaatatagt ctcctttttt caaggcctga taactatacc atgtgcgttt
     6301 tttctctttc ccaaagcggc gaagctccat ggcagtcgca atagtatcaa tgcgttctag
     6361 cgagctaaaa atcaagggcg taataatgag cagattgcct ttgattcgtt gcataagaga
     6421 agctttcttg gataattcca tcccacgcgc ctcctgagac atcttgatag taaagaattc
     6481 ttcctgcaaa tctggaatat agcgcaaggt caggctgaca gaataagcaa tcttataggg
     6541 cacaccaatt tgatttaaac tggaagcaaa ctgactagga tgggttgtca tcaaaaagat
     6601 aatagccaga ggaatggtgc aaagatactt aatggccaaa tttagcagat aaaagagctc
     6661 ctggctggtt agagtgtaga caccgattcc ctgccaaatc acacttctct ctccataaag
     6721 tccaacccca tactcgggag aaaagagata gaccatcaaa acgtttaaaa cggcaaatac
     6781 cgtcgcaaaa acggctacaa aggaaacatc tttaaagcga atttctgata aatagaggag
     6841 aaagactgaa aagatggcaa tcagcaagag cattctggtg tcatagctaa tcatggccgc
     6901 caatgatacc agaatgaaaa agagaagttt cccagctcct gacaagcgat gaatcacagt
     6961 atctctatgc tggtaaccga ttaatttagc ttgcatccct ctctcctttc tttgtaaaat
     7021 gccgttaaat ccagtggatc cacatctagt ttcttagcca agttaaagat ggaggtttct
     7081 tttagattgg cttttactaa cagctcagga tcgctcaaca gactggctgg aacagtatcg
     7141 gcaatcaatt ctccatccac catgacaagg acccggtctg aataatccag catcaattgc
     7201 atatcatggg taatcatgac aatggtatgc cctttttgat gtaactcttc gagaaattcc
     7261 ataatctcag tatagttctt ctgatcttga cctgcagtcg gttcatctag gagaataatt
     7321 tcagctccta agaccaaaat tgaagcaatg gtgacacgtt ttttctgacc aaatgacagg
     7381 gcagaaatag gccaattacg gaattcataa agtccacaga ttttcaaggt ttcatagact
     7441 ctcgtttcaa tttccttctc atccacacct cgcaaacgga gccctagagc cacctcatca
     7501 aaaatcatat tggttgaaat catttgatta ggattttgta gcacatagcc tactcgttcc
     7561 gcccgctctg caacagaatc gccttttata tcctgttttt cccaaagata gcgtccttcc
     7621 gtctgaataa agctacttat agccttggct agagttgatt tccctgctcc attttttccg
     7681 acaatagcaa tcttttcacc ctttttaata tctaaatgta gggattttaa aatcggtcta
     7741 tcatcataag aaaaagatac ttcctctagt ctaaagagtg actgcaatgc tggggtttct
     7801 tttaccagtt ccgtctgtaa ctggacttgc ccttttgaga tagacaagtt atccagatct
     7861 gctaattgtt cttccttgac taagtccaca cctaattgac ggagagtcgt tagataaagg
     7921 ggttctcgaa ttccattttg agtcaataaa tcagtcgcaa gcaactggtc agggctccca
     7981 ttaaaaagga tacgaccatc gtttatcaag acaatccgat ccacagggcg atgcagaacg
     8041 tcctccaaac ggtgctcgat aatcagagtc gtcgtcccct cttccttatg aatctggtca
     8101 atcaattcga taatatcctg acctgacttg ggatctagat tggcgagtgg ctcatcaaac
     8161 aagagaatcg gactttcatc aatcaagaca ccagccagac tgactcgctg cttttgtcca
     8221 cctgacaaat cctgaggacg ctgatccagt aaaggaagaa ggtccagctt ttcagcccat
     8281 ttataaacac gacctttcat ctcatctagg gctgtcacat cattttccag agcaaacgcc
     8341 aaatcttctg ccacagacaa gccaataaac tgcccatctg tatcctgcaa aactgtgcta
     8401 accagatgag acttatcata gatgctcata tcaaaggctg tttgaccctt tatcaaaaat
     8461 tctccatatg tctgaccctt gtaaatattg ggaataatcc cattcaaaca ctgacccaag
     8521 gtagatttac ctgatccaga tggtccaaca attaagactt tttctccctt gtaaatggtc
     8581 aagtctatcc cttgcaaggt cggttcttgt tgtgtttcat accggaaaga gaaatccttc
     8641 cactcaatta tagcttcttt catcttactc tcttcattcg cttcttagac ttctatttta
     8701 tcataaatca agcccttctt gcagtctctt ctcttaaaat cttagcgcca aaaagattcc
     8761 tatcttagct tacttgccta actaatctat aaacatcgaa aaagactagt tgcccagcct
     8821 tccccatcat tttatactct tcgaaaatct cttcaaacca cgtcagcttc gccttgccgt
     8881 aggtatggtt actgacttcg tcagtttcat ctacaacctc aaaaccatgt tttgagctga
     8941 cttcgtcagt tctatccaca atctcaaaac actgttttga gcaacctgcg gctaacttcc
     9001 tagtttgctc tttgattttc attgagtatt agtccttttt caaacttcct gcacgagttt
     9061 gagttcctgc ataggcaagt aagagaagag ttcctgcaat agctacagat acaccattgg
     9121 caattcccgc aacaatccct tgtgcaaata ctttttctgc cgcttcttga taaatcacaa
     9181 catctccaag tggtgccaag acaccccaaa caagggcatt tgcaagtagt tgaatgaggt
     9241 taaaaataag aatatctttc cagtcaaaaa caccattgat cactcgaacg tactttctaa
     9301 aaagtcccac aactaaacca aagagtccgc tagcgataat ccaagtccac catagaccat
     9361 aaccagcaag agagtccttg attgcatgac caatcaaccc gacaagcaaa ccgataatcg
     9421 gtccaaaaat aatagaaagt agcgcttgta ccgcatactg aagctggatg cttgtatttg
     9481 gaacaggggt tggaatgttg atcatcccga tgacgacaaa gagggcagcg ccaattccga
     9541 cagcaacaac ttgtttaatt gtaaatttga tttccatact attctcctat tttatccttc
     9601 tattttcttt atttcaatgg tccaagatga accgacacct acattatagg ccttggcaaa
     9661 ggaaccttgg ttgatagcca aacctaaacg atagagagag ttgatgtaaa ggatgggttg
     9721 cccaattctc acatctgcaa atgatttgcc atagacaacc tgattttgat agaccagcat
     9781 atcagcatga tagatggtca cttcaaaacg atcaccaaat tctggttcca gcttgtaaaa
     9841 ttcttcccgt gtgatagagg tccaaagcga accgaaacgc acatccagaa tatcaatggc
     9901 tcccttcacc agatgatctt ctatgatggt cgctacgact ggaagctcta caatctgttc
     9961 cacactgagc tctggcccta cttcctcaaa agtaatgtga ccactggcca gtttagcacc
    10021 agtataggca tagacatcac gaccgtggaa ggtataagaa tgctctgtgt tttgacgcct
    10081 attggccacc tcagaaatct cacgaatggc tacaatgcca acgtgtttct tgataaagga
    10141 aagcgtccca ttatctggcg tgacaatgta ttgatttttt gcagtcttgg caactacact
    10201 cttacgtttc gaaccgacac ctggatcgac aaccgataca aacgtcgttc cctcaggcca
    10261 gtaatccacc gtctgaaaga gacgatagct cccctcgaaa atattataag gcgtgatatc
    10321 gtgcgtcaag tgatggattt ttaaggttgg agactcttct aaagccactc caatcatagc
    10381 cgataccgca ccatcaacca aaccaaagtc tgattgtaat accagtaaat tattattcat
    10441 tttatctcct tgtatatcct ttatcatacc atatttcaga gaaagtcgct aatagctatt
    10501 tcaattggtt agggtatagt tttaccttat agcaaatttc ctactaaaag ttcttgttat
    10561 tagctagtag gtgcattttt tcttgaaaaa gggtatgata gttacatcat gaaaaagtag
    10621 gttttattat gaaaattatc cttgtcggag ggggaaaagt tggttttgcc ctctgtcgct
    10681 ccttggttgc agaaaagcat gatgttttgc tgattgagca agacgaagct gttctcaatc
    10741 atattgtcag tcgctttgat atcattggta tccttggtaa cggggccgat tttgccattc
    10801 ttgagcaagc cagcgtccaa gattgtgata tctttatcgc cctgactgag cacgatgaag
    10861 tcaacatgat tgcagcagtt ctagccaaga aaatgggagc taaagaaact atcgttcggg
    10921 tgcggaaccc tgaatattct aactcttatt tcaaggaaaa aaatattctc ggtttttctc
    10981 ttatcgttaa tcctgagctc ttggctgccc gcgctatcgc gaatatcatt gacttcccca
    11041 acgccctgtc tgtcgaacgc tttgctggtg gacgcgttag cctcatggaa tttgtcgtca
    11101 agtccaccag cggtctttgc caaatgccca tttctgattt tcgtaaaaaa tttggtaatg
    11161 tcattgtctg tgcgatagag agggatcatc aaattatcat tccaagcggt gacatgactg
    11221 tacaggataa agatagaatc tttgtcactg gtaaccgtgt cgatatgata ctcttccata
    11281 attattttaa atcacgcgcc gtgaagagcc ttctcatcgt tggggcaggt agaattgcct
    11341 attatctact tggtattctc aaagatagtc gtatcgatac aaaagtcatt gaaatcaatc
    11401 ctgaaatcgc cagcttcttt agcgagaaat tcccaaatct ctacatcgtt caaggagatg
    11461 gaaccgcaaa agatatcctg ctggaagaaa gtgctcaaca ctatgatgcc gttgcgactc
    11521 taacaggagt cgatgaggaa aatctgatta cttcgatgtt ccttgacagg gtaggtgtac
    11581 agaaaaatat taccaaggtc aatcgtacca gtctcctcga aattatcaat gcgcctgatt
    11641 tttcaagtat catcacacct aaaagtattg ccgtggacac gattatgcac tttattcgtg
    11701 gtcgtgttaa tgcccagtat tcagaccttc aggccatgca ccatctagcc aatggtcaaa
    11761 tcgaaaccct gcaattccat atcaaggaag ccaataaaat gactgccaaa cctctttctc
    11821 aactgaaatt aaaaaaaggt gttcttattg cagccatcat tcgaaagggc aagactattt
    11881 tccctactgg ggaggatatg ttggaagtcg gagacaagct cctagtaaca accttgttgc
    11941 caaacatcac caagatttat gacttgatcg cgaggtaaga aatgaataaa agtatgattc
    12001 gttacctcct ttcaaaatta cttttgattg aagctgttct tcttttggtt cctgttgcga
    12061 ttgctgtcta ttaccgtgaa tcgagccaag tctttacagc cctcttttcg accataggga
    12121 ttctcgtatt gttaggcggt tcagggagtt tacagaagcc aaaaaatcaa cggatttatg
    12181 ccaaggaggg agtcttgatc gttgccctct gttggatcct ttggtctttc tttggtggtc
    12241 tcccctttgt cttttctggg caaatcccta gccttattga tgcctttttt gaaattagtt
    12301 ctgggtttac aactactgga gcaagtattt tgaacgacgt ttcggttctc agccgttccc
    12361 tcctcttctg gcgaagtttt acccacttga ttggagggat gggagtgctt gtttttgcac
    12421 ttgctattat ggacaatgcc aaaaatagcc acctagaggt gatgaaggct gaggttccag
    12481 gtcctgtttt tggcaaggtt gtatccaaac taaaaaacac tgcccagatt ctctatctcc
    12541 tttatctagc tctcttctcc ctctttgtca tcatctatta tctagctggt atgcctctct
    12601 acgatagttt tgtcattgct atggggacag cgggaactgg aggctttacc gtctataacg
    12661 acggaattgc ccactatggc agctcactga ttacctatct ggtcagtatc ggagttctgg
    12721 tttttggggt aaatttcaac ctctactact acctcatgct ccgtcgcatc aaggccttct
    12781 ttggtgacga agaacttcgg gcttacttgg tcattgtact ggtttctaca ggcttgatta
    12841 gcctcaacac cctctacctc tacccagggt tttcaaagag ctttgaaatg accttcttcc
    12901 aggtttccaa catcattaca acaactggtt ttggatatgg agatattacc aactggcccc
    12961 tcttctccca gtttatcctc cttttcctca tggcaatcgg tggctctgct ggatcaactg
    13021 caggtggact caagattatt cgaggcctca tcctttcaaa aattgccaaa aaccaaattt
    13081 tgtcaatcct atcgccccac cgtgttttga ctctccatgt gaatcaaacg gtgattgata
    13141 aagataccca gcataagatt ctcaagtact ttgtcatcta tgctatgatt ttgctagcgc
    13201 ttatctttat tgtcagccta gatagcaatg attttctggt cgtgaccagc gctgtcttta
    13261 gctgtttcaa taatatcggg cctattctag gaaccacttc tagtttctca atctttagtc
    13321 ccatctcaaa aattctcctc tcctttgcaa tgattgcagg gcgcttggag atttacccaa
    13381 tcctccttct ctttatgaaa agaacttggt ctaagagata aaatacaaaa tccctctttt
    13441 tacaattgtg aaagagggat ttttatatta aaaaggggga acgtttttaa atccaagcct
    13501 gtctgtaagc aaagttctta ctcaaataaa agatggaaaa acttactaca cagcttggta
    13561 atgctccaat acaaattgta aggctttact tggattcttt gttaaatgaa tatattccat
    13621 tcctctggta gcaactatct gaatacctaa tctttctgca tccaccttca tctctctata
    13681 ataactccga acttgtggag ccagaataat taaatcataa acacccataa tatcataatg
    13741 agctccgtac gctcctgaat tcgcaatcac tctaacttct gctaattgag cgccctcgtt
    13801 aattgcattg gctaattacg cacttgttcc agaccctgca caaagaacca gtactttcaa
    13861 ctccttactt tttattacat ctatctcacc aggcattatt tcacttgtat cctcctctaa
    13921 aataatatca tttgagcttg caatatctac aacctgtagt agatatctcg tatttagaca
    13981 atatgaaaac aagacgactt ccatatagga aaccgccttc tctctatgtt gagtgattta
    14041 tattaaaata acttttcttc tagctgcatt ttattattat aaaaacattc atcataaccc
    14101 tgaacttaaa taacgatttt tattcaagat acatactcct agaataaact ttatatgaaa
    14161 ttctcatttt tgtttttaca attctcctta gttaaatctt gtttaatata tgttttacat
    14221 atagtattta gcgccacata gtactgaact ctctccaaaa acggttattc ctctttgaat
    14281 agggcgttat cacaagaaaa gcatctccac gtttcaactt catatggctc aaaaacaatc
    14341 aattgatgct aaaacctgta cctagatgtt tcggttcata aaaccatgaa actgtaaaag
    14401 tggatgaaat tgatagcgat agtcaaatca agaggcatca taactctaaa aagtcacaat
    14461 atataagttc atcctcggaa aaatatcatt ctaattgttg aaatgcctac atgaaaagaa
    14521 acgtcaaatg ctcatgaaac aacgaataca ggtatcaaaa ctatgacaaa acaagtccct
    14581 aaatttacta aagacactgc tcaactttac acctgtaaat ggttgttgta taataaagtt
    14641 acaaagatgt acgaccacac tgttgtaaat catagtgttc gcgaatatat tactgatagc
    14701 atttctacaa atacaattga agagactgga tagactttaa acgaaatatg tcagtgtttt
    14761 ggcattccta gccttcatat catttaaaga attctataga caaaattttt cccaatacag
    14821 acactcgtaa caactgcttc atttttctac caacatattt aggaacagga taagatacaa
    14881 gaggattaat ccatagatca gttctatacc aatctaagac aaataagcta aaaaaacgat
    14941 tgataataag caaatagatt ccaaattttc tctatctgct cattttaata aacaatacta
    15001 gtgtaactat ccttccagtc agaagcttgt caaatcacac cgaaaattct tctaaaattt
    15061 atctcgttag gcaatcaagc aaaaactcga cgatagtaca aacattatca tacagaattg
    15121 acttcctaaa ttatatactt tagtaaggtt ttcgtataag aaaaaagatt cattttacat
    15181 ttctaaacat tcttttctaa gatgaaaaac agaatttttc gattgtgatt taaagcaaca
    15241 agaagatt
//
LOCUS       DAVLSL010000037        14499 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLSL010000037 DAVLSL010000000
VERSION     DAVLSL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14499
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.038"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            276..2063
                     /locus_tag="VXN30_000914"
     CDS             276..2063
                     /locus_tag="VXN30_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1357565.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2060..2536
                     /locus_tag="VXN30_000915"
     CDS             2060..2536
                     /locus_tag="VXN30_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1357566.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2564..3469
                     /locus_tag="VXN30_000916"
     CDS             2564..3469
                     /locus_tag="VXN30_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1357567.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3456..4271
                     /locus_tag="VXN30_000917"
     CDS             3456..4271
                     /locus_tag="VXN30_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1357568.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4278..4682
                     /locus_tag="VXN30_000918"
     CDS             4278..4682
                     /locus_tag="VXN30_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1357569.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4981..6147
                     /locus_tag="VXN30_000919"
     CDS             4981..6147
                     /locus_tag="VXN30_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1357570.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6263..7300
                     /locus_tag="VXN30_000920"
     CDS             6263..7300
                     /locus_tag="VXN30_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1357571.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFAFPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7507..8361
                     /locus_tag="VXN30_000921"
     CDS             7507..8361
                     /locus_tag="VXN30_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1357572.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGIGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     RGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSKLEITDSDGI
                     VTRIRLAR"
     gene            8511..8747
                     /locus_tag="VXN30_000922"
     CDS             8511..8747
                     /locus_tag="VXN30_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1357573.1"
                     /translation="MTNSKYITRLKRSEGQLRGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAI"
     gene            complement(8981..9064)
                     /locus_tag="VXN30_000923"
                     /pseudo
     CDS             complement(8981..9064)
                     /locus_tag="VXN30_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(9098..9190)
                     /locus_tag="VXN30_000924"
                     /pseudo
     CDS             complement(9098..9190)
                     /locus_tag="VXN30_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9226..9522)
                     /locus_tag="VXN30_000925"
     CDS             complement(9226..9522)
                     /locus_tag="VXN30_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1357574.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNTWPGQVE"
     gene            complement(9512..9673)
                     /locus_tag="VXN30_000926"
     CDS             complement(9512..9673)
                     /locus_tag="VXN30_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1357575.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            9743..10507
                     /locus_tag="VXN30_000927"
     CDS             9743..10507
                     /locus_tag="VXN30_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1357576.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            11120..12499
                     /locus_tag="VXN30_000928"
     CDS             11120..12499
                     /locus_tag="VXN30_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1357577.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12513..13178
                     /locus_tag="VXN30_000929"
     CDS             12513..13178
                     /locus_tag="VXN30_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1357578.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13488..14026
                     /locus_tag="VXN30_000930"
                     /pseudo
     CDS             13488..14026
                     /locus_tag="VXN30_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            14126..>14499
                     /locus_tag="VXN30_000931"
     CDS             14126..>14499
                     /locus_tag="VXN30_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1357579.1"
                     /translation="MIRREDQSVNEQVMIKCIDQQLKVNRLLVDQLDLSQVSHPKMRE
                     YLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAIRKTMLSRLTKH
                     SVLPDRKLSNVVYQITKSVYGF"
BASE COUNT         3994 a         2745 c         3328 g         4432 t
ORIGIN      
        1 aaaaaattag aataatagtt tgaaaaatta agcgatatag ggattgctca tattttatca
       61 atgtattcta tacaaaatta ttttaggtcc tatattccga atttaaagag cgtttgaaat
      121 gggtctaaaa atgtttgaaa ctaattgaat gtgtgtataa tagtaaaaat gcagtaaata
      181 tcccgacaat ttctattgac aattcaaaca gattggttta taattaatat aacaacaaat
      241 gaaagcgcaa actttcgcgg tcggaaggta gttttatgac acgatttgag atacgagatg
      301 atttctatct cgatggaaaa tcatttaaga ttttatctgg tgccattcat tattttaggg
      361 ttcctccaga ggattggtat cattcgctct ataacttgaa ggctcttggt tttaatacgg
      421 tagagactta tgttgcttgg aatttacacg agcctcgtga aggtgagttt cattttgaag
      481 gtgatctgga tttagagaaa tttctccaaa tagcgcagga tttgggtctc tacgcaattg
      541 tgcgtccgtc tccatttatc tgtgcggaat gggaattcgg tggcttacca gcttggctct
      601 tgaccaagaa catgcgaatt cgctcatccg acccagcata tatcgaggca gttggtcgct
      661 actatgatca gttattgcca agactggtgc ctcgtttgtt ggacaatggt ggcaatattc
      721 tcatgatgca ggttgaaaat gagtatggtt cttacggaga agataaggct tacctgagag
      781 cgattcgaca gctaatggaa gagtgtggcg taacctgtcc cctctttaca tcagatggtc
      841 catggcgagc tactctgaaa gctggaacct taattgaaga ggacctcttt gtaacaggaa
      901 actttggttc taaggcacct tacaactttt cgcagatgca ggaattcttt gatgaacatg
      961 gtaagaaatg gccactcatg tgtatggagt tctgggatgg ttggttcaat cgctggaaag
     1021 aaccgattat cacacgggat cctaaggaat tggcagatgc agttcgagag gttttggaac
     1081 aaggctctat caatctttac atgttccacg gtggtacaaa ctttggtttc atgaatggtt
     1141 gctcagctcg aggaactttg gacctgccac aagttacgtc ttatgattac gatgcccttc
     1201 tggatgaaga aggaaatcca actgctaaat atcttgcagt caagaagatg atggcaacac
     1261 atttttcaga gtatccgcag ttggaaccac tctacaaaga gagtatggag ttggatgcta
     1321 ttccactagt tgaaaaagtt tctttgtttg aaaccttaga tagcttgtca agtcctgtag
     1381 aaagtctcta tcctcaaaag atggaggagc tgggacaaag ttatggctac ctactttatc
     1441 gaacagaaac aaactgggat gcagaagaag aaagacttcg tatcattgat ggtcgagata
     1501 gggcccagct gtatgtcgat ggtcagtggg ttaaaactca atatcagaca gagattgggg
     1561 aagatatttt ttatcaaggt aaaaagaaag ggctatctag gttagatatc ttgatagaaa
     1621 atatggggcg tgtcaactat gggcataagt tcttagcgga tacgcaacgt aagggaattc
     1681 ggacaggggt ctgtaaggat ctgcatttct tactaaactg gaaacactat ccactcccac
     1741 tagacaatcc tgagaaaatt gatttttcaa aaggatggac tcaaggacaa ccagcctttt
     1801 acgcttatga ctttacagtc gaagagccaa aagatactta cctagacttg tctgagtttg
     1861 gtaagggggt tgcctttgtc aatgggcaga atctaggacg tttttggaac gttggcccaa
     1921 ctctctcact ttatatccct catagctatc tcaaggaagg tgccaaccgc atcattatct
     1981 ttgaaacaga aggtcaatat aaagaagaga ttcatttaac tcgtaaacct acactaaaac
     2041 atataaaggg ggaaaactta tgacaattgt aggatgccgt attgatggac gtttgatcca
     2101 cggacaagta gccaatcttt gggctggaaa actaaatgtt tcacgcatta tggttgtaga
     2161 cgacgaagtt gtcaacaacg atattgaaaa gagtggtttg aaacttgcga caccaccagg
     2221 tgtgaaattg agtattttgc cagttgagaa agctgcagcc aatattcttg ctggcaaata
     2281 cgatagccaa cgtctcttta tcgtggctcg taaaccagac cgcttccttg gtttggtaga
     2341 agcaggtgta ccacttgaaa cccttaatgt tgggaatatg tctcaaacac cagaaactcg
     2401 ttctattaca cgttctatca acgtagtaga caaggatgtg gaagacttcc acaaactggc
     2461 agaaaaaggt gttaaactta ctgctcagat ggttccaaat gatccaattt cagacttttt
     2521 gagcttatta aaataggaaa aaatttttta ggaggtcatt gttatgatac aatggtggca
     2581 aattttactt ctcactttgt actcagctta tcaaatctgt gatgagttga cgatcgtttc
     2641 atctgcaggt tcccctgtat ttgctggttt cattactggt ttaatcatgg gagatgtgac
     2701 tactggttta cttatcggtg gtaacttgca actgttcgtt cttggggttg gtaccttcgg
     2761 tggtgcttct cgtatcgacg caacttctgg tgcggttctt gcgacagcct tctctgtttc
     2821 acaaggaatt gatgcaccgc ttgcaattac tacaatcgct gtaccagtag cagctctctt
     2881 gacttacttc gacgttcttg gtcgtatgac tactaccttc ttcgctcacc gtgtggatgc
     2941 tgcaatcgaa cgctttgact ataaaggtat tgaacgcaac tacttgcttg gtgcgattcc
     3001 gtgggctcta tctcgtgccc ttccagtctt ctttgccctt gcttttggtg gtgcctttgt
     3061 acaatcagta gtagacttcg ttgaagccta caaatgggtt gcagatggct tgacactcgc
     3121 aggacgtatg cttccaggtc ttggatttgc aatcttgctt cgttaccttc cagttaaacg
     3181 taaccttcac taccttgcta tgggatttgg tttgacagct atgttgactg ttctttactc
     3241 atatgtaaca ggtcttggtg gcgctgttgc tggtatcgta ggtactcttc ctgctgaagt
     3301 tgctgaaaaa attggtttcg tgaacaactt caaaggtttg tctatgattg gtatttctat
     3361 cgtaggtatt ttccttgcag tgcttcactt caaaaatagc caaaaagtag ctgtagcagc
     3421 accttctaca ccatcagaaa gtggggaaat cgaagatgac gaattctaat tacaaactta
     3481 caaaagaaga ttttaatcaa atcaacaaac gtagcttgtt tactttccaa ttaggttgga
     3541 actacgaacg tatgcaagct tctggttacc tttacatgat cttgcctcag ttgcgtaaaa
     3601 tgtatggtga tggaactcct gaattgaaag aaatgatgaa agttcatact caattcttca
     3661 atacttcacc attcttccat accattatcg ctggttttga ccttgccatg gaagaaaaag
     3721 atggtgtagg ttcaaaagac gctgttaacg gtatcaagac aggtttgatg ggaccattcg
     3781 ctcctcttgg ggatacaatc tttggttcac ttgtacctgc tatcatgggg tcagtcgcag
     3841 caactatggc tatcgctggc caaccttggg ggatcttcct ttggattgca gttgcagtag
     3901 cgtatgacat cttccgttgg aaacagttgg aatttgctta caaagaaggg gttaacctta
     3961 tcaacaacat gcaaagtacc ttgacagctt tgattgacgc tgcatctgta cttggtgtct
     4021 tcatgatggg tgctcttgta gcaacagtga ttaactttga aatttcttac aagttgccaa
     4081 tcggtgaaaa gatgattgat ttccaagaca tcttgaacca aatcttccca cgtttgcttc
     4141 cagcaatctt tactgccttt atcttctggt tgcttggtaa aaaaggtatg aactctacta
     4201 aagctatcgg tattattatc gtacttgctt tggctctttc tgcccttggt cactttgcac
     4261 ttggaatgta attccttatg actaaatcat taattttggt gagccatggt cgcttctgtg
     4321 aggagcttag aggtagcaca gaaatgatta tgggcccaca agacaacatt tacacagtag
     4381 ctcttcttcc agaagatggc ccagaagaat ttactgctaa atttgaagct gttattgaag
     4441 gattggatga tttcctagtc tttgcggatc ttctcggtgg gacaccttgt aatgtggtga
     4501 gtcgcttgat catggaaggt cgtgatattg acctttacgc agggatgaat cttccaatgg
     4561 tgattgaatt tatcaatgcg agccttacag gcgcagatgc ggactacaag agccgtgctg
     4621 cagaaagcat tgtgaaagtt aatgacctgt tagcgggctt cgatgatgac gaagatgaat
     4681 aatactcttc gaaaatctct tcaaactacg tcaacgtcgc cttgccgtag gtatatgtta
     4741 ctgacttcgt cagtcttatc cggcaacctc aaaacggtgt tttgagctga cttcgtcagt
     4801 cttatccggc aacctcaaag cagtgctttg agcagcctgc ggctagtttc ctacagattt
     4861 tagttggaac tcgattcaat tcatgtgaca acgtgaaaat cgttagagca ttttatatag
     4921 aatatacatg ggaatggagc ttactcccat tcccatattt aatagaaaaa gaggaactca
     4981 atgctacatt atacaaaaga agacttgctc gaattgggtg cagaaatcac tacgcgtgaa
     5041 atctaccaac agcctgatgt atggagagaa gcttttgaat tttatcaagc aaaacgtgaa
     5101 gaaattgcag cctttctaca agaaatcgct gataaacatg actatattaa ggttatcttg
     5161 acaggtgctg ggacttctgc ttatgtggga gataccttgc taccttactt taaggaagtc
     5221 tatgacgaac gcaaatggaa tttcaatgct attgcgacaa cagatatcgt tgccaaacca
     5281 gcaacctatt tgaaaaaaga tgtggcaact gtccttgtgt cttttgctcg tagtgggaac
     5341 tcgcctgaaa gtgtggcgac tgttgatttg gccaaatcct tggtggatga gctttatcaa
     5401 gtgacgatta cttgtgcagc agatggtaaa ttggctcttc aagctcacgg tgatgaccgt
     5461 aatctcttgc tcttgcaacc agctgtctct aatgatgctg gatttgccat gacttctagc
     5521 tttacgtcta tgatgttgac agctctcttg gtctttgatc ctacagaatt tgctgttaag
     5581 tctgaacgtt ttgaagttgt atctagtctt gcccgtaaag ttttagacaa ggtagaagat
     5641 gtcaaagagc tcgttgattt agactttaac cgtgtcatct atctaggcgc tggtcctttc
     5701 tttggacttg ctcatgaagc tcagctcaag attttggaat taactgctgg tcaagttgcg
     5761 accatgtatg aaagcccagt tggcttccgt cacggtccaa aatctcttat caacgacaat
     5821 acagttgttt tggtctttgg tacaacgaca gcctacactc gtaagtacga cttggacttg
     5881 gttcgtgaag ttgctggtga ccagattgct cgtcgtgttg tgcttttgag tgatcaagct
     5941 tttggtcttg aaaatgtcaa agaagtggtc cttggttgtg gcggtgtctt gaatgatatt
     6001 taccgtgtct tcccttacat cgtttatgcc caactctttg ctctattgac ttcactcaag
     6061 gtagaaaata aaccagatac accgtctcct acaggtacag taaaccgtgt agtacaaggt
     6121 gtcataattc acgaatatca aaagtaagac agtgtttatg aattcttgac aagaggattt
     6181 gtaaattatc agataaacca tagattgtca gtacgctttc tatggtttgt ttgcttgaga
     6241 gaaatagtaa aaggagaaca gaatgaaagc atacacagag cgtgtatttg gaaatgttga
     6301 gggcgaggat gtcttggcct atcgatttga gacagacggt ggctaccaac ttgaggttat
     6361 gacttatggc gcgaccatct tgcgctatgt cgcacctgac aaggctggaa attttgccaa
     6421 tgttatcttg ggatttgatg actttgatag ttatgtaggc aatagtccca agcatggagc
     6481 aagtgtaggt cctgtagcgg gtcgtattgc aggtgcgacc tttgagctca atggtaagac
     6541 ctatgacctt gaggttaata atgctagcaa ctgtaatcac agtggttcaa ctggttggga
     6601 ttccagcctg tttgaagttg aagaagtaag cgatcatggc ttgactctct acacagagcg
     6661 tacagatggg acaggagggt tccctggaaa tctcaagatt tggatcagtt atcacttgga
     6721 agaaactggt gcctatgaaa tcatctacaa ggtaacgacc gatcaggata cgctggtcaa
     6781 tccaaccaac cacagctatt tcaacttgtc tggtgatttc acgcagacga ttgaccgtca
     6841 tgtcttccaa ctaaacacag agggcattta ctcaatcgct cctgacggtg ttcctgccaa
     6901 aactccagaa gccaaccgtg atgtggtcaa acacatctac aatggtacct tgttgaagga
     6961 tatctttgca gaagaagatg agcaaatcca gctggcatca ggtttggatc acccatttgc
     7021 ctttcctgca ggccatgaca atgctggatt cctttatgac caaaattcag gtcgcttcct
     7081 gcttttcaag acagaagctc cttgctttgt ggtctacaca gcaaactttg tggatgaaag
     7141 tgtcatcata ggaggtcagc caatgctaca gcacaatggg attgctcttg aagcgcaagc
     7201 tttaccagat gccattcaca gtgaccttaa aggccaagtc attcttaaag ctggtcaaac
     7261 cttcaccagt aagacacgtt atgaacttgt tgtgaaataa aaaagctggt caaaaagtct
     7321 ttaaaattaa aataacgcat attgtcaggt gatgaaaagt ttgataatat gcgttttatt
     7381 atgaaatgat ttactttata ttatcctgaa atggtgttat ttttgagtta tctggcttga
     7441 ctttcttgat ggaaattata taatagttgt aacaataata attacaacga gaaaagagga
     7501 ccatttatga cctatgaata caagagtcac atttatttgg cagagacagt tttaaatgta
     7561 aaggatttgg ccagtcaaac agccttttat cagcaagtta tcggtttaga aatcttatct
     7621 caaactgaga cagagtccat tctaggcatt ggtggaaaag tcttggtaca gctgattcaa
     7681 gcacaagaga gcggagaagt gagggaacat tatggtcttt accatttggc cattctcttg
     7741 cccacacgca aggctttggc tgatgtcttg aaacacctga cagatttaca gattcctctt
     7801 gtcggcggtg cagaccatgg ttacagtgaa gctatttact tggaagattt ggagggaaat
     7861 ggcattgaac tctatcgaga taagccagtt tccacatggg atattcgaga agatggacgc
     7921 attatcggag tgacggaggc tcttgcggcg caggacattt atgagttggg ggaaagagta
     7981 gagcccttta tcctggcaga aggtacgaga atggggcata ttcatctttc cgtcaaggat
     8041 agtcgaaagt ccagccagtt ttatcaaaag gtgttagggc tagagaataa attcagtgtg
     8101 cctggcgcta gttggattgc ggctggagat taccatcatc atttagcagt taacgaatgg
     8161 agaggaaaag gtctggcttc gcgtaagcaa ggcttaccag gtttagccta ctatgtcatc
     8221 gaagtcgcac ataaaaaaga actgttaacg attgcccaac gagcacaaga agttgacgca
     8281 ccaatcaaat ggatgacatc gagcaaattg gaaatcacag actcagatgg catcgtgacc
     8341 cgtattcgtt tagcaaggta aaattaggaa tttttaataa gttataagta ttgctagttt
     8401 gctttttgaa cggtctttgg taacagaact gcgtgagctg gatgggtgag aattttgaca
     8461 aagaagctga ttagagatat aatgaagaaa aatcgagcaa ggaaaaagaa atgacaaatt
     8521 caaagtatat tactcgtttg aaacgttcag agggccagtt gcgtgggatt caaaagatga
     8581 ttgaaggaga tcgtgactgt gctgatattg tgacgcaact gacagcagtg aaatctagtg
     8641 tagagcgcgt gattgagatg ataattaccg aaaatcttac tgaatgtatc aatcagcccc
     8701 tagatgattc tgaagctcaa aaggaacgcc tagagaaggc tatctgatac ttgattaaac
     8761 ggaaataaat gaatgttttg agacgatatg tgcaaaggtc tattaactat tagaatagtt
     8821 taatgtaagt caaaagaagt tgtaaaaaat ctttaaaatt aaaaaacgca tagtatcagg
     8881 tattgaaatg tactgccccc caaaagttag attttttctg tctaactttt ggggggcagt
     8941 acataaaaac cttgatagta tgcgttttat tgtggggaga cttatactct tcgaaaatct
     9001 cttcaaacca cgtcagtttc accttgccgt agatatggtt actgacttcg tcagttctat
     9061 ccacaacctc aaaacagtgt tttgagctga cttcgtcagt tccatccaca accttaaaac
     9121 agtgttttga gcagcctgcg gctaacttcc tagtttgcac tttaattttc attgagtatt
     9181 acttcatctt ctcatgaaat tgagtttttg cccagctcct cttgattatt ctacttgacc
     9241 gggccaagta ttcataccac cttctacgtt ggtaacggtg aggccttggg agctgagaaa
     9301 ttggcaggta gatgcagagc ggactcctcc ttgacaaata acatagtatt catggtcagg
     9361 tttgagttct ttgtagcctt gctctaaggt acttaatgga agatttttgg cacctggtgc
     9421 atgtcctgct tggaattcat gtgcttcacg gacatcgata agttctagat tttcattttg
     9481 gtattttcca taaaagtcag ccatgctgat actagtttcc atattctact ccttctgggt
     9541 tagccatttt gtatagtgaa taagcgccgt caaggttttg gacggtaaat cctgcttgct
     9601 tgaggatagg ctctgcgata tagctgcgca aaccactgtg gcagctgacg atgtaggctt
     9661 gttttttgtc cagttcatca gcttcctcct tgacactttg tcagtttttg atacaatagt
     9721 acaaaattag aggaggtggg ctatgattca gaaacatgcg attcccattt tagagtttga
     9781 tgataatcct caggcggtta tcatgcccaa tcacgagggg ttggatttgc agttgccaaa
     9841 gaagtgtgtc tatgcttttt taggtgagga gattgaccgc tatgcgaggg aagtaggggc
     9901 gaactgtgtt ggcgaattcg tttctgctac caagacctat ccagtctatg tcatcaacta
     9961 caagggtgag gaggtctgtc tggctcaggc tcctgttggc tccgctccag cagcccagtt
    10021 tatggattgg ttgattggct atggtgtgga gcagattatc tctactggga cctgtggtgt
    10081 cctagctgat atagaggaaa atgcctttct agtccctgtt cgcgctttgc gagatgaggg
    10141 agccagttac cactatgtgg caccttgtcg ttatatggaa atgcagccag aggctattgc
    10201 tgctattgag gaagttttgg aagacagagg gattccttat gaagaagtca tgacctggac
    10261 gacagacggt ttttaccgag aaacggctga aaaggtggct tatcgtaagg aagaaggctg
    10321 tgctgttgtg gagatggagt gttctgctct tgcggcagta gctcaattgc gtggggttct
    10381 ctggggtgaa ttgttgttca cagcagattc tctagcggac ttggaccagt acgacagtcg
    10441 tgactggggc tcggaagctt ttaataaggc gctagaactg agtttagcaa gtgttcacca
    10501 cctttagttg tactggcaaa ggatttgttt tatcataaaa tgtctagctc atacttttca
    10561 aaaatatgtt taaacgaggt caccttcctc ttgtcctagg catgttgagg ttgggaaaaa
    10621 tctttaaaat cagaaaaacg tatcatatca ggtgatgaaa actttgatac tatgcgtttt
    10681 atgttggtaa gatttagagt gagatgaaat gatactcttc gaaaatctct tcaaaccagg
    10741 tcagcttcac cttgccgtag gtatatgtta ctgacttcgt cagtcttatc cggcaacctc
    10801 aaaacggtgt tttgagctga cttcgtcagt tctatccaca acctcaaaac agtgttttga
    10861 gcaacctgtg actagctttc taagcgatgc cttggttctc attgcctata atcaaaaaga
    10921 gaaattttct cctgaaaagc atatagagta gctggcgtta aaagctcctg tcttgctttt
    10981 ttgacctata gtcacatcta tcaagtattg ttcttgccta agctatcaat aaaaaggtgg
    11041 cattttttag gcttggtgtt agtagatttt gccttatcct atctaagtca tttcgagctt
    11101 tttatggtac aatggaaaca tgttattcaa attatctaag gaaaaaatag agctaggctt
    11161 atctcgttta tcgccagccc gtcgtatttt tttgagtttt gccttggtca ttttactagg
    11221 ctctcttctt ttgagcttgc cctttgtcca agttgaaagc tcacgagcga cttattttga
    11281 tcatcttttc actgctgtct ctgcagtctg tgtgacaggt ctctcaaccc ttccagtagc
    11341 tcacacctat aatatatggg gccaaataat ctgtttgctc ttgattcaga tcggtggtct
    11401 agggctcatg acctttattg gggttttcta tatccagagc aagcaaaagc ttagtcttcg
    11461 tagccgtgca actattcagg atagttttag ttatggagaa actcgatctt tgagaaagtt
    11521 tgtctattct atttttctca cgaccttttt ggttgagagc ttgggagcta ttttgcttag
    11581 ttttcgcctt attcctcaac ttggctgggg acgtggtctt tttagttcca tttttctagc
    11641 gatctcagcc ttctgtaatg ccggttttga taatttaggg agcaccagtt tatttgcttt
    11701 tcagaccgat ttactggtca atctggtgat tgcaggcttg attattacag gcggccttgg
    11761 ttttatggtc tggtttgatt tggctggtca tgtaggaaga aagaaaaaag gacgtctgca
    11821 ctttcatacg aagcttgtat tattattgac tataggtttg ttgttatttg gaacggcaac
    11881 tactctcttt cttgagtgga acaatgctgg aacgattggc aatctccctg ttgccgataa
    11941 ggttttagtt agcttttttc aaacagtgac gatgcgaaca gctggctttt ctacgataga
    12001 ttatactcag gctcatcctg tgactctttt gatttatatc ttacagatgt ttctaggtgg
    12061 ggcacctgga ggaacagctg ggggactcaa gattacgaca ttttttgtcc tcttggtctt
    12121 tgcacgaagt gagcttctag gcttgcctca tgccaatgtt gcgagacgaa cgatcgcgcc
    12181 gcgaacggtt caaaaatcct ttagtgtctt tattatcttt ttgatgagct tcttgatagg
    12241 attgattctg ctagggataa cagccaaagg caatcctccc tttatccacc tcgtatttga
    12301 aaccatttca gctcttagta cagttggtgt aacggcaaat ctgactcctg accttgggaa
    12361 attggctctc agtgttatca tgccacttat gtttatggga cgaattggtc ccttgacctt
    12421 gtttgttagc ttggcagatt accatccaga aaagaaagat atgattcact atatgaaagc
    12481 agatattagt attggttaag aaaggaaaga gcatgtcaga tcgtacgatt ggaattttgg
    12541 gcttgggaat ttttgggagc agtgtcctag ctgccctagc caagcaggat atgaatatta
    12601 tcgctattga tgaccacgca gagcgcatca atcagtttga gccagttttg gcgcgtggag
    12661 tgattggtga catcacagat gaagaattat tgagatcagc agggattgat acctgcgata
    12721 ccgttgtagt cgcgacaggt gaaaatctgg agtcgagtgt gcttgcggtt atgcactgta
    12781 agagtttggg ggtaccgact gttattgcta aggtcaaaag tcagaccgct aagaaagtgc
    12841 tagaaaagat tggagctgac tcggttatct cgccagagta tgaaatgggg cagtctctag
    12901 cacagaccat tcttttccat aatagtgttg atgtctttca gttggataaa aatgtgtcta
    12961 tcgtggagat gaaaattcct cagtcttggg caggtcaaag tctgagtaaa ttagacctcc
    13021 gtggcaaata caatctgaat attttgggtt tccgagagca ggaaaattcc ccattggatg
    13081 ttgaatttgg accagatgac ctcttgaaag cagataccta tattttggca gtcatcaaca
    13141 accagtattt ggatacccta gtagcattga attcgtaaag agggatgacc cctctttttt
    13201 gatgcccaag atggcaaata gagacagaag ccccttgtct tctagtaaaa gttcttcaaa
    13261 ggctggactt tatggtaaaa tagaaagaag tgacaagaga gagtaatact caatgaaaat
    13321 caaagatcaa actaggaaac tagctacggg ctgctcaaaa cagtgttttg aggttgcaga
    13381 tagaactgac gaagtcagta acatctatac ggcaaggcga cgttgacgcg gtttgaagag
    13441 attttcgaag agtataagaa aaaatcagcc ccctaaagga gcagattatg aagttattgt
    13501 ctatcgccat ttctagctat aatgcagcag cctatcttca ttactgtgtg gagtcgctag
    13561 tgattggtgg tgagcaagtt gggattttga ttatcaatga cgggtctcag gatcagactc
    13621 aggaaatcgc tgagtgttta gctagcaagt atcctaatat cgttagagcc atctatcagg
    13681 aaaataaagg ccatggcggt gtggtcaatc gcggcttggc agaggcttct gggcgctatt
    13741 ttaaagtagt tgacagtgat gactggtgga tcctcgtgcc tacttgaaaa ttcttgaaac
    13801 cttgcaggaa cttgagagca agggtcaaga gatggatgtc tttgtgacca attttgtcta
    13861 tgaaaaggaa ggtcagtctt gtaagaagag tatgagttac gattcagtct tgcctgttcg
    13921 gcagattttt ggctgggacc aggtcggaaa tttctccaaa ggccagtata tcatgatgca
    13981 ctcgctgatt tatcggacag atttgttgcg tgctagccag ttctaattgc ctgaacatac
    14041 tttttatgtc gataatctct ttgtctttac gccccttcag caggtcaaga ccaggtacta
    14101 tctgcctgtc gatttctatc gttatttgat taggcgtgag gaccagtctg tcaatgagca
    14161 agtgatgatt aagtgcattg accagcaact caaggtcaat cgactcttgg tagaccagct
    14221 tgatttgtcc caagtgagtc atcctaaaat gcgagaatat ctgctgaatc atattgaaat
    14281 cacgacggtg atttccagta ccctgctcaa ccgatctgaa acagcggagc atctggcaaa
    14341 aaaacgccaa ttgtggacct atattcagca ggaaaatcca aaagtctttc aggctattcg
    14401 taagaccatg ttgagccgtt tgaccaaaca ttctgtcttg ccagatcgca aactgtccaa
    14461 tgtcgtctat caaatcacca aatctgttta tggatttaa
//
LOCUS       DAVLSL010000038        14280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLSL010000038 DAVLSL010000000
VERSION     DAVLSL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.039"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..825)
                     /locus_tag="VXN30_000932"
     CDS             complement(<1..825)
                     /locus_tag="VXN30_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1357580.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEK"
     gene            1152..1544
                     /locus_tag="VXN30_000933"
     CDS             1152..1544
                     /locus_tag="VXN30_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357581.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            1566..2357
                     /locus_tag="VXN30_000934"
     CDS             1566..2357
                     /locus_tag="VXN30_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357582.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            2350..2991
                     /locus_tag="VXN30_000935"
     CDS             2350..2991
                     /locus_tag="VXN30_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357583.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            2995..3627
                     /locus_tag="VXN30_000936"
     CDS             2995..3627
                     /locus_tag="VXN30_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1357584.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            3655..3942
                     /locus_tag="VXN30_000937"
     CDS             3655..3942
                     /locus_tag="VXN30_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357585.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            4088..6601
                     /gene="secA"
                     /locus_tag="VXN30_000938"
     CDS             4088..6601
                     /gene="secA"
                     /locus_tag="VXN30_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1357586.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            6682..7713
                     /locus_tag="VXN30_000939"
     CDS             6682..7713
                     /locus_tag="VXN30_000939"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1357587.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            7715..8746
                     /locus_tag="VXN30_000940"
     CDS             7715..8746
                     /locus_tag="VXN30_000940"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1357588.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            8785..9153
                     /locus_tag="VXN30_000941"
     CDS             8785..9153
                     /locus_tag="VXN30_000941"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1357589.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            9143..10246
                     /locus_tag="VXN30_000942"
     CDS             9143..10246
                     /locus_tag="VXN30_000942"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1357590.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFIEASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            10265..12280
                     /gene="recG"
                     /locus_tag="VXN30_000943"
     CDS             10265..12280
                     /gene="recG"
                     /locus_tag="VXN30_000943"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1357591.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            12596..13576
                     /locus_tag="VXN30_000944"
     CDS             12596..13576
                     /locus_tag="VXN30_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1357592.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(13930..14073)
                     /locus_tag="VXN30_000945"
     CDS             complement(13930..14073)
                     /locus_tag="VXN30_000945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357593.1"
                     /translation="MADRTDEVSSKHRFEVADRTDEVSSKHRFEVADRTDEVSNIYTA
                     RRS"
BASE COUNT         4197 a         2611 c         3164 g         4308 t
ORIGIN      
        1 tttttcgagt attttaacta atgcctcttc tttggttagt cgtaataaag ttattccttt
       61 atcaatcaaa tcggagttat gttgtagtat tcccaagcgt acttgagaga atccaagaat
      121 atcgtatatt tttaattctt ctgctaatac caaaagttgt agggtgattg tttcacattc
      181 tttgaataaa tgatgttgaa aatatacagt ggagagattg gctaaaaaag atgataaaaa
      241 tgcttttgtt tctcggaagt atttatattt ctcgatggtt ttcataattc tatcaataag
      301 aaggggaaga ttttcagaag gaaagaaaaa gagaatggtt ccaagcaatt tcaaatcact
      361 aatgtaccat gtatccatag gttcaagata gtcccaaatt tcttcataca aatctttcaa
      421 gacgtggttg tttttgaatc ctttagttcg taactctgtg acaatttttg tatgacgata
      481 gatattttga ataggaacat cgtgatgtgt cttaagatat ttttgacatt tctcagtgag
      541 ttcaaccatt tttctagtat cttgaaaagt agacggattt tggctctcta caataatatc
      601 tcgcctttta ctaggatggt attcgttgca tatatacttg aattctgcca agctcatatc
      661 aatttgttca agaagaaata ccatgttttc aaaactggga ataactttac cgtgttcaat
      721 atttgttaga gttgttcgat gtatgacttg tccacataca tcttgttggg tcaatccttt
      781 tgactttcga atttctttaa aaatttgtcc ataatcccat ctcatattgt tcctcctagg
      841 taaatgtgaa tattttctac atttataaaa aacgtagaaa aaggtggtat tattatatca
      901 aaaaggaggg aaaaagtcat gggctttaaa aaatatttga agaatttacc gaaaaactct
      961 ggatttttga tttggagttg gattcaactt atctggtttg aaacatggtt ttggggataa
     1021 agaaattaga gtctatacat aataagtaaa ggaggaattt gtatgatata ccatcgttta
     1081 gaataagtct atatttaaaa tagaagttat aaggaatctt cttgaataaa aaatgaaagg
     1141 aaacagaaaa aatgaaaatt aagaaattat tgaaaatggt tattcctgtt ttaatgataa
     1201 gtgctgttgg aactactttc gtagaagcaa atcagatagg tgcttttagt aattttgtta
     1261 ttactaccag ttataagaga acaggttatt tgactaaaga aaatgaaggt gcggaatata
     1321 tcatgaactt aaatccttgt agaaatttac atcccatgac tgttaagcat cgtatagtaa
     1381 attctaatgg agaagctcgt agtggagaat cgttaacaac ctgtggtact cgttcaactc
     1441 atggtaattg ggcaacggtt ggctatgttt atgcagcaga tatggcaaga caaaattggt
     1501 gggatttatc agctgccatt tcaggtagtt ggtcaccaaa ttaattcgta aagtaggtat
     1561 aatttatgaa gattcctctc ttaacttttg caaggcataa atttgtttat gtcttgctta
     1621 ctttgctttt tcttgctttg gtttatcgtg atgttttgat gacttatttc ttttttgata
     1681 ttcatgcgcc cgatctagct aaattcgatg gacaagcaat taaaaatgac ttattaaaat
     1741 cagcattaga ttttcgtatt ctccagttca atctaggttt ttatcaatca tttattattc
     1801 caatcatcat tgttttgcta ggttttcaat atattgagct gaaaaataaa gttttacgat
     1861 tgagtattgg aagagaagtg agttatcaag ggttaaaaag aaagttgact ttgcaagttg
     1921 caagtatccc ttgtttgata tatttagtga ctgtgctgat aattgcaatt ataacctatt
     1981 tctttgggac tttttctcct cttggatgga attctctatt ttctgatgga agtggtttac
     2041 aaagactcct agatggagag ataaaaagct atttgttctt tacttgtgtc ctactaatcg
     2101 gtattttcat caatgcaatc tattttttac aaatagttga ttatgtgggg aatgtgactc
     2161 gttcggcaat cacctatttg atgtttcttt ggcttggttc tatgctgctc tatagtgcct
     2221 tgccttacta tatggttcct atgacgagtt tgatgcaagc tagctatggg gatgtaagtt
     2281 tgatgaaact ctttactcct tatatccttt atattgtccc ttatatggtg cttgaaaaat
     2341 atgaagataa tgtttaagaa ttttaacaat atttggctaa agagaaagat cgttttacta
     2401 cttcgtatag ttctgatgat gattttgata aactatctat tgtcaacagc ggttcaaaag
     2461 caggatgctg ttatcttttt caagagagaa ttgatttcaa tcttttccta taatgactat
     2521 tctgaagcga atttagaaat ccccaaactc ttgttaaacc tttcgctttt catggtagga
     2581 tggctctctg tcattttact tgaaagtgat ttggcagacc attaccatca cttgattcgc
     2641 tatcaatcaa gctccttttt cgattataca aggaaacgat tggttgtcat ttctaaattt
     2701 tttactcaag atttacttgt ctggttcctt ggtttacttc cgctaggaat tcatttcaaa
     2761 acagtcgcac ttttcttttt acttgctcag ttaatgattt tgtacttact actgtcttat
     2821 ctgatagcac tgattagtgc gggcgctggt ttttcctttt ttctctattt tttagcattt
     2881 gtgggacaag aatggatgat ggatcatatt gtaacagtgt atttactact cttaagctta
     2941 ttagttatgt tgattgttag tcgcttggaa gagaaattta agaaaggata aacgatgaga
     3001 cttgaaatta taaatggaca gaaaatttat gggaaaagac ctattttaaa tcagttgaat
     3061 ttggtgtttc aatcaggaaa aatttatgga cttaaaggtg ataatggatc tggcaagacg
     3121 gttcttttaa agatacttgc tggttatatt aagcttgaca aaggaaaagt tcttcaagac
     3181 gggaaagtct acgggataaa aaatcattat attcaggatg caggaatttt aattgaaaaa
     3241 gtcgagtttt tatctcattt atccctgaga gaaaatttgg aactgttaag gtatttttca
     3301 tctaaagtta cggaaaaaag aattgcctat tggattcaat actatgattt acaggaattt
     3361 gaagacattg aataccgtca tttatcctta ggaacaaagc aaaaaatggc cttgattcaa
     3421 gcctttattt cctctccttc tatactcttt ctcgatgaac ctatgaatgc tttggatgag
     3481 aagagtgtga ggttaaccaa acaggtcatt ttatcttacc tgaaaaaaga aaatggtctg
     3541 gttatcctga catcgcacat atcggaagat atttcagacc tttgtacaga tgtattagtt
     3601 gtcgaaaatg gacatataca aatgtaaagg atatacaatc ctaggagatg gcttatggca
     3661 catctaaaat catttattac acgctattcc aaggtttata ttggtttagt tctgctgatc
     3721 tggctgtctt tcttctttat cccttgggat agagcgattc tgggggtaag gattgacatc
     3781 ttcatcatac agaaaatctt gctagctttt ggaattctgt ccattctcat ggccttgctg
     3841 tccaagaaag tcagtctctt tgtttttgga ctgatttgct gtctttctct ttggattaac
     3901 ttatttatca catttgccat tttgccgatt tttggcaatt aaacagtcat aaaagtcgga
     3961 gaggttagct tgaaaactaa cctctttttc cttttcaaaa tggggattct tccttgaaaa
     4021 taatcagtaa ttgtgctaaa attaaaggaa cattctaaaa tattcggaat ttaaagtaag
     4081 gaaaaacatg gctaatattt taaaaacaat tatcgaaaat gataaaggag aaatccgtcg
     4141 tctggaaaag atggctgaca aggttttcaa atacgaagac caaatggctg ctttgactga
     4201 cgaccaacta aaagcaaaaa cagttgaatt taaggaacgt tatcaaaatg gagaatcact
     4261 ggattcattg ctttacgaag catttgcggt tgtccgtgaa ggtgccaaac gtgtcctagg
     4321 tctcttccct tataaggttc aggtcatggg ggggattgtt cttcaccatg gtgacgtgcc
     4381 agagatgcgt acaggggaag ggaaaacctt gactgcgacc atgccggtat acctcaatgc
     4441 cctttcaggt aaaggggttc acgtagttac ggttaatgaa tacctgtcag aacgtgacgc
     4501 gactgagatg ggtgaattgt actcttggct tggtttgtca gtagggatta acttggctac
     4561 caaatctcca atggagaaaa aagaagccta tgagtgtgat attacttact caactaactc
     4621 agaaatcgga tttgactacc ttcgtgacaa catggtcgtt cgcgccgaaa acatggtaca
     4681 acgtccgctt aactatgcct tggtcgatga ggttgactct atcttgattg acgaggctcg
     4741 tacacctttg attgtatcag gtgccaatgc ggttgaaacc agtcagttgt atcacatggc
     4801 agaccactat gtaaaatctt tgaacaaaga tgactacatc atcgatgtgc agtctaagac
     4861 tattggtttg tctgattcag ggattgacag ggctgaaagc tacttcaaac ttgaaaacct
     4921 ctatgacatc gaaaacgtgg ctttgactca ctttatcgat aacgcccttc gtgccaacta
     4981 catcatgctt ctcgatattg actatgtggt gagcgaagag caagaaatct tgattgtcga
     5041 ccaatttaca ggtcgtacca tggaaggtcg tcgttattct gatggattgc accaagctat
     5101 tgaagccaaa gaaggtgtgc caatccagga tgaaaccaag acatctgcct caatcacgta
     5161 ccaaaacctc ttccgtatgt acaagaaatt gtctggtatg acgggtacag gtaagactga
     5221 ggaagaagaa ttccgtgaaa tctacaacat tcgtgttatt ccaatcccaa caaaccgtcc
     5281 tgttcaacgt attgaccact cagaccttct ttatgcaagt atcgaatcta agtttaaagc
     5341 ggttgtcgaa gacgttaagg ctcgttacca aaagggtcaa cctgtcttgg ttggtacagt
     5401 agcggttgaa actagtgact acatttctaa gaaattggtt gcagctggtg ttcctcacga
     5461 agtcttgaat gccaaaaacc actatagaga agcccaaatc atcatgaatg ctggtcaacg
     5521 tggtgccgtt accatcgcaa ccaacatggc gggtcgtggt accgacatca agcttggtga
     5581 aggtgttcgt gaacttggag gactttgtgt tattggtaca gaacgtcatg aaagtcgtcg
     5641 tatcgataac cagcttcgtg gacgttcagg tcgtcaagga gatccaggtg agtcacaatt
     5701 ctacctatct cttgaagatg atttgatgaa acgttttggt tctgaacgct tgaagggaat
     5761 ctttgaacgc ttgaacatgt ctgaagaggc cattgagtct cgcatgttga cgcgtcaggt
     5821 tgaagcagct cagaaacgtg tcgaaggaaa taactacgat acccgtaaac aagtccttca
     5881 atacgatgat gtcatgcgtg aacaacgtga gattatctat gctcaacgtt acgatgtcat
     5941 cactgcagat cgtgacttgg cacctgaaat tcagtctatg atcaaacgca cgattgaacg
     6001 tgtcgttgat ggtcatgcgc gtgccaaaca agatgaaaaa ctagaggcaa ttttgaactt
     6061 tgctaagtac aacttgcttc ctgaagattc tattacgatg gaagacttgt caggcttgtc
     6121 tgataaggcc atcaaggaag agcttttcca acgttccttg aaggtttacg atagtcaggt
     6181 ttcaaaacta cgcgatgaag aagcagttaa agaattccaa aaagttttga ttctacgagt
     6241 ggtggataac aagtggacag atcatatcga tgcccttgat caattgcgta acgcggttgg
     6301 acttcgtggc tatgctcaga acaaccctgt tgttgagtat caggcagaag gtttccgtat
     6361 gtttaatgat atgattggtt cgattgagtt tgatgtgaca cgcttgatga tgaaagcaca
     6421 aattcatgaa caagaaagac cacaggcaga acgtcatatc agtacaacag cgactcgcaa
     6481 tatcgctgct caccaagcaa gtatgccaga agatttggat ttgagccaga ttggacgcaa
     6541 tgaactttgc ccatgtggtt ctggtaagaa atttaaaaac tgtcacggta aaagacaata
     6601 aaatgagata gtttagaggc ggatatcttg tgaaaagtaa atttttactg ggtatccgtt
     6661 tgctttataa ggagatgagt tatggtattt acagcaaaaa gctctaaaat aaatatagaa
     6721 gaagttcgtg ccttgtcaaa attagaaggt caggctttgg agaggaaatc acagagagat
     6781 caagagctag aagccattat acgtggagaa gaccagcgaa ttctcttggt aatcgggcca
     6841 tgctcatctg ataacgaaga agctgtcctt gaatacgcta agcgtttggc agtcctacaa
     6901 gaagaagtgg cagatcgtat ctttatggtt atgcgtgttt atactgccaa accccgtacc
     6961 aacggagatg gctataaagg cttgattcac cagcctaacg cgacagaagc gcctagtctt
     7021 atcaatggaa tcaaagccgt tcgccatctt cactatcgtg tcatcacaga aacagggatg
     7081 acaactgctg atgaaatgct ttatcctgaa aaccttccgc ttgtagatga tttgatttct
     7141 tacatggcag ttggtgcccg ttcagttgaa gaccagcaac accgctttgt ggcaagtggg
     7201 gcaggatttt ctactggttt taaaaatcca acctctggaa atctcaatgt catgtttaat
     7261 gggatttatg ctgctcaaaa caaacaaagt ttccttttct taggaaaaga agtagaaaca
     7321 actgggaacc cgctttcaca cgctattctt cgtggtgctc ttaatgagta tggaaaaaat
     7381 attcccaact actattatga caatttaatt gataccattg cccagtatga gaaaatgggc
     7441 ttggaaaatc cttttatcat cattgatacc aatcatgaca attctggtaa gcagtatatt
     7501 gaacagatcc gaattgtccg ccagaccttg attaaccgtg cttggaatga aaaaattaag
     7561 cagttcgttc gtggttttat gattgagtct tatctagaag atggtcgaca aaatgagcca
     7621 gaagtatttg gtaagtctat cacagaccct tgcctgggtt gggataacac agaagctctt
     7681 gtcagagaaa tttacaaaac gttaggagaa taagatggca tttattgaaa aaggtcaaga
     7741 aatcgatatg gaagtcatca aggctgaaac ccaattgtct gcggaagcct tgagactcaa
     7801 ggaaagccgt gacagggaat tggcagatat tatttcaggg gaagatgacc gtattctctt
     7861 ggtgattggt ccttgctctt ctgataatga agaggcggtc ttggaatatg ctcgccgttt
     7921 atccgccttg caaaagaagg tagcggacaa gattttcatg gtcatgcgcg tgtatactgc
     7981 taagcctcgt accaatggag acggctataa aggattggtt caccagccag atacttctaa
     8041 ggctccaagc ctgattaacg gcttgcaggc tgtgcgccag ttgcactacc gcgtgattac
     8101 agagactggt ttgacaacgg cagatgagat gctttatccg tcaaatctga tcttggtgga
     8161 tgacttggtc agctaccatg ccgttggagc tcgttctgtg gaagaccaag agcaccgctt
     8221 tgtggcttct gggattgatg caccagtagg gatgaaaaat ccaacctcag gaaatttggg
     8281 tgttatgttt aacgccatct atgctgctca aaacaagcaa accttccttt atcatgggca
     8341 ggaagttgag acatcaggta atcctttggc ccatgttatc ctccgtggag cagtcaacga
     8401 gtatggcaat tatatgccga attactacta tgaaaaccta ctccaagcca ttgaacgcta
     8461 tgaaaccatg ggacttgaaa atccttttat cctcattgac accaaccatg ataactcagg
     8521 caagcaatat atggagcaga ttcgaattgt tcgccagacc ttgcagaatc gtgattggaa
     8581 tgagaaaatt aaaaagacgg ttcgaggatt tatgattgaa tcttacctag cagatggtcg
     8641 tcaaaaccaa ccagagatct ttggttgctc tattactgac ccttgcctag gttgggaaaa
     8701 tacagaggcc ttggtagaag agatttatgt taccttgaca aaataagtga aaaggatgga
     8761 gttggggaat ctcaactcct tttgatgaga atgatagttg gacacggaat tgacatcgaa
     8821 gaattggctt cgatagaaag cgcagttaca cgacatgaag gatttgctaa gcgtgtactg
     8881 accgctcagg aaatggagcg cttcaccagt ctcaaaggac gcaggcaaat agaatattta
     8941 gctggtcgct ggtcggctaa ggaggccttt tccaaggcta tgggaacggg cattagcaag
     9001 ctcggttttc aggatttgga agtcttgaac aatgaacgtg gggcgcctta ttttagtcag
     9061 gcaccatttt caggaaagat ttggctgtct atcagccaca ccgatcagtt tgtgacagcc
     9121 agtgtcattt tggaggaaaa tcatgaaagc tagtccacat agaccaacca aggctctgat
     9181 tcatctggga gctattcgac aaaatattca gcaaatgggg gctcatatcc ctcaaggaac
     9241 gctcaagttg gctgtggtca aggccaatgc ttatggtcat ggagctgttg ccgttgccaa
     9301 ggcaattcaa gatgatgttg atggcttttg cgtttccaat atcgatgaag ccattgaact
     9361 cagacaagct ggactcagca agccaatcct cattttagga gtttctgaaa tcgaagctgt
     9421 tgctctagct aaagaatatg acttcacctt gacagtggct ggactggagt ggattcaagc
     9481 actcttagat aaggaagtgg acctaactgg attgacagtc cacctcaaga ttgattcagg
     9541 gatgggacgg attggtttta tagaggcaag tgaggttgag caggctcaag atttgctcca
     9601 acaacacggt gttcgtgttg aaggaatctt tacccacttt gctactgctg atgaggaatc
     9661 agatgactat tttaatgccc agttagaacg gtttaaaact attttagcta gtatgaagga
     9721 agttccagag ctggttcatg ctagcaattc tgcaacgact ctttggcatg tagagactat
     9781 tttcaatgcg gttcgtatgg gagatgccat gtatggtctc aatccaagtg gagcggtctt
     9841 ggatttgcct tatgatttga taccggcctt gaccttggag tctgctctgg ttcatgtcaa
     9901 gacagttcca gctggagctt gcatgggcta tggagcaact tatcaagcgg atagcgagca
     9961 agtcatcgcg accgtgccaa tcgggtatgc agatggatgg acaagagaca tgcaaaattt
    10021 ctctgtcttg gtagatggcc aagcttgccc aattgttggc agggtttcga tggaccaaat
    10081 cactattcga ttgcctaagc tttatccgct aggaaccaag gtaaccttga ttggctccaa
    10141 tggggataag gaaatcactg caactcaggt agcgacctac cgcgtaacca ttaactatga
    10201 ggtggtttgc ctcctcagcg accgtattcc gagagaatat tattagaaaa gaaaggagtg
    10261 gagcatgaat ctacatcaac ccttgcatgt cttgcctggt gtgggaccaa agtcagcaga
    10321 aaaatacgcc aaactaggaa ttgaaaactt gcaagatctc ttgctctact ttcctttccg
    10381 ttatgaagac ttcaaaacca agcaggtgct ggagctggaa gacggtgaga aggcagttct
    10441 ttctggtcag gtagtgactc ctgctagtgt ccagtattat ggtttcaagc gcaatcgcct
    10501 gcgttttagt ctcaagcagg gagaggtcgt ttttgcggtg aatttcttta accagcccta
    10561 tctggctgat aaaatagagt tgggagcaac ccttgctgtc tttggaaaat gggaccgcgc
    10621 taaggctagt ctgactggga tgaaggttct ggctcaggta gaagatgacc tccagcctgt
    10681 ttatcgtctg gctcagggaa tcagtcaggc cagtctggtt aaggtcatca agacggcttt
    10741 tgatcaggga ctggacctct tgatagaaga aaatctgccc cagtctttac tagacaaata
    10801 caaactcatg tcccgttgtc aggcagtccg agctatgcat tttccaaagg atttggcaga
    10861 atacaagcag gctcttcgcc gtataaagtt tgaggaactc ttttatttcc aaatgcagct
    10921 gcagacgctc aagtctgaaa atagaattca gggaagtggt ctggttctgg attggtctca
    10981 ggaaaaagtg acagcagtta aagcaagtct tccttttgcc ctgacccaag ctcaggaaaa
    11041 gagtttgcag gaaattttaa ctgatatgaa gtccgaccac cacatgaatc gtctcctaca
    11101 aggggatgtg gggagtggaa aaacggtagt cgctggcttg gccatgtttg cggcagtgac
    11161 agcaggttat caggctgccc taatggtacc aacagaaatc ctcgcagagc aacactttga
    11221 gagtttacag aacctttttc ccaatttgaa actggctctc ttgacaggtt ccttgaaagc
    11281 tgcagaaaag agagaagtct tggagaccat tgccaagggt gaggctgatt tgattatagg
    11341 aactcacgct ctgatacaag atggggtgga gtatgctcgt cttggtttga ttattatcga
    11401 tgagcagcac cgttttggtg tagggcaaag gcgtatttta cgggaaaaag gtgacaatcc
    11461 agatgtcctc atgatgacgg cgactcccat tccacggacg cttgccatca cagcctttgg
    11521 agatatggat gtttccatta tcgaccagat gccagcaggt cggaagccta ttgtgacgcg
    11581 ctggatcaaa catgagcaac tacctcaggt cttgacttgg ttagaggggg aaattcaaaa
    11641 aggttcccaa gcctatgtca tctctccttt gattgaagaa tcagaagctc tagatttgaa
    11701 aaatgccatt gccttatcag aagagctgac ggctcatttt gcaggcaagg cagaggtggc
    11761 tcttctacat ggtaggatga agagtgacga aaaagaccag atcatgcagg atttcaagga
    11821 gagaaagacg gatattctgg tttcgacgac ggttattgag gttggggtca acgttcccaa
    11881 tgcgactgtc atgattatca tggatgccga tcgcttcggg ctcagccagc ttcaccagct
    11941 tagaggtcgt gtcggtcggg gggacaagca gtcctacgct gttctcgttg ccaatcccaa
    12001 gacggattct gggaaagacc gcatgcgcat catgacagaa acgaccaatg gatttgtcct
    12061 tgcggaggaa gatttgaaaa tgcgtggttc tggtgagatt tttggaacca gacagtcagg
    12121 acttccagag ttccaagtgg ctgatattat cgaagatttt ccgattttag aagaagcaag
    12181 aaaggttgct agctacatta gttctataga agcttggcaa gaagatccag agtggcgcat
    12241 gattgccctt catctggaaa agaaagaaca tctggattaa gctttctcta aggaaaactt
    12301 aagctagttt ttaggtttgg ctcttatact aaagtcatca aaaagaaacg aggactctca
    12361 tatgacagta acgattaaag taaattacca aaccactttc caaaagaagg aagcaaaaaa
    12421 ctagtataaa cagaagagag agcgaaatgc tctttttcgt ttctaaaact actttcagcc
    12481 catcatccta aaagtaaaga atctaaattc actttctatt tatccttctt tcttgcattg
    12541 attacataga tatgctacag ttgtggtaac gattacaaaa taaaaggagc atgctatgaa
    12601 aaatccagct ttgctagaag aaattaagac ctatagagga agggatgagg ttccggaaga
    12661 ctttgatgct ttctgggatg gggaagtaaa aaatgtttcc acgcttccat cctaccactt
    12721 ggaggaaaga gatttccaca ttcctcaagt caagtgctat gagttaacat ttgaaggaag
    12781 caaggaagga aaggtctatg cacgcattgt tcttccaaag agtgaggaga aggttccact
    12841 aatattccat tttcatggtt atatgggacg tggctgggac tgggccgaca tgctgggctt
    12901 caccgtagct ggttacggtg ttgtttccat ggatgtgcgg ggccagtcag gttactcaca
    12961 agacggcttg cgttctcctt taggaaatac cgtgaagggg catattatcc gtggtgctat
    13021 ggaaggtcgg gaccacctct tttataagga tgtttatctg gatatttacc agttggtcga
    13081 aattgttgct agtctgtctc aggttgatga gaagcgtctt tctagctatg gtgcctcaca
    13141 aggaggggct ctagctctag ttgcagcagc gctcaatcct cgaattcaga aaacagttgc
    13201 catttatccc ttcttgtcag acttcagacg ggtgattgag attggtaata ctagcgaggc
    13261 ttacgacgaa cttttccgtt atttcaagtt tcacgacccc ttccatgaaa cagaggagga
    13321 aatcatggcg acccttgcct atatcgatgt caaaaatctt gcccatcgta tccaaggtga
    13381 ggttaagatg attacgggct tggacgacga tgtttgctat cccattaccc agtttgcgat
    13441 ttataatcgt ctgacctgcg ataaaaccta tcgcatcatg cctgagtatg ctcacgaagc
    13501 catgaatgta tttgtcaatg accaagtcta caactggctc tgtggaagtg agattccttt
    13561 taaatatcta aaataaggag tcgactctaa gcacaaaatc ttaaaaatta caaacacgca
    13621 tagtatcagg ggattaaaaa aacttgatac tatgcgtttt atcatggaaa tatagtaaaa
    13681 tgaaacaaga ataggacaaa acgatcagga cagtcaaatc gatttctaac aatgttttag
    13741 aagcaaatgt gtactattct agtttcaatc tattatattt atagaatttt ttgttgctag
    13801 atttgtcaaa ttgcttaaaa taattttttt cagaaagcaa aagccgatac ctatcgagta
    13861 gggtagttct tgctatcgtc aggcttgtct gtaggtgtta atacttttca aaaatctctt
    13921 taaaccacgt cagcttcgcc ttgccgtata tatgttactg acttcgtcag ttctatctgc
    13981 cacctcaaaa cggtgttttg agctgacttc gtcagttcta tctgccacct caaaacggtg
    14041 ttttgagctg acttcgtcag ttctatctgc cacctcaaaa cggtgttttg agctgacttt
    14101 gtcagtctta tctacaacct caaaacagtg ttttgagcat catgcggcta gcttcttagt
    14161 ttgctctttg attttcattg agtataaaaa cagatgagtt tctgttttct ttttatggac
    14221 tataaatgtt cagctgaaac tactttcaag gacattatta tataaaagaa ttttttgaaa
//
LOCUS       DAVLSL010000039        13381 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLSL010000039 DAVLSL010000000
VERSION     DAVLSL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.040"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(407..2134)
                     /gene="ezrA"
                     /locus_tag="VXN30_000946"
     CDS             complement(407..2134)
                     /gene="ezrA"
                     /locus_tag="VXN30_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1357594.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2216..4162)
                     /gene="gyrB"
                     /locus_tag="VXN30_000947"
     CDS             complement(2216..4162)
                     /gene="gyrB"
                     /locus_tag="VXN30_000947"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1357595.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4177..4740)
                     /locus_tag="VXN30_000948"
     CDS             complement(4177..4740)
                     /locus_tag="VXN30_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1357596.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4825..5379)
                     /locus_tag="VXN30_000949"
     CDS             complement(4825..5379)
                     /locus_tag="VXN30_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1357597.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5390..6613)
                     /locus_tag="VXN30_000950"
     CDS             complement(5390..6613)
                     /locus_tag="VXN30_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1357598.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKGG
                     KVPFKRKKVVLKQIK"
     gene            complement(6650..9100)
                     /locus_tag="VXN30_000951"
     CDS             complement(6650..9100)
                     /locus_tag="VXN30_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1357599.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9300..10229)
                     /locus_tag="VXN30_000952"
     CDS             complement(9300..10229)
                     /locus_tag="VXN30_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1357600.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10369..10656
                     /locus_tag="VXN30_000953"
     CDS             10369..10656
                     /locus_tag="VXN30_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1357601.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10690..12024)
                     /gene="ciaH"
                     /locus_tag="VXN30_000954"
     CDS             complement(10690..12024)
                     /gene="ciaH"
                     /locus_tag="VXN30_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1357602.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12014..12688)
                     /gene="ciaR"
                     /locus_tag="VXN30_000955"
     CDS             complement(12014..12688)
                     /gene="ciaR"
                     /locus_tag="VXN30_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1357603.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            12906..>13381
                     /locus_tag="VXN30_000956"
     CDS             12906..>13381
                     /locus_tag="VXN30_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1357604.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDE"
BASE COUNT         4087 a         2854 c         2371 g         4069 t
ORIGIN      
        1 gtggactaaa gttgtacaag aaaagtgcaa ataagaaatc tccagattag gaactatccg
       61 tgagttctct agtctggaga tttttcaata tacttcgtta ttggacggtt accaagttac
      121 tcaaagtaca gatttgaagc tgcagataag actgacgaaa tcactttaat actaaatgaa
      181 aatcaaaaag caaactaaga agctagcctc aggctgtact tgagtacggt aaggcgacgc
      241 tgacgtgatt tgaagagatt ttcgaagagt ataaaacact gttttgaggt ggtggataaa
      301 tcggctgggt aaaaactcaa tctcaggaga aaatgaagta aatctcccca caactataga
      361 aaaagatttg attctgctcc tcacacaata aaatcttttt cttttattaa aaacgaatcg
      421 tttcacgtgt tttctcatat gaggtaacaa agcgattggt tacaccaggc tctgccactt
      481 ccaatgcttg agaaatcttg tcaaatgaag cgtgataatc aaattctttt tcaaaaatat
      541 ctaaagcttc gttaaatgct tcttgaatgc gttcatcaaa tgagcgatag cggttagaat
      601 attgcaagag ttgctctgtc aaagttgcat attgtacaat attataagtt tccgtttcta
      661 aagcttccat atcattcgtt gcaatttcaa gaactcgggt aacagattca atgttaatca
      721 ttttttgttc taactcaacc attaaatcct cggtattatt gcttgccgta aagaataact
      781 tcaagaaagt ttgtggaata cctggcagat tgcgtttttc catgtatcgc ttgatagtat
      841 ggagacgatt gacataaaca ttggcctttt gacgtgcatt aatatcatct ttctcaattt
      901 gtgtcaggcg ctcactaact gaaatttgct catcttcaat atcttttagt tgagtttgta
      961 aatcctcaag attttcttca agaactgaat aagcttgggt tggttcttct tgatttgaag
     1021 ttacctcaac aatagctgcc tcaaaactct ctaattctgt ctgaatacga cgaacatggc
     1081 ttgcagctgt ctcaggaagt aaataggtct tgttcaaacg tgcaatatct tctcccaata
     1141 aagtattatt ctctttcata tgttgaaggt aagttggaag agttgcaagt agattttcca
     1201 ctactttctg agcagcaatt tctcgagtaa aaatatcata caaggcattg atttcctctt
     1261 gtgcctgtcc attctcatat tcggcattat ccaattccaa ctgacgaata ttctcttggt
     1321 ttttcttgaa tgcttcataa agcaagtgga aacgcgcttc aatatccgtt tcaacaaaat
     1381 gataattagc atcaattagt ttacgataac cggattccaa atcctgtaat tgatctggca
     1441 attctgtaga aagcgtcgta accaaggctg gaacacgatc cacaatatga cttaaggcca
     1501 aaatgtgatt ttctgtatta tccaaaatca ctgcggcttc cacagggtca cccgatgaat
     1561 tcaaggttac aaattgtgaa aattcagatt ggatattttc taattgtttt tcaatttcat
     1621 ccaaggcttg accatactgt tctgaatttt cagcaactct atgctgaagt tcctcaaata
     1681 aatccaaagc atgaagaaca cgaccactat ttttagattc ttgcttctct aagtctgcca
     1741 aagcattgcg aattgccgca atatcttcgt caatcaaagt aatttgactc tcaatttggt
     1801 caatttgatg actggccttg agaaaacgaa atgaatggtt atagccttct gcttcaaaga
     1861 gattattttc aatatcggca aaagagttga gagataaatc gacccatttt tgattccatt
     1921 cacggaaagc cacttgactt tgtccaatca agtgcatatt ttttacagct tctacttcat
     1981 catttactgg aagattgtat agttcttctt ttctttcttc tagcgcctct aatctcccct
     2041 cgtttcgctt acgtagaaag attgccacta cataagccag aactaaaatg actgcaattg
     2101 caaccattaa ataaattagt tgtccattag acatatcaaa ctcctttttt acttgaaaca
     2161 atcgtaacga ttatatcata ttttttagac caagggaact acttctccct attttttaga
     2221 catcaagtgt actatagaca gcattttctt cgataaactc acgacgaggc tctactcgat
     2281 cccccatcaa catatcaaag attttatctg cttctgcagc atcatctaca gaaactctag
     2341 ccatcaagcg atgttcggga tccatggttg tttcccacag ctgatgatcg tccatttcac
     2401 ctagcccctt ataacgctga atagtcggtt tggtacgacc ttcactataa cgggctaaag
     2461 cttcttggag tttgatttct tgatctgcac ccggctggat atattcttta atctcgcttc
     2521 caaccttgac accatagatt ggtggttggg caatataaac ataaccagct tctaggattg
     2581 gtttcatata acgataaatc aaggttaaaa gaagggtacg aatgtgggct ccatcgacat
     2641 cggcatcggt catcaaaacg agtttttggt aacgggcttt cgaaacatca aattctgcgc
     2701 caaatcctgt tcccatggct gtgaaaagac tacgaatttc ttcgttagct agaatcttat
     2761 ccatacttgc tttttcaacg ttcaaaatct taccgcgaat tggaaggata gcctgaaact
     2821 cacggttacg accagatttg gctgatccac cagctgagtc tccttcgacg atgaagagtt
     2881 ctgtttcagc agggttatta gaagaacagt ctgctagttt ccctggaagg ttggaaattt
     2941 ccaaaccaga ttttttacgt gtgacttcac gcgcacgctt ggcagccaca cgagccttgg
     3001 cagctaaaat ccctttttcc acgatacgtt tggcaatctg tggattttcc atgaggaaat
     3061 cggagaaggc ttcactgaag aggcgattgg taatcttgac cacttcgcta tttcccaatt
     3121 tggtcttggt ttgtccttca aactgtggat ttgggtgttt aactgagata actgcagtta
     3181 atccttcgcg aacatcttcc cctgttaaat tatcttcatt gtctttcagt aacttattct
     3241 tacgagcata atcattgata acacgagtca gtgccgtacg gaaaccttgt tcatgcgttc
     3301 caccttcatg ggtatgaata ttattggcga aactcatgac attttcatgg taacctgttg
     3361 tgtactgcat ggctacctca actgtgatat catccatctc accgtctgta tagattggtg
     3421 tatcaaagat tacatccttg ttctcgttga tatattcaac gtaactagca atcccacctt
     3481 cataatgata atgcttggtt tgttccaaac cttggcgctt atctgtaatt gaaatttgaa
     3541 gaccgcgatt tagaaaggcc aactcttgaa tccgtttatt taatttatca aaatcaaaga
     3601 ttgttgtttc agtgaaaatt tctgggtccg gtgtgaagtg aaccgttgtt ccagttctat
     3661 ctgtatcccc aaccacctca aggtctgcaa caacatgacc acgacggtat tcttggtaat
     3721 gaatcttacc gtttttatgg acatgaacgt ctaattgagt ggaaagggca ttaactactg
     3781 acgaccccac cccgtgaaga ccacctgaaa ccttgtatcc accaccgccg aactttcctc
     3841 cagcatgaag gactgtaaag acggtctcaa cagcaggacg gcctgttttt tcctgaatat
     3901 cgactgggat accacgccca tcatccacaa cagtaatcga atcatctggc tcaataaaaa
     3961 cttgaatatg gctggcaaat cctgccaagg cctcgtcaat tgagttatca acaatttccc
     4021 agactagatg gtgaagacct tcttttgagg ttgatccaat gtacatccct ggacgcatac
     4081 gaacagcctc taagccctct aaaacttgaa tttgactggc atcataatcc tgtgcctgca
     4141 gatttttgat ttcttctgtc atcttattcc tttttcttat atgtctaata cttgtcttaa
     4201 attcacgata ctggtaaaca agtgggtatc aagtcctgca gcttgacctg cttcgatatc
     4261 aatcggccga tcaccaatga caagaccaga gctaatctga tacttttctc ttaaataaag
     4321 catggattcg ggatttggct ttctcttaaa gcctgagcta gaagtcacca cttctgtaaa
     4381 ataagctgct atagaggttt tttctaaaat ttccaaaacc tgatcatttc gatgagagac
     4441 caaaaaatga cggccacctt gatttaaaat gtcttccaat aggtcagaaa ctccttcaaa
     4501 taaaatcggg tgttcaagct ctctggcttc attttccttg tacttttcta aaaaattctc
     4561 taaattggga gcgaatgtct caatcgcaaa aggagtagaa acctttaaag cttgatagac
     4621 actgtcatgg tcttgtgtga taccatacag tgccaatgtt tcaacaaatg cagctgttga
     4681 agtttcataa ttatccagta aagttccacc taaatcccag atgtaatcgt gatatttcat
     4741 acccttcatt atagcatttt tttatgaaaa aagcgatgat ttccctgagt tttccacata
     4801 aaaaacgaga gaataactcc cgttttactg atttttacca aagacatctc gatagagcat
     4861 tcctgttcgt aaactctctg cgtcccctcc tagttgctcc accaactcgt aggcaaaggc
     4921 aagggctgtt gaaggacccc gactggttgt caactgacca tctaccacta ctgtttcctt
     4981 gacgtagtga ccatcaagga tttgctcttg aacgccgtca taacaagtgt atcgcttatt
     5041 tttcaatatc tctgcttgat tgagggcaat tggtgccgca caaatggctg ctagtttctt
     5101 cccttcttgc tcgaagcttt gcaattcttg aatcaaggtc tgattatcac gtaaatgtgc
     5161 agaaccaggc atacctccag gaagaacaat catatcatag tctgataaat ctccatcaaa
     5221 gacatgatct gctcttactt ggattgcatg cgaacccgtt acttgctctt caaaaccaac
     5281 catatcacat gtgatattgg ctcgacgcaa gacatctaca actgtcaagg cttcaatttc
     5341 ttcaaagccc tgagctaaca taactgctac ttttaccatg attttctcct tatttaattt
     5401 gttttaatac aacctttttc cgtttgaatg ggacttttcc gcccttttct tcagctaaat
     5461 tagtctggta actcatcgat aaaagcaaac caacaccaat caaattactg ataatagctg
     5521 atcccccttg cgaaatgaaa ggcaagggaa tccccgtcaa aggaagtagt ccagtcacag
     5581 caccgatatt ctcaaagatg tggaagagca acatcataat caaacctgtg gaaatataag
     5641 tgtagaactg gttatttgat ttaagagtaa tcttcaacat acggtaaatc aacatgagat
     5701 agagggcaat aaccaggaca gagccaataa agccaaaatc ttctgcaata accgtaaaaa
     5761 tcatatctga ctctcgaact gggataagca gattcgaagc attaaatccc tgaccaaata
     5821 agccaccact cccaatggca atctgccctt gagcctgctg gtaagtcgtt gtttgggcaa
     5881 actcaaaggg attgagccaa gccaaaatcc gattaatttg gtaggtcggc attccaatct
     5941 ggtgaagaaa agctcgtccg tccttgctaa taaagatagc taagaaacca gcaactcctg
     6001 ttacagcagt cacaaatact gggataataa ttttccaaga aacccctgat aataaaacga
     6061 ttcctgagaa aatggctaca aaaaccaaag ccgtccccaa gtcactttga agtgctaaaa
     6121 gaactaggac tggaatggta aagagaatca tccagaaaat taacaaaaag tccagcggaa
     6181 ccgtgcgtct ccattcctta tgtttctttg taaattggac aatgacacga gccaacatga
     6241 ggatatagga tatcttcata aattctgacg gttgaaatag ggtaattcca tttattgata
     6301 cccagttttt ggcacccgtt gatgcaacta agcttggatt ataaaataca atcggcaaga
     6361 tcataagtcc caagcctaaa atatatagaa agggggtcac cttccaaaga aattctgtat
     6421 taaagagcat gaccacaaaa ccaatcacaa gccccaaggc aatccaggcg acctgctgcc
     6481 ctaaaatggg cagaatatta ttgggataat catgactaac ggctatatag atagccacca
     6541 caccgatgac cagtagaaaa aatactggca agagcaaact ataatcgact ctagagtcga
     6601 gagaacgttt catatgtact aaccttatac tttcatacaa tactatttat caaagttcat
     6661 taaaaaatct atcaatagcc tcgtcaactt cggatcgaga gatggtttta acagtcgctt
     6721 cttctgctag agatgctact atttgtttgc cgtatcgttt tccgacgatt ctcctatcca
     6781 aaataagagt taaggaacgt tggtattcac gtctcatact tcttcccaaa gcctgtttta
     6841 aacgaataat ggccattggc aattgataat cataaaaggc atttttccct tcttgattca
     6901 gttcttgatt aatctttttc gtcaagggtt cttgaggatt ttggaaagga agcctcggta
     6961 caacttgaat cacagaagga tggcttgaaa aatcaactcc ctcccagaaa cttgctgcac
     7021 caagcaagat ttgttgttca cctttttcaa agcgtttctt tagctgatga acatccccat
     7081 ttttatactg ggccaagtgg ctaactgtaa gtaaatccga tactgctaga agcatgtctt
     7141 tagcggtaaa gagaaccaaa atcggttgct ggaaagcttg aatttccact agtaaagcag
     7201 ctacctctct ggcatagact tctaaggagg tttctgttac cagagggaaa tctttgacca
     7261 taaccacttc ttgttcctgt tttccccgag attcaatctt gacaaatttg gcttcaggat
     7321 agcctaaaag gtctgccaaa gaaaccctct gactaatctc aagagtagcc gatactccca
     7381 agacttggca actctcagga agtaaagagg acagaagagt acggcctgat tttgtagaag
     7441 aaatctgaat tttcttttga ctcgtttcag ttacttcaag ccagtaatca ccttcagctg
     7501 taaaatagcg aaccagctca tcaaagcctt ccacttctaa ttctgaaaaa tactgatgga
     7561 gattgtccag agaatctaag atatttttcc tagatttacc agactgaaat tgttctatca
     7621 agtagagaca ctcaaagcga atactttcta gtatccgttg ttgaaccctg ttttcttctc
     7681 ctactaaagc cttatcaatt aaatcgataa tagattgtat atcgtaggtc tcttgaagca
     7741 gattttctag agctaacaaa atcttttgga cttcatcaat aatcagtaaa cggtcactga
     7801 caaattcagg attatcttca agtctggtca cgagataggc atgattagtc actaaaagct
     7861 tgcaagtctc tgccctttct tgactacgtt tccaaaaatc ttccgtcaca aataagctct
     7921 gggatgataa attcccatca tgacgaaggt ctgctagaaa atgttggtaa cggtagagtt
     7981 gcccgatttc atccaaatct cctgtctctg tctcagtaag ccagaccaag acttgcattt
     8041 taaagcgtct aaataagcga ttttcatcat tttcctgcaa ggaatgataa aaggcatcca
     8101 acttcagata attttgtggt ccctttaagc tatgaatatc tgtatggaac acttccttga
     8161 gacgtttacc ttcttcttcc atgatttgat tttgaagaat ctttgtcgga acactaagaa
     8221 caatttgtcg ctctttggat tgagataaag cgggtaagag atagccatag gttttcccaa
     8281 tccctgtcgg cgcttgaatc agagagaaag gttcatcttt caatagcaag ccaacctctt
     8341 tagcaaaact ttcttgttcc tccctcactt caaggttcaa cagagaaata tttttagaaa
     8401 agtcttgaga tagttttcgt agctccagag aagctgccgt tttcttaaaa tacagacctt
     8461 gaacttggac caagtctgga gaactcagga tagattggtt gcgataagtt tcctcaataa
     8521 ccaggtaaga ctcatatagg agagcgtcag ccatttccag caagcgttcc aagagacctt
     8581 taggaagctg ggtcatcttt tcccgtaaaa aaagaagtaa ttctgctgta gcttgggcat
     8641 ctgaaagggc tgtgtgtgcg tgtttaagag gaattcctaa ttctcgacac aaaatcggca
     8701 agctatattt ttccagttca gggaaaaaga cctgggccaa ttcgaccgta tcaacacgag
     8761 ggtttcttag ctcatagcct tcaaaaaata aattttccgc caagagatta gcatcaaact
     8821 gaacattatg ggctacaaaa atcccatcct ccaccaagtc aaagattttt ctggcaactt
     8881 gcgaaaaatc aggtgcttgc gccagacgtt ggtctgtcaa tcctgtcagt tctttgatat
     8941 gagcatccaa gggttcatgt ggattgacat ccgtcgtata gtgatcgacg atttctccat
     9001 cctcaatcac gacaattccc acttggataa ttttagcctt actacctgtg ctagttgcct
     9061 ctaaatccac cacaacatag cgattaccaa tcgttttcat tataaaaaat tataccaaaa
     9121 aaagggccat ttggcacctg caaacatagg ataattttca aactcaagaa cactactttt
     9181 cttagaaatc ccatcgaata gagcattcta gcctgctgat ttttttaaaa tagtgaagaa
     9241 atacgaaaaa ttgagagaag aaatgttttc atcttccttc tctcaacgaa tcatgtattt
     9301 tatttaacca tgtcaggatc gtagtcataa aatcctgtat caaggaaacg gtttttaggg
     9361 tgaagtttac gaacttcttc atccagcaag aaggcttggt catggctaaa gttgccctct
     9421 tgaatcaagc tacgcgcttg gataaagagt tttgcaatct caacaaagtt ctgaccagga
     9481 gtgtagagcc cttctagttt ccagcgagta aagccatgct ccaccaattc tgttaatttg
     9541 atcatcaaat caaggtcatt gttggcaaag atatgggtcc catgattatc ttcaaaaatg
     9601 gaatagtggc tctctggatc acttggctca gccaagaaga ggtcacgttt atgcgtcttt
     9661 tcatcatcga tatgtgtaaa gttatagtag ttttgcaaga gtggacgttt agaatgatgg
     9721 atgacgctag caccgtaaac caaaacttca gcaggaattt ccaaaatctc tggcattttg
     9781 aaaagttcag ctgatggaat ttcacgcgcc aaaacagcct cagatgcgcc agccttttgt
     9841 ccccagaagt taatctgacg actgctagtt accatggttg aagcatcgta gatggtctta
     9901 aatgaataac catcgcggtt aactacgtaa aagacgcctg catccccaat cgtaatatag
     9961 tctgtcttga tttcttccaa gaagtctaag aaaggcttga tacggtccat catatcttgg
    10021 tgcatgagag cattgaccgc aacgatcaat tccttaccag catcatgaac caacttagcg
    10081 atttcacgta attggtcata actaaaggtc gttggcagac gaaggccaaa atctttctca
    10141 ccgacataga tacggtctac gccagcttcg agtagttgtt caacttgttc aatactttca
    10201 gcagttgctg taatgacaat cttttccata agaaaaatta taccacattt ctccaaaatc
    10261 agccatgctt tgaaaatgaa aagggacttt attctttttg taacctttct gtaataattc
    10321 tctgccgaaa aaatgataaa ataggtatgt actgttaagg agagaatcat gcccgtaaga
    10381 aaattacaat cctatgaggt agactatcaa gaagaattaa accagcagct tcctcattac
    10441 caagcttata cacctgaagc gcaatctgat gccaatctca aggaaatcct attttttatt
    10501 aatatcgctg ttttttgtat ctgtattgct atctttagtt ttatcttttt agcattaaaa
    10561 ttatcaactg ctcttgcctt tgccgcagca atcggattca gcttacttgt tttaaaagtt
    10621 caacggtcta ttatcaaacg aaaacgtaga agataaatcc aaaatatcgc cccaattgga
    10681 gcgatatttt tattttttct ttttagatgg tgtctgaatg gcaatcttca cttcaaagat
    10741 tgttcccttg ggtttattat ctttgacagt aacagttcct tttagagcat ctacaatttg
    10801 cttggctagg gataatccta aaccaaaacc acctttttgc cgggttctag ctttgtctac
    10861 tcgataaaaa cggtcaaaaa ttttcttttt atcttctgtc gaaataccga ttccattatc
    10921 agaaacaagt aaataaagat tgcgatcggt cgccgagata agaaaatcaa tttcaccatc
    10981 ctcctcagta tacttgacgg cattatcgaa aagaatggtc atcagttgtt tcagaagaag
    11041 ctgatctgtg acaattgttc gatggatacg attttcaaaa cggaagacac gattattttc
    11101 cgaagcaatc atctcatagt ttgtgaaagt tgtattaaaa aagctagttg gaacttctgc
    11161 aagctccggc ttaatcccat catctctccg agctaagttc agcaagcttg tcgttaaaaa
    11221 acgcatattt cggacttctt ccaaactcga tgcaatgctt tcgctcacat ccataatggt
    11281 agcttctggc ttacgaaaaa gggtctctaa gcgattttgc aaaactgcga gtggagttcg
    11341 taactcatga ctggcatttt ccacaaaaga ctgttgcttc tgcatactct caagcagggg
    11401 cctaacactg accctagcta gatagagact ggcaagtaaa gacaaaatcc agaaactagc
    11461 catcacgacc acaatcaatt gctcatgctt ttgactagcc tgctccaact gactggtatt
    11521 aatcaagaca gcagcatact tgatattggt tgaaaccgaa ctaatattgg tctccatcaa
    11581 aatcacacga tagatttctt cctgcccata gctattaaaa acctgaatct ggtagatatg
    11641 tcctagttct ttcttttcta acttaatctt atccaagccc aaaaatcgat ttccagaaag
    11701 aagttgagta aagtctttat caaagagaat gacttccgta ttggaactga cattaggttt
    11761 tatttctact ttactagcgt ccgccctagc attttctaaa tctttaatct cttctgttgc
    11821 cctatttata gccagctgaa taactgcttg aggattttca ctcagtccat gaagcttatc
    11881 gtccaccgaa gtataaagac tcgaatgcat gacttgtaaa ataatcagag tcattgtaga
    11941 aaaaatcagg gtgaagacac cgaagttgcg gataaaataa ctaaagtcat ccgcatacca
    12001 tgttttttta agtttactga acatctttta aaagataccc aacactacgc aaggtttgca
    12061 aattctctgc aaaagtggtt ccctttaatt tcttacggac ttttgaaaca tagacttcga
    12121 caaccgaaat cgttgtatca ctatcaaatc cccatagacg gtcaaaaatc tgcgtcttag
    12181 gcaaaatcac attttgattt tgaaggaaat aaactagtaa atcgaactct ttccccagca
    12241 attcgacagg agtatcttca actttaacgg tattggttga taaattaacc acgatattcc
    12301 cataagtcaa ggtgttttca ttaaacttcc ctgaacgttt gagaagggcc tgaattcgca
    12361 ttttaagttc ttctaggtag aaaggtttgg tcagataatc atccgctccc agttcaaatc
    12421 catgtccctt gtcatccaaa ctttccttgg cagtcataat cagaactggt gtcgtaattc
    12481 ccttttcacg caattctttt aagacttgga aaccattttt ttctggcaac atcaaatcca
    12541 gcaaaatcaa gtcatagaca ccactctcag cttcgtagag accttcttct ccatcaaata
    12601 cctgcataac atccgcaaaa tcgtctaaaa agtcaaatac tgaatttgac agacctaggt
    12661 catcctcaac caataagatt tttatcatga gaaactcctc cttattaaaa ctattatacc
    12721 aaatttgcct taaaaaaaac tcaactctct gcattttaca tgagatagct gagttttctt
    12781 tggctctata atatttgtag tgggtaaatc ccctatggat attatggagc ctattttatt
    12841 gtagaaaaaa agtcccatat gacctataat gaaaagcgac aaaacaactc attagaaaga
    12901 ttcatatgga acaattacat tttatcacaa aactgctcga tattaaagac ccaaacatca
    12961 agattctaga tatcatcaat atggataccc acaaagaaat tatcgctaag ctggattatg
    13021 aggctccatc ttgccctgat tgtggaagtc taatgaagaa atatgacttt caaaaaccgt
    13081 ctaagatccc ttacctcgaa acaactggta tgccttctag aattctcctt agaaaacgcc
    13141 gtttcaagtg ctatcactgt tcaaaaatga tggttgctga gactcccctg gtaaagaaaa
    13201 atcaccaaat ccctcgtatc atcaaccaaa aaattgctca aaagttaatt gaaaaaattt
    13261 ctatgactga tattgcccat cagctggcca tttcaacttc aactgtcatt cgcaagctca
    13321 atgactttca ctttgagcat gatttttctc ggcttccaaa gattatgtct tgggatgagt
    13381 a
//
LOCUS       DAVLSL010000040        13250 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLSL010000040 DAVLSL010000000
VERSION     DAVLSL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.041"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            74..154
                     /locus_tag="VXN30_000957"
                     /pseudo
     CDS             74..154
                     /locus_tag="VXN30_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(313..2136)
                     /gene="lepA"
                     /locus_tag="VXN30_000958"
     CDS             complement(313..2136)
                     /gene="lepA"
                     /locus_tag="VXN30_000958"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1357605.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPESVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(2215..2943)
                     /locus_tag="VXN30_000959"
     CDS             complement(2215..2943)
                     /locus_tag="VXN30_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1357606.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            complement(2945..4612)
                     /gene="recN"
                     /locus_tag="VXN30_000960"
     CDS             complement(2945..4612)
                     /gene="recN"
                     /locus_tag="VXN30_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1357607.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKERIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVHFSKGKFSRGGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            complement(4619..5050)
                     /locus_tag="VXN30_000961"
     CDS             complement(4619..5050)
                     /locus_tag="VXN30_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1357608.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            complement(5043..5858)
                     /locus_tag="VXN30_000962"
     CDS             complement(5043..5858)
                     /locus_tag="VXN30_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1357609.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQIFAEIKEAVERAHSQFKNE"
     gene            complement(5851..6726)
                     /locus_tag="VXN30_000963"
     CDS             complement(5851..6726)
                     /locus_tag="VXN30_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1357610.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLTYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            complement(6723..6935)
                     /locus_tag="VXN30_000964"
     CDS             complement(6723..6935)
                     /locus_tag="VXN30_000964"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1357611.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            complement(6913..8253)
                     /locus_tag="VXN30_000965"
     CDS             complement(6913..8253)
                     /locus_tag="VXN30_000965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1357612.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEKGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            complement(8381..9019)
                     /gene="udk"
                     /locus_tag="VXN30_000966"
     CDS             complement(8381..9019)
                     /gene="udk"
                     /locus_tag="VXN30_000966"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1357613.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            complement(9127..9429)
                     /locus_tag="VXN30_000967"
     CDS             complement(9127..9429)
                     /locus_tag="VXN30_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1357614.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            complement(9670..10548)
                     /gene="truB"
                     /locus_tag="VXN30_000968"
     CDS             complement(9670..10548)
                     /gene="truB"
                     /locus_tag="VXN30_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1357615.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVVFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            complement(10558..11832)
                     /locus_tag="VXN30_000969"
     CDS             complement(10558..11832)
                     /locus_tag="VXN30_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1357616.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            complement(11928..13187)
                     /locus_tag="VXN30_000970"
                     /pseudo
     CDS             complement(11928..13187)
                     /locus_tag="VXN30_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
BASE COUNT         3713 a         3173 c         2423 g         3941 t
ORIGIN      
        1 ttttgttgtg tttatagttt gttatataaa aataaacaca caaacaaata ctccaagcat
       61 ttttctgttc taatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggtta
      121 ctcaaaacac agttttgagg ttgtagatga aactgacgaa gtcagtaacc atacatacgg
      181 taaggcgacg ctgacgtggt ttgaagagat tttcgaagag tataaaaact aaaaaagcag
      241 accatctaag cctgctttac tattgattct tatataaatt tcctgtgaac aaggaaaggc
      301 atttctgata acttattctt catccatact caagacgctg aggaaggctt cttgcggaac
      361 ttcaactgat ccgatggatt tcatgcgttt cttaccagct ttttgttttt caaggagttt
      421 acgcttacga gaaacgtcac caccataaca tttagcaagt acgttcttac gaagggcctt
      481 gatatcagta cgagcgacaa tcttgtgtcc aatagccgct tggattggaa cctcaaattg
      541 ttggcgaggg atgattttct tgagcttatc aacgatgagt ttcccacgtt cataagcaaa
      601 gtccttatga acgataaagc tgagggcatc caccttgtct ccgttgagaa ggatatccat
      661 tttcactagc ttagatgggc gatattctga caattcgtag tcaaaacttg cataaccacg
      721 tgtcgaagac ttaagtttat caaagaagtc aaagacgatt tcagcaagag gaatttgata
      781 gataacattg acacggttat catcaatata gtccatagtc acaaagtccc cacgcttacg
      841 ctgagctagc tccattactg ctccgacgaa ctcctgtggt accatgattt gcgccttgac
      901 ataaggctct tcaatggtcg caatcttagt tgggtctgga aactcagatg ggttagacac
      961 atccatagac tcaccgtcgg tcaaattaac tttgtaaata acagacggag ctgtcatgat
     1021 gagatcaata ttgaactcac gctctaaacg ttcctggata acatccatat ggagaagtcc
     1081 aagaaatcca caacggaaac caaatccaag tgcctgagat gtttctggtt caaactgaag
     1141 actagcatca ttcagttgca atttttcaag cgcttcacgc aggtcattgt acttgtttga
     1201 ttcgattggg tagagacccg caaagaccat aggattcatc tgcttataac catgtaatgg
     1261 ttctgccgca ggattggttg ccaaggtaac ggtatcaccc acacgagtat cctgaaccgt
     1321 cttgatagac gccgcaatgt aaccaacatc accagtcgca aggaaatcac gaccaaccgc
     1381 ttttggtgta aaaataccga cttcggccac atcaaaggtc ttactattgc tcatgagctg
     1441 aatcttatca ccaggtttga ccactccgtc catgacacgc acttggagga taaccccacg
     1501 gtaagcatcg taaacagagt cgaaaatcaa ggccttaagt ggcgccgtca catcacccgt
     1561 tggtgctggt actttttcta caatttgctc gaggatttct tcaatcccaa taccagcctt
     1621 ggcagaagcc aaaactgctt cactggcatc caaaccaatc acatcttcaa tctctgtacg
     1681 cacgctctcc ggatctgcag ccggcaggtc aattttatta atgataggca tgatttccaa
     1741 atcattatcc aaagccagat aaacgttggc aagagtttga gcctcaattc cttgagccgc
     1801 atcgaccacc aaaatagcac cctcacaggc agctagcgaa cgtgaaactt cataggtaaa
     1861 gtcaacgtgc cctggtgtgt caatcaagtg gaaaatataa gtttccccat cttttgcagt
     1921 gtaattcaac tcgatggcat tcaacttaat agtaattcca cgttcccgct ctagatccat
     1981 gctatccaaa agctgggcct gcatttcacg acttgaaacc gtctctgttt tttccaaaat
     2041 gcggtctgct agagttgatt tcccgtggtc aatatgggcg ataatagaga agttacggat
     2101 cttcccctgt cgttttttca attcttctaa gttcatgatt ctcttccttt cagggtatct
     2161 atttattata aattgttttt gatattttga caagaccata ccctgctagg agtactaatc
     2221 ttcagcgaca aagccgtcat tttcgataaa gtggtgttct gtcattcctt ggtctgtaaa
     2281 gacaatcccg tgaaggacac caccataaac agctcctcca tccattccaa tcttgccatc
     2341 ttctgtagtc caaagctcag atgtaccgcg ttcttgctgt aacaaaccat agaccggtgt
     2401 atgaccgaag acaatggttt ttccagtatg attttcagct ccgtggaatg gttttctaag
     2461 ccatactttt ttataatctg ttgtttcatg ccagtcgtcc aaagtcaaat caatacctgc
     2521 gtgaacaaat atatacttgt ctgtctctac tacaaatggc atttgacgaa tgaattcgac
     2581 caagtctgac gcttcagcgg caacccgctt ggcatcttct actccatcaa ctggtgcatc
     2641 caagggacga cctaggatag agttaatggt tgtatctcca ccattgcgac gataatggtc
     2701 ataactttct tctgggtcat ctagccaagt caaaaacata tactcgtggt ttccagacaa
     2761 acagatagcc ccttgattgt ccaccaagtc cttgaccatt tcaagaacac ggtgactatc
     2821 ctcacctctg tcaatcaaat cacctagaaa gagcaactgg gtctgaccat cccaggtttt
     2881 aagaaggtct tccagcatcc cagcttttcc gtgaacatct ccaattacat aataatctgt
     2941 catcttattt ctccctgttt ctcaacaatt ctctggcttg cgtcagggct gcttctgtca
     3001 catcatcacc tgccaacatc ttggcaactt cctccactcg ctcttcgact gtcaagagac
     3061 gaacagtcga aaccgttgaa tggtcattac taatcttctc aataaagaat tgataatctg
     3121 caatcgcaat tacttgtggc aaatgggaga tagccaaaac ctgaccatgc tgaccaattt
     3181 tatgaatttt ctgagcaata gcttgagcaa cacgacctga aactcccgta tccacctcat
     3241 caaagacaat gctagtcttg ccttctttac gtgaaaaggc agacttaatg gctaacatga
     3301 gacgagataa ttcccctcca gaagcaacct taaccaaggg tttaaagtct tctccagggt
     3361 tggttgaaat ataaaactca accatttcat ttcccccacg actgaatttt cccttactaa
     3421 aatgaacctg aaactgggct ttttccatat aaagatcttg cagttcttgt ttaatctcag
     3481 cttcgagttg ctgagccaaa ttatgacgag cagaagcaag ttgacctgcc aaattgacaa
     3541 gattgacttc caacttctta agctctgctt ccatgtcctc agacgaaaga ttattgcctg
     3601 tcaagagatt gtattcttcc gtaatcttgg caaaataaag caaaacatca tcaacagtcc
     3661 caccatactt acgagtaata gtatgaagga ggtccaaacg attctcaacc tgcatcaggc
     3721 gattgccatc aaaatcaagg tcctcaatga tagcttccaa acgtttgcta atgtcttcta
     3781 aaacatagta ggtctcagac agagagcttg aaatttcacg gtattcagga tcatactctt
     3841 cgacactttc catgtcattc atagctgaac gaacattggc cagacttgaa aaatcttcat
     3901 tgtccaacat actgtaggca ttggtcagtg tatccgcaat atttttgtgg ttgaggagtt
     3961 tatctcgctc ttgattgaga gccaagtctt ctccagcctg caagtttgct gcctcaatct
     4021 ctgccatttg aaattccaac atttcgatac gttccttgtg ttcctgttgg tttttcttga
     4081 cttccagaac ctgcttgcgc attttccgat aggcatcaaa actcgtttga taggtttctt
     4141 tcaagtccca aaaagcggca tcaccaaatt catccaacat ctggatatgc agttggggac
     4201 gcattaactc ctcatggtca tgctgaccat gaatatctac aagatgttgc ccaatagctc
     4261 gcaaaacaga cagattaacc atctgaccat ttacacggct gatactacga ccattttgca
     4321 agatttcccg acggatgata atttcatcac ctaattctaa accttgctca tcaaaaattt
     4381 cctgtaaaag acgactattc tcaactgaga aaagcccctc aatctctgcc tttggtgcac
     4441 catgacgaat aacatctgtc gtcgcacgag ctcccaacat catattcatg gcatcaatga
     4501 taatcgactt ccctgcaccc gtttcaccag tcaggacagt catccccttt tcaaaattga
     4561 gggaaatagc ctcaataatg gcaaagtttt ttatcgaaat ttcaagtaac atatagacct
     4621 accaattttt tacttgttca aagatttcct ctgctagact tccacttctg gcaatgacta
     4681 aaatcgagct atcatcagtc aaacaactaa aaatcttgtc tgcaaaagta tcgattaact
     4741 gagcttttac aaaagccgta tttcctggaa taacttggag attgatcatc ttatccatca
     4801 attcagccga ttcgatattg tcttcagcca gttgcagact ttttacgatt gattttggta
     4861 attcgtagac ataggtgttg tctctcaaag gaattttgac aatacctaac tctttgatat
     4921 ctcgggatac cgtcgcctga gtggcagtga tacctgcttc tttcaaatgt tctacaattt
     4981 cttcttgcgt gccgatttga taatctgtca ccaatcttct aattttttca agtctctctt
     5041 ttttattcat ttttaaattg actatgcgcc ctctctactg cttctttaat ctcagcaaaa
     5101 atctgattgc ttgctgactt ttcttttttc aaatacgcta aaaattcaat atttccatgt
     5161 ccaccttgga tgggagaaaa gtccaagcca aggactgaaa aacctgcctc tactgccata
     5221 gctgttacag attcaaggac attctgatga atcttagcat ctcgaataat tccatttttc
     5281 ccaatctgct cacgtcctgc ctcaaactga ggtttgacaa gtgctaccac ctgaccttga
     5341 tcagccaaga cacggtgcaa ggctggcaaa atcagactaa gggaaatgaa actcacatca
     5401 atactggcaa agctcggctc ctgctcgaaa tcagtctttt cagcatagcg gaaattgaac
     5461 tgctccatgc taacaactcg tgggtcttgg cgtaatttcc aagccaactg attggtacca
     5521 acatcgactg caaagaccaa cttggcacta ttctgtagca tgacatcggt aaaacctcca
     5581 gtagaggccc cgatatcaat cgtagtcgcg ccatccaccg acaaatcaaa gacctgcaag
     5641 gccttttcca gtttcaaacc accacggctg acatacttga gtttctctcc cttgagtttt
     5701 aattcggtgt catctggaat tttctctcct ggcttgtcaa accgttctcc attaaggact
     5761 gctacgacta ggccagccat tacacctcgc ttggcctgct ctctcgtttc aaacaacccc
     5821 tgtttataag ctagtacatc cactctttcc ttagccattg attctcaaac tttctactac
     5881 acttacaatc gattctgttt caaagggaag ctgctgggca atttcttcta atttttcatt
     5941 agcttgatcc agggtttggt tacaaaaggc aatggactct tccaagccca acagggcagg
     6001 ataggttgat ttttctgcct gcagatcctt ttgaggtgtc ttgccgattt cctcaaaact
     6061 agctgtcaca tccagtacat catctctgac ttgaaaagca agtccaatca attcacccac
     6121 agttttcagc ttcacctgca tttcaggtga caattcagct ataatagctg ccgcttggaa
     6181 gggataggtt agtaacttcc cagtcttatt ggcatgaata atctgaagtt cttccaaaga
     6241 caagtgctgg tgttcgccct ccatatccaa aacttgccct gccaccatac ccagactacc
     6301 tgaagcaagg gataagttgg caatcaagtc caccttaatc tgacttggca aatctgcctg
     6361 cgcaatcaag gcatatgggt ctaagaataa ggcatctcca gccaaaatgg ccatagcttc
     6421 accgaatttc ttgtgattgg ttaaccgccc tcttcgataa tcgtcatcat ccatagcagg
     6481 aaggtcatcg tgaatcaagc tccctgtatg aatcatctcc aaggcagtag ctacctgcgc
     6541 gtgagcaggt ttgatggtaa cctgcaaggc ttccagaact tctaacaaga gaaaaggccg
     6601 aatacgcttg ccaccagcat gaatagaata gagaacagac tcccgtaaac tagaggcaaa
     6661 ctgctggtct ccataaaaat cttccaaagc cgactcgaca agagctaatt tttcttgctt
     6721 tttcattcaa aatcactttc tgttccgtct tcttgcatga ccttgaccaa ggtcttttca
     6781 gccttgtcca gcgtagcttg gagctctttt gacaagacca tgcccttttg aaaggcagta
     6841 atcgcatctt ccagagcaat ttcaccattt tccaaacttt ggacaatggt ttccagttct
     6901 gctagatttt cctcaaattt cttttgtttt gacatcttta acctctaatt ctacttgacc
     6961 atctcgcatc aaaagcgtta cttggtcttt tttcttcaaa ctctcaaccg aatctacaat
     7021 ggactcttct tttttgacaa tagcataacc acgcgccacg attcggctag tatccaacat
     7081 gagcaaagct tccgaaagtc gcttggcctc agcaaccttg gcgtcataaa ctaacgccat
     7141 ttggctacct aagagcttgt ccaactgtcc taaacggtct tgatagcgtt ggattttggt
     7201 aacaggtgat aattgtacta attgatgagt tcttgcttga actaattgtt tgttatcaga
     7261 aatccgagtt cgcaaacttt gtttcaaacg cagttgcagt tggtccaagc gttgcaaata
     7321 accgtcatac aagcgctcag gttgtctaaa gataacagac tgactgcatt ttttcaaagc
     7381 ctcttgtttc ttagatagaa catttcggac tgccgttgcc atccgttttt cctgattttg
     7441 caaatgagtt aatacatcca acttggtcac aggtgttgcc agttcagccg ccgctgttgg
     7501 cgttgcagcg cgtcgatctg ccacaaaatc tgccaaggtc acatccgtct catgccccac
     7561 actagagata actggcaaac gagattcaaa aatagctcgt accacaattt cttcgttaaa
     7621 ggcccagaga tcctcaatag aaccacctcc acgaccaata atgagcaaat ccaaatcgtc
     7681 ccgttgatta gcacgcgcaa tatttctagc aatttcctcc gcagcccctt caccttgaac
     7741 cttggtagga taaagaagga tgtcaacacc tgggaatcgc ctgctgacgg tcgtgataat
     7801 atctcgaata acggctccac tacggctggt tactacccca attctcttag aaaattgggg
     7861 tagagcttgc ttgaagcgtt cttgaaacag gcctttttct gtcaattttt tcttaagttg
     7921 ttcaaactga atcgcaagcg ccccaacccc atcaggctca gctttttcaa tgatgatgga
     7981 gtagctacca cttggttcat agacctgtac acgcccaatc acattgatct tcattccttc
     8041 ttccaggtca aaccctaatt tctgataaat cccagaccag atggtcgctt gaataactgc
     8101 atggtcatcc tttagggaga aatattggtg agtaggtcgt ttacgaaagt tggaaacttg
     8161 accagttaaa tagacccgtt ccaagtatgg gtctttatcg aatttcattt tcagatactt
     8221 ggtcaaagtt gttaccgata aatacttttc catctccacc tactattcat ttacttgctc
     8281 tttcatgggt attattatac caaaaatatg cctaaaaatc tccatttatg taccattatg
     8341 agggaaaaat agaaaaagga ggcaaggcct ccacatgtga ttatttgctg tttcgagctt
     8401 cttccaaaat ctttgcaatc ttggtcgtca acaggtcgat agccacggta ttgctaaccc
     8461 cttcaggaat gacgatatca gcataacgct tagttgactc gataaactgg tggtacattg
     8521 gtttgaccac acctaagtac tggttaataa cgctatcaag gctacggcca cgctcctcca
     8581 tatcacgctt gatacgacga ataatgcgca catcgtcatc cgtatccaca aaaatcttga
     8641 tatccatcaa atcgcgcaga cgcttgtcct ccaagaccaa aatgccctca acgataaaga
     8701 catcttgagg ctcctgacga taggtcttgc tactccgtgt atgctctgta tagtcgtagg
     8761 tcgggatgtc caccggacgc cctgccaaca attccttaat ctgctcgatc atcaagtctg
     8821 tatcaaaggc aaaaggatgg tcatagttgg ttttgacacg ctcttcaaag gtcaaatgag
     8881 actgatcctt gtagtatgaa tcatgctcaa tcatggaaat cttttcatca ggaaaatgcg
     8941 ataaaatggc tcttgaaaca ctagttttac caccaccaga accacctgtc actccaataa
     9001 tgattggtct attttgcatg ctatcctccg ttattttatc cgtcgtctat tctatcacat
     9061 tttttacaaa atttatagtc tcatcagcaa aaaacaccag agtcttccct ctgatgttcg
     9121 acagttttaa cgaaagccct gtagggtttg agcaagcatc tgcaagaaag ccagtctccc
     9181 tctttcctgg ctaggagctt tggcaatcca taaaccaaaa tcaatgacag acttaggtgc
     9241 ctggtaatga tgcgtcatga ggtaaagtga caacccttga ctcaaattgg ccatgaccgt
     9301 ttggtatttt tcatttctgt ctaaattttg tatagcaaga cgaaacaggt gactcaactc
     9361 actattttca tttatttctt tttgttgaag aagttgctcc accctttctc gaaactcatt
     9421 tcgcatcatg gaattatcac accaaatcct gggcctaagt tcccaccaga acctgtaatg
     9481 gactctaaat atttatctga aagaatttta aattgcttca tttcacacct ctaaatttct
     9541 atatagatta tttgagtacc tacaactcgc aacaattgga atattataat tcccaaaacc
     9601 attctactat tagtatgggg ttttatcaag caattattat ctatcacccg atttttattc
     9661 ctaaacgatc tagctaaaaa ccttccttgg cttatagaga ttgccccgtt tttctagaat
     9721 ggctaacaat ttatcatctt caaaggcagc caattctttg tccgtttggt ctagctcaat
     9781 aaaacgacca aagcgaactt ctgtagcctc ttctggagtt aggaaaactt tgacaaggtc
     9841 acctgtccca atctctaaag gatggagaaa atctaattgc ccagcctcta ctttttcagc
     9901 aatttcctcc aaggcaagag cgtcttctaa ttgtaagcca gcagcactag tacgagtcaa
     9961 atgggacata tgagccgcat aaccaagctt ttcacccaaa tcaactgaca aagtacggat
    10021 gtacgtccct ttactgcatt ttacacgaaa agtgaatcgg gcaagttggc catcataaga
    10081 aatcggactt gttcgctcaa attgataaat ggtcacctga cgttctggac gctccacttc
    10141 ctgaccagca cgcgcatact catagagctt gcgaccatta accttaactg ccgaatacat
    10201 agggggaatc tgagtaatag gcccagtcaa gctagcaatc gcttcatcaa caagcttttc
    10261 atccaagaga gacaaaacag gggtttctgc gaccacttcc ccactagcat cctcagtcgt
    10321 cgtggaatag cccagagtga tttccccctc atagatctta ccctcgtcct gcataaacac
    10381 gaccatgcgt gtcgccttgc caaccgcaat cggcaaaaca cccaccacat ccggatccaa
    10441 ggttccacca tgaccaattt tcttggttcc caaaatctta cgcagtttaa aaaccgcatc
    10501 atgcgaggtc attcctgctt ccttttttaa gttaataata ccgttcattt ttgctttcta
    10561 ctcccccttc agtgcttcga acttcgcttt cattgttgga tttttccggg tcaatttttt
    10621 aacagagaca tcttccaatt tgttgttagc tagacggagt tggttttcag atgtagtcag
    10681 gaatttctta acctcttcca tgcgtttgat ggccttgtcg atttcatcaa tagcttttcc
    10741 aaagttagtc gaagctgaat tatagttctt agcaaaagct agcttaaagg catccaaatc
    10801 ttcctcaaaa tgcgtaatgt caatattttg ctcgcgaacc aaggccaact cctgcttgta
    10861 ttttagggaa tttagcgccg cattacgtaa gagaccaatc aactggataa agaattgagg
    10921 acgaacaaca tacatttttt catactcgtg gctgacgtca acaatccctg tgttaaagta
    10981 gtcattatcg gcctcaagca tggtcaccaa aacggcatac tcacagttct tctcccgacg
    11041 gtccttgtcc aattccttgt aaaaatctgc attcttgtgc ttcttctctg ttccgtccgc
    11101 ttcgtttttc atctcaaaca tgatagaaat gatttcaact ccattttcat cacactcacg
    11161 gaagataaag tcccctttag acccacgcga agagaccttg ttatccttct caaagtaagc
    11221 atttggaaag gcgaaactac ggaccttgtt aaactcactc tctgcatact gttctaggct
    11281 ttccccaatc gcttttgtag attgttgagc cttaaaattc ttataaaact cgacttgttc
    11341 actggctgct ttgagctggg cttcgtagtt ttgcttaaca gaagccaaag acaactcatt
    11401 ttccttttct tgcagtagga gctgattttt gacctgattg cgttcttttt ctaggtcaga
    11461 aagggtcttt tgcagttgat tttcatgctc caaacgcaaa gtggccaatt ggttctccaa
    11521 agcctgtact tccttgtcct tagccaataa ggcctgatgg cttgtctgtt caacttcttt
    11581 cttggccaat tctttttctg tatcaaagtt ttggatttga ctctgcaatt gcgcaatttc
    11641 ttgatccttc tgagccagtt tagtctgttg ctcattcata gccttttgct cagccaaggc
    11701 cagttcctgc ctcatcctat cgtgtagttc cttatcaaac tctgccgttc tcacttggga
    11761 caaaagttca gcatactggc tttcatttac tgtaaagact tccccacagt tgggacattt
    11821 gatttcgttc attacattcc tctttctaga cttctgtaac cattatatca tgcctgaggg
    11881 aaaatcaaaa acagtgagtc aaaacccact gtttatcttc ttttacttta aattttttcc
    11941 aaggccaagc gtaagtcttc aatcaaatct tctacatttt caagaccgat tgacagacga
    12001 atttggtttg gtgtgacacc tgctgcttct aggtcttttt ctgacaattg accgtgagtg
    12061 gttgttgctg gatggacaac aagcgattta gcatctgccg cgtttgcaag gtcagaaaag
    12121 atttctaaat tatcaatgac cttgcgtgct tcttcctcgt cacctttgac gtggaaggta
    12181 aagattgaac cgacaccttt tggcaagtat ttctcagcca aggcatgata aggactatct
    12241 gcaagttttg gataatttac cttttctacc ttaggatggt tgacaagaaa atcaacaatt
    12301 gtctcagcat tttgtacatg gcgttccaca cgaagtgaaa gagtttcaag tctttgtagc
    12361 aagaggaaag cattgaatgg tgacaagact gcacctgtat cacgaagcaa ttgaactcga
    12421 acagctataa taaaggttgc tgcacccaca tcacgaatat agctcaaatt gtggcagctt
    12481 gaaccctcgt caacaaattg agggaatttc cctgaagccg tccagtcaaa acgaccacta
    12541 tcgacaatta ttcctccaat agttgtacca tgcccaccga taaacttagt cgcagagtga
    12601 atggcaatgt caacgccatg agagaagacg ttaatcaaat aaggtattgc aaaagtattg
    12661 tctgacacaa gtgggatttg atgtttatga gcaatctctg ccagtttttc caggtctgga
    12721 atattaatca aggggttacc caaggtttca atcaagacaa gcttggtatt gtctttgata
    12781 gctgcttcta cttcctccaa attatcaaca tcgacaaagg ttgttgtgat accataacga
    12841 ggaaggggtt ctttcaaaag attgaaggtt ccaccgtaaa tagtcgaagc agccactaca
    12901 tggtcaccag catgggcaag cgccaaaatc gtataagtca ctgcagtcat acctgatgct
    12961 gttgctagcg cttcaacacc accttcaagg gcagctgttg taggattggt gatacgagta
    13021 taaatgttcc ctggtttcct caaggcaaac agatcggcac cttcctacgt gtcatcaaaa
    13081 acaaaaaatg ttgtttgata aatcggcact gcacgagact tagtagctgg agccacaact
    13141 tgaccagcat gtagttgtag agtttcagat ttaaaatcac aagtcataag gtgacctcca
    13201 tatcatttta ttaaggatat tgtagcacct tgagttatag tttacaaata
//
LOCUS       DAVLSL010000041        13234 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLSL010000041 DAVLSL010000000
VERSION     DAVLSL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.042"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            128..2293
                     /locus_tag="VXN30_000971"
     CDS             128..2293
                     /locus_tag="VXN30_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1357617.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2293..3033
                     /locus_tag="VXN30_000972"
     CDS             2293..3033
                     /locus_tag="VXN30_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1357618.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3182..4669
                     /locus_tag="VXN30_000973"
     CDS             3182..4669
                     /locus_tag="VXN30_000973"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1357619.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTAATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(4715..6004)
                     /gene="ftsY"
                     /locus_tag="VXN30_000974"
     CDS             complement(4715..6004)
                     /gene="ftsY"
                     /locus_tag="VXN30_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1357620.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVDEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDSEPVVGVSQEEVEEFPHSEELPEEEKLDHE
                     GTIEENNSEVLEAERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVLEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(6008..6826)
                     /locus_tag="VXN30_000975"
     CDS             complement(6008..6826)
                     /locus_tag="VXN30_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1357621.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6826..7620)
                     /locus_tag="VXN30_000976"
     CDS             complement(6826..7620)
                     /locus_tag="VXN30_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1357622.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLGKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            complement(7617..11156)
                     /gene="smc"
                     /locus_tag="VXN30_000977"
     CDS             complement(7617..11156)
                     /gene="smc"
                     /locus_tag="VXN30_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1357623.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALDIALGASSQHIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEKDRLSEGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVTDIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11147..11845)
                     /locus_tag="VXN30_000978"
     CDS             complement(11147..11845)
                     /locus_tag="VXN30_000978"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1357624.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(12028..12099)
                     /locus_tag="VXN30_000979"
     tRNA            complement(12028..12099)
                     /locus_tag="VXN30_000979"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12064..12066),aa:Arg,seq:ccg)
     gene            complement(12163..13149)
                     /locus_tag="VXN30_000980"
     CDS             complement(12163..13149)
                     /locus_tag="VXN30_000980"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1357625.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
BASE COUNT         3752 a         3019 c         2451 g         4012 t
ORIGIN      
        1 ctttttaata gcaattcaag gaaaggaaaa aatcgatgtt atattttctg acacgaaaaa
       61 tataaaaatt tgcctttttt tactttttct gatataatga gagaatattc ggaaaaggag
      121 actaaaaatg aagaaaaaat ttctagcatt tttgctaatt ttattcccaa ttttctcatt
      181 aggtattgcc aaagcagaaa cgattaagat tgtttctgat accgcctatg caccttttga
      241 gtttaaagat tcagatcaaa cttataaagg aattgatgtt gacattatta acaaagtcgc
      301 tgagattaaa ggctggaaca ttcagatgtc ctatcctgga tttgacgcag cagtcaatgc
      361 ggttcaagct gggcaagccg acgctatcat ggcagggatg acaaagacta aagaacgtga
      421 aaaagtcttc accatgtctg atacttacta tgatacaaaa gttgtcattg ctactacaaa
      481 gtcacacaaa attagcaagt acgaccaatt aactggcaaa accgttggtg ttaaaaacgg
      541 aactgccgct caacgtttcc ttgaaacaat caaagataaa tacggcttta ctattaaaac
      601 atttgacact ggtgatttaa tgaacaacag cttgagtgct ggtgccatcg atgccatgat
      661 ggatgacaaa cctgttatcg aatatgccat taaccaaggt caagacctcc atattgaaat
      721 ggatggtgaa gctgtaggaa gttttgcttt cggtgtgaaa aaaggaagta aatacgagca
      781 cctggttact gaatttaacc aagccttgtc tgaaatgaaa aaagatggta gtcttgataa
      841 aattatcaag aaatggactg cttcatcatc ttcagcagtg ccaactacaa ctactctcgc
      901 aggattaaaa gccattcctg ttaaggctaa atatatcatt gccagcgatt cttcttttgc
      961 accttttgtt ttccaaaatt caagcaacca atacactggt attgatatgg aattgattaa
     1021 ggcaatcgct aaagaccaag gttttgaaat tgaaatcacc aaccctggtt ttgatgctgc
     1081 tatcagtgct gtccaagctg gtcaagccga tggtatcatc gctggtatgt ctgtcacaga
     1141 tgctcgtaag gcaacttttg acttctcaga atcatactac actgctaata ccattcttgg
     1201 tgtcaaagaa tcaagcaata ttgcttctta tgaagatcta aaaggaaaga cagtcggtgt
     1261 taaaaacgga actgcttctc aaaccttcct aacagaaaat caaagcaaat acggctacaa
     1321 aatcaaaacc tttgctgatg gttcttcaat gtatgacagt ttaaatactg gtgccattga
     1381 tgccgttatg gatgatgaac ctgttctcaa atattctatc agccaaggtc aaaaattgaa
     1441 aactccaatc tctggaactc caatcggtga aacagccttt gccgttaaaa aaggagcaaa
     1501 tccagaactg attgaaatgt tcaacaacgg acttgcaaac cttaaagcaa acggtgaatt
     1561 ccaaaagatt cttgacaaat acctagctag cgaatcttca actgcttcaa caagtactgt
     1621 tgacgaaaca acgctctggg gcttgcttca aaacaactac aaacaactcc ttagcggtct
     1681 tggtatcact cttgctctag ctcttatctc atttgctatt gccattgtca tcggaattat
     1741 cttcggtatg tttagcgtta gcccatacaa atctcttcgc gtcatctctg agattttcgt
     1801 tgacgttatt cgtggtattc cattgatgat tcttgcagcc ttcatcttct ggggaattcc
     1861 aaacttcatc gagtctatca caggccaaca aagcccaatt aacgactttg tagctggaac
     1921 aattgccctc tcactcaatg cggctgctta tatcgctgaa atcgttcgtg gtggtattca
     1981 ggccgttcca gttggccaaa tggaagccag ccgaagcttg ggtatctctt atggaaaaac
     2041 catgcgtaag attatcttgc cacaagcaac taaattgatg ttgccaaact ttgtcaacca
     2101 attcgttatc gctcttaaag atacaactat cgtatctgct atcggtttgg ttgaactctt
     2161 ccaaactggt aagattatca ttgctcgtaa ctaccaaagt ttcaagatgt atgcaatcct
     2221 tgctatcttc tatcttgtaa ttatcacact tttgactaga ctagcgaaac gcttagaaaa
     2281 gaggattcgt taatggcaaa attaaaaatt gatgtaaatg atttacacaa gcactatgga
     2341 aaaaatgaag tcctaaaagg aattacgact aagttctatg aaggagatgt tgtttgtatc
     2401 atcggtcctt caggttctgg taagtcaact ttcctccgta gcctcaatct tttagaagaa
     2461 gtcactagcg gtcacatcac tgtgaacggc tatgatttaa ctgaaaaaac aaccaatgtt
     2521 gaccacgtcc gtgaaaatat cggcatggta ttccaacact tcaacctctt ccctcatatg
     2581 tctgtattgg acaacatcac ctttgctcct attgagcaca agttgatgac taaggaagaa
     2641 gctgaggaat tgggaatgga gttgcttgaa aaggttggac tagcagataa agctaatgcc
     2701 aatccagata gcctatcagg tggtcaaaaa caacgtgtgg ccatcgctcg tggcctagca
     2761 atgaatccag acatcatgct cttcgatgaa ccaacttctg cccttgaccc tgagatggtt
     2821 ggagacgtac ttaacgttat gaaggaattg gctgagcaag gcatgaccat gattatcgta
     2881 acccatgaga tgggatttgc tcgtcaggtt gccaaccgcg ttatctttac tgcagatggc
     2941 gagttccttg aagacggaac acctgaccaa atctttgata acccacaaca ccctcgtctg
     3001 aaagagttct tagataaggt cttaaacgtc taaactcaaa ctgtaaggat ttccttgcag
     3061 tttttctacc tcgtattgga atttttgatt tttcggaaaa ttatgttaga attaagttta
     3121 tgaaatgagg tttcctcata cctagcaaga ctaggaataa aaatagaaat taggtagcta
     3181 gatgtcatct aaggttattg ttacaatttt cggtgcgagc ggagacctgg ctaaacgcaa
     3241 gctctaccct tcccttttta gactatataa atccggcaat ctttccaagc actttgccgt
     3301 tattggaact gcccgtagac cttggagtaa ggaatatttt gaatctgtag ttgtcgagtc
     3361 catccttgat ttggcagata gtaccgagca agcccaagaa tttgctagcc acttctacta
     3421 tcaaagccat gatgtcaatg attcggaaca ttatattgct ttgcgtcaat tacaagctga
     3481 gcttaatgaa aaataccaag ctgaacacaa taagctcttc ttcttgtcta tggcacctca
     3541 gttctttgga accattgcca aacacctcaa atctgaaaac attgtcgatg gcaaaggttt
     3601 tgagcgcttg atcgttgaaa aaccatttgg tacagattac gcaactgcaa gcaagttgaa
     3661 tgacgaactc ctagcaacat ttgacgaaga acaaattttc cgtatcgacc attatcttgg
     3721 taaggaaatg atccaaagca tctttgcagt tcgctttgca aacttgattt ttgaaaacgt
     3781 ttggaacaag gattttatcg acaatgttca aattaccttt gcggagcgct tgggtgtaga
     3841 agaacgtggt ggctactatg accaatccgg tgccctccgt gacatggtcc aaaaccacac
     3901 tctacaactt ctttcgctcc tcgccatgga caaaccagca agcttcacaa aagacgagat
     3961 tcgtgctgaa aagattaagg tctttaaaaa cctctatcat ccaactgatg aagaactcaa
     4021 agaacacttt atccgtgggc aataccgctc tggtaagatt gatggcatga aatacatctc
     4081 ttatcgtagc gagccaaatg tgaatccaga atcaacaact gaaaccttta catctggtgc
     4141 cttctttgta gacagcgatc gattccgtag tgttcctttc tttttccgta caggtaaacg
     4201 actgactgaa aaaggaactc atgtcaacat cgtctttaaa caaatggatt ctatatttgg
     4261 agaaccactt gctccaaata ttttgaccat ctatattcaa ccaacagaag gcttctctct
     4321 tagcctaaat gggaagcaag taggagaaga atttaacttg gctcctaact cacttgatta
     4381 ccgtacagct gcgactgcaa ctggtgcttc tccagaacca tacgaaaaat tgatttatga
     4441 tgtcctaaat aacaactcaa ctaactttag ccactgggat gaagttggtg cgtcatggaa
     4501 gttaattgac cgtattgaaa agctctgggc tgaaaatggt gccccacttc atgactataa
     4561 agctggaagc atgggacctc aagccagctt tgacctactt gaaaaattcg gtgccaaatg
     4621 gacttggcaa ccagatatcg cctatcgtca agatggtcgc ttagaataaa aaaatttcct
     4681 gcaagtttat gccttgcagg atttttgctt ctgattagat taaaccttcc aagagacctt
     4741 tcataaagtt ttctgagtta aactctccaa tatcatcgat tttttcacca aaaccaatca
     4801 attttacagg aatattgagt tcttcacgaa tggctagaac cacacctcct cgagcagttc
     4861 catcaatctt agtcaaaaca attccggtta aaggtgtgat tttcgaaaat tctttggcct
     4921 gtactagggc attttgacct gttgatgcat caagtgccaa gaaggtttca tgtggtgctt
     4981 ctagcacaac acgtttgata atacgaccaa tcttttccaa ctcagccata aggttatcct
     5041 tattttgcag acgaccagca gtatcaatca tgagaatatc gataccttca gccacggcac
     5101 gttccatacc atcaaagacc acgctggctg gatcagcttt ttcaggtcca gttactactg
     5161 gaacatctac tcgtcggccc cattcagcta gctgagctac tgcacccgca cggaaggtat
     5221 ctgctgcaac cagcatgacc ttcttaccag ctcgtttgta gcggtgggct agttttccga
     5281 tagaagttgt tttcccaaca ccattcacac caacaaagag cataactgtc aagttatctt
     5341 ggaagtggat gctttcatcg tagctaccat ccttttcata aagctcaacc aatttctcaa
     5401 tgatgacacg acgaagtgca tcaggtttct tggcattttc aagcttggct tcgtaacgta
     5461 gttcctccgt taagttagaa gcgacttgga caccaacatc actcataatc agcagttctt
     5521 ccagttcctc gaaaaattct tcgtcaacag agcggaagtt agcaaagaag gcattcaagc
     5581 gggcaccgaa acctgtgcga gttttcttaa gactgcggtc atatttttcc tgaacggttt
     5641 cttctgcttg aggcctttct gcttcaagaa cttcagaatt attttcttct atagttcctt
     5701 cgtgatcaag cttctcttcc tctggtaatt cttctgagtg tggaaattct tcgacttctt
     5761 cttgagaaac ccctacaact ggctctgaat ccagactttc ttcaactgtg tcttggattt
     5821 cctcttcttg gaacacagct tgttcaacaa tttcaacctc tgcttcttcc tgaggaactt
     5881 catcaacttc tgtgaaggta ggatcaacat cttcagacaa atcaagattt tccagagctt
     5941 cttttacaac ttcttcgatt ttaggttctt ctttttttcc gaatagacgg tcaaacaatc
     6001 ccatatctta gttctccttt agcacatatt cttcgatagc ccaggcgaca gcttcctcat
     6061 cgttggtcat aggagttacc acatttgcga ctgccttgac ttcaggaaca gcgttttgca
     6121 tggcaacacc gagccctgcc cattcaatca tagagaggtc attggcctcg tcaccacagg
     6181 ccataacttg gctttggttg attccaagat ggctaattag ttttgccaaa cctgttgctt
     6241 tatgaacatt ctttggtgac cactctagca acatttcacg tgatttaaag atttcatatt
     6301 ggtcaaacaa ttctggagaa atcttctgaa tggctgcatc caagggttct tgagcaaagg
     6361 cagtcacgca tttgttgtag gtcatttgac tagataagtc ttcaaagtcc actggaacaa
     6421 aggtcaaggc tggattgaat ttggcataaa gactttcttg gtccgattgg atttgataaa
     6481 ctgttccttc tgagatggca tcaagaggca gtgataattt ctctgtttct tcatacaaac
     6541 gtgccacatc atcatatgaa aagactgttt tatcaaggat ttctcctgta tttttctgaa
     6601 ctaatccacc attaaaagta atggtatact catcttcctg accgtcagtc cctaactcat
     6661 ggagaaagaa atccatggct tttaagggac gaccagttgt caatacgacc ttgataccac
     6721 gatcacgcgc agctttcaag gtttccttgg tacgatccgt cagcctttta tcagtagtca
     6781 gcaaggtccc gtccaagtcc aatgcaatca attttatatc tgccattata agccctccat
     6841 ataagctata accgaccgtt ccttatggtg accaatcaca gtctttgcta attctaaaat
     6901 ttcaggtcgt gcattttcag gagctacagg atgtcccaca acctgcatca tatgtaagtc
     6961 attaagattg tctccaaaag ccatgacctg atccattgtg ataccaagtt ttttagctaa
     7021 ttcaacaata gctacaccct tatcgacata gtccagaaca atatcaatag attcaaagcc
     7081 agttgtcata gccttgacac cagggacatt atcattgacc caagcttcac cagcttctaa
     7141 cgtttcttct gtgaagttgg ttgtaaattt gaaaatgtca tctgtgatat cttccaaact
     7201 cgctactttt tggatatttt cattataatg ctgactcact ttcaaatagg tctcatcaac
     7261 cgtatctaga acatatgacc ccttcttacc cgtcaagagc agtttattga tatctacata
     7321 aggtgacgtt ttcagctttt caaaagttgc cagataaaag tcacgagaca tagtcgcttc
     7381 atacaagtcc tgaccttgat actctaccaa actgccattt tccgcgatga aaataatgtc
     7441 atcacgaaca ccagcaaata attttcctag agacagaaat ccccgacccg aagccaccgc
     7501 aaagtaaatc cctttttcct tgtaggaaac caagagagac ttgagacgat ccatatcaaa
     7561 gcgtccattc ccatctagga aggttccgtc catatccgtt gctactagtt taattgtcat
     7621 ccttcaatac tttctaaatc ttttaactta actgaaacaa tctttgaaac acccgattct
     7681 tgcatggtca ctccatagat agaatcggcc gctgccatgg tccccttacg gtgggttacg
     7741 acaataaatt gactgtcctt atcaaagcgg ttgagataat ccccgaaacg tttaacattg
     7801 gcttcgtcta gcgcagcctc tacctcatcc aagataacaa acgggatagt cttaactcga
     7861 atgatagaga agagcaaggc aagagccgat agggcttttt caccaccact catgagatta
     7921 agagactgga ttttcttgcc tggtggttgg acagaaattt caaccccagc tgtcagcaag
     7981 tctccttcag tcaaaatgag gtcagcctga cctccaccaa acatctgctt gaaggtcact
     8041 ttaaaggact cacgaatggc ctcaaaggtt gatttaaagc gttccttgac ctcatcattc
     8101 atctctgtga tggtttcaag aagcaggttt ttcgcagaca aaatatcatc acgttggcta
     8161 tttaggaaat ccagacggtt gtgaacttct tcgtactggt caatagcttc tatattgaca
     8221 ggacccagtg agcgtatagc cttctctaaa tccttaactt cttgctctgc cagattgaga
     8281 ttttccaact catgcgcctt ttctaaagct tctgtgtagc tgatctggta ctggtctgtt
     8341 aattgacttt gtagatggcg caagcgctcg ctaacctttt ctttctttgc ttcagcacga
     8401 gtttgcttgc gaatccactc ttcattctgc tggcgagcct gatccaaatg actagcaata
     8461 tcatccagtt gaccctcaat atcatccaac tcaaactgct tgcgaatcaa accttgttgg
     8521 agatttgttt tttgagtttt ggcttcatca gcctgttgac tgagcaaatc cgtatcaact
     8581 ttctcaagat tgtcaacctt ttcttgaaga aggcgctgaa tttcttcttg ctcaatatca
     8641 agattatcta gttccttacc taagcgttca atatcagtca cttcataacg tttttgccct
     8701 tgcagttctg tcttaagcaa acgagcttgc gctagctctt cctgcaagtt ttgatagcgt
     8761 tcttggatgg catttttgtt agacttaatc tcttcaatct cagcttccag attttgcttg
     8821 tcactggcga ttgcagcaag acgctcttgg cagttttcct tgtctgcttg ccaatctccc
     8881 tcggaaagac gatctttttc ctcttcttgg agtttccaaa gagtttccag ttcttcaact
     8941 tgctgactag tttgctgata agcgaggaac aagccttgct cctgaatacg tgcctgctct
     9001 ccttgagatt tgatggcttc taatgactcg gtcaatctgg ccatctcatc ttgcaaggtc
     9061 ttcaaagcca cttcttctga acgcaagctt gcttcatctg cagcaatttc tttttgtaat
     9121 tgctccagtt ctggcttgat gaaaatactg ttattttggc gattggcacc acccgcatag
     9181 gaaccacccg tgcgtaattc tgtcccatcc aatgtcacca tacgaacctg ataacgaact
     9241 tggcgagctg ctgcacgcgc atgttctacg gtatcaaaga tagccgtcgt agctagcaag
     9301 ttcttgaaaa tggcttccag tctagtatca aaagtcacca actcatctgc catcccaagg
     9361 aaacctgggc ttacagcgat agcatcttgg ttctgactag aaatcgtacg cgccttaata
     9421 gtggtcaaag gaagaaaggt tgcacgaccg gctctgtttc gtttgaggaa atcaatggct
     9481 ttggttgccg cattctcatc ttctacaata atgtgctgac tactcgctcc aagcgcaatg
     9541 tctagggcag tttgataatg catatcaaag gtcagatgct cactgactgc accaataatc
     9601 ccacctaggc gatctttttc ttggagaaca ctcttaacac ctgcataaaa gttactatga
     9661 tttctcagga tattttccaa actttgagct ctggcctgct tgtttttgag attatccaga
     9721 cggtcaaaga gttggctttg ttgagcttga taggaagttt tctgctcctc ttgctccttg
     9781 gcaatagctt ggtagtcagc caataatttc tgaacctgct ccttggcagt ttcaagctct
     9841 tccttttgct gactagcctt ctctttagct atagctaatt gctctttcag cttttctagt
     9901 tgatctgctt gtttttgaga aagctgacga ctattttcca actcattctc aatacgggtc
     9961 aactggtttg agacatccgc ttcttcttgt aaaagagcta caaagcgttc acgtaagagc
    10021 tcaatcatct gatcaggatc gtctgagaaa gccagcaatt cagcttctaa acgattgagt
    10081 ttttgattat tttggactag atttccctct aacagagcta aagagctttc tttatcagac
    10141 ttttctttgc tgagtgaatt tctcttatcc tccaaagcag ccaaacgagc ttgtgcctcc
    10201 tgttgattca aggccacttg ctcggactcc agtttcgata gggctaattt tctttctaaa
    10261 tcactaatca gactagtcaa gtccatcaaa ctgccttggt ctttggccat ttcagcctgt
    10321 aaatcttggc gttgcttttt aagagtttga ttttcttctt ctaatttttc acgcttttgg
    10381 taataactca tcaagagttc ttgaacctga gccaactctt cttctgtcga ctctagttct
    10441 gccttatttt ccttgatttg agcaaccaat acatccaagt aaatagcctt gcgttgacct
    10501 tctaagtcta gaaatttacg ggcattttca gcttgcttct caagaggctt gatttgatta
    10561 tccaactcgt agataatgtc ctctaagcgg tccagattat cctgagtttg ctgcagttta
    10621 ctctcggttt cttttctgcg agtcttgtat tttaaaactc cagcagcttc ttcaaaaata
    10681 gctcgtcgct cctcaggctt ggaattaaaa atctcctcaa ccttcccttg ggaaataata
    10741 gagaaggaat ctcgtcccaa acctgtatcc aagaaaaggt catgaatatc acgcagacgg
    10801 actttcttgc cgtcaatctt gtattcgcta tctccactac gatagatatg gcgttccacc
    10861 ctgatttctt gacctgcatc cttgataaat ccgtcatgat tatccagagt cacaactaca
    10921 gaagcataat tgagcggttt gcgactttct gttccagcaa agatgacatc cggcatcttg
    10981 cccccacgga gactcttgac actagactcc cccaaagccc aacgcagact ttctgtaata
    11041 ttggactttc cagatccatt gggtccaaca actgccgtca caccttggtc aaaaacgacc
    11101 ttggtcttat cagcaaaaga cttgaacccc tgaatttcga tttcctttaa atacatgaat
    11161 ccagcccctt ctcaacggca tttttggcag cttcctgctc tgctaatttc ttagaacgac
    11221 cttggccttg accgatgctc ttaccttcaa caagaacttc tacatcaaag accttatcgt
    11281 gagcaggccc tgtttcagaa atcacctgat aacgaatagc cacatcacca ttgacctgaa
    11341 gtaactcttg gagatgggtt ttatagtctg taatcatctc aaattcgcct gcttcaacct
    11401 taggaatcat gacttgatag ataaattcct tgaccttggc cacatcctta tccaaaagaa
    11461 gggcaccaag aaaggcttca aaggcatcac caagaatggt gtcacgattg cgaccaccag
    11521 atttttcttc ccctttaccc aacttgataa actggtcaaa ctggcaatca cgcgcaaaac
    11581 cagctaaact ctcctcacgg acaatcatag cacggagttt tgataggtca ccttcaggct
    11641 ttttaggata ttttttatac agatattctg aaatcaataa ctgtagaaca gcgtctccta
    11701 aaaattccaa gcgttcatta tgtgaaattt ttaagaggcg gtgctcattg gcataactcg
    11761 tatgagtaaa ggcagtttcc agtaactttt tgtctgtaaa ttcgattgca aaatggttct
    11821 ttagtacagt ttgtaattct ttcataccaa cctctttcta actgataaca gtccttttta
    11881 ttatatcaaa aaaagccccc tgagtcactc taaaacggga ctggaaagca tttgggaatt
    11941 ctttagacag agattctcag ttttagtggc aaatttagag tagtgtaaag aaaaaagccc
    12001 tattaaaggc tttttagaat gtttacatcc accctgaggg aatcgaaccc ccatctcaag
    12061 aaccggaatc ttacgtgata tccattacac taagggtgga aacttgtttt attataacag
    12121 aaatttgctc taataacaag ttttttagtc aaagaccccg tcttagtggg aagcatcccc
    12181 attccagatg gagtttttca cgatcacata atcaacgtgt ttaaggtcag caacctgacg
    12241 tccacctgca taagaaatag cactttgaag gtcttgttcc atctcagtta aagtgtcttg
    12301 cagatgacct ttagcaggaa gcaagatacg tttgccttcc acatttttgt aagcaccttt
    12361 ttgatattgt gaggctgaac cataatattc tttgaactgt tcaccatcga cttcaatcgt
    12421 tttccctgga ctttcaatat gtcctgcaaa gagggaacca atcatgatca tgctagcacc
    12481 gaagcggata gacttggcaa tatcaccgtg agtacgaatt cctccatcag cgataatcgg
    12541 tttacgcgca gccttggcac accagcgtag agcagccaac tgccaaccac ctgtaccaaa
    12601 accagtctta accttggtga tacaaacctt accaggaccg attccgacct tagtagcatc
    12661 cgcaccagca ttttccaatt cacgcacagc ttctggtgtt cccacatttc cagcaatgac
    12721 aaaggtatct ggcaattctt tcttgatgtg ttgaatcata gaaatcacgc tatccgcatg
    12781 accatgagca atatcaatag tgatatactc aggagcatca gtcttgagct gtctaacgaa
    12841 atcatactca tagtccttaa caccgacaga tatggaagca atgagccctt gattgtgcat
    12901 tcgtttaata aaaggaatgc gtcctgcctc atcaaaacgg tgcataatgt agaagtaacc
    12961 acctttagcc agttgctctg ctacattttc atccaaaatc gtctgcatat tcgctggcac
    13021 aacaggtagt ttaaaggtgt gatttcctaa agtgacactt gtatccgctt ctgcacggct
    13081 tttaatgaca catttatttg gaatcaattg aatatcttcg taatcaaaaa ttggaaattc
    13141 atttaacata tcgatgtctc gtttcttttg taatgaccta cctatgctct tgcatcacta
    13201 cgccttttcc gacgtttcct taattcatta taaa
//
LOCUS       DAVLSL010000042        13202 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLSL010000042 DAVLSL010000000
VERSION     DAVLSL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.043"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            122..298
                     /locus_tag="VXN30_000981"
                     /pseudo
     CDS             122..298
                     /locus_tag="VXN30_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            632..772
                     /locus_tag="VXN30_000982"
     CDS             632..772
                     /locus_tag="VXN30_000982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357626.1"
                     /translation="MIDATEVQINRPKKELANDSGKKKCHAMKAQAIVTSQGRLFLWI
                     SL"
     gene            802..1101
                     /locus_tag="VXN30_000983"
     CDS             802..1101
                     /locus_tag="VXN30_000983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1357627.1"
                     /translation="MSRRNIGQAGKILADSGYQGLMKIYPQVQTPRKSSKLKPLTAEN
                     KAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(1264..1515)
                     /locus_tag="VXN30_000984"
     CDS             complement(1264..1515)
                     /locus_tag="VXN30_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357628.1"
                     /translation="MPKTKTLAELADVILWSFDFVNDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYIENVYLDSLSVKQKFKFIFDFGDEWRFEC"
     gene            complement(1892..3772)
                     /locus_tag="VXN30_000985"
     CDS             complement(1892..3772)
                     /locus_tag="VXN30_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1357629.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEILALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3766..4635)
                     /locus_tag="VXN30_000986"
     CDS             complement(3766..4635)
                     /locus_tag="VXN30_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1357630.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSHQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4719..7364)
                     /locus_tag="VXN30_000987"
     CDS             complement(4719..7364)
                     /locus_tag="VXN30_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1357631.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVESALPEQL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLDPQEIRTEFIKKEEI"
     gene            complement(7455..8735)
                     /locus_tag="VXN30_000988"
     CDS             complement(7455..8735)
                     /locus_tag="VXN30_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1357632.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLSYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8906..10990
                     /locus_tag="VXN30_000989"
     CDS             8906..10990
                     /locus_tag="VXN30_000989"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1357633.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDMATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11030..12709)
                     /locus_tag="VXN30_000990"
     CDS             complement(11030..12709)
                     /locus_tag="VXN30_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1357634.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGSLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3692 a         3131 c         2680 g         3699 t
ORIGIN      
        1 gaaattaaag tgggttaaaa aagaaattaa ctgagtaaaa actctcgaaa ttcaattcgc
       61 tatattatca ctcaacttga acaaatacca gaaccacaaa aaacagatgt aaaggaaaaa
      121 tatggactta aaatttgaag gggttgattt agaatacaaa aaagctaaaa acaatcttcc
      181 agagtctttc tgggaaacct actcagcatt tgcaaataca aatggcggga aaataattct
      241 cgggatagat gaaaaaaaca ttgacaccta ccaaagagtc aatagactcc ctgcgaaaca
      301 aaattatgaa gcaagtaaac aactaactga tgcacgattt aagcgtcttg ttggtgttca
      361 gcgcacgact tttgaagaga tgttagctgt attaaaaaca gcttatcaac ttaaacacgc
      421 aaaaggtgga cgaaaaccta aattaagcct agaagacctt cttatggcca ctcttcaata
      481 tgtgcgagaa tatcgaactt atgaagaaat tgcggctgat tttggtatcc acgaaagcaa
      541 cttactccgt cggagctaat gggttgaagt aactcttgtt caaagtggtt ttacgatttc
      601 aagaactcct ctcagttctg aggacacggt aatgattgat gcgacggaag tacaaatcaa
      661 tcgccctaaa aaagaattag cgaatgattc tggtaaaaag aaatgccacg ctatgaaggc
      721 tcaagcgatt gtcacaagtc aagggagatt gtttctttgg atatcactgt gaactattgt
      781 catgatatga agttgttcaa aatgagtcgc agaaatatcg gacaagctgg taaaatcttg
      841 gctgacagtg gttatcaagg gctcatgaag atatatcctc aagtacaaac tccacgtaaa
      901 tccagcaaac tcaagccgct aacagctgaa aataaagcct ataaccatgc gctatctaag
      961 gaaagaagca aggttgagaa catctttgcc aaagtaaaaa cgtttaaaat attttcaaca
     1021 acctatcgaa atcatcgtaa acgcttcgga ttacgaatga atttgagtgc tggtattatc
     1081 aatcatgaac taggattcta gttttgcagg aagtctattg aaatactaag cctacacaga
     1141 ctgatttcaa ttttaccatt cttcatagtc aaaaccatca taatctggat attgttctgg
     1201 caacgttcca accgaacgta cgagataagc ttcttcgtcc tctgtctcga tttctctcag
     1261 cacttagcat tcaaaacgcc attcatcacc gaagtcgaaa ataaacttaa atttttgttt
     1321 gacacttagg ctatccagat agacattttc tatgtaacgt tcttcaacat cgtcactaac
     1381 aaagctaaga aagtaagaat ctgcatgact ccactcaaca ttatccatga aaaatgcatg
     1441 agcatgatca tttacaaaat cgaaactcca taaaatcaca tctgctagtt ccgctaacgt
     1501 ttttgttttt ggaatctata tatgtcggta gcaacctttt ttaatttgac ggaaatgatc
     1561 caagcttcta cagatgctgg cttttcagct tctatttctt gttttactaa ttttaagtgt
     1621 agcataatac ctccaattgc tcatcgatag aattgctctt aggaaacatt cactttctgc
     1681 tattttatca taagtttgtc taactgtagc atttgaaacc agataattct tctgtttcac
     1741 gcgaaacacc ctgcgccaag caaggtgttc ttgtttttat actcaatgaa aatcaaagag
     1801 caaactagga agctagctgc aggctgctca aaacactgtt ttgaggttgt agatggaagc
     1861 tgacgtggtt tgaagagatt ttcgaagagt attaagtcgt ataaatcgtc gacgctgtcg
     1921 caatggtatg ccactggtta gctgtagttg ctgcgaagtc cttgtctgcg tagaagtcag
     1981 taaatggtag gatttcaact tccagctcgt cgatgcggtc aagctgacca gccagataaa
     2041 cctcgatacg gctttctgct cgtttgatgc gttgcaagag tccgcccata cggatgtcaa
     2101 ctgtatccaa accaaagacc ttgttttctt tcagccattg gtggctaaag agggcatgga
     2161 agtcttcaat ttggcttcta agttctggta attcttgtct ggcgatttgt tgtaaacttt
     2221 ctttatcatc cgcttggtag gcctgacgaa tgcgtcgtcc cacatctact ttgctactta
     2281 aaatagcatt caactgggcc tgagtttcaa agagataagc atagtttcca gctttttctt
     2341 taatgttagc aagcgtctca gcagcctgag cgaagtgcgg tttgtcctgt tcaggtgtca
     2401 tgtgtcggtc aagaatcgga caaagaatat cctgataaaa aacatagcgg ttgggattga
     2461 taccgctgag attgcctggt aggtctggta agaggttggc aaggtcaatc tgcataaaat
     2521 cctcaaccgt tagaccagta ttggtcttga agtgcgcaga caaactatct aggtcattgc
     2581 gatagctgag ttctgcccag atttgcaagc ttggtaggat agagaactgg gcagtttcac
     2641 caccattgtc tccccaaccc gttacgatga cttctttaat ctgattggca cggcaggctt
     2701 tattagcctc gatagccact agacggctaa aatgattgtg aggtgtaaag ccaatccact
     2761 tccaagctcc ccctgcaaat gcaaggtcat ggctaatctt gtgatgattg cggaaattac
     2821 ggttgtattt ttcctcgcta tcctgataat aatcccagta aaccagagtc acacggtctt
     2881 tgagacggtc taggtagaca cgagtttcct ctggaatttc cacatcacgg tcgtactggc
     2941 catccgctga catgagtttg aagaacatat cactccacat ctggcagtgg aaaccatatt
     3001 tgtcagcaat atccagcacg cgctccaagt gttggcacat gaggagacta cgatccacaa
     3061 caccgttcaa aatcaggtag cgtcccaaac caaccaagtg ggcttcgtcc atcccgatat
     3121 tgaccttgcg agtcttcagt ttagacaacg tggcaaacat gccatcaatc aggtcataaa
     3181 ccttttcttc gccaatgaga agaatgtcct ctacatcacg gagctcctgc acttccttga
     3241 caccccattt gacaaaggcc gacaagtggg ccaaggtctg gatgcatggt acaaaggtca
     3301 tgtcaaactg ttgggcatag gcttcgattt cctgcaactc ctcagctgaa taagctccac
     3361 ggaaatagcc aaagtaaggc tgcccttcaa tctggtaagt gtcttccatg tagagctcaa
     3421 aggttgagta gcccatgaga gccaatatct caatcatctg cttggcagaa gcgacattca
     3481 gcaccgcatt tcgagaacag tcaaccatgt aagccaaatc ttcgtaagct gcttgttcct
     3541 caatctctac tttatcagct tctgctagaa ctgttaccaa caaggataag gcacgataaa
     3601 gttggtgagg tttgcggtag gtcaattgat agtgaccttc ctcaccctcg atagagatag
     3661 aggcttggtc agactgagtg acagccactt ccacatctgg tagagaaata tgacctttta
     3721 aaacctcaat agcttgcgtt tgtttgggac taagtcctgt aaatcttacc attgcttttc
     3781 ctcctgtagc cagttgacaa gagcaccgta gagattggca tctgcgtgat aggtgcaggc
     3841 ctggataact ggtgcgaccg tgtattcttc gtaggtatcg acaaagttat caacagcctt
     3901 cttgacatct tggataaaat ctggattttg actgatagag ccacccagac tgatgacatc
     3961 tggatcgatg agatactgga tattgagcaa gccttgcgcc agattgcggt tcatgcgctc
     4021 aatggcttct tgacaaagag cattaccagc tgcggcctct tggtaaatct tgcgaccgtc
     4081 ccaatcagta tgaccagatt tttcaatcac gtatcgtacc atattcccag ttgatgctag
     4141 ttgcgaccag ttattaagtt tttcagcagg ggcaagggtt gtcatgtagc caaattctcc
     4201 acccagaccg tggcgacctc gatgaagtct accattgata atcatggctc cgccaatccc
     4261 tgtcccaatc acgacacagg ctgcattttc aagctctgga tgagctagta gttcactgag
     4321 tccaacgcag ttggcatcat tttctaaatg gacaggtagc tgatgagagc taagcgcctc
     4381 ataccaagaa aagccatgga tgtagggcac cgcactgaag ccatcaatca cacctgtctc
     4441 ttgatttacc gcacctggaa cgctcatagc aatcccactg taatcctgtt ctgacaagcg
     4501 ttgatctagc cacgctagta aatcctccaa gttttcaggc gttgaaatac ttgtcttatc
     4561 cagtattttc ccatcaggag tcagactggc aaacttaatc ccagtccctc cgatatcaat
     4621 cgttgcaatg gtcattgttt tcaccataaa aaggggcgaa tcagcagtta gtccttactt
     4681 tttcgcccct cctttctttt gatgtttact ttttgaaatt aaatttcttc ttttttgatg
     4741 aattctgtac gaatctcttg tggatccaat agtcctgaat gaactggata tggtcgctca
     4801 agtaagtcaa gaatggtttg ttgatgttct gatatgcgca cattttcttg actcatatta
     4861 tagtaacgga ggacatagcc ttcttcattt tccgccactt taaaggctgt tggacaaact
     4921 tgcggtatgc tgagaacaga atggctcaag aggctaccag tcgcaaccac gcttccttcc
     4981 tgtctagcaa gctgaaggct ggtaaacggt gtctgcaagg ctttggcacg acgataggct
     5041 gagaagcgtt cttgggcttg gtggcattca agtgcaaact cgacttcaaa ctcccgcaag
     5101 cattgtgctt ctggcgttgg gaagtagccc cagtcaccta gctcacctga tgcacgcaaa
     5161 atggtcacgg caatggtgtt atccccaagg atttcgtatt cattcaatcc cttattggat
     5221 acagtcaccc ctttttcatc gtcatacaga ctgacaaaag cttgttggtg ttgaggattt
     5281 tcagggtttt cccatgaagc agctggtttg tttggtcgtg tcaccacctc atagatactt
     5341 tcagaatcat tgcttggacg cgtgttatga gtcttgacca agagacggat acggtgatcc
     5401 ttggcagtgt tagtaaagcg agtcttgaag cggatttgtg gattgtcaac gaagacagtc
     5461 aactcagttt ccagaggaat gtttgtcaat tcttctgacc gtccagcctc acgcttcata
     5521 aactcgatga taccttgttg ctcttcttct agcttttcat ccgcactgac aggcacggtc
     5581 aattcatgtt tgagcaagat tttagcatag caagctgtgt tttccaagac ctcgtgaccc
     5641 ttaagctctg caaagattgg ctctgttcct tttggttgga aatagatata ctcgtttccg
     5701 atgtcacccc ggtcttcaaa gcggataaag tcttcatagg cttcgtgagt tgtcttgtca
     5761 tagactgtga tgttgtcatc cacactcacc gttacgaatg gtgtatcaat cactccgttt
     5821 tggtaaatac cctcacggtg ttcttgtttt ccttccagca attggaaggt tgtccaagaa
     5881 agcggcgcta ggtgaactgg aatggtcacg cgcacttgtc gagcaatacg agcttggcgg
     5941 aacttatctt ttggtaaatc gtactcaaaa ttagctccga ggtcttcgat tgtagcctct
     6001 acaggacgac catccaagtc ctccacacgg taacttggca agataagagc agccatcttt
     6061 ttgtagcctt ctgttgggtg caattccttg aaatcacaag tcgccacatc aatcactgtg
     6121 ctgacagtat cgaccttatc atgcaagcct gtgttaatga cagtaaagag atagtcactt
     6181 tgagccttat ccgtagcaat tttacccttc cactcgttga gcaagttact tttaacaaag
     6241 tttcctactt ggttgacctt ggcaaaacgc gtttccatct cgcggtgaac ttcgtccacg
     6301 ctacagccac agatactatc atgtggcgcg ttctgcaaaa gtgttttcca agcataggtc
     6361 aactggtcct tgtggttgtg gcccccagtg ataatcgtca agggttctac aacttgctct
     6421 agaagattgc tattttcttg gaaggcttgt ttgaggtaaa tgcgagatga agaagtgttg
     6481 gcaagtgtgt accagccgtc tgtttcctga ctggtcaact cacctgtaac cgttgataat
     6541 tgctctggta gggcactttc cacggcttgg acatattcat caaaagaact atgaacaaag
     6601 attacatccg ggaagagttc atttgccaca cgaatggctt cgctcagatt tttctggaca
     6661 ggctggtggt cacagccgtt catcatcaac cattggttgg tcgaagcgta ggcacgcaca
     6721 tctgacaatt tttgtttcca gaaggtcaag gcctcatctt tgtcaactgg gatttcattc
     6781 ccattactgt accagttggc aaagagaata cctaaaacac gactaccatc cacaccctgc
     6841 cagtacattt ctgaaaactg ggatgtaaac tgctcatctt caaggacttg gttgtcaaat
     6901 ccaatcggct tcacaccacg accaaaggcc gccacgtgaa tgcctgattt ttgaagaatt
     6961 tgaggcgctt gtcccatatt tccaaaggta tctggaaagt agccaatctg ggttgattta
     7021 ccccatttgg cagcttcttg ttgaccaatc aaggtattgc ggacattggc ttcactggag
     7081 atcaagtagt catcctgcaa gatgtaaaag ggaccaattt taagtttgcc ctcgtcaatg
     7141 tagcgttgga ccttgtcacg attttcaggg cgaatttgta agtagtcgtc aaggacaata
     7201 gtttgtccat ccaagtggaa actcttgaac tcagggtcat tttcaaagag atcaaagaga
     7261 ttgtcaaaca attccaccaa ctgcatacga tggctttcaa aaggcaagta ccactcacgg
     7321 tcccagtgac tatgtgagat aatatgtaca acaacatttt ccatgaagta aaacctcatt
     7381 ctaaatttaa attttaacgt tttaaatgta aacttgtgat tctgataaga atcggttgag
     7441 ctaaagcgag ccccttagcg aatatccaag taatccaaga ccaactcaca gaacatcatg
     7501 ttagcccagg agaaccattc acgagagtag agggtcggat catctacatg aaagctttcg
     7561 tgcatgacac ctgtaccacc atcgcaggca accagctgat ccagcaagaa ttttttctct
     7621 gccttatctc ttgttgtcaa tccttggata gaaagggcga ttggccagat atagcgatag
     7681 aaggtatgag aacttccgag accgctagcg tattctcctt ggtagaaata tggattttca
     7741 gagctcaaaa tggtacgacg agtagcttga tacacttcat catcgaccga acagtagccc
     7801 agatagggcg cagctagtag acttggtaca tttggatcat ccatgatgct ggcatttcct
     7861 aggccatcca cttcaaaagc gtaaatcttt tcgcccttgc tgttggtggt gtaagcgtag
     7921 tttttgattc cttcttggat ttcatcctga agacgcttgg catcagcaat aacactctgg
     7981 ctatcagcta ggtttaatgc tgcgaagatt tcttgcacat aacccaagac tactacagca
     8041 aacatatttg acggaatcaa gtaactatac tgacagcagt catcgctcgg acgaaaggct
     8101 gaccaagtca ttcctgtcac tgcaaaatca ggtccaaagc catcatttac caaggtgtct
     8161 tccttgcggt ccgtatctcg tacaaaacga taaggagagt tcttatgatc ttgttctacc
     8221 gtccacagat gaagaatttc cttagtcgct gcgacaaaag tctcatcaaa ctgactagtc
     8281 tcgccagttt ctttccagag gagataagac aactgcaaag gatagcaaag tgaatccacc
     8341 tcatacttgc gctcccagat ccagccgtta aggtctgtgt ggtcagtctc gtggtgccct
     8401 ttccagttct tctcaatgtt gaaggagtta gcatagggat ccttgagtac caaggtcatc
     8461 tgacgtttga ccaaacctgc aatggtctga cgcaggaggg catctctttt agctacatga
     8521 aggtagggtc tgagttgggc tgtcgaatct cgaagccaca tggcaggaat atccccagtc
     8581 aagacaaaag ttgaaccatc ttctaagatt tcaaccgtat tgtccaaggt atcggtgtag
     8641 caacgctcga aaacatccac ccactctgga tggtccttag cccgctctgc tacttcatct
     8701 agccattctc taacaatttc tttcgaataa atcatcgtgc aattccctct ttcaaacaag
     8761 tttcatgttc agtatatagc ttttgagaca gaaaatacat acacaaatga tagtcatttt
     8821 tctacaaaat agttattttt gaaactcctt ttctaaaggc cctctttttc tgatataatg
     8881 tagaaaaaca gctaaaggaa agactatgaa accactactt gaaaccatcg atacccgctt
     8941 tggaactacc aacaagcatg ccttttctcg gggaaatacc ctgccataca caggcgtgcc
     9001 ttttgggatg aattactttg tgccccagac cagtgaccag gatggatctt ggttttttga
     9061 tccgcatctg cctatctttc aggggattcg attaactcac cagcccagtc cttggattgg
     9121 ggactactct tggctccttc tgacacctgt caccagtcaa ctgggtggag acagcctctt
     9181 tcaccgccag tcttcttatg atatagataa agcttgcttc cagcctcact atttgaagct
     9241 cttttctctg cgttatcaga ttgaaaccca gctcacaccg acttgctacg gtgcttctat
     9301 tcgtttgaag caaaagcaag gcaaagccct ctccctctat cttcacgcaa cagatgaact
     9361 gacagtagag caagtagata agcgaactct tgccctgcga caagaaggta aaactgaaac
     9421 caacaaaaat tcgctaacga tgtttactgc cctgcaaatg aacacggata ttcttgctat
     9481 cagccaagaa gctggggact ggcgaattga cttagcaagt agtcaaaccg agatgcaact
     9541 agccacttct ttcatctctc cttctcaagc tctaatcaat ctacctcaag aagattttga
     9601 tagctgtaaa gcaagtgccc aagcggattg ggaaaatctc ctccatcgtt ttgacattat
     9661 agagacagga gaggctgacc gaaccttctt tgaccactgc ctctacagac tcttcctctt
     9721 cccacagact ttttatgagg ttgatgagtc agggcaagcc atccacatgg atatggctac
     9781 tggtactgtc aagcctggtg tcctctttag caacaatggt ttctgggata ccttccgcac
     9841 caccttcccc ctctttgccc ttatcatacc ggagcactat caacgctttt tagaaggttt
     9901 cctcaatagc taccgcgata ctggtttcct tccaaaatgg ctggctccag atgaacgggg
     9961 tatgatgcca ggtacacttt tagacggtat tatcgcagat agcgcctgca aggacatggc
    10021 ccctgaccta gaaggagaac tcttccaagc catgctcgaa acagccagca aagccgaccc
    10081 tctcggcatc aatggccgcc acggactagc ccaataccaa gaactaggtt acctctctac
    10141 cgaccaccat gaaagtgtta gccatactct agactatgcc tatagtgact tttgtatcgc
    10201 cagctgtgcc aaaaaactag agaacataga aatcgctgaa acctacaagg ctgcttcaca
    10261 aaattaccgc cagctatttg acgctgagac aggttacatg cgagcacgag acaatcaagg
    10321 aaactttcac cctgacttct ctccttatag ttgggggcga gactacgctg aatgctctgc
    10381 cattcaagct actttaggcg ttctccacga catccctggc ttaatccaac tgatgggtgg
    10441 aaaagaaacc tttagcaact atcttttgaa agcctgtcaa gatgctcccc tctttgaaac
    10501 aacaggctat ggttacgaaa ttcacgaaat gagcgagatg gctactgctc cttttggaca
    10561 gatagccatt tccaaccagc ctagtttcca cattccttat ctcttccgct acagcgatta
    10621 ccctgactac actgcccttc ttatcaagac gctccgtcag aaagcttttc acccaagttg
    10681 ggaagcctat cctggagatg aagacaatgg tagtctctct gcttggtaca tctggtcagc
    10741 tctcggattt tatccaacct gtccaggaaa accaagctat gacctcggaa tccctctctt
    10801 tgaccatcta cgagtctacc tcgctaaaga agataaatgg ctggatatcc atactaaaca
    10861 aaaccacaac cattttaact ttgtcaaaga atgccgactg gacaaaacac tcgtatcaac
    10921 tattcaacac caagacctct taaaagctga acaactaact ttcaccctca gctggttacc
    10981 aagtcactaa taaccaaaag aacctttgca tctcgcaaag gttcttcttt tatgaaactt
    11041 ggacttgaag ttgatccaca agctgaccat ccaccgttag gttcagatag aaatccaagg
    11101 ttttatctcc tgcgaaatac aaggttggta gcgtcagcct tttttcagtc tcaccagctt
    11161 gaaacgcaag gacataaatc tcgtcctgat aggcgacacc atggacacct gtccccggtt
    11221 gaatcgaaat cttagcctct aaagaggagc tactttcact acgttcaatc ctaaaatgaa
    11281 gagtctctcc ctttgccaca gctaggcttt tttctgcaaa tgctagttcc tgaacaactt
    11341 ctttttttga taacccagga gttttataaa gggaaatctt ggtcaataca gggatagatt
    11401 gtgcctcggt aatcatcacg cgcacctttt gtgcctctac tagcgaacct cgcaagaggc
    11461 gcttatgacc aactgtgaag cccctgccaa attcctgcca gacaccatcc acctctactt
    11521 gcacatggaa agcagaaata cgttggccta acttcaaatc ttctctcaac tcaattacat
    11581 caaaagtttt aggagaacct aagtcgagtt ctaactggat gggcaagtct gcatcgtttg
    11641 cccaagagct ggtctcaagg ccgtctgtca aatggcgaca agcaaagtct gcggaaagag
    11701 ctggaccaga tacctcagct cccagagcca aatcttcttt atagagctca ttgcgatagg
    11761 tcgcaaattc ataaagtcgt tcaatatcct ttgcatcaaa gagcccagct tgattcggcg
    11821 gaatattaag caagagtgga gttcctcgcc ctactgagtg aaagtagatt tcgaccaact
    11881 cctcgagaga cttaggatcc tgatcctcat ggtagaacca gcctggacgg atggaaacat
    11941 ctgcctctcc gattgaaaaa atcgtgcccg agggatcccc gtgctgaaga tagttcagct
    12001 ctgcttctgt tcctagttta tcaggattca ccttttgcca cagtggatca cctgcatacc
    12061 ctcgttcatt gccaatccag cggatactgg tgccttctgt tgaaaaaatc aagcaatcgc
    12121 cctgcaggtc acgaatggtt tcaaaccatt tttcaaattc ataattaacc ttttgcgcgc
    12181 cctctcctct ggcaccatcc atccaaacct cagcgaactt accagcattc ccatagttag
    12241 gatttgataa gatttccttc aactgagcca gataataggc attgtagtcc gcttctcggt
    12301 ccacatgata gaggggacta tgggcatccc acggtgacag gtagaccccc atatccatat
    12361 caaactctgt ggcagcttgg gatacttcaa ggagcaagtc gccctttcct ctcctccaag
    12421 gactgacctt aaccgaataa tctgtgtgag ctgtcggata aaggacaaag ccatcgtggt
    12481 gcttgaccac caaaatcaac tttttgaagc ccgtttcctt gagcacacga acccactcac
    12541 gcgcatccaa ctgactcggg ttaaagcgct caggatcctc ctgtccagtc ccccattctt
    12601 ggtcataaaa ggtattagga ccgaagtgga taaaagctgc tagttcatct cccagataag
    12661 ctagctgagt ctgacttggt aacggtccat gcggtttgat tttcttcatc gttatatatc
    12721 tccttcttta gcaacttctc ttgttcacaa ggttatccac aggttgtgga taataaacca
    12781 tgatctaaca gccagctctt tccaactcca actggtaggc tcaggatttt gctcctcaat
    12841 attccctctt caaacagctc tgtcatatgg ttctgctaac acctctcaga atgctactct
    12901 ccataattat tctcaggctt ctttcaggtc attgtgtgaa gaccattcac tctccagact
    12961 tttccacaga ttgtgaataa gtgattattc taaactatca cctcgtcgac tataacaaaa
    13021 aataaaaaga gggtcaataa gataattatc tcttcctaaa tactcagaaa agattctata
    13081 atttgtacaa ttgaacaaga aatgcttctc caaaatctat cttcaaaaga atgttgcatc
    13141 tttttatcct ttcacacaaa catttctctt tatacagatc caagtgataa ccagatgata
    13201 at
//
LOCUS       DAVLSL010000043        13031 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLSL010000043 DAVLSL010000000
VERSION     DAVLSL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.044"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..461)
                     /locus_tag="VXN30_000991"
     CDS             complement(<1..461)
                     /locus_tag="VXN30_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1357635.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENA"
     gene            complement(461..1540)
                     /gene="prfA"
                     /locus_tag="VXN30_000992"
     CDS             complement(461..1540)
                     /gene="prfA"
                     /locus_tag="VXN30_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1357636.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(1537..2127)
                     /locus_tag="VXN30_000993"
     CDS             complement(1537..2127)
                     /locus_tag="VXN30_000993"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1357637.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            2250..2432
                     /locus_tag="VXN30_000994"
     CDS             2250..2432
                     /locus_tag="VXN30_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1357638.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            2568..3941
                     /gene="mnmE"
                     /locus_tag="VXN30_000995"
     CDS             2568..3941
                     /gene="mnmE"
                     /locus_tag="VXN30_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1357639.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(3988..4923)
                     /gene="dapA"
                     /locus_tag="VXN30_000996"
     CDS             complement(3988..4923)
                     /gene="dapA"
                     /locus_tag="VXN30_000996"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1357640.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(4979..6055)
                     /locus_tag="VXN30_000997"
     CDS             complement(4979..6055)
                     /locus_tag="VXN30_000997"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1357641.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(6641..6713)
                     /locus_tag="VXN30_000998"
     tRNA            complement(6641..6713)
                     /locus_tag="VXN30_000998"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6678..6680),aa:Thr,seq:ggt)
     gene            complement(6813..7322)
                     /locus_tag="VXN30_000999"
     CDS             complement(6813..7322)
                     /locus_tag="VXN30_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1357642.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(7320..7416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            7445..7876
                     /locus_tag="VXN30_001000"
     CDS             7445..7876
                     /locus_tag="VXN30_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1357643.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            7958..8335
                     /gene="mscL"
                     /locus_tag="VXN30_001001"
     CDS             7958..8335
                     /gene="mscL"
                     /locus_tag="VXN30_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1357644.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(8377..9471)
                     /gene="hemH"
                     /locus_tag="VXN30_001002"
     CDS             complement(8377..9471)
                     /gene="hemH"
                     /locus_tag="VXN30_001002"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1357645.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            9626..10849
                     /gene="pepT"
                     /locus_tag="VXN30_001003"
     CDS             9626..10849
                     /gene="pepT"
                     /locus_tag="VXN30_001003"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1357646.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(10885..11514)
                     /locus_tag="VXN30_001004"
     CDS             complement(10885..11514)
                     /locus_tag="VXN30_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357647.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            11479..11700
                     /locus_tag="VXN30_001005"
     CDS             11479..11700
                     /locus_tag="VXN30_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357648.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(11809..12285)
                     /locus_tag="VXN30_001006"
     CDS             complement(11809..12285)
                     /locus_tag="VXN30_001006"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1357649.1"
                     /translation="MTPNNGVSAADDGYVFNPNDIVRDTGDAYIVRHGDHYHYIPKSS
                     LNNPPSHSNTEEAGSSSSSVLSNPSLHVHHEEEDGHGFDANRIISEDSEGFVIPHGDH
                     NHYIKVQTKGYEAALKNKIPSLQSNYQPGTFDEKAVLAKVDQLLADSRSIYKDRLS"
     gene            complement(12325..12465)
                     /locus_tag="VXN30_001007"
     CDS             complement(12325..12465)
                     /locus_tag="VXN30_001007"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1357650.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAA
                     KV"
     gene            complement(12509..13031)
                     /locus_tag="VXN30_001008"
                     /pseudo
     CDS             complement(12509..13031)
                     /locus_tag="VXN30_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         3799 a         2767 c         2457 g         4008 t
ORIGIN      
        1 gcattctctg atgcaagctc taaggcctct tgagaaacat ctgctgctgt cactgaccaa
       61 tctggtctat tttttgctag agcgagagca atagctccac tacctgttcc aatatccaga
      121 actgagagat tcgtctcaag attttcagtt agaataagct ccactaactc ctctgtttct
      181 ggacgaggaa tcaaaacccg ttcatccacc tttaaatgca ttccataaaa atctgcctgt
      241 ccaatgatgt actgagctgg cttgtgagct gctagttgct ggtaaatatc ttctacaaat
      301 tgtttttctt cctctgttgt cacctcctgc tggagggcaa aaataaagtc tgtaaaagat
      361 agatttttca gactacgata gacaaaagag aggctttccg cttcctctcc ttgtcttatc
      421 aactcttctt caaaatttga aaataattga gctaatttca ttatttgttt aattcttcta
      481 gtttttgtgt ttggtcataa agcaccaagg catccacaac ttcgtccaat ttaccagaca
      541 aaatcgtatc tagtttttgg agggtcaagc cgatacggtg gtctgtgaca cggttttgtg
      601 ggaagttata agttcggatc cgttctgaac ggtcaccagt accgattgtc gacttacgct
      661 cagcgtcttg ttcatcctga gcaatctgag caaagtggtc agcgacgcgt gcacggataa
      721 tcttcatagc cttctcgcgg ttcttctgct gggtacgttc ttcctgcatc tcaaccttga
      781 tattggttgg caagtgaacg atacgaacgg cagtcgcaac cttattgacg ttctgtccac
      841 cagcaccaga ggcgtgatag atgtcgacac gaaggtcttt tggatcaatg tcgtattcaa
      901 cctcttcaac ttctggcata acaagaactg tcgctgtcga agtatgaaca cggccttggc
      961 tttctgtcac aggaacacgt tgcacacggt gggcacctga ttcatactta agcttagagt
     1021 atacagactg acctgaaacc atagcaacca cttctttaaa accaccgaca ccattcatag
     1081 aggcttccat gacttcaaag cgccaacctt gggcttccgc atacttttgg tacatagtta
     1141 gcaaatctcc agcgaaaagt gccgcttcgt ctccaccagc tgctccacgg atttcaagga
     1201 tgatattctt gtcatcgttt ggatcctttg gaaggagcaa aattttcagt ttttcttcat
     1261 attcttcttt ttcagccttg gcatctttga gttcttgctt ggccatttct tccaagtccg
     1321 catctccgcc tgattcctta atcatctctt cggcatcgac gatattttga aggacttgtt
     1381 tatactcacg gtaggctatt acggtgtcac gattggaagc ttcttctttt gaaagctcca
     1441 taaaacgctt ggtgtctgaa acgacatcag ggtcactcag caattctcct agttcttcat
     1501 aacggtcttc tacagcttgt agttgatcat agatgttcat ttttcttcat tctccttatt
     1561 gatttcaggg gcaaaataat gtttacggca aaccgagata taggtttcat taccaccaat
     1621 ctggatctgt tctccatcgt aaacgggcag tccatcctgt gttcgcaaca ccatggtcgc
     1681 ctttttcttg caatactgac agatggtctt gatttcgtca atcttgtctg ctaaaagcaa
     1741 gagatatttg gaaccttcga acaattcatt gcgaaagtca tttttcaagc caaaagccat
     1801 gacgggtatg tctaactcgt ccacaacacg agctaggtca taaacatggt ggcgtttgag
     1861 aaactgggct tcatcgacca aaacacagta aggtttttct ggtaggtctc ggatatagcc
     1921 aaagatatcc gttgtttcct caatcgcaag ggcagggcgt ttcatgccaa ttcgactcga
     1981 cacatagcca acaccgtcac gcgtatccag agccgaggtc ataatcacaa caccttttcc
     2041 ttgctcctcg tagttatagg ccactttgag aatctcaatc gttttaccag agttcatggt
     2101 cccataacga tagtacaact gtgccatgtt tcttgcttca cgtccatttc taaatttttg
     2161 ctacattcta gtatatcata attttcttaa gctttaaacg gcaaaatgtg gtaaaatgga
     2221 agaaatcaaa actagtggag gaagctatta tgccatttgt acgcatcgat ttatttgaag
     2281 gacgcacgct cgagcaaaag aaagctcttg ctaaggaagt aacggaagcg gttgttcgca
     2341 acactggagc acctcaatct gctgttcatg tcatcatcaa cgacatgcca gaaggaactt
     2401 atttcccaca aggggaaatg cgcaccaaat aagctagctt aagcagaatt gcttaggctt
     2461 tttcaatctc caagtagcac ccattgaaga aatagtctaa atttgttaca atttgaaaag
     2521 agacttggaa aattccaaga aaagagcaat taattaaagg aaacattatg attacacgtg
     2581 aatttgatac catcgctgct atctctactc cactaggtga aggggctatt ggtattgtcc
     2641 gcctgagcgg aacagacagt tttgctattg tgcaaaagat ttttaaagga aaagacttga
     2701 acaaggttgc cagccacact ctcaactacg gtcacattat tgatcctctg actggtaaag
     2761 tcatggacga ggttatggtt ggggctatga agtctccaaa gaccttcact cgtgaggata
     2821 ttatcgagat taacacccac ggtgggattg cggtgaccaa tgaaattctc cagctagcta
     2881 ttcgtgaagg ggctcggttg gcagaacctg gtgaatttac caaacgtgct tttttaaacg
     2941 gtcgcgtaga cttgacacag gcagaggctg tgatggatat catccgtgcc aagactgaca
     3001 aggccatgaa cattgcggtc aaacaattag acggctccct ttctgacctc attaacaata
     3061 cccgtcaaga aatcctcaat acacttgccc aagttgaggt caatatcgac tatcctgaat
     3121 atgatgatgt tgaggaagct actactgccg ttgtccgtga gaagactatg gagtttgagc
     3181 aattgctaac caatcttctt agaacagccc gtcgcggcaa aatccttcgt gaaggaattt
     3241 caacagctat catcggacgt cccaacgttg ggaaatccag ccttctcaac aacctcttgc
     3301 gtgaggacaa ggctatcgta acagatatcg ctgggacaac acgagatgtc atcgaagagt
     3361 acgtcaacat caatggtgta cctctcaaat tgattgatac agccggtatt cgtgaaacag
     3421 atgacatagt ggaacaaatc ggtgttgagc gttcgaaaaa agccctcaag gaagccgact
     3481 tggttctact agtgctaaat gccagtgaac aactgactgc gcaagacaga caacttcttg
     3541 aaattagcca agataccaat cgcattattc tacttaataa aaccgacctg ccagaaacga
     3601 ttgaaacttc gaaactacct gaagacgtta tccgtatttc agtccttaaa aaccaaaaca
     3661 tcgacaagat tgaagagcga atcaacaacc tcttctttga aaatgctggc ttggtcgagc
     3721 aagatgctac ttacttgtca aacgcccgtc acatttccct gattgaaaaa gcagttgaaa
     3781 gcctacaagc cgttaatcaa ggtcttgagc tggggatgcc agttgatttg cttcaagttg
     3841 acttgactcg tacttgggaa atcctcggag aaatcactgg ggatgctgct ccagatgaac
     3901 tcatcaccca actctttagc caattctgtt taggaaaata agaaaaatcc atgatccttc
     3961 attcggtcat ggattttatt gtctttatta gtaatctggt cttaagaccc ctgttacagt
     4021 tgccttagtt gcttcgtagt cgccatctac gacaaccttg ataatgcgtt tggcatcttc
     4081 ttctggtgct ggaacaagag gtagacgagt gggtccagct tcaaatccca tatagttaag
     4141 aattgcctta actggagcag gacttggata agagaagaga gcattaacct taggaatgaa
     4201 tttacgctga attgctgcgg ctttcttcat atcgctttct gcaatggcag taaacatctc
     4261 gtgcatttca tccccatttg tatgagaggc aacagaaata accccatccg ccccaaggtt
     4321 catggcatgg aaagcatctc catcctcacc tgtataaatc aagaactctt caggcttgtg
     4381 ctcaatcaag taagccatat tagccaagct agtacattct ttgacaccga taatatttgg
     4441 atggtcagcc aagcgaagca tggtttctgg agtcaattcg accactacac gccctggaat
     4501 gttatagata ataattggta ggtcagaagc atctgcaata gccttaaagt gctgatacat
     4561 cccttcttga gaaggtttgt tgtagtaagg aacaatagca agcccagctg cgaaaccacc
     4621 aaattccgct acttctttga caaactcaat agagtcacgc gtatcattag tacctacacc
     4681 cgcaatcaaa ggaacgcgtc cattgacaac cttttgtaca gccgcaaaca actccaactc
     4741 ctcatcgtgg gtcaaagttg gactctcagc agtcgttcct gcgagaagaa ttccatccgt
     4801 atgatgggcc aataaatgct caatcaaggc tggaatagca tcaaagttaa tggaaccatc
     4861 ctcatggaag ggggtaataa aggctgtaat gattttacat tcttttaaat cttgataaga
     4921 cacgaaaaca cctctctatt tcaaagaagg agttcatctg aactcctaaa cgatatgact
     4981 attttaattc aaatttcaat tcagctgttg gacgaaccaa tccacgttca tgaagagttt
     5041 cagcaatctg aactgagttc caagcagcac ctttgagaag gttatctgaa acaacccaca
     5101 tgtgaattcc tttttctgca tccaagtctt tacggatacg accaacaaag gtatcacgtg
     5161 aaccaactgc attgatggct tgaggataga tttgatgaac tacatcatct tcaagaacag
     5221 cacctgggaa ggatgcgata gctgctttga cttcttcaat tggagccact tcctttgttt
     5281 caatataaac tgactcagag tgagctgaca agactggaat acgcacacat gtcgcagata
     5341 ctgcaatgct atcatcttcc ataattttct tagtttcctt ggtcatcttc atctcttcgt
     5401 acgtgtaatc attatcagtg aagacatcaa tttgtggaag agcgttaaag gcgataggat
     5461 aatgtttctt gtcaccacct gaaggcaaga tttccgcatg caaatcacgt ggtttcactc
     5521 catcattcaa gacctcacga agttcacgtt gtgtctcaag aattgctccc ataccagcac
     5581 ctgaaacggc ttgataagtt gaaacaatga tacggtccaa gccccatttt tggcgaaccg
     5641 gctcaagagc caccatcatt tggattgttg aacagttagg gcaggcaatg attccgttgt
     5701 gggcatcaag tgcatgagca ttgacctctg gaacaaccaa aggaacatct ggattttgac
     5761 ggaaataaga tgtattatcc actactacca cgccagcttt tactgcgtat ggtgcatact
     5821 tagctgatgt cgaaccacct gctgaaaaga gagcaatatc aactccttca aaagctgttt
     5881 cagtcgtttc ttcaatcgta atatcttgat ctttaaattt caatgactta cctgctgaac
     5941 gtgcagaagc aaggtaacgg attttatcga ttggaagtgt tgattcttcc aacattttta
     6001 tcatctgagc accgacagca cctgtcgcgc cgactacagc aactgtatat cccataaata
     6061 acctctttcg gaattttcta aaaatttcta taatagaagt attatactac tttttccatg
     6121 aattgcaaag ctttttcatc attttttgga aaataaaaat ccccaatcgc tagactaggg
     6181 attaagaggc atcttcatgt gatgagcagg ttcacacaac tcatcaaggt ccgctcctgc
     6241 gttatgacct ccttatgctc aatagtagcc cgaagactac ctatcgactc atcgcatcta
     6301 ctattctact aaagagaatg acttttgtca acagacgaaa ctctttatac tcttcgaaaa
     6361 tctcttcaaa cttcgtcagc gtcgccttgc cgtatgtatg gttactgact tcgtcagttt
     6421 catctacaac ctcaaaaaca tgttttaagc tgacttcgtc agttctatct acaacctcaa
     6481 agcagtgctt tgagcaacct gcggctagtt tcctagttta ctctttgatt ttcattgagt
     6541 attagtttta tttacacagg tgcataagaa atatattgga aaaattgggg gcagacataa
     6601 caaaaaccct tgtaaatcaa gggcttttct gtctatttaa tgctaattgc cgggattgaa
     6661 ccggcgacct catccttacc atggatgcgc tctgccaact gagctaaatc agcttactta
     6721 agaagtatac tatattcaga aaaacttgtc aagtttcctt agaaattttc cataggaaaa
     6781 agccaggtaa gaactaccta gcttatcttc ttctatttac ttaaatcaat tcgacgacca
     6841 attttaccaa taataatcgc tccaataatc aatgaagcaa attcaccgat tcctgttgta
     6901 aaccaagtaa ggaagaatgg taattctgct acaatattta actctgcagc gatagtaaac
     6961 atggaaattg aaaagaggat tgaaaagaag aaatgatctt ttcgaatcaa tccgttaaag
     7021 aggtaatcct tgctatattt tgcaaaaagc caaacaccta aactgaggaa tactaaggtt
     7081 gaaccaccac caacaaagac atctagcagt ccgaagctaa agaaattagc aatcatacaa
     7141 ccgatggtaa ctccgatgat gtacttagga ttgtaaaaag ccataaagtt catcatttct
     7201 gaaatacgaa actgataagc accatagctg atggcattta ggggcggtgt gacagtcaaa
     7261 acgacgtaga tagcagcgac gattgcaata tctgcaacat cacgaatagt taattttttc
     7321 atcttttctc ctttggcggt tatccgcgtg taatatgctt ggtgaaagaa gctaagcacc
     7381 aagggttgat tcattcaacc ttactagtat agcacaatag cctgctttat gctatactta
     7441 aagcatgaaa aaacctattc aaaaattttt taacaatgaa atcttagcct atctcttttt
     7501 tggccttgcg acaactctag tttcgattct ctcacgacta gtcatttatc aactaagtca
     7561 tcaggaactc cttgctacgg ccttggcaaa tattatcggt atcttatttg cctttatcac
     7621 aaatgatacg attgtattta agcaaaaaaa gaaaaataga cttatccgtt tagtgaaatt
     7681 ttctcttgct cgcctttcta cttttctgtt agacttgttt ttaacttttc tctttgtaac
     7741 ccaatttcct cacatcatag ggcaattcgt aactgaaaac attgataaga taaatgctat
     7801 tgaaacaata attgcacaaa tattcattat tatcatcaat tatatcttga gcaaggtcta
     7861 tatttttaga aaataatgct ccatttatga gccttttaat tgccttctat aatgatttag
     7921 gatacaatga aacacgaaac attgaaagga aaacagtatg ttaaaaaatc taaaatcgtt
     7981 cttgcttcga ggaaatgtta ttgaccttgc tgtcggtgtt gtcattgcct ctgcttttgg
     8041 tgctatcgtt acttcacttg taaacgacat tatcactcct cttattttaa atcccgcttt
     8101 gaaagctgct aaagtcgaac gtatcgctca actttcttgg catggagtcg gctatggtaa
     8161 ctttttaagt gctattatca attttatctt tgtgggtacc gccctcttct ttattatcaa
     8221 gggcattgaa aaagcacaga aactgactgg cataaagaaa gaaaaaactg ccgaaaaaaa
     8281 accaaccgaa ttggaagtcc ttcaagaaat aaaagctctc cttgagaaaa aataatcgat
     8341 taaaaggatc tagcgcaaag ctaaatcctt ttttctttac tctttgggta acttgcctgc
     8401 tttttcaagc atttttttga taagataagg catcttaaca ttctcccgcc cttttttctc
     8461 aatcagtttt ttcacaaatt ctggcatttg taaatcttca gattcagcta aaatgttttt
     8521 cgtctcgtct gaaggaacta actcatcaaa ggtctcttct tctgggagaa attccttaaa
     8581 ctcttgattt tcattgactc taactgtatt gaccaaagca tcaatcaaac gagaatctgt
     8641 atttggcatt ggtggacgat ggtagtttac cccaaattcc tggcataagt cataacattc
     8701 tacatcattg tcaaacaaga cctcaatatg ctcactgatg aaactaatag gtacaaaaat
     8761 ataatggtct ggatgttctg tctgttctct gagatactcc aaaacatctg gtttaatcca
     8821 tggaatacca atatcactct cactctgcca agtgttggta tattgttctg aactcaagcc
     8881 cagtttttca gcgactagct tgctattttc gaaaatttgg tctatataag gatcaccaaa
     8941 gtccaaggca aaaatgggca cactatgggc tgaaaagatg actttaaagc tgtcttgctt
     9001 tacttcttct tttaaaatct tagcaatttc atctgcccaa tagtttaaga gcgcttcttc
     9061 ctgataccag tccttaatga ccaaaaattg aatttgtttg ctttctaaaa atttttcata
     9121 gcccatgaca gagtagaaag aataatgggg ctccaaaatc aagcaaatac actgttcaat
     9181 tccgtctgct tccatttgac cgatcacatc tggaataaag ggactggaaa acttattagc
     9241 aaagtagaca ctgtattcat tccccagcct agcttctacc aaggcaacct cttgacgggt
     9301 aattttttgc agaggcgtgc ctccaattcg tacataatta tcatagagag tctgaatctc
     9361 gtggtcttgg ggtctcaccc cacgacgaat atttgtgaaa aaatcagcta caccttcaaa
     9421 ggtaatctct tctggtgaac cgaaggtcat cattaaaatt gcttttttca tgactgcttc
     9481 cttgtgatgt ttttatcaag tttattttat cattaattaa tcgataagta aacgctaata
     9541 tagggacatt ctcaatcttc tgtcttttgt ttttctcttc tttctatgat acaatggaaa
     9601 aaataaattc aaaaggagtt tttttatgac ttatccaaat ctcttggacc gcttcttaac
     9661 ctacgttaag gtcaacacgc gctctgatga acactctact actactccaa gtacacagag
     9721 tcaggttgac ttcgcaacaa atgtcctaat tcctgaaatg aaacgtgttg gactgcaaaa
     9781 tgtttactat ctaccgaatg gttttgctat tggaaccttg ccagccaacg atccgtcttt
     9841 aacacgtaag attggtttta tatcgcacat ggatactgct gattttaatg ctgaaggagt
     9901 caatccacag gtaattgaaa actacgatgg tggtgtgatt gaactaggga attctggttt
     9961 caaactcgat ccagctgact tcaagagtct tgaaaaatat ccaggacaaa cgctcatcac
    10021 aacagatgga acaagcttgc taggtgctga tgacaagtca ggaattgctg aaattatgac
    10081 agccattgaa tatctaactg ctcatcctga aattaagcac tgtgagattc gtgttggttt
    10141 tggtccagat gaagaaatcg gtgttggtgc caataaattt gatgcagaag attttgatgt
    10201 ggattttgcc tacactgttg atggtggtcc actaggtgaa cttcagtacg agactttctc
    10261 agccgctggt gctgaattgc atttccaagg ccgtaatgtc caccctggta ctgccaaaga
    10321 gcagatggtc aatgcccttc agctagcaat tgattttcat aatcaacttc cagaaaatga
    10381 ccgacctgag ttaactgaag gttaccaagg tttttaccat ctaatggatg tgacaggtag
    10441 tgttgaggag gtgcgtgcaa gctacatcat tcgtgatttt gaaaaagatg cctttgaagc
    10501 gcgtaaagca tccatgcaat ctatcgctga taagatgaat gcagaacttg ggagctaccg
    10561 tgtcactctc aacttgacag accagtacta caatatgaaa gaagtcattg aaaaagatat
    10621 gactccaatt accattgcta aagccgttat ggaagatcta ggtatcacgc ctattatcga
    10681 accaatccgg ggtggaacag acggctctaa gatttccttt atgggaatcc caactccgaa
    10741 tatctttgca ggtggcgaaa atatgcacgg acgttttgaa tacgttagcc ttcagactat
    10801 ggaacgtgca gttgatacca tcattggcat cgtagcttat aaaggctaaa aagacgaggt
    10861 agctcagcta cttcgccttt ctttttattc tactggtttt tcttgatttc cagtagttgt
    10921 agaagattct gttgtttcat tttctgaagt tgattcagca ggtttagaat ctcttgtatt
    10981 gcttggtttg ttttcgtcgc tagcaatttc aatgttagat tctgcagttg cgtttggttg
    11041 gttctcagca ctggtgttat cactatttgc ttcagcattt cttgctggac ttgtttcttc
    11101 acttgcgcta gcttttgact ggatttgatg attcaaaact agaatagctt ttgtcgattc
    11161 aagtaaagct gttttgtctt tactcttagc agaaagttga tctaataatg catccacctt
    11221 atcaaagtcc gcatcagatc cattattact ttctaaataa gagtgaagcg acatgagaat
    11281 atcgtagagt ttttgataga gtacaagtgt ctgagaatct tgctcagcat tttccttttc
    11341 ttgttgaagg gcgctagcga tacgagtcaa gacatctttt acctgactgt ttacttcatc
    11401 caagtctgca tcagccttgt ttgtggcagc ttttagattt tctacttctt ctgccaagga
    11461 ttgtctgatt ccttcttcat ggatttgttc caagagttga tttaccttgc tcaaaagact
    11521 ttctacttct tccttgctat ctgtcgcaga ttattggttg ccatctacca tgtactccta
    11581 aaacaggaga gttataatcc aagattacaa ggccttacag aaataagaaa tccagataag
    11641 acaatgttcg tccaagacgc tattcgcttc gcacagcagc acggattcaa tatgctttaa
    11701 ttttaaagtt taggtgtcaa gacctctttt tagtgtgccc aaaatttaga gaagtaatca
    11761 atcaactaac ttttattttt ttcaaacttt cagtaaactg acctaaagct aactcaatct
    11821 gtctttgtag atgcttctgc tatcagctag aagttgatct acttttgcca agactgcctt
    11881 ctcatcaaaa gttccaggtt gatagttgga ttgcagggat ggaatcttgt ttttcaaagc
    11941 cgcttcatat cccttagttt gaaccttgat gtagtgattg tggtcgccat gaggaatcac
    12001 aaaaccttct gaatcttcac ttataattcg attggcatca aaaccatgac catcttcttc
    12061 ctcatggtgg acatgtagtg acggattact taatacagaa ctagaagaac ttcctgcctc
    12121 ttccgtgtta gagtgtgatg ggggattgtt aagagatgac ttaggaatat aatgatagtg
    12181 atccccgtgt cttactatat aagcatcacc tgtatctctg acaatatcat tagggttaaa
    12241 gacataacca tcatctgctg cagaaacacc attattcggt gtcaccgaca aagattgact
    12301 gagagctgta gtattccctg ataattatac ttttgcagct gctaattcac ctgccgacaa
    12361 gtcactctca ggaatgaaat gatagtgacc accatgtggt actatagtag gttgaaatag
    12421 aatatgagca aattgataag gggattttaa agtaatttct aacaatgatt tagaaactat
    12481 gatgtgctat tctaaattca actcactata tataaccatc atcggtagta taacgtccct
    12541 gtaattttgc tacagatact tctgcactag ctcctttatc gtctttacca tgttcttgtt
    12601 tttggcgatt gatttcatct tttgttcgta cattttctgc atgagcttga tctttaaggt
    12661 aaacataata ctttccatct accttaataa tatatcctcc cttaacctaa ctgacgatat
    12721 cttgatcttt cggctgatag ttgggggctt tcattaatag ctcttcacta aagagcgcat
    12781 caaaaggaac tttaccatta tagtagcgat aatgatcgcc atgagaagtt acataacctt
    12841 gatctgtaat cttaataaca atttgttttg cttgaattcc ttctttttga ctaacctagt
    12901 ctggagtcaa attttcagtc ttcttagtgt ctttattact gtttacatat gaaacacgat
    12961 ttttatctgt attggcctgt tagctatgtt ggttcagagt ataaacacac agacttaagg
    13021 aaaggataac a
//
LOCUS       DAVLSL010000044        12980 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLSL010000044 DAVLSL010000000
VERSION     DAVLSL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.045"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..681
                     /locus_tag="VXN30_001009"
     CDS             <1..681
                     /locus_tag="VXN30_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1357651.1"
                     /translation="APKPETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNAN
                     GAMATGWAKVNGLWYYLNSNGAMATGWLQYNDSWYYLNTNGAMATGWLEYNGSWYYLN
                     ANGAMATGWLEYNGSWYYLNSNGAMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYY
                     LNANGSMATGWVKDGDTWYYLEASGAMKANQWFKVSDKWYYVNGLGALAVNTTVDGYT
                     VNENGEWV"
     gene            1081..2202
                     /gene="mnmA"
                     /locus_tag="VXN30_001010"
     CDS             1081..2202
                     /gene="mnmA"
                     /locus_tag="VXN30_001010"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1357652.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTTLLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            2343..2798
                     /locus_tag="VXN30_001011"
     CDS             2343..2798
                     /locus_tag="VXN30_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1357653.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYTIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGFSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKFEDIQLVPAFLKTALPDWEGQLRHIHREE"
     gene            2808..4721
                     /gene="mnmG"
                     /locus_tag="VXN30_001012"
     CDS             2808..4721
                     /gene="mnmG"
                     /locus_tag="VXN30_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1357654.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDKILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDAVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            4910..4999
                     /locus_tag="VXN30_001013"
                     /pseudo
     CDS             4910..4999
                     /locus_tag="VXN30_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5100..6779)
                     /locus_tag="VXN30_001014"
     CDS             complement(5100..6779)
                     /locus_tag="VXN30_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1357655.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQGEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6781..7014)
                     /locus_tag="VXN30_001015"
     CDS             complement(6781..7014)
                     /locus_tag="VXN30_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1357656.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            7396..7617
                     /locus_tag="VXN30_001016"
     CDS             7396..7617
                     /locus_tag="VXN30_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357657.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7830..7982)
                     /gene="cibB"
                     /locus_tag="VXN30_001017"
     CDS             complement(7830..7982)
                     /gene="cibB"
                     /locus_tag="VXN30_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1357658.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7984..8169)
                     /gene="cibA"
                     /locus_tag="VXN30_001018"
     CDS             complement(7984..8169)
                     /gene="cibA"
                     /locus_tag="VXN30_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1357659.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            8347..9030
                     /gene="tsaB"
                     /locus_tag="VXN30_001019"
     CDS             8347..9030
                     /gene="tsaB"
                     /locus_tag="VXN30_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1357660.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYVGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            9027..9464
                     /gene="rimI"
                     /locus_tag="VXN30_001020"
     CDS             9027..9464
                     /gene="rimI"
                     /locus_tag="VXN30_001020"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1357661.1"
                     /translation="MIEIKRIQKHSDLAQAIYAVMIAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            9454..10464
                     /gene="tsaD"
                     /locus_tag="VXN30_001021"
     CDS             9454..10464
                     /gene="tsaD"
                     /locus_tag="VXN30_001021"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1357662.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(10504..11810)
                     /locus_tag="VXN30_001022"
                     /pseudo
     CDS             complement(10504..11810)
                     /locus_tag="VXN30_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(12143..12437)
                     /locus_tag="VXN30_001023"
                     /pseudo
     CDS             complement(12143..12437)
                     /locus_tag="VXN30_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12544..12837)
                     /locus_tag="VXN30_001024"
                     /pseudo
     CDS             complement(12544..12837)
                     /locus_tag="VXN30_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3698 a         2583 c         3045 g         3654 t
ORIGIN      
        1 gctcctaaac cagaaactcc aaaaacaggc tggaaacaag aaaatggtat gtggtacttc
       61 tacaatacgg atggttcaat ggcaacaggc tggctacaaa ataatggctc atggtactac
      121 ctcaacgcta atggtgctat ggcaacaggt tgggctaaag tcaacggctt atggtattac
      181 ctcaacagca atggcgctat ggcgacagga tggctccaat acaatgattc atggtactac
      241 ctcaatacta atggtgctat ggcgacaggt tggctagaat acaatggttc atggtactac
      301 ctcaacgcta atggtgctat ggcgacaggt tggctagaat acaatggctc atggtactac
      361 ctcaacagca atggcgctat ggcgacaggt tggctccaat acaatggttc atggtactat
      421 ctaaacgcta acggcgctat ggcaacaggt tgggctaaag tcaacggttc atggtactac
      481 ctcaacgcta acggttcaat ggcaacaggt tgggtgaaag atggagatac ctggtactat
      541 cttgaagcat caggtgctat gaaagcaaac caatggttca aagtatcaga taaatggtac
      601 tatgtcaatg gcttaggtgc ccttgcagtc aacacaactg tagacggata tactgtcaat
      661 gaaaacggtg aatgggttta agccgattaa attaaatcat gttaagaaca tttgacattt
      721 taattttgaa acaaagataa ggttcgattg aatagattta tgttcgtatt ctttaggtac
      781 ctgtcttatg atttcaggaa atgtcattaa aaaaacgact ccttttctct aacttggaaa
      841 atagattaga gaaaatgggt tgttttattt attatagtta tttgaatgaa gataagaaga
      901 aggtatactc acatcattca caaatctgta tattgactat aagttttaaa aaacaatttt
      961 taagctcttc cttgtcttct ctaaccaagc gtgttataat gaatactgct caagcgacct
     1021 tcaatcgtga agcacacacg accttcaatc gtgaataaac gaatagatgg gagacttacc
     1081 atgagtgata actctaaaac acgtgttgtc gtggggatga gtggtggtgt tgattcgtcg
     1141 gtgacgactc ttcttctcaa ggagcagggc tacgatgtga tcggtatctt catgaagaac
     1201 tgggatgaca cagatgaaaa cggcgtctgt acggcgaccg aagattacaa ggatgtggtt
     1261 gcggtggcag accagattgg cattccctac tactctgtca attttgaaaa agagtactgg
     1321 gaccgcgttt ttgagtattt cctagcggaa taccgtgcgg gacgcacgcc aaatccggac
     1381 gttatgtgca acaaggaaat caagttcaag gcctttttgg actatgctat gaccttgggg
     1441 gcagactatg tagcgactgg gcattatgct cgagtggtgc gtgatgagga tggcactgtt
     1501 cacatgcttc gtggcgtgga caatggcaag gaccagacct atttcctcag ccaactttcg
     1561 caagaacaac ttcaaaaaac catgttccca ctaggacatt tgaaaaagcc tgaagttcga
     1621 aaactagcag aagaagcagg tctttcgact gctaagaaga aagactcgac agggatttgc
     1681 tttatcggag aaaagaactt taaaaacttc ctcagcaact acctgccagc tcagcctggt
     1741 cgcatgatga ctgtggatgg tcgcgatatg ggcgagcatg caggtcttat gtactatacg
     1801 attggtcagc gtggcgggct tggtatcggt ggacaacacg gtggtgacaa cgcgccgtgg
     1861 ttcgttgtcg gaaaagacct gagcaagaat attctctatg taggccaagg tttctaccat
     1921 gattcgctca tgtcaactag cctagaggct agccaagtcc actttactcg tgatatgccc
     1981 gaggagttta cgctagaatg tacggctaaa ttccgttacc gtcagcctga ctctaaggtg
     2041 accgtccatg tcaaaggaga caaggcagaa gttatctttg cggaaccaca acgcgcgatt
     2101 acaccaggac aggcagttgt cttttacgat ggcgaagagt gtctaggtgg cggtttgatt
     2161 gacaatgctt accgcgatgg acaagtttgt cagtacattt agattgacaa attttctcaa
     2221 tttgctacaa taataacagc aatagaaatg atggtcaaag ctcatggatg ttgcaggctt
     2281 ttttgtcctg cacttctttg gagttttgac tgtttttgtg tcgtttaagg gaaaggacaa
     2341 gaatgactca acaagacttt cggacaaaag tagacaatac ggtttttggt gttcgggcga
     2401 cagctttgat tcttcaaaat cgcaagcttc tagtcaccaa agacaagggc aagtattaca
     2461 ctatcggcgg tgcgattcaa gttaacgaaa aaacggaaga cgcggtagtc cgtgaagtga
     2521 aggaagaact gggtgtcaaa gctcaagctg ggcagctagc ttttgtggtt gaaaatcgtt
     2581 ttgaacagga cggtttttcc tatcacaata tcgaatttca ttatctggtg gacttgctgg
     2641 aggatgcccc attgaccatg caggaagatg agaaaaggca gccctgtgag tggattgact
     2701 tggataagtt tgaggatatc cagctagttc cagccttttt aaaaacggcc ctaccagatt
     2761 gggaaggcca actaagacac attcatcgtg aggaatagga gagaaacatg acttatcatt
     2821 ttactgaaga atacgatatt attgtaattg gtgcgggaca cgctggggtt gaggcttcct
     2881 tggccgctag ccgtatgggc tgtaaggttc tacttgcgac catcaatatt gaaatgctgg
     2941 ctttcatgcc ttgtaatccc tctatcggtg gttctgctaa ggggattgtc gtacgtgaag
     3001 tcgatgccct cggtggcgag atggccaaga ccattgacaa gacttacatc cagatgaaga
     3061 tgctcaacac agggaagggt cctgctgttc gtgcccttcg tgcgcaggct gacaaggaac
     3121 tttactctaa ggagatgcgc aagacagttg aaaatcaaga aaatctgacc cttcgtcaaa
     3181 ccatgattga taagattttg gtggaagatg gcaaggttgt cggtgttcgt acagcgactc
     3241 atcaagagta tgctgctaag gctgttattg tgacgacagg gactgctctc cgtggggaaa
     3301 ttatcatcgg agacctcaag tactcatcag gtcctaacca cagcttggct tctattaacc
     3361 tagctgacaa tctcaaggaa ctgggtctcg aaatcggtcg tttcaagaca ggaacccctc
     3421 cacgtgtcaa ggcctcttct atcaactacg acgtgacaga aatccaacca ggagacgcag
     3481 tacctaatca tttctcatac acttcacgtg atgaggatta tgtcaaagat caagtgccat
     3541 gctggttgac ctataccaat ggtaccagtc atgagattat tcaaaacaac ctccaccgtg
     3601 cgcctatgtt tacaggtgtg gtcaagggag tggggcctcg ttactgtccg tcgattgaag
     3661 acaagattgt gcgctttgcg gacaaggaac gtcaccaact cttccttgag ccagaagggc
     3721 gcaatactga ggaagtctat gttcaaggac tttcaaccag tctgcctgag gatgtccagc
     3781 gtgacttggt tcattccatc aaaggtttgg aaaatgcaga gatgatgcgg acaggttatg
     3841 ctattgagta tgatatggtc ttgcctcatc agttgcgtgc gactttggaa accaagaaaa
     3901 tctcaggtct cttcactgct ggtcagacaa atggaacgtc aggttacgaa gaggcagcag
     3961 gccaagggat tatcgcaggt atcaatgcgg ctctaaaaat ccaaggcaag cctgaattga
     4021 ttctaaaacg aagtgacggt tatatcgggg tgatgatcga cgacttggtg accaagggaa
     4081 ccattgaacc ttaccgtctc ttgaccagtc gtgctgaata tcgtctcatt cttcgccatg
     4141 acaatgctga tatgcgcttg actgagatgg gccgtgagat tggccttgtg gacgatgaac
     4201 gctgggctcg ttttgaaatc aagaaaaatc aatttgataa tgagatgaaa cgcctagaca
     4261 gtatcaaact caagccagtc aaggaaacca atgctaaggt tgaggaaatg ggcttcaagc
     4321 cgttgacaga tgcggtgaca gccaaggaat tccttcgccg tccagaagtt tcttaccaag
     4381 atgtggtggc cttcatcgga ccagctgcag aggaattaga tgacaagatt atcgaattga
     4441 ttgaaacaga aatcaagtac gaaggctata tttccaaagc catggatcag gttgccaaga
     4501 tgaaacgtat ggaagaaaaa cgcattccag ccaatatcga ctgggatgac atcgattcta
     4561 ttgcgacgga agctcgtcag aagttcaaac tcatcaatcc agaaaccatc ggccaagcca
     4621 gccgtatttc gggagtaaac ccagcagata tttctatttt gatggtgtat ctggaaggta
     4681 aaaatcgtag tatttctaaa actcttcaaa aatcaaaatg atacgtcgta ggcttcttac
     4741 gaatgagttc aaagcttggc tttgattcat ctccaacctc ccatagttcc ccgaactatg
     4801 ggagctaact caagttatgc attttcctag ccttctggtc tgattttcat tgagttttaa
     4861 tctaaaaaga aagcatagag aaaaacagct ccgaagacgg ggctgttttg ttgatttgta
     4921 ctcaatgaaa atcaaagagc aaactaggaa gctagccgta ggttgctcaa agcactgctt
     4981 tgaggttgtg gatagaactg acgaagtcag ctcaaaacac cgttttgagg ttgtggatag
     5041 aactgacgaa gtcagtaaca tatatacggc aaggcgaagc tgacgtggtt tgaagagtat
     5101 tattcttcat ctggtgtgag gatcatcggg atgatgatcg gttcacgttc ggtattttca
     5161 tagaggaagg ggcgaatagc gttgacaatg gcaccattga cagattgcac gctagcatcc
     5221 ttatttttca gtgcgatacg aatggcattg aagaggatac gctggctttg acgaatcaag
     5281 tcgccagact ctctcatgta gacaaagcct cggctgagga tgtctggacc agatagaatc
     5341 atctgcgatt tgaagtcaac agttgcgact gccagaacga caccgtcttc agatagatcg
     5401 cgacgatctt tgaggacagc tgcgccaatt tcaccgatac gatttccatc gacatagata
     5461 tcttgggcgt tgaaatggcc tgcgatacga gctgagtcag cagtaagggc aagcacatcg
     5521 ccattgctca tgataaagat attgtccttc tcaacaccag tatccactgc tagtccagcg
     5581 tggacttttt gcatgcggta ttcaccgtgg acaggcatga agtattttgg cttaatcaag
     5641 cagagcatga gtttttgctc ttgctgacca ccgtgtccag atgtatggat attgttcact
     5701 ttaccgtgga taacttcgac accagcttca gaaatgatgt taatcagctt gttgacgcta
     5761 gtagtgtttc cagggatggg actagaagag aagataacgg tatcacctgg ttgtaattgt
     5821 acttgacggt gggttccgtt ggcgatacga gagagggctg ccataggctc accctgacta
     5881 cctgtacaga ggataagaac ttctcctgca ggataatctt tgatttcatt tggctcgata
     5941 aaggttccct taggagcttt gatgtagcca agatcgattc cgttgacaat ggccttttcc
     6001 atagaacgac caaagaccgc aatcttgcgt ccagtcttaa cagcagcttc tgttgcctgc
     6061 tggagacgga agatatttga ggcaaaggat gcaaagatga tacgtccttc aataccttgg
     6121 ataatcttca taatggactg accaacgact ttttcagagt tggtaaaggt tggtacttcc
     6181 gcatttgtcg agtcagacag gagacagagc acgccttctt caccaagcgc agccatacga
     6241 tgcaagtccg caggttctcc aactggagta aagtcgaact taaagtcacc cgtacagacg
     6301 attttccctt gaggagtatg aatgacaatc cccaaaggct ctggaataga gtgagtcgtt
     6361 ctaaagaaag ttgccttgag atttttaaag gtcaactcgg tgttgtggtt gatttcgtaa
     6421 agtttggcgt tgcgcaagag gccgtgttct tcgagtttcc cacggatcaa agccaaggca
     6481 agcggtccag cataaatagg gacatttgct tgcttgagta ggaacggaat cccaccaatg
     6541 tggtcctcgt gtccgtgtgt gattaaaaca gccttgacgc ggtcgatatt gtctacgatg
     6601 taagagtagt caggaatgac atagtcgata ccaagcaagt catcttctgg gaatttaatc
     6661 ccagcatcga cgatgataat ctcgccttgg tattcaattc cgtaagtgtt tttcccgatt
     6721 tctcctagac caccgatggc aaaaacgccg acttcttcag gtttaagagt gtaggccata
     6781 ttagaactcc gtaatttcga aggcgccagt ttctttttcg taatcgagca atttgtcaga
     6841 caagagttcg atatattcga tattgtactc tgggcgattt tcttcgacaa gttggcgagc
     6901 agtgatacgg ccctcaagtt ctgagctggc atcgatgtct aggtaaagcg cgcgtgttgt
     6961 ttcacggcgt gggctacgtt cttttgtttc ttgataaaaa actttgtaaa tcatatagtt
     7021 cctttctatt tacaggtcag cttattccaa acttttagca gatatttgct tgatttcatt
     7081 ccattttgtg tctagattga ccttgattcg ttacaattat ttcaattata ccacaaaatg
     7141 aaaaattagt aaaagacgga aacgagaaag ttaggggacg atttcttcgg taagcgtttg
     7201 cgaaaataaa gaaaatgtat tatgttgata acggaagtga aatttattgc ttgtcataat
     7261 tccatataga ttttaagtca aggaggttgc cctatgtata acttatttgc ttttgctatc
     7321 ggagttcgtt tgctagcttt tatcgttttt gttttgcgtt tatggaggaa atcttctatt
     7381 tgccaaactg gaacgatgac aaaccaagtt cttgaggaaa attacctttg tgactctcta
     7441 ctcgcttttt ctcctcctcg tgacctatgt cgtttggttt gtattttact ttggattaaa
     7501 tgcttagatg ccctgcttat gctggggttt tttgtttggg gatgtagaga tagtagattt
     7561 acgacacgaa aattccaccc catctatcaa gatgaggtgg tctttgcatt tttctaaaat
     7621 gatatgacaa tggaattttc aaagcttatt atttttatgg gagtcattgt tctgctcaaa
     7681 aatagagttg aagcataaaa ataaaacaat acaaacagat aaatcgaatg gtaaagggat
     7741 ggtagtaatg ttcaaatcat tgagaatgct gagcaaactg taagagatga agacaataaa
     7801 atttatcctt ctaaaaatgt gtttcttatc tagcatgact taccaaactt tttacgaagg
     7861 tcttttccaa tggtataacc tgccataaat aaagcgatac caacacctac accaaagcca
     7921 cccttgattt cttgcaattc cttattagaa atttcacgac agttgttcaa atctttcatc
     7981 atattaatct cctcctaaaa aataaccagc tgccaaacca gaacctataa gtgctgttcc
     8041 acctgcaata gctttccaag atactgctac tccaaagata accaagggag cgagaccgcc
     8101 atcaatatct gataattcat tttcagataa ggataaaaat tgattgtcaa gaatgtcaaa
     8161 atttgtcatt gaactttcct ccttttttct tgttcacttt tatattcgga aaagaaaagg
     8221 aaaagtgagc atttttttaa attattttta gttaagtgag ggcactcttg tctgggggca
     8281 aagaaaattc ccatgtgcca gcttttgtag tataatagta agcagacaaa aagataggaa
     8341 tgtgttatga aagtattagc ttttgatacg tccagcaagg ctctttctct cgctatttta
     8401 gaggacaagc aggttcttgc cgagacgacg attaatatta agaaaaatca cagtattact
     8461 cttatgcctg ccatagattt tttgatggca agtttggatt ggacacccaa ggatttggac
     8521 cgaatcgtgg tagctgaagg gccgggtagc tacacaggct tgcgaattgc ggtagccact
     8581 gccaagactt tggctcatac tctgaacatc gagttagttg gtatgtctag cctcttggct
     8641 ctggtaccct accaacaaga aggcttgttc gtctctttga tggatgcgcg tcgcaacaat
     8701 gtttatgtag gattttatga aaatgctaaa cccgtcatgc cagaagctca cttacccttt
     8761 gagcgagtga ttgagttaat caagggtgct agtcaggtta cctttgtcgg agaagttggc
     8821 ccctttgttg agcagattca agaatacttg ccaaggacta atttcaaaga aaccttgcca
     8881 aatgcagcta atctagctct atgggcctgg gacaaggaag cagactcctt gcatgatttt
     8941 gtgccgaact acctcaagcg tgtcgaagct gaggaaaact ggctcaagaa tcacaccgag
     9001 tctggcgagt cttacattaa acgcttatga tagaaatcaa acgaatccaa aaacattctg
     9061 acttggctca agccatctac gctgttatga tagctgttta cccagtcagt ccttggacgc
     9121 tggaacaaat ccaagcagac ctgtcccaag accaaacttg gtatgctttg gcttatgatg
     9181 gggcagaagt gattggattt ctagctgtgc aggagaatct ttttgaagca gaagtcctgc
     9241 aaatcgctgt caaaggagct tatcagggtc agggaattgc gtcagccttg tttgctcaat
     9301 tgccgacaga caaggaaatt ttcctcgaag tcagacagtc aaatcaacga gcgcaagcat
     9361 tttacaagaa agaaaagatg gcagttatcg ctgagcgaaa ggcctactac catgacccag
     9421 tcgaggacgc cattatcatg aagagagaaa tagatgaagg atagatatat tttagcattt
     9481 gagacatcct gtgatgagac cagtgtcgcc gtcttgaaaa acgacgatga gctcttgtcc
     9541 aatgttattg ctagtcaaat tgagagtcac aaacgttttg gtggcgtagt gcccgaagta
     9601 gccagtcgtc accatgtcga ggtcattaca gcctgtatcg aggaggcatt ggcagaagca
     9661 gggattaccg aagaggacgt gacagctgtt gcggttacct acggaccagg cttggtcgga
     9721 gccttgctag ttggtttgtc agctgccaag gcctttgctt gggctcacgg acttccactg
     9781 attcctgtta atcacatggc tgggcacctc atggcagctc agagtgtgga gcctttggag
     9841 tttcccttgc tagccctttt agtcagtggt gggcacacag agttggtcta tgtttctgag
     9901 gctggcgatt acaagattgt tggggagaca cgagacgatg cagttgggga ggcttatgac
     9961 aaggtcggtc gtgtcatggg cttgacctat cctgcaggtc gtgagattga cgagctggct
    10021 catcaggggc aggatattta tgatttcccc cgtgccatga ttaaggaaga taatctggag
    10081 ttctccttct caggtttgaa atctgccttt atcaatcttc atcacaatgc cgagcaaaag
    10141 ggagaaagcc tgtctacaga agatttgtgt gcttccttcc aagcagcagt tatggacatt
    10201 ctcatggcaa aaaccaagaa ggctttggag aaatatcctg ttaaaaccct agttgtggca
    10261 ggtggtgtgg cagccaataa aggtctcaga gaacgcctag cagccgaaat cacagatgtc
    10321 aaggttatca tcccccctct gcgactctgc ggagacaatg caggtatgat tgcctatgcc
    10381 agcgtcagcg agtggaacaa agaaaacttc gcaggctggg acctcaatgc caaaccaagt
    10441 cttgcctttg ataccatgga ataaagagtg gctctttgtc aagtgtagtg ggtaggcgaa
    10501 aagctacaat ctggagagta cgaaattcgt actctctttt ttgatgttca aagtcatcaa
    10561 aatccgtttt ttaaaattgt taaagttccg aaaaccaaag gctttgcgct tgataacctt
    10621 aataaaatta ttggtcgctt caagtttagc gttggaataa ggcagcttaa gtgcgttgat
    10681 aatcttatct ctatccctta aaaaagtcca aaagaccgtt tgaaaaagcg gatgaaccgt
    10741 tggcagttct tgctcaatca atccaaagaa atggttggca ttcttctctt gaaaatgaaa
    10801 gagcaggagt tgatagagtt cgtagtgaac ctgtaatccc tcagagtaag aaagcagctt
    10861 atctcgtact tcccgatggg ttaagtgcat tcgaaacgtg ggctgataga atcgtttctg
    10921 acagagcttg aagctatcct ggtgtagtaa atcagtccag tggactgatt tagcccgagc
    10981 ctagaaacga aaaagcgagg gttccaaaag cgcttgagcg cccgatactc caaggatttt
    11041 cggtcaaatt ggttcatgat ttggattcgt actcggttca tagctcggct cagatgttgg
    11101 acaatgtgga ggcggtcaag aacaatcttc gcctttggaa agagttgctt agcgagccga
    11161 taataagggc tgtacatgtc catggtgatg acctcgacca gctcccgaac ctctctctga
    11221 tagcgttgga aatggtttcg aatcaccgca tgagttcgcc cgtctaaaat tgcgaggatg
    11281 gatttggact caaaatcttg ggcaatgaag ctcattttgc tctttttgaa gctatactca
    11341 tcccaactca taacttttgg caacttggtc caatcggttt caaacttaaa ttccctcagt
    11401 tttcggatga cggtggaagt tgagaccgcc aatctgtggg caatctcagt catagactgc
    11461 ttttcaagga ggagctgagc gattttttgt ctcaccatgt tggaaatctg gcaatttttc
    11521 ttgacaatgg atgtttgaga aacgaccacc ttaaggcaat tcttgcactg aaagcggcgc
    11581 tttttgagat gcagtaccgt gggtaaaacc tgacagtcga gaagcggaat tttagaggct
    11641 ttctggaagt cgtatttgat catcttccct tgacaatgag gacaaggagg agcgggggaa
    11701 tccaactttg cctgaacgac tagatgggtt tggtatttca gaacagacaa gattttgata
    11761 tttttgtctt ccaatccgag caaatctgtg gtattcttaa gttgttccat atgaaccctt
    11821 tctaatgagt tggttagacg cttttcatta taggtcatat ggggcttttt ttctacaaga
    11881 aacgacccta taattcctgg ggtgggatta cccactacag aaattataga gccaaagcat
    11941 tccaaagtct tgtctgactc tggaatgttt tctgttcaca gttgttttgt aactgcctaa
    12001 taagtaaccg cgctataacg aggtatctac cgagattgtc aattctccag tatactgtta
    12061 taaaaaacaa aactggagga tttttttatg cccccccatc gtcccatcga ctgttccgta
    12121 gctgcccaat aacggagtat aatcagctga aacaagaata ggacaaaagt gcctcaagaa
    12181 aagcatcgta atttgacaat attattctga gatgtttttg atgtaagccc atattttctc
    12241 aatgggatta tactcgggtg aatagatagg aagtggtaac agtctatgcc ctgctcctta
    12301 cacatgttca ttctgtgaaa ccttgcattg tccataatga taagggatgg tgtatctaaa
    12361 gtaggtagta agaatttttg gaagcaagct tcgaaaaagt cactcgtcat cgtatcttta
    12421 taggtcatcg gagccatagt atattgaaac aaaaaagggc attagtactt ctataacagt
    12481 atcgcaatct ctggtgtgcg ttgtctcgat cttaattcaa ataattatat cttcttccag
    12541 agagactaac tgcctgctca aggctcgatc atattcgaga taaaaatagg tcttaacccc
    12601 tgtctcataa atataaatag gagtcaggta gcttaagtga ttcaattctt taaggaaccg
    12661 atttactttt tctgggtctt gttcgcagta ggtacggctc ttttttagac tatatcccat
    12721 agcctttaga gcgtaatgaa tagttgttgg aggacagttg aattcagcag ctatttcagt
    12781 caaataagca tcgggatgag tttcaatata gatctttaat ttatctctat caactttttc
    12841 aattaatagt ggaagttttc ttaaagttag tgtattgaaa cgtttttata cagtgctaaa
    12901 atttgaagtg tagtgcagat atatttgtaa tagtattata gaaattcttt gaagaaggtc
    12961 gagacaagag aaaataggta
//
LOCUS       DAVLSL010000045        12873 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLSL010000045 DAVLSL010000000
VERSION     DAVLSL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.046"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..291
                     /locus_tag="VXN30_001025"
     CDS             <1..291
                     /locus_tag="VXN30_001025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357663.1"
                     /translation="EVSSKHGFEVVDETDEVSSKHGFEVVDETDEVSSKHGFEVVDET
                     DEVGSKHGFEVVDETDEVSSKHCFEVVDETDKVSNHIYGKATLTWFEEIFEE"
     gene            complement(348..830)
                     /gene="greA"
                     /locus_tag="VXN30_001026"
     CDS             complement(348..830)
                     /gene="greA"
                     /locus_tag="VXN30_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1357664.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(901..2556)
                     /gene="mltG"
                     /locus_tag="VXN30_001027"
     CDS             complement(901..2556)
                     /gene="mltG"
                     /locus_tag="VXN30_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1357665.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPAKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2637..3131)
                     /locus_tag="VXN30_001028"
     CDS             complement(2637..3131)
                     /locus_tag="VXN30_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357666.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVDLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(3128..3592)
                     /locus_tag="VXN30_001029"
     CDS             complement(3128..3592)
                     /locus_tag="VXN30_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357667.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKVIRLESPDELLSYFGMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3602..4936)
                     /locus_tag="VXN30_001030"
     CDS             complement(3602..4936)
                     /locus_tag="VXN30_001030"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1357668.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4947..5564)
                     /locus_tag="VXN30_001031"
     CDS             complement(4947..5564)
                     /locus_tag="VXN30_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1357669.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKLVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5612..8710)
                     /locus_tag="VXN30_001032"
     CDS             complement(5612..8710)
                     /locus_tag="VXN30_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1357670.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKVLPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREKDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(8803..9969)
                     /locus_tag="VXN30_001033"
     CDS             complement(8803..9969)
                     /locus_tag="VXN30_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1357671.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSIKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(9977..11071)
                     /locus_tag="VXN30_001034"
     CDS             complement(9977..11071)
                     /locus_tag="VXN30_001034"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1357672.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(11228..12871)
                     /locus_tag="VXN30_001035"
                     /pseudo
     CDS             complement(11228..12871)
                     /locus_tag="VXN30_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         3673 a         2902 c         2407 g         3891 t
ORIGIN      
        1 gaagtcagct caaagcatgg ttttgaggtt gtagatgaaa ctgacgaagt cagctcaaag
       61 catggttttg aggttgtaga tgaaactgac gaagtcagct caaagcatgg ttttgaggtt
      121 gtagatgaaa ctgacgaagt cggctcaaaa catggttttg aggttgtaga tgaaactgac
      181 gaagttagct caaaacactg ttttgaggtt gtagatgaaa ctgacaaagt cagtaaccat
      241 atatacggca aggcgacgct gacgtggttt gaagagattt tcgaagaata gcaaaatgga
      301 aaaaggagtg agtgaagcac atcgcctccc cactcctttt tctgttttta ggctgttttt
      361 tcaaccttca agatttttac atcatagcta ccaacaggcg tttcaattgt tgctgtgtca
      421 cctgttttct tgccaatcaa ggcctgccca attgggcttt catttgaaac cttacctgca
      481 aaggcatccg caccagctga acctacgata atataaactt cttcttcgtc ctcgccaatt
      541 tcttggatgg tgactgtttt accaatcgct acttcgtcct gggcaactgc gtcgctattg
      601 acgatttcag catagcggat ttttgtttct aagctagaga tttgtccttc gacaaaggct
      661 tgttcatcct tagctgcttc gtactcactg ttttctgaaa gatcaccgta tgaacgggca
      721 atcttaatgc gttctaccac ttctggtcga cgaaccaatt tcaattcttc taattctttt
      781 tcaagtttct ccttttcctc aagggtcata ggatatgttt tttctgccat ttttctcaac
      841 tttcttctga taatattttc taaagaaaat tatgtgaaat atcacataat tttagtttgt
      901 ttagtttaat ttgctgttta catgttcagc gacattgcgg tcgtggtctt cttgattgtt
      961 agcatagtag accttgcctt ctgtgacatc tgctacaaag tagaggttat cgctcttagt
     1021 ttgattgatg cttgactcaa tcgcatccag acttggacta tcgactggac caggcatgag
     1081 acctacattt ttataaacat tataaggtga atcaatgttg gtatcaatcg caacatcctc
     1141 agctagactg atattttgcc ccagttttcc ttgggcatac aagattgcaa tattactttg
     1201 aagtggcata tcacgattca aacgattgta gaatacacct gcaatgagct tacgatcttc
     1261 tgtcttggca ccttcttttt cgaccaagga agcaatggtc aacaactcat tgacagtcaa
     1321 gtttttagat ttgatagtac tatagtaagg agataggttc ttatccatag cagctaacat
     1381 ctcatcaatc aagctctcaa tagttgtgct ttccttgata gagtatgtag ctgggaaaag
     1441 gtatccttcc aaacgataac gcgcaccgct gtcttttaca ggcaaacttt ccagtaaagt
     1501 aggatatttc gctactgctt gactgataaa cgtctcatct tgaactttag ccaagaaagt
     1561 ctccgctgtc aaagactctt tgaagtcacc ttgcaattga cccacagctt gagcaatctg
     1621 atccaaggta taaccttctg gaattgtcaa agtcgcaagt acaggttctt gcggttcatc
     1681 tgttccacct ttttgcaact ctttgagtaa gtcttctgta ctcatactct tttgcaaatt
     1741 gtagtaacct gctttcaagt cggtataatt tttatacttg gcataaaaac taaaaatcag
     1801 accatgcttt accaaaccag ctttttcaag cgtcgtaccg atttcttgaa cgtttgaacc
     1861 ttctggaatt ccaaccgtca catatttctt agaattagca tcgataggta ataaggaatc
     1921 tagcacgtac tggtaaccaa agtaaccacc tgctgagagc aaggcaagga atacaaggaa
     1981 tgaaatcaca aaagctttca catgtgactt cttctctttc ttaggcttag cgggttttgc
     2041 tccttctcta cggctacgac gtgatgtatc tctgatgata tccactgttt ctgtagcagg
     2101 cgtaggtgtt tctggacctg cttgttctgc ttttttctct gccgtcgtat aggaaacagc
     2161 tacccttgtt ggggtttcat tgtattctct ttcaagtttc ttaggtctaa caggacctgg
     2221 acctggtctt gatccacttt cttccgctgg agaagaaggt acatcttgac ttggatgact
     2281 tggaacacca ggagtttctc tttgaatctc atctgctgga gaagctggta caccttgact
     2341 tgggtgagta ggcacggtag gagcttttct cataatctcc tctaccgttg acaaggaatc
     2401 agccatgagt tcttcagctg aaggttcatt tgcaggagtg cgaactactg cctcatcttc
     2461 tttcagaact tcatcatagc cttttacttt ttctaaatct ctcagaatct gctctttaaa
     2521 gcttaatttc tcttcttctc ttgacttttc actcaaaagt ttttcctcct tgttgataat
     2581 ccataatatt atatcttaaa agtccaaaaa aagcaatcta tgatactttt cctaacttat
     2641 tttttcattt cccagaccat atcataccat gtttcccctg caaaggttga ctgggactgg
     2701 ccttcgttga caaatccatg tttttcataa taggcgatga gatagtcatg acaggttaga
     2761 ttaatgccat ctctctcatc ttcaagagca acttctttca aggctgtcag caatttctga
     2821 cccagtccga gtccctgtgc ctccttggca atagacagac aggtcacaga gatataacca
     2881 ccaggctcat gactatagtc ttttatttct tctgtaaaag actggtcttg cagatggcgg
     2941 tgcaggtcaa ctggtccttc gatataaccc atgattcttc cttctttttc agcaaccaga
     3001 aaagaggtct gaatttctct caaatgtgct tcaaagacag aaggaggaat ggcttcttcg
     3061 accgaaaaat tttcaaattc aagttcaaca atccgatcca aatcttctaa tcttgcttgt
     3121 ctgattttca ttgttcctcc agataaaagg gattaaacca aatcatacta tagccctggc
     3181 tagttgcata gagcaaagtt tcttcttcat caacaaaacc gttcattcca aaataggaaa
     3241 gcagctcatc aggactctcc aaacgaatca ctttgtaatc cagctcaact gccacctctt
     3301 tcaaggctgc aagaagaagt gttcccaggc cctgtctctg atggtcagac tcgatgacta
     3361 aagaatgtac ttttagacat tgcggattgt ctgactgggg acttgataaa atatagccta
     3421 aaagttgatt ttcatcccta gctagaagaa aggtatccgc acacttacgg atactttctt
     3481 ctaaaatatg ggaaagttgc tgcttttcag ctggaaaaga cgaggtctga agtgccccta
     3541 tctcaggcaa atcagacttg cttgcctgaa taatcttaat tggaatttcc atgggaacat
     3601 cctattgaac attgcttgtc aagttagaca agagacgctc aaatgagtat tcataggttt
     3661 ggatgtctcc tgctcccata aagacgtaaa cagcattgtc atggtctagg agcggagaaa
     3721 cattttcaac agtaatcact tggtgttttt tgttgatttt gttggctagg tcttctacct
     3781 taacgtcacc atgatctact tcacgagccg agccataaat ttgcgctaga taaacagcat
     3841 ctgcttggtt taaagcatgg gcaaagtcgt ccaacaaggc aatggttctt gtaaaggtat
     3901 gcggttgaaa gactgctaca atttccttgc ttgggtattt ctgacgagcc gcatccaagg
     3961 tcgcaataat ttctgttgga tgatgggcaa agtcatcaat aatcactgta tcattgacaa
     4021 ttttctcagt gaaacgacgt ttaacaccgg caaatgtttt caagtgctca cgcaccaagt
     4081 tcaaatcaaa tcctgctgtg taaagaagac caataacggc tgtcgcattc atgatattgt
     4141 gacgaccaaa ggttggaatg tggaattgcc ccaagttttg tccacggaaa tgaacggtga
     4201 aggttgaacc agttgttgaa cgaagaagat cactagctac aaagtcattg ccttcagctt
     4261 caaaaccata ataataaatt ggtgcatcag acgtaatctt acgcaattca gcatcttcac
     4321 catagacaaa aagacccttg gtaatttgtt tggcatagtc gttaaaggca ttgaaaacat
     4381 cctcgagact tgtgaaataa tctggatggt caaagtcaat gttggtgata atagagtatt
     4441 ctgggtggta aggcatgaag tgacgctcat attcgtcaga ttcaaagaca aaatatttgg
     4501 cattagccga accacgacct gtcccatctc caatcaagaa gctggtatct gtaatgtgag
     4561 acaagacatg agacaacata cctgtcgttg aagtttttcc atgtgctcct gctactccca
     4621 tgctaacaaa gtcacgcata aagctaccta gaaactcatg gtaacgtttg tagctgatac
     4681 cattttggtc cgcataggca atttcgacgt tgttatctgg acgaaaggca tttccagcga
     4741 taatttccat atcaccgtct agattctttt catcaaaagg aagaatggta attcctgcct
     4801 gctcaagacc gcgttgggta aagtagtact tttcaacatc tgatccctga accttgtgcc
     4861 ccatctggtg caacatcaag gccaaggcac tcatccctga tcccttaatt ccgataaaat
     4921 gatatgtctt tgacatgttt tctcccctat tctgtcattc tggtcagatt caactcttgg
     4981 gcaacccgac gttcttgttc tgtttgttta ctttttttat tgtagatttg gctcttcttt
     5041 agaaaatcat aattgttttt ctttggagca ggtgctgaca cttcttcatt cttggtaggg
     5101 atagaatgaa cttcttccgc caagatataa tgagactggg tcaatttttg gctatatttg
     5161 acaaattcac caggattttc cttttggaaa ggagttgtcg gttgattgcc ctgtctaact
     5221 agactgggct gagaatgacg tctcgcaagg ctgaaatcct gagttaggta gttagcagag
     5281 cgtttctttt tcaagtccgc acgcgcttct tcacgcgcca cctccgcata gctctttcct
     5341 tcttttttaa cccctaaagg agccttttta ggtttttcga cttgcttttc aatcggtttt
     5401 actagttttt cttcagtaat aggagcccat tctaaataat ttttatctcg atactcaccc
     5461 ttgatattac tgatcagatc agactcatca tagagattca tgactggcat ttcagtcaac
     5521 atgacctcgt catctgacac caatggaaat cgttcttgtt tcattttcta tttcctttca
     5581 acacttcatt atagcgtatt gtcttgattt ttcaagtgct ggcttcagaa attcccaaaa
     5641 tttctctaat ttctgctagg gtcagactac cacgtgactc tgtaccatcc aatacttgtg
     5701 acaccagatg tttcttttgt tcttggagtt cctgaatttt ttcttcaatg gttcccttgg
     5761 tcaccaagcg atagacctca accgtttctt cctgacccat ccgatgggca cggccaatgg
     5821 cttgcgcttc caccgcagga ttccaccaaa ggtcaaccaa gatcactgta tctgcacctg
     5881 tcaggttcag accgacccca ccagccttga gggaaatcag aaaggcatct ctttctcctt
     5941 ggttaaaggc cttggtcatg tcttgtcttt ccttggctgg ggttgaaccc gtaattttaa
     6001 aggatgtcag gcccaagtct ggcagttctt gttcaatttt ttccaacatt cccttgaact
     6061 gagagaaaat caagacacgg tgtccgccgt ctgccacctg taccagtagg tctcggagac
     6121 tatctagttt gccgctggct ccctgataat cttccataaa cagggcagga gtgtcacata
     6181 tttgacgcaa gcgcatcaaa ccagataaaa tttccacacg acttcgctga aattcctgtt
     6241 ctgacacttg agccagatgg tctcgcatct gttgtaactg ggcaaggtaa atagcctttt
     6301 gctggtcttc cagttcattt ttataaacca cctcaatcaa gtctggcaat tcagtcagaa
     6361 cttcttcttt cttgcgtcgc atcacgaaag gcttgataaa ctgagccact cgctctgctg
     6421 gcaatttcat aaattctttc ttgcttggca aaagtccggg catgacgatt tggaaaatag
     6481 accacaactc acccagatgg ttttcaatcg gagttcctga caaggcaaag accgacggca
     6541 ccacaaattg tctcaaggtc tgggcaatct tggtctgggc atttttcatg acctgagcct
     6601 catctaagaa aaggaagtca aaggccatcc cctgataaaa ctcactgtcc tgacggaagg
     6661 tggcatagct agtcacatag atttgatggc tctcggcaag aatctcctca cgacttgctt
     6721 tcaaaccatg aacaacagcc acatccaact gtggggcaaa tttctgaaac tcatctgccc
     6781 agttgtaaat caaacctgac ggagcgagaa tcaaaacccg actttctttt gtcacttgac
     6841 tagtcaaaaa agcaatggtc tgaagggttt tcccaagtcc catatcatca gccaaaatcc
     6901 caccaaaacc ataatgatgg agcatctgca accagccaat tcccttttcc tgataatctc
     6961 gcaagtcagc ctgaacctga gttgcctgtc gaggaaagtc ctctggatgt gtcaaatcct
     7021 gggccagatt ctggaattct tgtgaaaaag aaacacggtc tcgcccttca aagagatgag
     7081 ctaaactgta ggccaaggat ttccgagcct gcaaggtccc atcttttaat tcaaattgcc
     7141 ccagttcctg tagattttgc cgaattttct tggtttcttc atcgaaaaag taaacttgat
     7201 tagacgaatc aatataaaaa tcctgattgg caaccaaggc ctgcatggct tggtcgattt
     7261 cctcctgggc aatattttga aaatcaaact ggatttccaa gagacctccc ttggaggcaa
     7321 tctgcacctg aggactcgct aggctataaa gctcttctag tttatctgat aggtcaacat
     7381 gcccgagttt ttcaaagact ggaatgatat catgaaaaaa atgatagaca gactccgctt
     7441 ttaaggcctg acgccaagat tgaaaatcgg cttcaaagcc cgcagccaaa cagacttgga
     7501 aaattctttc ttctaagtcc gcgtcacttg aaaatggtaa ttcttctagc tcttgtcggc
     7561 tagatacctg tctatttcca taatcaaact gaatttctaa acgaatccga ttatcttttt
     7621 ccctgtcaaa gtaaaaagag ggcgcaaaag ttttgatttg tagacgttct ggagctgaaa
     7681 cggtgcccat ctggataaaa agagtcagac aggaggccaa tttgtctcga tcactgctat
     7741 caaattgcaa gtatttcttt ccttgttgac ccacaggtaa cactttaatt tccttgagaa
     7801 gacgcatctg ctggtctgtt aaaaaataaa cctgaccttt atggaaaagt actgctccct
     7861 gataaaagac attaacccta ggactctcac tgatttccat ttcaaaataa tccgagtatt
     7921 ctgttactgt aaaggcaaat agattggcat cagcatgcat atcctgaaaa agcagggttt
     7981 ggtagctatc cacttgatgg tcaaattgaa aatggggcaa ggccatcagt aaattcacac
     8041 cctgctcaaa aaaggtcaga gggaaaaaga ggtgccgacc ttggttttgg aaaaagaggt
     8101 ctggaaccag cccttcctcc gttagtccgt gcaagaaagt caaaagttct tggctggcat
     8161 catcaaaggc ttcccaagaa agagactcct cataaatctt gccaatcata tacgactttc
     8221 tctgctcgac aatccttaaa aaaagtggaa tatcccgaat gacatagtat ttttggctat
     8281 tgatttggcc gattctcaga gtccacaaga tatgattggt tcctgcttcc acctgaccca
     8341 cagctgataa ctcataggcg cattctgatt ttggagataa aattcgatcc aaaaacttgc
     8401 cacccaaggt caccttggtt tcaacagcct ctttttcttc atgaccttct tccagactcc
     8461 acaagatttc ctgaccacgc tcatcatttt tcagaaaatg ctctagcgct gccaaatgca
     8521 cacagtagcc cctcttttga aaaaaatcac aggcacaaaa aaccaaatca tcctctaaac
     8581 tatagcgcag ttcttcttct gcaacgcgag cgtagagccg attgttcttt tccttgatga
     8641 tatcaacctt accagtttca taaagggcaa caccttcgat acgaactttc cccggaatca
     8701 atttagccat attttcacct ttaccttatc tttttattat accatatttt ctcctatgaa
     8761 aatagccttc taggaagact tttctcctag aaggctggat ttttaacgtt tggcaaaagt
     8821 agccacaatc cgctgacaga cttcttgcaa cagagatttg ggcatagcta tattgatgcg
     8881 ggcatggaga cttccttcct ctccaaaatc caaaccacgg ttgaggataa ccttggcttc
     8941 atttctcaac aactcttgca atgtttcatc agtcaggtca taagctgaaa agtcaagcca
     9001 aatcaagtag gtaccttgcg gtttcatgac cttgatttta gtctcttttc caaatagatc
     9061 caccacataa ttgatgtggt cttcaaagac ttgcttgagt tcctctagcc aatctttacc
     9121 gtatcgatag gcagcttctg tcgccaaata acccaagcct gaaatttcat gctgattatt
     9181 ggccaacagg cgtttctgga aagccagtct caacttagga ttttcaatga ctgcatagga
     9241 attttttgtt ccagcaatat taaatgtttt agtggcactg ctcaagacga tagcaaaatt
     9301 tttgaaggca ggattgatgg tattgaaaga ctggtgtttg tgaccaaaga gggtcaaatc
     9361 ttggtgaatc tcatccgaaa ctaacaaaac accgtgtttt tggcagagtt ggccaatctt
     9421 ctccaacact tctttttccc aaacacgtcc accaggattg tgagggttgc aaagaatata
     9481 gagtttaacc tcctcttcca ccaaatcctt ttcaagttgg tcaaagtcaa tctcaaacag
     9541 accatccttt tccactaagg aattagtaat caatctacga ttattcaact tgatactgcg
     9601 agcaaagggt gggtagacag gcgtgttaat taaaaccgcc tcgccttctt ttgtaaaggt
     9661 ttgaatagct gttgagatgg ctggtaccac accctcgata aagacaagag cctctttgtc
     9721 aaagttgtaa ccgtattgtg tagcttccca cttttgaact tccttaatta agtcttcact
     9781 ggcataggta taaccataaa ccagttggtc tgcgtaagtt tgcacggctt ggcggatttc
     9841 aggcaagacc acaaagtcca tatccgctat ccaagctggt agaacttcac tatccgtttc
     9901 tgtttctttc catttatagg tatggtgccc taaacggttg ggcaggcttg taaaatcata
     9961 ttttcccatc tttgtcttat ccttctaagg cttggcgcaa atctgcaatc aaatctctag
    10021 catcctcaat cccaatagac aaacgcaaga ggtcatctgt caaaccataa gaatggcgta
    10081 cttctgctgg aatatcagca tgagtttgag tcgttggata agtaataaga ctttccactc
    10141 cacccaaact ttccgcaaaa gagaagacct tgagactgtt caaaatatga ggaatgcgtg
    10201 tttcatcggc tactttaaag gaaatcatgc ctccacgacc agtgtagaga acttccttaa
    10261 ctgctggaga atccttcaaa aaggcaacca cttcttgggc gttagctgtt gagcgctcca
    10321 tacgaagaga caaagtcttg agaccacgaa gcaactggta gctgtcaaat ggagacaaga
    10381 ctgcccctgt tgtattgaga ttgtaaaaaa gcttctcgta tagttctaaa ctattggtca
    10441 caaccactcc agccaagaca tcattgtggc ctgctagata cttggttgct gaatggagaa
    10501 cgatatctgc tccatcttca atcggacgtt ggtagatagg gctgtagaag gtattgtcca
    10561 ccaccacttt ggcaccctta gcatgagcca attttgctag tttttcgata tcaaattcca
    10621 acatcaaggg attggttggg gtttcgatat agagaacatc cacatccttt tctaactcgg
    10681 caatcaactc ttcttctgta ttggcatagg taaaatggaa atgaccttcc tgctccactt
    10741 ggttaaacca gcgaaaagaa ccaccgtaaa gatcacgcac tgccaagacc ttacttccta
    10801 ctggaaagac gctaaaggcc agtacaatag ctgacatccc tgagctagtc gctagggcat
    10861 agtctgctga ctcaatagcc gccaagactt cctcagcctt actacgagtt ggatttttag
    10921 tgcgcgtata gtcaaaccca gtagatcgac caaactctgg atgctgatag gtcgttgaaa
    10981 aatgaagcgg tgtcaccaat gcacctgtcg cttcatctga cttaataccc gcctgggcca
    11041 aaattgtgtt aatgtgtaat tccttgctca tacaatacct ccaaatctat agtaactatt
    11101 gtaccactta ttttcatcaa ctcattttct tcttttcaag agctagttat agtttcaaac
    11161 tatataaaaa gggagttttt cctgctccct ttaatagact ataaaatggt gaatctcaaa
    11221 agacacttca ctctatcatt tgctcctgca caaaacgagc ataacgctca tgattttcca
    11281 gtagttcctt atgagttcct gagccagtga ttttcccctc ctctaagaag aaaatacaat
    11341 ccacatcttt taccgttgac aaactatgcg ctataatcac aaccgtcttc tcctttaata
    11401 cagaatagag gctactgata atcgcatact cagaatccgc atcaagatta gcagtggctt
    11461 catcaaatat aagaatttca gcatctttta agtaggctct agctatttga agtctttggc
    11521 tttgcccccc tgacaagaat cgtccgcgtt caccaacttc agtatctagt ccctctttca
    11581 tggagcgaat ctcatcacct agtgatacta agtctagcac tttcatcaat tcatcatcag
    11641 ttactaagcg attcaaaccg agacaaagat tgtcacgaat actgccagat aagactgcat
    11701 tattttgtga aacccaagcg attttacttc tccattcttt taagttaaaa tcatatatac
    11761 ttgattgctc cattagaata tctcctgaaa gcggtttata aaaccgctct aacaaacgca
    11821 caatcgttga ttttcctgat ccagatggtc caacaaaagc aatttttttc cccttgaaaa
    11881 ttgaacaagt aatatccttt aagacaggtc gattttcatc ataaccaaaa tagacatggt
    11941 taaaattcaa cctttatcct gataccgatt ttcctccctc aaatttttct ttaggaactg
    12001 caagcaagtt ctccagtgca actgaagatc ccttgctcct agaataaaca gttacaaaat
    12061 tagctatatt actaatagga ttaagtaatt gaaagaggta aatcaaaaac gaaaccaagg
    12121 ttcccacaga tatatatcct gcgctgaccc gataaccccc ataggttagc atcacagcta
    12181 tagtcgcaaa gataaataag agagcaaacg gggtctcaaa agaagtaacc ctatctgatt
    12241 tcagtgaatt gttttgtacc ctttcaatac aattatccaa aacatcctgt acacttttct
    12301 ctgcttggtt agtcttaatt aattcatgtt cttgaatctt ttcagtcaat tgccctgtta
    12361 aaaatccgat ttcctcctgt aaacgacgac tatacttttc actgatattg gaaaggggca
    12421 agataataaa catcatacaa ggaagagtga tgaataaaag tagagaaaga ttccaatcaa
    12481 gactaaataa gactacaatg gaaccaagta ccataactaa actcagaata atatttggga
    12541 aagtcgtaat taaaaactca cgaatgacac tcatgtcatt gacaatggca gaagtcaact
    12601 ccccactttg gctcttatca aagaaggatt tctctgcata aatcaacccc tctatcactt
    12661 ttttcctgat ttttgctatc tttttttcac ccgattgact aaacagatag taaccaatag
    12721 aagaaaacaa ggcttgacca ataaaaatca aaaacgattg aaatactttg gaacctatat
    12781 tttcaataga actcccatct attaaacctt ttaagataag gggaagcaac aaagcaagta
    12841 gactagacag aacaaggaag aaactcccca taa
//
LOCUS       DAVLSL010000046        12620 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLSL010000046 DAVLSL010000000
VERSION     DAVLSL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12620
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.047"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            64..801
                     /gene="erm(B)"
                     /locus_tag="VXN30_001036"
     CDS             64..801
                     /gene="erm(B)"
                     /locus_tag="VXN30_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV1357673.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(1058..2305)
                     /locus_tag="VXN30_001037"
     CDS             complement(1058..2305)
                     /locus_tag="VXN30_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1357674.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            2485..2706
                     /locus_tag="VXN30_001038"
     CDS             2485..2706
                     /locus_tag="VXN30_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357675.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            2823..3320
                     /locus_tag="VXN30_001039"
     CDS             2823..3320
                     /locus_tag="VXN30_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEV1357676.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            3295..3801
                     /locus_tag="VXN30_001040"
     CDS             3295..3801
                     /locus_tag="VXN30_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV1357677.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            3785..6232
                     /locus_tag="VXN30_001041"
     CDS             3785..6232
                     /locus_tag="VXN30_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1357678.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            6235..8412
                     /locus_tag="VXN30_001042"
     CDS             6235..8412
                     /locus_tag="VXN30_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1357679.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            8409..9410
                     /locus_tag="VXN30_001043"
     CDS             8409..9410
                     /locus_tag="VXN30_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEV1357680.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            9407..10342
                     /locus_tag="VXN30_001044"
     CDS             9407..10342
                     /locus_tag="VXN30_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV1357681.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            10587..10703
                     /locus_tag="VXN30_001045"
     CDS             10587..10703
                     /locus_tag="VXN30_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV1357682.1"
                     /translation="MILKYPENICMLFMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            10719..>12620
                     /gene="tet(M)"
                     /locus_tag="VXN30_001046"
     CDS             10719..>12620
                     /gene="tet(M)"
                     /locus_tag="VXN30_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV1357683.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQKYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYM"
BASE COUNT         4044 a         2282 c         2598 g         3696 t
ORIGIN      
        1 ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt caggagagat
       61 tacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac
      121 caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt
      181 aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta
      241 gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta
      301 attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg
      361 aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga aagccgtgcg
      421 tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga
      481 acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg
      541 gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc
      601 acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat
      661 cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa
      721 gtaaacaatt taagtaccat tacttatgag caagtattgt ctatttttaa tagttatcta
      781 ttatttaacg ggaggaaata attctatgag tcgctttttt aaatttggaa agttacacgt
      841 tactaaaggg aatggagata aattattaga tatactactg acagcttcca agaagctaaa
      901 gaggtcccta gcgcctacgg ggtatttgta tcgataaggg gtacaaattc ccactaagcg
      961 ctcgggaccc cttgtaggaa aatgtcctaa gtgggatatc tgtcaactgg tatggttgac
     1021 taaaaatact tcctacgaaa atgtaggggg tattttttta cgaaaaaata caatcgattc
     1081 ttaaaaagaa aaatttttga ttggcaaaac cataacaagt tcgttttagg gttttgattt
     1141 tgcgattgat gccttctaaa ggaccattag agtattcaaa tttagcgcta tttaagacat
     1201 attttctgtt ttgacgaagg gtttgaatag cagtatccat ttctgtattg gttttttggt
     1261 agtctaagat ggttgactct agtaattcac tattgcgctc gtttagggct ttcgtgatat
     1321 cttggtaagt ttggtatact tcagcgaact tggaaaattt actagtaatg agatcaacag
     1381 cattttggcg agtcatatat tgtttaacgc cgcgaagaaa aactacttct tcagggtgaa
     1441 gatcttcagc ttttttatgg aatagcttcc aatgtgactt cataatttta tattcttggc
     1501 tctgtttatc aagttgcttt aggatagaga tacgacaatt gtccaaagcg cgaccagcta
     1561 attgtacaag gtggaagcga tcaataatga tattggcatt agggaaaagg cgatagataa
     1621 aactttgata ttgagcattt aaatcaatta caactgattg aacgcattcg cgttcggctt
     1681 ttgaataacg actttcaaaa taatcaacaa tggtaggtga tagacgatcc tgtaactttg
     1741 tgacaatttg gtgggtttca gcgtcacaac agataaagga catcacagac ttaattgaac
     1801 gaaactcgtc aaaacataga tgcttaggca acttagccac acgatagtgt ggttccatgc
     1861 gctctaagat tgttcgacga acactgctag gagagcagtg acacatttca gcaataagct
     1921 gaccagataa gcctttacga gctaaaagca tgatttgatt tttgagatca ctggataagg
     1981 tttgattttc tttggttaaa ttagtaatag caccaaaagt agtatggcat gatttacatt
     2041 tatagcgttg tttacgaagc tctagttcat atcttctccc atttaaactt gccagtcgta
     2101 catgagtttt gcgaaagcca tccttattaa ctgtgggaaa gccacagtta cgacaacgat
     2161 taatcggata agaaagagta gctgttatta gcgttatata ctctttaaca gaatcgttgt
     2221 tgtgttcagc ttcttcaaca gaaataattt taatattttt atctttaatt ccaagaatat
     2281 ttaggataga atcattatgg gacatttgtt taaccttctt tcatgatttt tgtggtgaat
     2341 tgattgtata acgaggggac agcaaatgtc ctcttttttg tataaaaaaa tctggcatgg
     2401 aatctctatc cataccagaa agtgtatacc ccaaaaaaat aactcaaata caaattcatt
     2461 gaatatagag aggagaacat ttttatgaat tttggacaaa acctttataa ctggtttcta
     2521 tcaaacgctc aatcactggt gcttttagca atcgttgtga ttggcttgta tcttggcttc
     2581 aagcgtgagt ttagcaaact gattggcttt ttaattattg cgattattgc ggttggctta
     2641 gtcttcaacg ctgctggagt aaaagacatt ttactagagc tattcaatcg cattattggt
     2701 gcttaaataa aaccgttctt ttgtggaata taagtggttt tcttatgttc cgcaaaggaa
     2761 tggtacacca aacgaagtgc ggtagggatt tttgaatctc tacaaagaaa ggacgtgaat
     2821 atatggacga tatgcaagtc tatattgcga atttaggcaa atacaatgag ggcgaattgg
     2881 tcggtgcgtg gtttaccttt cccattgact ttgaggaagt caaagagaaa atcggcttga
     2941 atgatgaata tgaggaatac gccattcatg actacgagtt accctttacg gttgacgaat
     3001 acacttccat tggcgaactc aatcgactat gggaaatggt atcggaatta cccgaagaat
     3061 tacaatcgga gctatctgct ctgctcactc atttttcaag cattgaagaa ctaagcgaac
     3121 atcaagagga tattatcatt cattccgatt gtgatgatat gtatgacgtg gcacgctact
     3181 acattgaaga aacgggtgct ttaggcgaag taccagctag tcttcaaaac tatattgatt
     3241 atcaagccta tggtcgggat ttagaccttt caggaacgtt tatctcaacc aatcatggga
     3301 tttttgaaat cgtctattaa atctgtcggt acattactac tggcagattt tctattttac
     3361 ggggtggctc aatcagctac ccctattttt tatgaaagga ttgattacat gaagaaaata
     3421 cgaagctata ccagtatctg gtctgtggaa aaggtactgt attctatcaa tgattttaga
     3481 cttccgtttc ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtg
     3541 atgatacttg gcaacttgcc ccctctttcc atgatagagg gagcatttct caaatacttt
     3601 gggattcctg tggctttcac atggtttatg tctacaaaaa cttttgatgg taaaaagcct
     3661 tatggatttt tgaagtctgt cattgcttat gcactgcgac caaagctgac ctatgcagga
     3721 aaaaaagtaa cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa
     3781 ttttatggca tatccaatta aatacattga aaacaatctc gtctggaata aagacgggga
     3841 atgttatgct tactatgagc ttgttcctta caattactca tttctaagtc cagaacagaa
     3901 aatacaagtg catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaaaattca
     3961 tgctttacaa atcagtacag aatccagcat acgttctgca caagagcgtt ccaaaaatga
     4021 agtcactggc aagctcaaag cggttgccta tgacaaaatc gaccaacaga cagacgcttt
     4081 aatatccatg attggcgaaa atcaagtgaa ctaccgtttc tttatcggct ttaagttgct
     4141 tctcaacgat caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgctttgtc
     4201 tgattttgtc tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga
     4261 tgaaatcctg cgttttcaga agatggaaaa gctcttagaa aataaaatct ctcgtcgttt
     4321 caaaatccgc aggttagata aggacgactt cggctatctg attgaacacc tttacggaca
     4381 gacaggcact gcctatgaag agtatgagta ccatctatca aagaaaaagc tggataatga
     4441 aacgctgatt aaatactatg acttgattaa gcctactcgc tgtttggtgg aagaaaaaca
     4501 gcgatatttg aaaatccagc aggaagatga aaccgtctat gtagcttact ttaccattaa
     4561 cagcattgtc ggagaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca
     4621 atttacattc ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct
     4681 atctactgtc cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag
     4741 tgataatgaa accagctcca atgtggcgga agctctggaa agtgtgaatg agctggaaac
     4801 caatttagac caaagcaagg aatctatgta caagctgtct tatgtggtaa gggtatcagc
     4861 aaatgatctt gacgaactca aacgtcgttg taatgaagtg aaagattttt atgacgattt
     4921 aagcgtaaaa ctggtacgac catttgggga tatgctcggc ttacatgaag aatttttacc
     4981 tgccagcaag cgttatatga atgattatat tcaatacgtg acctctgatt tcctcgctgg
     5041 tttaggtttt ggtgctactc aaatgctggg ggaaaatgag gggatttatg ttggctacag
     5101 cttagatact ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg
     5161 ttcagtaacc aatgcgttag cgtcggcttt tgttggttcg ctgggtggtg gtaaatcctt
     5221 tgcgaataac cttatcgtct attatgcggt gctttatggg gcacaagcag tgattgtaga
     5281 cccaaaagca gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa
     5341 tatcgtcact ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtgattat
     5401 gaaaaatccc aaagattctg aatcactggc tattgatatt ctgacattcc ttacggggat
     5461 ttcctctcgt gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa
     5521 tagtgaagta cgagggttga tgaaagtgat tgaggaatta cgggttgaga atacgccact
     5581 aagtaccagt atagccgacc atatcgaaag ttttacagac tatgactttg cacatttatt
     5641 attcagtaat ggttatgtgg agcagtctat cagcttagaa aaacaactga acattataca
     5701 ggttgcggac ttggtacttc ccgacaagga aacttccttt gaggaatata ccactatgga
     5761 gcttttatcc gttgctatgc tgattgtcat tagtaccttt gctttagact ttatccatac
     5821 agaccgaagc attttcaaga ttgtagattt agacgaagca tggagctttt tacaggtagc
     5881 acaaggaaaa acactatcta tgaagctggt tcgggctggt cgtgctatga acgctggggt
     5941 atatttcgtg acccaaaata cagacgacct cttagatgaa aaactgaaaa ataacctcgg
     6001 cttaaaattt gcatttcgtt ccactgacct taacgagatt aaaaagacct tagccttttt
     6061 tggtgtagac ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca
     6121 atgccttatc agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga
     6181 agaactgctc catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa
     6241 ccatcaatag taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca
     6301 atgacagtgg ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg
     6361 gttcaagctg cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac
     6421 ccacttgaaa actatcaact ggatttttat gtggataata gctggggctg gcttccgtgg
     6481 aactggtcgg acgggattgg aaaacaggtc atgtatggac tatatgccat taccaatttt
     6541 atttggacaa tcagtttgta tgtttccaat gcgacaggtt acttagtaca ggaagcctat
     6601 tccttagact tcatttccgc tacagcagat tccattggta agaatatgca gaccttagct
     6661 ggtgtgagtg caaacggatt ttcaacagag ggtttctatg ttggattcct cttactcttg
     6721 attttggttc ttggggttta tgttgcctat acgggactga taaagagaga aaccacaaag
     6781 gcaattcatg ccattatgaa ttttgtgctg gtgtttatcc tatcggcttc ctttattgcc
     6841 tacgctcccg actacattaa aaaaatcaat gacttttcat cagacatcag taatgccagt
     6901 ttatcacttg gcacgaagat tgtcatgccc cattccgata gtcaaggcaa ggacagcgtg
     6961 gacttaatca gagatagcct gttttccata caggttcagc aaccgtggct actgcttcaa
     7021 tacaacagtt cagacattga aagtatcggt attgaccgtg tggaaagcct gctctccacc
     7081 agcccagatt ccaacaatgg cgaagacaga gaaaaaattg ttgcggaaga aattgaagac
     7141 agaagcaata ccaatctaac cattacaaag accattaacc gtttaggtac agtcttcttc
     7201 ctatttgtct tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt
     7261 ttctcgcagg tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc
     7321 atgattccat catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt
     7381 ttgacacgag ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc
     7441 tataccttat cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt
     7501 gcaggaatct acttcaagct gggcgattta atgagtatgt tttctctaca gagtaacgat
     7561 tctcaaagtg tgggaagtcg tgtgatgaga aaacctcgta tgcttatgca cgctcacatg
     7621 caccgtctac agcggaaact tggacgttcc atgactactc taggggctgg gtctgccatt
     7681 gttacaggta aaaaaggaca gtcgggttcg gggagttctg caaggacaca agcagatcac
     7741 tcccgaccag acggaaagga aaaatcaaca cttggaaaac gtatcggtca aaccatcggt
     7801 acagtagctg ataccaaaga cagaatggta gacactgcta gtggtttgaa agaacaggtt
     7861 aaagatttgc cgaccaatgc aagatatgca gtatatcaag gaaaatccaa agtaaaagag
     7921 aatgtccgtg atttaaccag tagtatttct caaaccaaag cggacagagc cagtggacgc
     7981 aaggaacagc aggaacaaag gcgaaaaacc attgcgaagc gtcgctctga aatggaacag
     8041 gtcaaacaga aaaaacagcc tgcttcttct gttcatgaaa gaccgactac aagacaagaa
     8101 caatatcatg atgaacagac ctcaaaacag tctaatattc agacttcata taaggaatct
     8161 caacaagcca aacaagagcg tccagcagtt aagtccgatt tttcaagtcc aaaagtggaa
     8221 cgccaaggca ataccgttca agaaaaaacc gttcaaaagc cagcaacttc aaccactaca
     8281 gcagatagaa cttcacaacg tccaatcaca aaagaacgtc cgtctactgt tcaaagagta
     8341 ccactacaaa atacaagaag tagaccacca atcaaaaccg ccaccattaa gaaagtcggt
     8401 aagaaaccat gaagttgaaa actttagtga ttggtggttc tggattattc ttgatggtct
     8461 tctcactgct tctgtttgtt gccattttat tttcagatga acaggacagc ggaatttcca
     8521 atattcatta tggaggtgtg aatgtttccg cagaagtgct ggctcataag cctatggtag
     8581 aaaaatatgc caaagaatat ggcgttgaag aatatgtcaa catacttctt gcgattatac
     8641 aggtggaatc gggcggtact gcggaagatg ttatgcagtc ctcggaatcc ctcggtcttc
     8701 cacctaattc attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgaat
     8761 tattagccag tagcgaaagg ctcagtgtag atttagaatc ggttatccag tcctacaatt
     8821 atggtggtgg tttcttaggg tatgtggcta atcgtggaaa taaatatacc tttgaactgg
     8881 ctcaaagttt ctcaaaagag tattcaggtg gcgaaaaagt gtcttacccc aatcccatag
     8941 ccatacctat caatgggggc tggcgataca actatggcaa tatgttttat gtgcaactgg
     9001 taacgcagta tcttgtcaca acagagtttg atgatgatac ggtacaagcc atcatggacg
     9061 aagcactgaa atatgagggc tggcgatacg tttacggtgg agcttccccg actacttctt
     9121 ttgattgtag cggactgaca caatggacgt atggaaaagc tggaattaac ttaccacgaa
     9181 ccgcacaaca gcaatatgat gtgacccagc atatcccact atcggaagca caagctggcg
     9241 atttggtttt ctttcattct acctataacg ctggctctta tattactcat gttgggatat
     9301 accttggcaa taaccgtatg tttcatgcag gcgacccaat cggttatgcc gacttaacaa
     9361 gcccctactg gcaacagcat ttagtgggag caggacgaat caaacaatga gaaaggaaga
     9421 tttaatgatg aaatttagaa aaaatcagaa taaagaaaaa cagataccaa aggaaaagaa
     9481 acctcgtgtc tactataagg tcaatcctca taaaaaggtt gtgattgcct tgtgggtact
     9541 tttagggctt agtttcagct ttgcgatatt caagcacttt acagctatag atactcatac
     9601 tattcacgaa acaactatca tagaaaagga atacgttgat actcatcatg tagaaaattt
     9661 tgtagagaac tttgcgaaag tctactattc atgggagcaa tccgataagt ccattgataa
     9721 tcgaatggaa agtctaaaag gctatctgac agatgaactt caagctctca atgttgatac
     9781 agtacgcaaa gatattcctg tatcgtcttc tgtaagagga tttcagatat ggacggtaga
     9841 gccaactggc gacaatgagt ttaatgtaac ctacagtgta gaccagctca ttacagaggg
     9901 agaaaataca aagaccgtcc actctgctta tatagtgagt gtctatgtag atggttctgg
     9961 aaatatggta ctggttaaga atccgaccat taccaacata cctaagaaat caagttataa
    10021 accaaaagcc attgaaagtg aggggacggt tgattccatt acaaccaatg aaatcaatga
    10081 gtttttaacg acgttcttca agctctatcc tacagcgaca gccagtgaac tttcctacta
    10141 tgtgaatgac gggatattaa aaccaatcgg aaaagagtac atctttcaag aactggtaaa
    10201 tcctattcac aatcgtaagg ataatcaagt cacggtatcg ctgacagtgg agtatatcga
    10261 ccagcagacc aaagcaacgc aggtatctca atttgatttg gtacttgaaa agaacgggag
    10321 taattggaag attatagaat aacaaatatt ggtacattat tacagctatt ttgtaatcac
    10381 gtactctctt tgataaaaaa ttggagattc ctttacaaat atgctcttac gtgctatcat
    10441 taaagtatct atttaaaagg agttaataaa tatgcggcaa ggtattctta aataaactgt
    10501 caatttgata gtggaaacaa ataattggat gtcctttttt aggagggctt agttttttgt
    10561 acccagttta agaatacctt tatcatgtga ttctaaagta tccggagaat atctgtatgc
    10621 tttttatgcc tatggttatg cataaaaatc ccagtgataa gagtatttat cactgggatt
    10681 tttatgccct tttgggcttt tgaatggagg aaaatcacat gaaaattatt aatattggag
    10741 ttttagctca tgttgatgcg ggaaaaacta ccttaacaga aagcttatta tataacagtg
    10801 gagcgattac agaattagga agcgtggaca gaggtacaac gaaaacggat aatacgcttt
    10861 tagaacgtca gagaggaatt acaattcaga cggcgataac ctcttttcag tggaaaaata
    10921 ctaagataaa catcatagac acgccaggac atatggattt tttagcagaa gtatatcgtt
    10981 cattatcagt attagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac
    11041 aaactcgtat attgtttcat gcacttagga aaataggtat tcccacaatc ttttttatca
    11101 ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac
    11161 tttctgcgga aattgtaatc aaacagaagg tagaactgca tcctaatatg cgtgtaatga
    11221 actttaccga atctgaacaa tgggatatgg taatagaagg aaatgattac cttttggaga
    11281 aatatacgtc tgggaaatta ttggaagcat tagaactcga acaagaggaa agcataagat
    11341 ttcataattg ttccctgttc cctgtttatc acggaagtgc aaaaaacaat atagggattg
    11401 ataaccttat agaagtgatt acgaataaat tttattcatc aacacatcga ggtcagtctg
    11461 aactttgcgg aaaagttttc aaaattgagt attcggaaaa aagacagcgt cttgcatata
    11521 tacgtcttta tagtggcgta ctgcatttgc gagattcggt tagaatatcg gaaaaggaaa
    11581 aaataaaaat tacagaaatg tatacttcaa taaatggtga attatgtaaa atcgataagg
    11641 cttattccgg ggaaattgtt attttgcaga atgagttttt gaagttaaat agtgttcttg
    11701 gagatacaaa gctattgcca cagagagaga gaattgaaaa tcccctccct ctgctgcaaa
    11761 cgactgttga accgagcaaa cctcaacaaa gggaaatgtt acttgatgca cttttagaaa
    11821 tctccgacag tgacccgctt ctgcgatatt atgtggattc tgcgacacat gaaatcatac
    11881 tttctttctt agggaaagta caaatggaag tgacttgtgc tctgctgcaa gaaaagtatc
    11941 atgtggagat agaaataaaa gagcctacag tcatttatat ggaaagaccg ttaaaaaaag
    12001 cagagtatac cattcacatc gaagttccac cgaatccttt ctgggcttcc attggtctat
    12061 ctgtagcacc gcttccatta gggagcggag tacagtatga gagctcggtt tctcttggat
    12121 acttaaatca atcgtttcaa aatgcagtta tggaggggat acgctatggc tgtgaacaag
    12181 gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc ttatactata
    12241 gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg gaacaagtct
    12301 taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt tatgcgccac
    12361 aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca
    12421 ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg tgtattcaaa
    12481 aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta acagagttaa
    12541 aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga
    12601 tagataaagt acgatatatg
//
LOCUS       DAVLSL010000047        12614 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLSL010000047 DAVLSL010000000
VERSION     DAVLSL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.048"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(21..956)
                     /locus_tag="VXN30_001047"
     CDS             complement(21..956)
                     /locus_tag="VXN30_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1357684.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(953..1812)
                     /locus_tag="VXN30_001048"
                     /pseudo
     CDS             complement(953..1812)
                     /locus_tag="VXN30_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            1920..4082
                     /locus_tag="VXN30_001049"
     CDS             1920..4082
                     /locus_tag="VXN30_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1357685.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4174..5433
                     /locus_tag="VXN30_001050"
     CDS             4174..5433
                     /locus_tag="VXN30_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1357686.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTSFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5447..6313
                     /locus_tag="VXN30_001051"
     CDS             5447..6313
                     /locus_tag="VXN30_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1357687.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6327..7160
                     /locus_tag="VXN30_001052"
     CDS             6327..7160
                     /locus_tag="VXN30_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1357688.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7161..7241
                     /locus_tag="VXN30_001053"
                     /pseudo
     CDS             7161..7241
                     /locus_tag="VXN30_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7518..8960
                     /gene="gtfA"
                     /locus_tag="VXN30_001054"
     CDS             7518..8960
                     /gene="gtfA"
                     /locus_tag="VXN30_001054"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1357689.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9098..10291
                     /locus_tag="VXN30_001055"
     CDS             9098..10291
                     /locus_tag="VXN30_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1357690.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10756..12102
                     /locus_tag="VXN30_001056"
     CDS             10756..12102
                     /locus_tag="VXN30_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV1357691.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     LISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDIISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFHHLFLH
                     VVGNCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
BASE COUNT         3695 a         2256 c         2641 g         4022 t
ORIGIN      
        1 tatattataa ttgtgttatt ttacttccaa ctattgagag aaatttcgcc actatttagc
       61 cagatttctt ttccgttatc acattgaact aaaagttttc cattttctga gatgtcttta
      121 gcaagtccct tgtagtcttt ttgctgtagt gtgaaagtga cttcttttcc tagaatgaat
      181 gactgttttt tgtataggta taatagctct tctgctggtg tttcgaagaa agcacgccag
      241 atttctatga tcaattcatt ccttgttata ggagctgtag ctttaaataa gctggcagct
      301 ttttctttta attcctgagg gaagtcttta atagtgaaat tgatacctac tccaataatg
      361 atatctgtga ctaagccagt ttctacagag gtcattgctt cagtaaggat tcctccaatt
      421 ttatgattgt ttagatagat atcattgacc cattttatgt cgacatctat taaagttagg
      481 ttcttaatag ctttgtagac agctccagct acaagtagtg tgtaggatgg taatttgtca
      541 taggggagat ttggtttaag atggagtgtc atataaatac caccttgtgg tgagtagaag
      601 gaacgttgaa aacgtcctcg gcctgctgtt tgataggaag ctagatagag ggtatttgct
      661 tcatggccta aatcaattgc ttcttttgca tctagttgtg ttgattttgt ttcgggttta
      721 aagctgactt taattggaag attttcttct agaatctctg gaagaataag gtcaccattc
      781 attagtttat atcctctatt tttgatacta tcaatttcaa tgccttcttg ttctagtcgc
      841 ttgatagctt tccaaattga tgttcggctt agggatagtt tttctgcgat tttttctccg
      901 ctgatatagt cggtttcttt agataggatt tggtagacag cttggtagga tttcataatg
      961 ttgcctttct cactagttgg tattgagagt attcttttct tgtatgactt ggagtctgat
     1021 taaagtattg tttataagct ttcgaaaaat ggagtggatc tgaaaaacct accgagtatg
     1081 caattacctt gatggactct tgggtatttt cgagaagttg tctagctcgg tgcattcgaa
     1141 cgtagagtag gtattctttg ggtgataagg tattaaattc tttgaatacg cttgataagt
     1201 agcttctgtg aacggatagt tcttttgcta aatcttgaat tgtaagtgat tgaggatagt
     1261 ggctatcaat taatcgtttg cattcaagat agagttggtg ggttgatgaa atattctttt
     1321 tttctgattg ggagcaatag ttcccagatg aaacatcagt tcatgaagtt gtcccatgat
     1381 atggagttga gctaattcac ttgattttgt aatctgagcg aagcggacaa tgtctgagat
     1441 gagttttgca gtagtctggg tatgacaagt ttcagattgg atgagatagg attgatcaga
     1501 aatttgagaa agagcaaaat aatcagggga tttccctcca gtgattccta accagtagta
     1561 ggcccaaggt tctttactat ctgcttgata aaaggttagt tcctctggtt ttaatagaaa
     1621 gaaatctcct tcttttaaat caacaatttt atccttgtaa tgaaattttc cttgtccttt
     1681 agtaatgtaa tgtaggacgt atgtatcacg aatggctgga ccaaaagagt aattaggtgt
     1741 gcattcctca tatccataaa agcttagggc aaggtcgatt gttccagtct ggtattctga
     1801 aaaaactaac atgtgctacc tcctacctaa cattttacca tattcttgag acatttttct
     1861 attttggaaa gcgatttcag gtgataaaat ataatcaata aagtgatgag gtgaagtaaa
     1921 tgggagttag gatagagaat aatctatttt atgttgagag taaaaatcta agtttgatta
     1981 ttgaaaatcg aaatggctac ttacttttga aacatttagg aaagactatt aagaactata
     2041 aaggttccaa tagtgtttat gaacgagacc atgccttttc aggaaatcca acggctacta
     2101 atcgaacctt tagtttagat actcagcgac agatttttgg acaacatggc ttaggagatt
     2161 ttaggaaacc aactatacag gttcagcata gtgtaactga agtaacagac tttcgatttg
     2221 tagaagcaaa gattttaaaa ggtcagaatg gtccacaggg cttaccttct ccacatagca
     2281 tggacgatac agagactctt gtcttaatgt tagaagattc taaggctcaa cttagtctga
     2341 ctttgtatta tactactttt aataatgatg cgactattac tagctacagt aaattagata
     2401 ataatagtaa tcaggaagtt gtcatccata aagatttttc ttttatggct gattttccag
     2461 ctgcagatta tgaaatagca actctgcagg gtgcttatgc tcgtgaaaag actgttagac
     2521 gtcaacaggt agaacaagga atcttttcga ttagttcaaa ccgaggtgct tctggtcatg
     2581 ctcaaacacc agctcttcta ctatgcgaac aaggagtcac agaggatgct gggaatgtgt
     2641 ttgctattca actaatgtat agtggcaact ttgaagcttt tgttcaaaaa aatcaattga
     2701 atgaagttcg ggtggctatt ggcattaatc cagaaaactt ttcttggaag ttagcccctg
     2761 aggaatactt tgaaacaccg gtagctttag tgactcattc agatcaggga ttaactggta
     2821 ttagtcatga aagtcagaat tttgtactga agcacattat gctaagtgaa ttttctaaaa
     2881 aagaacgtcc aattctaatc aataactggg aagctactta ctttgacttt cagagagaaa
     2941 aactgttaga gttagcagat gaagctaaga aagttggcat tgaacttttt gtattagatg
     3001 atggttggtt tggcaatcgt tttgatgata atcgtgcttt aggtgattgg gttgttaatg
     3061 aggaaaaact gggtggaagt ctagaaagtc tgatttcagc tatccatgaa agaggtttgc
     3121 agtttggact ttggttagaa cccgaaatga tttctgtaga tagtgatttg tatcgtcaac
     3181 atcctgactg ggctattcag gttcctgatt atgagcatac ttattctcgg aatcaattag
     3241 tacttaatct tgccaatcct caggtagtag aatacttgaa aagtgtctta gatcaactcc
     3301 tatcttatca tgagattgat tacattaaat gggatatgaa ccgcaatatc actaagctag
     3361 ggaatggatt aacttatcta gagacacaga tgcaatctca tcagtacatg ctggggcttt
     3421 acgaactcgt ttcttatctg acagagaagc acagccatat tctctttgag tcctgctctg
     3481 gtggtggtgg acgaaatgat cttggtatga tacgctattt cccacaagtc tgggctagtg
     3541 ataatactga tgccattgca cgtttaccaa ttcaatacgg ttcatcctat ctctatccaa
     3601 ccatttctat gggggctcat gtgtcagcag taccgaatca tcagatggga cgaatgacac
     3661 cattagaaac acgtggccat gtagcaatga tgggaaattt gggctatgag cttgatttga
     3721 caaatttatc agatgaagag aaagctacga ttgctaatca ggtgaacttg tataaagaat
     3781 tacgaccagt agttcagtta ggacagcagt atagactaat taatcctgat actgcatcca
     3841 atgaagcagc tgtacaattt aattacggaa atcaaacgat tgtaacctac gttcgcgttt
     3901 tatctgttgt agagaccatg gaaacaactt taaaattaaa agatttggat gaagagggac
     3961 tatataaatt acaggaaaat ggcgaagttt actcaggtgc agaactaatg tatgcgggct
     4021 taactgttat cttatcccaa ggagattttt tgagtagaca gtatattttt agaaaactat
     4081 gatatgtact gaccccaaaa gttagattta ttctgtctaa cttttggggt gcagttcaat
     4141 aagggtgtat aaatttataa aggaggcttt ctaatggaat ggtataaaaa aatcggactt
     4201 cttgcaacta caggtttagc tttgtttggg ctcggcgctt gctccaacta tggtaaatct
     4261 gcggatggca cagtgaccat cgagtatttc aaccagaaaa aagaaatgac caaaaccttg
     4321 gaagaaatca ctcgtgattt tgagaaggaa aaccctaaga tcaaggtcaa agtcgtcaat
     4381 gtaccaaatg ctggtgaagt attgaagaca cgcgttctcg caggagatgt gcctgatgtg
     4441 gtcaatattt acccacagtc catcgaactg caagaatggg caaaagcagg tgtttttgaa
     4501 gatttgagca acaaagacta cctgaaacgc gtgaaaaatg cctacgctga aaaatatgct
     4561 gtaaacgaaa aagtttataa cgttcctttt acagctaatg cttatggaat ttactacaac
     4621 aaagataaat tcgaagaact gggcttgaag gttcctgaaa cctgggatga atttgaacag
     4681 ttagtcaaag atatcgttgc taaaggacaa acatcatttg gaattgcagg tgcagatgct
     4741 tggacactca atggttacaa tcaattagcc tttgcgacag caacaggtgg aggaaaagaa
     4801 gcaaatcaat accttcgtta ttctcaacca aatgccatta aattgtcgga tccgattatg
     4861 aaagatgata tcaaggtcat ggacatcctt cgcatcaatg gctctaagca aaagaactgg
     4921 gaaggtgctg gctataccga tgttatcgga gccttcgcac gtggggatgt cctcatgaca
     4981 ccaaatgggt cttgggcgat cacagcgatt aatgaacaaa aaccgaactt taagattgga
     5041 accttcatga ttccaggaaa agaaaaagga caaagcttaa ccgttggtgc gggagacttg
     5101 gcatggtcta tctcagccac caccaaacat ccaaaagaag ccaatgcctt tgtggaatat
     5161 atgacccgtc cagaagtcat gcaaaaatac tacgatgtgg acggatctcc aacagcgatc
     5221 gaaggggtca aacaagcagg agaagattca ccgcttgctg gtatgaccga atatgccttt
     5281 acggatcgtc acttggtctg gttgcaacaa tactggacca gtgaagcaga cttccatacc
     5341 ttgaccatga actatgtctt gaccggtgat aaacaaggca tggtcaatga tttgaatgcc
     5401 ttctttaacc cgatgaaagc ggatgtggat taggagtaga gagactatga aaaaagtatt
     5461 acaaaaatat tgggcatggg cttttgtggt catccccctc ttgttacaag caattttctt
     5521 ctatgtgccg atgtttcaag gagcctttta cagttttacc aactggacag gattgactta
     5581 caactacaaa tttgtcggct taaacaactt taagctcctc ttcatggatc caaaattcat
     5641 gaatgcgatt ggctttaccg caatcattac gattgccatg gtggttggtg agattgcact
     5701 cgggatcttc attgcgcgtg tcttgaactc taaaatcaaa ggccaaacct tcttccgtgc
     5761 ttggttcttc ttcccagctg ttttatctgg tttgacagtg gctttgatct tcaagcaagt
     5821 cttcaactac ggtcttccag cgattgggaa tgcccttcat attgaatttc tccaaaccag
     5881 tcttttaggg actaagtggg gagcaatctt tgcggctgtc tttgtccttc tttggcaagg
     5941 ggtggctatg cccatcatca tcttcctagc tggtttgcaa tctattccaa ctgagattac
     6001 agaggcagca aggattgatg gtgcgactag caagcaagtt ttctggaaca ttgaattgcc
     6061 ttacttgcta ccaagtgtct ctatggtctt tatcctagcc ctaaaaggtg ggctgactgc
     6121 ctttgaccaa gtctttgcca tgaccggtgg tggtccaaac aatgccacaa cctcacttgg
     6181 gctcttggtt tataactatg cctttaaaaa caaccaattc ggttatgcca atgccattgc
     6241 cgtaatcttg ttcctcttaa ttgtagtgat ttcgatcatc caattgagag tatctaagaa
     6301 atttgaaatt taagaggaga agcatgatga aacaagatga aagaaaagcc ctgattggca
     6361 aatacattct attgattcta ggatcggttc tgattttagt gccgctcctt gctaccctct
     6421 ttagttcctt caaacccact aaggatattg tagataattt ctttggcttt ccaaccaact
     6481 tcacatggga caactttagc cgtctcttag ctgatgggat tggaggctat tattggaact
     6541 ctgtcgtcat cactgtcttg tctttacttg cagtaatgat ctttatccct atggcagcct
     6601 actccatcgc tcgcaatatg agtaaaagaa aagtctttac catcatgtat accctcttaa
     6661 tcctcggaat cttcgtacct ttccaagtca tcatgattcc gattacggtt atgatgagta
     6721 aactcggttt ggctaatacc tttggtttga tcttgctcta cttgacctat gcgattccac
     6781 agaccctctt tctctatgtt ggctatatca aaatctcgat tccagaaagt ctggatgaag
     6841 cagcagagat cgatggggct aatcaattta caacctattt ccgcatcatc ttcccaatga
     6901 tgaaaccgat gcatgcgaca accatgatca tcaatgccct ttggttctgg aatgacttca
     6961 tgttgccact ccttgtcttg aaccgggatt ccaaaatgtg gactctgcct ttgttccaat
     7021 acaactacgc aggccaatat ttcaacgact acggaccaag ctttgcctct tacgtggtcg
     7081 gcattatcag tatcaccatt gtctatctct tcttccaacg ccatatcatt tcaggaatga
     7141 gcaacggggc agtgaagtaa tactcaatga aaatcaaaga gcaaactagg aagctagccg
     7201 caggttgctc aaaacactgt tttgaggttg tggatgaaac tgacgaagtc agtaaccata
     7261 cctacggtaa ggtgaagctg acgtggtttg aagagatttt cgaagagtat aagaagagct
     7321 catggaatct atagtctttg acacagagga agagttcagc ttgttgataa ttctatgtag
     7381 aaaacaagaa agtagtactt tgtttatcac agtctatatt agtacgagtt tgatgataaa
     7441 tagagattta aaatgaattt gatttacgaa gccagatcat atgagacttg gcttcaatta
     7501 gaaaaaggag agtaatgatg ccgattcaaa ataaaaccat gttgattacc tattctgata
     7561 gccttggaaa taatcttaaa gacttatatg ataatttgga agagcatttt ggagatgcta
     7621 ttggaggagt tcacctttta ccatttttcc catcaacagg tgatcgtgga tttgcgccag
     7681 ttgactacga cgaagtggac tcagcttttg gtgattggga ggatgttaag cgtttaggtg
     7741 agaaatatta tcttatgttt gattttatga ttaatcatat ttctcgtcaa tccaagtatt
     7801 ataaggacta tcaagaaaaa catgaagcca gtgaatttaa agctctcttt ttaaactggg
     7861 ataagttttg gccagaaaac cgtccgacac agtctgatgt agatttaatt tacaagcgta
     7921 aggatcgtgc accaaagcaa gagattgtgt ttgaagatgg ttcagtggaa catttgtgga
     7981 atacctttgg tgaggagcag attgatcttg atgtgaccaa agaagtaact atggaattta
     8041 tccgtaagac cattcagcac ttggcaagta atgggtgtga tttgattcgt ctagatgcct
     8101 ttgcttatgc agtgaagaaa ttggatacta atgatttctt tgtggaacca gatatttggg
     8161 atttattgga caaagttcga gatatcgctg ctgagtatgg gacagagctt ttacctgaga
     8221 ttcatgaaca ctattcgatt cagtttaaaa tagcagacca tgattactat gtttatgatt
     8281 ttgctcttcc aatggtgaca ctttatactc tttacagttc cagaacagag cgtttggcta
     8341 agtggttaaa gatgagcccg atgaagcaat ttacgacgct agatacccat gatgggattg
     8401 gagtagtaga tgtcaaggat atcctgaccg atgaggagat tgactatgct tcaaatgaac
     8461 tctataaggt tggagccaat gtcaaacgta agtactctag tgccgagtat aacaacttag
     8521 atatctacca aatcaattca acctactatt cagcgcttgg agatgatgat gtcaagtatt
     8581 ttctcgctcg tctaattcaa gcttttgccc caggtattcc tcaggtttac tatgtgggtc
     8641 tattagcagg caagaatgac ttgaaattat tagaagaaac taaagaaggt cgaaatatta
     8701 atcgtcatta ctatagcaac gaggaaatag caaaagaagt gcaacgacct gttgtgaagg
     8761 cccttctcaa tctattttct ttccgtaacc gttcagaagc ctttgatcta gaagggacta
     8821 ctgagataga gacaccaaca gctcacagca ttgtaatcaa acgtcaaaat aaagataagt
     8881 ccgtaacagc agtagcagaa attgacttgc aaaatcagac ttatcgggta attgagaatg
     8941 gagttgaagt aacattttga agccttgata tggtggataa aatagggttt ttattttgga
     9001 aaacgtttcc tttgttttca aattgctaaa aaagtggtac aatataggat agcttactat
     9061 tatctgaatc agctgatttg gagagaaagg attcattttg aaatcaatag gctttattga
     9121 aaagctgaag gggttgtcta gtaaagagct gattttattg ggaattatcc tgagtatctt
     9181 tttacccttt tatctttttg tagttgtatt ctgtttatat attatcagtc tgatttttac
     9241 aggagacatg aaaagtattc ttcagaaaat gggggagcat tcgatgctgc ttctttttct
     9301 tggctatagt actgttatat ccattcttgc acaaaattgg atgggtcttg tggcttcagt
     9361 aggaatgttt ctatttacta ttttcttttt gcactatcag tcgattttat cccataaatt
     9421 ctttcgattg attttgcagt tcgtcttgtt tggtagtgtc ttgtcagctg cttttgccag
     9481 tttagaacat ttccaaattg tgaagaaatt taattatgct tttctttccc ccaatatgca
     9541 ggtgtggcat cagaaccggg cagaagtgac cttctttaat cctaattatt atggaattat
     9601 ttgttgtttc tgtattatga ttgctttcta tctgtttaca acgaccaagt tgaattggtt
     9661 gaaagtattc tgtgtgattg caggctttgt taatctcttt ggtttgaact ttactcaaaa
     9721 tcgaactgcc tttcctgcta ttatcgctgg agcaattatc tatctcttta cgactattaa
     9781 aaactggaag gccttttggc ttagtattgg ggtcttcgcg attggtttga gtttcctctt
     9841 ttctagtgat ttgggagttc gaatgggtac tttagactct tctatggaag aacgcatttc
     9901 tatctgggat gctgggatgg ccttgtttaa gcaaaatcct ttttggggtg aagggccatt
     9961 gacctatatg cactcttatc ctcggataca tgctccttat catgaacatg cccacagtct
    10021 ttatattgat acgattctga gttacggaat tgtgggtacc attttattag ttctgtcttc
    10081 tgttgctcct gttcgcttga tgatggatat gagtcaggag tcggggaaac gtccgattat
    10141 cggcctttat ctatctttcc ttacagtggt tgctgtgcac ggaatttttg acttggctct
    10201 cttctggatt cagtcaggct ttattttctt gctagttatg tgcagtattc cattggagca
    10261 tcgaatgttg gtatcggaca tgacggatta agttttataa gtttgaaatc ttctaccttg
    10321 ggtaggagat tttttatacg atgggcatgt cgtgcatctg aggtgtaagt cctctgtagg
    10381 gatccactac cggtgaatac aatagcgctc cccaagcctg actatcgtga ggtagcggat
    10441 taaaatggtc tggggataga tcgttttaag tctgacgcag gaaataagaa ttgtcagaga
    10501 aagcaatagt gaaatcgtag gctatgaata gaaacgtgat agcgaggagc atatagtgga
    10561 taaggtagcg agaaatttta aagtataaaa ccccaattat tcacccctaa tttaaaaacc
    10621 atccagaatc cttgccttag cttagatcct ggatggtttc ttttttcacc caatgggtgt
    10681 tttttactag acaaaaaaga gtttcccctt tatggtataa gtgtagaaaa aaacacaaaa
    10741 agaaaggaaa ctcacatgaa cagtttacca aatcatcact tccaaaacaa gtctttttac
    10801 caactatctt tcgatggagg tcatttaacc cagtatggtg gtcttatctt ttttcaggaa
    10861 cttttttccc agttgaaact aaaagagctg atttctaagt atttagtaac gaatgaccaa
    10921 cgccgctact gtcgttattc ggattcagat atccttgtcc agttcctctt tcaactgtta
    10981 acaggctatg gaacggacta tgcttgtaaa gaattgtcag ctgatgccta ctttccaaaa
    11041 ttgttggaag gagggcagct tgcttcacag ccaaccttat cccgttttct ttccagaact
    11101 gacgaggaaa cagtccatag tttgcgatgc ctcaaccttg aattggtcga attcttttta
    11161 cagtttcacc agctaaacca actcattgta gatatcgatt ctacccattt cacaacttat
    11221 ggcaagcaag aaggtgttgc ttataacgcc cactatcgtg ctcatggcta tcatcctctt
    11281 tatgctttcg aggggaagac aggttattgt ttcaatgccc agcttcgtcc tggtaatcgt
    11341 tattgttctg aagaggcaga cagctttatc acacctgttt tagaacggtt taatcaactt
    11401 ctctttcgaa tggatagtgg ctttgcgacc ccaaaattat acgatttaat tgaaaaaaca
    11461 gggcaatact acctcataaa actcaagaaa aatactgttc tgagccgtct tggagacctt
    11521 tccctccctt gcccacagga tgaggactta accatcttgc cccactccgc ctactcagaa
    11581 actctctatc aagcaggatc ttggtcgcac aagcgtcgtg tctgccagtt ctctgaacga
    11641 aaagaaggaa acttgttcta cgatattatt tctctcgtta caaatatgac gagtggaaca
    11701 agccaagacc agtttcagct ttatcgtgga cgtggtcaag ccgagaattt catcaaggag
    11761 atgaaggagg gattttttgg cgataaaacg gatagttcaa ccttaatcaa aaacgaagtt
    11821 cgtatgatga tgagctgtat cgcctacaat ctctatcttt ttctcaaaca tctagctgga
    11881 ggtgacttcc aaactttaac aatcaaacgc ttccaccatc tttttcttca cgtggtagga
    11941 aactgtgttc gaacaggacg caagcagctc ctcaaattgt ctagtctcta tgcctattcc
    12001 gaattgtttt cagcacttta ttctaggatt agaaaagtca acctgaatct tcctgttcct
    12061 tatgaaccac ctggaagaaa agcgtcgtta atgatgcatt aaagaacagt cgagatgaaa
    12121 aaatcgtgtg acgcaccaag ggaggagtct gcccttttga ggaaatctag cgaggaaaaa
    12181 cgatactgga acagcagaaa gtaaaactga cctcatgagg aggaagaaag tggctcatga
    12241 ggtcaggggt tttgtaagtt acatctagtt gagagaggta tgaatgattt gggtaaaaga
    12301 tagtcttaat ctatatgtgt aaatcacgaa agtaattgaa ttcggaataa ggttggtgtg
    12361 aaaagacata gtaaactgaa tcttgaatac tagaatgata cttctacaat attgttataa
    12421 tttaatttga attccaaaat cacattgttc cgtccgtatt tcattttact gtatgtcttc
    12481 ctaagctcca aagtttctgc gtcaaatttc ctattttcac tgtagtcttt gacacttcga
    12541 acgacaattt gaatcatata ttcaaaaaaa atgaaaaatt tgtgccaaca agcctaaata
    12601 ttctgaaaat ttgt
//
LOCUS       DAVLSL010000048        12324 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLSL010000048 DAVLSL010000000
VERSION     DAVLSL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.049"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            111..3212
                     /locus_tag="VXN30_001057"
     CDS             111..3212
                     /locus_tag="VXN30_001057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1357692.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPFYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3295..4302
                     /gene="pfkA"
                     /locus_tag="VXN30_001058"
     CDS             3295..4302
                     /gene="pfkA"
                     /locus_tag="VXN30_001058"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1357693.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4361..5866
                     /gene="pyk"
                     /locus_tag="VXN30_001059"
     CDS             4361..5866
                     /gene="pyk"
                     /locus_tag="VXN30_001059"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1357694.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDEVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6600..6825)
                     /locus_tag="VXN30_001060"
                     /pseudo
     CDS             complement(6600..6825)
                     /locus_tag="VXN30_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7673..8545
                     /locus_tag="VXN30_001061"
     CDS             7673..8545
                     /locus_tag="VXN30_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1357695.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8691..9035)
                     /locus_tag="VXN30_001062"
                     /pseudo
     CDS             complement(8691..9035)
                     /locus_tag="VXN30_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9178..9490
                     /locus_tag="VXN30_001063"
                     /pseudo
     CDS             9178..9490
                     /locus_tag="VXN30_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9494..10210
                     /locus_tag="VXN30_001064"
                     /pseudo
     CDS             9494..10210
                     /locus_tag="VXN30_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(10340..11155)
                     /locus_tag="VXN30_001065"
     CDS             complement(10340..11155)
                     /locus_tag="VXN30_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1357696.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTNLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11152..12057)
                     /locus_tag="VXN30_001066"
     CDS             complement(11152..12057)
                     /locus_tag="VXN30_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1357697.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
BASE COUNT         3858 a         2150 c         2615 g         3701 t
ORIGIN      
        1 tacaaatgaa tagaaaataa attagtaagc tgatgaaatt tttctcaaga gaagccattt
       61 ataggtgaaa atggtataat atagtgagaa ggatagagga gaagtgtaaa ttgatcgcac
      121 aactagatac aaaaacagtc tatagtttta tggaaagcgt catttcgatc gaaaagtatg
      181 tgagagcagc taaagaatac ggctacactc atttggctat gatggatatt gacaatcttt
      241 atggcgcttt cgactttcta gagattacaa aaaaatacgg cattcatcct ttgctagggc
      301 ttgaaatgac agtgtttgta gatgatcagg gagtgaattt gcgcttttta gctctatcta
      361 gtgtgggcta tcagcagttg atgaagcttt cgacagccaa gatgcagggg gagaaaactt
      421 ggtcagtcct gtcccagtac ctggaggata tcgcggtcat tgtgccttat tttgatagag
      481 ttgagtcgtt agaactaggc tgtgattact atataggggt ttatccagaa acactagcaa
      541 gcgaatttca tcatcctatc ttaccttttt atcgggtcaa cgcttttgaa agcagggata
      601 gagaagttct tcaagtttta acagcgatta aagaaaatct accgctcaga gaagttccct
      661 tgcgttcgag acaagatgtc tttatatcag caagttcttt agagaaacta ttccaagagt
      721 gttttccgca agctttggac aatttagaaa agcttatttc aggcatttct tacgacttgg
      781 atactagtct gaaactgcct cgttttaatc cagctagacc agcagtagag gagttgagag
      841 agcgtgctga actggggctt gttcagaagg ggttgactag taaagaatat caagatagac
      901 tagaccaaga attgtctgtt attcatgata tgggctttga tgattatttc ttggttgttt
      961 gggatttgtt gcgttttgga cgatcgaatg gctattatat gggaatggga aggggttctg
     1021 cagtaggcag tttggtttct tatgccttag acatcacggg gattgaccca gtagagaaaa
     1081 atctgatttt tgaacgcttt cttaatcgtg aacgctatac catgcctgat attgatattg
     1141 atatcccaga tatttatcgt ccagatttta tcagatatgt tggtaataaa tatggtagta
     1201 aacatgcggc acaaatcgtt actttttcaa cctttggagc caagcaagct cttcgagatg
     1261 tcttgaaacg ctttggtgtg ccagagtatg aattatctgc aattactaag aaaatcagtt
     1321 ttcgtgacaa tcttaagtcg gcctatgagg gcaatctcca gtttcgtcag caaatcaata
     1381 gtaagttaga ataccaaaaa gcttttgaga ttgcttgcaa gatagagggc tatccaagac
     1441 aaacctctgt ccatgcggct ggtgttgtaa ttagtgacca agatttaacc aactatattc
     1501 ctctaaagta tggtgatgaa attccactga ctcagtatga tgctcatgga gttgaggcta
     1561 gcggactttt gaagatggac tttctgggac tacgaaattt gacctttgtc cagaagatgc
     1621 aagagttgct tgctgaaata gaaggtattc accttaaaat tgaagaaata gatttggaag
     1681 acaaagaaac gttagcttta tttgcctctg gaaatacaaa aggtatcttt caatttgagc
     1741 aaccaggtgc cattcgtctg cttaagcgtg tgcaaccagt ctgttttgaa gatgtcgtcg
     1801 cgactacttc tctaaatcga ccgggtgcta gtgactatat caataatttt gtggcaagaa
     1861 agcatgggca ggaagaagtg actgttctgg atccagtact ggaggatatt ttggctccaa
     1921 cctacggcat aatgctctat caggagcagg ttatgcaggt tgcccagcga tttgccggat
     1981 ttagtcttgg gaaagccgat attttgcgtc gggctatggg gaaaaaggat gcctctgcca
     2041 tgcatgagat gagggcttcc tttattcaag gttcattaga agctggtcat actgtggaaa
     2101 aagcagagca ggtctttgat gttatggaga agtttgcagg ttatggtttt aacaggtcac
     2161 acgcctatgc ctactcagcc ttggccttcc agttggctta ttttaaaaca cattatccag
     2221 ccatttttta tcaggtcatg ttaaattctt ccaacagtga ttacttaata gatgcacttg
     2281 aagcaggttt tgaagtagcc tctctatcca tcaacaccat tccctatcac gataaaattg
     2341 ccaacaaggc catctatcta ggtttgaaat ccattaaagg agtcagtaat gatttagctc
     2401 tctggattat tgaaaataga ccttattcta acattgaaga ttttatagct aaattacctg
     2461 agaattatct gaaacttcct ctgctagaac ctttggtaaa agttggtctt ttcgattcat
     2521 ttgaaaaaaa tcgtcaaaaa gtatttaata atttagctaa tctatttgaa tttgtgaaag
     2581 agttgggaag tttgtttgga gatgctattt atagttggca ggaatcggaa gattggacgg
     2641 aacaagaaaa attttatatg gaacaagagc ttttagggat aggtgtcagc aaacatccac
     2701 tacaagctat tgcaagtaag gctatttacc cgattacccc aatcggaaat ttgtcagaaa
     2761 atagctatgc tattatcttg gttgaagttc agaaaataaa agtgattcgt accaaaaagg
     2821 gtgaaaatat ggccttctta caggcagatg atagtaagaa aaaattggat gtcactctct
     2881 tttcagactt atatcgtcag gttggacagg aaataaaaga gggagccttc tactatgtaa
     2941 aaggaaaaat acaatcacgt gatggccgtc tgcaaatgat tgcacaagaa ataagagaag
     3001 cagttgctga acgcttttgg atacaggtga aaaatcatga atcggatcaa gaaatttcac
     3061 gcattttaga acaatttaaa ggcccaatcc cagtcatcat ccggtatgaa gaggaacaga
     3121 aaaccatcgt ttctccccat cattttgtag ctaaatccaa tgaattagag gagaaattga
     3181 atgaaatcgt tatgaaaacg atttatcgct aaaaatacgg aaaatagaag aattttaaaa
     3241 tcaaatgtga tataatcaag aagaatgtta aaagaaaaag gagcataaac caatatgaaa
     3301 cgtattgctg ttttgactag tggcggagac gcacctggta tgaacgctgc catccgtgca
     3361 gttgttcgtc aagcaatttc agaaggaatg gaagtgtttg gtatctatga cggatatgct
     3421 ggtatggttg ccggtgaaat tcatccccta gatgcagctt cagtagggga catcatttct
     3481 cgtggtggta cttttcttca ctcagctcgt tatccagagt tcgctcaact tgaagggcaa
     3541 cttaaaggga ttgagcaatt gaaaaaacac ggaattgaag gtgtagttgt tatcggtggt
     3601 gacggatctt accacggcgc tatgcgtttg actgaacatg gcttcccagc tattggtctt
     3661 ccaggtacaa tcgataacga tatcgttggt actgacttta caatcggttt tgacacagcg
     3721 gttactactg ccatggacgc tatcgataag attcgtgata catcatcaag tcaccgtcgt
     3781 acttttgtaa tcgaagttat gggacgtaac gctggtgata tcgctctttg ggctggtatt
     3841 gcaactggtg ctgatgaaat catcatccct gaagcaggct tcaagatgga agatatcgta
     3901 gcaagcatca aagctggtta tgaatgtggt aaaaaacaca atatcatcgt cttagctgaa
     3961 ggtgtgatgt cagcggctga atttggtcaa aaacttaaag aagctggaga tacaagcgac
     4021 cttcgtgtaa cagaacttgg acatattcaa cgtggtggtt ctccaactgc gcgtgaccgt
     4081 gttttggcgt cacgtatggg tgcacatgct gttaaacttc ttaaagaagg tatcggtggt
     4141 gttgcggttg gtattcgtaa cgaaaaaatg gttgaaaatc caattcttgg tactgcagaa
     4201 gaaggggcat tgtttagcct tactgcagaa ggtaagattg tggttaacaa cccgcacaaa
     4261 gccgatattg agctatctag cttgaataag agcttgtcat aatttacaat ttagttaata
     4321 agaccaaaaa ggtcatatat ataaaggagt cacaaaaatc atgaacaaac gtgtaaagat
     4381 cgttgcaact ttgggtcctg cggtagaaat ccgtggtggt aaaaaattcg gtgaggacgg
     4441 atactggggt gaaaaacttg atgttgaagc ttcagctaaa aacattgcta aattgattga
     4501 agctggtgct aacacattcc gattcaactt ctcacacggc gaccaccaag aacaaggtga
     4561 gcgtatggca actgttaaac ttgcggaaaa aattgcaggt aaaaaagttg gtttccttct
     4621 tgatacaaaa ggacctgaaa tccgtacaga attgttcgaa ggtgaagcta aagaatattc
     4681 atacaaaact ggtgaaaaaa ttcgtgttgc aactaaacaa ggaatcaaat caactcgtga
     4741 agtgattgcg ttgaacgttg ctggtgctct tgatatctat gatgaagttg aagttggtcg
     4801 tcaagttttg gttgacgatg gtaaacttgg tcttcgtgtg gttgctaaag atgatgcaac
     4861 tcgtgaattt gaagttgaag ttgaaaacga tggtatcatc gctaaacaaa aaggtgtgaa
     4921 catccctaac actaaaattc ctttcccagc tcttgctgaa cgcgataacg acgatatccg
     4981 tttcggtctt gaacaaggta tcaacttcat cgcaatttca ttcgtacgta ctgcaaaaga
     5041 tgtgaacgaa gttcgtgcaa tctgtgaaga aactggaaac ggacatgttc aattgttcgc
     5101 taaaatcgaa aaccaacaag gtatcgataa cttagatgaa atcatcgaag cagctgatgg
     5161 tattatgatt gctcgtggtg atatgggtat cgaagtaccg ttcgaaatgg ttccagttta
     5221 tcaaaaaatg attatcaaga aagtcaatgc tgcaggtaaa gttgttatca ctgcaacaaa
     5281 catgcttgaa acaatgactg aaaaaccacg tgcaactcgt tcagaagtat cagacgtatt
     5341 caacgctgtt atcgacggaa ctgacgctac aatgttgtca ggcgagtctg caaacggtaa
     5401 atacccactc gagtcagtaa ctacaatggc tacaatcgac aagaacgctc aagctcttct
     5461 taatgaatac ggacgtcttg attcagattc atttgagcgt aactctaaga cagaagtaat
     5521 ggcttctgct gttaaagatg ctactagctc aatggatatc aaattggttg taactcttac
     5581 taagacaggt catactgcac gtttgatttc taaataccgt ccaaatgctg acatcttagc
     5641 attgacattt gacgaattga cagaacgtgg cttgatgttg aactggggtg ttatcccaat
     5701 gttgacagat gctccatctt caactgacga tatgttcgaa atcgctgaac gtaaagcggt
     5761 agaagcaggt ctcgttgagt caggcgatga tatcgttatc gttgctggtg tgccagtagg
     5821 agaagctgtt cgcacaaaca caatgcgtat ccgcacagta cgttaagaaa aatataaaaa
     5881 cctatcatat ccagctttct agcctgtatg atgggctctt tttttgtcca gggggcaaaa
     5941 agggggcaaa actttcataa tggattgata ctcttcgaaa atctcttcaa accacgtcag
     6001 cgtcgcctta ccgtatatat gttactgact tcgtcagttc tatctacaac ctcaaagcag
     6061 tgctttgagc aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtatgaa
     6121 ataagatatg cataaattga ttagaaagtc aaatgaattt ctacaaatgt tttagcaatc
     6181 gcaatctact tgtctagatt cgatctgata tattttcgat ttaatgatat ggtatttaaa
     6241 acctccaaag tagcttgctc cattctttta cctacgtgag tctagatgtt atttactgtt
     6301 ttagcgtttt tgtgttctac tctaaccatt atagcattct tctcagctag tgtactaagg
     6361 agtgtgtgcc tgaaaatatg ggaactaagg ggctggttta tcggtttctc tagtttagta
     6421 tttgcctttt gcaaagtgat cttaaatgcc tttctctaaa tttacatatc actattgttt
     6481 aacaaaatct aatctatttt aggtcactta ttcttttttt gaaatgtaga atgaactttt
     6541 tcaaagtttt tcgaatcttt taaaatctgt ttgttttata tcgccattct cccccttttt
     6601 taattctccc tatatagcct gacaactttt ccgatggtac gaatatggtt gctttcgtct
     6661 aggtggatgt cggagtattc gggattgagt ttttttgagg tagccttggc ggagtttctt
     6721 gacatagtta gtgccgtcta cttggaagat gccgatggta ttatagtcaa tctgtggggt
     6781 attcttgata aataggtagt cgctgtttct tatctttggc tccatggact tgctgacgac
     6841 ataagcgatt gggtcgtagt cgtctgggat aatggaaact ccataactaa atcgtcgtcc
     6901 tgcatcaagc ggctacctgc agagataaac tacctaacac gagagtaagt agtctgtctg
     6961 tagtcgtcca gtctgatgat ttttacgata cttcgttttt ctgatcatac agttgcctct
     7021 cggcataggt cagaacttta ccttgtctgg gtggtgcccg ttggtcgtag atagattgga
     7081 tatcgctagg agaatccttt tgaactggag gaaagagggc atcaatcaag ctactgaata
     7141 ctttaactaa gtcaaatata gtatttttct tagtagacct aacccttttt tcataatttc
     7201 taatggtgtt tttacttata cctatcttag tacccaattc ttattgagtc caaccattac
     7261 tagtctatat tgttttatag ttgattgagt ttggaatagt acgctgtagc tgctaaaaca
     7321 tttctaggaa ttaatttgcc tttcctaata gagttgttca tatcttattt caatctacta
     7381 tatttttcac ctctaacaat cgcaatctct tctttatcca tgaacgaaaa cgctttctat
     7441 ctttgtaagt aaagcatcac acgaaatcca cgaaaatgaa aacctttgtt gtgttttcgt
     7501 aaaaaatttg ttgacagagc acgaaacgcc tactataatt aaatcaagtt taaggaaata
     7561 acaaaaaaac aaatcggagg gaaagacgat gaacacatta aatgagaaag taatcaatat
     7621 ctgtaaagca gtagttaaag aaactttaat ccaagacatt taggagaaag aaatgaagaa
     7681 atcaagaaaa ttagccacat taggaatctg ttcagccttg tttttaggct tggctgcttg
     7741 tcaacaacaa catgctactt ctgaggggac gaatcaaagg caaagcagtt cagcgaaagt
     7801 tccatggaaa gcttcataca ccaacctaaa caaccaggta agtacagaag aggtcaaatc
     7861 tctcttatca gctcacttgg atccaaatag tgttgatgca ttttttaatc tcgttaatga
     7921 ctataatacc attgtcggct caactggctt atcaggagat ttcacttcct ttactcacac
     7981 cgaatacgat gttgagaaaa tcagtcatct ctggaatcaa aagaagggcg atttcgttgg
     8041 gaccaactgc cgtatcaata gttattgtct tttgaaaaat tcagtcacca ttccaaagct
     8101 tgaaaagaat gaccagttgc ttttcctaga taatgatgcg attgataaag gaaaggtctt
     8161 tgattcacaa gataaggaag agtttgatat tctattttcg agagttccaa ctgaggcaac
     8221 tacagatgtc aaggttcacg ctgaaaagat ggaaacattc ttctcacaat ttcaattcaa
     8281 tgaaaaagct cgaatgctgt ctgtagtctt gcacgacaat ctggatggcg agtatctgtt
     8341 tgtaggccac gttggggtct tagtacctgc tgatgacggt ttcttatttg tagagaaatt
     8401 gactttcgaa gagccctacc aagcgattaa atttgctagt aaggaagatt gctacaagta
     8461 tttaggcacc aagtatgcgg attatacagg cgagggactg gctaagcctt ttatcatgga
     8521 taatgataag tgggttaaac tttaatagtg ttatgatatg acagtaatct taggattttt
     8581 atttctattt ttgatgagtt ggtgggttat ttcaatcgtt aatagccaga aaccacgcaa
     8641 aaatgaaaca tttattggct atatccaaag atacgatatt gatgggaatg gctatcaatc
     8701 attaccgtgt cctcagaact gagaggagtt cttgaaatcg taaaactact ttgaacaaga
     8761 gttacttcaa cccattggct ccgacggatt aagttgcttt cgtgaacacc aaaatcagcc
     8821 gcaatttctt cataagtgcg gtattctcgc acatattgaa gagtggccat aagaaggtct
     8881 tctaggctta atttaggttt tcgtccccct tttgtgtgtt taagttgata agcagttttt
     8941 aatacagcta acatctcttc aaaagtggtg cgctgaacac caacaagaca tttaaatcgt
     9001 gcatcagtta gttgcttact tgcttcataa ttcatagaac tactatacca tattttgttt
     9061 cgcaggaagt ctaatgctaa aatcttatca gtgagaagat ttcttaaagt ttattctttg
     9121 tagtttcatc tgtattagta cataatttaa cttagagaaa ataggaggac taatcgaatg
     9181 gaattaaaag attttacaga aaaagaacag gaaatgatta agaaaaggct tacaatgtct
     9241 aatattagtg acaaagaaac tactgagaag attctggcgc tagtaccaca agacttgatt
     9301 aagcgaatcc cgttttttgt cagaaaacat gctacaacac gtacgattaa acgcatttca
     9361 attgaacacc ctgaactcta cgctgtagct caaacaagtg gtgagattcc agaaaaaaaa
     9421 acgcgaagaa ttgcgtcaga ttatcacaac tatctttgaa caaaagatga ataagcatag
     9481 tattaagtag agaatgaaaa aatattttat tggcggtttg ggaagcaatg cctatcatag
     9541 caaggatttt ctttaagaac tagattcgca ggtctatttt ctaaatccat atgaaaagca
     9601 tcttcgagat gaaacagaat tgaaatcatg gtttaaaaat gagattgtag aggaagaatc
     9661 tatctgtctg ataggtcatt ctattggagg cgatttagct cgttatttcg catcggaatt
     9721 tgaagaagta aagaaactga ttcttttgga tggtggctat ctagatttag ataagatttt
     9781 acctttggat acagagttag aggaaactaa aaattatatc aaatctcaaa ttatttcgga
     9841 cttagatgtt cttacttcta aagaaaaatc tgaagcaaag cattggtcag aaaatatgga
     9901 gaaagctgta agacagtcct atcactggaa tgttgagtat aatagatatg agttggctat
     9961 aaattatgaa aatatagaag cgatactccg cctacggaga aaaatacaag cttttaagag
    10021 agaagtggga gataccttgt ttattagtcc tcgctatcct aatgaagcta catggagaga
    10081 ggaagcccta aaagaattgc cagactattt tgatactatt tttttagaaa actttggcca
    10141 tgagctttat actcaagcac ctaaagaaat cgctagtcta atgaatgagt ggctcgctta
    10201 ttttctatga gctacttgta ttgctggaaa tggtataaga tgaaaatcta caagtcacta
    10261 attttcatat aaccaaaaaa cgaacaggct ctttttctgt tattcagaag agaacttgtt
    10321 cgtttttatc ttgattttct taaaaagctc tatatacata gggattttct ggtttatcga
    10381 ctttggtaaa gctgtcgatt tccaaggttg gaaggttttg tttgagttct ttatggtagt
    10441 gattgaccag ttttcctttg gctgaaatcc aagtgttgtt ttcatactgc cgggtattgc
    10501 ccttggtcag caatccatag acaccagaat ctgcgataca gtggataatg ccgaatcgga
    10561 acagaaattg actattggca tgactggggt cgttatagac aaagcctgta aactggattg
    10621 tcttgccctc aaactcatct ggatagtcgt agatagcctc catgacttcc atatagtttt
    10681 cattagtgat ctgaatgctg tcttgggata agtatttatc cgccgccgtt cgcatttcct
    10741 tttcataggc tgattttgaa aaataagaac tagtatctgg tttcaaatat tggcttgtag
    10801 tcccttcact tgtctgaata gccagattcg ttccttccga tagagggaaa tgatagcctt
    10861 tagctgaaac agtctgagaa tccaagctaa cagttgggaa agttaagccg atgacaagtg
    10921 gaatagccag aagagaaata ctcgtcatct tggccaatcg gctgttcaga tgactgtggg
    10981 ttttgacttg cttcatccag atatacaatt gaacgatagc caagataaaa gaaagcacca
    11041 tggagatata ggccagatag gaatagtgca tgttgatgta ctggtttagt ttgcccgaca
    11101 gatggaggta aatagtcagt tcaaaatagc cagctaaaac taaaaatcga atcataggat
    11161 gactccaatc aagagagaat agactaagac gacaagagtt acaattgtta tgaagtgact
    11221 gataaatcgt gctttcaagt aatttttcat catgagaata tttttgatat ccagcattgg
    11281 accaatgacg agaaaggcca gaactggtgc caaaccgaaa ctcgagagaa gagaagcacc
    11341 tataaaggca tccgcctcac tacagagcga aagaagaaag gctaaaatca tcaagagcag
    11401 gatggcaaaa agaggggtcg cactgataga ggtcagaatc cgagtcggaa cgtagacctg
    11461 tattatagaa gcaaagaggc agccaaatac caaataacgc cccgtatcaa aaaattcatc
    11521 aatggcctgc acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag aaaaatcatg
    11581 ctcatgacaa gccagacgat tttctttctg aatcggttct tgccagaaaa atcctagaaa
    11641 aattcctagt attacagcca caagaatgga acccagagct cgtaataggg cgacatggaa
    11701 ggagttgcca aaggcagaat aggtcgcaaa aagaacaatg ggattgataa caggtgctgt
    11761 cacaagaaaa ggaacggccg tgtaacttgg aacctttttt tccagaaaac gattgatgat
    11821 ggggacgatt ccacattcac aagaagggaa aagtataccg acaaaggtcc caaaaaagat
    11881 tctcccccaa cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga tataaacttc
    11941 aatcagccct gagacaatgc ttcctatcag aacgaaggga agggcttcaa tgataatgga
    12001 gagaaaaatg gctccagttt gaagtacact agatgggagt tgttgaaaag cagtcatcta
    12061 gtttttcttt tctgctttga cttttttctc tttgtcatca gggaaagtaa cttgtttcaa
    12121 atcagggagt ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt ttgtaaattt
    12181 gacagccata ggtgtacctc gattctaaat tctacctcta ttatactatt attttgcgga
    12241 aaagggagtt ttttaatttg ttttcgatag ttgatactct tcgaaaatct cttcaaacca
    12301 cgtcagcttc gccttaccgt agta
//
LOCUS       DAVLSL010000049        12300 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLSL010000049 DAVLSL010000000
VERSION     DAVLSL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.050"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(75..824)
                     /locus_tag="VXN30_001067"
     CDS             complement(75..824)
                     /locus_tag="VXN30_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1357698.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDCSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            957..3293
                     /locus_tag="VXN30_001068"
     CDS             957..3293
                     /locus_tag="VXN30_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1357699.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKLINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3395..4072
                     /locus_tag="VXN30_001069"
     CDS             3395..4072
                     /locus_tag="VXN30_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1357700.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPIFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4059..4856
                     /locus_tag="VXN30_001070"
     CDS             4059..4856
                     /locus_tag="VXN30_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1357701.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4873..5976
                     /locus_tag="VXN30_001071"
     CDS             4873..5976
                     /locus_tag="VXN30_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1357702.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            5983..6174
                     /locus_tag="VXN30_001072"
     CDS             5983..6174
                     /locus_tag="VXN30_001072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357703.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            6277..7296
                     /gene="galE"
                     /locus_tag="VXN30_001073"
     CDS             6277..7296
                     /gene="galE"
                     /locus_tag="VXN30_001073"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1357704.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7298..8263
                     /locus_tag="VXN30_001074"
     CDS             7298..8263
                     /locus_tag="VXN30_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1357705.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(8314..8520)
                     /locus_tag="VXN30_001075"
     CDS             complement(8314..8520)
                     /locus_tag="VXN30_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1357706.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            8572..9048
                     /locus_tag="VXN30_001076"
     CDS             8572..9048
                     /locus_tag="VXN30_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1357707.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSHPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9057..9728
                     /locus_tag="VXN30_001077"
     CDS             9057..9728
                     /locus_tag="VXN30_001077"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1357708.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9859..10197
                     /locus_tag="VXN30_001078"
     CDS             9859..10197
                     /locus_tag="VXN30_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1357709.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10200..10829
                     /locus_tag="VXN30_001079"
     CDS             10200..10829
                     /locus_tag="VXN30_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1357710.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11069..12220
                     /locus_tag="VXN30_001080"
     CDS             11069..12220
                     /locus_tag="VXN30_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1357711.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
BASE COUNT         3400 a         2379 c         2897 g         3624 t
ORIGIN      
        1 tagaggtgta ctattctagt tccaatctac tatataaatg aaaaccttag ctgtttggcc
       61 aaggttcatt tcttctatgc aagttcttct cttttcttat ctgggttcca gatgaagctt
      121 gcgataaagc taaagatgat ggttaggata gcgaggataa tagcaaggat gagggcaggg
      181 atgcggataa accaccagag tggatttggt tttttatcat tgtgccattg tttcgtttct
      241 tcgtccagac gttggaattc tgtttgaata cgattggcga ctgtttcaat gccttcatca
      301 ttcattttct tgatatctga gatatcgatt ggatttccaa agttcatatc gacacgttca
      361 cggctaatca agcccttcaa agtcatggga ccggtgtagg taaccggcat gatacggacc
      421 ttggccattt tggcaatcag tgctgcgccc cccttgacat cgtttgagtg gcggctacca
      481 cttggaaaca tgatgagaga gcagtcactt tttttgagaa cgttgatagg atatttgatg
      541 gctgaggcgc tgggattttc acggtcgatg ggaaaggcgc cacacatacg aatccaccaa
      601 ccaaagatac ggttggtaaa gagttctttt tttgccataa agatgaactg ttttggcttg
      661 gtcgcaaagg ccatataaac aggatcccac caggtacggt gaggcgcaac taaaatataa
      721 ttttcatctt gattaggaat tttatcagta ttatgatagt gagcattgcc attgatggac
      781 cataagagca atacaactaa tccacgcaaa taagtataaa acatgcgatc tccttcaatt
      841 gttttcttgt tattattata ccttatcaaa ggagggctgg caaacttttc ccttgactag
      901 atgcatattt gggatgaaat tagaattctg ttaaaaaaaa tgatataata gaatttatgg
      961 ataaaaataa gattatggga ttaacccaaa gagaagtcaa ggaaagacag gctgagggtt
     1021 tggtcaatga ctttaccgca tcagccagta ccagcacttg gcaaatcgtt aaacgaaatg
     1081 tctttaccct ttttaacgct ttgaactttg ccattgcttt ggcccttgcc tttgtgcagg
     1141 cttggagcaa tctggtcttc tttgctgtta tctgctttaa cgctttttct gggattgtga
     1201 ccgagctacg agccaaacac atggtggaca agctcaatct catgaccaag gaaaaggtca
     1261 aaaccatccg tgatggtcag gaagttgctc ttaatcctga agaattagtg ctaggagatg
     1321 tcattcgttt gtctgcagga gagcagattc ctagtgatgc cttggttttg gaaggctttg
     1381 cggaagtcaa tgaagccatg ttaacgggag aaagtgattt ggtgcaaaag gaagttgacg
     1441 gcttactttt gtcaggaagt ttcctagcca gtgggtcagt tttatctcaa gttcaccatg
     1501 tcggtgcaga caactatgct gccaaactca tgcttgaggc taagaccgtt aaactcatca
     1561 actcccgtat catgaaatcg ctggacaagt tggctggttt tactgggaag attatcattc
     1621 cctttggtct ggctctcttg ctggaagcct tgcttttaaa aggcctgcct ctcaagtcat
     1681 ctgttgtaaa ctcgtcgaca gctcttttgg gaatgttgcc taagggaatt gcccttttga
     1741 ccattacttc gctcttgact gcagtgatta agctaggttt gaaaaaggtc ttggtgcagg
     1801 agatgtactc tgttgagacc ttggcgcgcg tggatatgct ctgtctggac aagacgggta
     1861 ccatcaccca aggaaagatg caggtggagg ctgttcttcc actgacggaa acttatggtg
     1921 aagaggctgt tgccagcatc ctgaccagct atatggccca tagtgaggat aaaaatccaa
     1981 cagctcaagc cattcgccag cgttttgtgg gagatgttgc ttatcctatg atttccaatc
     2041 ttcccttctc gagcgaccgc aagtgggggg ctatggagtt agaaggtttg ggaatagttt
     2101 tcttaggggc acctgagatg ttgctggatt ctgaagtccc agaagccagg gaggccttgg
     2161 agagaggatc acgtgtcttg gtcttagctc tcagtcagga gaaattagac catcacaaac
     2221 cacagaaacc atctgatatt caggctctag ccttgctgga aatcttggac cccattcgag
     2281 agggagcagc agagacgctg gactatctcc gttctcagga ggtgggactc aagattatct
     2341 ctggtgacaa tccagttacg gtgtccagca ttgcccagaa ggctggtttt gcggactatc
     2401 acaactatgt agattgctca aaaatcaccg atgaggaatt gatggccatg gcggaggaga
     2461 cagcgatttt cggacgtgtt tcccctcatc aaaagaaact catcatccaa acgttgaaaa
     2521 aagcgggtca tacaacggct atgacagggg acggggttaa tgatatcttg gcccttcgtg
     2581 aggcggattg ttctatcgtg atggcggagg gggatccagc aacccgtcag attgccaatc
     2641 tggttctctt aaactcagac tttaatgatg ttcctgagat tctcttcgag ggtcgtcgcg
     2701 tggtcaataa cattgcccac atcgccccga ttttcttgat aaagaccatc tattccttcc
     2761 tgttagcagt catctgtatt gccagtgctt tactaggtcg gtcagagtgg attttgattt
     2821 tccccttcat tccgatccag attaccatga ttgaccagtt tgtggaaggt ttcccaccat
     2881 tcgttttgac ttttgagcga aatatcaaac ctgtcgagcc aaacttcctc agaagatcca
     2941 tgcttcgtgc cctaccaagt gccctcatgg tcgtgtttag cgttcttttt gtgaaaatct
     3001 ttggaacgag tcaaggttgg tctgagttag aaatctcaac tctcctctat tatctcttgg
     3061 ggtcaattgg tttcttatcc gtatttagag cctgcatgcc attcactcta tggcgagtcc
     3121 tcttgattgt ctggtcagta ggaggcttcc tagccacagc tctcttccca agaatccaaa
     3181 aactgcttga aatttcaacc ttgacagaac aaacgttgcc tgtttatggt gtcatgatgt
     3241 tggtctttac agtgattttc atcctgacta gtcgctatca agctagaaaa taaagaaaga
     3301 ctgcaatctg tggattgcag tttttttagg tacaagattg ccagccgaaa tgtggtataa
     3361 taagaggtaa tagagttttg gaaagtgaga gaagatgatt tcaaagagat tagaattggt
     3421 agcttccttt gtgtcacagg gggctatttt actagatgtg ggaagtgacc atgcttatct
     3481 gcctatcgag ttggttgaga gaggccaaat caaaagcgct attgcaggtg aggtggtgga
     3541 aggtccctat cagtctgcgg ttaaaaatgt tgaggctcac ggcctaaagg agaaaatcca
     3601 agtccgttta gccaatggct tggcagcttt tgaagagact gaccaagtgt ctgtcattac
     3661 cattgctggc atgggtggtc gtttgattgc taggatttta gaagaaggtt tggggaagtt
     3721 agctaatgta gaacgtttga tcctccagcc caataatcgt gaagacgact tgcgtatctg
     3781 gctacaggat catggattcc agattgtagc agaaagcatc ttagaagaag ctggaaagtt
     3841 ttatgagatt ttggtggtgg aagcaggaca aatgaagcta tcagccagtg atgttcgctt
     3901 tggtcccttc ttgtccaaag aagtcagtcc aatatttgtc caaaaatggc aaaaagaagc
     3961 tgagaagcta gagttcgccc tcggacaaat cccagaaaaa aatctggaag aacgtcaagt
     4021 tctagtagat aagattcaag ctatcaagga ggtgctccat gttagcaagt gaagtgatcc
     4081 aagcttatga agccttttgc cctcaggaat tttctatgga gggagacagc tgtggtctgc
     4141 aaattggcac tttagacaag ggtatccaaa gggtcatggt ggctctcgat attcgtgaag
     4201 agacggtggc tgaagccatt gaaaagggtg tagacttgat tatcgtcaag cacgcgccta
     4261 tctttcgtcc tatcaaggac ttgcttgcca gccgtccgca aaatcagatt tacatcgacc
     4321 tgattaagca tgacatcgca gtttatgtca gccataccaa tattgatatc gttgaaaatg
     4381 ggctcaatga ctggttttgt cagatgctag gaatcgagga gacgacttat ctgcaggaaa
     4441 caggtccaga acgtggaatt ggacgtattg ggaatattca gcctcagaca ttttgggaat
     4501 tggcccaaca ggtcaagcaa gtctttgacc tagatagcct tcgaatggtg cattatcaag
     4561 aggatgattt gcagaagcct atttcaagag tagcaatttg tggtggaagc gggcagtctt
     4621 tctataagga tgctttggca aagggggcag atgtctatat cactggtgat atctactacc
     4681 atactgctca ggatatgctg tctgatggct tgttagcatt ggatccaggt cactatatcg
     4741 aagtgctttt tgtggaaaaa atcgcaacac tccttactca atggaaagag aaaaaaggct
     4801 gggaactaga gattttacct agtcaagcat cgaccaatcc tttccgtcat atctagttag
     4861 aaagtgaaga caatgaaaaa agttgccatt attggagcag gaattgtggg agcaacagct
     4921 gcctactacc tctcgagaga aagtgaccta gaggtgaccg tttttgacca tgagcaaggt
     4981 caagccacca aggccgcagc aggaattatc agtccttggt tttccaaacg ccgtaataaa
     5041 gcctggtaca agatggcgcg cttgggggct gatttttatg tggatttatt agctgattta
     5101 gagaaatcag gacaagaaat cgacttttac cagcgttcgg gagtctttct cttgaaaaag
     5161 gatgaatcca atttggaaga actttatcaa ctggccctcc agcgcagaga agaatctccc
     5221 ttgatagggc aattagccat tctgaaccaa gcctcagcta atgaattatt ccctggtttg
     5281 cagggatttg accgcctgct ctatgcttct ggtggagcga gagtagatgg tcacctctta
     5341 gtaactcgtt tgctggaagc cagtcaggtc aagttggtca aagaaaaagt gactctgaca
     5401 ctcttatcat caggctacca gattggcgaa gaggtgtttg atcaggttat tttggcaacg
     5461 ggagcttggt tgggggacat gttagagcct ttaggttatg aagtggatgt ccgtcctcaa
     5521 aaaggacaac tacgagatta tcagcttgcc caagacatgg aagattaccc tgttgtcatg
     5581 ccagaagggg agtgggattt gattcccttt gcaggtggga aattatcctt gggcgctacc
     5641 catgaaaatg acatgggatt tgatttgacg gtagatgaac ccttgctcca acaaatggag
     5701 gaggcggcct tgcctcatta cccagtttta gctagagtga cttcaagggc tgagcgtgtg
     5761 ggaatccgtg cctataccag tgatttctct cccttctttg ggcaggtgcc agaattggca
     5821 ggtgtctatg cagccagtgg actaggttca tcaggcctca caactggtcc tatcattggt
     5881 taccatctag cccaactgat ccaagacaag gagttgacct tggaccctga aaattaccca
     5941 attgaaaact atgtcaaacg agtaaaaagc gaataatact caatgaaaat caaagaacaa
     6001 actaggaaac tagccgcggg ttactcaaag cactgctttg aggttgtgga tagaactgac
     6061 gaagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat
     6121 acccatggca aggcgacgtt gacgtggttt gaattggatt ttcgaagagt ataagcattt
     6181 tactgaaatt ttatcaggta gtttaggatg gcaaatgaca ttccctgtca aaaatggtaa
     6241 aataagaaaa aatgatctga gaatcgagga aaaaagatgc aagaaaagat tttggtaact
     6301 ggtggtgccg gttttattgg aacccacact gttattgaat tgatccaagc aggccatcaa
     6361 gttgttgtgg tggataatct tgtcaatagc aatcgtaaga gtttagaagt tgttgaaaga
     6421 atcacaggag ttgagattcc tttctatgag gcagatattc gtgacacaga taccctcaga
     6481 gatattttca agcaggaaga accgactggt gtcattcact ttgctggttt gaaggctgtt
     6541 ggcgaatcaa cacgtatccc tcttgcctac tatgacaaca atatcgctgg aactgtcagc
     6601 cttttgaaag ccatggaaga aaacaactgt aaaaacatca tcttcagttc ttctgcgaca
     6661 gtttacggag atccgcacac agtgcccatc ttggaagatt tcccactttc agtgacaaac
     6721 ccatacggtc gtaccaagct catgctagag gaaattttga ccgatattta caaagcagac
     6781 tcagaatgga atgttgtctt gcttcgttac tttaacccaa tcggagccca tgagagtggt
     6841 gatttgggag aaaatccaaa cggtattcca aacaatctct tgccatatgt gactcaagta
     6901 gccgttggaa aattagagca agtgcaagtg tttggagacg attacgatac ggaagatgga
     6961 acaggtgttc gtgactatat ccacgttgtc gatttggcta agggtcacgt tgcagctttg
     7021 aaaaaaatcc aaaaaggttc aggactaaac gtttataacc ttggaactgg taaaggttac
     7081 tcagttcttg aaattatcca aaacatggaa aaagcggtgg gacgtcctat tccttaccgc
     7141 atcgtagaac gtcgcccagg tgatatcgct gcctgctact cagacccagc aaaagctaaa
     7201 gcagaactcg gttgggaagc agaactcgac atcacccaaa tgtgtgaaga cgcatggcgt
     7261 tggcaaagca agcatccaaa tggatttgaa gactaagatg atgatttcaa tcatcgtccc
     7321 ttgtttaaac gaagaggaag tacttcctct tttttatcag gctctggaag ctttacttcc
     7381 agatttggaa acagaaatcg agtatgtctt tgtcgatgat ggatcaagtg atgggacctt
     7441 ggaactctta aaggcctatc gggagcaaaa tccggcagtc cattatattt ctttctctcg
     7501 aaattttggc aaagaagcag ccctttatgc aggcttgcaa tatgcgacag gagatttggt
     7561 ggtggtgatg gatgcagacc tccaagatcc tcctagtatg ttgtttgaga tgaaaaatgt
     7621 actagacaaa aatgtagact tggactgcgt tgggacacgg agaactagtc gggagggaga
     7681 acccttcttt cgcagtttct gtgctgttct cttttatcgc ctcatgcaaa aaatcagccc
     7741 agtagctctg ccgtcgggtg tccgtgattt tcgtatgatg agaaggtctg tagtcgatgc
     7801 cattttaagc ttgaccgagt ccaatcgttt ttcaaagggg ctcttcgcct gggtgggttt
     7861 caaaacctac tatttggact atccaaatgt cgaaaggcag gctggcaaga caagttggag
     7921 ttttaggcaa ctctttttct actccattga agggattatt aatttttcag attttccctt
     7981 gattatagcc tttgtagctg gtctcctatc ttgttttctt tctctgctga tgaccttttt
     8041 tgttgtggtt cggaccctca ttttgggcaa tccgacatct ggttggacct ctctgatggc
     8101 tgttattctc tttcttggag gcattcaact cttgaccatt gggattctcg gcaagtatat
     8161 cagtaagatt tatttagaga ctaaaaaaag accactttat cttatcaaag aaaaaagtga
     8221 ccttcctgat tttacagaaa aaaataaagt gaaaagacta taattttaca tggaaatgtg
     8281 ctaaactaga gggagtggat tatcgccctt gatttaggct tcctggtttc caatcagggc
     8341 gcgagttggg caatttttaa cagcctctaa gacatcctga ctaggagaaa tttctttttc
     8401 cagttggtca gggtcatcgt aaaaacgcac gattccatta tcgtggtaat caaataaatc
     8461 agaataagtt tggcaaagcc cacaggcgat acatcgttca ggtataagtg tgattttcat
     8521 gttcatattt atattgtaat aagaaaagta aaaaaaaaca aggagtaagg tatggcaaaa
     8581 gaaccgtggc aagaagatat ctatgatcaa gaagaatcaa gagcagagcg tcggcatcga
     8641 aaccacggag gggctgatag gatggctaat cgtattttga cgatcctagc tagtattttc
     8701 tttgtaattg tggtggtgat ggtcatcgtt ctcatctatc tatcatcggg gggaagtaat
     8761 cgcacagcag ccttaaaaga ctttcatgat tctgatgcaa gtgtagtaca aatctcatct
     8821 tcaagcagtt ctcatcctga gcagagttca gagccagaat ctacttctag tagttcagaa
     8881 gaagctgcta atcctgaagg aacgattaaa gttctcgcag gagaagggga agcagctatt
     8941 gccgctcgtg caggaatctc cattgctcag ttagaggcct tgaatcctgg gcacatggct
     9001 acaggatctt ggtttgctaa tccaggtgat gttataaaaa taaaatagga gccgatatga
     9061 aaacaattca aattgctatt gatggtcctg cttccagcgg taagagtacg gtcgcaaaga
     9121 ttattgctaa ggattttgga ttcacctacc ttgatacagg agctatgtat cgtgcagcga
     9181 cctatatggc tcttaagaac caattaggag ttgaagaagt cgaagccctt ctagccttgt
     9241 tggaccagca tccaatcagc tttggacgtt cagaaactgg agaccagctt gtttttgtag
     9301 gagatgtgga tattacccat cctatccgtg aaaatgaagt gaccaatcat gtttctgcta
     9361 ttgcagcaat tcctgaagtg cgtgagaaac tggtttctct ccaacaagaa attgcccagc
     9421 aaggcgggat tgtcatggat ggtcgcgata ttggaactgt tgtattgcca caagcagaat
     9481 tgaaaatttt cctagtagct tctgttgatg agagagcaga gcgtcgttac aaggaaaata
     9541 ttgccaaggg aattgaaaca gaccttgaaa ccctaaaaaa ggaaattgct gcgcgtgact
     9601 acaaggatag tcatcgtgag acttctcctc tcaaacaagc agaggatgct gtctaccttg
     9661 atacaactgg tttgaacatt caagaagtag ttgaaaaaat caaagcagaa gctgaaaaaa
     9721 gaatgtaaat gtagaaaagc cgatagattg atatcggttt ttttctaaat ttgtcaaaat
     9781 gctgatttta gggtataata gttgctgttc gagaaatttt gattttcaaa gaatagtgaa
     9841 tgatgataag gagaaaccat gaacaattta ccaaattgtc caaaatgtaa ctcagagtat
     9901 gtctacgaag acggtgccct actggtttgc ccagagtgtg ctcatgagtg gaatcctgct
     9961 gaagttgcag aagtagaaga aggtcttgtc gctatcgatg ccaacggaaa taaattgact
    10021 gatggtgata cagtaactct catcaaggac ttgaaagtaa aaggtgcgcc aaaagatttg
    10081 aaacaaggga cgcgcgtgaa aaatatccgc atcgtagaag gcgatcacaa tatcgactgt
    10141 aagattgatg gttttggtgc catgaaactt aaatcagagt ttgtgaggaa gatttaagca
    10201 tgtcaaaaca ctataaactt gtattttata gccgtatctt cttgtttcta gcggctttta
    10261 cgggagttta tcttgaaatt actaggcatg gtggttttgg gatgcttctc tattacacgg
    10321 ttctgtccaa cctcttggta actattttta ccctttatct tctaaaggtt atgagccgtg
    10381 taggtgaaaa ttggcaaagg ccaagtctct tgcgcttaaa aggtggggtc accatgagta
    10441 tcatgattac ctgtgtgatt taccatttcc tcttggcgcc cattgcgact aatttctata
    10501 ccctagaaaa tttcctttgc cactatatcg ttcccatctg gtttttagcg gataccctct
    10561 tttttgacaa acagggtcaa tacaagattt gggatccagc agtgtggacc attttaccct
    10621 ttctgtatat gatgtttgct ctttttaatg gcttggttct aaaactcaat attccaaatg
    10681 ccaaggataa tcctttccct tacttctttt tgaatgtgaa caagggttgg aatgttgtgt
    10741 ttaagtggtg tctgattatc tttgttgcct atatggtagc aggatttatt ttctacttta
    10801 tcaagcaaat caagagaaag tcatcctaat actcttcgaa aatctcttca aaccacgtta
    10861 gtttccatct gcaacctcaa aacactgttt tgaacaacct gcggctagct ttctagtttg
    10921 ctctttgatt ttcattgagt atgagataca agggattcat tttcgaattt aagaaggagt
    10981 ctacaaacca atgaggcttc ttcttttctt gcttatttga taactatcaa aaaagtagaa
    11041 gattaagaga aatatagaaa gagatttcat gaaacaattt ttagaacggg ccagcatttt
    11101 ggctctctcc ctcgttttga ttacctcctt ttccatttcg agtgccctac cagccatgtt
    11161 tgactattat cagggttatt ctaaggaaca aattgagctc ttggtgagct tgccttcctt
    11221 tggaatcatg atgatgttac tgctaaatgg tttcttagaa aaaatatttc ctgagcgctt
    11281 acagattagt ttgggcttgc tgattttatc attgagcggt acagctccct tctggtacca
    11341 agcctatccc tttgtctttg gaacacggct tctctttggt ttgggtcttg ggatgatcaa
    11401 tgccaaggcc atttctatta tcagtgaacg ctaccaagga aaaaggcgaa ttcagatgtt
    11461 agggctacgc gcttctgcag aggtcgttgg agcttctctc attaccttgg ccgtcggtca
    11521 gttgttggcc tttggttgga cagctatctt tctagcctat agtgctggat ttttggtgat
    11581 gcccctttat ctgctctttg tcccttatgg aaaatcaaag aaagaagtca agaaaagagc
    11641 gaaggaagca agtcgtttaa ctcgagaaat gaaaggcttg atttttacct tagctatcga
    11701 agcggcagtt gtagtttgta ccaatacagc tattaccatc cgtattccaa gtttgatggt
    11761 ggaaagagga ttgggggatg cccagttatc tagttttgtt cttagtatca tgcagttgat
    11821 cgggattgtg gctggggtga gtttttcttt cttgatttct atctttaaag agaaactgct
    11881 cctctggtct ggtattacct ttggcttggg gcaaatcgtg attgccttgt cttcatcctt
    11941 gtgggtggta gtagcaggaa gtgttctggc tggatttgcc tatagtgtag tcttgacgac
    12001 ggtctttcaa cttgtctctg aacgaattcc agctaaactc ctcaatcaag caacttcatt
    12061 tgctgtatta ggctgtagtt tcggagcctt tacgacccca tttgttctag gtgcaattgg
    12121 cttactaact cacaatggga tgttggtctt tagtatctta ggaggttggt tgattgtaat
    12181 ctctatcttt gtcatgtacc tacttcagaa gagagcttag gattgattcc taagtttttc
    12241 ttttgatact caatgaaaat caaagagcat agttgattga gtttggaata gtacgctgta
//
LOCUS       DAVLSL010000050        12288 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLSL010000050 DAVLSL010000000
VERSION     DAVLSL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.051"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..582
                     /locus_tag="VXN30_001081"
                     /pseudo
     CDS             1..582
                     /locus_tag="VXN30_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            773..3064
                     /gene="pcrA"
                     /locus_tag="VXN30_001082"
     CDS             773..3064
                     /gene="pcrA"
                     /locus_tag="VXN30_001082"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1357712.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIVDTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     VAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            3107..3787
                     /gene="radC"
                     /locus_tag="VXN30_001083"
     CDS             3107..3787
                     /gene="radC"
                     /locus_tag="VXN30_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1357713.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3784..4473)
                     /locus_tag="VXN30_001084"
     CDS             complement(3784..4473)
                     /locus_tag="VXN30_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1357714.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(4491..5132)
                     /locus_tag="VXN30_001085"
     CDS             complement(4491..5132)
                     /locus_tag="VXN30_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1357715.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(5215..5430)
                     /locus_tag="VXN30_001086"
     CDS             complement(5215..5430)
                     /locus_tag="VXN30_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1357716.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(5438..5785)
                     /locus_tag="VXN30_001087"
     CDS             complement(5438..5785)
                     /locus_tag="VXN30_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1357717.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5790..6905)
                     /locus_tag="VXN30_001088"
     CDS             complement(5790..6905)
                     /locus_tag="VXN30_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1357718.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6915..7874)
                     /locus_tag="VXN30_001089"
     CDS             complement(6915..7874)
                     /locus_tag="VXN30_001089"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1357719.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7987..8556)
                     /locus_tag="VXN30_001090"
     CDS             complement(7987..8556)
                     /locus_tag="VXN30_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1357720.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8684..9355
                     /locus_tag="VXN30_001091"
     CDS             8684..9355
                     /locus_tag="VXN30_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1357721.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            9339..10157
                     /locus_tag="VXN30_001092"
     CDS             9339..10157
                     /locus_tag="VXN30_001092"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1357722.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            10154..11050
                     /locus_tag="VXN30_001093"
     CDS             10154..11050
                     /locus_tag="VXN30_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1357723.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            11094..12068
                     /gene="pta"
                     /locus_tag="VXN30_001094"
     CDS             11094..12068
                     /gene="pta"
                     /locus_tag="VXN30_001094"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1357724.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3607 a         2356 c         2646 g         3679 t
ORIGIN      
        1 ttttggtatc cacgaaagca acttaatccg tcggagctaa tgggttgaag taactcttgt
       61 tcaaagtggt gttacgattt caagaactcc tctcagctct gaggccacgg taatgattga
      121 tgcgacggaa gtaaaaatca atcgccctaa aaaaagaatt agcgaattat tcttgtaaaa
      181 aaatgccacg ctatgaaggc tcaagcgatt gtcacaagtc aagggagaat tgtttctttg
      241 gatatcactg tgaactattg tcatgatatg aagttgttca aaatgagttg cagaaatatc
      301 ggacaagctg gtaaaatctt ggctgacagt ggttatcaag ggctcatgaa gatatatcct
      361 caagcacaaa ctccacgtaa atccagcaaa ctcaagccgc taacagttga agataaagcc
      421 tgtaaccatg cgctatccaa ggagagaagc aaggttgaga acatctttgc caaagtaaaa
      481 acgtttaaaa taatttcaac aacctatcga aatcatcgta aacgcttcgg attacgaatg
      541 aatttgagtg ctggtattat caatcatgaa ctaggattct agttttgcag gaagtctatt
      601 gaggtattga gctagtttat gaaaaaattg ggtgaaaagt cgagtgtttt agaaacccac
      661 agtgtagtat tctagtttca atccactata ttttgctact ccccgtaaag tttctatttt
      721 ccctgatttc tgatataata gaaatattga cttcaagagt aaggaagaga agatgaacgc
      781 attattaaat ggaatgaatg accgtcaggc tgaggcggtg caaacgacag aaggtccctt
      841 gctaatcatg gcaggggctg gttctggaaa gactcgtgtt ttaacccacc gtatcgctta
      901 tttgattgat gaaaagctgg tcaatccttg gaatatcttg gccattacct ttaccaacaa
      961 ggctgcgcgt gagatgaaag agcgtgctta tagcctcaat ccagctactc aggactgtct
     1021 gattgcgacc ttccactcca tgtgtgtgcg tattttgcgt cgcgatgcgg accatattgg
     1081 ctacaatcgt aattttacaa ttgtggatcc tggtgaacag cgaacgctca tgaaacgtat
     1141 tctaaaacag ttgaacttgg atcctaaaaa atggaatgaa cgaactattt tggggaccat
     1201 ttccaatgct aagaatgatt tgattgatga tgttgcttat gctgcccaag ctggcgatat
     1261 gtatacgcaa attgtggccc agtgttatac agcctatcaa aaagaacttc gtcagtccga
     1321 atccgttgac tttgatgatt tgattatgct gaccttgcgt ctctttgatc aaaatcctga
     1381 tgttttgacc tactaccagc aaaaattcca atacatccac gttgatgagt accaagatac
     1441 caaccatgct cagtaccaat tggtcaaact cttggcttcc cgttttaaaa atatctgtgt
     1501 ggttggggat gcggaccagt ctatctacgg ttggcgtggt gctgatatgc agaatatctt
     1561 ggactttgaa aaggattacc ccaaagccaa ggttgttttg ctggaggaaa attaccgctc
     1621 aaccaaaacc attctccaag cggccaacga ggttattaaa aataataaaa atcgccgtcc
     1681 taaaaatctc tggactcaaa acgctgatgg ggagcaaatc gtttactatc gtgccgatga
     1741 tgagctggat gaggctgtat ttgtagccag aaccatcgat gaacttagtc gcagtcaaaa
     1801 cttccttcat aaggattttg cagttctcta tcggactaat gcccagtccc gtacaattga
     1861 ggaagccctg ctcaagtcta acattcctta taccatggtt ggtggaacca aattctacag
     1921 ccgtaaggaa attcgcgata ttattgctta tctcaacctt attgctaatt tgagtgacaa
     1981 tattagtttt gagcgtatta tcaacgagcc taaacgtgga attggtctag gtacagttga
     2041 gaaaatccgt gattttgcaa atttgcaaaa tatgtctatg ctggatgctt ctgctaatat
     2101 tatgttgtct ggtatcaagg gtaaggcagc ccaatctatc tgggattttg ccaatatgat
     2161 gcttgatttg cgggagcagc tagaccactt aagcattaca gagttggttg agtccgtcct
     2221 agaaaaaaca ggttatgtcg atattcttaa cgcccaagcg actctagaaa gcaaggcacg
     2281 ggttgaaaat atcgaagagt ttctttctgt tacgaagaac tttgatgaca ccacggatgt
     2341 gacagaagag gaaactggtc tggacaaact gagtcgtttc ttaaatgact tggctttgat
     2401 tgtcgacaca gattcaggta gtcaggagac atcagaagtg accttgatga ccctgcatgc
     2461 tgccaaaggt ctcgaatttc cagttgtctt tttgattggg atggaagaaa atgtctttcc
     2521 acttagtcgt gcgactgaag atccagatga attagaagaa gagcgccgtc tagcctatgt
     2581 aggtatcacg cgtgcagaga aaattctcta tctgaccaat gccaactcac gcttgctttt
     2641 tggtcgtacc aattataacc gtccgactcg ttttattaac gaaatcagtt cagacttgct
     2701 tgagtatcaa ggtctggctc gtcctgcaaa tacaagcttt aaggcatcat atagcagtgg
     2761 tagtatttcc tttggtcaag gtatgagttt ggctcaggct cttcaagacc gtaaacgcgt
     2821 tgctgcccca aaatcaatcc agtcaagcgg tcttccattt ggtcaattta cagctggcgc
     2881 aaaaccagca tctagcgagg caaattggtc cattggtgat attgctctcc acaagaaatg
     2941 gggagaggga accgttctgg aagtttcagg tagcggtgct aggcaggaat tgaaaatcaa
     3001 tttcccagaa gtaggtttga aaaaactttt agccagtgtg gctccaattg agaaaaaaat
     3061 ctaattttcc atccttctca cgaataataa agtgaggagg atttttatgt acagtatttc
     3121 attccaagaa gattcactat taccaagaga aaggctggcc aaggaaggag ttgaagcgct
     3181 tagtaaccaa gagttgctag ctattttact caggacagga acacgtcaag ctagcgtttt
     3241 tgaaattgcc caaaaagtct tgaacaatct ttcaagccta acggatttga aaaaaatgac
     3301 cctgcaggaa ttgcagagtt tgtctggtat tgggcgtgtt aaggccatag aattacaagc
     3361 tatgattgaa ctggggcatc gtattcacaa acacgagact cttgaaatgg aaagtattct
     3421 cagcagtcaa aagttggcca agaagatgca gcaggaatta ggggataaaa aacaagagca
     3481 cctggtggca ctctatctca atactcaaaa tcaaatcatc catcagcaga ccatttttat
     3541 cggttctgta actcgtagta tcgctgaacc gcgagagatt ctgcactatg caatcaagca
     3601 tatggcgact tctcttatct tggtccacaa tcatccttca ggagcggtag cgcctagcca
     3661 aaatgatgat catgtcacta aacttgttaa agaagcctgc gaactgatgg ggattgttct
     3721 cttggaccat ttgattgtct ctcattctaa ttactttagt tatcgtgaaa agacagattt
     3781 aatctaaagt tcattaacga catagtcaaa gagtttctta tctttgggac gattttcaaa
     3841 aagaagttct ggatgccatt ggacaccgag aaaggcgaca tcatccgtac tcatgacagc
     3901 ctcaatgata ccatctttag gatcatgagc cacaactttt aaatttggtg ctaagtcctt
     3961 gatgctctgg tggtggaagg agttgatatg agagatttct ccatagattt cttggagaac
     4021 ggtatctggt tctgttacca agcgttgagt tgtgtactca acagaagaat cctgccaatg
     4081 gtcttcgata tcttggtaca aagttccacc catggcaacg ttaaagagtt gggtaccacg
     4141 gcagacagag aaaatgggct ttttctgttt aatagcttcc ttgatgaggg ccagttcgaa
     4201 gatatctctt tgaaggtgat aatcatcgct atcaatggtt ttgggttcgc catagaattt
     4261 tggatcgaca ttttgcccac ctgtcaagat gagcttgtca atcaaactga tatagtggca
     4321 ggccatttct tgatcaccaa tcggtaggat gataggaatc cctccagcat ctttaacgcc
     4381 ttcaacaaag ccttttgctg cgtagctcat catgatgtca tcatctggat gagttttttc
     4441 gtttcctgta atcccaataa ctggtttttt cataaaatga ttttcgcttt ctaatcctct
     4501 tttcgcatga agtagaggag ggtttggagt tcacttgtca aatcgacata ctgaacgacc
     4561 acgtcttttg gtaaatgcag atggactggt gaaaaactga gaattccttt cacaccagca
     4621 tcaaccaaga gattagcaac ctcttgtgac ttgacgctgg gaacagttag gatagcagtc
     4681 ttcacatcag catccttgat tttgtccttg atctgagaaa tcccgtaaat gggaatcccg
     4741 tcaggagttt gggtaccgac ttcaggatgg tcgtctaggt caaaggccat gataatcttc
     4801 atcttgttac gttcgtggaa gcggtagtgg agaagggcat ggcccatatt tccaatacca
     4861 accagcatga cattggtaat agagttgtca ttgagcaaat cggcaaaaaa tgtcattagt
     4921 tttttgacat catagccaaa accacgacga ccaagttcac caaaatagga aaaatcacga
     4981 cgtacggtcg ctgaatcaat accgatagcc tctgcaattt gcttagagtt ggcacgttca
     5041 atcttttctg catgaaatct cttaaaaatt cgatagtaga gagagagtct ttttgctgta
     5101 gcttttggaa tagcaaactg tttatctttc acaaaatcac aacctttcta ttcttctatt
     5161 ttatagaaac attgtgaaaa aatcaacaaa aataagaaaa aactaagaaa aatcttagtt
     5221 ttgatgtaaa aaatctgcat gagatagaaa acggtagagg tctccgacca gaccctgata
     5281 aactttttgg cccctaaaag tcagagaagt cacataaagt gtatctggta aggttacaca
     5341 tcctgacaaa gtcaacatga gagcctcatg atcctcatac ttgagagtac gctctacatg
     5401 atagcagtcc ttataggtca gttcaaacat tttggctcta tctttccgat tttgtaaaga
     5461 caccacgttc taccaagcta tccatgagga agtagaattt ttcctgatga atatggtggt
     5521 cttctgattt gaaaatatca actagacgaa ggccaaactt gtcagtgata ttgattttag
     5581 cccctgtaag ttccttgtta atgatgattt tgagttggaa gccttcaccg ctgtttggca
     5641 ctttttccaa aaggcgagtc agttcatagt taccaacctt agtttcaaaa aaggtgttat
     5701 ctttgagggt gaatttttta acagaagggc taagagtgta atcgtaacga caatttttta
     5761 actgaatgat tttttcaaat gccatatggc taacctccga taatttcttt taaggttttt
     5821 gcgagggttt gtaggtcttc aacggtattt tgtggcgaca aactgatgcg aagggattcc
     5881 ttcaagcgtt ctgaatttgc gccatacatg gcttcaagaa catggctgga ttggacaacg
     5941 cctgcagtac aggctgagcc agtagagatt gaaattccag ctaaatctag ccgaaggagt
     6001 aagaggtcat ttttctgacc aggaaatcca atattgagaa cataagggag atgatgtttt
     6061 cctctattca agtaatactg aatgccctcc agctctgcca gaaaggcagt ttctagattt
     6121 tgtacatgtt gaaaatgttc ttcttgtttt tctaggtctt cttttagggc tgcaaccatg
     6181 cctacgatag caggcagatt ttcagttcct gcacgttttt tctgttcctg gtctccgcca
     6241 tgtagatagg aatcaaagtc catgctagat gcgtagagaa aaccgattcc cttaggacca
     6301 tggaatttgt gggcagaagc agtgagaaaa tcaatgccca attcttctga atgaattggg
     6361 attttaccaa tagcctgaac tgcatcaaca tgataggcag cagggtgttg cttgagtatt
     6421 tggccaattt cagcgatggg cagtaggttt cctgtctcat tattggcaaa catggtagaa
     6481 accaaaatcg tatcgtcacg taaagccttt tgaatttgct gggctgtgat ttcttgattt
     6541 tctggctgga taatggttgc ttcaaaccca aagtgttgaa ccaagtaatc aattgtttca
     6601 aggacagcat ggtgctcgat ggcagttgtg atgatatgtt ttccttgttc ttggtgacga
     6661 agacagtagc caatgatggt agtattattg ccttcagtcc caccagaagt gaaaaagata
     6721 tgttgaggtt ttgtccttag taactgggct agttcctgac gggcttctcg caagagtttg
     6781 ccagcttgac gaccatgacc atgaatacta gaaggatttc cgtgggtttc ttgcataacc
     6841 ttggtcatag ctgaaatagc aactgctgac ataggagtcg ttgcagcatt gtccaaataa
     6901 atcaaagaat caccttattt ctttttattg taggcaaaga gtgggctgac tggttttctt
     6961 tcgtgaatac ggacgatagc atcaccaatt aactcactag cagtgatgta gcatacattt
     7021 ttaggagttt tttcttttgt tgctactgaa tcagtcacaa gaatttcttt aatattagta
     7081 ttgtcaagaa gctcagcagc tccctcgacg aagagaccgt ggctagaaac agcataaatt
     7141 tctgtagctc cttcacgttc aacgatttta gaagcttcag agaaggtacg tcctgtattt
     7201 aaaatatcat caatcaagat agctttctta ccttcaacat caccaataat ataaccttcg
     7261 ttacgagttg catcgtcttg agggtagtcg ataatggcga taggagcatc aagatattca
     7321 gccaggctac gcgcacgttt gacacctgaa tttttagggc taacgacaac aacatctgaa
     7381 ccaagcaatc ctttatcgca gtaatgtttt gcgaataggg gaacagtgaa aagattatcc
     7441 actggaatat caaagaaacc ttgaacctga acggcatgca aatcaagagt caggatacga
     7501 tcaactccag ccttaaccag catattggca actagttttg ctgtaagtgg ctcacgagga
     7561 caagcaatgc ggtcttgacg tgcatagcca aaatatggaa ggacaacgtt gatactgtgg
     7621 gcacttgcac gcacacaagc atcgaccatg attaacaatt ccattaggtg gttgttgaca
     7681 gggaaacttg ttgattggat gatgtaaaca tcataaccac ggacactttc ttcgatattt
     7741 acttggattt ctccgtctga aaattgacgt gatgatagtt ttccaagtgg gacaccaaca
     7801 gcttgggcaa ttttttgtgc aatctcttgg ttagagttga gtgcgaaaag tttcatgttt
     7861 tttctatctg acattataga ccgtcctctg taaactttat aaatcctagt tatatttacc
     7921 ttacatatat gaactgggat ttgtgtattt ttatcttttc tattttacca aaaaatggag
     7981 attatttcag ctatttttca tacttttgac aaatcgaacc aattttgaag gagctttttg
     8041 gtaggaaatc tgatttttct ctaaaaattg tcggaaatcc tgtttgcctt gctcatgatt
     8101 ttccacttca agctccaatt cgtaatctgt tatatcaaag tatcggctct gatccagtgc
     8161 catgagacca atagctgttt tcatttcata gcgaagcgtt gttagacaac caagaacctg
     8221 ccagttctta ctttggatac catgtttcgc caattcatcc agtactagcc cttgaggaag
     8281 ttcttcctta ctcagatagt tctcagcatc ttttagttgc aatttttggt tgtattccat
     8341 gtttccaaca ctctgcggga ctttgagtgt caactcagcc cagtcttcaa aggttcgaat
     8401 gcgcatagcg actttctttt ctcgcagttc aaaatcaggc gtgtcgatgt agtaatttgt
     8461 ttgaagaaca ggagtgacac ctgtgaactg gtcttttaga cgattgtatt catctttttt
     8521 caatagtgtt ttcaattcaa tttctaaatg tttcattttt cttacctttt tttatcgttg
     8581 aaagcggatt tatggtataa taagcattgt atttattgta tatgaatctg gagaaaaaat
     8641 caaagatatt tttgacggat aatatgagaa caagggagaa tatatgacct tagaatggga
     8701 agaatttcta gatccttaca ttcaagctgt tggtgagtta aagattaaac ttcgtggtat
     8761 tcgtaagcaa tatcgtaagc aaaataagca ttctccaatt gagtttgtga ccggtcgagt
     8821 taagccaatt gagagcatca aagaaaaaat ggctcgtcgt ggcattactt atgcgacctt
     8881 ggaacacgat ttgcaggata ttgctggctt acgtgtgatg gttcagtttg tagatgacgt
     8941 caaggaagta gtggatattt tgcacaagcg tcaggatatg cgaatcatac aggagcgaga
     9001 ttacattact catagaaaag catcaggcta tcgttcctat catgtggtag tagaatatac
     9061 ggttgatacc atcaatggag ttaagactat tttggcagaa attcaaattc gtactttggc
     9121 catgaatttc tgggcaacga tagaacattc tctcaactac aagtaccaag gggatttccc
     9181 agatgagatt aagaagcgac tggaaattac agctagaatc gcccatcagt tggatgaaga
     9241 aatgggtgaa attcgtgatg atatccaaga agctcaggca ctttttgatc ctttgagtag
     9301 aaaattaaat gacggtgtag gaaacagtga cgatacagat gaagaataca ggtaaacgaa
     9361 ttgatctgat agccaataga aaaccgcaga gtcaaagggt tttgtatgaa ttgcgagatc
     9421 gtttgaagag aaatcagttt atactcaatg ataccaatcc ggatattgtc atttccattg
     9481 gcggggatgg tatgctcttg tcggcctttc ataagtacga aaatcagctt gagaaggtcc
     9541 gctttatcgg tcttcacact ggacatttgg gcttctatac agattatcgt gattttgagt
     9601 tggacaagct agtgactaat ttgcagctag atactggggc aagggtttct taccctgttc
     9661 tgaatgtgaa ggtctttctt gaaaatggtg aagttaagat tttcagagca ctcaacgaag
     9721 ccagcatccg caggtctgat cgaaccatgg tggcagatat tgtaataaat ggtgttccct
     9781 ttgaacgttt tcgtggagac gggctaacag tttcgacacc gactggtagt actgcctata
     9841 acaagtctct tggcggtgct gttttacacc ctaccattga agctttgcaa ttaacggaaa
     9901 ttgccagcct taataatcgt gtctatcgaa cactgggctc ttccattatt gtgcctaaga
     9961 aggataagat tgaacttatt ccaacaagaa acgattatca tactatttcg gttgacaata
    10021 gcgtttattc tttccgtaat attgagcgta ttgagtatca aatcgcccat cataagattc
    10081 actttgtcgc gactcctagc cataccagtt tctggaaccg tgttaaggat gcctttatcg
    10141 gtgaggtgga tgaatgaggt ttgaatttat cgcagatgaa catgtcaagg ttaagacctt
    10201 cttaaaaaag cacgaggttt ctaagggatt gctggccaag attaagtttc gaggtggagc
    10261 tattctggtc aataatcaac cgcaaaatgc aacgtatcta ttggacgttg gagactacgt
    10321 taccattgac attcccgctg agaaaggctt tgaaaccttg gaggctattg agcttccatt
    10381 agatattctc tatgaggatg accactttct agtcttgaat aaaccctatg gagtggcttc
    10441 tattcctagt gttaatcact ctaataccat tgccaatttt atcaagggtt actatgtcaa
    10501 gcaaaattat gaaaatcagc aggttcacat tgttaccaga ctagataggg acacttctgg
    10561 cttgatgctc tttgccaagc acggttatgc ccatgcacga ttagacaagc agttgcagaa
    10621 gaaatctatc gagaaacgct actttgcttt ggttaaggga gatggacatt tggaaccaga
    10681 aggggaaatt attgctccga ttgcgcgtga tgaagattcc attattacca gacgagtggc
    10741 taaaggcgga aagtatgccc atacttcata caagattgta gcttcttatg gaaatattca
    10801 cttggtctat attcacctgc acactggtcg aacccatcaa atccgagtcc atttttctca
    10861 tatcggtttt cctttgctgg gagatgattt gtatggtggt agtctggaag atggtattca
    10921 acgtcaggct ctgcattgcc attacctatc cttttatcat ccatttttag agcaagactt
    10981 gcagttagaa agtcccttgc cggatgattt cagtaacctt attacccagt tatcaactaa
    11041 tactctataa aaactgtctc agagtataat tattatctta aaggagaaaa ctcatggaag
    11101 tttttgaaag tctcaaagcc aaccttgttg gtaaaaatgc tcgtatcgtt ctccctgaag
    11161 gggaagagcc tcgtattctt caagcaacaa aacgcttagt aaaagaaaca gaagtgattc
    11221 ctgttttgct tggaaatcct gaaaaaatta aaatttatct tgaaattgaa ggaatcatgg
    11281 atggttatga ggtcatcgac cctcaacatt atcctcaatt tgaagaaatg gtttctgcct
    11341 tggtggagcg tcgcaagggc aaaatgactg aagaagatgt acgcaaggtt ttggttgaag
    11401 atgtcaacta ctttggtgtg atgttggttt acttgggctt ggttgatgga atggtgtcag
    11461 gagcgattca ctcaacagct tcaacagttc gcccagctct acaaatcatc aaaactcgtc
    11521 caaatgtaac tcgtacttca ggagccttcc tcatggttcg tggtacggaa cgttacctat
    11581 ttggagactg tgccattaat atcaatccag atgcagaagc cttggctgaa attgccatca
    11641 actcagcaat cacagctaag atgtttggca tcgaacctaa aattgccatg ttgagctatt
    11701 ctactaaagg ttcagggttt ggtgaaagcg ttgataaggt cgttgaagca actaaaattg
    11761 ctcacgactt gcgtcctgac cttgaaatcg atggtgagtt gcaatttgat gcggcctttg
    11821 ttcccgaaac tgcagctctg aaagctccgg gaagtacagt agctggtcaa gcaaatgtct
    11881 tcatcttccc aggtatcgag gcaggaaata tcggttacaa gatggctgaa cgcctgggtg
    11941 gctttgcggc tgtaggacct gttttgcaag gtttaaacaa gccagttaat gatctttctc
    12001 gtggatgtaa tgcagatgat gtttacaagt tgaccctcat cacagcagct caagcagttc
    12061 atcaatagtg aaaactataa agtgatatac tatgctatac tgtagttatg aaactatgta
    12121 cgaaaagcac tgccattaat tcctgagaac taaattactg attggtgtca aaaaggaaaa
    12181 cttccaagcg atgatatcct gtctatacac gacctataga aatctgtaat atacatatcc
    12241 gtaaaacgat aaattccctt tttgatttta aatgagtatg aaaagaga
//
LOCUS       DAVLSL010000051        12252 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLSL010000051 DAVLSL010000000
VERSION     DAVLSL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12252
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.052"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            229..900
                     /locus_tag="VXN30_001095"
     CDS             229..900
                     /locus_tag="VXN30_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1357725.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(952..1149)
                     /locus_tag="VXN30_001096"
                     /pseudo
     CDS             complement(952..1149)
                     /locus_tag="VXN30_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1142..2050
                     /locus_tag="VXN30_001097"
     CDS             1142..2050
                     /locus_tag="VXN30_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1357726.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2047..2508
                     /locus_tag="VXN30_001098"
     CDS             2047..2508
                     /locus_tag="VXN30_001098"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1357727.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAILKEEINGN"
     gene            2498..3385
                     /locus_tag="VXN30_001099"
     CDS             2498..3385
                     /locus_tag="VXN30_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1357728.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3388..5271
                     /gene="cbpE"
                     /locus_tag="VXN30_001100"
                     /gene_synonym="pce"
     CDS             3388..5271
                     /gene="cbpE"
                     /locus_tag="VXN30_001100"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1357729.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            5351..6481
                     /locus_tag="VXN30_001101"
     CDS             5351..6481
                     /locus_tag="VXN30_001101"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1357730.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6491..7753
                     /locus_tag="VXN30_001102"
     CDS             6491..7753
                     /locus_tag="VXN30_001102"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1357731.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7757..8554
                     /gene="proC"
                     /locus_tag="VXN30_001103"
     CDS             7757..8554
                     /gene="proC"
                     /locus_tag="VXN30_001103"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1357732.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            8774..9412
                     /locus_tag="VXN30_001104"
     CDS             8774..9412
                     /locus_tag="VXN30_001104"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1357733.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILKEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9409..10299
                     /locus_tag="VXN30_001105"
     CDS             9409..10299
                     /locus_tag="VXN30_001105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1357734.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10339..10656
                     /gene="yabA"
                     /locus_tag="VXN30_001106"
     CDS             10339..10656
                     /gene="yabA"
                     /locus_tag="VXN30_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1357735.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10659..11528
                     /gene="rsmI"
                     /locus_tag="VXN30_001107"
     CDS             10659..11528
                     /gene="rsmI"
                     /locus_tag="VXN30_001107"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1357736.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKGEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11755..>12252
                     /locus_tag="VXN30_001108"
     CDS             11755..>12252
                     /locus_tag="VXN30_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1357737.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRK"
BASE COUNT         3671 a         2104 c         2909 g         3568 t
ORIGIN      
        1 gctgatacaa ggtggagcag attcatatgg taaaaatcct aatggtaggt acgattggga
       61 aatagaacca gtattaactc tgccggttca tggattttgt cccagaggca cctatgattt
      121 aggatatatt ggaggaggca atcatctttg caaaggaagt gctgcgagat tttaagtaaa
      181 atttattagt aatatgaaga aacaagtgga gaaagcagag aatttaatat gaaaaaacgg
      241 gctattcaaa ttttactagc attgtcctta attttttaca aatcaacttg gttttggaga
      301 gttttcaatc acctcgcaaa gccctatcta ccagcaagtc gtgaattttt tcagattctg
      361 cttttgatgg agagcggagt tcttttctta gcggtcatct atctactggt ttttgcagga
      421 aagaaaattt ttcatttcaa gtggcagctg aggtacttca tctacctttt actgggctac
      481 atcatttcat atatgtctga cttcctcttt tcgtatttca tatccctgtc ttcaaatcag
      541 atttctttga atgaaacggt agaaatgatg gggagacagg agttccctta tttcttgctc
      601 atcgtttgct tcatcgcccc tattgctgag gaattgattt atcgaggtgt gcttatgaca
      661 acctttttca aaaactcacc ttggtacgga gatgttttgc tttctgctat tattttcggt
      721 tatattcata tcaattttgc tttaactcct cttgcttttt tcatttatgc tagtggaggt
      781 cttattttag ctctattgta tcgcatgact aaaaatctct actatccaat actagttcat
      841 attctcatta atatcactgc cttctgggat gtgtggttgc tcctattttc aggaagttag
      901 cttactaaaa taatgtcgga actttccggc attttctttt ttcacaaata gtcaacggtt
      961 ttcttttcga tattgtagtg gtgtgtatcc agttattttt ttgaattgat tttgaaaata
     1021 aggttggctt gagaaaggca gatagtgaag atagttaaga agaagaggat gttctttttt
     1081 cctttttgga aaacttctaa aatatggtat aatgaaaaga taaagaagtt aggggtagaa
     1141 gatgaacatt caacaattac gctatgttgt ggccattgcc aatagtggta ctttccgtga
     1201 agctgctgaa aagatgtatg ttagtcagcc gagtctgtct atttctgttc gtgatttgga
     1261 aaaagagttg ggttttaaga ttttccgtcg gaccagctca gggactttct tgacccgtcg
     1321 tgggatggaa ttttatgaaa aagcgcaaga attggttaaa ggatttgata tttttcaaaa
     1381 tcagtatgcc aatcctgaag aagaaaaaga tgaattttct gttgctagcc agcactatga
     1441 cttcttgcca ccaactatta cggccttttc agagcgctat cctgactata agaacttccg
     1501 tatttttgaa tcaactactg ttcaaatatt agatgaagtg gcgcaagggc atagtgagat
     1561 tgggattatc tacctcaaca atcaaaataa aaaggggatt atgcaacggg ttgaaaaatt
     1621 aggtctggag gtcatcgaat tgattccttt ccatacccat atttatctcc gtgagggtca
     1681 tcctttagcc cagaaagagg aattagtcat ggaggattta gcggatttac caacggttcg
     1741 tttcactcaa gagaaagacg agtaccttta ttattcagag aactttgtcg ataccagcgc
     1801 tagctcacag atgtttaatg tgacagaccg tgccaccttg aatggtattt tggagcggac
     1861 ggacgcctat gcgacaggtt ctggattttt agatagtgac agtgttaatg gcattacagt
     1921 tattcgtctc aaggataacc tagataaccg catggtctat gttaaacgtg aagaagtgga
     1981 gcttagtcaa gctgggactc tcttcgtaga agtcatgcaa gaatattttg atcaaaagag
     2041 gaaatcatga aaaaaagagc aatagtggca gtcattgtac tgcttttaat tgggctggat
     2101 cagttggtca aatcctatat cgtccagcag attccactgg gtgaagtgcg ctcctggatt
     2161 cccaatttcg ttagcttgac ctacctgcaa aatcgaggtg cagccttttc tatcttacaa
     2221 gatcagcagc tgttattcgc tgtcattact ctggttgtcg tgataggtgc catttggtat
     2281 ttacataaac acatggagga ctcattctgg atggtcttgg gtttgactct aataatcgcg
     2341 ggtggtcttg gaaactttat tgacagggtc agtcagggct ttgttgtgga tatgttccac
     2401 cttgacttta tcaactttgc aattttcaat gtggcagata gctatctgac ggttggagtg
     2461 attattttat tgattgcaat actaaaagag gaaataaatg gaaattaaaa ttgaaactgg
     2521 tggtctgcgt ttggataagg ctttgtcaga tttgtcagaa ttatcacgta gtctcgcgaa
     2581 tgaacaaatt aaatcaggcc aggtcttggt caatggtcaa gtcaagaaag ctaaatacac
     2641 agtccaagag ggtgatgtcg tcacttacca tgtgccagaa ccagaggtat tagagtatgt
     2701 ggctgaggat cttccgctag aaatagtcta ccaagatgag gatgtggctg tcgttaacaa
     2761 acctcaggga atggttgtgc acccgagtgc tggtcatacc agtggaaccc tagtaaatgc
     2821 cctcatgtat catattaagg acttgtcggg tatcaatggg gttctgcgtc cagggattgt
     2881 tcaccgtatt gataaggata cgtcaggtct tctcatgatt gctaaaaacg atgatgcgca
     2941 tctagtactt gcccaagaac tcaaagataa aaagtctctc cgcaaatatt gggcgattgt
     3001 tcatggaaat ctgcctaatg atcgtggtgt aattgaagcg ccgattggcc ggagtgaaaa
     3061 agaccgtaag aaacaggctg taactgctaa agggaagcct gcagtgacgc gttttcacgt
     3121 cttggaacgc tttggcgatt atagcttagt agagttgcaa ctggagacag ggcgcactca
     3181 tcaaatccgt gtccacatgg cttatatcgg ccattcagtc gctggtgatg aggtctatgg
     3241 tcctcgcaag actttgaaag gacatggaca atttcttcat gccaagactt taggttttac
     3301 tcatccgaga acaggtaaga ccttggaatt taaagcagat atcccagaga tttttaagga
     3361 aaccttggag agattgagaa agtaagaatg aaaaagaaat taactagttt agcacttgta
     3421 ggcgcttttt taggtttgtc atggtatggg aatgttcagg ctcaagaaag ttcaggaaat
     3481 aaaatccact ttatcaatgt tcaagaaggt ggcagtgatg cgattattct tgaaagcaat
     3541 ggacattttg ccatggtgga tacaggagaa gattatgatt tcccagatgg aagtgattct
     3601 cgctatccat ggagagaagg aattgaaacg tcttataagc atgttctaac agaccgtgtc
     3661 tttcgtcgtt tgaaggaatt gggtgtccaa aaacttgatt ttattttggt gacccatacc
     3721 cacagtgatc atattggaaa tgttgatgaa ttactgtcta cctatccagt tgaccgagtc
     3781 tatcttaaga aatatagtga tagtcgtatt actaattctg aacgtctatg ggataatctg
     3841 tatggctatg ataaggtttt acagactgct gcagaaaaag gtgtttcagt tattcaaaat
     3901 atcacacaag gggatgctca ttttcagttt ggggacatgg atattcagct ctataattat
     3961 gaaaatgaaa ctgattcatc gggtgaatta aagaaaattt gggatgacaa ttccaattcc
     4021 ttgattagcg tggtgaaagt caatggcaag aaaatttacc ttgggggcga tttagataat
     4081 gttcatggag cagaagacaa gtatggtcct ctcattggaa aagttgattt gatgaagttt
     4141 aatcatcacc atgataccaa caaatcaaat accaaggatt tcattaaaaa tttgagtccg
     4201 agtttgattg ttcaaacttc ggatagtcta ccttggaaaa atggtgttga tagtgagtat
     4261 gttaattggc tcaaagaacg aggaattgag agaatcaatg cagccagcaa agactatgat
     4321 gcaacagttt ttgatattcg acaagacggt ttagtaaaca tttcaacttc ctacaagccg
     4381 attccaagtt ttcaagctgg ttggcataag agttcatatg gaaactggtg gtatcaagcg
     4441 cctgattcta caggagaata tgctgtcggt tggaatgaaa ttaagggtga atggtattac
     4501 tttaaccaaa cgggtatctt attacagaat caatggaaaa aatggaacaa tcgttggttc
     4561 tatttgacag actctggtgt tgctgctaaa aattggaaga aaatcgctgg aatctggtat
     4621 tattttaaca aagaaaacca gatggaaatt ggttggattc aagataaaga gcagtggtat
     4681 tatttggatg ttgatggctc tatgaagaca ggttggcttc aatataaggg gcaatggtat
     4741 tactttgctc catcagggga aatgaaaacg ggctgggtaa aagataaaga agcctggtac
     4801 tatatggatt ctactggtat catgaagaca ggtgagatag aagttgctgg acatcattac
     4861 tatctagaag aatcaggagc tatgaagcaa ggctggctta aaaaggcaaa tgattggtat
     4921 ttctacaaga cagatggttc acgagctata ggttggatta aagacaagga taaatggtac
     4981 ttcttgaaag aaaatggtca attacttgtg aatggtaaga caccagaagg ctatactgtg
     5041 gattcaagtg gtgcctggtt agtggatgtt ccgatcgaga aatctgctac aattaaaact
     5101 acaagtcatt cagaaataaa agaatccaaa gaagtagtga aaaaggatct tgaaaataaa
     5161 gaaacgagtc aacatgaaag tgttacaaat tcttcaacta gtcaagattt gacatcctca
     5221 acttcacaaa gctctgaaac gagtgtaaac aaatcggaat cagaacagta gtagaaaaga
     5281 aggttttagg gccttctttt tcctatcaac tcttttctat ttcctgttat tcatgttata
     5341 atggataaat atgaataatc ggagtgagac tatgaaatac aaacggattg tctttaaggt
     5401 gggtacttct tctctgacaa atgaggatgg aagtttatca cgtagtaagg taaaggatat
     5461 tacccagcag ttggctatgc tgcacgaggc tggtcatgag ttgattttgg tgtcttcagg
     5521 tgccattgcg gctggttttg gagccttagg atttaaaaag cgtccgacta aaattgctga
     5581 taaacaggct tcagcagcgg tagggcaggg acttttgttg gaagaatata cgaccaatct
     5641 tctcttgcgt caaatcgttt ctgcacaaat cttgctgacc caagatgact ttgtggataa
     5701 gcgtcgttat aaaaatgccc atcaggcttt gtcggttttg ctcaaccgtg gggcaattcc
     5761 tatcatcaat gagaatgata gtgtcgttat tgatgagctc aaggttgggg acaatgacac
     5821 tctaagtgct caagtagcgg cgatggtcca agcagacctt ttagttttct tgacagatgt
     5881 ggacggtctc tatactggaa atcctaattc agatccaaga gccaaacgct tggagagaat
     5941 cgagaccatc aatcgtgaga ttattgatat ggctggtgga gctggttcgt caaacggaac
     6001 tgggggtatg ttaaccaaaa tcaaggctgc aactatcgcg acggaatcag gagttcctgt
     6061 ttatatctgc tcatccttga aatcagattc catgattgag gcggcagagg agaccgagga
     6121 tggttcttac tttgttgctc aagagaaggg gcttcgtacc cagaaacaat ggcttgcctt
     6181 ctatgctcag agtcaaggtt ctatttgggt tgataaaggg gctgcggaag ctctctctca
     6241 acatggaaag agtcttctct tatctggtat cgttgaagca gaaggagtct tttcttacgg
     6301 tgatatcgtg acagtatttg ataaggaaag tggaaaatca cttggaaaag gacgcgtgca
     6361 atttggagca tctgctttgg aggatatgtt gcgttctcaa aaagccaagg gtgtcttgat
     6421 ttaccgtgac gactggattt ccattactcc tgaaatccaa ctacttttta cagaatttta
     6481 gaggtaaact atggtgagta gacaagaaca atttgaacag gtacaggctg ttaaaaaatc
     6541 gattaacaca gctagtgaag aagtgaaaaa ccaagccttg ctagccatgg ctgatcactt
     6601 agtggctgct actgaggaaa ttttagcggc taatgccctc gatatggcag cggctaaggg
     6661 gaaaatctca gatgtgatgt tggatcgtct ttatttggat gcagatcgta tagaagcgat
     6721 ggcaagagga attcgtgaag tggttgcctt accagatcca atcggtgaag ttttagaaac
     6781 aagtcagctt gaaaatggtt tggttatcac aaaaaaacgt gtagctatgg gtgtcatcgg
     6841 tattatctat gaaagccgtc caaatgtgac gtctgatgcg gctgctttga ctcttaagag
     6901 tggaaatgcg gttgttcttc gtagtggtaa ggatgcctat caaacaaccc atgccattgt
     6961 cacagccttg aagaagggct tggagacgac tactattcat ccaaatgtga ttcaactggt
     7021 ggaggatact agccgtgaaa gtagttatgc tatgatgaag gccaagggct atctagacct
     7081 tctcattcct cgtggaggag ctggcttgat caatgcagtg gttgagaatg cgattgtacc
     7141 tgttatcgag acagggactg ggattgtcca tgtctatgtg gataaggatg cagacgaaga
     7201 caaggcgctg tctatcatca acaatgctaa aaccagtcgt ccttctgttt gtaatgccat
     7261 ggaggttctg ctggttcatg aaaacaaggc agcaagcatc cttcctcgct tggatcaaat
     7321 gctggttgca gatcgaaaag aagctgggtt ggaaccaatt caattccgcc tagatagcaa
     7381 agcaagccag tttgtttcag gtcaagctgc tcaagcacaa gactttgata ccgagttttt
     7441 agactatatt ctagctgtta aggttgtgag cagtttagaa gaagcggttg cgcatattga
     7501 atcccacagt acccatcatt cggatgctat tgtgacggaa aatgctgaag ctgcagcata
     7561 ctttacagat caagtggact ctgcagcggt gtatgttaat gcctcaactc gtttcacaga
     7621 tggaggacaa tttggtcttg gttgtgaaat ggggatttct actcagaaat tgcacgcgcg
     7681 tggtccaatg ggcttgaaag agttgaccag ctacaagtat gtggttgctg gtgatgggca
     7741 gataagggag taagagatga agattggatt tatcggtttg gggaatatgg gtgctagctt
     7801 ggcaaaatct gtcttgcaga ctaggacgtc agatgagatt ctccttgcca atcgtagtca
     7861 agctaaggta gatgctttca ttgcagactt tggtggtcag gcttccagca atgaagaaat
     7921 gtttgcagaa gcagatgtga tttttctagg agttaagcct gctcagtttt ctgaactgct
     7981 ttctcaatat cagaccatcc ttgaaaaaag agaaagtctt cttttgattt cgatggcagc
     8041 tggattgacc ttagaaaaac tagcaagtct tatcccaagc caacaccgaa ttattcgtat
     8101 gatgcctaat acccctgctt ctatcgggca aggagtgatt agttatgcct tgtctcctaa
     8161 ttgcagggct gaggacagtg agatcttttg tcagctttta gccaaggctg gtctcttggt
     8221 tgaattagga gaaagcttaa tcaatgcagc gacaggtctt gcaggttgtg gaccagcctt
     8281 tgtctatctc tttattgagg ccttggcaga tgcaggtgtt cagacgggat taccacgaga
     8341 aacagccttg aaaatggcag cccaaactgt ggtaggagct gggcaattgg tcctagaaag
     8401 ccagcaacat cctggagttt tgaaagatca agtttgtagt ccaggcggtt cgactattgc
     8461 tggcgtagca agcctagaag cgcatgcttt tcgaggcaca gtcatggagg cagttcatca
     8521 agcctataaa cgaacacaag aactaggtaa ataagaggta gttttatctg cctcttttat
     8581 ggtggttgat actcttcgaa aatcaaattc aaactgcgtc agcttcacct tgccgtactc
     8641 aagtacagcc tgcggctagc ttcctagttt gctctttgat tttcattgag tatgaaatga
     8701 gaagacaaaa agattgtcac aaacccctat ttttttgata gaatagaagt agtaaaaaag
     8761 aaatgagtta gacatgtcaa aaggattttt agtctctctt gagggaccag agggagcagg
     8821 caagaccagt gttttagagg ctctgctacc aattttaaag gaaaaaggag tagaggtgtt
     8881 gacgacccgt gaacctggcg gagtcttgat tggggagaag attcgggaag tgattttgga
     8941 tccaagtcat actcagatgg atgctaaaac agagctactt ctctatattg ccagtcgcag
     9001 acagcatttg gtggaaaaag ttcttccagc ccttgaagct ggcaagttgg tcatcatgga
     9061 tcgttttatc gatagttctg ttgcctatca gggatttggt cgtggcttag atattgaagc
     9121 cattgactgg ctcaatcagt ttgcgacaga tggcctcaaa cccgatttga cactctattt
     9181 tgacatcgag gtggaagaag ggctggctcg tattgctgcg aatagtgacc gcgaggttaa
     9241 tcgtttggat ttggaagggt tggacttgca taaaaaagtt cgtcaaggct acctttctct
     9301 tctggataaa gagggaaatc gcattgtcaa gattgatgct agtctccctt tggagcaagt
     9361 tgtggaaact accaaggctg tcttgtttga cggaatgggc ttggccaaat gaaacaagat
     9421 caactaaagg cttggcaacc agctcagttt gaccgttttg tccgtatctt agaacaagac
     9481 cagctcaatc acgcctatct cttttcaggt ttctttggaa gcttggaaat ggcgcaattt
     9541 ttagctaaga gcctcttttg tacggataaa gttggcgtct taccatgtga gaaatgccga
     9601 agttgcaagc tgattgaaca ggaagagttt ccagatgtca ccttgattaa gccagtcaat
     9661 caggtcatca agacagaacg cattcgggaa ttggtgggac agttttctca agcagggatt
     9721 gaaagccagc aacaggtctt tattatcgag caagcggata aaatgcatcc caacgcagcc
     9781 aattctctgc tcaaggtcat cgaagaaccc cagagtgaag tttatatttt cttcttgact
     9841 agcgatgagg aaaagatgtt accgacaatc cgaagtcgga ctcagatctt ccactttaaa
     9901 aagcaagaag aaaaacttat cttactctta gaacaaatgg gacttgttaa gaaaaaagcg
     9961 actcttttag ctaagtttag tcaatcgcga gctgaagcag aaaagttggc taatcaggca
    10021 agtttttgga ccttggtcga tgaaagtgaa cgcctgctga cttggttagt agctaagaaa
    10081 aaagaaagtt atctacaggt tgccaaatta gccaacttgg cagatgataa ggaaaaacag
    10141 gatcaggttt tacggattct tgaagttctc tgtgggcagg acctcttgca ggtaagagta
    10201 agagtgattc tacaagattt actagaagct agaaaaatgt ggcaagctaa tgtcagcttt
    10261 caaaatgcca tggaatatct ggtcttgaaa gaaatataaa ctcaaaaatg aatgataaag
    10321 aaaggaaagg gctgttttat ggacaaaaaa gaattatttg acgcgctgga tgatttttcc
    10381 caacaattat tggtaacctt agccgatgtg gaagccatca agaaaaatct caagagcttg
    10441 gtagaagaaa atacagctct tcgcttggaa aatagtaagt tgcgagaacg cttgggtgag
    10501 gtggaagcag acgctcccgt caaggccaag catgttcgcg aaagtgtccg tcgtatttac
    10561 cgtgatggat ttcacgtatg taatgatttt tatggacaac gtcgagagca ggacgaagaa
    10621 tgtatgttct gtgatgagtt gttatacagg gagtaagcat gcaaattcaa aaaagtttta
    10681 aggggcagtc tccctatggc aagctgtatc tagtggcaac gccgattggc aatctagatg
    10741 atatgacttt tcgtgctatc cagaccttga aagaagtgga ctggattgct gctgaggata
    10801 cgcgcaatac agggcttttg ctcaagcatt ttgacatttc caccaagcag atcagttttc
    10861 atgagcacaa tgccaaggaa aaaattcctg atttgattgg tttcttgaaa gcagggcaaa
    10921 gtattgctca ggtctctgat gccggtttgc ctagcatttc agaccctggt catgatttgg
    10981 ttaaggcagc tattgaggaa gaaattgcag ttgttacagt tccaggtgcc tctgcaggaa
    11041 tttccgcctt gattgccagt ggtttagcgc cgcagccaca tatcttttac ggctttttac
    11101 cgagaaaatc aggccagcaa aagcaatttt tcgactcgaa aaaagactat cctgaaactc
    11161 agatttttta tgaatcgccc catcgtgtgg cagatacatt ggaaaatatg ctagaagtct
    11221 acggtgaccg ctccgttgtc ttggtcaggg aattgaccaa aatctatgaa gaataccaaa
    11281 gaggtacaat ttctgaattg ctggaaagca ttgctgaaac gtctctcaag ggtgaatgtc
    11341 ttctgattgt tgaaggtgcc agtcaggatg tggaagaaaa aggcgaggaa gacttgttct
    11401 tagaaatcca agctcgtatt cagcaaggta tgaagaaaaa tcaagctatt aaggaagtag
    11461 ctaagattta ccagtggaat aagagtcaac tctacgctgc ctaccacgac tgggaagaaa
    11521 aacaataaag ggagacagga tgtaataatt ctgtctgttt ctgtttaact taattagtga
    11581 tgataatata aagatgtatc acttggtata gaagctttgg tattaagttt tttattaagc
    11641 ccatacggaa taccgatggt tggagcagca gttatagcgt tcttagaagg tataaataga
    11701 aaaataaggt cattttaaat caaaggattg ataaatcaga aagaaggtga ttttttgcga
    11761 acatacgaaa ataaagaaga actaaaagct gagatagaga aaacatttga gaaatatatt
    11821 ttagaatttg ataatattcc agaaaattta aaagataaga gagctgatga agttgacaga
    11881 actccagcag aaaaccttgc ttatcaggtt ggttggacca acttggttct taaatgggaa
    11941 gaagatgaaa gaaaggggct tcaagtaaaa acaccatcgg ataaatttaa atggaatcaa
    12001 cttggtgaat tatatcagtg gttcacagat acctacgctc atttatctct gcaagagttg
    12061 aaagcaaaat taaatgaaaa tattcattct atctctgcaa tgattgattc gttgagtgag
    12121 gaagaattat ttgaaccgca tatgagaaag tgggctgatg aagcgactaa aacagcgact
    12181 tgggaagtgt ataagtttat tcatgtaaat acggttgcac cttttggaac tttcagaact
    12241 aaaatcagaa aa
//
LOCUS       DAVLSL010000052        11829 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLSL010000052 DAVLSL010000000
VERSION     DAVLSL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11829
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.053"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            118..771
                     /locus_tag="VXN30_001109"
     CDS             118..771
                     /locus_tag="VXN30_001109"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1357738.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            781..1245
                     /locus_tag="VXN30_001110"
     CDS             781..1245
                     /locus_tag="VXN30_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1357739.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1258..2526
                     /locus_tag="VXN30_001111"
     CDS             1258..2526
                     /locus_tag="VXN30_001111"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1357740.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     NATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2540..2800
                     /locus_tag="VXN30_001112"
     CDS             2540..2800
                     /locus_tag="VXN30_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357741.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2811..4172
                     /gene="pdrM"
                     /locus_tag="VXN30_001113"
     CDS             2811..4172
                     /gene="pdrM"
                     /locus_tag="VXN30_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1357742.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4326..5294
                     /locus_tag="VXN30_001114"
     CDS             4326..5294
                     /locus_tag="VXN30_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1357743.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5310..6302
                     /locus_tag="VXN30_001115"
     CDS             5310..6302
                     /locus_tag="VXN30_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1357744.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6520..7563
                     /locus_tag="VXN30_001116"
     CDS             6520..7563
                     /locus_tag="VXN30_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1357745.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7627..9312
                     /gene="lpdA"
                     /locus_tag="VXN30_001117"
     CDS             7627..9312
                     /gene="lpdA"
                     /locus_tag="VXN30_001117"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1357746.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9376..10365
                     /locus_tag="VXN30_001118"
     CDS             9376..10365
                     /locus_tag="VXN30_001118"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1357747.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10438..11508)
                     /gene="xerS"
                     /locus_tag="VXN30_001119"
     CDS             complement(10438..11508)
                     /gene="xerS"
                     /locus_tag="VXN30_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1357748.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAKNIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3495 a         2217 c         2648 g         3469 t
ORIGIN      
        1 tcacgtattc aaatcgtata tatagtcgat ttattaaatc tttagataaa ctgtaaaaag
       61 tggtttccat agccactttt tgctataata gaggcagtaa aaatgaagga gtcagctatg
      121 gaacactcgt cttggcatgc tttgattaag gcgcaattac ctgagggtta tttcgggaaa
      181 atcaatcagt ttatggagca ggtctattct caggggatta tttatccacc caaggaaaag
      241 gtttttcagg ctctcttgac aacactgctt gaagaagtta aggtggtaat tctagggcaa
      301 gacccctatc acggaccagg tcaagcgcag ggcttgagtt tttctgtacc tgactctatc
      361 ccagctccac catccttgca aaatatcttg aaagaattgt cagatgatat cggagttaag
      421 aaatctcatg atttgacagc ttgggctgag caaggagtct tgcttcttaa tgcttgtttg
      481 actgttcctg ctggacaggc caatggtcat gctggtcaaa tctgggagcc ttttactgat
      541 gctgtgattc aggtggtcaa tcatctagat agaccagtcg tttttgtact ctggggagct
      601 tatgcacgta agaagaaggc cttagttacc aatcctcatc acttgattat tgaatcagcc
      661 catccaagtc ctttgtcggt ttatagagga ttttggggtt ccaagccctt ttccaaggcc
      721 aatgcattct taaaagagac aggacaagag ccaatcgatt ggcttagata aggagaaaat
      781 atgcctcagt tagcgacgat ttgctacatt gataatggga aagaactgct catgctccat
      841 cgtaataaga aacccaatga tgtccatgaa gggaaatgga ttggtgtggg tggtaagcta
      901 gagagaggag agacgcccca ggaatgcgcg gcgcgtgaaa tccttgaaga aacagggctc
      961 aaagccaagc cagttctaaa aggtgtcatc acttttcctg aatttacacc agatttagac
     1021 tggtacacct atgtttttaa ggtgacggag tttgagggcg acttgattga ctgcaatgag
     1081 gggacgctag aatgggttcc ctatgacgag gttttgagca agccgacttg ggaaggtgac
     1141 cacacctttg ttgagtggct tttagaggat aaacccttct tttcagccaa gtttgtttat
     1201 gatggggata aattgttgga tacccaagtt gatttctatg aataaaggag aaagcagatg
     1261 ctactaatca aaaatggtcg tgtaatggat cctaagtctg gtttggatca agtttgtgat
     1321 gtcttagttc aagatgggaa aattatcaaa attgcgcctg agatcacgga agaaggagca
     1381 gaaacgatta atgctactgg tcttgtggtt gctcctggtt tggtcgatat tcatgtgcat
     1441 ttccgtgaac ctggtcaaac acataaagaa gacattcata ctggtgccct agcagccgct
     1501 gcaggtggtt ttactactgt cgtcatgatg gctaatacca gtccaaccat ttcagacgtg
     1561 gagactttgc aagcagttct ccagtcagct gccaaagaga agattaatgt caagacagtt
     1621 gcgaccatta ctaaaaactt taatggtaaa aacttgactg actttaaggc gctcttagaa
     1681 gctggtgcgg ttggtttctc tgatgacggt attccgcttg agagcagtaa gattgtcaag
     1741 gaagccatgg aggaagccaa aaaactcaat acctttatca gccttcatga ggaagatcca
     1801 ggtttgaacg gtgttcttgg ctttaatgaa aatattgcta gagaacattt tcatatctgc
     1861 ggtgctactg gggtggctga gtacgctatg atggcgcgtg atgtcatgat tgcctatgca
     1921 actaaagccc atgttcacat ccagcatttg tctaaggaag aaagtgttaa agtagtggag
     1981 tttgctcagg ggttaggtgc agaagtcaca gcagaagtag cgccacagca tttctctaag
     2041 acagaagcac ttcttttaac acaaggtagc aatgctaaga tgaatccacc gcttcgtttg
     2101 gaatcagacc gtcgtgccgt tatcgaaggt ctcaaatcag gtgtcatcac agttattgcg
     2161 actgaccacg cgcctcatca tgtagatgaa aaaaatgttg aggatattac caaagcgcca
     2221 tctggtatga ctggcttaga aacatccctg tctctcagct tgacctattt agtagaagct
     2281 ggtgagttga gcttgatgga attacttgaa aaaatgacat acaacccagc caagctttac
     2341 aactttgaag caggttactt ggctgagaat ggtccagcag atatcactat ttttgatgcc
     2401 aaggctgacc gccttgtgga ctcccatttt gcttccaaag cagctaattc accattcatc
     2461 ggtgaaacct taaaagggca ggttaaatat accatctgta agggacaaat cgtctatcaa
     2521 gcttgatagg agttagaata tgaattattt tcgaaaacgt agggagagac aagcgaaatc
     2581 taatagtgga atttactgtc ccgcagctaa tcgttccaaa attcaatatg aaactaaaga
     2641 aaaagctgac catgcggttc aatatgataa cggaggttta gttagaagct attattgtcg
     2701 tacatgtgcg tgttggcata ctacttcaaa atctaataaa cctccgctaa gtttgaaaaa
     2761 ctatggtctg aaactctttg gtttagataa atcagaatag aaagagaagt atgtataaga
     2821 caaagtgttt acgagagaag ttagtattat ttttaaaaat tttcttccca atactgatct
     2881 accaatttgc caattattct gcctcttttg ttgatactgc aatgacaggt caatacaaca
     2941 ctatggactt ggctggtgta tctatggcaa ccagtatctg gaatcctttc tttacatttc
     3001 taacagggat tgtgtcagcc ttggtgccta tcattggtca ccatcttggt cgaggaaaaa
     3061 aggaagaagt tgcgtctgat ttttaccaat ttatttattt ggccttgggc ctatctgtgg
     3121 tcttgctggg gatggtactt ttcttggcac caacaatctt gaatcatatt gggttagaag
     3181 cagcagtagc ggcagtagcg gttcgctatc tttggttttt atctatcggg attatcccct
     3241 tgttgctctt tagcgtcatt cgttccttgc tggattcgct gggcttgacc aaactgtcca
     3301 tgtacctcat gcttttgtta ctccctctca atagcggatt taactatctc ttgatttacg
     3361 gtgcctttgg tgttccagaa ctgggagggg ctggtgctgg tttaggaaca tccttggcct
     3421 actgggtctt gcttgggatt tctgttctgg ttttatttaa acaggagaag ctcaaagcct
     3481 tacaccttga gaaacgaatt ccacttaata tggataaaat taaggaagga gttcgtttag
     3541 gtctgcctat tgggggaact gtcttcgcgg aagtggctgt cttttcagtg gttggcttga
     3601 ttatggctaa gttttcgtcc ttgattatag ctagtcacca gtcagctatg aacttttcaa
     3661 gtcttatgta cgcctttcct atgagtatct catcggctat ggctattgtc gtttcctatg
     3721 aagtgggagc caagcgattt gatgatgcga aaacctatat tggtctagga agatggactg
     3781 ccctcatttt tgcggccttc accttaacct tcctttacat ttttagggga aatgtggcca
     3841 gtctttatgg taacgaccca aaatttatcg atttgacagc gcgtttttta acttatagtc
     3901 ttttcttcca gttagcagat acctttgcgg cgccgcttca gggaattttg cgggggtata
     3961 aggatacagt tattcctttt taccttggtt tgcttggtta ttggggcgta gcaatccctg
     4021 tggctacgct atttgattcc ctaacagatt ttggagctta ttcttactgg atcggcttga
     4081 ttattagttt gattgtgagc ggggcgctct accgttggcg tttaactgtg attatgaaga
     4141 gatttgaatc tttagcaaaa tccaaacgct aaaaagtttg tgaaaaaata ttcttgacaa
     4201 gaaaatccct tgttttcacg gggttttctt ttttattttg tgaaatttct tttagaaaaa
     4261 ctttgacagt atttgccaaa agcggtaaaa tagtaaggta agaagaaagt tagaaaggca
     4321 agaagatgtc aactttagat aaaaatcttt tgctagaaat gttccgtaag atggaagaaa
     4381 tccgtcgcat ggacttaaaa attgcacaat tagtaaagaa agggaaagtg ccaggaatga
     4441 cgcacttttc tgttggtgaa gaggcagcta acgtgggggc tatgttagct ctcaatccag
     4501 atgatctgat tacctcaaac caccgtggac acgggcaagc tattgctaaa gggattgacc
     4561 tcaacggaat gatggctgaa atccttggta aatacactgg aacctgtaaa gggaaaggtg
     4621 gatctatgca tatcgctgac cttgatgctg ggaaccttgg tgccaatggt atcgtaggtg
     4681 gtggtatggg gatcgctgtc ggtgcagccc tcagtcagca aatgcaaaat accggtaaaa
     4741 tcgttgtttg cttctttgga gatggtgcga ccaatgaagg tgttttccac gaagcagtga
     4801 acatggcttc tatctggaac ctgccagtca ttttctattg cattaacaac ggttacggta
     4861 tctctgcgga tatcaagaaa atgaccaata tagaacatat ccatcaacgt agcgccgctt
     4921 atggaattcc tggaatgttc atcgaagacg gtaacaatgt catcgatgtc tatgaaggat
     4981 ttcagaaagc tgtagaccat gttcgcagtg gcaatggttc agtcttgatt gaaagtgtaa
     5041 cttatcgctg gctcggtcac tcatcatctg accctggtaa atatcgtacg cgtgaagaag
     5101 tggaattgtg gaaacaaaaa gatccaatcg aaaacctccg caattacctt attgaaaata
     5161 acattgcaag tgccgaagaa ttggaagaaa tccaagcgca agtaaaggaa gcagtagaag
     5221 cttctgttaa atttgcagag gaaagcccat tcccaccgct tgaatcagcc tttgaagata
     5281 tttacgcaga ctaaggagaa agagataaaa tggaaacaaa aacaatgtcc ttccgtgaca
     5341 ccattatcct tgctatgtct gaggaaatgc gtcgcgatga aaatgtgttc ttgatgggag
     5401 aagatgtcgg tgtcttcgga ggagacttcg gtacttctgt tggaatgctt gaagaatttg
     5461 gtccagaacg tgtccgtgac tgtccgattt ctgaagctgc catctctgga gcagcagcag
     5521 gagcagccat gacaggactt cgtccaatcg tcgatatgac cttcatggac ttctctgtta
     5581 ttgccatgga caatatcgtc aaccaagctg ctaaaacacg ttacatgttt ggtggtaaag
     5641 gtcaggttcc aatgactgtt cgttgtgcag ctggtaacgg agttggctct gccgcccagc
     5701 actcacaatc tctagagtct tggtttactc acattccagg acttaaggtt gtggcacctg
     5761 gaacacctgc ggacatgaaa ggcctgctta agtcttctat ccgtgataac aacccagtta
     5821 ttatacttga gtacaagtca gaatttaacc aaaaagggga agtgccagtt gatccagact
     5881 acacaattcc acttggggtt ggtgaaatca aacgccaagg aacggatgta acagttgtta
     5941 cttatggaaa aatgcttcgc cgtgtggttc aagctgctga agaattagca gaagaagaaa
     6001 tttcagttga aattgttgac ccacgtaccc ttgttccgct tgataaggat atcatcataa
     6061 actcagtgaa gaagactggt aaggttattc tggtcaacga tgcccacaaa acaagtggct
     6121 atatcggtga aatttcagct attatttcag aatcagaagc atttgactat ctagatgcac
     6181 caatacgccg ttgttcagga gaagatgtgc cgatgcctta tgcacaaaac ctagaaaatg
     6241 caatgattcc aacagttgaa agtatcaaag atgcaatccg aaaaacttat aacaaagact
     6301 agagttacat aatataagtt atgtaactaa aataaagctg acgagaaact ttgtatcttt
     6361 taggtgcaga gctcttcgtc attaatatct tatattcgag cacgggctaa aagctcggaa
     6421 aaaagataaa tctccttggc ttcatcgaag ccatcgtcga tttcctattt ttcagtcgct
     6481 tttgacgccc tttgtatcat aaatagaatt ggagaggtca tggctgatga caagctaaga
     6541 gcgactcctg cagctagaaa gttagcggat gatttaggga taaacctcta cgacgtttct
     6601 ggctcaggtg caaacggtcg tgtccacaaa gaagacgtgg aaacttataa agacacaaac
     6661 gtggttcgca tttcgccact tgcaaaacga attgccctcg aacataacat tgcttggcag
     6721 gaaatccaag gaaccggtca tcgtggtaaa atcatgaaga aggatgtttt ggccctgctt
     6781 cctgaaaata tcgaaaacga tagcatcaaa tctcctgctc agattgaaaa agtggaagaa
     6841 gtccctgata acgtaacacc atatggtgaa atcgagcgta ttccaatgac accaatgcgt
     6901 aaggttattg cccaacgcat ggttgaatct tacttgactg cgccaacctt caccctcaac
     6961 tatgaagttg atatgactga aatgttggct cttcgtaaga aggttcttga gccaatcatg
     7021 gaagcaactg ggaagaagac tactgtaaca gaccttcttt cgcttgcagt tgttaagact
     7081 cttatgaaac acccatacat caacgcttca ttgacagaag atggcaagac tattatcact
     7141 cacaactatg tcaatcttgc catggcagtt gggatggata atggattgat gacacctgtt
     7201 gtttacaatg ctgagaagat gagtctttca gaactggtgg tagcctttaa ggatgttatt
     7261 ggccgtacct tggatggtaa attggctcca agtgagttac aaaattcaac attcacaatc
     7321 agtaatttgg gaatgtttgg tgttcagtcc tttggtccga ttattaacca acccaactca
     7381 gctatccttg gtgtcagttc gacaatcgag aagccagttg tcgtcaatgg tgaaattgtg
     7441 attcgcccaa tcatgagttt aggattaacc attgaccacc gtgtcgtaga tggtatggct
     7501 ggtgctaagt ttatgaagga cttgaaagag ttgattgaaa cgccaatctc aatgttaatt
     7561 taagagcaag agtattcatt tttaagatat agataaagga aggaagacat ggccttagaa
     7621 ataattatgc caaaagccgg cgtggatatg acagaaggac aaatcgtcca atggaataaa
     7681 aaagtcggag aatttgtaaa agaaggagaa atccttttgg aaatcatgac tgataaagtc
     7741 agcatggaat tggaagccga agaagatggg tacttgattg ccattctcaa aggagatggt
     7801 gaaactgtcc ctgtaacgga agttatcggt taccttggtg aagaaaggga aaacatccca
     7861 acagctggag cagcatcgcc agaagctagc cctgtacctg tagcaagtac ctcaaacgat
     7921 gatggtaaga gcgatgatgc ttttgatatc gttgtgattg gtggaggtcc tgctggttat
     7981 gttgcagcca ttaaagctgc ccaactcggc ggtaaggttg cccttgttga gaaatctgaa
     8041 cttggtggaa cctgcttaaa ccgtggatgt attccaacca agacctacct tcatagcgct
     8101 gaaattattg aaaatatcgg tcatgctgca aatcgtggta tcgtgattga aaatcctaac
     8161 ttcactgttg atatggaaaa acttttagaa actaaatcta aagttgttaa tactcttgtt
     8221 ggtggtgttg caggacttct tcgtagctac ggagttgctg ttcataaagg aattggtaca
     8281 atcactaaag acaagaacgt cttggtaaat ggttctgaat tgcttgaaac caagaaaatc
     8341 attcttgctg gtggttcaaa agtcagcaag atcaacgtcc ctggtatgga atctccactt
     8401 gtcatgacta gtgatgacat tcttgaaatg aacgaagtgc cagaaagcct tgttatcatc
     8461 ggtggtggag ttgtcggtat cgaactcggt caggccttca tgacatttgg ttcaaaagtg
     8521 actgttatcg aaatgatgga ccgtatagtt ccagctatgg atgtggaagt ttctaagaac
     8581 cttcgcttga tccttgagcg taaaggaatg accatcttga ctggtactaa actgcaagaa
     8641 atcattgagg aaaatggtca acttcgtatc aaggttgaag gaaaagacga tatcatcgca
     8701 agcaaagctc ttctttcaat tggtcgtatg ccagaccttg aaggtattgg agaggttgag
     8761 tttgaattgg atcgtggttg tatcaaggtc aacgaataca tggaaacttc agttccaggc
     8821 atttatgcac caggtgacat caacggtact aagatgttgg ctcacgcagc tttccgcatg
     8881 ggtgaagttt ccgctgaaaa tgcccttaaa ggaaatcatg cagttgccaa attgaatttg
     8941 actcctgcag ccatctacac tcttcctgaa gtagcagcag taggtttgac agaagaacaa
     9001 gcccgtgaga aatacgatgt tgccatcggt aagtttaact ttgctgctaa cggtcgtgct
     9061 attgcatctg acgcagctca aggtttcgta aaagttatcg ctgataagaa atacggagaa
     9121 atccttggtg tacacattat tggtcctgca gccgcagaat taataaacga agcatcaagc
     9181 atcatcgaaa tggaaatcac tgttgaggaa atgctgaaga ccatccacgg gcacccaact
     9241 tactctgaag tgatgtacga agcgtttgca gatgttctag gaatggccat ccattcacct
     9301 aagaaaaaat aaaacgaaga tagattagac tggaaggtta tttccagttt ctatcatata
     9361 aagtaaaggg atatcatgaa atacattatc aatcattcaa atgacactgc ttttaatatt
     9421 gccttggaag aatacgcctt taaacacctt ttggatgagg atcaaatctt cctactttgg
     9481 attaacaagc catctatcat tgttggtcgt caccagaaca ctattgaaga aatcaaccgt
     9541 gattatgttc gcgaaaatgg tattgaggta gttcgccgta ttagtggtgg tggagctgtt
     9601 taccacgatt taaataacct caactacacg atcatctcca aagaagatga aaataaggca
     9661 tttgacttca agagcttctc aactccggtt atcaatacct tggctcaact cggggttaaa
     9721 gctgagttca caggtcgtaa tgaccttgag attgatggta agaagttctg tggcaatgcc
     9781 caagcctata tcaacggccg tatcatgcac cacggttgct tgctctttga cgttgatttg
     9841 tcagtcctcg caaatgccct taaggtttca aaagataaat ttgaatcaaa aggtgtgaaa
     9901 tccgtccgtg cccgtgtaac caatattatc aatgaattac caaaaaaaat cacagtcgaa
     9961 aaattccgtg atttactctt ggaatacatg aaaaaagagt acccagagat gactgaatac
    10021 gtcttttcag aagaagaatt ggccgaaatc aatcgcatca aggatactaa gtttggaact
    10081 tgggactgga actatggtaa atcacctgaa tttaacgtcc gtcgtggaac caaattcact
    10141 agtggtaagg ttgaagtctt tgctaacgtt actgaatcaa aaatccaaga catcaagatt
    10201 tatggtgact tctttggtat tgaagacgtt gctgcagtag aagatgtcct tcgtggggtg
    10261 aaatacgaac gcgaagatgt tcttaaggcg ctaaaaacca ttgatatcac acgctacttc
    10321 gctggtatta gccgtgaaga aatcgctgaa gcggtagttg gataaatcaa aaagaagttg
    10381 gccaactaac caacttcttt ttttgatatt tatttacata atttaaaata cgtaaaatca
    10441 taaactatcc agagcattct tttgttcatc actaacaata tgggtataga ggtcagtgac
    10501 ttgtgtgcta gcatgtccta gttggtgact gactaaaact tgtgatttag tcgcatcata
    10561 gagcctagtt gctagtgtat ggcgcagttt atggggtgtt acacgcactt taaaatcctc
    10621 tgagtattta gcaaccattt tctcaacgct agaagcatcg atacgattag gaacacctct
    10681 gtagagagtt aaaaaaaggg ctgtatctgt tttttccgtt ttatagcgtt gattccgaat
    10741 ggccagataa ttctctaaat aaggtttagc aaaagcagcg acattgactg agtcacgttt
    10801 gccacctttt cgagtaacat caataaccat catttttaga ttgagatctc ttagatctag
    10861 attaacagct tcagataagc gaacaccaga tgccaagaga agggcaataa tggctaaatc
    10921 tcgttcttta tttttgttga atgatgagag agctcgattt gaaagctgtt gtgggtgctc
    10981 ttgatcgata taagttagaa aaccttctgt ttcatcacct agaaagagtt tttgcttgat
    11041 atttttagct ctggcagcaa gggtttcttt ctttttcttg gttgaaactt ttttcattac
    11101 attacgatag aaataaggtt ccccctgatc gttttcaacc tcttcggtta gatacttgta
    11161 aagactagaa agtgctgata aggttcgatt gatagttgtc tgtgaaacac cttgttttgt
    11221 tgtattagca ttcagcaagg gacgttcacg tagataaagg ataaaggatt ccatgtcttt
    11281 cttagacata ttttccaaaa ctgataaagg aatatcggat attttatcag cgtttgaaat
    11341 accagactcc aaaacccagc tgaaaaatcg gtcatattcc ttaaggtatt cgtacagggt
    11401 tgtaaaactg tagggcacag ccagcttaga ttggtagtat tccagaacat accagggcat
    11461 gagttgtttt agtttgtcga ttcgttccag taaaatctca cgtttcatgt attttctcca
    11521 taagtaagta tactagtagt atagcataga cttgtatatt tttcaagaaa attatagttt
    11581 ttcagaaaga tgttatagga gtttttcaaa gtgataaaaa gacttagacc tacaaacaaa
    11641 acagaataag gaaaataata aaaatctcac ccactaaact tctgttaata ctacatagct
    11701 agaaattgat ttgattgtcc tgattgtttt atcctatttc tatttcattt cactatataa
    11761 ttgctattgt tttctttttc atgattttaa tcttactgta cagatttgat acagtaaact
    11821 cctttctag
//
LOCUS       DAVLSL010000053        11768 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLSL010000053 DAVLSL010000000
VERSION     DAVLSL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11768
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.054"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            85..672
                     /locus_tag="VXN30_001120"
     CDS             85..672
                     /locus_tag="VXN30_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1357749.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEIGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            599..2242
                     /locus_tag="VXN30_001121"
     CDS             599..2242
                     /locus_tag="VXN30_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1357750.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLVQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2253..3614
                     /locus_tag="VXN30_001122"
     CDS             2253..3614
                     /locus_tag="VXN30_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1357751.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            3671..3799
                     /gene="blpC"
                     /locus_tag="VXN30_001123"
     CDS             3671..3799
                     /gene="blpC"
                     /locus_tag="VXN30_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1357752.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(3870..5210)
                     /locus_tag="VXN30_001124"
     CDS             complement(3870..5210)
                     /locus_tag="VXN30_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1357753.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5224..5961)
                     /locus_tag="VXN30_001125"
     CDS             complement(5224..5961)
                     /locus_tag="VXN30_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1357754.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(5966..6304)
                     /locus_tag="VXN30_001126"
     CDS             complement(5966..6304)
                     /locus_tag="VXN30_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1357755.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6580..6909
                     /locus_tag="VXN30_001127"
     CDS             6580..6909
                     /locus_tag="VXN30_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1357756.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7077..8126)
                     /locus_tag="VXN30_001128"
     CDS             complement(7077..8126)
                     /locus_tag="VXN30_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1357757.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8123..8854)
                     /locus_tag="VXN30_001129"
     CDS             complement(8123..8854)
                     /locus_tag="VXN30_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357758.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            8922..9332
                     /locus_tag="VXN30_001130"
     CDS             8922..9332
                     /locus_tag="VXN30_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1357759.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9342..9629
                     /locus_tag="VXN30_001131"
     CDS             9342..9629
                     /locus_tag="VXN30_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357760.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            9791..9985
                     /locus_tag="VXN30_001132"
     CDS             9791..9985
                     /locus_tag="VXN30_001132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357761.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(10319..11455)
                     /gene="dnaJ"
                     /locus_tag="VXN30_001133"
     CDS             complement(10319..11455)
                     /gene="dnaJ"
                     /locus_tag="VXN30_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1357762.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         3810 a         2554 c         2108 g         3296 t
ORIGIN      
        1 tttaatgact attcaagatt tcataaaata tgaacttagt tttatgacat aatagaccta
       61 tccactatat gaaaggaatt gccaatgact tcttataaac gtacatttgt tcctcaaata
      121 gatgcgagag actgtggtgt cgctgcctta gcctcgattg ctaaattcta tggttcagat
      181 ttttctctag ctcacttgag agaacttgca aagaccaata aagaagggac gactgctctt
      241 ggcattgtaa aagccgctga tgaaataggc tttgaaacaa gacctgttca agcagataaa
      301 acgctctttg acatgagtga tgtcccctat ccatttatcg ttcacgttaa caaagaagga
      361 aaactccaac attactatgt tgtctatcaa acaaagaaag actatctgat tattggtgat
      421 cctgaccctt ctgtaaaaat cactaaaatg tcaaaagaac gctttttcta tgaatggact
      481 ggagtagcta tttttctagc taccaaaccc agctatcaac cccataaaga taaaaagaat
      541 ggtctactaa gcaagcttcc ttcctctgat tttcaaacaa aaatctctca ttgcttacat
      601 tgttctctca agcttattgg tcactattat caatataggt ggttcttact atctccaagg
      661 aatcttggat gaatacattc caaatcagat gaaatcaact ttaggaatca tctcagttgg
      721 tctggttatc acctatatcc tccaacaagt catgagcttc tccagagatt atctcctaac
      781 cgttctgagt cagagattaa gtattgatgt gattttatcc tatattcgcc atatttttga
      841 acttcccatg tctttctttg cgacacgtcg tacaggagaa atcatttcac gattcacaga
      901 tgctaactct attatagatg ccttggcttc taccattctt tctctttttc tggatgtttc
      961 tattctgatt cttgtaggag gcgtcttact ggtacaaaac cctaatctct tccttctttc
     1021 tcttatttcc attcctatat acatgttcat catcttttct tttatgaaac ctttcgaaaa
     1081 aatgaaccat gatgtcatgc aaagtaattc tatggttagc tctgccatta tcgaagatat
     1141 caacgggatt gaaactataa agtcgctcac gagtgaagaa aatcgctatc aaaatataga
     1201 cagcgaattt gtagattatt tggaaaaatc ctttaagctc agtaaatatt ctattttaca
     1261 aacgagttta aagcagggaa caaaattagt tctgaatatc cttatcctat ggtttggcgc
     1321 tcaattagtc atgtcgagta aaatttctat cggtcagctg attaccttta acacactttt
     1381 ttcttacttt acaactccta tggaaaatat tatcaacctc caaaccaaac tccaatctgc
     1441 gaaggtcgct aataaccgtt tgaacgaagt ctatctagtc gaatctgaat ttcaagttca
     1501 agaaaaccct gtccattcac attttttgat gggcgatatt gaatttgatg acctttctta
     1561 taagtatggt tttggacgag ataccttaac agatattaat ctcacgatta aacaaggaga
     1621 taaggttagc ctagttggag ttagtggttc tggtaaaaca actttagcca aaatgattgt
     1681 caatttcttt gaaccctaca aaggacatat ttctatcaat catcaggata ttaaaaacat
     1741 tgataaaaaa gtcttgcgcc gtcatattaa ttacctacca caacaagcct atatctttaa
     1801 tggctctatc ttggaaaact taactttggg cggtaatcat atgattagcc aagaagatat
     1861 tctaagagct tgtgaattag ctgaaattcg tcaagacatt gaaagaatgc ctatgggcta
     1921 tcaaactcag ctctctgatg gagctggtct atcaggagga caaaagcaac gaatagccct
     1981 cgctcgtgct cttttaacta aagctcctgt tttaatacta gacgaagcta ctagcggtct
     2041 tgatgtcttg actgagaaaa aggttataga taatcttatg tctctaactg ataaaaccat
     2101 tctctttgta gcccatcgtc tcagtatagc cgaacgaacc aaccgtgtca ttgttcttga
     2161 ccaggggaaa atcattgaag ttggtagtca ccaagagtta atgcaggcgc aaggtttcta
     2221 ccatcatcta ttcaataaat aaggagaatg tcatgaatcc taatcttttt agaagcgtcg
     2281 agttttatca gagacgttac cataactatg cgacagtgtt cattatacct ctttcattac
     2341 tatttacttt catcttgatt ttctcccttg ttgccacaaa agaaattact gttacttccc
     2401 aaggagaaat cgcccctaca agtgtcattg cctccattca gtcaaccagt gataatccta
     2461 tcctagctaa tcatttagtg gcaaatcaag tagttgaaaa aggggactta ctcatcaaat
     2521 actctgaaac aatggaagaa agtcagaaaa ctgccttagc aactcaatta caaagacttg
     2581 agaagcaaaa agaaggactt ggaattttga aacaaagctt agaaaaagcg actgatcttt
     2641 tttctggcga ggatgaattt ggctaccata atacctttat gaattttact aaacaatccc
     2701 atgatattga actgggtatc acaaagacta acaccgaagt ttcaaatcaa gctaatcttt
     2761 ccaatagcag ttcatcagct attgaacaag aaattacaaa agttcaacaa caaattggag
     2821 aatatcaaga gttgagagat gctatcataa ataacagagc acgcttacca actggcaatc
     2881 cgcaccagtc aattttgaat cgttatcttg tagcctcaca aggacaaaca caaggaactg
     2941 cagaggagcc atttttatct caaattaatc aaagtattgc aggtcttgaa tcatctatcg
     3001 caagcctcaa aattcagcaa gctggtatcg gaagtgtagc aacttatgat aacagtttag
     3061 caaccaaaat tgaagtactc cgcactcagt ttttacagac agcctcacag caacaactaa
     3121 ctgtggagaa tcaattaaca gaattaaaag tacaactaga tcaagccaca cagcgtttgg
     3181 aaaacaatac cttaacctcc ccaagtaaag gtatcgttca tctgaacagc gaatttgaag
     3241 gtaaaaatag aattccaact ggtacagaaa ttgctcaaat attccctgtc atcacagata
     3301 caagagaagt actaatcact tactacgtat cttctgacta tctacctcta ctagataaag
     3361 gacaaactgt aagattaaaa ctggagaaga ttggaaatca cggcaccacc atcatcggcc
     3421 aacttcagac aattgatcaa actcctacca gaacagagca aggaaatctc tttaaattaa
     3481 ccgctcttgc aaaactatct aacgaggata gtaaactcat ccaatatggc ttacaaggtc
     3541 gcgtcactag tgtaactaca aagaaaacat attttgatta tttcaaagat aaaattttaa
     3601 cccattctga ttaattttca gataacactc tataactatt tattatctta tcaaaaagga
     3661 gaatcataac atggataaga aacaaaacct aacttcattt caagaactaa caactaccga
     3721 actcaaccaa attacaggtg gaggattgtg ggaagattta ttatataaca ttaatagata
     3781 tgctcattac atcacataag aacttcatca tccaatacaa ctataaaaaa ataagaccga
     3841 gaaacaagta ctctcggtct tatttttcat cattctgtat gtatcacagt aagtacctga
     3901 cgaaagactt gattttgaca ggtggtattt agactggtat taggatggct ttccacaatc
     3961 ttcatgacgg tatagagacc aactcctctc tcctcccctt tagaactggc tccaaaggag
     4021 aagatttcag aaatatcgat gccctcttct ttgatggagt tttcgatgat aaaggtctcc
     4081 tgtgctccat tttttaaaaa ggcgattgaa acatgaggtt gactagcttc cacactggct
     4141 tcaatagcat tgtcacaaag gatagacaca atggttagaa aatcaagtag actcatcccc
     4201 tcgacctgaa tctcctcagg aacttcgaca ttaaagacaa tgttcttatc tctggctttt
     4261 aaaaatttcc ctgctagaag acttttgagg gctttatcac gaatatttac caatctgccc
     4321 aggtcatatt tattgttctg caatttctga ctggaatcct ttaagacgga gccatagacc
     4381 tcttttatct gctccatatc ctcctcttca atgcccagac gtaagctagt caagaggttg
     4441 gtataatcat gacgaaagct ccgtacttcc ttgtaaagct cctctatatg ccgactatag
     4501 cgttccatat ctctatagcg cagggcctgc tcttgttcca atctctcata gagtttttcc
     4561 ttcaaatagg tatccaattt cttgataacc cccataaaaa agagtaggta aaagactagg
     4621 atgagatggc gaacagtctt tgattgaata ctttgttcat attcaaaaaa agacagactt
     4681 tccatgacta gatagtagcc acccattatc cagttaatct gagtcaggga cttttgaaag
     4741 gctttatcga gaatctcctt tctcaagcta gtaaaatcgt agtccaacca tttcaaaaaa
     4801 gctagagaaa tgaagaaatt gaaaattatt atacataacc cagtaaatga gtagccatca
     4861 tatacttgcc cttgtcccaa aaatggaagc acaaaatagg agactcctct ataaaagaga
     4921 ttcaccaata tcattggaaa gagaccataa aagaaaagga gttttttagg aagccctctc
     4981 aataataaga aagataagcc tatgccgtac aagggttcca taaaataaga taggtaaaca
     5041 tttcctacta tatagctaat catcacaaaa acaaaggcca acagtatctt aaaaagaaag
     5101 gccttaaaaa tcctctcgaa agtaagatca attccatcca ccttaaagaa gatgacaatt
     5161 tctagtccat tagtaacaag tgtatacaac aatatccaag caatgttcat aaattctcct
     5221 agctcagtgt aatttattga tggcctcaga cacttccctg accttataac gggcgattag
     5281 acaacttcca ccattgggaa agaaaagcag tttttctttc ttatccaaat gcaccacatt
     5341 tgcaggattg atgagaaaag agcggtggca ctgcaagaga cggggctcct gcttgaaaac
     5401 ctcctctaaa ctcgctgtaa attccagcct gtctgtcttg gtatagagaa taacacgatg
     5461 ggctctgggc gacgtttcga gatagtaaac ctctttaaaa ggatactgaa attgggcaaa
     5521 ttttgattta aagtaaaagc aatcttccgc cagactttta ctatcttgac tattggcata
     5581 gaggagggct gtctcgatcc gagattcaaa ctcctctgct gacaaggcct tatcaatgta
     5641 gtccaaagca gacacttggt agcgaaaaga caggggcata aactccgagt gagtcgtcac
     5701 aaagacaatc agggcataag gatcccgatc ccgaatcttt ctagccactt ccagtccctt
     5761 catctcttca tttcgaatct caatatccaa aaagaatagc tgatgggccc ccttctcatg
     5821 cacttcagcc agcagttggt ccggcttgcc aaatacctca aaagagctag gaatgatatg
     5881 atgtgctttc aaaagtttct caatcgtcgt ttcaattcta gtctgttggg aaaaatcatc
     5941 ctctaaaaca aatattctca tcttcttact ctccctcttt caaccatttt tgacgaattt
     6001 ctctatagcg acgtctggca tacttgacag cgtattgacc ttcttctccg agaaaaagga
     6061 ttctttcttg aaaatcaatc gattttaatt tatcaagatt aaccaaacaa tttcgatgac
     6121 atctcatcaa ggtttcccca cattggttct caagattact taaattttga agcatattaa
     6181 aactagcttc ttctgtaaca atctgtacgg tatgggcttt agttggatgt gtttggatat
     6241 agtagatatc gtcgatgttt actttataga ctgttttctg cgtctgaata atcatatatt
     6301 tcattggatt ctcctcctaa gaaaagaata acagaaaagg tctagcccaa gcacacccaa
     6361 tcctaaacag tctttgaaaa ctcctaaaag gattattttc tacaacatca tgatgagctt
     6421 acaactattt tatcataaaa ttcactcagc gcaattcaag acatttcaat gacaattaaa
     6481 gatttgaata aaaaaaaaga attaaaagtg ataaactaac agtatcaaaa cacagttgcc
     6541 agaagtaaga ctggcaccca gattaaagga ggcattccca tgacagatac agaccccatc
     6601 aaaagagctc atactttgat tactgactta aacaaagcct accaagcatg caaacaggca
     6661 agcgctgacg acgtccgctt tcaggagcaa ttaaactcta ttcttggttt tctagccaag
     6721 gctgaaacag tggataatcg attcttgatt gaactagaaa aattttacca gacttccagt
     6781 cttctcatgg gactcagcgc tcttgatcca gatgctccaa ctcgcgccgc ttggcgggcc
     6841 tatgaccgct tccactttga ccaagtcaag accaagttaa tactcaatga aaatcaaaga
     6901 gcaaactagg aagctagccg caagctgtac ttgagtacgg taagacgacg ctgacgtggt
     6961 ttgaagagat tttcgaagag tataagtctc tacggaccaa ccattatcct gtagtaacaa
     7021 aaaagaagtt gagacagact gaactcaact tcttttttag tgtcgtatca gcaatgttag
     7081 tcctgcatct gacgttttac ctgataaggc aaatacaaga ctagtaaaac caaaccagct
     7141 cctagtaagg ctagaagggc tagtttttct tggaaggtaa tcaacccaac aactaattcc
     7201 acaagtaaaa caaaactggt caatcctcga actacctcct gtaagccttt ttccttttgc
     7261 cccttgtcca gcggaaagag ttgggtcaaa tactggtagt caaaggcatg atagagggcc
     7321 agcaactgga agagcaagag gtagttaaag agaactacca ctgctgtcgc aatccaagct
     7381 tgctcgataa aaacctgcgc cagtacggaa agtaagagaa gacgaagact gagagcaaag
     7441 aggtcgccat ttcgcagata agaacgcaga tagagatttt gccaaatctt cccaggcacc
     7501 ttctgaacag cctttaagat aaagtccaga taggcacgac gcttaacgct gtttgaaatt
     7561 cccttgacct gcgtaaagag ggcaaagaaa cgaagcaaga cttgcttacg cttgctttct
     7621 tgggaaatga catagtccca atccagtcca gtttcagtga aaaatttgct ggccttttga
     7681 cgaaagtgga aatattttcc tacccccaat aaaagcacat agagcagaaa aactggcaag
     7741 ccataaccca ttgctaaaaa taaaggcgca aataacagca agaaaagggt ctgtacaaag
     7801 agccaaaaga ctagggaaat gccagtttga cgcttgagat ggagcttgat ttcctcttct
     7861 ccaactaaga gaaagagctt gtctggagcc tccatatagg tggcaattcc tccccaaagt
     7921 aaaagtaaaa cagacgtaat tcctacaaac aaaaggatag gccaatgatt ttcaggaaaa
     7981 tgttgtaaga gttgactgta ctggtaggct agaaagccca acaggacaag caggaacaag
     8041 acaaagtggt cattgagcac atagcgcaga taaccaagac actccttacg aaaggcctgc
     8101 tttctcttta aaaacaagtc tttcataggt cctcctcttt tgtcagagcc aagtaaatat
     8161 cattcaaact agcctcaggc atatcaaagg cttcgcgtag ttgcaggaga ttgcctttgg
     8221 cacgcacctc tcccttgtga agaatgacaa aggcatcaca catcttctcc gccgaatcca
     8281 gcacgtgggt actcatgaga atagacttgc ccttttgctt ctccacttcc aaaagctgaa
     8341 tcaaatcaga aatagccagc ggatcaagac caaggaaagg ctcatccacg atgaaaagac
     8401 ttggatccgc cacaaaagca cagataatca tgaccttctg cttcatccct tttgaaaaat
     8461 gaacagggaa ccagtctaat ttctgttcca aacggaacat ttttaacaag ggctctactc
     8521 gttcgaaagc cactttttgc tcaataccgt aagccatagc aaccgtttcg atatgctctc
     8581 tgagggtcaa ttcctcatac agactaggcg tctcaggaat gtagccaatc tgcttgcggt
     8641 agctagtcgc atctccttgc agagtcaggc cattgatatt gatggagcca ctataaggtg
     8701 ccaacagacc gataatctca ttgatcgtcg ttgatttccc agcaccattg agaccaatca
     8761 aaccgaccaa ctgcccactt tcaacagtaa aggacacatc tttcaaaaca ggaacatgaa
     8821 catagccacc tgtcaggttt ttaatttcta acatattttc tccaaatctg gtataatgta
     8881 gctatattat atcaaaattc agtacagtag aggtagattt tatgtcagat tgcatttttt
     8941 gtaaaatcat cgcaggggaa attcctgctt caaaagtata tgaagatgag caggtccttg
     9001 cctttcttga tatctctcaa gtaacactag gacacacctt ggtcgtgcca aaagaacact
     9061 atcgcaatct tttggagatg gatgctacga gcgccagcca actctttgcc caagtaccaa
     9121 aagtagctca aaaagtcatg aaagtcacta aggctgctgg tatgaatatc atttccaact
     9181 gtgaagaagt cgctggtcaa acagtttttc atactcacgt tcaccttgta cctcgctaca
     9241 gtgctgacga tgacctcaag attgatttta tcgcccacga accagacttt gacaaacttg
     9301 ctcaagtcgc tgaaaccatc aaaaacgctt aaggagttgc tatgaaatta tccaacctac
     9361 tgctatttgc aggagctgca gccggaagtt atctggtcac aaaaaatcgc caaaccatca
     9421 cagatgaagt cttgaatacc actgaccgcg ttcaagctat caaggacgat gtggatatta
     9481 tccaaaacag cctgcaaatc attgaccagc aaaaagaact tatcaaggaa taccaagaag
     9541 acttgactta caagtttaag gtcttggaaa aggatatcca gactagacta gctgtgataa
     9601 aagaaatgca gggaactgaa gataagtaaa aagagcccga cggctctttt ttaaaacgta
     9661 aacaagttat agtgaattga atttagaata gcacatcata gtttctaaag cattgttaga
     9721 gattacttta aaatcctctt atcaatttgt tcatattcta tttcaatcta ctatatatat
     9781 cccatcaact atgcatcata atttaggtaa ctcatactca ataaaaatca aaaagcaaac
     9841 tagaaagcta ggcacagact gctcaaaaca ccgttttaag gttgtggata gaactgacga
     9901 agtcagtaac catacccatg acaagccgac gctgacgtgg tttgaagaaa tttttgaaga
     9961 gtatcattca ccatttcaca actaaatcga agactttctg ccgtaatgaa aacacctgaa
    10021 acagcttggt ttcggctatc cggaaacttt aagacttagg ttcaaagttt aggtatggaa
    10081 cttcgaagga ggtcgctacc gtccgtcatt acttaggaaa gtcttaaaaa atctataaag
    10141 taaaaagagc ccgacagctc tttttgttta tactcaatga aaatcaaaaa gcaaactagg
    10201 aaactagcca cgggctgctc aaaacactgt tttgaggttg tagatacgac tgacgaagtc
    10261 agtcacatat atacggcaag gcaacgctga cgtggtttga agagattttc gaagagtatt
    10321 attctccatc aaaggcatct ttaatatggt caaagaagcc ttttttcttt ggatttactt
    10381 tcaagtcacc agcagccgcg aattctttca aggctacttt ttggcggtcg ttcaagcctg
    10441 tcggtgttac gacattaaca gtaacgtatt ggtcaccaac tgcaccgcca cgaaggctcg
    10501 gtgccccctt actacgtagg cggaacttct taccagtctg agttccctct ggaataacca
    10561 attcaacatc accgtgaaca gttggaatat ctactgtatc accaagagcc gcttggacaa
    10621 agttgaggtt gagattgtag aagatagtcg ttccttcacg ttcaaacttg tcgctagctt
    10681 ccacagaaac tactacatac aagtcaccat aaggtccacc gttaaagcct gcttcacctt
    10741 gaccagcgag gcgaatttgt tgacctgttt ccacaccagc agggattttc acatgtacgc
    10801 tatgagcttg tttctcatga cctgttccat gacaggttgt acatggatat ttgatttctt
    10861 ttcctcgacc gtgacagaca tcacaggtta cttggcgacg catcatacca agaggagtct
    10921 gcgtatcgac gttaatgaca ccagcgccat gacagcgtcc acaagtgact ggacttgtcc
    10981 ctggcttagc accagatcca ttacatgtac gacagccagc ttcacgatga tacttaactt
    11041 ccttctcagt tccgaagata gcttcttcaa aggtcaaatt gacacgatac tggagatcat
    11101 ctccttggcg aggagcgttt ggattgcgcg aagaaccgcc tccgccgaag aaacttgaga
    11161 aaatatcctc aaaaccaccg aagccacctg ccccattgaa accgccgaaa ccaccagctc
    11221 caccaaaacc accattggcg cctgcagcac catactggtc ataggcagca cgtttttggt
    11281 cgtcactcaa agtctcatag gcttcttgaa cttccttgta cttgtcctca gcaccaggct
    11341 ccttgttgat atctgggtga tattttttgg aaagcttacg ataagccttt ttgatttcgt
    11401 ctgccgaagc gtttttggat acccccagac gatcataaaa ttcagtattg ttcataattc
    11461 aagatacaaa gggcgtcaaa cggacacagc catctaggag ttttgacgac ggacgcttac
    11521 gtcctagaaa aaactatctt tttggcacag tccatagccc gtgttcagtt acgaacaccc
    11581 ttattccttt ctattgatgt tctgaatcga accaccattt aggtcgggtt caattccttt
    11641 cttttatatt tagtaagaaa ttctgaaacc cgtgttcagt tacgaacact ctttgttcct
    11701 ttctattgat gagacaaaac ccaaacaaat gagattccgt ccctttttat cgaaaaacag
    11761 aggctggg
//
LOCUS       DAVLSL010000054        11560 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLSL010000054 DAVLSL010000000
VERSION     DAVLSL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.055"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(113..1936)
                     /gene="dnaK"
                     /locus_tag="VXN30_001134"
     CDS             complement(113..1936)
                     /gene="dnaK"
                     /locus_tag="VXN30_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1357763.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2416..2940)
                     /gene="grpE"
                     /locus_tag="VXN30_001135"
     CDS             complement(2416..2940)
                     /gene="grpE"
                     /locus_tag="VXN30_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1357764.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(2967..4001)
                     /gene="hrcA"
                     /locus_tag="VXN30_001136"
     CDS             complement(2967..4001)
                     /gene="hrcA"
                     /locus_tag="VXN30_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1357765.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4165..4674)
                     /locus_tag="VXN30_001137"
     CDS             complement(4165..4674)
                     /locus_tag="VXN30_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1357766.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            5282..7615
                     /locus_tag="VXN30_001138"
     CDS             5282..7615
                     /locus_tag="VXN30_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1357767.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMNTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTIAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            7628..9091
                     /locus_tag="VXN30_001139"
     CDS             7628..9091
                     /locus_tag="VXN30_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1357768.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            9091..10659
                     /locus_tag="VXN30_001140"
     CDS             9091..10659
                     /locus_tag="VXN30_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1357769.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNS
                     YYEEVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLAR
                     FIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWAVADSHVTVIRV
                     LSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIV
                     DKIEQFFAHIDALI"
     gene            10716..11513
                     /gene="psrA"
                     /locus_tag="VXN30_001141"
     CDS             10716..11513
                     /gene="psrA"
                     /locus_tag="VXN30_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1357770.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT         3679 a         2066 c         2241 g         3574 t
ORIGIN      
        1 tggaatatta tctataaaac cttttgctgc ttcatagttg tcagcttgat tacaaaaatc
       61 attataaact tttcgtgtat tttttagata ctcatcatcc aatacactca tcttattttt
      121 ccgtaaactc tccgtctacg acgtcatcgc ctgcgtttcc tgttgcttgt gcgccttctg
      181 ctccttcttg agcttgttgc gctgctgcgg cttgttcgta gagtttcaca gcaaggcctt
      241 gagctttttc gttcaacgct tcaagtttcg ctttcatgtc gtccaagttg ttgtcttctt
      301 gagctttctt aaggtcatca agggcagctt gggcagcgtc acgttctgcg tcgaagcctt
      361 taccttcagt ttccttgatt gtcttttcag tcgcaaagat tgcttggtct acttcattac
      421 gaaggtctac ttcttcttta cgtttcttat cggcttcagc gtttgcttct gcatctttca
      481 tcatgcggtc gatttcttcg tcagtcaaac ctgagttcga ttggatgaca atagtttgtt
      541 ctttttgagt tccaaggtct ttggccttaa cagacacgat accgttcttg tcgatgtcaa
      601 atgttacttc gatttgagga attccacgag gtgcagctgg gatatcagtc aattggaagc
      661 gtccaagagt cttgttatct gctgccattg ggcgttcacc ttgaagaacg tggatatcaa
      721 cggctggttg gttgtctgct gctgttgaga agacttgtga tttagatgtt gggattgtag
      781 tgttgcgatc gataagtttt gtaaatactc cacccattgt ttcgatacca agtgacaatg
      841 gcgttacatc aagaaggaca acgtccttga catcaccagt aatcacacca ccttggatag
      901 ccgcacccat agcaactact tcatcagggt ttactgattt gtttggttct ttaccagttt
      961 cagctttaac agcttcaaca acggcaggga tacgagttga accaccaaca aggataactt
     1021 cgtcgatttc tgacaagctc aaacctgcat ctgaaagggc ttgacgaact ggaacttttg
     1081 tacgttcaac aaggtcacga gtcaaatcgt caaatttcgc acgagtcaaa gtcatttcca
     1141 agtgaagagg tccagcctca cctgcagtga taaatggcaa gctgatttgt gttgaagtta
     1201 caccagaaag gtctttcttc gctttttcag ccgcatcttt caaacgttgc attgccatct
     1261 tgtcagtaga caagtcgata ccgttttctt tcttgaattc tgctaccaag tggtcaatga
     1321 ttttttggtc aaagtcgtca ccaccaagtt tgttgtcccc tgcagttgac aatacgtcga
     1381 agacaccgtc acccaattca aggatagaga cgtcgaatgt accaccacca aggtcaaata
     1441 ccaagatttt ttcttcttta tcagtcttgt ccaaaccata agcaagagct gctgcagttg
     1501 gttcgttaac aatacgttct acttcaagac cagcaatttt accagcgtct tttgttgctt
     1561 gacgttgagc gtcgttgaag taagccggaa ctgtgataac agctttggtt actttctcac
     1621 caaggtagtc ttcagcgtag cctttcaagt attgaaggat catagctgag atttcttgtg
     1681 gagtgtattc ttttccattt gcagaaactt tttcagaagt tcccatctta gatttgatag
     1741 agataactgt atctgggttt gtgactgctt gacgttttgc agcatcacca acgatgattt
     1801 ctccgttttt gaatgagact acagatggag ttgtgcggtt tccttctggg tttgcgatga
     1861 ttttgctttc agttccttca agaactgcaa ctgctgagtt tgttgtacct aagtcaatac
     1921 cgataatttt agacatgtgt ttttctcctt gttagttaat tttctatttt tctttttgat
     1981 actcttctta agtggcgacg ccgtcagagc ttgacttcgt caatctcttt gactaaactt
     2041 tgagcctaag gtctcaaaat ttgcgcaaat agcgccacgg cgaagagtat cgtgtttggt
     2101 tcgcttcgct cactattaca aagccaaaca tattttatct ttcttcatag tttattgtcg
     2161 tttcggacaa gttttctatt atgtaaattg cgacacgaag agtcgaaatc gattttattt
     2221 cgacgacgag ttagtaagga agctaggcaa acgccatagc gattgccgtt ttctgacgag
     2281 ttgctttagc taccgtcaga attgccgaat cgaacacgcc ctaagctctg agtaaaaaag
     2341 atagattttc ttgtgttcat cgaacacttc gttaatctcc tatttttact gcgagctttt
     2401 tacgggcttt gtatcttagt tatacaccac taccattgct gggcgtagga tgcggtcatg
     2461 gagtttgtag cctttttgaa agacttgagc gatggtatct actgggtgtt catcgtctgc
     2521 tgggagagtt tggatggcca tatggtagtt atggtcaaat tcgccatctg ctgcgatttc
     2581 ttcaattcct tcttctttca aagcgtgaat caagctttct tgcaccatcc ccaagccctt
     2641 cttcacatca tctgtcaaac cttcaactgc aagtgcacgc tcaaggttgt caagagatgg
     2701 taagattgct tttgccaagt cctggctacg ataacgttgc aagttttgac gttcttcatt
     2761 ggcacggcgt tggatatttt gcatttctgc atgagcgcga agatatttgt tttcgaactc
     2821 atctgcacgt tcatttgcca agtccaactc agacttttca ggagttgttt cttcagctgt
     2881 ttccacaact tcctcttctt gaacttcttc tacttcttca ttttttatat cttgggccat
     2941 gttcgcctcc tttaatgatt tcaatcttaa tgtacttcgt aatgattact gctgaggtag
     3001 cggtaaaaat ctgtcaactt catggtcaaa acacggttga ccacattgat ttgattgatt
     3061 agctgttggt aatccagatt aactggaccg ataatggcta gaattccaac tccccgataa
     3121 ggaatgagga acttactact aatcaccgct aggtcagcta aacaggactc ttgaccgtct
     3181 gcaatacgaa cattttgcat ctgatcctca cgcaacccct cacgaatctc caaggccacc
     3241 ttttgcggtt ggtcaaagaa ctgataggct gctagattgg caaaattcaa gagattgacc
     3301 ttgcccgcca tcacaatgtt ttcgttgaac atttccttaa agatgtgttc aaagagatcg
     3361 atgacattat ccgttgttgt aaagtaacgc tggataatct gcggaatctc cgtccgaatc
     3421 ttgtagtgaa tatctaaaac ggtgtgaccg aggaaacgtt cctgaatgat gctcttcagt
     3481 ttcagcaaat cctcctgcaa gaagttcctt ggaatcagaa actgactagt aaccgttcgc
     3541 gactcgtcta gggtaaatac cgccaaggct gtatgttgcc ccaaaacaac gatatcaaag
     3601 gctgtcaaac gttgcctgct cggctcaaca tccagtgcca ctaccgtaca gccactcagg
     3661 tctgttagca agttagcagc ctcttgcaga atatcctcca atttgaagaa ttcctgatca
     3721 aaggctttga caatctcata tacctcattt tcagccagcc ggtgaaaatc cagtgagtgt
     3781 ttcacatagt actgaaaacc agcaacactt ggcatccgac cgcttgaagt atgagccttc
     3841 tcaagcaacc cttgtttttc tagttccgcc atgtcattac gaatggttgc actgctagag
     3901 ttaatagact cttgcaaggc ttttgatccg acaggttcgt gcgttttggt aaagatgtca
     3961 ataatcagat ttaaaatatc ctgctgacgc tctgtaacca tctcatcact cctctctgtc
     4021 tgatcaatta gcactcaatg catccaagtg ctaacttatg attctattat acacccttag
     4081 aagagaatgt caagacaaaa actcaaaaaa ttagcactct tttatcaaga gtgctaaaaa
     4141 tcaatctgaa gacctagaac cttgctattc atctactcgg tatttccttt ttagtctgaa
     4201 ataaccatga attagataag aaatcagaaa agcatataaa acaaaaataa gaaagaaaga
     4261 catagaaaga tcctctctaa acaaactcgt acaaacaaca agaccaccta agaaagtagt
     4321 tgtacaggaa cgaagtctag atcgcataac ttcccatttg agccccttac tcatatagac
     4381 ttgatcctct ggaagtaaat cagaagacga tttacgaaga atcgaacaag aacctgctcc
     4441 tatcaattcc caacctctat ctctaaaatc ttgcagttca tgcttatatt ttttaagaaa
     4501 tctagaatca tagatacggt agatgacatc gtctggttga cattggtcaa aatagaacaa
     4561 accaaaacga ctcgttctat acctccaacc tttcaaatgc atctcatgta aatattcttc
     4621 ttccttgtcc aaatcaacaa tggtgaaaat ccgaaattct actctgctat tcattgtctt
     4681 accccaaaat tagaaaacat gcctggcgtt atttattaga taattctttc cacttttgac
     4741 tcaatctcca aaaaatataa gaaatctgaa tcgcaaaaac tatcaataaa acccaatcta
     4801 ttatgaaaat caaaaacact ttccaactga aagaactacc tccagtgaca aactttgagc
     4861 aaaacggtag tagagctaaa aagagaagta aaataggaag catccgcatt gttaaaatcc
     4921 gtttggcata aaaaaatctt tatttaaact aaaatattat agcaaaattt acgccagttt
     4981 ttgaacggct gatgtagata ttttatactt tcaaaatgtt taaatgtgat tatttatttt
     5041 tgaaaaatag ctcaccagcc cgactgaaag tgcttataga atgataataa gtcgcctgcc
     5101 gaaaacagcg gaaaatagcg gtgttatgcg gagataatct gacgcgatgc gaaagtatat
     5161 tgcatactta ttttcaacaa tttagcagag tatttttata agtgtgatat aatagaagta
     5221 taatttgttc tgatagttta ttttatggag aagtagattt ttagaatgcg gagggttcaa
     5281 tatggttgag tttataaagt ctaagaaaga aatgagtgag gaggatatta aagcaaattt
     5341 catcactcct gctattgtat ccaaaggatg gaaaaatggt gagcatatcg cttacgaaga
     5401 atacttcact gatggtcgaa ttgaggttag aggagataag gctcgtcgta aagaaggaaa
     5461 aaaatcagac tattcactgt attaccaatt tggaactcga attgcaattg ttgaggcaaa
     5521 ggataataaa cacagcgttc gagcaggatt acaacaagct attgaatatg gagagatttt
     5581 agatgttcca tttgtttatt cttcgaatgg tgatggcttt attgaacacg accgtatcac
     5641 gagagaagaa cgtgagctgg agttagacga attccctact cgtgaagaat tattttctcg
     5701 tatgacgaag gaaaaaggat tgacgtacga aattacagaa gctatctcaa ctccatacta
     5761 tacagacgcc ttctcaatga acacgccacg ctattatcag caaatagcta tcaaccgtac
     5821 tattgaaaca gttgccagag gacaaaaacg agtaatgttt gtgatggcaa caggaacggg
     5881 gaaaacgttc atggcttttc aaattattca tcgccttcga aaagctggtt tggctaaacg
     5941 agttttattc ttagcagata gaaacatctt agtagaccaa acgatagctg aagactttaa
     6001 gccattcgaa aaggtaatga cgaaaattac accaaaactt ttgactgctc ctgaaaaatt
     6061 aaattctttt gaaatttatc tagggcttta tcagcaacta actggtgaag atggaactga
     6121 aacacattat caaaaatttg acaaagactt ctttgattta atcgtaattg atgaagcgca
     6181 ccgtggttca gctaaggaaa acagtaactg gcgtaagata attgattatt tcagttctgc
     6241 gacacagatt gggatgaccg ctactcctaa agaaaccaag aatgcttcca atacggaata
     6301 ctttggtgag ccaatctata cttatagttt aaaacaggga atcgaggatg gttttttggc
     6361 tccatatcgt gttatgagag ttaatttaga tgtagatgtg gatggttatc gtccagaaac
     6421 tggaaaagtt gatgctaacg gacaattaat agaagatagg tactacggca ggaaagattt
     6481 tgataaaacc attgtcattg atgatagaac gcaaagagtt gccaagtttg tttctgatta
     6541 tatgaagcaa aacaatgcac gatttgataa aacaattgtt ttttgtgttg atattgacca
     6601 tgccgagcga atgcgtgctg cacttgtaaa agagaatcta gacttagtcc aagaagacta
     6661 tcgttatgtc atgcaagtaa ctggtgacaa cgctgaagga aaagctcaac tggataactt
     6721 tatggatgtc aattctaatt ttcccgctat tgtaacaacg tctaaattat taacgacagg
     6781 agttaatgct aaaacatgtc gtttgattgt tttagactct aatatccaat ccatgactga
     6841 atttaaacaa attattggtc gtggcacacg tctttatcct caaaagggga aagaattttt
     6901 tacgattatt gattttcgaa atgttaccaa tttgtttgct gaccctgatt ttgatggtga
     6961 tccagtgaag gtgctggaaa caggtgcgaa aacagtcagt ggttctacgc ccggtttcgt
     7021 aagtgaggaa ggtgacccag tagaaaaata tatcgttaca gacaagcagg ttaccattct
     7081 taattctact gttcaagtat tggatgaaaa cgggaaactg attaccgaaa gcctgaccga
     7141 ctacactcgg aagaatatct taggtagcta cgccactttg aacgatttta tcacagtttg
     7201 gcatacggcg gataaaaaga agcttatctt agacgaactt tataaaaaag gagtttatct
     7261 agatgctatt cgagagtcgg agggaatatc agaacaagaa atcgatgatt ttgatttact
     7321 cctaaaactt gcctatggtc aaaaagaatt aaccaaaacg gaacgtatca ataagctcaa
     7381 acaaagcgga tatttatata aatatagtga ggaagcgcgt gctgttttgg aaattttact
     7441 gaacaaatac atggataaag gtattggaga actcgaaagc attgaaacat taaaacttcc
     7501 agaatttcag atatatggtg gaaccttcaa aatcatcaat acttattttg gagataaaaa
     7561 acgatattta caagcaatta aagaattgga gcaagagcta tttacagtag cttaatgaaa
     7621 ggaaagtatg tcaattacat catttgtaaa aagaattcaa gatatcactc gaaacgatgc
     7681 tggtgttaat ggtgatgctc aacgtattga gcaaatgtct tggttattat tcttaaaaat
     7741 ttatgatagc cgtgaaatgg tttgggaatt agaagaagac gagtatgagt caattatccc
     7801 agaggaatta aaatggcgaa attgggctca tgctcaaaat ggggaacggg tattgacagg
     7861 cgatgaatta cttgattttg tcaataacaa gttattcaaa gagttgaaag agcttgaaat
     7921 aacttcaatt atgcctattc gaaaaacgat tgttaaatca gcttttgaag atgcgaacaa
     7981 ctatatgaaa aatggcgtct tgttacgcca agtcatcaat gttattgatg aagttgattt
     8041 caatagccct gaagatcgtc attcgtttaa tgatatttac gaaaaaattc ttaaagatat
     8101 tcaaaatgct gggaactcag gagaatttta tacgccacgt gcagcgactg attttattgc
     8161 cgaagttctt aacccaaaac ttggagaatc aatggcagac cttgcttgcg gaacaggagg
     8221 cttcttgact tcgactctga accgtttaag tagtcaacgt aaaactagtg aagataccaa
     8281 aaaatataat acagctgttt ttggtattga aaagaaagca tttcctcatc ttttagcagt
     8341 tacaaatctg tttcttcacg aaattgatga ccctaaaatt gttcatggaa atactttgga
     8401 gaaaaatgtt cgtgaatata cggatgatga aaaatttgac attattatga tgaatccacc
     8461 ttttggaggg tcagaattag aaacaataaa aaataacttt ccagcagaat tacggagttc
     8521 tgaaacagct gatttattta tggctgtcat tatgtatcgt ttgaaagaaa atggtcgtgt
     8581 tggagttatt ttacctgatg gttttctatt tggtgaaggt gtaaaaactc gcttgaaaca
     8641 aaaactggta gatgagttca acttgcatac gattattagg ttgcctcata gtgtctttgc
     8701 accgtataca ggaatccata cgaacattct tttctttgat aaaacaaaga aaacagaaga
     8761 aacttggttt tatcgtttag atatgccaga tggttataaa aatttctcga aaactaagcc
     8821 gatgaagtca gaacacttca atcctgttcg tgactggtgg gaaaatcgtg aagagattct
     8881 ggaaggtaag ttctacaaat ctaaatcatt tacacctagt gaattggctg agttgaatta
     8941 taatttagac cagtgtggct ttccaaaaga ggaagaggaa atcttaaatc cctttgagtt
     9001 gattcagaat tatcaagcgg aaagagcaac tttaaatcat aagattgata atgtattagc
     9061 tgatattttg cagttgttgg aggacaaata atgacaccag aacaacttaa agcaagtatt
     9121 ctccaaagag cgatggaagg gaaattagtg ccgcaaaatc ccaatgacga acctgcaagt
     9181 gaattattaa agagaattaa agctgaaaaa gaaaaactta tcagtgaagg aaaaatcaaa
     9241 cgagataaaa aggaaactga gatatttcgt ggtgatgatg ggaaacatta tgggaagttt
     9301 gctgatggaa gcactcaaga aattgatgtt ccttatgata ttcctgatac ttgggagtgg
     9361 gtgaggtttt ctacattggt tgaaattgtc agaggtggct ctccacgacc aatcaaagat
     9421 tatcttactt ctgaagtaga tggaataaat tggataaaaa taggtgatac tgaaaagggt
     9481 gaaaagtata taaataatgt taaagaaaaa atcaaaaaat cagggcttaa caaaactaga
     9541 tttgtaaaaa aaggtacatt tttgttaact aattctatga gttttggtag accttatatt
     9601 ttgaatgttg atggtgcaat acacgatgga tggttggcta tttcgaacta tgaaaactca
     9661 ttaaataaag attacctatt ctatattctt tcatcaaatg tagtttattc tcaatttcta
     9721 tctctaatta gtggagctgt tgtgaaaaac ttgaatagtg ataaagttgc ttctattctt
     9781 atccctctcc ccccactatc cgaacaacaa cgcataatag aagcaatcga atcagcttta
     9841 gaaaaagtag atgaatatgc tgaaagttat aatagactag aacagctaga taaagaattt
     9901 ccagataaac taaaaaaatc tattcttcaa tatgctatgc aaggaaaatt agttgaacaa
     9961 gacccaaatg atgaatcagt cgaagtttta cttgaaaaaa tacgagcaga aaaacaaaaa
    10021 ctctttgaag aaggcaagat taaaaagaaa gatttggaca tttctattgt ttcccaagga
    10081 gatgataact cttattatga ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg
    10141 aggttaaacg atataacctc ctacattcaa agagggaagt ctccaaaata ttcaaatatt
    10201 cctatatacc ctgtgattgc tcagaaatgt aatcaatggt ctggcttttc tattgactta
    10261 gcaagattta ttgatcccga aacagttcat agttaccaga aagaaagatt actaagagat
    10321 ggtgatttaa tgtggaattc aacaggtcta ggtacgttag gacgattagc tatttaccat
    10381 gaaaataaaa atccttatgg ctgggctgtt gcagatagcc atgttacagt tataagagtc
    10441 ttatcgggag ttattaattg ccatttcatc tataattttc tttcctctcc tattgttcag
    10501 tcagttattg aagaaaaagc gtcgggtagt acaaaacaaa aagaattatt aacgaaaact
    10561 ataaaagaat atttaatccc cctcccaccc cttcctgaac aatccagaat cgttgataaa
    10621 atcgaacaat ttttcgccca tattgacgca ctaatttagt ataatagttt tacgtgcggt
    10681 ggaatttcta tcaaacataa gataggagtt ttacgttgaa agattttgaa ttacatttga
    10741 agaaagctgg tttagctgaa aatactgtgc gttcgtacct ttatggtgtt cgatttttct
    10801 tggagaatta cgaactcaaa atggaagatt tatttgaata caaaggatat ttattagaca
    10861 attttaaacc taaaactgtc aatttgcgac tacaaggagt aaataaatat ctggctttta
    10921 ttggtcatga tgatttgaaa ttaaaatttg tcaaagtaca gcaaaaacca tttttagaag
    10981 atgtcattag ccatgctgat tacctttttc ttaaacgtag tttgaaaaaa gatggtattt
    11041 taaaatggca ttttgtcgtt tggtttttag gtgcaactgg ggctcgtgta agtgaactta
    11101 ttaaactaaa agtagagcat gtagaaatcg gttattttga catttactca aaaggtggta
    11161 aaattcgtag actgtacatt cctaaaaaat taagaaatag ttgtcttagt tggttagaat
    11221 cagaaaatcg ccgaagtggt tatttatttt tgaataaatt taacgaacca attacagcaa
    11281 gaggagttgc tcaacagtta aaaaattatg ctgataaata caaaatgaat cctaaagtaa
    11341 tttaccctca ttcttttagg catttatttg ctaagaattt tttagcgaag tataatgata
    11401 ttgccttgct tgcagatttg atgggacacg aaagtataga aactactcga atttatctaa
    11461 ggaaaacagc tactgaacaa caaaatattg tagataaaat tgttaattgg taaaaaataa
    11521 caggtggtca aactgactac ctgctatttt tgtgattatg
//
LOCUS       DAVLSL010000055        11549 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLSL010000055 DAVLSL010000000
VERSION     DAVLSL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.056"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(130..444)
                     /gene="trxA"
                     /locus_tag="VXN30_001142"
     CDS             complement(130..444)
                     /gene="trxA"
                     /locus_tag="VXN30_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1357771.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(461..682)
                     /locus_tag="VXN30_001143"
     CDS             complement(461..682)
                     /locus_tag="VXN30_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1357772.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(781..1161)
                     /locus_tag="VXN30_001144"
     CDS             complement(781..1161)
                     /locus_tag="VXN30_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1357773.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(1305..2258)
                     /locus_tag="VXN30_001145"
     CDS             complement(1305..2258)
                     /locus_tag="VXN30_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1357774.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            complement(2326..3534)
                     /gene="sstT"
                     /locus_tag="VXN30_001146"
                     /pseudo
     CDS             complement(2326..3534)
                     /gene="sstT"
                     /locus_tag="VXN30_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            3673..4233
                     /locus_tag="VXN30_001147"
     CDS             3673..4233
                     /locus_tag="VXN30_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1357775.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            4290..5198
                     /locus_tag="VXN30_001148"
     CDS             4290..5198
                     /locus_tag="VXN30_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1357776.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            5150..5740
                     /locus_tag="VXN30_001149"
     CDS             5150..5740
                     /locus_tag="VXN30_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1357777.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIDIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            5743..6849
                     /gene="yqeH"
                     /locus_tag="VXN30_001150"
     CDS             5743..6849
                     /gene="yqeH"
                     /locus_tag="VXN30_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1357778.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            7063..7374
                     /gene="yhbY"
                     /locus_tag="VXN30_001151"
     CDS             7063..7374
                     /gene="yhbY"
                     /locus_tag="VXN30_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1357779.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            7426..8055
                     /locus_tag="VXN30_001152"
     CDS             7426..8055
                     /locus_tag="VXN30_001152"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1357780.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            8055..8648
                     /gene="yqeK"
                     /locus_tag="VXN30_001153"
     CDS             8055..8648
                     /gene="yqeK"
                     /locus_tag="VXN30_001153"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1357781.1"
                     /translation="MAYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRSFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            8649..9149
                     /locus_tag="VXN30_001154"
     CDS             8649..9149
                     /locus_tag="VXN30_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1357782.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFVGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            9162..9515
                     /gene="rsfS"
                     /locus_tag="VXN30_001155"
     CDS             9162..9515
                     /gene="rsfS"
                     /locus_tag="VXN30_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1357783.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            9618..10364
                     /locus_tag="VXN30_001156"
     CDS             9618..10364
                     /locus_tag="VXN30_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1357784.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKITFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            10374..11471
                     /locus_tag="VXN30_001157"
     CDS             10374..11471
                     /locus_tag="VXN30_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1357785.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKVDQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         3459 a         2269 c         2628 g         3193 t
ORIGIN      
        1 ttctaacaat gttttagaag tagaggtgta ctattctagt ttcaatctgc tatagtctag
       61 catatttttt gaaaaatggc aaagggcaag aaaaaagaga ccaaagaaag tacttggtct
      121 ctcgtttgat tagctcaatt cagcaatgat ggccttgatt tgttctgctg tgtgaacacc
      181 tgcaacctgt ttgacaactt ggccgtcttt tttgaagaga agagttggaa tagacatgat
      241 tccaaaagca cgagctgtgt ttggattttc atcaacgtcc attttaacga ttttcaagac
      301 atcttctgaa agttcttcag acaatttgtc caagattgga ccttgcatac gacatggacc
      361 acaccaagtt gcccagaagt ctactaagac caaaccgtct tttgtttctt gttcgaatgt
      421 tgcatctgta attgcttttg ccattgtatt tctccttttt ttagttatat tggcttaaat
      481 cttgtttcat gagatagaag aagatatctc cataagtccc atggtagtcc aaattatggc
      541 ccttgtaagt taatttttgg acagggtagt agtctgcgac gccgataagg caagcttgtt
      601 gcgaacgttc aaagtcttca taagactcga aagtcacagt tctttcgttc ttgctggcat
      661 ctatataggt aatttcaatc atatttaaaa ctcctttgtt taatgctaac tttattttac
      721 tccttataaa agagaatgtc aagaaaaatg attgcgcacg caactttttt taaaatcatc
      781 ttaaatcaag aaatccaaac ctgcttccaa gctttcttcg acagtctttt gtagcgaggc
      841 cagtgtcttt tgcccatcat ttgtcaggca gataaaacta gagcgtctat cttgatggca
      901 acacatgcga ctgagtagac cgcaattttt agcttccaag cgagccacca tcctagaaac
      961 tgcgctcggg ctcagatgaa gcttatctgg caggtcaatc tggcgtagag atttttcttc
     1021 agccaagtcc agatagtaga gcaggtagaa ctctttcaag gtcagacttt gctcgctctg
     1081 ttgggcaatg gtctcttcca agagactttc aatttctttc tgacgccgat tgaagtcaaa
     1141 ccatttttcc aaataggtca tagtgtctcc tttcttttta gagtcataaa atagaagaaa
     1201 gtccattaac gggcagtctc tgcgtcacaa gatgattgcg catgcaataa ttatactact
     1261 tttcaagaat gctggcaagc tctgtttttt agtggtttta ttttttactg tccatatttt
     1321 tggtaaaaat caacttttac ttggatgaag gttttggctt cacgtaggag ttgaagaagg
     1381 gtggcgcggg tttcaaactc ttctcttgtc ttgggcagac tgcggttccg gaagacttcc
     1441 agataacgtt caatttcatc tagcaaatca gaagcaggat tggtctggct cagttgacct
     1501 gcaatttttg aaaagagttg cgctaagatc aggctttcac tggcggcaag gtgacaagtg
     1561 ttaatctgtt gggccatgtt tctcaggata cgactttgtc gttgtctcat ctcaaagtag
     1621 tggatatggt agtcggtctg gtgaaagagg tggtcagagt gatccaaata gaccagtctg
     1681 agggcttctt tcaaaagcgt gtctaattct gctaccagct gtgctcggtt gcgtccgtct
     1741 cctctggata aatagtattt gaagcgctgg aggatatctt ttaacttttc ttccaccagc
     1801 gtgtggtagt gctggatttc ctcttctcgt gaaggcatat agagattaac aagcaaggca
     1861 aatcctgtac caatagcaaa gagaaggaat tcattgacta gaaggtctgg agaggttgac
     1921 tcttgaacca agagatggct aaccaaaaca gtgcttggtg tgatgccaat ttcccagccc
     1981 atcttgtagg ctaaaggaac gtagaaggcc agatagaggc cgagactcca gatatgaaat
     2041 ccgctcaagt gaaaagctag aacaccgata gccagagcta gaagcataga aaaaagacga
     2101 ttgcgagcca gttttaaagt acttctacgc gtatcagata ggctcaagag agcgataatt
     2161 ccagccgaaa ctgctgatga aagattgaga aaataagcaa gcaggcaggc aagacaggta
     2221 gctaagatga gcttggtcgt acgttggcta atagacataa gaatttccta ataagttaga
     2281 ataaaagcgt aaaagacaag acatgagcag gcttgccttg atgagttatt ttttacgggt
     2341 tgctgcgtat tcggcaacgg cggtaaagag gacatctgta gaagagttaa gggctgtttc
     2401 acatgagtct tggatgacac caatcacaaa accaacccca acaacttgta tggcaatatc
     2461 gttagaaata ccgaaaaggc tacaagcaac tgggataaga aggagggaac ctccggcaat
     2521 acctgaagca ccacaggctg agatagctgc taccacactg aggacaaagg ctgtggcaaa
     2581 gtcaacagga attccaagag tgttaactgc agcaagggtc aaaacgttaa tggtaatcgc
     2641 tactccagcc atattgatag tagaaccgag tgggatagaa acagaatagg tatctgggtt
     2701 gagtccaagg tcatggcaga gtttcatgtt gacaggaatg ttagccgcag aactacgagt
     2761 gaaaaaggct gtcacaccgc tgacacggag gcagttccaa actagagggt aaggattacg
     2821 tctcataaag aagaaggcaa tcaaagggtg accacaaggg caacaaaaag catagtcgtt
     2881 actaatagaa ccaataaaat accgtagttg gcaaggcttc cgactccctt gtcagaaatg
     2941 gttttaaaaa caagaccaag gcttccaaat ggagccagat tgatgatcca ttcgacaatt
     3001 ttagaagtca cgtcagcgat agtttttagc aattctttac tatttttact ggcttctctc
     3061 atagcgattc caaaaatgac tgcccaagat aagattccaa tatagttagc agtaaacagg
     3121 gcgttgactg ggctgtcaac cagtttgagc aagaggttgc tgagaacctg cccaacccca
     3181 tctggtggtg caatttcagt attggcacta tttagggtaa tttcaatagg gacgatgaaa
     3241 cttgctagta cagctacaag agcagcggcg aaagtcccta tcataggata tacaagaaaa
     3301 caacagtttt catattgcta tcttgtccct tttgatgttg ggaaagggca ttggcaacga
     3361 gggcaaagac taggatagga gcaacagctt ttagacctcc aacgaataaa tccccgagta
     3421 gcccaatccc tgagagatta ggaagggtca gtcctaggat tccccacaaa gcataccaat
     3481 caagatacgc ttgacaaggc ttgccttatt ccaagcatga atgattcttt tcataataat
     3541 ctcctttttg tgtagtgatt atgattatag tataaatgat agacaaaatc aagaattttc
     3601 tgtctatttt ttgaatattt atggagaatg agactgatga aaatatggta taatgaaata
     3661 aaggagtttt atatgcaaaa atttattcag gcttatattg aaaagctaga tgtgacaacc
     3721 attatcgaga atattctaac caaggtcatt tctcttttac tgcttttgat tgtattttat
     3781 attgctaaaa aaatgcttca taccatggtg cagagaattg tcaaaccttc tctaaaaatg
     3841 tctcgtcatg atgtcggacg ccaaaaaacc atctcacgtt tactagaaaa tgtgtttaat
     3901 tatacgctat atttcttttt actctactgc attttgtcta ttttaggttt gccagtttct
     3961 agtttgctgg ctggagctgg tattgctggg gtagcgattg gtatgggagc ccaaggcttt
     4021 ctgtctgatg tcatcaatgg ctttttcatc ctctttgaac gtcaactgga tgtgggagat
     4081 gaggtcgttc tgacaaatgg accgattact gtatcgggta aggttgtcag tgtgggaatt
     4141 cgtacgacac agctgcgtag cgaggagcaa gcccttcact ttgtccctaa ccgaaatatc
     4201 acagttgtta gcaatttctc acgcacagac tagacctgtt attttaagta atttgtggta
     4261 caatagaggg agtttaataa ggagaaaaga tggttttaga aaagcagttg ggcaatggtt
     4321 gtacctggat agacctagac ctaggaaagt tgaataaact agaagacctt tctgaaattt
     4381 acggtttgga caaggaaacc attgaatacg cactggatag aaacgagcgc gcccacatgg
     4441 actaccaccg tgaaagtggg acggttacct ttatctataa tgtcctagac gtaaaaaagg
     4501 acaaggccta ctatgagact tttcccatga cctttatcgt cgagcatcgt cgcctgatta
     4561 ccattagtaa taccaagaac gcctatgtca ttgaacagat gactcgttat ctggagagcc
     4621 atgacacgct ttcgatttat aagtttctct ttgccagtct ggaaatcatc agcaatgcct
     4681 actatcctgt cattgagcag atggacaaga gtagggatga ggtcaatgac ctcttgcgcc
     4741 agcgaactac caagaaaaac ctctttgccc tgtctgattt ggagactggt atggtttatc
     4801 tgacggcagc tgccaaacaa aatcggattt tgttagagca tattcaaggt catgccttgt
     4861 atcgtagttt tgatgagatt gagagagaac agtttgatga tgctatgatt gaggctcatc
     4921 agctggtatc catgacagac ctaatctctc agattttaca gcagctttca gcctcttaca
     4981 acaatattct aaacaataat ctgaatgaca atttgacaac cttgactatc atttcagtct
     5041 tgctagctgt tttggcagtc gtgacaggct ttttcggaat gaatgttccc ttacctttaa
     5101 cagatgagcc ccatgcttgg ctctatatca gtttggctag tgcaggtttg tggattgttt
     5161 tatccttgtt actaaggaaa attgcgaaaa aaagttaaga aaaggagcca gaatggcgat
     5221 tgaaaattat ataccagatt ttgctgtgga agcagtctat gatctgacag tcccaagcct
     5281 gcaggcgcag ggaatcaagg ctgttttggt cgatttggat aataccctca ttgcttggaa
     5341 caaccctgat ggaacgccag agatgaagca atggctacat gatcttcggg acgcgggtat
     5401 tgacattatc gtagtgtcaa ataacaccaa aaaacgcgtt caacgagcag ttgagaaatt
     5461 tgggattgat tacgtttact gggccttgaa gcctttcaca tttggtattg accgtgccat
     5521 gaaggaattc cactatgaca aaaaggaagt ggtcatggtt ggcgaccagc tcatgacaga
     5581 tatacgagca gcccaccgtg cagggattcg gtcaatttta gtcaaaccct tggtccaaca
     5641 tgactcaatc aaaacgcaga ttaaccgaac tcgtgagcgt cgtgttatga gaaaaatcac
     5701 tgaaaagtac ggaccgatta catataaaaa aggaatttaa ctatggaaga aattctctgt
     5761 attggttgtg gagcaaccat tcagacgaca gataaggctg gtcttggttt taccccccag
     5821 tcggcacttg aaaaaggttt ggagactggc gaagtctatt gccaacgctg tttccgtctc
     5881 cgccactaca atgaaatcac agatgtccag ttgacgaacg atgatttcct caagctcttg
     5941 cacgaggtgg gagacagtga tgctttagtg gtcaatgtca ttgatatctt tgattttaat
     6001 ggatctgtca tcccaggttt accacgtttc gtctcgggca atgatgtcct cttggtagga
     6061 aataaaaaag atatccttcc taagtcagtt aagtctggta agattagcca gtggctcatg
     6121 aaacgtgccc atgaagaagg tcttcgtcca gtcgatgtag tcctaacttc agcacaaaat
     6181 aaacatgcca ttaaggaagt cattgataag attgaacact accgtaaggg ccgcgatgtc
     6241 tatgtggtcg gtgtgaccaa cgttggaaaa tcaactctaa tcaatgctat tatccaagaa
     6301 atcacgggtg atcagaatgt catcactact tcacgctttc cagggacaac cttggacaaa
     6361 atagagattc cgcttgacga cggatcttat atttacgata cgccgggaat tatccaccgc
     6421 caccagatgg ctcactactt gacggccaaa aacctcaagt atgtcagtcc taaaaaggaa
     6481 atcaagccta agacctatca gcttaatcct gagcaaaccc tatttttagg tggtttggga
     6541 cgctttgact ttatagcagg agaaaagcaa ggatttactg ctttctttga taatgaactc
     6601 aaactccatc gtagcaagct tgaaggagct agtgctttct acgataagca cctgggaact
     6661 cttctgacac caccaaatag caaggaaaaa gaagatttcc caaggctagt ccagcatgtc
     6721 tttaccatta aagataagac agacctagtc atctcaggct taggctggat tcgtgtaaca
     6781 ggcacagcaa aagtcgccgt ctgggcacca gaaggcgtcg ccgtcgtcac acgaaaagca
     6841 attatttaaa acaaaatcgc tgagcgaaca aagtgagctc atatagcgat agttattgcc
     6901 gtggtgtgat tgccaatctg tagattggca aggggcacaa ttgagaccaa ggctcaattg
     6961 tgaggtcagg aaatccattt ttcaaagatg aaatctttgg aaaattagtt ccgaccgtcc
     7021 ctcaccacct aaaataacta tccaaaaaca aggaagaaaa ttatgtcatt aacatcaaaa
     7081 caacgtgcct tcctcaacag ccaggcacac accctcaaac ctatcatcca aatcgggaaa
     7141 aatggactca acgaccaaat caaaaccagc gtccgtcaag ctcttgatgc gcgtgaatta
     7201 atcaaggtta ctctcttaca aaacacagat gaaaacatcc acgaagtagc tgaaattttg
     7261 gaagaagaaa tcggtgtgga tacagtccaa aaaataggac gcatcttgat tttgtttaaa
     7321 caatctagca agaaagaaaa tcgcaagatt tctaagaaag tcaaagaaat ctaagaccga
     7381 aactccaaat aactgttttt atagagaaat aaaggggact agcctatgac aatcgaacta
     7441 ttgactccct ttaccaaggt agagttggag ccagaaatca aggagaaaaa acgcaaacaa
     7501 gttgggattt taggggggaa ttttaaccct gttcacaatg cccatctcat tgttgcggat
     7561 caagtacggc aacagttggg actggatcaa gttctgctca tgcctgaata ccaacctcct
     7621 cacgttgata aaaaggaaac catccctgaa caccatcgtc tcaagatgct tgagttggca
     7681 attgagggga ttgacggcct agtcattgaa accattgagt tggagcgcaa gggtatttcc
     7741 tacacctacg acaccatgaa aatcttgaca gagcaacatc cagatacgga ttattacttt
     7801 atcatcggtg ccgacatggt tgactatttg cctaagtggt accgaattga tgaactggtt
     7861 gacatggttc agtttgtggg ggttcagcgt ccacgctaca aggcaggaac ttcctatcca
     7921 gttatctggg tggacgtacc gctcatggat atctcgtcca gcatggtgcg tgccttcctt
     7981 gcccaaggtc ggaaacccaa ctttctccta cctcagcctg tgctagacta tatcgagaag
     8041 gaggggctct actgatggcc tatcaagact atatcaactg ctctcgtgag gccttgttgg
     8101 aaaaaatggc agagcttcta cctgagaaac gtctaaccca ttgtctaggt gtggagcgtg
     8161 ccgctatgga attagctcag cgctttggtg tagatgttga gaaagcaagt ctagcaggtc
     8221 ttcttcatga ctatgctaaa aagttgtcag atcaggaatt tctagacttg attgaccgtt
     8281 atcagctaga tttcgacctc aaaaactggg gtaataatgt ctggcatggt atggtcggaa
     8341 tttacaaaat tcaggaagat ttggatttgc atgattcaga aatcctgcga gccattgaag
     8401 tccatacagt cggtgctggt cagatgacag acctagataa ggtcatctac gtcgcagact
     8461 atatcgagca caatcgatcc tttcctggag tagatgtggc gcgtgagatt gcaagtctat
     8521 cgctcaataa ggcggtggcc tacgaaacag ctcgtaccgt ggagtatcta gctcatcagg
     8581 gattccccat ctatccccaa acccttgaaa cctataacgc ctttgtgcac tatttgaaag
     8641 agaactaaat gaagacggct tttataatca ttgatgttca gaatgtttta gtagaaactg
     8701 ggttccagac aaaaagtcta ttgggaaaaa tttcttattt acaaaaccag gctagaagca
     8761 agaatattga aattatctat gttcaacata ttgagaactc tgaagctcaa acatcagaag
     8821 attggcagtt atctgcgctt ttaaatcgaa aacctgctga aaaggtcttt cagaagaagt
     8881 ataacagtat tttcaaagaa actggcttaa aagaatactt ggataagcag gggattgaaa
     8941 aattagtttt atgtggtatg cagacagaat attgtgtgga tacctctgtc aaggttgcct
     9001 ttgaatatgg ctatcagctt attgttccag aaggtgctgt cacaaccttt gttggggatg
     9061 acattccagc agaaacgatt aatgaatttt atgaggacat ttgggaggag cactttgcag
     9121 atgtcctaga ttacaaacat attttctaaa aagaggatta aatgaacgaa aaagaattac
     9181 tagaactagt cgtgaaagcg gctgatgaga aacgtgcgga ggatatcctc gcacttgatg
     9241 tacaagattt gactagtgtg acggactact ttgtcatcac tagctcaatg aatagccgtc
     9301 agttggacgc tatcgcagct aatatccgtg aaaaagtagc tcaagcaggc tttaaaggta
     9361 gccatgtcga aggcgatgca gctggaggct gggtcttact ggacctcggt gctgttgtcg
     9421 tgcatatctt ttcagaagaa atgcgtgccc attataacct agagaagcta tggcatgagg
     9481 cgaattcagt agatatttca gaagctcttg cttagaagat gaactcagtt gtagtcaact
     9541 gggtttcttt gcttatttta gaaaaattga tagaaaggta aagggctagt ggtgtttacc
     9601 atggagctct tagtatcatg attatggcga cttatgaaac ctttgcggcg gtctatgatg
     9661 cggtcatgga cgatagttta tacgacaaat ggacgaattt ttctctgcgt catttgccta
     9721 agaccaagga gagaaagaaa ctcttggaat tggcttgtgg gacaggaatt caatcagtgc
     9781 gcttctctca ggctggtttt gatgtgactg gacttgactt gagtgcggat atgttgaaga
     9841 ttgcggagaa gagagcaact tcagccaagc aaaagattac ttttatagaa ggtaatatgc
     9901 tgaatttgtc caaggcaggg aaatacgatt ttgtcacgtg ttattcggac tctatctgct
     9961 atatgcagga tgaggtggaa gtaggggacg tctttaagga cgtgtacaat gcgctgaatg
    10021 aagagggagt tttcatcttt gacgtgcact cgacctacca gacagatgaa gtattccctg
    10081 gctattccta ccatgaaaat gcggaagatt ttgccatgct ttgggataca tatgaggacg
    10141 aagctcctca ctccatcgtg catgagctga ctttctttat caaggaggct gacggttcct
    10201 ttagtcgcca cgatgaagta catgaggaga ggacctatga gatcttgacc tatgatatct
    10261 tgctggaaca ggctggattt aagtccttca aactctatgc agactttgag gataaggaac
    10321 caacagaaac tagcacccgt tggttttttg tggcgcagaa gtaggagaac atcatgacca
    10381 tcacaggtat tatcgcagag ttcaatcctt ttcataatgg ccataaatac ctgctggatc
    10441 aggcagaggg actgaaaatt gttgccatgt cagggaattt catgcagcgt ggagagcctg
    10501 ctattgtgga taagtggaca agggcccaga tggcgctgga aaatggcgca gacttggtag
    10561 tggaattgcc ctttttagtc agtgttcagg cagctgattt ctttggtcaa ggagctgtgg
    10621 atatcttgga tcgcttgggc attgatagtc tagtttttgg gacagaggaa gttcgagatt
    10681 atcagaaaat cgctgactta tacacagaga aaggtactga gatggagaaa tttgtggaaa
    10741 atctgcctga ttccctttcc tatccccaga aaacccaagc tatgtggaag gaatttgctg
    10801 gtcttgattt ttcaggtaat acccccaatc atgttcttgc tctggcctat gcaaaggcgg
    10861 ttgcaggacg aaatatcaag ctgcatccga ttcagcgtca gggggcaggt taccattctg
    10921 tgaataaaga tgtggacttt gcctcggcga cagccctccg tcaacatcag aaggaccaag
    10981 atttcttaga acgttttatg ccttctgttg ccctatttga gcaggccagt aaggtgatct
    11041 gggaagacta ttttcccttg ctccgctatc aaatcttgtc aaatcccgac ctaaccacca
    11101 tctatcaggt caatcaagaa atggctgtgc gcattaaaga agctattaaa acagcccagt
    11161 ctgtggaaga attggtcgag ttagtgacta ccaaacgtta caccaaggcg cgtgtcagac
    11221 gcctattgac ctatattttg gtacaggcta gagaaaatgt cttgccagaa gccattcatg
    11281 tccttggatt tactgaaaaa ggcaggcaac atctcaagtc tctgaaagga caggtcaatc
    11341 tagtcagccg aattggcaaa gaaccttggg atgctatgac tcaaaaggtt gaccagattt
    11401 accaactagg aaagccaagt atagcagagc aaaattttgg cagagtgccg attagaatag
    11461 aaacaaacta agtctactga aaggtaggct tttttattgt agaaatttta gataacaaat
    11521 tgaagataaa gtttttggaa aatgataaa
//
LOCUS       DAVLSL010000056        11379 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLSL010000056 DAVLSL010000000
VERSION     DAVLSL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.057"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(163..309)
                     /locus_tag="VXN30_001158"
     CDS             complement(163..309)
                     /locus_tag="VXN30_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357786.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            complement(306..761)
                     /locus_tag="VXN30_001159"
     CDS             complement(306..761)
                     /locus_tag="VXN30_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357787.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            complement(898..1782)
                     /locus_tag="VXN30_001160"
     CDS             complement(898..1782)
                     /locus_tag="VXN30_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1357788.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(1786..2172)
                     /locus_tag="VXN30_001161"
     CDS             complement(1786..2172)
                     /locus_tag="VXN30_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1357789.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(2165..3508)
                     /locus_tag="VXN30_001162"
     CDS             complement(2165..3508)
                     /locus_tag="VXN30_001162"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1357790.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRFFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(3590..4474)
                     /locus_tag="VXN30_001163"
     CDS             complement(3590..4474)
                     /locus_tag="VXN30_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357791.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(4486..5103)
                     /gene="cysE"
                     /locus_tag="VXN30_001164"
     CDS             complement(4486..5103)
                     /gene="cysE"
                     /locus_tag="VXN30_001164"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1357792.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(5119..7332)
                     /gene="pnp"
                     /locus_tag="VXN30_001165"
     CDS             complement(5119..7332)
                     /gene="pnp"
                     /locus_tag="VXN30_001165"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1357793.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDIIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            complement(8017..8883)
                     /gene="metF"
                     /locus_tag="VXN30_001166"
     CDS             complement(8017..8883)
                     /gene="metF"
                     /locus_tag="VXN30_001166"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1357794.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQYMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     NAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8947..11196)
                     /gene="metE"
                     /locus_tag="VXN30_001167"
     CDS             complement(8947..11196)
                     /gene="metE"
                     /locus_tag="VXN30_001167"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1357795.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSFVAAYQEVFAKLADLGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLFQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGKNAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
BASE COUNT         3196 a         2551 c         2256 g         3376 t
ORIGIN      
        1 gtgactatac ttttcatatt tataattcat tcctttcgtt tcactcaagg cacaacacag
       61 aatgaaaaag tgttgtgatc tttattttgt tttataataa tagtgagaaa gcctatcact
      121 actacaaatc acggggaggt gaataagtga gtggtacagc cactacctcg catattttgt
      181 cacatcattt aacggtacat aataagttgt accatctgaa taagttgcta caatatcatt
      241 tgcatgctct ccttcacctt tagcaaaggt tggagctcct gctggatgat ttttatttgc
      301 ctctttcaat ttttcaataa tggcattttt tctgtatctt ttatattatc aggatttttc
      361 actaagattt tgtctggata tgtcggttta gcagaaacaa tttttactgt tacttctttt
      421 ttattcgaag cacttgtcca gtttccagca ttatctttag catttaattt tacagtaatt
      481 cctgaactag gaacttcagt agcaggttga ttatcaacat tattcaactt taatttcaaa
      541 agagctgttg catcagacgt tttatcaatc gttatatata atgatgaatt gttattataa
      601 acagttcctt catatttagc tgtttgtgag ctacttgaaa cagaactgaa attataccca
      661 ctacctccct gattatcttc aatgcttacg tctaaatgaa cttccccact attatttggc
      721 ttagcaacaa ctgttatagt aaaataacat aaaatttgca taaatagatt agggaaatca
      781 aagcagcttc taggaatgtt ttagcagtca cagtgtactt tcccagcatc aagccactat
      841 aactctgcac ataaaaatgg agaagatggc aatcctcttc tccaaatatt aacttcttta
      901 caaaccaact atagttgaca aagaacctaa aatcaattga taacacaagg tcaggtcggt
      961 caactctttc aactgaagcc ctgtcaactc ttcccattta tcaatcttgt attggagaga
     1021 attgcggtgc agatagagtt gctgggctgt tttagtgaga acagcactat tttcccaaag
     1081 agagagaatg atttcctgaa tctgatcttg atccaaaatc atctggtgta gacattcctt
     1141 gattggcttc aagtccacga gtctttctcc catactccaa agatagagct gagaaaaagt
     1201 atgaacacct tggtgaccct gacgccacca tgtcttgaac aaatcccgct cagctttgat
     1261 taagtctgat agggcttgat gtcccgtctg agaccaaacc tgacccaaca tgatagaaag
     1321 acgaagtcca aagtcatact caaccgcttc aatcgtatca cttaaaatat ctcttacaga
     1381 agtgtatttg tcttgttgaa gcacgaaaac ataatcctga gctccgacct gtagcactgt
     1441 ctgacaattc ggaaaaagag tccgcatcat atctagccaa gaagccagat tttcctgctg
     1501 aaaataagaa agatggcaat aaaccaactg aatcttttta aaaacttgcg gtgcctgtcc
     1561 cttgccctca accagatagg aataccaagg gtttagcgaa cgagcctgct cctgctgggt
     1621 caaaagggca accaactgct tttcacgctc gctgagccca gcttcctcca gcaaaatcca
     1681 ctgctgagaa gctaaaggga gcgtgagata gccctctttc tctactggtt ggtctgaaat
     1741 ccgagcctca ggaaaccagt cttgtagttc ttttgccctc atgttctagc cctccacttt
     1801 ttggatgcac catgaaacca aactctcaag acgttccaga ttctcagtca tatggagata
     1861 gcccataacc gcttcaaatc ccgtggacat acgataagtc acgacatctg catttttagc
     1921 ctttgtgtgg ctattggtat tgcggccacg tttgtagatt tcttcttctt tttccgttag
     1981 gacctgctcc tccaacatga gagcaatcag gcgagcctga gccttggctg acacgtactt
     2041 agttgcttct tgatggagtt tattgggttt ggtcatacct ttgaggatga ggtgacggcg
     2101 aatatacata gaatacaccg catccccctc aaaggctagc gcaatcccgt taatgagatt
     2161 gacatcaatc acgtgtccac ctcactccat ccttggtatc aaggagctta attccttgag
     2221 taaccaattg gtcacggatt tggtctgctg tcgcaaagtc acgattggca cgcgcctctt
     2281 ggcgtttttg aatcaagtct tcaatctctg catccaaaac ttcctcaaca aagacaattc
     2341 caaaaatttc taacatatct gcaagagctt gcttgacact tgcatcatag ttccctgagt
     2401 tgatccattt ggccatttca aagacaactg tgataccgtt ggcagcatta aaatcttcat
     2461 ccatagctgc tacaaactta tctttaaagt tttgtaactc ttgggcatcc acatttcctg
     2521 taaatggttg ttcgtaagta ttcttgagat acttgagatt ggtctcggca tcgcgaactg
     2581 ccttttccgt gaagttgata ggcttacggt agtgctgggt cgcaaagaag aaacgaagta
     2641 cttgcccatc aagagtttta agggcatcgt gtaccgtaat gaagttaccc aaggacttag
     2701 acattttgac attgtcgata ttgacaaagc cattgtgcat ccagtagtta gcaaaagcct
     2761 tgcctgtttt agcttcagac tgggcaattt cattggtgtg gtgtggaaac tctaggtcag
     2821 ctccaccacc gtggatatca atggtatcac ctaaaatctc tgtcgacatg actgaacact
     2881 caatatgcca acccggacgt ccaggtcccc aaggactatc ccaagaaatc tcacctggtt
     2941 tggaagattt ccatagagca aagtctacag gattttcctt acgagccgtt tcttcatcgg
     3001 tacgacctga agcacctagc tccaaatctt ccaaggtttt attagccaat ttagcatagt
     3061 tgtgggattt ttctacacgg aaatagacat ccccttgact ctcataggca aagcctttct
     3121 cgatcaagtc ttccacaaaa cggatgatgt ctgccataaa ctccactaca cgcggatggc
     3181 gagtcgcagg tttcacgccc aatgccgtca catcctcacg aaaggcagcg atgtacttat
     3241 ccgcaacctc ctgaggcgtg ataccttctt ccctggcacg gttgataatc ttatcatcca
     3301 catctgtaaa attggaaata taggcaacct tatacccacg gtactcaaaa aagcgacgaa
     3361 tcgtatcaaa agctaccgtc gaacgggcgt ttcctacgtg gatatagttg tacaccgttg
     3421 gcccacaaac atacatcttg atcttgccgt cctcaatcgg gacaaattct cgcaaatcac
     3481 gagacatggt gtcatagatt ttaatcataa atcataatca ggaaagctga aatccaagaa
     3541 caattagttt catcactaaa agttcaagta aatttcagtc cgaatatctc tacacttcgg
     3601 aatcccttgc tcctttctca ttcagataaa ccacctgagt ctgtttgaca aagccaattt
     3661 tttcatacaa acgtttggca cctacattgc tatcttccac tgcaatctga aattccttgt
     3721 cattttgctc aattagttgg ttgacgaggg attttgctaa gtagcttcca tagccttttc
     3781 cacgttcagg ttccaatatt gctaaaccgt agaggtaatt cgtattagtc gataaatcaa
     3841 ccgtacaagt tccaataacc tgaccagctt ttaataaaat atatagtcgg ctttctggat
     3901 ctttcagagc ttcagcgaca tatctatcca caacttctct cgattcatgt tcctctgaaa
     3961 atgcctgaaa ttttaattga ctaatttgat cctgatacga actatctgct aacaaaactt
     4021 caagatggga aacatttgct aacggataag gtcttctatc cttacctaac caagtttctg
     4081 tctcttcatc ctcgattagt ccccagttac tggcaaagtc aggatgattc tctaaaaaaa
     4141 tacgttctgt ctgaaaagtg actgaccgaa tggggaaaga agctgtttct ctctcaaaac
     4201 tagtaaacaa tgcacgcgca atcccctgac ggcgatgacc tggatgaacc agtatcgtca
     4261 cttctacatc ttggtcatct gcatagacag ttaataaacc aacaagttcg cctttttcat
     4321 aataaaggaa aaaggcgggc atgtttgggt caaaattaag catgttagag agatagggat
     4381 cgcgataggt accgtcatag ttttggcaac agttaattac ttttttcgcc tcagatagct
     4441 cctcttggct taacttgttt cttgcttgaa tcatataggt atcctctaca aaccagacga
     4501 tctgtgactg gcatctttag cctgctcgag tttattgaca taatactctc gtttttcttc
     4561 gacttcgtga atgacaggct catctttctt accatgaaga cggacaatct tggccggaat
     4621 accgacaacc gtcacgtcac taggtacatc tgctacgaca actgctgcag caccgacctt
     4681 ggcattttca ccaatttcca caggcccgat aacttgggca tgggctgata tgagggctcc
     4741 ctttcgtaca gtcggatggc gtttgccaca gtctttccct gttcccccga gagtcactcc
     4801 gtgatagaga agaacgcctt tttcaacaat cgctgtctct ccaatcacca gaccagaacc
     4861 atggtcaata aaaacacctg aatcaatctg ggctcctgga tgaatctcaa tctgagtcca
     4921 aaagcgccaa aactgactgt acatacgagc taatagtttg aagccgtgct tccagagaaa
     4981 atgcgagaga cggtgggccg ccaaggcctt gacacctgga taagtcagca aaacctccaa
     5041 agtggtgcgg gccgctggat cattttcttt tacaatatca atggtttcgc gccaccaccc
     5101 catacatttc tccttttctt attctgaatc ttttgatgtt tctgtaaatt ctttcttagg
     5161 tttgtaatcc ttttgatgac gtgggcggtg agggcgctca gacttttcac ctttttcatc
     5221 atgctcaggt tttggcggac gaggtagaag agccttcata gaggcatcga tacggccttt
     5281 ttcatcaatt ttgataacct taacatcaac ttcatccccg atttctacca aatcctctac
     5341 acgattggta cgagtccaag ccatctcaga gatatgaaca agggcatctg tcttatcaaa
     5401 gaggttaaca aaggcaccaa atttctcgat acgaacgact ttagcacggt aaacttcatc
     5461 cactttggct tcacgaacca aaccagcaat aatttctttg gcacggttaa tagcatcttg
     5521 gtcactagag tagatagaca catttccttc ttcgtctata tcaatcttaa cacctgtttc
     5581 agcgataatc ttgtcgatgg tttctccacc cttaccgatg acaatcttaa tcttgtccac
     5641 atcaatcttg atcgtatcaa ttttcggagc agttggagcc aattctggac gaacttctgg
     5701 aatggttgct tcaatgatat caaggatttc aaaacgcgct ttcttggctt gagcaagagc
     5761 ctccgtcaag atttctgcag taatcccttg aatcttgata tccatttgaa gggctgtaat
     5821 cccatcacga gtacctgcaa ccttgaagtc catatctcca aagtgatctt ccaaaccttg
     5881 gatatctgtc aatactgtgt agttatttcc atctgagata agtcccatag caataccagc
     5941 tactggcgcc ttgattggca caccaccagc cataagggca agagttcccg cacagataga
     6001 agcttgagat gaagaaccgt ttgattccaa aacttctgct actagacgga tagcgtatgg
     6061 gaattcttcc aagcttggca agacttgagc aagagcacgc tcaccaaggg caccgtgacc
     6121 gatttcacga cgacctggcg caccgtaacg acctgtttcc cctacagaat attgagggaa
     6181 gttatagtgg tgcataaagc gtttcttgta ctctggatcc aaaccatcaa tgatttgagt
     6241 ttctcccatc ggagccaagg tcaagactga aagagcttga gtttgcccac gagtaaagag
     6301 acctgaacca tgtacacgag gaaggaagtc aacaaccgca tccaaaggac ggatttcatc
     6361 gaccttacga ccatcaggac gcaccttgtc ttctgtaatt aaacgtcgca cttctgcgtg
     6421 ttccatttgt tccaagattt cagccacatc acgcataata cggtcaaatt cttcgtggtt
     6481 cgcatatttt tcttcgtaaa cggcagtcac ttggtctttc actgcttgag ttgcagcttc
     6541 acgggccaat ttctcttcta cttgaactgc cttttggagg tcactgttgt aggctgcaat
     6601 gatttcagct tgcaattcag catccacgtg aagcaattcc acttctgctt tttctttacc
     6661 gacagcagca acgatttctt cttggaaggc aatcaattct ttgacagctt cgtgcccttt
     6721 aagaagcgct tccaacatga tttcttctga caattctttg gcaccagact ctaccatgtt
     6781 gatagcgtgc ttggttccag ctactgtcaa ttcaagaaga gattgctctg cttgttcttg
     6841 acttgggttg atgatgattt ggccatctac atatcccact tgtaccccag caattggtcc
     6901 gtcaaatgga atatctgaaa tagacagtgc caaagatgaa ccaaacatag cagccattgg
     6961 tgcagatgca ttttcatcat aagaaagcac tgtattgatg acttggactt cattacggaa
     7021 accttccgca aacataggac gaatcggacg gtcaatcaaa cgcgctgtca aggtcgcatc
     7081 tgttgaagga cgtccttcac gtttcataaa gccaccagga aacttcccag ccgcatacat
     7141 tttttcttcg tagttgactt ggagtgggaa gaaatcccca gttgccattt tcttagacat
     7201 aacggcagca gtcaagacag ttgactcacc gtaacgtacg acaacagatc catttgcttg
     7261 cttagcaacc tgaccagtct ctacaattaa ctcacgaccc gcaaaagtcg tttgaaacac
     7321 ttgttttgcc attttaatcc cctttggatt gatgaaatta tacgccttgc ctacaaagat
     7381 caagatacca aggacgtcaa aagcaaagta aaaataggaa actgacgaag tcttcgatga
     7441 agacaagaca gtttatcttt tttacacagc ttttcggccg tgttcaatta cacaagatat
     7501 tttggacggt tcggcttgcc gaacatttct gtagaaaaat aggaaggtga cgtcgcactc
     7561 gacgagtgct aggaagctta tcttttttcc taagaaatga gaccaaaatt caagtcatca
     7621 agataccaag ccgtcaagca actcaaagga agataggaaa tcgaacgacg gagcgactac
     7681 tcctagggag atttatcttt ttccacagag ttgtaggcaa gttcagtttt caagatacat
     7741 cattagaaag gtttaatact aaagtatcta aagctttcac gctaatcgct atcgggcgat
     7801 tagttaaatg ctttactaac tctctcgtca aataacatcg atttgactca ctcgtgtcgt
     7861 taaatcttac agtttaaatg cattgtatta tttaatacct tcatctttgt atcaagtacg
     7921 tacagaattt attttatcat atttttctta aaaagtgagg tctttaccat taaaaaggaa
     7981 ccattcccct cacctgagaa gaatggtttg cttttattat cctagagact ggtgattaaa
     8041 caaggcatgg gttgcttgat ggatgtattt tgctgtatca gcattattca tcgtatagag
     8101 atgcacaccg gcaacatcct gagttaccaa gtccacgatt tggtccactg cataggcaag
     8161 tcctgctgct ctgagcgact cagggtcatg ctcatacttg tctaagatgg ctttaaattt
     8221 gcgtggaaga tggatattct cacaagtctt caagagtcgg agagcctgat ttcgattcag
     8281 aattggcata attcctgcat gaatgggaac atcaatccca gccaagatgc acttgtcctg
     8341 aaaatcatag aagcgctcat tgtcaaagaa gagctgagtt acgaggctcg aacagcctgc
     8401 attcactttc ttcttaagat tttgaatatc tgaaatctga tttggcgaat ctggatgccc
     8461 ttctggatag caagctccaa taatatcaaa gtgaggggtt tgttccttga taaactcaat
     8521 caagtctgtt gcatagcgga aatccttttg tggttccacg tctggaataa tatccccacg
     8581 aagagccaag attttctgca ccccaacttt gtccaagtca gcaatagttt cagcaacctt
     8641 gtccttagtt agataaatag ctggcaagtg ggcaatggtc ggaatcgcca aatcattttg
     8701 gataaagtca gccaaacgaa ccgtcgtttc cttgatatta aatttattat tgctggcagt
     8761 tacactgata aaatggggag ccaactcctg catatactgc aaggctgaaa taatgttacc
     8821 attacccacg gctgggtttg gagggaacac ttcaaatgag agtgacggtg tttggcgtga
     8881 catatgtaat aaccttttct agttgatttc tttttgaaca accactgtat ggagagaaat
     8941 ccaatcttac aatttctcac gcgcagcttt agctgcttca acaaggcgga tcaagctttc
     9001 ttttgtttct gggataccac gtgttttcaa accacagtca gggttgatcc aaactttctt
     9061 gcttggcact ttagcaagga tggcttcgat tgtgttgtcg atttcgcctt catttggtac
     9121 acgaggtgag tggatatcgt aaaccccagg tcccacttct gtttggaagt ttttcgcttt
     9181 gagttcgtcc aagatttcaa ggtttgaacg gctagcttca aaggaaataa cgtctgcatc
     9241 catgttatcg atagctggga tgatatctgt aaattctgag taacacatgt gagtgtggat
     9301 ttgtgtgtct ggcgctactg ttgagtgtac caagcggaag gcaggaatag cccagtcaag
     9361 gtagtcttcg taccagtcgc tacggcggag tggcaatttt tcacgaagag cagcctcgtc
     9421 gatttggatg attttcacac cagcagcttc aaggtcaagt acttcatcct tgatagcaag
     9481 ggcgatttgg agagttgaat ccttgataga gatgtcttca cgtgggaatg accagttaag
     9541 gatggtaaca ggtccagtca acataccttt aacaggtttg tttgtacgac tttgtgcata
     9601 gctagaccat ttaacagtga tagggttaag acgagtgaca tcaccccaga tgattggtgg
     9661 ttttacccca cgcataccgt atgattgtac ccatccattt ttagagaaga ggtatcctga
     9721 caagttttga ccgaagtact caaccatgtc attacgctca aattcaccgt gaacaaggac
     9781 atcaaagtca atatcttctt gccacttgat ccattcgtca atcgtttcag caaggaaagc
     9841 gtcgtactct ttttgagaca attcaccttt acggtaagcc aaacgtttgg cacgaacttc
     9901 ttttgtttga gggaatgaac caatcgttgt tgttggaaga gctggaagtt tgaaagcttc
     9961 ttcttggata gcttcacgtt ctgcaaaggc tggcaaacga gtgtagtctg cgtctgtcaa
    10021 gccagcgata cgcgcacgaa gttcagcatt tttaccaaca cgctcagtcg caaagagttc
    10081 tttgttggct gcaagagctt ctgaaccttg accattgcgg atagcatcca agtcacggat
    10141 ttcatccaat ttttcaactg caaaggcaaa gtggttcaag agtgctggtt caaattcttc
    10201 attagcagtt gtaaatggca catgaagaag tgagcaagag cttgtcaaaa cgatgttttc
    10261 agctgggatt tgctcaagaa cagccaagct cttttcgtag ttgttgcgcc agatgttttt
    10321 accattgaca atacctgcat agagagtctt gtcagctggg aagccacctt taacgagttc
    10381 aagagttttc ttaccttcaa caaagtcaag accgatagca tctactggca agtttacaag
    10441 gtcagcgtat acgtcacgaa catcaccgaa ataagtttga aacaagactt caagaccttt
    10501 tttgtcagcc aagagtttgt tgtaaaggtt caagaagaga gctttttctt cagctgtcaa
    10561 gtcttttaca agagccgctt catccaattg gatgcgagtc gcaccaaggt cagccaattt
    10621 agcaaaaact tcttggtaag cagccacaaa gctatctacg aagtcgtctg ctttcacgcc
    10681 ttcttcaaag tctgacaatt gaaggaaagt gaagggacct acaagaacag gacgagtgtt
    10741 caatccaagt tctttggctt cttgaaactc atcaaaaatc ttgtgacctg caagtttgac
    10801 ttgagtgtct ttttcaaatt taggaacgat gtagtggtag ttagtgttga accatttctt
    10861 cattggaagg gcgcgaacgt cccctttttc tccctggtaa ccacgtccca aagcgaagta
    10921 gcgctcaagg tcagacaagt ccaagttttg aactgaggca ggcactacgt tgaaaaggaa
    10981 agccgcatct aggaagttgt catagtgaga aaagtcattt gatgggattt cagtgatgcc
    11041 tttttctttg acgatgttcc agtgtttagc acgcaagtct tttgcggctg caaggagttc
    11101 ttcttctgag atttcttttc taaagtattt ttcagttgta aattttaatt cacggaattc
    11161 gcccaaacga gggaaaccga tgattgtagt tgacatgatg tgtcctccaa aatttgttgt
    11221 tgaaactatc ttaacagaaa agaaagcgtc tgtataattg taaaaaatta ggctttgata
    11281 tagtttgaaa ctatatatct gtttcggaca aaagaaaaag acttgaagca aacgtctcaa
    11341 atcctttgta attcttactt tacagctata ttccaatta
//
LOCUS       DAVLSL010000057        11310 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLSL010000057 DAVLSL010000000
VERSION     DAVLSL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.058"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(286..462)
                     /locus_tag="VXN30_001168"
     CDS             complement(286..462)
                     /locus_tag="VXN30_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357796.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            609..1031
                     /locus_tag="VXN30_001169"
     CDS             609..1031
                     /locus_tag="VXN30_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1357797.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            complement(1293..1685)
                     /gene="rpsI"
                     /locus_tag="VXN30_001170"
     CDS             complement(1293..1685)
                     /gene="rpsI"
                     /locus_tag="VXN30_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1357798.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(1705..2151)
                     /gene="rplM"
                     /locus_tag="VXN30_001171"
     CDS             complement(1705..2151)
                     /gene="rplM"
                     /locus_tag="VXN30_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1357799.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            2508..2825
                     /locus_tag="VXN30_001172"
     CDS             2508..2825
                     /locus_tag="VXN30_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1357800.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(2870..3682)
                     /gene="folK"
                     /locus_tag="VXN30_001173"
     CDS             complement(2870..3682)
                     /gene="folK"
                     /locus_tag="VXN30_001173"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1357801.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(3725..4279)
                     /gene="folE"
                     /locus_tag="VXN30_001174"
     CDS             complement(3725..4279)
                     /gene="folE"
                     /locus_tag="VXN30_001174"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1357802.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(4260..5582)
                     /locus_tag="VXN30_001175"
     CDS             complement(4260..5582)
                     /locus_tag="VXN30_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1357803.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(5584..6558)
                     /gene="folP"
                     /locus_tag="VXN30_001176"
     CDS             complement(5584..6558)
                     /gene="folP"
                     /locus_tag="VXN30_001176"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1357804.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(6653..7360)
                     /locus_tag="VXN30_001177"
     CDS             complement(6653..7360)
                     /locus_tag="VXN30_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1357805.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGLVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(7413..8831)
                     /locus_tag="VXN30_001178"
     CDS             complement(7413..8831)
                     /locus_tag="VXN30_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1357806.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIYWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            9021..9833
                     /locus_tag="VXN30_001179"
     CDS             9021..9833
                     /locus_tag="VXN30_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1357807.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            10172..11191
                     /gene="adhP"
                     /locus_tag="VXN30_001180"
     CDS             10172..11191
                     /gene="adhP"
                     /locus_tag="VXN30_001180"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1357808.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKGKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3404 a         2529 c         2127 g         3250 t
ORIGIN      
        1 tgaaaaaatg tagtggacaa aatatctttt aacaaaccaa gagtttatta aagagttatc
       61 acttttcaac ttttctaagc ttatgcagtt gtgaaacaaa ctacttttaa actattaact
      121 aagataggat tgataaataa tttcaaactc ttactagcaa tcatacgata ttcaagctca
      181 cgtgcttttt tccttcctgc ttatttctta gaactgaaga acccggatcg gtatataaat
      241 tatccggatc aacatagtca taagactcat aacagttgcg cttcattaag tcatccccag
      301 agcaagagct tcatctcgta atttttcaac atcactaacc gtaggtcgcc atccttcaat
      361 catatttgta cttaaagcat accaaacact cttaaaaacg gatcggtttt caaaagctat
      421 tcccatgatt gtcatctttt ctttatctat atctaaggac atatgctacc tcctttagat
      481 acattatacc atgtttctct gtagctttta aaaattttat tttgtttgtc atatctaagt
      541 tttcagtacg cttatcctat tttataagcc tcaaacccaa atataaaacg cattaaccat
      601 ctgataatat gcgttttatt gtggaaacat ttcccatttt ctctatttct aatgacttac
      661 ttcaacttct gacttcatgg aaaaaacaac aaaaatatga actggcaaag tttggtatca
      721 tcagtaatcc agaacaattc atctttactt acatagatac caaaggcaat atcaataaac
      781 ctttacattc tgactatctc aataataaga tgaaaactat cagaaaacga cacccagagc
      841 ttacacacgc tacgccacac aaactacgtc ataccggagc aacgctcgct aaacaagcag
      901 gaatgagctt agaagccatt tctgaagctc taatacatag cgacacaggt acaacacaga
      961 tttatgtcaa tacttctaat gtagtcccta tggccgtcgg tgaatttgcc ttacagtctc
     1021 taaaacaata aggtgaaaat aaggtaaact ttgaggtgaa ctttttcaaa aaacataaaa
     1081 aaagcaccca tgaggtaaac tcttgagtgc tttgaaacgt tgatataatt gtattgatta
     1141 acgttttgag gcaactgacc ttgtcaggtt gcagccacat ttgtaagcga ctaaagtcgc
     1201 aacaactgtg tcaattgcac caatttagta agaaagtata aaaaaagaac accccgaaag
     1261 gtgctctgta taagtatagt aattctttcg aattaacgtt tactaaattg tgatgcttta
     1321 cgagctttct taagacctgg tttcttacgt tcaactttac gtgagtcacg tgtaagaagt
     1381 cctgcgcgtt tcaatgaatc gcggaagtct gggtctactt gaagaagggc acgagcgata
     1441 ccgtgacgga tagctcctga ttgaccagcg tatccaccac ctacaacgtt aacgaaaacg
     1501 tcgtatgaac ctacagttga agtaactgcg aatggttggt tgatgacaag acgaaggtca
     1561 gcgtgtggga tgtactcttc aacatctttt ttgttaacag tgattttacc agttcctgga
     1621 acaaggcgaa cgcgtgcaac agcgttttta cgacgtccag tacctgcata ttgtgcttgt
     1681 gacatacttt atttgttcct ttccttagat aagtcctgaa atgtcaagaa cttctggttg
     1741 ttgtgcagca tgagtgtgct cagctccaac aaatactttc aacttcatac cttgagcgcg
     1801 tccaagtgta ttgtgtggaa gcataccttt aactgatttc tcaatcaaac gtactgcatt
     1861 tttagaacga agttcacctg cagagatttg tttcaatcca cctgggtggt ttgagtgagt
     1921 gtagtagatt ttatcagttg cttttttacc agtcaattta actttttcag cattgataac
     1981 aatcacgaag tcacctgtat cagtgtgtgg tgtaaatgtt ggtttgtttt ttccgcgaag
     2041 tacgctagca actactgcag aaagacgtcc aagtggtaca tcagttgcgt caactacgta
     2101 ccatttacgt tcaacttggc ctggtttagc cataaatgtt gttttgttca tgatttctcc
     2161 tatatatagt tcgatttttg tttacggtga tgggtgttcc ttcccatcga aaagtatttg
     2221 gaaggttccg gggcctttca aatggggtaa acaataccgc ctactatcat accaaatttc
     2281 acccctaaaa gtcaatagat atgaaaaata tttttcggta acttctcaaa gtaactgaaa
     2341 gttatataat caagttaatt tgggaattta cctattaact tcggtagatt tttgggaatt
     2401 tttttccttc tttttccctg tagttaaaat ccttttcaaa aaatcacctt ttcttgattt
     2461 ttaatcctat ttgctgtatg ataagggaaa agaaaggaga cagagatatg gcttttacca
     2521 atacccacat gcggtctgct agttttggca ttgttaccag cttgcctgat gacatcattg
     2581 actctttttg gtatattatc gaccatttct taaaaaatgt ctttgaattg gaagaagaac
     2641 tcgagtttca attgcttaat aaccaaggaa agattacctt ccacttttca agtcaacacc
     2701 tccctacagc cattgatttt gactttaacc atcctttcga ccctcgttat cccccaagag
     2761 tactggtttt agacatggac ggtagagaaa ctatcctcct cccagaagaa aatgacctat
     2821 tttaaaaact ctagccttca gttgcaagta actgaaaact agagtttttc tattttttca
     2881 aagcatcata caagttacgg atcggttgtt ttaatatcgg atggataaaa tgaggcgcaa
     2941 tttcctgtaa ggactcaagg acaaaaaggc gttccgctat gtaaggatga ggcaatatga
     3001 ggtcgtctgt ataaaggatc tggtcctcca caaagagcaa gtccaaatca atcaaacgag
     3061 gtccccaatg cacttctctc acccgtccca gctctgactc aatggctaac aaggtttcta
     3121 acaagtcttg tgctggtagc caggtttcca cctcaaccac ttgattggca aagctatcct
     3181 gctccactcc accccaaggc tccgtcgcta agacactgga ctctttgaga atatagatgc
     3241 cacgagctcg cagtttgtca atggcttgct tcaagtttgc ttgtttatct cccatattgc
     3301 ttcctagggc gataaaggct cgttgcttgc ggcgatgaat ggttaccgag caagtatcta
     3361 gtgacaaatg caccggtgcc caaggttttt tcagttccaa cttcatttct tggacaagag
     3421 gataagactc aaaggtacgt tccaccagtt tataggctac cgtttcaatc aaatcttcac
     3481 tcgtttcctg aaaccaagtc gtccactgct gacacaattc tccgtaatgg acagaggctg
     3541 ttaaatccaa gtctgtagct gccttggtca tatcatagga taggatggct gaaacgacaa
     3601 atttctgccc caattctttc tcactaggaa aaagaccatg ataggcaaac atttccaaat
     3661 ccttaatctg cagttgatcc ataatgattc ctttctagaa aaatccgctt gaaacggatt
     3721 ctttttatag ccccattaaa cgataagctt ggtcgcggag gtccttatct gtttcaaata
     3781 gaccacgagc tactgtcgtc aaggttgcag tgcctggttt tctaacacca cgcatactca
     3841 tacacatatg ttccgcctca atgacaacaa aggctccttt agcacctaga tagtccatca
     3901 aggcatcggc cacttcgata ttcaaacgtt cttgaatttg tggttttttc gaataaactt
     3961 caaccgtacg ggctagctta gacaagcctg ccacacgacc atctggaatg taggcaatgt
     4021 gcgctctacc ataaaatggc aagaagtggt gttcacacat ggtatggaaa aagatatcct
     4081 tttctaccac catattatcg tcaataattt caaaggattt tgacaaatgt tcctctgctg
     4141 tttgaccaag acctgaaaaa atctcttgat acatacgggc tacacgagca ggtgtttcct
     4201 gcaagccctc gcgattagcg ttctctccta cagcctcgat aatcattttt acagctgctt
     4261 caatcttttg tgtatccatt ctcgttcttc ctctccatca gataggccct cacttggctc
     4321 aagaaataca aggaacctgt gacaatccta actgtttgtt tctcttcttt tttatctgtc
     4381 aaattctgct ctagaaaatc atgccaatct tggtagctga gatttctaga cttagctgcc
     4441 tctttcagca cgctttcatc cgtcgcccga ctatccgcaa aatgtgttag agtaagctcg
     4501 gtatctggca tagctcccag caagtccaac atatccccca aggctttggt tttgatacaa
     4561 gtgaagagga tttccttatg ataatccgca aaacgttctt gcaaggttac caacaaggcc
     4621 ttgatagcat ggggattatg ggctccatcc aaaatcatca agggatctct tgacacgatt
     4681 tccaaacgcc ctggccaact tgtttcttcc aaggcttgac caagaaagtc attgcttgct
     4741 agctctcgac catcttcttg acaaaaagta tcaagtaaag ctatggccat cccagcattc
     4801 tctatttggt acaaaccaag caggctagtc tggaagcgac cttgtctgac agcacttgta
     4861 tagtcaaaga cttcccctgt caccacactt tcttgatgac gaacctgata atctgtcccg
     4921 taggcaagtc tcggcgcatc tttcccttcc gcaatgcggt caatcacagt caaggcttct
     4981 ggagcaatac gccctgttac caagggcatc ccttgtttga taatacctgc cttctgctct
     5041 gctatggcct ccaaggtgtc accaagtagg gccacatgat ccaagccaat agttgtaatt
     5101 cctgtcaaaa tgggctgaca gacattggta ctatccaaaa gtccacccat gccaacttcc
     5161 atgatagcca catctacttg ctctgaggca aagtagtcat aggctagggc tgtgataatc
     5221 tcaaactcgg ttgtgccctg taaattggcg accgcttctc cctccagcaa agactgatag
     5281 tctgccatga gagcttctag cctcgcttct gagatcgatt ccccattgat gctaatctgg
     5341 tctgtgtaat gaatgagata gggcgaacta aacacgccaa ctctcagccc tagcttttct
     5401 agcatctttt tcaaaaaagc aatagtcgag cccttgccgt tagtccctcc gatatggaga
     5461 accttgagtt taagatgggg attgccacgc aaagctaaca gttccaccat tcgttccaag
     5521 ccaaaatgcg gttgatccgt ccggtagtta gcaatccact gattgttttc aatttctttc
     5581 atcttattta tattgtttta aatctaaatt ttccgcttca tcagccagac gaatggcaga
     5641 ggcaatttca actgccatcc tgtgactagc tacgtcatgc acgcgcacca cttctacacc
     5701 ctgtctcgca gcgatactag ttacatgagc cgaagccgtg tcccgatttc ggaaaccaag
     5761 ctctgtctca ggattgactt caaacccact ttcttcaagg atattgatga caaatcgctt
     5821 gcgcgacact ccgagaaaga ttggatagcc cttctgatgt agtttatcca ggtcccgtaa
     5881 aaggagaaga ttttctttct tggtcagacc aaagccaatt cctggatcca acaggatatt
     5941 ttctggtgca ataccagctt ctgccgctct cgctagtgct cgttcaaaga aagccaccat
     6001 caagtcttcg attggcaatg tttcaaagtc agctaactct ttttctgtaa aggtttgacc
     6061 aaagccaaaa tgagggaaga taagcgaact aggatgctga ggtcgagcca taactgggtt
     6121 aaacatgatg accactttcg ctccagcttt agcaaccaca tgggccattt tttcatcacc
     6181 catgagacca gtgatatcat tgactagatc ggcaccagca gccaaagcag cctctgctac
     6241 ttgactcttc caagtatcaa tagagatgag gacatcactt tccttgcgaa tcgctttgat
     6301 cactggaaca acacgctgga tttcctcttc tatctcaaca tagctacttc ccggccgagt
     6361 cgattctccg ccgatatcga gcatactggc tccttctgct atcaatttac gagcctgctg
     6421 gagtgcctgc tcaagagcaa aaaattgacc accgtccgaa aaggagtctg gggttacatt
     6481 gataattccg caaataactg tctttgcatg attggcttta cttgacatat cggtcactcc
     6541 ctcaaggatt ttcatcatat tatttttcta ttttaccata aaaagaaaaa gatggacacg
     6601 attgcattca tctttcccta gtagaaagag gtcggttcct acataaaaac ttttaaacag
     6661 aaattcctaa tgtccgactc ataatcacca caagagccaa caaacagaaa gcaatcccat
     6721 taacaatcat gtgaagcaag atcgacattt ccaaacgttg ggtcttgtag gccgtccaag
     6781 atagaactgt cgacatacct ccataaatca ataaagaagg taaattactt ggttgatgca
     6841 ataaagcaaa cacaatcgta ccgactacaa atcccaagtt ctccttgcct cggaaaatct
     6901 ttttaggaac aagcccacgg cacaagattt cctcacaaat tggagcaagc aaggctagca
     6961 agaagaagct agaaatcaga gaactattct gaaccatatc gttaatctga gactggttag
     7021 ctgttgtcgt ctcatttgac agttgcaaca agatagaacc aagtatattt gaacagataa
     7081 tgactaaata actcaagccc aaacgtgcca aatccttagt tctaaaaaaa gaaaaattaa
     7141 aacttgccaa ttgcgtttta cgagctccaa taataaatag caccagaacc acaatagaaa
     7201 taccagtaac tatcagccct gactgtagca gggctacttc ttttaaagtc aaaatagagg
     7261 taaccactag gggaacctga tataaaagga cggcaagtaa aaagaccaaa atccagcctg
     7321 cacgattcaa caattctttc cacatatttt tctctttcat cacctatctc ctttgtatta
     7381 aaaaaggggg aaaatccccc tgggtcattc tattataagg ccatgctgat gtagttaagg
     7441 ataaacaagg catccaaaat ccaaatcatg gcatgaacat ctttaacttg acctttgaca
     7501 agcttagtca aagtgtaagt caagaaacca actgcaatcc cttgagtgat agagtagctg
     7561 aatcccataa agatagatgt gaagaaggca ggaactgctt cagacatatc atcccaatag
     7621 atatttttca agctaccaag catcataatc ccaacgataa tcaagattgg agctgtagcc
     7681 gctgttggta cgatcgctag aagtgggcta aagaagcttg aaatcgcaaa acagatagct
     7741 acaaccaagg ctgtcaaacc agtacgtcca cctgcaccga taccagcagc agactcaaca
     7801 taagtcgtta cgtttgaagt acctgcaatg gcaccgactg tcgttccaat caaatcagag
     7861 taaagagcct tatccaattt ggctgattgg tgattttcac catttgtcgc tacaatacca
     7921 actttttcac ctgtaccgat caaggtacca attgtgtcaa aaatatctgt caatgagaag
     7981 gcaagaatgg ccatcagagt ttcaggcaag cgagctgtat ctgaaaccaa agctcccaaa
     8041 ccttctgaac caagagctgc accaaagatt gtcttcaaat cttcaaaagc tgcaccaaca
     8101 tggttattag caaaatcgat actagacaaa tcaaccaaac caactgcaat agcaagaaca
     8161 gttgttgtca agatagagag aataattccc cctttaatcc ctttgatgac aaagaagata
     8221 gtaatggcaa gtcctgcaag agccactaaa acagctggat tattaaagct gaccaatcct
     8281 ggaactgctg aagagtttgc tgcaatcgtt gcttgagctt tgtcagcccc ttctcctaca
     8341 acagtatagt tgcctggatc aatcgtgaat ttcaaaagtc cagcattctt aatccctaca
     8401 taggcaagga agacaccgat accagctgaa atagctgagc gaagagcatt gggaatcgat
     8461 tcaatgatca ttttacgaac atttgtcaag gtaataatca atgaaataat cccacagatg
     8521 aagaccatag ctagggcttc ttgccaagaa taaccaagcc cgaatacaac tgtaaaggta
     8581 aagaaggcat tgagtcccat acctggcgct tgggcataag gtaagttagc ataaaaagcc
     8641 atcatcaagg tacccgctac tgcaccaata atcgtcgcta ggaagacgcc ctgagcaggc
     8701 attcctgttt gtgaaagtat ttgtgggttt acaaagagaa tatagctcat tgcaaagaaa
     8761 gttgttaaac cagcgagaac ctctgtacga acgtctgtac cgttctcttt tagtttaaat
     8821 aatttgtcca tcatcaatct ccttttaatt tttacgaaca ataatagaat tatatcattt
     8881 atcattcact ttttcaatgt atttgtttga tttgtttaag aaattaatcc aatttcattt
     8941 tttgtttgga atctgctttt ctgcctgctt tctgttataa tagaagacat cttatctgaa
     9001 aacacactag aaaggtccat atgacgaaaa aaattattgc agttgacctg gatggaaccc
     9061 tgctcaactc agacagtcaa atttctgact ttaccaaaag aaccatcaaa aaagttgctg
     9121 aaaaaggcca tcaggttatt attacgacag gtcgccctta ccgtatgtca aaagattttt
     9181 accgtgaact gggcttagac actcctatga ttaacttcaa cggatccctt actcatttac
     9241 cagaccaagt ttgggatttt gaaaagtgtt tgactgtaga caaaaaatat ctgctagata
     9301 tggttcaacg ttcagaggac attcaagccg attttatcgc tggagaatat cgtaaaaaat
     9361 tctacattac aaatcccaat gaagaaattg ccaatcccaa actatttggt gtagaagctt
     9421 tccagcctga agatcaattc cagcctgaat tggtgaccaa ggaccctaac tgtatcctct
     9481 tgcagaccag agccagtgac aaatatgcct tggcaaaaga catgaacgcc ttctaccagc
     9541 atcaactttc tatcaacacc tggggaggtc cgctcaatat ccttgaatgt accccaaaag
     9601 gtgtcaacaa ggcctttgct ttggactact tgctcaagat aatgaatcgt gacaaaaaag
     9661 atttgattgc ctttggagat gaacacaatg ataccgaaat gctcgctttt gctgggaagg
     9721 gttatgccat gaaaaatgcc aatccagagc tactccctta tgcagatgag caaatttccc
     9781 ttaccaacga ccaagatggg gttgccaaaa ccctacaaga cttattctta taacctatac
     9841 tgatactcaa tgagaatcaa agagcaaact aggaagctag ccgtaggttg ctcaaagcac
     9901 tgctttgagg ttgtagatga agctgacgtg gtttgaagag attttcgaag agtatgacca
     9961 gcttgcaagc tggtctttgt gctcttttca cagtctcatc aaccagttaa tcgattgttc
    10021 tactttttgt ctccaaaacc ttggaaaagg ctttcttttg tgatatactt cacatataaa
    10081 tacaagagag ctcgtcacac tcgactctgt tgacaaaaaa tcaatccagt cccgttgtcc
    10141 ctgttctcgg tcaatatcaa ggaggatagc tatgaaagct gttgttgtaa atccagaaag
    10201 cactggtgtt gctattgaag aaaaagtact ccgtccactt gaaactgggg aagcacttgt
    10261 agaagttgaa tactgtggcg tttgccacac cgatctccac gttgctcatg gtgactttgg
    10321 tcaagtccca ggacgtgttc ttgggcacga aggtatcggt atcgttaaag agattgctcc
    10381 agatgtgaaa agccttaaag tcggtgaccg cgtcagcgtt gcttggttct ttgaaggatg
    10441 tggcacttgc gaatactgta caactggtcg cgaaaccctt tgccgtacag tgaaaaatgc
    10501 tggctactca gtagacggtg gtatggctga acaatgtatc gtaactgctg actatgctgt
    10561 caaggttcct gacggacttg atccagccca agcttcttct atcacatgtg ctggagtaac
    10621 aacctataaa gctatcaaag aagcaaaagt tgaaccaggc caatgggttg ttctttacgg
    10681 tgctggtgga cttggtaacc tcgctgttca atacgctaaa aaagtattca atgctcatgt
    10741 tatcgcagtc gatatcaaca atgacaaact tgcccttgca aaagaagtag gcgctgacat
    10801 tgtgattaac ggcctcgaag ttgaagatgt agctggactc attaaaggaa aaactgatgg
    10861 aggagctcat tcagctgtcg taactgctgt gtctaaagtt gccttcaacc aggctgttga
    10921 ctccgttcgt gctggtggtc gcgtcgtcgc tgttggtctt ccttctgaaa tgatggaact
    10981 cagtatcgtt aaaacagtcc tcgatggaat ccaagtcatc ggttctcttg tcggaactcg
    11041 taaagactta gaagaagcct tccaatttgg tgcagaaggt ctggtagccc cagttgttca
    11101 aaaacgtcca gtagaagatg ctgttgccat tttcgacgaa atggaaaaag gtcaaatcca
    11161 aggacgtatg gtactcgact tcacccacta atctaataga aacctatttt aaaagttgga
    11221 agcttcttct aactttttta tattaaattt ttaaataaga attcaagatt actccttaaa
    11281 aactcaacat atcaacatat aaaaataaaa
//
LOCUS       DAVLSL010000058        11274 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLSL010000058 DAVLSL010000000
VERSION     DAVLSL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.059"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            33..1559
                     /locus_tag="VXN30_001181"
     CDS             33..1559
                     /locus_tag="VXN30_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1357809.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            1646..2565
                     /locus_tag="VXN30_001182"
                     /pseudo
     CDS             1646..2565
                     /locus_tag="VXN30_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            2700..3659
                     /locus_tag="VXN30_001183"
     CDS             2700..3659
                     /locus_tag="VXN30_001183"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1357810.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            3756..4595
                     /locus_tag="VXN30_001184"
     CDS             3756..4595
                     /locus_tag="VXN30_001184"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1357811.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(4643..4813)
                     /locus_tag="VXN30_001185"
     CDS             complement(4643..4813)
                     /locus_tag="VXN30_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1357812.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            4934..5818
                     /gene="miaA"
                     /locus_tag="VXN30_001186"
     CDS             4934..5818
                     /gene="miaA"
                     /locus_tag="VXN30_001186"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1357813.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            5811..7049
                     /gene="hflX"
                     /locus_tag="VXN30_001187"
     CDS             5811..7049
                     /gene="hflX"
                     /locus_tag="VXN30_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1357814.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            7042..7665
                     /locus_tag="VXN30_001188"
     CDS             7042..7665
                     /locus_tag="VXN30_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1357815.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            7680..8609
                     /locus_tag="VXN30_001189"
     CDS             7680..8609
                     /locus_tag="VXN30_001189"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1357816.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            8630..9385
                     /locus_tag="VXN30_001190"
     CDS             8630..9385
                     /locus_tag="VXN30_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1357817.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(9439..10347)
                     /locus_tag="VXN30_001191"
     CDS             complement(9439..10347)
                     /locus_tag="VXN30_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1357818.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            10411..10680
                     /locus_tag="VXN30_001192"
     CDS             10411..10680
                     /locus_tag="VXN30_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1357819.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            10680..11060
                     /locus_tag="VXN30_001193"
     CDS             10680..11060
                     /locus_tag="VXN30_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1357820.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
BASE COUNT         3431 a         2077 c         2514 g         3252 t
ORIGIN      
        1 cgtaaacaat agaaatttaa aggagatttt tgatgaaatc aaaaatagtt ttgggagctt
       61 cgctagcgat cgcaacactt agcttagtat ctcttgtcga gattgaagga ctctcaccgt
      121 ttttaataga gaatgttagc gcaaatactc attctgcaaa taaagttatt aatcataaag
      181 taagtattta cttagaaaat gctgatgaag gtaaaggtct aactgtaaac ttttcaacag
      241 acagtgttag tccaaattta tttgatgagt tcgaaaaaaa gagtggaatt acaataacta
      301 ctatgttagt gaatgctaag acaggtgaag ttgtagaaaa aagattaaca ccaagcgttt
      361 ttttacgttc caatgattta accagtggga ccatatcttc gtttattttt agtgaatatc
      421 ctgatggaga atataaatat gttgtgtcta aaggagactt tatcgatcct aagactcagt
      481 ttaaacatca gtatagagga gaatctccag tttttagaat acgtaatcga aaatatgtgg
      541 agttgggaac aactgataaa aaattagatg aaagacgtga taatagcgtt tataaagatg
      601 gggttgttga acacaaggta aatttaagtt taacaagtta tcaaggaggt aatggagtta
      661 ctgcaatttt ttcaactgat agtgttaact ctaacttact taattctttt ggtgaaaagg
      721 ctaaaaaagt tttgattagg tctaagttaa ttaatgttaa gactggcgag gttattgatg
      781 aaacgttttc tccaaaagta agtttaacct ctaaaatttt gaaaagtggt tctacagcag
      841 tattttattt tatagattta actgatggcg aatataaata tgttgcgtat gaaagccagc
      901 aatatactga tcctcaaaca acattgactc atcagtatag aggagaatct cctattttta
      961 gtatcaaaga tggaaaattt tctggtttag tatctgcctc aaaaccagac gaaaacccga
     1021 agccaacgcc aaaaccggat gagaaaccaa aaccttcagc accacaacag gagaaaacaa
     1081 aacctacagt tcagtctggt tgggtaggtt catcttatta tcaaaatggt aaaaaagtca
     1141 ctagtaagtg gatttttgat aaaaaatata attcatactt ctatctagat gcatcaggaa
     1201 attatgttca aaacgcttgg gtaggtaact attatctcaa atcaggcgga tacatggcta
     1261 aaagtgagtg gatttatgat aagaactatg gttcatacta ctatctaaca tcagaaggca
     1321 gctacgctcg taacacttgg tcaggcaatt attatttgaa gtcaaatggt aagatggcta
     1381 aaagtgagtg ggtatacgat agtaactaca aatcatatta ctacttgaca tcagaaggaa
     1441 gttatgctcg taacacttgg gtaggcaatt actatcttaa atcaaacggt aaaatggctg
     1501 ttaatgaacg tacaccagat ggttatcgtg tagatggttc aggtaaatgg gttaaataag
     1561 aaactaaaaa ataggataat aggtaatatc agatgaataa aaaaaattat tttcatgtgc
     1621 tttagcattt tcaacgattg gaacattgtc aacgattgat aaatcaggag ttcttccttt
     1681 ttcagcgcaa acggtgcagg ctgacataca aacgcttacc ccaatggcaa aatattatat
     1741 tattcttccg aaggatgcag aaatatataa aacttggaga ggtactgtca atattcccat
     1801 tattgatgct acaaaaacta ctccagaatt atcttatttc aaagaagatc atcgcaacta
     1861 tatagctaat gagaataagt ctggtgccaa ttacatagag tggaagggaa cagtagaaga
     1921 gtttaaagag gccattaaga agttgactga taaaaaatca actactgcaa caccaaaaaa
     1981 ggatgaaaag ccaacaccaa aaccggatga gaagccaaaa ccaacaccta cagttcagtc
     2041 tggttgggta ggttcatcct attatcaaga tggtaaaaaa gtcattagta agtggatttt
     2101 tgataaaaaa tataattcat acttctatct agatgcatca ggaaattatg ttcaaaacgc
     2161 ttgggtaggt aactattatc ttaaatcagg cggatacatg gctaaaggtg aatgggttta
     2221 tgatgccacc tatcaagctt ggtattattt gacatcagat ggttcttatg cttacagtac
     2281 atggcaagga aattactatc taaaatcgga tggtaaaatg gctgtcaatg aatgggttga
     2341 tggtggacgt tattatgttg gcgctgacgg agtttggaag gaaggtcaag caagtacagc
     2401 ttcttctagt aatgatagca atagtgaata ttctgctgct ttaggaaagg caaaaagtta
     2461 taattcgtta ttccacatgt caaaaaaaaa cgtatgtata gacaattaac ttctgatttt
     2521 gataaatttt caaatgatgc agctcaatat gccattgatc attagatgat taacagatac
     2581 tggtaggcga aaaaattcct tataattcat ttacgcaaac ctttgcataa gtgaatggat
     2641 tttgatatac tagtactgta agcattttca attactacac aatgaaaaag gagaatatga
     2701 tgagtcaaaa gattattggg attgaccttg gtggaacttc tatcaaattt gcaatcttaa
     2761 caacagcagg agaaatccaa ggaaaatggt caatcaagac caacattttg gatgagggaa
     2821 gtcatatcgt tgatgatatg attgagtcta ttcaacatcg tttggacttg cttggattgg
     2881 cagcagcgga cttccaaggc attggaatgg gatcaccagg tgtggttgac cgtgacaaag
     2941 ggactgttat cggtgcctac aacttgaact ggaaaaccct tcaaccaatc aaacaaaaga
     3001 ttgaaaaagc tttgggcatt ccatttttca tcgataatga tgccaacgta gcagctcttg
     3061 gtgagcgctg gatgggtgct ggagataacc aaccagacgt tgtctttatg acactcggta
     3121 ctggtgttgg tggcggtatc gtcgcagaag gcaaattgct tcacggtgtt gctggtgcag
     3181 caggtgagct tggtcacatc actgttgact ttgaccagcc aatctcatgt acttgcggta
     3241 agaaaggctg ccttgagaca gttgcttcag caacagggat tgtcaacttg actcgtcgct
     3301 atgccgatga atacgaaggc gatgcagcct tgaaacgctt gattgataat ggagaagaag
     3361 taactgctaa gactgtcttt gatctcgcaa aagaaggaga cgaccttgct ttgattgttt
     3421 accgtaactt ctcacgttac ttgggaatcg cttgtgctaa cattggttca atcctaaacc
     3481 catcaacaat cgtcatcggt ggtggtgtgt cagctgcggg agaattcctt ctacaaggtg
     3541 ttcaaaaagt ttacgatgaa aatagtttcc cacaagtacg cacatctact aaattagctc
     3601 ttgcaactct aggaaatgac gctggagtta tcggagcagc atcacttgta ttgcaataag
     3661 ataatgagag aggaaaatgc taacctagag ttagtaatgt tcctctttta ttttttatgc
     3721 tatactagtt aggacaatga atgaggtgag agtaaatgac aaaagcagat acgattttta
     3781 aagagaatat tgaatgcatt ctaaaggaag gtgttttctc agagcaagct cgtcctaagt
     3841 acaaggatgg gactgttgct aattccaagt atgtaactgg tgccttttcc gagtacgact
     3901 tatctaaagg agaatttcct atcacaacct tgcgtcccat tgcaatcaaa tccgccatca
     3961 aagaagttct ctggatttac caagaccagt caaatagctt agaagtgctt aatgacaagt
     4021 acaatgttca ctactggaat gactgggaag ttggagacac gggaaccatt ggtgagcgct
     4081 atggtgccgt tgttaagaaa cacgacatta tcaataagct tctcaaacag ttggaaacca
     4141 atccttggaa tcgccgcaat attatttcgc tctgggatta ccaagctttc gaagaaacag
     4201 atgggctgct cccgtgcgcc tttcagacca tgtttgatgt ccggcgtgtt gatggggaaa
     4261 tctatctgga tgcgaccttg acccagcgct ccaatgatat gctggtggcc caccacatca
     4321 acgctatgca gtatgtggct ttacagatga tgattgccaa acattttggt tggaaggttg
     4381 ggaagttctt ctatttcatc aacaacctcc atatctatga taatcaattt gaacaagctc
     4441 aggaattgct ccgtcgggag ccgtcaaact gccaaccacg cttggtttta aatgttcctg
     4501 atgggactaa tttctttgat atcaaagcag aagattttga gttggtggat tatgaccctg
     4561 ttaagccaca gttgaagttt gacctagcta tttaaaagaa tagaaaaaag aagttgagaa
     4621 taatcccaac ttcttttgtt tcttaacgtg atacgcggcg acgagctgct tttttacgat
     4681 tttcttcgat gaaagctgct ttttgctctt ctggttcgat tactttcttt ttaaatgcgt
     4741 atactgcacc tgcaacggca gcgacagttc ctgcgacacc tgttacaaga cctttagcga
     4801 atcctttagc catgagtctt cctcctttat attctcaatc agccagcctc ctcaagaggt
     4861 cacatttttc tgactgacct ttttgtgtta taataatagt aacgaaaaaa tgggaatttt
     4921 tcaaggaaaa aagatgaaaa caaaaataat tgtgattgtt ggaccgactg ctgttggaaa
     4981 gacggctcta gctattgaag ttgcaaagcg ctttaatggc gaagtggtta gtggagatag
     5041 ccagcaagtc tatcgaggac ttgatattgg gacggccaag gctagtccag aagagcaggc
     5101 agctgttcct catcatttaa tcgatgttag agagataacc gagtcttact cggcttttga
     5161 ttttgtttca gaagctaaga tgactattga ggatattcag agccgtggca agctagccat
     5221 tatcgccggt gggactggac tttatatcca gagcttgcta gaaggttacc acctaggtgg
     5281 ggagactcct catgaggaga ttttagctta tcgagctagt ttggagccat attcagatga
     5341 ggaattagcc catttggtgg agcaagcagg ccttgagatt ccccagttta atcgtcgtcg
     5401 tgctatgcgt gccttagaaa ttgcccattt tggtcaggat ttggaaaatc aagagatctt
     5461 gtatgaaccg ctgattatct gcttggatga tgaacgtagt caactttatg agcgtatcaa
     5521 ccaccgagtg gatttgatgt ttgaggctgg gcttttggat gaggccaagt ggctgtttga
     5581 ccattcccct aatgtacagg ctgctaaagg aattggctat aaggaactct ttccttattt
     5641 ccgtggagag cagacctttg aggaagctcg tgagagtctt aaacaggcga cccgtcgttt
     5701 tgccaagcgt cagctgacct ggttccgtaa tcgcatgcag gtcacctttt atcagattgg
     5761 agaatctggt gtacaagacc gcattttaag ccagatagag gagtttttag atgattgaaa
     5821 cggagaaaaa agaggagcga gtcctgctga ttggtgtgga attgcagggt atggacagtt
     5881 ttgacctctc catggaagaa ttggctagtt tagcgaaaac ggcaggggca gtcgttgtag
     5941 atagctacag acaaaaacgt gaaaaatatg attccaagac cttcgtcggc tctggtaagt
     6001 tggaagagat tgcgcttatg gtggatgcag aagaaatcac tactgtcatc gtcaacaatc
     6061 gtctgacccc aaggcagaat gtcaatctag aggaagttct cggtgttaag gtcattgacc
     6121 gtatgcagtt gattttggat atctttgcca tgcgggctcg aagccatgaa gggaagctcc
     6181 aagtccacct agcccaactc aaatacctct tgcctcgctt ggttggtcag gggattatgc
     6241 tcagccgtca ggcaggggga attggttccc gtggtcctgg tgaaagccaa ctggagctga
     6301 accgtcgtag cgttcgcaat caaatcacgg atatcgagcg ccagcttaag gtggttgaga
     6361 aaaatcgtgc gactgtcaga gaaaaacgtt tggagtctag cacttttaag attggtttga
     6421 ttggttatac caatgctggg aaatcaacta tcatgaacat cttgaccagt aagacccagt
     6481 atgaagcaga tgagctcttt gcgactctgg atgcgacaac caagagtatt catctgggag
     6541 gcaacctcca agtaactttg acagataccg ttggctttat ccaagatttg ccgacagagt
     6601 tggtgtccag tttcaagtca accttggaag aaagcaagca tgtggacctt ctggttcatg
     6661 ttatcgatgc tagcaatcct taccacgagg agcatgaaaa aacggttctc tccatcatga
     6721 aagacctgga catggaagat attcctcact tgacgcttta taataaagcg gatttggtgg
     6781 aggatttcac gcctacccaa acgccatata ccctcatttc tgccaagtct gaggacagtc
     6841 gtgaaaactt gcaagcatta ttgctagata agattaagga aatttttgaa gcatttaccc
     6901 tgcgagtgcc tttttcaaag tcctacaaga ttcatgattt agagagtgtt gcaattctgg
     6961 aagaacgtga ttatcaggaa gacggcgaag tgattacagg ctacatttcc gagaaaaata
     7021 aatggaggtt agaagaattt tatgactgat attaaaactt tggctctaaa gtatggaggt
     7081 tatacaagtc tggacaaggt ctatctggat cagcttctag ctggtaaaac agatcaggag
     7141 cagttaacac ttattacacc tccgccaagt gttgttaatg cctactttgc agaactctat
     7201 cagaaaaaga gtcctcaggc tgcgacggat tattttgcag aactcagtca ggaactgaat
     7261 ctttacaata ctgagccaag tttcacccta gaaaataagc cttttattcg gcttaatctg
     7321 tctggtaaat cctttggttt ttgttatgag agtgacggcc ttggtcggat tttctttgaa
     7381 aatgaggaga aaatctcgga tgacttgctc tttgaaattg cgcaaatttt cccccatcaa
     7441 ttagtctttg aagagtctgg taagatttac atgaagcctg tcgaggacga ggaagttatc
     7501 agcctagaaa gtctcacagc tttgactgat ttggaaagat tggctgatgg tcgaaaacgt
     7561 ctcaaaggct acagccaaga ggatttattg caagaagctg ctgctttttc tggcaaacgc
     7621 tatttccgat cggaaaaccg cacagccatg ttatatattg attaattaga aagtatcgaa
     7681 tggatattca atttttagga acgggggctg gtcagccctc taaagcccgc aacgtttcaa
     7741 gtctcgccct gaaacttttg gatgagatta acgaagtttg gctctttgac tgtggagaag
     7801 gtacgcaaaa tcgcattctg gaaaccacaa ttcgaccacg taaggtcagc aaaatcttta
     7861 ttacccatct gcatggagac cacatttttg gtttgccagg tttcctttct agccgtgcct
     7921 ttcaggccaa tgaagagcag acagatttgg aaatctacgg acctcaagga atcaagtcat
     7981 ttgtcttaac cagccttcgt gtgtcaggtt ctcgtctgcc ctaccgcatt catttccatg
     8041 agtttgacca agattctctg ggtaaaattc ttgaaaccga taaattcact gtgtatgcag
     8101 aggagctgga ccacactatt ttctgtgttg gctatcgtgt catgcaaaag gatctagaag
     8161 ggacgctgga tgctgaaaaa ctcaaggctg ctggtgttcc gttcggcccg ctttttggta
     8221 aaatcaaaaa cggccaggat cttgttttgg aagacggaac tgaaatcaag gcagcagact
     8281 atatctcagc gccacgtcca ggtaagatta tcactatttt aggagacact cgaaaaacgg
     8341 gtgccagtgt gcgtctggct gtcaatgcag atgtcctagt tcatgagtcc acttatggca
     8401 agggtgatga aaaaattgct cgtaaccatg gtcactcaac taatatgcaa gctgcacaag
     8461 tagcggtaga agcaggtgcc aaacgcctcc tactcaacca tatcagtgcc cgtttcctct
     8521 caaaagatat tagcaaactc aagaaagacg ctgccacaat ttttgaaaat gtccatgtgg
     8581 tcaaagactt ggaagaagtg gaaatctagc agtcacagaa aggataagta tgcctactat
     8641 tctcattacc ggagctagcg gtggtctagc tcaagaaatg gtcaaactcc tgcccaatga
     8701 ccaactcatc ttgcttggta gaaataagga aaaattagcc caactctacg gaaattattc
     8761 ccatgcagaa ttgattgaaa ttgatattac cgacgattca gccctagaag ctctggtaac
     8821 tgatctttat ctccgctatg gcaagattga tgtcttgatt aacaacgctg gttacgggat
     8881 ttttgaggga tttgaccaga ttgctgataa agatattcac cagatgtttg aggtcaatac
     8941 ctttgccctg atgaatctgt ctcgtcacct tgcggctcgt atgaaggaaa gcagcaaagg
     9001 gcatatcatc aacatcgtca gcatggcagg tctaatagct actggcaagt ctagtcttta
     9061 ctcagcgacc aagtttgcgg ctattggttt ttcaaatgct ctgcgactcg aacttatgcc
     9121 ctatggagtc tatgtgacaa cagtcaatcc aggtccaatc cgaacaggat ttttcgacca
     9181 agccgaccca gatggaactt atcttaaatc ggttgaccgc ttcctgctag aaccagatgc
     9241 agtggctaaa aagattgtca agattatagg aaaaaacaaa cgagaactca atctcccgat
     9301 tttgttgaac ctagctcata agttttatac cctctttcct aagctagctg ataagttggc
     9361 aggggaaact tttaattata agtaaaaaga accaatgtgc aggttgttgc tagcctacat
     9421 attggttctt atcatttctc aactatcaaa ctgaacttct tctaggagat agtctataaa
     9481 gcgttcgccc atcttagata ggctggtttt ctcatgctgg atatagacca gctcgatcgg
     9541 gtcatcaata tccagtggga tagaaacgat attgtctccg tttaggttgc tgttcaaaat
     9601 ccctgtcgca atggtataac catccaaacc aatcaagaga ttaaagaggg tggcacggtc
     9661 actgaccaca atggatttct tgtggtgttc ttgagaaaga atctcttctg aaaagtagaa
     9721 ggagttgtgc gtcccttggt catagctgag gtaagggaaa ttctccaaat cagacagttt
     9781 caccttgtct ttctttgcca gagggttggt cttgctgaca aagatatgtg gttgcgctgt
     9841 gaagagatgg tgggctagca ggtgattgtc atccagcatc ttggttaaaa catcacggtt
     9901 gtaactgttt aagaagagga ccccgacctc actgcggaag ttcttgacgt cgtcgataat
     9961 ctcccaagtc cgagtttcac gaaggaagag ttcgtatttc tccatatcgc ttttcttgag
    10021 caaagagaca aaggcattga ccacaaaggc atagtgttga gacgaaacgc taaatagttc
    10081 gcggtgggcg acaggatttt tatagcgttc ctccagaagc tgggtctgct cgacaacctg
    10141 acgggcataa gagagaaact ccatcccatc acgggtcaag gtgattccct tgggattgcg
    10201 gataaagatc tcaatgccca tttcattttc caaatctcgc actgcattgg agagacttgg
    10261 ctgagtgata aagagttgct tggctgcctc attcatggag ccagtttcga cgattttgat
    10321 aatataatgt aattgttgaa ttctcatgtt tttattgtac catacttgcc ttagaatcgc
    10381 taaagaagat aggaaaataa ggagcattgt gtgagtctct tcttgaaaaa aacacaaaat
    10441 tttgctagaa tgagtcttat gaaaacattc tatgatgtgc agcaattcct caaacgattt
    10501 ggtattattg tttacatggg aaaacgctta tatgatattg aactgatgaa gttggaacta
    10561 tctcggattt acgatgcagg gttgatggat aaattagact atctagaggc agaagcagtt
    10621 cttcgcagag agcacaaggt agaattggat tatattgaga aaaatggaga aaagaactaa
    10681 tggttacttg gattttgtgg gcacttatac tagcaatgtt ggcgtggatg ggctttaact
    10741 atcttcgtat tcgccgtgcg gctaaaattg tggacaatga ggagtttgaa gccttgattc
    10801 gtacgggtca attgattgat ttgcgcgacc cagcagaatt ccacagaaaa catatccttg
    10861 gtgcacgcaa tattccttca agtcagttga aaactagtct tgcagccctt cgtaaagata
    10921 aacctgtcct tctctacgag aaccaacgtg cgcaacgagt tacaaatgca gctctttact
    10981 tgaaaaaaca aggtttttct gagatttata tcctttctta tggcttggat tcttggaaag
    11041 ggaaagtgaa gactagctaa ccttatttcc aaattagtcg aatgatagga tgaagctatt
    11101 gttttatagt ataattaatt tgtataaaat ttaaggagtt cttagaacat aaaagaaaag
    11161 aagaaaaatc cactttttgt gggtatcttg tcgattattc ttggtttgtt atttccaatt
    11221 gtaggcctga ttttgggtat cataggattg gtcttggcta tttcatacca aaaa
//
LOCUS       DAVLSL010000059        11099 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLSL010000059 DAVLSL010000000
VERSION     DAVLSL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.060"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            7..999
                     /locus_tag="VXN30_001194"
     CDS             7..999
                     /locus_tag="VXN30_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1357821.1"
                     /translation="MNGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQKVI
                     EELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNAIVE
                     YFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVY
                     VSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVI
                     YGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFD
                     NIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            complement(1172..2125)
                     /locus_tag="VXN30_001195"
     CDS             complement(1172..2125)
                     /locus_tag="VXN30_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1357822.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     misc_binding    2457..2686
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2846..2926
                     /locus_tag="VXN30_001196"
                     /pseudo
     CDS             2846..2926
                     /locus_tag="VXN30_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            3655..5016
                     /gene="trpE"
                     /locus_tag="VXN30_001197"
     CDS             3655..5016
                     /gene="trpE"
                     /locus_tag="VXN30_001197"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1357823.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVINPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            5013..5579
                     /locus_tag="VXN30_001198"
     CDS             5013..5579
                     /locus_tag="VXN30_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1357824.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            5590..6594
                     /gene="trpD"
                     /locus_tag="VXN30_001199"
     CDS             5590..6594
                     /gene="trpD"
                     /locus_tag="VXN30_001199"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1357825.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            6591..7358
                     /gene="trpC"
                     /locus_tag="VXN30_001200"
     CDS             6591..7358
                     /gene="trpC"
                     /locus_tag="VXN30_001200"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1357826.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENRAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAEDVAQRIKELQIDKG"
     gene            7345..7944
                     /locus_tag="VXN30_001201"
     CDS             7345..7944
                     /locus_tag="VXN30_001201"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1357827.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAV
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            7922..9145
                     /gene="trpB"
                     /locus_tag="VXN30_001202"
     CDS             7922..9145
                     /gene="trpB"
                     /locus_tag="VXN30_001202"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1357828.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            9138..9914
                     /locus_tag="VXN30_001203"
     CDS             9138..9914
                     /locus_tag="VXN30_001203"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1357829.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(9886..10545)
                     /locus_tag="VXN30_001204"
     CDS             complement(9886..10545)
                     /locus_tag="VXN30_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1357830.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFCCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLSKVILITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            10615..11061
                     /locus_tag="VXN30_001205"
     CDS             10615..11061
                     /locus_tag="VXN30_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357831.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRVQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
BASE COUNT         3339 a         2108 c         2579 g         3073 t
ORIGIN      
        1 aaaaatataa atgggaaaaa ggtaactatt tatgatattg cacgtttatc aggtttttct
       61 ccaaaaacag tttcacgagt tattaatggt ggagtaaatg ttaaagaaga aacttatcaa
      121 gcgattcaaa aagttataga ggaattgtcg tatattccaa atgcgtatgc aaaaaattta
      181 actaaaaaag aagctataaa tatattgatt tctgtaaaaa aaatcgactc ttttccttta
      241 atttggtttc atacgctgtt agataaagtg ttacggacgt gtaaagaatt tggtgttaat
      301 gctatagttg agtactttgg agaggaggat acaattagta attcaattat atcaagcaca
      361 ggtagcctag tagatggtgt tattgttttt tatgaaagtg tagatgatat cagaattcag
      421 tatttaaaga agaaccatat gccttttctt gttttcggtg aatctcaaac gtctggagta
      481 gtctatgtat ctaataataa ttttcaagct acttatgata tgatgaaagc tgtaactgaa
      541 gaaaaattta aaaatatgtg gttgcttatg ggaggagaat cccatgttaa taaggatcgt
      601 gaaagaggtg tgcgttcttt tctgaatgat aaaaattact ttatggattt aaaagtcatt
      661 tatggcttat ctacaattga ttcagtttat tcttatgcaa tgcagcactt gactacccaa
      721 aattatccgg acattatttt tgtttcagga gatgaaaagg ttcagggctt gattcgtgcg
      781 tgctatgaga aaggaattct tattccagat gatatttcta tcattggatt tgataatatt
      841 cctatttctc agtattatac tccagctttg tctacaattg ctcccaatta tgttaaattg
      901 gctaaagaaa tgatagaagg tgttttagca attattaaag gggaaagtgt cacatctgtt
      961 gaagtttctc ctaaatttgt taggagacag agtttctagt attgtttaat tcttgaaaat
     1021 cctagtggaa aagatggagt gttctaaaat aagttctgtt tagcctccat tcagtttcta
     1081 ttgagttgga tccaatttct aacaagtggg acatgttcat gttcgatcta aggaagacat
     1141 ggtcacattc gacccaaatt tcaagttgaa gttagccacc caaaagaaat tcacttggcg
     1201 acttgtagtc caagcatttt ttagggtagt tgttaatcca attttcgata aaagctactt
     1261 ctttcggagt cgttttcttg gttcctttag gtaaccatct ccgaattaat cgattatgat
     1321 tttcatttga acctctctcc catgaagagt aagcatgtgc gtagtagata tgttcctcag
     1381 gaaatacctc agacaatcgt ttgaactccg aaccattatc tgcagtaatg gacagaatac
     1441 gatgctcccc taatagtaac gtaagcgcct gattgacaga ttcagcagtt ttatttggaa
     1501 ttaaccttat aatttggtgt ctgctccgcc ggtcggttaa gactaacagg caataatttt
     1561 ttatcttagt cagtaggacg gtatcaattt cataatgacc attttccaag cgaagattaa
     1621 taacgtcagg acgttcttcg atagatttac ctgccggctt aaagttcgga ctagcttgct
     1681 tcttgacacc tttccttttt ctgggataaa gcatgtcggc cttcgtcaat cctaaatgac
     1741 cattatgaaa ccagtagtag atggttgaaa taccaacttt cacttgcttc ttgttaacca
     1801 tcatttcagg cgaaaatttt tgcttatgat agtgtaagat cttctctctg atttcctttg
     1861 ttaaaattaa ctttttcacc gaccgttttc gattgaattg gtaaacagtt tgtgcgtaat
     1921 cggcagaata gacctttttg tatagccctt ttctcacttg ttgtaaagtt gtacctcttt
     1981 tgacttcagt atgaatcgtt tgaggcgcct ttcctaacaa gcctgcaatt tcacgattgg
     2041 acttattttc attcttccac cgctcaatca agcgacggtt atctattgtc aaatgtttcc
     2101 cttttggggt ataatgttct tgcatctctg tgcctttcct tgtgtttgtg gtgaacaaca
     2161 agtatagcac agaggtgttt tcttatacct taaatcacat ttcatttgac aacaggaacc
     2221 cttgaacctg ttttttcagc taaaccaagg ctatgattag ccttggccac ctagcctaac
     2281 agtttcaaac ctgttatcaa atgaaatcat caacttactc aaacatagtg aaacatctaa
     2341 ttcttgacta aggtaaggtg gctaacttca ttatagaact ttcaatggtc acattcgaat
     2401 gatattttca aaaaaatcgt aaaaaagtct tgacaaagaa aaaacaaagt attaaaataa
     2461 gttcaacaaa tcagaaaagt aactattttt gatcttcagg gagcctgtgg tggttgtgaa
     2521 taggtggttg gaagtagtga aagtgggctg attttgaaaa tgaatttgaa acaatgaaaa
     2581 ttcggtgcgc acaccttaca gtgcagcttg ttgttaaaca aggcagagat gtaaagggat
     2641 agtcccttta taattgaggt ggcaccgcgt taccaacgcc ctcacacgga agtatattct
     2701 gtgtgtgggc ttttttctat ccgtcgtttg gtttatcttt tattagggcc ttaagtcgct
     2761 ttgatgaact tgagttctat ctacagctcc tttcctccta ctaaaagcaa acaaaaagct
     2821 ggactaatac gaaaaaagct agtgatactc aatgaaaatc aaagaacaaa ctaggaaact
     2881 atccgcaggt tgctcaaaac actgttttga ggttgtggat aaaactgacg aagtcagctc
     2941 aaaatactgt tttgaggttg tggataaaac tgacgaagtc agctcaaaat actgttttga
     3001 ggttgtggat aaaactgacg aagtcagctc aaaatactgt tttgaggttg tggatagaac
     3061 tgacgaagtc agctcaaaac actgttttga ggttgtggat agaactgacg aagtcagctc
     3121 aaaacactgt tttgaggttg cagatagaac tgacgaagtc agctcaaaac actgttttga
     3181 ggttgcagat agaactgacg aagtcagtaa ccatacctac ggcaaggcga cgttgacgcg
     3241 gtttgaagag attttcgaag agtataaagg tgtaccgaga tgaacatctc ttcatcaatg
     3301 tctctggtcg tggagggaag gacctagctg cgattgcaga ctactagaag ctaaaaaata
     3361 atagatggaa aaattacagt tctttctctc acagagagct tgtggttgct gaaaacaagc
     3421 agagaaagct gtaaagttgg gttcatctga aacgagagaa gaaaacatca ttctcaaggg
     3481 agtgcccttt atcgcacagc ctgttacagg agatatctga gacaggaatg agagatagga
     3541 gaaatcctat aattgaggtg gcaccgcgaa tttcgtcctc acgcaagtta ttttgcgtgg
     3601 ggatttttca tacagtcgct acgaactaga attcgaactg aaagggaaat tacaatggaa
     3661 cgaatcattc atggagatgt cttatcacca atcttggctt atatgcgctt aaaggggcaa
     3721 cacaaggtta tcttagagag tattccgaga gacaaggaaa cagctcgttt ttctatccta
     3781 gcctataatc cagtttttga gattaagttt gaaaatggag ttctctatca aaatggtcaa
     3841 gtgattgatc gggatccctt ggatttcctt tatgaagtga ttcataagag tcagcaccac
     3901 tcagagctcc cttttggtgg tggagccatt ggttttgtgg gttacgatat gatttcgctt
     3961 tatgaagaaa ttggtcaaat ccctgaggat acaattggga cgccagacat gcatttcttt
     4021 gtctatgaga gctatatggt ctttgaccac aagaaggaga agatacatgt catagaggat
     4081 gcactttata gtgagcgcag tcaagaggcc ttggaaaaat ccttgaacca agtgcttgag
     4141 gagttacgca ttcctgctcc aaatgaattt gaagacttgg atctatctcc gttagacttc
     4201 aaaccccata tcgctcctca gaagtttgag gaaatggtgg aaaccgctcg cgacttgatt
     4261 cgtaacggag atatgttcca atgtgtactc agtcagcgct tctcagcaga agttactgga
     4321 aatccatttg acttctacag aaatctccgc gtgattaatc cttctaatta cctttatttc
     4381 tatgattttg gggattatca aatcatcgga gctagtccag aaagtttggt ttctgtcaaa
     4441 aatggcatcg tgacaaccaa tccgattgca gggacgcgac caagaggggc tacggatgaa
     4501 gaagacaagg ccttggcgac tgacctcctc tctgatgaga aggaaacagc agaacatcga
     4561 atgttggtag acttggggcg taacgatatt ggccgcatct ctgaaacggc cagtgtccaa
     4621 gttactaagt atatggaggt ggagctcttc cgctacgtca tgcatttgac cagtgtggtc
     4681 aagggacgtt tgcttgcaga actcactgcc atggatgcct tgaaagcaac gcttcctgct
     4741 ggaaccgttt ctggagcacc aaaaattcga gctatgagac gcatctatga actggaaacg
     4801 gaaaaacgga gcgtatatgc aggagcaatc ggctacttgt ctgcgacggg tgatatggat
     4861 ttggccattg ccatccgaac catgattctc aaaaatcaaa gagcctatgt gcaggctggg
     4921 gcagggattg tctacgactc tatcgcccaa aacgaatacc aagaaaccat taacaaagca
     4981 aaatctatga ctagaattgg agaactaaga ccatgatttt attgattgac aactatgatt
     5041 cttttaccta taacttggcc caatacattg ggaattttgc agaagttcag gttctgagaa
     5101 atgatgattc caagctgtat gaagaagctg aaaaagcaga tggtctggtc ttttctcctg
     5161 gtcctggttg gccagttgat gctggaaaga tggaagacat gattcgtgat tttgcaggca
     5221 agaagccgat tctagggatt tgtttgggcc accaagccat tgcagaagtc tttggtggta
     5281 agctaggttt ggctccaaaa gtcatgcatg ggaaacagag caatatcaac tttgaagcgc
     5341 catctgtttt gtatcaaggt attgaggatg gccgtgcggt catgcgttat cacagtattt
     5401 tgattgagga aatgccagaa gactttgaag tgacagctcg ttcgactgat gaccaagcca
     5461 tcatggggat tcaacataaa aacctaccga tttatggctt ccagtaccat ccagagagca
     5521 ttggaacgcc agacggcttg tcttctattc ggaattttat cgagaaggtt gtaaagtgag
     5581 gaaactagga tgaaagagat tattgaaaaa ctagcaaaat ttgaaaattt atcaggtgtg
     5641 gaaatgacgg atgtcattga gcgtatcgta actgggcgtg taactgaagc gcagattgct
     5701 tctctcctct tagctcttaa gatgaagggg gaaacacctg aagaacgcac agccattgcc
     5761 caagtcatga gaggacatgc ccagcatatt ccaactgaga ttcatgatgc catggacaac
     5821 tgtggtacag gtggggacaa gtctttcagt tttaatattt ccacaactgc agcctttgtc
     5881 ttggctggtg gcggtattca catggccaag catggtaacc gctcgatttc ttctaaatct
     5941 ggttccgcag atgtcctcga agccttggga atcaatcttg acctcaaacc agctgaacta
     6001 ggtaaggtct ttgataaaac tggaatcgtc tttctcttcg ctaaaaatat gcacccagct
     6061 atgaaataca tcatgccagc tcgtttggaa ttgggaattc caacgattat gaacttgact
     6121 ggtccactga ttcatccaat ggctttagaa acacagcttc ttggaattag tcgtccagaa
     6181 ctcctagaaa gtacagctca ggttttgaaa aatatgggtc gcaaacgtgc catcgtggtt
     6241 gctggaccag aaggattgga tgaagctggc ttgaacggaa caaccaagat tgcacttctt
     6301 gaaaatggcg aaatcagctt gtcaagcttt actccagagg atttgggaat ggaaggctat
     6361 gctatggaag atattcgtgg tgggaatgct caggaaaatg cagaaatttt gcttagcgtt
     6421 ctgaaaaacg aagcaagtcc attcttggaa acgacagtct tgaatgctgg tcttggtttc
     6481 tatgctaatg gtaagattga tagcatcaag gaaggagttg ccttggcccg tcaagtgatt
     6541 gctagaggca aggcccttga aaaactcaga ctgttacagg agtaccaaaa atgagtcagg
     6601 aatttttagc acgaatctta gagcagaagg cgcgtgaggt ggagcagatg aagctggagc
     6661 aaatccagcc tctgcgccag acctatcgct tggcagaatt tttgaagaat catcaggacc
     6721 gcttgcaggt aatcgctgag gtcaagaaag ctagccctag tttgggagat atcaatctcg
     6781 atgtggatat tgtgcaacag gcccagactt atgaagaaaa cagagcagtg atgatttcgg
     6841 tgttgacaga tgaagttttc tttaaaggac atttggatta tctacgggag atttccagtc
     6901 aggtagagat tccgacgctc aacaaggact ttatcatcga tgaaaagcaa atcatccgcg
     6961 ctcgcaatgc aggtgcgaca gtcatcttgc tcattgtggc agccttggca gaagaacgtc
     7021 ttaaggaact ttacgactac gcgacagtgc ttggtctgga agtcttggtg gaaactcaca
     7081 atctagctga actagaagtg gctcacagac ttggtgccca gattatcggg gtcaataacc
     7141 gcaacttgac cacctttgag gtcgacttgc agaccagtgt agacttggct aaacatttca
     7201 aagatgattg cttgtacatt tctgaatctg ctattttcac agggcaggat gcggaacgac
     7261 tagccccata ctttaacgga attttagtag ggacagctct catgcaggca gaggatgtgg
     7321 cccagagaat caaggagttg cagattgaca aaggttaaga tttgtggact atcgaccaaa
     7381 gaagcggtgg aaacagccgt ttcagcagga gccgactata tcggttttgt ctttgcacct
     7441 agtaaaagac aggtgacttt agaagaggca gctgtgttgg caaagcttat tcctgcagat
     7501 gtgaaaaagg ttggagtatt tgtttcacca agtcgggtag aactgctgga agcgattgac
     7561 aaagttggct tggacttggt tcaagttcac ggtcaggtgg cagatgattt atttgagaat
     7621 ttgccttgtg ccagtattca ggctgtgcag gtagatggaa atgggcatgt ccccaattct
     7681 caggcagatt atctactctt tgatgcccct gtggcaggaa gtggccagcc ctttgattgg
     7741 ggtcaactgg atacgactgg actagcacag cccttcttta tcgcaggtgg ccttaatgaa
     7801 gataatgtag taaaagcaat tcaacacttt actccctatg cagtagatgt atcaagcgga
     7861 gtggagacag atggacaaaa agatcatgaa aagattagaa gatttataga gagggtaaag
     7921 aatggcatat caagaaccaa ataaagatgg attttacgga aaatttggcg gacgttttgt
     7981 cccagaaaca ttgatgacag cagttttgga gttggagaag gcctaccgtg aaagtcaggc
     8041 agacccaagt ttccaagagg aattaaacca acttttgcgc cagtatgtgg gacgtgaaac
     8101 tcccctttac tacgcaaaaa atttgaccca gcatatcggc ggagccaaga tttatctcaa
     8161 acgggaagac cttaaccata caggagccca caagattaac aatgccttag gacaagtttg
     8221 gcttgccaaa cgcatgggta aaaagaaaat tatcgcagaa acgggtgctg gtcagcacgg
     8281 tgtggcaact gcaactgctg cggccctctt taacatggaa tgtaccatct acatgggtga
     8341 ggaagatgtc aaacgccaag ccctcaatgt cttccgcatg gagcttttgg gagccaaggt
     8401 cgaggctgta acagatggtt cgcgcgtgct caaggatgcg gtcaatgcag cccttcgttc
     8461 atgggtggct aatatcgacg atacccacta tatccttggt tctgccttgg ggcctcatcc
     8521 atttccagaa atcgttcgtg acttccaaag tgtcatcggt cgagaggcta aacaacagta
     8581 ccgtgacttg acaggtcgag atctgccaga tgccctagta gcctgtgttg gtggtgggtc
     8641 gaatgctatc gggctcttcc atccctttgt agaagatgag tcagtagcta tgtatggaac
     8701 tgaagcagca gggcttggtg tggatacaga gcaccacgca gctaccttga ccaagggtcg
     8761 tccaggtgtc cttcacggtt ccctcatgga tgtgctccaa gatgcccatg gtcaaattct
     8821 tgaagccttc tctatctcag caggtttgga ctatcctggt atcggtccag agcattctca
     8881 ctaccacgat attaaacgtg ccagctatgt ccctgtgaca gacgaagaag ccttggaagg
     8941 attccaactc ttgtctcgtg tggaagggat tatcccagcc ttggaatcta gccatgctat
     9001 cgcctttgcg gtgaaattgg ccaaagaact tggaccagaa aagtctatga ttgtctgcct
     9061 atcaggtcgt ggggacaagg atgtggttca agtcaaggac cgcttggaag cagatgcagc
     9121 aaagaaggga gaagctcatg cctaagacac taacagaaaa attaaatgct attaaagcag
     9181 ctggaaaagg aatttttgtt ccctatatca tggctggtga ccacgagaaa ggtttggatg
     9241 gactaggaga aacaatccac tttttagaag atttgggtgt ctctgcaatt gaagtgggta
     9301 ttcccttttc agaccctgtt gcagatggac ctgttatcga agaagctggc ttgcgcagtt
     9361 tagcccacgg gacctctacc caggctttag ttgaaacctt gaaaaccatt gaaacagaga
     9421 ttccactggt catcatgacc tacttcaacc ccctctttca gtacggtgtg gagaactttg
     9481 tcaaagatct ggctgataca gctgtgaagg gcttgattat cccagacctg cctcatgaac
     9541 atgccaactt tgtagaacca tttttggcaa atacagatat cgccttgatt cctttagtaa
     9601 gcttgaccac aggaattgag cgccagaaag agttgattga aggggcggag ggattcatct
     9661 atgccgttgc catcaatgga gtgacaggga aatcaggcaa ttaccgtgca gatttggaca
     9721 agcacttggc gcaacttcat caagtggccg acatcccagt cttgacaggt tttggtgtat
     9781 ctagtcaagc cgatctggaa cgcttcaatg cggtgtcaga tggtgttatc gtcggttcga
     9841 aaatagtaaa agctctccac caaggagagc cgattcagga ctttatcaaa caagcagtag
     9901 cttaccaaaa ataatcacac aagcagcaag taagaggaaa ggcacgaaag gaagtctttc
     9961 ctttttcttt tgcaggagaa aggccaggat acccgtcgca gaagcgaact gaatcaagat
    10021 cagtaactcc gttacgctaa agacgagagc gcaagaagct aaaaaaagga aatcccctgc
    10081 acccatgcgg atatcgataa aatgagccaa aattccaagt atgaggaagg agaccatgac
    10141 cagattccag ccagaggaag ctattaggat taggtggaaa gtcatccaga ccagtaaggg
    10201 atattcctga tggcgaaagt cgtagatgcc caaggtcaaa ccagcagtga ttaggatgac
    10261 tttactcaag gaaagtaatt cccaagagta aagcagaaag aggagtccta agcctagttc
    10321 aaagagggca taccagacag gatagcggac cttgcagtag cgacagcaaa agcgattgaa
    10381 gacctgtgag agaatcggaa tcaaatctaa gggacgcaag cgagtctgac aggaatcgca
    10441 gtgactggct gaactgataa tggattgctc tggaaaacgg tcaatgacca aaccaagaaa
    10501 ggaagctaga atgctcccga cgagaaaaaa ataaaaatca atcatactta tctattcgta
    10561 aaaaatggga gaaatagtat aatggagaaa catagataag gtggtgagga aaagatgaca
    10621 attcgttttg aagaaaaggt gagcacagaa aacgctcaat ttgtatgcca gtggtccaac
    10681 tccctaggca aatcctttca agaacaatgg atgggaccaa ggattccttt tctactgacc
    10741 cttcaagcct tggaaggagt cttttcaatc tttgatgaac aagagtttgt gggatttatc
    10801 cagaaaatca ggctagaaga cagcaatctt catatcggga gattttttat caacccccag
    10861 aaacaggagc aagacttagg tagccaggct ttaaggaaat ttgttagttt ggcctttgaa
    10921 aatgaagata tagatagtat ttctctaaat gtcttcgagg caaatcaaag agttcagaat
    10981 ctttaccaaa aagaaggatt tgaaatcgtt caaatggttg aagcacctgt acgaaaatat
    11041 agtagattga aactagaata gtacacctct acttctaaaa cattgttaga aatcgattt
//
LOCUS       DAVLSL010000060        10988 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLSL010000060 DAVLSL010000000
VERSION     DAVLSL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.061"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..1070
                     /locus_tag="VXN30_001206"
                     /pseudo
     CDS             1..1070
                     /locus_tag="VXN30_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1198..2097)
                     /locus_tag="VXN30_001207"
                     /pseudo
     CDS             complement(1198..2097)
                     /locus_tag="VXN30_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            complement(2116..2937)
                     /gene="cbpG"
                     /locus_tag="VXN30_001208"
     CDS             complement(2116..2937)
                     /gene="cbpG"
                     /locus_tag="VXN30_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV1357832.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLSFIYSVLKGYSLEGWKKINGSWYHYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(3018..3257)
                     /locus_tag="VXN30_001209"
     CDS             complement(3018..3257)
                     /locus_tag="VXN30_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357833.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(3407..3673)
                     /locus_tag="VXN30_001210"
                     /pseudo
     CDS             complement(3407..3673)
                     /locus_tag="VXN30_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3698..4009)
                     /locus_tag="VXN30_001211"
     CDS             complement(3698..4009)
                     /locus_tag="VXN30_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002877158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1357834.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYKHPELEEIILIFFENLFKPRQLYLQPQNSVLSNLQLA
                     S"
     gene            complement(3934..4176)
                     /locus_tag="VXN30_001212"
     CDS             complement(3934..4176)
                     /locus_tag="VXN30_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1357835.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(4177..4809)
                     /locus_tag="VXN30_001213"
     CDS             complement(4177..4809)
                     /locus_tag="VXN30_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1357836.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4823..5818)
                     /locus_tag="VXN30_001214"
     CDS             complement(4823..5818)
                     /locus_tag="VXN30_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1357837.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5815..6513)
                     /locus_tag="VXN30_001215"
     CDS             complement(5815..6513)
                     /locus_tag="VXN30_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1357838.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRLMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6591..7601)
                     /locus_tag="VXN30_001216"
     CDS             complement(6591..7601)
                     /locus_tag="VXN30_001216"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1357839.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVLGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(7585..8592)
                     /locus_tag="VXN30_001217"
     CDS             complement(7585..8592)
                     /locus_tag="VXN30_001217"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1357840.1"
                     /translation="MIAVKTCGKLYLAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(8579..9532)
                     /gene="mvaD"
                     /locus_tag="VXN30_001218"
     CDS             complement(8579..9532)
                     /gene="mvaD"
                     /locus_tag="VXN30_001218"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1357841.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(9514..10392)
                     /gene="mvk"
                     /locus_tag="VXN30_001219"
     CDS             complement(9514..10392)
                     /gene="mvk"
                     /locus_tag="VXN30_001219"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1357842.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIASAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(10514..10843)
                     /locus_tag="VXN30_001220"
     CDS             complement(10514..10843)
                     /locus_tag="VXN30_001220"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1357843.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         3276 a         2517 c         1959 g         3236 t
ORIGIN      
        1 ataaagtctg acatgaaggc tggactaaag atagagtttc tcttgaatac ggcctcccaa
       61 gtcgtacgat acttcttaac tggctagcac aatacaggaa aaacgggtat actattgttg
      121 agaaaacaag agggagagta cctgagagcg gagaatgcca tcctaaaaaa gttaagagaa
      181 ctccgattga aggaggaaaa agagaaagaa gaaagacaga aattgttcaa gaattaatga
      241 ctgaattttc gttagctctt cttctaaaag tcattaaact agctcgttgg acctactact
      301 atcacttgaa acagctagat aaaacagata aggaccaaga gcttaaagct gaaattcaat
      361 ccatttttat cgaacacaag ggaaattata cttatcgtcg gatttattta gaactaagaa
      421 atcgtggtta tctgataaat cataaaagag ttcaagtctt gatgaaagta ctcaatttac
      481 aagctaaaat gcgacagaaa cgaaaatatt cttctcataa aggagacgtt ggtaagaaag
      541 cagagaatcc cattcaaggc caatttgaag gatctaaaac aatggaaaag tgctacacag
      601 atgtgacaga gtttgccatt ccaaatagca cacagaaact ctatttatca ccagttttag
      661 atggttttaa cagtgaaatc attgcttatc atctttctac ttcgcccaac ttagaacaag
      721 tgaagagtat gttagaacag gcctttacag agaaatacta tgagaatacg attctccata
      781 gtgaccaagg ctggcaatac caacacgatt cttatcatcg gttcctagag agtaagggaa
      841 ttcaagcatc catgtcacgc aagggcaaca gccaagacaa cggtatgatg gaatctttct
      901 ttgggattct gaaatcggaa atgttttaca gttatgagaa gtcgtttcag tcgcttaagc
      961 aattggaaca agccattata gactatattg attactacaa caataaacga attaagataa
     1021 aactaaaagg acttagccct gtgcaataca gaactaaatc cttcggataa attatttgtc
     1081 taactgtttg gggtcagtac agaaaaactt tgatactatg cgttttttga ttgtgaagac
     1141 ttttatccac ctattataca aagttaagta tccatcacag tttacaatta gtcttcatta
     1201 cttaacccat tcaccattat agtttaaata gtaaccacct actgtggtat taacagctaa
     1261 agcacctgaa tcataggtat agtaccagtt accattgact tggaaccagc ctgttttcat
     1321 atctccatta cttgcattta gatagtacca agtcgaacct tcctgatacc aaccagttgc
     1381 catagctcct gatgaacgga gatagtacca tttgttccca aggttttgcc aaccagtttg
     1441 cataatgcca gttgttggat ctagatagta ccatggagct gcttttagct tctcttcatc
     1501 atacgtaaga ggggaatcct taacccaacc acgtgctagc tctccaaccg ccaatctgtt
     1561 gatgcgcgaa tcaaagccac catccttctg taaataatac caatgaccct cttcgtaaat
     1621 ccaaccagtt tttaaagtat gataactacg ctgatcttca aaataataga ctcatttctc
     1681 tgctgggcta tcatattctt ccccatgatg tttgttggta ttcgtagcag tttttgcttc
     1741 taaaacttgc ttgccaacaa attcttgtaa tacaccatct tgaccaaaat aaaaccaatc
     1801 tggtctaaga gaaatctcta ttcttggaga aggaccaatc gtacccccct tgtgtggagc
     1861 aggtatatat tgccagccga caaccatctc tccggataaa ggatcaaaat aatagtactt
     1921 cccatctatc actctccagt aggtttcttt tagatccccc tttttgtagt aggttctacc
     1981 attttcttgg acaaactgcc atccttcaga atcatctgca aatactgtac tggtccctag
     2041 caaaccgaag aaaaatgtgg ctagtgcgac ttgcatcatt tttttcaaaa gcttcatttt
     2101 atatatcctc caacattaaa tccactcacc agatgaagcg aagttataaa ctttaccatc
     2161 gatagtttgg ctacctgtaa ccattgctcc atttgaattg agataatacc attttccatt
     2221 tactttttgc caatctgtaa gcatcttacc ggaactgtct aaataatacc aagtatcatt
     2281 tatctcctgc caacccgttt gtttatcatg ttgtctataa tggtaccaac taccatttat
     2341 tttcttccat ccttcaagag agtaaccttt aagaaccgaa taaataaatg acaaatttcg
     2401 ttcatttaat ttaactgcac tgttaatttg attagctcca tctcctaaag tatgcactcc
     2461 tactacgcgg tgactagcat cataaactgt agatccacta gacccctcta aagtatcaac
     2521 ttggtaatcc aagaacatgc catcatcact taaaacttgt tttttatctg tatacatttg
     2581 atgaatttta aaattatatg atggatagcc tgtgatagtc acagttattc ctgtcaaatt
     2641 tttttgacta gtaggaagac ccaaagtccc taattttgca ccaatgggct cttctagaat
     2701 taataaagcc aagtcatatt cccttgcatc cttagaattt aaatttctaa attccttcaa
     2761 ataacgaact tcctttactt tgatctttcc aaatagttct tgacttggac taacagccgg
     2821 aagaacataa atatcatccg cttctttccc atagtcatgt ctgtagtagt tgtgagccgc
     2881 tgtaattaac acattatctt taataaaatt cgcacttccc ctcccttcta cattcattcc
     2941 atcggctact ccataatact tagataaaac catagctgaa gtcgaatacg gatactgtaa
     3001 agtattatca attttaatca aatcatcatt accgataata cttctgattg cttttggtag
     3061 tatgaaccat acgttggtga aatctcagat aatgaagaat cattagactc tggacctttt
     3121 tctagtgtct cacttacctc atattcttca cctgtactag aaataacact caaagcagat
     3181 actgtcgata actggctagc caataaagta ctcgcaataa ttgaaatacc caatttttta
     3241 taaacagttt tcttcattat tgtatcctcc taatgtaatt atagcgtact attctaaatt
     3301 tcttaatcta ctatagaatc aagaaatcta ccaccttctt taaataccct ccattatcac
     3361 ataaacaggt aaacttttca attaatgact gcgcttttca atcacgctag aggtacttac
     3421 ttgcttcttt gatactaagt tcagccattc tttccttgtt tttctcaata aagcatgtta
     3481 cccaagtggg attcgttttg gagtagtctc gcagagtcca gccaatggct ttattgataa
     3541 aaaattctgt ttggttcaag ttatgaagta gaatcttttc cattaattga gtattggtct
     3601 tctcttttct taacaactgg tggtcaatag cgacacgtct cagccagata ttatctgata
     3661 ggctccattt tatactcaat gaaaatcaaa gagcaaacta ggaagctagc tgcaggttgc
     3721 tcaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga ttttcgaaga
     3781 atattaagat tatttcttct agttcagggt gtttatacac caaactccct actactcgat
     3841 ctaggatatc taccgtgtcc caccaggatt ttgtcacgac taactgctct agcttaggca
     3901 aatcggtttc ctttagataa gactgcattg ctttcaaata gttagcagcc acatattggt
     3961 attttctagg ctccttttcc cagcaagtgt ctgcaaaatc ccaatcgata atctttgttt
     4021 ttttcgcttc tggaaaatat ttttatagag tttatttctt tcaggcaccg caatacctag
     4081 aaaagaaaat tgatggcgca tataggcttc catggacctt gcttttttag agtcttttgc
     4141 tgcttctagc tcctcaagta aatctgctaa actcatctaa aactcctctt gccccaccaa
     4201 atggtgctga aaggcataga cagccgcctg ggtacgatcg ctgacttcaa gtttggcaag
     4261 aatattggac acgtgggtct tgaccgtctt gagagagata aagaggtcat ctgcgatgcg
     4321 ctgattttcg tagcccttgg cgatgagttg gagaacatct cgctcacgcg cagtcaattc
     4381 ttcatgaagt tccatatgat tgcggtggta ttcaaccttc ttgctaacct cttgctcaat
     4441 ggccagctcg ccagcagcta ccttactgac ggcatgaagc aattcatctg cactagaagt
     4501 cttgagcata tagcctttgg caccagcatc taagactggc atgatttttt cattgtccaa
     4561 ataagaggtc acaatcaaaa tcttggcttc aggccattct ttaaggattg ctaaggtcgc
     4621 gtcaatccca ttcatctcag gcatgacaat atccatgaca atgacatctg gacgcagttc
     4681 caaggccaag tcaatccctt gagacccgtt ggacgcctca cccacaactt ctacatcgtc
     4741 ttggaggtca aagtagcttt tcaagcccaa acggaccatt tcatggtcat ctactagtaa
     4801 aattttcatc tttactcctt tatcattcct tatctaacag gggaatacgg atatcaactg
     4861 ccaacccttg cttgggagct gttaataact gaaccgtccc tgccatatct tcaacccgct
     4921 ccttgatatt tcgcagtcca taactcaagt cgtctaagct ccctaactgg aaaccaatcc
     4981 cattgtccac caccttcagt tgcaattcaa catctgtctg atagaggtag acatctaggc
     5041 aagatgcctg ggcatggcgg agcgtattgc taatcaactc ttgcaggata cggaagatat
     5101 gctcctcgat tttcttaggc aatttcgtca tattctgctt gagactaacc ctaagatcac
     5161 tcttgtcctc aagctctttt aagagaattt gaatcccttc tatcaagctc ttctgctcca
     5221 gttcaactgg tcgcaaatgc aagagcaaaa cccgcaaatc cttctgggca gtttctaaaa
     5281 tagctgtgac actctgcaac tggatctgca tcttttctct atccaatttc aaagcctgct
     5341 gactgatacc cgataaaatc atgtgggccg caaacaactc ctgactgact gtatcgtgca
     5401 aatcccgagc aattcgcttc cgttccttct cgatgatttc ctcttcctga gcaaggctat
     5461 gattttcagc tttttgaaga gcttctgtca aaaggttaag tttacctgat aaggacttga
     5521 aactggcatc caaatctgga tctgcaacct gaaccacttc ttgccctgcc aataaacgct
     5581 tgagattagc ctgcattttt cttagagaaa gctcttcgat ccctcgccaa aacagggcta
     5641 agagacaggt tatggacatg ctgaaaacca acaataaaaa gacaaatttt tctgtttttt
     5701 cgacatcgtg caaaaagata gaccagtcaa aatcaagtat ttccagcaag ctgtgggaga
     5761 aaaaaaagac aaacaggaag gaggtgagag caataatgac ataggcttgt tttttcatcc
     5821 tctaaccacc tccacatcac caatcatagt ggtcaagaaa atcttgacac tcttgttact
     5881 cttgagatag tcttttgttt cttgatgata gtgttcattg cggagggctc gcttgggctg
     5941 gttgaaaaaa gtcaaatccc catagagaca gttaacgctg agactgactt ccacatctac
     6001 aggtacgatg attttggtcg ttcctaccat ctttctgagg ataatgacat tgtcatgatt
     6061 ggttaggatg accctctcca gatgaatagt gtccttgccc atgaggcgaa agagattgat
     6121 atcatcaaat tggcaagtct ggtagcttga aaaatgatga agatttccaa accaacgatt
     6181 tttctccttc ttaaccgtca caacctcttc aaaaaccaaa ttggtctgct ctttttcctg
     6241 gttcatcatc gggtaaagaa gaaagaggct atagataacc gcaacaaaaa tagctagaat
     6301 cacaaaagga ttgagcataa cgatgaaaaa gaagagaatg gttgccgcta ctaaaagaag
     6361 attatttccc tctttaccag tgtagtagcg aatcaaaagc aaaaagagga atagtatcag
     6421 cagaaaacgc gaaaaatgct ctgataccat caaaatcaga gctcctgtca gaagacaggc
     6481 ttcgataaat aaaaagattt taaattttct cataggttca tcctctccct tctattttat
     6541 cacaattcaa aaaagtcacc tcagtctgag gatggaaaaa aaggcgctgg ttacgccttt
     6601 ttcatctgat catttgcttc ttttaatttt ccataaagaa gatagtctac tttttgtaga
     6661 tctgctatgg tggcacagtt aagggaacac ataatcaagc gtagatctgc tttccagcct
     6721 tggacaatgc caagcacttc ttcaactgtg taggtttcaa ccaattccag aacggttcgt
     6781 gacaatccca cagccttagc accaaaaacc aagcacttaa tcatatccag cggattccga
     6841 acccctccac taaccaagag ttcgacctta tctttccatt cttgggcatt gagaagggcc
     6901 tgcatggtag actgacccca ttgattgagg taatcacgct ggccactacg acggttttcg
     6961 atataggcaa agctggtgcc accacgaccc gataggtcca ctgtacgaac accgaattca
     7021 taggctcttt cgattgtctt ggcatccatt ccaaagccca cttccttgag gacaatagga
     7081 acgggaattt gctcgctata atctgctaaa tgcgattgcc agcttctaaa cttcctttct
     7141 ccctcgggca tgagtaattc ctgcatgaca ttgacatgca cttgcaatag aacaggattc
     7201 atctcttcta cagtctgaag tcctaactcg acaggcttgt ccaatccaat attggttcca
     7261 aggaggagat tgggatgact agacttgaca gaaaaagaat catccgttgg atttttgagg
     7321 gctgcgctat aagaacccgt tacaaataaa ataccacagg attccgccac ctgagccagc
     7381 ttttgattga tttctcttcc cttattactt ccaccagtca tggcattgat ataaaaagga
     7441 aagtcccact ttcgaccagc aaactctgtc gaaagatcga tttcatccag attgtaaaga
     7501 ggcaaggaag aatgaatcag ctccacctca tcaaagctat tataggaact tttctgctca
     7561 agggcataga ggatatgctc gtccttacga tttgtcgtca tgtcctatcc tttcttgata
     7621 taagagctca atccccagat cggcccaacg attttttaag gttttggttg attgcgcatc
     7681 aaaactcagg gcgatgccac agtcaccacc accagcacca ctactcttgg caacggcctg
     7741 caaatcttga ctggcttctt tcaactgtct aagcaaaggc gtgtaaatat ctgtactcaa
     7801 gccttctaaa agcttgctgg ctacttctac ttgctcgata atcttttctg atttcccctg
     7861 ttccaaggct tcgaccaaag aaaccaccgt ttcttttgag gaagttaaaa aattttgatt
     7921 gatattttgc ttgatttgct gaaccatgtg actcgataca gccacttcct tggtccatcc
     7981 cactaagaaa tcacattcta aagttggttt cacttgtgag ataaaaaatc cccaatcacg
     8041 ctccagaact gtcgccaagt tttcttcttc taaccaagca gccgccttct ggcgatcaaa
     8101 tgactggtag agaaccaaat cctctgccac aatacaggca aggtcgccca tggaaccatt
     8161 gtctcctcgc ttgagcaaga cagcgctagt cagcttgaac aagagctcct gatcaacaga
     8221 aacatcatac agagccagta aagccttgac aaccaagaca acgacgctgc cactagaacc
     8281 tagaccaaac tttttccctt ctcgttccat tttgccacag atttctagag aaaaaggtct
     8341 taaattctga ccgcgaacag cgaggaagtc tcccatcaaa gcaatcgttt cttgaatcaa
     8401 gctgtagtca ggattgggcc ttaagtccac tgcgaaatca aacatatctg aatagatacg
     8461 gtagctgtca gaaaaagcaa tctcagccct catatagatg ggaatatcct ttatcaaagc
     8521 taactgccct ggctctaaaa tagcatattc acctgccaaa tagagttttc cgcaagtttt
     8581 aacagcaatc atcttgactc aaatcctttg tttttgacac aatcaagcga taacgctgac
     8641 cgaaaatttc tgacaaatgc tccaagtctt tctcctgaca gaagacctta acattgggac
     8701 cagcatccat ggtaaagtag caggcctctc ctttctcacg aagctgacga acaaaggcca
     8761 tagcctcata agaggcatcc gtcagataag aaaaggctgg actagcagtc tttgtcgtag
     8821 catgcatagc cagggcattt ttctccgtta attctccaat cttggcaaaa tcattttcct
     8881 tgagataaat cagcatatcc tgatagtcct tctcagactg acgaacccag tcgtcgaaag
     8941 tcgtcgaggt ttccacacaa agtttcatcc cgtcacggct agagattggt tttttcttgt
     9001 cctctagcac caacataatc atagctagtt tcaagtctgt ctctacaggg taaatttctc
     9061 cactatcctt atcccaggct cctagtggtc cataaaaact ccgagaagaa gagcctgagg
     9121 caaatttggc ttcctgtgcc aactgacttc tatccaatcc aagtttgaaa taagcattac
     9181 aagccttgac cagggcggac aaaccactag aacttgagga caggcccgct gccgtaggca
     9241 tattgttttg agtatcgata cggacaaagc cctcaccagc tggacgataa cggtcaataa
     9301 tcttactcat cttggcatgc tcgacctcat tttgtagctg accattgatg taaaattcgt
     9361 cagctgttac attagctggt aaaggcgaca aggtcgtctc tgtatacata ttttccaaag
     9421 ttagagaaat actgctagta gcaggcacca tctctttttc ttttttcttt ccccaatatt
     9481 tgataatagc aatatttgcg taggaacgta ctgttacagg ctctctatcc atgtctgaac
     9541 agctcctttc tcttctaatc tttctgctag ttcttgtgcg tgtgtcaaat tggttaccaa
     9601 ggctatgata caacctccta gcccaccacc gctcatcttg gcacccagag caccatggct
     9661 aagagtcgtt tcaaccaaaa agtctgcctc agggctactg actccaattt cttttaaatg
     9721 caaatgcgcc tgactgagga tttgtcccag tccttcagca tctttttgtg aaatcgcaac
     9781 ttctgcttgc tgggttaatt ctcccaaggc atgcaaaaac ggtagggcat ccttgccctt
     9841 attttgaacc acttggatgg cttcacgagt atgaccataa acacccgtat cggcaatcac
     9901 caaataggcg gataaatcca tctcaagttc tgtaaatcct acgttcttga taaagcgaat
     9961 aggttggtca ctgagacagg tcttagcatc caaaccacta ggattcatat gggcaatcat
    10021 ttcagctcga ttgaccaaga tttctagtac atcatgaggc agatcagcct gatagtagtc
    10081 aaataccgca cgaatggccg ctatgctgat agccgctgac gaacccatcc cccgtttctc
    10141 agggatagcc gaggcaatct cacaacgaat gcaggcttct gtgatatcca aatactccag
    10201 tgaggcataa accgccatgg acaaggtatc ctcctcataa aggcgccaag gactctctgc
    10261 aggaactacc ttacaggtca cctccacctc caaaagaggc agggaaatgg caggataacc
    10321 gtaaacgacc gcatgttccc ctattaaaat tatcttacta tgtgcctgac cgacaccaac
    10381 tttttttgtc attttttcct tttactagac gaaaaaacgt cttatttttc atacaagtat
    10441 taattctttc ctatctattt tattatattt tcacaaaaaa agcgattgtt tccattcaca
    10501 atcgcttctt tcattattga acccattcgc cattatagtt gacagaatag ccatctacgg
    10561 tcgtattcac tgccaaggca cctgagctat aagcgtagta ccatctgcca ttgacctgga
    10621 accaacctgt cgtcatagaa cgacgaaaga aactccatac cgttaagtaa agaggaaagt
    10681 cgtgagggat catgcgccat tgacaacctg ttttagtgac gtacaaagtc tcattaacaa
    10741 gtactcgttt cggccattta taggtgcggt gtttggagaa atagggttca atcttagccc
    10801 attcttgatc gtttaaatca gtatcatatg ctttgcgtgt cataactcta gtttaacatt
    10861 tttttgtgaa tacaggttct aaataatcga ccacgaaaat ttcttaagtg gaaaacgcct
    10921 tatgaagtat gctacgggaa agttatgcac ttaatttgac aattcaagat gtaaaaatat
    10981 atactata
//
LOCUS       DAVLSL010000061        10958 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLSL010000061 DAVLSL010000000
VERSION     DAVLSL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.062"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            51..1529
                     /gene="guaB"
                     /locus_tag="VXN30_001221"
     CDS             51..1529
                     /gene="guaB"
                     /locus_tag="VXN30_001221"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1357844.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(1580..2677)
                     /gene="recF"
                     /locus_tag="VXN30_001222"
     CDS             complement(1580..2677)
                     /gene="recF"
                     /locus_tag="VXN30_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1357845.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSVQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEKL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNRN"
     gene            complement(2680..3048)
                     /gene="yaaA"
                     /locus_tag="VXN30_001223"
     CDS             complement(2680..3048)
                     /gene="yaaA"
                     /locus_tag="VXN30_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1357846.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            3207..4457
                     /locus_tag="VXN30_001224"
     CDS             3207..4457
                     /locus_tag="VXN30_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1357847.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            4454..5737
                     /locus_tag="VXN30_001225"
     CDS             4454..5737
                     /locus_tag="VXN30_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1357848.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            5779..6600
                     /locus_tag="VXN30_001226"
     CDS             5779..6600
                     /locus_tag="VXN30_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1357849.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEVTVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            6611..7156
                     /gene="pgsA"
                     /locus_tag="VXN30_001227"
     CDS             6611..7156
                     /gene="pgsA"
                     /locus_tag="VXN30_001227"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1357850.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            7153..7980
                     /locus_tag="VXN30_001228"
     CDS             7153..7980
                     /locus_tag="VXN30_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1357851.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            7965..8804
                     /locus_tag="VXN30_001229"
     CDS             7965..8804
                     /locus_tag="VXN30_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1357852.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            8797..9591
                     /locus_tag="VXN30_001230"
     CDS             8797..9591
                     /locus_tag="VXN30_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1357853.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            9650..10468
                     /gene="mreC"
                     /locus_tag="VXN30_001231"
     CDS             9650..10468
                     /gene="mreC"
                     /locus_tag="VXN30_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1357854.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            10468..>10958
                     /gene="mreD"
                     /locus_tag="VXN30_001232"
     CDS             10468..>10958
                     /gene="mreD"
                     /locus_tag="VXN30_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1357855.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
BASE COUNT         3301 a         1661 c         2338 g         3658 t
ORIGIN      
        1 acaatataaa aagagtcaag ctcaaaaaga aagaaaagag gaaacttcga atgtctaatt
       61 gggacactaa atttttgaaa aaaggtttta cctttgatga tgtattgctt attccagctg
      121 aaagtcatgt gttgcctaac gatgcagatt taacaactaa attggcagat aatttgactt
      181 taaatatccc aattattacc gctgccatgg acacagttac agagagtcaa atggccattg
      241 ctattgctcg tgcaggcggt ctcggagtta tccataaaaa catgtcaatt gctcaacaag
      301 cagacgaggt tcgtaaggta aaacgttctg aaaatggagt tattattgat ccgttcttct
      361 tgacgcctga acatacaatt gctgaagcag atgagcttat gggtcgttac cgcatcagtg
      421 gtgttccagt tgttgaaaca cttgaaaatc gtaaattggt tggtattttg acaaaccgag
      481 atcttcgttt tatttcagat tataatcaac caatttcaaa ccatatgact agtgaaaatc
      541 ttgttactgc tcctgtgggt acggatcttg caacggctga gagtattctt caagagcatc
      601 gtattgaaaa acttccgttg gtcgatgaag aaggcagtct ttctggtttg atcactatca
      661 aagatattga aaaagttatt gagtttccaa atgcggctaa agatgagttt ggtcgtcttc
      721 tagttgcagg tgcagtaggt gttacttcag atacatttga acgtgcagag gctctttttg
      781 aggcaggagc ggatgcgatt gttattgata ctgcacatgg tcattctgca ggtgtcttgc
      841 gtaaaattgc cgagattcgt gctcatttcc cagatcggac tttgattgct ggaaatattg
      901 ctactgctga aggtgcacgt gccctttatg aagcgggtgt agacgttgtt aaggttggta
      961 ttggaccagg ttctatctgt actactcgtg tgattgctgg tgttggtgtt ccgcaagtaa
     1021 cagctatcta cgatgctgca gctgttgcgc gcgaatatgg taaaacgatt attgctgacg
     1081 gtgggatcaa gtattctgga gatattgtaa aagcacttgc tgcaggtgga aatgctgtta
     1141 tgcttggatc tatgtttgct ggaactgatg aagctccagg cgaaactgaa atcttccaag
     1201 gacgtaaatt caagacttac cgtggtatgg gatcaattgc tgctatgaag aaaggttcaa
     1261 gcgaccgtta tttccaaggt tctgtcaatg aagcaaacaa gcttgttcca gaaggaattg
     1321 aaggtcgtgt tgcttataaa ggagcggcag ctgatattgt tttccaaatg attggtggta
     1381 ttcgctctgg tatgggttac tgtggtgcag ctaaccttaa agaactacac gataatgctc
     1441 aatttattga aatgtctggt gctggtttga aagaaagcca tcctcatgat gtgcaaatta
     1501 ctaatgaggc accaaattat tctatgtaaa aaacaatgaa aagaactcca gtgaaaacag
     1561 gagttctttt acaatgttgt caatttctat ttacagcagc tttaccatcc tgaatagtga
     1621 agatacttag attttctggc aaattttgaa gatggtctaa gcttgttgtt gtgataaagg
     1681 tttggattga ttgagaaatc gtttctaata attttaactg tctagtgttg tcaagttcac
     1741 tcatcacatc gtcaagcagt aatatcggag attctgtggt aatgctttcc attaattcga
     1801 tttctgctaa ttttatcgag aggacgagac tacgatgttg accttggctt ccgaaactag
     1861 catccatccc atttatataa aaagaaatgt catctcgatg aggaccgaca ccagtattct
     1921 ttttaaataa atctctggat ctactttttt ctaaagcaat tttaaaagat tcggataagt
     1981 tttgtttgtc agttatattg acagaagatt gataggatat tgacaacttt tcgatctgat
     2041 tagagagttc aaaatgtttc ttacgcccaa atgattctag tttttttatg aaatctaagc
     2101 ggtgattcat tacacgacat ccataatcaa ctagctgatc atctaacaca gaaaggaatg
     2161 tttcatctat tttttgaact gattttaggt aagtgtttct ttgctttagg atgtggttat
     2221 aattggttaa gtcagataaa tagattggct taatttgccc aagctccata tcaatgaatt
     2281 ttcgtcgaat cgaaggtgct cctttaatta gttgtaaatc ttcaggagca aataagacaa
     2341 cattcatgtg tcctacataa tctgaaaggc gtgcctgttt taagtgatta acttttgtta
     2401 cacggccttt ttgtgttagt tcgatttcta ggggaatgga tccagttttt ttctgaacga
     2461 gacctgaaag atgaagttgt tcctcatcaa aatgaatgag atttttatct gttcgagttc
     2521 gatgactacg cgttaaggct aaaaaataga tagcctctaa catgtttgtt tttccttgtg
     2581 cattacgtcc caaaaagaca ttcaatttag gattaaagtc tattttcgtt tctttgtaat
     2641 tacgaaaagt cttgagagat agatgttgta gccacatgat tatctaccag ggaatcgtgg
     2701 agcttgttgg cttttaggtg atgaagcagg tttcgatttg tcttttttaa ctcccttatt
     2761 catctttttg acaagtttag cgatccgttc tttttcaact ttatcagctt ggtattcatc
     2821 ttgttcttca gaagtaggtt gtgtcaacaa gatgtcaatg ttcatgtcag ggatgtcaac
     2881 tttatcgcca atacgaagtt tttttccacg acgactctct aattccctat taaagtaaac
     2941 agaatgttca gagagaaatg atttaatagc tcctccgcta tgtgtaattc caagttcttt
     3001 gagtagtgct tggagggtaa taaattcttc aaataatttg tattccataa ttcacctcaa
     3061 tctttggtat tataccatat tttcctaaaa atagtgagag agacagagag aaatgtaagc
     3121 gatttttatt tcaaaagtgt tacagggaac aagaaaattt caggttttcg tgatataata
     3181 gaagtctgta tataaggagg taaatcatgg agttagtgca tggaatttca acacatttta
     3241 tccaatcaaa aaagtttaaa acaaacaaaa ttaccgtgcg ttttaccgct ccattatccc
     3301 ttgatacgat tgcaggtcac atgttgagtg caagtatgct agagactgct aatcagatgt
     3361 accccacttc tcaagatttg aggagacact tggccagtct atacggtaca gatatgtcaa
     3421 ccaattgttt cagaagaggg caaagccaca ttatagaatt gacatttacc tatgttcgtg
     3481 atgagttttt aagtaggaaa aacgtgctaa cctctcagat tttggaactt gtaaaagaaa
     3541 ctcttttttc acccgtagta gttgataatg ggtttgatcc ggccttattt gaaattgaga
     3601 aaaaacaatt gctagcaagt ttagcagctg atatggatga ttctttttat tttgcacata
     3661 aagaattgga taaattgttt tttcatgatg aacgtcttca attggaatat agtgatttac
     3721 gaaatcgtat tttagctgaa actccacaaa gttcttattc ttgtttccaa gaatttttag
     3781 ccaatgatcg aatagatttc tttttcctag gtgattttaa tgaggttgaa attcaaaatg
     3841 tattagaatc atttggcttt aaaggtcgaa aaggagatgt gaaggttcag tattgtcaac
     3901 cttattctaa tatccttcag gaaggtatgg ttcggaaaaa tgtgggacaa tccattttgg
     3961 aattaggtta tcattaccgt tctaaatatg gtgatgagca acatttaccc atgattgtaa
     4021 tgaatggttt acttggtgga tttgctcact ctaagctctt tacaaatgtc cgtgaaaatg
     4081 ctggattagc ttataccatt tcaagtgagc ttgatttatt tagtggattc ttgaggatgt
     4141 atgctggtat caatcgagaa aatcgtaacc aggctcgtaa aatgatgaat aatcaactgc
     4201 ttgatttaaa aaaaggttat tttacagagt ttgagttaaa tcagaccaag gaaatgattc
     4261 gttggtcgtt gttactttct caagataatc aatcttcatt gattgaacgt gcttatcaaa
     4321 atgccttatt tggaaaatct tcagcagact ttaaaggttg gattgcaaaa ctcgagcaag
     4381 ttgacaaaga tgctatttgt agagcagcta ataatgtgaa actacaggcg atttacttta
     4441 tggaaggaat agaatgacaa aggttgtttt tgaaaaaaaa tactatccag ctgtaaaaga
     4501 aatgatttat cgaactcgtt tggccaacgg attgacagtt gctcttttgc ctaaaaagga
     4561 atttaaagag gtttacggga gtgtcactgt acagtttggt tcggtagata cgtttgtcac
     4621 agaagttgac ggagatgtaa aacaatatcc tggaggaatt gctcattttc ttgaacataa
     4681 attatttgag agagaagatt ctagtgattt gatgtcggct tttacgagtc taggtgcaga
     4741 tagtaatgcc tttacaagct ttacaaaaac aaactatctt ttttcagcaa cggattattt
     4801 tttagaaaat ttagatttac ttgatgaatt ggtaacatca gcacacttta ctgaagcttc
     4861 cattctgaca gagcaggata ttattcagca agaacgagaa atgtaccaag atgatccaga
     4921 ttcgtgttta ttcttttcaa ctttagcgaa tttgtatcct ggtacacctt tagcaactga
     4981 tatagttgga agtgaggagt ccatttccca aatcaatcta actaatttgc aagaaaattt
     5041 tacaaagttt tacaaacctg taaacatgtc tctgttttta gttggtaatt ttgatgtgga
     5101 gcgagtacag gactattttg aaagcaaaga actgaaagat tcagattttc aggaagtagc
     5161 aagagaaaag ttgtttttac agcctgtaaa gccaacagat agtatgagaa tggaagtatc
     5221 ttctcccaaa ctagcgattg gagttagagg taagcgagaa gtttctgaag cggattgcta
     5281 tcgacatcat attttattaa aattattgtt tgcaatgatg tttggttgga cttcggatcg
     5341 ttttcaaaaa tgttatgaat caggtaaaat tgatgcgtcc ttatctctgg aagttgaaat
     5401 aacaagtcgc tttcattttg tcatgttgac aatggatacg aaagagccag ttgctttgtc
     5461 tcatcaattt aggaaggcta ttcgtaattt tacaaaggat ttagatatta cagaggaaca
     5521 tttagatatt atcaaaagag agatgtttgg cgaatttttc agtagcatga actctcttga
     5581 atttattgca acgcaatatg atgcttttga aaatggtgag acaatttttg atttgccgaa
     5641 aattttacag gaaattactt tagaggatgt ccttgatgct ggacatcatt taatagatga
     5701 tggtgacata gttgatttta caatattccc atcgtagtaa cctattataa tagacactag
     5761 aaagaaggga tgacaagtat gagaaaaaaa acaattggag aggttttacg attagctaga
     5821 atcaatcagg gattgagttt agatgaattg cagaaaaaga cagaaatcca gttagatatg
     5881 ttggaagcaa tggaagcaga cgatttcgat caacttccaa gtccttttta cacgcgttct
     5941 ttcttgaaaa aatatgcatg ggctgttgag ttagatgacc aaattgtttt ggatgcttat
     6001 gattctggga gtatgattac ttatgaggaa gtagatgttg atgaagatga gttgacaggt
     6061 cgtagacgtt caagtaagaa aaagaagaaa aaaacatcat ttttaccttt attttatttt
     6121 atcctttttg ctttatcgat tttaattttt gtgacttatt atgtttggaa ctatattcaa
     6181 actcaaccag aggagccttc tctttctaat tacagtgtgg ttcaatcaac aagttcaact
     6241 agctctgttc cccactcctc aagtagtagt tcttctagta tagaatcagc tataagtgta
     6301 tcaggcgaag gaaatcatgt agaaatcgct tataagacaa gtaaggaaac agttaaattg
     6361 caattggcag tttcagatgt tacaagttgg gtcagtgttt cagaaagcga acttgagggc
     6421 ggtgtaacct tatcgccaaa gaagaaaagt gcagaagtaa cagttgcaac taaaagtcct
     6481 gtaacaatta cgttaggtgt tgtaaaaggt gttgatttga cagtagataa tcagactgtt
     6541 gatttatcga aattaacagc tcagactgga caaatcactg taacctttac taaaaattaa
     6601 ggaaaaacga atgaaaaaag aacaaattcc caatctctta acaataggtc gaattctctt
     6661 tatacctatt tttatcttta ttttaacgat aggaaattcg atagagagtc atatagttgc
     6721 agctattatc tttgctgttg ccagtattac cgactattta gatggatatt tagctcgtaa
     6781 atggaatgtg gtcagtaatt ttggtaaatt tgcagatcct atggcggata agttactagt
     6841 tatgtcggct tttattatgt tgattgagtt aggtatggct ccggcttgga ttgttgcagt
     6901 gattatctgt cgtgagttag ctgtgacagg tttaaggctt ttattggttg aaactggtgg
     6961 aacaatttta gcagcagcaa tgcctggaaa aattaaaact tttagtcaga tgtttgccat
     7021 tattttcttg ctattacatt ggactttgct tggtcaagtt ctactttatg tagccttatt
     7081 tttcactatc tactctggct atgactattt caagggtagt gcctatgtat ttaaagggac
     7141 atttggttcg aaatgaaatc aataattgat gtaaaaaatc tttcttttcg ctataaagaa
     7201 aatcagaact actacgatgt gaaggatatt acgtttcacg tgaaacgtgg agaatggctt
     7261 tcgattgtag ggcataatgg tagtggtaaa tcaacgacgg ttcgattaat tgatggctta
     7321 ctggaagcag aatccggaga gattgtaatt gatggccaac gtttgactga ggaaaatgtt
     7381 tggaatatac gtcgtcaaat cggtatggtt tttcaaaatc cagacaatca atttgttgga
     7441 gcgactgttg aagatgatgt tgcttttggt ttggaaaatc agggactttc tcgtcaagaa
     7501 atgaaaaaga gagtggaaga agctctggct ttagttggca tgttggactt taaaaagaga
     7561 gagccagcgc gtctatcagg tggccaaaag caacgtgtgg ccattgcagg tgttgtagcc
     7621 ctaagaccag ctattttaat cttagatgaa gcaacgagta tgttggatcc tgaggggcgt
     7681 agagaactta ttgggacagt aaaaggaatt cgaaaagact atgatatgac ggtcatttct
     7741 attacccatg atttggaaga agtcgccatg agtgatcgcg tattggtcat gaaaaaaggg
     7801 gaaattgaat caactagtag tccaagggag cttttctctc gaaatgattt agatcaaatt
     7861 ggattagacg atccttttgc taatcaatta aaaaaatctt tgagccagaa tggctatgat
     7921 ttacctgaaa attatttgac agaaagtgag ctagaggata agctatggga attgctctag
     7981 aaaatgtgaa ttttacatat caagaaggta ctcccttagc ttcagcagct ttgtcggatg
     8041 tttctttgac gattgaagat ggctcttata cagctttaat tgggcacaca ggtagtggta
     8101 aatcaactat tttacaactc ttaaatggtt tattggtgcc aagtcaaggg agtgtgaggg
     8161 tttttgatac cttaatcacc tcgacttcta aaaataaaga tattcgtcaa attagaaaac
     8221 aggttggctt ggtatttcag tttgctgaaa atcagatttt tgaagaaacg gttttgaagg
     8281 acgttgcttt tggaccgcaa aattttggag tttctgaaga agatgctgtg aagactgcgc
     8341 gtgagaaact ggctctggtt ggaattgatg aatcactttt tgatcgtagt ccgtttgagc
     8401 tgtcaggggg acaaatgaga cgtgttgcca ttgcaggcat acttgccatg gagccagcta
     8461 tattagtctt agatgagcca acagctggtc tagatcctct agggagaaaa gagttgatga
     8521 ccctgttcaa aaaactccac cagtcaggga tgaccatcgt cttggtaacg catttgatgg
     8581 atgatgttgc tgaatatgcg aatcaagtct atgtaatgga aaagggacgt ttagtaaagg
     8641 ggggcaaacc aagtgatgtc tttcaagacg ttgtttttat ggaagaagtt cagttgggag
     8701 tacctaaaat tacggccttt tgtaaacgat tggctgatag aggcgtgtca tttaaacgat
     8761 taccgattaa gatagaggag ttcaaggagt cgctaaatgg atagtatgat tttagggcgt
     8821 tatatcccag gggattcgat tgttcaccga ttggatccac gtagcaaatt gcttgctatg
     8881 atgctactga ttttgattgt gttttgggct aataacccct tgacgaatct gattcttttt
     8941 atagcgacag ggatatttat tgccttgtca ggagtatctc tttcattttt tattcagggc
     9001 ttgaagtcta tgtttttctt gattgccttc acaactattt ttcaactatt tttcatttct
     9061 aatgggaatg ttttatttga gttttcgttt gtgagaatca cggattatgc tttgcaacaa
     9121 gctgggatta ttttttgtcg ctttgtattg attattttct tttcaacttt gttaacctta
     9181 acaaccatgc ctttaagttt ggcatcagct gtcgaagctt tattagcacc tttaaagcgt
     9241 gtgaaagttc cagttcatga aattggattg atgctgtcta tgagtttgcg ttttgtccca
     9301 accttgatgg atgatacgac tcggattatg aatgcacaga aagctcgtgg agtggatttt
     9361 ggagaaggaa gcatcgttca aaaagtaaag gcgatgattc ccattttgat tcctcttttt
     9421 gcgacaagtt taaaacgtgc agattccttg gctatcgcta tggaagcgcg tggctatcag
     9481 ggtggaaaag gcagaagtca ataccgacaa ttgaaatgga ctctaaagga tacgctgacc
     9541 attcttgtta ttctcgtact tggttgttgt ttatttttct taaaatctta gtagctttca
     9601 ggaattgata aaaagttact gtaacagttt ttgatataaa ggtagggata tgaaccgttt
     9661 taaaaaatca aaatatgtca ttattgtttt tgtcactgtt ctgcttgtgt cagctctctt
     9721 agcgacgact tattcaagta caattgtgac aaaattagga gatggaatct cattggttga
     9781 tagagttgta caaaaacctt ttcagtggtt tgattctgtc aaatcagatt tggctcattt
     9841 gacacgaaca tataatgaaa atgaaagttt gaagaaacag ctttaccaat tagaagttaa
     9901 atcaaatgag gtggaaagtt taaagacaga aaatgaacaa ctgcgccaat tgcttgatat
     9961 gaagtctaaa ttgcaagcca caaagacttt agcagcagat gttattatgc gttctccggt
    10021 atcttggaag caggagttga ccttagatgc aggtagatca aaaggtgctt ctgagaacat
    10081 gttagctatt gcaaatggtg gcttgattgg gagtgtttca aaagtagagg agaactctac
    10141 tatagtcaac cttctgacaa atacggaaaa tgctgataag atttctgtta aaattcaaca
    10201 tggctctact acaatttatg gaattattat tggctatgac aaggaaaatg acgttcttaa
    10261 aattagccaa ttaaatagta atagcgatat tagtgcggga gataaggtga ctactggtgg
    10321 attaggaaac tttaacgttg ctgatattcc tgttggtgaa gtggttgcca caacgcatag
    10381 tacagactat ttgacacgag aagtaactgt taaattgagt gcagatactc ataatgtaga
    10441 tgtgatagaa ttagtgggga attcataatg agacagttga agcgagttgg agtattttta
    10501 ttgcttcctt tctttgttct aattgacgcc catattagcc agcttctggg ctcatttttc
    10561 ccccatgtac atttggctag tcattttctt tttctatttc tcttatttga gacgatagaa
    10621 gtatcagagt atctctacct agtctattgt tttgttatag gcttggttta tgatgtttac
    10681 tttttccatc taatagggat tacaactctc ttatttatct tattgggagc cttccttcat
    10741 aaattgaata gtgttatttt gttgaatcgt tggacaagaa tgctagctat gattgtgctg
    10801 acattcctgt ttgaaatggg tagttatctt ttggctttta tggtagggtt gacagtagat
    10861 agcatgtcga tttttatagt ctatagcttg gtaccgacga tgattttaaa ttttttatgg
    10921 attactgttt ttcaatttat ttttgaaaaa tattatct
//
LOCUS       DAVLSL010000062        10930 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLSL010000062 DAVLSL010000000
VERSION     DAVLSL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.063"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(96..446)
                     /locus_tag="VXN30_001233"
     CDS             complement(96..446)
                     /locus_tag="VXN30_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357856.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(586..921)
                     /locus_tag="VXN30_001234"
     CDS             complement(586..921)
                     /locus_tag="VXN30_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357857.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1012..2322)
                     /gene="der"
                     /locus_tag="VXN30_001235"
     CDS             complement(1012..2322)
                     /gene="der"
                     /locus_tag="VXN30_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1357858.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2336..3049)
                     /locus_tag="VXN30_001236"
     CDS             complement(2336..3049)
                     /locus_tag="VXN30_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1357859.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3046..3942)
                     /gene="dnaI"
                     /locus_tag="VXN30_001237"
     CDS             complement(3046..3942)
                     /gene="dnaI"
                     /locus_tag="VXN30_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1357860.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3943..5112)
                     /locus_tag="VXN30_001238"
     CDS             complement(3943..5112)
                     /locus_tag="VXN30_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1357861.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQII
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(5113..5586)
                     /gene="nrdR"
                     /locus_tag="VXN30_001239"
     CDS             complement(5113..5586)
                     /gene="nrdR"
                     /locus_tag="VXN30_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1357862.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5726..6091
                     /locus_tag="VXN30_001240"
     CDS             5726..6091
                     /locus_tag="VXN30_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1357863.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            6096..6791
                     /locus_tag="VXN30_001241"
     CDS             6096..6791
                     /locus_tag="VXN30_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357864.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6808..7575
                     /locus_tag="VXN30_001242"
     CDS             6808..7575
                     /locus_tag="VXN30_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357865.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7987..9186)
                     /locus_tag="VXN30_001243"
     CDS             complement(7987..9186)
                     /locus_tag="VXN30_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1357866.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9183..10076)
                     /locus_tag="VXN30_001244"
     CDS             complement(9183..10076)
                     /locus_tag="VXN30_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357867.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQDQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10192..10272
                     /locus_tag="VXN30_001245"
                     /pseudo
     CDS             10192..10272
                     /locus_tag="VXN30_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10757..10930
                     /locus_tag="VXN30_001246"
                     /pseudo
     CDS             10757..10930
                     /locus_tag="VXN30_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3235 a         2390 c         1983 g         3322 t
ORIGIN      
        1 actatacaag atgaatattt ctctcaagtt tcataatcaa aataaaaatg actgtatcta
       61 tagctccata aacaaatcat agatacagtc attttttagt tcctacacct tcctttatgc
      121 acacctttag atttccctag ttcccgtctc aagtcaagca tttttttgaa acagatatag
      181 tctaaccagt cactagcaaa agcgataagt agtaataata gccaaatacc ccataacttg
      241 atatctgtca aatttctcaa gacgatattg aaaaacagaa ctgaaaaaac tgtccaagca
      301 aggctaaaaa gagcatagag ggtgatgtaa atccagtaaa aatagtaaaa aattgggaaa
      361 aatttacttt ctctattatc cttgtcaatc cagctgaaaa agtaaaaaca gggtaataag
      421 attatcattt taaacaagtg ttccatcaca agcctccttt tttaaattgt tttctttagt
      481 ttcgttctat taaatgttat taccaatata aaccttggaa tagcgtgtaa taaatgattt
      541 tagaggtacc ataagccacc tcctacaaat agaaaccgat ataaatcaat gccttccacc
      601 cttagacttc cctagttcct gtctcaagcg aaacatttct ttgaaacagg aataagttaa
      661 ccaattcata ccaatagcta gcagaataaa aagaaaccaa atgccccata acttgatatc
      721 tgtcacattt ctcaagacgg tattgaaaaa cagaactgaa acaactgtcc aagcaaggct
      781 aaaaagagaa tagaagggga tgtaaaacca gtaaaaataa taaaaaattg gaaaaaactt
      841 actatttctg ttggccttct caatccagtt atcaaaataa aagtacggtg ctaaaagtaa
      901 gaatttaaac aaatgttcca tcaccgacat caccctcctt cttttgatag cgttttctat
      961 tattttatta tatcaaaaaa atccggaact gtcattccag attctacttt tttatttgcg
     1021 ttttcttgcg atgagatgaa tcggtgttcc ctcaaaaaca aaggccttgc ggatttgatt
     1081 ttccaagaaa cgcaggtaag aaaagtgcat gagttcttct tcattgacaa agatgacaaa
     1141 ggttggtggt ttggttgcca cttgggtcgc atagaaaatc ttgagacgtt ttcctttgtc
     1201 tgtcggtgtt gggttgatgg caatggcatc catgatgaca tcgttcaaga cagctgatgg
     1261 aatacgtgta ttttgacttt cgctgatttg cttaatcatc tcaggaagtt tgtggagacg
     1321 ttgcttggtt aaagctgata caaagataat cggtgcgtaa ggcaggtatt ggaactgctc
     1381 acggatatct tcttcccagt ttttcatagt gtggttatct ttttcaagcg tatcccactt
     1441 gttgaccacg ataatcatcc ctttaccagc ttcatgggca aatcctgcga tacgcttgtc
     1501 gtactcacga atgccttctt ccgcattgat gaccatcaag accacatctg aacggtcaat
     1561 agcacgcatg gcacgcataa cagagtattt ctcagtattt tcataaacct taccagactt
     1621 acgcatacca gccgtatcaa tcatggtaaa ctcttgacca tctgtatctg taaagtgggt
     1681 atcaatggca tcacgagttg ttccagcaac aggactagca ataacacggt cttcgcccaa
     1741 gatagcattg atcaagcttg attttccaac gtttggacga ccaatcaggc taaacttaat
     1801 gacatctgga ttttcttctt catattcatt tggaaggttt tccacaatcg catctagtac
     1861 atcccctgta ccgataccat ggacagatga gataggcagt ggttcaccca aaccgagggc
     1921 atagaaatca tagatatcat ttcgcatctc agggttgtcc accttgttaa ctgcgaggat
     1981 aactggtttg tgggtcttat aaagcttacg ggctacgtat tcgtctgcat cggtaattcc
     2041 ttccttaccg gacacgacaa agacgataac atctgcttct tccatggcaa tttctgcctg
     2101 gtgcttgatt tgttccatga aaggagcatc gacatcatca attcctcctg tatcaatcat
     2161 gctaaaagaa cgattgagcc actcacccgt tgcataaata cggtcacgtg tcactccttc
     2221 gacatcttct acaatggaga ttcgctcacc agcgatccga ttaaataggg ttgatttccc
     2281 aacattggga cgtcctacaa tggcaatagt tggtagggcc ataatttctc actttctaca
     2341 ataatttctt ctgttcaaga ttttttctag ttgagcttgg ttcagcttga ccaaactgtt
     2401 ctgctaggcg ctgactccag cttgtggtcg cacgcgcccc agcatagtca gcctgaacac
     2461 ggtcataagc ttggattgcc tcagttgact gttcttggta ttcttcctca aagacaacat
     2521 tctctagtgg cagtctcggt ttcatatcat gatgttgatt tggcacaccc agtgccatcc
     2581 caaagacaga ataggtgtag tcaggtaggt taaagagctc tgccacttct tcagacttgt
     2641 atcgaaccaa accgataatc acaccaccat agcccaagct ttcagctgcc aacaaggcgt
     2701 tttgtccagc aagagctgca tcgaccgaac taatcaagag accttccaca ccttggggtt
     2761 ggaaggtgtc ggtatgaagt cgggctccct tttctgctcg gttcaaatct ccgacaaaga
     2821 gaaggaaaac agcagactgg cgaatggctt cttgaggtac caattcatac aaggcatctt
     2881 tcttctcttg acttcgtacc acaatcacag agtaggattg gaaattcttc caagatgatg
     2941 ccatctgggc tgctgtcaaa atctcattta agtctacttg gggaatttct tgctctttaa
     3001 acctgcgcac tgaagtatga gccttcatca atttaatggt ttctgtcatc gacggtttac
     3061 tccttctaaa cgagtctcct cagccaaata acggatgcgt tccatgaccc gtctggcttc
     3121 ccaggtttcg tcatttccat gtttcacttt cgcaaaatgc ttctccaaat cttcaaagtt
     3181 gaagttggat gtgaaaaagg tcggtaaatt ttcctgcatc cgatattgga gaatgacctg
     3241 caggatttcg tcacgcaccc aagcggtgga ttgctcggca ccaatatcat ctaaaatcag
     3301 gacttcagaa agcttaatct catccactaa ggtcttaaca ttgccatcac cgatagcatt
     3361 tttgacatca atgacaaagc taggatagtg gaggagagtt gatgaaacac cacgtttttc
     3421 tgataaatca tgagctaagg ccgccaccat gaaactttta cccacaccaa agtctccata
     3481 taagtaaaga ccttttcgaa tagctggata ttgctccacg aaggctagta gcttttcaaa
     3541 aactggtaag cgccccaaat catccaagtc aacttgagcc aaactagctt tcttgagact
     3601 ggctggtaga ttgattaact tgagacggtt cttaatagcc gcttcttttt cagccgcgat
     3661 tagctcagga gtttcttcat atgaaacatc tgcatagcca tgattcttaa ccaaaatcgg
     3721 cttgtagcct ttggcaatat aatccgtatc cccacggaga aacttgtcac gctcggtgat
     3781 gtactgatta aacttggaga tactgcgatt taattcctct ggagtcaagg attcttgctg
     3841 gataaaagcc gcaacatcag ggtccttcat gattttctgg accaaatctt gataataaaa
     3901 acggctgggt tgacgtttga gtacgtctcc gacactttcc atctaatctc ctcctttttc
     3961 taatcgagct aatagttctt gcttcttacg ttctagttcc agacgagttt cctcgctggt
     4021 ttcattctta tattcaggat tactccattt aggaacattg gtttttactg gggcagtctg
     4081 attctgtttt tgtgtttttg ctttctgtcc tcgctcacgg atgcgcaaga ctgccttttc
     4141 tgccgaatga atcttttgat aggcatagtc attggctacc ttcatggcat atttctcatt
     4201 gatatttgcc gaatcgacct tattaaaggt caataaaaga atgatattga tgacttcgtc
     4261 cagcaagccc aagccagcca tctgttgcaa gagttctctt tctgtttggg taatggttcc
     4321 cttgcgtgtt tgcttgattt ctgccaagaa ctgcagggca gttttacttt tggcttcttt
     4381 gataatggtc gcttccttaa gactaaagtc agaggaaact ggtttttgag caattttttc
     4441 acgcatgcgt ttggttgaaa taatctgaga aacagctgtt gacttggcca attgataggt
     4501 ttcaaaccaa gtccatttct tctcctcggc aatagcaaag aggtttaaga catcggactg
     4561 ctcatccgca aagcgaagtc catctcgagc cataagctga cgaaaatggt ccaagtcaaa
     4621 atcgttggcc actttcttct tgagaccaag gtcttcttga ctgcctagtt ctgccaattc
     4681 tggaaagact tgattgagtg agacaggtat ttcttcacca tcagcacttt caactttcaa
     4741 atcctccaca gctacatcgc caatcttttt ctctaagagt ctgcgataaa caggatgccc
     4801 caagaagtct tgactagata gaggagcatg gagggctagc tgataaacat cccccttttg
     4861 atagagagtc aagagattaa aagcagataa gattttcaat gattttatca gtctatccat
     4921 cccaaagttg agatgattga ggatacttga aaaaagatat tcctttctac cattatccca
     4981 aaaactaatc gtataaagat aaaggctcag tgcctcctga ccgataatcg ggaggtagca
     5041 ctgtaccaga gatgaggtat cttgcgacac ccgattattc tttagataag aaaaacggtc
     5101 aattggcttc atttatcttt cctttttctt tttagaggac tgggtgattt gttggagcaa
     5161 gctctctaac tcactgacat ccttaaaact acgatagaca ctagcaaaac gtacataggt
     5221 aatctcgtcc aattcagcca actcctccat gacgagtgaa ccaatgtcct cactttgaat
     5281 ttcattttca tttcgaccac ggagtttctg ttcgatacga ttgactacca tgttgatttc
     5341 atcacttgac acaggacgtt tctgggctga gcggataatc ccattaaaga ttttatctct
     5401 ggagaattgt tcccgtgtgc catctttttt aacaaccact aaggttcttt cttctactcg
     5461 ttcgtaggtt gtaaaacggt gttggcattc gtcgcactca cgtcttctac gaatggtgtt
     5521 cccttcttct gcttggcgac tatcgataac acttgacttg gtagccccac attttggaca
     5581 acgcatcctt tccctcctta tcgttttctt ttcattatac cattttttaa gcgattccca
     5641 aaacaattct tctttttgct tgacaagttt tttgttttgt tgtattattt agttaagaca
     5701 aaaggataag agaaaggaga ctaagatgtc ctggacattt gacaacaaaa aacccatcta
     5761 tttacagatt atggagaaaa tcaagcttca gattgtttcc catacactgg aacccaatca
     5821 acaacttcca accgtgaggg agctagctag cgaggctggt gtcaatccca ataccatcca
     5881 aagagcctta tcagaccttg aacgagaagg atttgtctac agcaagcgaa caactggacg
     5941 atttgtgact aaggataagg agctaatcgc tcagtcacgc aaacaattat cagaagaaga
     6001 attggaacac ttcgtttcct ccatgatcca ttttggctat gaaaaagaag aactaccagg
     6061 cgtagtcagt gattatatta aaggagttta agcctatgtc attactagta tttgaaaatg
     6121 tatccaaatc atatggagca acaccagccc ttgaaaatgt ttctcttgac attccagctg
     6181 gaaaaattgt cggccttctt gggccaaacg gctcaggaaa aacaaccctg attaaactaa
     6241 ttaatggcct cttacaacca gatcaaggac gtgtcctcat caacgacatg gacccaagcc
     6301 cagcaaccaa ggccgttgta gcttatttgc ctgatacgac ctatctcaat gagcaaatga
     6361 aggtcaaaga agccctaacc tacttcaaga ccttctataa agatttcaat cttgaacgcg
     6421 ctcatcatct acttgcagac ctgggcattg atgaaaatag tcgtctcaag aaactatcaa
     6481 aaggaaacaa agaaaaggtt caactgattt tggttatgag ccgtgatgct cgtctctatg
     6541 ttttggatga acccattggt ggggtggatc cagcagcccg tgcttatatc ctcaatacca
     6601 ttatcaacaa ctactcacca acttctaccg ttttgatttc tacccacttg atttctgata
     6661 tcgagccaat cttggatgaa attgtcttcc taaaagacgg aaaagtcgtc cgtcaaggaa
     6721 atgtagatga tattcgctac gagtcaggtg aatccattga ccaactcttc cgtcaggaat
     6781 ttaaggccta agcaaaggag attatttatg ttttggaatt tagttcacta cgaatttaaa
     6841 aatgttaaca agtggtattt agccctctac gcagccgtgc tagtcctttc tgccctcatc
     6901 ggaatacaga cacaaggctt taaaaatcta ccttaccaag aaagtcaggc tactatgcta
     6961 ctttttctag ctacagtctt tggtggcttg atgcttacac ttgcgatttc aaccattttc
     7021 ttgattatta aacgcttcaa aggtagtgtc tacgaccgac aaggctatct gactttgacc
     7081 ttgccagttt ctgaacacca tatcatcaca gccaaactaa tcggtgcctt tatctggtca
     7141 ttgattagca ccgctgtatt ggctctaagt gctgttatta ttctggcttt aacagctcca
     7201 gaatggattc ctctttctta tgtgattaca tttgtagaaa cacatctccc tcagatcttt
     7261 cttacaggta tatccttcct actaaatacc atttcaggaa tcctctgcat ctacctggct
     7321 atttccattg gacagctttt caatgaatac cgtacagcac tcgctgttgc agcctacatt
     7381 ggtatccaaa tcgtcattgg atttattgaa cttttcttca atcttagttc taatttctat
     7441 gtcaattcac tggtaggact caatgaccat ttctatatgg gagcaggtat agccattgtt
     7501 aaagaactca tattcatagc tatcttttat ctcggaacct actacatctt gagaaataag
     7561 gttaatttgc tttaaataat ttttacctag atatgtaaca tactcataga acaaaagaga
     7621 ccaggcaaaa agtctttaaa attagaaaac gcatagtatc aagtgttgaa tatgaactgc
     7681 ccccaaaagt tagacagaaa aaatctaact tttggggggc agttcaaaaa atccttgata
     7741 ttatgcgttt tttgtgagct gacttatttc ctttcactat atcgcaaaat gaaataagaa
     7801 cggaacgatg ggattttgga attcaaatca atttataaga atgttttaga agtaatatta
     7861 tcctattcca gattcagttc actatacaat tgagttttca agcaacctgt ttacataatg
     7921 tgtacataat taggttcgtg attccaccct tttcaccttt aaaaacctcg ctttcgcaag
     7981 gctcttctat ttataagata aggcacgttt aaaggttttc caaatcccta aatcatccgt
     8041 ttgaagaacg agactagcat acatgcgtcc gataaatcct gttgctacca ccgcaaaaat
     8101 cactgtaata gcaagtgaaa tccatgcttc tgctcccccc gcatagccat taatcgttcg
     8161 aaacggcata aagaaggtcg aaataaaggg aatataagaa ccaatcttca agaggagatt
     8221 gtcaccagct gcacctagag ctgtcactcc aaaaaaacca cccataatca aaatcatcaa
     8281 aggcgacaag gctttccctg agtcctcagg acgagaaacc atagatccta ggaaggctgc
     8341 caagactaca tacatgaaaa gactgatcaa aataaagagc aaggtattca gtgagatagc
     8401 atctcccaag tgatccaaaa taccagactg agccaagaat ggcaaatctt taaagagcaa
     8461 aacggcagcc agaccaccta caacatagat cccaatatgc gttaaaatca ctagaaacag
     8521 agccatcatc cgcgcataga aatagtgact tgcccttatg ctagaaaaaa cgacttccat
     8581 aattttggtg cctttttcac tggcaacttc ctgagccgtt acacccgcat aggtaatcag
     8641 aatcatataa agaaagaatc ctaaggcacc tgctgcaatt gtttgaataa actttttatt
     8701 ttccttggct tcatcaatct tttctgtgaa ttgaattgtc tgcgctaagc gtttttcctg
     8761 ctcttgagac aaggaagcag ttgaacgatt aagctgattt tgcagttcat tgagtgtacc
     8821 tgtaacctca aatttaattc cattttcaag cgatgtttcg ccatgataaa ctgcctttag
     8881 aacactatct tcttgatcaa tggtcaaata accttttaat ttttcttctt taattgcttc
     8941 tttggcactt gcttcatctt tatagtcgaa gttaacacca tttacattct tcagtccttc
     9001 tgctacagat ggcactgttg tcactactgc cactttatta tttttagcca tagaagaacc
     9061 ttggagatgc ccaattccta cagagattcc taaaaagagg aacggcgaaa tcaccataaa
     9121 gaagaaactc catgactcga catgtcgaag ataggtttcc ttgattacaa cccacatatt
     9181 tctcatactt ccactcctga ttctagttta aagatttcat cgatagttgg cgcttgttgg
     9241 tcaaatgttg cgatatattg atcttgagtc aagattggga agagttccct tccagcgctc
     9301 tcatcctcca aaatcaattt ccaactgcct tgtttggtca agctcacctg tttgacatga
     9361 ggaagatttt ccaattcttc cttgcttcgt tcacttgaaa caaagagacg cgttttcccg
     9421 tattgattgc ggacatcctg aactggtccg tgcaagacca cacggccatc tcggatcatc
     9481 agaatatcgt cacaaagttc ctcaacattg gtcatgacat ggtcagaaaa gataatggtt
     9541 gctccgcgct ctttttcctg aaaaatgact tgtttgagca attctgtatt aactgggtcc
     9601 aatccactaa aaggctcatc caagataatc aggtctggtt catgaatcag agtaataatg
     9661 agctgaatct tctgctgatt tccttttgac agactcttga ttttatctgt cagctttcct
     9721 ttcacttcca acctcttcat ccattgaggg agtttttctt tgacttcttt ggcatccatg
     9781 ccttttagag tcgccaagta gcgaacttgt tcaagaactg tcaatttagg catgagactg
     9841 cgttcttcag gcagataacc aatccgagca taggtctcct gacgaatatc ctgaccatcc
     9901 agaccgattt ctccctgata ttctaggaat tttaaaatac tatggaaaat cgttgttttt
     9961 ccagcaccat tttttccgac tagtcccaaa atacgacctg gtcgcgcttg aaagtcaata
    10021 ccaaacaaaa cttgcttgga tccaaaactt ttctctagac ttcttacttc tagcatcttt
    10081 cacctccgaa atttcttgca ctcattatac tcctttttga tagcctttac aatgtttttg
    10141 tccattttta gaagactatt gctgtgtaaa atatggcctg gagcactttt atactcaatg
    10201 aaaagcaaag agcaaactag gaagctagcc gtaggctgct caaagtacag ctttgaggtt
    10261 gcagataaaa ctgacgaagt cagctcaaaa cactgttttg aggttgtgga tagaactgac
    10321 gaagtcagta actatatcta cggcaaggcg aagctgacgt ggtttgaaga gattttcgaa
    10381 gagtattagt gataaatcca ttatacagta gcaaacttaa tttatacctt ccgctcctca
    10441 actgtctatt tttaatcctg aattgttatt tgagtaactc ctttttcctc ataaagtttt
    10501 cttcctctaa aacttctgga aaaaagcgaa tagtttcaga caacattttt ataagaaaca
    10561 agttcatctg tcatttcaag aaggagtaat cctttatcta ctaatggacg gaacggaatt
    10621 caaccgcttg tccgatatgt tttctaagga ttatatagta aaatgaaata aaaacaggac
    10681 aaatagatcg ggacagtcaa atcgatttct aacaatgttt tagaagtaga tgcgtactat
    10741 tctagtttca atctattata tctattatgc acaccactat aggatctaat aaaaatcaca
    10801 acaagttcat tcatagatgg ttacctcagg gaactaagaa aacgactaca aaggaagtcg
    10861 cattcatcga aaagtggatt aacaactatc ctaaaaaatg cttgaactac aagtccccca
    10921 gagaagactt
//
LOCUS       DAVLSL010000063        10778 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLSL010000063 DAVLSL010000000
VERSION     DAVLSL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.064"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            85..582
                     /locus_tag="VXN30_001247"
     CDS             85..582
                     /locus_tag="VXN30_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1357868.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            582..2237
                     /gene="dnaX"
                     /locus_tag="VXN30_001248"
     CDS             582..2237
                     /gene="dnaX"
                     /locus_tag="VXN30_001248"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1357869.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2265..2459
                     /locus_tag="VXN30_001249"
     CDS             2265..2459
                     /locus_tag="VXN30_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1357870.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2609..3379
                     /gene="sufC"
                     /locus_tag="VXN30_001250"
     CDS             2609..3379
                     /gene="sufC"
                     /locus_tag="VXN30_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1357871.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3407..4669
                     /gene="sufD"
                     /locus_tag="VXN30_001251"
     CDS             3407..4669
                     /gene="sufD"
                     /locus_tag="VXN30_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1357872.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGDDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4680..5906
                     /locus_tag="VXN30_001252"
     CDS             4680..5906
                     /locus_tag="VXN30_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1357873.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5893..6333
                     /locus_tag="VXN30_001253"
     CDS             5893..6333
                     /locus_tag="VXN30_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1357874.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6387..7799
                     /gene="sufB"
                     /locus_tag="VXN30_001254"
     CDS             6387..7799
                     /gene="sufB"
                     /locus_tag="VXN30_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1357875.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8030..9271)
                     /locus_tag="VXN30_001255"
     CDS             complement(8030..9271)
                     /locus_tag="VXN30_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1357876.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKNLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9374..10483
                     /locus_tag="VXN30_001256"
     CDS             9374..10483
                     /locus_tag="VXN30_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1357877.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3194 a         1966 c         2341 g         3277 t
ORIGIN      
        1 aaatattata gagcccaaaa tcctttgttt actaaacaag ggatttttct tttgtctctg
       61 ctcctttttt gatataatag ttctatgtta aaatcagaaa aacaatcacg ttatcaaatg
      121 ttaaatgaag aattgtcctt cctattggaa ggcgaaacca atgttttggc taatctttcc
      181 aacgccagtg ctctcataaa atcacgtttt cctaataccg tatttgcagg cttttatttg
      241 ttcgatggaa aggaattggt tttaggcccc ttccaaggag gtgtttcctg catccgtatt
      301 gcactaggca agggtgtttg tggtgaggca gctcactttc aggaaactgt tattgttgga
      361 gatgtgacga cctatctcaa ctatatttct tgtgatagtc tagctaaaag tgaaattgtg
      421 gtgccgatga tgaagaatgg tcagttactt ggagttctgg atctggattc ttcagagatt
      481 gaggattacg atgctatgga tcgagattat ttggaacaat ttgtcgctat tttgcttgaa
      541 aagacagcat gggactttac gatgtttgag gaaaaatctt aatgtatcaa gcactttatc
      601 gaaaatatag aagtcaaaac ttctcccagt tagttggtca agaagttgtg gctaagactc
      661 ttaaacaagc ggtggagcaa gagaaaataa gtcacgctta tcttttttct ggtcctcgtg
      721 gaacgggaaa aaccagtgtt gctaaaatct ttgccaaggc tatgaactgt cccaatcaag
      781 tgggtggcga accttgcaat aactgctata tttgtcaagc agtgacggac ggtagtttag
      841 aagatgtcat tgaaatggat gcagcttcta ataatggggt agatgaaatt cgcgaaattc
      901 gtgataaatc tacctatgcg cctagccttg ctcgttataa ggtttatatc atagatgagg
      961 ttcacatgct gtctacaggg gcttttaatg ccctcctaaa gacgctggaa gaaccaacac
     1021 agaatgtagt ctttattttg gccactactg aattgcacaa gattcctgct actattctat
     1081 cccgtgtgca acgttttgag tttaaatcaa ttaagacaca ggatattaag gaacatattc
     1141 actatatctt agaaaaagaa aatatcagtt ctgaaccaga ggctgtggaa atcattgcca
     1201 gacgggcgga aggtggaatg cgggacgcct tgtctatttt ggatcaagcc ctgagtttga
     1261 cacagggaaa tgagctgacg actgctatct ctgaagaaat tactggcacc attagcctat
     1321 cagccttgga tgattatgtg gcggccttgt ctcaacagga tgttcccaaa gctttgtctt
     1381 gcttgaatct tctttttgac aatggtaaga gcatgactcg ttttgtgacc gatcttttgc
     1441 actatttaag agacttgtta attgttcaaa cagggggaga aaatactcat catagttcag
     1501 tctttgtaga aaatttggca cttcctcaaa aaaatctgtt tgaaatgatt cgcttagcaa
     1561 cagtgaattt agcagatatt aagtctagtt tgcagcccaa gatttatgct gaaatgatga
     1621 ccgtccgttt ggcggaaatc aagcccgaac cagctctatc aggagcggtt gaaaatgaaa
     1681 ttgctacgct gagacaggaa gttgcccgtc tcaaacaaga gctttctaat gcaggtgcgg
     1741 ttcctaaaca agttgcacca gctcctagtc gaccagctac gggcaaaaca gtctatcgtg
     1801 tcgatcgcaa taaagtgcaa tctatcttac aagaggccgt cgaaaatcct gatttagcac
     1861 gtcaaaatct aattcgtttg cagaatgcct ggggagaggt aattgaaagt ctaggtgggc
     1921 cggacaaggc tctgctagtt ggttctcaac cggttgctgc caatgaacac catgctattc
     1981 ttgcttttga gtctaacttc aatgctggtc aaactatgaa acgagacaat ctcaatacca
     2041 tgtttggtaa tatcctcagt caggcggcag gtttttcacc tgagatttta gctatttcca
     2101 tggaggaatg gaaagaagtt cgcgcagcct tttcagccaa agccaaatct tctcaaactg
     2161 aaaaagaagt agaagaaagc ctgattccag aaggatttga atttttggct gataaagtga
     2221 aggtagagga agactaaaga aagatttcat gatacaataa gtttatgaat aaacaacaat
     2281 ttattattat ggcgctattt acagctgctg agacctattt tttcaatgaa gcctggatga
     2341 ctggccgcta tattatggca gccttttggg caattttact ctttagaaat ttccgagtca
     2401 gttatgtgat gggcaaaatc gttgatgtca tcgatcagca ttttaatagg aaagactagc
     2461 cctcagcttc cagacaaaat caaagccttt taggcttttt tttgttatac tagaaaagta
     2521 tatttataga atttttgctc tatttttggg gaaatcagac gtttttctag taagtactgt
     2581 aaaagttttg aaaaagaaag gaactatcat gtcagtatta gagatcaaag atcttcatgt
     2641 tgagattgaa ggaaaagaaa ttttaaaagg ggttaacctg accctgaaaa caggagaaat
     2701 tgccgctatc atgggaccaa atggtacagg taaatcgact ctttctgccg ctatcatggg
     2761 aaatccaaac tatgaagtaa ctaaaggtga agttttgttt gatggcgtaa acatccttga
     2821 gttggaagtg gatgagcgtg cgcgtatggg acttttcctt gctatgcaat acccatcaga
     2881 aatccctgga attaccaatg ctgagtttct tcgtgccgct atgaatgcgg gtaaagaaga
     2941 tgatgagaag atttcagttc gtgagtttat tactaagcta gatgaaaaaa tggaattgct
     3001 caacatgaaa gaagaaatgg cagagcgtta cctcaacgaa ggcttctctg gtggtgagaa
     3061 aaaacgcaat gaaattcttc aacttttgat gttggagcca acatttgctc ttttggatga
     3121 gattgactca ggtcttgata ttgacgctct taaagttgtg tctaaaggtg tcaatgccat
     3181 gcgtggtgaa ggttttggtg ctatgatcat tactcactac caacgtcttt tgaactatat
     3241 cacacctgat gtggtacacg tgatgatgga aggtcgtgtt gtcctttctg gtggtccaga
     3301 attggctgcg cgtttggaac gtgaaggata cgcaaaacta gctgaagaac ttggctacga
     3361 ctacaaggaa gaattgtaat tccctcgtat cttttaggag aagtaaatga ctagagaaaa
     3421 tattaaactt ttttcagaaa tgcacgctga accaagctgg ttggctgatc tccgtcaaaa
     3481 agcttttgac aagattgaga ctttggaatt accagttatt gagcgtgtca aattccaccg
     3541 ttggaatctg ggtgatggaa cgattacaga aaatgagcca tcagcaaatg ttccagattt
     3601 cacagcttta gatcatcact tgaagttggt gcaagtagga actcaaactg ttttcgaaca
     3661 aactccagtt gagttagctg aacagggtgt tgtcttcaca gactttcact cagctttaga
     3721 agaaattcca gagctgatcg aagaattctt catgtcatct gttaagtatg atgatgacaa
     3781 gttggcggct taccacacag cttactttaa cagtggtgct gtactctata ttccagataa
     3841 cgtagaaatt acagagccaa ttgaaggaat tttctaccaa gatagcgata gcaatgtgcc
     3901 gtttaacaag catattatga ttatcgctgg taaaaattct aagattagtt atctggagcg
     3961 tttagagtca cgcggtgaag gaagtgacaa agcaactgcc aatatcacag tggaagtgat
     4021 tgcacgttct ggtgcgcaag tcaagtttgc tgctatcgac cgtctaggtg aaaacgtcac
     4081 tgcctacatt agccgtcgtg gtaaattagg cgacgatgca agtattgact gggctatcgg
     4141 tgtcatgaac gaaggaaatg tcgttgctga ttttgatagt gacttgattg gtaatggtag
     4201 ccatgctgac ctgaaggttg tagctctttc aagtggtcgt caggtacaag ggattgatac
     4261 tcgtgtaact aactatggct gcaactctat cggaaatatc ctacaacatg gggttatcct
     4321 tgaaaaagca actttgactt tcaatggtat cggccacatc atcaagggtg ctaagggagc
     4381 agatgcgcaa caagagagcc gtgttcttat gctttcagac caagcgcgtt cagatgctaa
     4441 cccaattctt ttgattgatg aaaatgacgt aactgcagga catgcagcct ctattggtca
     4501 ggtagatcca gaagacatgt attacctcat gagtcgtggc ttggataagg caactgcaga
     4561 gcgtttggtt gttcgtggtt tccttggatc tgttatcgtg gagattccag tcaaggaagt
     4621 tcgtgatgaa atgattgcaa ctatcgaaga gaaattgtca aaacgctaag gggaagccta
     4681 tgttagatgt agaagcgatt cgcaaggatt ttccaatttt agatcagatt gtcaatgatg
     4741 aacctctggt ctatctggac aatgctgcga cgacacaaaa accactagta gttctgaaag
     4801 ctattaacag ctactatgag caggacaatg ccaatgttca ccgtggtgtc cataccttag
     4861 cggaacgagc gacagcttct tatgaagctg ctcgtgaaac cattcgtaag tttattaatg
     4921 caggctctac aaaggaagtt ctctttacca gaggaacgac aaccagcctt aactgggtgg
     4981 cacgctttgc tgaggaaatt ctcgctgagg gagaccaggt cttgatttca gtaatggaac
     5041 accattctaa tatcattcca tggcaggaag cttgtcgaaa gactggagca gagcttgtct
     5101 atgtctatct taaagacggt gccttggata tggaggattt gcgagctaaa ttgactgata
     5161 aggttaaatt tgtttcccta gctcatgcct ccaatgttct tggtgtggtc aatccgatca
     5221 aggaaatcac tcaattagcc caccaagttg gggcaattat ggtagtggat ggtgctcaat
     5281 ctacacctca tatgaagatt gatgtccagg acttggatct ggactttttc gccttttcgg
     5341 gtcacaagat ggctggtccg actggtatcg gtgtccttta cggcaaagaa aagtatcttg
     5401 agcaaatgtc tccagtagaa tttggcggcg agatgattga ttttgtctac gagcaatttg
     5461 ctagttggaa ggaattgcct tggaaatttg aggctggaac gccaaatatg gcaggagcta
     5521 ttggacttgc gactgcagtt gattatctgg aaaagattgg tatggatgcc gttgaagctc
     5581 atgaacagga attgattgcg tacgtctatc caaaactgca ggcaattgag ggattgacca
     5641 tttacggttc tcaggatttg gctcaacgtt cgggtgttat tgcctttaac ctaggtgatc
     5701 tccatcctca cgatcttgcg acggctctgg attatgaagg agtggctgtt cgtgctggtc
     5761 accattgtgc gcaacccttg cttcagtatt tggaagtccc agcaacagct cgtgcaagtt
     5821 tttatatcta caataccaag gcagattgcg acaaactagt cgatgcccta caaaagacaa
     5881 aggagttttt caatggcact ttctaaacta gatagccttt atatggcagt ggtagcagac
     5941 cattcgaaaa atccacatca ccaagggaag ttagaagatg ctgagcaaac cagtctcaac
     6001 aatccgactt gtggggatgt catcaacctc tctgtcaagt ttgatgcaga ggaccgtttg
     6061 gaagatattg cttttctaaa ttcaggatgc acgatttcaa ctgcttctgc tagtatgatg
     6121 acagatgccg ttttaggaaa aaccaaacaa gaaattttag aactggcgac tattttttct
     6181 gaaatggttc aggggcaaaa agatgagcgt caaggccaac ttggagacgc ggcattcttg
     6241 tcaggtgttg ccaaattccc tcaaagaatc aagtgtgcaa ccctagcttg gaatgccctt
     6301 aagaaaacaa ttgaaaatca agaaaaacag taagacaagt ttcttttgtc ttatgaatta
     6361 ttagaaatga agaaagaaag gatactatgg ctgaagaaag agtagaacca aaaccaattg
     6421 accttggtga atataaattt ggtttccatg acgatgtaga gcctgtctta tcgacaggaa
     6481 aaggactcaa cgaaggtgtt attcgtgaat tatctgctgc taagggtgag cctgagtgga
     6541 tgttggagtt ccgtttgaag tcttatgaaa ccttcaaaaa aatgcccatg caaacttggg
     6601 gagcagactt gtcagagatt gactttgatg acttaatcta ctaccaaaaa ccatctgaca
     6661 aaccagcccg ttcttgggat gatgtacctg aaaagattaa agaaaccttt gaacgtatcg
     6721 ggattccaga agctgaacgt gcttatttag caggggcttc tgcccagtac gagtcagaag
     6781 tggtttacca caacatgaag gaagagttcc aaaaattagg tattatcttt acagatacag
     6841 attccgcact caaggaatac ccagacttat ttaaacaata ctttgcgaag ttggtaccgc
     6901 cgacagataa caagttggca gccctcaact cagcagtatg gtcgggtgga acttttatct
     6961 acgtgccaaa aggtgtcaag gtagatattc cacttcaaac ttatttccgt atcaataacg
     7021 aaaatatagg tcagttcgaa cgtaccttga ttatcgttga tgagggagca agcgtccact
     7081 acgtagaagg atgtacagca ccaacatatt caagcaatag cttacacgct gccattgtag
     7141 aaatttttgc tttggacgga gcttatatgc gttatacaac tatccaaaac tggtctgata
     7201 acgtctataa cttggtaaca aagcgtgcta aggctcaaaa ggatgccact gttgagtgga
     7261 ttgatggaaa cttgggtgcc aaaacgacta tgaaatatcc atctgtttac cttgatggag
     7321 aaggagcgcg tggtaccatg ctctctatcg cctttgctaa tgcagggcaa caccaagaca
     7381 cgggtgctaa gatgattcac aatgctccac ataccagctc gtctattgtg tctaaatcca
     7441 tcgctaaagg tggaggaaag gttgactacc gtggacaagt cacctttaac aagaactcta
     7501 agaaatctgt ttcccacatt gaatgtgata ccattatcat ggatgacttg tcagcatcag
     7561 atactattcc atttaatgaa attcacaact cgcaagtggc tttggaacac gaagccaaag
     7621 tatctaagat ttcagaagag caattgtatt atctcatgag ccgtggattg tcagaatctg
     7681 aggcaactga aatgattgtc atgggatttg tagaaccctt tacaaaagaa cttccaatgg
     7741 aatacgcagt tgagctgaac cgcttgatta gctatgaaat ggagggatca gttggataaa
     7801 atttgatttt atactcttcg aaaatctctt caaaccacgt cagcatcgcc ttaccgtatg
     7861 tatggttact gacttcgtca gtttcatcta caacctcaaa acagtgtttt gagcaacctg
     7921 cggctagctt cctagtttat tctttgattt tgagtattag atttactcaa aatcaaggat
     7981 tttgaagatg aacttgtatc aaaaaatcgc ggtttaaaat cgcgattttt tataatttct
     8041 cgttaacaaa gcggacaaac tgattccacc aaacttttaa gaagaaggct ttttcaattt
     8101 tcttgtctgc taccatttcg aaactagggc gctctgtggt gatgtaacct tgaccaatca
     8161 agttcttgtc ttcataagtc aaatggccaa ccactgttcc agcttcaagt ggtgctggga
     8221 ttgctttgga atcaggtgtg aattgaacag attgggaaga ttgattccca acacgttcta
     8281 ttagatagat atcctctgga gccactgcag ttactgtatc ttcttttcca tcttgtacag
     8341 gggctttgct atcttgatag gcatcgcctt gttgaacgat tttgcgaagt gtaaatgtag
     8401 aagaaatata atccattagg gaagatgtag ctgtaaatcg agcgtaagga ttattgtctt
     8461 gatgatctgc atttaaaaca actgtgatga ctctcatgcc tttttcgaca gtagtaccaa
     8521 caaaagactc tccagcctta tctgttgttc ctgtttttag cccatcaaaa ccaccacggt
     8581 aagcaggcat accttctaac atgtagttgg ttgaagtgat tgtcatccca gcaaaagtag
     8641 aagaaggttt tttggtgatt tctaagactt gtgggtattt tttgatgagg ttgcgagcaa
     8701 cgatagcgac atcataagca ctaagcttat tttcctcatc ttttttagaa cctgggtaaa
     8761 tgttatcccc tagagtttca ttgttaagac ctgtcgtatt gacaacagtg gcatcctgaa
     8821 ttccccattc caagagtttt gcccgcatca tatcgacgaa atctttttct gagccagcaa
     8881 ttttctcagc tagggcaata gcggcgctgt tggcactaga taccagagtt gcttcaagca
     8941 actcttcgac agtataatta cgggcctcca taggaatatt actggcttca gaatttgtcg
     9001 tcaattgata aggataatca gaaatatcta caggagtgga gagggtaata cttccgtttt
     9061 ccaaagcttc atagaccaga taaacagtaa tcaattttgt tatggaagca atttcgacag
     9121 gttgcgttgc atccttctca tagagaattt taccagtatt tgcctcaaca gcaatcgcat
     9181 gtttagcggc aatggtaaaa tcttgagcaa cagcagtaga agcaccccct aaaagagaga
     9241 cagttaacaa agttaaaaat atttttttca tagtagtttt attctatcat aaagaaaaaa
     9301 aatattcttg ctttaataat tcatctgtta agctttttga aaatatggta aaataaagtg
     9361 agggaggtaa ctcatgtttc gtagaaataa attatttttt tggaccacag aaattttact
     9421 cttaaccatc atcttttacc tatggagaca gatggggtct ttgattaacc cttttgttag
     9481 cgtgcttaat acaattatga ttccattttt attagggggc tttctttatt atttgacaaa
     9541 ccctattgtt actttcttaa ataaagtctg taaactcaat cgtttgcttg gtattttaat
     9601 taccttgtgt actttggtct ggggaatggt cataggtgtt gtctatctct tacctatttt
     9661 gattaatcag ttatctagtt tgattatatc tagtcaaact atttatagtc gagtacaaga
     9721 cttaatcata gacttatcta attatcctgc gctccagaat ttggatgtag aagctacaat
     9781 tcagcagtta aacttatcct atgttgatat tcttcaaaat atcctaaata gcgtatcaaa
     9841 tagtgtgggg agcgtcttgt cagctcttat cagtactgtt ttgattttga ttatgactcc
     9901 agtttttttg gtttatttct tattagatgg acataaattc ttgcccatgc ttgaaagaac
     9961 gattctaaag agggatcgct tgcatattgc aggcttatta aagaatttaa atgcgacgat
    10021 tgctcgctat attagtggag tttcgattga cgcaatcatt ataggttgtt tagcttatat
    10081 tggctatagt attattggtt taaaatatgc tttagttttt gccatttttt ctggtgtagc
    10141 caatttaatt ccttatgtgg ggccaagtat tggtttgatt cctatgatca tcgcaaatat
    10201 attcactgat ccccatagac tgctgattgc agtgatttat atgcttgttg ttcagcaggt
    10261 agatggcaat atcttatatc ctcgaatcgt aggaagtgtt atgaaggttc atccaatcac
    10321 gattttagtt ttacttttgt tgtcaagcaa tatctatggt gtagttggaa tgattgtcgc
    10381 agtgccaacc tattctatct tgaaagaaat ttctaagttc ttatcccgtt tgtatgaaaa
    10441 tcataaaata atgaaagaac gagaaagaga attagctaag taaaagtcag gagaaccctg
    10501 atttttcttt actggaagtg gcctttagat tagaagactg aaaataagtt aaagtcttaa
    10561 actaattttc acagctaaga atagtagaag ttaatctgat aaaaatcgaa aaaaccagtg
    10621 gaattccgtg tcagggtaag ttccactggt tttcatagtc tattaaagtt cgaatgaaac
    10681 ctatttatag tagattgaaa ctagaatagt acacctttaa ttctaaaaca ttgttagaaa
    10741 tcgatttgac tgtcctgatc tattcgtcct attcttat
//
LOCUS       DAVLSL010000064        10758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLSL010000064 DAVLSL010000000
VERSION     DAVLSL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.065"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            92..2305
                     /locus_tag="VXN30_001257"
     CDS             92..2305
                     /locus_tag="VXN30_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1357878.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(2527..3003)
                     /locus_tag="VXN30_001258"
     CDS             complement(2527..3003)
                     /locus_tag="VXN30_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1357879.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQR
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            3226..6429
                     /locus_tag="VXN30_001259"
     CDS             3226..6429
                     /locus_tag="VXN30_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1357880.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            6809..7194
                     /gene="tnpA"
                     /locus_tag="VXN30_001260"
                     /pseudo
     CDS             6809..7194
                     /gene="tnpA"
                     /locus_tag="VXN30_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(7573..8202)
                     /locus_tag="VXN30_001261"
     CDS             complement(7573..8202)
                     /locus_tag="VXN30_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1357881.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(8212..9213)
                     /locus_tag="VXN30_001262"
     CDS             complement(8212..9213)
                     /locus_tag="VXN30_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1357882.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(9244..9885)
                     /locus_tag="VXN30_001263"
     CDS             complement(9244..9885)
                     /locus_tag="VXN30_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1357883.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(9904..10719)
                     /locus_tag="VXN30_001264"
     CDS             complement(9904..10719)
                     /locus_tag="VXN30_001264"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1357884.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         3373 a         1905 c         2331 g         3149 t
ORIGIN      
        1 taacagatag aacattgcat tcagaataat gaaaaacgca tcaaaaatgg tgcgttctgt
       61 ttttatgcta ccataaaagg aggaaaacaa attgaaaatt tttaagggag agttttatcg
      121 aatctctgta ttaacagaca agctagtaag gttagaatac tctcgaactg gaaattttga
      181 ggatagaacg acacaactta tctataatag agattttggc caaatttcgt tagattatat
      241 cgagacatca aacgtactag atattatgac ggactatttt catctgcact ttaataaagg
      301 agaatttaac gccgaaaatt tatttataga attaaaagga aattttgccg tatatggtag
      361 tcgctggtat tttggtgaat ctattgaaac gttaaaagga acagctcgga ctctggataa
      421 ggcagatgga gcaatctcgt tagaagatgg aattattagc cgaaatggta tagccttatt
      481 ggatgattct caaggattta tttgggatga acaatctggt tatattgaga gagaaaatca
      541 aattgacctg tatttctttg cctatgggca tgattataga ggagcaatca gagactttta
      601 ccatttgact ggttcaacac ccttgttgcc aagatatgct ttaggcaatt ggtggagtag
      661 atattggcct tatacgtcgg atgaatactt ggatttaata gacagatttg aaacagagaa
      721 aattccatta tctatcggtg tgttagatat ggattggcat ataactgaca ttccagctcg
      781 ttttggaagt ggctggacag gatatagttg gaatagaaac ttaataccaa atccagaaca
      841 gttattgcaa caacttcatg atagaaagtt aaaactctcc ttaaatgtcc atcctgctga
      901 tgggatacgg gcttatgaag aagcttatcc tcaaatcgca aaacggttgg ggttaaatgt
      961 agaactagaa gaacctgcta tttttgattt ttttaatccc tcttttaggg aagcctactt
     1021 taaagatgtt cattatgagc tagaaaagca gggagtagat ttttggtgga ttgactggca
     1081 acaagggaca caaggtatgc tagatccact ttggctttta aaccattatc actatcagga
     1141 tagttgtaaa aatgcagaag gtggtttgat tttatcaaga tatgcaggtc ctggtagtca
     1201 ccgctaccct gttggttttt caggggatac tattattagt tggaattcct taaggtttca
     1261 accctatttt acagcgacag catctaatat cggttatagt tggtggagtc atgatatcgg
     1321 tggacatatg ttgggggatt atgacgaaga gctacaaact agatggctac agtttggtgt
     1381 ttttagtccg attactcgat tacatagttc tagaagtcct tttaatagta aagaaccttg
     1441 gtttttttca gaaacaacat ctaagattat gaagaaatac cttcgtttga gacatcagat
     1501 gattccttat ctatatacca tgaatgtaaa gacacatgag gaaggtgccc cattaatcag
     1561 tccaatctat tatttctatc cagagaatga tgagagctat aatgttccaa accaatactt
     1621 ttttggaaca gaactgatgg tggctcccat tgtagaaaag atggatttga cattccaatc
     1681 tgcaaaagta gatgtatggt tccctgaagg tgaatggtat gacttctttt cagagaaaaa
     1741 atacacaggt ggtgtgaagt taagtgttta tagggacatc tcgactacgc ctgtgtttgc
     1801 aaaaagtggt gcaatcattc ccttggttgg ttctgagata ggtatgggtg ttgatttacc
     1861 tgaggttgta gattggtatg tattcccagg gaaacaacat tcttttgaaa tgcttgaaga
     1921 tcaaaatggt caaagatata aaacaagatt atcaatcgat tgggaaatgg gaatggtaga
     1981 gttagcatta caaggagatt ctagtatcgt tccaagcaat agaaaacata gaattcattt
     2041 taaaggaacg aatgtgtcta taattgaatt gccaaataag aatgatacag ctaaatttga
     2101 atggaaagat aataaaagga catctttaaa tgacgaagtt tttagactac taaagacagc
     2161 ttctcttcca tatgaattaa aagatagatt gttaaatcaa ttcatcaatg cgaaaaactc
     2221 tcatgactta atgaatatct tgcatcatca ggataaggaa ttgagagggc gtttgttgga
     2281 aatgatattt actagccaaa actaattgaa ataattagaa agtcatttgt ttagtgtata
     2341 tgaagattct gtatggtatt cgttatcact ggtcacattg aaaatgtgaa ataagcacct
     2401 acaacataga ctttacaaca cttcatgttt catatcatgg ggtgtttttc tatttaagtc
     2461 aaagccaatc aaaaaatctc actattcatc aggttaaagg aaacctaatc atagtgagat
     2521 tgtgaattat agtagagttt gtatcgcctc ttcaatttgt tttgggtctg tttttgaaga
     2581 aaagcgttca aagacttgcc catcacgacc aatgagaaac ttagcgaaat tccattcgat
     2641 tcgttttcct agtgggccag atttatggtc ttttaaccag acatagagag ggtctgcttc
     2701 cttaccgttg accttaatct tggcaaaacg tgggaaggtg gtttgaaaat gtaggctaca
     2761 gaaggtgttg atttcctctg cgctgccggg tgcttgtccc ataaactgat tgcaagggaa
     2821 atccaatatt tcgaagccct gatcttgata gcgttcatag agttcttgaa gtctctggta
     2881 ctggggcgtt aaaccacatc cagtagcagt gttgacaatc aagagaactt tgccacgata
     2941 gctatccaag ggagttgctt ggttgttttg gttcaaaacg gaaaaatcat aaagtgaagt
     3001 catgagggcc tcttttcttt ttctacattg taacagtttt tacttttttc gtcgagtcgg
     3061 atggaagata ttgttgaaga ataagccctc aatatgatag aaaccgttta caaataactt
     3121 taaaaatggt atgatttaat taaaccggtt tactttgttg ctataaatct ctaatttgag
     3181 atttagttcc tattgatttt acaatatgtt tattggagtg tatacatgca aacaaaaaca
     3241 aagaagctca ttgtgagttt gtcttcactt gttttatcag gatttttatt aaaccattat
     3301 atgacagttg gagcggaaga aacgactacg aataccattc agcaaagcca gaaggaagtt
     3361 cagtatcagc aaagggatac aaaaaattta gttgaaaatg gtgattttgg tcagacggag
     3421 gacggaagca gtccgtggac aggaagcaaa gctcaggggt ggtcagcttg ggtagaccag
     3481 aagaatagtt ctgcagatgc ctcaactcga gtcattgagg ctaaggatgg ggctatcact
     3541 atctcaagcc ctgagaaatt aagggcagcg gttcaccgta tggttcctat tgaagctaag
     3601 aaaaagtata aactgcgttt caagattaaa acagataata aagtcgggat tgccaaggtt
     3661 cgtatcattg aggaaagtgg taaggacaag cgattgtgga attctgcaac gacgtcagga
     3721 acaaaggact ggcagaccat tgaagcagac tatagcccga ctttagatgt tgataaaatc
     3781 aagctggagt tattctatga aacaggaact gggactgttt cctttaagga tattgagctg
     3841 gtagaggtag cagcccagct ttctgaggat tctcaaacag ataaacagct tgaggaaaag
     3901 attgatttac caattggaaa aaaacatgtt ttttctcttg cggactatac ttataaggta
     3961 gaaaatcctg acgttgcttc agtcaaaaat ggaattttag aacctcttaa ggaagggaca
     4021 accaatgtca ttgtcagtaa agatggcaag gaagtgaaaa agattccttt gaagattcta
     4081 gcctctgtta aggatgcata cacagcccgt ttggatgact ggaatggcat catcgctggg
     4141 aatcaatact atgattctaa aaatgaacag atggccaaat taaaccagga attggaagga
     4201 aaggtagctg atagcctatc tagtatttca agtcaggcgg accgcaccta tttgtgggaa
     4261 aaattttcaa attataaaat gtctgcaaat ctgactgcca cttatcggaa attggaggag
     4321 atggccaagc aagtgaccaa tccttcttct cgttattatc aagatgaaac tgtcgttcga
     4381 acagtcaggg attccatgga atggatgcat aaacatgtct acaatcgtga aaagagcatt
     4441 gttgggaact ggtgggatta tgaaatcggt acacctcgtg ccatcaacaa taccttgtct
     4501 ctgatgaaag aatacttctc tgatgaggaa attaaaaaat atacagatgt gattgaaaaa
     4561 tttgtaccag atcccgaaca tttccgaaag acgactgata acccatttaa ggctctaggt
     4621 ggaaacttag ttgatatggg aagagtaaaa gtaatagctg gtttactgcg taaggatgat
     4681 caagaaattt cttctaccat tcgctcgatt gagcaagtgt tcaagttggt agaccaaggt
     4741 gaaggttttt atcaagatgg atcctatatc gaccacacca atgttgccta tacgggtgct
     4801 tatgggaatg ttttgattga tggcctgtct caactgttgc cagtcattca aaagaccaag
     4861 aatccaatcg ataaagataa aatgcaaacc atgtaccact ggattgataa atcgtttgct
     4921 cctttgctgg tgaatggaga gctgatggat atgagtcgtg gacgctcgat cagtcgtgca
     4981 aatagcgagg ggcacgtggc cgcagtagaa gtactaagag ggattcaccg aatagcggat
     5041 atgtctgaag gagaaaccaa acaacgtttg cagagtcttg tgaagaccat tgttcaatcg
     5101 gatagttatt atgatgtctt taagaatttg aagacttata aggatatcag tttgatgcaa
     5161 tccttgttaa gtgatgcagg agtcgcaagt gttccaagaa caagttacct atctgccttt
     5221 aacaagatgg ataaaacagc catgtacaat gcagagaaag ggtttggatt tggcttgtca
     5281 ctcttttcca gtcgtacctt gaattacgaa cacatgaaca aggaaaataa acgtggttgg
     5341 tatacgagtg atgggatgtt ctatctttac aatggcgatt tgagtcacta tagcgatggc
     5401 tactggccaa cagttaatcc atataagatg cctggtacaa cagagacgga tgctaagaga
     5461 gcggatagcg atacaggtaa agttttaccg tctgctttcg ttggaacgag caaactagat
     5521 gatgccaatg cgacagcaac catggatttc accaactgga atcaaacatt gactgctcat
     5581 aagagctggt ttatgctaaa ggataagatt gcctttttag gaagcaatat ccaaaacact
     5641 tcaacagata ctgctgcaac tacaatcgac cagagaaaac tggaatcaag taatccatat
     5701 aaagtctatg tcaatgataa agaagcctcc cttacagaac aagaaaagga ttatcctgaa
     5761 acccaaagtg tctttttaga atcgtccgat tcgaaaaaga atattggtta ctttttcttt
     5821 aagaagagtt caatcagcat gagtaaggct ttgcaaaagg gagcctggaa ggatatcaat
     5881 gaaggacagt cagacaagga agttgaaaat gaatttctta cgattagtca ggctcataag
     5941 caaaatggag attcttatgg ctatatgctc attcctaacg tggatcgtgc caccttcaat
     6001 caaatgataa aagagttaga aagtagcctc atcgaaaata acgaaaccct tcagtctgtt
     6061 tatgatgcca aacaaggagt ttggggcatt gtgaaatatg atgattctgt ctctactatt
     6121 tccaaccaat tccaagtttt gaaacgtgga gtctatacca ttcgaaaaga aggggatgaa
     6181 tataagattg cctactataa tcctgaaacc caggaatcag ctccagatca ggaagtcttt
     6241 aaaaagctag agcaagcagc tcagccacaa gtacagaatt caaaagaaaa ggaaaaatct
     6301 gaagaggaaa agaaccattc ggatcaaaag aatctccctc agacaggaga aggtcagtca
     6361 atcttggcaa gtctagggtt cttgctactt ggggcgtttt atctattccg tagaggaaag
     6421 aacaactaat ttgttcatca tctagatgaa ttacatgaaa ctgtggaaaa tagaaaaaat
     6481 ccgaagaagg ccaaatatct tcggattttt cttttaaaaa aggataagtt gatacttatc
     6541 ctttttactt cattatgtga ctatagcgtt ttgaaactag aataggacat cataactgct
     6601 aaaagatttc tataaattca tttgattttc ctaatcaatt tgttcgtatc ctatttcact
     6661 ccactatatt agtccgtctc gctccgtgag gtgcgagaca aaacaaccac ccgctatacg
     6721 ggtgcgcgtc gaaggttata ccaaaaaaac tccaaacgcg atacaataaa ggtgttcaat
     6781 ccacttgtaa agcgaaagga gaaaaaatat ggcacaaaag gctcatagtt tatctcacac
     6841 aaagtggagg tgttctatca cattgtgttc acccctaagt atagacgaaa agtcatctat
     6901 aatcaatata gaagtagttt aggcgaaata tttcatcgct tgtgtagtta taaaggtgtt
     6961 gaaatgatcg agggtcactt aatgccagac catgtacaca tgttagtcag tattccgtcg
     7021 aggctaagtg tttcaagttt tatggggtat ttaaaaggca aaagtgcact catgatgttt
     7081 gacaaacacg ccaatctcaa gtacaagttt gggaatcggc atttctgggc ggaaggttat
     7141 tatgtaagtc cagtagggct taatgaagcc acaattaaga aatatagtca agattaaaga
     7201 aattatccag tggatgattt cttcacgagt atgaaaattt gagaacgagt aaagtatgat
     7261 atatagtaaa atgaaataag aacaggacaa aatcgatcag gacagtcaaa tcgattttta
     7321 acaatgtttt agaagtatag gtgtactatt tcagtttcaa tctactatag cactagataa
     7381 attgagtgta aaagaatatg aggatccctt tagggatagt ggtaagtaat accaaagtct
     7441 cttaaagagg caagtgacga gtcaagagca ataaggcttg aacaacgtga aagccagcgt
     7501 ctttaggcgc tggctgatga tttgggctta tagctctgag ataaaccacc cgttagacgg
     7561 gtagttatga ttttatctga gtgtaatata ctgttgggca atctcgctga tgcggtcaaa
     7621 gttgccttgg gaagcgagtt tattgagttc gccaccaatt ccaacggcat ctgcaccagc
     7681 agcgaaccat tgagggatgt tgtttaggcc gactcctccg gttaccatta cggaaacttg
     7741 tgggatcggt gccttgactg cagagatata tgctggactg agagtactac ctgggaagag
     7801 tttgatgatt tcactaccgg cttcaagtgc agtcgtgatc tctgtgaggg taatacagcc
     7861 tggaatgtac ggtgtgctgt agagattgca cattttcgca gtttcagcat ggaaagatgg
     7921 agaaacaacg taatttgctc cagctagaat ggcatctcta gcagttacgg catcaagcac
     7981 agtacctgca ccgatacaaa cactctgatc gtcctgatac aagtctacaa gttccttgat
     8041 gatttgtcct gcatactgat tggtataggc gatttcaata gctttgatac cgcccttgat
     8101 acaagcaatc gaggcttgta gtccttcttc ctttgtattt cctcgaatga cagcgacaat
     8161 tttctgtttt tttagttcaa taatcgtatc tgatttggtc atgtaattct cctaacgaat
     8221 gatatcttgt gcatttgcca gtaaattttc aatactagtt gcggaagtgg agagatggtc
     8281 tcctggaaga gtgcatttga gagttgcgct cgcaactgca aagtcaatgg tagtttttag
     8341 ggaggaatga tggagtagtt ggtaaagggc accagatata aaggcatccc cgctaccaat
     8401 tcgttgatag actgcagttt ttagttggac agactcttca aatcgttctt ccagagcata
     8461 ggcttgatag acatttttgt cttgctcatc actagatcgg agggtatgga aaatagcctt
     8521 gaaaccatag gcttctttta aaagtcgcat gcgattttcc acctcttcta ggctagcact
     8581 gtctcttggg aacatctcta gattttggtc atcaatcatg agaggatcaa taccgaagca
     8641 atagtcagta aaacgtgcaa acttagaaaa ttcatacttg gcttctagga ctgaaatcat
     8701 ctttgttctc agattgagat ccattgaaac gacaattcct cggcgcttgg cttcttccaa
     8761 gagtaggaga aggatcgcac ggacctcttg accgatagct atggtgattc cactaaaatg
     8821 aaaatggcta atcccctgaa agagagaatc catatctagc atgtttggcc gaatctggct
     8881 gatactcgta tgcttacgat cgtagaaaac ttcactttga cgacaaccaa agccgttctc
     8941 caaatagtag aggccgattc gatcgccaag ccgacagatt gaactggtat cgatttggtg
     9001 ctgtttcaag aatgtgagaa aacgatctcc aatctcgttg gcagggagtg cggtaaagac
     9061 tttcgttgag atacccaggg cttgcaaatt acaagcgatg ttaatttctg atccgccaaa
     9121 ataagtcgaa ctggcaacat gatcgccgat actggtggca tctaatggtg agattcgaat
     9181 taatggttcg ccaaacagta atattttcgt catccattag ttcctttctg gtttagaata
     9241 gctttacttg gctaatactt ctttcaaata agcagcgact tcatcatctt ggcagttttc
     9301 aaagaagtat tcttggaagt atttgccaga gatggtgtct ttgaggaagt cttggtcgat
     9361 tgttttaagg atggtcatca aatcattgtg ggtgatttgt ttcacctcgt ttaagatacg
     9421 agcgttgcgt tgttcaggga ttacacgttc tcttgggtag ccaccgccca tttcttcagc
     9481 aaataagcgt tcaaacatca atttgagggt cagttctgcc ccccagccaa atcctttggc
     9541 ataagggata gacaaggcgt taccaccatt gatttgagaa taaaggtaag cgtcagttgg
     9601 gtccactgct agaccacaga caacaccagg gaagctgttt aaagcaagca tagcccctac
     9661 acccgtacca cagccggtaa caacaaagtc aactgccttt gtatttaaaa ggatggcagc
     9721 cattagtccg ttctgcacat aagttaattg actttctcct tcttctccac gcataccata
     9781 gttaaataat tggtagcctt ttttatctgt cgcttctttt agactatcgt aaataatgtg
     9841 attcttgcta gcttgactat tttcattgat taatgcgatt ttcataagat cttctttcta
     9901 tttttactca ggttgttttc cgatgtaggc taagataccg ccatctacat ataggatgtg
     9961 gccattgaca aaattgctgg catcactagc gagaaagaca gcagggccca tcaaatcttc
    10021 aggatttccc caacgtgcag caggtgtttt tgcaatgatg aactggtcaa atgggtggcg
    10081 agaaccatct tcttgcaact cacgaagagg tgctgtttga ggagtggcaa tataacccgg
    10141 tccaattccg ttacattgga tattggctcc accgtattca gacgcaatgt tgcgggtcaa
    10201 cattttcaag ccccctttag cagcagcata agcgctaact gtttcacgtc ccagttcgct
    10261 catcatcgaa caaatattga taatctttcc atgccctttc tttatcatag aaggaataac
    10321 tgcctttgaa acgataaatg gtgcgtttaa gtcaatatcg atgaccttac ggaaatcagc
    10381 ggcgctcatt tcgcacattg gaactcggcg gataatacca gcgttattaa cgaggatgtc
    10441 aatgacacca acctcttgtt cgatttgctt gaccatggct tggataccgt cctcgtctgt
    10501 cacgtcacag acatatccat gggcttggat gccaacttca cgataagctt caatcccttt
    10561 attgaccaga tcttgattga tatcgttaaa gacaatagta gcgccggctt cagcgtagga
    10621 tttggcaata gcaaatccaa ttccataaga agcgccggtg atgagagcaa tttttccttt
    10681 taaagaaaac tgctcaattg agaatgatgt atttgtcatt tgatacttcc tttcaaattt
    10741 gttaagactt ctctataa
//
LOCUS       DAVLSL010000065        10750 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLSL010000065 DAVLSL010000000
VERSION     DAVLSL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.066"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            47..418
                     /locus_tag="VXN30_001265"
     CDS             47..418
                     /locus_tag="VXN30_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357885.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            1047..1430
                     /locus_tag="VXN30_001266"
     CDS             1047..1430
                     /locus_tag="VXN30_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1357886.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            1482..2171
                     /locus_tag="VXN30_001267"
     CDS             1482..2171
                     /locus_tag="VXN30_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1357887.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            2213..2446
                     /locus_tag="VXN30_001268"
     CDS             2213..2446
                     /locus_tag="VXN30_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357888.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            2597..3208
                     /locus_tag="VXN30_001269"
     CDS             2597..3208
                     /locus_tag="VXN30_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1357889.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            3369..4163
                     /locus_tag="VXN30_001270"
     CDS             3369..4163
                     /locus_tag="VXN30_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1357890.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            4160..4795
                     /gene="trmB"
                     /locus_tag="VXN30_001271"
     CDS             4160..4795
                     /gene="trmB"
                     /locus_tag="VXN30_001271"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1357891.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4921..5400
                     /gene="rimP"
                     /locus_tag="VXN30_001272"
     CDS             4921..5400
                     /gene="rimP"
                     /locus_tag="VXN30_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1357892.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            5444..6580
                     /gene="nusA"
                     /locus_tag="VXN30_001273"
     CDS             5444..6580
                     /gene="nusA"
                     /locus_tag="VXN30_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1357893.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            6602..6895
                     /locus_tag="VXN30_001274"
     CDS             6602..6895
                     /locus_tag="VXN30_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1357894.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6888..7187
                     /locus_tag="VXN30_001275"
     CDS             6888..7187
                     /locus_tag="VXN30_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1357895.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            7204..9996
                     /gene="infB"
                     /locus_tag="VXN30_001276"
     CDS             7204..9996
                     /gene="infB"
                     /locus_tag="VXN30_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1357896.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            10247..10597
                     /gene="rbfA"
                     /locus_tag="VXN30_001277"
     CDS             10247..10597
                     /gene="rbfA"
                     /locus_tag="VXN30_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1357897.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3276 a         1931 c         2418 g         3125 t
ORIGIN      
        1 ctatttttct ttaattaaaa agaaagaaaa gaaatgaaag attttcttga ttaaaaagaa
       61 tcatattctt tgtgagattg ttcctgggtc agttgccttt gcttgtttct acttatctat
      121 ttctatttcg tcagttttcc aatttctttt agtagttatt aacttgactt ctattttggt
      181 tactgtttat cttactaggg aaatgagatt aagagagttt gaagatgatg atttggttag
      241 tcctagaacc aatcaactca tgtttatcgg cttgacaggc tttatgtcta ttatttgttt
      301 gtatagaggt atcacagcag gagaatctta tcaacaacta atcgcttata ttggtgctat
      361 tctttgcttg atcatcatgc ttctactcat ttggggtttg aagtattata aaaagtaggg
      421 gctaatgaag tttttgcaat ttaggaggtt tcgatgacaa ttcaagattt ggatgaaaaa
      481 ttttaaaaaa caatgatata ctaaacttgt caaagttgca acgggtgctg ctacattatg
      541 ttaaaaatcg ctcttgattg ataagttgga tttgttagga aaggagtaaa taattatgga
      601 taagaaaaag atagtttcga ctataatatg tatagtattt cttgtagtgt cagtagataa
      661 tttttttaga gacttaaccc cgttactgtt cattttaaat attattggcc tgtcttgctt
      721 ttcagtgcta acatacatta acataaaaga gatactctta aatataagta aatgatgatt
      781 tggttagtcc tagaaccaat caacttatgt ttatcggctt ggcagatttt atgtttgtca
      841 tttgtttgta tagaggtatt tcagaaacag aattctatca acaactaatc gcttatatag
      901 gtgtattctc tgcttgttta tcacgcttct gctcatgtgg ggcttgaagt attataaaaa
      961 gtagctttct atccttttct gacaggatat ttgagtagaa ggctctatta aattgttagt
     1021 gaaataaatg aatggaggta tttaatatga aatacagatt attttttgtt attttcttga
     1081 gtagtgtgtt ggatattctt ttagggacat ttttacaaat ctctatcgta tctattgggt
     1141 ggcttgttct ttacagtggt ttgtttgaag caggagtttt ccttcttgct aataaagggg
     1201 tggcggtaaa aatcaaggaa gtagatattc gaaatcgctt taaatttatt tttggaaaaa
     1261 ccttatggtt tcaaattctt ttgctcatct ttttgataat caaactttat cttggtttgg
     1321 atgcgaggtt gattttattc tatggacata ttttcattgt ctttaatgcc ttaatgtatc
     1381 tattatctag tagtcaggtt agccttaaaa aaaacaaact gtcttcttaa tcttacagca
     1441 gtagagcctg acaaggtgag gtcatagtag ggaggatttc tatgaaaaag tatcaacttc
     1501 tattcaaaat aagtgcagtt ttctcttact tatttttcgt atttggtctt tctcagatga
     1561 cgcttattat tcaaaattat tggcaatttt cttcccagat tggcaatttc gtctggattc
     1621 aaaatttctt gagtttgcta tttagcggag tcatgatttg gattctggtt aagacaggtc
     1681 atggttatct ctttcacatt ccaagaaaaa aatggctttg gtattcgatt ttgacagtat
     1741 tagtggtagt gctccagatc tcttttaacg ttcagacagc taaacatgtt cagtcaactg
     1801 ctgaaggttg ggctgtattg atcggttata gtgggaccaa ctttgctgag ctaggtatct
     1861 atataacttt gttctttctg actccactta tggaagagct aatctataga ggattactgc
     1921 aacacgcctt ttttaagcat tcgagatttg gccttgattt gcttcttccg tcaattttat
     1981 ttgctcttcc tcatttttca agcctgccta gtttgttaga tatcttcatc tttgcaacat
     2041 ctggcatcat ctttgctagt ttgacccgct ataccaagag catttatcct tcctatgcgg
     2101 tgcatgtgat caataatatt ttcgcaacat taccattttt gctgactttt ttacataggg
     2161 tcttggggta aaaaaacaaa aggcttgctt ttcagccata gaggagatta tcatgtataa
     2221 acacttattt ttcctagatt ccaaaacttt agatcggttg acaccctata ttctagtctt
     2281 ggcttctgac accattgctt ttaatgtttt tgtgctaacc tttgtatctg cggtggtttt
     2341 taatttccta aattccatgc tagctttaat ggctatattc ataggggctg gctatgtggt
     2401 cggattttgg ttactaatac tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag
     2461 gctgtacttg agtacggcaa ggcgacgttg acgtggtttg aatttgattt tcgaagagta
     2521 taaaatggtt tgttttggaa aggctagaac taaagaatga cgcgtaggaa gtctgtttgg
     2581 tgaggaggat agttttatgg agttttttga taaatttcat gccttgtgtt ttggattttt
     2641 agtactaata attgtcatta cagttcctta tacgattaac catgggggtt tttttcaaaa
     2701 tgaatctgca ttgattcttg taagtcttct tgtaacctcg ctgagtgttg cttatgctag
     2761 aaagtttgaa atgatttctt ttgggatgtt aagcaagaaa caacttttgc ttttcattgc
     2821 aatctttctt ctaagtgtac ttgagacgct ggtttatatt catttcttcg ctgtttcttc
     2881 tggctcaggg gtccaacact tggcggaagt cagcagagga atttccctgt ctttgatttt
     2941 gactacctca gtttttggcc ccatccagga ggaactcatt ttcagaggac ttcttcaagg
     3001 tgcggttttt gacaattctt ggttagggct tgtgctaact tcctctctct tttctttcat
     3061 gcatggacct tctaatgtcc cttcgtttat tttttatcta cttgggggtt tgttgctggg
     3121 ctttgcttat aaaaagagtc aaaacctatg ggtttctact ctagtccaca tgttttacaa
     3181 cagttggcca ctcctatatt atttataaaa attatgaaaa ggtaagcaga agatggtgct
     3241 tacctttttc aagctataat gatacccaag ccaatccaga ggcttttctt tgagaatcgg
     3301 gttagagtgg ttgacgaata ggccaaaaac tagtagaata gtaaggaaac tttatacgga
     3361 ggaaagaaat ggatttgggt gataatgagc taacactgac tcccatacct gggaaaagtg
     3421 gcaaggctta tatgggtagc tatcctgacg ggaagcgcat ctttgtaaaa atgaacacct
     3481 ctccaatcct acctggtcta gctagagaac aaattgctcc acaattatta tggagtcgtc
     3541 gtttggcaga tgggcgtgat atgtgtgctc aagaatggtt gacaggcaag atattgaccc
     3601 cctatgatat gaatcgtaag caaatcgtca atattttaac ccgtcttcat cgctcacgtc
     3661 cgttgatgac acaattgagt cgtttgggct atgccatgga aacacctgta gatttactac
     3721 agtcttggca ggaaacggct ccagatgctt tgcgtaaaaa tcattttatc agtgaagtga
     3781 tggctgattt acgtcagact attccaggat ttagagagga ccatgcgacc attgtccatg
     3841 gagatgtacg acatagtaat tggattgaga cagatagtgg cttgatttat ttagtagatt
     3901 gggattcggt tcgcttgacc gatcgcatgt ttgatgtggc ccatatgctc tgccattata
     3961 tttcagaaca tcagtggaag gaatggttga cctactacgg ttacaagtac aatcaaacgg
     4021 tattaagtaa attgtattgg tatggtcaat tgtcttattt gagtcagatt tccaagtatt
     4081 atatgaacca agatttagaa aatgtcaatc gggagattca tggtttgcgt catttccgag
     4141 acaagtatgg aaagagaaga tgagagttag aaatcgtaaa ggggcaacag aattactaga
     4201 ggcaaatccc cagtatgtgg tcctcaatcc cttggaagcc aaggcaaaat ggcgggactt
     4261 gtttggcaat gataatccca ttcatgtgga agttggaagt ggaaagggtg cctttgtttc
     4321 aggtatggcc aagcaaaacc ctgacatcaa ctatatcggg attgatattc aaaagtctgt
     4381 tttgagctac gctttggaca aggtgcttga agttggagtg cctaacatca agctcttgtg
     4441 ggtagatggt tctgacttaa ctgactattt tgaagacggt gagattgatc gcttgtatct
     4501 gaacttttca gatccatggc cgaaaaaacg ccatgaaaag cgtcgtttga cctacaagac
     4561 cttcttggat accttcaaac gtatcttgcc tgaaaatgga gaaattcatt tcaagacgga
     4621 taaccgtggc ttgtttgagt acagtttagt gagcttttct caatatggca tgaaactcaa
     4681 tggtgtctgg ttagatttgc atgccagtga ttttgaaggt aatgtcatga cagaatacga
     4741 gcaaaaattc tcaaacaagg ggcaagttat ctaccgagtt gaggcagaat tttaagagat
     4801 agcctaaaat taggctgtac aagtgctttt gctttacata agttggcaaa cgtgctatac
     4861 tgatagtaag aatatgaaaa gtgaggcggg gaaatatctt cgcctcttgc ttatgaggag
     4921 gtggacgcaa tcgcaacaat cgtagaatta gtcagagaag ttgtagaacc tgtcatagaa
     4981 gctccttttg aactcgtgga tatcgagtat ggaaagattg gcagtgacat gattctcagt
     5041 atttttgtag ataaacccga aggaattacc ttgaacgaca cggcagactt gacagaaatt
     5101 atcagtcctg tcctagacac catcaagcca gatcccttcc cagaacaata tttcctagaa
     5161 attaccagtc caggtttgga acgccctttg aaaaccaagg atgccgtcgc tggagcggtt
     5221 ggaaaataca tccatgtcgg gctctaccaa gccatcgata agcaaaaggt ctttgaagga
     5281 accttgttgg ccttcgaaga ggacgagttg actatggaat atatggacaa gacgcgtaag
     5341 aaaaccgtcc aaattccata cagtttagta tcaaaagcac gtttagcagt taaattatag
     5401 aaaaagaaag gatagctttt gaggattcaa aagtgaagaa aacatgagta aagaaatgct
     5461 agaggccttc cgcattttgg aagaagacaa gggaatcaaa aaagaagata tcatcgacgc
     5521 agtagtagag tcgcttcgtt ccgcttatcg cagacgctat ggtcagtcag acagcgtagc
     5581 tattgacttc aacgaaaaaa caggtgactt tacagtttat actgtccgtg aagttgttga
     5641 tgaagtattt gatagccgtt tggaaatcag cttgaaagat gctcttgcca ttaattcagc
     5701 ttatgaactt ggagacaaaa tcaagtttga agaagcacca gctgagtttg gtcgtgtagc
     5761 agcccagtct gccaaacaaa ccatcatgga aaaaatgcgc aagcaaacac gtgccatcac
     5821 ttacaatact tacaaagaac atgagcaaga aatcatgtct ggtacagtag aacgctttga
     5881 caaccgcttt atctatgtca accttggtag catcgaagcc caattgtcaa aacaagacca
     5941 aattcctgga gaagtttttg cttctcatga tcgtatcgaa gtttatgttt acaaggttga
     6001 agacaaccct cgtggtgtga atgtctttgt tagccgtagt catccagaaa tgatcaaacg
     6061 tttaatggag caagaaattc cagaagttta tgatggaact gttgaaatca tgagcgtggc
     6121 tcgtgaagca ggtgaccgta cgaaggttgc tgttcgtagc cacaatccaa acgtggatgc
     6181 tatcggtaca atcgttggac gtggtggtgc taatatcaag aagattacta gcaaattcca
     6241 cccagctcgt tacgatgcta aaaatgaccg catggtacca atcgaagaaa atatcgatgt
     6301 tatcgagtgg gtagcagatc cagctgaatt tatctacaat gccatcgctc ctgctgaggt
     6361 tgaccaagtt atctttgatg aaaacgacag caaacgtgcc ttggtggttg ttccagataa
     6421 caagctttct cttgccattg gtcgtcgtgg acaaaacgtg cgcttggcgg ctcacttgac
     6481 tggttaccgt atcgatatca agtctgctag cgaatttgaa gccatggaag acgctgcttc
     6541 agtagagttg gaagtagaaa acgatactgt agaagaataa aagctgctag aggagggaaa
     6601 gatgaaaaca agaaaaatcc ctttgcgcaa gtctgttgtg tctaacgaag tgattgataa
     6661 gcgtgatttg ctccgcattg tcaagaacaa ggaaggacaa gtctttattg atcctacggg
     6721 caaggccaat ggccgcggcg cttatatcaa actagacaat gcagaagccc tagaggcgaa
     6781 aaagaagaag gtctttaacc gcagctttag catggaagtg gaagaaagct tttatgacga
     6841 gttgatcgct tatgtggatc acaaagtgaa aagaagagag ttgggacttg aataagcaaa
     6901 agataagtaa tctcttgggg cttgctcagc gagcagggcg catcatatcg ggtgaagaat
     6961 tggtggtcaa ggccattcaa gacggcaagg ccaagttggt ctttctagct catgatgctg
     7021 gacccaatct gaccaagaag attcaagata aaagtcatta ttatcaagta gaaattgtaa
     7081 ccgtgttttc aacactggaa ttaagcatag cagtcgggaa atcgagaaag gttttggctg
     7141 taacagatgc tggatttaca aagaaaatga ggtctcttat ggaatagaag aggaggacat
     7201 gatttgtcta agaaaagatt gtacgaaatc gcaaaagaac ttggaaaaga aagtaaagaa
     7261 gttgtagcgc gtgcaaaaga gttgggcttg gatgtgaaaa gccactcatc aagtgtggaa
     7321 gaagctgtcg ctgcaaaaat tgctgccagc tttaagcctg cagctgctcc gaaagtagaa
     7381 gcaaaacctg cagcaccaaa agtaagtgca gaaaagaaag ccgaaaaatc tgagccagct
     7441 aaaccagctg tagctaagga agaggcaaaa ccggctgagc cagttgctcc gaaaacagaa
     7501 aaagtagcag tgaaaccgca aagtcgtaat ttcaaggctg agcgtgaagc ccgtgctaaa
     7561 gagcaggcag aacgacgcaa gcaaaataag ggcaataacc gtgaccaaca acaaaacgga
     7621 aaccgtcaga aaaacgacgg ccgtaatggt ggaaaacaag gtcaaagcaa ccgcgacaat
     7681 cgtcgcttta atgaccaagc taagaagcag caaggtcagc aaaaacgtag aaatgagcgc
     7741 cgtcagcaag aggataaacg ttcaaatcaa gcggctccac gtattgactt taaagcccgt
     7801 gcagcagccc taaaagcaga gcaaaatgca gagtacgctc gttcaagtga ggaacgcttc
     7861 aagcagtatc aggctgctaa agaagccttg gctcaagcta acaaacgcaa ggaaccagag
     7921 gaaatctttg aagaagcggc taagttagct gaacaagcac agcaagttca agcagtggtt
     7981 gaagtcgtcc ctgagaaaaa agaacctgca gtggatacac gtcgtaaaaa acaagctcga
     8041 ccagacaaaa atcgtgacga ttatgatcat gaagaagatg gtcctagaaa acaacaaaag
     8101 aatcgaagta gtcaaaatca agtgagaaat caaaagaata gtaactggaa taacaacaaa
     8161 aagaacaaaa aaggcaataa caagaacaac cgtaatcaga ctccaaaacc tgttacggag
     8221 cgtaaattcc atgaattgcc aacagaattt gaatatacag atggtatgac cgttgcggaa
     8281 atcgcaaaac gtatcaaacg tgaaccagct gaaattgtta agaaactttt catgatgggt
     8341 gtcatggcca cacaaaacca atccttggat ggggaaacaa ttgaactcct catggtggat
     8401 tacggtatcg aagccaaaca aaaggttgaa gtggataatg ctgacatcga acgtttcttt
     8461 gtcgaagatg gttatctcaa tgaagatgaa ttggttgagc gtccaccagt tgttactatc
     8521 atgggacacg ttgaccacgg taaaacaacc cttttggata ctcttcgtaa ctcacgtgtt
     8581 gcgacaggtg aagcaggtgg tattactcag catatcggtg cctaccaaat cgtggaaaat
     8641 ggtaagaaga ttaccttcct tgatacacca ggacacgcgg cctttacatc aatgcgtgcg
     8701 cgtggtgctt ctgttaccga tattacgatc ttggtcgtag cggcagatga cggggttatg
     8761 cctcagacta ttgaagccat caaccactca aaagcagcta acgttccaat catcgtagcc
     8821 attaacaaga ttgataaacc aggtgctaac ccagaacgcg ttatcggtga attggcagag
     8881 catggtgtga tgtcaaccgc ttggggtgga gattctgaat ttgttgaaat ctcagctaaa
     8941 ttcaaccaaa atatcgaaga attgttggaa acagtccttc ttgtagctga aatccaagaa
     9001 ctcaaggcag acccaacagt gcgtgcaatc ggtacggtta tcgaagcgcg cttggataaa
     9061 ggaaaaggtg cggtcgcaac ccttcttgta caacaaggta ccttgaatgt tcaagatcca
     9121 atcgttgtcg gaaatacctt cggtcgtgtc cgtgctatga ccaacgacct tggtcgtcgt
     9181 gttaaagttg ctggaccatc aacaccagtc tctatcacag gtttgaacga agcaccgatg
     9241 gcgggtgacc actttgccgt ttacgaggat gaaaaatctg cgcgtgcagc aggtgaagag
     9301 cgtgccaaac gtgccctcat gaaacaacgt caagctaccc aacgtgttag ccttgaaaac
     9361 ctctttgata cccttaaagc tggggaactc aaatctgtta atgttatcat caaggctgat
     9421 gtacaaggtt ctgttgaagc cctttctgcc tcacttcaaa agattgacgt ggaaggtgtc
     9481 aaagtgacta tcgtccactc agcggtcggt gctatcaacg aatcagacgt gacccttgcc
     9541 gaagcttcaa atgcctttat cgttggtttc aacgtacgcc ctacaccaca agctcgtcaa
     9601 caagcagaag ctgacgatgt ggaaatccgt cttcacagca ttatctacaa ggttatcgaa
     9661 gagatggaag aagctatgaa agggatgctt gatccagaat ttgaagaaaa agttattggt
     9721 gaagcggtta tccgtgaaac cttcaaggtg tctaaagtgg gaactatcgg tggatttatg
     9781 gttatcaacg gtaaggttgc ccgtgactct aaagtccgtg ttatccgtga tggtgtcgtt
     9841 atctatgatg gcgaactcgc aagcttgaaa cactacaaag acgacgtgaa agaagtgaca
     9901 aacggtcgtg aaggtggatt gatgatcgac ggctacaatg atattaagat ggatgatgtg
     9961 attgaggcgt atgtcatgga agaaatcaag agataagatt ttttgctcct ttcttaggtg
    10021 gtgagggacg caagcaaacc gatggtttca ttgcttattt ttgagcctag ggtctcaaaa
    10081 atcccctgtg atgggactga taaatcagtt ccatcacttt caccacggcg aaagaagcag
    10141 atgacttcaa attgaacttc gtttcaattt aaactgaaaa tcaagaagtt taaaatagct
    10201 aggtctgctg gcctagcttt tggttcaaag tagagaaagg aatatcatgg caaatcattt
    10261 ccgtacagat cgtgtgggca tggaaatcaa gcgtgaagtc aatgagattt tgcaaaagaa
    10321 agtccgtgat ccacgtgtcc aaggtgtgac catcacagat gttcagatgc tgggtgactt
    10381 gtctgttgcc aaggtttatt acaccatttt gagtaacctt gcttcggata accaaaaagc
    10441 ccaaatcggg cttgaaaaag caactggtac catcaaacgt gaacttggtc gcaatttgaa
    10501 attgtacaaa atcccagatt tgaccttcgt caaagacgag tccatcgagt atggaaacaa
    10561 gattgacgag atgctacgca atctggataa gaactaaaga agaggggttg cccctctttt
    10621 ttggtggagg aaaataggtt gaatttgaaa tggaaaaata ttcttttaaa atatattgaa
    10681 actagaatag tacgcctcta cttctaaaac attgttagaa atcgatttga ctgtcctgat
    10741 cgatttgtcc
//
LOCUS       DAVLSL010000066        10553 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLSL010000066 DAVLSL010000000
VERSION     DAVLSL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.067"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(49..1371)
                     /locus_tag="VXN30_001278"
     CDS             complement(49..1371)
                     /locus_tag="VXN30_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1357898.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALMVLSPVVIAETKKYFDK"
     regulatory      complement(1462..1550)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1714..4050)
                     /locus_tag="VXN30_001279"
     CDS             complement(1714..4050)
                     /locus_tag="VXN30_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1357899.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLGPTVKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGAALNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESRVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4154..4702)
                     /locus_tag="VXN30_001280"
     CDS             complement(4154..4702)
                     /locus_tag="VXN30_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1357900.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVINLIG"
     gene            complement(4699..5001)
                     /gene="zapA"
                     /locus_tag="VXN30_001281"
     CDS             complement(4699..5001)
                     /gene="zapA"
                     /locus_tag="VXN30_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1357901.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5088..5969
                     /locus_tag="VXN30_001282"
     CDS             5088..5969
                     /locus_tag="VXN30_001282"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1357902.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQITPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSKPISLEEKAEG
                     KYLVVAVSSIIARNLFLENLENLGRELGYQLPSGAGTTSDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            5973..6587
                     /gene="lepB"
                     /locus_tag="VXN30_001283"
     CDS             5973..6587
                     /gene="lepB"
                     /locus_tag="VXN30_001283"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1357903.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6715..9081
                     /locus_tag="VXN30_001284"
     CDS             6715..9081
                     /locus_tag="VXN30_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1357904.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDVIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITNTDLKEIFGK"
     gene            complement(9129..10412)
                     /locus_tag="VXN30_001285"
     CDS             complement(9129..10412)
                     /locus_tag="VXN30_001285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1357905.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3011 a         2422 c         2142 g         2978 t
ORIGIN      
        1 ataaaaatta tttataggca aaaaaatcac acgagctgtg tgattccatt atttgtcaaa
       61 atacttttta gtttcagcaa taacgactgg cgacaagacc atgagggcaa tcaagtttgg
      121 cagagccatc aaggcgttaa cgatatctgc gataatccag accatatcca actcgataaa
      181 tcctcctaac aagaccatga gcacaaaaaa cacacggtag agccagataa agcgagcccc
      241 aaagaggaac tcaaaacagc gttctccgta atagttccaa cctagaatcg ttgtaaaggc
      301 aaaaagtaca aggaagatgg tcaagagggc aggcccaaag tgtgaaaaga ctgttgagaa
      361 agctgactga gtcaaggcaa ccccattcaa gtcaccactc caaactccag ttaccaagat
      421 ggtcaaacca gttagagtac aaatgatgag ggtatcaata aaggttcctg tcatggaaat
      481 caaaccttgc tctactggtt catttgtctt ggcagctgca gctgcaatag gagcagaacc
      541 cagaccagat tcgtttgaga acacaccacg cgccacacca ttttgaatag ccatccgaac
      601 gctagcacca gcaaatccac ctaccgcagc aaggggacta aaagctgagg taaagactaa
      661 agcgattgtg ccagggattt ttccgatatt aaagaaaata actgtaagag ttcctaagat
      721 ataaatgagg gccataaaag gaacaacagt agttgaaacc ttagaaatag acttgagtcc
      781 accaaagact gcaatcgcta caaagacaga caagacgaga gctgtgatgg ctggcgaaat
      841 cgtcgttgta ttttggatag attctgtaat cgagttgact tgggtgaagg ttccgattcc
      901 caagagagca accaatactc ctgctactgc aaacaaaaca gcaagtggtc gccacttttc
      961 tcccatccct agaaggatat agtgcatggg acctcccgct actgcaccat ggtcgtcctt
     1021 ggtgcggtat ttgatggcca agagtccttc cgcatacttg gtagccattc caaagaaagc
     1081 cgccatccac atccaaaata gagctcctgg tccaccaacc ttgatagccg tcgcaactcc
     1141 tatgatattt cctgttccaa cagttgatgc caaggctgta cacagagctg caaaactgga
     1201 tacatcacca tgtcccttat cctggataaa aataagctga aaggccttgg gcagacgcaa
     1261 aacctgcaag agtcctagcc gcatggttag gtaaatccct gttccgacca ataaaatcaa
     1321 gaggggcggt ccccaagcaa aagcatcgat tgatttaagc aattctaaca tttccttctc
     1381 ctatcgtttc aaccccaaaa gaaagagcac atgcaagata catgtactct ggaatgctta
     1441 gataaatgct aaaaagcggt ctatcctagc tctgtccttt tacctgagag tttgagcagt
     1501 tgcctgcctt gccccttcgg tgcctttacg gtctctccag agttccgtcc atttacagtc
     1561 atggaaaatc aaacgattcc ccacttctat taaacttcat tcggtgttgg tatttaattg
     1621 attctaattt tacaaaaaat gttggctttt gtcaatgtgt ttattagtaa aaattagttc
     1681 aacagttttt actttataaa gtccagaata ctgctatcct ttaaaagtga caatagtcgc
     1741 accactgcct ccagcatttt gtggggcata gccgaaactc ttgacatgtt tgtttctttg
     1801 caagtatttg gtaactcctt cacggatgac tcctgttccg ataccatgga tgatatcaac
     1861 ttgagccata ttgttaagca aggcttggtc gatgaaggta tctagctcat tcatggcttc
     1921 ttcatagcgc ttgcctcgaa gatccagtct agcttgaggt cctcgcccag aagttcgttt
     1981 cacaacattg acctgtttct tcttgactgg tttttcttgc tgggcttgaa caagatcaaa
     2041 ctctttctct tccaaggtca tcttaatcaa gccaacttgg gcttcccagc gaccgtcctt
     2101 gagttgactg gtcaaggtac cacgctgacc ataactgaga accacgatat catctcccac
     2161 ctttggagct cgttttttct tggccttttg aaggacctta tttttagaca agtccacttt
     2221 ttcaggagcc aattttttca acttggcctt ggcttcaatg atttcgtggg gcttgagttg
     2281 ggatttactg tggagatttt tgagaatctg gtcactttca cttagggcca tatccacaat
     2341 ctcagcagcc tgttcacgcg ccttgttaag ctcggtttcc ttttcacgat taagctcgtt
     2401 gtagagtttt tttagcgcac ggttcatctt gagattttct tgctccacct cacggatatt
     2461 gtccaaacgt ttgcggcttt ccagcgtctg ctcttctaat tgctcaatga tacgattgac
     2521 gtcattgtcc tgatcgatct gctgactggc atctcctacg ataacttcag ataggcctag
     2581 acgtttggca atttcaaagg cattacttcg gccaggaaca ccctgcataa agcgataggt
     2641 cgggcgaaga gttgcagtat caaactccat actggcattt tgcacaaagg ctgtctcaat
     2701 accgtaggcc ttgagttctg gatagtgggt cgtcgccatg gtcttgattt gacgcaggcg
     2761 aaggtcctcc agaatagcca tggcaagggc tgctccctct tggggatcag taccagcccc
     2821 caactcatcc aaaagtaaga gtgaatgttg gttgaccttg ccaagaatat ccacgatatt
     2881 ggtcatatgg ctagagaagg tagacaagct ctgctcaata gactgctcat ctccaatatc
     2941 agcaaagatt tcttcaaaaa taccaacacg acttcccttg tctgctaaaa tcggcaatcc
     3001 tgactgggcc atgacctgtg tcaagcccag agttttgagc atgatggtct tcccacctgt
     3061 attgggacct gtaatgacaa tagctgttaa atcttgacca aaatagacat catttgcgac
     3121 ggcatttttg accaaaggat ggcagacatg gagcagttga atctcttgat tttctgacag
     3181 ctgaggcacg actgcttgtc tttcttggat aaatcgaacc ttggcacgaa tcaagtccag
     3241 atgaccgata atccaagcgt cattagcaat ctcagccgca tgagggcgga cacgctcaga
     3301 aatttcttgg agaatgcgaa gcatttcata gcgctcatct gctcgcagac tagcaatttc
     3361 ttcgctcagt ttgactacct cacgtggttc gatatagacg gtgtttccac tagcagaaat
     3421 atcatgaacg acacctgcaa tcttattgcg gtaggtgttt ttgactggta aaacctgacg
     3481 gccatttctg ctagcaacaa ttccttctgt caacatctgc gctttttgct tgagcaagtc
     3541 ttgtaaaaca tcgcgtaccc gactctcgct atcatgtatt tttcgacgga ttcgcgccaa
     3601 ttcttcactg gcaaaatttt caatgaaacc cgcatcatta aaggcctgaa gatttccttg
     3661 taattgcgga aaatcatgta atttctcaaa ccaaagggct aattcttcca agctgacatt
     3721 ttccagattg gcgtaaaaac tttgaagttc tcggctggca agaagcacgc gtttcaagag
     3781 taggaactcc tcgatattga gagccgctcc catctccaac ctcttgcaga ctcctgcaat
     3841 ttccttagtt gagagaatag taaaatgcgg ttgctcgacg aaaagagcct gcatttcctt
     3901 catctcagca aaagcctgtt tgattttatc tgctttgaca gtcggaccca gctgtctcaa
     3961 ttgctccaag ccctgctcgg tcaacaaatg aggctcaaac aaggccttga ccttatcgaa
     4021 ctctaatgtt tctaatattt tcttattcat ctttatttcc ttgtctttga atgtctaggt
     4081 gtggtagctt tttacattct gatagattcg agtcaaatct gtaaacaaag ggctaggaaa
     4141 actcctgccc tttttatccg attaaattta tcacccagat ttgtttgagc caactggttg
     4201 ttatcggtat gctctggatg atgtgttttg cgacgatact cttttcaaga ggattttgta
     4261 taactgccat ggggatggtc gccaagattg tcaaggccat ttgcaagaca aataaggtca
     4321 ccaacatgga caagatacct gctgaaactt ggaacaactt accacccagt tttttgctag
     4381 gaatcaagtg taagagaaga ccaagcaaac gaccaatgct atagacaatc ccaaatacaa
     4441 gcaagtagcc gatgcctgca taaaagacct tatccagctg aaagagttga tccgatggga
     4501 aaaagaaagt cccctgacct tcctgcgaat ttgcataagg gagcaataaa tggaattgct
     4561 ctccaagccc cttataaaac tggccagcca taaaagccga tgccatggct gaaatcaggt
     4621 aataaacctg taagagcagg cctctccgat agccgatata aaatccccaa accaagacca
     4681 atagaagaag gaatgaaatc ataaggaatc ctcaatcttg ctctgttctt gcttacaagt
     4741 cacaagcttg tgacggagtt cttctagctc ttgctcctta tcgtcaaatt caatctcacg
     4801 gctgagctga gttgataaac agttgactgc caacaaaaga gcgattgttt catcatctgc
     4861 gctaggcatt tgttctttaa ttgcttggta tttttctgtc gcaaccttag cgatttcctc
     4921 cataaaaagg ttatcatgtt cgcttgtcaa ggttaacgat tttttcccga atgtaaattt
     4981 gaatcgattt agatttgcca taaaattcac ctcacgatat tataccaaaa ttcgctaatt
     5041 ttgtcagttt ttacaaattt tactgctttt gtggtacaat agaaactatg gcaagtatca
     5101 cactcacacc aagcgaaaag gatattcagg cttttcttga acactatcaa accagtctgg
     5161 ctcctagcaa gaatccctat atccgctact ttttgaaact acctcaagca acggtttcta
     5221 tctatacttc tggaaaaatc ttgcttcagg gtgaaggggc tgaaaaatac gccagtttct
     5281 ttggctatca agctgtagag caaaccagcg gacaaaatct tcctttaatt gggacagatg
     5341 aggtgggaaa tggttcctac tttggtgggc ttgcagttgt ggctgccttt gtcacacctg
     5401 accagcacga ctttttacga aaactcggtg tgggggattc taagactctg accgaccaaa
     5461 agatccgtca gattactcct attctcaagg aaaaaatcca gcaccaggca ctccttctct
     5521 cacccagcaa gtacaacgag gtcatcggag accgctacaa tgctgtttcg gttaaggttg
     5581 ccctccataa tcaggctatc tatctcctcc ttcaaaaagg tgttcagcct gagaaaattg
     5641 tgattgatgc ctttaccagt gctaaaaatt atgacaagta cttggctcaa gaggccaatc
     5701 gtttcagcaa gcctatcagc ttagaagaaa aggctgaggg caaatacttg gttgttgcag
     5761 tttcttccat cattgcgcgt aatctctttc tggaaaatct tgaaaatctg ggacgagaac
     5821 tgggctatca acttccaagt ggagctggaa cgacttctga taaggtggct agccagattt
     5881 tgcaagccta tggtatgcag ggactcaact tctgcgccaa attgcacttt aaaaacactg
     5941 aaaaagcgaa aaaacgctta gaaaggtaag ttatgaattc atttaaaaat ttcttaaaag
     6001 agtggggact gttcctccta attctgtcat tactagcttt aagtcgtatc tttttttgga
     6061 gcaatgttcg cgtagaagga cattccatgg atccgaccct agcggatggc gaaattctct
     6121 tcgttgtaaa gcacctccct attgaccgtt ttgatatcgt ggtggcccat gaggaagatg
     6181 gcaataagga catcgtcaag cgcgtgattg gaatgcctgg cgacaccatt cgttacgaaa
     6241 atgataaact ctacatcaat gacaaagaaa cggacgagcc ttatctagca gactatatca
     6301 aacgcttcaa ggatgacaaa ctccaaagca cttactcagg caagggcttt gaaggaaata
     6361 aaggaacttt ctttagaagt atcgctcaaa aagcccaagc cttcacagtt gatgtcaact
     6421 acaacaccaa ctttagcttt actgttccag aaggagaata ccttctcctc ggagatgacc
     6481 gcttggtttc gagcgacagc cgccacgtag gtaccttcaa agcaaaagat atcacagggg
     6541 aagctaaatt ccgcttctgg ccaatcaccc gtatcggaac attttaagaa acctaagagg
     6601 ccgagaatca ccaatctcag cctcttcttc tatcgtgaga aaatgattgg taactatcta
     6661 aacttaccag aacagaaaca cctcaactct cacctattca tgcaaaggaa ttttatggaa
     6721 gtttattttt caggaaccat tgaacggatt atttttgaaa atcccagcaa tttttatcgt
     6781 atcctcctcc tagaaatcga cgatacggac gcagaggatt ttgatgattt tgaaatcatt
     6841 gtcacaggaa ccatggctga tgtaattgag ggcgaagact atactttttg ggggcaaatt
     6901 gtccagcact ccaagtatgg agaacaactg caaatcagtc gttatgatcg cgcaaaacca
     6961 actagtaagg gcttggtcaa gtacttttca agtagccatt tcaaggggat tggtctcaag
     7021 acagctcaga aaatcgtgga tacctatggc gaaaatacca ttgacgaaat tttgcaacac
     7081 ccagaaaagt tagaaggcat cgcaggactc tctgccaaaa atcgcgaggc tttcgtctcc
     7141 actctccgtc tcaactacgg aacggagatg gttttgtcca aactagccaa ctacggcatt
     7201 cccaacaaac tagcctttca gattcaagac ttttacaagg aagaaaccct tgatgtggtt
     7261 gaaaattatc cctaccagct ggttgaggat atcaagggtt tgggctttac cattgctgac
     7321 caactagctg aggaactagg catcgaaagt caggctcctg aacgcttccg cgccggtcta
     7381 gttcacagtc tttttcaggc ctgtatggaa acaggagaca cctatgttga agcacgggat
     7441 ttgctggaac aaacccttac tctccttgag tcttcccgtc ccgtggaact agaccccagc
     7501 caagttgccc aagagctctc ctacctgatc gaagaagaca aggttcagca gattgatacc
     7561 aagatctttg acaacagcct ctttttcgct gaggaaggca tccgcagtca cttggttcgt
     7621 atccttgaaa aaggaaaaca gaagagccaa gatttagaaa ctattcaaaa gcatatcact
     7681 actgtcgagc aagaactggg gattgagtat gataacattc aaaaacaagc tatttgtgac
     7741 gttatccaga acaaggtctt tatcctgaca ggtgggcctg gtactggtaa gacaactgtt
     7801 atcaatggaa tcattgctgt ttatgccctg ttagagggac ttgacctcag gaagaaaagc
     7861 aatctgccca ttcttcttgc tgctccaact ggacgagccg ctcgccgcat gaatgaattg
     7921 acaggtttgc ctagcgcgac catacaccgc cacttgggaa tgacaggtga cgatgatacc
     7981 agtcatctgg aagattacct agatgctgac tttatcatcg tggatgaatt ctctatggtg
     8041 gatacttggc tggccaatca actcttctcc aatatctctt ctaacagtaa gatcctcatc
     8101 gtaggtgaca gtgatcagct accttctgtc agtcctggac aggttctagc ggatctgctt
     8161 catattcctt tgattcctca gactcgcttg gaaaaaattt atcgacaaag cgaagaatca
     8221 accatcgtca ccctagctag tcagattcga cagggcatct tgccagctga tttcacccaa
     8281 aaaaaagctg accgttccta ctttgaaatt gctagtggcc atattcctgc caccattgaa
     8341 aaaatcttag gtgccgccct cagaaatggt attcctgccc gtgatatcca agttctggct
     8401 cctatgtacc gagggacggc agggattgat gctatcaatc agctcatgca agacctgctc
     8461 aatcccccac aaaaagatca actcagtttt gaagctcctc agtgccacta tcgtaaggga
     8521 gacaaggtca ttcatttggt taacgatgct gaaatcaatg tctttaatgg ggatttagga
     8581 gctatcacag acctgattcc tggtaaatac accgaatcga aacaagacga gattgtcatt
     8641 gattttgatg gtaatgaagt ctcttacccc cgtaacgaat ggtacaagat tcgcttggcc
     8701 tatgccatga gtattcataa gtctcaggga agtgagttcc cagttgtcat cctaccgatt
     8761 actagtgcta gccgacgtat gctggagcga aatctcatct acacagccat tacacgtgcc
     8821 aaaagcaaac tcatcttact aggcgaatta caagccttcg actatgctac ccaacacatc
     8881 ggaactgccc gaaaaaccta tctgattgaa cgcttcagtg atctactgga gaatgttgaa
     8941 gaaaagcaac aagctgtctc tgaaacagtc acatcaagtg cctctgaaca atcctacatc
     9001 ctaaccgaag aaaactggga cagcattcca gccatgattg ggattacaaa cacagacctc
     9061 aaagagattt ttggaaaata gtacatcaga aaacccacca attcaggtgg gttttctgtt
     9121 tattaagatt attttactgt tgctgtgctt gtgatcaatt caacagcttt tttgatagtg
     9181 atatcatgtt tcaacatgtc agctgaaagc aagttttgaa cttgtgcaac ttccatgttg
     9241 tagtctgctg ccaattgctc aacttctttt tggatttctt cttctgaagc atcaaatcct
     9301 tcagctttgg caactgcttc gataacaagg ttagtcttag tacgtgactc agcttctgct
     9361 tggtattggt tgtgaaggtc ttcttgagta gttccagtga tttggaagta catgtcaggg
     9421 ttgatccctt gacgttgcaa gttcccaagg aattcattta ctgaacggtg aacttcttca
     9481 tggatcattt cttctggaag ttctacgatt tcagcatttt ctacagctgt atcaattgct
     9541 gcaccttcaa ctgcatcttt gtaagcttct tctttagcag cagccaattc tttgcggtat
     9601 ttttctttca agtcagcaag tgtttcaact tcttcatcaa tgtcttttgc aagttcatcg
     9661 tcaagagctg gaacttcttt agcttttact tcgtggatag ttgtcacgaa tttagcttct
     9721 ttacctgcaa ggtcttctgc ttggtagtct tctgggaatg ttacgataac atcaacggtt
     9781 tcgccagctg agtgacctac caattggtct tcgaaaccag ggatgaattg acctgaacca
     9841 agtccaagtg agaagttttc accttttcca ccgtcaaatt caacaccgtc gatagaacca
     9901 acgaagtcga tcacaacagt gtcgccgttt tcagcagcag cttccttgat aaccaattca
     9961 gccagattgt tgcgttcgcg ttcgatacgc tcttcaacat cagcgtcagt tacttctttt
    10021 tctacatcaa ctgatacttc aaggtttttg tagtcaccca attttacttc aggttttgta
    10081 acgacttcag cagcgataac ccagtcttga cctttttcca ttgaagttac gtcaattttt
    10141 ggttgggcaa ccacttcaag accagcttct tttacagctg cttcataagc gtttggcaaa
    10201 agagcgttca taacgtcttg gtaaagtgat tcttcaccaa attttttatc gaagatagga
    10261 cgtggaaggt gacctttacg gaaacctgga acattaagag atttcttcac tgacttgaag
    10321 acacggtcca attctggttt gatttggtct tgagagatag tgaaagtcaa gacaccacgg
    10381 tttgtttctt tgttttcaaa tgatacagac attctgtcat ttctccttaa aattttttaa
    10441 atacagtcta ttataacatt ttctataaat aaaatctaga ctaatgcaga caattataga
    10501 caacaagcct ttttcctaac taaaacactc ataaagtata gacaatttta gac
//
LOCUS       DAVLSL010000067        10536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLSL010000067 DAVLSL010000000
VERSION     DAVLSL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.068"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(33..1457)
                     /locus_tag="VXN30_001286"
     CDS             complement(33..1457)
                     /locus_tag="VXN30_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1357906.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2002..4281)
                     /gene="pulA"
                     /locus_tag="VXN30_001287"
     CDS             complement(2002..4281)
                     /gene="pulA"
                     /locus_tag="VXN30_001287"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1357907.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4393..6351)
                     /gene="ligA"
                     /locus_tag="VXN30_001288"
     CDS             complement(4393..6351)
                     /gene="ligA"
                     /locus_tag="VXN30_001288"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1357908.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6444..7622)
                     /locus_tag="VXN30_001289"
     CDS             complement(6444..7622)
                     /locus_tag="VXN30_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1357909.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7702..8565)
                     /locus_tag="VXN30_001290"
     CDS             complement(7702..8565)
                     /locus_tag="VXN30_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1357910.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8568..10469)
                     /locus_tag="VXN30_001291"
     CDS             complement(8568..10469)
                     /locus_tag="VXN30_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1357911.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3159 a         2336 c         1893 g         3148 t
ORIGIN      
        1 aaaccaggaa aacaatttcc tggttttaca cgttatttca catcaaaaac aatggatttg
       61 acatttgtca tcgcttcaat gctatattta attccttgca ctccagctcc agaacctttg
      121 acaccaagga atgggaaatt atctggacca cgctgggttt tattattaat gtggactgta
      181 cctacttcaa gtttttcagc aatttcaaag gcttttttga aatcatttgt aaagactgat
      241 gattgaaggc cgaattcaga ttcgttggca aaggcaatag cttcctctac actagccaca
      301 cgaatgattg gtaaaacagg accaaatggc tcttcccatg ccactttcat atcttttgta
      361 acttggtcaa aaagcactgg ccagagaaga ttgccctcac gtttgattgg tgtaagagcc
      421 tgagcttctt tttcttgtgc atcctcaatc aagccccaaa tgaagtcggc tgaagcattg
      481 tcaataacag gtgtaatatc agcattgtca aatggatcac caactgttaa tttagaaact
      541 tcttcctgaa gcaaagtagc taatttatct gctacacttt cgagaacaat gacacgttta
      601 atggccgtgc aacgttgtcc tgagtagcta aaggctcccg caacaatttg cttggcagca
      661 tgttccaaat ctgcatcttc tagtacaaga gctgcatctt tcccaccaag ttccaacatg
      721 ataggacgca taccagctaa acgaccaata cgttctccaa taggagttga acctgtaaag
      781 ttgatgaagt tgacttcttt gtgctcaatg atataatccc caatttctga accacgacct
      841 gtaatggtgt tgaaaacacc tgccggaatc cctgcttctt caaatgcttt agccaacaag
      901 agtccagaaa tggaaccttg tgttggtggc ttaaacatga ccacattccc tgcaatcaag
      961 gcaggtgcaa ttttagaagc agataaatta actggataat taaagggagc aatcgctagc
     1021 acgataccaa ctggttcacg acggacaaca gccagtttgt ttttacttgc tgcctcaaaa
     1081 ccaccacctt ccattgcttg tccagtgata cggagacctt cctcagcagc ataacgaatc
     1141 aagtctgctg tacgcactac ttctccaatt gctgctttaa tcccttttgc tacttctttg
     1201 gcaaggatag taccaatttc ttccttatcg cgttctaaaa tagctgctgt tttatgtaaa
     1261 taagccgcac gttcaactgc tgataaagct cgccatgctg gcagggctgc acgcgcagct
     1321 tgcatagcct catcagcttc agtctgagtc atggctggaa ctgtacccaa ttcttcttga
     1381 ttgattggtg aataaatcgt aatttcttgt tcagatgatt tccattttcc atttactaaa
     1441 ttctgatatc ttgtcaaatt cttgtcctcc tgaaaatgat taagatatat tttaccaaat
     1501 tttcagaaaa tcacaaccct ttttgcaaaa tttttgagat tattttcaca aacttgattt
     1561 ttcaaagtat actcaataaa aataaaaaaa atccactacg tcaaggcgag gctaatgtgg
     1621 tttgaagaaa ttttcgaaga gcgtgaatga gtatcatcta tagtaaaata aaaaaactga
     1681 acaatttggt tggggacagc caaaccaatt tctcacaatg tttcagaaac aagggtgtgc
     1741 tactccaatt tcagcctact ataactgtca tagattgctg aaacaaagtc taggtaaaag
     1801 tcttcataat aaaaagacct cctatcaagt gttcaaaaac tttgatagga ggtcttgttt
     1861 tgtgaaaata tttatcaaat tttctataca agtgagctgt tagccaggtt ctttctattc
     1921 tttcaatttc aatgaatgga ttttttacta atactcataa ctgggaattt gtctgtgtaa
     1981 aaatagcgag atagatggta tttataaaac actcaagaca gctagactaa tatcatttaa
     2041 aacattatct tcttttgagc gactgttggt taccaacata gctaaatttc ctgcattttc
     2101 aaattgatag ggttctgatt tagcattcac aaccaccaag aggtgttctt tgccgtgaac
     2161 ttcatagata aggtagccgc tatgttcaat cgcagaatgc acaaagacat gatggtaaat
     2221 ttcatcatag ctagagtaag aaaaggcacc agtttttgtc ttcaatcgga tgacttgacg
     2281 gataaactca atactgtctt gacgctcatt aatcaagttc cagttcactt ggttcacact
     2341 gtcaggagca ttatagctat tcatcgcacg ctctctatca tcatgggtca actcaccatt
     2401 ttcaccagtc gcaaccagtt tggtacgacc aaattcttga ccgatttcca taaaggccat
     2461 cccctgcatg agcagattca tggctgtggc agtttcgacc ttgcgcatga tttgctctga
     2521 actttggtct ggatgaaggg ttgccaataa atcgtgaaga ttgtaattgt catgggcttc
     2581 tacatagtta agcacctgat ttggatgtgt atagcttcct aattcacgac ttcctaggat
     2641 tgctttagct agaattggct ctgtcgcagc accactgaca aaacctgact tgatagcacc
     2701 ataaacttct ccccctttga cagcatcgcg ctgattgtca ttaaagaaac caatatttgg
     2761 catctggtag gcattgtcct tcttggcctt atcataaggg gcaagacctg ttcccatatc
     2821 ccatccttct ccatagagga taatgttgga gtcgatttca tccaagcttt gacgaatcat
     2881 ctgcatggtc ttgacatcat gaatccccat caagtcaaaa cggaagccgt caatattata
     2941 ttcctgcacc cagtatagaa gagaatcaat catatacttg cgaaacattt cgtgttcact
     3001 ggctgtttca tttccaacac ccgttccatt ctggaaggta ccatctggat tcatacgata
     3061 atagtaatca gggactgttg tttggaatgg tgcatcaaca actgagaagg tatggttata
     3121 gactacatcc ataatgactc caatacccgc atcgtgataa gcttgaacca tcaccttcaa
     3181 atcacgaatg acctgagctg gatcatctgg attagttgaa aaactagttt ctggcgcgtt
     3241 atagttttgt ggatcataac cccagttgta ggttacattt ccatcctcat cgtattcttt
     3301 atgacggtct gcaattggtt gcaattgaac ataattgtag cccagcttct tgatgtaatc
     3361 aaaagcagtt gactggccgt attggttaac tgttccagcc tgagcagcac ccaagaaagt
     3421 tcctcgaaga tgttcatcta cacccgatgt aggtgattta gtcaaatcac gaatatgcat
     3481 ttcacagata actgccttac atggattttc caagcgccaa gtagcctccg aaccgtgctt
     3541 aacctcgaag ttttcaactt gcttttctac atggctcaga atagctgaac gtttgccatc
     3601 agggctggtc gcgattgtat aaggatcacg tgtcagtgtt tggtgatgag ggaattggac
     3661 ttgatactga taagccttac ctaccaaatc ttcctcaaca tccaaactcc agacaccgat
     3721 tgtattgtcc ttatgattat aagagtagct attgcctctt ttcatctcaa aagtcttcca
     3781 aacgggtgca tcattagcag ctgattcata aacgacaact tgcacttctg tcgctgtagg
     3841 tgaccagagg gaaaaatgag cctgattgtc ctctacacgg caacccaatt ctccttggta
     3901 accccaatga tggtcaaaac tagcactgtt aatggcctta tcaaaggcaa aaggattttg
     3961 atttttatag aaaggactgg caatagcagg attttcagag taataaatcc tatcatcgcc
     4021 ttccaaaatc cagacctctg ttaatagggg atagtgatta aaacggatag aatattcttt
     4081 actagtttga cctgtatgaa ccacaaaatt caagctttct ataacatgtg aacttgggtg
     4141 ttcaaagcta aataaagctc caaaataatc ttctttgtag gttagcaaat caattcgttg
     4201 atcctgactt tttacaaagg agcaagtgtc atattctcca ttcttacgat ggtaatgaat
     4261 gcgcataggg tagttataca ttttttattt ttccttttta ctttgtttct atttcactaa
     4321 taaatttttg tcaatctcgt ctcaattaac agacatagtc atattctcta aactctgttt
     4381 ttaaacgatc cattacaaac tttctagcca tgcctcatct ctgacctgga taccaagttc
     4441 ttgtgctttt tgcagtttac ttccagcgtc tgcacctacc acgacgaggt cggtcttttt
     4501 agaaatacta cctgtcactt tggcacccag actttcgagt ttacttttag cttctgagcg
     4561 cttgagtcgt tccaattttc ctgtcaatac cacggtcaaa cctgacaagg ccgcatccgc
     4621 tactaccgtc tgtcctttat agtccagatt gaccccagtt tctttcaatt ctctgagcag
     4681 aatttcagag ccttctgtcg caaaataagt ctgaagactt ttggcaatca cgccacctag
     4741 actttcaata ctagccactt cctctgaatc tgcctgagac agattttcaa ttgaatggaa
     4801 atattgaagt aaaagctgac taaccttgct tccgacatga cgaattccca aaccaaataa
     4861 gagcttctcg gcagaatttt cctttgatgc ttggatagcc tgatacagtt tagcagcgga
     4921 cttttcctta accccctcta aaaggaggaa atcctcttct tgcaaacgat aaatatccgc
     4981 cacatccttg actaaattag cagcaaaaag cttctcaaca atagatggac caaggcctgt
     5041 aatattcata gcatcacgag aagcaaagtg aatcaagcct tccatgattt gagcagggca
     5101 acgcggattg atacaacgta gggccacttc atcttcaaag tgcaacaagt cagagttaca
     5161 acttggacag tttgtaggga tatctagttt ttcttcagaa acccgtttgg actctaccac
     5221 acgtaaaacg gcagggatga tgtcaccagc cttatataca atgaccgtat cgtcttttcg
     5281 gatatctttt tcagcaatat aatctacatt gtgcagggtc gcacggctaa cagtcgtacc
     5341 ggcaagttgt actggtgtta gattagcagt tggagttaca acaccggtac ggccaactgt
     5401 ccagtcaact gataagagtt gagcttcttt ttcttcggca gggaacttgt aggctactgc
     5461 ccactttgga gccttaactg taaaaccaag ttcttcttga cttgctaggt cgttgacctt
     5521 gattaccact ccatcaatat cgtaaggcag attttcccgt tcctgtccta cttcttggat
     5581 aaaattccag atttcatcta tgttttcagc caagattcgc ttaggattga ccacaaaacc
     5641 tagttgttct aggtacttca aacccttttc ttggctatca cgagttgaag ggctggcttc
     5701 ttgatagaga aacgttgcaa gattacgctt ggcaactact gctgtatcca actgacgcag
     5761 agttcctgct gccgcattac gaggattagc aaattcaggc tctccatttt cttggcgcac
     5821 ttggttaact tggtcaaagg aagcgcgtgg catgtaacat tccccacgaa ctgtgatatc
     5881 tagttcttct ggcaaagtca aagggatgtc cttaacacgc ttgaggtttt ctgtgatatt
     5941 ttcaccaatt gaaccatctc cacgtgttac cccagcaacc aaaatcccct tttcataagt
     6001 cagcgagata gataagccat cgattttcag ctcacaaata taggtcggat gagccacttc
     6061 cttacgaaca cgcgcatcaa aagcatctag ctcctcacgt gaaaaagcat cctgcaaact
     6121 ataaagagga tactgatgac tgtatttttc aaaaccatct aaaaccttgc caccaacacg
     6181 atgagtcgga ctgtctgcta gcacttgctc tggataagca gtttctaact cgaccaactc
     6241 acggtaaagg cggtcatact cactgtctgc aaccgaggga ttatcgctgg tatagtactc
     6301 agtcgcatag cgattgagca aagcgactaa ctcattcatt cttttattca taagaccatt
     6361 ttaccataaa acaagccctc ctcacaaacg agaagggcgg aaaaaacact tagtttgaaa
     6421 ttatttttga aactcaagca accttatatc aatttttcaa aatgagttcg aacataaata
     6481 aacgatatac aagacaagat gataacacca cttccaatta tcaggaaaga agagagatgt
     6541 acacttggca agactgtcat aaatcctttt gcaataggca taaatagaat agctaaggta
     6601 aaaattgtac tcagtactct tccaagaaat tcgctctcaa ccttggtttg tacttgagta
     6661 aaaaagtgaa tattaaaaat cgtcataaac aattcacaaa ctaaatttcc agaaaaggaa
     6721 agaaaagttg gaagtggtaa tcccatcata aaaactccga cacctgtcaa agccagtaaa
     6781 atcaaaagat tataaatatt agctttaatt ttactagcta gaagagcccc aatgatggaa
     6841 ccaatagccc ccatagttaa aatacttgca taggctcctt ctgacccgta aagctgattc
     6901 gaaaagggaa gtagaaattc aaaagctgca aaaaagaaat taacgctgga agctaccagc
     6961 aaaaggaaga aaatttcttg ctgatgccag atatagtgta acccatcctt gatatctacg
     7021 aaaatatctc tcccagtaaa agcctttttc tcttgaactt ttgcttcctc ttttggaagg
     7081 aaagccacta gaacaaaagc aatgaaaaaa gtcagcgagt ctagcagtag cgtcatattg
     7141 agacttgcaa actgtaaaac aaggaaggaa agaacaggag agctaacacc tacaacctgc
     7201 aaaaccagct ctaagcgaga attatagatc acaatctcat ctttctccac cacttcagtt
     7261 atgatagctt tattggctgt gcgagaaaag gcaaaagcaa tagcctgcac aatgttagca
     7321 acaatcaaag cgccaatcat ccagctatca ttccttatga aagaaatagc cagacaaaga
     7381 atcccacaaa caagatctgc cgtcattaaa atcttacgac gagaaaaacg gtctgaaata
     7441 actccgccaa agggattgac gagaatagat gtgacgagct cagaaatctg atacattcct
     7501 aaaactgtct gtcctatagt ccccatagaa gccaaccaga cactatttcc ataatcatag
     7561 agcatatttc ccattttatt gatagcccca cggctaatca actgcactgc atagcgattc
     7621 atattaaagc tcctctcaaa ttttgaaact attgtatcaa aaccgaaagg agctttttat
     7681 tttttccctt atttgggaaa attaactttt gacaaatttt tcgtagtgtt cctgataata
     7741 ggctacttgc tctggaagac ctaacacatc aaaaatatgc atggcctttt gcatctgctt
     7801 acagccttct ttacactgtc ctttttgata taaggcaaaa ccttttaaat aatggaaaac
     7861 attacgctca taaagcttaa tacctttgtc aataatcttc tctgtataag cctcaaaata
     7921 gttggcatta taaaaagaag aatgctctaa acaatgctgg taacaattga gggccaaaat
     7981 caaaactaat ctcttatggc gactaatctc ttggtaaaat tcctccctct ccataacttc
     8041 tctaccaatc cgagtgacat agtctacatc gtagaaacta tagaggttac cgaaaagaat
     8101 caactcatac atggtccatt cttctgtttt gaagagataa tctgctacct tatccaaatc
     8161 atcctgcttc atatcataag tcgcatctct ttgacaaatc agaccttgta gcaaaatcca
     8221 gttcagctca aaataaaggg aagtcgtcga actcttagac ttttcaagtt gttctctttg
     8281 aagcttttga aaacctgcaa tatcgtttga atagtaaagt gggataatct gtgccatcat
     8341 agacacatgt tcatgattat gaaaattcct tgccttatcc atgaaatttt cgattgttac
     8401 atgaatgtta tccaaaatct caaagaaacg ggagactgcc aggtcagact ccccaagctc
     8461 aaagcgagat aactgagagg tagagcagga ttcgcctgct gcttccttta aagaataatt
     8521 tccacttgtt cgaaattcac gaaatacttt tccaagatgt tccatcttta cacctgatct
     8581 gataattctt cccactcaag catagcttct tcctgacgat ggctgatttt gtccagctca
     8641 gcctgtaatt ccatgagttt gtcggcatcg tttgtttcca acatttgttc agaaatggct
     8701 tggctttgac tttctagctc ttcaatttca gcttctagac tttcgatttg tcgcatgagt
     8761 ttgcgaactt ctttttgact ttctttctgg gcctgatagt cattgactgg acttgcttcc
     8821 tttgcttgat tgctagttga agcttcctca gtctgactca tttctgctgt tgctttcttc
     8881 tcaacatagt agtcgtaatc tccaaggtag agagttgaac cattctcaga caattccaaa
     8941 acatgagttg ccacacgatt gataaagtaa cgatcatgac tgacaaaaag caaggtacca
     9001 tcaaagtcaa tcaaggcatt ttctagcact tccttactat caatatccaa gtggttggtc
     9061 ggctcatcca gaatcaaaaa gttattgttt tccatagaca atttagctaa aagcaaacga
     9121 gctttttcgc caccagatag catgccgact gattttttaa catcatctcc tgagaaaagg
     9181 aaggccccaa gacggttgcg gatttcaact tctggtgtca gtttgaaatc attccagagt
     9241 tcatccagca ccgtattact tggtgtcagc ttgctttggg tttggtcata gtaaccaacc
     9301 tcaacattag cgccaaagcg cttttcacct ttgataaaag gaatctggtc cacaatagac
     9361 ttgataaagg ttgacttgcc gataccattt ggaccaacga tagcgacagc attcatctta
     9421 cgaagatcta ggttgatagg ttgtgacaaa acttccccgt cataaccaat agcagtattt
     9481 tcaacagtca aaacaacatt gcccgacgtt ttttcagact ggaaggtcat gttggctgct
     9541 ttcttgccag cttcaggctt gtccaaacgc tccatttttt ctagttgttt acggcgagat
     9601 tgagcacgtt tggtcgttga agcacgaact agattgcgat tgacaaagtc ttccagagca
     9661 gcgatttcct tctgttgctt ttcatagttt tttgcctcag tagctagctt ttgctccttc
     9721 aactcaacaa aacgagagta attccccaca tagcgatcca aggaatactt ggtcaaatct
     9781 agcgtaattg tcgcaacctt gtccaagaaa taacggtcgt ggctgacgat aatgagggca
     9841 ccgctatagt ttaccaagta attctctagc caggagatgg tttcaatatc caagtggtta
     9901 gttggctcgt ccaagaccaa gagattgggc ttttcaagga gcattttagc tagagccaaa
     9961 cgagtatttt gaccaccaga aagctcagca attttcatct gccacataga ctcgtcaaac
    10021 ttgaatccat tcaaaatcgc tcgaatatca gcttcatagg taaagccacc tgcttggcga
    10081 aaattttcag ataagcggtc ataatctgac atcagtttat ccaaatcctc accagacttt
    10141 tcacccatct ccagctccat ctgacgcagt tgtttatctg tccgacgtaa gtcatcaaag
    10201 acatgaagca tctcatcata gatggtattt tcagactcaa aacggctatc ttgagctagg
    10261 taagacagag aaatatcttt tttcttattg atttctccac tagttggctc ctcttctcca
    10321 actaaaatct tcaaaagagt agacttacct gcaccatttt tcccaacaag ggcaatccga
    10381 tctcgttcat caacctgcag gttgatatta tcgaaaagaa cctctcctgc aaaagaacgt
    10441 tcaattttat tagcttgtaa aataatcata caagtagtat agcatgtttc cctaaggcat
    10501 tcaagataat cgtaagtctt ttagtacaac ttttat
//
LOCUS       DAVLSL010000068        10383 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLSL010000068 DAVLSL010000000
VERSION     DAVLSL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.069"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..389
                     /locus_tag="VXN30_001292"
                     /pseudo
     CDS             1..389
                     /locus_tag="VXN30_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            475..687
                     /locus_tag="VXN30_001293"
                     /pseudo
     CDS             475..687
                     /locus_tag="VXN30_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            587..1033
                     /locus_tag="VXN30_001294"
     CDS             587..1033
                     /locus_tag="VXN30_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1357912.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VXN30_001295"
     CDS             complement(1171..2475)
                     /gene="eno"
                     /locus_tag="VXN30_001295"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1357913.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2639..3085
                     /locus_tag="VXN30_001296"
     CDS             2639..3085
                     /locus_tag="VXN30_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1357914.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3082..3885)
                     /locus_tag="VXN30_001297"
     CDS             complement(3082..3885)
                     /locus_tag="VXN30_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1357915.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3894..4223)
                     /locus_tag="VXN30_001298"
     CDS             complement(3894..4223)
                     /locus_tag="VXN30_001298"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1357916.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4168..4539)
                     /locus_tag="VXN30_001299"
     CDS             complement(4168..4539)
                     /locus_tag="VXN30_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1357917.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VXN30_001300"
     CDS             complement(4598..6031)
                     /gene="glgA"
                     /locus_tag="VXN30_001300"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1357918.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYENIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VXN30_001301"
     CDS             complement(6028..7167)
                     /gene="glgD"
                     /locus_tag="VXN30_001301"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1357919.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7157..8299)
                     /locus_tag="VXN30_001302"
     CDS             complement(7157..8299)
                     /locus_tag="VXN30_001302"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1357920.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VXN30_001303"
     CDS             complement(8289..10217)
                     /gene="glgB"
                     /locus_tag="VXN30_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1357921.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3180 a         2285 c         1826 g         3092 t
ORIGIN      
        1 caccaagtaa aaggaacaaa accaagaaaa gttgatagag atagacttaa aaactatctt
       61 actgacaatc cagacgctta tttgactgaa atagcttctg aatttggctg tcatccaact
      121 accatccact atgcgctcaa agctatgggc tacactcgaa aaaaaagaac cacacctact
      181 atgaacaaga cccagaaaaa gtagccttat ttcttaagaa ttttaatagt ttaaagcacc
      241 tagcacaggt ctaacaaatg aatggtgagt taatcgctcc aatgacttac gaagagacga
      301 tgacgagcga cttttttgaa gcttggtttc agaaatttct cttaccaaca ttaaccgcac
      361 cattggttat tatattaaaa cgaagtaaga ataggacaaa tcgatcagga cagtaaaatc
      421 taattctaac aatgttttag aagtagaggt gtactattct agtttcaatc tactatatta
      481 atggacaatg caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttggg
      541 cataaacttt tacctcttcc tccctactcg cctgagtaca atcttattga gaaaacatgg
      601 gctcatatca aaaagcacct caaaagggta ttaccaagtt gcaatacctt ttatgaggct
      661 cttttgtctt gttcttgttt caattgacta tataaattgt ctaagcgaaa caaccgataa
      721 gaattggcac aaaagcgacc gtatttttgt taccaataca ggaaaaccag ttcatagttc
      781 tatcttgagc aagtctctcc agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca
      841 tctgtcccct cacatcttca gacacaccac tattagcatc ttatcagaaa ataaaattcc
      901 tttaaaaaca atcacggaca gggttggtca ttccgactct gaagtcacta cttccatcta
      961 cacccacgtc acaaagaaca tgaaagatga agcaatcaat gtactggata aagttatgaa
     1021 aaagattttt taaaaagttt tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg
     1081 gataaaatcc gaggggctag aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg
     1141 gttcgggata aaatagttca ctgaactatt ttatttttta aggttgtaga atgatttcaa
     1201 tccacgatat tcagctactt caccaagttg gtcttcgata cgaagcaatt ggttgtattt
     1261 agcgatgcgg tctgtacgtg aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac
     1321 tgcaatatca gcgattgttg aatcttcagt ttcacctgaa cggtgtgata caacagcagt
     1381 gtaaccagct tctttagcca tttcgatagc ttcaaaagtt tcagtaagag taccgatttg
     1441 gttaactttg ataaggattg agttagcagc accttcttgg ataccacgtg caaggtagtc
     1501 agtgtttgtt acgaagaagt cgtcaccaac aagttgtact ttcttaccaa gacgttcagt
     1561 aagagctttc caaccatccc agtcgttttc atccatacca tcttcaatag tgatgattgg
     1621 gtatttgtta accaattctt caaggtagtc gatttgttct gcagatgtac gaacagcagc
     1681 accttcacct tcaaatttag tgtagtcgta aactttacgt tctttatcgt agaattctga
     1741 tgaagcacag tcaaatccga taaatacgtc tttacctggt acatatccag cagcttcaat
     1801 cgcagcaagg atagtttcaa caccatcttc agttccttcg aaacgaggag cgaatccacc
     1861 ttcgtcacct acggcagttt ccaaaccacg tgatttaagg attttcttaa gagcgtggaa
     1921 gatttcagca ccgtaacgaa gggcttcttt aaatgttggc gcaccaactg gcaagatcat
     1981 gaactcttgg aaagcgattg gagcgtcaga gtgagaacca ccgttgatga tgttcatcat
     2041 tggagttgga agaactttag tgttgaatcc accaaggtag ctgtaaagtg ggatttcaag
     2101 gtagtcagca gcagcacgag ctacagcgat agacacaccg aggattgcat tcgcacccaa
     2161 tttacctttg ttaggagtac cgtcaagtgc aatcatagca cggtcaatag cttgttgatc
     2221 acgtacatcg tagccaatga tagcttcagc aatgatgttg tttacgttgt caacagcttt
     2281 ttgtgtacca agaccaccgt aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg
     2341 ttcaccagta gaagctcctg atggaaccat accacgtccg aaagcacctg attcagtgta
     2401 aacttctact tcaagtgttg ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc
     2461 agtaataatt gacatttttt actctcctta tgagttaaat tttttacacc tctataatac
     2521 cttaaaaccc ctcctttttc aagaaaaaac gttatctttg tgcaactttt ccttaacttt
     2581 ataaagtaat cgctttcttt tgtctgtttt attctaactt ttatgatata ctgttttcat
     2641 gacagattta tcaaaacaat tacttgaaaa agctcatggt gggttaaaaa taaatccgga
     2701 tgagcaaaga cgctatcttg gtacttttga ggaaagagtt cttggatatg tagatattga
     2761 cacagcaaat agccctcagt tagaaaaagg ctttttattt attttagaaa accttcagga
     2821 aaaagcaaag ccactatttg tgaagatttc accaactatc gaatttgata agcaagtttt
     2881 ctacttaaaa gaagcaaaag aaactgatag tcaagccacc atagtatctg aagagcatat
     2941 tacttctcct tttggcctgg ttattcatag caatgcacca gttcaagtag aagaaaaaga
     3001 ccttcgactt gcttttccaa aactttggga agttaaaaag gaagaaccag ccaaaacatc
     3061 cttatggaag aaatggttta gctaaatctt gcacatattt aataagtgcc caatattggc
     3121 agccgtgcgc tccagataga gactggcatt tttcaaacta tcttctaaag gttcactttt
     3181 ctccaaaata gaaaagacag cttggatatt ttcaaatggt aggggaggta aatcttcagc
     3241 aagactaccg caaatagcaa taacaggaac tccaacaggg gttctttttg caacacctat
     3301 aggcgctttc ccagcaaagc tttgactatc aagtcttcct tctccaacaa caaccaagtc
     3361 agcatctgaa actttcttat caaagttgat taagtccaag caggtatcaa ttccagacac
     3421 gatacttgcc tgagcaaagg cacacaaacc agcagcaatc cccagtcctc catcattact
     3481 ggccgtgcca ccaacaccga tataaatatc tttaatccct ttagaaatga gatggagaat
     3541 caactctcca ataccacaag tttggatttg aagtggattt cgtttctcta gcggaatttt
     3601 tccaagacca accaagtcag ctacttcaaa tagtgccagt tccccttttt gaaaatagcg
     3661 catggcttct ttttgtccaa aagggtctgt cacttggatc catttttctt ttaggtcaag
     3721 agaatgtcgg atagcatcta cagtaccttc tcccccatca ccaacagggc agaggagaca
     3781 ttctacatct gctatcgatt gttggaagcc tctttttatt gcttcagcta cctgttgagc
     3841 tgtcaagctt tccttaaacg aatccggtgc aattacaatc ttcatatttt ccctcattct
     3901 aaacagtcaa tcaaaggaag aacttctaaa aaatccctct tgtcaacatg atgtggtatt
     3961 tcttttttga gcatttcttt ggaacaaaag gcgattccta actccgccga cttcaacatt
     4021 aatagattat taaccccatc accgattgcc accgttcttt ctttagaaag ttttagtttc
     4081 tttctccatt tttccagagt ttcttttttg acctggggac ttataatttg tccaactaat
     4141 tttcctgtta aaagaccttc tttgacttca agctggttgg cagtgaaata ggcaatacca
     4201 agggattttg ctaatctctc caactattgg tgtaaatcca ccagacacca gaccaactag
     4261 gatgccattc ttttggagaa tagagatgaa ctctgggaca tttagcgata gatgaattga
     4321 gttgaagacg ttatcaaaga ccaaaatagg aagaccttcc aacaaggaca ctctttttct
     4381 taaactgctt tcaaagacca actctcctcg cattgctcga cttgtaatct gcgaaatttc
     4441 cgcctcatga cctgcctctc tccctaaaag atcaatcact tcttctagga ttaaggttcc
     4501 atctacatcc aaaacacaca agccttttac ttgagacatc agttctcctc tctaaacagc
     4561 ctaaaaatcg tatgaagtca tcatacgatt ttatctatta attaactaaa ctatggtaca
     4621 agtcaaggta tgacttgcag gctgtatccc atgagaagtc actctccata gcttgttttt
     4681 gtaggtttct ccaaatgtct ggatggtttc tatacaagtc caatgctgtt tggaaagtcc
     4741 aatttaacca ataaggagat agattgtcaa agctaaagcc agtaccgctt ccttcgattg
     4801 gattgaaagc gcgaactgta tctcgcaagc ctccaacttc atggaccaat ggcaaggttc
     4861 cataacgcat agccatcatt tgagacaagc cacacggttc aaaacgactt ggcatgagga
     4921 agaggtcaca agcagcgtag atttcctgag caagtttgac atcaaaagtg atatttgctg
     4981 atagcttgtc tgggtaaatc tgagcaaacc atgagaaagc tccttcaaag gctggatcgc
     5041 cagttcccaa aagaacaatc tgaacatctt cttgcaagat atggtgaaga ctttcgacca
     5101 ccacatcaaa acctttttga cgtgtcaaac gagaaacaat tcccaccagt ggaacgtctg
     5161 ctctaacagg caagccaact ctttcttgca attttgcctt atttttggct ttcccagaca
     5221 aatcttcctg attgaaatga tagtctaaaa gagcatccgt ctgaggatta taaagatcag
     5281 catcaatccc attcacgata ccagatactt taccagactc cattttaaga atctgatcca
     5341 aattacatcc aaactgacta gtcataattt catgagcata gctaggtgaa acggttgaaa
     5401 cacggttcgc atagagaata cctgccttca tccagttcag acagttgttc catcgaaggg
     5461 tgccatcagc gtaacgttca aggccaactc caaacaaatc acccaacatt ccttctgaaa
     5521 attgtccttg gaattctaaa ttatgaatgg ttaaaactgt ttcaatgttc tcataggctt
     5581 gaatccaaca gtatttttcc ttcaacaaga aaggaatcat agctgtatgg tagtcatgaa
     5641 catggagaag atcaggaata aagtcaatcc tttccatagc ctcaatggca gccagttgga
     5701 aaaaggcaaa gcgttctccg tcatcaaaat caccgtaaac atgaccacgg aagaaataat
     5761 attgattgtc aataaagtag aaggttacac catttaatac tgttttctta attccacaat
     5821 actgtctacg ccaaccaacg ctcacctcaa aatgaagcac atcttcaatc tgatttccaa
     5881 atttagcctc taccatatca tagtagggta aaatcactgc aacttcgtgc ccagctttta
     5941 ccagtgattt tggaagagcg ccaatgacgt ctcccaaacc acctgttttt gaaaagggtg
     6001 caccctctgc tgctacaaat aaaattttca tgaatgaata tcctctgtta ctttagcacc
     6061 tttcttaacc acaactggat gttctgcagt tcctcgaatc acaacaccaa gctcaacttc
     6121 aacccctttg tccaagatag catattcgac ctgagcccct tctccaataa caacacgagg
     6181 gaagagcagg ctatctttaa ccaagctatc cttatggaca tgaatattac gtgatagaac
     6241 agaattagct acttgacctt caataatact accagaggca aactgagaag tgcttacctt
     6301 agatgtatta gcatagtaag ttggctcttc gtttttgacc tttgtataaa tcttttggtt
     6361 tggtgagaaa agagaataga atttttgtga ttcaagcata tcgatattcg cttgataata
     6421 agatttaaca gagtgaatat tggctagata gcccgtgtac tcgtaggcaa aagctccctc
     6481 ttttacagcc aaatcccgta aaacatagcg caatttctct ggatgttctt ttttagcttc
     6541 ttcttccaag tgttcaatca accaaggtgt atcaacgaca aagatatctg tagacatatt
     6601 gaacgtttca gctgttgact tgctatcaaa gagtttatga gaaagaacat ggtctgtttc
     6661 atctacatcc aagattgcat ttacttctga aatatctttc ttagctagtt ttttataaac
     6721 tacagtgata ggctcttttg ttgtactatg taggtggaaa acttggttca aatcaatgtt
     6781 aataagaaca tcgcagttga gggcaaccgt ttggtttgag ccagaacgtt tcaaataagt
     6841 aagaagctgt tggtagtatt cttttccaac tgtactactt tctacacggg tattgtaaat
     6901 tcctagatag taatggctaa gaagggttga taagccccac tcgcgccctg aacgaatatg
     6961 gtcaaagact gagctgatat tatcctgctg gaaaatacca aagacactac gaacacctgc
     7021 attagcaagg cttgaaagtg ggaagtcaat caaacgatat ttcccaccaa atggcaaact
     7081 tgctactgga cggtggtccg tcaatgtcga catatcgtga aaaccaactg tatttcctaa
     7141 aatggcagaa tatttatcaa tcttcatctg ttgctacccc cactacttca ttatatccta
     7201 caacttgtac ttcatctgtt ccatcaattt cgacaccgtc agaaataatc gcaccttcac
     7261 caataatggc acgtttaatc ttagctcctt gaccaatgat agctccactc atgataactg
     7321 aatcaaggac ttccgctcct tcgcgaactt gcgcgcctgt tgaaaggata gaatgtttaa
     7381 cagttccatc aacgaaacat ccgtctacaa ctaatgagtc ttccacatga gcatttgccc
     7441 cgaggaagtt tggtggtgaa atcaagtttc ttgagtaaat cttccattga cggttacgac
     7501 tatccaaggc attttctgga gaaatatact ccatgttcgc ttcccaaagt gactcaatag
     7561 taccaacatc tttccaataa ccactaaatt cataagcata aacactttca cctgactcaa
     7621 ggtaatttgg aatgacattt ttaccaaagt ctgacatgcc aaccttgctc ttttcagcag
     7681 cgactaacat attacgaagg cgttgccaat caaaaatgta gattcccata gaagcttttg
     7741 tagatttagg ttgagctggt ttttcttcaa attcaacaat acgattgtta gcatctgtgt
     7801 tcatgatacc aaaacggctt gcttctttaa gagggacgtc taaaactgct actgtcaagc
     7861 tggcattatt atccttatga gactggagca tatcatcata gtccattttg tagatgtgat
     7921 ccccagacaa aatcaagaca tactcaggat tgacactgtc gatatagtcg atattttggt
     7981 aaatagcgtg actagtcccc tcaaaccaac gatttccttc acttgcagaa taaggttgaa
     8041 gaatagagac acctgaatta ataccgtcta gtccccagct tgaaccattc ccaatatggt
     8101 tgttgagagc aagtggttga tactgtgtaa cgaccccaac attatgaatc cctgagttgg
     8161 cacagtttga tagggcaaag tcaatgatac ggtagcgccc accaaattgc acagctggtt
     8221 ttgcgatgct ttgagtgagt ttaccgagac gagttccttg cccaccagca agaatcaaag
     8281 ctaacatttc atttttcatt ttctactcct ttttggtttt tatttgtgac ggttttagta
     8341 gatttcaagc gacgtttgat tttccataca cttgctccca tagccggtag ggtaaaggtt
     8401 aaggtctgct cataatcttt ccatagtcct tcttgcgttt gaacagtttg attatgttct
     8461 ttccaaacgc ctccccactc ttccaactca gtattccata cttcttcgta aattcctgca
     8521 acgggtagtc cgattgtaaa atctttccgc tcaacaggta ccatgttaaa gatacagact
     8581 aacatttctc cctttttacc cttacgaata aaggaaagaa cactctggtc tcgattatcc
     8641 gcatcaatga tttcaatacc atcatagctg gtatcgattt cccacagaca gcgatgatct
     8701 ttgtaaaact ggtttagctg agaagtgaaa tacttcatct tagcattcat tgggtcttct
     8761 aggttagacc attccaactg ttcttcagat ttccattcta ggaattgacc gtattcgcta
     8821 cccatgaaga gcaatttctt accaggatga caaatttggt acgtatagag attgcgcaag
     8881 cctgcgaatt gattgtaacg atctccccac atcttatgca tcatactctt cttgccatga
     8941 accacttcat cgtgcgagaa tggcaagaga taattctcct tgaaaacata cataaagctg
     9001 aaagtcacca ggttaaagtc atatttacga tagatcggat cttcttcgta gaaacggagg
     9061 atatcattca tccagcccat gttccatttg tagtcaaatc ctagaccacc aatctctttc
     9121 attcccgtaa tcttggtcgc agacgaactt tcttctgcaa tcatcatcac atctggatat
     9181 tctaacttaa taacctcatt caagcgctga aggaaataat aaccttcata gttgagattt
     9241 ccgccatctt tattaggtgt ccatggagca tcatcatagt ccaaatagag catgttgcta
     9301 acagcatcca cacgaatacc atccaaatga tagacatcaa tccaatgctt aatgcaagaa
     9361 attaagaagg actggacttc attttttcca aggtcaaaat taagggcacc ccaaccatgg
     9421 ttatgagcct tattatggtc ttggtattca aaagtcggtg tcccatcata ataggctaag
     9481 gcatcatcgt tgatggtaaa gtgacctggt acccagtcca caataacccc aatattatgg
     9541 gtatgacact cctcgacaaa atcttgaaac tcctctggtc ggccataagc atgctctaaa
     9601 gcgaagtaac ccataagctg atacccccaa ctcaagccca aaggatggga catcaagggc
     9661 ataaactcaa tatgagtata gttcatttca acgagataag gaatgagttc atccttgagc
     9721 tgggcaaaac tataaggact gccatcagaa tttcttttcc atgatccagc gtgaacttca
     9781 taaatattga caggacgctc ttcaaagccc caacgttttc ttcgtgccag ccaaagtcca
     9841 tccttccatt tcttctcacg aagctctgtt aagattgccc ctgttcctgg acgagcctca
     9901 tacctgacag caaaagggtc aatcttcatc agttgatgac cattttgacg tgtgacatga
     9961 tatttgtaaa tatgcccttc ttgagccata ttggtaaaga cttcccagac cccaaaatca
    10021 tttcttacca ttggaatctg attttcaatc cagttggtaa aatcaccaac caagtgaaca
    10081 gcctgagcat taggtgccca aacacggaag gtatagccat gctctccatt tagttcttcc
    10141 ctatgtgctc ctagataatg ttggagataa aaattttcac ccgtcataaa ggtttttaat
    10201 gcttctctat tatccatata ctcctcttct cctgtaagcg ttttctatgt ttttattata
    10261 ctaccttttt agagaagatt caagtaaatt actatacttc tttaattatt ttgaaaatct
    10321 acaacaagtt cacttactcg ttcaattgta aatcaatatt ttttcaaaaa attgcgaaaa
    10381 ctc
//
LOCUS       DAVLSL010000069        10342 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLSL010000069 DAVLSL010000000
VERSION     DAVLSL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.070"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(47..463)
                     /locus_tag="VXN30_001304"
     CDS             complement(47..463)
                     /locus_tag="VXN30_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357922.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(475..1170)
                     /locus_tag="VXN30_001305"
     CDS             complement(475..1170)
                     /locus_tag="VXN30_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1357923.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(1364..3865)
                     /locus_tag="VXN30_001306"
     CDS             complement(1364..3865)
                     /locus_tag="VXN30_001306"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1357924.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(4320..5408)
                     /locus_tag="VXN30_001307"
     CDS             complement(4320..5408)
                     /locus_tag="VXN30_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1357925.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(5426..6094)
                     /locus_tag="VXN30_001308"
     CDS             complement(5426..6094)
                     /locus_tag="VXN30_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1357926.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(6113..8551)
                     /locus_tag="VXN30_001309"
     CDS             complement(6113..8551)
                     /locus_tag="VXN30_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1357927.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(8702..10000)
                     /locus_tag="VXN30_001310"
     CDS             complement(8702..10000)
                     /locus_tag="VXN30_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1357928.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSMTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFVAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLAMSTMVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(10017..10325)
                     /locus_tag="VXN30_001311"
     CDS             complement(10017..10325)
                     /locus_tag="VXN30_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1357929.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
BASE COUNT         3163 a         2279 c         1716 g         3184 t
ORIGIN      
        1 aatactagac catccttaaa ttttgcttaa gaaaaaacaa gttttgttat tttttacgat
       61 tcatccaagt catccctata caattatagg tggataagat ttcaaagccc atagaacgat
      121 agaagcccaa ggttctttct gtctcttctg tcaccagctg gacttgatag gcatctttgt
      181 aatcctctaa agcctctttc atcaaggcac taccaattcc ttgacgctga tagattggta
      241 aaacgattaa atcctgaacc aatactgatg agaatccatc tccaaccaaa cgaatcaagc
      301 ccaccacagc atcaccatca agtgccagat aaattactaa tgaatgagat aaggcctgct
      361 ccagcatctc tggttgatgg gtataatttg tccaaccgac agcctgatag agatgtaaaa
      421 catcctctag cttgacaatt tcttgctttt taatagtaat catctcaaca cctcttaaag
      481 ttctctcaag ctcttgtact gctgtccatt tttatcaaaa ttttcaggac gcaaccatgt
      541 ttccaaacga tctttgactt gaggccagtc cttatcaatc atagacaacc aatccgtatc
      601 tctcgtacgt cccttataaa ccactgcctg acggaaggtt ccttcataaa taaagctcaa
      661 acgctccgca gctcgtcttg atggcagatt taaggcatcg catttccact cgtagcgacg
      721 atagtttagc tcctcaaaaa catagcgagc taggagatac tgagcttcag ttcctatccg
      781 tgtacccctt agctctggag aaaaagtgac tgctccgact tctattactc ggttattctg
      841 gtcaatgcgc atgagagaaa aagatcccaa agccttacca gtcgccttgt ctatgattgc
      901 atagtaaaaa cggtccttac gagccaacat ctgatgtaaa aggctaacca gctcccccat
      961 atctgctact ggctcctgaa aaaggtaggt ccacatctcc cgaggagtat ccgggccata
     1021 aacagctagc aaatcctccg catgcttttc cactgaaaga gcctctatcc tagcataacg
     1081 cccttctaag aaatcaatag aaggcagttc acctggtgta taaccttcca ttgactcccc
     1141 aatcatttga ccatattcat ttactggcat agctttctcc ttttttcact tttcaaactt
     1201 tatagttgcc catagtatac catactttca taagaagatt caaaaaatcc tccagattct
     1261 actctgaagg attcctacct tatttcacaa cgatattaac gagtttagtt ggctcaaaca
     1321 gtttgggaaa ctgtttgagg ttggaaatca gcaaataaat tggttatttt acaacaatat
     1381 taaccaattt atttggtaca ctaatcactt tcacgatttc cttaccgtca atttctgctt
     1441 tgactttttc atccgctaga gcaatttctt gcaattcttc acgtgatagg tctttagcga
     1501 ccatgagttt ggcacggact ttacctttga tttggacaac gatttcgatt tcgtcttcaa
     1561 ccagtttact ttcgtcccaa gttggccaag ctacataaga gattgactca cctgtttctg
     1621 cgactgtttg ccagagttct tctgccaagt gaggtgcaaa tggtgcaatc aattgaataa
     1681 agcctttggc atagtcaaca taaagcttat cttccttgtt agcagcattg acaaagacca
     1741 taagttgggc aatagctgtg ttgaatttga gagactcaat ttgctcagta acagctttga
     1801 ctgtttcgtt gtaagccttg tcaagagcac cattgttttc cgcaaggatt tctttacttg
     1861 taatcaaacg gtaaactcgg tcaaggaact tacggcttcc ttccaaacct tcttctgacc
     1921 aagcaatcga agcatcgagt ggtcccataa acatttcata aacacgaagg gtatcggcac
     1981 cgtattgttc caccacatca tctgggttaa caacgttctt gagcgattta gacatcttgg
     2041 ctggcgcttg ctccaactct tcccctgttt ctatatgaaa gaaggaacca tcacgttttt
     2101 caaccttgtc ggttgccaca agagcaccac ggtggtcacg gtagcttgtt cccaaaatca
     2161 tcccttggtt aaagagtttt tggaatggtt ccttagtcgg aacaacaccg aggtcataga
     2221 ggaatttatg ccagaaacga gcataaagca agtgaagtac agcatgttcc gcaccaccca
     2281 cgtagatatc tactggcaac cattgtttga ggaggtcctc atcagccaat ttctcagtat
     2341 tgtgcgggtc aatatagcgg aggtagtacc agcttgaacc agcccattgt ggcatggtgt
     2401 tagtttcacg acgacctttg acaccatctt cacgagtcac ttcaagccaa tctgtcaagt
     2461 tagctagtgg actttcacca gtacctgaag gacggatatc cttggttaca ggcaagacaa
     2521 gcggcaattc agtttcagga acagctgttg aagttccatc ttcccaatga atgattggaa
     2581 ttggctcacc ccagtaacgt tgacggctaa agagccagtc gcggaggcgg taggtaacct
     2641 tctcctgacc acagcctttt tcttccaagc aagccacaat cttggcaata gcgtcttctt
     2701 tgttcaatcc atctaggaag tctgaattga catgcaggcc atcctctgtg taggcagctt
     2761 cttcgacatt tccaccttca agtacttcga cgattggaag gtcaaattgt ttggcaaatt
     2821 cccagtcacg ttggtcgtgg gcaggcacag ccataaccgc acctgttcca taactagcaa
     2881 gaacatagtc cgcaatccag attggcattt ccttaccatt gacagggttg atggcatagg
     2941 cacctgtcca aacaccagtt ttttccttag caaggtctgt acgaaccaag tcagacttaa
     3001 ggctggcttg gtgtttatag tctgctacag cttctgcttg ctctgaactt gtgatagcgt
     3061 ctactaattc atgttcagga gccaagacag tgaaagtcgc accgaaaagt gtgtccggac
     3121 gagtagtaaa gactgtaaat tccttgtctg ttccttttac tttgaaagtt acattggcac
     3181 cagttgattt accaatccag ttgcgttgca tatccttgat agactctgac caatctagtt
     3241 catctaagtc attgagcaag cgctctgcgt aagccgtgat tttgagcatc cattggcgca
     3301 ttggtttgcg gacaactgga tagcctccac gctcagaagt tccgtcagga agcacttctt
     3361 cattggcaat ggcagttccc aattcctcaa cccagtttac tggcacttca gcttcatagg
     3421 ccaagccttt ttcgtaaagc ttggtgaaaa tccattgagt ccacttgtag tagtttggat
     3481 ctgttgtgtt gacttcacga tcccagtcat aagaaaatcc aagcgcattg atttgacgtt
     3541 tgaagttggc aatgttttcc gctgtaaatt ctgctgggtc attaccagta tccatagcgt
     3601 attgctctgc aggcaaacca aaagcatccc aacccattgg gtgaagaaca ttgtagcctt
     3661 gcgcacgttt gtaacgactg aggatatcgg ttgcagtata accttctggg tgtcctacgt
     3721 gcagaccagc tccagacgga taagggaaca tatcaagcgc ataaaactta ggttttgatg
     3781 catctgttcc tgtcttaaat gtatgatgtt ctgcccagta gccctgccac ttaggctcaa
     3841 tttctttatg attataaaaa ctcatggtct ctctccaatt ttgtgatgtt actattatac
     3901 catttttgag ggaatttgaa atgtgtttaa aggaaaaact gtatactgta tctcattaat
     3961 tttgatgact atgaattgct ctatctccca ctttttatcc cattgttatg cagtaaatca
     4021 aggagtaagt caagaagctt atctcgtata gcacaaaata cttattttag accgaagata
     4081 aaactggaat gctaattaat gtattcacaa taaatagccg acaacctatg tcgctttttg
     4141 atttatgaag aacgtatagt gcactgaatc tgaaatagta cgaaacaatt gctaaaacat
     4201 tgttagaaat taattttact ttcccaatcg atttgttctc atcttatttc aatctgctat
     4261 agaattcaat acgaggatca ataaaagact accatttgga aaacagtagt ccttattgtt
     4321 tattttgaat ttacataggc atctacagcg ataatagctt gagcaacatc tgaaggcgaa
     4381 atcttaaacg gcatctgatg aattgtctca ccctccatag ttgcttgttt accaactttt
     4441 attaaatcat catatccaac ttgatccaaa tgcatttctt ttagagttgt tggcatacca
     4501 atttttttgt aaaactcaat atacttatca agttcttctt taggtctatt ttccaataat
     4561 agttgtacta aagttccata agctactttt tcaccatgtg ttaaatgatg aatgtcacct
     4621 gtcaatgcag taaaaccatt atgaattgca tgcgccgcag ctaatcctcc actttcaaaa
     4681 cctagaccac tcaataaagt attagcttca acaatatttt ctaatgctgg tgtcaccact
     4741 ttagcttcac aagctgccat agcctgtaaa ccatctgcaa acagcgtttc ttcacatttc
     4801 ttcgcaattg caactccagc caatgtttgc tgttgtccca acatagtttt tccatttgcc
     4861 tgcataaccg cacgcgcctc aacccaagtt gctaaaccat ctgcaatacc agacgctaat
     4921 aaacgcttag gggcttgtga aataactttt gtatcaacca aaactaaatc tggattttta
     4981 gaataaaata gataatgatc aaatgcacct tcatctgtat aaataacaga taaagcagat
     5041 acaggtgcgt cggtcgatgc aattgttgga gcaataataa caggcttttc aatcaaatct
     5101 gcaatagctt ttgcgctatc aatcgtcttt cccccaccaa gaccgataat actatcacaa
     5161 ttttctttct cagccaaggc aacaactcga ttgatttcat tgtcagaagc ttcaccatta
     5221 aatagcgcca gaacaatatg gaaaccatac ctatgtagat aatcttcaaa tcgttttcca
     5281 acaatatcat aaaccaactg atcgcataat agaatagggc aatttcccaa atccaaaatt
     5341 gatttggcat tttcaaacaa ggcattttcc ccctgaatat atctagaagg actagcaaaa
     5401 attctcatat gtatttcctc tctatctaaa tggaacgact attttgaata acaaaccagt
     5461 cgtcagaaaa gtcatcaacc gccttttgga tagatggcat ggcgaaagct gattcaaaaa
     5521 catctgctcc tgctgtaaca gcatgcgcac ctgcagctaa agcattattt acttgtgcta
     5581 catttttaaa ggatgcagct aaaatcttac taggagagtt ctgtctatcg atagcaagag
     5641 ctaattgacg aatgacagaa tttgaatcaa tgttcagatt ttccattcta ttataatatg
     5701 gagctaggta atccgctcct gcttcgatag ctaataatcc ctgaataact gtataaatag
     5761 ctgttgcagt gatatggtag ccctcttttt ttagcacctt tattgcacgt aatccagctg
     5821 gagtaacagg tactttgata aatatatcat ctcctgcttg tcttcgaatt tcatgagcat
     5881 cctttaagat gccttcaaaa tcttgagaaa tcacctgaac atgaatagag ggtgtagagc
     5941 caatcaattc tcttacatct ttgattcgtt caaaaaaatt aatagaaccc tctctttttg
     6001 caatagtggg atttgaagtt accccagcta gcggcaaaat ttcagaccac tttttaatct
     6061 catctaaatt taatgtgtca agcataaatt ccataaaaag aacctcttta ttttacaaag
     6121 tatgctcagt acgtcctata atgtcatctt gggttgcctt agaaagaaca ttgaagaatg
     6181 cagagtatcc tgcaacacga acaattaagt ctctgtgttt ttcaggatgt ttctgagcgt
     6241 caatcaacgt ctctctggaa acaacattgt attgaatatg gtacccatgt aaacgattaa
     6301 agaatgttcg taacaaagca attagtttta atttatcttc ttctttggct aacgtttgag
     6361 gatttacttt ctgatttaag agaaccccac ctacgatttc atctgttggt aattttgaaa
     6421 cagattttaa aacagatgta gggccgtgtt gatccatatt atgtgatggt gaacaaccct
     6481 ctgctaacgg tgtacccgcg ttgcgtccat ctggagttgc taatgttcca cgtccctgcc
     6541 ctacgttggc tgatatagaa gatgttcctg aataacgaat tcctccaata ggccctcttc
     6601 cataacgtgt attaggatat ttagcaattt catcaacata aatgtcataa gcagcagtaa
     6661 ccaatttgtc agcataatca tcatcattac catacttagg tgcatcatga atcaacattt
     6721 cttgaatgac cttaccttct tctccggcat aatctgtttc cagtgcatgc caaagctgac
     6781 ttgggcttat acgttcttcc tcaaacacca attttttaat tgcagctaat gaatccgaca
     6841 aatttgcaat tccaacttgc aatcctgata tataatcata tactgctcca ccttctttaa
     6901 ggtgttttcc acgaccaata caatcatcag tcaatgctga acatagaata tcaggaactt
     6961 ctcgttccaa tgataaatca atagaatttt caacaataac actcattcgt gtcaaatatc
     7021 ttagtgtttt atcccaagca ttttctaatt cagaaaagtt cttcatatcc ttaaaatgac
     7081 caaagcttgg tgcaaaccgt ttacccgaag ccggatcaat tccatcattc atcgtgataa
     7141 gtagaacctt agggaagttc atataactca tacctgtgca acgatagccc catttccctg
     7201 gaactgccgt ttcaacacat ccaatggcac tgtaatcata agcatcatct tccaatactc
     7261 cttttgcaat aaaagaagga ataataatct catcattatt aaatgcaggc ataccaaaac
     7321 caagtttcat cacttcaata cactcattca tgaaacgagc atctaaacct gcatggtaac
     7381 gtacagttag attaggttgc ggtagatggg tttgtgcaac tgattttaat accaaataag
     7441 ataatgggtt aacagcatcc ttcttatctc gagtctgtcc accaattgta acattttgat
     7501 ataaaggact tcctgctgaa gaaaatgtat gtgattgact gcgaacctta ttaattgtaa
     7561 ttgtcttaat ccaaagattt gtcagacgtt caacaatgct atcttctgtt tctttaccac
     7621 tttctaaatc agccttcata tatggataca tatattgatc aaaacggcca tatgaaagag
     7681 agtggccatt agattcaatt tgtaaaatac attgaataaa ccaaactgat tgaatagctt
     7741 ctgcaaaagt agttgccggt tcatatggga ctctagagca aatatctgca atctcaagta
     7801 attctttctt acgtttagga tttgcatttt cggctaaact tttagcaaga gcaacaaagc
     7861 gctttgcata tactttaata gcatcgatta cgataaatat agagtcgtaa aaatgatatt
     7921 tatcaatact tgctggatct gttaaatcta gagctacctt cgctttacga gcccgctctt
     7981 caaaacctct taaaccaaat tgcaaaagtt tctgatagtt aactgctaag tgagcatctc
     8041 cagaattcat cttaccttcc ataccgaaga atcctgtttc catataaaca gacacttctt
     8101 caggtaataa ggcaccagct ctagcacgta aattattatt ttcccaaaac ggagcaatac
     8161 ttctaagttg ttcttttgtt tcttctgtaa tatagaaaac atctccatca cgcttttcaa
     8221 aaagatccaa ctcattgaga acaaattcta gcgtatattc cggaaaaata ggagcatctt
     8281 tattggaaga agcttgattt cccgcaatca tagattcttc ttcaatatag atagtcatat
     8341 tttccaaaat ttctttcagc atatatgcac gttttaggac attaggttgt tccttgtatc
     8401 gttcataggc gcgtgttgct agaactgctc tctcagcatc aatataaggt tttttattta
     8461 aaacatcttc tcgatatttg ttcatccttt cagtaaggct gccaaaatat tctgtcttga
     8521 ttgttgttct tgctacttct gtatttacca tcgtaagcac ctttctttcg aaaatatttt
     8581 tatttctttc gaaaatataa taccgatttt tatagaaatt ttcaagcttt taactagaaa
     8641 aattttaact ctctaatttt tagcacaaat gtgtgagttt aacagtaaca cacaaatacc
     8701 tctaagattg tttgatttca ttttggtaag ctaaacgatc ctgtatctta aagaatggaa
     8761 agtaaaccat tgtagacatc gccaatacta ataactgagt cacaacacct tgccatccac
     8821 ccaccaaaaa tcctgataga atagctggtg tactccaagg caatgttacc cctgagaatg
     8881 gctgcatgaa acctgttgca atagctccat atactatcac agctgcaagt acaggaacaa
     8941 gaatgaaagg tacaaacata actggattca tgacaatcgg aaatccaaat acaactggct
     9001 catttacgtt aaatattgct ggaaaagctg caacttttcc taaggcttgg tattgttttg
     9061 attttgctac aaaaagcatg gcaactacaa gaccaaacgt aatccctgaa cctgatagaa
     9121 ttaaaaatga atctaaaaac tgttgagtaa caatatgtgc tccattttct aatgatagat
     9181 taccagaggc taacatagct ttattagcat caagattaga taaaagcaga gctgtcacta
     9241 ctccatttac taccgattgc ccatgaacac caaaccacca caaaaatgat ataaagaatg
     9301 caattccaat agcaccatac aaagatccag ttaaaccttg caacggaact tgaatagcag
     9361 aataaatcat ttctatgaat gttccgccat tagtcattga cttcgctaaa atatatacaa
     9421 tcatagaaga taagaaaatt acaaatgctg gaatcattgc ttcaaactgt ttggcaatag
     9481 cttgtggaac ttgttctggc atcttaataa caatttttct ctttataaag aaggtataaa
     9541 tacttcctac taccaaacct ataatgatag caccgataat tccttggcct ccaaaccaaa
     9601 ctttactaat agcgtcccca atcgcctcac cttgtttagg gatataagat gatcttagca
     9661 aaataaagaa tgcagataca gatagaactc cagctggtaa agcctctact ccgctattct
     9721 tagcataaga ataggcaatt gaaaaacaag aaattagacc cataatagca aaagttcctg
     9781 aatatacttg cataaacggc tctgtccaat tagctccaaa aacactagca atgctcttat
     9841 ttaatccttc gaacggcaat tgtcccataa tcaagaacaa actgccaact actgtcaatg
     9901 gcaaaattgc taacatccca tcttttagag ctataatgcc acgcatattc acaaacttca
     9961 tcatcggtgc aatgattttc tgaacatcca tctttgacat aataaatctc cttttcttac
    10021 ccactaatca aagatagggc caaatctaat acttttttcc catctaacat accatagtcc
    10081 atcatcggaa taacagctat cggaacatca cacttatcac aaatttcttt tgatttatct
    10141 aatgtataag caacttgtgg acccaatagt gcaacatcta tatttggcgc ataatccgct
    10201 aatttagact gagaaaacgc ctctatttct gcctcaactc cactagattg cgctgcaatt
    10261 ttcatattat ttacaagcat accagtagaa aaacctgctg cacaaaacaa accaatcttc
    10321 accattatgt tttcctcctc ta
//
LOCUS       DAVLSL010000070        10340 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLSL010000070 DAVLSL010000000
VERSION     DAVLSL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.071"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            13..735
                     /locus_tag="VXN30_001312"
     CDS             13..735
                     /locus_tag="VXN30_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1357930.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            732..1580
                     /locus_tag="VXN30_001313"
     CDS             732..1580
                     /locus_tag="VXN30_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1357931.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1612..2541
                     /gene="psaA"
                     /locus_tag="VXN30_001314"
     CDS             1612..2541
                     /gene="psaA"
                     /locus_tag="VXN30_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1357932.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2664..3155
                     /gene="tpx"
                     /locus_tag="VXN30_001315"
     CDS             2664..3155
                     /gene="tpx"
                     /locus_tag="VXN30_001315"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1357933.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3251..5959)
                     /locus_tag="VXN30_001316"
     CDS             complement(3251..5959)
                     /locus_tag="VXN30_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1357934.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGRAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5961..6662)
                     /locus_tag="VXN30_001317"
     CDS             complement(5961..6662)
                     /locus_tag="VXN30_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1357935.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6912..9323)
                     /locus_tag="VXN30_001318"
     CDS             complement(6912..9323)
                     /locus_tag="VXN30_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1357936.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9376..10068)
                     /locus_tag="VXN30_001319"
     CDS             complement(9376..10068)
                     /locus_tag="VXN30_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1357937.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10119..>10340)
                     /locus_tag="VXN30_001320"
     CDS             complement(10119..>10340)
                     /locus_tag="VXN30_001320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357938.1"
                     /translation="TKAEEGFKGTAMENNDSFTKLKESTQKLFDAQKKRLNNEDRIET
                     TKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
BASE COUNT         2987 a         2392 c         1959 g         3002 t
ORIGIN      
        1 gaatacaagt ttatgatacg tatcgaaaac ctcagtgtct cctacaaaga aacgttggca
       61 cttaaggata tttcactagt gctccatgga ccaacaatta ccggcatcat tggtccaaac
      121 ggcgctggga aatcaacact attaaaaggt atgctgggaa ttatcccaca tcaaggtcag
      181 gcatttctcg atgacaagga agttaaaaaa tccttacacc gaattgccta tgtcgaacaa
      241 aaaatcaata tcgactacaa ctttcccatc aaggtcaagg aatgcgtctc gttaggacta
      301 tttccctcta ttcctctctt tcgaagttta aaggctaaac attggaagaa agtgcaagag
      361 gcccttgaaa tcgtcggcct agctgactac gctgaacgtc aaattagtca actgtctgga
      421 ggtcaattcc agcgggtctt gattgccaga tgtttggtgc aggaagccga ctatatcctc
      481 ttggatgaac cctttgctgg gattgactct gtcagtgagg aaatcatcat gaatacgctg
      541 agagatttga aaaaagctgg gaagacggtt ctcatcgttc accacgacct cagcaagatt
      601 ccccactact tcgatcaagt cttacttgtc aatcgagaag tgattgcctt tggtccaaca
      661 aaagaaactt ttaccgaaac caatctaaaa gaagcttacg gtaatcaact ctttttcaat
      721 ggaggtgacc tatgattgca gaatttatcg atggattgca aaaattccat ttcctacaaa
      781 atgccttgat aacagctatt gtcgtcggga ttgtagctgg agccgtggga tgtttcatta
      841 ttctacgcgg gatgtcactc atgggagatg ccatttcaca tgctgtctta ccaggtgtag
      901 ccctctcctt catcttgggc cttgacttct ttatcggagc cattgtcttt ggattgctag
      961 ctgccatcat cattacctac atcaaaggaa actcgattat caaaagcgat accgccatcg
     1021 gcattacctt ttcttctttc ttagccctcg gtatcatctt gattggtgtc gctaaaagtt
     1081 caactgacct tttccatatc ctttttggta atattctggc cgtccaagat acggatatgt
     1141 ttattactat gggtgtgggg gcagccatcc tcttgttaat ctggattttc ttcaagcaac
     1201 tcttgataac ttcctttgat gaactcttgg ctaaagccat gggaatgcct gtcaatttct
     1261 atcactacct tctcatggta ctcctgactc tcgtgtctgt gacagccatg caaagtgtcg
     1321 gaactatcct gattgtagcc atgctgatta ccccagctgc aactgcttat ctgtatgcta
     1381 atagtctgaa aagcatgatt ttcctttcct caaccttcgg agctactgct tcagttttgg
     1441 gactctttat tggctatagt tttaatgttg cggcaggttc tagtatcgtg cttacagctg
     1501 ctagtttctt tctcattagc ttctttatcg ctcccaaaca acgatatttg aaactgaaaa
     1561 ataaacattt gttaaaataa ggggcaaagc cctaataaat tggaggatct aatgaaaaaa
     1621 ttaggtacat tactcgttct ctttctttct gcaatcattc ttgtagcatg tgctagcgga
     1681 aaaaaagata caacttctgg tcaaaaacta aaagttgttg ctacaaactc aatcatcgct
     1741 gatattacta aaaatattgc tggtgacaaa attgaccttc atagtatcgt tccgattggg
     1801 caagacccac acgaatacga accacttcct gaagacgtta agaaaacttc tgaggctgat
     1861 ttgattttct ataacggtat caaccttgaa acaggtggca atgcttggtt tacaaaattg
     1921 gtagaaaatg ccaagaaaac tgaaaacaaa gactacttcg cagtcagcga cggcgttgat
     1981 gttatctacc ttgaaggtca aaatgaaaaa ggaaaagaag acccacacgc ttggcttaac
     2041 cttgaaaacg gtattatttt tgctaaaaat atcgccaaac aattgagcgc caaagaccct
     2101 aacaataaag aattctatga aaaaaatctc aaagaatata ctgataagtt agacaaactt
     2161 gataaagaaa gtaaggataa atttaataag atccctgctg aaaagaaact cattgtaacc
     2221 agcgaaggag cattcaaata cttctctaaa gcctatggtg tcccaagtgc ctacatctgg
     2281 gaaatcaata ctgaagaaga aggaactcct gaacaaatca agaccttggt tgaaaaactt
     2341 cgccaaacaa aagttccatc actctttgta gaatcaagtg tggatgaccg tccaatgaaa
     2401 actgtttctc aagacacaaa catcccaatc tacgcacaaa tctttactga ctctatcgca
     2461 gaacaaggta aagaaggcga cagctactac agcatgatga aatacaacct tgacaagatt
     2521 gctgaaggat tggcaaaata agcctctgaa aaacgtcatt ctcatgtgag ctggcgtttt
     2581 ttctatgcac acatttccgg tcaaatcatt ggaaaattct gactgtttca gatacaatgg
     2641 aagaaaaaag attggagtat cctatggtaa cttttctcgg aaatcctgtg agctttacag
     2701 gtaaacaact acaagtcggc gacaaggcgc ttgatttttc tcttactaca acagatcttt
     2761 ctaaaaaatc tctggctgat tttgatggca agaaaaaagt cttgagtgtc gttccttcta
     2821 tcgatacagg catctgctca actcaaacac gtcgttttaa tgaagaattg gctggactgg
     2881 acaacacggt cgtattgact gtttcaatgg acctaccttt tgctcaaaaa cgttggtgcg
     2941 gtgctgaagg ccttgacaat gccattatgc tttcagacta ctttgaccat tctttcggac
     3001 gcgattatgc cctcttgatc aacgaatggc acctattagc acgcgcagtc tttgtcctcg
     3061 atactgacaa tacgattcgc tacgttgaat acgtggataa tatcaattct gagccaaact
     3121 tcgaagccgc aattgcagct gctaaagccc tatagaaaaa atcctctgtc acaaagagtt
     3181 ccctgctctt tgaaacggag gatttttgaa tattagtaaa tagaagttca atcagctaaa
     3241 aataactacc ttactctaca gatttcaggg cttctaacat gtcaaccttt ctcagataat
     3301 aattgacgaa gaaaccaagc aaggtcaaaa tgatgcttac tgctgccact gggattacat
     3361 agacttccca gcctacctgc ggataaaaga gaatagtcgc aggcgaaatc atttgaatca
     3421 aaaattggtg taaatagaaa ccagctacca gaccaagtac gattcccaca agggacagca
     3481 caatcgtctc acggtaaatg tagagggtga cttcattatt atgaaaaccg agaaccttga
     3541 tagtggagag ttcacggatt ctctcagcta cgttgatatt ggtcagattg taaaggatga
     3601 caatagctaa tagaaccgat acgatgacca agatggtcat ggtctgattg agtgagctag
     3661 cgatagagtc gaagagtcga atggctgaag cattttggac aacactggac accgcagatt
     3721 gattcataag caagcccgcc tgactttcga tactagttgc actggtatcc cttaatgaga
     3781 ccagataagt gttggcttgg ggtagctgtc cgtaaagttg ctcatagcta gcctgactca
     3841 tataaataaa gtgaccaacg tagttctcag taatagcagc gacctttagt tccttacctt
     3901 caatttctaa agtctgccca accttgacac ctgccagctg ggcgagttta gctgtaataa
     3961 cgatgccatc ttttaatgtc agctcctgct gatgatgttg aagatggata aagggagtca
     4021 aatcttcctt ctctatcatc ataagagtaa tgttttgaag accagccctg cccttgaaat
     4081 ccttgtctag cgttttagaa tagattttct ggtaggctag tatctcctgc cctttcaaca
     4141 cttctgctag ctctaccttg tcctgattgg tcgcactagg attttcagag acaagcatct
     4201 gatactgttg gatttgttga aactgtttag acggaactcc tgctacagaa gattggattc
     4261 ccaaacctgc aaagagcaga gctacagaac ctgccacacc aaagattgtc atcaacattc
     4321 tctgcttata acgaaagatg ttgcgggctg ttaccttatg agtaaaactg agacgacgcc
     4381 agataaaacc gatacgctcc aataagattt tagctccttt gacaggaggt ttaggaagta
     4441 gaagctgggc tgcttcgtca tgaagttccc tccaagccac cagataggct ggtaacacac
     4501 tcgccaacca gctcaagaca agagctagta agctataggt ccaatagaac tgaatctggg
     4561 tttctcccac caccatgcct tttgtaatga cacttgaaat tacactggct agcaaataat
     4621 gaccaagtat actacctaga gccgttccga cagtcccagc tactagtcca taaaggagaa
     4681 acttggcgat aatatcctta ctacgataac ccaaggcctt aaaaatccct gcatgagttc
     4741 gctcttcgtc tacaaagcga gtcatggtcg taaaggtcac catggctgct acggcataaa
     4801 gtaccacagg aaagatattg cccactgctc gaatactgga tgaagcattg ctatacataa
     4861 gatagccctg accacctggc atggtctgac gattataaac ctgatacctt ggctccgcca
     4921 aatcatccaa gacttgctga tgtttttcta atttttcctt ttcttgggct agattttgtt
     4981 cagcttgctt tagtttgtcc tcttccttgc ccagttcctg cttggcttgg gtaagttgag
     5041 cactagcctg ctctctctga gcttgaggaa acaaggctag ttgactttct tgagcctgta
     5101 tacgagcttg agcagctgct aaacgacgct tgccttcctg caaattagtc tgagccttgt
     5161 caagagtctc ttgccccttg tctagagact cttgtccttc ttttttcaaa agttgcagac
     5221 gtaccttgcc attatctgat aaagtttgtt caaggtcttc ctgatgttgc ttggattttt
     5281 cttcataagc tgatgaaaag gcatttaaac ctgctaaatc ttgatatttc aaacgagcta
     5341 tattgtagac tttctgatca aattgactag gtaaaatcac cccataggct gtcagagtcc
     5401 cacttccact tcctgcgtag cccatatctc gctgggagag gatttcagcc gaatccacaa
     5461 aaccagtaat ggtataagta tggtctttta aagaggaatg accctcttct ttttctttaa
     5521 aactaatctc ctgtcccacg ctgtattggc cttgcaaatg agtggccaaa gcgatttccc
     5581 tgtctgactg aggaagtcgt ccctttctta gctgaaaggt tgaaattcgc tctggtttgg
     5641 agtacagtcg aatggcatcc tgcccattat ccatagtcac atctgtcaaa tagccaaact
     5701 cgacctctgc gccctccgtc tgttttagtt cttcttggtc tgcttgatcc aagccatagt
     5761 tagacatgac tgccaaatcc aaggtttgag cagttgttaa ataagcatta gctgtcgcct
     5821 ccatgttggg actggttact ttgaggccta ctaaggctag agatcccaac atcatcagga
     5881 tcaagatgga taaaaaacgc cccttggagt ctgtgaagga ctgaattaag tccttccaat
     5941 aagtttttcg cttgatcatg ctagtactcc aaactgtcaa tatcctgagg atgctggttg
     6001 agcaccacat ccttgacact ggcatcgtgc atatgaatca cgcgatcagc aatgggcgcc
     6061 aaagctccat tatgagtcac gatgatcacc gtcgctccct tttgacgaga catgtcttgg
     6121 agaattttca aaacctgctt gcccgtctga taatccaagg ctccagtcgg ttcatcacaa
     6181 aggagaattt taggattttt ggctaccgcg cgtgcaatgg agactcgctg ttgctcccct
     6241 ccagaaagct gggctggaaa gttattgaga cgatgagcca gacctacatc tgtcaagacc
     6301 tgatcaggat tcaaggcatc tgtcacaatt tcagaagcca gttccacatt ttccttagct
     6361 gtcagattag aaactagatt ataaaactga aaaacaaacc ccacatcatt tctacggtaa
     6421 ttggtgcgct ggtgggaact ataatccgca atattaacac catcaatcca gatttcccct
     6481 tcatcattgg tatccattcc cccaagaagg ttaagaactg ttgacttgcc tgcacctgaa
     6541 gcaccaagga taataaccag ctcccccttt tcaatctcaa aattcacatc acgattggcc
     6601 acaatctccg tgtccccaac ctgataacgc ttgtaacagt gtttcatctc aatataagcc
     6661 atcagaccca ccctctatca aacaaattat actcctcgaa aatctctctt caaaccacgt
     6721 cagcttccat ctacaacctc aaaacactgt tttgagcaac ctgcggctag cttcctagtt
     6781 tgtgctcttt gattttcatt gagtattact tcctattatc attataacgc tttttcaact
     6841 agttggaaac tgcctatatt cttaaatcct tattaaaagg aagtttcaaa ttactgcctt
     6901 ataacatctt cttaaaaata aaattgaaca agatcacctt ctgctgttgc caccgaaata
     6961 caatctttcc ccatgcattt cgcttttatt ttttcttgat tcattttaat cacactcttc
     7021 tcaatatctg gataagaaac tcgcaaatct cctccactcc ttgataaaat ggtcaactgt
     7081 aagagttttt tatcttccca gctcatgctc acttcaaaat gtccacgtgc cattaagcct
     7141 gaaacagaac ctgttgacca agcatcaggt agggcagcta gaggtaccag ataagctgca
     7201 tgagactgga gtaacatttc tgccatgcca ctagtagcac caaaattacc atctatctga
     7261 aaaggaggat ggctacacca aagatttggc aaggtggatg tttttaactg ctctgccaat
     7321 aatttatggg ctcgattgcc atctcccaaa cgcgcccaga gattgatctt attagccttg
     7381 gaccagcctg tgccgccatc tccacgatca ttgaggctag cacgtgccgc ttcaatatac
     7441 tcttgtccct tgtagctaaa gagattgcca ggatagagtc ccactagatg ggaagcgtgc
     7501 cgatgctggg cctccacttt ctcattttga aaatactgct cttcctcctc ataccactcc
     7561 ctgattcgac cagattgagt gatttgcaaa ggatttagta aatcagactt ctccttaacc
     7621 tcagtcaaca agtcctcatc cagtcccaat tcctgagcag cctgaataaa atcatgaaat
     7681 aactgccaaa tcagagattg gtcataagta ttgccaatcg aaatcggccc atgttctggg
     7741 gaataagacg gagaagacac ccaacgctgc gcctgctgat ccttatgtaa aaaggcattc
     7801 caaaaacgaa ccgtttccct caacatggga taaattttct ccctgagata gtcttggtcc
     7861 ctataaaatg aataggcttc ataaacagtt tgcatcatcc acgcattggc agctggtgac
     7921 caaccccaat agtaatccca accaggtgcc gtccaaccaa agggagtcgc ttgagtatga
     7981 accaaccaac cattttcctc acctttttga gagacgattc ctgcatactt tacagccgct
     8041 agacgaccat agacacgcaa atcatctaca tagttgatga ccggaaagac cgcctctagg
     8101 agattggtaa cataggctgg ccaataattc atctgcagat tgacatttaa gtgatagtcc
     8161 gaattccaag gaggattgtc gaccgcattc cagactccct gtaggttagc tggtagagca
     8221 tctgggcagt ctctggacga actaatcaat aaataccgtc catactggaa gaacagctcc
     8281 tccaaagcct gcccttcttg tggcttataa ttttttaaca aatcatctgt agtgaatgcg
     8341 tcaacattag cttccaaatc caattgaaca cgctggaata aggcttggta gtcctcgata
     8401 tgccttgatt tcaattgggt atagcccttt tctttagctg tgtccaccaa gtctatcacc
     8461 tgttgctcta aatctagttt cttgcgataa ttgctagcag gattttgggc aaaatccgtc
     8521 ttagcggcca agaagagatt ggcataactg gctcctgata tctgaaccct atctgaccaa
     8581 actctaatat ctccatccgt ttcccaagct agataactag caaaccgcag atcattatcc
     8641 ttaactcttc ccttcatcaa gatatgagaa tcagtaatat ccaacttaca ctccttgtaa
     8701 tcagattttt cctgctcata ctttccatca gaagccaaat cacaggtcaa ggatagttct
     8761 atagtaaaat ctagagtttc cgccccttcc ttagtaaaac gctgaaccaa gagatcatct
     8821 ggaaaactcg caaaagcttc acgttcaaat cgcgttccct tatagacata agaagtcgtc
     8881 acaagtgcct tactaatatt cagctgtctc tgatagtccg tcacctgaga caaagtcgta
     8941 ccttgctggc tgaactcaat gtgaatatcc ccaaaagaca gataggtccc atattgactc
     9001 gtttttggcc caattaggtg ctgctcagcc agttccttag ccagattgta atctctcttc
     9061 tccaaagcct gccgaatctc agctaaaaaa acatactgat cctgaagatt tccaccctga
     9121 taatctgaac tatcaggaag tggacctcca gaccagagac ttttttcatt aaattgaatc
     9181 cgttcagccc ctataaggcc aaatactttt gctcctaaag aaccattgcc tatcggtaaa
     9241 gcttcttctt cccaacccat gtaagtggtt gaaactggtt gcttgtaggc cagtacataa
     9301 tcttgttttt tattccttat catacttcca tactaacata aaaagcctag aaatcaattc
     9361 tagacttaca attttttatt ttccaaggta gtattcagaa acgacgttca atttttcgtc
     9421 gaattcgaat accaatggtg ggaagttagg gatttccacg tccatgatct cgtcatctga
     9481 caaacctttg atgtgtttta caagggcacg gattgagtta ccgtgagctc ctacgaatac
     9541 gtttttacca tctttaagag ctggagcgat tttatcttcc cagaatggaa gagcacgttc
     9601 caaagtcact ttcaagtttt cagcatctgg gattactgag tcgtcaagtg aagcataacg
     9661 acggtctgtg tgagctgagt gctcatcatc acggtccata tttggaggca atacatcgta
     9721 tgaacgacgc cagatgtgaa cttgctcatc accaaattgt tcagcagctt cagctttatt
     9781 tttaccagtc aaaccaccgt agtgacgttc gttcaagcgc catgattttt caactggaac
     9841 ccacaattgg tcagaagctt caagagccaa gttagttgtt ttgatagcac gtttcaatac
     9901 tgaagtgtaa gcttggtcaa atttgatacc agcttctttg atcaatttac cagcgtcaat
     9961 cgcttgttgt gtaccttttt cagacaaatc aacatcagcc caaccagtga aaaggttagc
    10021 tttgttccat tcagactcac cgtggcgagc aaaaaccaat tttaccatta gatggattct
    10081 cctttaaatt ttccgaggtt tcccctcgtt tatctattct attttacaca atttttcaca
    10141 aaaaagctag tcaaaaccaa aaaggaaagg actgtttgac aatgtttggc aatcacattg
    10201 tttttagttg tttctattct atcttcatta tttaaccttt ttttctgcgc atcgaataat
    10261 ttttgagttg actcttttag tttagtaaac gagtcattat tttccatagc tgtgcctttg
    10321 aaaccttcct cagctttagt
//
LOCUS       DAVLSL010000071         9937 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLSL010000071 DAVLSL010000000
VERSION     DAVLSL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.072"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(63..1004)
                     /locus_tag="VXN30_001321"
     CDS             complement(63..1004)
                     /locus_tag="VXN30_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1357939.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(982..2745)
                     /gene="aspS"
                     /locus_tag="VXN30_001322"
     CDS             complement(982..2745)
                     /gene="aspS"
                     /locus_tag="VXN30_001322"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1357940.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(3026..3106)
                     /locus_tag="VXN30_001323"
                     /pseudo
     CDS             complement(3026..3106)
                     /locus_tag="VXN30_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(3142..3372)
                     /locus_tag="VXN30_001324"
     CDS             complement(3142..3372)
                     /locus_tag="VXN30_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357941.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(3390..4031)
                     /locus_tag="VXN30_001325"
     CDS             complement(3390..4031)
                     /locus_tag="VXN30_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1357942.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(4043..4777)
                     /locus_tag="VXN30_001326"
     CDS             complement(4043..4777)
                     /locus_tag="VXN30_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1357943.1"
                     /translation="MKKKRRLLFLMSIVFGGVLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(4780..4977)
                     /locus_tag="VXN30_001327"
     CDS             complement(4780..4977)
                     /locus_tag="VXN30_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357944.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(4977..5183)
                     /locus_tag="VXN30_001328"
     CDS             complement(4977..5183)
                     /locus_tag="VXN30_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1357945.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(5193..5540)
                     /locus_tag="VXN30_001329"
     CDS             complement(5193..5540)
                     /locus_tag="VXN30_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357946.1"
                     /translation="MIDKVVRNLLLTFFLCKMTKIINFLTTILVKKKKMCYNVSKLRE
                     KKKGNAEMSRLLQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQ
                     RIEDIQTVEFDGE"
     gene            complement(5612..5692)
                     /locus_tag="VXN30_001330"
                     /pseudo
     CDS             complement(5612..5692)
                     /locus_tag="VXN30_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5683..6972)
                     /locus_tag="VXN30_001331"
     CDS             complement(5683..6972)
                     /locus_tag="VXN30_001331"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1357947.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRAYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(7050..8297)
                     /locus_tag="VXN30_001332"
     CDS             complement(7050..8297)
                     /locus_tag="VXN30_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1357948.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMLLPTLFTVFAGIRADYTRAKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINFLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(8488..9339)
                     /gene="rgg"
                     /locus_tag="VXN30_001333"
     CDS             complement(8488..9339)
                     /gene="rgg"
                     /locus_tag="VXN30_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1357949.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNVAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFYTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            9463..9804
                     /locus_tag="VXN30_001334"
     CDS             9463..9804
                     /locus_tag="VXN30_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1357950.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         3117 a         2166 c         1873 g         2781 t
ORIGIN      
        1 aacatatata cggtaaggtg aagctgacgt ggtttgaaga gattttcgaa aagtattaat
       61 cactaattat ctatctcaac aaatcttcct agaatatgaa cattttccga gacagagaca
      121 aaggagcttg gatccacttg tgtcataatc tgtttaaatt cattaaactc tgcacgtgta
      181 atgacagtga ttaaaactgc ctttctctcg tgattatagg ttccttctgc atcgtggatc
      241 atggttgctc cgcggtgcaa ttttttatgg attttttcaa ttaccttctc tggatgattt
      301 gtcacaatca tggcctgcat acgcttttgc ttagtaaaga ctgcgtctgt cacacggcta
      361 gagacaaaga tggtaatcat agaataaaga gcgtatttcc aaccaaaggt caaacctgct
      421 atcagcatga tagttccatt taccaagaaa gaaatactac cgacattctt acccgttttc
      481 ttgcgaatag tcaggctgac gatatccgtc ccaccactgg agatattgtt tcgaagagca
      541 aaaccaatcc ccaaacccat aacaacaccc ccaaaaaggg aattgataat gggatcctcc
      601 gtcaaggttg ccacagggac aaactggata aagaaggaac tcatagatac cgtgataaag
      661 gtaaagacgg tgaacttatg gccaatctga taccaagcta agaccatcaa agggaagtta
      721 atggcgtaga agcttagcga aatcggaata tgaaaaccaa accagtgatt actcaaggca
      781 gagataatct gtgccagacc tgttgcacca ctcgaataca catgccctgg ttggaaaaag
      841 aaattaactg ctactgctga taaaaaacca tagaccagag aggccgaaat cttctcatca
      901 tacttttctc gagagatact ttgtaagaca cgtaaaattt ttatctgata agcaaagcgg
      961 cgcagataat agcgccaccg cttaattcgt tttgtttgtt tcatcttctt ctacttgtaa
     1021 gctgagttcc tctagttgtt tgagagcgac tgttgatgga gcttgtgtca ttgggtcagt
     1081 tgccttgttg ttcttaggaa aggcaatgac ttcacggata ttttcttctc cagcaagcaa
     1141 catgacaaaa cggtcaagcc cgatagccaa accaccgtgt ggtgggaaac catagtccat
     1201 ggcttcaaga aggaaaccaa actggtcatt ggcttcttca gttgagaaac caagagcctt
     1261 gaacatgcgt tcttgaaggt ctttttggtt gatacgaagg ctaccaccac caagctcata
     1321 accgttcaag acgatatcgt aagcaatggc acgaacctta gccaaatcac cttctaattc
     1381 gtgagcagtc tcttcctgtg gaagggtgaa aggatggtgg gcgctcatgt agcggccttc
     1441 ttcttcagac cattcaaaca tcggccagtc aaccacccaa aggaagttga acttatcatt
     1501 atcaatcaag ccaagctctt tagcaatacg tccacgaagg gcacccagtg ttgcattagc
     1561 cacttcaagc gtatccgcca caaagagaac caagtcctta tcttcaagaa caagcgctgt
     1621 tgtcaattct tcttggatac cagtcaagaa cttggcaact ggtccgttta attctccatc
     1681 aaccaccttg acccaagcaa gacctttggc accatactgt ttggctactt ccgtcatctt
     1741 gtcgatgtct ttacgtgaat agttgtccgc agctcctgtg accacaatcg cttttacagc
     1801 aggtgcttct gaaaagactt taaagtctac acctttgacc acttctgtca agtcctgaag
     1861 caacatgtca aaacgagtat ctggcttgtc agaaccgtaa agagccatag catcatcgta
     1921 tttcatacga gggaacggta gcgttacttc gatgcctttt gtttccttca tcacgcgcgc
     1981 gatcaagcct tctgtaatat cttggatttc ttgctcagta aggaaggacg tttccaagtc
     2041 gacctgagta aattcaggct ggcggtctcc acgcaagtcc tcgtcacgga aacatttaac
     2101 gatttggtag taacggtcaa aaccagcatt catcaagagc tgtttcgtga tttgtggact
     2161 ttgaggaaga gcgtaaaaat gccccttatt aacacgagac ggcactaaat aatcacgcgc
     2221 cccttcaggc gttgacttag aaaggaatgg tgtctccacg tcgataaact ccaactcatc
     2281 caagtagttg cggatagagt gggtcacctt ggcacgaagt ttaagatttt ccaacatttc
     2341 tggacgacga aggtcaaggt aacggtaacg caaacgtgta tcgtcatttg cctcaatgcc
     2401 atccttaatc tcaaatggtg ttgtcttagc tgtgttaagc acaataagag ctgtcacgtt
     2461 taactcaact gcaccagttg gcaacttatc attggcttgc tcacgcgcag cgacctgacc
     2521 agtcacctca ataacaaatt cgctacgaag gctttcagct gttgccataa cctctgcaga
     2581 tactttttca gggttgataa ccaactgcat gattccttca cggtcacgaa gatcgataaa
     2641 gatcaaacca ccaaggtcac gacgacggcc aacccatcct ttcaaggtta tttcttgtcc
     2701 gatgtgttcc tcacgaacac gaccagcata catactacgt ttcattattt ctctcctctt
     2761 ttattctgtt actattttac cataaaagcg caggctcttc atgaaaatca tcagaaaagt
     2821 ttgccagtct ttaaaagtca ggtgaaagcc ctaaaaatta gcgctaatac tcttcgaaaa
     2881 tctcttcaaa ccacgtcagc gtcgccttac cgtaggtatt gttactgact tcgtcagttt
     2941 catctacaac ctcaaaacca tgttttgagc tgacttcgtc agtttcatct acaacctcaa
     3001 aaccatgttt tgagctgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc
     3061 aacctgcggc tagcttccta gtttgcactt tgattttcat tgagtataat acaaaaatcc
     3121 gatgaacttc accggactct tttattttga atttttgcct gctttacgct tttcagcgat
     3181 ttcggctgcc tttcgaggca agacaatttc cgttatgtaa gccgtcccaa aacgcagtac
     3241 acctgcaata ggagcaaaga caactgctag atagttatag aagaaatcgc ctttgaaggc
     3301 ataagctagc gctccaatga tgaaaaatag aacgactgcc tgaatcactg ctaataaaat
     3361 tactcgtttc atgtgacctc ctgactctat tatagcatga gaatcatcaa aaagccgact
     3421 aaattattca aagcgtgaag agaaatactg tagaccagac cttttctgct aatgtaagcc
     3481 aaacccaaac taaaaccaag gctaaaatag acaaaaaatt gttgcacatc acctggaaaa
     3541 tgaatcaagg caaatagaag actagatacc agaagaaaaa tcagggttcg tttactattg
     3601 tcctgcttag gaaagagata gcgtgctaac atccctctaa aaacaatctc ttccgtcaaa
     3661 ggagcaaaaa taaccacagc aaagaatgag aaaagtggtt gagacaaggt caagtctgtc
     3721 gctatttgtt gatttactga aggatcatct ggcaagaaga attgaacgac cagagataag
     3781 aaccaaacca aggcaggaag ccaaataaat cggttaaagc cgctcttctc aatataaaca
     3841 ggagctttct gataccattt gtaaatgccg tacacatata ctccagccaa gaccacatag
     3901 agtagagtaa cagcataggg tgaagcgcct aaagcaagcg acgcagtcgc gagcccctga
     3961 ataaagccat agataaagaa aaaggataga agggctagaa gaaaccagcc aaggttttta
     4021 agtaatttca tagataactc ctttatttga aataacgttt taccataggt aactgcatca
     4081 cattgatata aacatggatg gctcctacaa gcaagaaagc tagtaactga atctctcctg
     4141 tcaagaaaga aaggataata agaaaaatat ataaggctgg taagacatat tggtgtaatt
     4201 ggaataaaat tcgaaaactc tgttccaaat tagcctgacg ctccccttca tcataagaat
     4261 ttatatagtt caagacatcc tttggtgtag cgaaaaattc caaatcaaac tgacgaacaa
     4321 tcgcaatggt tttaaaaaga gatttttgag cgactaagaa taccacaaag agtaagaaag
     4381 aaaggaaaaa tgtttgaggg tttgtatgca atataatcac ctcacttaat gaaataaaaa
     4441 tagccaatgg aatcgctaca cctgtaatat taaaagcaat ggttccaaac tcaagattcc
     4501 gatacatttg cacataatag gtttcattca gatcgtcatc catttcctct tgatacaagg
     4561 aatgaaattt tctgcttttc tttaagaaat tgaaagtcaa aaacatacta atgaaaccta
     4621 tcagtaaaca aatagctgat atccatggca tcaaggcttt tacatctaaa ataatttcgt
     4681 gggattcgac acgtgcctta aacatcccta caaacatgcc caagaccccc ccaaagacaa
     4741 tagacatcaa aaataacaat ctacgtttct ttttcatatt cattctcctt tttcacttgc
     4801 tagatttttg gatttctttt caatccattc aattactggg atgagagcaa agtagaccca
     4861 aacaaattgg tcgctttgat agggattaaa ccagcttagg tccatcccaa tcagtagaaa
     4921 tacgctgact aataaagcta tgaccactac ataataaatc actttatact tgttcatcac
     4981 tcgtcctcct ccaaacgaaa taccgattcg actgtttcgt tgaaaatttg agatattttc
     5041 agggcaatga taatggatgg ggtgtactca tcccgttcta gtaggctaat ggtctgtctg
     5101 gaaacccctg ccagtttggc taggtcggtt tgattgagac catcgcgagc tcgaagctct
     5161 tttagacgat tttttagttg catgttacac acctactctc cgtcaaattc aacggtttgg
     5221 atatcttcaa tacgttgcaa cttgaatttt tctttcccct ttttatcaac actacgtagt
     5281 ttgacccatt cctcgtcaac atccacaact tcccagttat ctggcccaat atacactccc
     5341 gttataattg gttcctttcc aatcatttct tgtaataatc tcgacatttc tgcgtttcct
     5401 ttcttctttt ctcttaactt gcttacattg taacacatct ttttcttttt gacaagtata
     5461 gttgtcaaaa agtttatgat ttttgtcatt ttgcaaagga aaaaggtcag gagtaagttc
     5521 ctgaccactt tatctatcat taatactctt ctaaaatctc ttcaaaccac gtcagcttca
     5581 ccttgccgta ggtatggtta ctgacttcgt cagtttcatc tacaacctca aaacagtgtt
     5641 ttgagcaacc tgcggctagc ttcctagttt gctctttgat tttcattgag tataaaatcc
     5701 taatttttca aagatttctg agaagttttg gctgattgtc tcaagtgaca cttgcacttc
     5761 ttctcgggtt tggttgttct tgactgtcac ttgtctgctt tcgacttcgc tctctcctag
     5821 ggtgatgagg gtcttagccg caaagacatc ggctgacttg aactgagctt tgagcttacg
     5881 gttgaggtaa tcacgctctg ctttgaaacc ttgttggcga agagcttgga ctagttctag
     5941 ggctttgaca tttgctccat cacccaagac tgcgatatag acatctaggg cattttcgat
     6001 agggagggcc acgccttgtt tttcaaggat gagaagcagg cgctctacac caagcccaaa
     6061 accaaatcca gcagtttcag ggcctccaaa gtaagcaacc aaaccatcgt agcgaccacc
     6121 cgcacagact gtcaagtcat tgccctcaat ctctgtgata aactcgaaaa tggtgtggtt
     6181 gtagtagtcc agaccacgca ccatattggt atcgatgatg taatctactc caagattttc
     6241 caacatctga cgcacagcat caaaatgagc ttggctttct tcatcaagaa agtccaagat
     6301 agacggcgca ttctctactg ccaccttgtc ttctttttcc ttagagtcca agacacgaag
     6361 aggattttcc tccaagcgac gttggctatc cttagacaag gtctccttga gcggtgtcaa
     6421 atagtcaatc aaggcttggc ggtaggctgc acggctctca ggatttccaa gagtgttgag
     6481 gtgcaatttg acaccttgaa taccgatttc cttcaaaaaa tgggctgcca taacgattgt
     6541 ttccacatcg gtagctggat tgctagagcc aaaacactca acaccaatct ggtggaattg
     6601 gcgcaagcgc cctgcctgtg gacgctcata acggaacata ggtcccatat agtagaactt
     6661 gcttggcttt tgcacttctg gggcgaagag tttattttcc acataggcac ggacaacggg
     6721 cgcagttcct tctggacgga gggtaatatg gcggtcaccc ttgtcataaa aatcgtacat
     6781 ttccttggtt acgatatccg ttgtatctcc gacagagcga ctgataacct cgtaatgctc
     6841 aaaaataggc gtgcgcactt ctgcatagtt atagcgcttg aaaatctcac gggcaaagcc
     6901 ctcaacgtac tgccacttgg cagattcagc aggtaaaata tcctgcgttc cttttggttt
     6961 ttgtaatttc atagggaatc ctctttaaac ttaatagtct tattttacca taaataaagg
     7021 gattaaaaca gtaagaaaaa aattaggatt tagatatcat ttttgagatt aagaattgtc
     7081 aaaaaaatag ctagcaagga aaaaccaaca aatagcatcc aagtcaactg tatattccat
     7141 acggctacta gtgaaaaaca agctgttccc acaggtatgg ataaggtaaa caatagacct
     7201 aaaaaattac tagtacgagc tagaacctct ggagctagat ttttcatgag catggcacta
     7261 atctttggtt gaactttacc agacacatac agggtaaaga agagaaatag caaaccaagc
     7321 acgacttgat tgaataaatt agccaaacca actagactaa gtcctacggt cgcccacatc
     7381 atcaatctag gcaaggactg cttcccaaaa taatcattgc ccgtaaggct actgatgatg
     7441 attgctacta aaacacagaa ttgattgata aatagtgcct ctgtataaga aaaattcaag
     7501 agagaatggc tcaaaaagaa gatattataa attccaccca aagcgccacc caagaaatta
     7561 ataagcaaga cagcaaagag cataaaacca aagtttttct gtccactttt aagaaaaacg
     7621 agacgtaaat ttcggtaaat tgttaggaac tggtctttga tagaaagctt ctcatttttt
     7681 aagttttcac catcagcaga tgacattgac aggctcaatt tgctttttcc taaaaagagg
     7741 atagtggctg atactaggaa aaagcaggca ttgattcccg caacgagaga aaaattgttg
     7801 accgatagag ctaagagcca gactccgaaa gcttgaccac caatagctga aatataggtg
     7861 atgaactgtg aaaaagaata agcctccatc aaatcatctt cagctacttt ttccttaata
     7921 agaggcatac gcaggccacc tgcaaaatca ctgatgatat cactaatgac attgatcaaa
     7981 cacaggctag aaaaggcaaa taaactagct tgctgaacaa ctagggctgc cagaaaaaat
     8041 aaaaccgcct gaaacaaacc gctatagacc atccatttga ccttggccct cgtgtaatct
     8101 gcccgaatcc ctgcaaaaac tgtaaagaga gtcggaagaa gcatgacaat attcgccata
     8161 gcaacagcaa aagatgcttg tgacaaggtc gatgcataga cgataaagac cagattgaaa
     8221 atcgaagcac caaaagcatt gaagaagcgt gataaagtca aaattctata agctggattt
     8281 ctaaacaata gtttcataga taatctccct ccttgttcta agttctacat caagaatagc
     8341 tttagtatag cacttatgaa tttgcgaaac gattcttttg aatatatgca acaaacaaaa
     8401 tgagcaaaaa aacgatagaa cttttgtaat gacctgagag agggcaacag ttctatcgct
     8461 tcaaattttt tcttggtttg cagatattca acattcggtc gtatttctat agtattcggc
     8521 agatttatta cagccaagca tctcaaaaat acggacagca tcctccatct ttttctgacc
     8581 ttccttgact ctaccttgct tgctatcaag gagaccttct gcccacagat aaacaattcg
     8641 gaaataggtc tcattttcct tgtagaaatg ctcttcgata acacgtttaa aataataggc
     8701 attggtaaat tcttcacact caatactagc taaaaagcca ttcaatagta tagtataaaa
     8761 aaggtttcga ttgccagaca tttccattag aaaatcggat ttacgtacca tttctcgtac
     8821 atatctagta aaaagagaaa cagataaaaa tggagaactg actgaaaata aattgagttc
     8881 atagattccc cagatctcgg tagaaaacaa ataatcatga aggacttttc cttcctctgc
     8941 tgttaactct accctttcat ccatgctctt catataagac ttgatcataa tggcatttag
     9001 aatatgtttc tgtttgttgt gagaatgggc atgcttttga tactccctgc gatataagtc
     9061 ctcaagaggt gctacattct ttggttccaa gacatctgta atttcttttc tcaactcaga
     9121 atctgtatca tactggaaac ctctcgccag aaagaggatc tcctccacac tggcagatat
     9181 attttccaga gcaaatagaa acttttccac cgaaagctca ctctgacctg tttcaaaacg
     9241 ggacaacata gacggcgaaa attgtcctcc ggttgcttgt ctcagtgaga tatttcttga
     9301 ctctcgtaat tgtctaaaga cttttccaat ctgctccata gacttcccct tgattccgta
     9361 ttttcttcat tttatcatat ttttcagaaa attcatcaaa aacttgccaa attgtcagaa
     9421 ttatgagaaa atagaggata tttatcacgt ggagggactg ctatgagaga cgatatcaaa
     9481 atcaatgacc gtgctttggc cttgcaagac caaatcatcg aaaaactaga gaaagttttt
     9541 gatacagatg tggaattgga tgtttacaat ctaggtctga tttatgaaat caatctggat
     9601 gaaacggggc tctgcaagat tgtcatgacc ttcaccgata ctgcctgtga ttgcgccgaa
     9661 agcctgccta ttgaaatcgt ggcaggtctg aaacaaatcg agggtatcaa agatatcaag
     9721 gttgaagtta cctggtcgcc tgcttggaaa atcacacgaa tcagtcgcta tggccgtatt
     9781 gcccttggac taccacctcg ttaagcaggc caatcatttt taaagatgaa aaaagcaagc
     9841 caaaaatggc ttgctttttg atactcaatg aaaatcaaag ggcaaactag aaaactagcc
     9901 gcaggttgct caaaacactg ttttgaagtt atggata
//
LOCUS       DAVLSL010000072         9831 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLSL010000072 DAVLSL010000000
VERSION     DAVLSL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.073"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            157..5067
                     /locus_tag="VXN30_001335"
     CDS             157..5067
                     /locus_tag="VXN30_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1357951.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTSAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQKPKKNYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKIGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHEISTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLDAKPNHPSGSALAPLLEKNK
                     VLVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNTVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            5157..6353
                     /locus_tag="VXN30_001336"
     CDS             5157..6353
                     /locus_tag="VXN30_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1357952.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6489..7016
                     /locus_tag="VXN30_001337"
     CDS             6489..7016
                     /locus_tag="VXN30_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1357953.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7093..7449
                     /gene="glnR"
                     /locus_tag="VXN30_001338"
     CDS             7093..7449
                     /gene="glnR"
                     /locus_tag="VXN30_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1357954.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7486..8832
                     /gene="glnA"
                     /locus_tag="VXN30_001339"
     CDS             7486..8832
                     /gene="glnA"
                     /locus_tag="VXN30_001339"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1357955.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8928..9047)
                     /locus_tag="VXN30_001340"
     CDS             complement(8928..9047)
                     /locus_tag="VXN30_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1357956.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9305..9442
                     /locus_tag="VXN30_001341"
     CDS             9305..9442
                     /locus_tag="VXN30_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357957.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
     gene            9535..9831
                     /locus_tag="VXN30_001342"
                     /pseudo
     CDS             9535..9831
                     /locus_tag="VXN30_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3171 a         1782 c         2142 g         2736 t
ORIGIN      
        1 aaccttattt tagatttgtg caaacagtta ttgttttttg tctaaaaatg ttttaggata
       61 aaaatgtgat tatcatggtt ctagagtttg ataaaaaact acagaatata aagcgctttc
      121 tttaaaacaa caaaatcaat cttttaggag gagaaaatga agaatccatt ttttgaaaga
      181 cgttgtcgtt acagtattcg taagttatca gtaggagcct gctcgctgat gattggtgct
      241 gttttatttg ctggtccagc cttggctgaa gaaactgcag ttcctgaaaa tagcggagct
      301 aatacagagc ttgtttcagg agagagtgag cattcgacca atgaagctga taagcagaat
      361 gaaggggaac atgctagaga aaacaagcta gaaaaggcag aagcagctag cgcagctaaa
      421 ccagaggaaa aagcaggtga ggtggttgca gaaacaccat ctgcagaagc aaaacctaag
      481 tctgacaagg aaacagaagc aaagcccgaa gcaactaacc aaggggatga gtctaagcca
      541 gcagcagaag ctaataagac tgaaaaagaa gtccagccag atgtccctaa aaatacagaa
      601 aaaacattaa aaccaaagga aatcaaattt aattcttggg aagaattgtt aaaatgggaa
      661 ccaggtgctc gtgaagatga tgctattaac cgcggatctg ttgtcctcgc ttcacgtcgg
      721 acaggtcatt tagtcaatga aaaagctagc aaggaagcaa aagttcaagc cttatcaaac
      781 accaattcta aagcaaaaga ccatgcttct gttggtggag aagagttcaa ggcctatgct
      841 tttgactatt ggcaatatct agattcaatg gtcttctggg aaggtctcgt accaactcct
      901 gacgttattg atgcaggtca ccgtaacggg gttcctgtat acggtacact cttcttcaac
      961 tggtctaata gtattgcaga tcaagaaaga tttgctgaag ctttgaagca agacgcagat
     1021 ggtagcttcc caattgcccg taaattggta gacatggcca agtattatgg ctatgatggc
     1081 tatttcatca accaagaaac aactggagat ttggttaaac ctcttggaga aaagatgcgc
     1141 cagtttatgc tctatagcaa ggaatatgct gctaaggtaa accatccaat caagtattct
     1201 tggtacgatg ccatgaccta taactatgga cgttaccatc aagatggttt gggagaatac
     1261 aactaccaat tcatgcaacc agaaggagat aaggttccgg cagataactt ctttgctaac
     1321 tttaactggg ataaggctaa aaatgattac actattgcaa ctgccaactg gattggtcgt
     1381 aatccttatg atgtatttgc aggtttggaa ttgcaacagg gtggttccta caagacaaag
     1441 gttaagtgga atgacatttt agacgaaaat gggaaattgc gcctttctct tggtttattt
     1501 gccccagata ccattacaag tttaggaaaa actggtgaag attatcataa aaatgaagat
     1561 atcttcttta caggttatca aggagaccct actggccaaa aaccaggtga caaagattgg
     1621 tatggtattg ctaacctagt tgcggaccgt acgtcagcgg taggtaatac ttttactact
     1681 tcttttaata caggtcatgg taaaaaatgg ttcgtagatg gtaaggtttc taaggattct
     1741 gagtggaatt atcgttcagt atcaggtgtt cttccaacat ggcgctggtg gcagacttca
     1801 acaggggaaa aacttcgtgc agaatatgat tttacagatg cctataatgg cggaaattcc
     1861 cttaaattct ctggtgatgt agccggtaag acagatcagg atgtgagact ttattctact
     1921 aagttagaag taactgagaa gaccaaactt cgtgttgccc acaagggagg aaaaggttct
     1981 aaagtttata tggcattctc tacaactcca gactacaaat tcgatgatgc agatgcatgg
     2041 aaagagctaa ccctttctga caactggaca aatgaagaat ttgatctcag ctcactagca
     2101 ggtaaaacca tctatgcagt caaactattt ttcgagcatg aaggtgctgt aaaagattat
     2161 cagttcaacc tcggacaatt aactatctcg gacaatcacc aagagccaca atcgccgaca
     2221 agcttttctg tagtgaaaca atctcttaaa aatgcccaag aagcggaagc agttgtgcaa
     2281 tttaaaggca acaaggatgc agatttctat gaagtttatg aaaaagatgg agacagctgg
     2341 aaattactaa ctggctcatc ttctacaact atttatctac caaaagttag ccgctcagca
     2401 agtgctcagg gtacaactca agaactgaag gttgtagcag tcggtaaaaa tggagttcgt
     2461 tcagaagctg caaccacaac ctttgagtgg gggatgactg tccaagacac aagtcttcca
     2521 agacctctag cagaaaacat cgttccaggg gctactgtta tcggaagtac cttcccagat
     2581 acagaaggtg gagaaggtat tgaaggtatg ttgaacggta ccattactag cttgtcagat
     2641 aaatggtctt cagctcagtt gagtggtagt gtggatattc gtttgaccaa gccacgtacc
     2701 gttgttagat gggtcatgga tcatgcaggg gctggtggtg agtctgttaa cgatggcttg
     2761 atgaacacca aagactttga cctttattat aaagatgcag atggtgagtg gaagctagct
     2821 aaggaagtcc gtggcaacaa agcacacgtg acagatatca ctcttgataa accaatcact
     2881 gctcaagact ggcgcttgaa tgttgtcact tctgacaatg gaactccatg gaaggctatt
     2941 cgtatctata actggaaaat gtatgaaaag cttgatactg agagtgtcaa tattccgatg
     3001 gccaaggctg cagcccgttc tctaggcaat aacaaggtac aagttggctt tgcagatgta
     3061 ccggctggag caactattac cgtttatgat aatccaaatt ctcaaactcc gcttgcaacc
     3121 ttgaagagcg aagttggagg agacctagca agtgcaccat tggatttgac aaatcaatct
     3181 ggtcttcttt attatcgtac ccagttgcca ggcaaggaaa ttagtaatgt cctagcagtt
     3241 tccgttccaa aagatgacag aagaatcaag tcagtcagcc tagaaacagg acctaagaaa
     3301 acaagctacg ccgaagggga ggatttggac cttagaggtg gtgttcttcg agttcagtat
     3361 gaaggaggaa ctgaggacga actcattcgc ctaactcacg caggtgtatc agtatcaggt
     3421 tttgatacgc atcataaggg agaacagaat cttactctcc aatatttggg acaaccagta
     3481 aatgctaatt tgtcagtgac tgtcactggc caagacgaag caagtccgaa aactattttg
     3541 ggaattgaag taagtcagaa accgaaaaaa aattacctag ttggtgatag cttagacttg
     3601 tctgaaggac gctttgcagt ggcttatagc aatgacacca tggaagaaca ttcctttact
     3661 gatgagggag ttgaaatttc tggttacgat gctcaaaaga ttggtcgtca aaccttgacg
     3721 cttcgttacc aaggtcatga agtcaacttt gatgttttgg tatctccaaa agcagcattg
     3781 aacgatgagt acctcaaaca aaaattagca gaagttgaag ctgctaagaa caaggtggtc
     3841 tataactttg cttcaccaga agtaaaagaa gctttcttga aagcaattga agcggccgaa
     3901 caagtgttga aagaccatga aattagcacc caagatcaag tcaatgaccg acttaataaa
     3961 ttgacagaag ctcataaagc tctgaatggt caagagaaat ttaaggaaga aaagacagag
     4021 cttgatcgtt taacaggtga ggttcaagaa ctcttggatg ccaaaccaaa ccatccttca
     4081 ggttctgccc tagctccgct tcttgagaaa aacaaggtct tggttgaaaa agtagatttg
     4141 agtccagaag agcttgcaac agcgaaacag agtctaaaag atctggttgc tttattgaaa
     4201 gaagacaagc cagcagtctt ttctgatagt aaaacaggtg ttgaagtaca cttctcaaat
     4261 aaagagaaga ctgtcatcaa gggtttgaaa gtagagcgtg ttcaagcaag tgctgaagag
     4321 aagaaatact ttgctggaga agatgctcat gtctttgaaa tagaaggttt ggatgaaaaa
     4381 ggtcaagatg ttgatctctc ttatgcttct attgtgaaaa tcccaattga aaaagataag
     4441 aaagttaaga aagtattttt cttacctgaa ggcaaagagg cagtagaatt ggcttttgaa
     4501 caaacggata gtcatgttat ctttacagca cctcacttta ctcattatgc ctttgtttat
     4561 gaatctgctg aaaaaccaca acctgctaaa ccagcaccac aaaacacagt ccttccaaaa
     4621 cctacttatc aaccggcttc tgatcaacaa aaggctccta aattggaagt tcaagaggaa
     4681 aaggttgcct ttcatcgtca agagcatgaa aatgctgaga tgctagttgg ggaacaacga
     4741 gtcatcatac agggacgaga tggactgtta agacatgtct ttgaagttga tgaaaacggt
     4801 cagcgtcgtc ttcgttcaac agaagtcatc caagaagcga ttccagaaat tgttgaaatt
     4861 ggaacaaaag taaaaacagt accagcagta gtagctacac aggaaaaacc agctcaaaat
     4921 acagcagtta aatcagaaga agcaagcaaa caattgccaa atacaggaac agctgatgct
     4981 aatgaagccc taatagcagg cttagccagc cttggtcttg ctagtttagc cttgaccttg
     5041 agacggaaaa gagaagataa agattaaata tcgaaaaatc ttgtgaaatc tttccttata
     5101 tttctaaagt gtgatataat agtttcgaat aaaataaata aaggagtttt tgtaacatgg
     5161 caaaacttac tgttaaagac gttgacttga aaggtaaaaa agtcctcgtt cgtgttgact
     5221 tcaacgtacc attgaaagat ggcgtaatca ctaacgataa ccgtatcaca gcagctcttc
     5281 caactattaa gtacatcatc gaacaaggtg gacgtgcaat tcttttctct caccttggac
     5341 gtgtgaaaga agaagctgat aaagctggta aatcacttgc tcctgtagca gctgacttgg
     5401 cagcaaaact tggtcaagat gttgttttcc caggtgtcac tcgtggtgct gaattggaag
     5461 cggcaatcaa cgctcttgaa gatggacaag ttctcttggt tgaaaacact cgttacgaag
     5521 atgttgacgg caagaaagaa tctaaaaacg atcctgaact tggtaaatac tgggcatcac
     5581 ttggagatgg tatcttcgta aacgatgcat tcggtacagc tcaccgtgca cacgcatcta
     5641 acgttggtat ctcagcaaac gttgaaaaag cagttgctgg tttccttctt gaaaacgaaa
     5701 ttgcctacat ccaagaagca gttgaaactc cagaacgtcc attcgtggct atccttggtg
     5761 gttcaaaagt ttcagacaag atcggtgtta tcgaaaactt gcttgaaaaa gctgataaag
     5821 tccttatcgg tggtgggatg acttacacat tctacaaagc acaaggtatc gaaatcggta
     5881 actcacttgt agaagaagac aaattggatg ttgcgaaagc tcttcttgaa aaagcaaatg
     5941 gtaaattgat cttgccagtt gactcaaaag aagctaacgc atttgctggt tacactgaag
     6001 tgcgtgacac tgaaggtgaa gcagtttctg aaggcttcct tggtcttgac atcggtccaa
     6061 aatctatcgc caaatttgac gaagctttga ctggtgccaa aacagttgta tggaacggac
     6121 ctatgggtgt atttgaaaac ccagatttcc aagctggtac aatcggtgtg atggacgcta
     6181 tcgtgaaaca accaggagtt aaatcaatca tcggtggtgg tgactcagct gccgcagcga
     6241 ttaaccttgg ccgtgcagac aagttctcat ggattagtac gggtggtgga gcatcaatgg
     6301 aacttcttga aggtaaggtt cttccaggac ttgcagcctt gacagaaaaa taagatttta
     6361 taaacaaatc aaagaagaga gggatgaaag ttcctctttt cttttgctta aaataaaaac
     6421 gcttcctctc aactattact cataaaaatc accgatttat gataaaatgg aaatagaaag
     6481 ttgagattat gagttatttt aaaaaatata aattcgataa atcccagttc aaacttggta
     6541 tgcgaacctt taaaacaggt attgctgttt ttctagttct cttgattttt ggcttttttg
     6601 gctggaaagg tcttcaaatt ggtgctttga cagccgtttt tagcctgagg gagagttttg
     6661 atgagagtgt tcattttggg acttcgcgta ttctaggaaa tagtatcggt ggactctatg
     6721 ccttggtctt cttcttatta aatacctttt tccacgaagc cttttgggtg accttggtag
     6781 ttgttccaat ctgcaccatg ttaaccatta tgacaaatgt agccatgaat aacaaagcag
     6841 gggttattgg tggtgtagca gctatgttaa tcattaccct atcaattcca agtggtgaga
     6901 caatttcgta cgtgtttgtg cgtgtatcag aaacgtttat gggagttttt gttgcaatta
     6961 tcgtaaatta cgatattgat cgtattcgtc tctttttaga gaaaaaagaa aaataatgtt
     7021 acattttata acattatcaa ttgacgtttg tcttttttta gactataata gacagaaaga
     7081 aggaaattgt aaatgaagga aaaagaattt cgccgaaata tggctgtttt tcctatcggc
     7141 agtgttatga agttgaccga tctatcggcg cgtcagattc gttattatga agatcaagag
     7201 ttgatcaagc ccgatcgaaa cgaaggaaat cgtcgcatgt attccttgaa tgacatggat
     7261 cgtctgcttg aaatcaaaga ttatatctct gaaggttata atatcgctgc cattaagaaa
     7321 aaatatgctg aacgtgaagc gaaatccaag aaagcggtta gtcagactga agtacgtcgt
     7381 gcacttcaca atgaactcct ccaacagggg cgttttgctt cagtacagtc accttttggt
     7441 cgcggttagg caaccgcaag tagtcataga ttaaggagaa aacttatgcc aatcacagct
     7501 gcagatattc gtcgtgaagt caaggaaaaa aatgttacct ttattcgtct tatgttctca
     7561 gatattttgg gaaccatgaa aaacgtcgaa attcctgcta cagatgaaca gttagataag
     7621 gtcttgtcga acaaggttat gtttgatgga tcttctattg aaggttttgt acgtatcaat
     7681 gagtcggata tgtacttgta tccggacttg gatacatgga cagtcttccc ttggggagat
     7741 gaaaatggaa gtgttgcagg tctgatctgt gatgtctata caacagaagg tgaaccattt
     7801 gcgggtgacc ctcgtggtaa tttgaaacga gctcttcgtc acatggaaga agttggattc
     7861 aaatccttca accttggtcc agagccagaa ttcttcctat ttaagttgga tgaaaatggg
     7921 gacccaacac ttgaagtgaa tgacaagggt ggctactttg atttggcacc tactgacctt
     7981 gcggacaaca cacgtcgtga gattgtgaat gtcttgacca aaatgggatt tgaagtagaa
     8041 gcgagtcacc acgaggttgc ggttggacag catgagattg actttaagta cgatgaagtt
     8101 ctccgtgctt gtgataagat tcaaatcttt aagcttgttg ttaaaaccat tgctcgcaaa
     8161 cacggacttt acgcaacatt tatggcgaag ccaaaatttg gtattgctgg atcaggtatg
     8221 cactgtaata tgtccttgtt tgatgcagaa ggaaataacg ccttctttga tccaaatgat
     8281 ccaaaaggaa tgcagttgtc agaaacagct taccatttcc taggcggttt gatcaagcat
     8341 gcttacaact atactgccat catgaaccca acagttaact catacaaacg tttggttcca
     8401 ggttatgaag cgcctgttta cattgcttgg gctggtcgta accgttcgcc acttgtgcgc
     8461 gtacctgctt cacgtggtat gggaactcgt cttgagttgc gttcagtgga tccaatggcg
     8521 aacccttacg ttgctatggc tgttcttttg gaagttggtt tgtatggtat tgaaaataaa
     8581 atcgaagcac cagctcctat cgaagaaaat atctacatca tgacagcaga agagcgcaag
     8641 gaagctggta ttacagacct tccatcaact cttcacaacg ctttgaaagc tttgacagaa
     8701 gatgaagtgg ttaaagctgc tctcggagat cacatctata ctagcttcct tgaagccaaa
     8761 cgaatcgaat gggcaagtta tgcaaccttc gtttcacaat gggaaattga taattattta
     8821 gacctttact aatactaata tagaagaaag attgcctgtg ggtaaacagt cacacagcag
     8881 tctaagccat caaataccta taacccttaa aatagcgatt tcggtcgcta tttatttttc
     8941 gaaaaatcct cttgactctt ttgcgaatta gatttatcat cagacgtgta gaagaaaatc
     9001 aaggagtcac tatggaaaaa ttcaatttca aaaacaatat cgggcaagaa aacaagttac
     9061 ttcaaatcga aatctacaag tttactaact tttgcaagct tcaaaattac acatctgtaa
     9121 atattttttc gaaagatata tttgaagcca tagtaaattg agactagaat agtacactac
     9181 atctgctaaa aatttctaga aattaattta actttcctaa tcgatttgtt tacatcttat
     9241 ttcaatctac tatattatct caataaatga tgatggcgac tgttttgttt gtgcttgtcg
     9301 atttttgaat cctgatcgag cggaagaata tttctgccga ggttgtcaag gggaaaatcc
     9361 tgaagatatt gaattttatg atgaacaatt acaggcagaa aaggtggagg ctttgaatat
     9421 tcgattggaa gtaaaaaatt gaattatcta caatctcatc aacaataaac ctatttcaac
     9481 cccattatct ataggcgtat ttttacgcct ttttatatac ttgaagcaca taagaaacat
     9541 tatgggaagt ttgctgatgg aagcactcaa gaaattgatg ttccttatga tattcctgat
     9601 acttgggagt gggtgaggat aaaatcaatt tattggaatt ttgggcaaaa taagccagag
     9661 aaatccttta ggtatataga tacgtctagt attgatagaa aaaagaacat aatcaactac
     9721 aaaaatctac aatatctttc acctgaacaa gcgccttccc gtgctagaaa attagtttcg
     9781 cagaatagtg tcttattttc aacagttaga ccatatctaa aaaatattgc t
//
LOCUS       DAVLSL010000073         9787 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLSL010000073 DAVLSL010000000
VERSION     DAVLSL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.074"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..251
                     /locus_tag="VXN30_001343"
     CDS             <1..251
                     /locus_tag="VXN30_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1357958.1"
                     /translation="DMATGWLQYNGSWYYLNANGDMATGWVKDGDTWYYLEASGAMKA
                     SQWFKVSDKWYYVNGSGALAVNTTVDGYGVNANGEWVN"
     gene            complement(481..1461)
                     /gene="dusB"
                     /locus_tag="VXN30_001344"
     CDS             complement(481..1461)
                     /gene="dusB"
                     /locus_tag="VXN30_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1357959.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1448..2320)
                     /gene="hslO"
                     /locus_tag="VXN30_001345"
     CDS             complement(1448..2320)
                     /gene="hslO"
                     /locus_tag="VXN30_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1357960.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2485..3912
                     /locus_tag="VXN30_001346"
     CDS             2485..3912
                     /locus_tag="VXN30_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1357961.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKVLDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4071..5579
                     /gene="glpK"
                     /locus_tag="VXN30_001347"
     CDS             4071..5579
                     /gene="glpK"
                     /locus_tag="VXN30_001347"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1357962.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQKRAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5621..7447
                     /gene="glpO"
                     /locus_tag="VXN30_001348"
     CDS             5621..7447
                     /gene="glpO"
                     /locus_tag="VXN30_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1357963.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            7515..8219
                     /locus_tag="VXN30_001349"
     CDS             7515..8219
                     /locus_tag="VXN30_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1357964.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLHWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8516..8719
                     /locus_tag="VXN30_001350"
     CDS             8516..8719
                     /locus_tag="VXN30_001350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357965.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8806..9444)
                     /locus_tag="VXN30_001351"
     CDS             complement(8806..9444)
                     /locus_tag="VXN30_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357966.1"
                     /translation="MCLLVMSVFCISSISPSLVVFADDSLSANSKVVSGEAQFENGSS
                     VRFGDTQVNILSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRS
                     YDPNVWNYVNTIHGNKQANTFANFMTGTGISYMIGRIGALLGGPWGAIIGGAYFGIQA
                     YQSYLDSQSPYPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2838 a         1922 c         2228 g         2799 t
ORIGIN      
        1 gtgatatggc gacaggatgg ctccaataca atggctcatg gtactaccta aacgctaatg
       61 gtgatatggc gacaggttgg gtgaaagatg gagatacctg gtactatctt gaagcatcag
      121 gtgctatgaa agcaagccaa tggttcaaag tatcagataa atggtactat gtcaatggct
      181 caggtgccct tgcagtcaac acaactgtag atggctatgg agtcaatgcc aatggtgaat
      241 gggtaaacta aacctaatat aactagttaa tactgacttc ctgtaagaac tctttaaagt
      301 attccctaca aataccatat cctttcagta gataatatac ccttgtagga agtttagatt
      361 aaaaaataac tctgtaatct ctagccggat ttatagcgct agagactacg gagttttttt
      421 gatgaggaaa gaatggcggc attcaagagg ctctttaaga gagttacggg ttttaaacta
      481 ttaagccttc tccaattgca agagggcttc aatctctgcc agggtgctag cttgcgaaat
      541 ggctccacgg agtttggcag cgccagatgt tccacggagg tagtgaggag cgaggccgcg
      601 gaattcacga actgcgacgt tttctccttt gaggttaatc aatcgtttca agtgttcgta
      661 ggcgatcttc atcttgtctt caaaggtcaa atcaggtagg atttctcctg tttcaaagta
      721 atggttgatt tggttgaaga ggtaaggatt tcccatggca gctcggccaa tcatgactgc
      781 gtcagcacca acttcttcga tgcgttgctt ggcttcttgg acagtacgga tatcaccgtt
      841 ggcgatgaat ggaatcttgg ttagagcttg ggcaaccttg taaagggtct caaggtctgc
      901 gtggccagta tacatttgtt cacgggtacg gccatgcatg gcgagggcag aaacacctgc
      961 agcctcagca gcgagggcat tttctactgc cagagatggg tccgcccagc cggtacgcat
     1021 tttgacagta agtgggatat caaggacaga ctggaccttg ttgatgatag agtagatctt
     1081 gtcaggatcc ttgagccaca tagctccagc ttcgttcttc acgattttgt tgacagggca
     1141 gcccatgttg atatcgacga tatcggtctt ggtgttttct tggatgaatt ctgctgcgcg
     1201 tgctaggctg tcttcatcgc taccaaaaag ttggatagag acagggtttt cgccctcatc
     1261 gatatgaagc atgtgcaggg ttttttcgtt gttgtattgg attcccttgt cagagaccat
     1321 ttccattaca acgagtccag ctccgagctc ttttgcgatg gtacgaaagg ctgagttggt
     1381 cacgccagcc ataggcgcta aaacggtacg attgggaatc tcaatattgc caatcataaa
     1441 aggtgtatta agatttgtca cgaatgagtt cctccaggtc cttttcatca aagttgtaag
     1501 tagtttggca gaattgacaa gtgatttctg ccccgtggtc ttcctctttc atttcctgta
     1561 agtctgagct tggaaggctg gcaagagcgt tcataaagcg ttcatggcta cagtcacatt
     1621 ggaaacggat ttcttcttca gaaagacgct tgtaggcttc gtctccgtag atagccttga
     1681 ggagggcttc gatatggtcg tcgctttcga gaagagtaga gatagctggc atttcttgga
     1741 tgcgtttttc aaagcgagca atctcttctt tcttggctcc tggcaagact tgaactagga
     1801 aaccacctgc aaccttgacc ttgtcttcct cgtccaaaag gacattgagg ccgaccgctg
     1861 aaggcgtttg ttggctttca gtaaggtaaa aggcaaggtc ttcaccgatt tctccagaga
     1921 tgaggggagt tatagagttg taaggatttc cagtaccgta gtctgtgata acgaggaatt
     1981 gaccatttcc aacaaaaggt ccgactagga cttcaccagt cgcagtcttt ttgatgtcaa
     2041 caccaggatt ttgaacatag cctttgacgt tccccttggt atcagcgacg gtgataatag
     2101 cacctagaga gctagatccc aacaccttaa ctgtaagttt ggtatttcct ttttcattgg
     2161 ctgcgagaat ctggctagcg ataagagttc gaccaagcgc tacagttgag ctagcttggg
     2221 tttgatgttt ttcttgagca gtgcggacgg tttcagtgct atcaaggaca aaagcacgaa
     2281 aggctccgct ttctgatata gttttaataa ttttatccat agctactatt ttagcataaa
     2341 aatgcccaaa gggggagccg tgtgtttact gattttcagg ataatggacc aggaaatcag
     2401 catgaaaata aaaagagaaa cagattattt tagcatttgt cagatttatg ctatgcttaa
     2461 ggtagaaaat gaaaggggta acaaatgtat ttaggagatt tgatggagaa agccgagtgt
     2521 ggtcaatttt caatcctttc ctttctatta caagagtctc agacgaccgt caaggctgta
     2581 atggaagaaa caggattttc aaaagcaacc ctaaccaaat atgtcaccct gctcaatgac
     2641 aaggttttgg atagtggctt agagctgact attcactcag aagatgaaaa tctgcgtctg
     2701 tctatcggtg cagctactaa ggggagagat attcggagct tgtttttgga gagtgctgtt
     2761 aaataccaga ttttggttta tcttctctac caccaacagt ttttagccca tcagctggct
     2821 caagaattag tgattagcga ggctacgctt ggtcgtcatt tggctggttt aaatcagatt
     2881 ttgtcagaat ttgatttatc catccaaaat ggacgttggc gaggtccaga gcatcagatt
     2941 cgctatttct atttctgtct tttccgaaag gtctggtcga gtcaggaatg ggaaggtcac
     3001 atgcagaaac cagagagaaa acaggagatt gccaatttag aggaaatctg cggtgcaagt
     3061 ttgtctgcgg ggcagaaatt ggacttggtt ctctgggctc acatcagtca acaacgtctt
     3121 cgggtcaatg cttgtcagtt tcaagtcata gaagagaaaa tgcgagggta ttttgacaat
     3181 atcttctatc ttcgtttgct gagaaaggtt ccgtcctttt ttgctgggca acatattcca
     3241 ctaggagttg aggatggtga gatgatgata ttcttctctt ttctcctatc tcatcgcatt
     3301 cttcctcttc atactatgga atatattctt ggttttggag ggcagttggc agatttactg
     3361 acgcaattga ttcaagaaat gaagaaggag gaactattgg gggattatac agaggaccat
     3421 gtcacctatg aactcagtca gctttgtgct caagtctatc tctataaggg ctatatttta
     3481 caggatcgct acaagtacca gttagagaat cgtcatccat atttactgat ggaacatgat
     3541 tttaaagaga cagcagagga gatttttcat gctctacctg cttttcaaca ggggacagat
     3601 ttagataaga agattctctg ggaatggctc cagttaatcg aatatatggc tgaaaacggt
     3661 ggccagcata tgcggattgg tctggatttg acatctggtt ttcttgtctt ttcaaggatg
     3721 gcagccattt tgaaacggta tttggaatac aatcgtttta ttaccattga agcttatgac
     3781 cctagtcggc attatgattt gctggttacc aataacccga ttcataagaa ggaacagaca
     3841 ccagtctatt atttaaaaaa tgacttggat atggaagatt tggcagggat tcgtcagtta
     3901 ttattcactt aaaaggcttg gttaatccag gtcttttttg tgaaattcac acaatctcct
     3961 cacatttttt taaaaattaa aaaaagttga taaacaagaa agcgctttat tttgtatact
     4021 agtaagtgca aagaggaaat acctcaagat ctttatcagg aggacagtac atgtcacaag
     4081 aaaaatacat catggccatt gaccagggaa ctacaagttc tcgtgccatc attttcaaca
     4141 aaaaagggga aaaggttagc tcgagtcaaa aagagtttac ccagattttc cctcaggcag
     4201 gttgggttga gcacaatgcc aatgaaattt ggaactctgt tcagtcagtt attgcgggtg
     4261 ctttcatcga aagtggtgtc aagccaaatc aaatcgaggc aatcgggatt accaaccaac
     4321 gtgaaacaac ggttgtctgg gataagaaaa caggacttcc tatctacaat gctatcgttt
     4381 ggcagtcacg ccagacagca cctttggctg agcaactaaa aagccaaggt tatgtggaaa
     4441 aattccatga aaagactggt ttgattattg atgcttactt ctctgctacc aaggttcgtt
     4501 ggattttgga tcatgtagaa ggtgctcaaa agcgagcaga aaaaggggaa ttgctctttg
     4561 gtactatcga tacttggttg gtttggaaat tgactgacgg tgcggctcac gtgactgact
     4621 actcaaatgc agctcgtacc atgctttata acattaaaga actcaaatgg gatgatgaga
     4681 ttttggaaat ccttaacatt ccgaaggcta tacttccaga agttcgttct aactccgaaa
     4741 tctacggcaa gacagctcca ttccatttct acggtggaga ggtgccaatc tcaggtatgg
     4801 ctggggacca acaagcagcc ctctttggac agttggcttt tgagccaggt atggttaaga
     4861 atacttatgg aacaggctct ttcatcatca tgaatactgg ggaagagatg cagttgtctg
     4921 aaaacaacct cttgacaacc attggttacg gaatcaacgg taaggtttat tatgccttgg
     4981 aaggttctat cttcatcgca ggaagtgcta ttcagtggct tcgtgacggt cttcgcatgg
     5041 ttgaaaattc accagaatct gaaaaatacg ctcgtgattc tcacaacaac gatgaagttt
     5101 atgtcgttcc agcctttaca ggtctaggcg ctccatactg gaaccaaaat gctcgtggtt
     5161 ccgtctttgg tttgactcgt ggaacaagca aagaagactt tatcaaggcg actttgcaat
     5221 ctattgctta tcaagtgcgt gacatcatcg acaccatgca agtggatact cagaccgcca
     5281 ttcaagtact gaaggtggat ggtggtgcag ccatgaacaa cttcctcatg cagttccagg
     5341 cggatatttt aggcattgac attgcacgtg ctaaaaactt ggaaacaaca gctctaggag
     5401 cggccttcct agcaggtttg tcagtagggt actggaaaga cttggacgag ttgaaactct
     5461 tgaacgagac aggagaactc tttgagccat ctatgaatga atctcgcaag gaacaactct
     5521 acaagggctg gaagaaggct gtgaaagcaa ctcaagtctt tgcggaagta gacgactaat
     5581 actggcagaa taaagcgatt tatttagaaa gtgtgtaaat atggaatttt caaagaaaac
     5641 acgtgaattg tcaattaaaa aaatgcagga acgcaccctg gacctcttga ttatcggtgg
     5701 aggaatcaca ggagctggtg tagccttgca ggcggcagct agcggtcttg agactggttt
     5761 gattgaaatg caagactttg cagaaggaac atctagtcgt tcaacaaaat tggttcacgg
     5821 aggacttcgt tacctcaaac aatttgacgt agaggtggtc tcagatacgg tttctgaacg
     5881 tgcagtggtt caacaaatcg ctccacacat tccaaaacca gatccaatgc tcttaccagt
     5941 ttacgatgaa gatggagcaa cctttagcct cttccgtctt aaagtagcca tggacttgta
     6001 cgacctttta gcaggtgtga gcaacacacc aactgcgaac aaggttttga gcaaggatca
     6061 agtcttggaa cgccagccaa acttgaagaa ggaaggcttg gtaggaggtg gagtgtatct
     6121 tgacttccgt aacaacgatg cgcgtctcgt gattgaaaac atcaaacgtg caaaccaaga
     6181 cggtgccctc attgccaacc acgtgaaggc agaaggcttc ctctttgacg aaagtggcaa
     6241 gattacaggt gttgtagctc gtgatctctt gacagaccaa gtatttgaaa tcaaggcccg
     6301 tctggttatt aatacaacag gtccttggag tgataaagta cgtaatttgt ctaataaggg
     6361 aacgcaattc tcacaaatgc gcccaactaa gggagttcac ttggtagtgg attcaagcaa
     6421 aatcaaggtt tcacagccag tttactttga cacaggtttg ggtgacggtc gtatggtctt
     6481 tgttctccca cgtgaaaaca agacttactt tggtacaact gatacagact acacaggtga
     6541 tttggagcat ccaaaagtaa ctcaagaaga tgtagattat ctacttggca ttgtcaacaa
     6601 ccgctttcca gaatccaaca tcaccattga tgatatcgaa agcagctggg caggtcttcg
     6661 tccattgatt gcagggaaca gtgcctctga ctataatggt ggaaataacg gtaccatcag
     6721 tgatgaaagc tttgacaact tgattgcgac tgttgaatct tatctctcca aagaaaaaac
     6781 acgtgaagat gttgagtctg ctgtcagcaa gcttgaaagt agcacatctg agaaatattt
     6841 ggatccatct gcagtttctc gtgggtctag cttggaccgt gatgacaatg gtcttttgac
     6901 tcttgctggt ggtaaaatca cagactaccg taagatggct gaaggagcta tggagcgcgt
     6961 ggttgacatc ctcaaagcag aatttgaccg tagctttaaa ttgatcaatt ctaaaactta
     7021 ccctgtttca ggtggagaat tgaacccagc aaatgtggat tcagaaatcg aagcctttgc
     7081 gcaacttgga gtttcacgtg gtttggatag caaggaagct cattacctag caaatcttta
     7141 tggttcaaat gcaccgaagg tctttgcact tgctcacagc ttggaacaag cgccaggtct
     7201 tagcttagca gacaccttgt ctcttcacta tgcaatgcgc aatgagttga ctcttagccc
     7261 agttgacttc cttcttcgtc gtaccaatca catgctcttt atgcgtgata gcttggatag
     7321 tatcgttgag ccagttttgg atgaaatggg acgattctat gactggacag aagaagaaaa
     7381 agcaacttac cgtgctgatg taaaagcagc tcttgctcaa aacgacttag cagaattaaa
     7441 aaattaagaa aaaataaaag aggtggaggg tagcaatcct tgtcgcccgc ccctttttta
     7501 atggagacag aaaaatgatg agtgaattat ttggagaatt tttagggact ttaatcctga
     7561 ttcttctagg aaatggtgtt gttgcaggtg tggttcttcc aaaaaccaag agcaacagtg
     7621 caggttggat tgtgattact atgggttggg ggattgcagt tgcggttgca gtatttgtat
     7681 ctggaaaact cagcccagct catttaaatc cagctgtgac agttggtgta gctttaaaag
     7741 gtgacttgca ttgggcttcc gttttgcctt atatcttagc ccagttcgca ggggccatgc
     7801 tgggtcagat tctggtttgg ttgcaattca aaccgcatta tgaggtagaa gaaaatgcag
     7861 gcaatatcct ggcaaccttc agtactggac cagccatcaa ggatactgta tcaaacttga
     7921 ttagcgaaat ccttggaacc tttgttttgg tgttgacaat ctttgctttg ggtctttacg
     7981 attttcaggc aggtatcgga acctttgcag tgggaacttt gattgtcggt atcggtctat
     8041 cactaggtgg gacaacaggt tatgccttga acccagctcg tgaccttgga cctcgtatca
     8101 tgcacagcat cttgccaatt ccaaacaagg gagacggaga ctggtcttac gcttggattc
     8161 ctgttgtagg ccctgttatc ggagcagcct tggcagtgct tgtattctca cttttctagt
     8221 ttatactctt cgaaaatcaa attcaaacca cgtcagcgtc gccttaccgt actcaagtac
     8281 agcttgcggc tagcttccta gtttgctctt tgattttcat tgagtattag aaaacaatta
     8341 tgttgataga gcttgggcaa gagcccaatt tcagcaaaaa atgaagtaaa tcttctcata
     8401 ataaaacgca tcatatcaag cacgaaaatt ccacgaggtc aactacagtc agaaagctga
     8461 acaacaagcc aaaacgccca aaaaaggcgg caaaaagcaa gcacctgcaa gcaacgtgcc
     8521 gaaatggtca aatcctgatt atgtcaacga attagaccca aaaatcgttg atatgctagt
     8581 agaatttcac aagtcacaag gcactttgga aactcccgag gcgcaagcag aaatcgccca
     8641 aaaacgtgaa gaaatcgagc aaaggagagc tgagcttgag gataaaaaac aagagctttt
     8701 gaaccgcttg aacaaataga gtttcgcaag tattatgctt acaaattact tgagcaatta
     8761 actaaaatat aaaccctgcc tttatatcta ggcagggttt atattttaga aattcacgta
     8821 ggttgttacg gtttttacat acccagtata gtttgagttt ctatagtatt cagtgataaa
     8881 cttccatttt ctttgagcaa catggatata agtacttgtt atgtagtatg gatatgggct
     8941 ttgtgaatcc aagtaagact gataagcttg tataccaaaa tatgctccac caattattgc
     9001 accccatgga ccccccaata aagcacctat cctaccaatc atataactga ttccagtacc
     9061 agtcatgaag ttagcgaatg tgttagcttg tttattccca tgtattgtgt tgacgtaatt
     9121 ccaaacatta ggatcgtatg atctaaaaga tatatttagg tcgatttcat tcttttgata
     9181 agccatataa aatgccccat tgatatagac gccgtcagca cgtcgttcaa tagtgtctac
     9241 acttccatct ggattgacaa cctcaagaac ttcatcgctt aaaatattta cttgcgtatc
     9301 tccgaaccgc actgatgagc cattctcaaa ctgagcctca ccagatacaa ctttagagtt
     9361 tgccgataag ctatcatcag caaaaacaac aagcgacggg gaaatgctag atatacagaa
     9421 aacagacata actagcaaac acatgcattt aaacatctta gacataacgg aaactccttt
     9481 gtatttttga ttttttttaa cttttattat acaataaaac caaataaaaa gaaagcggta
     9541 acaatatgct taatgcgaaa attttttata tatttttatg tttgatcgtt atcgaaacta
     9601 caggcttgtt gttgttgaaa agaggtctcg aaatgggtta tttagacaca gaagctatta
     9661 tcctcgcagt tttttcattt gctttttaca acctatgttc attcgcttgg gtctgctcta
     9721 caataaaaaa caataaaaaa taaatagacg tattttcaaa aaaaaaaaaa atgcatattt
     9781 atattag
//
LOCUS       DAVLSL010000074         9758 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLSL010000074 DAVLSL010000000
VERSION     DAVLSL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.075"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            266..361
                     /locus_tag="VXN30_001352"
                     /pseudo
     CDS             266..361
                     /locus_tag="VXN30_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(614..1234)
                     /locus_tag="VXN30_001353"
     CDS             complement(614..1234)
                     /locus_tag="VXN30_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1357967.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1234..1716)
                     /locus_tag="VXN30_001354"
     CDS             complement(1234..1716)
                     /locus_tag="VXN30_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1357968.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1716..2558)
                     /locus_tag="VXN30_001355"
     CDS             complement(1716..2558)
                     /locus_tag="VXN30_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1357969.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2653..3453)
                     /locus_tag="VXN30_001356"
     CDS             complement(2653..3453)
                     /locus_tag="VXN30_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1357970.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     TYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3597..4976
                     /gene="glmU"
                     /locus_tag="VXN30_001357"
     CDS             3597..4976
                     /gene="glmU"
                     /locus_tag="VXN30_001357"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1357971.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAEQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4986..5531
                     /locus_tag="VXN30_001358"
     CDS             4986..5531
                     /locus_tag="VXN30_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1357972.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5543..5857
                     /locus_tag="VXN30_001359"
     CDS             5543..5857
                     /locus_tag="VXN30_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1357973.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5874..6566
                     /locus_tag="VXN30_001360"
     CDS             5874..6566
                     /locus_tag="VXN30_001360"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1357974.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6643..7134
                     /gene="rocS"
                     /locus_tag="VXN30_001361"
     CDS             6643..7134
                     /gene="rocS"
                     /locus_tag="VXN30_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1357975.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7232..7822
                     /locus_tag="VXN30_001362"
     CDS             7232..7822
                     /locus_tag="VXN30_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1357976.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7831..9594
                     /locus_tag="VXN30_001363"
     CDS             7831..9594
                     /locus_tag="VXN30_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1357977.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3015 a         1692 c         2077 g         2974 t
ORIGIN      
        1 ccaaaagtta gattttttct gtctaacttt tggggtgtag ttcatgtaca cctgatatga
       61 tgcgttttat aattttaaag actttttgac cagcctcatt ttttaacttg atactcagtg
      121 aaaagcaaag attaaactag gaagctagct gtaggctgct caaagaacag ctttgaggtt
      181 gcagataaaa cttgtgaggt aaccaacata tataatgtga agctgacgtg gtttgaatag
      241 attttaggag ggtatgagtc tggaagtttt aatggataat gcaagattcc atagaatggg
      301 taagctagag ttcttatgtg aagagtttgg gcataaactt ttaccttttc ctccctactc
      361 atcttagtat agaaaagtga atctgaaata gtacataact gcttctaaaa cattcttata
      421 aattgattta aattctcaaa tcatattatt cagttcttat ttcattttgc tctacaatcc
      481 tgttgagaag acacgtgttc atatcaaaaa ggtattggca agttgcaata cctttttaca
      541 aggttctttt gtcttatttt tgtttcaact gactatatct cctatggttc tagttcagaa
      601 ggctaggcta taattatgat tgataagaag tatcattcca agtattgaga gtgaatgttt
      661 caaaatcatg ggtttctata atggtcaggc tggcatttgc tagaccgcca tctttacgaa
      721 gaagtggttc tttatagcct aggagagtac gaagactggc agtaagattg gcgccgtgtc
      781 cgacaattag aatacgttca gctggactat cttttaatga tttgataaat tggatggtcc
      841 gttgagttgt actatagagg gattcggctc cgaacattcg agtgtcaaat tgagcaagat
      901 ttgaacgaaa agcctggatt tgttgcgggt aaatagcttc caaggttgca attttcaaac
      961 cttctaactt cccaagttgc cattcacgga gattaggaac gatttctaaa gaacaggggg
     1021 tatagagttg actttggata atctcagcag atttgaccgc tcgaggtaaa tcacttgaat
     1081 aaatctgatc aaaaggaatt tccttgagat actgaccaag tcgttttagg gtttcaatgg
     1141 attcaggaag aagaggagaa tcaccactag caccttgaaa acgaccttct tggttccaga
     1201 gggtacgacc gtggcggaca aagtagagtt tcattacttg atgtcctcca aaatatctac
     1261 aaagtctgcc tttacaaagc tagccaagtc ttgtggcgcg acgataatgc tgtgtccgac
     1321 ttcgcctgca gagacaatca tttgatccaa atctagagca attttatcga taaaaatggg
     1381 ataattgtgt ttctgacgaa ttccgacagg attattggct ccatgaatgt aaccagttgt
     1441 tttttctaag tccttttgtg gaatcatgct cactttttta ttgccagaaa ttttagctag
     1501 tttcttttca gacaagtgct gagtgatagg gacaattccg ataatcggtc cggtcttgtc
     1561 tcccaaaagc gccaaggttt tgaaaatctg atctcgttca taaccttgag gaagctctcc
     1621 ttctagggca ttgatttgaa tcccctgatg agggatagct gctttagata ggatttgttc
     1681 caccaatgtt tttttgattt taactttttt tgccattatt tatatttatc ctccaattga
     1741 ctcatccaaa taccaagcca gattcccagc gcaaagaaga aggcgatgat gacataaccg
     1801 acaagtgaaa gtcctgtgta ttggatactt tcagcgtttc ctgcatttgg aattaagatc
     1861 aaaagggtac ttgataggac gataccgatg atgaaatgat agacacgtga gtggtagttg
     1921 tttaaggcat aatccatcaa ttttgaaaaa acgatgagag ttgcacctgc accaattcca
     1981 atcggaaaga aggttcccaa gaaatcaaaa gttttaaaac cagtcaacat aggagcatag
     2041 agtcccaaaa tcaaaagtaa atttgatggg ctgaggccag gaaccaagac gccaagggcc
     2101 aatagtgcgc ctgctaggac gaagttaaga aagctggcgc ttaaggttcc aacgacaaaa
     2161 tttaaggcat agagtcctaa tccagaaatg ataaaggttg tccataacca agctaaatca
     2221 atcttgtctc ggtcagattc tcgagttgat tctttgagga ggctaggaac tgtaccgata
     2281 atagctcccg caaagctcca taatacaaaa acctgataat tttcaagcag gtattcaatc
     2341 gggtaggaaa ataagccgat tcccagaagc ataccgatgg caactggaat aaagtacaaa
     2401 acattttctt taaagtcttt aaagggatgg gccagaaagc caatcattcg ttcatagatt
     2461 cctaagattg ctgctagaac ccctccggaa attcccggta ggataaatcc aagagcaatt
     2521 acaatccctt taataacgcg tgctaaccat gagagcatat atttcctcca actatttcta
     2581 tttcaaaaaa tccttactat attgtatcac aatcagacac aaaaagaaaa agcaaatgat
     2641 aaacaaatgc ttttaaggtt ttaaaaaaag ttttcgaaag gtttcttctt tattttttaa
     2701 gggagagata acgttgatat ctaaatcgtg gtcaaagccg gcaatttttc ctttagatgt
     2761 gtattggtga atatcataat ctaaatcagt tttaggactg ctctccaaaa atcctgagtc
     2821 tgagccgtag gacggaatcc aaacagaggt aaacttgcct gtatcaatac tgtgttcttc
     2881 catgaagtag acaccaacgt agatgccgat gtttttagca cctagtgatg ctagttttgc
     2941 tcgaaagttt tcgacacctt cgttcatatt agacatggtt ttgtcttcca cgtcaagcca
     3001 atagtaacta gggctgtaag gagaggcagc attgtagaaa acttcggcag ccttttccat
     3061 ttcttggaca ctttttccag ctacataagc gtagacagca actgggacat tccgcttttg
     3121 aagttcagtg atatgactct tataggtctt gtctattcca ttgataaatg aagcatcatt
     3181 ttcttttgtt gtttgagcac cactgtgaac acgaacaata gcacctgaaa tattttgtga
     3241 gagggcatcg tagttgattt cctcaggacg ctgccagcca gagaggtcaa taatcggttt
     3301 gtctaagtgt ttcaaagcct gtgcttcaat ctgtgctata ttggattttg ttttaaacga
     3361 ttggctgtca ttaagtgggc gattgatgat taaaatgaac atcataatcc caaaaaaact
     3421 aaataaaata agtggatgaa tttgttttct catatcttat aattctaccc taaaaatcaa
     3481 aaaaaatcaa aaaaatgggt taaggaagag actttagagc attttttcat tcaagagtgc
     3541 ggaatgattt gaaatatggt ataataaaag ggaatttcta cagaaaagag aagattatgt
     3601 caaattttgc cattatttta gcagcgggta aagggactcg catgaaatct gatttgccaa
     3661 aagttttgca caaggttgcg ggtatttcta tgttggaaca tgttttccgt agtgtgggag
     3721 ctatccaacc tgaaaagaca gtaacagttg taggacacaa ggcagaattg gttgaggagg
     3781 tcttggctga acagacagaa tttgtgactc aatctgaaca gttgggaact ggtcatgcag
     3841 ttatgatgac agagcctatc ttagaaggtt tgtcaggaca caccttggtc attgcaggag
     3901 atactccttt aatcactggt gaaagcttga aaaacttgat tgatttccat atcaatcata
     3961 aaaatgtggc cactatcttg actgctgaaa cggataatcc ttttggctat ggacgaattg
     4021 ttcgtaatga caatgctgag gttcttcgta ttgttgagca gaaggatgct acagattttg
     4081 aaaagcaaat caaggaaatc aacactggaa catacgtctt tgacaacgag cgtttgtttg
     4141 aggctttgaa aaatatcaat accaataacg ctcaaggcga atactatatt acagacgtca
     4201 ttggtatttt ccgtgaaact ggtgaaaaag ttggcgctta tactttgaaa gattttgatg
     4261 aaagtcttgg ggtaaatgac cgtgtggcgc ttgcgacagc tgagtcagtt atgcgtcgtc
     4321 gcatcaatca taaacacatg gtcaacggtg ttagctttgt caatccagaa gcaacttata
     4381 tcgatattga tgttgagatt gctccggaag ttcaaatcga agccaatgtt atcttgaaag
     4441 ggcaaacgaa aattggtgct gagactgttt tgacaaacgg tacttatgta gtggacagca
     4501 ctatcggagc aggagcggtc attaccaatt ctatgattga ggaaagtagt gttgcagacg
     4561 gtgtgacagt cggtccttat gctcacattc gtccaaattc aagtctgggt gcccaagttc
     4621 atattggtaa ctttgttgag gtgaaaggat cttcaatcgg tgagaatacc aaggctggtc
     4681 atttgactta tatcggaaac tgtgaagtgg gaagcaacgt taatttcggt gctggaacta
     4741 ttacagtcaa ctatgacggc aaaaacaaat acaagacagt cattggagac aatgtctttg
     4801 ttggttcaaa ttcaaccatt attgcaccag tagaacttgg tgacaattcc ctcgttggtg
     4861 ctggttcaac tattactaaa gacgtgccag cagatgctat tgctattggt cgcggtcgtc
     4921 agatcaataa agacgaatat gcaacacgtc ttcctcatca tcctaagaac cagtaggagc
     4981 ctatcatgga gtttgaagaa aaaacgctta gccgaaaaga aatctatcaa ggaccaatat
     5041 ttaaactggt ccaagatcag gttgaattac cagaaggcaa gggaactgcc caacgggatt
     5101 tgattttcca caatggggct gtctgtgttt tagcagtaac ggatgaacaa aaacttatct
     5161 tggtcaagca gtaccgcaaa gctatcgagg cagtctctta cgaaattcca gccggaaaat
     5221 tggaggtagg agaaaacaca gcccctgtcg cagctgccct tcgtgaatta gaggaagaaa
     5281 cagcctatac agggaaatta gaactcttgt acgattttta ttcagctatt ggcttttgta
     5341 atgagaagtt aaaactatat ttagcaagcg atttgacaaa agtggaaaat ccgcgtccgc
     5401 aggatgagga tgaaaccttg gaagtccttg aagtgagctt agaagaagcg aaagaattaa
     5461 tccaatcagg tcatatctgt gatgccaaga caattatggc tgttcagtat tgggagttgc
     5521 agaaaaaata gaggaggtca gtatgggtaa atctttatta acggatgaaa tgattgaaag
     5581 agctaataga ggcgaaaaaa tttcaggtcc tcctttgcta gatgataatg aggagactaa
     5641 gattttacca acctcttctt cccgttttgg ttatgccaat cctaaggatc atggttttag
     5701 ccaggaaacc ttgaaaattc aggtcgaacc atctattcat aaaagccgtc gcattgaaaa
     5761 taccaagaga aatgtcttca attctaagtt gaataaaatc ttattcgcgg tcatctttct
     5821 cttgattttg cttgttttag caatgaaact tttgtaatag aaaaggaatt gaaatgaaaa
     5881 taggaattat tgctgctatg ccagaagaac tggcttatct ggtccagcat ttagataata
     5941 cccaggagca agttgttttg gggaatacct atcatacagg aaccattgct tctcatgaag
     6001 tcgttcttgt agaaagtgga attggtaagg tcatgtctgc tatgagtgtg gcgattttgg
     6061 ctgatcattt ccaggtggat gcccttatta atacgggttc agctggggca gtagcagaag
     6121 gtatcgctgt tggggatgtc gtgattgctg acaaattagc ctatcatgac gtggatgtca
     6181 cagcttttgg ctatgcttat ggacaaatgg cgcaacaacc gctttatttc gaatcagaca
     6241 aaacctttgt tgctcaaatc caagagagtt tatctcaatt ggaccaaaac tggcatcttg
     6301 gtttgattgc tacaggagat agttttgttg caggaaatga caagatagaa gcgattaagt
     6361 cccatttccc agaagtttta gccgtggaga tggagggggc agctattgct caagcagcgc
     6421 atgccctcaa tctcccagtc ttagtcatca gagctatgag tgacaatgcc aaccatgaag
     6481 caaacatctt ttttgatgag tttattatcg aagctggacg tcgctctgcc caagtcttgt
     6541 tgaccttttt gaaggcttta gattaagcgg aaatttgaca gtttttctag cttatgataa
     6601 gatttaagta aagaaaagct agaaaacgtt tcagaggata ttatgagtat tgaaatgacc
     6661 gtcagtgaga ttgcagaggt cttaggatta tctcgccaag caatcaataa ccgtgtcaaa
     6721 gaattaccag aagaagacac agataaaaat gacaaagggg taacagttgt taccagaagt
     6781 ggcttgatta agctagaaga aatctataaa aaaacgattt ttgaagatga gcctgtcagt
     6841 gaagatgtca aacaacgtga actgatggag attctagtgg atgagaagaa tgcagaaatt
     6901 cttcgtcttt atgaacaatt aaaagccaag gatcgtcagt tatcagaaaa agacgagcag
     6961 atgcgtatca aagaccgtca gattgctgaa aaagacaaac aattagatca gcaacaacaa
     7021 ttgacccttc aagccatgaa ggatcaagaa aatcttaagc tagagctgga ccaagcaaaa
     7081 gaagaagtcc aatccactaa gaaaggcttt tttgctcgtt tatttggagg ataatcaagg
     7141 agctgtttag gttaaatgct aacctgatgg cttcttttgt ttgtgaatag tatagttgat
     7201 tagaacagag acagtagagg agaggtacag aatggaacga ttagaagatt acattgcttt
     7261 tgacttagaa tttaatcagc acgaaggaca aacacacttg attcaagtat cggcagtgcg
     7321 ttttagagat ggtcgggaaa tcgatgccta tgattcctat gttcatacca gcgtccctct
     7381 aaaaagcttt atcaatggtt tgaccgggat tacagctgag accttaaaag atgctcctcc
     7441 agttgagaaa gtcttagaag aattccaaga gtttgtgggt tctttgctga tgataggcta
     7501 caatgctgct aaaagtgatt tacctatttt agcagagcat ggtttagact atagccaaca
     7561 gtacaaggtg gatttgtatg atgaagcttt tgaacgtcgg agttcagatt tacatggtat
     7621 tgccaatctc aaattgcaaa ctgttgcctc atttcttgga tttcaaggtc agtctcataa
     7681 tagtttagag gatgctcgga tgacggcgcg tgtttacgaa tccttcttag agtcggatca
     7741 agctagggaa ttgttagaga cagaaagtag cttatcaaac aatccttttg gtggcttgga
     7801 cttgtctcaa ttatttgact aggagtgcct atgaaacctg aaaaacctaa aaaattgggt
     7861 cttaaacaga tttacctcaa tcttgataaa atcctctttc tatttttctt gatttttatg
     7921 atagtggagt atttgtgggt accattcaat tcatggttgg ctagcttctt attagatcag
     7981 acaggttatc tttttatttc ctataacaat gtcttagcca tccttactca ttctccattg
     8041 attagtctag cttttttagc cttgattgta attaaccttc tggttgctta ttttcaaata
     8101 ggactcctct ttattggtgc tcgtcatctc ctctataatg aaaagcgaag gctaattgag
     8161 tatatccgca aggttttcca tgaaagtttt ggatttatga aaaagctaac ttgcgctaaa
     8221 gccttgttta tctttttcta tatagctatg ctttttcctt ttatacggaa aatgctgaag
     8281 atttattatc ttaataaaat cgtcattcca gagtttatcc aagcttattt agaagataaa
     8341 tattggatgt ggtggctgag catcctactt ctatctctta tctttctcta tgtctctgtc
     8401 agattgatgt ttgcccttcc gaagattgta tatgatcagc tgactgttcg agaagcagtg
     8461 atgtttagct tggagaaaac caagaagaga gttacttttt atgcttggaa tttattttac
     8521 atcttgctca aagcacattt attattttat cttcctttga ttcctttatt attggctcaa
     8581 actttggtag atgatatcac tcagagagag tctctgattc ttggtatttt gaattttgtc
     8641 gtgattaaaa atctctatta tatggctctg acttattttt tggttaaatt tgtttcattt
     8701 ttgacaggaa aggagctgga tatgcttcct aggagagaaa aagatcacat cgtgcgatgg
     8761 ggtgtcatga cttgtgccag tcttttcttt gccttagaag gttatattta tctggaggct
     8821 cccatggttc atctacctca acttatttct caccgagggg tttccaatgc aaatgggatt
     8881 caaaatacag tagagtcctt ggaaactaca gcacaactca aaccagactt ggtggagacg
     8941 gacgtgcagg aaacaaaaga tgggcagttt gtcatgatgc atgatgctaa cttgaaaaat
     9001 ctagcaggta tcaataaaag tcctcaagac ttaaacttgg aggagcttaa agggattgat
     9061 atttttgaaa atggctacca gactaaaatt tcaagctttg aagattatct cagtcgagcc
     9121 aacgaacttg gtcaaaaatt actaattgaa attaaaacca gtaaaaaaga tagtccagac
     9181 atgatgaacc gctttttagc ccgttatgct gcaaagctca agatttatgg acatcaaatc
     9241 cagtctttag actaccatgt tgtcgaaaaa gtaagacagt atgatgccga actgccagtt
     9301 tatttcatca tgccctacaa ttctgtcttt cctaaaacaa gagcgacagg atatactatg
     9361 gagtactcaa ccttggatga atattttgta agcaaactat ggacaacgga tcagaaactt
     9421 tatgtctgga ctgtcaatga ttcagaagct atcagcaaat ctcttcactt aggagtagat
     9481 ggagtgatta cagatgattt ggaaaaagtt cagcgggaaa tagaagtagc ccaagaagat
     9541 ccagaatata cggatttgct cttgaaaaaa gcattggaat tctttgaatt ttagtaataa
     9601 aaaagaggaa cggttgttcc tcccatgata ggtaagtatt cttgtgaatg cttgcttttt
     9661 tgtggttgca atccgtttac gagaattgtt tgtattgttc ttgtttcaat ttactttata
     9721 gtgctttgaa gttggaatgc tacactataa cttctaaa
//
LOCUS       DAVLSL010000075         9285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLSL010000075 DAVLSL010000000
VERSION     DAVLSL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.076"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            454..1497
                     /locus_tag="VXN30_001364"
     CDS             454..1497
                     /locus_tag="VXN30_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1357978.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1664..3751
                     /gene="topA"
                     /locus_tag="VXN30_001365"
     CDS             1664..3751
                     /gene="topA"
                     /locus_tag="VXN30_001365"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1357979.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3857..4216
                     /locus_tag="VXN30_001366"
     CDS             3857..4216
                     /locus_tag="VXN30_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1357980.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4309..4941
                     /locus_tag="VXN30_001367"
     CDS             4309..4941
                     /locus_tag="VXN30_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1357981.1"
                     /translation="MIYEFCAENMTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4985..5641
                     /locus_tag="VXN30_001368"
     CDS             4985..5641
                     /locus_tag="VXN30_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1357982.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5660..6292
                     /locus_tag="VXN30_001369"
     CDS             5660..6292
                     /locus_tag="VXN30_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1357983.1"
                     /translation="MNFQLAKYSLLKKFSENIEFTTPEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6320..6744
                     /locus_tag="VXN30_001370"
                     /pseudo
     CDS             6320..6744
                     /locus_tag="VXN30_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
     gene            6756..7793
                     /gene="leuB"
                     /locus_tag="VXN30_001371"
     CDS             6756..7793
                     /gene="leuB"
                     /locus_tag="VXN30_001371"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV1357984.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEYQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVSMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7790..8059
                     /locus_tag="VXN30_001372"
     CDS             7790..8059
                     /locus_tag="VXN30_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1357985.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            8063..8422
                     /gene="leuD"
                     /locus_tag="VXN30_001373"
     CDS             8063..8422
                     /gene="leuD"
                     /locus_tag="VXN30_001373"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1357986.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8641..8784
                     /locus_tag="VXN30_001374"
     CDS             8641..8784
                     /locus_tag="VXN30_001374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357987.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            9045..9206
                     /locus_tag="VXN30_001375"
     CDS             9045..9206
                     /locus_tag="VXN30_001375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1357988.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
BASE COUNT         2858 a         1635 c         2063 g         2729 t
ORIGIN      
        1 tttagtgtaa ttgagaaagg agaaatgatt gtgattgatg ttggctaggt tatgttcaat
       61 gattcctacc gtctcaaatc ttgtcagtaa ggaaaaataa attcttcaaa agtagagatt
      121 acaaggcttg tttaagaaag aattcaaaga ccttgacaaa taaaaataaa atggttatta
      181 taaaaaatgg tctaaaatag atgatgatac ttttcgaaaa tctttttacg tcagctcagc
      241 tttgccttgc tgtgttttga gcaagctacg gttagcttcc gagtttgatt ttcatttact
      301 agaaatgaaa ctgatgagag atatcagtag acatttgagt caggatatta tggaaaatga
      361 taaaaagagc tcgtgagatt ggcatatcag actactaaag tattgagttt gttaggattt
      421 tagcgactag ttagctggga aaggaagata tttgtgacaa ataataaact gtattcgttg
      481 atagaattta gaaataaaat atatgaagaa ttagaacttt ccagaagtga tttagcgatt
      541 ttactatgtg ccatgcttat cgcctctatc ggattaaata tggattcgac tcccgtgatt
      601 attggagcca tgttaatctc tcctttgatg acacctattc tgggagtggg gctctctcta
      661 gctatatttg attttaaatt gttaagaaaa tcttttaaaa tattagctat tcaaattctt
      721 gccagtctaa tagcttcaac actttatttt tatctttctc ccatttcgta tgctagttcg
      781 gagattgttg ctagaacctc tccgactatt tgggatgttc tcattgcttt tgtaggaggg
      841 atagcaggta ttattggtgc taggaaaaaa gagaccaata atattgttcc tggtgttgct
      901 attgcaaccg ccttgatgcc tcctctttgt acagtaggtt atgctattgc ttctgctaat
      961 ctaaaattta tcataggctc ctcttaccta ttcctcatca attgtagctt tattgtcatt
     1021 gcgacttata taggtgttag gttgatgatg gttaagaaac attattttaa agataatgaa
     1081 gaagactcta aaatgcgtag gattttgctt ctagttgctg ttttgctgat gattccgagt
     1141 ttcatctctg caacgacttt agtgagagaa acgttgaaaa aagagtccct taagaaattt
     1201 atatcagagc agtttcaggg gtataatatt ttgaaaaaaa cctattctaa aaagactcat
     1261 accctaaagc taaccatttc aggaaattat ttgacagaag aagaactcga tatgatttca
     1321 agtaagagag gtgactatgg tttaagtgat gtttctgttc aagtttcaca attgtctgat
     1381 tcagaacaac ttagtaagga agagctggtg gagtatttct tccagtatat caaggataag
     1441 gaagcaaaag aaaaggagaa agccaataaa tttgatacag agtctgagga gcaataattt
     1501 cttgagaata gctggtttct ctcgtgagtc ttctatgtat atcaaaggaa gactgaggtt
     1561 ttaagtataa aacttttctt ctattatagt agctaagtct tgacagggtt caaaaaatgg
     1621 tttacacttt ataaagttta ttactttgaa aaggtgtgat actgtggcta cggcaacaaa
     1681 aaagaaaaaa tcaacagtta aaaaaaatct agtcatcgtg gagtcgcctg ctaaggccaa
     1741 gacgattgaa aaatatctag gcagaaacta caaggtttta gccagtgtcg ggcatatccg
     1801 tgatttgaag aaatccagta tgtccgtcga tattgaaaat aattatgaac cgcaatatat
     1861 caatatccga ggaaaaggcc ctcttatcaa tgacttgaaa aaagaagcta aaaaagctaa
     1921 taaagttttt ctcgcgagtg acccggaccg tgaaggagaa gcgatttctt ggcatttggc
     1981 ccatattctc aacttggatg aaaatgatgc caaccgtgtg gtcttcaatg aaatcaccaa
     2041 ggatgcagtc aaaaatgctt ttaaagaacc tcgtaagatc gatatggact tggtcgatgc
     2101 ccaacaagct cgtcggatct tggatcgctt ggtagggtat tcgatttcgc ctattttgtg
     2161 gaagaaggtc aagaagggct tgtcagcagg tcgcgttcag tccattgccc ttaaactcat
     2221 cattgaccgt gaaaatgaaa tcaatgcctt ccagccagaa gaatactgga cagttgatgc
     2281 tgtctttaaa aagggaacca aacaatttca tgcttccttc tatggagtag atggtaaaaa
     2341 gatgaaactg accagcaatg acgaagtcaa ggaagtcttg tctcgtctga ctagtaaaga
     2401 cttttcagta gatcaggtgg ataagaaaga gcgcaagcgc aatgctcctt taccctatac
     2461 cacttcatct atgcagatgg atgctgccaa taaaatcaat ttccgtactc gaaaaaccat
     2521 gatggttgcc caacagctct atgaaggaat taatattggt tctggtgttc aaggtttgat
     2581 tacctatatg cgtaccgatt cgactcgtat cagtcctgta gcgcaaaatg aggcggcaag
     2641 cttcattacg gatcgttttg gtagcaagta ttctaagcac ggtagcaagg tcaaaaacgc
     2701 atcaggtgct caggatgccc atgaggctat tcgtccgtca agtgtcttta atacaccaga
     2761 aagcatcgct aagtatctgg acaaggatca gctcaagcta tataccctta tctggaatcg
     2821 ttttgtggct agccagatga cagcggccgt ttttgatacc atggctgtta aattgtctca
     2881 aaatggagtt caatttgctg ccaatggtag tcaggttaag tttgatggtt atcttgccat
     2941 ttataatgat tctgacaaga ataagatgtt accggacatg gttgttggag atgtggtcaa
     3001 acaggtcaat agcaaaccag agcaacattt cacccaaccg cctgcccgtt attctgaagc
     3061 aacactgatt aaaaccttag aggaaaatgg ggttggacgt ccatcaacct acgcgccaac
     3121 cattgaaacc attcagaaac gttattatgt tcgcctggca gccaaacgtt ttgaaccgac
     3181 agagttggga gaaattgtca ataagctcat cgttgaatat ttcccagata tcgtaaacgt
     3241 gaccttcaca gctgaaatgg aaggtaaact ggatgatgtc gaagttggaa aagagcagtg
     3301 gcgacgggtc attgatgcct tttacaaacc attctctaaa gaagttgcca aggctgaaga
     3361 agaaatggaa aaaatccaga ttaaggatga accagctgga tttgactgtg aagtgtgtgg
     3421 cagtccaatg gtcattaaac ttggtcgttt tggtaaattc tacgcttgta gcaatttccc
     3481 agattgccgt catacccaag caatcgtgaa agagattggt gttgagtgtc caagctgtca
     3541 tcagggacaa attattgagc gaaaaaccaa gcgtaatcgc ctattctatg gttgcaatcg
     3601 ctatccagaa tgtgaattta cctcttggga caagcctgtt ggtcgtgact gtccaaaatg
     3661 tggcaacttc ctcatggaga aaaaagtccg tggtggtggc aagcaggttg tttgtagcaa
     3721 aggcgactac gaggaagaaa agattaaata agaggagggg cccgaaagta aatttcaggc
     3781 tccttttggt tagaacttga caaaatacat cattttatgc gaaactagaa gaagattatt
     3841 ttaactagga gaagttatgc gtcttatcta tctgataatt ggttttttat cactggcctt
     3901 ggctattgtt ggggttgttt tacccttgtt gccgacaacg cctttccttt tgttgtctat
     3961 tgcttgtttc tccagaagtt ccaagcgttt cgaagactgg ctttatcata ccaagctcta
     4021 tcaaacatat gtagctgatt ttcgcgagac taagtctatt acgcgtgaac gaaagaaaaa
     4081 aatcatcgtc tctatctacg tcttgatggg aatttctatt tattttgcac ctcttttacc
     4141 agtcaaaatc ggtctgggtg ctttgaccat ctttatcact tattatctct tcaaggtcat
     4201 tccagacaaa gaatagttaa aacagtagtt atttgccttg ataaaattga aagcatattc
     4261 ataacaatat gatataataa atttgaagta atattcaagg agaatcaaat gatttacgaa
     4321 ttttgtgctg aaaatatgac tttacttgaa aaagcgatgc aggctggagc tcgtcggatt
     4381 gaactctgtg ataatctagc agttggtggg acaacaccca gctatggagt gactaaggca
     4441 gcggttgaac tggcagctaa ctacgataca accatcatga ccatgattcg gccacgtggt
     4501 ggtgactttg tctataatga cctagaaatt gctatcatgc tagaagacat tcgtttgact
     4561 gctcaggctg gaagtcaagg ggttgtattt ggagctttaa ctgctgataa aaagttggat
     4621 aagcctaatc tggaaaagtt aattgctgca tcaaaaggaa tggaaattgt ctttcacatg
     4681 gcctttgatg aactaagtga tgaagatcaa ccggaagcta ttgactggct cagtcaagcc
     4741 ggtgtcactc gtatcctaac tcgtgctggt gtgtctggcg actccttaga aaaacgtttt
     4801 gttcactatc acagaatttt ggagtacgct aaaggtaaaa ttgaaattct accaggtggg
     4861 gggattgacc ttgaaaaccg tcaaaccttt atcgaccagg tgggggtaac acaattgcat
     4921 ggtactaagg ttgtttttta aaaaatagaa aggaactgct agcttttggt agcagttttc
     4981 acttatgttt gaaattttta aatcctatca atttaatcaa gaaaaggctc atgattatgg
     5041 ttttatagaa aatagcgaag tctggacata tagttgccag attttgcaag gtgactttgt
     5101 catgactgtg tccatcactg ctgataatgt gaactttcaa gtctttgacc aagagactgg
     5161 tgacctctat cctcacgttc atatggaaag catgagggga agttttgtcg gaaatgtccg
     5221 tgaggcttgt ctggagattc tttaccagat tcggaaggct tgttttgatg tgcaagattt
     5281 tatctgtcat cagactaagc gtatcatgac tcaagttcag gaaaagtatg gaaaccagtt
     5341 ggagtatctg tgggaaaaat cgcctgatac agctgtattg cgccatgaag gcaatcaaaa
     5401 gtggtatgcc gtcttgatga aaatctcttg gaataagctg gaaaagggca gagaaggaca
     5461 agtggaagca gtcaacctca agcatgacca agtagctaat ttgctttcac aaaaggggat
     5521 ttatccagcc ttccatatga gcaagcgcta ctggattagt gtgtcccttg atgatacttt
     5581 atcagatgaa gaagtactgg aattgataga aaaaagttgg aacttaacct ctaaaaaatg
     5641 aaatatttta ataattttca tgaactttca attagctaaa tattctttac tgaagaaatt
     5701 ttcagaaaat atagaattta ccacaccaga ggaatatggt gccatcttca aatacctgat
     5761 tgagaatgtc aagacggatc gtcagatcat ctattcgcct cactgtcatg atgaccttgg
     5821 aatggcagtg gcaaatagcc ttgctgctgt caagaatggt gcaggacgtg ttgaagggac
     5881 tatcaatggt attagggagc gagctggaaa tgctgctttg gaagaaattg cagtggctct
     5941 caatattcgc caagattact accaagtaga aaccagtatt gtcctaaatg agaccatcaa
     6001 tacgtcagaa atggtttctc gcttctctgg tattccagtt cctaaaaaca aagccgtcgt
     6061 tggtggcaat accttctccc acgaatctgg tattcaccaa gatggagtcc ttaaaaatcc
     6121 tctcacttat gagatcatca cacctgaatt ggttggtgtt aagattccgc ttggaaaatt
     6181 atctggtcgc catgcttttg ttgagaaact gagagaattg gccctagatt ttacagaaga
     6241 ggatatcaaa ccactctttg ctaagttcaa ggcactggcc gataagaagt aagaaatcac
     6301 agatgcagat actcgagctt tggtagctgg aaccatggtt gaaaatccag aaggcttcca
     6361 ctttgatgat ttacaacttc aaactcatgc agataatgac attgaagcgc tcgttagcct
     6421 agccaatatg gatggtgaga aagtcgaatt taatgcgaca gggcaaggtt ccgttgaagc
     6481 aatctttaat gctatcgata agttctttaa ccaatctgtt cgtttggtgt cctacactat
     6541 cgatgcggta acagatggaa tcgataccca ggatcgggtt ttggtcactg ttgaaaacag
     6601 agatacagaa accatcttta atgcagcagg gcttgatttt gatgtgttga aggcttctgc
     6661 tattgcctat ataaacgcta atacctttgt tcaaaaagag aatgcaggtg cagtgtttct
     6721 taccacgata tgcctagtgt gtaaaggaga aggctatggc aaagaaaata gtagctctag
     6781 taggagacgg aattggccca gaaatcatgg aggctggttt agaagttctg gaggctctag
     6841 ctgaaaaaac aggttttgac tatgagattg acagacgacc gttcggaggt gcagatattg
     6901 atgcagcagg acctccctta cctgatgaaa cccttaaggc aagtagggaa gcagatgcta
     6961 tcctactagt agctatcggt agtcctcagt atgatggagt agcggttcgc cctgaacaag
     7021 gcctgatggc tctccgtaag gaactcaatc tttacgctaa tattcgtcct gtaaaaatct
     7081 ttgacagtct caagtatttg tcaccactca aaccggaacg aatttctggt gtagactttg
     7141 tcgtggtgcg tgaattgaca ggcgagattt actttggaga tcatattctt gaagagcgca
     7201 aagcgcgtga tatcaacgac tatagctatg aggaagtgga gcggattatt cgcaaagcct
     7261 ttgaaattgc aagaaatcgc agaaaaatcg ttactagtat cgataagcaa aatgttctag
     7321 cgacctcaaa actctggcgg aaagtagctg aggaagtcgc acaggatttc ccagatgtaa
     7381 ccttggaata tcagctggta gactcagctg ctatgcttat gattaccaat cctgctaagt
     7441 ttgatgttat tgtaacggag aatctttttg gagatatttt atctgatgaa tcaagcgtct
     7501 tatctggtac acttggggtt atgccatcag ccagtcattc tgaaaatgga ccaagtctct
     7561 atgaacctat tcacggttca gcacctgata ttgcaggtca aggaattgcc aatcctattt
     7621 ccatgatttt atcagtttcc atgatgttga gagatagttt cggacgttat gaggatgcag
     7681 agcgtatcaa acgtgctgtt gagacaagtc tggcggcagg aattttaacg agagatatag
     7741 gaggtcaggc ttcaacaaag gaaatgacgg aagctattat tgcaaggtta tgaagttaga
     7801 cgaaaaaatt actctagtcc ttttgatttg gaatgtcatc attttcttga tttatggtat
     7861 tgacaaatct aaggcaagga gaagagtttg gcgcatccct gagaaaatct tacttatttt
     7921 agcctttact tttggtggtt ttggtgcctg gctagcagga atcatctttc accacaagac
     7981 tcgaaaatgg tactttaaaa tagtttggtt tcttgggatg gtgaccacac tagtagcctt
     8041 atattttatt tggaggtaat ggatggcagg gtcttcgagg gaatacgctg cttgggctct
     8101 agcggactat ggttttaagg tcgtgattgc aggatctttc ggtgacattc attacaataa
     8161 tgaactcaat aatggcatgt tgccaatcgt tcagcctaga gaggttagag agaaactagc
     8221 ccagctaaaa ccaaccgacc aggtaactgt ggacttggaa caacaaaaaa tcatctcacc
     8281 agttgaagaa ttcaccttcg agatagatag cgagtggaaa cataaactcc taaatagttt
     8341 ggatgatatc ggtattacct tgcagtatga agagttgatt gctgcttatg aaaaacaacg
     8401 accagcctac tggcaggatt agaaaaaata gaaaaggaga tatagtaaac tgaaataaga
     8461 tgtaaacaaa tgaattggag cttaacatcc atttccagca attttttaga aactacagtg
     8521 gactattctg gattcaacac attataaaat tatgacaaaa cacattcaca agaaggctac
     8581 gacattttaa aaggtgaggg cggatgtatc gtttgcccta ctaaagttgg ttacattatc
     8641 atgaccagtg acaaggcagg acttgagcgt aagttcgcag ccaaagaacg taagcgtaac
     8701 aaaccaggtg ttgttctctg cggtagcatg gatgaacttt gcgctttagc gcaactcaac
     8761 ccagaaattg aagcattcta ctaaaaacat tgggatgaag atattcttct tggttgtatc
     8821 cttccttgga aaccagaagc ctttgaaaaa ctcaaagcat acggggatgg ccgtgaagaa
     8881 cttattactg atgtacgtgg tactagctgt tttgttatca agtttggaaa agcaggtgaa
     8941 caattggctg ccaagctttg ggaagaaggt aaaatggtct acgcctcatc tgcttcaatg
     9001 acaaaacgat tgaaactcgc tatgagcaag gtgtaatggt gtctatggtc gataaggacg
     9061 gcaaactcat cccagaacaa ggaggagcac gttcaacttc accagctcca gttgtgatcc
     9121 gtaaagggct tgacattgat aaaatcatga tgcacctgtc agatactttt aactcatggg
     9181 actaccgtca ggttgagtat tattaggata gagaagaagt ctagtgttat gagatattaa
     9241 agctcctaac actgggcttt tgtttagaat ttcttttctt tttct
//
LOCUS       DAVLSL010000076         9219 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLSL010000076 DAVLSL010000000
VERSION     DAVLSL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.077"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..2369
                     /gene="mfd"
                     /locus_tag="VXN30_001376"
     CDS             <1..2369
                     /gene="mfd"
                     /locus_tag="VXN30_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1357989.1"
                     /translation="QFSFLKEEIERYKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLD
                     SRDKTNICKESVNLIEGNLRHGFHFVDEKILLITEHEIFQKKLKRRFRRQHVSNAERL
                     KDYNELEKGDYVVHHIHGIGQYLGIETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLL
                     SKYISSDGKAPKLNKLNDGHFKKAKQKVKNQVEDIADDLIKLYSERSQLKGFAFSADD
                     DDQDAFDDAFPYVETDDQLRSIEEIKRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKA
                     VNDHKQVVILVPTTVLAQQHYTNFKERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQ
                     VDILIGTHRVLSKDVVFADLGLMIIDEEQRFGVKHKETLKELKKQVDVLTLTATPIPR
                     TLHMSMLGIRDLSVIETPPTNRYPVQTYVLEKNDSVIHDAVLREMERGGQVYYLYNKV
                     DTIVQKVSELQELIPEASIGYVHGRMSEVQLENTLLDFIEGQYDILVTTTIIETGVDI
                     PNANTLFIENADHMGLSTLYQLRGRVGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKG
                     FTELGSGFKIAMRDLSIRGAGNLLGKSQSGFIDSVGFELYSQLLEEAIAKRNGNANAN
                     TRTKGNAELILQIDAYLPDTYISDQRHKIEIYKKIRQIDNRVNYEELQEELIDRFGEY
                     PDVVAYLLEIGLVKSYLDKVFVQRVERKDNKITIQFEKVTQRLFLAQDYFKALSVTNL
                     KAGIAENKGLMELVFDVQNKKDYEILEGLLIFGESLLEIKEFKEENSI"
     gene            2427..2693
                     /locus_tag="VXN30_001377"
     CDS             2427..2693
                     /locus_tag="VXN30_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1357990.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            2686..3054
                     /locus_tag="VXN30_001378"
     CDS             2686..3054
                     /locus_tag="VXN30_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1357991.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            3174..4442
                     /locus_tag="VXN30_001379"
     CDS             3174..4442
                     /locus_tag="VXN30_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1357992.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            4439..5716
                     /gene="tilS"
                     /locus_tag="VXN30_001380"
     CDS             4439..5716
                     /gene="tilS"
                     /locus_tag="VXN30_001380"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1357993.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            5720..6262
                     /gene="hpt"
                     /locus_tag="VXN30_001381"
     CDS             5720..6262
                     /gene="hpt"
                     /locus_tag="VXN30_001381"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1357994.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            6278..8236
                     /gene="ftsH"
                     /locus_tag="VXN30_001382"
     CDS             6278..8236
                     /gene="ftsH"
                     /locus_tag="VXN30_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1357995.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            8358..8837
                     /locus_tag="VXN30_001383"
     CDS             8358..8837
                     /locus_tag="VXN30_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1357996.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            8931..9002
                     /locus_tag="VXN30_001384"
     tRNA            8931..9002
                     /locus_tag="VXN30_001384"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8964..8966,aa:Glu,seq:ttc)
BASE COUNT         3209 a         1356 c         1933 g         2721 t
ORIGIN      
        1 atcagttttc ttttctaaaa gaagaaattg aacgatataa aaaaatggat tacaccatta
       61 ttctgcagtc tagcaattca atgggaagta aaacattgga ggatatgtta gaggaatatc
      121 agattaaatt ggattctaga gataagacaa atatctgtaa agaatctgta aacttaatag
      181 agggtaatct cagacatggt tttcattttg tagatgaaaa gattttattg ataactgaac
      241 atgagatttt tcaaaagaaa ttaaagcgtc gttttcgaag acaacatgtt tcaaatgcag
      301 agagattaaa agattacaat gaacttgaaa aaggggacta tgttgtccat catatccatg
      361 ggattggtca atatctagga attgaaacca ttgaaatcaa gggaattcat cgcgattatg
      421 tcagtgtcca ataccaaaat ggtgatcaaa tttctatccc cgtggaacag attcatctac
      481 tgtccaaata tatttcaagt gatggtaaag ctccaaaact caataaatta aatgacggtc
      541 attttaaaaa ggccaagcaa aaggttaaga accaggtaga ggatatagct gatgatttaa
      601 tcaaactcta ctctgaacgt agtcagttga agggttttgc tttctcagct gatgatgatg
      661 atcaagatgc ctttgatgat gctttccctt atgttgaaac ggatgatcaa cttcgtagta
      721 ttgaggaaat caagagggat atgcaggctt ctcagccaat ggatcgactt ttagttgggg
      781 atgttggttt tggaaagact gaagttgcta tgcgtgcagc ctttaaagca gtcaatgatc
      841 acaaacaggt tgtcattcta gttccgacga cggttttagc gcaacagcac tatacgaatt
      901 ttaaggaacg attccaaaat tttgcagtta atattgatgt gttgagtcgc tttagaagta
      961 aaaaagagca gactgcaaca cttgaaaaat tgaaaaacgg tcaagtcgat attttgattg
     1021 gaacacatcg tgttttgtca aaagatgttg tgtttgctga tttgggcttg atgattattg
     1081 atgaggaaca gcgatttggt gtcaagcata aggaaacttt gaaagaactg aagaaacaag
     1141 tggatgtcct aaccttgacc gctacgccaa tccctcgtac cctccatatg tctatgctgg
     1201 gaatcagaga tttatctgtt attgaaactc cgccgactaa tcgctatcct gttcagacct
     1261 atgttttgga aaagaatgat agtgtcattc atgatgctgt cttgcgtgaa atggagcgtg
     1321 gaggtcaagt ttattatctt tacaacaaag ttgacacaat tgttcagaag gtttcagaat
     1381 tacaggagtt gattccggag gcttcgattg gatatgttca tggtcgaatg agtgaagtcc
     1441 agttggaaaa tactctatta gactttattg agggacaata cgatatcttg gtgacgacta
     1501 ctattattga gacaggggtg gacattccaa atgctaatac tttatttatt gaaaatgcgg
     1561 accatatggg cttgtcaacc ttatatcagt taagaggaag agtcggtcgt agtaatcgta
     1621 ttgcttatgc ttatctcatg tatcgtccag aaaaatcaat cagtgaagtc tctgaaaaga
     1681 gattagaagc gattaaagga tttacagaat tgggctctgg ctttaagatt gcaatgcgag
     1741 atctttcgat tcgtggagca ggaaatcttt taggaaaatc ccagtctggt ttcattgatt
     1801 ctgttggttt tgaattgtat tcgcagttat tagaggaagc tattgctaaa cgaaacggta
     1861 atgctaacgc taacacaaga accaaaggga atgctgagtt gattttgcaa attgatgcct
     1921 atcttcctga tacttatatt tctgatcaac gacataagat tgaaatttac aagaaaattc
     1981 gtcaaattga caaccgtgtc aattatgaag agttacaaga ggagttgata gaccgttttg
     2041 gagaataccc agatgtagta gcctatcttt tagagattgg tttggttaaa tcatacttgg
     2101 acaaggtctt tgttcaacgt gtggaaagaa aagataataa aattacaatt caatttgaaa
     2161 aagtcactca acggctgttt ttagctcaag attattttaa agctttatcc gtaacgaact
     2221 taaaagcagg catcgctgag aataagggat taatggagct tgtatttgat gtccaaaata
     2281 agaaagatta tgaaatttta gaaggtctgc tgatttttgg agaaagttta ttagagataa
     2341 aagagtttaa ggaagaaaat tccatttgat atttttcttc tataaaatag ataaaaatgg
     2401 tacaataata aattgaggta ataaggatga gattagataa atatttaaaa gtatcgcgaa
     2461 ttatcaagcg tcgtacagtc gcaaaggaag tagcagataa aggtagaatc aaggttaatg
     2521 gaatcttggc caaaagttca acggacttga aagttaatga ccaagttgaa attcgctttg
     2581 gcaataagtt gctgcttgta aaagtactag agatgaaaga tagtacaaaa aaagaagatg
     2641 cagcaggaat gtatgaaatt atcagtgaaa cacgggtaga agaaaatgtc taaaaatatt
     2701 gtacaattga ataattcttt tattcaaaat gaataccaac gtcgtcgcta cctgatgaaa
     2761 gaacgacaaa aacggaatcg ttttatggga ggggtattga ttttgattat gctattattt
     2821 atcttgccaa cttttaattt agcgcagagt tatcagcaat tactccaaag acgtcagcaa
     2881 ttagcagact tgcaaactca gtatcaaact ttgagtgatg aaaaggataa ggagacagca
     2941 tttgctacca agttgaaaga tgaagattat gctgctaaat atacacgagc gaagtactat
     3001 tattctaagt cgagggaaaa agtttatacg attcctgact tgcttcaaag gtgataaaat
     3061 ggaaaattta ttagacgtaa tagagcaatt tttgagtttg tcagatgaaa agctggaaga
     3121 attggctgat aaaaatcaat tattgcgttt acaagaagaa aaggaaagga agaatgcgta
     3181 aattcttaat tattttgttg ctaccaagtt ttttgaccat ttcaaaagtc gttagcacag
     3241 aaaaagaagt cgtctatact tcgaaagaaa tttattacct ttcacaatct gactttagta
     3301 tttattttag agaaaaatta agttctccca tggtttatgg agaggttcct gtttatgcga
     3361 atgaagattt agtagtggaa tctgggaaat tgactcccaa aacaagtttt caaataaccg
     3421 agtggcgctt aaataaacaa ggaattccag tatttaagct atcaaatcat caatttatag
     3481 ctgcggacaa acgattttta tatgatcaat cagaggtaac tccaacaata aaaaaagcat
     3541 ggttagaatc tgactttaaa ctgtacaata gtccttatga tttaaaagaa gtgaaatcat
     3601 ccttatcagc ttattcgcaa gtatcaatcg acaagaccat gtttgtagaa ggaagagaat
     3661 ttctacatat tgatcaggct ggatgggtag ctaaagaatc aacttctgaa gaagataatc
     3721 ggatgagtaa agttcaagaa atgttatctg aaaaatatca gaaagattct ttctctattt
     3781 atgttaagca actgactact ggaaaagaag ctggtatcaa tcaagatgaa aagatgtatg
     3841 cagccagcgt tttgaaactc tcctatctct attatacgca agaaaaaata aatgagggtc
     3901 tttatcagtt agatacgact gtaaaatacg tatctgcagt caatgatttt ccaggttctt
     3961 ataaaccaga gggaagtggt agtcttccta aaaaagaaga taataaagaa tattctttaa
     4021 aggatttaat tacgaaagta tcaaaagaat ctgataatgt agctcataat ctattgggat
     4081 attacatttc aaaccaatct gatgccacat tcaaatccaa gatgtctgcc attatgggag
     4141 atgattggga tccaaaagaa aaattgattt cttctaagat ggccgggaag tttatggaag
     4201 ctatttataa tcaaaatgga tttgtgctag agtctttgac taaaacagat tttgatagtc
     4261 agcgaattgc caaaggtgtt tctgttaaag tagctcataa aattggagat gcggatgaat
     4321 ttaagcatga tacgggtgtt gtctatgcag attctccatt tattctttct attttcacta
     4381 agaattctga ttatgatacg atttctaaga tagccaagga tgtttatgag gttctaaaat
     4441 gagggaacca gattttttaa atcattttct caagaaggga tatttcaaaa agcatgctaa
     4501 ggcggttcta gctctttctg gtggattaga ttccatgttt ctatttaagg tattgtctac
     4561 ttatcaaaaa gagttagaga ttgaattgat tctagctcat gtgaatcata agcagagaat
     4621 tgaatcagat tgggaagaaa aggaattaag gaagttggct gctgaagcag agcttcctat
     4681 ttatatcagc aatttttcag gagaattttc agaagcgcgt gcacgaaatt ttcgttatga
     4741 tttttttcaa gaggtcatga aaaagacagg tgcgacagct ttagtcactg cccaccatgc
     4801 tgatgatcag gtggaaacga ttttgatgcg cttgattcga ggaactcgct tgcgctatct
     4861 atcaggaatt aaggagaagc aagtagtcgg agagatagaa atcattcgtc ccttcttgca
     4921 ttttcagaaa aaagactttc catcaatttt tcactttgaa gatacatcaa atcaggagaa
     4981 tcattatttt cgaaatcgta ttcgaaattc ttacttacca gaattggaaa aagaaaatcc
     5041 tcgatttagg gatgcaatct taggcatcgg caatgaaatt ttagattatg atttggcaat
     5101 agctgaatta tctaacaata ttaatgtgga aaatttacag cagttatttt cttactctga
     5161 gtctacacaa agagttttac ttcaaactta tctgaatcgt tttccagatt tgaatcttac
     5221 aaaagctcag tttgctgaag ttcagcagat tttaaaatct aaaagccagt atcgtcatcc
     5281 gattaaaaat ggctatgaat tgataaaaga gtaccaacag tttcagattt gtaaaatcag
     5341 tccgcaggct gatgaagagg aagatgaatt tgtgttacac tatcaaaatc aggtagctta
     5401 tcaaggatat ttattttcct ttggacttcc attagaaggt gaattaattc aacaaatacc
     5461 tgtttcacgt gaaacatcca tacacattcg tcatcgaaaa acaggagatg ttttgattca
     5521 aaatgggcat agaaaaaaac tcagacgttt atttattgat ttgaaaatcc ctatggaaaa
     5581 gagaaactct gctcttatta ttgagcaatt tggtgaaatt gtctcaattt tgggaattgc
     5641 gaccaataat ttgagtaaaa aaacgaaaaa tgatataatg aacactgtac tttatataga
     5701 aaaaatagat aggtaaaaaa tgttagaaaa cgatattaaa aaagtcctcg tttcacacga
     5761 tgaaattaca gaagcagcta aaaaactagg tgctcaatta actaaagact atgcaggaaa
     5821 aaatccaatc ttagttggga ttttaaaagg atctattcct tttatggctg aattggtcaa
     5881 acatattgat acacatattg aaatggactt catgatggtt tctagctacc atggtggaac
     5941 agcaagtagt ggtgttatca atattaaaca agatgtgact caagatatca aaggaagaca
     6001 tgttctattt gtagaagata tcattgatac aggtcaaact ttgaagaatt tgcgagatat
     6061 gtttaaagaa agagaagcag cttctgttaa aattgcaacc ttgttggata aaccagaagg
     6121 acgtgttgta gaaattgagg cagactatac ctgctttact atcccaaatg agtttgtagt
     6181 aggttatggt ttagactaca aagaaaatta tcgtaatctt ccttatattg gagtattgaa
     6241 agaggaagtg tattcaaatt agaaagaata atctttaatg aaaaaacaaa ataatggttt
     6301 aattaaaaat ccttttctat ggttattatt tatctttttc cttgtgacag gattccagta
     6361 tttctattct gggaataact caggaggaag tcagcaaatc aactatactg agttggtaca
     6421 agaaattacc gatggtaatg taaaagaatt aacttaccaa ccaaatggta gtgttatcga
     6481 agtttctggt gtctataaaa atcctaaaac aagtaaagaa ggaacaggta ttcagttttt
     6541 cacgccatct gttactaagg tagagaaatt taccagcact attcttcctg cagatactac
     6601 cgtatcagaa ttgcaaaaac ttgctactga ccataaagca gaagtaactg ttaagcatga
     6661 aagttcaagt ggtatatgga ttaatctact cgtatccatt gtgccatttg gaattctatt
     6721 cttcttccta ttctctatga tgggaaatat gggaggaggc aatggccgta atccaatgag
     6781 ttttggacgt agtaaggcta aagcagcaaa taaagaagat attaaagtaa gattttcaga
     6841 tgttgctgga gctgaagaag aaaaacaaga actagttgaa gttgttgagt tcttaaaaga
     6901 tccaaaacga ttcacaaaac ttggagcccg tattccagca ggtgttcttt tggagggacc
     6961 tccggggaca ggtaaaactt tgcttgctaa ggcagtcgct ggagaagcag gtgttccatt
     7021 ctttagtatc tcaggttctg actttgtaga aatgtttgtc ggagttggag ctagtcgtgt
     7081 tcgctctctt tttgaggatg ccaaaaaagc agcaccagct atcatcttta tcgatgaaat
     7141 tgatgctgtt ggacgtcaac gtggagtcgg tctcggcgga ggtaatgacg aacgtgaaca
     7201 aaccttgaac caacttttga ttgagatgga tggttttgag ggaaatgaag ggattatcgt
     7261 catcgctgcg acaaaccgtt cagatgtact tgatcctgcc cttttgcgtc caggacgttt
     7321 tgatagaaaa gtattggttg gccgtcctga tgttaaaggt cgtgaagcaa tcttgaaagt
     7381 tcacgctaag aacaagcctt tagcagaaga tgttgatttg aaattagtgg ctcaacaaac
     7441 tccaggcttt gttggtgctg atttagagaa tgtcttgaat gaagcagctt tagttgctgc
     7501 tcgtcgcaat aaatcgataa ttgatgcttc agatattgat gaagcagaag atagagttat
     7561 tgctggacct tctaagaaag ataagacagt ttcacaaaaa gaacgagaat tggttgctta
     7621 ccatgaggca ggacatacca ttgttggtct agtcttgtcg aatgctcgcg ttgtccataa
     7681 ggttacaatt gtaccacgcg gccgtgcagg cggatacatg attgcacttc ctaaagagga
     7741 tcaaatgctt ctatctaaag aagatatgaa agagcaattg gctggcttaa tgggtggacg
     7801 tgtagctgaa gaaattatct ttaatgtcca aactacagga gcttcaaacg actttgaaca
     7861 agcgacacaa atggcacgtg caatggttac agagtacggt atgagtgaaa aacttggccc
     7921 agtacaatat gaaggaaacc atgctatgct tggtgcacag agtcctcaaa aatcaatttc
     7981 agaacaaaca gcttatgaaa ttgatgaaga ggttcgttca ttattaaatg aggcacgaaa
     8041 taaagctgct gaaattattc agtcaaatcg tgaaactcac aagttaattg cagaagcatt
     8101 attgaaatac gaaacattgg atagtacaca aattaaagct ctttacgaaa caggaaagat
     8161 gcctgaagca gtagaagagg aatctcatgc actatcctat gatgaagtaa agtcaaaaat
     8221 gaatgacgaa aaataaccct gagagaggct ggagcctctc ttttttgtgc agtttaggag
     8281 ctaaagggaa cagaatggag aaaatggaac aaatgtgttt tctaatctgt tagactgtat
     8341 ctagaaaggg gaaaattatg attaaagaat tgtatgaaga agtccaaggg actgtgtata
     8401 agtgtagaaa tgaatattac cttcatttat gggaattgtc ggattgggag caagaaggca
     8461 tgctctgctt acatgaattg attagtagag aagaaggact ggtagacgat attccacgtt
     8521 taaggaaata tttcaagacc aagtttcgaa atcgaatttt agactatatc cgtaaacagg
     8581 aaagtcagaa gcgtagatac gataaagaac cctatgaaga agtgggtgag atcagtcatc
     8641 gtataagtga ggggggtctc tggctagatg attattatct ctttcatgaa acactaagag
     8701 attatagaaa caaacaaagt aaagagaaac aagaagaact agaacgcgtc ttaagcaatg
     8761 aacgatttcg agggcgtcaa agagtattaa gagacttacg cattgtgttt aaggagttta
     8821 ctatccgtac ccattagtaa gtcatgcaaa aaaaatgaaa aaaattagaa aaagtagttg
     8881 acaaagtttg aaaagactgt ataatagtaa gagttgaaaa taacaactca ggtccgttgg
     8941 tcaaggggtt aagacaccgc cttttcacgg cggtaacacg ggttcgaatc ccgtacggac
     9001 tatggtatgt tgcgtcagga ccacttgatg aaaaaaagtt taaaaaagct taaaaatctt
     9061 caaaaaagtg ttgacaagtg aaagtggctg tgatatacta atatagttgt cgcttgagag
     9121 aagcaagtga caaagacctt tgaaaactga acaagacgaa ccaatgtgca gggcgctaca
     9181 acataagttg tagtactgaa caatgaaaaa acaataaat
//
LOCUS       DAVLSL010000077         9214 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLSL010000077 DAVLSL010000000
VERSION     DAVLSL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.078"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            212..1021
                     /locus_tag="VXN30_001385"
     CDS             212..1021
                     /locus_tag="VXN30_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1357997.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(1202..2077)
                     /gene="aguB"
                     /locus_tag="VXN30_001386"
     CDS             complement(1202..2077)
                     /gene="aguB"
                     /locus_tag="VXN30_001386"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1357998.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(2087..3172)
                     /gene="aguA"
                     /locus_tag="VXN30_001387"
     CDS             complement(2087..3172)
                     /gene="aguA"
                     /locus_tag="VXN30_001387"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1357999.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPVVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(3169..4296)
                     /gene="nspC"
                     /locus_tag="VXN30_001388"
     CDS             complement(3169..4296)
                     /gene="nspC"
                     /locus_tag="VXN30_001388"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1358000.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(4296..5555)
                     /locus_tag="VXN30_001389"
     CDS             complement(4296..5555)
                     /locus_tag="VXN30_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1358001.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(5552..6412)
                     /gene="speE"
                     /locus_tag="VXN30_001390"
     CDS             complement(5552..6412)
                     /gene="speE"
                     /locus_tag="VXN30_001390"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1358002.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            6668..7383
                     /locus_tag="VXN30_001391"
                     /pseudo
     CDS             6668..7383
                     /locus_tag="VXN30_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(7349..8824)
                     /locus_tag="VXN30_001392"
     CDS             complement(7349..8824)
                     /locus_tag="VXN30_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1358003.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         2674 a         2072 c         1773 g         2695 t
ORIGIN      
        1 caaaaaaact attatattaa gttggttttt taatttgtct agttgcaaca ttgacaaaca
       61 tagtatagca tatctttgcc aaatatcctc ttcaaatcat gaattgtcat caaaacatct
      121 taaactataa aatcaattag tctcaagctt tctatcaatt tcttctccaa aatatgctat
      181 aatactacca aaagataaag aaggaagacc tatgattaaa ctactagcct tggatatgga
      241 tggaaccctc ctcaatgaag ccaaggaaat cccacaagct cacattactg ctattcacaa
      301 agccattgaa aaaggtgtca aactggttct ctgtacgggt cgcccccttt tcggtgtcct
      361 cccctactac aaaaaactgg ggcttgacct ccagaacgaa tatgttattg ttaacaacgg
      421 ttgttcaact caccaaacca gtgattgggg tctagttgac tggaaagaac tgagtccagc
      481 tgacatcgaa tacctctatg accttgctga aaagagtgat gttcagttga cactttttga
      541 cgagtcacat tattttgttc tcggtggcaa gcccaatcaa gttattgaaa atgatgctaa
      601 actagtattt tcagacctga ctgaaatttc tcttgaggaa gcgactagtg gaaagttccg
      661 gatgttccaa ggtatgtttt taggaacaaa agaacaaaca gacgattttg agcagcgttt
      721 tgctgaagag ctttgccaac gattcagtgg agttcgttcg cagcctgtca tttatgaagc
      781 aatgccactt ggaacgacaa aggctactgc tctttcacga ctagctgaga ttttgaagat
      841 tgattcctca gagattatgg ccatgggcga tgctaataac gatatcgaaa tgctccagtt
      901 tgcagggctt gggattgcaa tgggaaatgc cagcgattat gtcaaatctc ttgcggatgc
      961 cgttacctca agcaacgaag aagacggcgt tgcgcgtgct attgagaaat atattctata
     1021 aaaaagaaaa gcaaatagac agaagttact gctatttgct tttttgctaa tattttaaaa
     1081 tacggactaa tcaatagaca agaatagcga atcataaaaa tcgatttact agatgccata
     1141 gtatcttata gttgctaata agaagttagt ctagcagaat gaatctctca tttctcccac
     1201 actaatctgt aatttgtcta tacatttctg gtcttctatc tcgaaacaag ccccagttta
     1261 ggcgttcact tgctcccttg tctaggtcat aagtagctaa cagaacagct tcttcttgtc
     1321 tttcagctcg ttctagaata gctcctgttt catccgtcat aaaggaggaa ccgtagaagt
     1381 caagactgga actctgtccg ccattttcct cactaggagt aacctcctct aaaccataac
     1441 gattggctgc gatgactgga acaatattcg ctgctgcgtg cccttgcata gtacgttgcc
     1501 agtgaccaca actatctgta tccaaaattg gctctgaacc gatagctgta ggataaaaga
     1561 gcaattcagc accattcaat gcaagacagc gcgctgtttc agggaaccat tgatcccaac
     1621 agataccgat accaatctta gcatagcgag tattccagac cttgaaacca gtgttaccag
     1681 gcgtgaaata gaatttttct tgataataat ggtcatctgg tatgtgggtc tttcgataaa
     1741 cgcccagcac ttccccatct gcatcaatga cggcaataga gttatacaag acattaccat
     1801 ctttttcata gaaactgatt ggtaaaacaa cttgtagttc cttagcaatc accttaaaat
     1861 gctgaatggc agtattttcc gctacagatt gggcatactg gtagtagtca tactgacgtt
     1921 cctgacagaa atagggatgt tcaaacaact cgggcaagag aataatttgg gctccttgct
     1981 cagcagcctg acgtactaaa cgctctgcgg tttggatatt tgttgccaca tccttagcgc
     2041 attgcatctg aatggttgca actcttacat ttctcatctt tttctcctat tctggaattt
     2101 gttgggtgat acagtggata ttaccaccac ctaagagaat atctctggct ggtattccga
     2161 caactttacg gtctgggaaa cacttgctga ggatatctat ggccacttgg tcgtttacat
     2221 cctcaaactg tggaaccaag acagccttgt tggcgatata aaagtttacg taggaagctg
     2281 ctagtcgttc acctgcgtat cgctcttctt ctccttcttc atagatgtag cctggcaaat
     2341 cttcttctgt cacaacttgt cgaactacag ggataggcaa tttatgaatg gtgaagtgac
     2401 aaccttttgc atctgtttcc tgttctaaga gttcgagatc tgcttttgac atggcatact
     2461 ggggatcatt ttcgtcatct gtccaagcca aaacaagctc agcaggacca acaaaggcag
     2521 caacattatc gacgtgttca ttggtttcat cctgataaat accataagga agccaaataa
     2581 ctttttcagc accaagactt tctaataatg tgttttcaat ctcctcttta gtcaagttag
     2641 gattgcgacc aggactaagc aagcaacttt cagttacgag aatagttcct tgaccatcgc
     2701 tatggattgc gcctccttcc agtacaaaag gtttagcatc atagacaggc ctttccaaag
     2761 cctcagcaaa acgactggct acttggtcat cctcttcata atcttgataa agaccatcat
     2821 aggtgcctcc ccaagcattg aaggcccaat ccacggctaa tttcttacct ttatcattga
     2881 cgagaatggt tgggccagta tcacgcgccc aggcatcatt ggtgggaatg tctaaataaa
     2941 caaccttgtc tccaagatag gattgggctt cagatagata ggcctgctcc accaaaagat
     3001 agactctttc cccttctgct atggtctcga tgatctgagt aaatgctctt ttagcagcct
     3061 ttccttgaaa aggccatgat cctggtcgag tcggccatat catgagggta ccatgatggg
     3121 gttcgtactc tgctggcatg tgatagccta atttttttgg actgtccatc atgataatct
     3181 ccctttaaag tcttgatagc caaaagcttt gagtaagcta cagtctccct gttcgtccat
     3241 gagatagaga cttggcaatc caataccatt aaaggtatta tttttgacaa aagaataaat
     3301 ggccatgtct tgaaaataaa gtctgtctcc gatttggact ggattttcaa aactataatc
     3361 accaatcaca tcgccagtca gacaggtatt agaagaaagt ctgtaggtat gggctttttc
     3421 ctgtgcctca aagccatttc tcaaaggtgg acgatagggc atctcaagta catcaggcat
     3481 atggcaggtc gcagaggcgt ctaaaaccaa gatttccata ccgttttcta caatatctaa
     3541 tacctcagtt gctaaataac ccgcattaag cgcaatggct tcaccaggct cgatatagat
     3601 ttcaagattg taagtttttc ggatacgctt gatttctgaa atcagcaaat ccacatcgta
     3661 accttctctt gtaatatgat gaccaccacc catattgagc cattttacct catgtaagta
     3721 gggaccaaat tgttcttcta ctgctttcaa agttgtttgt aaatcatctg ctccctgctc
     3781 gcaaagggta tgaaaatgaa gtccgtcaac caaatctagc aaatcactcg gaatcttgtc
     3841 tatagtaact ccaaagcgag aacctggtgc acaagggtca tagagcgcgt gatctccttg
     3901 agttgaacac tgagggttga ggcgcaaacc aacactgaca ccagcctctc gacaacgagc
     3961 cccatgctta cgcaactgtc tctctgagtt aaagacgata tggtctgtta tcccaagcaa
     4021 ttcctccaag tctgcatcct tgaaagcagg cgcaaataca tggacttcac caggaaattc
     4081 ttcccttgcc aatttggcct catagagtcc actagctgtc gtacctgata gatactggct
     4141 aatcaaggga taagttttgt agagggaata tgccttctgg gcaagcaaga ccttgcaacc
     4201 ggcctcttct tgtacatatt gtagaatgcg gcaattagct tctaacttgg ccaagtcaat
     4261 aacataggct ggtgttggta cttgttctaa cttcattagt ccaccatttg tggattttca
     4321 accacaaccc atggcaaacc atactcattc aaagcttcca tgaatggatc tggatctaac
     4381 tcctcaaggt tatacactcc agcttgtttc caagttccgt tcatgactaa ttttgtccca
     4441 atcatggctg gaactcctgt cgtgtatgaa attgcttgtg acccgacttc tgcatagcac
     4501 tcttgatggt cgcaaacatt gtagatgtag atagtctttt caacgccatc tttgacacct
     4561 gtaaagatac atccaatatt ggttttacct acagtacgtg ggccaaggct agcaggatct
     4621 ggaagcaagg ctttcaaaaa ttgaattgga acaatttctt ggccgttaaa gttaatggta
     4681 tccgtacgaa ggagtccaac attttcaaga catttcatgt gcgtcaagta agattgacca
     4741 aaagtcataa agaaacgaat gcgtttgaca cctggaatgt tcttggccaa tgattcgatt
     4801 tcttcatggt gaaggagata catatctttt tgtccaactt gagggaaatc atactcacgc
     4861 ttgatagaca tagcttcgac ttcgacccat ttcccatctt cccagtaaga acctggcgca
     4921 gaaacctcac ggagattaat ttctggatta aagttggttg caaatggata accgtggtca
     4981 ccgccattac agtctaaaat gtcgatataa tggatttcat caaaatagtg tttgagggca
     5041 taagctgaaa agacactagt tacacctggg tcaaaaccag aaccaagaag agcagtcaag
     5101 cctgcttctt tgaatttctc ttgataagcc cactgccatg agtagtcaaa gtaggctgta
     5161 aaaccaagtt ccttacaacg tttttcgtag atagcacgcc actcagggtc ttctgtgtct
     5221 tctgcttcgt agttggctgt atcgatatag tgaacacctg ttgccaaaca agcatccata
     5281 atggttaaat cttgataagg cagagctaca ttcaaaacag cttctggttt gtagctttca
     5341 atcagggcaa tcacttcttc aaccttgtca gcatcaagtg ctgcagtttc aattttagta
     5401 cttgttttgc cttctagctt cgctttcaag tcatcgcatt ttgacttggt acggctagca
     5461 atcataatct ctgtaaatgt ttcgctatct tgacaaatct ttgaaatagc aacttgggca
     5521 acgcccccac aaccaataac tagtaaacga ctcatttttt tccttcctct tcttctaaaa
     5581 tgtcctcaac atacttgggc aacataaagg atcccacgtg taagtttgca gtgtagtatt
     5641 ctgtgaaaag ctggcgtttt ttccaacctt ccttgtcaaa atctttgaca gggtggtatt
     5701 ttttcgatgc aaatccaaac aaccaatagc cagctgggct agttggaata tgggcctgat
     5761 aaacccgact gattggaaag gcttgattga ccttgcggtg catgcttcgg caggccgact
     5821 catcctcgtc aaagaaggga ctcccatgct ggtaaatcat gatgccgtct tccttcagag
     5881 ctcgataact attgccgtag aattccttgg taaagagtcc ttccgtatgg ccaaatggat
     5941 ctgtcgcatc gttgatgata atatcgtaat catcttcgca gtttcgcaaa aagcgtagcc
     6001 cattttggta gtaaatggta acacgaggat catctagccc tgcagcaaag tctgggaaat
     6061 actcacgaca gacctcgacc aacatctcat ccggttccac gatatcgatt tgttccagtt
     6121 caggatagag tgttaatact tgggcaacac cgccgtcacc acccccaata accaatactt
     6181 tctttggatt tgggtggaca gccatgggaa cgtgaacggt catttcattg tagacgaaat
     6241 catccgcatc tgagaacaag acatggccat ttaaaatcag tattttccca aaagctggcg
     6301 tatccaagac ttcgatatcc tgccattcac tttttccagc gtaaagttgc ttggctgttc
     6361 tcagagacaa tttgacatct ggagtatgaa cttcagaaaa ccataaatcc atctatttct
     6421 ccttatatta tatttagcgc agttagctac tgccaaagcc caagtggtat acttggaata
     6481 agccactgtg gattagttta ttccctccaa tcgcttgacg atttccgaaa ggctctaacc
     6541 acagtttttt gtttcaattg gtaatgagtt tgataacgca tagacgtttt atctcgtcta
     6601 aggtaacaag gcaaaaaatt cgtggataac caagtagact tgaaatacta catctggagg
     6661 tactcccatg ctttatgtgg gcattgatgt cgctaaaaat aaacacgatg ttacagcctt
     6721 gaatgttcca ggaaaaactg ttcttaaacc actcactttt tcaaataata aagctggttt
     6781 tgaactctta gatctgtctc ttcgacaact caaccaagac tgtctcatcg ctcttgaaaa
     6841 taccggacat tatgccttta atctactgaa cttcctgcat gaacaaggat ataaagttta
     6901 cacctacaat cctttgctca tcaaggaatt tgccaaatca ttatcacttc acaagactaa
     6961 aacggataag aaagacgctc atggcatcgc tcttaaactt ctttctgacc ccaatcgtga
     7021 acaatttcaa cacgataatc ggcaagtaga actaaaaata ctggctagac atattcatcg
     7081 tctcaagaaa aacagtctga ttggaaagta caatacactc gttgtcttga tatcatcttt
     7141 cctgagttgg ataaaatcgt tggaaagcat tcagaatata cctaccaact cttgacacgc
     7201 taccctaatc ctcagaaaag gattgaggca ggatttgata agctgataga aattaagcga
     7261 ttgaccgctt ctaattcaag ggctttttgc cgtgctctaa aatcatcgcc aaatcatcaa
     7321 attgaaataa gaaatagttt agataaaact attgactttt cttatagttt gtctttctct
     7381 taataacgtt gatatgattg acctctggat cttccgtccc ttggagggag caaccacgtt
     7441 ccttggcgaa ttggatatag tctacaattt ctcgtgtaat gcgttcacca ggagccaaga
     7501 taggaatccc tggaggatag cacatgacaa attctccaca gacctgtcca acagactcat
     7561 ctaaggtcaa actttttctc tctgaataga aggcttcttg tggagacagc accaactcgg
     7621 gctgaatata ttctcctgct atcaaatctt ttccatctct tgaatagagt ctcttaatat
     7681 cagccagagc accaaccaag cgctcgatgt cttggatgcg gtcgccgatg gaaatatagg
     7741 ccaagatatt gccgatatca ccaaactcga tctgaatgtc gtattcgtct cgcaagaggt
     7801 cataaacctc gatacctgtt aagccaatac cctgagtgta aactgacagc ttagttacgt
     7861 caaaatcgca aaccgaaaca ccgtctatta actcttttga gtaggcatag tagccaccga
     7921 tagcattgat ttcacggcgg gcatactcag atagctcaat gactttctca aacgactctt
     7981 taccacgaag ggccaagttg cgacgtgaaa tatccaaact agccatcaac aagtaagagg
     8041 cagatgtaga ctgagtcagg ttaattatct gacgaacgta ttcagaattc atctgctccc
     8101 cgattaaaag aagcgaactt tgtgtcaaac tcccaccaga cttatgcatg gaaactgctg
     8161 ccatatcagc tcctgcatcc atagcagaaa ttggaagttt accagtgaaa tgcaaatgcg
     8221 ctccatgggc ttcatctact aaaaccatca tgccagcttc atgagccatt tctgtcaacc
     8281 cctttaggtc tgaacagatg ccgtagtaag taggattgtt gattaggata gccttagcat
     8341 ctggatggtc ctttatggcc tgtgctactc ggtcattttc aagacctaaa gcgataccaa
     8401 tcttaggatc tacactcatc tcgatataga tgggaatggc accacataga accaacgcat
     8461 tgatagcaga tttatggaca tttcgtggca gaataatctt atctcctgcc ttgcaggttg
     8521 ccagaatcat agtctgcacc gatgaagttg ttccaccaat cattagaaag gcatggctag
     8581 ctccaaaagc atctgcagcc agctcctctg catcacgaat aatcgaaata ggatggccta
     8641 aattatccaa gggtttcatc gaattgacat caatgcctac acatttttct cctaagagtt
     8701 cgacaagttc tggatttccc cgtccacgct tgtgacctgg aacatcaaag ggaacaatcc
     8761 ttttcttgcg taacttcacc aaggcctcat aaattggggc ttggttttga tctaactctt
     8821 tcaaaacata ctcctttcaa tatttttagg accttatgaa aactaaggaa ctaaaggttg
     8881 actcatgaaa aaagagcagg ttttcacctg ctctgcaatc ttctgacgtg tttattttgg
     8941 tttctgttga gtatgtctgt tcccgatgtt tcctaactct ctagtagcaa atattacgta
     9001 ctttattgat cgtttcacaa gatgacgtgt gctttcacca cactaaaaat ttatgaatta
     9061 ggacaagtgt cctaacatca acttataaaa gtaggctttt aaccctatca ataatgtggc
     9121 ttttcaacca cgcttcggca ttcaaccctg cctgtccgta ggcatttttt actcgggttt
     9181 atatttgcta gaaaacatca tctcattttc taag
//
LOCUS       DAVLSL010000078         9158 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLSL010000078 DAVLSL010000000
VERSION     DAVLSL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.079"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            150..470
                     /locus_tag="VXN30_001393"
     CDS             150..470
                     /locus_tag="VXN30_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1358004.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            474..1289
                     /locus_tag="VXN30_001394"
     CDS             474..1289
                     /locus_tag="VXN30_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358005.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSIFTISLVIGIWNWKEAVFRPFIS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            1364..2290
                     /locus_tag="VXN30_001395"
     CDS             1364..2290
                     /locus_tag="VXN30_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1358006.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDVLMESDQVAEAFNLLYKQGKVRDFGVSNQNPIMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            2356..2622
                     /locus_tag="VXN30_001396"
     CDS             2356..2622
                     /locus_tag="VXN30_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1358007.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            2615..3313
                     /locus_tag="VXN30_001397"
     CDS             2615..3313
                     /locus_tag="VXN30_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1358008.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKSLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            3324..3803
                     /locus_tag="VXN30_001398"
     CDS             3324..3803
                     /locus_tag="VXN30_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1358009.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQYRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            3811..4539
                     /locus_tag="VXN30_001399"
     CDS             3811..4539
                     /locus_tag="VXN30_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358010.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEVFELEEILI"
     gene            4581..5450
                     /locus_tag="VXN30_001400"
     CDS             4581..5450
                     /locus_tag="VXN30_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1358011.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            5425..5865
                     /locus_tag="VXN30_001401"
     CDS             5425..5865
                     /locus_tag="VXN30_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1358012.1"
                     /translation="MIPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLGDLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            5988..8534
                     /locus_tag="VXN30_001402"
     CDS             5988..8534
                     /locus_tag="VXN30_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1358013.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(8592..>9158)
                     /locus_tag="VXN30_001403"
     CDS             complement(8592..>9158)
                     /locus_tag="VXN30_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV1358014.1"
                     /translation="LDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQ
                     DSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFF
                     GLIEDNLKQVHPIFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         2753 a         1543 c         2078 g         2784 t
ORIGIN      
        1 aaccatacat acggtagggc gacgctgacg tggtttgaag agattttcga agagtattaa
       61 ctattatgct ttacaaacta gtgtagaagt aatatactat aggtgaagtt actagtaggt
      121 attacaaaat agattagaag ggagaagtga tgaaagaaac tcagctatta aaaggtgttc
      181 ttgaaggttg tgtcttggat atgattggtc aaaaagagcg gtatggttat gagttggttc
      241 agactttgcg agaggctgga tttgatacta tcgttccagg aactatttat cctttgttgc
      301 aaaagttaga aaaaaatcaa tggataagag gcgacatgcg cccgtcgcca gatggtccag
      361 atcggaagta tttttcatta atgaaagaag gagaagagcg tgtctcagtc ttttggcaac
      421 aatgggacga tttgagtcaa aaagtagaag ggattaagaa tgggggttaa accatgaaga
      481 aaatgaagta ttacgaagaa acaagcgctt tgctacatga gttttctgag gagaatcaaa
      541 agtattttga ggagttgtgg gaaagtttta atcttgctgg atttctctat gatgaagact
      601 atctcagaga gcagatctat ttgatgatgc tagatttctc agaagcagaa cgagatggca
      661 tgagtgcaga ggattatcta ggtaagaatc ctaaaaaaat aatgaaagag attctcaagg
      721 gagcacctcg cagttctatc aaagagtccc ttttgacgcc aattcttgtc ctggcggtat
      781 tacgttatta tcaactacta agtgattttt ctaaaggtcc tctcttaaca gtcaatttgc
      841 tcacattttt agggcaactt cttatttttc tgattggatt tggacttgtg gccacaattt
      901 tacgaagaag tttagtccaa gattctccta aaatgaaaat tggcacttac attgttgttg
      961 ggactatagt tcttctagtt gttttaggat atgtaggaat ggcaagcttc atacaagaag
     1021 gagcctttta tattccggct ccctgggata gtttgtctat ctttacgatt tcgctagtta
     1081 tcggtatttg gaattggaaa gaagcggtct ttcgtccatt tatcagtatg attattgccc
     1141 atcttgtggt gggttctctg ctccgttatt atgagtggat gggaatttca aatgttttcc
     1201 ttacaaaagt tattccttta gctgtcctct ttattggaat ctttgtcttg ttccgtgggt
     1261 ttaagaagat aaaatggagt gaagtatagt caaaaagtcg ttgcaaagcg gttttttgtt
     1321 ataatgaagg gaagaatgtt agaaattaaa ggagaaaaat ttgatgagat acataactct
     1381 tggtcaagat gacaaagaat tatcagaaat tgttctcgga atgatgagaa taaaagataa
     1441 atctgtaaaa gaagttgaag agcttgtaga aacagcactt tctgttggaa tcaatgcctt
     1501 cgacttggct gatatttatg gtcgtggtcg ttgtgaagaa ctgttaggtc ttgtcctaaa
     1561 aaatcgtcca gatttaagag aaaagatgtg gattcagtcc aaatgtggca ttcgcattga
     1621 agaatttacc tattttgatt tttctaagga ctatattata aaatcagtag acggtatttt
     1681 gcaaagattg aagattgatc atctagatag cttgctcctt catcgaccag atgttttgat
     1741 ggaatctgac caagtagcag aagcctttaa tctcctttat aaacaaggta aagttcgaga
     1801 ttttggagtt tctaatcaaa atcctataat gatggagttg cttaaaaaag atgtcaagca
     1861 gccgttagct gttaatcagc tacaattgag tgcggctttt actccaggat ttgaatcagc
     1921 ttttcatgtt aatatggaag atagtcaagc agctatgcga gatggcagca tttttgaata
     1981 ttgccaatta cacgatgtgg tcattcaagc atggtctgtc ttacaattcg ggtattttaa
     2041 agggaatttt gttggaaatg agaaatttca agctttaaat caagtacttg atcgtttagc
     2101 tattaaatat ggagtaacct cttcaactat tgccatttct tggatattgc gttatccagc
     2161 aaaaatgcag gcagtcgtag gtacaacaaa tcctaagcac ttgagagaag ttagccgagc
     2221 ggcaaacttt agcttaacaa gaaaagaatg gtatgaaatt tatcttgctg cagggaataa
     2281 tttgccgtaa gtagtagcag gtttgaataa ctcacattca aaaagccgta taaagcggtt
     2341 ttttgctata attaaatgaa gaatgtagaa ttaaaggaga aaaacatgac atttgaagag
     2401 atcctgcctg ggttaaaggc taagagaaaa tatgtacgaa ctggttgggg aggtgcggaa
     2461 aactatgtcc aactctttga taccatcgag caaaatgggc ttgcacttga aatgacacct
     2521 tatttcctaa tcaacgtttc tggcgaagga gaaggttttt ccatgtggag cccgacagtt
     2581 tgtgatgttt tggcaacgga ttgggtagaa gtgcatgact aaacgtgtac tcattacagg
     2641 agtgagttca gggatcggat tggctcaagc tcgcctcttt ttagagaagg gctatcaagt
     2701 ttatggagtt gaccaaggtg aaaagtcact cttagagggt gattttcact ttttacagag
     2761 agatttgacc ttggacttgg aacctatttt tgactggtgc cctcaggtgg atgttttgtg
     2821 taatactgct ggagttttgg atgattacaa accactgttg gaacaaacgg cgcaggacat
     2881 tcaagagatt tttgaaatca actacattat tccagtagag ttgactcggt attatttgac
     2941 acaaatgctg gaaaataaaa agggaatcat catcaatatg tgttccattg cttctagcct
     3001 agcaggtgga ggtggtcacg cctatacttc atccaagcat gccttggctg gcttcaccaa
     3061 gcagttggct ctagactatg ctgaagctgg gattcaggtc tttggtatcg ctccaggagc
     3121 agtcaagaca gctatgaccg ctgcggattt tgaaccaggt ggtttggctg actgggtggc
     3181 tagtgaaacc ccaatcaagc gctggattga gccagaggaa atagcagagc ttagtctttt
     3241 tttagcaagt ggaaaagcga gcgccatgca aggacaaatc ttgacaatag atggtggctg
     3301 gtctttgaag taggaggagt tggatggaaa tcaaaaaaca ctttggagtc tacgctgttt
     3361 gcttagaaaa tgggaagtta ctctgcattg aaaaaacgag aggcccttat caatatcggt
     3421 atgatctacc tggaggcagt cagcaacttg gtgaaggact gacggaaacg ctgactagag
     3481 aagttatgga agagacggga tttactgtta gaagctactc taatcctcga atctacgatg
     3541 ttttcgttag ggaagagtta aaaaatttta tggttcacca tgtcatggcc ttgtatgatg
     3601 ttgaaatgaa tgagagtgca cctcaagtta cgacttcgga agctgtgtct gatggtgcga
     3661 atgattcact tggatatatt tggatggata ttcaagagat tacagaagaa aatgcatcgc
     3721 cattagtctt gaaggttaag tctgaattat taggatttcc agaactggac aagacttctt
     3781 acatgaattg gaaggtgaag tagagggata atggaactat ttaaaacatg gaagaaaaat
     3841 atggttctct atggtcttaa atctcaaatt ggaactgtct accgaaacaa tgataggaca
     3901 acaagctttt atgatgttgg gaattttcta tacctagcag gggagttgga ttccagattt
     3961 tgggaagatt ttgttagaaa atatggttta gattataaga ttattatttc agaaaatact
     4021 aattggcaag attttctgca tcgaaaagtg gggctaaatt cttttactcg ctattctttt
     4081 aaagataagg caaattttca agttgaattt ttaaataatc tagttactca tttagaggaa
     4141 ggttacaata ttgtgcctat tgataatcat atttataact gcttttctac ggaagaatgg
     4201 tcacaggatt tacaggggga ttttgagtcc tatcaggatt ttgttttaaa aggtggattt
     4261 ggctttgtga ttcttaaaaa taatgaactg attgctggga tttcctcagg gttagtttac
     4321 cgtaaagcag ttgaagtgga agttgcaact agaccaaacg aacaaggaaa tggatttgct
     4381 aaaaagcttg gtgctgcaat gattctagag agtttaaata gagatatgtt tccactttgg
     4441 gatgctcata atgaggcttc caaaaaagta gcagaatttt taggatatga gttatctgaa
     4501 ccttatgaag tttttgaact agaggaaatt ttgatataat tatatatgat attgtatctt
     4561 ttaagacgag gtgaatggct atgagaaatc aacttgccct tagtggcgaa aaaattcttg
     4621 aaaaaattta tcctcaacta tttcaccacg tcggcatgat tcgtggggaa tacttattga
     4681 gagagcttaa tcaaaacatc atattagcaa gttgtcagca atttgtaaaa gattatcttg
     4741 atactatttg ctccttttac ttagataagg aagtttggta tcgtttttct gaattaacga
     4801 atacagaagc caattgttta gcggggacta gagagttttt tgacgaaggg catcccttgt
     4861 ttggctatag aggaaccagg cgtttactgg cgtgtttgga tgaatttcag gctgaagcac
     4921 atgtcgttac agaagtttat caaaccaatc ccaatctgtc tcttattttt ccttttgtca
     4981 atgatgccga ccaattaaaa caagctatta cagtattgcg tcagtatggc tttactggga
     5041 aagtcggaac gatgattgaa ttaccgtcag cgtattttga cttatctagt atactggaaa
     5101 cgggcatttc aaagattgta gttggaatga atgatttgac ttcctttgtt tttgcgacta
     5161 tgagaaacag tcaatggcat gatatggaaa gtccaataat gttagatatg ctaagagata
     5221 tgcaggataa agcaagaaag aacaagatta attttgctgt agcaggctat ctgaatactt
     5281 ctttcataca aaaaatgaat cagttgggta tcaagtgcat tatccactac agttctattc
     5341 cagagatttt tgatttagag attgaccatc cagatcatct taaacacata aaagaagaaa
     5401 gtaaaaaatt acaaaggagt acccatgata ccgcaagaaa tgtggaataa atacaagcaa
     5461 atcaatcctt ctatcggaga tgaaatcgat gcttgggctt ttggagttga accagacctt
     5521 ttaggggatt tggtgtttaa aggagaaaag acagcaacag catcagccta tgacctctat
     5581 gtactagaag acgaaccact tccccaagta gggaccttcg atgtcatttt agatagtcaa
     5641 aatcaggctg tctgcattgt cgaaattaca aaggtttctg ttgaactctt caatcaagtt
     5701 tctgcgcaac atgcctttaa ggaaggtgag ggagacaaat cacttgccta ttggcgccag
     5761 gttcatgagg actttttcac agactgtttg ggtgaagtag ggctgacttt tacacctgaa
     5821 agcaaggttg ttttagaaga atttcgcaag gtctacccac tgtagactat tagaaggaag
     5881 aaagttttgg aaatcgctgt ccaatccttt tttctcaagc aaaatatgat ataataagtt
     5941 tgtttgaaga agagcagcag ctcttaaact tagaatagga gaaaactatg caagcagttg
     6001 aacattttat taagcaattt gttcctgaac attatgattt atttttagat ttgagtcgtg
     6061 agaccaagac tttttctggg aaagtgacca tcactggtca agcacagagt gaccgcatct
     6121 ccctccacca aaaagacttg gaaatcacct ctgtagaagt tgcaggtcaa gctcgtccat
     6181 ttacagttga ccatgacaat gaagcccttc atatcgaatt ggctgaggct ggtcaagttg
     6241 aattggttct tgccttttct ggtaaaatta cagacaacat gacagggatt tacccttctt
     6301 attatacagt tgatggagtc aagaaggagg tcttgtctac tcagttcgag agccattttg
     6361 cgcgcgaagc tttcccatgt gtggatgagc ctgaagccaa agcaactttt gacctctctc
     6421 ttcgctttga ccaagcagaa ggtgaattgg ccttgtcaaa catgccagaa atcgatgttg
     6481 aaaaccgtaa ggaaacaggt atctggaagt ttgagacaac acctcgcatg tcttcttact
     6541 tgttggcctt tgttgctggt gatttgcaag gggtgaccgc taaaactaaa aatggtaccc
     6601 tggtaggtgt ctactcaacc aaagcacatc cactttcaaa tcttgatttc tcactggata
     6661 tcgctgttcg ctctatcgag ttttacgaag attactatgg agttaagtac ccaattcctc
     6721 aatctctcca catcgccctt cctgacttct cagctggtgc tatggaaaac tggggtcttg
     6781 tgacctaccg tgaagtttac ttggttgtcg atgagaactc tacatttgct agccgtcaac
     6841 aagttgccct tgttgtggcc catgaattgg ctcaccaatg gtttgggaac ctcgtgacta
     6901 tgaaatggtg ggatgacctt tggctcaatg aaagtttcgc taatatgatg gaatacgtct
     6961 gtgtggatac catcgaacca agctggaata tctttgaaga tttccaaaca ggtggagtac
     7021 ctcttgctct tgaacgtgac gctactgatg gcgttcagtc tgtccacgtc gaagttaaac
     7081 atccagatga aatcaataca ctctttgacg gcgctatcgt ctatgccaaa ggaagccgtc
     7141 tcatgcacat gcttcgccgt tggctaggag atgctgattt tgctaaaggt ttgcatgcct
     7201 actttgaaaa acaccaatac agcaacacca ttggtagtga cctttgggat gcccttggtc
     7261 aagcgtcagg acgtgatgtc gcagccttca tggattcttg gttggaacag cctggttacc
     7321 cagttcttac tgtcaaagtt gaaaatgatg tcttgaagat ttcacaaaaa caattcttca
     7381 tcggtgagaa cgaagacaag aaccgtctct gggtggtgcc cctcaatagc aactggaaag
     7441 gcttgcctga tacactcgaa actgaaagta tcgaaatccc tggctacgca gctcttcttg
     7501 ctgaaaatga aggagctctt cgtctcaaca ctgaaaatac tgcccactat attaccgact
     7561 atcaaggaga cttgttagaa gctgttcttg ctgagctaga gacacttgat aacacaagca
     7621 aactgcaaat tgttcaagaa cgtcgtttgt tggctgaggc agggcacatt tcttatgcag
     7681 acttgcttcc agttcttgat aaacttgcta aggaagagtc ttatctggtg gtttcagctg
     7741 tttctcaagt gatttcagcc cttgagcgct ttattgatga aggaacggat gctgaaacag
     7801 cctttaaagg actggttgct aaattggctc gtcataacta tgaccgtctt ggttttgaag
     7861 ctaaagacgg agaatcagat gaggatgaat tggttcgtca gctggctgtt tcgatgatga
     7921 ttcgctcaaa tgatgcagaa gctagtcaag tcgctagcca aatcttcgca acacacaagg
     7981 agaatcttgc aggactccca gcagctattc gctcacaagt tctgattaat gagatgaaac
     8041 atcatgagac taaagacttg ttagcacttt atcttgatac ttatactcac gcaacagatg
     8101 ctgtctttaa acgccagttg gcagcagctc ttgcatacag tacagatgcg gataatatcc
     8161 aaaacctgat tacttcttgg aaggacaaat ttgtggtcaa accacaggac ttgtctgctt
     8221 ggtattacca attcctagct catcaagcaa ctcagaaaac agcttggtct tgggcgcgtg
     8281 aaaactgggc ttggattaag gcagcccttg gaggagatat gagctttgat agctttgtta
     8341 tccttcctgc tcatgtattt aagactcagc aacgcttggc ggaatacaag gagttctttg
     8401 agccgcagct ttctgacctt gctcttagcc gtaacatcgg tatgggaatc aaggaaattg
     8461 cagcacgtgt tgacttgatt agccgtgaaa aagctgcagt cgaagcagtt gttcttcaat
     8521 acggaaatgc ataaataagc ctaaaataaa aagaaaaggc tctttgtcaa ctgtagtggg
     8581 ttgaaaaaaa gctaagctcg agaaaggaca aattttgtcc tttctttttt gatattcaga
     8641 gcgataaaaa tccgtttttt gaagttttca aagtttcgaa aaccaaaggc attgcgtttg
     8701 ataagtttga tgagattatt ggtcgcttcc aatttggcgt ttgaataggg tagttgaagg
     8761 gcgttgacga ttttctcttt gttctttaga aaggttttaa agacagtctg aaaaatagga
     8821 tgaacctgct tcagattgtc ctcaatgagt ccgaaaaatt tctcagggtc tttgttctga
     8881 aagtgaaaaa gtaagagttg atagatctga tagtggtgtt tcaagtcttc tgaatagctt
     8941 aaaatcttgt caagaatttc tttatttgtt aagtgcatgc gaaaagtagg gcgataaaaa
     9001 cgtttatcgc tgagtttacg actatcctgt tggatgagtt tccagtaacg cttgatagcc
     9061 ttgtattcat gagattttcg ttcaaactga ttcataattt gaacacgaaa acgactcatg
     9121 gcacggctga gatgttggat aatatggaaa cgatctag
//
LOCUS       DAVLSL010000079         9136 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLSL010000079 DAVLSL010000000
VERSION     DAVLSL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.080"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            77..760
                     /gene="rpiA"
                     /locus_tag="VXN30_001404"
     CDS             77..760
                     /gene="rpiA"
                     /locus_tag="VXN30_001404"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1358015.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            774..1985
                     /locus_tag="VXN30_001405"
     CDS             774..1985
                     /locus_tag="VXN30_001405"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1358016.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            1987..2520
                     /locus_tag="VXN30_001406"
                     /pseudo
     CDS             1987..2520
                     /locus_tag="VXN30_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285096.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            2544..3353
                     /locus_tag="VXN30_001407"
     CDS             2544..3353
                     /locus_tag="VXN30_001407"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1358017.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            3829..4224
                     /locus_tag="VXN30_001408"
     CDS             3829..4224
                     /locus_tag="VXN30_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV1358018.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            4234..4809
                     /locus_tag="VXN30_001409"
     CDS             4234..4809
                     /locus_tag="VXN30_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1358019.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            4834..5346
                     /locus_tag="VXN30_001410"
     CDS             4834..5346
                     /locus_tag="VXN30_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1358020.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            6004..6714
                     /gene="deoD"
                     /locus_tag="VXN30_001411"
     CDS             6004..6714
                     /gene="deoD"
                     /locus_tag="VXN30_001411"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1358021.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(6737..7261)
                     /locus_tag="VXN30_001412"
     CDS             complement(6737..7261)
                     /locus_tag="VXN30_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1358022.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(7314..7550)
                     /locus_tag="VXN30_001413"
     CDS             complement(7314..7550)
                     /locus_tag="VXN30_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1358023.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(7618..8538)
                     /locus_tag="VXN30_001414"
     CDS             complement(7618..8538)
                     /locus_tag="VXN30_001414"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1358024.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            8728..9132
                     /locus_tag="VXN30_001415"
     CDS             8728..9132
                     /locus_tag="VXN30_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358025.1"
                     /translation="MKILKRYILELCFILSFALPFTKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFICSILIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
BASE COUNT         2747 a         1616 c         2026 g         2747 t
ORIGIN      
        1 ttctaaaggt acttttagat gaaataaaag cctttacatg gtataataga ggtagctctt
       61 taatggaggt gtttgagtgg aaaatctgaa gaaaatggca ggtatcaagg ctgctgagtt
      121 tgtcaaggat ggaatggtcg ttggacttgg aacaggctcg actgcctatt attttgtcga
      181 agaaatcggt cgtcgtatca aggaagaagg attgcagatt atagctgtaa cgacttctag
      241 tgtgaccact aaacaggcag aaggccttaa tatcccactc aaatctattg accaagtaga
      301 ctttgtcgat gtgacagttg atggcgcaga tgaagtggat agtcagttta atggaatcaa
      361 aggtggtggt ggtgctcttc tgatggagaa ggttgttgcg acaccatcaa aagaatacat
      421 ttgggtggtg gatgaaagca agctggtcga aaaactaggt gcttttaaat tgccagtaga
      481 agtggttcag tatggtgcag aacaggtctt tcgtcgtttt gaacgagctg gctacaaacc
      541 aagtttccgt gaaaaagacg gccaacgttt tgtgacggat atgcagaatt ttatcattga
      601 cctcgccttg gatgtcattg aaaatccaat tgcttttgga caagaattgg accatgtcgt
      661 tggtgttgtg gagcatggtt tattcaacca aatggtggat aaggtcatcg ttgctggacg
      721 ggatggagtt cagatttcaa cttcaaaaaa aggaaaatag aagggggcat aagatgtcta
      781 aatttaatcg tattcatttg gtggtactgg attctgtagg aatcggtgca gcaccagatg
      841 ctaataactt tgtcaatgca ggggttccag atggagcttc tgacacactg ggacacattt
      901 caaaaacagt tggtttgaat gtcccaaaca tggctaaaat aggtcttgga aatattcctc
      961 gtgaaactcc tcttaagact gtagcagctg aaagcaatcc aactggatat gcaacaaaat
     1021 tagaggaagt atctcttggt aaggatacta tgactggaca ctgggaaatc atgggactca
     1081 acattactga gcctttcgat actttctgga acggattccc agaagaaatc ctgacaaaaa
     1141 tcgaagaatt ctcaggacgc aaggttattc gtgaagccaa caaaccttat tcaggaacgg
     1201 ctgttatcga tgattttgga ccacgtcaga tggaaactgg agagttgatt atctatactt
     1261 cagctgaccc tgttttgcag attgctgccc acgaagacat tattcctttg gatgaattgt
     1321 accgtatctg tgaatacgct cgttcgatta cccttgagcg tcctgccctt cttggtcgca
     1381 tcattgctcg cccttatgta ggtgaaccag gtaacttcac tcgtacggca aaccgtcgtg
     1441 acttggctgt atctccattt tccccaactg ttttggataa attgaatgag gctggtatcg
     1501 atacttatgc tgtgggtaaa atcaacgata tctttaacgg tgctggtatc aaccatgaca
     1561 tgggtcacaa caagtcaaat agtcatggaa ttgatacact attgaagact atgggacttg
     1621 ctgagtttga aaaaggattc tcattcacaa acctagttga ctttgatgcc ctttacggcc
     1681 atcgtcgtaa tgctcacggt taccgtgatt gcttgcatga gtttgatgaa cgcttacctg
     1741 aaattatcgc agctatgaga gagaatgacc ttctcttgat tactgcggac catggaaatg
     1801 acccaacgta tgcaggaacg gatcacactc gggaatatat tccattgttg gcctatagcc
     1861 ctgcctttaa aggaaatggt ctcattccag taggacattt tgcagatatt tcagcgactg
     1921 ttgccgataa ctttggtgtg gaaactgcta tgattgggga aagtttctta gataaattgg
     1981 tataagatga cgcgctatgc tttgctggtg agaggtatca atgttggtgg taagaataag
     2041 gtcgtcatgg cggagcttcg tcaagaattg acaaacttgg gactggaaaa ggttgagagc
     2101 tacatcaaca gtggcaatat tttctttact tcgatagatt ccaaagccca attggttgaa
     2161 aagctagaga ctttctttgc agtccattat ccatttattc agagcttttc tttactgagt
     2221 ctagaggact ttgaggcgga acttgaaaat ctaccagctt ggtggagcag agatttggca
     2281 cgaaaagatt ttctctttta cactgagggt ttggatgtgg accaagtcat cgcgacagtt
     2341 gaaagtttag agctgaaaga tgaagtgctt tattttggaa aacttgggat tttctagggg
     2401 aaattttctg aagaatccta ttctaagact gcctatcata agtacttgct gaggtgcctt
     2461 tctaccgcca cattactatt cgtaatgcta aaacctttga caaaattggt caaatgctaa
     2521 aaaaataata aaggagacac acaatgacat ttttaaacaa aatccatgaa actgctactt
     2581 tcctgaaaga aaagggaatt gcagcccctg agttcggtct aatccttggg tcaggacttg
     2641 gagaattggc agaagaaatc gaaaatccag ttgtagtaga ctatgctgag attccaaact
     2701 ggggccgttc aacagtagtc ggtcatgctg gtaaattggt atatggtgaa ctggcaggtc
     2761 gcaaggtctt ggctcttcaa gggcgtttcc atttctatga agggaatcct ctggaagtgg
     2821 tgactttccc agttcgtgtg atgaaagttc ttggatgtga aggtgttatt gtaaccaatg
     2881 cagctggcgg tatcggattt ggtcctggta ccttgatggc tatctcagac catatcaaca
     2941 tgacggggca aaatccattg atgggtgaaa acttggatga ctttggccca cgtttcccag
     3001 atatgtctag ggcctacaca ccagaatacc gtgctactgc ccatgaagtg gctaaaaaac
     3061 ttaatatcaa gcttgatgaa ggtgtctata tcggagttac tggtccgact tatgaaacac
     3121 cagcagaaat tcgttcctat aagacactgg gagcagatgc agttggtatg tctacggttc
     3181 ctgaagttat cgtggcagcc cactctggct tgaaagttct gggaatttca tgtatcacta
     3241 actttgcggc tggtttccaa gaagaactca atcacgaaga agttgtagaa gtgactgaac
     3301 gtgttaaagg tgattttaaa ggcttgctta aagcgattct tgctgaattg taagaaaaaa
     3361 gatttaaaag ggggagtgcc tctgtctttt caggattgac tgcctatccg tattaaagaa
     3421 gaaacagagg aatactatga gcttcttcct gctcttataa ctgaaagaag cggaagaata
     3481 ggtgtgtctg atctgatagc cagcattgtg aaagacaaga ttctaggata ctagcattag
     3541 cttcctagcc aagcagacta gtatgataag gagagatgag aatgaattga ctttctgaat
     3601 ttctcagtct tatcatatat agcacaatga gatttcgctt gagtctgctt gtaaataaac
     3661 gaaaagaaag ataagaaata atgaaaattg gtcaacgaat tatgcgcttt ggcataaaaa
     3721 attaagtatc ggagttgtat ctgttgtagt cggctttgat ttctagctcc agctggaatt
     3781 tcagccaatg aagtaaagca agatgtaaca actgaagtgg taataggtgt gctagattct
     3841 aaggaggaat tgaaagagtc agaaaatgat gctccaaaac tagaaactcc tcttagagag
     3901 gagccaagac tagctcctca aacgcttccg gaagcaagtg aagttcttga aaacaaaagg
     3961 gaagagtcaa aagtagagat aacagagcca gctcaagcgg atgacatccg caaggttgtt
     4021 ggggaattag ccaaggatat aagtattact aagttgtata tgacaggtca ttctcttgga
     4081 ggctacctag ctcagattgc agcggttgaa gattaccaaa aatatcctga tttttataac
     4141 catgtattga ggaaagtgac aactttcagt gctcctaaag tgattacttc cagaactgtt
     4201 tgggatgcta agaatggttt ctgagatgtt ggtttggaaa gtcgtaaatt agctgttagt
     4261 ggaaaaatta agcattatgt ggttgataat gacaatgttg tgactccctt gattcataat
     4321 aatcgtgata ttgttacatt tacaggtaat tcacgcttta aacaccgttc tcgtggctat
     4381 tttgaaagtc caatgaatga tattcctaac tttaatattg gtaaacaagc taccttggat
     4441 aaacatggtt atcgtgatcc gaaattggat aaagtgcgat tctttaagaa acaggctcta
     4501 cctcaatctt ctagtcaacc aagcgctgaa ccaatggaaa atattgcctt aggaaaacag
     4561 gttactcaaa gttcgacagc tttcggagga gatgctagaa gagctgtgga tggcaaagtc
     4621 gatggtaact atggtcacaa ttctgtcact catacaaact tccaatctaa gccttggtgg
     4681 caagtagatt tggctaaaga agaaaccatt cgccaaatca atatttacaa ccgaacagac
     4741 actgcccagg atagattggc aaactttgat gtcattcttt tagacagttc tggtaaagaa
     4801 attgagtgaa aacgtataat atctcctaaa gatgtgtcag cacaaattac gattaaccat
     4861 aaaaaagcgc gctatgttcg gattgggcta gaaggctata atgccctcag tcttgcagaa
     4921 gttgaagttt tccgctttat agctacgaat gctgaaacgg cgacacaagt ttctaagcca
     4981 gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca acacagtgga
     5041 gcttacattg cccgctactc cataacttgg gaagaagttc cagtagataa agatggaaac
     5101 caagttgttc gtagtcattc ttgggaagga aacggttgca accagactgc aggttttgtc
     5161 ctcaacttcc caatcaaaga aaatatgaga aatctgcgag ttaagattga gaaaaagacg
     5221 ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttt agctcaaccc
     5281 caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga cgatgatgtc
     5341 ttgtaatctg atggtacaat gacagttagt ttgtctagtt tataagaaag tactatctga
     5401 gcttgaatag aactcaggta gctctctgtg aaagaacaaa attaatactc aatgaaaatc
     5461 aaagagcaaa ctaggaaact agccgcaggt tgctcaaagc actgctttga ggttgtagat
     5521 aagactgacg aagtcagtca catatataat ccaaggcgac gttgacgtgg tttgaagaga
     5581 ttttcgaaga gtataaacag aaaggtagag cgcgtgttct aatttgaaca cgagtagaaa
     5641 acttttctaa aaacaaaaac gaaaggatgg gtaaactgta ttcgctgaac tgaatacggg
     5701 cgactctcct ctaaatcaaa attaagaaag aaaggaattg accccaccct aaaagcagtg
     5761 ggaaaaagat agttggtcta gcgagcatcg ctcactgcgc ccaactccta ttttcccttc
     5821 gctttttgac gggtttggta tctttctcaa tataaaatat aaaataaaga aaggtagagc
     5881 gtgtgttttg atttgaacac gagcggaaaa ctcggaaaat agataatctg actgaaaaat
     5941 caggatttct cgtcggcttc ctaattttca gtcgttttct tctcgctctt tgtatcataa
     6001 attatgtcta tccatattgc tgctcagcag ggtgaaattg ctgataaaat tcttcttcct
     6061 ggggatcctc ttcgtgctaa gtttattgcg gagaatttcc ttgatgatgc tgtttgtttt
     6121 aacgaagtgc gtaacatgtt tggttacact ggtacttaca agggtcactg tgtatctgtc
     6181 atgggaactg ggatgggaat gccatctatt tcgatttatg cgcgtgagtt aatcgtagac
     6241 tacggtgtga agaaattgat tcgtgtggga actgcaggtt ctttgaatga agaggttcat
     6301 gttcgtgaat tagttttggc gcaggcggct gcaaccaact caaacatcgt tcgtaatgac
     6361 tggccacagt acgattttcc acaaattgct agctttgatt tgcttgataa agcctaccat
     6421 atcgccaaaa aacttggtat gactactcac gttgggaacg ttttgtcatc tgatgtcttt
     6481 tactcaaatt actttgaaaa gaatatcgag cttggtaaat ggggagtcaa ggctgtggaa
     6541 atggaagcag cagctcttta ctatcttgct gcccaatacc atgttgatgc gctagctatc
     6601 atgaccatct ctgatagctt ggtcaatcca gacgaagata caactgcaga agaacgtcaa
     6661 aataccttca ctgatatgat gaaggttggt ttggaaacct tgattgcaga ataattatag
     6721 ccaaaaagga agccctttat gaattgtagg acttcctttt cttatccaga aattgatcta
     6781 gttctctctg atttcgaaga atagtgactt tatgtgaata ttcttggcaa agtttttggt
     6841 aattttcttt ttgagttttg ctacgcccat cccaaagaat ccatctgata aactcccact
     6901 caaagcgttc agggcaatct gccgccatac tttctctgac ttttccacgg tatttaagat
     6961 aacgcttaaa ggctctaaag agacaggtca atggcaaaaa attgagaaag atgatttggt
     7021 cagcttcttg cattcgttct tggtagtagc accaagaata attaccatcg atgacccaag
     7081 ctttatgctt ggtgagaaag ttttttatct cggttaacat ccattcgcag tcactgtctt
     7141 gccaaccagg ttgaaattgg agtgtgtcca tgtgcagttt tggaatggag tagtagttag
     7201 ataacttttc tgctagagtt gacttaccag aaccagaata tccgataatt gcgattttca
     7261 ttttctacct tttcctattt ggagacaaaa aaacagcctc tatggactgt ttcttattta
     7321 gcaagtttag ctgaaagacg agctttatcg cggcttgctt tgtttttgtg aatcaaacct
     7381 ttagtttctg ctttatcgat agctgagcta gcagcacgga aaagttcttc agatgggttt
     7441 gcttcgaaag cttttatagc agtacgcata gctgattttt gagctgagtt cttttcgttt
     7501 tgtttaacgt tcaattcagc gcgtttgata gctgatttaa tgtttgccaa tgttcttacc
     7561 tccatattta ctaactatac cattatatat gaaaacttac gttttgacaa gggaaaatta
     7621 cttttttaag taaatttcat cgatttcatg gttcttgctt ttatgaagaa tcacttctgc
     7681 acgatttctg gttggttcaa tataattttg cagatttgtg agattgatac tggtccagac
     7741 ctgatgggca aaggactcca cttccccaat cggcatctga gtaaaacgat aatagtagct
     7801 atcagggtcg ttttgggcta ggctcagcat tttcaagaaa cggtccagat accaactttc
     7861 aatatcatcc actccagcat ctacatagat ggaaaagtca aagaagtcag tgatatagag
     7921 acgatcgttt tgtggatttt gaaagacatt aattccctca acaattacaa aatcagcagc
     7981 tttgacactt tgtttctttt cgggtacgat gtcgtaaact tcatgagaat agacaggaat
     8041 atctacatct tgtccatttt tgatgcggtc caagaagttg agaagagctt ccatatcata
     8101 gctttcagga aatcctttac gatttaaaat cccctgctca atcaaggttt gattgggata
     8161 gagaaaacca tcagttgtaa ccaactcaac cgtagcatct gtaaacgtac gggacagtag
     8221 gatttgaagt aggcgactgg ttgtggattt tccaacggca acactcccag aaaccccaat
     8281 aataaaaggt tgagatttac tttcacgttg gaggaaaatt ccttttgaaa aggctaaatc
     8341 ttccttagtt cgcttgtaaa tctgaatcag atgagccaag gggagataga tatctgtaac
     8401 gtcttggaga ctgatttggt cattaaaact cttgatagat tccaattctt cttctgtcaa
     8461 aggaggtgtt gtctttcgat gtaaagattg ccaagtctgg cggctgattt tttcaaaatg
     8521 taaaaattcg ttggtcattt gttttcccct agatattttg tctatcatac cataaaaagc
     8581 taaaaaaata aagcggtttc ttgaatgtgg taagtcaatg gtatatagta taagtgatca
     8641 atttattgat gaaataggag atagattcta ctgatttata agcttataaa gggggctttt
     8701 tagttcacaa cttataggag gtgtactatg aaaatcttaa aacgttacat attggaactc
     8761 tgttttattt taagttttgc tttacctttt acaaaaggag ccaatgcaga taatggtaga
     8821 tgctttgtgg aaacctatta cggttttact tttttgatgg aacatgctat tgtaacagct
     8881 gtctttatct gttcgatctt aattgctttc ttactaaaaa aacgatgggc gaaatggatt
     8941 gctgctggta gttattgctt tttagtccta tggattgcga cagagggtta tttcttccgc
     9001 atgtcactag aagatttgat acgactttgg acaagtttgg aaatcctgac acaaacgtat
     9061 cagttgggct tttatctaaa cattttgttg ggaattttgt tgataataaa gtattttaag
     9121 gttaagcaat agaaat
//
LOCUS       DAVLSL010000080         8997 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLSL010000080 DAVLSL010000000
VERSION     DAVLSL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.081"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            15..206
                     /gene="secG"
                     /locus_tag="VXN30_001416"
     CDS             15..206
                     /gene="secG"
                     /locus_tag="VXN30_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1358026.1"
                     /translation="MIVIAIFMQPTKNQSSNVFDASSGDLFERSKARGFEAVMQRLTG
                     ILVFFWLAIALALTVLSSR"
     gene            308..2662
                     /gene="rnr"
                     /locus_tag="VXN30_001417"
     CDS             308..2662
                     /gene="rnr"
                     /locus_tag="VXN30_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1358027.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            2625..3092
                     /gene="smpB"
                     /locus_tag="VXN30_001418"
     CDS             2625..3092
                     /gene="smpB"
                     /locus_tag="VXN30_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1358028.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            3108..3968
                     /gene="tehB"
                     /locus_tag="VXN30_001419"
     CDS             3108..3968
                     /gene="tehB"
                     /locus_tag="VXN30_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1358029.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            4041..4994
                     /gene="coiA"
                     /locus_tag="VXN30_001420"
     CDS             4041..4994
                     /gene="coiA"
                     /locus_tag="VXN30_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1358030.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKGKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            5013..6815
                     /gene="pepF"
                     /locus_tag="VXN30_001421"
     CDS             5013..6815
                     /gene="pepF"
                     /locus_tag="VXN30_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1358031.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            6817..7530
                     /locus_tag="VXN30_001422"
     CDS             6817..7530
                     /locus_tag="VXN30_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1358032.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            7597..8538
                     /gene="prsA"
                     /locus_tag="VXN30_001423"
     CDS             7597..8538
                     /gene="prsA"
                     /locus_tag="VXN30_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1358033.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(8657..>8997)
                     /locus_tag="VXN30_001424"
     CDS             complement(8657..>8997)
                     /locus_tag="VXN30_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1358034.1"
                     /translation="LEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRK
                     GNSQDNGRMESFFGILKSEMFYGYEQTFKSLNQLEQAIIDYIDYYNNKRIKIKLKGLS
                     SVQYRTKSFG"
BASE COUNT         2880 a         1668 c         1979 g         2470 t
ORIGIN      
        1 tagtattatc tgttgtgatt gtgattgcaa ttttcatgca accaaccaaa aaccaatcca
       61 gcaatgtatt tgatgccagt tcaggtgatt tgtttgaacg cagtaaagct cgcggttttg
      121 aagctgtaat gcagcgtttg acagggattt tagtcttttt ctggctagcc attgccttag
      181 cattgacggt attatcaagt agataagaaa ataatgggca ggactaggtc tttgcctctt
      241 tttattttta aaggatgttt gagaaggttt tacagtaaaa gaaaattaaa aaatctagaa
      301 agaaaatatg aaagatagaa taaaagaata tttacaagac aagggaaagg tgactgttaa
      361 tgatttggct caggctttgg gaaaagacag ttccaaggat tttcgtgagt tgattaaaac
      421 cttgtcctta atggaaagaa agcaccagat tcgctttgaa gaagatggta gtctgacatt
      481 agaaattaag aaaaaacatg agattaccct caaggggatt tttcatgccc ataaaaatgg
      541 ctttggcttt gttagtctgg aaggcgagga ggacgacctt tttgtaggga aaaatgatgt
      601 caactatgct attgatggtg ataccgtcga ggtagtgatt aagaaagtcg ctgaccgcaa
      661 taagggaaca gcagcagaag ccaaaattat tgatatccta gaacacagtt tgacaacagt
      721 tgtcgggcaa atcgttctgg atcaggaaaa gcctaagtat gctggctata ttcgttcaaa
      781 aaatcagaaa atcagtcaac cgatttatgt taagaaacca gccctaaagt tagaaggaac
      841 agaagttctc aaagtcttta tcgataaata cccaagcaag aaacatgatt tctttgtcgc
      901 gagtgttctc gatgtagtgg ggcactcaac ggatgtcgga attgatgttc ttgaggtctt
      961 ggaatcaatg gacattgtat ctgagtttcc agaagctgtt gtcaaggaag cagaaagtgt
     1021 gcctgatgct ccgtctcaaa aggatatgga aggtcgtctg gatctaagag atgaaattac
     1081 ctttaccatt gacggtgcgg atgccaagga cttggacgat gcagtgcata tcaaggctct
     1141 gaaaaatggc aatctggagc ttggggttca catcgcagat gtttcctatt atgtgaccga
     1201 gggttctgcc cttgacaagg aagcccttaa ccgtgcgact tctgtttacg tgacagaccg
     1261 agtggtgcca atgcttccag aacgactatc aaatggcatc tgctctctca atccccaagt
     1321 tgaccgcctg acccagtctg ctattatgga gattgataaa catggtcgtg tggtcaacta
     1381 taccattaca caaacagtta tcaagaccag ttttcgtatg acctatagcg atgtcaatga
     1441 tatcctagct ggcgatgaag aaaagagaaa agaataccat aaaattgtac caagtatcga
     1501 actcatggcc aagcttcatg aaactttaga aaacatgcgt gtgaaacgtg gagctctcaa
     1561 ttttgatacc aatgaagcga agattttagt ggataaacaa ggtaagcctg ttgatatcgt
     1621 tcttcggcag cgtggcattg ccgagcggat gattgagtct tttatgttga tggctaatga
     1681 aacagttgcc gaacatttca gcaagttgga tttgcctttt atctatcgaa ttcacgagga
     1741 gcctaaggct gaaaaggttc agaagtttat tgattatgct tcgagttttg gcttgcgcat
     1801 ttatgggact gccagtgaga ttagccagga ggcgcttcaa gacatcatgc gtgctgttga
     1861 gggagaacct tatgcagatg tattgtccat gatgcttctt cgctctatgc agcaggctcg
     1921 ttattcggag cacaatcacg gccactatgg actagctgct gactattata ctcactttac
     1981 cagtccaatt cgtcgttatc cagaccttct tgttcaccgt atgattcggg attacggctg
     2041 ttctaaggaa atagcagagc attttgaaca agtgattcca gagattgcga cccagtcttc
     2101 caaccgtgaa cgtcgtgcca tagaagctga gcgtgaagtt gaagccatga aaaaggctga
     2161 gtatatggaa gaatacgtgg gtgaagagta tgatgcagtt gtatcaagta ttgtcaaatt
     2221 cggtctcttt gtcgaattgc caaacacagt tgaaggcttg attcacatca ctaatctacc
     2281 tgaattttat catttcaatg agcgtgattt gactcttcgt ggagaaaaat caggtatcac
     2341 tttccgagtg ggtcagcaga tccgtatccg tgttgaaaga gcggataaaa tgactggaga
     2401 gattgatttt tcattcgtac ctagtgagtt tgatgtgatt gaaaaaggct tgaaacagtc
     2461 tagtcgtagt ggcagagggc gtggttcaaa tcgtcgttcg gataagaagg aagacaagag
     2521 aaaatcagga cgctcaaatg ataagcgtaa gcattcacaa aaagacaaga agaaaaaagg
     2581 aaagaaacct ttttacaagg aagtagctaa gaaaggagcc aagcatggca aagggcgagg
     2641 gaaaggtcgt cgcacaaaat aaaaaggcac gccacgacta tacaatcgta gatacgctag
     2701 aggcagggat ggtcctgact ggaactgaaa tcaagagtgt acgagctgct cgaattaatc
     2761 tcaaggatgg ctttgctcaa gtgaaaaatg gagaagtttg gctgagtaat gttcatatcg
     2821 cgccttacga agagggcaat atctggaacc aggaaccaga acgtcgtcgt aaactcctgc
     2881 tccataaaaa gcaaattcaa aaattggaac aagagaccaa agggacagga atgaccttag
     2941 ttccccttaa ggtctatata aaagatggct acgctaagct tcttttagga cttgccaaag
     3001 ggaagcatga ctatgacaaa cgggagtcta tcaaacgtcg tgagcaaaat cgagatatcg
     3061 cgcgtgtgat gaaagctgtt aatcagcgat aaaaagagga attgaaaatg gaaaaattag
     3121 ttgcctataa acgcatgcct ttgtggaata aacaaacaat gcctgaagct gttcagcaaa
     3181 agcacaatac aaaagttggg acttggggga aaattactgt cttgaaggga gctctcaagt
     3241 ttattgaatt gacagaagaa ggggaagttc tagctgaaca cctctttgaa gcaggggcag
     3301 acaatccaat ggcccaacct caagcctggc accgagtgga agctgccaca gatgatgtgg
     3361 aatggtactt ggaattttat tgtaaacctg aggattattt tgctaaaaaa tacaatacca
     3421 atcctgttca ttcagaggtc ctagaggcca tgcagacagt gaaacaaggg aaagctttgg
     3481 atttgggttg tggtcagggg cgtaattctc tttttctagc ccagcaagat tttgatgtga
     3541 cggctgtaga tcaaaatgga ctagctcttg aaatcttgca aagcattgtg gagcaggaag
     3601 atttggacat gcctgttggc ctttacgata tcaattcagc tagcattgaa caagaatatg
     3661 attttatcgt ttcaacagtt gttctcatgt ttctacaagc ggaccgcatt ccagctatta
     3721 ttcaaaatat gcaggagaaa accagtgttg gtggttacaa ccttatcgtt tgtgccatgg
     3781 acacggagga ttatccttgc tcggttaact tcccattcac ctttaaagaa ggagaactgg
     3841 cagactatta caaggattgg gaattggtta agtacaatga aaatccaggc catttgcacc
     3901 gtcgcgatga gaatggcaat cgtattcaac tacgctttgc gaccttacta gctaagaaaa
     3961 tcaagtaaac acacatgaag attaggaatt ttcctgatct tttttctttt ttacgaatga
     4021 tatagaaaag gagggaattc atgtttgttg cgagagatgc taggggagaa ttggtaaatg
     4081 tgttagagga taaacttgag aagcaagcat acacctgccc agcttgtgga ggccagctcc
     4141 atttgcgtca aggaccaagt gtacggacgc attttgccca taaatcttta aaagactgtg
     4201 attttttctt tgaaaatgaa agtccagaac acctggccaa taaggaatcc ctctatcact
     4261 ggttgaaaaa agagacaaag gttcaattag agtacccgct ttcagaactt aaacagattg
     4321 cggatgtatt tgtaaatggc aatctagctc tagaagttca gtgtagtccc ttgcctcaga
     4381 aagtccttaa agagcgaagt gagggctatc gtagtcaggg ttaccaagta ctgtggttgc
     4441 tgggtcaaaa actgtggctc aaggagcgtt tgactcgtct acagcaaggt tttctttatt
     4501 tcagtcaaaa catgggcttt tatgtttggg aattagacaa gggaaaacaa gttttaagac
     4561 tcaaatacct gatttaccag gatctccgcg gtaaactcca ttatcaaatc aaggaatttt
     4621 cctatggcca aggtagttta ttggaaatat tgcgtcttcc ctataagaaa caaaaaatat
     4681 ctcattttac agtttctgag gacaaggaca tctgtcgcta tatccggcaa caactttatt
     4741 atcaaaatct cttttggatg aaagaacaag cagaagccta tcaaaaggga gaaaatatcc
     4801 tgacttatgg actgaaagaa tggtatccac aaattcgacc aatagtgggc aaatttttcc
     4861 agattgaaca agacttgact agctattatc agcactttta tacctattac caaaaaaatc
     4921 ctcaaaatga ttggcaaaag ctttatccac cagcctttta tcagcaatat ttcttgaaaa
     4981 atatggtaga atagaaagga tggaggaatc taatggtatt acaaagaaat gaaataaatg
     5041 aaaaagatac atgggatcta tcaacgatct acccaactga ccaggcttgg gaagaagcct
     5101 taaaagattt aacagaacaa ttggagacag tagcccagta tgaaggccat ctcttggata
     5161 gtgcggataa cctactagaa atcactgaat tttctcttga aatggaacgc cagatagaga
     5221 agctttacgt ttatgctcat atgaagaatg accaggatac acgtgaagct aagtatcaag
     5281 agtactatgc caaggccatg acactctaca gccagttaga ccaagccttt tcattctatg
     5341 agcctgaatt tatggagatt agcgaaaagc agtatgctga ctttttagaa gctcaaccaa
     5401 agctgcaggt ttatcaacac tattttgaca agctcttgca aggcaaggat cacgttcttt
     5461 cacaacgtga agaagaatta ttggctggag ctggagaaat ctttggttca gcaagtgaaa
     5521 ccttcgctat cttggacaat gcggatattg tgttccctta tgtcctagac gatgatggta
     5581 aagaagttca gctatctcat gggacttaca cacgtttgat ggagtctaaa aaacgtgagg
     5641 ttcgccgtgg tgcctatcaa gctctttatg cgacttacga acaattccaa cacacctatg
     5701 ccaaaacctt gcaaaccaat gttaaggtgc aaaattaccg tgctaaagtt cgtaactaca
     5761 agagtgctcg tcatgcagcc ctcgcagcga attttgttcc agaaagtgtt tatgacaatt
     5821 tggtagcagc agttcgcaag catttgccac tcttacatcg ctatcttgag cttcgttcaa
     5881 aaatcttggg gatttcagat ctcaagatgt acgatgtcta cacaccgctt tcatctgttg
     5941 aatacaattt tacctaccaa gaagccttga aaaaagcaga agatgctttg gcagtcttgg
     6001 gtgaggatta cttgagccgt gtcaaacgtg ccttcagcga gcgttggatt gatgtttacg
     6061 aaaatcaagg caagcgttca ggtgcctact ctggtggttc ttacgatacc aatgccttta
     6121 tgcttctcaa ctggcaggac aatctggaca atctctttac tcttgttcat gaaacaggtc
     6181 acagtatgca ttcaagctat actcgtgaaa ctcagcctta tgtttacgga gattactcta
     6241 tctttttggc tgagattgcc tcaactacca atgaaaatat cttgacggag aaattattgg
     6301 aagaagtgga agacgacgca acacgctttg ctattctcaa taacttccta gatggtttcc
     6361 gtggaacagt tttccgccaa actcaatttg ctgagtttga acacgccatt caccaagcag
     6421 atcaaaatgg ggaggtcttg acaagcgatt tcctaaataa actctacgca gacttgaacc
     6481 aagagtatta tggtttgagt aaggaagaca atcctgaaat ccaatacgag tgggctcgca
     6541 ttccacactt ctactataac tactatgtat atcaatattc aactggcttt gcggccgcct
     6601 cagccttggc tgaaaaaatt gtccatggta gtcaagaaga ccgtgaccgc tatatcgact
     6661 acctcaaggc aggtaagtcg gactatccac ttaatgtcat gagaaaagct ggtgttgata
     6721 tggagaagga agactacctc aacgatgcct ttgcagtctt tgaacgccgt ttaaatgagt
     6781 ttgaagccct tgttgaaaaa ttaggattgg cataaaatgg ttgaatcgta tagtaagaat
     6841 gctaaccata acatgcgtcg tcctgtcgtc aaagaagaaa ttgtagactt gatgcgtcag
     6901 cgtcaaaagc aggtcacagg tttcttgaaa gaattggaag actttgcccg caaggaaaat
     6961 attcctatta ttccccatga aacggttgct tatttccgtt ttcttatgga aaccatgcag
     7021 cctaaaaata ttctggaaat tgggacggct atcggttttt cagctctctt gatggctgaa
     7081 catgcgccaa atgctaagat tacaactatt gatcgtaatc cagaaatgat tggttttgcc
     7141 aaggaaaatt ttgcccagtt tgacagtcgc aagcaaatca ctctcctaga gggagatgcg
     7201 gttgatgtct tatctacact gacagagtct tatgatttcg tctttatgga ttctgccaag
     7261 tctaaataca tcgtctttct gccagaaatc ctcaaacatt tggaagttgg tggtgtggtt
     7321 gtcttggatg atatttttca aggtggtgat gttgccaagg atattatgga agtccgtcgt
     7381 ggtcagcgaa ccatttatcg aggccttcaa aaattatttg atgcaacctt agacaatcca
     7441 gaactcaccg caacattagt gcctttagga gatggtattc tcatgcttcg taaaaatgta
     7501 gcagatgttc aactgtctga aagcgaatga ttttcagaaa aatttaagaa aaaatagtaa
     7561 aatagataga gtaacactta tctcaaagga gtagacatga agaaaaaatt attggcaggt
     7621 gccatcacac tattatcagt agcaacttta gcagcttgtt cgaaagggtc agaaggggca
     7681 gaccttatca gcatgaaagg ggatgtcatc acagaacatc aattttatga gcaagtgaaa
     7741 aacaaccctt cagcccaaca agtcttgtta aatatgacca tccaaaaagt ttttgaaaaa
     7801 caatatggct cagagcttga tgataaagag gttgatgata ctattgccga agaaaaaaaa
     7861 caatatggcg aaaactacca acgtgtcttg tcacaagcag gtatgactct tgaaacacgt
     7921 aaagctcaaa ttcgtacaag taaattagtt gagttggcag ttaagaaggt agcagaagct
     7981 gaattgacag atgaagccta taagaaagcc tttgatgagt acactccaga tgtaacggct
     8041 caaatcatcc gtcttaataa tgaagataag gccaaagaag ttctcgaaaa agccaaggca
     8101 gaaggtgctg attttgctca attagccaaa gataattcaa ctgatgaaaa aacaaaagaa
     8161 aatggtggag aaattacctt tgattctgct tcaacagaag tacctgagca agtcaaaaaa
     8221 gccgctttcg ctttagatgt ggatggtgtt tctgatgtga ttacagcaac tggcacacaa
     8281 gcctacagta gccaatatta cattgtaaaa ctcactaaga aaacagaaaa atcatctaat
     8341 attgatgact acaaagaaaa attaaaaact gttatcttga ctcaaaaaca aaatgattca
     8401 acatttgttc aaagcattat cggaaaagaa ttgcaagcag ccaatatcaa ggttaaggac
     8461 caagccttcc aaaatatctt tacccaatat atcggtggtg gagattcaag ctcaagcagt
     8521 agtacatcaa acgaatagtc caaatcaatg agtcagggaa aaaactcgac ttcaggaaaa
     8581 aatgaagcaa acattcccac aataaaacgc atagtacaag gtttgtactg ccccccaaaa
     8641 aagttagaca attaatttat ccgaaggatt tagttctgta ttgcacagag ctaagtcctt
     8701 ttagttttat cttaattctc ttattgttgt aataatcaat atagtctata atggcttgtt
     8761 ccaattgatt aagtgattta aatgtttgct catagccata aaacatttcg gattttaaaa
     8821 tgccaaagaa agattccatc ctaccgttgt cttggctgtt tcctttgcgt gacatagatg
     8881 cttgaattcc cttactctct aggaagcgat gataagaatc gtgttggtat tgccagcctt
     8941 ggtcactatg gagaatcgta ttctcgtagt gcttctcttt gaatgcctgt tccaaca
//
LOCUS       DAVLSL010000081         8994 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLSL010000081 DAVLSL010000000
VERSION     DAVLSL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.082"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..226
                     /locus_tag="VXN30_001425"
     CDS             <1..226
                     /locus_tag="VXN30_001425"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358035.1"
                     /translation="DPNGSWARQQKVTIDNINRLEKMIEDAVKFDAEDEVAKLYPETN
                     SRVLKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            270..5483
                     /locus_tag="VXN30_001426"
     CDS             270..5483
                     /locus_tag="VXN30_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1358036.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNMDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            5755..6606
                     /gene="ylqF"
                     /locus_tag="VXN30_001427"
     CDS             5755..6606
                     /gene="ylqF"
                     /locus_tag="VXN30_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1358037.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            6593..7372
                     /locus_tag="VXN30_001428"
     CDS             6593..7372
                     /locus_tag="VXN30_001428"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1358038.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7388..8938
                     /locus_tag="VXN30_001429"
     CDS             7388..8938
                     /locus_tag="VXN30_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1358039.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNILITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3005 a         1535 c         1867 g         2587 t
ORIGIN      
        1 cgatccaaac ggttcttggg ctcgacaaca gaaagtgact attgataata ttaatagact
       61 tgaaaaaatg attgaagatg cagtaaaatt tgatgctgag gatgaagttg ccaaattata
      121 tcctgagaca aatagtagag ttcttaaact gaaaaaagca atctttaaag cttatcttga
      181 ccaaaccggt gattttagaa gttcaatttt tgagaataaa aaatagtgtt tactattagg
      241 aaataaaaag tgaaggtgga ggattagaat tgtcattatt aaaaaaagat aaattttcca
      301 ttcggaaaat aaagggtatt gttggttcgg tattcttggg gagtctttta tttgcaccgt
      361 cagttgttgg tgcatcgact tatcattact tagattatag tagtttgaca caaactgaac
      421 gtgatcaact caaacaaggt agacctgatg aatccaaaga gtcatatgct ttggattatg
      481 aaaaagacgc actaccaaac acaggaagtt ctcaatctat tatgactgca ttgggtttgt
      541 tagctatcgg gagtcttatt gtgattatca caaaagataa tagaaataaa aaaatagcta
      601 catttttgat tgtaggggcg acaggtttag ttacactatc aactgcttca gcacttaatt
      661 tgaatgctaa catccatgag tctggacgtg atggtgtgtt gcaaatttct ggttacagat
      721 atgttggtta cttggagctt gatgataaaa ctgtttcgtc ggtttctcca gcatcaactg
      781 tttcgccagt tgagcaaccc aaagttgtaa cagagaaagg cgaaccggaa gttcatgaga
      841 agcctgacta tacgcaacca ataggtgcaa accttgttga gcctgaagtt catgagaaac
      901 tagcatacac tgaaccagta gggacaacag gagtagatga aaatggaaac ttgattgaac
      961 cacctgttaa cgacatccca gaatatactg agccagtagg tacaacagga gtggatgaaa
     1021 atggaaactt gattgagcca cctgttagcg acattccaga gtatactgaa ccaatatcta
     1081 cagtttcaga agttgcatca gaaagagaag agttgccttc tttacatacc gatattcgga
     1141 cagagactat tcctaaaacc actatagaag aatctgatcc tagcaaattt ataggcgatg
     1201 attctgttag acaagttggt gaggatggcg aacgtcaaat tgttactagc tatgaagagt
     1261 tacatggcaa aaaaatcagt gacccagttg aaacagtaac tattttgaaa gaaatgaagc
     1321 ctaaaattct tgtaaaaggt accaaagaaa aacctaaaga aaaaacggct cctgttttga
     1381 ccttagatag aacaaataca aatgtattaa accgatccgc aactctttct tatcatttag
     1441 ttaatacaga tggagtcaca atcaataaaa ttactgcgac aattaaagat ggaaatgaaa
     1501 ttgtcaaaac agtagatttg acttcagagc aattagataa gcaggtagaa gatttaaaat
     1561 tctacaagga ctataagata gagaccacta tgacctatga ccgtggcaag ggagaagaaa
     1621 ctgcaacatt agaagagaaa ccacttcgct tggatttgaa aaaagttgaa attaaaaata
     1681 tcgcatctac taatttagtg aaggtaaatg acgatggtac tgaaacacct agtgatttta
     1741 tgactgaaaa accttcagat gaagatgtga agaaaatgta cttaaaaatt actagtcgtg
     1801 ataataaagt aacacgttta gcagttgata aaattgaaga agtgactgaa gaaggtaaga
     1861 aactttataa aatcactgcc gaagctcaag atttgattca acatacagac ccaacgaagg
     1921 ttcgtaacaa gtatgttcat tatattgaaa aaccagttcc aaaagttgat gacgtttatt
     1981 ataactttaa agagttagta gatgccatga atgctgataa aaatggtaca tttaaaatcg
     2041 gagcagattt gaatgcaacg aatgtaccaa cacctaacaa acaatatgtt ccaggtacgt
     2101 ttaagggaca tctatcaagt gttgatggta aacaatatac aattcataac attgcccgtc
     2161 ccttatttga ccgtgttgaa aatggttctg tgaaaaatat taatttaggt aatatggata
     2221 tcaatatgcc ttgggctgac ggaatagcac ctgttgccaa tatggtgaaa aatgcaactg
     2281 ttgaagatgt taaggtaact ggtaatgtag ttgctaataa caatattgcg ggtatcgtca
     2341 ataaaatcga tagtggtggt cagttgacaa atgtagcctt catcggtaat cttacaggtg
     2401 ttggtgataa aggtcaatat atggctggca ttgccggtga gatttggaga ggtaatcttg
     2461 caaaagcata tgtcgaggct gatattgttg ctaacagagc ccgtataggt ggtttagttg
     2521 ctaaaacaga taatggtaac gactctatgg gaattggtaa atacggttca attcgtaaat
     2581 cagtcaccaa aggaaccatt aagactaaag ttttgtttga aacaggtgga tttatcaata
     2641 gcaatcttcc ttttggtaaa cttgaagata atatttcaat gatgagagtt gaaaatggtg
     2701 aagagttctt tggttcttcc gatttggact acgatggtgg ttatttcaca aatggctggt
     2761 tagagcgtaa ctttgttgtc aaaggtgtca gttcaggtaa gcattcatac aaacgttctc
     2821 gtgacaaaat taaagaaatt agtcaagatg aagctaataa gagaattgca aattttggac
     2881 ttactgctga taaatacgaa atcaacgaac ctgtggtcaa tcgcttaaat cgtttaacca
     2941 gaagagaaga tgaatataag tcaactcaag actacaaatc cgaacgtgat ttagcttatc
     3001 gtaatattga gaaacttcaa ccattttata ataaagaatg gattgtcaac caaggtaaca
     3061 aacttgcaga agattcaaac ttagctaaga aagaagtcct ttctgttacg gggatgaagg
     3121 atggtcaatt cgtgaccgat ttatcagata ttgataagat tatggttcac tatgctgatg
     3181 gtactaagga agaaatggat gttactaaga atactgattc taaagtccaa caagtgcgtg
     3241 aatatagtgt atctggtctt ggtgatgttg tttatacacc gaatatggtt gttaaaaatc
     3301 gtgataaatt gattgctgat gttaaatctc aattatcaag tgtggaattg atttcacaag
     3361 aagtaagaga tttaatgagt agacgtgata aacctgcaga gaatacggat gaacgtaaga
     3421 atggttatat taaagatcta tacttagaag aaagctttgc cgaagtgaag caaaatctag
     3481 ataagttagt caaatccttg gtcgaaaatg aagaccatca attgaatggt gatgaagctg
     3541 cgataaaatc acttctcaag aaagtggaaa ccaataaagc taaaatcatg atggctctga
     3601 cttatctcaa tcgttactat gatatcaaat acggtgacat aagtataaaa aacatcatga
     3661 tgttcaaacc tgatttttat ggtaaaacac caagtgttat tgacagactc atcaacatcg
     3721 gttcaagtga aaagaacttg aaaggggatc ggactcagga tgcttaccgt gaaatcatcg
     3781 caggtaatac tggtaaaagt aatttgcgta acttcttaga atacaacatg cgtctgttta
     3841 cagaggataa agacatcaat gattggttta tccactcagc taagaatgtt tatgtttcag
     3901 aacctaagac aacaaataca gagttgaaag ataaacgtca tcgtgtgttc gatggtttag
     3961 ataacggtgt tcatggtcgt atgattttac cacttctaac gcttaaagat gcccatatgt
     4021 tcttaatttc aacatataac acgatggcgt acagttcatt tgagaaatat ggaaaacata
     4081 cagaagaagc tcgtaatgag ttcaaaacta agattgatga agttgcacat gctcaacaaa
     4141 cttatttaga tttctggtcc agattagctc tgcctaatgt tcgtgataga cttcttaaga
     4201 gtcaaaatat ggtaccgaca ccagtttggg ataaccagac ctacaatggt tctcctgtag
     4261 gtcgtcgagg atttgacagt aaaggtaacc ctatagctcc aattcgtgaa ttatatggtc
     4321 caacatggcg acaccatgat agagactggc gtatgggcgc tatggcatca atattcccta
     4381 atccaaataa tgatgacaag gtcttgttta tggtgacgga tatgattagc ccatttggta
     4441 tttcagcctt tactcacgaa acaacacacg taaacgatag aatgctttac ttcggtggtc
     4501 ataagcatcg tcaaggtaca gacgttgaag cctatgctca aggtatgttg caaacacctg
     4561 acagctcaac gactaatggt gaatacggcg cacttggaat taacatggct taccatcgtc
     4621 caaatgatgg taaccaatgg tacaacccag atcctgacaa attaaagaca cgtgatgaca
     4681 ttgaccgcta catgagaaac tacaatgaag ctatgatgtt gcttgatcat gtagaagctg
     4741 atgctgtact tcctaaaatt aaaggagaca atagtaagtg gttcaagaag attgacaaag
     4801 aaatgcgctc taagattcag tataatgatc ttttaggtcc aaaccaatgg gatagtatcc
     4861 gtgatttgaa agatgaagaa aaggtaatga ctttatctag cgtcaatgat ttagtggata
     4921 ataacttcat gacaaaacat ggaaatccag gtaatggacg ttatagacca gaagatttta
     4981 caccaaactc agcttacgta aatgtcaata tgatggcagg aatttacggt gggaatacga
     5041 gtcaaggtgc tcctggttca ttatcattta agcacaatgc tttccgtatg tggggctatt
     5101 atggttatga aaatggcttt attagttatg tatcaaataa atacaaagca gaagcagata
     5161 aaaacaacca cggtttacta agcgataagc taataattaa taaagtatcg aaaggaaact
     5221 ttaatactct tgaagagtgg aagagacatt ggtacgggga agttcttgct aaagctaaga
     5281 aaggttttga ggctatcgat attgatggcg tccatatcag taactatgat gagttaaggc
     5341 ctttatttga taaagcagtt gaagaagatt tgaagaaacc agatgacttc tctcacactg
     5401 ttgctcttaa atctaaggtc ttcaaagccc ttcttaaaaa cacagacggt ttctttaata
     5461 aactgtttaa agaagacatt taaatgttta gaatatagaa ggaacagttt tttgctccct
     5521 ctgaaaagtc atcatttgat ggcttttttc tatatagggt aaaagatagg ctaaaagata
     5581 gggtaaaagg ctatcatcgg acaaaataaa gaaggcatga tataatataa agtagatttc
     5641 tatgtcataa aacaagaact gtttggacat cattcatttg aaaactctct atgttcaaac
     5701 tatagtaaaa taaaataggg gatctaaatc cttgctacga aaggaaaaaa ctcaatggct
     5761 actattcaat ggtttcctgg tcacatgtct aaagcgcgtc gacaggtgca ggaaaattta
     5821 aaatttgttg attttgtgac gattttagta gatgcacgct tgcctctatc tagtcaaaat
     5881 cctatgttga ccaagattgt tggtgataaa ccaaaactct tgattttaaa caaggccgac
     5941 ttggctgatc cagcaatgac caaggaatgg cgtcagtatt ttgaatcaca aggaatccag
     6001 acgctagcta tcaactccaa agagcaagtg actgtaaaag ttgtaacaga tgcggccaag
     6061 aagctcatgg ctgataagat tgctcgccag aaagaacgtg ggattcagat tgaaaccttg
     6121 cgtaccatga ttattgggat tccaaacgct ggtaaatcaa ctctgatgaa ccgtttggct
     6181 ggtaaaaaga ttgctgttgt tggaaacaag ccaggggtca caaaaggtca acaatggctt
     6241 aaaaccaata aagatctgga aatcttggat acaccgggga ttctctggcc taagtttgag
     6301 gatgaaactg ttgcacttaa gttggcattg actggagcta tcaaggatca gttgcttcct
     6361 atggatgagg ttaccatttt tggtatcaat tatttcaaag aacattatcc agaaaagctg
     6421 gctgaacgct tcaaacaaat gaaaattgaa gaagaagcgc ctgtgattat tatggatatg
     6481 acccgcgccc tcggtttccg tgatgactat gaccgttttt acagtctctt cgtgaaggaa
     6541 gtccgtgatg gcaaactcgg taactatacc ttagatacat tggaagacct cgatggcaac
     6601 gattaaagaa atcaaagaac tccttgtgac agtcaaggag ttagaaagcc ctattttttt
     6661 agagcttgaa aaggataatc gctcaggagt tcaaaaggaa atcagcaagc gtaaaagagc
     6721 cattcaagct gaattagatg aaaatttgcg cttggaatcc atgctttctt atgaaaaaga
     6781 actttataag caaggattga ccttaattgc aggtattgat gaggttggtc gtggtcctct
     6841 tgctggtcct gtagtcgctg cggccgttat tttacctaaa aattgtaaga ttaaaggtct
     6901 caacgacagc aagaaaattc ctaaaaagaa acatctggag attttccaag ccgttcaaga
     6961 ccaagccttg tcgattggaa ttggtatcat agataatcag gtcatcgacc aagtcaacat
     7021 ctatgaagca accaaactag ccatgcaaga agcaatctcc cagctcagcc ctcaaccaga
     7081 gcaccttttg attgatgcca tgaaactgga cttgcccatt tcacaaacct ccattatcaa
     7141 aggagatgcc aactccctct ctatcgcagc agcatctata gtagccaagg taacacgtga
     7201 tgaattgatg aaagaatacg atcagcagtt ctctggctat gatttcgcta ctaatgcagg
     7261 atatggcaca gctaaacatc tggaaggcct cacaaaacta ggagttaccc caattcaccg
     7321 aaccagcttt gaacccgtta aatcactggt tttaggtaaa aaagaaagtt aattgaaagg
     7381 aaataccatg gaggaacagt cggaaatagt ccgttctaag aaagaattcg cctttgcatc
     7441 cagcactata ctatcccaag ttggtcgagg aatcattgtc ggcctcatcg ttggaattat
     7501 cgtcggatcc tttcgtttct taattgaaaa gggcttccac ctgatacaag gagtttatca
     7561 agatcaaggg tacttagtgc gcaatctttt tgtactggtt ttgttttata tactcatctg
     7621 ttggctcagt gccaaactaa cacggtcaga aaaagatatt aaaggctcag gaattcctca
     7681 agtcgaagcc gaactgaaag gcctcatgtc cctcaactgg tggggcattc tttggaaaaa
     7741 atatgtgcta ggtattcttg ctattgccag tggactcatg ctgggtcgag agggacccag
     7801 cattcaactt ggagcagttg gtggtaaagg aattgccaag tggctcaaat ccagtccagt
     7861 agaggaacgt tccttgattg ccagtggagc tgcagcaggt ttagccgcag cctttaatgc
     7921 tcctattgca gcacttctct ttgttgtaga agaagtctat caccattttt cgcgcttttt
     7981 ctgggtctca actctagcag ccagcatcgt agcaaacttt gtgtctctac tcatgttcgg
     8041 tttgacacca gtattggata tgccagataa cattcctccc atgaccctag accagtattg
     8101 gatatatctc gtcatgggaa ttttccttgg attttcaggt tttctctatg agaaagctgt
     8161 attaaacgtt ggaagagttt atgacttgat tggtcaaaaa atccatttgg atagggctta
     8221 ttatcccatc ttggctttta tccttatcat accagtcgga atcttcttac ctcaaatcat
     8281 tggtggcgga aatcagcttg tcctttcttt aactgaacaa aattttagtt tccaagtttt
     8341 attagcttac tttttaatcc gctttatttg gagtatgatt agctatggaa gtggactgcc
     8401 aggaggaatt ttcctcccca ttttagctct tggttctttg cttggtgcct tagttggtgt
     8461 tatctgtgtc aatcttggac ttgtcagtca agagcaattc cctatatttg tcattctagg
     8521 aatgagtggc tattttggag ccatatcaaa agctccctta accgctatga tcctcgtaac
     8581 tgagatggta ggagatattc gcaaccttat gccacttggt cttgtcactc ttgtttctta
     8641 tattatcatg gatttgctca aaggtacgcc agtctatgaa gccatgctgg aaaaaatgct
     8701 tccagaagaa gtatctagcg aaggagaagt tacacttatc gaaataccag tttctgataa
     8761 aattgctggg aaacaagttc atgaactcaa cttaccacac aacatcctca tcacaactca
     8821 agtccataat ggcaagagcc aaacagttaa cggctcaacc agaatgtatc tgggtgatat
     8881 gattcacctg gttattccaa aaagtgaaat tggaaaagtc aaagatttgt tgttgtagta
     8941 tgatcattta cataatttat gttatgtaaa tgatcggttt gatttattta gaaa
//
LOCUS       DAVLSL010000082         8840 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLSL010000082 DAVLSL010000000
VERSION     DAVLSL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.083"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            37..3054
                     /locus_tag="VXN30_001430"
     CDS             37..3054
                     /locus_tag="VXN30_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEV1358040.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            3069..3797
                     /locus_tag="VXN30_001431"
     CDS             3069..3797
                     /locus_tag="VXN30_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV1358041.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRAVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARLTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            3810..5576
                     /locus_tag="VXN30_001432"
     CDS             3810..5576
                     /locus_tag="VXN30_001432"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1358042.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGESVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            5722..6873
                     /locus_tag="VXN30_001433"
     CDS             5722..6873
                     /locus_tag="VXN30_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1358043.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            7018..7902
                     /locus_tag="VXN30_001434"
     CDS             7018..7902
                     /locus_tag="VXN30_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1358044.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            8301..8552
                     /locus_tag="VXN30_001435"
     CDS             8301..8552
                     /locus_tag="VXN30_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1358045.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            8540..8830
                     /locus_tag="VXN30_001436"
     CDS             8540..8830
                     /locus_tag="VXN30_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358046.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
BASE COUNT         2958 a         1414 c         1890 g         2578 t
ORIGIN      
        1 ttgaggaagt tgtatgtaaa tattgaggaa ataaaaatgg aaagtaaaaa aatagcaaaa
       61 cagattttaa tagctacggc tgttttaaca agttttttag gaagtaattt ggtttatgca
      121 gatgtagttc aaagtaattc taataacaga gctagtactg aaactgctag agtaacaggg
      181 aataacttag aaaaacttat tactaaagat aaagaaattg ataaagagat gacatatctt
      241 tcagatatgg attggtcttc tgcaactcat ggtgatatag ataaaacaaa aacagtacaa
      301 aaagatgctc catttacaac aggaaataaa ggagagcata ctaagatttc attgctcact
      361 tctgatgata aagttaaata ttttgataaa ggaataggaa cagtcgcaga ttctccatca
      421 gtaatcagct atgatatcag cggtcaaggg tttgaaaaat ttgaaactta tataggaatt
      481 gatcaaagtg ctaatagttc tagatcagac catgcagtag tggatagaat agaaatagaa
      541 attgatggaa aggttgtcta ttctagtagt gtaactaatc cagaaggatt cagatataat
      601 acccaggcac aatttattag tgtgacaatt ccacaaaacg ctaaaaaaat atccttaaaa
      661 tcattcgcag gagaacatac atggggtgat gaggttgtat ttgctgatgc taaacttatt
      721 aaaactgtta gtactcaaac tataactcca gatcttctta ataaaggaat taatggagga
      781 gtatatcttt ctgatttaga atgggttgat gctactcatg gtgatgacga taaatcaaaa
      841 acagttcaga aagataaacc gtttacacct ggtaataacg ggtctaataa taaaataaaa
      901 ttactaattg acggtaagga agtcgaattt aataagggat tgggaactgt agcaagtaac
      961 ccgtctagta taaaatatga tgtttcgggt gctaatgtta caagatttat ttcctatgtc
     1021 ggaattgata ggagtgcaaa tcatttaaat tcagattacg cagatattca aaaatttgaa
     1081 gttgttgcgg atggtaaagt catttactca tcagattcaa aatatccaaa aggaattaag
     1141 tatgatacat cggcatttct ggttgatgtt gaaattccca aagatacaca gacaatagaa
     1201 cttaaatcat attcagggaa acatacatgg gctgacgaat tggttttagg tggagccttg
     1261 tttatggcaa atggtaaatt taagaatcct aatgattggt cagaagttga taaacgtcgt
     1321 gaaattaata atgaacatcc gttacttatg atgcctttat atgctaacgg agaagagttt
     1381 aaccaaggga aatatacttt ttggggagga gatacattaa caggtaagtg ggagaatata
     1441 ccagatgact taaaaccata tactgttata cagttacatc cagatgatct tcctaaaaga
     1501 gatggagctg ctagagattt ttatgaacat atgttagaag aagcagcgaa atatgtaaat
     1561 cctaagactg gaaaaaatga accaattcca gttattctga cggtttacac tgctggtaat
     1621 atgccttatt atacttcggc acattggtta agtaccagct ggattgataa aatgtatcaa
     1681 aaatatccaa atttgcatgg tattttcagt acagagaatt attggatttg ggcaaatgat
     1741 attgaaaata aggcggcaga ttacttaaaa gtatcagcta aaaatggagg atattttata
     1801 tgggctgaac aaaataatgg ttctgcaata gaaaaagcat ttggaaaaaa cggtaagata
     1861 gcatttcaga aatctgttga taagtactgg aagaacctta ttttcatgtt caaaaataca
     1921 cctgctgctg agggaaatga ttcaactaca gaaagttata tgaagggact ttggctttca
     1981 aatcatactt atcaatgggg tggtttgatg gatacttgga agtggtatga gacaggtaaa
     2041 tggaaactgt ttgcaagtgg taatatcgga aaaagtcaag gagatagaca atggttgact
     2101 gaaccagagt ctatgctagg agaagaagct ttaggggttt accttaatgg aggtgtagtt
     2161 tataattttg aacacccagc ttatacctat ggtgtaaata ataaagaaag cttactcttt
     2221 agcgaagtaa taaaagaatt ttttagatat gtaatagcgc atccagcacc atccaaagaa
     2281 aaggtacttg aggatacaaa agtattcata catggagact attcaaataa aggaaatggt
     2341 aagttctttg taaacgtaaa tactgataga gaacaaactc cattatacat gactggtcgt
     2401 tataatgtta ttccagctat tccaggtgtt ttaaaaacag ataaattgaa agagtctgtt
     2461 tctggtagcc gtattcagat taaggaaatc acttctccag aatttagctc tactcaggct
     2521 agaaaagaat acttaaataa gctgtatcct atgaattatg aaggagatat ctttgctcag
     2581 aaattagata atagatggtt tgtctataat tacaaggtaa atgagaatgt taaacagact
     2641 gggaaattaa aatttaattc tttagaaatg gatgttgaat ttgaacctca tacctatggt
     2701 atttttgaga gaataagtaa tggattgaaa gtgaatttaa ataattttag aacaaataaa
     2761 gattcactat ggtctaatgc tcaagatgca aatcaggcaa agaaactacc acaattgacc
     2821 aagaaaggtg cgattaagtg gattgaagaa cattatatca aagatactca gtttggagag
     2881 aaaagggtta caaagatagt attaagaggt atagataaac ttcctactat tcattcatta
     2941 tcaggaacaa ataattccta tgatcagcca tctctcaatt ttgatcagaa aaatcatatg
     3001 gttactatta ccattaacag taacggaaat cttgaattcg aacttcattt ttagaaagga
     3061 gtgagtttat gtctcaagat gaaaaattaa ttcgtgaaca gatttgtgat gtttgtcata
     3121 agatgtggca acttggttgg gttgctgcta acgatgggaa tgtatctgtt cgattagatg
     3181 aggataccat tcttgcaaca cctactggta tcagcaaaag ttttattaca ccagaaaagc
     3241 tggtgaagtt aaatcttaaa ggagagattt tagaagcaga aggtgattac tgtccttcta
     3301 gtgaaattaa aatgcacatt cgttgttacg aagaacgtga ggatgtccgt gcggttgttc
     3361 atgcccatcc accgattgca acaggatttg ctcttgcaca tattccttta gatacttatt
     3421 cactaattga gagcgcgatt gtggttgggg caattcctat taccccattt ggagtaccgt
     3481 ctacaatgga agtgccagaa gcaattacac cttatctgcc cgatcatgat gtcatgctat
     3541 tagaaaatca tggagctctg actgtcggaa gcgatgtcat tacagcatac taccgtatgg
     3601 aaactttaga attagtcgca aagacaacct tccacggaag aatgttactt tctacaaagg
     3661 gcattgagga gcaagaaatt gctcgtctga ctttagaacg tctattctca atgcgagaaa
     3721 attataaggt tacaggtcgt cacccaggct accgtaaata taatggagat ggtagtatga
     3781 aagaaacaga aaaataagag gaaaatagta tgattcaaca tccacgtatt ggtattcgtc
     3841 cgactattga tggtcgtcgt caaggtgtac gcgaatcact tgaagtgcaa acaatgaaca
     3901 tggctaaaag tgtggcagat ttgatttcaa gcacattgaa atatccagat ggggaatctg
     3961 tggaatgcgt gatttctcca tctaccattg gtcgtgttcc agaggctgca gcttcccatg
     4021 agttgtttaa aaaatcaaat gtttgcgcaa caattacagt tacaccatgc tggtgttatg
     4081 gtagtgaaac tatggatatg tctccagata ttcctcatgc tatttgggga tttaatggga
     4141 cagaacgccc aggagctgtc tatcttgcag ctgtactagc ttcacatgct caaaaaggga
     4201 ttccagcctt tgggatttat ggtagagacg tccaggaagc tagtgataca gatattccag
     4261 aagatgtcaa agaaaaactt ttacgttatg cgcgggcagc tcttgcaact ggcttgatga
     4321 gagacactgc ttacctatca atgggtagtg tttcgatggg gattggtggt tctattgtaa
     4381 atccggattt cttccaagaa tacttaggaa tgcgaaatga atcggtagat atgacggagt
     4441 tcacgcgccg tatggaccgt ggtatttacg accctgaaga gttcgaacgt gcgctcaaat
     4501 gggtgaaaga aaacgtaaaa gaaggattcg accataaccg tgaagacctt gttttaagcc
     4561 gtgaagaaaa agatagacaa tgggaatttg ttattaagat gttcatgatt ggacgtgact
     4621 taatggttgg taacccaaga cttgctgaac ttggttttga ggaagaagca gttggtcacc
     4681 atgctttagt agctggtttc caaggtcaac gtcagtggac agaccatttt ccaaatgggg
     4741 actttatgga aactttcctc aatactcagt ttgactggaa tggtattcga aaaccatttg
     4801 tatttgctac agagaatgat tcactaaatg gtgtgtctat gctctttaat tatctattaa
     4861 caaatactcc acaaatcttt gctgatgtgc gtacttattg gagtccagag gctgttgaac
     4921 gtgtaacggg acatacttta gagggttgtg ctgcagctgg cttcttacat ctaatcaact
     4981 ctggttcttg tacattggat ggtacagggc aagctactcg agatggcaaa cctgttatga
     5041 aaccattctg ggagttggaa gaaagtgaag tgcaggctat gcttgaaaat acagacttcc
     5101 caccagcaaa ccgcgaatac ttccgtggag gaggattctc aactcgtttc ttgacgaagg
     5161 gggatatgcc agtaacaatg gtacgtctca atcttctaaa aggggttggt ccagtgctac
     5221 aaattgcaga aggttacaca cttgaacttc ctgaagatgt tcaccatact ttagataatc
     5281 gtacagattc aggatggcca actacttggt ttgctccacg tttgacagga aaaggtgctt
     5341 tcaagtctgt ctatgacgtc atgaataatt ggggagctaa tcacggagcc ataacatatg
     5401 gacacattgg agcagacttg attaccttgg cttctatgtt gagaattcct gtcaatatgc
     5461 ataatgtacc tgaggaagat atctttagac ctaaaaattg gtccttattt ggaacagaag
     5521 atctagaatc agcagactat cgtgcatgtc agttgttggg accactacat aaataaaact
     5581 tgtttatata ggaggtgaac ttacgtccct cctatccttt taaaaagatt tgttaaacaa
     5641 ttcacaaata attgaaaacg aatacaaaaa gtaatataat gatgttaaat agatagcgcg
     5701 gaggcgcagg aggaaaatta tatggctata ttttatgttc cggcagtcaa ccttattgga
     5761 aaaggtgttg taaatgaagt gggtccttat atcaaggaac ttggctataa aaaggcactt
     5821 ttggtgacag ataagtacat cgaaggcagt gatattttac ctaagacttt aaaaccactg
     5881 gatacagaag gaatagaata tgtcatcttt agcgatgtag agccaaaccc tacttgtaaa
     5941 aatgtcacag atggggtagc tgctttgcaa gaacatggct gtgactttat catcagtctt
     6001 ggcgggggtt ctccacagga tgcagctagt tgtatttcta tcatggctac aaatggtgga
     6061 aaaccacagg attatgaagg gcttcataag tctgctaaaa aaggcttgcc agttgtggct
     6121 atcaatacaa cggcaggtac atcagcagaa attaccatta actatgtgat tactgatgaa
     6181 gaacgcaagg ttaagatggt aatggttgac aagaatagcc ttgccttgat ttctgtcaat
     6241 gaccctgaac tcatgctttc caaacctaaa ggcctgactg ctgctactgg tatggatgct
     6301 ctgactcatg ctgttgaagc tttggtaaca cctggtgctt atgatgtaac caagaaactg
     6361 tctattggtg ctattgagct tatcaaggaa tatcttcctc gtgctgtaga aaatggacat
     6421 gatattgaag cgcgtgaagg tatggtcaat gccatcttcc ttggtggtat gagctttaat
     6481 aatgctggtc ttgggtatgt tcactcaatg gctcaccaac tcggtgcagt atataatttg
     6541 ccacatggcg tgtgctgtgc catgttgcta ccagttatag aacgtgaaaa tgctaaacgt
     6601 gtaccagaag ctttccgcaa tgttgccaaa gccttgggac ttcatgtaga aggtaaatca
     6661 gatcaagaat gtgccgatta tgcgattgct gagattgaga agctttctga gacagtaggt
     6721 attcctaaga aactgactga acttggtatt gaagaaaaag atttcgactt tgaatacctt
     6781 tctaagaatg ccttgattga tgcctgcgca ccaggaaatc catttatgcc aaccttagaa
     6841 gaaacgattg ccttttataa agagttattt tagcaagaga ttggacgaca ataggacaat
     6901 tcacctctaa ttattgacat ttcaggaaaa atcgcagtaa aaacggcaca gccttcttta
     6961 aatgtgctat aatactagaa aaatacttgt ggaggttcca ttatggcaat atttttcatg
     7021 atttttctga ttgtttgtgt gctcctattg gtgatagtca cactgagtac agtttatgtg
     7081 gttcgtcagc agtcggtggc gattattgaa cgctttggga aataccaaaa ggttgctaat
     7141 agcggtattc atattcgctt gccttttggg attgactcga ttgcagcacg gattcagttg
     7201 cgcttgttgc aaagtgatat tgtggttgag actaagacca aggacaatgt gttcgttatg
     7261 atgaatgtag cgactcagta ccgtgtcaac gagcagagcg tgacagatgc ttactataaa
     7321 ctcatacgtc cagaatctca gattaaatct tatatcgaag atgctcttcg ctcttctgtt
     7381 ccaaaattaa ccttggatga attgtttgag aaaaaagatg agattgccct tgaagttcaa
     7441 caccaagtag cagaagaaat gaccacttac ggctacatta tcgtgaaaac cttgattacc
     7501 aaagtcgaac cagatgcaga agttaagcaa tccatgaatg aaatcaatgc ggcgcaacgt
     7561 aagcgggtcg cagcacaaga attggcggaa gctgacaaga ttaaaattgt cactgcagct
     7621 gaagcagaag cagaaaaaga ccgccttcat ggtgtgggga ttgcccaaca acgtaaggcg
     7681 attgtggatg gattggcaga gtctatcacc gaactcaagg aagccaatgt tggcatgaca
     7741 gaagaacaaa tcatgtctat cctcttgacc aaccagtatt tggatacctt gaataccttt
     7801 gcctctaaag gaaatcaaac catcttttta ccaaatactc caaatggtgt ggatgatatc
     7861 cgaacacaaa tcttgtcagc ccttcgcgct gagaagaaat aatagactaa tattcttcga
     7921 aaatctcttc aaaccacgtc agcgtcgcct tgccgtatag atgttactga ctttgtcagt
     7981 tctatctgca acctcaaaac agtgttttga gctgacttcg tcagtcttat ctactacctc
     8041 aaaacggtgt tttgagcaac ctgtggctag tttcctagtt tgctatttga ttttcattga
     8101 gtacaaagag cttggcaaca ggctcttttt gtattgcgtt tattaagaaa aacaaaaaac
     8161 atcgatatac atttaactaa aaattattgt atgttatctc ccttaattag gaatgataag
     8221 ggaataacta gaaagatttg tgaatacaaa ctatttctga tatacaaaat atacagtaat
     8281 aatgaatgat gggagatggg atgaaagaat ttcaatttga gagaaagcag cgtttttctt
     8341 tgaggaaata tgcaatagga gcttgttcgg tcttgctagg aacgagttta ttttttgctg
     8401 gtatgggtgc tcagcctgta caggatacag aaacgagttc agcactaatt tcaagtcatt
     8461 atttggatga gcaggattta tctgaaaagc tgaaatctga gttgcaatgg tttgaattag
     8521 aaaacaagct tttgaactta tgggagcatt aggttactat gaaggatttg ttccttatgt
     8581 ttcaaatcaa tacaaaaacc aagctgaaga agaatacaaa ccgctatctg ataaatatat
     8641 tttcgaaaaa atcttaggaa aaacatatgc agccttcaaa aaagatcaaa ttaatgagcg
     8701 tgtcgagaaa ttaggtaagt taaaacctat tacaataaat tacaacggaa aatcagaagt
     8761 aattgatagt aaagaaaaat tacaagagct tatgaataaa gccgttaaag acgaagtggc
     8821 tcaaatatag taaaatgaaa
//
LOCUS       DAVLSL010000083         8797 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLSL010000083 DAVLSL010000000
VERSION     DAVLSL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.084"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..155
                     /locus_tag="VXN30_001437"
     CDS             <1..155
                     /locus_tag="VXN30_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1358047.1"
                     /translation="IEYIKARNIKTSSDIELESDVDGDKSDNLPVDIKILGQHIEVYS
                     VPKKQS"
     gene            355..3630
                     /gene="rexB"
                     /locus_tag="VXN30_001438"
     CDS             355..3630
                     /gene="rexB"
                     /locus_tag="VXN30_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1358048.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3627..7277
                     /gene="addA"
                     /locus_tag="VXN30_001439"
     CDS             3627..7277
                     /gene="addA"
                     /locus_tag="VXN30_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1358049.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIVLLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7492..8670
                     /locus_tag="VXN30_001440"
     CDS             7492..8670
                     /locus_tag="VXN30_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358050.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLLLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2716 a         1844 c         1878 g         2359 t
ORIGIN      
        1 atattgaata tataaaggca cgtaatatta aaacttcttc tgatatcgaa ttagagtccg
       61 atgttgatgg cgataaatca gacaatctac cagtcgacat taagatactg ggacaacaca
      121 ttgaagtgta ttccgtacct aagaaacaat cataaaactc aaagccttgt taccgaggct
      181 tttttattat gtgattttca gtagccagac ttattctatc taatacaagt aaagctaagt
      241 atttaagatt tagcagatga tgaagaaaag aaaggatggc ctaaatattt taacagattt
      301 acgaggctaa tcatggtata atatagggtg atgaaaaatt ccaacgaggc tgagatgaaa
      361 ttactttata ctgatattcg gacttctttg acagaaattc taacaagaga ggcagaagag
      421 ctagttgcag ctggcaagcg ggtcttctac attgccccca actctctttc ttttgaaaag
      481 gaacgcgccg tgctggaatg cttgtcccag caggcttctt tttcgattac cgtcacgcgc
      541 tttgctcaaa tggctcgtta tctggtcttg aatgatttac cagctaaaac tactcttgat
      601 gatatcggtc ttgggttggc cttttacaaa tgccttgccg aactcgatcc caaggacttg
      661 cgtgtttatg gcgctattaa gcaggatcct caattgatcc agcagttaat tgagctttac
      721 catgagatga ccaaatccca gatgagtttt ttggacttgg agaatttaac agatgaggat
      781 aagagggcgg atttactctt gatttttgag aaagtaacag cctatcttaa tcaaggtcag
      841 ttagcccagg gaagtcagtt gtcccatttg attgaggcta ttgagaatga caaggtaagt
      901 agtgatttta atcaaatcac cttggtcatt gacggcttta ctcgtttttc tgctgaggaa
      961 gagcgggttg tggacttact tcacggcaaa ggtgttgaga ttgttatcgg ggcttatgct
     1021 agtaagaaag cctataccag tccttttagc gagggcaatc tctaccaagc cagcgtaaaa
     1081 tttctccatc atctggcttc taaataccaa acgcctgctc aggactgttc tcaaactcat
     1141 gagaagatgg atagttttga caaggcctct cgtttgttgg agtcttctta tgacttttca
     1201 gaactcgctt tggatgtcga tgagaaagac cgtgaaaatt tacaaatctg gtcttgtttg
     1261 acgcaaaagg aggagttgga gctagtagcc cgtagtattc gtcagaaatt acatgagaac
     1321 tcagacctga gctacaagca ttttcgtatt ctcttggggg atgtagcttc ttaccagtta
     1381 tctctcaaaa ccatttttga ccagtatcag attccttttt atcttggtag aagcgaagcc
     1441 atggctcatc atcccttgac tcagtttgtc gagtctattt tagctttaaa acgttaccgt
     1501 tttcgtcagg aggatttgat taatcttctt agaactgatt tgtatactga cctcagtcag
     1561 tctgatattg atgcttttga gcaatatatc cgctatcttg gtatcaatgg cttgccagcc
     1621 tttcagcaaa tcttcaccaa atcccaccat ggaaaattta atcttgagcg tttgaatgtc
     1681 ctccgcctga gaattttagc acctcttgaa accctctttg ccagccgaaa acaaaaggct
     1741 gaaaacctcc tacaaaaatg gagtgtcttt ctaaaagaag gagctgtgac caagcagtta
     1801 caagatttga caaccacttt ggaagctgta gaacaggaaa gacaagccga agtttggaag
     1861 gctttctgcc atgttttaga acaatttgcg actgtttttg ctggttcaca ggttagtctg
     1921 gaagacttcc tagccttgct ccattctgga atgagtttgt cccaataccg taccattcca
     1981 gcaacagtgg acactgttct ggtgcagagt tacgatttga ttgcaccatt gactgctgac
     2041 tttgtctatg ctattggact aactcaggac aatttaccaa aaatttctca aaacaccagt
     2101 cttctgacag atgaagaaag gcaaaaccta aaccaagcga ccgaagaagg cgttcaatta
     2161 ctgattgcca gcagtgaaaa tctcaagaaa aatcgctaca ctatgctttc cttggtcaat
     2221 tctgctcgta agcagttgtt cttgtcggct ccaagccttt ttaacgaaag tgaaagtaag
     2281 gaatctgcct atcttcaaga gttgatccat tttggattta ggcggagaga gaagaggatg
     2341 aatcacaaag gactgtctaa ggaggatatg gggtcctatc acagtctttt gtctagtctg
     2401 gttgcctatc accagcaggg tgagatgagc gatactgagc aagatttgac ttttgtcaag
     2461 gttctgtcgc gtgtcatagg taaaaaacta gatctgcaag gtctggaaaa tccagctatc
     2521 ccaaccagtc caagcagcaa gaccttagcc aaggacacct tgcaagctct ctatcctgcc
     2581 aaacaggagt tttacctgtc tacgtcgggt ttgacagagt tttatcgcaa tgaatacagt
     2641 tatttcctac gctacgtttt aggcttgcag gaggaattac gtttgcgtcc tgatgcccgt
     2701 agtcacggga atttcttgca tcgtatcttt gaacgcgcct tacagttgcc taatgaagat
     2761 tcctttgacc aacgtctaga acaagctatt caagaaacca gtcaagaacg cgaatttgaa
     2821 gctatttatc aagaaagttt ggaagcccag tttaccaagg aagttttgct tgatgttgca
     2881 cggacgactg gacatattct ccgacacaat ccagccatcg aaaccatcaa agaagaagca
     2941 aattttggtg gaaaagacca agcctttatt caattagaca atggacgcag tgtctttgta
     3001 cgaggcaagg tggaccggat tgaccgtttg aaagctaatg gagcgatagg agtagtagac
     3061 tacaaatcca gtctgactca gttccagttt cctcatttct ttaatgggct caattctcag
     3121 ttaccaacct atcttgctgc cctaaaaaga gaagggaagc agaacttttt cggcgccatg
     3181 tacttggaaa tggctgaacc tgtccaatct ctgatggcgg taaaaagtct ggcaggagca
     3241 gtggtagaag ccagcaaatc tatgaaatac caagggctct tcttggaaaa agaaagcagt
     3301 tatttaggcg aattttataa caaaaacaag gctaatcaac tgacagatga ggaatttcag
     3361 ctcctactgg actacaatgc ctatctttac aagaaagctg ctgagaagat tttagcaggc
     3421 cggttcgcca tcaatcctta tactgaaaat ggcagaagca ttgccccata cgtccagcaa
     3481 catcaggcta ttacaggctt tgaagccaat taccatctgg gccaagcccg tttcctagaa
     3541 aaattggacc tagctgatgg caagcgtctg gtcggagaaa aactcaagca agcttggttt
     3601 gaaaaaataa gagaggagtt aaatcgatga agcttattcc ctttttaagt gaggaggaga
     3661 ttcaaaaact gcaagaagca gaagcaaatt cgagcaagga acagaagaaa actgccgaac
     3721 aaatcgaagc tatctacact tctggtcaga atatcctggt ctcagcatcg gctggttctg
     3781 gaaagacctt tgtcatggca gagcgcattc tggaccaatt ggcgcgtggt gtcgaaattt
     3841 ctcaactctt tatctcaacc tttaccgtca aggctgcaac tgaacttaaa gaacgtttag
     3901 agaaaaaaat cagcaagaaa atccaagaaa cagatgatgt cgatctcaaa caacacttgg
     3961 gtcgccagtt ggcagaccta cccaacgctg ccatcggaac catggactct ttcacacaaa
     4021 aattccttgg caaacatggt tatctgcttg atattgcacc taatttccgt attttacaaa
     4081 accaaagcga gcaacttctt ctcgaaaacg aagtctttca tgaggtcttt gaagcgcatt
     4141 accaaggtaa acagaaagag agctttagtc atttgctgaa aaactttgct gggcgtggca
     4201 aggacgaacg gggtctgcgg cagcaagtct ataaaatcta tgacttcctc caatccacca
     4261 gtaatcctca aaagtggctg agtgaatctt tcctcaaagg ctttgagaaa gctgatttta
     4321 ccagtgaaaa agaaaaactg actgagcaaa tcaaacaagc cctctgggat ttggaaagct
     4381 ttttccgtta ccatctggat aacgatgcca aggagtttgc aaaggctgcc tatttagaaa
     4441 atgttcagtt aattctggat gaaattggct ccctaaatca ggagttcgat agtcaggctt
     4501 atcaggcagt acttgcgcgt gttgtcgcca tctctaagga gaaaaacggt cgtgctctga
     4561 ctaatgccag ccgtaaggct gatttgaagc ccctggctga tgcctacaac gaagagagaa
     4621 agacccagtt tactaaacta ggacaattat cagaccagat agcgattctc gactatcaag
     4681 aacgttatca tggagacact tggaaactag ctaaaacctt ccaatctttc atgagcgatt
     4741 ttgtagaggc ttatcgtcag agaaaacgac aggaaaatgc ctttgaattc gctgatatca
     4801 gccattacac cattgagatt ttagaaaatt tcccacaagt tcgtgagtct tatcaggagc
     4861 gcttccatga agtcatggtc gatgagtatc aggataccaa ccatattcaa gaacggatgc
     4921 tggaattgtt gtctaatggc cacaatcgct ttatggtggg agatatcaag caatccatct
     4981 atcgtttccg tcaggcagac ccgcagattt tcaatgagaa attccaacgc tatgcgcaaa
     5041 atccccaaga aggcaagctc attctcctca aggaaaattt ccgtagtagt tcagaagtgc
     5101 tgtcagcaac caatgatgtc tttgaacgtc tcatggacca agaggtcggc gaaatcaact
     5161 atgataacaa gcaccagctt gtttttgcca ataccaaact gactcccaat ccagacaaca
     5221 aggcagaatt tctcctctac gacaaggacg atacaggtga ggaagaagag agtcaaacag
     5281 aaacgaaact aacaggcgaa atgcgcttag ttatcaagga gattctgaaa cttcatcaag
     5341 aaaaaggtgt tgcctttaag gaaattgtcc ttctgacctc cagccgcagt cgtaatgacc
     5401 agattctcct cgccctgtct gagtacggga ttcctgtcaa aactgacgga gagcaaaaca
     5461 attatctcca atccctagaa gtgcaagtca tgctagacac tcttcgtgtc attcacaatc
     5521 ccctgcaaga ctatgccttg gttgccctta tgaagtctcc aatgtttggt tttgatgagg
     5581 atgagctagc acgtttgtcc cttcagaaag cagaggataa agtccacgaa aatctctatg
     5641 agaaactggt caatgcacaa aaaatggcaa gtagtcaaaa aggcttgatt cacacagctc
     5701 tagctgaaaa actaaagcaa ttcatggata tcctagcttc ttggcgcttg tatgccaaaa
     5761 cccactctct ctatgacttg atttggaaga tttacaacga ccgtttttat tatgactatg
     5821 ttggggcttt gccgaatggt cctgctaggc aggccaatct ctatgcccta gcactgcgtg
     5881 ctgatcaatt tgaaaagagc aatttcaaag gtttgtcgcg ttttattcgt atgattgacc
     5941 aagtcttaga agcccagcac gatttggcaa gcgtggccgt cgcaccgcca aaagatgcag
     6001 tagagctcat gaccatccac aagagtaaag ggctggagtt tccttacgtc tttatcctca
     6061 atatggatca agatttcaac aagcaagact ctatgtcaga agtcattctc agtcgtcaga
     6121 atggtcttgg tgtcaaatat attgccaaga tggagacagg ggcagtagaa gaccactatc
     6181 ctaaaaccat caaactctcc attcctagtc tgacctatag gcagaacgaa gaggaattac
     6241 agctagcaag ctattctgag cagatgcgtt tgctgtatgt tgctatgacg cgggctgaga
     6301 aaaagctcta tcttgtcggc aagggttctc gtgaaaagct ggaagtcaag caatacccag
     6361 cagccaaaaa tgggaaacta aatagcaata ctagactgca agcacggaat ttccaagatt
     6421 ggctttgggc tatcagtaaa gtgtttacta aggacaagct caactttagt tatcgtttta
     6481 ttggcgaaga tcagttgacc agagaagcta tcggagagtt ggaaaccaag agtcctctcc
     6541 aagatagctc ccaagcagac aatcgtcagt cagataccat caaagaagct ctggaaatgc
     6601 tgaaggaggt ggaagtttat aatactcttc accgcgcagc tattgaactt cctagtgttc
     6661 aaaccccaag tcaaatcaag aaattctacg aaccagttat ggatatggaa ggtgtcgaga
     6721 ttgctggtca aggtcagtca gtaggcaaga aaatcagctt cgatttgcca gatttttcaa
     6781 ccaaagaaaa ggtaactgga gctgagattg gtagtgctac tcacgaactc atgcagagaa
     6841 ttgacctcag ccagcaacta acccttgcta gcctaacaga aacactcaaa caagttcaaa
     6901 ctagccaagc tgtcagagac aagatcaatc ttgataaaat tcttgctttc tttgacacag
     6961 tactcggtca ggaaattctt gctaataccg accatctcta tcgcgagcaa cctttctcca
     7021 tgctcaaacg agaccaaaag agtcaggaag actttgttgt ccgtggtatc cttgatggct
     7081 atctgcttta cgaaaacaaa attgttctgt tcgactacaa gacagaccgc tatgatgaac
     7141 caagtcaact cgtagaccgc tatcgtggtc agttagctct atacgaagag gctttatcac
     7201 gagcctattc gattgaaaat attgaaaaat acttgatttt actcggtaaa gacgaggttc
     7261 aagttgtaaa agtataactt atactctttg aaaatctctt caaaccacgt cagcttcgcc
     7321 ttgctgtata tatggttact gacttcgtca gttctatcca caacctcaaa acagtgtttt
     7381 gagctgactt cgtcagttct atctacaacc tcaaaacagt gttttgagct gattgcggct
     7441 agctttctag tttgctcttt gatttttatt gagtattaga aaggagacct catgacactt
     7501 ccagttagaa aatccctgca cgatgcagtt ttacaggctt cgaaagcgga tacttgggaa
     7561 caagctacca aggaatggaa tgaagtttcc ttgattttta acggtatcgg ccgtagcaat
     7621 tgtgtctgtg gaaatgccat caaatacgct tatgaactct ttaacggtgt cacaggccaa
     7681 cgcctctttc caattggtag cgactgtgtt cgtcattttc atcgattgtc ccttgaccag
     7741 caattggaag aggaagaaaa actgctcaga aaggttgaaa atctaaccag aaaagctcag
     7801 aaaaaggaaa aaatcaaggt caataaaagt gactttgacg agcgacttct aaaatggctc
     7861 tgggaaaaag gtgttttcaa acccaatcgt ggcaatcaat ttgcgcctga gagagactac
     7921 cagcttttac tagaagtctt tcagggagga agttggacca aggcggaacc aaagaaaaag
     7981 gctcggatgg aagaagtcct tgaaaagtgt atcaaaccct ttttacttgg gaaatcggat
     8041 gaccaactct accttgtcaa gctaggcaag gagaaaattg actacgaaca agaactccgt
     8101 atccaggcag agaaagaacg caagaagagg gataaaatcg ccaagcaata tgcagacaat
     8161 ctggttctag ccatgggacc cgcagagcgt gcttatcaag attactttgg ctttacagag
     8221 accctgactc aagaagaacg caagtgggaa aaaattcttt ttggtaaaaa tagagcagaa
     8281 cgggctatca aggccaaaca ataccaaaaa gagctggaaa aagaccaacg aattgccagt
     8341 caggatccaa ttgaaagaaa acagaagcag acttggctcc tcaattccta ttttcgtgag
     8401 cttcctgaag aaaaagctcg attttctagg ctcttattag aatatcgaaa aagtggtgaa
     8461 gtaccctttt caactgaata cctgtccgat catctcatcg actttttcta taaaatgaaa
     8521 gcctttgaat ttgaaatcgc accagaacaa gtccgagatt ttctaaaaaa aagcctgcag
     8581 gaagaccatc gctcatcagc acaaggaagc tggatagaag gaattctcct caactgcctc
     8641 aaaccatttt tagaacgatt agtgatatga gtaacaccta ccgtgtaaat ttcatggtag
     8701 gtgttattgt acccccaaat aatataaaac aaagtatgaa agcgcatctc aattttaaga
     8761 cttttttgta aattaagagt ataattaagg tatactg
//
LOCUS       DAVLSL010000084         8567 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLSL010000084 DAVLSL010000000
VERSION     DAVLSL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.085"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            90..542
                     /locus_tag="VXN30_001441"
     CDS             90..542
                     /locus_tag="VXN30_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1358051.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(577..1965)
                     /locus_tag="VXN30_001442"
     CDS             complement(577..1965)
                     /locus_tag="VXN30_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1358052.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2031..2993
                     /locus_tag="VXN30_001443"
     CDS             2031..2993
                     /locus_tag="VXN30_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1358053.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(3199..4209)
                     /gene="ccpA"
                     /locus_tag="VXN30_001444"
     CDS             complement(3199..4209)
                     /gene="ccpA"
                     /locus_tag="VXN30_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1358054.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4634..5233)
                     /locus_tag="VXN30_001445"
     CDS             complement(4634..5233)
                     /locus_tag="VXN30_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1358055.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5235..6332)
                     /locus_tag="VXN30_001446"
     CDS             complement(5235..6332)
                     /locus_tag="VXN30_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1358056.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(6332..7069)
                     /locus_tag="VXN30_001447"
     CDS             complement(6332..7069)
                     /locus_tag="VXN30_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358057.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(7071..7955)
                     /locus_tag="VXN30_001448"
     CDS             complement(7071..7955)
                     /locus_tag="VXN30_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358058.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(7942..8136)
                     /locus_tag="VXN30_001449"
     CDS             complement(7942..8136)
                     /locus_tag="VXN30_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358059.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(8133..8339)
                     /locus_tag="VXN30_001450"
     CDS             complement(8133..8339)
                     /locus_tag="VXN30_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358060.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         2606 a         1973 c         1524 g         2464 t
ORIGIN      
        1 aatattcttt tgaaaaatac ttttttttgc tttacttcta tagtgaaaag gtttacaatt
       61 atagtaagaa aatttcagga ggtttcatta tggcacaacg ttaccaaaat atcatggtcg
      121 caatcgatgg ttctaaggaa gcggacttgg cttttgtcaa gggagttcat tctgctctac
      181 gaaacgacgc taaactcacc atcgcacatg tcattgacac acgcgctctc caaagcgtat
      241 ccacctttga tgctgaagtt tacgaagaac tccaagtcga cgctgaaagt ctgatgaaag
      301 agtacgaaaa acgtgctaaa gatgctgggg tagcagatgt tcatatcgtc attgaaatgg
      361 gaaatccaaa gaccctgcta gcacgtacta ttccagatgc cgaggaagtg gacctcatcc
      421 tcgttggcgc aactggtctc aacgcctttg aacgcctctt agtcggctct tcatctgaat
      481 acatactccg ccatgctaag gtcgatttgc tggttgtgag agaacaagaa aaaaccttat
      541 aatcacaaag aaaaggagcc cctagctcct ttttgtttac gatttatttc tctctttatg
      601 gcgttcgtaa gccttgagct ggcgctgcag ttccttttta atagcaggtt ctggagcata
      661 tttttcttcc caattatctg gttttaagat tttatgggtc actggatcaa aatgagcctt
      721 gccatctgga aaaattttcc ccatattggc ctgatggaca atatcaaaaa tacgttctgg
      781 gtccaccccc atcaagacaa aactgccgta ggtgaagtaa agcgtgtcaa tcaaggcatc
      841 cacttgccct atcaaatctt gctgagcagg tgtcttcttg gctactttac ctgctgcctt
      901 atcaagggcc tgatgaagtt gcgatacagc ttgaccaaaa tcttcttcag aaggactggc
      961 tgatcgaaca aactccacca attcttctat tttaaaacca gccctatggg ttgcaccctc
     1021 taaatcccaa gctcgaggtt cttcttgggt tcgttcatcc atcatgtggt ggaaggtctt
     1081 gaccttattg aaatgatagt cacggctgac aaaaactttt tctgaagaca aaacaccaaa
     1141 atgttccaag gccttgtgga tcccatcttg ttgattgcta gtggtaatgt gcttggcagc
     1201 ttccttgaca ctgctactac catttcccat agcgaccgac ataccgacgc cagccagcat
     1261 ttcaaggtca ttatctgagt caccaaaagc catgacttgg ttgaggtcaa agccatattc
     1321 tttcccaact cggcgaatgc cttctaattt agaatttccc tgattgatga catccgatgc
     1381 aaaaggattg ctacgtgtca atttcaagtc ttcaaaatca gctgccgcct tctcagattc
     1441 ttctggtgtc atcagcatca aaacttggta gataggctga ttcatcaggt gaagcaggtc
     1501 ctcttccttt tggggaacaa ccttgctgac catgcgatta aaagactgac tcaccgtccg
     1561 agttaaaaca gagggaacga agcgactaat tcgttgggaa aaagaaccca gaccaaagga
     1621 catgatttta gaacccaaca tggcatcctt ggtccctaga gcaatctccg tgccctcttt
     1681 tttagcatag ctaattagat ggcgcaaatg taacttggaa atagggctcg tgaacaagac
     1741 tctgtcttta ctaaagatat actggccatt ataggttacc gcaaaatcca gatccaaatc
     1801 gtccatcaat tccttaacaa aaaaaggtcc tcgccctgtc gctacgccaa ctagtacccc
     1861 ttgttctttg acaatcttaa tcgcatcctt agtggatttc aaaacactct tgcgattgtt
     1921 gaccaaggtt ccatcgatat caaaaaaaac agctttgact tccatcctat cccaatctcc
     1981 ccttttgtga tacaatgatt ataccacatt tcagaaagag tgagtaaatc atgcctaaga
     2041 aaatccttgt tttacatacg ggtggaacta tttccatgca ggccgatgct tctggcgctg
     2101 ttgtgacgag ttcagataat cccatgaacc atgtgtccaa cccacttgaa ggaatccaag
     2161 tccacgcctt ggactttttt aaccttccaa gtccccatat caaacccaaa catatgctgg
     2221 tcctctacca gaaaattaaa gaggaagcag ataactacga tggagtggtg atcacacacg
     2281 gaaccgatac tttagaggaa acagcctatt tccttgatac catggaagtt ccccatatgc
     2341 ctatcgttct aacaggagcc atgcgtagct ccaatgagct cggtagtgat ggtgtttata
     2401 attacctaag tgctttacga gtggccagcg atgacagggc tgctgacaaa ggagttttgg
     2461 tcgttatgaa cgatgaaatc cacgctgcca agtatgtcac caaaacacat acgactaatg
     2521 tcagcacctt ccagactcca acacatggcc ccctcggtct catcatgaaa caggaaatcc
     2581 tctacttcaa aacagctgaa cctcgtgttc gctttgacct tgatcacata caaggtttag
     2641 tccctatcat ctcggcttat gctggtatga cagatgagct gattgatatg ctggatttag
     2701 aacacttgga cggtttgatt atccaagcct tcggagctgg taatattccc aaagaaacgg
     2761 ctcaaaaatt agaaagcctt ctgcaaaaag gaatcccagt cgctctggta tcacgatgct
     2821 ttaacggtat tgccgagcct gtttatgcct accagggtgg gggcgtacag ttgcaaaaag
     2881 caggcgtttt ctttgttaaa gaactcaatg cccaaaaagc tcgcttgaaa ctcctcatcg
     2941 ccctcaatgc cggactaaca ggacaggctt tgaaagacta tatggaaggc taatactctt
     3001 cgaaaatctc tgcaaaccac gtcacgtcgc cttaccgtat gtatggttac tgacttcgtc
     3061 agtttcatcc acaacctcaa aacagtgtgt tgcgcagcct ttaaccagcc gcctagtccg
     3121 ctctatggta ttcattaagt caacatctct tgtttaagag cacaaaatca ggaaatcttc
     3181 tcgattccct gattttttct atttacgttt tcgtgttgag ctacgttctg tcaaaccatg
     3241 aggtaagaga acttcacgtt cttccaactc ttccttatgc ataatcttgg tcaacatacg
     3301 catactaatg gcaccaaggt cataaagagg ttgggcaatc gttgtcaagt ttggacgggt
     3361 aaagcgtgag atttgtgaat catcactagt aataatttca aaatcttctg gcacagaaac
     3421 acccttatca gccaaaccgt tcaagactcc tgctgccaac tcatcacctg tcacaactgc
     3481 tgcagttgca tttgatgaaa tcaaacgctc tgctaaggcg taaccatcat catagctata
     3541 tttagattca aataccaaac cctcactata agtgattcct gcttttttca aggtttcctt
     3601 gtagccaact aaacgaacct taccattgat gtcatccact agcggaccgc taacgaaagc
     3661 aatacgctca ttttctttag caaggtaact cactgcatca attgttgctt gcttatagtc
     3721 aatattgaca cttggcaact ggtgctcaac atcgacagtt cctgcgagaa caatcggagt
     3781 acgtgaacgc gaaaattctg agcgaatttt atctgtcaag tgatacccca tatagataat
     3841 gccatctacc tgctttgaaa agagggtatt gacaacagaa acttctttct cgttatcttc
     3901 atcgctatta gctaggacaa tattgtactt gtacatttct gcaatatcat caatcccctt
     3961 agccaaactc gaaaaataac cattggtaat atttggaatc acgacaccga cagtggttgt
     4021 ctttttactt gcaagaccac gcgcaactgc atttggacga taatccaaac gatcaattac
     4081 ctctagcact tttttacggg tattctcttt tacattttta ttgccattga ccacacggct
     4141 gaccgtcgcc atggaaacac ctgcttcacg agcgacatca taaatggtta ctgtatcatc
     4201 tgcattcatt ccttttcctg tcctttctat ctcacacatt cttttacaag tagaggtact
     4261 gattgaagct ctatatctac ttacaaaagt gaagatgtga aaatttcgtt ttcatatttc
     4321 tacttattcc attctatcac taattgtaaa cactttcaag tgttttttga agattgattg
     4381 aaaaaatttc atagaaaacc taggtttagc tccttgctac caccttagac taaacaaaaa
     4441 ggaggaaact aagccctcct aaagttatag taaaatgaaa taagaacagg ataaatcgat
     4501 caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt
     4561 caatctacta taggtattgt tccattcact accgtcaatt ttagcacata gtcttcatga
     4621 aaatattata tcatcataac caaccagatt cttttgcgat attagctgcc tctgttcgat
     4681 taccagcatc cagttttgaa agaatattgg tgacatagtt tcgaatagtt ccgtttgaga
     4741 ggtaaagctg atctgcgatt tcttggttgg ataagccccg ggcgattccc tttaagacag
     4801 caatctcttg ttctgtcaac ggattgggac gggtcatcac catttccatc aattcaggcg
     4861 aatactcctt gcgtccttcg aggacggtgt gcaaggtttg catgaggtct gcaatgcttc
     4921 gttcctttaa tacataagca tccactccag ccttgaccgc acgttcaaaa tacccagcac
     4981 gcttgaaggt cgtcaccaca accacctttg tttcaagctt ttctgatcgt atccactcca
     5041 agacttcaag acctgtctta acaggcattt ctacgtcaag gatggcgata tctacagact
     5101 ccttttctaa tagttggatt gcttcttgcc cattcttggc ttgaaggaca gactccacat
     5161 ctggttgaag cgtgagcaat tgacacatgg catctcgcaa catactttga tcttctgcga
     5221 ctaatacttt catcttagtt tctctcctta taaggtagtc gaacttgcac ttccgttgga
     5281 tgtttctgac tgattacact tacttctcct gaaaatggaa aaacacgatc tcggactgta
     5341 tggagctcat ctccttttag aaaagcaaag ccgcagccat catcacttac tgttaaaatg
     5401 agttccttct ctgtccgttc taattttaag taagctttag acgctttggc atgtttgatg
     5461 atattggtca ccaactcaag caaaatcata gaagccattg actccaattc ttgagtaagg
     5521 ctagcagtat ctagttggtt atccgtttcc acctcaattc cagcaatttc taacatcttt
     5581 ttcacagtct ctagttcgga tgtcaaagtt ctagacttaa gattttccac gatagttcga
     5641 acttcattca tggaatcctt gctgatctgg tgaatttctt ttaattcctt ttccacctgt
     5701 ggataagcct ccatctgaaa taactgcaag gctagatcag ttttaacact aagcatagca
     5761 aaagtatgcc ccagactatc atgcaaatct tgaccgatac gattgcgttc attttcagca
     5821 agcaatagat ttatctgggc attttgctta gcctgtgctt ctttcaaatc ctccacaatt
     5881 cgaatccgaa ccaagccaaa agtcattaaa tcgacaaaag caagaattac aagtagatag
     5941 aatagaaact caacttcgat tctctgaaaa atcaacagtt gacccacaac aaggacttga
     6001 gcaagaagaa aagtccagac atgtaaagac tttaaaccac ctacgctgaa atgatagctt
     6061 aagagattgg ataggaaaaa gaaaaaccag atataattaa cagccacaaa ggcagtattc
     6121 ccaactacat aagtcagcag gatgccccaa tatagccaag atagacgctg gctcttagtt
     6181 gttaaaacac ccaaatacgc cactacaaat agaatatcaa tcaataaatg ccaggcagaa
     6241 agccacccag tcactacagt taggatgggg aaaaccataa aaattaaact gatccaaaac
     6301 atataatgta tgcttttcag tctttcaagc attaagcatt ctccttatga ccttgaaggt
     6361 aaatggtcaa accaaacaaa actactgaaa aaacaagtaa ataaactgtg gctgatagat
     6421 tgatgccacc ctcatttaag aaggtcttga gcaactccat caactgatag gtcgggagac
     6481 acttacctac tacttgcatc cagtctggaa ataaagagat aggcatccag agtccaccta
     6541 aaacagccaa ccctagataa agaagattgc ccacgacaga catcaactga ctagttggta
     6601 agagagtcaa ggtcaaacca agcgctacga aggcaatact tcctactatc agcaaaagtg
     6661 cagccccaat ccaatttcca agagacatgt ccacacctct tacaaaatgc ccaactgaga
     6721 aaaccaccaa gattgaaacc aaataatcaa ccagcatact tgttatcttt gatagataat
     6781 attctaccat atttacaggg ctatgacgca atgttttctg ccagttgttg atcttgtcgg
     6841 tatgtaaaac aactgggaat gagaagatag ctgttgacat catggaaaat gcagtcatgg
     6901 agataagata atcacgcata aaattcgcga gttcacctgg tgtgtcctga tagataccag
     6961 aaaaaaataa atagaaagcc gtcggcatcc ctaccgacaa tagataatag attaattgtc
     7021 gtttggtcaa taaaaattct atcttactaa gtgctatcca tcgtttcatc ttagttatct
     7081 cccttctgcg tctcttcaaa gattgtattc aacaaactac gattattaac ttcgatttct
     7141 tgcatcctac atcctgcttg ggctaatagt tcccagaaag catctgcttc tcgagtgact
     7201 acttgtaaag aatcttgctt tagggtccag ttttcaacca aatttgactg ctctacgact
     7261 tccttgtaag ctataggaag gataaagtgc ttttctattt cctcgctacg catagctaga
     7321 ggcgtcgtat ctcgaatcaa ctctccctta tttaagagca aaatgcggtc tgcagtgtgt
     7381 tccacttcct caatataatg ggacgaatag agaatggtga ctccttgcgc ttttaactcc
     7441 tggacaattt cccaaaaacg ttgacgagtt gaggtatcca tggacgcagt aggctcatct
     7501 aaaaaaacaa tctttggtcg cccaattaag gtcaagatga aagaaaagag acgcttttga
     7561 ccgcctgata atttttctgc caactgttct ttttgttgct tgacaaactg caatagttga
     7621 tcaatttcct gattgctcaa aggatttgga tagatacgtt gaaagaaagc aatcaactct
     7681 ttaactttta atttctgaac gattatattt tcttgaggga ggtaagccct agtatagtct
     7741 aacttagaac tcgtcactgg taaaccttga atagatactt gaccgcttgt gaccagttta
     7801 tctccaagca aacaatctaa gagtgttgtc tttccggcac catttggacc aattaaggca
     7861 acacattcac catcgttgat ttcaaaggag atgttccgca aaatttcctt gtcttttatt
     7921 ttcttactca atttttcaac tttaaccaca gtcatgagac tctcctttca accactccat
     7981 tcccataggg aaaacgacga aaatcataaa tccaaacccc aaagcaccac aaatgaattg
     8041 gcgaagcaag gcttgatcaa accaatcagt aaacatttcc actaaccata ccaagagtga
     8101 caggccgata aagaaataga tgatccctct cttcattcct caaactcctt tttcacatct
     8161 ctgactaatt tcaaaccttc tctaacaagc caagacatga ttccaaagcc agcaaagacc
     8221 ttccaaggaa aatggtagaa atcctcatcc aatcccgaaa acatgagata ggtcataact
     8281 cctgctacta ctaaactcat tgcaataatc attttatttc tcatctcttc ttcccccatt
     8341 tcatactaca attatagtct tttaaaatca gaggaggcag aagcttctgt cactagaaaa
     8401 tatgacaaat gtcataaaaa ttctgttcaa aacaagcaag atacactata caataaaaca
     8461 caatcagaaa aatctaaggc aacttcctca aaagagatat caaacccaat tcacaccata
     8521 atgtaaacta atacttattt aaaatcaaaa agagtagaaa tttttat
//
LOCUS       DAVLSL010000085         8562 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLSL010000085 DAVLSL010000000
VERSION     DAVLSL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.086"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(43..675)
                     /locus_tag="VXN30_001451"
     CDS             complement(43..675)
                     /locus_tag="VXN30_001451"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1358061.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(709..1410)
                     /gene="pyrF"
                     /locus_tag="VXN30_001452"
     CDS             complement(709..1410)
                     /gene="pyrF"
                     /locus_tag="VXN30_001452"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1358062.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(1643..2851)
                     /gene="divIB"
                     /locus_tag="VXN30_001453"
     CDS             complement(1643..2851)
                     /gene="divIB"
                     /locus_tag="VXN30_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1358063.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEKEE
                     PESKEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYAT
                     MKDIRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQF
                     PTKFTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIK
                     VFVSELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSK
                     IKPQLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNR
                     NQTTQRSSRR"
     gene            complement(2861..3919)
                     /locus_tag="VXN30_001454"
     CDS             complement(2861..3919)
                     /locus_tag="VXN30_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1358064.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(3923..5275)
                     /locus_tag="VXN30_001455"
     CDS             complement(3923..5275)
                     /locus_tag="VXN30_001455"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1358065.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(5379..6020)
                     /locus_tag="VXN30_001456"
     CDS             complement(5379..6020)
                     /locus_tag="VXN30_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358066.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(6022..8043)
                     /locus_tag="VXN30_001457"
                     /pseudo
     CDS             complement(6022..8043)
                     /locus_tag="VXN30_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(8126..8425)
                     /locus_tag="VXN30_001458"
                     /pseudo
     CDS             complement(8126..8425)
                     /locus_tag="VXN30_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         2451 a         1927 c         1555 g         2629 t
ORIGIN      
        1 taaaagagac tcggtagcct cgtctctctt tatcttactg acctaacctt cttgccaatt
       61 ttcttggtct tctttaaagc gttttagaag atcaaggccc tctggcgtga tgtaaccttc
      121 ttcttgggct agatggataa gctcgctata gtttgaaagc gtcacaagtt taacaccagc
      181 atctgcaaag ttcttatctg cttttggcaa ttggtagctg aaaatcgcta caactccaag
      241 tacatctgct ccttctcgct tggctgctgc tacagcttca agaactgaac cacccgttga
      301 aataaggtct tcaaccacta ccattttttg accttgagct acgcgacctt cgatttgatt
      361 accagctccg tggtcttttg gtttgctacg gatgtaggca aaaggcaagt ccatcttatc
      421 agcaataatg gctccgtgtg gaatccctgc tgttgcagtt cctgcaatca cttctacttc
      481 aggaaaggct tctttgatag cttccacaaa accattttca attagggtac gagtttctgg
      541 ataggctagt gtcacacgat tatcagtgta aatcggtgac ttgataccag atgcccaagt
      601 gaagggttcc tctggtttga ggtaaacggc ttggattttc aagaggtggc tagcgatatc
      661 tttagcaagt gtcatggtat tctcctttta tttttctaat ctagttcttt aattccagtc
      721 ctgtgtccat tcatccttga tggcatgata agctgcaaca ggatcctcag cttgggtaat
      781 gggacgtccc actacgatat agtcactgcc gatttgatag gcatcagcag gtgtcatgac
      841 tcgtttttga tctccaactg caacaccagc tggacgaatc cctggtgtca gacagataaa
      901 atctggattg gtagcctgct tgatgacttg tacttcctga gccgagcaaa caacaccatc
      961 caagccagct tcagctgtct tcttggcata gtgaatcaca gactcttgca gactggtttg
     1021 gatattttga aactcctgca tctgggcttc tgacgttgat gtcagctgcg ttacagcgat
     1081 caatttagct tgactcccaa ggccttcacg cgccgccttc atcatctcta caccaccagc
     1141 cgcatggaca ttagtcatat cgacaccaag ctgagacaag accttcatgg ctgacttgac
     1201 tgtattagga atgtcatgaa gtttgagatc caaaaagaca ctatgaccca aaccttttaa
     1261 gtaggacaca atctcaggcc ccgctgcgta ataaagctcc atccctacct tgagataaag
     1321 gctttcttct gctgggaaaa gagctaaaaa ttccttgacc gcctcaaaac taggaaaatc
     1381 aagagcaatg actggacgat gttctcgcat aggtttctcc ttttaccttt actagtgaga
     1441 gagtctggcc aacagaagcc acgaaaaaag gaacctgcca acagcaaggt tccacgaaaa
     1501 atgggtagtc tccacccttt caccgtctaa ccttagctgc ctctctggac tgctttaaag
     1561 gttttttact attctattat ttctactagc acttgtcaag taaaaacatg gtcactaaag
     1621 aactataaga gaaaaggtaa acctagcgac gcgatgagcg ctgggtcgtt tggtttcgat
     1681 tgctctcttc ctcttgtttt ttctgttctt cttcttgttt tttctcagct tccttagcct
     1741 cttgtttggc tttttcctca gcttccataa ttaatttatc cgccacagtg taactgtaaa
     1801 ttccagcttc catgtcaatc acactcggtt ctgacaattg tggcttaatc ttactataat
     1861 aaggcagttt cttgctcatt tcagaaagcg gaaccaagac ttcgtccaaa tcattcatag
     1921 tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt tggatagccg
     1981 ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt tgttcactat
     2041 cattaaagag aactgataaa taagtttctg gtaaactgtt caggctcaca gaactagtct
     2101 caagctgacc actggaaaga ataggataat gattttcacc agaaatatag taggccacaa
     2161 tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca agttgagctg
     2221 attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct agcagaaggt
     2281 taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca gctgtagttt
     2341 gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc aagtaggcag
     2401 agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag atatggatac
     2461 caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagcaagc tttttatcct
     2521 gttcctcctt ctctttagac tctggttctt ctttctcttc tttctcttct ttctctttgt
     2581 catcctctga ggatgctact ttttcttcag actcttcctt agctgattct gaatcttcct
     2641 ggtctgtttc actctcctgg tcctgtttgt cctctgactt ctcagattct tctcccatcc
     2701 gagcttgtct ttccttttcc ttctcctcag ctagagccgc ttcttcttca gccttctttt
     2761 ttagatattc ttggtttcgt ttctgccatt ctgataactc tttcaattct tcgaggattt
     2821 ctttgtcctc atttttctta tcttttgaca tttactttcc ttatgataaa tcttttttca
     2881 acaattgata aaaatctgct agagatttca attccttaga agctttcatc ttagcttggt
     2941 aatcttcctt gtgacttagt aagtgagaaa gcttctcttc caaactatcc aaggtcaaat
     3001 cgctttcttg aaggtcttct gcatagcctt tttttacaaa gtaagctgca ttttcaatct
     3061 ggtcaccacg actagcttca cgaccaagcg gcacaatgac atgcaatttt gctatcgcca
     3121 agagctcaaa aatcgtattg gcaccacctc gtgtcacaac aatatcagcc aattccaaca
     3181 agggttgata gagatcggtc acatagtcaa cacgaaaaag attttggctc aactcattca
     3241 ggctagaatc tccagttaga ttgataatat tgtagcgctc tgttagttct ttcttatggt
     3301 ctgtcaccaa ttggttaaag acacgagcac ctgcagaacc gccaacaaac aatacagtcg
     3361 gcaatttatg attaaagtgg gtttgaatat ccaccaattc atctggttct ggattttttt
     3421 gatctgaaac cttggtcact gctcccacat gctcaacctt agacaaactc gaagcttgct
     3481 caaaggttga atacatctta gtcgcaaatt tataggcgat tttattggcc aagcccatag
     3541 acaggtcaga ttcgtgaata aagacaggca ctcctgacac acgcgctgcg ataacaggcg
     3601 gtactgagac aaagcccccc tttgaaaaaa gggtctgtgg acgaagtcgc aacatgataa
     3661 agagcgattg gacgattccc cagccaactt tgaagacgtc cagcatattt tgccaagaga
     3721 aatagcgacg caatttccca gtcgcaatgg agtggaaagt gacatccaaa cctgacttaa
     3781 ggatttcttg gtgttcgata ccacgcttgt ccccgatata gtggacttcc caaccatctt
     3841 cgatgaactt gggcattaac aaaagattga gggtaacgtg tccaaccgtc cccccacctg
     3901 taaagacaat ttttttcata ttttattctt ttaactccgc tactgtgtcg ataaagaggt
     3961 cgccacgtac ttcaaagtta gcatacatat cccagctggc attggcagga ctaagaagaa
     4021 ccacatctcc ttgagtcgca agctcatagg ccttgcgggt cgcatctgca atatctgtcg
     4081 cctccacata agcgacacca gccttgtctg ctgcccgttt gacacgttct gcagattgac
     4141 ccaggatgac catcttcttg agtccagtaa tgtctggcac caattcgtca aactcattgc
     4201 cacggtccaa accacctgca atcaagacga ccttgctgtt gtcaaatcct gataaggctt
     4261 tttgagtagc caagatatta gttgatttac tgtcgttata gaatttaaca cccttgatgt
     4321 catccacaaa ctggagacgg tgtttgacac caccgaaggc tgaaagagtt tccttgatgg
     4381 tttgattgtc cacatcacga agcttggcta cagcaatagt cgcaagggca ttttccacat
     4441 tgtggctacc tggaacaccg atttcattcg ctgccatgac tacttcacca cggaagtaga
     4501 gttgaccatc ttccagataa gctccatcaa ccttttcaag tgttgaaaat ggtacaacag
     4561 tggcttctgt cttggaagcc aagtctttta ccaagtcttg attaaagttc aagacaagga
     4621 aatcagctgc tgtcatcttg ttctggatat tccacttggc tgctacatat tcctcaaata
     4681 atccatgata atcgatatga gttggcatga ggttggtaat aaccgcaatc tctggatgga
     4741 attcttgaac acccatgagt tggaaagaag aaagttccat aacaagcgtg tccttatctg
     4801 atgctatttg agcaacctga ctggcaggat agccgatatt ccctgataaa agaccatgtt
     4861 gcccagcagc agtcaaaact tccccaatca tagtcgttgt ggttgtctta ccgttcgatc
     4921 ctgtgatacc aataatcggt gcttctgaaa tcaaataagc caattccacc tcagtcaaga
     4981 ctggaattcc cttggccaaa gccttttcaa tcatgggatt gctgtagggg atacctggat
     5041 tttttaccat gagggcaaac tcttcatcca agagttccaa aggatggcca cctgtaatga
     5101 ccttgatccc ttcttccagc aaacattggg cagctggatt gtcctcgaat ggtttcccat
     5161 catttactgt cacaatggca cctaacttgt ccaacaaacg agccgcagat tcaccagact
     5221 tggccaaacc taaaacaaga actttcttat ttttaaattg atctattact ttcatgtctc
     5281 gaactccatt tctactccta ctattttacc atttttatgg aaataaaaaa gccacaaagt
     5341 gtgtttgtga ctctttcttc taactgaatc ttaccatatc atctatgtga taaatcggta
     5401 actcgaatga cctgatccac ttgctcccaa atcagaggat tgtgggtcgc aataataatg
     5461 gtccgattcg gattttttaa agattctagg atggaaagta attcctcaga atttttgggg
     5521 tctaaggaag cggttggttc atctgcaaga atcaaaggcg gatcctttaa aattatcttc
     5581 gctagtgcaa cacgttgtgc ttctcctcct gataactcaa atataggttg cttcaaatcc
     5641 aaataagaga ggtttacaca gtttagagct tgtttcatca aagagatttt ctctttttcc
     5701 ttcaactttt taccaactaa acccagattg agattctctt tgacggtttg gctttcaatt
     5761 aagccaaaat cttgaaataa gtatcctaag taatctctaa agaaaacaga aggcttgatg
     5821 tccttaagag aagtgccatc atagatgatt tgccctttgt catatggctc caatcgtcca
     5881 atcatattca agagtgttgt cttaccacag ccacttgtac cgattaaggc ataaattttc
     5941 ccaccttcaa aatgaagatt cgtatctgaa aatagctgac ggcttccaaa ttttttagat
     6001 atattcttta gttcaatcat cctattttcc tttcataatt gtcatagaaa cacgagattc
     6061 tttctgcgct tgacggtaaa gcgtcaaaac tgcactagct agaaagacca ataaagtgag
     6121 caagccaatc accaagtctc gactgcttaa aataaagaga ctagcaccaa atacaaaact
     6181 ggcaaattgg ctaaccatat actgagcatg tgtttcaaaa aatcgtaaac ctgaaattcg
     6241 tttaatcaag atatctcggc ggaattgctc gaaatataga agattgacag aataaaatag
     6301 taataaggaa ctagctatcc ccacaatagc tcctaagatt aaagttgctg tttcagtttg
     6361 aacttcatta taacgagtta gataaacact tcttccttct ttaagatagg atacttgctc
     6421 ataaattcca gctttcttca agagttctag cccactctca tatcctttga taaagagttg
     6481 ttttccagca ttgatagacc aactagataa ggatataaaa ctatcacctg tagaagtcgg
     6541 cgtgaatacc actaaaatcg gatcagtcaa atactgagta gatacgggat tctcaccgtt
     6601 attataaaca aaccgctttt ctcccattga aagataacta acgtgcgctt tcatctcata
     6661 atccaaagga gcacttgcct cctcaccaga ttttccataa taactcaatc tttcttcaaa
     6721 aactttctta agttctgctt ctcgagagcg caaatgttct gggagcaaga ggataaactc
     6781 acctttttgg agatgggcta acttctgttt ggtctcagca tctaccacga ccttttcctt
     6841 gtccaaataa ctgggactaa catagagcgt attagcatct ggactatagg tatccagtgt
     6901 ctctccctgt tcattttttc cttgtggatt ggcaaaatgg agcagattat cctttacata
     6961 aagagcttgt tcctcttcga ttgcttcctt ggcaaaggca taccacttgc tctgattttc
     7021 tgtatctttt cctctatcac ctaagccaaa agaaatttga taatagtctg ctctatcctg
     7081 ccatgcttgt tttgaaattt caagttcttt caatcgttgg taagacgtca aacctgtctt
     7141 aacagcgtat cctactgtaa agacagcaac caactgacac aatagggtta aaatcatcag
     7201 gcgtttaagg ggtaatctcc ccttaataac gggaactaat gctttgtaac tcaaactcat
     7261 tagataaaga agcattagta aaattgaaat cgccaataaa aacaacagat agaaactaat
     7321 cccaaaacca taggtggcta acaagatagg ataaaacaaa ccttgactaa aaagaacgac
     7381 tcccccacct aggaaggaaa ggagggctga tagaaggagc tatttgatat cagtagataa
     7441 agaatgcccc atgatggata agagagtctg accagaaaag agttttatac ctgctgctct
     7501 catttcctta atccgagtga taatcactaa agcaaagaaa gataagccaa atattgctaa
     7561 actaattaaa ataaggggat ttagtaatat tcgaaaagca agaaaatagg gcggtatctt
     7621 tcggtcagca cttgctttat aacccaaatc tcctaattta tcggcaagct tttctttcgt
     7681 caaggagcct gacaaaagga gataactatt tagcggatca ctacgttcac gactttcttg
     7741 gctagcttct tggaattctt ttggtaaagt tccctgacca taagttgcat aagtaaagtg
     7801 agtcgtccca tccttacttg gctctacaat tcttctagct attaaactct gttctgagtt
     7861 tgcaaaattc tccaattcct gttcaaatac ctcacgcgtc ggttcctgag tatctttttt
     7921 gacacgaagt aaagaaacgg aatcatagct tgcatataaa tattgtggcg cacgtaagac
     7981 aataatccaa gcaaggaaga agctgagaaa aaaagttgat aataatatga atagtttctt
     8041 catagtagac tccttggtag actccttgta aacaaaattc cccctgtaat ttcttacaag
     8101 gggaacgatt taaatcaatg aacgattagt cataatcaca gtaaaatgct acttgttctc
     8161 cccatttagt ccaaatccat gcataggagg tttttccagc aggagcttct cctttatcag
     8221 aatgaccatc ccatctgctc acgatggatg aataatgata ttttttacca tgatagtaat
     8281 ttgaaaaagc ctaaccacct cctgaacctt ctccatatgt ccatactcct ccatctggat
     8341 attatacagc agctgatgca gctcccaata atgtaaaact tgaaataaga gctagagcaa
     8401 gtaatctatg ttttttcgtt ttcattttat tttttctttc aaaaaaatca caccttgagc
     8461 aacaatgcaa caaaataaat cctcctctct cttttattga aaccgctttc ttatgtgata
     8521 agaataacat ttttattatt tgttgtcaag gaaaaaatcg aa
//
LOCUS       DAVLSL010000086         8383 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLSL010000086 DAVLSL010000000
VERSION     DAVLSL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.087"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..212
                     /locus_tag="VXN30_001459"
     CDS             <1..212
                     /locus_tag="VXN30_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1358067.1"
                     /translation="FARVLLGVLKVTQHNSRKTWYYVPLQDFTVNSDIDWTQSVTDID
                     RQLDQKYDLSPEEIAFIENHVREMD"
     gene            377..3631
                     /locus_tag="VXN30_001460"
     CDS             377..3631
                     /locus_tag="VXN30_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1358068.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
     gene            complement(4017..5003)
                     /locus_tag="VXN30_001461"
     CDS             complement(4017..5003)
                     /locus_tag="VXN30_001461"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1358069.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            5197..7665
                     /gene="gyrA"
                     /locus_tag="VXN30_001462"
     CDS             5197..7665
                     /gene="gyrA"
                     /locus_tag="VXN30_001462"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1358070.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            7665..>8383
                     /gene="srtA"
                     /locus_tag="VXN30_001463"
     CDS             7665..>8383
                     /gene="srtA"
                     /locus_tag="VXN30_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1358071.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAF"
BASE COUNT         2697 a         1446 c         1808 g         2432 t
ORIGIN      
        1 aattcgctag agtattatta ggtgttttga aagttactca gcataattca cgcaaaactt
       61 ggtattacgt ccccctccaa gactttacgg tcaattcgga cattgattgg acacaatcag
      121 tgactgatat tgaccgccag cttgatcaaa aatatgacct ttcccctgaa gaaattgcct
      181 ttattgagaa tcatgtaagg gagatggatt agaaaagtat ttttatttga caaatagtgc
      241 tcaatgatct aaaatgacta tataggatta ggtcaggaag catacgatgc cctgaccctt
      301 tttgtactta tgagatgaga aagtcatttg ttagataaat tgacttcgtt agcaaacgtt
      361 caaaaaagga aaacttatgc cagtagaaat taaaaccact aaagaaattc atcctaaaat
      421 ctatgcctac accacaccga cagtaaccag taatgaaggc tggattaaga ttgggtatac
      481 agaacgtgat gtcacacaac gtatcaagga gcaaacgcat acagctcata tagctacaga
      541 tgtcttatgg actggtgatg cagcttatac agaagagcct gataagggga aaactttcaa
      601 ggaccatgat ttccaccatt tcctttcttt ccatgatgta gaacgtcgtc ccaagacgga
      661 atggttctat tttaatggaa ctcctgaaaa atcaaaaaat ctttttgata agtttgttca
      721 gcatgatttg tctggttatc agcctggaaa aggacaggac tatactctgc gacaagagca
      781 agaagaagca gttgctaaga cattagctta tttccaagaa catgctggag gcaagtttct
      841 ctggaatgcc aagccacgct ttggtaaaac cttgtctacc tatgacctag ctcgacggat
      901 ggaagctgtc aatgtcctaa ttgtaacaaa ccgccctgcc attgctaact catggtatga
      961 tgattttgaa acattcatag caggtcaaac gacttacaag tttgtttctg aatcagatag
     1021 ccttaagagt cgtccaatct tgtcacgaca agaatttctt ggtattttag ctgacgatgt
     1081 aagacaactt gcttttatca gtctccaaga cttgaaagga tctgtttatt taggtggaga
     1141 gcacgataaa ctcaaatggg taactgatct gcattgggac ttgttggtta ttgacgaggc
     1201 tcatgaagga gttgatacct tcaagactga ccaagccttt aataagattc gacgaaattt
     1261 tactctgcat ttgtcaggta catcatttaa agcattggct aaaggagatt ttacagagga
     1321 acaaatctac aactggtctt atgctgatga gcaggctgct aagtattcgt ggtctcttga
     1381 gcaagaagag gaaaatcctt atgaaagctt gcctcagttg aatctcttta cctatcaaat
     1441 gtctcagatg attggcgaaa agttagaaaa aggcgctcag atcgatggtg aaaatattga
     1501 ctatgttttt gacttaagtg aatttttcgc tacagatgat aaagggaaat ttattcatga
     1561 gcatgatgtc agaaattggt tagatactct atcaagcaat gaaaaatatc cattttcaac
     1621 caaagaactc cgtaatgaac tcaagcatac tttttggctt ttagaacgtg tcgcttcggc
     1681 caaagcatta aaagccctac tagaagaaca cccaatctat gaaaactatg agatcgttct
     1741 agctgctggt gacggacgta tgtccgaaga agacgataaa gtcaaactca aatccttgga
     1801 cttggttaga aaagcgatag cagagaatga caaaaccatt accctatccg ttggtcagct
     1861 gacgacaggt gtcactatcc ctgaatggac aggtgtattg atgttatcaa atttgaaatc
     1921 accagctctt tatatgcagg ccgccttccg tgctcaaaat ccttactcat ggagcgataa
     1981 caaaggaaat cactttcgca aagaaagagc ctatgtattt gactttgcgc cggaaagaac
     2041 cttgattctc tttgatgagt ttgccaacaa cttatcgctt gtaactgcag ctggtagagg
     2101 aacttcagct acacgcgaag aaaatattag agaattatta aacttctttc caattattgc
     2161 cgaagaccgt gctggtaaga tggttgaaat tgatgcaaag gcagttctaa ccactcctcg
     2221 ccagataaaa gctagagaag ttcttaaacg aggttttatg tccaatctct tatttgataa
     2281 tattagtggt attttccaag caagtcaaac agttttagat attttaaatg agctgccagt
     2341 tgaaaaggaa gggaaggtac aagatagttc tgatttatta gatttttcag atgttacagt
     2401 cgatgatgag ggaaatgcag tagtagacca tgaaattgta gttaatcagc aaatgcgact
     2461 ttttggtgaa aaagtttatg gacttggtga atctgttgct gagttagtca caaaagatga
     2521 ggaacgaact caaaaacagc tggtcaatga cttgagtaag accgtttctt cagtgattgt
     2581 agaggaattg aaagcagatt attctctaaa aacaagggaa actgagcaaa ttaagaaaca
     2641 aattacagca acacttgaga atgaaattcg aaaaaatgat atcgaaagaa aaatttctga
     2701 agctcatatc aagcaagagt tgcaacagca gctcaaagaa gcaaatgata aagcgcaaaa
     2761 agataagatt caagaagatt tggaaaaacg tttagaagaa aataaactca ttcataaaga
     2821 aaaactagaa caaacactca aaaaagaagt ggaaaaaatg cctgagaaat ttatcgaaca
     2881 ggttgagata aaacgtgtgg aacagttgaa acaatcagct caagatgaaa ttcgtgacca
     2941 tttacgaggg tttgcaagaa caattccaag ttttattatg gcttacggtg atcaaactct
     3001 aacacttgat aattttgatg cctttgttcc tgaacatgtt ttttatgaag taacagggat
     3061 tacgattgat cagtttagat atttgcgaga tggtgggcag gattttgcag ggcatctctt
     3121 tgataaagca acatttgacg aagctattca agaatttctt cgcaagaaaa aggagttggc
     3181 ggattatttt aaagatcaaa aagaagacat ttttgactat attccaccgc agaagaccaa
     3241 ccaaattttc actcctaaac gagtggtgaa aaggatggta gatgatttgg aaaaggaaaa
     3301 tccagggatt tttgatgatc catctaagac ttttattgat ttatatatga agtcaggcct
     3361 ctatattgca gaacttgtga agcggttata taatagcaat ggcttgaaag aggcctttcc
     3421 aaatcctgaa gaacgcttaa aacatatttt ggaaaagcaa gtttatggat ttgctccgtc
     3481 tgagattatc tataacattt ccactaattt tatatttggt aatctttcta aagatatcag
     3541 taggaagaat tttgttttag cagataccat tccagcggct aaagaaggga gcattcaaaa
     3601 gttggttgat tcctattttg aaaataatta aaaagaaggc cgagtcaaaa ttctttgaaa
     3661 tcagaaaaaa cgcataatat tgagtgcttt tgtactgccc cccaaaagtt agacagaaaa
     3721 aatctaactt ttggggggca gttcagacaa tccttggtat tatgcgtttt attgtgggaa
     3781 gatgtataat ggattgaaat aagatatgaa caaatcgatt aggaaaggaa aattgattta
     3841 tagaaatgtt ttagtagtcg atgcgtactg ttatagattc aacgaactat aaaatctgaa
     3901 gaaagcaatt ttaaccagat cttagaagca gttgtgtact actctaactt caatagacta
     3961 tatcatatag ataaaaaaca actccctgat gattcaagga gttgtctata gttaaattag
     4021 tttttagaag cttcttggaa ttctggattt ttccatgctt cgtcaataat agcttgcaat
     4081 tctttagcag atgcttgcat tttttgagtt tctgcatcgt tcaatggaat gtttactgga
     4141 cgaacgatac catgtgcacc aacaacagct ggttgaccga taaagacatt ctcaactccg
     4201 tattgacctt cttggaatac tgaaagtgga agtactgcgt tttcatcgtc aaggattgct
     4261 ttagtgatac gagcaagggc tactgcgata ccgtagtatg ttgcaccttt tttgttgatg
     4321 attgtgtagg ctgcatcacg aacaccttcg aacaattcaa tcaattcagc ttcttgaaca
     4381 ttttgagtgt ctttaaggaa ttcttcaagg tttacaccag cgatgttagc gtgtgaccaa
     4441 acagcgaact cagagtcacc gtgttcaccc atgatgtagg cgtgcactga acgagcatcc
     4501 acatccaatt tttcagcaag tgcttgacgg aaacgagctg agtcaagtga agtacctgaa
     4561 ccgataacgc gttctttagg gaaaccagag aatttccaag ttgagtaagt caaaacgtca
     4621 actggattag cagcaacaag gaagatacct ttgaaaccag attcaacaac ttgagttacg
     4681 attgatttgt tgatagcaag gtttttacct acaaggtcaa gacgagtttc acctggtttt
     4741 tgaggtgcac ctgcagtgat cacaacaagg tcagcgtctg cacagtcaga gtattgagct
     4801 gcatagattt ttttaggtga agtgaaggca agggcgtgac taaggtcaag cgcatcacca
     4861 acagcttttt catgcaattg tggaatttcg ataattccaa gctcttgtgc aattccttgg
     4921 ttaacaagtg caaaagcgta agatgaacct acagcaccat caccgacaag gataactttt
     4981 ttgtgttgtt tagttgaagt cattgtttta aacatctcct taattttatt aggggatttt
     5041 ccctagacaa cttcattcta tcacttttaa aaaactttgt cacgaatatg ccttatagtt
     5101 ctcgatgtaa acgttttagt ggtttagagg ctgaaataga tgggaattta tggtataatg
     5161 ttgttactta ctaattgtga aatgaggcat ttattaatgc aggataaaaa tttagtgaat
     5221 gtcaatctga caaaggagat gaaggcaagt tttatcgact acgccatgag tgttatcgta
     5281 gcgcgagctc ttcctgatgt tcgagatggc ttaaaacctg ttcaccgtcg cattctctac
     5341 ggaatgaatg aattgggtgt gaccccagac aaaccccata aaaaatctgc tcgtattaca
     5401 ggggatgtca tgggtaaata tcacccacac ggggattcct ctatttatga agccatggtc
     5461 cgtatggctc aatggtggag ctaccgttac atgcttgtag atggtcatgg gaattttggt
     5521 tccatggatg gagatagtgc tgccgctcaa cgttataccg aggcacgtat gagcaagatt
     5581 gctctggaaa tgcttcgtga tatcaacaaa aatacagttg atttcgttga taactatgat
     5641 gccaatgaac gggaaccctt ggtcttgcca gcgcgttttc caaacctttt ggttaatgga
     5701 gcaactggta tcgcggttgg gatggcaacc aatattccac ctcataatct gggtgaaacc
     5761 attgatgcag tgaagttggt catggataat cctgaagtga ctaccaagga cttgatggaa
     5821 gtcttgcctg gaccagattt tccaactggt gctcttgtca tggggaaatc aggtatccat
     5881 aaggcttatg aaacaggtaa aggttcgatt gtcctacgtt ctcgtacaga gattgaaacg
     5941 actaagactg gtcgtgagcg catcgttgta acagaatttc cttacatggt caataaaacc
     6001 aaggtgcatg agcatattgt tcgcttggtt caggaaaaac gcattgaggg tatcacagca
     6061 gtacgtgatg agtcaaaccg tgaaggtgtt cgatttgtta ttgaagtcaa gcgcgacgcc
     6121 tcagccaatg ttattctcaa taacctcttc aaaatgaccc aaatgcaaac caattttggt
     6181 ttcaatatgc tcgctatcca aaatggtata ccgaaaattt tgtctcttcg tcagattttg
     6241 gatgcttata tcgagcacca aaaagaagtg gttgttcgtc gtacacgttt tgataaggaa
     6301 aaagcggaag cgcgcgctca tatcttagaa ggtctcttga ttgcgctaga ccatatcgac
     6361 gaagtgattc gtatcatccg tgctagtgaa acggatgcgg aagctcaagc tgagttgatg
     6421 agcaagttta agctttctga acgtcaaagt caagctatcc ttgatatgcg tcttcgtcgt
     6481 ttgacaggtt tggaacgcga taagattcaa tctgagtatg atgacctctt ggctctgatt
     6541 gcggatttag cagatattct tgctaagcct gaacgtgttt ctcaaattat caaagacgaa
     6601 ttggatgaag ttaaacgtaa attttctgat aaacgccgta cagagttgat ggttggacag
     6661 attttgagtc tcgaggatga ggacttgatt gaagaatcgg atgtcttgat taccctttct
     6721 aacagaggct acattaagcg tttggatcag gacgagttca ctgctcaaaa acgtgggggt
     6781 cgtggtgtcc aaggaacggg agtgaaagat gatgactttg ttcgtgagtt agtgtcaact
     6841 agcacacatg atcatctgct cttcttcaca aacaagggac gtgtctatcg tcttaaaggt
     6901 tatgaaattc ctgagtatgg tcggactgcc aaagggctac cagtagtcaa tctcttgaaa
     6961 ttggatgaag acgaaagtat tcagacggtt atcaatgttg agtctgatcg cagtgatgat
     7021 gcttatctct tctttacaac ccgtcacggt attgtgaaga gaactagtgt taaggagttt
     7081 gccaatattc gtcaaaatgg tctcaaagcg ctgaatttaa aggatgaaga tgagttaatc
     7141 aatgtcttgt tggcagaagg agatatggat attatcattg gtaccaagtt tggttatgca
     7201 gttcgcttta atcaatcagc cgttcgtggt atgagccgta tcgccactgg tgtgaaaggt
     7261 gttaaccttc gtgaaggaga cacagttgtt ggtgccagct tgattactga tcaagatgaa
     7321 gttcttatta tcacagaaaa aggatatggt aagcgtacag tcgctactga atacccaaca
     7381 aaaggtcgtg gtggtaaggg aatgcagaca gctaaaatta ccgaaaaaaa tggcttgctg
     7441 gccggtctta tgactgttca aggggatgag gatttgatga ttatcactga tacaggtgtc
     7501 atgattcgaa ccaatcttgc caatatttca caaacaggac gtgcaactat gggagttaaa
     7561 gtaatgcgcc tggatcaaga tgctcagata gtgactttca caacggttgc ggtggcagaa
     7621 aaagaagaag ttgggacaga aaacgaaaca gaaggtgaag cataatgtct caaaaaaata
     7681 ataaaaagaa aaacaagcga aaaaatctgc tgacaaatat cctagcagga tttctgatat
     7741 tactgtcact ggctttgatt tttaatactc aaattcgaaa tattttcata gtctggaata
     7801 ccaataagta tcaagttagc caggtatcaa aagaaaaatt agaagaaaat caggatacag
     7861 aaggcaattt tgactttgat tctgtcaaag ctatctcttc ggaagctgtt ctaacttctc
     7921 aatgggatgc tcaaaaatta ccagttattg ggggaattgc aattcctgaa ttggaaatga
     7981 atttgccgat ttttaaagga cttgataatg ttaatctctt ctacggagct ggtacaatga
     8041 aacgcgagca agtaatggga gaaggaaatt atagtctagc tagtcaccat atctttggtg
     8101 ttgataatgc taataaaatg ttattttctc ctttagataa tgctaaaaat ggcatgaaga
     8161 tttatctaac cgataaaaat aaagtttata cttatgaaat acgtgaagtc aaacgtgtga
     8221 caccggatcg tgttgatgaa gttgatgata gagatggggt caatgaaatc acattagtaa
     8281 cctgtgaaga ccttgctgct acagaacgta ttattgtcaa aggtgatttg aaagaaacaa
     8341 aagattattc acaaacatct gatgaaatcc taacagcttt caa
//
LOCUS       DAVLSL010000087         8332 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLSL010000087 DAVLSL010000000
VERSION     DAVLSL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.088"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            18..1736
                     /locus_tag="VXN30_001464"
     CDS             18..1736
                     /locus_tag="VXN30_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1358072.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            1847..2194
                     /locus_tag="VXN30_001465"
     CDS             1847..2194
                     /locus_tag="VXN30_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1358073.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(2473..3309)
                     /locus_tag="VXN30_001466"
     CDS             complement(2473..3309)
                     /locus_tag="VXN30_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1358074.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(3322..3951)
                     /locus_tag="VXN30_001467"
     CDS             complement(3322..3951)
                     /locus_tag="VXN30_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1358075.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(3961..4602)
                     /locus_tag="VXN30_001468"
     CDS             complement(3961..4602)
                     /locus_tag="VXN30_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1358076.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(4748..5977)
                     /locus_tag="VXN30_001469"
     CDS             complement(4748..5977)
                     /locus_tag="VXN30_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1358077.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELYDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(5967..7133)
                     /locus_tag="VXN30_001470"
     CDS             complement(5967..7133)
                     /locus_tag="VXN30_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1358078.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            7277..8290
                     /locus_tag="VXN30_001471"
     CDS             7277..8290
                     /locus_tag="VXN30_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1358079.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         2646 a         1840 c         1478 g         2368 t
ORIGIN      
        1 aataaaggag aatttgaatg tcttaccaag aaaattacca gaaatgggtt gattttgtgg
       61 agcttcctga ctaccttcgt caagatttgg aaaatatgga cgaaaaaact aaggaagatg
      121 ccttctatac aaatcttgaa tttggtactg caggtatgcg tggcttggtt ggtgctggta
      181 caaaccgtat caacatctac gttgttcgcc aagctactga aggattggct cgtttgattg
      241 agtcaaaagg tggaaacgag aaagaacgcg gtgtagcaat tgcctacgat agccgtcact
      301 tctcacctga gtttgccttt gaatctgcgg cagttcttgc taaacacggc atcaaatctt
      361 acgtatttga aagccttcgt ccgactccag aactatcatt tgcagttcgt catctcaact
      421 gtttcgcagg tatcatggtc acagccagcc acaaccctgc tccatttaac ggttacaagg
      481 tttacggtga agacggtgga caaatgcctc cacacgatgc ggacgctttg actacttata
      541 tccgtgcaat cgaaaaccca tttgcagttg aagttgctga tgtggaaact gaaaaagctt
      601 ctggcttgat tgaagttatc ggcgaagctg ttgacataga ataccttaaa gaggttaagg
      661 acataaacat caacccagcc ttgattgaag aatttggtaa agacatgaag attgtctaca
      721 caccacttca tggtactggt gaaatgttgg ctcgtcgtgc tcttgcccaa gcaggatttg
      781 actctgttca agttgttgaa gcgcaagcaa ctgctgaccc agacttttca actgtaacat
      841 ctccaaaccc agaaagccaa gcagcctttg cccttgctga agaacttggt cgtcaagttg
      901 gtgcagatgt tcttgtcgca actgacccag acgctgaccg tgttggtgtt gaagttcttc
      961 aaaaagatgg tagctacctc aacctttcag gtaaccaaat cggtgctatc atggctaaat
     1021 acatcttgga agctcacaaa aacgctggaa ctcttcctga aaatgccgct ctctgcaaat
     1081 ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag ctacggcgca actatgttca
     1141 acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca agaattcgaa gaaaaacaca
     1201 accacactta catgatggga tttgaagaaa gcttcggtta cttgattaaa ccatttgtac
     1261 gtgataaaga tgccatccaa gccgttcttg tagttgctga acttgctgcc tactaccgtt
     1321 ctcgtggttt gacacttgct gacggtatcg aagaaatcta taaagaatat ggctactacg
     1381 cagaaaaaac aatctctgtt actctttcag gtgtcgatgg tgctgaacaa atcaaagcga
     1441 ttatggctaa attccgcaac aatgctccaa cagaatggaa cgcaacagct atcactgtcg
     1501 tagaagactt caaggcacaa actgctactg ttgctgacgg tactgttaca aacttgacaa
     1561 ctcctccaag tgatgtgttg aaatacacac ttgctgacgg ttcatggatt gccgttcgcc
     1621 cttcaggtac agaaccaaaa atcaagttct acattgcagt tgtaggggaa accaacgaag
     1681 aatcacaagc taagattgct aacatcgaag cagaaatcaa tgcatttgta aaataaagac
     1741 ctataaaaga tgagaccaac caacctcatc ttttttcgta tcaaatctaa gcaattttag
     1801 aaactttatg gcatactaga cttatcaatg aaagcgaggt aatctcatgg aacaattttt
     1861 agataacatc aaagaccttg aagtcactac agttgtgcgt gcgcaagaag ctcttgataa
     1921 aaaagaaact gcaaccttct ttatcggtcg caaaacttgc ccttactgcc gtaaatttgc
     1981 aggtacattg tcaggtgtcg tagctgaaac caaagctcac atttacttca tcaatagtga
     2041 agaaccaagc caactcaatg atttgcaagc attccgctca cgctatggaa tcccaactgt
     2101 accaggtttt gttcatatta cagatggaca aatcaacgtc cgttgcgact cttcaatgtc
     2161 agcacaagaa atcaaagatt tcgcaggatt gtaaaaaatc agctaacaag tcttaaaatc
     2221 acaaaaacgc ataatgtaag gtgcaaaaac cttgatatta tgcgttttat tgttcctaaa
     2281 acagttacat cagtatttca ttacagaaca ttttaatttt ggaagtcaaa tcaattttta
     2341 acaatgtttt agaaatcaca gtgggctatt ccatattcaa ttatccatat tcaatcgcta
     2401 ttatcgtttt cctagattac aaaaaaaact acgttttgct aatcaaaacg cagtttcagc
     2461 cactatttta tcttactgtc cttcttttac ttctttggtt gctacatctt ctccaaacca
     2521 tttttggctg atttcttgga acttgccgtc cttgtaaaga ctagaaaaag cttcatttat
     2581 cttcttaacc aagtttgtat cttccttacg ggctccaacc gcaaaagctt ctgtttctag
     2641 tccaactgta aagacattat aatcgtttaa aacaccttct gcttctaaat aatagtttgc
     2701 atagacacgg tcaatcaata gaccatcaat tcgatcgttt ttcaaatcaa tcaaggcttc
     2761 attaaaggtt tggtattgat tcgcttcctt attagcgaca atattcttca aaagttctgg
     2821 atttgcttca aagtccgcat aaccagatga accagcttga gctcctaatg tctttccagt
     2881 catatccttt gcagtcgtga taccagatga tttcttcgta accaatacct gctcattctt
     2941 catatatgag ttactgaaag ccaccttttc acggcgttcg tctgtagcgg aatagccatt
     3001 ccaaatcaga tcaatcgttc cttttgtcaa ttcagcttct ttcaaatccc aatcaatcgg
     3061 ttgccaattt accgtgattc cgtatttttc aaaaacagct gtagctaaat caatatcaaa
     3121 tcctgcataa gaaccatctt tctgagcaaa tcccattgga acaaaagtac tatcaaatcc
     3181 aatagtaata gacttgttag actggtactt tgaccaatta tctccactag tttcgctaga
     3241 atttttccca caagctacta agaacaaagc agtcatcaga ctgactaata caagcatcca
     3301 ttttttcatc catttttcct cctatttagg ttctactttc aataatacat ctgcgatatt
     3361 ttcagcaaac tgcaaatcat gggtaaccac aatctgggtc atcccaagtt ccctattttg
     3421 caagattagc ttctccactt ccaaacgtaa ttctggatcc agggcagaag ttggttcatc
     3481 gtagccaatg atttctgggt caatcatcat agcacgcgcc aaagccactc gctgcttttg
     3541 cccaccagat agtgagaaag gataggcctc tgcgtgtcct cctagtccta actgttccaa
     3601 gagtccactc gccttcttct cagcctcttc ctgcttcatt cccatggtct tcacaggcga
     3661 taaagtcaaa ttttccagaa ctgatagatg aggaaatagt tgaaaatctt ggaagacaaa
     3721 tcccagtagg ttgcgcttct gcaattcatc cagctctaaa ggttgtccat tataaaagat
     3781 ttgccctgaa tcaatggttt caagacctgc aagcatacgt aaaagagttg tcttacctcc
     3841 accagaaggt ccaacgatag ccaggatttg cttttcagga atacttagac tgaaattaga
     3901 caggatttgt ttgtctccaa agactttatt gatatttcgt aattctaaca tggcagcctc
     3961 ctatctataa taactatact tcttctcaac ttttttggaa ataattgtca caatcccaat
     4021 caaaatcaaa taaatggctc ctgccaagaa cataggaact agactagcat cgcggttagc
     4081 agctgttcga ctagccaaga taaggtctga aattccgaga gcatagacca aagaagtatc
     4141 cttgaccaaa ctcataactt cattaaagac actaggaaga acaatcttgg tcacttgggg
     4201 caagataata tagcgcactc tgtcaaaagg gctaaacttt aagaccttgg cagcctcata
     4261 ctgtcctctt ggaatagtgt caattccccc acggaaaatt tctgcaaaat aagctgcata
     4321 gttgagaaca aaggcaataa tagctgcagg aaggcggtct aaacgaatcc caatacttgg
     4381 gagcacataa tagataaaaa tcagttgcaa gagtaagggg gttcctcgca taacccagat
     4441 gtaaatgtta atcagataat ggaggggctt ccaatggact tgcaaggcaa aggcaatcaa
     4501 aacgcccaag ggaatcgaaa atagcaagac cagtgcaaag acctgtacag tcgtgctcgc
     4561 accattcagt aaactcggta atatctcaaa taaataagac atactgcacc tcctaaaagt
     4621 aattgttcct attatagcat gaataaacaa gattaccaag tatttttgta aaaagattct
     4681 accatataca aataaaaacc gtagctatca aaagagataa actacagttc tatatgttta
     4741 aacattctta caatctctca aacagttctt gcatttgcac atcatctgga accagtttta
     4801 agtaagtgtg agcatggact tttgcttctt caaaatgtcc caattcacgc aagagataga
     4861 tatagtgttc cagaaattct ggattgtcct tcaaatctcc tgtcaactct tgataatact
     4921 cataagcagt atccaaatcg tccatttctt gataagaacg agcaatcatc cacttggtca
     4981 aaagattttc tggctcctca ctctgcaatt ctagaatatc ctcataacgc tcctgctcca
     5041 gataaatagt ggctaaacga agcaagattt cttctgtatc ctcagcgtct tcttttgcag
     5101 taaggagata attttctgca ccactagcat catacaattc ataagaaaat tgtgaagcag
     5161 ctagcaagag gcgagtttca aagggatttt tctctaatcc ttgcttagcg atacgcaggg
     5221 cttcttgaac ttgatgttcc ttatgtaaag cctgactgta cccatactca tagccttcaa
     5281 agtcaggaga aatggtatca agctgcttaa agtagagggt ggctttttga tattcttctt
     5341 gatcaaaata aagactggcc aactcaaaag ctgttaagtc atcgtattct aactccaggg
     5401 ctttttctaa aaactcagta gccgtttcaa atttccctaa ctgagcatag gcaaagccaa
     5461 ttcgttgata ggtggaaatg cccgtttgct cataaatcga gcgattatct aactgggcat
     5521 aggcttgaat agccgcttgg taattttcca actcactatc caactctgcc aaacccaata
     5581 tcaagagaga atcctctgag taggtcaagg cctccaataa tttctcacgt gccacatctg
     5641 tcaaaccttc cagctggtaa aggtctgcct tcagagccaa agacgagaca taccagtcac
     5701 tgtcagcttg gatttcctca agataggtaa aggcttcttc tatttgacca tcctcgctag
     5761 caattgcagc tagattaaga tgaacctctg gaaaatcctc tacaattttc aggtaaattt
     5821 ccttggcctg aggatagaaa ccaatccctt caagataagt tgccaattca tacagaagat
     5881 cacttgaatc attttctaaa gctttggcga aataatgctc agccttagtt aaatcttgct
     5941 cctccaaagc ctgtaacatc tgttgactat tgttcactct tgacttcctc accctctaat
     6001 tcatatagac cactgactac cttataccat tcgaaaatgg ctgaaatgac aaccttagca
     6061 gaggcataaa ccggaatacc aagtaaaact ccccagatac caaacataga tcctgaagtt
     6121 aacaaaacaa agagaacatt aatagggtgg atgtttaatt gacttcccaa aatcaatgga
     6181 gagacaaaac ggccttcaat agtttgttct acgataaaga caatcactac tttcaaaagc
     6241 atgactggac cagcaatcaa acccaatact agagcaggaa gcatggctag aaagctacca
     6301 agataaggga ccagatttaa aataccagca gtaaccccca gcgtaaccgc atagcgtaga
     6361 ccaataatct tgaagaagat gataaacatt actgctacaa taatagccac tgtcacttgc
     6421 cctcgaacat agttggacaa ctgttgattc acatctgata aaacttgtcc aacaggttcc
     6481 ttcaattttc ttggaatgaa ttgggtcaaa tagttacgca agcctttccc atcacgcaag
     6541 agataaaaga gcatgaaagg aacgataatc aaggcaacaa tcacttgaga agccccgcta
     6601 ataaaggcac tcacccagtt gactgcctga gatgaaacct tacttgccaa aactgtagcc
     6661 tggctagaaa aattggtcaa aacttgctct aattgaggtc tgaaatcatc tggcaggtgc
     6721 tgggctacca atccattaac aatcctatct atatcttcta agtaaacagg aacgtttctt
     6781 gcaaaggtca aaacctgacg ttgcagattt ggaatggcga ctgccaagcc ccaaatgata
     6841 aagagagcga tgataacaaa gacaatagtg atagctataa cacgattaac cttatgcttc
     6901 tccatccaat caacaatagg attcaacaaa taatataaca aaccagacaa aatgactggc
     6961 aacatcacaa ctgctaaaaa gtctaaaaca ggtgaaaata gaaaactaat cttacttaaa
     7021 ataaaaagat tcagtcccaa taataaggtt actaaaaata ccgtaattgc cttgttatct
     7081 aaaaaccact tgaaaaacca agataggcta aaatgtttct ctttttgctc cataaatact
     7141 tcctttctat tgttctctca ttataccatt tttgaaacaa aataactctt ttttaaagtg
     7201 cttacatcta attggatatc tttaatttac ttgtttttgt gatataatag tcttatcttt
     7261 acacagaggt atagtaatga aagaaactgt ttattttgga acttatacac gtcgtacttc
     7321 tcaagggatt tacaaggcag actttgatac agaaactggt cagctttcaa atctagaact
     7381 ttttgcagct gagccaagtc caacctacct tgcctttgac cagcaccaac atttatacac
     7441 tgttggtagc caagacgata aggggggaat tgcagcctat caaactgacg ggactgtgtt
     7501 aaatcatgtt gttgaagaag gagctcccca ctgttatgtt gctgtcgatg aaaagcgtga
     7561 tttggtttac gcagccaact atcacaaggg acaagtcctt gtttataaac gccaggaaga
     7621 tggtagtctt ctacttagtg atatggatca acacagtggc caaggtccac atgaaaatca
     7681 agcttccccc catgttcact atacagattt aacacctgac cactatctag tgacctgcga
     7741 cttgggaact gaccaagtca tcacctatga cctcgatcaa gaaggaaaat tatctaagct
     7801 ctatacctat cacagcaagc caggagcagg ctcacgccat atcattttcc ataaccacta
     7861 taaaatcgct tatctcattt gtgaactcaa tagtactatc gaagttttaa tctacgatgg
     7921 cgttggcgaa tttgaacgta tgcaggttat ttcaacttta ccagaagctt acgaaggctt
     7981 taatggaacc gctgctattc atctctctaa agacggtaaa tacctctacg cttctaaccg
     8041 tggccatgat tctatcgcag tatataccat ccttgcggac ggtagcttag agttattaga
     8101 aatcgttcca acgcatggtc agactccacg tgattttgat ttgacacccg accaaaaatt
     8161 tctcattgtt gtccatcaag actctgacaa tgcaactgtc tttaaacgta attgtgacaa
     8221 tggtcgtcta gcagaactct ccaacgactt ccatgttccc gaagcagtct gcatccgttt
     8281 tgctccttaa aacaccataa aaaatgaact tggtattttc aagttcattt tt
//
LOCUS       DAVLSL010000088         8286 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLSL010000088 DAVLSL010000000
VERSION     DAVLSL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.089"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            16..462
                     /gene="argR"
                     /locus_tag="VXN30_001472"
     CDS             16..462
                     /gene="argR"
                     /locus_tag="VXN30_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1358080.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            513..3047
                     /gene="mutS"
                     /locus_tag="VXN30_001473"
     CDS             513..3047
                     /gene="mutS"
                     /locus_tag="VXN30_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1358081.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            3194..4888
                     /gene="patA"
                     /locus_tag="VXN30_001474"
     CDS             3194..4888
                     /gene="patA"
                     /locus_tag="VXN30_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1358082.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(5218..5481)
                     /locus_tag="VXN30_001475"
                     /pseudo
     CDS             complement(5218..5481)
                     /locus_tag="VXN30_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5679..7445
                     /gene="patB"
                     /locus_tag="VXN30_001476"
     CDS             5679..7445
                     /gene="patB"
                     /locus_tag="VXN30_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1358083.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            7584..8273
                     /locus_tag="VXN30_001477"
     CDS             7584..8273
                     /locus_tag="VXN30_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1358084.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
BASE COUNT         2320 a         1600 c         1958 g         2408 t
ORIGIN      
        1 acgcaagagg attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact
       61 gaggagaaat taagtacaca aaaagaaatt caagatcggt tggaggcgca caatgtttgt
      121 gtgacgcaga caaccttgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa
      181 aatgatatgg tgtattatgt actagtaaat gagacagaaa agattgattt ggtggaattt
      241 ttgtctcatc atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa
      301 ttgggagaag cctctgtttt ggcaaatatt gtagatgtaa acaaggatga atggatttta
      361 ggaacagttg ctggtgccaa taccttattg gttatttgtc gagatcagca cgttgccaaa
      421 ctcatggaag atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct
      481 caagacttat ttttgaaaag gagagacaga aaatggcgat agaaaagcta tcacccggca
      541 tgcaacagta tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcggatgg
      601 gtgattttta tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt
      661 ccttaacgag tcgcaacaag aatgccgaca atccgatccc tatggcgggt gttccctatc
      721 attctgccca acagtatatc gatgtcttga ttgagcaggg ttataaggtg gctatcgcag
      781 agcagatgga agatcctaaa caagcagttg gggttgttaa acgagaggtt gttcaggtca
      841 ttacgccagg gacagtggtc gatagcagta agccggacag tcagaataat tttttggttt
      901 ccatagaccg cgaaggcaat caatttggcc tagcttatat ggatttggtg acgggtgact
      961 tttatgtgac aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aacctcaagg
     1021 ctcgagaagt ggtgttgggt tatgacttgt ctgaggaaga agaacaaatc ctcagccgcc
     1081 agatgaatct ggtactctct tatgaaaaag aaagctttga agaccttcat ttattggatt
     1141 tgcgattggc aacggtggag caaacggcat ctagtaagct gctccagtat gttcatcgga
     1201 ctcagatgag ggaattgaac cacctcaaac ctgttatccg ctacgaaatt aaggatttct
     1261 tgcagatgga ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgctcaggta
     1321 agaaacaagg cagtcttttc tggcttttgg atgaaaccaa aacggctatg gggatgcgtc
     1381 tcttgcgttc ttggattcat cgccccttga ttgataagga acgaatcgtc caacgtcaag
     1441 aagtagtgca ggtctttctc gaccatttct ttgagcgtag tgacttgaca gacagtctca
     1501 agggtgttta tgacattgag cgcttggcta gtcgtgtttc ttttggtaaa accaatccaa
     1561 aggatctctt gcagttggcg actaccttgt ctagtgtgcc acggattcgt gcgattttag
     1621 aagggatgga gcaacctact ctagcctatc tcatcgcaca actggatgca atccctgagt
     1681 tggagagttt gattagcgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg
     1741 gaattatccg gactggattt gatgagactt tagacaagta tcgttgcgtt ctcagagaag
     1801 ggactagctg gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc
     1861 tcaagattga ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactgg
     1921 gaaatgtgcc agcccacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa
     1981 ccgaagaatt agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc
     2041 tcgaatacga aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac
     2101 aagctctagc ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg
     2161 aaacccagca tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag
     2221 ggcgccatgc tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc
     2281 agatggcaga agataccagt attcaactgg ttacagggcc aaacatgagt gggaagtcta
     2341 cctatatgcg tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg
     2401 ctgaaagcgc ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg
     2461 acttggtttc gggtcagtca acctttatgg tggagatgat ggaggccaat aatgccattt
     2521 cgcatgcgac caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt
     2581 atgacgggat ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta
     2641 agaccctctt tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact
     2701 tggtcaatgt ccacgtggca actttggagc aggatgggca ggtcaccttc cttcacaaga
     2761 ttgaaccggg accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc
     2821 cagcagacct tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag
     2881 agagtcctcc tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata
     2941 gggcagaaga gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac
     3001 ctatgcaggt tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac
     3061 tagttaatct agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat
     3121 aacatcacac aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt
     3181 ttaaggagaa agtatgctga ttcagaaaat aaaaacctac aagtggcagg ccctggcttc
     3241 gctcctgatg acaggcttga tggttgctag ttcacttctg caaccgcgtt atctgcagga
     3301 agtcttaggc gccctccttg ctgggaaata tgaagctatt tatagtatcg gggcttggtt
     3361 gattggtgtg gccgtagtcg gtctagttgc tggtggactc aatgttgtcc tcgcagccta
     3421 tattgcccaa ggagtttcat ccgaccttcg ggaggatgcc ttccgtaaaa ttcaaacctt
     3481 ttcttatgct gatattgaac aatttaatgc gggaaatcta gtcgttcgaa tgacaaatga
     3541 tatcaaccag attcagaacg ttgtcatgat gaccttccag attcttttca gacttcccct
     3601 cttgttcatc ggttcgttta tcctagcggt tcaaacctta ccttctctgt ggtgggtgat
     3661 tgttctcatg gtagtcttga tttttggttt gactgctgtc atgatgggaa tgatggggcc
     3721 tcgttttgcc aagtttcaaa cccttcttga gcgcatcaat gccattgcca aggaaaattt
     3781 acgtggcgtt cgtgtggtca agtcctttgt ccaagaaaaa gagcaatttg ctaagtttac
     3841 agaggtctca gacgagcttc ttggtcaaaa cctttacatt ggttatgcct tttcagtagt
     3901 ggaacccttt atgatgttgg ttggttacgg ggcggtcttc ctctctattt ggctggtcgc
     3961 gggaatggtt cagtcggatc cgtctgttgt tggttccatc gcttcttttg ttaattacct
     4021 aagccagatt atctttacca ttgttatggt tggatttttg ggaaattctg tcagccgtgc
     4081 catgatttcc atgcgtcgta ttcgagaaat tcttgacgca gagccagcta tgaccttcaa
     4141 ggatatccca gatgaagagt tggttggaag ccttagcttt gaaaatgtga cctttaccta
     4201 tccaatggac aaggaaccga tgctgaaaga tgtgagcttt actattgaac ctggtcaaat
     4261 ggttggtgta gttggagcga ctggtgcagg aaagtcaacc ttggctcaat tgattccacg
     4321 tctctttgat ccacaggaag gggctatcaa aatcggaggt aaggatattc gagaagtgag
     4381 tgaaggaacc ctgcgtaaaa cagtttccat cgttctccaa cgtgccattc tttttagtgg
     4441 aacgattgca gataacttga gacaggggaa gggaaatgct actctatttg aaatggagcg
     4501 cgcagccaat attgcccagg ctagtgaatt cattcatcgt atggagaaaa cctttgaaag
     4561 tccagttgaa gaacggggaa ccaatttctc tggtggacaa aaacaaagga tgtcgattgc
     4621 gcgtgggatt gtcagcaatc cacgtattct gatttttgat gattcgacct cagccttgga
     4681 tgccaaatca gagcgcttgg tgcaagaagc tttgaataag gacttgaagg ggacgacaac
     4741 cattattatt gctcaaaaaa ttagctcggt tgtccatgca gacaagatct tggttctaaa
     4801 tcaaggacga ttgattggtc aaggcacgca tgcagacttg gttgccaaca atgccgttta
     4861 ccgtgaaatc tatgaaacac agaaatgaaa gacaaactat aagaaaagtc aatagtttta
     4921 tctaaactat ttcttatttc aatttgatga tttggcgatg attttagagc acggcaaaaa
     4981 gctattgaaa aagtccattt tttcaaaggt aatcctgtgt taatttcaga aattacatca
     5041 ctttttgttc gtcaaatggc agctcttttt taggatataa aacagggttc ggataagttt
     5101 ttttgcaagg tggatgatgg ctacattgta atgttttcct tgttctaact tagtcttaag
     5161 ataggcctta aaagcaggtg aaaagcgagg gcatgctttg gcagcttgta tgagtaccta
     5221 ccgcagatga ggggaacccc gtttgaccat tcttcctgct aaatcaatct gacctgactg
     5281 ataaatagaa gaatccagtc cagcgaaagc ttgtaattga gcaggattat caaaggcatg
     5341 aatatttcga atctcggcta aaatgaccgc ccctaaacga ttctcaatcc cagtaaccgt
     5401 cgtgatgacc gagttgaact cagccatcaa gtcattgaca cattttttcg ccttgtcaat
     5461 gagcctcttg taatgtttga tgttttcatt acaagagata aaacgtttat gcgttatcaa
     5521 actcattacc aattgaaaca aaaagctgtg gttagatcct ttcggaaatt gtcaagcgat
     5581 tggaggaaat gaactaatcc acagcggctt attccaagta taccacttgg gctttggcag
     5641 tagctaactg cgctaaatat aatataagga ggagtaaaat gaagacagtt caattttttt
     5701 ggcattattt taaggtctac aagttctcat ttgtagttgt catcctgatg attgttctgg
     5761 cgacttttgc ccaagccctc tttccagtct tttctggaca agcggtgacg cagctagcca
     5821 atttagttca agcttatcaa aatggcaatc cagaacttgt atggcaaagc ctatcaggaa
     5881 tcatggtcaa tcttggcctg ctggttttgg ttctatttat ctctagtgta atatacatgt
     5941 gtctcatgac gcgcgtgatt gcagaatcga ccaacgagat gcgcaaaggc ctctttggta
     6001 agcttgctca gttgacggtt tctttctttg accgtcgaca agatggcgat atcctgtctc
     6061 attttaccag tgatttggat aatatcctcc aagcctttaa cgaaagcttg attcaggtca
     6121 tgagcaatat tgttttatac attggtctga ttcttgtcat gttttcgaga aatgtgacgc
     6181 tggctctcat caccattgcc agcaccccat tggctttcct tatgctgatt ttcatcgtga
     6241 aaatggcacg taaatacacc aacctccagc agaaagaggt agggaagctc aacgcctata
     6301 tggatgagag catctcaggc caaaaagccg tgattgtgca aggaattcaa gaggatatga
     6361 tggcaggatt tcttgaacaa aatgagcgcg tgcgcaaggc aacctttaaa ggaagaatgt
     6421 tctcaggaat tcttttccct gtcatgaatg ggatgagcct gattaataca gccatcgtca
     6481 tctttgctgg ttcggctgta cttttgaatg ataagtctat tgaaacaagt acagccctag
     6541 gtttgattgt tatgtttgca caattttcac agcagtacta ccagcctatt atccaagttg
     6601 cagcgagttg gggaagcctt cagttggcct ttactggagc tgaacgaatt caggaaatgt
     6661 ttgatgcaga ggaggaaatc cgacctgaaa aggctccaac cttcactaag ttgcaagaaa
     6721 gtgttgaaat cagtcatatc gatttttcat acttgcctga taaacctatt ttgaaagatg
     6781 tcagcatttc tgcccctaaa ggccagatga cagcagttgt tgggcctaca ggttcaggaa
     6841 aaacgactat tatgaacctc atcaatcgct tttatgatgt tgatgctggt ggtatttatt
     6901 ttgatggtaa agacattcgt ggctatgact tagatagtct tagaagcaag gtgggaattg
     6961 tattgcaaga ttcggtcttg tttagcggaa cgattcgaga caatatccga tttggtgtgc
     7021 cagatgctaa tcaggaaatg gttgaggtag cagcaaaagc aacccacatt cacgactata
     7081 tcgaaagttt gcctgataag tacgatactc ttattgatga tgaccagagc atcttttcaa
     7141 cagggcagaa gcaattgatt tcaatcgctc gaaccctgat gacagatcca gaagttctca
     7201 ttctcgatga agcaacttca aacgtagata cggtgacgga aagcaagatt cagcatgcca
     7261 tggaggtggt tgtagcaggt agaactagtt tcgtcattgc ccaccgcttg aaaaccattc
     7321 tcaatgcaga tcagattatt gtccttaaag atggagaagt cattgaacgt ggtaaccacc
     7381 atgaactttt gaagctaggt ggcttttatt cagaactcta tcacaatcaa tttgttttcg
     7441 aataagaaag aagttgtcct atgtgggcag ctttttcttg tccataaaaa atgtttatca
     7501 cagccttaaa aaaaacatat tagacgaaag tcattttgag tgatatgata ggactatcgt
     7561 tagtattcga aaggagaggc atcatggcta gaacggttgt aggagttgct gcaaatctat
     7621 gtcccgtaga cgcagaaggc aaaatcattc attcatctgt atcttgtaga ttcgcagaga
     7681 tcattcgtca agtcggtggt ctccctttag tcattcctgt tggtgatgag tcagttgtac
     7741 gtgattatgt ggaaatgatt gacaaactca ttttgacagg aggccaaaat gttcatcctc
     7801 agttttatgg agagaaaaag accgtcgaaa gcgatgatta caatctggtc cgtgacgaat
     7861 ttgaattggc actcttgaag gaagcgcttc gtcagaataa accaattatg gcaatctgtc
     7921 gcggtgtcca acttgtcaat gttgcctttg gtggaaccct caatcaagaa atcgaaggtc
     7981 actggcaagg actacctttc gggacatctc actctattga gacagtagaa ggaagcgtgg
     8041 tggctaagct atttgggaaa gaaagtcagg tcaactcagt ccatcgtcaa agcattaaag
     8101 atttggcacc taatttccgt gtaactgcta ttgattcaag agaccaaact attgaagcga
     8161 ttgagtctat cgacgagcac cgcattatcg gtttacagtg gcatccagag tttctggtta
     8221 atgaagaaga tggcaattta gaattatttg agtatttatt gaatgaactg taacgattgg
     8281 aacatc
//
LOCUS       DAVLSL010000089         7967 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLSL010000089 DAVLSL010000000
VERSION     DAVLSL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.091"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            189..770
                     /locus_tag="VXN30_001478"
     CDS             189..770
                     /locus_tag="VXN30_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1358085.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            767..2044
                     /locus_tag="VXN30_001479"
     CDS             767..2044
                     /locus_tag="VXN30_001479"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1358086.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYCPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            2062..2724
                     /gene="deoC"
                     /locus_tag="VXN30_001480"
     CDS             2062..2724
                     /gene="deoC"
                     /locus_tag="VXN30_001480"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1358087.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            2711..3100
                     /locus_tag="VXN30_001481"
     CDS             2711..3100
                     /locus_tag="VXN30_001481"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1358088.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            3190..4242
                     /locus_tag="VXN30_001482"
     CDS             3190..4242
                     /locus_tag="VXN30_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1358089.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            4386..5921
                     /locus_tag="VXN30_001483"
     CDS             4386..5921
                     /locus_tag="VXN30_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358090.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            5914..6972
                     /locus_tag="VXN30_001484"
     CDS             5914..6972
                     /locus_tag="VXN30_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358091.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KEFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            6975..7931
                     /locus_tag="VXN30_001485"
     CDS             6975..7931
                     /locus_tag="VXN30_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358092.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2243 a         1438 c         1912 g         2374 t
ORIGIN      
        1 ataaactttt tgtttttatc tggactgtga tatttatagt tctaaatggt ttattattta
       61 taagtgttga aagagattga agaaaatggc ttcgtgactc gctttcttcg gtctttttcg
      121 ttttattatc aatttgtaaa cgatttacaa tttgagtcaa attatggtaa aatagtgtca
      181 tgagtaaaat gtattatgca gaaaatcctg acgctgctca cgacattcat gagttgagag
      241 tagagttgtt gggacaaaaa atgacctttt taacggatgc gggtgttttt agcaagaaaa
      301 tagttgactt tggaagtcaa ctcttgctca agtgtcttga ggtcaaccaa ggagaaactg
      361 tccttgatgt aggctgtggt tacggacctt tgggtttgtc cttagctaag gcttatggag
      421 ttcaggctac tatggttgat atcaacactc gtgccttgga tttagctcgg agaaatgctg
      481 aaaaaaataa tgcaaaagcg acgattttcc agtccaacat ctatgaacaa gttgagggac
      541 attttgatca tgtcatttcc aatcctccta tccgtgcggg caagcaagtc gttcatgaaa
      601 tcattgagaa aagtaaagat ttcttggaaa ctggtgggga tttgacaatc gtcatccaga
      661 aaaaacaagg ggctccaagt gctaagtcca agatggagga tgtgtttggt aattgtgaaa
      721 tccttaaaaa agacaaagga tactatatcc ttagaagtgt gaaagaatga gagcagttga
      781 tttaatacaa aaaaaacgag acggtcaaga actgacttca agtgaaattg aatggctgat
      841 agaaggctat gtgtcaggaa ctgttcctga ttatcagatg tctgcctttg ctatggctgt
      901 ttattttaaa ggaatgacga ctcgtgaaat ttctgattta acgatgaata tggttaagac
      961 tgggcaagag tttgacctgt cagccattga tggtgtgaaa gttgacaagc attctactgg
     1021 tggtgtcggc gataaggtaa ccttgatttt agctcctctt gttgctagct tcggtgtgcc
     1081 tgtagcaaaa atgagtggtc gtggtcttgg ccatacaggc ggaacaattg ataaattgga
     1141 gtccattaag ggctatcaag tagaacgtag tcaagaggat tttattcgtc aggttcagga
     1201 tattggagta tctgtcattg ggcaatcaga ccagctggtc aaagcagata agcttctcta
     1261 tgcccttcgt gatgtgaccg caactgtcga cacgattcct ttgattgcga gttcggtgat
     1321 gagcaagaaa attgcggcag gggcggatgc tattttgcta gacgtgactg tcggtgaggg
     1381 tgccttcatg aagacggttg atgaggcgcg tgagttggct caaactatgg tagatcttgg
     1441 taaggttgtt ggacggaaga cagtagcagt gattacggat atgagccagc ctttgggacg
     1501 agcgattgga aatcgtctgg aaatccttga agcattggag attttgcaag gtcaaggacg
     1561 tcaggatatt acccacttta tttgtgaatt ggctcaaatt atgcttggtt tggcaaatgt
     1621 taacaaaact gttgaagaag ttcgccaaca tcttgagaat ggtcaagcac tggctaagtt
     1681 tgaggaaatg gtgcaagccc aaggtggaga cttggaagac ctctattgtc ctgtaaatgt
     1741 agctcatgtg gtggaaatcc ctgctcagga aacgggtgtc atttcagctc ttccagctat
     1801 ggactttggt ctttatgcca tgaggctggg tgctggtcgt gcagttaagt ctgatgcctt
     1861 ggactatgaa acaggaattg tttttgaaaa gaagattgga gactccgttc aaaagggtga
     1921 aattgttgca aaagtataca caaatgaaaa aattccacct caactagtta cagatttcca
     1981 aaaatgtgtt aaaataagtg atgaagtgaa aaaaatccgt gaaattgtag aaatcatctc
     2041 ctaaatggag ggaaaaccga aatgaaatta aataaatata tagatcatac gcttttaaaa
     2101 caagatgcaa agaaaaaaca aattgatagt ttgttgtctg aggctagaga gtatgacttt
     2161 gccagtgttt gcgttaatcc gacctgggtt gaacatgcta aaaaaggact tgaaggcaca
     2221 gatgttaagg tttgcacagt agtaggtttc cctttgggag caacaacttc agccgtgaaa
     2281 gcatttgaga caaaagaagc tatccaaaat ggtgcagatg agattgatat ggtgatcaat
     2341 gttggagctc tcaaatcagg taatttagcc ttggttgagt cagatattcg cgcagtagtg
     2401 gaagcaagtg gtgataagtt agtgaaagtc attattgaag cttgccttct gacagaccaa
     2461 gaaaaaattg ttgtttgcca attggcccaa aaagctgggg ctgactttgt taaaacatct
     2521 actggttttt caactggtgg tgctacgata gcagatgtta cattaatgcg tgaaacagtt
     2581 ggatctgata tgggagtcaa ggctgctggt ggagctcgtt cttatgcaga tgctcttgcc
     2641 tttgtcgaag caggtgcgac ccgtatcgga acgtcagctg gggtagctat tttaaaagga
     2701 gaattggcag atggcgacta ctgagttgat tgaactggca attgaaacca gcaaacatgc
     2761 ctatgtcccc tattctcact ttcctatcgg agcagtttta gtagccaaag acggaagtgt
     2821 ttacacggga gtgaatatcg aaaatgctag ctatcctttg accaattgcg gtgagagaac
     2881 agctgttttt aaggctatat ctgaaggcca aagagaattt tcagaattga ttgtctatgg
     2941 tcaaactgaa aaaccgatct cgccatgtgg tgcttgtcgc caagtaatgg tcgaattttt
     3001 tgaacaagat ttaaaagtga ccttagtcgc aaaagataaa tcgacggtcg agatgacggt
     3061 cggggaatta cttccatact cttttacaga cttaaactag tctgagtcgc tctctgagtg
     3121 gcacggtcct tgtggccaac caatccatac ttgcaacatc gttgcacatc ttatttagga
     3181 ggttcagtaa tgaacaagaa acaatggcta ggtcttggcc tagttgcagt ggcagcagtt
     3241 ggacttgctg catgtggtaa ccgctcttct cgtaacgcag cttcatcttc tgatgtgaag
     3301 acaaaagcag caatcgtcac tgatactggt ggtgttgatg acaaatcatt caaccaatca
     3361 gcttgggaag gtttgcaggc ttggggtaaa gaacacaatc tttcaaaaga taacggtttc
     3421 acttacttcc aatcaacaag tgaagctgac tacgctaaca acttgcaaca agcggctgga
     3481 agttacaacc taatcttcgg tgttggtttt gcccttcaca atgcagttga agaagcagct
     3541 aaagaacaca ctgacttgaa ctatgtcttg attgatgatg tgattaaaga ccaaaagaat
     3601 gttgcgagcg taactttcgc tgataatgag tcaggttacc ttgcaggtgt ggctgcagca
     3661 aaaacaacta agacaaaaca agttggtttt gtaggtggta tcgaatctga agttatctct
     3721 cgttttgaag caggattcaa ggctggtgtt gcgtcagtag acccatctat caaagtccaa
     3781 gttgactacg ctggttcatt tggtgatgcg gctaaaggta aaacaattgc agccgcacaa
     3841 tacgcagccg gtgcagatat tgtttaccaa gtagctggtg gtacaggtgc aggtgtcttt
     3901 gcagaggcaa aatctctcaa cgaaagccgt cctgaaaatg aaaaagtttg ggttatcggt
     3961 gttgatcgtg accaagaagc agaaggtaaa tacacttcta aagatggcaa agaatcaaac
     4021 tttgttcttg tatctacttt gaaacaagtt ggtacaactg taaaagatat ttctaacaag
     4081 gcagaaaaag gagaattccc tggcggtcaa gtgatcgttt actcattgaa ggataaaggg
     4141 gttgacttgg cagtaacaaa cctttcagaa gaaggtaaaa aagctgtcga agatgcaaaa
     4201 gctaaaatcc ttgatggaag cgtaaaagtt cctgaaaaat aatggatgga agtcatttct
     4261 taggaagcgg ccaagggccg cttttttaag agttgagaca aagtcatttt gtctcagtaa
     4321 agcttgctac tagacaaact agcaagtttt attgaaataa aataagactc tgaaaggaag
     4381 agcacatggc acacgaaaat gtcattgaga tgcgtgatat taccaaggtg tttggtggat
     4441 ttgttgccaa cgacaaaatc aacttgcacc tacgaaaagg tgaaattcat gcacttttag
     4501 gagaaaatgg ggctggtaag tccacgctaa tgaacatgtt agcaggcctt cttgaaccaa
     4561 ctagtggtga aatcgcggtc aacggtcaag ttgtcaatct cgactcccca tctaaagcag
     4621 ctagcttggg aatcgggatg gttcaccagc actttatgtt ggttgaagcc ttcacagtgg
     4681 ctgaaaacat cattttaggt agtgaattga ctaaaaatgg tgtgctagat atcgctggag
     4741 ctagcaaaga aatcaaggct ctttctgaac gttatggctt agctgttgac ccttctgcca
     4801 aggtagcaga tatctcagtt ggagcccaac aacgtgtaga aattttaaaa acactttata
     4861 ggggggctga tatccttatc tttgacgaac caacggctgt tttgactcca tcagaaattg
     4921 atgagttgat ggctattatg aaaaatcttg tcaaagaagg aaaatcaatt atcttgatta
     4981 cccacaaatt ggatgaaatt cgcgcagttt ctgaccgtgt tacagttatc cgtcgtggga
     5041 aatcaattga aaccgttgaa attgcagggg ctaccaatgc tgatttggcg gaaatgatgg
     5101 taggacgttc tgtttccttt aaaacagaga agcaagcctc taaaccaaaa gaagtggttt
     5161 tgtctatcaa agatttggtg gtcaatgaaa accgtggtgt tccagctgtt aaaaatctgt
     5221 ccttggatgt tcgtgctgga gagattgttg gtattgcggg gattgatgga aatggtcagt
     5281 ctgaactgat tcaagccatt acaggtcttc gtaaggttga atctggtagc attgagctaa
     5341 aaggagattc aattgtaggc ttgcacccac gtcagattac agaactaagt gttgggcacg
     5401 ttccagaaga ccgtcaccgt gatggcttga ttttggaaat gatgatatct gaaaatattg
     5461 cccttcaaac ctactataaa gaaccacata gtaaaaatgg aattttgaat tattcaaata
     5521 ttacttctta tgctaaaaag ctgatggaag agtttgatgt tcgcgctgcc agtgaattag
     5581 ttcctgcagc tgcactctca ggaggaaatc aacaaaaagc aattattgct cgtgaaattg
     5641 atcgagatcc tgatctcctt atcgttagcc agccaactcg tgggttggat gtcggtgcca
     5701 ttgagtatat ccacaaacgc ttgattgaag agcgtgataa tggcaaggct gtccttgttg
     5761 tcagctttga attggatgag attttaaacg tctcagaccg tattgccgtt atccacgatg
     5821 gtaagattca aggtattgta tcaccagaaa caaccaataa acaagaactt ggtgtcttga
     5881 tggctggtgg aaacttggga aaggagaaga gtgatgtcta aaaaattaca acaaatttcg
     5941 gttcccttga tttctgtatt tctaggaatt ttactcggag ccattgtcat gtggatcttc
     6001 ggttatgatg ctatttgggg ctacgaagaa ttgttctata cagcctttgg cagtctgcgt
     6061 gggattggag aaatcttccg tgctatgggt cctctggtct tgattggtct tggttttgcg
     6121 gttgccagtc gagctggttt ctttaacgtc ggacttcctg gtcaggcttt ggcaggttgg
     6181 attctcagtg gttggtttgc gctgtcgcat ccagatatgc cccgtccctt gatgattcta
     6241 gcaaccatcg tgattgcctt gattgctggt gggattgtcg gagcgattcc aggtattctt
     6301 agggcctatc tagggacgtc agaggttatt gtaaccatca tgatgaacta cattgtcttg
     6361 tatgtaggga atgcctttat ccatgctttc cctaaagaat tcatgcaaag tacagattcg
     6421 accattcgtg ttggggctaa tgcaacctat cagacacctt ggttggctga gttgactggt
     6481 aactcacgga tgaatattgg tattttcttt gccatcattg ccgttgcagt tatttggttc
     6541 atgctcaaga aaacaactct tggttttgaa atccgtgcag ttggtcttaa tccacatgct
     6601 tcagaatatg ctggtatttc tgccaagcgg actattatcc tatctatgat tatttcaggt
     6661 gccttggcag gtcttggtgg agctgttgaa ggtttgggaa ccttccagaa cgtctatgtt
     6721 caaggttcgt cattagctat cggatttaac ggaatggcgg ttagtttgct tgcggccaac
     6781 tcaccaattg gtatactctt tgcagccttc ctatttggcg ttctccaagt tggggctcct
     6841 ggtatgaatg cggcgcaggt accatctgag cttgtcagca ttgtaacagc gtctattatc
     6901 ttctttgtca gtgttcatta ccttatcgaa cgctttgtca aaccgaaaaa acaagttaaa
     6961 ggaggtaagt aaagatgtct ataataacct tgctccccct cttggtgtct tctatgctga
     7021 tttactcagc acctctcatc tttacaagta tcggtggtgt tttctctgaa cgtggtggtg
     7081 tggtaaacgt tggccttgaa ggaattatgg ttatgggtgc cttttctgga gttgtcttta
     7141 accttgaatt tgcagaacaa tttggagcag caactccatg gctatccttg cttgtagcag
     7201 gattggttgg tagtgttttc tctatcatcc acgcagcagc gacggttcat ttccgtgcag
     7261 accatgttgt cagcggtacg gtattgaact tgatggcgcc tgccttggct gttttcttag
     7321 ttaaagttct ttataacaaa ggacaaaccg acaacctaag tcaaactttt ggacgctttg
     7381 atttcccagt cttggcaaat atcccagtga ttggtgatat cttcttcaag tcaactagtc
     7441 tacttggtta tctggcgatt gccttctcat tccttgcttg gtttattctc tttaagactc
     7501 aatttggtct tcgtctccgc tctgtcggtg aacatcctca agcagcggat accttgggaa
     7561 tcaacgtcta caagatgaga tatttaggga ttattatttc aggttttcta ggtggaattg
     7621 gcggagcgat ttatgcgcaa tcaatctcag ttaacttctc agtgacaact attgttggac
     7681 ctggatttat cgcccttgct gcgatgatct ttgggaaatg gaatccaatc ggtgctatgc
     7741 tttctagtct cttctttgga ctttcacaaa gtttggctgt tatcggttct caattgccgt
     7801 tcctacaagg agtgccagcg gtttatcttc aaattgcacc ttatgttttg acaattcttg
     7861 tcttagcagc cttctttgga aaagcagtcg caccaaaagc agatggtatc aactacatca
     7921 aatcaaaata agcatacaaa aaaacgtcag tccaatgact gaactgc
//
LOCUS       DAVLSL010000090         7879 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLSL010000090 DAVLSL010000000
VERSION     DAVLSL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.092"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..694)
                     /locus_tag="VXN30_001486"
     CDS             complement(<1..694)
                     /locus_tag="VXN30_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1358093.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEK"
     gene            complement(750..1772)
                     /locus_tag="VXN30_001487"
     CDS             complement(750..1772)
                     /locus_tag="VXN30_001487"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1358094.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(1904..4375)
                     /gene="parC"
                     /locus_tag="VXN30_001488"
     CDS             complement(1904..4375)
                     /gene="parC"
                     /locus_tag="VXN30_001488"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1358095.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQIGIVPILSSYIAHRREVILARSRFDKEKSEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(4795..6738)
                     /gene="parE"
                     /locus_tag="VXN30_001489"
     CDS             complement(4795..6738)
                     /gene="parE"
                     /locus_tag="VXN30_001489"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1358096.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            6874..7515
                     /gene="plsY"
                     /locus_tag="VXN30_001490"
     CDS             6874..7515
                     /gene="plsY"
                     /locus_tag="VXN30_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1358097.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(7558..>7879)
                     /locus_tag="VXN30_001491"
     CDS             complement(7558..>7879)
                     /locus_tag="VXN30_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1358098.1"
                     /translation="EKHYENTILHSDQGWQYQHDSYHRFLESKGIQASISRKGNSPDN
                     SMMESFFGILKSEMFYGYEKSFQSLKQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRT
                     KSFG"
BASE COUNT         2138 a         1798 c         1483 g         2460 t
ORIGIN      
        1 atttttctag ttctttttgg tatttgctat tggattccaa tttttctttt tgccattttt
       61 taaaaacctc gttatattct tttgttgtaa caatatcttt ttgcaatttc attcctttaa
      121 agatatatgg ttccccctta ataccaactt gtgagtatgg ttttgagaat ggtactacgt
      181 tacttacaac tggagaacca ccagatgaag ctgttggcat caataatgaa ctatctgtcg
      241 accaagcttg agctttggca tatttttcat atctcttctc taggtcagtg gtctcagaaa
      301 cagcatcttc taacaatttc ttatatttat ccaaaccagg tttagctaca acatccttat
      361 cttttccttt cgtaatacca aggtgtttca tggcagaacc agattttgga tccataatat
      421 tcaagtaaga cgctggatct tgatagcttg gagcccatcc tgtactattc aaatcatagt
      481 ctttttgaga aggagcaaca ttgccgtatt tatcattttc catcaaacca tcaataacat
      541 ttccaataac gtctgtcctc gatgttcgag tcgctatact gtagcccaat gatgctggat
      601 ctactgcata gacataagaa aatgttgtcg gtgcatctgc ttctttatca gtttttccac
      661 aagccactaa aatagctgac gtgctcagga ccactcctgc tgttaagagc cactttttct
      721 atttcataaa aaatctcctt tggtttattt taatctactt ttacaatcca accttctggc
      781 gcttcaatat cgccaaactg aatacccgtc aattcattat ataatttacg cgtcacagga
      841 cctacttctg tttcactata gaatacatgg aaatcatcac catgttgaat acctccaatt
      901 ggagaaataa ccgctgctgt accacaggca cctgcctcta caaaacggtc aagattatca
      961 attggaacat caccctcaat aggagttaat cccaagcgat gttctgccaa ataaagcaag
     1021 gaatacttgg taatagatgg caagatagat ggactcaatg gtgttacaaa ttcattatca
     1081 gctgtaattc caaagaagtt agctgatccg acttcttcaa tctttgtatg agttgatggg
     1141 tccagataga taacatctga gaaatgacgt gacttggcca tttttcctgg taagagactt
     1201 gcagcatagt ttccaccaac cttagccgca cctgtaccat ttggtgctgc acggtcgtac
     1261 tcatcctgaa tcaagaagtt ggttgggacc aaaccacctt taaagtaatt tccaactggc
     1321 atagcaaaga tggtgaaaat gtactcttct gccggtttta ccccgataat atctccgaca
     1381 ccaatcaaaa gagggcgaag atataaggtt ccacctgttc cgtatggtgg tacgtattct
     1441 tcattcgcac ggacaactgc tttacaagct tctacaaaca tgtctgtcgg aacttgtggc
     1501 atcaagagac ggtcacatgt acgttgcaga cgtttagcat tttcatcagg acggaacagt
     1561 tgaacactgc catccttagt acgataagct ttcaaacctt caaatgcttg ttgtccatag
     1621 tgaagacttg gagaagactc tgaaatatgc aaagttgcat cctctgtaag ctctccttga
     1681 tcccattgtc catttttgaa atgagcaaga tagcgataag gtaatttcat ataggaaaaa
     1741 ccgaggtttt cccaatcaat cgttactgtc atattccact ccttattcta aaaacctatt
     1801 tcttatattc tacactattt ttctaaaata gcaagtatat tttgtaattt tcagaaaatt
     1861 tctccaataa aaaccagctc ttagaactga ttcttcattt cacttattta tcttcagtaa
     1921 ctacttcctg aagataagcg tcaaaaactt cttcatctga aatcgtgtca gaaatgaagc
     1981 ttccattgct agtgcgttct gacaagttca agtcttgcaa tcggctttca tagattgttc
     2041 ctttattgga ttggacaagc agagtttggt cgttcacatc cacttccgta ctgaagaaat
     2101 cgccaacaaa tccttgctct gcaactgctc ctgccaagaa gacacgatgc ggtttgtttt
     2161 tcaactcacg caagacttgt aatcctcgtt tggcacggct ggttgctaga atttcctcaa
     2221 tggaaacacg tttcaagctt ccacgctggg tcaagaggta gaaggacgaa gtattacaga
     2281 taaagccaga ttggaggaca tcatcttctt tcaaattcat agccttgaca cctgctgcct
     2341 tagcaccgac aaccggaacc tcttcgatat tgaaacgcag ggcataacca ttttgactaa
     2401 ccaagacaac atcatctagt ttaatcggag ccactgctac aatctgatct gtatcgtctt
     2461 tgagcttagc atacttgaca gacttagatc tataggtccg ccatggagtg aattcttttc
     2521 gctctacccg tttgatttga ccaaggcgag tcactgcaaa gtaggttgtc gcatcgtcaa
     2581 actgatccag tacttccaca taaaggattt cttcattcgt ttcaaagttt gtgatggttt
     2641 ggctcagatg ctctccgatg tccttccaac gaatatctgc caactcatgg attggtctgt
     2701 agatgacatt tccaagactt gtgaacatca agaggtgctg ggttgtcttg gcagattgaa
     2761 caaaaatcaa acggtcatca tcacgcttgc caatttcttc caaggtggaa gccgcaaagg
     2821 aacgtggact ggtacgcttg atgtaacctg ccttggtcac gctgacgtag gtatcttcct
     2881 cagcgataag actagctgta tcaatctcaa ttgctttcgc agtgtcttct aaagaactca
     2941 aacgaggagt tgcaaatttc ttcttgacct cacgaagttc tttcttcatg agattgtaca
     3001 tagtcctttc atcaccgata atagccgcca gcatagcaat cttctcacga agctctgctt
     3061 cttcttcctg caagacaacc acatcggtat tggtcaaacg gtacagttgc aaagttacga
     3121 tagcctcagc ctgttcttcc gtaaaatcat agctaacttt gaggttttcc ttggcgtccg
     3181 ccttattctc agaagcacgg ataagagcaa tgacttcatc caaaatcgaa atcacacgaa
     3241 tcaaaccttc gacgatatgg agacgtttct cagacttttc tttgtcaaag cgtgaacgcg
     3301 ccaaaatcac ttctcgacgg tgagcgatat agctagacag gattggaaca atcccaatct
     3361 gacgaggtgt gaaattgtca atcgccacca tattaaagtt gtagttgatt tgtaggtcgg
     3421 tgtacttaaa taagtagttg agaacaagct cagtattagc gtctttctta agttcgatag
     3481 cgatacgaag accatcacgg tcagactcat cacgaacctc agcaatccca gctaccttgt
     3541 tattaacacg aacatcatcg attttcttga ctagattggc cttattgatt tcataaggaa
     3601 tctcagtaat aacgatttgt tccttaccac cttttagctt ttcaatttca gtcttggaac
     3661 gaacaaccac gcgccctttc ccagtctcat aagctttctt gatttcatca cgaccctgaa
     3721 taatagcccc tgtagggaag tctggtccag gcaagaattc catgagttta tcaatctttg
     3781 cagttgggtg gtcaatcatg taaactgcag catctatgac ctcagctaaa ttatggggag
     3841 gaatgtctgt ggcataacca gccgaaatcc cagtcgaacc attgaccaag aggtttggaa
     3901 aggctgctgg caagaccgtt ggttctttct ccgtatcgtc aaagttccat gcaaaaggaa
     3961 ctgtcttttt ctcgatatcc tgaagaaggt agcctgcaat ttcagacaaa cgtgcctcag
     4021 tataacgcat agccgcagga ggatctccat ccatagaacc gttattaccg tgcatttcaa
     4081 ctagaatctc acgatttttc cagttctgtg acatacgaac catggcatca tagatagaag
     4141 aatccccgtg tgggtggaaa ttccccatga tgttcccgac tgacttggcc gacttacggt
     4201 agctcttgtc aaaagtattg ctatccttat tcatagaata aagaatacga cgttgaaccg
     4261 gcttcaaccc atcacgaata tctggcaaag cccggtcttg aataatgtac ttggagtagc
     4321 gaccaaagcg ctctcccatg atgtcctcca gggacatgtt ttgaatgtta gacataagat
     4381 acaaagccca taaaatacca agtgaaaata gaaaattctt gaagtaagca aactcacaag
     4441 agaatttatc tttttcacac agtatctagg gcgtgttcaa ctcctttcaa agaatgtaga
     4501 gtaggttttt atgcagtaaa agatatttta cgggaattcc tcccgtgttc agttacgata
     4561 agtaaccaaa ctatcctgtt tgtatttttc aatatgaaaa tctggttttc caaaattagt
     4621 cttagtttgt gtcttaaccg ctcccttaag cgcctcttta agataagcac tcatagcaga
     4681 ttcttcatta ataatcctgc aattttttca aaccaagatt ttcaaactgc tttttcacat
     4741 agtcattcac atccgactct aatttccagt ttactaacat attattttct ttcattaaaa
     4801 cactgtcgct tcttctagcg taaacttgac attatcttca atccatttac ggcgtggttc
     4861 taccttatct cccatgagaa cattgacgcg gcgttcggcg cgcgctaaat cttcaattgt
     4921 gacacggatg agggtacgtg tttctgggtt catggttgtt tcccagagct ggtccgcatt
     4981 catctcacca agtcctttgt atcgttggag ggtagcgcct ttaccgaact gtttacggag
     5041 ttcttctagt tctccatccg tccaagcgta ggccacttct tctttcttgc ctttaccttt
     5101 ggacatcttg taaagaggtg ggagggcaat atagacatga cctgcctcga ctagcggacg
     5161 catgtaacgg tagaaaaatg tcaagagcaa ggtctggata tgggcaccgt cggtatccgc
     5221 atcggtcatg ataatgatct tatcatagtt ggcatcttca atagagaagt ctgctccaac
     5281 acccgcacca atggtataaa tcatggtatt gatctcttca tttttgagga tatccgccat
     5341 cttggccttg gctgtattga caaccttacc acgaagaggt agaatagcct ggaacttgcg
     5401 gtcacgacct tgtttggcag aaccaccggc agagtccccc tcaactagat agagttcatt
     5461 cttagcagga ttcttagatt gggctggggt caatttccca gacaacaagc ccttatcttt
     5521 cttgtttttc ttcccatttc ggctctcatc acgcgcctta cgtgctgctt cacgagcatc
     5581 acgggccttg atagccttgc ggatgaggtt agaagctaat tccccatttt ccataaggaa
     5641 aaaggtcaac ttatcagcca ctattccatc cacaactggg cgagctaggg ggcttcctag
     5701 tttatccttg gtctgtcctt caaactgcaa gtgttcttca ggaactaaga tagaaagaac
     5761 ggccgctagt ccctcacgat agtctgaacc ttcaaggttt ttatcttttt ccttgagaag
     5821 acctgtttta cgtgcatagt cattcatgac cttggtaatg gcagacttga gtcctgtctc
     5881 gtgcgttcca ccgtccttgg tgcgaacgtt attgacaaag gatagaatgt tatctgagaa
     5941 tccgtcattg tactggaggg ctacttccac ttgaaaacca ttgtcttccc cttcaaagta
     6001 aagaactggc gtcaagattt ccttatcttc gttgagataa gaaacaaaat cttgtactcc
     6061 attctcatag tggaactcaa tcgcttcatc tgttcgcttg tccgttaaag acaaggtcac
     6121 atttttcaag agaaaggctg attcattaag gcgctctgaa atggtattgt acttgaaatc
     6181 tgtcgtagaa aagatagtcg cgtcaggcat aaaagtaact ttggtgcctg ttttagactt
     6241 gggtgctgta ccgattttct tcaaagtcgt gacaggtttt ccaccatttt caaaacgttg
     6301 cttgtaaact gcgccatcac gggtaatttc aacttctaac cagctagaaa gggcgttaac
     6361 aacggaagaa cccactccgt gaagtccacc tgatgtctta tagccacctt gaccgaattt
     6421 ccctccggca tgaagaatgg taaagataac ctcaacagtt ggaattccca tagcgtgcat
     6481 acctgtcggc atcccacgtc catggtcttg aaccgttaga ctaccgtctt tattgatagt
     6541 tacatcaata cgatcaccaa acccagacaa ggcttcatcg actgcattat caacgatttc
     6601 ccaaactagg tgatgaagac cagcgccatc ggtcgatcca atatacatcc ctggacgttt
     6661 tcggaccgca tccaaccctt ctagcacctg aatagcatca tcattataat tgttaatatt
     6721 gatttccttt tttgacacaa ggaacctcct attcgttcat ctttactatt ctacaggttt
     6781 tccaaggatt ttgcaaaatt tttctttctc cgatgtgaca attttagcag agattctctg
     6841 cttttctttc ccaattcatg atataatagg agtatgatta caatagtttt attaatccta
     6901 gcctatctgc tgggttcgat tccatctggt ctctggattg gacaagtatt ctttcaaatc
     6961 aatctacgcg agcatggttc tggtaacact ggaacgacca ataccttccg cattttaggt
     7021 aagaaagctg gtatggcaac ctttgtgatt gactttttca aaggaaccct agcaacgctg
     7081 cttccgatta tttttcatct acaaggcgtt tctcctctca tctttggact tttggctgtt
     7141 atcggccata ccttccctat ctttgcagga tttaaaggtg gtaaggctgt cgcaaccagt
     7201 gctggagtaa ttttcggatt tgcgcctatc ttctgtctct accttgcgat tatcttcttt
     7261 ggagctctct atcttggcag tatgatttca ctgtctagtg tcacagcatc gatcgcggct
     7321 gttatcgggg ttctgctctt tccacttttt ggttttatcc tgagtaacta tgactctctc
     7381 ttcatcgcta ttatcttagc acttgctagt ttgattatca ttcgtcataa ggacaatata
     7441 gctcgtatca aaaataaaac tgaaaatttg gtcccttggg gattgaacct aacccatcaa
     7501 gatcctaaaa aataaaacgc cagttctgta ctgcccccaa acagttagac aattaattta
     7561 tccgaaggat ttagttctgt attgcacagg gctaagtcct tttagtttta ccttaattct
     7621 cttattgttg taataatcaa tatagtctat aatggcttgt tccaattgct taagcgactg
     7681 aaacgacttc tcataaccgt aaaacatttc cgatttcaga atcccaaaga aggactccat
     7741 catactattg tctgggctgt ttcccttgcg tgatatggat gcttgaattc ccttactctc
     7801 taggaaccga tgataagaat cgtgttggta ttgccagcct tggtcactat ggagaatcgt
     7861 attctcgtag tgcttctct
//
LOCUS       DAVLSL010000091         7440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLSL010000091 DAVLSL010000000
VERSION     DAVLSL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.093"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(179..1018)
                     /locus_tag="VXN30_001492"
     CDS             complement(179..1018)
                     /locus_tag="VXN30_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1358099.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMSFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(1032..1853)
                     /locus_tag="VXN30_001493"
     CDS             complement(1032..1853)
                     /locus_tag="VXN30_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV1358100.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(1990..2847)
                     /locus_tag="VXN30_001494"
     CDS             complement(1990..2847)
                     /locus_tag="VXN30_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1358101.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(3001..3735)
                     /locus_tag="VXN30_001495"
     CDS             complement(3001..3735)
                     /locus_tag="VXN30_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1358102.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(3735..4418)
                     /locus_tag="VXN30_001496"
     CDS             complement(3735..4418)
                     /locus_tag="VXN30_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1358103.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4608..4838
                     /locus_tag="VXN30_001497"
     CDS             4608..4838
                     /locus_tag="VXN30_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1358104.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            5067..7325
                     /locus_tag="VXN30_001498"
     CDS             5067..7325
                     /locus_tag="VXN30_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1358105.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
BASE COUNT         2315 a         1645 c         1349 g         2131 t
ORIGIN      
        1 agaactctga caaatcactt taagtacgga tgtaaaccaa atcaactcta acaaaaatag
       61 atttacaaga gtaaaaaaac gaacaaattt agtttcttag cgagaagaat actaaacgtt
      121 cgttttttgt gaattaagaa taatgataca aattatcaat attttttgtc taactatttt
      181 agacatatct tttcattact ttaggaagac aattactaat ctccgttggc aaaaccacat
      241 actgttcttg agctagttct tgggctatgg ctgaatgaag atgggtcgct actgccacac
      301 gttcgtaaag actagcttgc ttaaactggc ctgcaaatcc tgcaatcatc ccagcaagcg
      361 tatctcccat tcccccagtc gcctgatagg gaccgccaac ctgtaactgg taataatctg
      421 attggccagc ttgccaaata cgagtagctg aacctttctc caccaaaatt gttccttgag
      481 ggaaggaagt tagggcacta gccgttgcat cttccttttg cttttcaatc gtaattccag
      541 ataatttttc ccattctttt tggtgaggag ttaggataag ctggcttgag gaaaatgaca
      601 aacttgtcct agcaagaatg gtcaaggcac ctccgtctac aatcaaaatc tgattttgtc
      661 ttaagccagc aaagacctgt ttaactagat cttctccaaa agcatcgtct cgtaaacccg
      721 gccccagcaa gacaacttct gccttctcca attgctcttt taacaactgc tggtcttgaa
      781 gagaaaagga catagcctca ggtaaatggc tgtgcagggc tggaatattt tccctatctg
      841 ttccaacggt caccaatcct gcaccgcttt ttacagctgc caaagcagcc atgatgatgg
      901 caccaccata aggataagtt ccaccaagaa acagcagacg gccatagtct cctttatgac
      961 tagtacgaga acgttcaata ataacttttt ctagtaaggc ttgatcaatc actttcatcg
     1021 ttcttcctca actatccttt atctgattgt tttaaaacaa cttgatagac tttcttagtc
     1081 cactcttcta agtcttctcc agcatagtca agataatgca ccctatcttg tattttagtt
     1141 ggaataatat tttcaaattg gctcttatta tgagttggaa taatacaaat atacaactca
     1201 ctaaataaat tcaacatgtc cttaattttt gcaacatggt agccatcaaa aatataacca
     1261 gtaactttaa tcaaatctgt atagataaaa tgaaaactca ctccaggaat aaacttgtct
     1321 tgtaattctt tctctgaagg agctcgacct aataaacgct ccatcgctaa tcgataacca
     1381 gaacttgtat ttgaccaccc taacatgaca taatcaaaat aatctatcgg atccgatgca
     1441 gcatttctac tatccaaaac caactcttca gcatctcgcc taaaggcttt cacagcagat
     1501 aagagctgac ctgttttaaa gatagaaatc gctgcatcta tagtcgtagt atgactacag
     1561 tagtctgaag tcactaacct cttatgtaga tcataagtcg aggctactct tttatctgta
     1621 ggggtaaatg gtggcagaaa gggatgctga cagatgtaat ctaaaatttc tttttttaaa
     1681 caagaatcag cacactcaat acttgtttga cgtttatgaa gtttttcgta agctataaat
     1741 ttagcaattt tttctaattc ccactcttga atatttggat aatcaattag tgctaggtag
     1801 tatattccat cccattgtaa actatattcc ctattataaa tttttaaaat catctaatca
     1861 cctcactcca ttatacaaca aaaaggaggc gcagacctcc ttttgtaatc ttatatctaa
     1921 aatttaatat tcatttctgc cattttaaat atagctatag aaaatacact ctcttcagcg
     1981 atttttctct tattttctat ccaatgtccg aagtgctgct tgataagttt gctccatcag
     2041 catagtaatg gtcatagggc cgacacctcc agggactggc gtaatatagc tagcaagtgg
     2101 ggcaactgcc tcataatcca catccccaca gagcttacca ttttcatcac gattcatccc
     2161 aacgtcaatg actaccgcac ctggtttgac aaagtcagca gtcacaaact tggcacgacc
     2221 gattgcgacc accagaatat ctgctttagc agccacctta gcaagattat gggtgcgtga
     2281 gtgggtcaaa gtcactgttg cattcttggc caaaagaagc tgagccatgg gttttccaac
     2341 gatattagaa cgaccgatga caaccgcatt tttaccttct aattcaatcc catattcatg
     2401 aaacatttcc ataattcctg caggtgttga tggaatcatg actggatgtc cagaccaaag
     2461 gcgtcccata tttagaggat ggaaaccatc cacatccttt tctgggtcaa tagccaatag
     2521 aaccgcctct tcatcaatgt gttttggtaa tggcaactga accaaaatcc catgccaagc
     2581 tggatcctga ttgtatttag caatcaggtc taacaattcc tcttgagtaa tggtctctgg
     2641 aactcgcact acttcgctac gggaaccagc cgcaagagct gacctctcct tgttgcgaac
     2701 gtagacttgg ctggctggat tatccccaac caaaatcact accaaaccag gcactagagc
     2761 tgtttcttcc tttaattttg cagtcttttc agccaactgc ccctgcaatt tggccgctaa
     2821 agctttccca tcaataatct gtgtcatatc acttctcttt tctttcaaat atcttccatt
     2881 ataccaaaaa ctgctagtgc cctctaacac ttctttccta ggttatccta tagtttcaaa
     2941 ctataagaaa aagctctggt tgaacttttc actaatcttg tcttgaaatt ttaaaataga
     3001 ttataaaacc ttactcaaga aatctttagt ccgttgttct tgggtttgtt caaaaatctg
     3061 ctcaggtgtc ccatcttcaa caaccacacc gtctgccata aagataacac gatctgccac
     3121 ctcacgggca aatcccatct catgtgttac gataaccata gtcatccctg acttggcaag
     3181 gtcttgcata acagccaata cctcacctac catttcagga tccaaggctg aagttggttc
     3241 gtcaaagagc aaaacatctg gttccatagc caacccacgc gcgatggcaa tccgttgttg
     3301 ctgcccaccc gacagactct gtggataagc gtctgcctta tctggcaaac caactttttc
     3361 caaaagctct tgggctctct tctcagcaac ttccctgctt tcacctttag tcttgatagg
     3421 tgacaaggta atattttcca tcacagtcat attaggaaag aggttaaatt gttggaaaac
     3481 catgcccatc ttctcacgca tggcaaacag gtcattcttc ttgtccgtaa tatcgactcc
     3541 ctcaaagata agcttcccct tggttgcttc ctccaacaaa ttcatagagc gaagcaaggt
     3601 agatttccca ctccctgaag gaccgatgat aacgacaact tctcctcttt taatctcgag
     3661 gttgatgccc ttcaatactt cattctttcc aaaagattta tgtaaatttt caatttttat
     3721 caaggtttct gtcattattt cttatctcct tgtcccatat gtttttcaaa agctttcaag
     3781 gctactgtca gaatagaggt cataatcaag tagtaaaatg ctgcaaataa aagtggtgtt
     3841 aaaggtagat aggttgttgt agaaactgtt gtagccccat tccacaactc catgacccca
     3901 atagctgata agagggagct gtccttgata atggtgataa attcgttccc caatgctggc
     3961 aagatatttt tgactgcttg tggcaaaatc acataacgca tcgcattttt aagacgaatc
     4021 cctagcgaat aagccgcctc tagctgacct tttggaaccg cattgattcc ggcacgaaca
     4081 gtctccgaaa cataagcacc actattcata gagataatca aaatccctgg aatcagacgc
     4141 gaaaaatcaa cacctaaaat tccaatctga atagtcggag cattgatatg cataagagca
     4201 aaggcaatca taatttgaac catcatcggt gtcccacgga aaatccaaac gtacaagttg
     4261 gccaaccaaa caagcggttt aaactttgaa cgttgcccaa aagccaagac aacacccaaa
     4321 atagttccca aaaagataac acagatagaa ataagaatcg tcacaacagc cccatagtta
     4381 aaataaggta aatacttagg taaaaaagaa aaattcatag tcacactgct ttctaaaata
     4441 gaatactgta tgattataac atgttttgaa ttaaaattta aattttttat ccaatttatt
     4501 taaaaatctt taaactagca gaattagcga gaaatcttag ttttttctag tcaagttgcc
     4561 ctcctttatg gtaaaatagt aacgataaga aatgaaggag aatcaaaatg gaatcacatt
     4621 tggttagaat catcaaccgc cttgaagcaa tggcaaacga tggcggaaat ttaaaacgta
     4681 attttgaacg cgaaggagtt gttgttgcag aagttgcgta cagccacgac gaagaaaacg
     4741 gcccaatctt tacccttcgt gacgtagaag atcgcgaaac gtatacgttt gatagcattg
     4801 acttaatcgc aatggaaatt tacgaacttc tctactaatc taaaacaagt tgggattgtc
     4861 cctgcatgtc taagtaaaat cctttttgtc tcacaaatag gattttttta tagtccaaat
     4921 tcacaaaaat tttcattgta acaacttctc aaagcttcaa tctttttact tgctttacac
     4981 cccaatcctg ttataatgag agtatagaat ttgactattt ttgaccttta aacaagtcag
     5041 gctaaagaaa gaaatgaggt agatgtatgc tttgtcaaaa ctgtaaaatt aacgactcaa
     5101 caattcatct ttacaccaat ctcaatggaa aacaaaaaca aattgacctc tgtcaaaact
     5161 gctataagat tatcaaaaca gatcctaaca atagcctctt caaaggtatg acggatctga
     5221 acaatcgtga cttcgatccc tttggtgatt tcttcaatga tctaaacaat ttcagacctt
     5281 ctagcaatac tcctcctatt cccccaaccc aatcaggtgg aggttacggt ggaaacggcg
     5341 gttatggttc ccaaaatcgt ggatctgctc aaactccgcc acctagccaa gaaaaaggcc
     5401 tgctggaaga atttggtatt aatgtaactg aaattgcccg tcgtggagac attgaccccg
     5461 ttattgggcg cgacgatgag attatccgtg tcatcgagat tctcaatcgt agaaccaaga
     5521 ataatcctgt ccttatcggt gaacctggtg tcggaaaaac ggccgttgtc gaaggtctag
     5581 ctcagaaaat tgtcgatggc gatgtgccac ataaactcca aggtaaacaa gtcatccgtc
     5641 tggatgtggt tagcttagtt caaggaacgg ggattcgagg acaatttgaa gaacgcatgc
     5701 aaaaactcat ggaagaaatt cgcaaacgtg aagacatcat cctctttatc gatgaaatcc
     5761 atgaaattgt tggtgctggt tctgcgagtg atggtaatat ggacgcagga aatatcctca
     5821 agccagccct tgctcgtgga gaactgcaac tagtcggtgc tactaccctc aatgaatacc
     5881 gtatcattga aaaggatgct gccctcgagc gtcgtatgca gcctgttaaa gtcgatgaac
     5941 caacggtgga tgaaacaatc actattctca aagggattca aaagaaatac gaagattacc
     6001 accacgttca atataccgat gctgcgattg aagcagctgc aactctttcc aatcgctaca
     6061 tccaagatcg cttcttgcct gacaaggcca ttgacctcct agatgaagct ggttctaaga
     6121 tgaacttgac cttgaatttt gtggatccta aagtaattga tcagcgcttg attgaggctg
     6181 aaaatctcaa gtctcaagct acacgagaag aagattttga gaaggcggcc tacttccgcg
     6241 accagattgc caagtataag gaaatgcaaa agaaaaagat cacagaccag gatactccta
     6301 tcatcagcga gaaaactatt gagcacatta tcgagcagaa aaccaatatc cctgttggtg
     6361 atttgaaaga gaaagaacaa tctcaactca tccatctagc cgaagatctc aagtctcatg
     6421 ttattggcca agatgatgca gtcgataaga ttgccaaggc tattcgccgt aatcgtgtcg
     6481 gacttggtac ccctaaccgc ccaatcggaa gcttcctctt cgttggacca actggtgtcg
     6541 gtaagacaga actttccaaa caactggcta tcgaactttt tggttctgct gatagtatga
     6601 ttcgctttga tatgagtgaa tacatggaaa aacatagtgt ggctaagttg gtcggcgctc
     6661 ctccaggtta tgttggctat gatgaggctg gtcaattaac tgaaaaagtt cgccacaatc
     6721 cgtattctct catccttctc gatgaagtgg aaaaagctca cccagatgtt atgcacatgt
     6781 ttcttcaagt cttggacgat ggtcgtttga cagacgggca aggacgcacc gttagcttca
     6841 aggatgccat cattatcatg acctcaaatg caggtacagg aaagaccgaa gctagcgttg
     6901 gatttggtgc tgctagagaa ggacgtacca attctgtcct cggtgaactc ggtaacttct
     6961 ttagcccaga gtttatgaac cgttttgatg gcattatcga atttaaggct ctcagcaagg
     7021 ataacctcct tcagattgtc gagctcatgc tagcagatgt taacaagcgc ctctctagca
     7081 acaacattcg tttggatgta actgacaagg tcaaggaaaa gttggttgac ctaggttatg
     7141 atccaaaaat gggagcacgc ccacttcgtc ggactattca agactatatt gaggacacaa
     7201 tcactgacta ctaccttgaa aatccaagcg aaaaagatct caaagcagtt atgactagca
     7261 agggaaacat tcagattaaa tctgccaaaa aagctgaagt taaaagttct gaaaaagaaa
     7321 aataaatcct atagaaaagg agtagaaaat gaaatttttc tgcttctttt tttactaaaa
     7381 taactgtaat ttcttgacag cttgcccttt gtccattatg atatatagta gactgaatct
//
LOCUS       DAVLSL010000092         7379 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLSL010000092 DAVLSL010000000
VERSION     DAVLSL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.094"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..297
                     /locus_tag="VXN30_001499"
     CDS             <1..297
                     /locus_tag="VXN30_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1358106.1"
                     /translation="NPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYCRIAEDAK
                     DYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDDGQD"
     gene            281..883
                     /locus_tag="VXN30_001500"
     CDS             281..883
                     /locus_tag="VXN30_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1358107.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            885..1316
                     /locus_tag="VXN30_001501"
     CDS             885..1316
                     /locus_tag="VXN30_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358108.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            1378..2634
                     /locus_tag="VXN30_001502"
     CDS             1378..2634
                     /locus_tag="VXN30_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1358109.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            2642..3616
                     /locus_tag="VXN30_001503"
     CDS             2642..3616
                     /locus_tag="VXN30_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1358110.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            3616..4230
                     /gene="pmp23"
                     /locus_tag="VXN30_001504"
     CDS             3616..4230
                     /gene="pmp23"
                     /locus_tag="VXN30_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1358111.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            4304..5278
                     /locus_tag="VXN30_001505"
     CDS             4304..5278
                     /locus_tag="VXN30_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1358112.1"
                     /translation="MRKKLFLTGAAVLWAVTAMNSAHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVVNDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            5352..6983
                     /gene="rlmD"
                     /locus_tag="VXN30_001506"
     CDS             5352..6983
                     /gene="rlmD"
                     /locus_tag="VXN30_001506"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1358113.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLFRRFDLKPYDEKEHSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            7137..7286
                     /locus_tag="VXN30_001507"
                     /pseudo
     CDS             7137..7286
                     /locus_tag="VXN30_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT         2354 a         1408 c         1543 g         2074 t
ORIGIN      
        1 aatccaccct atatctctcg tgaagatgag tcagaggtag gtttgaatgt tttgcattcg
       61 gagcctcatc tagctctctt tgcagacgag gatggcctag ctatttactg tagaattgcg
      121 gaagatgcaa aagactatct caaggatggt ggtaagattt accttgaaat tggatacaag
      181 caaggtcaaa gtgttcccga actttttaga aaacatcttc ctgaaaaaaa agttcgaaca
      241 ctcaaagacc aatttggtca aaataggatg gttgtggttg atgatggaca ggattagaca
      301 agagttggaa aatggtggag ctgtcgttct acctacagag actgtttatg gtctttttgc
      361 caaggcctta gatgaaaaag cagttgacca tgtttaccaa ctcaaacgtc gtcctagaga
      421 taaggcactc aatctcaata tcgcctcttt cgaggatatc ttgcactttt ctaagaatca
      481 gccaacttat ctacaaaaac ttgtagagac ctttttgcca ggtcccttga ccattattct
      541 cgaagccaat gaccgagttc cctattgggt aaattctgac cttgcaacta ttggatttcg
      601 gatgcccagt caccctatca cactggattt aattcgagag acaggtccct tgattgggcc
      661 gtctgccaat atctcaggtc aggcaagtgg tgtaaccttt gaacaaattc tgaaggattt
      721 tgaccaagag gttctgggtc tggaagacga tgcttttcta actggacagg attcaactat
      781 tgtggatttg tctggagaca aggtgaaaat cttacgccaa ggcgcaatta aacgagaaga
      841 tattcttgct cggttgccag agatttcttt tgaggaggct tgaaatgcta agagatttgc
      901 aagaaacaga tgtgaaagcg atatgtgaca tcaaccaaga ggctttgggt tatactttta
      961 gtccagagga aacggctagc caactagcta gactgtctca ggattcccat catttcctac
     1021 ttggctatga ggatgcagct aatcatgtct tacttggata tgtccacgct gaagtttacg
     1081 aatcactcta ttccaaagca ggatttaata tcttagcttt agcagtttca cctcaagcgc
     1141 aaggtcaagg tattggtaaa agtttactac aagggttgga agaagaagca aaaagatgtg
     1201 gttatgggtt tatccgctta aattctgcca atcatcgtct gggtgctcat gcattttatg
     1261 aaaaagttgg ctatacttgt gataaaatgc agaaacggtt tattcgcatc ttttagtttg
     1321 attttcttat tgtaaaatca aactaatgga ctagtcacac aataaaggag aagacctatg
     1381 atttttgaca aagatgattt taaagcatat gatgctgatc tctggaatgc tattgccaaa
     1441 gaagaagaac gccaacaaaa taatatcgag ttaattgctt cggaaaacgt agtttccaag
     1501 gctgttatgg cagctcaagg gtctatcttg acaaataaat atgccgaggg ttacccagga
     1561 cgccgttatt atggtggaac tgatgtagta gacgttgtag agactcttgc tattgaacgc
     1621 gcaaaagaaa ttttcggtgc taaatttgcc aatgtccaac cacattcagg aagccaagct
     1681 aactgtgcgg cttacatgtc cttgattgag ccaggtgata cggttatggg aatggatttg
     1741 gcatcaggtg gtcatttgac tcatggggct cctgttagct tctctggtca aacctacaac
     1801 tttgtttctt atagtgttga tcctgaaacg gaactcttag actttgatgc tatcttgaaa
     1861 caagcccaag aagtaaaacc aaaactgatt gtagctggtg cttcagcata ttctcaaatt
     1921 atcgattttt caaaattccg tgaaatcgca gatgctgtcg gtgcgaagct catggtggac
     1981 atggcccata tcgctggttt ggttgcggct ggccttcatc caagcccagt tccatacgct
     2041 catatcacaa caacaacgac ccacaaaacc cttcgtggac ctcgtggtgg tttgattttg
     2101 accaatgacg aagaacttgc taaaaaaatc aattcagcta ttttcccagg tattcagggc
     2161 ggtcctttag agcatgttgt ggcggctaag gcagtttcct tcaaagaagt tttggatcca
     2221 gccttcaagg aatatgctgc caatgtaatt aagaacagca aggctatggc agatgtcttc
     2281 ttgcaagacc ctgatttccg tattatttca ggtggaactg aaaaccatct cttccttgtt
     2341 gatgtgacta aggttgtaga aaacggaaaa gttgctcaaa acttgctgga tgaagtcaat
     2401 attaccctaa ataaaaattc aatcccttac gaaaccttgt cgccattcaa gacaagtggg
     2461 attcgtatcg gagcagcagc tattactgca cgtggatttg gtgaagaaga aagtcgcaaa
     2521 gtggctgaac tcatcattaa aacccttaag aattcagaaa atgaggctgt attagaagaa
     2581 gtgagaagtg cagtcaaaga attgacagat gccttcccat tatacgagga ctaaaacttt
     2641 tatggatatt tatattaaga aagccattat tcaccagttc agtccggatg ataccgagct
     2701 gttcttagca gataagtttc tcaatattac tccaaaaatc gaagaatacc tacgtaaaaa
     2761 aattgaacat gtgtattcag atgaagccaa gactgggatt ttcgaagaag aaaatccctt
     2821 cttcaatcat attacagacg atttgttgga gacatcagta acgctggcta atctctggaa
     2881 agaggagttt agcatttctg aaaatctcaa gaccaatgac ttgatttttg ttcaattttc
     2941 taaagaaggt gtagaacatt tcgctttctt gcgaattgcc ctgcgggaga ccttgaccca
     3001 cctcggagga gaagttgata atccaatcaa gctgactcag aataacctgc ctggatttgg
     3061 aacgggtgct gacgaggcct tggtggtcaa tcttcagagt cgcaagtatc acctgattga
     3121 aaaacgaatc aagtacaacg ggactttttt gaactatttt tcagataatc ttcttgctgt
     3181 cgctcctaag atttctccta aaaaatctat caaggaactg gaaaaaacag cccagagaat
     3241 tgctgaatct tttaacacag atgattttca atttcaatcc aaggtcaaat cagctatttt
     3301 caacaaccta gaagaaagca atgaattgtc acctgagaaa ttggctaatg acctttttga
     3361 caacaatctg acggctcgtt tgagctttat tgaccaagtc aaagaagccg taccagaacc
     3421 tgttcaattt gatgaaattg atgccagtcg ccaattaaag aaatttgaaa accaaaaact
     3481 ctccttatca aatggaattg agctcatcgt tcccaataac gtctatcaag acgccgagtc
     3541 tgttgagttt atccaaaacg aaaatggaac ctactctatc ttaatcaaaa atatcgagga
     3601 tatccaaagt aaataatgtt taaacgaatt cgaagagtgc ttgtactagc agtcttcctt
     3661 tttgctggct ataaagctta ccgcgttcat caagatgtca aacaagtcat gacctatcaa
     3721 cccatggtgc gagaaatatt gagtgaaaaa gacaccccag caaacgaaga gcttgtgctt
     3781 gctatgattt atactgaaac aaaaggaaaa gaaggcgatg ttatgcagtc tagtgagtct
     3841 gcaagtggtt ccaccaacac catcaatgat aatgcctcta gcattcggca aggcattcaa
     3901 actctgacag gcaatctcta tctggcgcag aagaagggtg tagatatctg gacagctgtt
     3961 caagcctata attttggacc tgcctatatc gattttatcg cccaaaatgg caaggaaaat
     4021 accttggctc tagccaaaca gtactctcgt gagactgttg cccccttgct tggtaatagg
     4081 actggaaaga cttatagtta tattcacccc atttccattt ttcacggtgc tgaactctat
     4141 gtaaatggag gaaactatta ttattctaga caggtacgac ttaaccttta catcatcaaa
     4201 tgtttcactc tcttttcaac atctggctag accaggtgtt tttgttataa gttttcttta
     4261 agatagatat attactctag aaaagtaaag gaggaaattc cctatgagaa agaaactctt
     4321 tctgaccggt gctgcggtct tgtgggcagt aacagctatg aatagcgccc atgcagcaac
     4381 agatgttcaa aaagttatcg atgaaaccta tgtccaacct gaatatgtcc taggttcctc
     4441 cctatctgaa gaccaaaaaa atcaaactct taaaaaactg ggctacaatg cctcaacaga
     4501 taccaaagaa ctcaagacca tgacacctga tgtttattct aaaatcatga atgtggtcaa
     4561 tgactctagc ttacagttgt attcatcagc caagattcaa aagctaggtg acaaatcgcc
     4621 acttgaggtc aagattgaaa caccagaaaa tatcactaag gtgactcagg atatgtaccg
     4681 aaacgcagca gtaacgctgg gtatggaaca tgccaaaatc actgtagcag cccctattcc
     4741 agttacaggt gagagtgctt tggctgggat ctactattcg ctagaagcta atggagccaa
     4801 ggtgccgcaa gccaataaag atttggctca agaagagcta aaggctttgt cagatatcaa
     4861 tgctgaaaac aaggacaaat caggctatga tgctaataaa ttaaacgttg ccctagctga
     4921 tatcaagtca ggactcgcca aagctaaaga aagcaaggga aatctgacag aagaagatgt
     4981 acgcaagatt gttgaagata ccttaaaaaa ttacaaactt gatcaggtca taacaggaaa
     5041 ccagatcaat atcatcatca attttgcctt gaatctctca aagagtgata tcctcagcaa
     5101 tgcagatttc actaaaaccc taaatgacct taaacaaagc atcgtatcac aagctggcga
     5161 cagttttaaa aatatcaacc ttaactttga tgcggataag gcgctagagg acggtggtaa
     5221 cttcttaagc tccctctggc aagcccttgt caacttcttc aagagttttg gttcttaaga
     5281 aatttcatgg tataatggat ggtaaccgta acgttgccgt cttttttgtc ggccaataga
     5341 aagagaaaag aatgttaaag aaaaatgata ttgtagaagt tgaaattgtt gatttgaccc
     5401 atgaaggggc aggagttgcc aaggtagatg gtttggtctt ttttgtagag aatgctttac
     5461 cgagtgaaaa aattctcatg cgtgtcctca aggtcaataa aaagattggc tttggaaaag
     5521 ttgaaaaata ccttgtccag tcaccacacc gtaatcaaga tctagatttg gcttacctgc
     5581 gttcaggaat cgcggattta ggacaccttt cttatccaga acagctcaag tttaaaacca
     5641 agcaagtcaa ggacagtctc tacaagattg ctggaattgc agatgtagaa gttgctgaaa
     5701 cgcttggtat ggaacatcca gtcaagtatc gcaataaggc gcaggtgccc gttcgtcgag
     5761 tgaatggtgt cttggaaaca ggatttttcc gtaagaattc gcacaacctc atgccccttg
     5821 aagatttctt tatccaggat cctgtcattg accaagtcgt agtagctctt cgagacctgt
     5881 tccgtcgttt tgatttaaaa ccttatgacg aaaaggaaca ttctggattg attcggaatc
     5941 ttgtggtgcg tcgtggtcac tattcagggc aaatcatggt cgttttggtg acaactcgtc
     6001 caaaagtttt tcgtgttgac caattgattg aacaagttat caagcagttc ccagagattg
     6061 tgtctgtcat gcaaaatatc aacgaccaga ataccaatgc gatttttggt aaggagtggc
     6121 gcactcttta tggtcaagac tatattacgg accagatgtt gggaaatgac ttccaaatcg
     6181 ctggcccagc cttttaccaa gtcaatactg aaatggcgga gaaactctat caaacagcca
     6241 ttgactttgc agagttaaaa aaagatgacg tggttattga tgcttattct ggtattggaa
     6301 ccattggttt atcagtcgcc aagcatgtca aagaagtcta cggtgttgaa ctgattccag
     6361 aagcggttga gaatagtaaa aaaaatgctc agctgaacaa tatttcaaac gcccactatg
     6421 tctgtgacac agctgaaaat gctatgaaga attggcttaa agatgggatt caaccaaccg
     6481 ttatcttggt tgatcctcca cgcaagggct tgacagaaag ctttatcaaa gcaagcgccc
     6541 aaacaggagc cgatcgcatc gcctatatct cctgcaatgt cgcaaccatg gcgcgtgata
     6601 ttaaactata ccaagagttg ggatatgaat tgaagaaagt ccagccggtg gatctatttc
     6661 ctcaaacgca tcacgtcgag acggtagcac ttttgtccaa actcgatgtc gataagcaca
     6721 taagtgttga aattgagctg gatgagatgg atttgacaag tgcggagagc aaagcaacat
     6781 atgctcaaat caaagaatat gtttggaata aatttgaatt aaaagtttcg acattatata
     6841 ttgcacagat aaaaaagaaa tgtggaatag aattacgaga acattacaac aagtctaaaa
     6901 aggataaaca aattattcca cagtgtacac ctgaaaaaga agaagccatc atggatgctt
     6961 tgagacactt caaaatgatt taatagaaaa gaatgacagt atatgacttt ctgcatttat
     7021 tacattccta cttggtatag gaacagctat tattcctttc ttgcaaggta tcaattagaa
     7081 aataggctca atataaagat tgataggatc atttttatat ttaaaggagc gttgaaatga
     7141 ttgataaagg caacaaaaaa ttttaggata aatttgctaa gttgtatgcc ccttttatga
     7201 aaaaagataa agaggtttat gataaagttt gtgaatatct tagtcctcat ttgaataaag
     7261 atatggaggt gcttgaactt gcttgttggt ttcgtgtcat aacagttata gaggcaaata
     7321 gttatgtaaa tataaggagt tcaagacttc taccaaagtt taaaactcaa aaaataaat
//
LOCUS       DAVLSL010000093         7173 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLSL010000093 DAVLSL010000000
VERSION     DAVLSL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.095"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(301..1149)
                     /gene="dprA"
                     /locus_tag="VXN30_001508"
     CDS             complement(301..1149)
                     /gene="dprA"
                     /locus_tag="VXN30_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1358114.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1245..1934)
                     /locus_tag="VXN30_001509"
     CDS             complement(1245..1934)
                     /locus_tag="VXN30_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1358115.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1946..2824)
                     /locus_tag="VXN30_001510"
     CDS             complement(1946..2824)
                     /locus_tag="VXN30_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1358116.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2814..3683)
                     /gene="licA"
                     /locus_tag="VXN30_001511"
     CDS             complement(2814..3683)
                     /gene="licA"
                     /locus_tag="VXN30_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1358117.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3700..4722)
                     /locus_tag="VXN30_001512"
     CDS             complement(3700..4722)
                     /locus_tag="VXN30_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1358118.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4727..5434)
                     /locus_tag="VXN30_001513"
     CDS             complement(4727..5434)
                     /locus_tag="VXN30_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1358119.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5770..>7173
                     /gene="tacF"
                     /locus_tag="VXN30_001514"
     CDS             5770..>7173
                     /gene="tacF"
                     /locus_tag="VXN30_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1358120.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYIS"
BASE COUNT         2199 a         1490 c         1211 g         2273 t
ORIGIN      
        1 atcaaagcgc aaactaggaa gctagccaca ggttgctcaa agcactgctt tgaggttgta
       61 gataagactg acgaagtcag ctcaaaatac tgttttgagg ttgcagatgg aagctgacgt
      121 agtttgaaga gattttcgaa gagtattata tgagaaaaat cctctactct actaatccac
      181 ttagaaaaaa cgcatattat caagtgttaa aaacttgata atatgcgttt tattatggaa
      241 atgttgggtt cattttctct taaaattgat tttttcttag aattttagct taggtcattt
      301 ttaaaattca aattccgcaa gaacatcttg cccactggtg accaattttg ctccttcttg
      361 aatcaaatga tggcaaccgt ctgatagtcc atctaaaatg ctaccaggaa tagcaaagac
      421 atcgcgtcct tcttccattg ctcgctcaca cgtaatgaga ctacctgaac gcatcttagc
      481 ctctgctaca atcacaccac gacaaagtcc agcaatgatg cgattacggg caggaaaatg
      541 aaatttcaga ggttgttcgc caggtccata ttcacttaga accagatgat cattgccgat
      601 gtagtcttgc aagcgtttat tggctttagg ataaaacaca tccagtcctg ttccaatcac
      661 tgcaatggtt tttccgccat tctgaagagc tgccatatga gctgctgtgt caatgccctt
      721 ggccagacca ctgacaataa ccagttcatt ttccaagcct tgaatgactt tttcaactga
      781 cttagctccc tgtttgctac aagcacgact gcccacgacc gctaccttcg ggaatttcag
      841 gaggtcaaga tttcccttgt aaaataaaag tacaggcgca tcatatattt cactcaaatc
      901 ccaaggataa cagtcatcta aaatagagaa agatggaaat ttttgaaact ctttcgacaa
      961 atgcgcatcg tctatctgaa aataacgttc cataaaaacg gctggattac ggcaacctga
     1021 tatatctgca atatcaccca acaaaagctc ctgatcaaca ttttcaccgt attctagcac
     1081 tttcaaaatc tgttgattgg tcaaacctga tttttttaac ttatagattt catagtttgt
     1141 gattttcata aataactcca tttctttttc tactcatcta tctattcgta aaagttcgta
     1201 aaaaatcaaa aaacatccga ctattttgtc agatgttgga atctttaatt ttcgttttta
     1261 agaatttctt ctaatttacg atagtcttgg acactatcga tctcataaat gctattgcct
     1321 tctaattctt caacatagac atctagctct ttgatattat ccttaaccat attgtcccaa
     1381 tagagatcaa caaattcacc acttgcataa gccttgtcga taaagctgac aatcttttct
     1441 gcagttggag catcccagaa ggatacacca ctaaggatgc gacctgcctt gctatcaaca
     1501 ataatgtctt gaaccttgta gtcatctcca tagaccaaga accattcgtt ggtacaatct
     1561 tcacgataaa cactaaaata agtcgaacgt gtcaaatcat tgcggaacat atttttaaag
     1621 agataattgt cagcatcaat aacatagctg ttggccaatt cttcttttac aagatagaga
     1681 gagtaaaagt tattgtagtc agcgtattta tcattgaaaa cgaggcgaac accgtatttt
     1741 tcttttaagt aatcgaattg ttctttaaga taaccaacaa tgatgatgat gtcattgatt
     1801 cctttttctt tgagaaactc aatttggtat tcaatcaaag gtttttgatt aacctgaacc
     1861 aaggctttag gggtattttc agtcatagga cgcaagcgag ttcccaatcc cgctgctaag
     1921 ataatggctt tcacacgaat ctcctttatt ctttaataat aatataaact ccagcaatga
     1981 cgacaagtga cgtcataatg gtcagcatat ctagcggtgc acccaaaaaa acaactgcaa
     2041 acaagaccgt ccatactaca tagctcacgt tcaagcctgt agccttggct ggttgcaagc
     2101 gattgatagc gatataataa gccaagtagg aaatcatatc aaaggctgca aaaacaatca
     2161 tgagacctag caattgtcca ttggctactg cagtaaatga ctgatgagag aagagcacaa
     2221 tcacaagata ggacaagaac gaagtcactt gacggattaa gagggcttcg atttcactca
     2281 gttcactttc catagcaaag gagctaagaa cactctcact tccccatgca atagcacaaa
     2341 ccaaagcaca gagaatccca atgtagaagg aattaacctg ttcaacctta taggtctggg
     2401 caataatccc tccaataatc aagacaatcc caaatacagt atttttcgaa atcttgtgct
     2461 tcaaaaagaa aaaagccaat agaactgaaa tcgcagggta aatagccgat acagatgaag
     2521 ctaaagaact tccgatatac ttaactgcat aaagattggc ctgcataccg atagggcctg
     2581 ctagcaaggc tccgatgata acactgacat tgcgaatatt taagaaaatt gagaggcgaa
     2641 ctttcccttc ttttaccaag agaaaagcta gtaagataaa gatgctcaaa aaatcatgag
     2701 ctgcagccac cacaaagggt gacaaatctg taaaaatcga aaagatataa gcactaaccg
     2761 ttagacccaa gccccagaaa atacctgaga gaaggccaaa aggaactccg tttttacttt
     2821 tcatctgaac ctccataaga agccaaacct ttaatagctc tttggtaacg attcacacca
     2881 tagtcaccaa aatcttcacc ttgctcttcc ttatagacag tccatagact ccaaatagta
     2941 tcttgtaaaa ttttataaat agcaatcttt tcatgagaaa ccggtgtttg gtcactctca
     3001 tagtgagata agaaagtttc ttcctcttgg gaagtgaatt cagactctaa aaagagggca
     3061 gccaaatccc acattggatc attcattgat gaatattccc agtcaatcaa ataaagtcgt
     3121 ccttgaggtg attcgataaa gttttcaggc accaaatcga tatgacaaga ttttctgtca
     3181 acacctaagt cagccagtct tttctctaag gagaagactg catttctaac agattcatag
     3241 ttggcataag gaatttgttc ttcaatcaag gattcgtatt ttttgatttc ttcaaaagga
     3301 gcaaattctc ctcttaattc cttagcagac gtatgaatag tttgtaatat tggagtaatt
     3361 ttgtcgaact tggtcttgat tgacgttgaa tcaagcgtaa tcgcagattc gatatactca
     3421 tttactttga taccagcttc aatatcaaaa agataatttt ttacatctaa gcctaaatcc
     3481 tttagtagtt caagattgta cttttcatct tgtcgattga taagcttttc tgtcccttta
     3541 ccaaagaatt taacaatgta ttgcttattt gttgttttgg ccaaatagtt ttgattggtc
     3601 attccaccca gttgttcaac actgaggact tcctcttctt gactaagtaa ggaagaaatt
     3661 ttttctttaa tgattttctc cacaattaac ctccagtact tatacttccc acttaaacac
     3721 tgttttaaag gctgtgttta aatcggttgc aaagacacga tgaatatctt taatttctct
     3781 tacaggttct tctagataaa ggatattttt aagacgatta gcaaatttct tgacttccat
     3841 catttggata gcattttcaa aatcaatgcg accagaacga gatgacccaa ccaaaatcaa
     3901 gcccttttct aaggcatcgc gagtattgag attgacttta tattcgctaa ctcccatcat
     3961 gagaatcgtt ccctgaggac gaatgtagcg aatcaagtca ttaatagctg gtccagtacc
     4021 atcaccacca caacattcaa aagcatggtc aaaggccaaa tcttcaggaa tattatccgt
     4081 aatatagcat tctttggcaa atgagaagag ttccaacttt tcccaatgac gaccaataac
     4141 cacaatctct gcttctggca aagtatagtt gataatattg gcaaccacaa aagctaaact
     4201 tccatctcca ataacggcga tccgctcccg cttgctatga gcaagagtca atagacgatt
     4261 catagcgtgc atgcccacac tgacaaactc tgtaatggct gcaaccgtat cttcaatagc
     4321 atcataagcc accacacgat ctttagggag agaaacaaac tctctcataa agccatcaaa
     4381 tccactagac aagaaatggg tccctgtcat gtagttttca tagaattctt catcactctg
     4441 cataggagac tgattgggaa tcatgacaac tttttgacca acctcgtagg ttccggtcgg
     4501 gtcagaaatg acggttccac atgactcgtg aatcattgcc attggaagct ttttattcaa
     4561 aatcttggga tcacgttttc cctgatagta acgctgatcc gcatgacaga cagccatgta
     4621 gttgggacgg ataaggatat gattctcttg gtcaatagcc tcttcctgat atttgacatt
     4681 gataaactta ggcttagtta gttgataaat ttgattaatc attttactag tctttctcaa
     4741 tcatactttt tgcaatcttc aaatctgtta cggttgtaat cttcagattt gagtattcac
     4801 ctttggccaa agccacatct tttcctttga tcacaaagat tttacatgca tctgtcaaga
     4861 tttccttctc ttcatcagaa agagatccat aaaggtccat gaagtccttg caacggaatg
     4921 tttgaggtgt ttgtccttga taaaggtgag cacgatttgg aatatctgta ataaattgac
     4981 cattggtact ttcaacgata gtatcaaccg cttctaccac tgtgtccact gcgtcatgat
     5041 tttgggcaag ttggatattg tcctgaatca tgcgaagtgt aataaatgga cgaacagaat
     5101 cgtgggtaac aacgatatcc tctggagtaa gcggacgata agcatcaatg gcttcaatga
     5161 tgttcttaat acttgtattg cggtcagcac caccctttgt aatgatgata cgttccttat
     5221 aaagaggaag atatttatct acaagatctt ctgcatgaga aacccagtct ccatgaacac
     5281 caactacaat tttttcaata cttggctcca agacaaattt ttcaattgta tgaatcaaaa
     5341 taggtcgatc acctagctct aaaaattgtt ttggcaagtt actgatcccc atgcgtgtgc
     5401 cagttccacc ggcaagaatt cctgcataaa tcatctattt tctcctttgt cttactaaaa
     5461 aaacttattc tctctattat accacaatct agtcctatca taaaagaaat actggtcctc
     5521 cccttctaga ataggagaat tatgcagttc tattattcaa agaaactagt ccatttctag
     5581 taatgactag gaattctgta gcaattacta ataaatggct gcgaaatttt agagtccttc
     5641 agaataatat actttcctta aagaaaactt aaaacttaag atttgaaact tcaaagtgat
     5701 tacttgactt actgcccttc atttcttatt agctaacttt atgatataat gaaaaacgag
     5761 gtaaattgaa tgaaaagtat aaaattaaat gctctatctt acatgggaat tcgtgtcttg
     5821 aatattattt ttcccatcct aactggaacc tatgtcgcgc gtgtcttgga ccgaactgac
     5881 tatggttact tcaactcagt cgacactatt ttgtcatttt tcttgccctt tgcaacttat
     5941 ggtgtctata actacggttt aagggctatc agtaatgtca aggataacaa aaaagatctt
     6001 aacagaacct tttctagtct tttttatttg tgcatcgctt gtacgatttt gaccactgct
     6061 gtctatatcc tagcctatcc tctcttcttt actgataatc caatcatcaa aaaggtctac
     6121 cttgttatgg ggattcaact cattgcccag attttttcaa tcgaatgggt caatgaagct
     6181 ctggaaaatt acagttttct cttttacaaa actgccttca tccgtatcct gatgctggtc
     6241 tctattttct tatttgttaa aaatgaacac gatattgttg tctatacact tgtgatgagt
     6301 ttatcgacgc tgattaacta cctgattagt tatttttgga ttaaaagaga catcaaactt
     6361 gttaagattc acctaagtga ttttaaacca ctctttctcc ctctgacagc catgttagtc
     6421 tttgccaatg ccaatatgct cttcactttt ttagatcgcc tcttcctcgt taaaacaggg
     6481 attgatgtca acgttagtta ctataccata gctcagcgaa ttgtgaccgt tatagctggg
     6541 gttgtaacag gtgcaattgg agtgagtgtg cctcgtctca gttactatct ggggaaagga
     6601 gacaaagaag cctatgtttc tctggttaat agaggtagtc gaatctttaa cttctttatc
     6661 attccactga gttttggact catggtttta ggaccaaatg ccatcctact ttacggtagt
     6721 gaaaaatata tcggaggcgg catcttgacc tctctcttcg cttttcgtac gattatcctg
     6781 gccttagata ccattcttgg ttcccaaatt ctctttacca atggctatga aaaacgtatc
     6841 acagtttata cagtctttgc tgggctactc aatttgggct tgaatagtct cctctttttc
     6901 aaccgtatcg tggctcccga atactactta ctgacaacta tgttatcaga ggcttctcta
     6961 cttgttttct atatcatttt catccataga aaacaactca tccacttggg acatatcttt
     7021 agctatactg ttcgatactc tctcttttca ctttcctttg tagcaattta tttcctgatt
     7081 aatttcgtgt atcctgtgga tatggtcatt aatttgccat ttttgattaa tactggtttg
     7141 attatcttgc tatcagctat ctcttatatt agt
//
LOCUS       DAVLSL010000094         7120 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLSL010000094 DAVLSL010000000
VERSION     DAVLSL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.096"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(103..2100)
                     /gene="metG"
                     /locus_tag="VXN30_001515"
     CDS             complement(103..2100)
                     /gene="metG"
                     /locus_tag="VXN30_001515"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1358121.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPEGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHAHMEAVDYPRALEAVWTLISRTNKYIDETAPWILAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2224..3483)
                     /locus_tag="VXN30_001516"
     CDS             complement(2224..3483)
                     /locus_tag="VXN30_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358122.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3485..4186)
                     /locus_tag="VXN30_001517"
     CDS             complement(3485..4186)
                     /locus_tag="VXN30_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358123.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4170..5369)
                     /locus_tag="VXN30_001518"
     CDS             complement(4170..5369)
                     /locus_tag="VXN30_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1358124.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5634..6980
                     /gene="gor"
                     /locus_tag="VXN30_001519"
     CDS             5634..6980
                     /gene="gor"
                     /locus_tag="VXN30_001519"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1358125.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         2071 a         1576 c         1347 g         2126 t
ORIGIN      
        1 cagtgctttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
       61 aaataaaaaa acagaactag tctgaactag tcctgtctac ttttacccaa tcacacttcc
      121 atttggtaca gctggatcaa ctgtgagaag ggttaatttg ccatcatgtt cagctgatag
      181 aatcataccc tggctgacat attttttcat cattttacgt ggtttgaggt tagcaacgat
      241 ttgaactttc ttgccgacca attcttgttc atttggatag tattttgcaa ttcctgaaag
      301 aatctgacga tcttctccat caccagcatc caagcggaat tgaagcaact tatctgaacc
      361 ttctacttta gacacttctt tgacttctgc cacacggatt tcaaccttgt caaagtcttc
      421 aaacttgatt tcatccttgt ttagtttgag ctcaacttcg tccggattcc attctttttc
      481 gactgctggt ttattgcctt ccatttgttc cttgatatag gcgatttctt cttccatatt
      541 tagacgtgga aagataggtg ttcctttggc aactacagtc acatctgctg ggaagtcagc
      601 caaactcaag ttttcaagac tagaaacttc ttccaaacca agttgagtca aaactgcacg
      661 actagtttcc atcataaatg gttcaatcaa gtgagcaact acacgaatgc tggctgccaa
      721 gtggctcatg acacttgcca attggtcacg aagagcttca tccttggcca agatccatgg
      781 tgcggtctca tcgatgtatt tattggtacg agagatcaga gtccagactg cttcaagcgc
      841 acgtggatag tcaactgctt ccatgtgtgc atggaagtct gcgattgatt gttctgcaac
      901 ctcagcaaga acatggtcaa attcagtcac accttctaca taggcaggga tttgtccatc
      961 aaagtactta ttaatcatgg aaaccgtacg gttaaggagg ttcccaaggt cattagccaa
     1021 ttcatagttg atacggccga catagtcttc aggagtaaag gttccgtctg aaccaactgg
     1081 aaggttacgc atgaggtagt aacgaagtgg atctagtcca taacgctcta ccaacatttc
     1141 agggtaaacg acattccctt ttgacttaga catttttccg tctttcatga caaaccaacc
     1201 atgggcaatc aaacgatcag gtaatttaac atccaacatc ataagaagga ttggccagta
     1261 gatagagtgg aagcgaagga tgtcttttcc taccatatgg aagactgttc cattccagaa
     1321 cttgtcaaag ttaccatgtt cgtcttgagc gtagccaaga gctgtcgcat agttaagaag
     1381 ggcatcaatc caaacgtaga caacgtgttt tggatttgat gggacaggca ctccccatgt
     1441 aaaggttgta cgagataccg ccaaatcttc caaacctggc tcgatgaagt tgcgtagcat
     1501 ttcattaagg cgaccctctg gcgtgataaa ttcaggatga gctttgaaaa attcgaccaa
     1561 acggtcttgg tatttgctaa ggcgaaggaa gtatgattct tcagaaaccc attcaacctc
     1621 atgacctgat ggagcaatac caccagtcac atttccagct tcatcacgga aaacttctgc
     1681 cagctggctt tctgtaaaga attcttcgtc tgatactgaa taccaaccag agtattcacc
     1741 caagtagata tcatcttgag caagtaagcg ttcaaagacc tgtgcaacaa ctttttcatg
     1801 gtagtcatca gttgtacgga taaatttatc gtatgagata tctagtaatt gccagagttc
     1861 tttaactcca accgccattc catcaacata ggcttgaggt gtaataccag cttcttccgc
     1921 tttctgctgg attttctgac catgttcatc aagacctgtc agataaaata catcgtagcc
     1981 catcaggcgt ttgtaacgtg ctaggacatc acatgcgata gttgtgtagg cagaaccgat
     2041 atgaagtttc ccagatggat agtaaatcgg cgttgtaata taaaaatttt tttcagacat
     2101 aatttttcct ttccaggcaa atgaaacctg tttttctaac acttcattat atcacatttt
     2161 taatgaattt cgatagggaa atccatacaa aaacaagata gacgagtgtc catcttgttg
     2221 atctcattca taacgaaggg cttcaattgg atcaagtttc gatgccttgt tggctggcaa
     2281 gactccaaaa atcataccaa cactagccga aactgcaaga ctaaataggg caactgggat
     2341 tgatactcca acttctatac cttctattaa accttgcagt aacaaacctg ctaaggcagt
     2401 taaaccactt gcaattgtca agccaattaa gccacctaac aaggtcaaaa tcatggattc
     2461 aatcaaaaac tgaattaaaa tattggcacg tgttgcgccc aaagccttac gaagaccaat
     2521 ctcacgagtg cgctctgtca ccgaaaccag catgatgttc atgacaccag ttcctccaac
     2581 aaagagagaa atccctgcga tggaactaat aatcgtcgtc ataaaactaa acgattgttg
     2641 aatttctgca aatacaacgg actcatctgc cacctggtat tctccctgtt gtaagcctgc
     2701 aagctctgtc atttttcgtg ccagttctgg acccagagtt ggggttaaac tggtatcatt
     2761 cactcgaaag acaatactag ctatttcatc tatattaaaa ttcgcagcaa gggagatatt
     2821 ggtagtaata ggcaagccac caaacccata tatttttgac cttttagcct ccggactagt
     2881 ataaacccca atgacccggt aactaaatcc attgacttct acaaccttgt taatagcctc
     2941 ttgaggagat tcaaataaac taatggacaa ttcctcatct agcaaaataa cacttgcaaa
     3001 ctctttgaaa tcttgctctc tcagactacg acctgcaata atttcattct taacagcgtc
     3061 catgtaagtt ctgtttccac ctgtcaaatt agcattctca acctttttat cttgataggt
     3121 caagatggca ttcgttgaat tggttacata gtaactatcc actcccttca gtttagctgc
     3181 ctcttggacc caggattctt gcggttttgg cggttcaaca ggaatttcct cttcctttcc
     3241 agaaaccgta aaagatgatt gtttctgagt aaaagaacca tctttacttt ttttaggaga
     3301 gaaaaagacg ctaatatttt tctgagattt agtcatatct ttattgactt gacgagatag
     3361 ggaatcaccc aaagccataa tcacaacaac tgatgaaaca ccgataataa tcccaatcat
     3421 agtaagcaaa gaacgcatct tgtgagccat gatagatgaa aaggcaaatt tcagattctg
     3481 catcttagtt ttcctccttt cctaactgag cactgtcaga cgaaatgacc ccatcccgaa
     3541 tgacaatctg acgtttagca taggcagcaa tctcaggctc atgcgttacc atgataatgg
     3601 tttttccttc tttattcaaa tcaaccaata attgcataat ttggttacct gttttggtat
     3661 ccaaggctcc tgtcggttca tccgctagga taatagaagg attgtttacc aaggcacgcg
     3721 caatggctac acgttgcttt tgaccaccag ataattctga aggtaaatgg tgactacgtt
     3781 ctgtcaattc aaccttgtct aaatattcct cagccaactt gcgacgtttt gaagacgaaa
     3841 ctcctgcgta aatcaagggc aattctacat tttgcagagc attgagcttc gatagaagaa
     3901 agaactgctg aaagacaaaa ccgatttgtt ggttacggac cttagccagt tgtttttcac
     3961 caagcccagc cacttcttga ccttcaagat aatattctcc actggttggt gtatccaaca
     4021 tgccaatcgt attcatcaga gtggacttac cagacccaga tggtcccatg atggctacaa
     4081 attcaccctc attcacttct agattgatat ttttgagaac ctgcagttct tggtcaccat
     4141 tacggtagct tctgaagata ttttttagac taattagttg cttcatcagc cttcacctct
     4201 tttccttctt ccaaggaaga tgttggatta ctgatgacct tagcaccatt cgttaaacca
     4261 gaagtgattt cttgattttc tgcgtcagca tttcccaatg aaacctcaac ttttttagcc
     4321 ttttgttgtt catccacaat ccagacataa tttttactat catccattac tagactgcta
     4381 acaggaacaa gaatagcctt agttttgctt ttaacctcaa tgttgacaga aaaaccctgt
     4441 ttcaaatcac caacctcgcc tgtcacatca atagtataag ggtatttaga acctgtatta
     4501 ttcccggctg ctggactagc tgcttcacca ttgtttttag gatagtcaga aatatagctt
     4561 aatttcccag tccatttttt atcaggatac actttagaag taaagcttac ttcttgacct
     4621 acagaaaggt tggctagatt gtactcagac aattctccct tgacttgtaa attttcattg
     4681 ctgacaatat gaaccataac ttggctcgcc cctgttggag atttagaaac attgctattg
     4741 acttcgacta cagttccctc tagggtactg agaacagttg ttgcatccaa ttgactttga
     4801 gccttgctta attgcgctgc agcatctgca cgcgcatcac gggcatcacc caattgagcg
     4861 tcaatagaag caacagaatt tccagccact ggagttgggc tttgcaccgt tgcatcttct
     4921 cctcctactg gcgctggtaa ctgtggagcc ggagctgaag cggcttcatt tcgtgcttga
     4981 ttgagttcat tgatatgacg atctgcctta gctactgctc gactagctga atcataggcc
     5041 gcctgcgctt ctgaactact gtacttgact aaagcctgcc cttcgctgac cttatcgccc
     5101 acagaaacaa ggatttcatc taaatcaccc ttactagcat caaaataaac atattgttca
     5161 ttttttgctg ttactgtccc tgacaataaa acagaggagg ccacgcttcc ttccttggca
     5221 acaacaagat gagtaggctc atcttttaga gcagtctgag aaggttgtct aaagagtaaa
     5281 atccccccag cacccaatac aactacactc gcagcaccga ttgctgcata cagttgccac
     5341 tttttagctt taccattctt tttcttcata atgaaactcc ttttcttttt tacaatactt
     5401 tgctattata ccaaatttcc ctccagcaaa caatacagtt caggattaaa caatcgttcg
     5461 gaattttgct tttctattgt actagatata taatacacta tcttgactta tttggcaagc
     5521 ttttttgcaa ttttttgtgc gtagcagatt tttgcaatca aatcaaaaaa agaataaaat
     5581 ataactatca aaaaagacgc tctactattt aaaagattac aaaggagttt tcaatgagag
     5641 aatatgatat cattgctatc ggtggaggta gcggaggaat cgctaccatg aaccgtgctg
     5701 gtgaacatgg agccaaagca gccgttattg aggaaaagaa attaggtgga acctgtgtca
     5761 acgtcggttg tgttcctaaa aaaatcatgt ggtacggggc gcaaatcgct gagactttcc
     5821 atcaatttgg agaagactac ggctttaaga ctactgatct taactttgac tttgcaaccc
     5881 tacgtcgcaa tcgtgaagcc tacattgatc gcgctcgttc ttcttatgat ggtagtttta
     5941 aacgcaacgg tgtagacttg attgaaggtc atgctgaatt tgtagattct catactgtaa
     6001 gcgtaaatgg tgaactgatt cgtgctaaac atatcgtgat tgctacaggt gcccatccaa
     6061 gtattcctaa tattcctggt gctgagctag gtggctcttc tgatgatgta tttgcctggg
     6121 aagaacttcc agagtcaatt gccattctag gcgctggtta tatcgccgtt gaattagctg
     6181 gcgtactcca cacttttggt gtcaagacag atctctttgt tcgccgcgat cgtcctttac
     6241 gtggttttga ttcctacatc gttgaaggtt tggtcaagga aatggaaaga acaaacttac
     6301 cacttcacac tcacaaagtc cctgtcaagt tagaaaaaac tactgacggc attaccattc
     6361 atttcgaaga tggtactagt cacacagcta gccaagttat ctgggctaca ggtcgccgtc
     6421 caaacgttaa gggcttgcaa cttgaaaaag ctggagtgac tctgaacgaa cgtggcttta
     6481 tccaagtgga tgaataccaa aatactgttg ttgagggaat ctatgctcta ggtgatgtaa
     6541 cgggcgagaa agaactgact ccagttgcaa tcaaggccgg acgtacccta tctgaacgtc
     6601 tctttaacgg aaaaactact gcaaaaatgg attactcaac tattccaact gttgtctttt
     6661 cacaccctgc tatcggaact gttggtttga cagaagagca agctattaaa gaatacggtc
     6721 aagaccaaat caaggtttat aaatcaagct ttgcatctat gtactctgct tgcactcgca
     6781 accgtcaaga atcccgtttc aaactcataa cagctggttc agaagaaaaa gttgtcggac
     6841 ttcatggaat tggctacggc gttgatgaaa tgattcaagg atttgccgtt gctatcaaaa
     6901 tgggagcaac caaggctgac tttgatgcaa ctgtggcaat tcacccaact gcatctgaag
     6961 aatttgtaac catgcgttaa tcgaaacaaa agaagcaaag caaaaaacac ttctaaatat
     7021 caaaaagcga gtatttccgt agttggagat actcgttttc ttatgtttta ttttatagta
     7081 tgttgaagta agatatgcaa aaatcaatta acaaaccata
//
LOCUS       DAVLSL010000095         7096 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLSL010000095 DAVLSL010000000
VERSION     DAVLSL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.097"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            57..926
                     /gene="rfbA"
                     /locus_tag="VXN30_001520"
     CDS             57..926
                     /gene="rfbA"
                     /locus_tag="VXN30_001520"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1358126.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEHPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            927..1523
                     /locus_tag="VXN30_001521"
     CDS             927..1523
                     /locus_tag="VXN30_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1358127.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPASFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            1533..2582
                     /gene="rfbB"
                     /locus_tag="VXN30_001522"
     CDS             1533..2582
                     /gene="rfbB"
                     /locus_tag="VXN30_001522"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1358128.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGSRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            2648..3499
                     /gene="rfbD"
                     /locus_tag="VXN30_001523"
     CDS             2648..3499
                     /gene="rfbD"
                     /locus_tag="VXN30_001523"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1358129.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAKMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            3562..4645
                     /gene="glf"
                     /locus_tag="VXN30_001524"
                     /pseudo
     CDS             3562..4645
                     /gene="glf"
                     /locus_tag="VXN30_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476416.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4966..6948
                     /locus_tag="VXN30_001525"
     CDS             4966..6948
                     /locus_tag="VXN30_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1358130.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         2304 a         1221 c         1569 g         2002 t
ORIGIN      
        1 tcaaactaag taaaacataa ctcatgaaaa caaattaaaa aaggagggac tatagtatga
       61 aaggtattat tctagcaggt ggttcgggga cacgtttata tcctttgact cgcgctgcat
      121 caaaacaact tatgccggtt tatgataaac cgatgattta ctacccactt tcaacattga
      181 tgttggctgg gattagggat attttgatta tttccactcc acaggattta catcgattcc
      241 aagagcttct tcaagacgga tctgagtttg ggctcaaact ttcttatgca gagcaaccaa
      301 gtccagatgg tttggcacaa gcctttatca ttggggaaga gtttatttct gatgatagcg
      361 ttgcgctgat cttaggtgac aatatctatc acggttctgg gctttccaag atgctacaaa
      421 aggcagcgag taaggagtcg ggagcaactg tttttggcta ccatgtcaag gatccagagc
      481 gctttggtgt ggttgagttt gatcaggata tgaaggctat ttctattgaa gaaaagccag
      541 agcaccctcg ttcaaactat gcagttacag gtctctattt ctatgataat gatgtagtag
      601 agattgccaa gagtattaaa ccaagtcctc gtggtgaact ggaaattaca gatgtaaaca
      661 aggcttacct agatcgtggt gatttgtctg ttgagcttat gggacgtggc tttgcttggt
      721 tggatactgg cactcatgaa agtttactag aggcttcgca gtacatcgaa acagtccaac
      781 ggatgcaaaa tgttcaggta gcaaacttag aagaaattgc ttaccgtatg ggctatatca
      841 gtcgagaaga tgtattggcc ttagcccaac cacttaagaa aaatgaatac ggacagtatc
      901 tgctccgttt gattggagaa gcatagatga cagataattt tttcggtaag acgcttgcgg
      961 cacgcaaggt tgaagctatt ccaggcatgt tggagtttga tatccccgtt catggagata
     1021 atcgtggctg gtttaaagaa aatttccaaa aggaaaaaat gcttccactt ggatttccag
     1081 cgtctttctt tgcagaagga aaattgcaaa acaatgtatc cttctcacgt aaaaatgtcc
     1141 ttcgaggcct ccacgcagag ccttgggata agtacatctc tgtagcagat ggagggaaag
     1201 ttctgggttc ttgggttgat ctacgcgagg gtgaaacctt tgggaatacc tatcagacag
     1261 taattgatgc aagcaaggga atctttgttc ctcgaggcgt agctaatggc ttccaagttt
     1321 tatcagatac agtgtcatat agctatctgg tcaatgatta ctgggcgctt gaactcaaac
     1381 ccaagtatgc ctttgtgaac tacgctgatc caagccttgg tattgaatgg gaaaatattg
     1441 cagaagcaga ggtttcagaa gcagataaac atcatcccct acttaaggac gtgaagcctt
     1501 tgaaaaaaga agatttggaa taaggaaaga atatgactga atacaaaaat attatcgtga
     1561 caggtggagc tggctttatc ggttctaact ttgtccatta tgtttacgag aactttccag
     1621 atgttcacgt gacagtccta gataagttga cttatgctgg aaaccgcgcg aatattgagg
     1681 aaattttagg tagtcgtgtt gagttagttg ttggtgacat tgctgatgcg gagttggtag
     1741 acaagttggc tgctcaagca gatgctatcg ttcattatgc agcggaaagc cacaatgata
     1801 attcgctcaa tgatccatcg ccatttattc atactaactt cattggaacc tatactcttt
     1861 tagaagctgc tcgtaagtat gatattcgct tccaccatgt atcgacagat gaagtttatg
     1921 gggatctccc tttacgcgaa gatttgccag gtcatggaga agggccgggt gagaaattta
     1981 cggctgaaac caagtacaat ccaagctcgc cttactcatc aaccaaggca gcctcagatt
     2041 tgattgtcaa agcctgggtg cgttcttttg gagtcaaggc aacgatttcc aactgttcaa
     2101 ataactacgg tccttaccag catattgaga agtttattcc acgacaaatc accaatatct
     2161 tgagcggtat caagccaaaa ctttacggtg aaggtaagaa cgttcgtgac tggattcata
     2221 ccaatgacca ttcttcagga gtttggacaa tcttgacaaa agggcaaatc ggtgaaacct
     2281 acttgattgg ggctgatggt gagaagaaca ataaggaagt tttggaactt atccttaagg
     2341 aaatgggaca agctgcggat gcctatgatc atgtgactga ccgtgcagga catgaccttc
     2401 gctatgcgat tgatgctagc aagctccgtg atgagttggg gtggaaacct gaatttacca
     2461 actttgaagc tgggctcaag gcaacaatca agtggtatac agataaccaa gaatggtgga
     2521 aagcagaaaa agaagctgtt gaagccaatt atgctaagac tcaggagatt attacagtat
     2581 aaaaagcagg aaatagctgc tttttattgc tatattggga agagttacat attagaaagg
     2641 tctagagatg attttaatta caggggcaaa tggccaatta ggaacggaac ttcgctattt
     2701 attggatgaa cgtaatgaag aatacgtggc agtagatgtg gctaagatgg acattaccaa
     2761 tgaagaaatg gttgagaaag tttttgaaga ggtgaaaccg actttagtct accattgtgc
     2821 agcctacacc gctgttgatg cagcagagga tgaaggaaaa gagttggact tcgccatcaa
     2881 tgtgacgggg acaaaaaatg tcgcaaaagc atctgaaaag catggtgcaa ctctagttta
     2941 tatttctacg gactatgtct ttgatggtaa gaaaccagtt ggacaagagt gggaagttga
     3001 tgaccgacca gatccacaga cagaatatgg acgcactaag cgtatggggg aagagttagt
     3061 tgagaagcat gtgtctaatt tctatattat ccgtactgcc tgggtatttg gaaattatgg
     3121 caaaaacttc gtttttacca tgcaaaatct tgcgaaaact cataagactt taacagttgt
     3181 aaatgaccag tacggtcgtc cgacttggac tcgtaccttg gctgagttca tgacctacct
     3241 agctgaaaat cgtaaggaat ttggttatta tcatttgtca aatgatgcga cagaagatac
     3301 aacatggtat gattttgcag ttgaaatttt gaaagataca gatgtcgaag tcaagccagt
     3361 agattccagt caatttccag ccaaagctaa acgtccgcta aactcaacga tgagcctggc
     3421 caaagccaaa gctactggat ttgttattcc aacttggcaa gatgcattgc aagaatttta
     3481 caaacaagaa gtgagataag tagtagaatg attttctagt ctaataaaag aggcagagaa
     3541 tgaactccaa aggagcttaa gatgtacgat tatcttgttg ttggtgcagt ctttgcccat
     3601 gaagcagtct taaaaggaaa aaaagtaaaa gttattgaaa aatgaaatca tatcgcgggt
     3661 aatatctata ctcgtgaaga ggaaggaatt caagttcatc aatatggtgc tcatatcttc
     3721 catacttctg ataaggagat ctgggattat gtaaatcagt ttgcagactt taaccgttat
     3781 accaattctc ctgttgcaaa ctataagggt gagatttata accttccttt taatatgaat
     3841 accttcaata aactctgggg agttgtgacg ccagcagaag cacaagctaa gattgatgaa
     3901 caacgtgcta ttttaaatgg taaaactcct gaaaatttgg aagaacaggc gatttctctt
     3961 gtaggtacag acatctacga aaaattaatc aaagactata cagagaaaca gtggggcaaa
     4021 ccaactactg aacttccagc ctttattatt gtttgccagt acgcctgacc tatgataaca
     4081 actattttaa cgatacctat caagggattc caattggtgg atacactcaa atagttgaaa
     4141 aatgttggat catgaaaata ttgatgtaga aacaaatgtt gatttctttg tgaataaaga
     4201 gcaatatctg aaagattttc ctaagattgt ctttactggt atgattgatg aattctttga
     4261 ctataagttg ggcgaactag agtaccgtag tcttcgtttt gaaaatgaga ccttggatat
     4321 ggaaaattac caaggaaatg cagttgtgaa ctatacggat gcagaaatcc catatactcg
     4381 cattattgaa cacaaacatt ttgagtttgg gagtcaagca aagactatca ttactaaaga
     4441 acattctaaa acatgggaaa aaggtgatga gccttattat ccagttaata atgatcgtaa
     4501 taatcatttg tataaatcgt ataaaaaact tgctgatgag caagggaatg ttatctttgg
     4561 tggtcgctta ggacactatc gttattacga tatgcaccaa gtaattggag cagctttgca
     4621 gtgcgtgaga aatgagttag attaatactc aatgaaaatc aaagagcaaa ctaggaagct
     4681 agccacaggt tgctcaaaat actgttttga ggttgcagat ggaagctgac gcggtttgaa
     4741 gagattttcg aagagtataa acaagtaaaa ctgactacca gttattattt agaaatagta
     4801 ttaaaaattc cttgactatg tgatatagtt gagggatttt taaatgatat tcatattttt
     4861 tgcaaagatg ttgtttgaaa aataattttc aaaaattctg aaaattctgt tgacatcttt
     4921 ctgaaaagag tttataatgg agagaaagtt ttaaaggaga aaatgatgaa aagttcaaaa
     4981 ctatttgccc ttgcgggcgt gacattattg gcggcgacta ctttagctgc atgctctgga
     5041 tcaggttcaa gcactaaagg tgagaagaca ttctcataca tttatgagac agaccctgat
     5101 aacctcaact atttgacaac tgctaaggct gcgacagcaa atattaccag taacgtggtt
     5161 gatggtttgc tagaaaatga tcgctacggg aactttgtgc cgtctatggc tgaggattgg
     5221 tctgtatcca aggatggatt gacttacact tatactatcc gtaaggatgc aaaatggtat
     5281 acttctgaag gtgaagaata cgcggcagtc aaagctcaag actttgtaac aggactaaaa
     5341 tatgctgctg ataaaaaatc agatgctctt taccttgttc aagaatcaat caaagggttg
     5401 gatgcctatg taaaagggga aatcaaagat ttctcacaag taggaattaa ggctctggat
     5461 gaacagacag ttcagtacac tttgaacaaa ccagaaagct tttggaattc taagacaacc
     5521 atgggtgtgc ttgcgccagt taatgaagag tttttgaatt caaaagggga tgattttgcc
     5581 aaagctacgg atccaagtag tctcttgtat aacggtcctt atttgttgaa atccattgtg
     5641 accaaatctt ctgttgaatt tgcgaaaaat ccgaactact gggataagga caatgtgcat
     5701 attgacaaag ttaaattgtc attctgggat ggtcaagata ccagcaaacc tgcagaaaac
     5761 tttaaagatg gtagccttac agcagctcgt ctctatccaa caagtgcaag tttcgcagag
     5821 cttgagaaga gtatgaagga caatattgtc tatactcaac aagactctat tacgtattta
     5881 gttggtacaa atattgaccg tcagtcctat aaatacacat ctaagaccag cgacgaacaa
     5941 aaggcatcga ctaaaaaggc tctcttaaac aaggatttcc gtcaggctat tgcctttggt
     6001 tttgatcgta cagcctatgc ctctcagttg aatggacaaa ctggagcaag caaaatctta
     6061 cgtaatatct ttgttccacc aacatttgtt caagcagatg gtaaaaactt tggcgatatg
     6121 gtcaaagaga aattggtcac ttatggggat gaatggaagg atgttaatct tgcagattct
     6181 caggatggtc tttacaatcc agaaaaagcc aaggctgaat ttgctaaagc taaatcagcc
     6241 ttacaagcag aaggtgtgac tttcccaatt catttggata tgccagttga ccagacagca
     6301 actacaaaag ttcagcgcgt ccaatctatg aaacaatcct tggaagcaac tttaggagct
     6361 gataatgtca ttattgatat ccaacaacta caaaaagacg aagtaaacaa tattacatat
     6421 tttgctgaaa atgctgctgg cgaagactgg gatttatcag ataatgtcgg ttggggtcca
     6481 gactttgccg atccatcaac ctaccttgat atcatcaaac catctgtagg agaaagtact
     6541 aaaacatatt tagggtttga ctcaggggaa gataatgtag ctgctaaaaa agtaggtcta
     6601 tatgactacg aaaaattggt tactgaggct ggtgatgagg ctacagatgt tgctaaacgc
     6661 tatgataaat acgctgcagc ccaagcttgg ttgacagata gtgctttgat tattccaact
     6721 acatctcgta cagggcgccc aatcttgtct aagatggtac catttacaat accatttgca
     6781 ttgtcaggaa ataaaggtac aagtgaacca gtcttgtata aatacttgga acttcaagac
     6841 aaggcagtca ctgtagatga ataccaaaaa gctcaggaaa aatggatgaa agaaaaagaa
     6901 gagtctaata aaaaggctca agaagatctc gcaaaacatg tgaaataact gttgcaaaat
     6961 ataagaaagg atttagtatt tctcttgaat gctgaatcct tttttacatt tgtaaagaaa
     7021 gattctaaat gtactgcccc ccaaaagttg gagcctcttt ttgtcagaat agagaaaatt
     7081 tttgttaatt ttactt
//
LOCUS       DAVLSL010000096         6901 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLSL010000096 DAVLSL010000000
VERSION     DAVLSL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.098"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            73..1350
                     /locus_tag="VXN30_001526"
     CDS             73..1350
                     /locus_tag="VXN30_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358131.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            1363..2010
                     /gene="vex2"
                     /locus_tag="VXN30_001527"
     CDS             1363..2010
                     /gene="vex2"
                     /locus_tag="VXN30_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1358132.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            2062..3441
                     /gene="vex3"
                     /locus_tag="VXN30_001528"
     CDS             2062..3441
                     /gene="vex3"
                     /locus_tag="VXN30_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1358133.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            3621..3704
                     /gene="pep27"
                     /locus_tag="VXN30_001529"
     CDS             3621..3704
                     /gene="pep27"
                     /locus_tag="VXN30_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1358134.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            3753..4409
                     /gene="vncR"
                     /locus_tag="VXN30_001530"
     CDS             3753..4409
                     /gene="vncR"
                     /locus_tag="VXN30_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1358135.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            4406..5734
                     /gene="vncS"
                     /locus_tag="VXN30_001531"
     CDS             4406..5734
                     /gene="vncS"
                     /locus_tag="VXN30_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1358136.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            5875..6756
                     /locus_tag="VXN30_001532"
     CDS             5875..6756
                     /locus_tag="VXN30_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1358137.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
BASE COUNT         2116 a         1348 c         1516 g         1921 t
ORIGIN      
        1 tttttttttt cgtatttcat aaacatttca tatttgggtt ttataatagt cttacaaata
       61 tggaggtgac aaatgaatcc aatccaaaga tcttgggctt atgtcagcag aaagcgactg
      121 agaagtttta ttttatttct gattttattg gtcttattgg ccggaatttc agcctgtttg
      181 actctgatga agtccaacaa aacagtagaa agcaatcttt ataaatcact caatacatct
      241 ttttctatta agaagataga gaatggtcag acattcaagt tgtcagacct agcatctgta
      301 agcaagatta aggggctgga aaatgtctct cctgaacttg agacggtcgc aaaactaaaa
      361 gacaaggaag cagtgactgg cgagcagagc gtggagcgtg atgatttatc agctgcagac
      421 aataacttgg ttagcttaac ggctcttgag gattcatcca aggatgtaac ctttaccagt
      481 tcggctttca atctaaaaga agggcgacac cttcaaaaag gggattccaa gaaaatcctt
      541 atccacgaag aattggctaa gaagaacggt ctttcacttc atgacaagat tggcttggat
      601 gctggtcagt ctgaatctgg aaaaggacaa acagtagagt ttgagattat tggcatcttt
      661 tctggtaaaa aacaagagaa attcacaggc ttgtcttctg acttcagtga aaatcaagtc
      721 tttacagact atgaaagtag ccaaaccctt ttgggcaata gtgaagctca agtcagtgca
      781 gcacgcttct atgtagaaaa tcctaaggaa atggacggac tcatgaagca ggtagaaaac
      841 ttggccttgg aaaatcaagg ctaccaagtc gaaaaggaaa acaaggcttt tgaacaaatc
      901 aaagactcag ttgcaacttt ccaaaccttc ctgaccatct tcctttatgg gatgttgata
      961 gcaggagctg gagccttaat tctggttttg tctctctggt tgagagaacg ggtctatgaa
     1021 gtggggattt tacttgcact tggaaaaggc aagagctcga tcttcctaca attctgttta
     1081 gaggtagttt tggtatctct tggagctttg cttccagcat ttgttgcagg aaacggaatc
     1141 acaacttacc tactccaaac tctactagca agtggagatc aggcaagctt acaagataca
     1201 ctagccaaag caagcagttt atcaactagc atcttatctt ttgcagaatc ctatgttttt
     1261 ctagttctgc ttagttgctt atctgtagcc ctttgtttcc tattcttatt tagaaaatca
     1321 ccgaaagaaa ttttatcatc tattagttaa gaaggagaaa tcatgacttt attacaatta
     1381 caagatgtta cctaccgtta taagaatact gctgaagcag tcctatatca gatcgattat
     1441 aattttgaac ccggaaaatt ttacagtatt attggggagt caggagcagg aaaatccaca
     1501 ctcttgtccc tacttgctgg tctagatagt cctgttgaag gttctatcct ttttcaagga
     1561 gaggatattc gtaagaaggg ctattcttac catcgcatgc accatatttc cctggtcttt
     1621 caaaattata acttgataga ttatctttct ccgctggaaa atatccgctt ggtcaacaaa
     1681 aaggcaagca aggatacact tcttgagctt ggtttggatg aaagccagat caagcggaat
     1741 gttctccagt tatcaggtgg tcaacagcaa cgtgttgcca ttgctcgcag tttggtctca
     1801 gaagctccag ttattctagc tgatgagcca acaggaaatc tggatcctaa aactgctgga
     1861 gatattgtcg aactactcaa atcacttgcc cagaaaacag gtaaatgtgt gattgtcgta
     1921 actcacagta aagaagtggc acaagcgtca gatattacac ttgaattaaa ggataagaaa
     1981 ctgactgaaa cgcgcaatac tagtaaataa tttgagctta ttttaataga atgattaaaa
     2041 caaaatctag aaagggaatc tatgttacac aacgcatttg cctatgttac aaggaagttt
     2101 ttcaaatcga ttgtcatctt cctgattatt ctcctcatgg cgagcttgag tttggtcggc
     2161 ttgtcaatca agggagctac tgccaaggct tctcaggaga cctttaaaaa tatcaccaat
     2221 agcttctcca tgcaaatcaa tcgtcgcgtc aaccaaggaa cgcctcgtgg tgctgggaat
     2281 atcaagggtg aagacatcaa aaaaatcacc gaaaacaagg ccattgagtc ttatgtcaaa
     2341 cgtatcaacg ctatcggaga tttgactgga tatgacctga ttgaaacgcc agaaaccaag
     2401 aagaatctca ctgctgatcg tgccaagcgt tttggaagta gcttgatgat tacaggtgtc
     2461 aatgactcct ctaaagaaga caagtttgtc tctggttctt ataaactagt cgaaggagag
     2521 cacttaacca acgacgacaa ggataaaatc ctcttgcaca aggacttggc agccaaacac
     2581 ggctggaaag taggggacaa ggttaaactg gactctaata tctacgatgc agataatgaa
     2641 aaaggagcca aggaaacagt tgaagtgaca atcaagggac tctttgatgg tcataataag
     2701 tcagcagtaa cctactcaca agaactttac gaaaacacag ctattacaga cattcacact
     2761 gctgcaaaac tttatggata cacagaagac acagccattt atggggacgc aaccttcttt
     2821 gtaacagcag acaagaactt ggatgatgtt atgaaagagt tgaatggcat cagtggtatc
     2881 aactggaaga gctacacact cgtcaagagc tcctctaact acccagctct tgagcaatct
     2941 atctctggta tgtacaagat ggccaacctc ctcttctggg gtagcttgag cttctcagtt
     3001 ctcctccttg ccctcttgct cagcctttgg atcaacgccc gtcgcaagga agtgggaatt
     3061 ctcctctcta tcggcctcaa gcaggcaagt atcttgggtc aattcatcac cgaatctatc
     3121 ttgattgcta tccctgctct agtttctgct tacttcctag ctaattacac tgcccgtgca
     3181 attggaaaca ctgtccttgc caatgtgact tcaggtgttg ccaaacaggc tagtaaggcg
     3241 gctcaagcct ctaaccttgg tggtggtgca gaagtagatg gctttagcaa gaccttgtcg
     3301 agcctagaca tttccattca gacatcagac tttatcatca tttttgtcct tgccttggtt
     3361 ctagtggttc tcgttatggc gcttgcttca agcaatctcc ttagaaaaca accaaaagag
     3421 ctcttgctgg atggtgaata aatttgaaaa aatgagtcta gaataaagat tgcatcttgt
     3481 gtttctattc aagaatagtg gataggaatg gctatttaac aattcaaaat aaatccgaaa
     3541 gcagtggtga aaatcattgc tttcagttgc tttctttgta ctttagtgct taaatataat
     3601 atactaaagt tatggaattt atgagaaagg aatttcacaa cgttttatct agtggtcagt
     3661 tgcttgcaga caaaaggcca gcaagagact ataatagaaa atagggtagg tatttattct
     3721 aagaaaaata aaaaatagag agcagttaaa gtatgaaaat tttaattgta gaagatgaag
     3781 agatgatccg tgagggggtc agtgattatt tgacggattg tggctatgaa actattgagg
     3841 cagcggacgg tcaggaagct ctggagcaat tttctagcta tgaggtggcc ctggttttac
     3901 tggatatcca gatgcccaag ctcaacggct tagaagtcct agctgagatt cgtaaaacca
     3961 gtcaggttcc tgtcttgatg ttgacagctt ttcaagatga ggaatacaag atgagtgcct
     4021 ttgcctcttt ggcagatggc tatctggaaa aacctttctc cctctccctt ttaaaagtga
     4081 gggtggacgc gattttcaag cgctactacg atacaggacg aatcttttct tacaaggata
     4141 ccaaggtgga ctttgaaagc tacagtgcaa gcctcgcagg tcaagaagtg cctatcaatg
     4201 ccaaagagtt ggaaattctg gactatctag tgaaaaatga aggccgggcc ttgactcgat
     4261 ctcagattat cgatgccgtc tggaaagcga cagatgaggt tccctttgac cgtgttattg
     4321 atgtttatat caaggaattg cggaaaaagc tagatttgga ttgtatcctc actgtgcgca
     4381 atgttggtta taaattggag cgaaaatgaa acgaacaggt ttatttgcaa agatatttat
     4441 ctataccttc tcgatattta gtgttctggt tatctgcctt catttagcta tttattttct
     4501 ttttccttcg acttatctga gtcatcgtca ggaaaccatt ggtcaaaagg caacagccat
     4561 tgcccagtcc ctagaaggga aagataggca gagtatcgag caagtgttag acttgtattc
     4621 ccagactagt gatatcaagg ggaccgtcaa aggtgagatg accgaggaca agttagaagt
     4681 caaggacagt cttcctctgg acacagaccg ccagacaacc tctctcttta ttgaggagcg
     4741 cgaggtgaaa acgcaagacg gtggtactat gattctccag tttctagctt ccatggattt
     4801 acaaaaggaa gcggagcaaa tcagtctcca gtttcttccc tataccttgc tggcctcctt
     4861 tctgatttcc cttttggtgg cctacatcta cgctcggact attgttgcac cgattttgga
     4921 aatcaagcgg gtgacccgtc ggatgatgga cctggattcc caagtgcgat tgcgcgtgga
     4981 ttctaaggat gagataggta atctcaagga acaaatcaat agcctctacc agcatctctt
     5041 gactgttatt gcggacttgc atgaaaagaa tgaagccatt ctccagctgg agaagatgaa
     5101 ggtcgaattc ctacgaggag cttctcatga attgaaaaca ccgctggcta gtttgaaaat
     5161 cctaatcgaa aatatgagag agaatatcgg tcgttataag gatagagacc agtatctggg
     5221 agttgccttg gggattgtgg atgaactcaa tcaccatgtt ctgcagatac tttccctctc
     5281 ttctgtgcag gaattgcgag atgataggga aacaattgac ctcctccaga tgacgcaaaa
     5341 tctggtcaaa gattatgcct tgctagccaa ggaaagagag ctccagatag acaatagttt
     5401 gacccatcag caggcttatc taaacccatc agttatgaag ttgattcttt ctaatctcat
     5461 cagcaatgcc attaagcact ctgttccagg tggcttagtt cgaattggag aaagagaagg
     5521 agaacttttt atcgaaaata gctgtagctc agaggaacaa gaaaaactag cccagtcttt
     5581 ttctgacaat gccagtcgca aggtcaaggg gtctggtatg gggctctttg tggttaagag
     5641 tctattagaa catgaaaaat tagcttatcg tttcgagatg gaggagaata gtttaacctt
     5701 ctttatagat tttccaaaag tcgtccaaga ctagggagag aaagggttta catagatgga
     5761 gttagaagaa aatcaatcga aactgcggga aaaactagat ttttttggca aaaagtgata
     5821 aaatgaacaa tgtaaatggg atgacccata aaaatataca ggaggcctga taaaatggca
     5881 atcgtttcag cagaaaaatt tgtccaagca gcccgtgaca acggttatgc agttggtgga
     5941 tttaacacaa acaaccttga gtggactcaa gctatcttgc gcgcagcaga agctaaaaaa
     6001 gctccagttt tgatccaaac ttcaatgggt gctgctaaat acatgggtgg ttacaaagtt
     6061 gctcgcaact tgatcgctaa ccttgttgaa tcaatgggta tcactgtacc agtagctatc
     6121 caccttgacc acggtcacta cgaagatgca cttgagtgta tcgaagttgg ttatacttca
     6181 atcatgtttg acggttcaca ccttccagtt gaagaaaacc ttaaattggc taaagaagtt
     6241 gttgaaaaag cacacgctaa aggtatctca gtagaagctg aagttggtac tatcggtggt
     6301 gaagaagacg gaatcatcgg taaaggtgaa ttggctccaa tcgaagacgc taaagcaatg
     6361 gttgaaactg gtatcgactt cttggcagct ggtatcggta acatccacgg cccttaccca
     6421 gtaaactggg aaggtcttga ccttgaccac ttgcaaaaat tgacagaagc tcttccagga
     6481 ttcccaatcg tattgcacgg tggatcaggt attcctgatg agcaaatcca agcagctatc
     6541 aaacttggtg ttgccaaagt taacgttaac acagaatgcc aaatcgcatt cgctaacgca
     6601 actcgtaaat ttgctcgtga ctacgaagca aacgaagcag aatacgacaa gaaaaaactc
     6661 ttcgacccac gtaaattctt ggctgacggt gtaaaagcta tccaagcatc ggttgaagaa
     6721 cgtatcgacg tattcggttc agaaggtaaa gcataatcta gctgaataat acagataaaa
     6781 cctgcctgtg agggtgggtt ttttgatttc aagctactcg ttttagtata gtaaactgaa
     6841 tctagactag cacaatgctg cttctatagc gttttgaaac tagaatagga caacataact
     6901 g
//
LOCUS       DAVLSL010000097         6894 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLSL010000097 DAVLSL010000000
VERSION     DAVLSL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.099"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            87..3437
                     /locus_tag="VXN30_001533"
     CDS             87..3437
                     /locus_tag="VXN30_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1358138.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3708..3908
                     /locus_tag="VXN30_001534"
     CDS             3708..3908
                     /locus_tag="VXN30_001534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358139.1"
                     /translation="MKIKEQTRKLAASCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(4025..4495)
                     /locus_tag="VXN30_001535"
     CDS             complement(4025..4495)
                     /locus_tag="VXN30_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1358140.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4512..6785)
                     /locus_tag="VXN30_001536"
     CDS             complement(4512..6785)
                     /locus_tag="VXN30_001536"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1358141.1"
                     /translation="MRFNQYSYINFPKENILSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSENELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSSQDVIREVVYVESRVDTDQKGLPDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVSLPHKIELEKPQLNLVQPQGKAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFATLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNDRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2113 a         1163 c         1496 g         2122 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtagaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatggcttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cacttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggctgggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgc atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt tcactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttgttttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctctgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctttggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaagctgct caaaacactg ttttgaggtt gtagataaga
     3781 ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta
     3841 accataccta cggcaaggcg acgttgacgc ggattgaaga gattttcgaa gagtataaat
     3901 cttcctagga taaagcaaaa cgcatagtat caagggtttt cagcacttga tactatgcgt
     3961 tttctgatgt taaagacttt ctaccaggtt ttttaaaagc ataattgtta gttgtagtca
     4021 tttattattc ttcaaagaaa aatggtgggg cgaatttttt cagttcttca aagcactttt
     4081 gagcagtatc tgcatcttca cagatgataa gacagacatc attaccacaa agggtagcga
     4141 tagcgtcagg gaagctcaaa gtatcaatga tagaaccaaa ggattgagcc agtccaggaa
     4201 gggtttttag taggacttgg tgttgaactg ggcgcatcca gacaagggcg tcttccatgt
     4261 agagttcgag acgtttttcc cattttgaga tggaaccatt gttaagaaca taataagcgc
     4321 tatcttcttc gcggactttt gataggttca tatttttgat gtcgcgtgag agggttgcct
     4381 gggttacttg aatgtcgttc tcagcaagaa gggcttgcaa ctcagcctgt gtatgaatct
     4441 tgttttttgt gataagagcg cgtataagtt ggtggcggtg ttctgattta ttcataataa
     4501 tgtaactcct tttagcaagg taaggtaagc atggactgag cgaggtcgac agtcaagtgg
     4561 tagtctgtat tgtcacggat ggtgatttca aagtcagtag tatagaggac taaacggaga
     4621 gtgtctcctt cttttagctt gtaaatagtt ggctgcagtt caaattgaac gtccatccat
     4681 tcatctgcag taatatcctc tactaacagt aaatcatttc tattttgtaa attaaggtaa
     4741 ccttttgtca cgactcgttg tgcctctggt ctaaatggca attcacagag attttccaac
     4801 atgtgatagc gaccgttatc aatggttcta gcacttaaaa tagctggata aggttgtagg
     4861 tatttctttt gcccaaattc tagcagttgg gcagataaga gccccttgtt tgtactggat
     4921 ttgatacgaa gattgagctg agcgcgaccg tttaggtgga gatctttagt cacaggaagg
     4981 ttaatagtaa tctgattggc tttcccttga tagagctctg tattgaaggt ttggtatgtc
     5041 ttaccatagc gctcaaaatc cttatctggg tactggtttt gaatagcttg ctcttcttga
     5101 ccaagtgaga aggtttcaca gttttcttgc ccaccgaagt tatcaagtga taaccaagtc
     5161 tgtggagctg tattgtcctg ccagataaca gtaggaagtt gaaagtctgt ttcctgtcct
     5221 agtaatttct tggtcaataa ggcatttatg gactcacgga agtcaattga ttgccaattg
     5281 ttcatgtaaa catgagcacc attatggaaa aagagatgct tgtgtatatg agtaggaaga
     5341 gcatggaaca tctggtaaac atgaagtggt ttgacattcc aatcctgaga accatgagta
     5401 aagacaacct ctgcctttac tttatgggca ttgagcagat aattgcggtc atgccaaaac
     5461 tgattgtagt ccccagtttt tcggtctagc tgagctttca ctttttctaa gtcagcttgg
     5521 tgagcttcat tgccacggat atagtcgcca gctaagagat tacgagaata ggttaactca
     5581 gcaagggagt caaagtcctc acctggataa ccacctgggc tagtcaccag accgttttca
     5641 cggtagtagt tgtaccatga tgaaattcct gcctcggcaa tgataacttc taaaccatcg
     5701 actcctgtag tcgcaagacc attggacatg gtacctagat aggaaagtcc cgttgtagca
     5761 acttttccgt ttgaccaatc agccttgact tgacgctggc gcgtgtgatc agtaaaggca
     5821 cggcaacggt cgttaagcca atcgatgaca tttttataag cctcgatttg ctggtagtct
     5881 ccattagtca tgaaacctgt cgagtctttg gtaccaacac ctgagacata gagagtggca
     5941 aagcctctcg gaaggaagta gtcgtttagt gtatagctag agttgatgtg agttagcttt
     6001 tcctcagcct ctgctacaag ctcagcttta ccttggggtt ggacgagatt tagttgaggt
     6061 ttctctagct caatcttgtg aggaagagaa acctcaagct cgccctccat cttgtagaga
     6121 gccttgtcac tagccttatc attggttccc tgatgataag ggctggctgt catgatggca
     6181 gggatttttc catcaaaacg agggcgaata atgctaacct ttactaggtc tggtagccct
     6241 ttttggtcag tatcgacacg agactcaacg taaacgactt cacgaatgac atcctggcta
     6301 gaaaaagtag ccaaactctt gccgttaaag tagtggtagt cattatcctc cggaataaga
     6361 ccatcactaa caagttggtc gataagagta tttccttttt tggtgcgagt attgagtaac
     6421 tgatagagat tttcaatcaa gtcaccatat ataatgggaa atccagtttc tttacgaaaa
     6481 acgtcactat cttcgaagtc aaccaaataa gaaaagccta aaagttgaaa agcaacagta
     6541 taaaaaatat ctgctgtcag ttcattttcc gattgaaaaa atgtcagcag gtctgttttt
     6601 ttatcagctg ctaggatgga aagtgggtag ttggtgtctt gataagtgaa aaagaaacga
     6661 cgtaaaaagg tttcaagtga gtctttgtga ttggctgtat tttgtaaatc aaagccacat
     6721 ttttttagtt cagataagat attttctttt ggaaaattga tataactata ttgattaaaa
     6781 cgcatagaac ctccatatag aatgacagtt aaggttatta tatcaaaaaa aaagcagaaa
     6841 gggaattgtt aacttcaaaa ggaaataatc caataaaaat gaataaagta ctaa
//
LOCUS       DAVLSL010000098         6889 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLSL010000098 DAVLSL010000000
VERSION     DAVLSL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.100"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            2..6436
                     /locus_tag="VXN30_001537"
     CDS             2..6436
                     /locus_tag="VXN30_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1358142.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMTFDLYANINDIVDGLAFAGDMRFFVKDDNRIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         2698 a          951 c         1344 g         1896 t
ORIGIN      
        1 aatgaaaaaa agtacagtat tgtcattaac cacagctgca gttattttag cagcctatgc
       61 ccctaatgag gtagtcttag cagacacatc tagctctgaa gatgctttaa acatctctga
      121 taaagaaaaa gtagtattag ataaggaaac agaaaataaa gagaaacatg aagatattca
      181 taatgctata gaaacttcaa aggatactga agaaaagaaa acaacagtta ttgaggaaaa
      241 agaagttgtt agtaaaaatc ctgtgataga cactaaaact agcaatgaag aagcaagaat
      301 caaagaagat tccaatcaat cccaaggaga taatgcacac tcgtctgcaa ataaaggcac
      361 agaaaatccg aaaaaagaag atagacttgt ctatattgct gaatttaaag ataaagaatc
      421 tggagaaaaa gcaatcaagc aactatccaa tcttaagaat acaaaagttt tatatactta
      481 tgatagaatt tttaacggta gtgccataga aacaactcca gataacttgg acaaaattaa
      541 acaaatagaa ggtattacat cggttgaaag ggcacaaaaa gtccaaccca tgatgaatca
      601 tgccagaaag gaaattggag ttgaggaagc tattgattac ctaaagtcta tcaatgctcc
      661 gtttgggaaa aattttgatg gtagaggtat ggtcatttca aatatcgata ctggaacaga
      721 ttatagacat aaggctatga gaatcgatga tgatgccaaa gcctcaatga gatttaaaaa
      781 agaagactta aaaggaactg ataaaaatta ttggttgagt gataaaatcc ctcatgcgtt
      841 caattattat aatggtggca aaatcactgt agaaaaatat gatgatggaa gggattattt
      901 tgacccacat gggatgcata ttgcagggat tcttgctgga aatgatactg aacaagatat
      961 caaaaacttt aacggcatag atggaattgc acctaatgca caaattttct cttacaaaat
     1021 gtattctgac gcaggatctg ggtttgcggg tgatgaaaca atgtttcatg ctattgaaga
     1081 ttctatcaaa cacaacgttg atgttgtttc ggtatcatct ggttttacag gaacaggtct
     1141 tgtaggtgag aaatattggc aagctattcg ggcattaaga aaagcaggca ttccaatggt
     1201 tgtcgctacg ggtaactatg cgacttctgc ttcaagttct tcatgggatt tagtagcaaa
     1261 taatcatctg aaaatgaccg acactggaaa tgtaacacga actgcagcac atgaggatgc
     1321 gatagcggtc gcttctgcta aaaatcaaac agttgagttt gataaagtta acataggtgg
     1381 agaaagtttt aaatacagaa atataggggc ctttttcgat aagaataaaa tcacaacaaa
     1441 tgaagatgga acaaaagctc ctagtaaatt aaaatttgta tatataggca aggggcaaga
     1501 ccaagatttg ataggtttgg atcttagggg caaaattgca gtaatggata gaatttatac
     1561 aaaggattta aaaaatgctt ttaaaaaagc tatggataag ggtgcacgcg ccattatggt
     1621 tgtaaatact gtaaattact acaatagaga taattggaca gagcttccag ctatgggata
     1681 tgaagcggat gaaggtacta aaagtcaagt gttttcaatt tcaggagatg atggtgtaaa
     1741 gctatggaac atgattaatc ctaataaaaa aactgaagtc aaaagaaata ataaagaaga
     1801 ttttaaagat aaattggagc aatactatcc aattgatatg gaaagtttta attccaacaa
     1861 accgaatgta ggtgacgaaa aagagattga ctttaagttt gcacctgaca cagacaaaga
     1921 actctataaa gaagatatca tcgttccagc aggatctaca tcttgggggc caagaataga
     1981 tttactttta aaacccgatg tttcagcacc tggtaaaaat attaaatcca cgcttaatgt
     2041 tattaatggc aaatcaactt atggctatat gtcaggaact agtatggcga ctccaatcgt
     2101 ggcagcttct actgttttga ttagaccgaa attaaaggaa atgcttgaaa gacctgtatt
     2161 gaaaaatctt aagggagatg acaaaataga tcttacaagt cttacaaaaa ttgccctaca
     2221 aaatactgcg cgacctatga tggatgcaac ttcttggaaa gaaaaaagtc aatactttgc
     2281 atcacctaga caacagggag caggcctaat taatgtggcc aatgctttga gaaatgaagt
     2341 tgtagcaact ttcaaaaaca cggattctaa aggtttggta aactcatatg gttccatttc
     2401 tcttaaagaa ataaaaggtg ataaaaaata ctttacaatc aagcttcaca atacatcaaa
     2461 cagacctttg acttttaaag tttcagcatc agcgataact acagattctc taactgacag
     2521 attaaaactt gatgaaacat ataaagatga aaaatctcca gatggtaagc aaattgttcc
     2581 agaaattcac ccagaaaaag tcaaaggagc aaatatcaca tttgagcatg atactttcac
     2641 tataggcgca aattctagct ttgatttgaa tgcggttata aatgttggag aggccaaaaa
     2701 caaaaataaa tttgtagaat catttattca ttttgagtca gtggaagaaa tggaagctct
     2761 aaactccaac gggaagaaaa taaacttcca accttctttg tcgatgcctc taatgggatt
     2821 tgctgggaat tggaaccacg aaccaatcct tgataaatgg gcttgggaag aagggtcaag
     2881 atcaaaaaca ctgggaggtt atgatgatga tggtaaaccg aaaattccag gaaccttaaa
     2941 taagggaatt ggtggagaac atggtataga taaatttaat ccagcaggag ttatacaaaa
     3001 tagaaaagat aaaaatacaa catccctgga tcaaaatcca gaattatttg ctttcaataa
     3061 ccaagggatc aacgctccat catcaagtgg ttctaagatt gctaacattt atcctttaga
     3121 ttcaaatgga aatcctcaag atgctcaact tgaaagagga ttaacacctt ctccacttgt
     3181 attaagaagt gcagaagaag gattgatttc aatagtaaat acaaataaag agggagaaaa
     3241 tcaaagagac ttaaaagtca tttcgagaga acactttatt agaggaattt taaattctaa
     3301 aagcaatgat gcaaagggaa tcaaatcatc taaactaaaa gtttggggtg acttgaagtg
     3361 ggatggactc atctataatc ctagaggtag agaagaaaat gcaccagaaa gtaaggataa
     3421 tcaagatcct gctactaaga taagaggtca atttgaaccg attgcagaag gtcaatattt
     3481 ctataaattt aaatatagat taactaaaga ttacccatgg caggtttcct atattcctgt
     3541 aaaaattgat aacaccgccc ctaagattgt ttcggttgat ttttcaaatc ctgaaaaaat
     3601 taagttgatt acaaaggata cttatcataa ggtaaaagat cagtataaga atgaaacgct
     3661 attcgcgaga gatcaaaaag aacatcctga aaaatttgac gagattgcga acgaagtttg
     3721 gtatgctggc gccgctcttg ttaatgaaga tggagaggtt gaaaaaaatc ttgaagtaac
     3781 ttacgcaggt gagggtcaag gaagaaatag aaaacttgat aaagacggaa ataccattta
     3841 tgaaattaaa ggtgcgggag atttaagggg aaaaatcatt gaagtcattg cattagatgg
     3901 ttctagcaat ttcacaaaga ttcatagaat taaatttgct aatcaggctg atgaaaaggg
     3961 gatgatttcc tattatctag tagatcctga tcaagattca tctaaatatc aaaagcttgg
     4021 cgagattgca gaatctaaat ttaaaaattt aggaaatgga aaagagggta gtctaaaaaa
     4081 agatacaact ggggtagaac atcatcatca agaaaatgaa gagtctatta aagaaaaatc
     4141 tagttttact attgatagaa atatttcaac aattagagac tttgaaaata aagacttaaa
     4201 gaaactcatt aaaaagaaat ttagagaagt tgatgatttt acaagtgaaa ctggtaagag
     4261 aatagaggaa tacgattata aatacgatga taagggaaat attattgctt atgacgatgg
     4321 tagtgcctta caatatgaaa ctgaaaaact tgacgaaata aaatcaaaaa tttatggcgt
     4381 tctaagccca tctaaagatg gacactttga aattcttgga aagataagca atgtttctaa
     4441 aaatgccaag gtatattatg gaaatagcta taaatcgata gaaatcaaag cgactaagta
     4501 tgactcacat tcaaaaacga tgacatttga tttatacgct aatattaatg atattgtgga
     4561 tggattagct tttgctggag atatgcggtt ttttgttaaa gatgataatc gaataaaagc
     4621 tgaaactaaa attagaatgc ctgaaaaaaa taaggaaact aaagcagaat atccctatgt
     4681 atcaagttat gggaatgtaa tcgaattagg agaaggagat ctttcaaaga acaaaccaga
     4741 caatttaact aaaatggaat ctggtaaaat ctattctgat tcagaaaaac aacaatatct
     4801 gttaaaggat aacatcattc taagaaaagg ctatgcacta aaagtgacta cctataatcc
     4861 tggaaaaacg gatatgttag aaggaaatgg agtctatagc aaggaagata tagcaaaaat
     4921 acaaaaggcc aatcctaatc taagagtcct ttcagaaaca acaatttatg ctgatagtag
     4981 aaatgttgaa gatggaagaa gtacccaagc tgtattaatg tcggctttgg acggctttaa
     5041 cattataagg tatcaagtgt ttacatttaa aatgaacgat aaaggggaag ctatcgataa
     5101 agacggaaat cttgtgacag attcttctaa acttgtatta tttggtaagg atgataaaga
     5161 atacactgga gaggataagt ccaatgtaga agctataaaa gaagatggct ccatgttatt
     5221 tattgatacc aaaccagtaa acctttcaat ggataagaac tactttaatc catctaaatc
     5281 taataaaatt tatgtacgaa atccagaatt ttatttaaga ggtaagattt ctgataaggg
     5341 tggttttaac tgggaattga gagttaatga atcggttgta gataattatt taatctacgg
     5401 agatttacac attgataaca ctagagattt taatattaag ctgaatgtta aagacggtga
     5461 catcatggac tggggaatga aagactataa agcaaacgga tttccagata aggtaacaga
     5521 tatggatgga aatgtttatc ttcaaactgg ctatagcgat ttgaatgcta aagcagttgg
     5581 agtccactat cagtttttat atgataatgt taaacccgaa gtaaacattg atcctaaggg
     5641 aaatactagt atcgaatatg ctgatggaaa atctgtagtc tttaacatca atgataaaag
     5701 aaataatgga ttcgatggtg agattcaaga acaacatatt tatgtaaatg gaaaagaata
     5761 tacatcattt gatgatatta aacaaataac agacaagaca ctaaacatta agattgttgt
     5821 aaaagatttt gcaagaaata caaccgtaaa agaattcatt ttaaataaag atacgggaga
     5881 ggtaagtgaa ttaaaacctc atagggtaac tgtgaccatt caaaatggaa aagaaatgag
     5941 ttcaacgata gtgtcggaag aagattttat tttacctgtt tataagggtg aattagaaaa
     6001 aggataccaa tttgatggtt gggaaatttc tggtttcgaa ggtaaaaaag acgctggcta
     6061 tgttattaat ctatcaaaag atacctttat aaaacctgta ttcaagaaaa tagaggagaa
     6121 aaaggaggaa gaaaataaac ctacttttga tgtatcgaaa aagaaagata acccacaagt
     6181 aaaccatagt caattaaatg aaagtcacag aaaagaggat ttacaaagag aagatcattc
     6241 acaaaaatct gattcaacta aggatgttac agctacagtt cttgataaaa acaatatcag
     6301 tagtaaatca actactaaca atcctaataa gttgccaaaa actggaacag caagcggagc
     6361 ccagacacta ttagctgccg gaataatgtt tatagtagga atttttcttg gattgaagaa
     6421 aaaaaatcaa gattaagaca aaagctatag aaaaaaatgg tttatgtact gagattagat
     6481 agtgaggtga tgacatagtt ttgtgaaaac ggccatttat aactcaatta tttagtttac
     6541 ttcacttcac tattgatact atttggagtt attaatggac ttagtttaga taactaatga
     6601 attgattgaa agggttagta ttgacaatat tggtcatatt gattagaaaa tagagtctat
     6661 caaaatttaa aggctaatag aggtgatgag acaatttcaa ggaattaagt agcgtttggt
     6721 tgcttaattc ctttttattt ggaaggaagt ttttcgtaac cgcccaataa cgaagtctat
     6781 tggacggtta cgatatgatc actgacttcg tcagtcttat ctacaacctc aaaactgtgt
     6841 tttgagcaac ctgcggctag cttcctagtt tactctttga ttttcattg
//
LOCUS       DAVLSL010000099         6596 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLSL010000099 DAVLSL010000000
VERSION     DAVLSL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.101"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            13..876
                     /locus_tag="VXN30_001538"
     CDS             13..876
                     /locus_tag="VXN30_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358143.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIQAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELGTV"
     gene            1259..1879
                     /locus_tag="VXN30_001539"
     CDS             1259..1879
                     /locus_tag="VXN30_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358144.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYTGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1965..2408
                     /gene="tsaE"
                     /locus_tag="VXN30_001540"
     CDS             1965..2408
                     /gene="tsaE"
                     /locus_tag="VXN30_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1358145.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2398..2916
                     /locus_tag="VXN30_001541"
     CDS             2398..2916
                     /locus_tag="VXN30_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358146.1"
                     /translation="MEYELLIREAEPKDAAELVTFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2924..3940
                     /locus_tag="VXN30_001542"
     CDS             2924..3940
                     /locus_tag="VXN30_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1358147.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4025..5281
                     /locus_tag="VXN30_001543"
     CDS             4025..5281
                     /locus_tag="VXN30_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1358148.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5336..6502
                     /gene="recA"
                     /locus_tag="VXN30_001544"
     CDS             5336..6502
                     /gene="recA"
                     /locus_tag="VXN30_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1358149.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         2094 a         1080 c         1489 g         1933 t
ORIGIN      
        1 ttattatctt taatgagtac actcgcagag aaattcagat tgaaaagaaa agagttgaga
       61 ctctcccaac aaactcttgc agaaggaatt tgtgaacaaa gccagattag taaaattgag
      121 agagggcatt tcattccctc cgcagacctt ttgttcaaac tctctcaacg acttgaagta
      181 ccattagatt atttttttaa tgaacaaatt gaaattaaat ctaacctctc taatttcaag
      241 caattgtctg ctcgactatt agacgataga aattatgacg atttggaata tatttataga
      301 atagagattg aacgaagtac ttttctaaca ctagaagacc gaacttacct tgaatggatt
      361 caagctatta ttgacttcta tcaatatgac agtaagtgtg aggcaatttc ttcattggaa
      421 aatatattat taaaagtctc ttcaaatact ctgatttatt taaaggcgtt gaatactcta
      481 tctaatttct attccttagt gggtcgtgaa caagaatatg aggcaaacta ctctcattta
      541 atagagttgt atcagacaaa aaatttggac catcaagagt ttttatttgg ctacatcaga
      601 gttcgttaca actacgctca ctacctagta tcaaaggaaa aatataatga agccatccaa
      661 gaagctcttg aaacgattga actctgtaaa caaagacaga caagctacca attggctcct
      721 ctacttattc ttgtaggaaa tgctggagcc aaatttctag acagagaaca agtcaaaaat
      781 tattatatag aagcaagaga gttatgtaag atttataaca atcctttaat gttgatgaag
      841 atagaaaatt atttgaagga actaggtact gtttagttaa tcttgacatt ataatttttc
      901 tgaaacgtta aataacctgt aaggctgata gttagattaa tactatcagc cttataatta
      961 tgtgactctg agtccgtctc gctccgtgag gtgcgagaca aaacaaccac ccgtttgacg
     1021 gattttcatg atttcaaatt tatattcttc gaaaatctct tcaaatcacg tcagctctat
     1081 ctgcaatctc aaaacactgt tttgagcaac ctgtggctag ctacctagtt tgatctttga
     1141 ttttcattga gtattagtct ctagtgtttg tttagcgtct taaagagaca tttgagaaga
     1201 ttagaaacag ttctctcttt tctcctagta taatgattgg taggaaggga gagagaagat
     1261 gaaatcaatg agaatcttat ttttgttagc tttaattcaa atcagtttga gtagctgttt
     1321 cctatggaag gaatgcatct tgtcctttaa acaaagtaca gcttttttca tcggaagcat
     1381 ggttttcgtt tcaggaatct gtgctggagt aaattatctt tatacccgta agcaagaagt
     1441 ccatagtgtc ctagccagta agaagtcggt gaagcttttt tacagtatgt tactcttaat
     1501 taatttgtta ggagctgttc ttgttttgtc agataacttg ttcatcaaaa atacgctgca
     1561 gcaagaatta gttgactttt tattgccatc cttctttttc ctatttgggc tagatttgct
     1621 gattttttta cccttgaaaa aatacgtgcg cgattttctt gctatgctag acagaaaaaa
     1681 gacagtgttg gtgactattt tagcaacact tcttttctta agaaatccaa tgaccattgt
     1741 ctcacttctg atttatactg gactgggctt gttttttgca gcctatcttg tcccaaattc
     1801 ggttaagaag gaagtttcct tttatggtca tattttccga gatcttgtat tggtcattgt
     1861 tacgctcatt ttcttttaga gaaagcctgt tttttatgga tgttatgatg ataagtttag
     1921 taggaaaata gacacaaaag aaaagttttt ggtataataa gagtatgtac acaaaaaatg
     1981 aagaagagtt gcaagcctta ggggagcgtt tgggccatct attagcaaag aatgatgttt
     2041 taatcttaac tggagaactg ggtgcaggta aaacgacctt tactaaagga cttgcaaaag
     2101 gattacagat ttctcaaatg attaaaagtc ccacctatac tatcgtgaga gagtatgaag
     2161 gtcgacttcc actttatcac ctagatgttt atcgtattga aggagatgct gattctatcg
     2221 acttggatga gtttatcttt ggtggcggcg tgactgttat tgagtgggga aatctcttag
     2281 gagatgcctt gccagatgct tatttagaat tggaaattct aaaagaagca gatggacgcc
     2341 gtttaaattt tcaggcaaag ggtttgcgtg ctgagaaatt gttagaggag cttcaatatg
     2401 gagtatgaat tgctcattag ggaagcagag cccaaagatg cagctgaatt agtgaccttt
     2461 ttaaatcgtg tgagtttgga gacagacttt accagcctag acggagatgg tattctcttg
     2521 actagtgagg agatggaaat attcctcaac aagcaagcta gttcggacaa tcagataacc
     2581 ttacttgcat tcttaaatgg caaaattgct ggtattgtaa atattacagc tgatcagcgt
     2641 aagagagtcc gtcatattgg agatctcttc attgtgattg gaaaaagata ttggaataat
     2701 ggcttgggaa gtttgttgct agaagaagcg atagagtggg cacaagcaag tggcattctg
     2761 cgtcgtctcc aactgactgt ccaaactcgt aatcaagcag cagtccatct ttatcaaaag
     2821 catggctttg tcattgaagg tagccaagag cgtggggcat atatagaaga agggaaattt
     2881 atcgatgttt acttgatggg taaactgata gattagtaga aatatggtta aaaaaattat
     2941 tggaatggtg ctagctttac tttctgtaac tgtagtagga gtaggtgttt ttgcttatac
     3001 tatttatcaa caagggacag aaaccttagc taaaacctat aaaaaaatcg gcgaagaaac
     3061 caaggttatt gaagcgactg aacctctaac cattctgtta atgggagtgg acaccggaaa
     3121 tgctgaacga actgaaactt gggtcggtag aagtgatagc atgatcttga tgacagtgaa
     3181 tcctaaaacg aaaaaaacaa caatgatgag tttagagcgg gatattctga cgcgcattga
     3241 atcagggaat ggtcaggctc atgaagcgaa actgaactca gcatatgcag atggtggagc
     3301 agagcttgct atagaaacca ttcaaaaaat gatgaatatc catattgatc gctatgtgat
     3361 ggtcaatatg agagggttgc aaaaactagt ggatgcagta ggaggtatta cagtcaataa
     3421 tatcctaggt ttcccaattt ctatcagtga ccaagaagaa tttaatacta tttctatcgg
     3481 tgttggggag caacatattg ggggagaaga agccctagtc tatgcacgaa tgcgttacca
     3541 agatcctgag ggggattatg gtcgtcaaaa acgtcaacgt gaagttattc aaaaagtcat
     3601 ggaaaaagct ctcagtttaa atagcgttgg tcattatcaa gagattctaa aagctttgag
     3661 tgacaatatg cagaccaata ttgatttgtc tgcaaaaagt atccctaact tgctaggcta
     3721 taaagattca tttaaaacca ttgaaactca gcagttgcag ggtgaaggag agatacttca
     3781 aggtgtttct taccagattg tttcgagagc acatatgttg gaaatgcaaa atatactccg
     3841 acgttctttg ggacaagaag aagttactca gcttgaaacc aatgcggttt tatttgaaga
     3901 tttatttggc agagcacctg ttggtgatga agataattaa cttttgatac aaataaaaaa
     3961 atcaatcgta ggaaaattcc tgcgattttt tcaaaaaaat acgaatagat aggtaggagg
     4021 aaacatgaaa gcagaaatca ttgctgttgg aacagagatt ttgacaggac agattgtcaa
     4081 caccaatgcc cagtttttgt cggaaaaact agctgagatt ggggtagatg tatattttca
     4141 gacggctgta ggagacaatg aagttcgtct cttgtctttg cttgagattg ccagtcaacg
     4201 tagcagtctg gtgattttga caggcggttt ggggccaact gaggacgacc taaccaaaca
     4261 aaccctagct aaatttttag ggaaagcatt agtctttgat cctcaggctc aggagaagtt
     4321 ggatatcttt tttgccctgc gaccagacta tgcccgaaca ccaaataacg aaagacaagc
     4381 tcaaattgta gaaggagcga ttccactgcc aaacgaaaca ggactggctg ttggaggaaa
     4441 attagaagta gacggagtga cctatgtcgt ccttccaggt ccgccaagtg aattgaaacc
     4501 catggtctta aaccaacttc tacccaagtt gatgacaggg agcaagctgt attcccgagt
     4561 tcttcgtttc tttgggattg gcgagagcca gttggttacg attttggctg atttaattga
     4621 taatcagatc gatcctacct tggcccctta tgccaagaca ggagaagtca ctctacgtct
     4681 gtcaacaaag gctagcagtc aagaagaggc gaatcaagcg ctggatatct tggaaaatca
     4741 aatcttggac tgccagactt tcgaaggaat ttctttacga gacttttgct atggttatgg
     4801 ggaagaaact agtttagcca gcattgtggt agaagaactg aaaaggcaag ggaaaaccat
     4861 cgcggctgca gagagtttga cggcaggtct tttccaagct accgtggcga atttttctgg
     4921 agtttcaagt atatttgagg gtggttttgt gacctatagc ttggaggaaa aatcaaggat
     4981 gttggatatt cctgccaaga atttggaaga acatggtgtg gtgtctgaat ttacagctca
     5041 gaagatggct gagcaggcac gaagcaagac ccagtctgat tttggaatta gtttgactgg
     5101 agtggcagga ccagatagcc tagaaggaca cccagttggg acagtcttca taggcttggc
     5161 gcaagatcaa ggaactgagg ttatcaaggt taatattgga ggcagaagcc gagcagatgt
     5221 acgtcacatt gcggttatgc atgcctttaa cctagttcgc aaggctttat taagtgacta
     5281 acttttgata taatagtaga taggtctgag gatcattaga atgtaggaga atagaatggc
     5341 gaaaaaacca aaaaaattag aagaaatttc aaaaaaattt ggggcagaac gtgaaaaggc
     5401 cttgaatgac gctcttaaat tgattgagaa agactttggt aaaggatcaa tcatgcgttt
     5461 gggtgaacgt gcggagcaaa aggtgcaagt gatgagctca ggttctttag ctcttgacat
     5521 tgcccttggc tcaggtggtt atcctaaggg acgtatcatc gaaatctatg gcccagagtc
     5581 atctggtaag acaacggttg cccttcatgc agttgcacaa gcgcaaaaag aaggtgggat
     5641 tgctgccttt atcgatgcgg aacatgccct tgatccagct tatgctgcgg cccttggtgt
     5701 caatattgac gaattgctct tgtctcaacc agactcagga gagcaaggtc ttgagattgc
     5761 gggaaaattg attgactcag gtgcagttga tcttgtcgta gtcgactcag ttgctgccct
     5821 tgttcctcgt gcggaaattg atggagatat cggagatagc catgttggtt tgcaggctcg
     5881 tatgatgagc caggccatgc gtaaacttgg cgcctctatc aataaaacca aaacaattgc
     5941 catttttatc aaccaattgc gtgaaaaagt tggagtgatg tttggaaatc cagaaacaac
     6001 accgggcgga cgtgctttga aattctatgc ttcagtccgc ttggatgttc gtggtaatac
     6061 acaaattaag ggaactggtg atcaaaaaga aaccaatgtc ggtaaagaaa ctaagattaa
     6121 ggttgtaaaa aataaggtag ctccaccgtt taaggaagcc gtagttgaaa ttatgtacgg
     6181 agaaggaatt tctaagactg gtgagctttt gaagattgca agcgatttgg atattatcaa
     6241 aaaagcaggg gcttggtatt cttacaaaga tgaaaaaatt gggcaaggtt ctgagaatgc
     6301 taagaaatac ttggcagagc acccagaaat ctttgatgaa attgataagc aagtccgttc
     6361 taaatttggc ttgattgatg gagaagaagt ttcagaacaa gatactgaaa acaaaaaaga
     6421 tgagccaaag aaagaagaag cagtgaatga agaagttacg cttgacttag gcgatgaact
     6481 tgaaatcgaa attgaagaat aagctgttaa agcagtggag aaatccgcta ctttttcgat
     6541 ttttgattca agtttttaga ttatatatag tagcttgaaa taagatatga acaact
//
LOCUS       DAVLSL010000100         6579 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLSL010000100 DAVLSL010000000
VERSION     DAVLSL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.102"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            12..454
                     /locus_tag="VXN30_001545"
                     /pseudo
     CDS             12..454
                     /locus_tag="VXN30_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826702.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            492..794
                     /locus_tag="VXN30_001546"
     CDS             492..794
                     /locus_tag="VXN30_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1358150.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            802..1603
                     /locus_tag="VXN30_001547"
                     /pseudo
     CDS             802..1603
                     /locus_tag="VXN30_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1652..2116
                     /locus_tag="VXN30_001548"
     CDS             1652..2116
                     /locus_tag="VXN30_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1358151.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2273..2698
                     /gene="lacA"
                     /locus_tag="VXN30_001549"
     CDS             2273..2698
                     /gene="lacA"
                     /locus_tag="VXN30_001549"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1358152.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2729..3244
                     /gene="lacB"
                     /locus_tag="VXN30_001550"
     CDS             2729..3244
                     /gene="lacB"
                     /locus_tag="VXN30_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1358153.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQIDANFFTEF
                     LEKWDRGEYHD"
     gene            3255..4184
                     /locus_tag="VXN30_001551"
     CDS             3255..4184
                     /locus_tag="VXN30_001551"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1358154.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4186..5166
                     /gene="lacD"
                     /locus_tag="VXN30_001552"
     CDS             4186..5166
                     /gene="lacD"
                     /locus_tag="VXN30_001552"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1358155.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5400..6236
                     /locus_tag="VXN30_001553"
     CDS             5400..6236
                     /locus_tag="VXN30_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1358156.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6272..>6579
                     /locus_tag="VXN30_001554"
     CDS             6272..>6579
                     /locus_tag="VXN30_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1358157.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     G"
BASE COUNT         2163 a         1132 c         1386 g         1898 t
ORIGIN      
        1 acaaaaacct tgtgttttgc ttagaagcgg ataatcaaga ataactcttt gatcaggtag
       61 caagcttgtt ggaggaacga gaaattgtaa ctccaactta tcgtgaagct ttgatcacgc
      121 gtgaaaagtc atttccaact ggtttagata tggaatttct aggaaaggac ttgcaaatgt
      181 aatgattcct cacacagata ttgttcataa tctagctgag aaagtggtgg ttgttcgatt
      241 agagaaaccg gtgactttcc ataatatgat tgcgccaggt aaggaagtag aagtatcctt
      301 gctcttcttt atcatcaata attcaagttc aagtcaaacg aacattcttg ctcagttgat
      361 ggactttttc acaggaaatg gacatcttga agacctatca aaaatttccg aaccagaaaa
      421 actttatgct tatatagatg aagcaatcgc ttaatcttgt ctattaaaaa ataaaatcgg
      481 aggaagtcta tatgattaaa attcttgctg cctgcggtgc aggtgttaac tcaagtcacc
      541 aaattaaaag tgctctagaa gaagaacttt caaaccgtgg ttacgatgtt cactgtgatg
      601 cagttatggt gaaagatgta aacgaagacc ttatgaaagg ttacgatatc tttacaccaa
      661 tcgctgcaac agaccttggt tttgaaccag gtatcccagt tatcgaagct gggccaatct
      721 tattccgtat cccagcaatg agcgttccag tatttgacaa tattagactt cctgcgaaac
      781 aaaatatggt atagtagttc tatgaatgat gaagcaagta aacaactaac tgatgcacga
      841 tttaagcgtc ttgttggtgt tcagcgtacc acttttgaag agatgttagc tgtattaaaa
      901 acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac
      961 cttcttatgg ccactcttca atagtgcgag aatatcgaac ttatgaacaa attgcggctg
     1021 attttggtat tcacgaaagc aactttatcc gtcggagcca atgggttgaa gtaactcttg
     1081 ttcaaagtgg tgttacggtt tcaagaactc ctctcagctc tgaggacacg gtaatgattg
     1141 atgcgacgga agtacaaatc aatcgcccta aaaaagaatt agcgaatgat tctggtaaaa
     1201 agaaatgcca cgctatgaag gctcaagcga ttgtcacaag tcaagggaga ttgtttcttt
     1261 ggatatcact gtgaactatt gtcatgatat gaagttgttc aaaatgagtc gcagaaatat
     1321 cggacaagct ggtaaaatct tggctgacag tggttatcaa gggctcatga agatatatcc
     1381 tcaagcacaa actccacgta aatccagcaa actcaagccg ctaacagttg aagataaaac
     1441 ctataaccat gcgctatcta aggagagaag caaggttgag aacatctttg ccaaagtaaa
     1501 aacgtttaaa atgatttcaa caacctatcg aaatcatcgt aaacgcttcg gattacgaat
     1561 gaatttgatt gctggcatta tcaatcatga actaggattc tagttttgca ggaagtctaa
     1621 taaagaaact agcaaggtca ggccttactt tattggtgga catccaggat ttaattgtcc
     1681 tctacttgat gatgaagtct atgaagatta ttatctagag tttgagaaag aagagacttg
     1741 ctctgttcca cgtcctttcc cagaaacagg tatgttggat ttccaagata gaagtccatg
     1801 gctagaggga caaaaagaaa tagatcttag ttatgatctt ttcagcaaag atgcagtgac
     1861 tttggatgaa ttgcaatctc gaacaattgc ccttcgttct cttaaacatg ataggggatt
     1921 gaaagtgcat tttgcagaat ttccaaacct catcatctgg tcaactttga acaagggacc
     1981 tttcattacc tttgaaccat ggtctggctt gtcaacattc cttgaagaag gagatcattt
     2041 agaagataag aagaatgttt gtctcttaga agccaatcag gttgaagaat tagggtttga
     2101 gatagaagtt ttataaaaaa gtaaaaaata aataacaaaa acaaacacaa actgttgact
     2161 tcttcaaaca atagtagtat attatgtttg tgaggactaa ttgactgttt tttaaagaca
     2221 gccaatacac tgctggaatc cagcatagaa aaaataaaaa ggagtataca atatgtctat
     2281 tgttatcggt gcagatgctg caggtttgag attgaaagaa gttgtgaaag acttcttaga
     2341 aaaagaaaac ttccaccttg tggatgttac agctgaaggt caagactttg ttgatgtgac
     2401 tctcgctgtt gctgcagaag taaacaaaga agaacaaaac cttggtatcg tgattgatgc
     2461 ttatggagct ggtccattta tggttgcaac taagatcaaa ggaatggttg ctgcagaagt
     2521 ttctgatgaa cgttcagctt atatgactcg tggccacaac aactcacgta tgatcactat
     2581 gggtgcacaa cttgttggtg atgaattggc taaaaacatc gctaaaggat ttgttaatgg
     2641 taaatacgac ggtggtcgtc accaaatccg ggttgacatg ttgaacaaaa tgggctaatt
     2701 agattatagt aagaaaggta agttaaaaat gagaattgca attggatgtg accacatcgt
     2761 aacagatgaa aaaatggcgg tttcagaatt tttgaaatca aaaggatatg aagtcattga
     2821 ctttggtaca tatgaccata cacgtactca ctacccaatc tttggtaaaa aagtagggga
     2881 agctgtaact agcggtcaag ctgatcttgg agtatgtatc tgtggtactg gtgttggtat
     2941 caacaacgct gtaaataaag ttccaggtgt tcgttctgcc ttggttcgtg atatgacaac
     3001 agccctttat gctaaagaac aattgaacgc taacgttatt ggttttggtg gtaagattac
     3061 tggtgaattg cttatgtgtg atatcatcga agctttcatc catgctgaat acaaaccaac
     3121 tgaagaaaac aaaaaattga ttgcgaaaat tgaacatgtt gaaagtcaca atgctcaaca
     3181 aatagacgca aacttcttca ctgaattcct tgaaaaatgg gatcgcggag aataccacga
     3241 ctaagaggta acctatgatt ttaacagtta caatgaaccc atccattgat atttcctatc
     3301 ccttggatga gttgaagatt gatactgtca atcgtgtggt ggatgtaacc aaaacggctg
     3361 gtggtaaggg actcaatgtt acccgagtac tttcagaatt tggcgattct gttcttgcta
     3421 ctggtttagt gggtggcaaa cttggtgagt ttttggttga acatatcgat aatcaagtaa
     3481 agaaagattt cttctcaatt cagggagaaa ctcgtaactg tatcgctatt ctccacggag
     3541 acaaccaaac agaagttctt gaaaaaggtc ctgaagtatt ggaacaggaa ggtcaagact
     3601 ttttggaaca tttcaaaaaa ctcttggagt cagttgaagt agtggccatc tcaggtagtc
     3661 tgccagctgg ccttccagtt gattactatg cgagcttggt agaacttgct aatcaagctg
     3721 gcaagcctgt agtcttggac tgctcaggtg cagcacttca ggctgttctt gaatcacccc
     3781 ataaaccaac agtcatcaaa ccaaataatg aagaattgtc tcagcttctt ggaagagaag
     3841 tttctgagga tttggatgaa ttaaaagaag tacttcaaga acctttgttt gcagggattg
     3901 aatggattat cgtttcactt ggtgccaacg gtacttttgc caaacatggt gacactttct
     3961 acaaggtaga tattcctaga attcaggtgg taaatcctgt tggatctgga gactctactg
     4021 tggcaggaat ttcttcagga cttcttcaca aagaatcgga tgcagaatta ctcatcaagg
     4081 caaatgtcct tggtatgctc aatgctcaag aaaaaatgac tggtcatgtc aacatggcca
     4141 actatcaagt tctatatgat caattaatag taaaagaggt ataaaatggc tttaacagaa
     4201 caaaaacgtg tacgcttaga aaaactttct gatgaaaatg gtatcatctc agctcttgca
     4261 tttgaccaac gtggtgcttt gaaacgcctc atggctcaac accaaacaga agaaccaact
     4321 gtggcccaaa tggaagaact taaagtcttg gtagcagatg aattgactaa atacgcttca
     4381 tctatgcttc ttgaccctga gtatggactt ccagcaacta aagctcttga tgaaaaagct
     4441 ggtcttctcc ttgcttatga aaaaacaggt tatgacacaa caagcacaaa acgcttgcca
     4501 gactgcttgg atgtctggtc tgcaaaacgt attaaagaag aaggtgcaga tgcagttaaa
     4561 ttcttgcttt actatgatgt agatagctca gacgaactca atcaagaaaa acaagcctac
     4621 atcgaacgca tcggttctga gtgtgtggct gaagatatcc cattcttcct tgaaattctt
     4681 gcttacgatg aaaaaattgc ggatgcaggt tctgtagaat acgcgaaagt aaaaccacac
     4741 aaagttatcg gcgctatgaa agtcttttca gacccacgct ttaacattga tgtcttgaaa
     4801 gttgaagttc ctgttaacat taaatatgtt gaaggcttcg ctgaaggtga agtagtttat
     4861 acacgtgaag aagcagcagc cttcttcaaa gcgcaagatg aagcaacgaa cttgccatac
     4921 atctacttga gtgctggtgt atcagctaaa ctcttccaag atactcttgt atttgctcat
     4981 gaatcaggtg caaactttaa cggagttctt tgtggccgtg caacatgggc tggatcagtt
     5041 gaagcataca tcaaagatgg tgaaacagca gctcgcgaat ggcttcgcac aactggattt
     5101 gaaaacattg atgagctcaa caaagttctt caaacaacag caacttcatg gaaagaacgt
     5161 gtgtaagaaa gtcctcctag tttaggaaca tgaatctaaa aaaatttaaa aaaagttgta
     5221 tgtaaaggtt tacaaaataa cttacttgtg ctatacttaa atcacaagtt aatacaaggt
     5281 gagtgttact aagtaatatt aggcatgatc acaggtgaat tagaaatcag ctgattttct
     5341 agttcatttg tggtcatttt ttgtactcat atacctttaa gatataaaag gaggttgaca
     5401 tgtatcgaat tctaaatcca atgaatcaca atgtctcgct tgtcagaaat gataagggag
     5461 aagaggtgat tgtaattggt aaggggattg cattcggaaa gaagaagggg gatttgattg
     5521 ctgaaaatca ggttgagaaa atctttcgga tgaagaccga agagtccaga gaaaacttta
     5581 tggctcttct caaagatgtt ccgcttgatt ttatcacagt gacctatgaa atcattgata
     5641 agctatcaaa gaaatatcat tatccgattc aagagtatct ctatgtaacc ttgacagatc
     5701 atatttactg ttcttatcaa gctctaactc aaggaaggta caaggatagt aatctgccag
     5761 atatttctgc taagtatcct gtcgcttttc aaatcgcaaa tgaagctttt gaaatttact
     5821 gtcagaagct agcagatcat tttcctgagg acgaaattat tcggattgct tatcatttca
     5881 ttaatgctga aggtgaaaat gaagtggaac ttgtggagtc gattgataag aggaaagaaa
     5941 ttctcaggaa tgttgaagaa gttttaacgg actatgcaat tcaacgaact aaaaagaata
     6001 accatttcta tgatcgcttt atgatccatt tgaattattt cttggattat ttagacagat
     6061 ctagagatga taaccaatca cttctggata tggaagatca tattaaacaa tcctatccaa
     6121 aagccttcga gattggttcc aagatctatg atgtgattac gcaacatacg ggtcttgatt
     6181 tgtataaaag tgaacgagtt tatctagttc tacatatcca acgtttattg tcataaaaat
     6241 ttatttaaaa ctatataagg agaattctat catgaataga gaagaagtaa cattgttagg
     6301 ttttgaaatc gtagcctatg ctggcgatgc tcgttcaaaa ctattggaag ccttgaaggc
     6361 tgctgaagct ggtgattttg aaaaagcgga cgctctggta gaggaagctg gtagctgtat
     6421 tgcagaggct caccacgcgc aaacaagtct attgactaag gaagcttctg gtgaggactt
     6481 agcgtatagt gtgaccatga tgcatggtca agatcacttg atgacaacta tcttgttaaa
     6541 agacctaatg caccatttaa ttgaacttta caagagagg
//
LOCUS       DAVLSL010000101         6573 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLSL010000101 DAVLSL010000000
VERSION     DAVLSL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.103"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     regulatory      23..121
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            205..996
                     /gene="thiD"
                     /locus_tag="VXN30_001555"
     CDS             205..996
                     /gene="thiD"
                     /locus_tag="VXN30_001555"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1358158.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(1206..1838)
                     /locus_tag="VXN30_001556"
     CDS             complement(1206..1838)
                     /locus_tag="VXN30_001556"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1358159.1"
                     /translation="MNREALRLYLVTSRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(1831..2634)
                     /locus_tag="VXN30_001557"
     CDS             complement(1831..2634)
                     /locus_tag="VXN30_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1358160.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(2634..3158)
                     /gene="thiW"
                     /locus_tag="VXN30_001558"
     CDS             complement(2634..3158)
                     /gene="thiW"
                     /locus_tag="VXN30_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1358161.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(3163..3855)
                     /gene="tenA"
                     /locus_tag="VXN30_001559"
     CDS             complement(3163..3855)
                     /gene="tenA"
                     /locus_tag="VXN30_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1358162.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(3866..4516)
                     /locus_tag="VXN30_001560"
     CDS             complement(3866..4516)
                     /locus_tag="VXN30_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1358163.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(4518..5903)
                     /locus_tag="VXN30_001561"
     CDS             complement(4518..5903)
                     /locus_tag="VXN30_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358164.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLNGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(5904..6464)
                     /locus_tag="VXN30_001562"
     CDS             complement(5904..6464)
                     /locus_tag="VXN30_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1358165.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         2032 a         1439 c         1308 g         1794 t
ORIGIN      
        1 tgctatacta aagaaaaaag aaaacaacca ctaggggtgc gtaaagctga gattaacgac
       61 tgttagatcc ctctgactca atctaggtaa tgctagctga tggaagtgga aatgataatg
      121 gggactagca gtcttctatt gcctttctaa aacagactag cttgttctta agaatacaaa
      181 cttcagttgg ttgggaggtt ttagatgact tatttacccg ttgctttgac cattgcaggg
      241 actgacccta gtggtggtgc tggcattatg gcagatttaa agtcattcca agcgagagat
      301 gtctatggaa tggctgttgt aaccagtctt gtcgctcaaa ataccagagg tgttcagcta
      361 atcgagcacg tttctcctca aatgttgaaa gcccaattgg agagtgtctt ttctgatatt
      421 ccacctcagg ctgtaaaaac tggaatgttg gctactactg aaatcatgga aatcatccaa
      481 ccctatctta aaaaactgga ttgtccctat gtccttgatc ctgttatggt tgctacaagt
      541 ggagatgcct tgattgactc aagtgctaga gactatctca aaacaaactt actacctcta
      601 gcaactatta ttacgccaaa tcttcctgaa gcagaagaga ttgttggttt ttcaatccat
      661 gaccccgaag acatgcagcg tgctggtcgc ctgattttaa aagaatttgg tcctcagtct
      721 gtggttatca aaggcggaca tctcaaaggt ggtgctaaag atttcctctt taccaagaat
      781 gaacaatttg tctgggaaag cccacgaatt caaacctgtc acacccatgg tactggatgt
      841 acctttgctg cagtgattac tgctgaacta gccaagggca agagtcttta ccaggcagtt
      901 gataaggcca aggcctttat cacaaaagct attcaagatg cccctcaact cggtcatggt
      961 tctggtccag tcaaccatac aacttttaaa gattaagaaa aaaaactctc tagttcccac
     1021 tttaagggaa ttagagagtt tttatactct tcgaaaatct cttcaaacta cgtcagcttc
     1081 catctgcaac ttcaaaacac tgttttgagc tgacttcgtc agtcttatct aaaacctcaa
     1141 ggcagtactt tgagcaacct gcgactagct ttctagttta ctctttgatt ttcattgagt
     1201 attaattagg aaagaatgtc atgcaacttt gttaaaaagg cttgcgtttt tgcctcaata
     1261 tcttctgctt gcatcaaatc acgtacaaca gctacaccag ctatgccagt gcccataagc
     1321 tgatcaatat tctccgaagt caagccacca atagcaacta ttggaatggc aaccgtttgg
     1381 caaattgttt tcaaggttga aatcagggtg atgggtgcat tttccttggt ggttgtcgga
     1441 aaaatggctc ctgtccccaa gtaatctgca cctgatttct ccgcttccag agctctttta
     1501 accgttttag cggtgacacc gaggattttt tcaggaccca agacttgtcg ggcaaccgaa
     1561 actgggagtt cgtcatctcc aatatgcaga ccagcagcat caaccgcaag acaaacatcc
     1621 aaccgatcat cgattatcaa gggtacctga taagcatctg ttatttcctt gacttgtttt
     1681 gccagttgat aatattgatt ggttgtgaga tttttttctc gcaattggac tatggtaacc
     1741 cctgaacggc aggccgtctc aacttttgca agaaagcttt ccacggaatc ttgatagcga
     1801 ctggttacca gatatagtct aagtgcttct ctattcataa acctctcctt tgatggtatc
     1861 tagccaattt tcatctcttc ttaggagcga aagctgattg agtacttggt aacgaaattc
     1921 ttccaatccc attccttgaa caactatttt ctcagcagcg atattgagat aagagactgc
     1981 taagcaagaa gcttcaaaac cagtctttcc ttggctgaga aaaacggctg ttaaggctcc
     2041 aaccaaatct cctgtccctg ttatccagtc taattctgca cagccatttc ccagtacagc
     2101 gacctgattt ttcgaaacga cgaggtcctt gggacctgtg actaagaaag acataccatg
     2161 ataggtctga caccagtctt tcaagacttg aagcaaatcc tccgtttctt gatctttagc
     2221 actcgcatcg accccaacgc cgtggtgctt taatccaaca agacttcgaa tttctgacat
     2281 gtttccttta aggaccgtag gtctatagtc taaaaggtct ctgactaagc tcttacgaat
     2341 ggatgaagct gttacgccaa ccgcatctac taccattggg agacaaactt gagctgcata
     2401 agaagctgct atacggattg ctttctcctt ctcagctgac aaatgcccca aattgatgaa
     2461 gagagcctga ctttgcttag taaaatcaag aacctcacgg ggatcatctg ccatgacagg
     2521 tttgcatccc agagccaaaa tcccatttgc cagcatctca caagaaatct cattggtcat
     2581 acagtgaatg agggaactag agcctatagg aaagggattt gtaaattcct gcatcagtct
     2641 atcctttcac taaagaaata tccctgcact tttttaaaga attcctgctt gattaaaaat
     2701 cgaaaggcaa taaaggaaat cgctgtacca atcaaggttg ctccgaaaaa tcgaggcgtg
     2761 tagataaacc agctaagctt agcagctgat cctgtaaaga gtaccataac aggataggaa
     2821 acaatagaac caataatacc tgttcccaca atttctccca aggcagaaaa gtaaaatttt
     2881 cgaccgtact tataaaagag acctgctaga agggctccaa aagtcgctcc tgtgagagct
     2941 aaaggcggaa tcccttgagt cgtcatacgg ataaaggctg tcactgtagc catagccaag
     3001 gcataaacag gtcccatcat gattcccgct agaatattga ctacactgga catcggtgcc
     3061 attccctcaa tccgaaagat aggtgtaagg actacatcaa gggcaatcat catagataaa
     3121 atggtcaatt tgtgaacttg tagttggtgc tttctcatgt ttctattctt ctcctttttc
     3181 taaagactgt aaatcgctct tccatgtctg gtgttggtaa gccatctccc aaaacttggc
     3241 ttccatatga acactgatgt ggaaggcatc tagcattttt tgcttatctg tctcatcact
     3301 ttctcgatag agctgattga ccagtgctcc ctcctctctg atctgttgct ctaactcatc
     3361 cgtaatataa gtttcaatcc attgttgata gagaggattt ggtgatggtt taagattaag
     3421 tgatttgcct atatcatggt ataaccaagg acaaggaagc aagcttgcaa aagcgatggc
     3481 taagttcggt tctgcaaatt gcctataaat atgagaaatg taatgataac aggttggagc
     3541 gattggatgt tgctccattt cctggtcgct gatttccaat tccttgaaaa attgttggcg
     3601 aataaataac tcaccctcca ctaaaccctg agcattttgt ttcaagagtc ttttcatctc
     3661 ttggtttgaa gtcttatcag ccaaaagatg atagatttct gagaaagcct tcagatagta
     3721 ggcatcctga atcaggtaat agcggaaaat ggcaggttct aaattcccct cttgtaattg
     3781 taaaataaag ggatgatgaa aggaagcctg ccaagctttc ttggataatt ccatcgcaat
     3841 atctgtaaat tccataataa ctcctttata aaaatagact ggtttgaagc aataaaaaga
     3901 aaagcaggta gattaatttt gtttttttag gaatataaaa agtccgatag ctattcttca
     3961 actgtgcatg ttcgtcatat ccgtgagcag atagagctct caggtaaaga tggcgccacc
     4021 taaagactgt catcagaacc ttactgtaaa tcaagggcga ccaaaaatgt agttcttgac
     4081 cacgtaatag gcaagcttct ttgagggact tgatttcttg ctgaatgaga ggaaaagaat
     4141 tgaataccac aatcaaggca taggaccaag agcgtgatag ccccttttga gccaagtaca
     4201 agagaagctc ttttagtgaa atagaggaaa caaagacaag gccgatacaa actgtcacaa
     4261 aggccctcgt tccaagcatg actgcctgtg aagcatctcc gtgtaactga actgcccagt
     4321 agttggcaaa agatggtaaa atggcaagta tgatcatcca agctaacatt ttaaatcgac
     4381 ggtaatagag cataaagaga atacaaaatg cgactaccga aagattcaga gcaatcgaag
     4441 gaatgaaaga tgtttccaag gataaaatca gcaagaagag actgataatc ggtgtctggg
     4501 ttgctacttt gaccatacta tctcacctcc ccttgggtat tgctactctg agatgtaagt
     4561 ggtttggtaa tggtcacttc tttcacatgc cgaagaccct gactagtcat ctcaatccaa
     4621 taatcaacca cagaaatcaa agggtctaaa cgatgactaa tgagcagaaa acttcttcct
     4681 tgattcctct cctccacaat ccacttgcaa aaataatggc aggctctatc atccaaacct
     4741 gcaaaaggtt catctagcaa gatcacggaa gccttactgg tcaagatggt caggagctga
     4801 agaatttttt gctgaccacc atttaattga tagggactct tatcgactgc ctgctccaaa
     4861 tcaaaatatc gtaaagcttg aaaaatccgc tgatttcttt cagaatcagg tccatctaat
     4921 tgaagctcct ctcgcagact gactcggata aactgcttct cagcttcctg aacaacacca
     4981 gtcagatcac gatacaaact ctttttcttt ttcaggaccg aacccttcca tgcaatgcgc
     5041 cccttatact tttgaaattg aagaatagac cgaaagaggg ttgatttccc gacaccattg
     5101 tcacccagga tacaggaaat cccttgatag aatgtgaaat cagcaattga aaagaggggg
     5161 cgatttccaa actcacaagt cgttctatcc atatggaata gttccgggct agaagcaact
     5221 tcctttgaag caacctgtgt catctcatag gaagggattt gaaacacttc ccttagtttt
     5281 ccgtctctta gctcaaccat atggtcgata taggctttat agtcagataa atcatggtcg
     5341 cacaaaataa ctgtctttcc atcaagagcc aactctttta gaatctccaa tatctcgatt
     5401 ctgctcttgc ggtcaatgga agcaaaaggt tcatccaaga gatagaccct aggattcatg
     5461 gcaaagagga cagccagcgc tgctttttgc ttttccccac ctgataggtg atgaatggga
     5521 cggtgtaaga ttgcctcgca acgacattgc tggacaacct ctgctatttt agaatcaatt
     5581 tcctgaaggt gatagccgat attctccaag gtgaaaatca gctcctcaaa caagctctcc
     5641 atggtaaatt gatgattggg attttgaaag agaatgccaa ccttctggac acgtgcgatg
     5701 atagaaagtt gactgacctc gctcccatct atcaggactt gaccactata gggaagagaa
     5761 ctgacttggg caatcatttg aaagaggctg gattttccag acccactact cccaactaac
     5821 aaggtaaagg cttgcgcatg aaaagtaaaa tcaaacggct cagagaagat tggggactga
     5881 atcgctcgta gttccagacc catctatgct tttcctccag ttgcaaactg atgatagagt
     5941 ttgacaatgg cacgaaccaa gatggtacag aagaaataaa cagaaataaa acgtaccaca
     6001 agtaaggaaa ggacaaacgg aagggaaaag gcgtagtaac ctaacttaat gtattcatag
     6061 acaaagctaa caagcgtaat cccaatacta ttagcagtta gagagagcca actttcatag
     6121 cgattcttag ttacgataaa accaaattca cttcccaaac cttgaaccaa gccagacaaa
     6181 agagctccta gaccaaattg gctaccataa aggacttcag caagcgcagc tagcacttct
     6241 ccaatcgttg cacttccgac tctcggaaca aagatggcag caatgggcgc agccatacac
     6301 cagagaccga agaggatttc attggcaaag gcctgcaaac caagaggtgt taagagtaga
     6361 ctgagaatat tatacacata tcctgaacca acgaaaaccc caccaaaaaa gatagacaag
     6421 aaagcaagca agataacatc ttttaactgc cattttttca acataaaaaa ctcctttttt
     6481 taaagaaaag tggggcactc aagaagaccg acctaaatac tttgtatagc agactgaatt
     6541 tagaacagta cacaagaatg ctaaaatatt tct
//
LOCUS       DAVLSL010000102         6524 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLSL010000102 DAVLSL010000000
VERSION     DAVLSL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.104"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            217..480
                     /locus_tag="VXN30_001563"
     CDS             217..480
                     /locus_tag="VXN30_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1358166.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            486..2219
                     /gene="ptsP"
                     /locus_tag="VXN30_001564"
     CDS             486..2219
                     /gene="ptsP"
                     /locus_tag="VXN30_001564"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1358167.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2474..4924
                     /gene="phtA"
                     /locus_tag="VXN30_001565"
     CDS             2474..4924
                     /gene="phtA"
                     /locus_tag="VXN30_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1358168.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            5082..>6524
                     /gene="phtB"
                     /locus_tag="VXN30_001566"
     CDS             5082..>6524
                     /gene="phtB"
                     /locus_tag="VXN30_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1358169.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPSPQPAPSNPIDEKLVKEAVRKVGDGYV
                     FEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDL
                     LARIHQDLLDNKGRQVDFEALDNLLERLKDV"
BASE COUNT         2197 a         1241 c         1371 g         1715 t
ORIGIN      
        1 tgaaatagca atgattatcc tataaattgt ttttgaagaa actatatcct gtgttttgtt
       61 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
      121 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
      181 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
      241 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
      301 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
      361 ggtgttatga gtcttggtgt tggccaaggt gctgacgtaa ctatctcagc tgaaggtgca
      421 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
      481 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
      541 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
      601 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
      661 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
      721 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
      781 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
      841 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
      901 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctatcaat gaagaagtga
      961 ttgtgattgc gcatgacttg acaccatctg atacagctca attggacaaa aactttgtaa
     1021 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
     1081 ttgaaattgc tgctgtatta ggtacaaata acatcactga aatcgttaaa gacggtgaca
     1141 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
     1201 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
     1261 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
     1321 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
     1381 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
     1441 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
     1501 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
     1561 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
     1621 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
     1681 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
     1741 ctgaaggtgt tgcagttgcg gataacatcc aagttggtat catgatcgag attcctgcag
     1801 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaacg
     1861 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
     1921 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
     1981 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
     2041 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
     2101 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
     2161 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
     2221 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
     2281 agaaaatctt tcttataaaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
     2341 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
     2401 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
     2461 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
     2521 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
     2581 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
     2641 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
     2701 aggctatgtc acttcacatg gcgaccacta tcattattac aatggtaagg ttccttatga
     2761 tgcgattttc agtgaagaat tactcatgaa agatccaaac tataagctaa aagatgagga
     2821 tattgttaat gaggtcaagg gtggatatgt tatcaaggta gatggaaaat actatgttta
     2881 ccttaaggat gctgcccacg cggataacgt ccgtacaaaa gaggaaatca atcgacaaaa
     2941 acaagaacat agtcaacatc gtgaaggtgg gactccaaga aacgatggtg ctgttgcctt
     3001 ggcacgttcg caaggacgct atactacaga tgatggttat atctttaatg cttctgatat
     3061 catagaggat actggtgatg cttatatcgt tcctcatgga gatcattacc attacattcc
     3121 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcgggg
     3181 aaatctgtca aattcaagaa cctatcgccg acaaaatagc gataacactt caagaacaaa
     3241 ctgggtacct tctgtaagca atccaggaac tacaaatact aacacaagca acaacagcaa
     3301 cactaacagt caagcaagtc aaagtaatga cattgatagt ctcttgaaac agctctacaa
     3361 actgcctttg agtcaacgac atgtagaatc tgatggcctt gttttcgacc cagcgcaaat
     3421 cacaagtcga acagctagag gtgttgcagt gccacacgga gatcattacc acttcatccc
     3481 ttactctcaa atgtctgaat tggaagaacg aatcgctcgt attattcccc ttcgttatcg
     3541 ttcaaaccat tgggtaccag attcaaggcc agaacaacca agtccacaac cgactccgga
     3601 acctagtcca ggcccgcaac ctgcaccaaa tcttaaaata gactcaaatt cttctttggt
     3661 tagtcagctg gtacgaaaag ttggggaagg atatgtattc gaagaaaagg gcatctctcg
     3721 ttatgtcttt gcgaaagatt taccatctga aactgttaaa aatcttgaaa gtaagttatc
     3781 aaaacaagag agtgtttcac acactttaac tgctaaaaaa gaaaatgttg ctcctcgtga
     3841 ccaagaattt tatgataaag catataatct gttaactgag gctcataaag ccttgtttga
     3901 aaataagggt cgtaattctg atttccaagc cttagacaaa ttattagaac gcttgaatga
     3961 tgaatcgact aataaagaaa aattggtaga tgatttattg gcattcctag caccaattac
     4021 ccatccagag cgacttggca agccaaattc tcaaattgag tatactgaag acgaagttcg
     4081 tattgctcaa ttagctgata agtatacaac gtcagatggt tacatttttg atgaacatga
     4141 tataatcagt gatgaaggag atgcatatgt aacgcctcat atgggccata gtcactggat
     4201 tggaaaagat agcctttctg ataaggaaaa agttgcagct caagcctata ctaaagaaaa
     4261 aggtatccta cctccatctc cagacgcaga tgttaaagca aatccaactg gagatagtgc
     4321 agcagctatt tacaatcgtg tgaaagggga aaaacgaatt ccactcgttc gacttccata
     4381 tatggttgag catacagttg aggttaaaaa cggtaatttg attattcctc ataaggatca
     4441 ttaccataat attaaatttg cttggtttga tgatcacaca tacaaagctc caaatggcta
     4501 taccttggaa gatttgtttg cgacgattaa gtactacgta gaacaccctg acgaacgtcc
     4561 acattctaat gatggatggg gcaatgccag tgagcatgtg ttaggcaaga aagaccacag
     4621 tgaagatcca aataagaact tcaaagcgga tgaagagcca gtagaggaaa cacctgctga
     4681 gccagaagtc cctcaagtag agactgaaaa agtagaagcc caactcaaag aagcagaagt
     4741 tttgcttgca aaagtaacgg attctagtct gaaagccaat gcaacagaaa ctctagctgg
     4801 tttacgaaat aatttgactc ttcaaattat ggataacaat agtatcatgg cagaagcaga
     4861 aaaattactt gcgttgttaa aaggaagtaa tccttcatct gtaagtaagg aaaaaataaa
     4921 ctaatgaaaa atgaaagtct cgataaagag gctttcattt ttattatgta tatatgtaaa
     4981 attcttgaca agcaatatta aaaagagtaa actattaact agttaattaa ccggtttatt
     5041 actttatagt gaatcaaata tacttaagaa aagaggaaag aatgaaaatc aataaaaaat
     5101 atctagcagg ttcagtggca gtccttgccc taagtgtttg ttcctatgag cttggacgtt
     5161 accaagctgg tcaggataag aaagagtcta atcgagttgc ttatatagat ggtgatcagg
     5221 ctggtcaaaa ggcagaaaac ttgacaccag atgaagtcag taagagggag gggatcaacg
     5281 ccgaacaaat tgttatcaag attacggatc aaggttatgt gacctctcat ggagaccatt
     5341 atcattacta taatggcaag gttccttatg atgccatcat cagtgaagag ctcctcatga
     5401 aagatccgaa ttatcagttg aaggattcag acattgtcaa tgaaatcaag ggtggttatg
     5461 tcattaaggt aaacggtaaa tactatgttt accttaagga tgcagctcat gcggataata
     5521 ttcggacaaa agaagagatt aaacgtcaga agcaggaacg cagtcataat cataactcaa
     5581 gagcagataa tgctgttgct gcagccagag cccaaggacg ttatacaacg gatgatgggt
     5641 atatcttcaa tgcatctgat atcattgagg acacgggtga tgcttatatc gttcctcacg
     5701 gcgaccatta ccattacatt cctaagaatg agttatcagc tagcgagtta gctgctgcag
     5761 aagcctattg gaatgggaag cagggatctc gtccttcttc aagttctagt tataatgcaa
     5821 atccagctca accaagattg tcagagaacc acaatctgac tgtcactcca acttatcatc
     5881 aaaatcaagg ggaaaacatt tcaagccttt tacgtgaatt gtatgctaaa cccttatcag
     5941 aacgccatgt ggaatctgat ggccttattt tcgacccagc gcaaatcaca agtcgaaccg
     6001 ccagaggtgt agctgtccct catggtaacc attaccactt tatcccttat gaacaaatgt
     6061 ctgaattgga aaaacgaatt gctcgtatta ttccccttcg ttatcgttca aaccattggg
     6121 taccagattc aagaccagaa caaccaagtc cacaaccgac tccagaacct agtccaagtc
     6181 cgcaaccagc tccaagcaat ccaattgatg agaaattggt caaagaagct gttcgaaaag
     6241 taggcgatgg ttatgtcttt gaggagaatg gagtttctcg ttatatccca gccaaggatc
     6301 tttcagcaga aacagcagca ggcattgata gcaaactggc caagcaggaa agtttatctc
     6361 ataagctagg agctaagaaa actgacctcc catctagtga tagagaattt tacaataagg
     6421 cttatgactt actagcaaga attcaccaag atttacttga taataaaggt cgacaagttg
     6481 attttgaggc tttggataac ctgttggaac gactcaagga tgtc
//
LOCUS       DAVLSL010000103         6505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLSL010000103 DAVLSL010000000
VERSION     DAVLSL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.105"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            169..1119
                     /locus_tag="VXN30_001567"
     CDS             169..1119
                     /locus_tag="VXN30_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1358170.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1296..2303
                     /gene="gap"
                     /locus_tag="VXN30_001568"
     CDS             1296..2303
                     /gene="gap"
                     /locus_tag="VXN30_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1358171.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2371..3207)
                     /locus_tag="VXN30_001569"
     CDS             complement(2371..3207)
                     /locus_tag="VXN30_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1358172.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3333..5528
                     /locus_tag="VXN30_001570"
     CDS             3333..5528
                     /locus_tag="VXN30_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1358173.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5581..5733
                     /gene="rpmG"
                     /locus_tag="VXN30_001571"
     CDS             5581..5733
                     /gene="rpmG"
                     /locus_tag="VXN30_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1358174.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5743..5919
                     /gene="secE"
                     /locus_tag="VXN30_001572"
     CDS             5743..5919
                     /gene="secE"
                     /locus_tag="VXN30_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1358175.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5974..>6505
                     /gene="nusG"
                     /locus_tag="VXN30_001573"
     CDS             5974..>6505
                     /gene="nusG"
                     /locus_tag="VXN30_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1358176.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2025 a         1133 c         1410 g         1937 t
ORIGIN      
        1 atatcaaaaa gcacctcaaa aaggtattac caagttgcaa taccttttat gaggctcttt
       61 tgtcttgttc ttgtttcaat tgactatagt tcacggatac agttgggaaa gaagctatta
      121 aatatttgga gagtcctttt gggatgaaca tgtcaaatat tactatagat gggcaagaaa
      181 agatgggcaa gaaatggatc tgagtcaaac gatgcttctt atgctcaagt agtgagtctt
      241 tatgatgata caagtatctc tgttagtaat aatgagacgg acaaagtgtt gactggatcc
      301 ctctatactg aaacaaatga acagggatta acgattccaa gcagtttact aaaaaattgg
      361 aatgaacaga caggaaaaaa tttgacaact aatgatctta ttggcaaatc agtctcagcc
      421 agcattgtag aaagtgctgc cgaaactagt aagattgctc aatttcaaac gaagattgta
      481 cgtgtaatca atgatgaaga tgacatggag gacagcaaca gtttcatgct gtctcatcaa
      541 atggaaacga ttttgaaaga ggctggattt acgaaagctg tatcttattt tatcttggaa
      601 ctcaaagatc catcacagac aaaagtagta acagaagaat tacagaaaaa taagaagtat
      661 actgtgcttt ctcaacagag agttcttgat attgtgatta cctttattcg tgttattcag
      721 ggattattga ttgtgctttc atcacaagct attgtggtag cggcggttat gattggtatc
      781 attatttaca tcaatatcat gcaacgttcc aaggaaatag gtgtcatgaa agcagttggt
      841 tatcagaatc gtggtgtcaa aggaattttt atttacgagg ctatctggat tgtaggcatc
      901 gccttgctgc tggcattttt ggttgcacaa ggggtgggaa gtttggcgaa tgcgattgta
      961 agtcactttt acccatccat cactaaggtt tttgaattaa atcttttatc tgttttagga
     1021 actctagttt tcgctctatt acttggttat gtctcagcct acttcccggc gcgtaagatt
     1081 agtaaaatgg atcctgtaga atcgttacgc tatgaataat aagggattcc ttgatttaca
     1141 gtcttaaaaa gaaactcaca tagaaaaaca atggccatac ttgcaattca actcaaatag
     1201 ttgtataata ggtttgttga aaggtgattt gtagtgattc aagttactct tttcacaata
     1261 aaaatttcat gattttcata aggaggaaat cactaatggt agttaaagtt ggtattaacg
     1321 gtttcggacg tatcggtcgt cttgctttcc gtcgtatcca aaacgtagaa ggtgttgaag
     1381 ttacacgcat caacgacctt acagatccag ttatgcttgc acacttgttg aaatacgaca
     1441 caactcaagg tcgtttcgac ggtactgttg aagttaaaga aggtggattc gaagttaacg
     1501 gtaaattcgt taaagtttct gctgaacgtg atccagagca aatcgactgg gctactgacg
     1561 gtgtagaaat cgttcttgaa gctactggtt tctttgctaa gaaagaagca gctgaaaaac
     1621 accttaaagg tggagctaaa aaagttgtta tcactgctcc tggtggaaac gacgttaaaa
     1681 cagttgtatt caacactaac cacgacgttc ttgacggtac tgaaacagtt atctcaggtg
     1741 cttcatgtac tacaaactgc ttggctccaa tggctaaagc tcttcaagat aactttggtg
     1801 ttgttgaagg attgatgact actatccacg cttacactgg tgaccaaatg atccttgacg
     1861 gaccacaccg tggtggtgac cttcgccgtg ctcgcgctgg tgctgcaaac atcgttccta
     1921 actcaactgg tgctgcaaaa gctatcggtc ttgtaatccc agaattgaat ggtaaacttg
     1981 atggatctgc acaacgcgtt ccaactccaa ctggatcagt tactgaattg gtagcagttc
     2041 ttgaaaagaa cgttactgtt gatgaagtga acgcagctat gaaagcagct tcaaacgaat
     2101 catacggtta cacagaagat ccaatcgtat cttcagatat cgtaggtatg tcttacggtt
     2161 cattgtttga cgcaactcaa actaaagttc ttgacgttga cggtaaacaa ttggttaaag
     2221 ttgtatcatg gtacgacaac gaaatgtcat acactgcaca acttgttcgt actcttgaat
     2281 acttcgcaaa gattgctaaa taattcttga gttgatagaa agcaaggctt tgtggtcttg
     2341 cttttttata tggaaaaatg gatgacacga tcatccattc ttttttaatt ctttttcaaa
     2401 tgtatttgaa agggtagtga aagttagcct ctctaaagta agtgggtggg taaaggaaag
     2461 tcggaaggca tgaagcataa gccggcttgt ctttgattta ctattataga gagggtctcc
     2521 caggatagga agattatgat gcaaaaggtg cacacgaatc tgatgggttc gccctgtctt
     2581 tagcttgcaa tgagccaagg aagtcttgtt tgagaattgc tttaatctgc ttacatgcgt
     2641 ttcagcatat ttcccatttt ttgcatcaac tattcttttt ctacgatcat ggcgatcacg
     2701 tccaattttg tctctgaaaa caagttcttt tctgttgata tttccatcaa ctagagccca
     2761 atattctcta gaaatctctt ttttctccaa taagcgattg agaatgggca ggataaaagg
     2821 atttttggca aagagaacta agccactggt ttccatgtcc agacgatgaa cgacatagca
     2881 ggtttggcca acataggtac tgacatggtt aagaagggca atttcgtttg gttgattacc
     2941 atgcgttttc atcccctctg gtttgtttac aataatcaag tgttgatctt gataaacttc
     3001 ctgcactaag tctgggttgc cccaagggat cgtcttttgg ggataatctt cctcgtcaaa
     3061 agtcaactgg caaacatctc caggatttac gatttcgttc cagcggactt cttcttgatt
     3121 tatcaaaata tgtttcttga ttctcaaaaa atgacggatt tttctaggga tgaggagttg
     3181 ttcctcaagt aattgcttta ccgtcatttg aggtagagag gcgggtaatg taaatgtgaa
     3241 ttgcatacag atattgtaac aaaaaaagcc ctatttggat aggaaatagc taaattcttg
     3301 tcttcctatg atgaagatga taaaataaac gcatgaaatt agataaatta tttgagaaat
     3361 ttctttctct ttttaaaaaa gaaacaagtg aactagagga ctctgattct actatcttac
     3421 gtcgctctcg tagtgatcga aaaaaattag cccaagtagg tccgattcga aaattctggc
     3481 gtcgttatca tctaacaaag attatcctta tactaggttt gagtgcaggc ttgctagttg
     3541 gaatctattt gtttgctgta gccaagtcga ccaatgtcaa tgatttgcaa aatgccttga
     3601 aaactcggac tcttattttt gaccgtgaag aaaaagaggc tggtgccttg tctggtcaaa
     3661 agggaaccta tgttgagctg actgacatca gtaaaaactt gcagaatgct gttattgcga
     3721 cagaagaccg ttctttctat aaaaatgacg ggattaacta tggccgtttc ttcttggcta
     3781 ttgtcactgc tggacgttca ggtggtggct ctaccattac ccaacagctg gctaaaaacg
     3841 cctatttatc gcaggatcaa actgttgaga gaaaagcgaa agaatttttc cttgccttag
     3901 aattaagcaa aaaatatagt aaggagcaaa ttctaaccat gtaccttaac aacgcttatt
     3961 ttggaaatgg tgtgtggggt gtagaagatg cgagtaagaa atactttgga gtttctgcat
     4021 cagaagtgag tctggatcaa gctgcgactc tggcagggat gctcaagggg ccggaactgt
     4081 ataatccctt gaattccgta gaagattcta ctaatcggcg cgatactgtc ttgcagaata
     4141 tggttgcagc aggatatatt gataaaaacc aagaaaccga agctgctgaa gttgatatga
     4201 cttcgcaatt gcacgataag tatgaaggaa aaatctcaga ttaccgttac ccctcttatt
     4261 ttgatgcggt ggttaatgaa gctgtttcca agtataatct aacagaggaa gagattgtca
     4321 ataatggcta ccgcatttac acagagctgg accaaaacta ccaagcaaat atgcagattg
     4381 tttatgaaaa cacatcgcta tttccgaggg cagaggatgg aacgtttgct caatcaggaa
     4441 gtgtagctct cgaaccgaaa acagggggag ttcgtggagt tgtcggtcaa gttgctgaca
     4501 atgataaaac tggattccgg aatttcaact atgcaaccca atcaaagcgt agtcctggtt
     4561 ctacaattaa gcctttagtt gtttatacac cagcagttga agcaggctgg gctttgaata
     4621 agcagttgga taaccatacc atgcagtatg atagctataa ggttgataac tatgcaggga
     4681 tcaaaacaag tcgagaagtt cctatgtatc aatccttggc agaatcgctt aatctacctg
     4741 ctgttgccac tgttaatgat ttgggtgttg acaaggcttt tgaggcaggc gaaaaattcg
     4801 gactcaacat ggaaaaggtc gaccgtgttc ttggtgtcgc cttgggaagc ggtgttgaaa
     4861 ccaaccctct tcaaatggct caagcatacg ctgcctttgc aaatgaaggt ttaatgcctg
     4921 aagctcattt tattagtaga attgaaaatg ctagtggaca agttattgcg agtcataaaa
     4981 attcacaaaa acgggtgatt gataagtctg tagctgacaa gatgaccagt atgatgttgg
     5041 ggactttcac caacggtacc ggtattagtt catcgcctgc agactatgtc atggcaggga
     5101 aaactggaac aactgaagca gttttcaatc cggagtacac aagtgaccag tgggtaattg
     5161 gttatactcc ggatgtagtg attagccact ggcttggctt tccgaccact gatgaaaatc
     5221 actatctagc tggctctact tcaaacggtg cagctcatgt ctttagaaac attgccaata
     5281 ccattttacc ttatacgcca ggaagtacct ttacggttga aaatgcttat aagcaaaatg
     5341 gaattgcacc agccaataca aaaagacaag tacaaaccaa tgataatagc cagacagatg
     5401 ataatttgtc tgatattcga gggcgtgcgc aaagtctagt agatgaggct agccgggcta
     5461 tctcagatgc gaagattaag gaaaaggctc aaacaatatg ggattcgata gtcaatctat
     5521 ttcgctaaga tgcttgtcaa agcctagctt tcttgttata atggataaga tggaggcgtt
     5581 atggcactaa aaaaagcaag cctagcttgt gcggtttgtg gttcgagaaa ctattcaatc
     5641 aagatcagcg gaaaccccaa gcctacacga ctagaagtaa ataaattttg taagcattgt
     5701 ggcaagtaca ctacacacag agaaacgaga taggagagag cgatgcgttt tattggagat
     5761 atttttagac ttcttaaaga cacaacatgg ccaactcgca aggaaagctg gagagatttt
     5821 cgttctatca tggaatacac agctttcttt gtagtaatta tttacatttt tgaccagttg
     5881 attgtttcag gtttgattcg atttattaac attttttaga agattagtgg agttaattac
     5941 actagaaatc ttctatttat gaaaggaaat atcatggata gttttgataa aggatggttt
     6001 gttttacaaa cttattctgg ttatgaaaat aaggtgaaag aaaatctatt acaacgtgca
     6061 caaacctaca atatgttgga taatattcta cgcgttgaaa ttccaacaca aacagtgcaa
     6121 gttgaaaaaa atggaaagag aaaagaagta gaagaaaatc gctttccagg ttatgttctt
     6181 gtagaaatgg tcatgacaga tgaagcttgg tttgttgttc gaaacacacc aaatgttaca
     6241 ggatttgtcg gatcacacgg gaacagatca aaaccaactc cattattgga acaagaaatt
     6301 cgtgacattt tggtatctat gggacaaact gttcaagaat ttgatttcga tgttgagatt
     6361 ggtcaaactg tacgtattat tgatggtgct tttgcagact acactggtaa gattacagaa
     6421 attgataata acaaagtgaa aatgattatc tctatgtttg gtaatgacac agttgcagaa
     6481 gtaaacctaa accaaatcgc agaat
//
LOCUS       DAVLSL010000104         6474 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLSL010000104 DAVLSL010000000
VERSION     DAVLSL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.106"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(46..312)
                     /locus_tag="VXN30_001574"
     CDS             complement(46..312)
                     /locus_tag="VXN30_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358177.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(438..885)
                     /locus_tag="VXN30_001575"
                     /pseudo
     CDS             complement(438..885)
                     /locus_tag="VXN30_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1041..2042
                     /locus_tag="VXN30_001576"
     CDS             1041..2042
                     /locus_tag="VXN30_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1358178.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            complement(2110..4512)
                     /locus_tag="VXN30_001577"
     CDS             complement(2110..4512)
                     /locus_tag="VXN30_001577"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1358179.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4589..5098)
                     /locus_tag="VXN30_001578"
     CDS             complement(4589..5098)
                     /locus_tag="VXN30_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358180.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(5098..6144)
                     /gene="pheS"
                     /locus_tag="VXN30_001579"
     CDS             complement(5098..6144)
                     /gene="pheS"
                     /locus_tag="VXN30_001579"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1358181.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT         1795 a         1490 c         1209 g         1980 t
ORIGIN      
        1 aagtgactat gacctgttat tagaaaagac tataactgat tctagtcaac tttttccctg
       61 ttcaagtggg acgattgcta gtgtctttcc taaactggct aggactttta agactgtatc
      121 caactgagga ctagtctttc ctgtctccat cctagctatg acaggctggc ttattccact
      181 gacttcttcc agctttttct gactgattcc ttgttcatac ctagcctcaa tcaactcgct
      241 catgatagcc actcgcatat cactttcaag gatttcctcc ttgctaaaga gctcagatgg
      301 acatccttcc aattactccc aatagcacta ttcttcatca cttaaccctc tttcttttac
      361 gtctatgtat tttaaaaaaa atgagcgaat tatgattcga tagattgacc agcgggttta
      421 aagttggtgc tagcctattt cttaagcgat tttccttttc taggataaag cagatcctgc
      481 ttgcttaacc ccaattttcc acgatgaatc caatagtaaa tggttgaaat tcccacgtta
      541 acccctttag ccatcaccat catttcaggc gaaaattttt ggttatgtat agtggagaat
      601 cttttccttt agttccttgg tcaagcttga tttcttgacc gagcgcttgc gattgttttc
      661 ataagactgt tgagcgtagt cggcagaata aacctctttg aagcgccctt ttccaagaca
      721 ttttcggact gtcccacgct tgatttcagt gtggatagtt tgaggagctt ttccaagtag
      781 agaggcaact tctctatttg attttccttc ttttttccat ctttcgatta agcgacggct
      841 atcgattgtc aaatgtttac cttttgtagt ataattgtct tgcatctctg tgccttttaa
      901 tcatttcaat cttaaattgg acttttttta cttgggttgt acttaatcta taaggaagac
      961 aagaaaaaga atatcaatca agtaaagtca caaagtcaca ttagctccga gcaaccattg
     1021 caaattgagg tactcacaca atgattaaaa catttctctc tgccctttcg gtcattctct
     1081 ttgttatccc tatcataact tattcttttt ttccatcttc taatcttaat atttggctat
     1141 ctaccagacc tatcttggca cagatttatg ccttcccctt agctactgca actatggctg
     1201 ccattttaag tttcgtattt ttttccctat ctttttacaa gaaaaataaa caaatacggt
     1261 tttactctgg cattttgctc ttactatcgc tcatattact attattcgga acagataaaa
     1321 ccctttcttc tgcatcaaat aagactaaaa ccttaaaatt agtaacttgg aacgtcgcta
     1381 atcaaataga agcacaacat attgagcgaa tttttagcca ttttgatgcc gatatggcta
     1441 tattccctga actagctacc aatatcagag gtgagcaaga aaaccagaga atcaaactat
     1501 tgtttcatca agttggactt tctatggcca actatgatat tttcacttct ccacctacca
     1561 atagtggaat agctcctgtg actgtgattg tcaagaaaag ttatggtttc tatacagaag
     1621 ctaaaacttt tcatacaaca cggttcggga caattgtatt acattcgaga aaacaaaata
     1681 taccagatat cattgccttg catactgctc ctcctctgcc aggtttaatg gaaatctgga
     1741 agcaagactt aaacatcatt cataatcaat tggcttcaaa atatccaaag gctattattg
     1801 caggtgattt taatgcaact atgcgtcatg gagcacttgc aaaaataagc tctcataggg
     1861 acgcattgaa tgcacttcca ccttttgaaa gaggaacttg gaatagccaa agtccaaaac
     1921 tttttaatgc aacgatagat catatcttat tgcctaaaaa ccactactat gttagagatt
     1981 tagacattgt aagttttcaa aactctgatc atagatgtat ttttacagaa atcacatttt
     2041 aattatttta tataaaatca cccctctagt gttcataaac tagaggggga atttgtatca
     2101 tactatcgtt taacgcactt ctgcattgac tttttcttcg agcgatgctt ggattttttc
     2161 catatagcgt gcgacttctt cgtccgttaa gctatcttct ggattttgga aggtcaagct
     2221 ataagccatt gacttcatac caagtcccaa tttctcacct gagaagacgt caaagagttt
     2281 gatatctgtc aaacgtttca cgccggcagc ttggatagca tctacaactt cttgatgagt
     2341 cacttctgcc ttgaggagaa gggcaacgtc acggctgact gccgggaatt tggtgatttc
     2401 tacaaatgga gtcgctggct gaagcgcagc ttcgatagct gaaaggttaa gctcagccac
     2461 atacgtttct ggaatatcgt aagccttggc agtgactgga tgcacttggc caaggaaacc
     2521 aagaacttgg tcaccgaatg aaatcacggc tgtacgacct ggatgaaggc tagcgatttc
     2581 agatgttgct gtataggtta cttggagtcc caaacgagta aatagggctt caaggattcc
     2641 cttagcatag aagaaatcaa ctggaactgc tgctgtttgg aaatcttttt cagcaaccaa
     2701 gcctgtcaag gcaaaggcaa aactgttgat ttcatttgga agttcttctt ttggattacc
     2761 tgtttgttca aagacttttc caatctcgta aagggccaag tttttattct tacgagccac
     2821 gttgtaggca acggtatcaa ggatacctga aatcatattt tgacggagga ctgaacgatc
     2881 cacagtcatt ggccacatga gttccgtaag gttacttggt tgagccgtaa actcaactgc
     2941 tttttcagga gttgttagag tataggtgat gatttctgtc aaacctgctc cttcagcaat
     3001 agtacgaact tgacggcgga gtttttgtgt ggctgtcaat tcacctgctg taccatcgtc
     3061 ttttggtaga ctagttggca agcggtcata accatagata cgagcaattt cttcaaagag
     3121 gtcagcttcg attgtgatat cccaacgacg acgtgggact ctgactgtaa agctgtctgc
     3181 atttccagaa agaccaaagc caagacgacg gaagacgtct tctacatcag cataagacag
     3241 ctcagttccg aggacacgat taacatcagc aagggttgaa gaaacttcta catctgaagt
     3301 atcaagctca cccgctgaaa cgatgccctt acgcaccgtc gcacctgcaa gttccgcaat
     3361 caggctagct gccgcatcaa gggcttcatt aactgttgcc acattaattc ctttttcaaa
     3421 gcgagaagat gactcagaac gaaggttcag gcgaccactt gtcttacgga tagatttgcc
     3481 attgaaaaca gcagcttcaa ggacaacacg actagatttt tcagagattt ctgttgcttg
     3541 accacccatg acacctgcaa gggctactgg cttgtctgcg acagtgatga ctaggtcatt
     3601 cacgtccaag tcacgttctt caccgtccaa ggtcaccaat ttttcaccag cacgcgcttc
     3661 acgcacacgg atgtcagtcc cttcaaagtt atccaagtca aaggcatgca ttggttgacc
     3721 aaaatagagc aggatgtagt tggtcacgtc cactacgtta ttgatgggac ggattccttc
     3781 gttcatgaga agattttgca accattgtgg acttggtgcg atggtcacat tgtccaagat
     3841 acgagctgca tagtaaggcg ccttgtctgt ctcaatgctg acagaaaggg catctgccgc
     3901 agcttcatta gtttctgtta gagtaaattc tttaaagttg actgccttgt catagatggc
     3961 tgccacttcg tgagccactc cacacataga aagggcatct gcacggtttg gtgtgatgga
     4021 aagttcgatg atttcatcat ccaagtctag gtaagaaaag acttcctcac ctggcacggc
     4081 atcttcaggc aagatttgga tgccatctgc gaattcctta ggcacaactg agtcagaaat
     4141 tcccaattca ccaagtgaac agatcattcc aagtgactcc aaaccacgga tttttccttt
     4201 tttgattttg tagttatcag cgatacgagc tcctggaaga gccaccatga ccttgatccc
     4261 agcacgcaca tttggggcac cacaaacgat ctgacgctct tcttcgccaa cgttaacctg
     4321 acaaacatgg aggtgagtct ctggcacatc ttcgcaagac aagacctcac cgacgacaat
     4381 ttttgagaga ccagcagctg gtgattcgac accctctacc tcgattcctg tagttgacat
     4441 tttttcagcc aactcttgtg atggcacatc aatgtccacc aattctttta accatttata
     4501 agatataagc ataatttagt tctccagaat gaccattgcc acactggttc ttttcctttc
     4561 ctatcatttc aatagaagaa tcctcttctt accttaattt ctttctcagt aaccaatccg
     4621 tatctacttt ttgaccaacc ataaaatgat gttggctaaa tttttcaaaa ccatatcgat
     4681 tataaaacgc ttgagctttt gtattatgct cccaaacacc tagccaagcc caagaaaaac
     4741 tattttttgt agcaagttca agtgcgaatt caaacagttg cttacctagt ccaaatcctt
     4801 ggaatttttg tagcacatag aggcgttgaa tttcaaaagc gtcctctaat tctctctcag
     4861 tttgagcact tccccagttg actttgagaa aaccagctat ctcctcctca tgcataatga
     4921 aataggtttc agagtcagga tttcccaact cagttgacaa agttttcaga ctataagcct
     4981 cttcaaagta ttcctgtaac tgctcttccg tattatcata cgcaaaggtt tcacgaaagg
     5041 cttgtttggc aattttagcc aacacctcaa catctgccat ttctactttt ctaatcatta
     5101 tttaaactgt tctgagaagc ggacatctcc ttggtagaat ccacggatat cgttgattcc
     5161 ataacggagc atagctacac gctcttgtcc aagaccaaag gcaaaaccag agtatacagt
     5221 cgcatcgata ccactcattt caaggacacg tgggtgaacc ataccggctc ccataatttc
     5281 gatccaacct gttttcttac atacgttaca gccttctcca ccacacttga agcaagaaac
     5341 atccacctca acagatggct ctgtgaatgg gaagtaagat ggacgcaaac gaatttgacg
     5401 ctcttcacca aacatttttt ggacaatcaa ctgaagcgtt ccttgaagat cagccataga
     5461 gatatttttc ccaactacca agccttcgat ttggtggaat tggtgactgt gggtcgcatc
     5521 gtccgtatcg cgacggaaga cacgccctgg cgagatcatc ttcaaaggac ctttagaaaa
     5581 atcatgggca tccatagcac gcgcctgaac tggagacgtg tgggtacgga gcaagatttc
     5641 ttcagtgata tagaaagtat cctgcatatc acgagctggg tggtcttttg gaaggttcat
     5701 acgttcaaag ttatagtagt cttgctccac ttcaaaacca tccacgactt gataacccat
     5761 accgatgaag atatcttcga tttcttcact ggtttgtgtc aaaacgtgac ggtgaccagt
     5821 cgcaactgga cgacctggaa gcgtcacatc gatactctcg ctagccagtt gagccgcgac
     5881 tttcttttct tccaagagct tagctgtttc ttcaaaagca gctgtcaaga catcacgagc
     5941 ttcattgacg tgtttcccga tgattggacg catctcagca gaaacatctt tcatcccttt
     6001 gaggatttca gtgagcgaac cctttttacc aaggacagag acacgcaaat cttgcatctc
     6061 tttttcattt ccagcagtaa tctgcttcaa gctagtcagc gtttcttcgc gaagcgcttt
     6121 taattgttct tcaatagttg acatatttcc tccatcagtc tctcgtagat aaaaagaaaa
     6181 ccacatgcca aaaactccac tcggagcgtt gacacgcggt accatccgtt ttcatctgac
     6241 aagtcagacc ttcatttcta aatccatgcg caagtgaatt cacccagctt tcatatagag
     6301 agcttgcagt cacggctctc ctccctgata tacttccctt gggctactag tctttcagat
     6361 ttctattcaa ttaccactta gtttatcaga tttttaccat tcttgcaaga cctatcttac
     6421 ttctgcttgt tagcttattc ttatctaact ttatagcgga ttgaaataag atga
//
LOCUS       DAVLSL010000105         6447 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLSL010000105 DAVLSL010000000
VERSION     DAVLSL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.107"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            110..310
                     /locus_tag="VXN30_001580"
     CDS             110..310
                     /locus_tag="VXN30_001580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1358182.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            345..1061
                     /gene="atpB"
                     /locus_tag="VXN30_001581"
     CDS             345..1061
                     /gene="atpB"
                     /locus_tag="VXN30_001581"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1358183.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            1075..1569
                     /gene="atpF"
                     /locus_tag="VXN30_001582"
     CDS             1075..1569
                     /gene="atpF"
                     /locus_tag="VXN30_001582"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1358184.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            1569..2105
                     /locus_tag="VXN30_001583"
     CDS             1569..2105
                     /locus_tag="VXN30_001583"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1358185.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            2120..3625
                     /locus_tag="VXN30_001584"
     CDS             2120..3625
                     /locus_tag="VXN30_001584"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1358186.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVHFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            3641..4519
                     /locus_tag="VXN30_001585"
     CDS             3641..4519
                     /locus_tag="VXN30_001585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1358187.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            4605..6011
                     /gene="atpD"
                     /locus_tag="VXN30_001586"
     CDS             4605..6011
                     /gene="atpD"
                     /locus_tag="VXN30_001586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1358188.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            6022..6441
                     /locus_tag="VXN30_001587"
     CDS             6022..6441
                     /locus_tag="VXN30_001587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1358189.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDNKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
BASE COUNT         1917 a         1207 c         1425 g         1898 t
ORIGIN      
        1 aaatggaaat aatttttatt aaacgcttcc aatagcggtt aaaagttgac aaatttaaat
       61 tttaggacta cactaagtaa gtaaatttta ttttaaagga gaatttgtta tgaatttaac
      121 atttttaggc ttatgtattg cctgtatggg cgtatctgtc ggtgaaggtt tattgatgaa
      181 tggactgttt aaatcagtag cacgccaacc agatatgctt tctgagtttc gtagtttgat
      241 gtttttaggt gttgccttta ttgaaggaac tttctttgta actcttgtct tctcatttat
      301 tatcaaataa atacatggaa cgagaagaaa agggaggatt ttagatggaa gaaagtatta
      361 atccaatcat ctctattggt cctgttatct tcaatctgac tatgttagcc atgactttgt
      421 tgattgtggg agttattttt gtctttattt attgggcaag ccgcaatatg accttgaaac
      481 ccaaaggaaa gcaaaatgta cttgagtatg tctatgactt tgttattgga tttacagaac
      541 ctaacattgg ttcgcgctac atgaaagatt actcactctt tttcctttgt ttattccttt
      601 tcatggtgat tgccaataac cttggcttaa tgacaaagat tcaaacgatc gatgggacta
      661 actggtggag ttcgccaacc gctaatttac agtatgactt aaccttatct tttcttgtca
      721 ttttgttgac acatatagaa agcgttcgtc gtcgtggatt taaaaaaagt ataaaatctt
      781 ttatgagtcc tgtttttgtc ataccgatga atatcttgga agaatttaca aacttcttat
      841 ctttggcttt gcggattttt gggaatatct ttgcaggaga ggtcatgacg agtttgttac
      901 ttcttctttc ccaccaagct atttattggt atccagtagc ctttggagct aatttggctt
      961 ggactgcatt ttctgtcttt atttcctgca tccaagctta tgtttttact cttttgacat
     1021 ctgtgtattt agggaataag attaatattg aagaggaata gaaaggagta actgatgcac
     1081 gtaacagtag gtgaattaat tggtaatttt gttttaatca ctggctcttt tattcttttg
     1141 ctagtcttga ttaaaaaatt tgcatggtct aatattacag gcattttcga agaaagagct
     1201 gaaaaaattg cttcagatat tgacagagct gaagaagccc gtcaaaaagc agaagtattg
     1261 gctcaaaaac gcgaagatga attggctggt agccgtaaag aagctaagac aatcattgaa
     1321 aatgcaaagg aaacagctga gcaaagtaag gctaatatct tagcagatgc taaactagaa
     1381 gcaggacact taaaagaaaa agccaatcaa gaaattgctc aaaataaagt agaagcttta
     1441 cagagtgtta agggtgaggt cgcagatttg accatcagct tagctggtaa aatcatctca
     1501 caaaaccttg acagtcatgc ccataaagca ctcattgatc agtatatcga tcagctagga
     1561 gaagcttaat ggacaagaaa acagtaaagg taattgaaaa atacagcatg ccttttgtcc
     1621 aattggtact tgaaaaagga gaagaagacc gtatcttttc agacttgact caaatcaagc
     1681 aagttgttga aaaaacaggt ctgccttctt ttttaaaaca agtggcagta gacgagtcgg
     1741 ataaggaaaa aacaattgct tttttccaag attctgtgtc acctttatta caaaacttta
     1801 tccaggttct ggcctacaat cacagagcaa atctttttta tgatgtgctt gtagattgct
     1861 tgaaccgact tgaaaaagaa acaaatcgat ttgaagtgac gattacgtct gctcatcctc
     1921 taactgatga acagaagact cgtttgctcc ctttgattga gaaaaaaatg tctctgaaag
     1981 taaggagtgt aaaagaacaa atcgatgaaa gtctcattgg tggttttgtc atttttgcca
     2041 atcacaagac aattgatgtg agtattaaac aacaacttaa agttgttaaa gaaaatttga
     2101 aatagaaagt ggtgttcttt tggcaattaa cgcacaagaa atcagcgctt taattaagca
     2161 acaaattgaa aatttcaaac ccaattttga tgtgactgaa acaggtgttg taacctatat
     2221 cggggacggt atcgcgcgtg ctcacggcct tgaaaatgtc atgagtggag agttgttgaa
     2281 ttttgaaaac ggctcttatg gtatggctca aaacttggag tcaacagacg ttggtattat
     2341 catcctaggt gactttacag atatccgtga aggcgataca atccgccgta cagggaaaat
     2401 catggaagtc cctgtaggtg aaagtctgat tggtcgtgtt gtggatccgc ttggtcgtcc
     2461 agttgacggt cttggagaaa tccacactga taaaactcgt ccagtagaag caccagctcc
     2521 tggtgttatg caacgtaagt ctgtttcaga accattgcaa actggtttga aagctattga
     2581 cgcccttgta ccgattggtc gtggtcaacg tgagttgatt atcggtgacc gtcagacagg
     2641 gaaaacaacc attgcgattg atacaatctt gaaccaaaaa gatcaagata tgatctgtat
     2701 ctacgtcgcg attggacaaa aagaatcaac agttcgtacg caagtagaaa cacttcgtca
     2761 gtacggtgcc ttggactaca caatcgttgt gacagcctct gcttcacaac catctccatt
     2821 gctcttccta gctccttatg ctggggttgc tatggcggaa gaatttatgt atcaaggtaa
     2881 gcatgttttg attgtatacg atgatctatc aaaacaagcg gtagcttatc gtgaactgtc
     2941 gctcttgctt cgtcgtcctc caggtcgtga agccttccca ggggatgttt tctatctcca
     3001 cagccgtttg cttgagcgct cagctaaagt ttctgatgaa cttggtggtg gatcaattac
     3061 agccctacca tttatcgaga cacaagcagg agatatctca gcctatatcg caaccaacgt
     3121 gatttctatc actgatggac aaatcttcct tggcgatggc ctcttcaatg caggtattcg
     3181 tccagccatc gatgcgggtt catctgtatc tcgtgtaggt ggttctgcac aaatcaaagc
     3241 catgaagaag gttgctggta cacttcgtat cgaccttgct tcataccgtg agttggaagc
     3301 ctttactaag tttggttctg acttggacgc agcaacacag gctaagttga accgtggacg
     3361 tcgtaccgtt gaggtcttga aacaacctgt tcacaaacca ttacctgttg agaaacaagt
     3421 aaccattctt tatgctttga cacatggttt cttggatact gttccagtag atgatattgt
     3481 tcatttcgag gaagagttcc ataccttctt tgatgctcaa catccagaga ttttggaaac
     3541 cattcgtgat acaaaagact tgccagaaga agcagtcttg gatgctgcga ttacagagtt
     3601 tctcaatcaa tctagcttcc aataagaata gaggtgtcag atggcagtat ctctaaatga
     3661 tattaaaaca aaaatcgcct caacaaaaaa tacgagtcaa atcactaatg ccatgcaaat
     3721 ggtatcggct gctaagctag gtcgttctga agaagctgct cgcaacttcc aagtttacgc
     3781 tcagaaagtg cgtaaacttt tgacagatat ccttcatggt aatggagctg gtgcttcaac
     3841 taatccgatg ttgattagcc gttctgtgaa gaagacaggc tatatcgtta tcacttcaga
     3901 ccgcggtttg gttggaggtt ataattcctc tattttgaaa gctgttatgg agttgaaaga
     3961 agaataccac ccagacggta aaggttttga aatgatctgt atcggtggga tgggagctga
     4021 tttctttaag gctcgcggta ttcaaccact ttatgaatta cgtggcttgg cagaccaacc
     4081 tagctttgat caagttcgta agattatttc aaaaactgtt gaaatgtacc aaaatgaact
     4141 ctttgatgag ctttatgttt gctacaacca ccatgtcaat acgctaacca gtcaaatgcg
     4201 tgtggaacaa atgcttccga ttgttgactt ggatccaaat gaagcggatg aagagtacag
     4261 cttgactttt gaattggaaa ccagccgaga agaaattctg gagcagttgt tgcctcagtt
     4321 tgcagaaagt atgatttacg gtgccattat cgatgccaag acagctgaga atgctgcggg
     4381 tatgacagcc atgcaaacag cgacagataa tgctaagaaa gtcatcaatg atttgacaat
     4441 tcagtataac cgtgccagac aggcggcgat tacacaagaa attacagaaa tcgtagcagg
     4501 tgctagtgcc ttagaatagg ctctagtcca gctcgtatga aaatgaactt aggacctagt
     4561 tgagctagga accgacagta tcttatatag aataggagaa ggagatgagt tcaggtaaaa
     4621 ttgctcaggt tatcggtccc gttgtagacg ttttgtttgc agcaggggaa aaacttcctg
     4681 agattaacaa tgcacttgtc gtctacaaaa atgacgaaag aaaaacaaaa atcgtccttg
     4741 aagtagcctt ggagttagga gatggtatgg ttcgtactat cgccatggaa tcaacagatg
     4801 ggttgactcg tggaatggaa gtattggaca caggtcgtcc aatctctgta ccagtaggta
     4861 aagaaacttt gggacgtgtc ttcaacgttt tgggagatac cattgatttg gaagctcctt
     4921 ttacagaaga cgcagagcgt cagccaattc ataaaaaagc tccaactttt gatgagttgt
     4981 ctacctcttc tgaaatcctt gaaacaggga tcaaggttat tgaccttctt gccccttacc
     5041 ttaaaggtgg taaagttgga cttttcggtg gtgccggagt tggtaaaacc gtcttaatcc
     5101 aagaattgat tcacaacatt gcccaagagc acggtggtat ttcagtattt actggtgttg
     5161 gggaacgtac tcgtgagggg aatgaccttt actgggaaat gaaagaatca ggcgttatcg
     5221 agaaaacagc catggtcttt ggtcagatga atgagccacc aggagcacgt atgcgtgttg
     5281 cccttactgg tttgacaatc gctgaatact tccgtgatgt ggaaggccaa gacgtgcttc
     5341 tctttatcga taatatcttc cgtttcactc aggctggttc agaagtatct gcccttttgg
     5401 gtcgtatgcc atcagccgtt ggttaccaac caacacttgc tacggaaatg ggtcaattgc
     5461 aagaacgtat cacatcaacc aagaagggtt ctgtaacctc tatccaggct atctatgtgc
     5521 cagcggatga ctatactgac ccagcgccag caacagcctt cgctcacttg gattcaacaa
     5581 caaacttgga acgtaaattg gtacaattgg gtatctaccc agccgttgac ccacttgctt
     5641 caagctcacg tgccttggca cctgaaatcg ttggagaaga gcactatgca gttgctgctg
     5701 aagtaaaacg tgtccttcaa cgttaccatg aattgcaaga tatcattgct atccttggta
     5761 tggatgagct ttctgatgaa gaaaagacct tggttgctcg cgcccgtcgt atccagttct
     5821 tcttgtcaca aaacttcaac gttgcggaac aatttactgg tcagccaggt tcttatgttc
     5881 cagttgctga aactgtacgt ggctttaagg aaatccttga tggtaaatac gaccacttgc
     5941 cagaagatgc cttccgtggt gtaggttcta tcgaagatgt gattgcaaaa gctgaaaaaa
     6001 tgggatttta agaggtgatc tatggctcag ttaactgtcc agatcgtgac accagatggt
     6061 ctcgtctatg atcaccatgc cagctatgta tcggttcgaa ctctggatgg tgagatgggg
     6121 atcttgccac gacatgaaaa tatgattgca gttttagcag ttgatgaagt aaaggtaaaa
     6181 cgtatcgata ataaagatca cgtgaactgg attgcagtaa acggtggcgt tattgaaatt
     6241 gccaatgata tgatcacaat cgtcgctgac tctgcagaac gtgctcgtga tatcgatatc
     6301 agtcgtgcag aacgtgccaa acttcgtgca gaacgtgcaa ttgaagaagc acaagacaaa
     6361 catttgattg accaagaacg tcgtgctaag attgctttgc aacgtgctat taaccgtatt
     6421 aatgtcggaa atagactata agaaaaa
//
LOCUS       DAVLSL010000106         6391 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLSL010000106 DAVLSL010000000
VERSION     DAVLSL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.108"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            441..1619
                     /locus_tag="VXN30_001588"
     CDS             441..1619
                     /locus_tag="VXN30_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1358190.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            1933..2064
                     /locus_tag="VXN30_001589"
     CDS             1933..2064
                     /locus_tag="VXN30_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1358191.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2085..3635
                     /gene="dltA"
                     /locus_tag="VXN30_001590"
     CDS             2085..3635
                     /gene="dltA"
                     /locus_tag="VXN30_001590"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1358192.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWISTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3632..4876
                     /gene="dltB"
                     /locus_tag="VXN30_001591"
     CDS             3632..4876
                     /gene="dltB"
                     /locus_tag="VXN30_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1358193.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4890..5129
                     /gene="dltC"
                     /locus_tag="VXN30_001592"
     CDS             4890..5129
                     /gene="dltC"
                     /locus_tag="VXN30_001592"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1358194.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5122..6390
                     /gene="dltD"
                     /locus_tag="VXN30_001593"
     CDS             5122..6390
                     /gene="dltD"
                     /locus_tag="VXN30_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1358195.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
BASE COUNT         1799 a         1162 c         1331 g         2099 t
ORIGIN      
        1 acggcacgta cggtggtgtg agaggggcta gagattatcc cctactcgat taactcccct
       61 gaaatttatt ttaattatgc aaatttcacg tatttttgat gctgagacga cgatcctggg
      121 aacttttcag atattttttt gactatctaa atctatcatt agaaaagctt agagcgccaa
      181 aggatttgag cgtttttctg atttttaaga ctttttccag tctctttttc gattgaagat
      241 gtaattattc tactaactaa ctaacttctt agtactagcc aacaacgata atcataattc
      301 ctcctaaaat taggaataat aaaggcaata gtttttgttt tttcatgtaa aaaacctcac
      361 ttttgttttc tgctatttta tgctaaaata ttaaaaatca aatttaattc caaagtttgt
      421 aactaaaggg ggagcgctac atgtctaatt catttgtcaa gttgttagtc tctcaattat
      481 ttgcaaattt agcagatatt ttctttagag taacaatcat tgctaacata tacattattt
      541 caaaatcagt aattgccaca tcactagttc ctatcttaat aggaatatcc tcttttgttg
      601 cgagtctttt agttccgttg gttactaaaa ggttagcgct aaatagggtt ttatctttat
      661 ctcaatttgg aaagactata ttattggcga tactggtagg aatgtttacc gtaatgcaat
      721 ccgtagcgcc tttggtgacc tatctatttg ttgttgcaat ttccatacta gatggttttg
      781 cagcacccgt ttcctatgct attgtgccac gctatgcgac cgatttgggt aaggctaatt
      841 cagccttatc aatgactggt gaagctgttc aattgatagg ttggggatta ggtggactct
      901 tgtttgcaac aattggtctg ttacctacca cgtttatcat tttagtcttg tatatcattt
      961 ctagctttct gatgttattt cttcctaacg ctgaagtgga ggtgttagag tcagaaacta
     1021 atcttgaaat tttgctcaaa ggttggaagt tagttgctag aaatcctaga ttaagacttt
     1081 ttgtatcagc aaatttattg gaaatttttt caaatacgat ttgggtttct tccattatac
     1141 ttgtttttgt aacggagtta ttaaataaaa cggaaagtta ctggggatat tctaatacag
     1201 catactctat tggtattata attagtggct taattgcttt taggctatct gaaaagttcc
     1261 ttgctgctaa atgggaaagt attctttttc ctcttgtagc tatggcaata gtgacactga
     1321 ctattctata ttttccaaac gcacagatgt ttttattatt ttcagcttta gttggtatgt
     1381 tatcgcaact aaaagaagtt cctgaaagtg tatttcttca ggaaacagta gaggaaaatc
     1441 atttagttaa tgtctattcc gttcttgaag tgatttctac attagcattt tcagtatttg
     1501 ttttgctaat gagctatatt actgagagtt ttggtattag catcagtttt tggctatcag
     1561 ccatttgtct gatgatagaa gctattttaa tttatatcag acgagattat tttaaatgag
     1621 agagtggtta aaaagtcttt aaaaatcaga aaagcgcata gtatcaggtg ttgaaaaaac
     1681 ttgatattgt gtgttttatt atgggaagat ttacttcatt gtctcctgaa atggagtttt
     1741 gaagcaggat cactctattt gaaaatatac atagaaaaac tgtataatcc ttaaaataag
     1801 ctgaaatcta ttggaaacat acggtaaaca attgaaaaga tactatataa agtggtacaa
     1861 tggtagaaaa ataagctagt aagaatcact cttattttag cgaaaaatta cagaaatgaa
     1921 tggtttttct tgatgaaaca gagaaaagaa ttgtacctct ttcttggtcg gacagccttg
     1981 tattttctta tctttctagg gctgctttac ttctttagct atcttggtca gggtcaagga
     2041 agctttatct ataatgaatt ttaacttgaa ggagaatccc tattgtgtca aataaaccaa
     2101 tagcagatat gattgaaacc attgagcatt ttgctcagac acagcctagc tatcctgtct
     2161 ataatgtttt ggggcaggaa cacacttatg gcgatttaaa ggctgattcg gatagtttgg
     2221 ctgcagtcat tgaccaacta ggcttgcctg agaagtctcc tgtggttgtt tttggtggcc
     2281 aagaatatga aatgttggca acctttgtag cgctgactaa gtcaggtcat gcctacattc
     2341 caattgatag ccattcggcc ttggagcgag tttcagctat tttagaagta gcagagccaa
     2401 gcttgattat tgccatttca gcctttccct tggagcaggt ttctacacca atgataaatc
     2461 tagctcaggt tcaagaagcc tttgcccaag ggaacaacta tgaaatcacg catccagtca
     2521 agggagatga taattactac attatcttta cttctggtac gactggtaag cctaagggag
     2581 tgcagatttc acatgataat ctcctcagct ttacaaactg gatgattacg gataaggaat
     2641 ttgcgacacc gagtcgtccg caaatgctgg cacagccacc ttattctttt gacttgtctg
     2701 tcatgtattg ggcaccgacc ttggcacttg gtggtacgct tttcactctt ccttcagtca
     2761 tcactcagga cttcaagcaa ctctttgcgg ctatcttttc attgccaatc gctatctgga
     2821 tatcaacacc atcctttgca gatatggcca tgttgtctga atacttcaac agtgagaaaa
     2881 tgcctggaat cacgcatttc tactttgatg gtgaagaatt gacggtcaaa acagctcaaa
     2941 aactgcgcga gcgtttccca aatgcccgta tcatcaatgc ttacggccca acagaagcga
     3001 cagtagctct gtcagcagtt gccgtgacag acgagatgtt agcgactctc aaacgcctac
     3061 caatcggcta taccaaggct gattctccaa cctttatcat tgacgaggaa ggaaataaac
     3121 tgccaaatgg cgaacaggga gaaatcattg tttctgggcc agctgtttca aaaggttata
     3181 tgaacaatcc tgaaaaaaca gcagaagcct tctttgagtt tgaaggtctg ccagcctatc
     3241 acacaggcga tgtgggaacc atgacagatg agggcttgct gctctacggc ggacgcatgg
     3301 acttccagat taagtttaac ggttaccgca ttgagttaga agatgtctct caaaacctca
     3361 acaagtctcg ctttatcgaa tctgctgtcg cagtaccgcg ctataacaag gaccacaagg
     3421 tacaaaatct attggcttat gtcatcttaa aagacggtgt tcgtgagcag tttgagcgag
     3481 atatcgatat taccaaggcc atcaaggaag acctgacaga catcatgatg tcctacatga
     3541 tgccatctaa attcctttac cgagacagtt tgccactgac tccaaatgga aagattgaca
     3601 tcaaaggatt gattaacgag gtgaataaga gatgatggag ttttttcaac agcttcctca
     3661 tttagagcca tatggcaatc ctcagtattt tgtttatgtg attgctgcaa ccttgcccat
     3721 ctttataggc ctctttttca agaaacgctt tgcctggtat gaagtgttgg taagtctctt
     3781 ctttattgtc accatgttgg tgggtggaaa gaccaatcaa ctagctgcct tgggtattta
     3841 cctttgctgg gaaatattgc tcctgctttt ctacaagcat tatcgaaaaa gcaaggatgg
     3901 caagtgggtc ttctacttag ttagttttct gtccctactt ccgattatct ttgtcaaggt
     3961 gcaaccagct atcaatggaa cgcagtcttt gcttgggttc ttgggaattt cttaccttac
     4021 ctttcgttcg gttggaattg tcatcgagct gagagatgga gtgattaagg attttaccct
     4081 ctgggaattc ctccgtttcc ttctcttcat gccaactttc tcgagtggtc caatcgatcg
     4141 ctttaagcga tttaatgaaa attatcaggc tattcctgag cgagatgagt tgatggatat
     4201 gctggatgaa tctgtccgct atatcatgtg gggctttttg tataagttta tcctagctca
     4261 tgttttagga gagaccttac tacctcctct gaagaattta gccttgcagt caggtggctt
     4321 ctttaatctc tatgccttgg cagttatgta tacttttggt ctggaactct tctttgactt
     4381 tgcaggttat tctatgtttg ctttggccat ctcaaacttg atgggaatcc gtagccctat
     4441 caactttaac aagccctttt tatcaaggga tttaaaggag ttttggaatc gctggcatat
     4501 gagtctgtcc ttctggttcc gtgactttgt ctttatgcga atggtgatgg tgttaaccag
     4561 aaagaaagtc tttaaaaatc gtaatgtaac ctcaagcatg gcctacattg taaatatgct
     4621 gattatggga ttttggcatg gtgtgacctg gtactatatc gcctatggac tctttcatgg
     4681 actaggcttg gtcatcaatg atgcctgggt tcgcaagaaa aaaacgctca ataaggaacg
     4741 gaaaaaagca gggaaggctg ccctacctga gaatcgctgg attcagttgc ttggcatggt
     4801 tgtcactttc catgttgtca tgttgtcatt cttaatcttt tctggattct tgaataatct
     4861 atggtttaaa aaataaaagg aaataaaata tggatatcaa atcagaagtt attgaaatta
     4921 ttgatgagtt gtttatggaa gatgtttctg acatgatgga tgaagatctt tttgatgcag
     4981 gtgtcttgga tagtatggga acggttgagt tgattgtgga gattgagaac cgttttgaca
     5041 ttcgtgtccc tgtaacagag tttggtcgtg acgactggaa tacagctaat aaaatcatag
     5101 ctggtattgt ggagctacaa aatgcttaaa cgcttatgga tgatcttcgg accggtcttg
     5161 atcgctggtt tgttggtttt tctgctcatt ttcttttatc ctactgagat gcatcataat
     5221 ctaggagctg aaaagcgttc agcagtggct actactatcg atagttttaa ggagcgaagt
     5281 caaaaagtca gagcactatc tgatccaaat gtgcgttttg ttcccttctt tggctctagt
     5341 gaatggcttc gttttgacgg tgctcatcct gcggtattag ctgagaaata caatcgttcc
     5401 taccgtcctt atcttttagg acagggggga gctgcatcgc ttaaccaata ttttggaatg
     5461 caacagatgt taccacagct ggagaataaa caagttgtgt atgttatctc acctcagtgg
     5521 ttcagtaaaa atggctatga tccagcagcc ttccagcagt attttaatgg agaccagttg
     5581 actagttttc tgaaacatca atctggggat caggctagtc aatatgcagc gactcgctta
     5641 ctgcagcagt tcccaaacgt agctatgaag gacctggttc agaagttggc aagtaaagaa
     5701 gaattgtcga cagcagacaa tgaaatgatt gaattattgg ctcgttttaa tgaacgccaa
     5761 gcctcctttt ttggtcagtt ttcggttaga ggctatgtta actacgataa gcatgtagct
     5821 aagtatttaa aaatcttgcc agaccagttt tcttatcaag caatagaaga tgttgtcaaa
     5881 gcagatgctg aaaaaaatac ttccaataat gagatgggaa tggaaaatta tttctataat
     5941 gagcagatca agaaggattt gaagaaatta aaggattctc agaaaagctt tacctatctc
     6001 aagtcgccag agtataatga cttgcagttg gttttaacac agttttctaa atctaaggta
     6061 aacccgattt ttatcattcc acctgttaat aaaaaatgga tggactatgc tggtctacga
     6121 gaggatatgt accaacaaac ggtgcagaag attcgctacc agttagaaag tcaaggtttt
     6181 accaatatag cagatttttc taaggacggc ggggagcctt tctttatgaa ggacaccatt
     6241 caccttggtt ggttgggttg gttggctttt gacaaggcag ttgatccttt cctatccaat
     6301 cccacaccag ctccgactta ccatctgaat gagcgctttt tcagcaaaga ttgggcgact
     6361 tatgatggag atgtcaaaga atttcaatag a
//
LOCUS       DAVLSL010000107         6322 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLSL010000107 DAVLSL010000000
VERSION     DAVLSL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.109"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            25..1725
                     /locus_tag="VXN30_001594"
     CDS             25..1725
                     /locus_tag="VXN30_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1358196.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1718..2194
                     /gene="ilvN"
                     /locus_tag="VXN30_001595"
     CDS             1718..2194
                     /gene="ilvN"
                     /locus_tag="VXN30_001595"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1358197.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2260..3282
                     /gene="ilvC"
                     /locus_tag="VXN30_001596"
     CDS             2260..3282
                     /gene="ilvC"
                     /locus_tag="VXN30_001596"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1358198.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3389..3625
                     /locus_tag="VXN30_001597"
     CDS             3389..3625
                     /locus_tag="VXN30_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358199.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            3863..4219
                     /locus_tag="VXN30_001598"
     CDS             3863..4219
                     /locus_tag="VXN30_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1358200.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            4269..5519
                     /gene="ilvA"
                     /locus_tag="VXN30_001599"
     CDS             4269..5519
                     /gene="ilvA"
                     /locus_tag="VXN30_001599"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1358201.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1957 a         1048 c         1434 g         1883 t
ORIGIN      
        1 tttaagagga aagaaagggg aataatggag aaaatcagtt tagaatctcc taagacgggg
       61 tcggacctag ttttggaaac acttcgtgat ttaggagttg ataccatctt tggttatcct
      121 ggtggtgcgg ttttgccttt ttatgatgcg atatataatt ttaaaggcat tcgccacatt
      181 ctagggcgcc atgagcaagg ttgtttgcat gaagctgaag gctatgccaa atcaactgga
      241 aagttgggtg ttgccgtcgt cactagtgga ccaggagcaa caaatgccat tacagggatt
      301 gcggatgcta tgagcgatag cgttcccctt ttggtcttta caggtcaggt ggcgcgagca
      361 gggattggga aggatgcctt tcaggaggca gacatcgtag gaattaccat gccaatcact
      421 aagtacaatt accaagttcg tgagacagct gatattccgc gtatcattac ggaagctgtc
      481 catatcgcaa ctacaggccg tccagggcca gttgtaattg acctaccaaa agacatatct
      541 gctttagaaa cagacttcat ttattcacca gaagtgaatt taccaagtta tcagccgact
      601 cttgagccga atgatatgca aatcaagaaa atcttgaagc aattgtccaa ggctaaaaag
      661 ccagtcttgt tagctggtgg tggaattagt tatgctgagg ctgctacgga actaaatgaa
      721 tttgcagaac gctatcaaat tccagtggta accagtcttt tgggacaagg aacgattgca
      781 acgagtcacc cactctttct tggaatggga ggcatgcacg ggtcattcgc agcaaatatt
      841 gccatgacgg aagcggactt tatgattagt attggttctc gtttcgatga ccgtttgacg
      901 gggaatccta agactttcgc taagaatgct aaggttgccc acattgatat tgacccagct
      961 gagattggca agattatcag tgcagacatt cctgtagttg gagatgctaa gaaggccttg
     1021 caaatgttgc tagcagaacc aacagttcac aacaatactg aaaagtggat tgagaaagtc
     1081 actaaagaca agaatcgtgt tcgttcgtat gacaagaaag agcgtgtggt tcaaccgcaa
     1141 gcagttattg aacgaattgg tgaattgacg aatggagatg ccattgtggt aacagacgtt
     1201 ggtcaacacc aaatgtggac agctcagtat tatccctacc aaaatgaacg tcagttagtg
     1261 acttcaggtg gtttgggaac aatgggcttt ggaattccag cagcaatcgg tgctaaaatt
     1321 gctaacccag ataaggaagt agtcttgttt gttggggatg gtggtttcca aatgaccaac
     1381 caggagttgg ctattttgaa tatttacaag gtgccaatca aggtggttat gctgaacaat
     1441 cattcactcg gaatggttcg ccagtggcag gaatccttct atgaaggcag aacatcagag
     1501 tcggtctttg atacccttcc tgatttccaa ttgatggcgc aggcttatgg tattaaaaac
     1561 tataagtttg acaatcctga gaccttggct caagaccttg aagtcatcac tgaggatgtt
     1621 cctatgctaa ttgaggtaga tatttctcgt aaggaacagg tgttaccaat ggtaccggct
     1681 ggtaagagta atcatgagat gttgggggtg cagttccatg cgtagaatgt taacagcaaa
     1741 actacaaaat cgttcaggag tcctcaatcg ctttacaggt gtcctatctc gtcgtcaggt
     1801 taatattgaa agcatctctg ttggagcaac agaagatccg aatgtatcgc gtatcactat
     1861 tattattgat gttgcttctc atgatgaagt ggagcaaatc atcaaacagc tcaatcgtca
     1921 gattgatgtg attcgcattc gagatattac agacaagcct catttggagc gcgaggtgat
     1981 tttggttaag atgtcagcgc cagctgagaa gagagctgag attttagcga ttattcaacc
     2041 tttccgtgca acagtggtag acgtagcgcc aagctcgatt accattcaga tgacgggaaa
     2101 tgcagaaaag agcgaagccc tattgcgagt cattcgccca tacggtattc gcaatattgc
     2161 tcgaacgggt gcaactggat ttacccgcga ttaaaaatcc aacttaaatt tgttaaacca
     2221 gcttaaaagg caataaataa tagaaaagag agaaaagcta tgacagttca aatggaatat
     2281 gaaaaagatg ttaaagtagc agcacttgac ggtaaaaaaa tcgccgttat cggttatggt
     2341 tcacaagggc atgcgcatgc tcaaaacttg cgtgattcag gtcgtgacgt tattatcggt
     2401 gtacgtccag gtaaatcttt tgataaagca aaagaagatg gatttgatac ttacacagta
     2461 gcagaagcta ctaagttggc tgatgttatc atgatcttgg cgccagacga aattcaacaa
     2521 gaattgtacg aagcagaaat cgctccaaac ttggaagctg gaaacgcagt tggatttgcc
     2581 catggtttca acatccactt tgaatttatc aaagttcctg cggatgtaga tgtcttcatg
     2641 tgtgctccta aaggaccagg acacttggta cgtcgtactt acgaagaagg atttggtgtt
     2701 ccagctcttt atgcagtata ccaagatgca acaggaaatg ctaaaaacat tgctatggac
     2761 tggtgtaaag gtgttggagc ggctcgtgta ggtcttcttg aaacaactta caaagaagaa
     2821 actgaagaag atttgtttgg tgaacaagct gtactttgtg gtggtttgac tgcccttatc
     2881 gaagcaggtt tcgaagtctt gacagaagca ggttacgctc cagaattggc ttactttgaa
     2941 gttcttcacg aaatgaaatt gatcgttgac ttgatctacg aaggtggatt caagaaaatg
     3001 cgtcaatcta tttcaaacac tgctgaatac ggtgactatg tatcaggtcc acgtgtaatc
     3061 actgaacaag ttaaagaaaa tatgaaggct gtcttggcag acattcaaaa tggtaaattt
     3121 gcaaatgact ttgtaaatga ctataaagct ggacgtccaa aattgactgc ttaccgtgaa
     3181 caagcagcta accttgaaat tgaaaaagtt ggtgcagaat tgcgtaaagc aatgccattc
     3241 gttggtaaaa acgacgatga tgcattcaaa atctataact aattagaaat atatagcgct
     3301 ggagatgatt ttatgaaaaa gattatgaga aaaattgcat cgttattatt ggttctagtt
     3361 gtataatgta attacaccgt cggtaatagt gctagcagac caaaataaag cagattggtc
     3421 gtatgatgaa aatgctgtaa ttaacattta tgatgatgct aattttgaag atggtaggtt
     3481 gcatatgacc tttgaacaat tcttcaaatt ggcacaaata gctagagaag aaggtcttga
     3541 aattcattct ccgtttgaga gagctggtgc gactaaatct gctcgttata tagcgaaatg
     3601 gattttgaga aataaaaaat attaacaaat atagttggta aatcattagg acctaaatca
     3661 gctgttagat tcggagaagc tttatcctat attgaaggtc ctcttcgcag aataaatgag
     3721 acgatagatg gcggtttata tcaaatagag caaattattg catctggatt gaaagaatcg
     3781 ggtttaaatg actggactgc gaaaacttta gcttcagcta ttcgtgggat attagatgta
     3841 cttatttagg ggttgaaatc atatgaatat taccaatttg ttttctatca agacaggatg
     3901 tgatgaaact gataggcaac tgcaaaaact attttttcag ttggatttac aattgggaga
     3961 attgacagat caactaagaa aattagattc taattttgtt cctcgtagtc aatttgtaga
     4021 cacgttggat ttgaatgatg tagaatataa agaaatttta aactatttta tcttccatcg
     4081 taatgatagt gaagaaagtt tggtagaatg gttatatgat tggatttcca caaatcgtta
     4141 tgaacttcct aaagagtttt cgattcgtat ggctcataaa taccatgaaa gtgttactga
     4201 aattttcgga gatgaataac taaaaaacag tcattagtga ctgtttttta tagaaaaaga
     4261 ggttttatat gttaagttca aaagatataa tcaaggctca caaggtcttg aacggtgtgg
     4321 ttgtgaatac tccactggat tacgatcatt atttatcgga gaagtatggt gctaagattt
     4381 atttgaaaaa agaaaatgcc cagcgtgttc gctcctttaa aattcgtggt gcctattatg
     4441 ccatttccca gctcagcaag gaagaacgtg aacgtggggt agtttgcgct tctgcgggaa
     4501 atcatgcgca gggagtagcc tatacttgta atgaaatgaa aattcctgct actatcttta
     4561 tgcccattac tacgccacaa caaaagattg gtcaggttcg cttttttggt ggggattttg
     4621 taactattaa actagttgga gatacctttg atgcctcagc caaagcagct caagaattta
     4681 cagtctctga aaatcgtacc tttattgatc cttttgatga tgctcatgtt caagcaggtc
     4741 aaggaacagt tgcttatgag attttagaag aagctcgaaa agaatcgatt gattttgatg
     4801 ctgtcttggt tcctgttggt ggtggcggtc tcattgccgg ggtttctacc tatatcaagg
     4861 aaacaagtcc agagattgag gttatcggag tagaggcgaa tggagcgcgt tccatgaaag
     4921 ctgcctttga ggctggaggt ccagtcaaac tcaaggaaat tgataaattt gcggatggga
     4981 ttgctgtgca aaaggtaggt cagttgacct atgaagcaac tcgtcaacat attcaaactt
     5041 tggtaggtgt cgatgaggga ttgatttctg aaaccttgat tgacctttac tctaagcaag
     5101 ggatagtcgc agaacctgct ggagcagcta gtatcgcctc tttagaggtt ttagctgaat
     5161 atattaaggg gaaaaccatt tgttgtatca tttctggagg aaataatgat atcaaccgta
     5221 tgccagaaat ggaagagcgt gccttgattt atgatggtat caaacattat tttgtggtca
     5281 atttcccaca acgtccagga gctttgcgtg agtttgtaaa tgatatcctg gggccaaatg
     5341 atgatatcac acgttttgag tatatcaaac gagctagcaa gggaacaggc ccagtattga
     5401 ttgggatcgc tttagcagat aagcatgatt atgcaggttt gattcgtaga atggaaggtt
     5461 ttgatccagc ttatattaac ttaaatggta atgaaacgct ttataatatg cttgtctgag
     5521 gactaataaa aaaatatcat attatttgta caactgatgt atatatgcta taatgaagat
     5581 gaagaaaggg gacgattcct atggttttag aaaacttatc gtgcaattct tacttctgtt
     5641 cattatagca cttatgatgt ttggctcaga tttccatcct tcattttgat ttcctatcta
     5701 ttgacaagca tagtcacact gtctttaata ctcttcgaaa atctcttcaa accacgttag
     5761 ctctatctgc aacctcaaaa cagtgttttg agcaacttgc ggctagcttc ctagtttgct
     5821 ctttgatttt cattgagtat aaggtatgat ttgatttctt tttgttgaca aatataccat
     5881 attaaaaaga tatataagta attaactgag cttatctgtc ttgtcatctc tattaaggat
     5941 ggtttagata atcgggtgtc tgcttctagg ctagcacctc aatatccaaa ggagtgatga
     6001 atttgaagga tataagaaat acctatctct cagatgattt attgaggaag aaagatagga
     6061 gtttttgagc tagtgaaggc ttggatttct aaaggttaga actatcatct tcagttctta
     6121 aatcgaagaa ataagctatc ttacggaaat agagaagcat tttttaagaa cttgaataat
     6181 ttcgcacctt aagagggtaa taatacagta gttttattag caaatattta tggtgtagag
     6241 gctagcaaaa cctatatatt atcggattta aaaaggaagt aagaaaattc tgaactaatt
     6301 attttcgttg aatatatgtt tt
//
LOCUS       DAVLSL010000108         6319 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLSL010000108 DAVLSL010000000
VERSION     DAVLSL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.110"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            108..272
                     /locus_tag="VXN30_001600"
     CDS             108..272
                     /locus_tag="VXN30_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1358202.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            356..922
                     /gene="amaP"
                     /locus_tag="VXN30_001601"
     CDS             356..922
                     /gene="amaP"
                     /locus_tag="VXN30_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1358203.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            934..1104
                     /locus_tag="VXN30_001602"
     CDS             934..1104
                     /locus_tag="VXN30_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1358204.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1143..1751
                     /locus_tag="VXN30_001603"
     CDS             1143..1751
                     /locus_tag="VXN30_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1358205.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1782..1985
                     /locus_tag="VXN30_001604"
     CDS             1782..1985
                     /locus_tag="VXN30_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1358206.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2095..2373)
                     /locus_tag="VXN30_001605"
                     /pseudo
     CDS             complement(2095..2373)
                     /locus_tag="VXN30_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2571..2858
                     /locus_tag="VXN30_001606"
     CDS             2571..2858
                     /locus_tag="VXN30_001606"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1358207.1"
                     /translation="MIVSLKTKSRKAKDIAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3136..3906)
                     /locus_tag="VXN30_001607"
     CDS             complement(3136..3906)
                     /locus_tag="VXN30_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1358208.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3921..4769)
                     /locus_tag="VXN30_001608"
     CDS             complement(3921..4769)
                     /locus_tag="VXN30_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1358209.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIANTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHSAGAPWWLQVPNVCWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4766..6241)
                     /locus_tag="VXN30_001609"
     CDS             complement(4766..6241)
                     /locus_tag="VXN30_001609"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1358210.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALGGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEEIVPVELSKITPTLTKTGDSQS"
BASE COUNT         2182 a         1188 c         1037 g         1912 t
ORIGIN      
        1 attttttgat attaaaataa aaaaggaggg taggcagtgt tgtgatcaat tattgctgga
       61 ggtcttattg gtctcttggc aggtaaaatc actaaaaaag tagttctata ggaatcatcg
      121 caaatgtatt cgctggttta gtcggggcat atgcaggaca atctctttta ggtagttggg
      181 gtccatcaat cgctggaatg gctttgctcc catctattgt aggtgcagcg attgtgatta
      241 ctgtagtgtc attctttaca ggtagaaagt aaacttttcg ccagtaaagt tagcaaacta
      301 tttttaaatc aatgacggga aaaatagttt aaatgttaaa tcgaaaggat tgtatatgtc
      361 aaaagcaaag aaaatatgtt tcattatttt ctgtatttta atcttgacaa ttttccttcc
      421 tgttttgata gattatcatc aagttagtga tctaggtatt catctactta gctggagaca
      481 gaactccgta gttgaattct atcttgctag atatgtcttt tgggggacag tggttctatc
      541 aactttagtt ttattatcca ttttagttgt gatgttttat cctaaacgtt acttggaaat
      601 ccaacttgaa actaaaaacg atacattaaa attaaagaat tcggcaatcg aaggttttgt
      661 tagaagtttg gtgagtgatc atagattgat caagaaccca actgttcatg taaatttacg
      721 aaaaaataaa tgtttcgttc atgtagaagg taaaattctt ccttcagaca acatcgctga
      781 cagatgccaa ataattcaaa atgaaataac taatggattg aagcagtttt ttggtattga
      841 gcgtcaagta aaacttgaag ttgcagtaaa aaattaccaa ccaaaacctc aaaacaaaaa
      901 gactgttagt cgtgtgaagt aaggaagtaa aaaatggaat ggcttaaaca atatcgatat
      961 ccaattatcg ctggtctcat aggcgtattt ctggcttgtt tgattgtctc ctttggcttc
     1021 ttcaaaacaa tatttgtatt gattttagga gcactgggag ttgcagctgg attatatatc
     1081 gaaaaaaact atatagataa ataaaaaaat aaaaattact aatttaatta aaggagtttc
     1141 atatgtcaaa cgaaaaaaac acaaacacta acgtagaaaa gaaagatgct actgttgtag
     1201 ctcacgaaat caaaggggaa cttacttacg aagataaagt tatccaaaaa atcattggtc
     1261 tttcactaga aaacgtttca ggtcttttgg gaatcgatgg tggtttcttc tcaaatctta
     1321 aagaaaaaat cgttaacagc gatgacgtaa caagtggtgt taacgtagaa gttggtaaaa
     1381 cacaagttgc agttgactta aacgttattg ttgagtacca aaaaaatgtt ccagctttat
     1441 attcagaaat cagagaaatc gtatcttcag aagttgctaa aatgactgac ttggaaattg
     1501 ttgaaatcaa cgtaaacgtt gtcgacatca aaactaaaga acagcatgaa gcagactcag
     1561 taagccttca agatcgcgta tctgacgttg ctgaatcaac aggagaattc acttcagaac
     1621 aattcgaaaa agctaaatct ggtcttggat ctggtttctc aactgttcaa gaaaaagtta
     1681 gcgaaggtgt agaagctgtt aaaggtgcag caaatggtgt agtatctcac gaaaacactc
     1741 gtgtaaacta agataaaata aatataacag gagaaattat catgtcagta gaagaaaaat
     1801 taaatcaagc taaaggttct attaaagaag gtgttgggaa agccatcggt gatgaaaaaa
     1861 tggaaaaaga aggagcagct gaaaaagttg tttctaaagt aaaagaagtt gccgaagacg
     1921 ctaaagacgc tgtagaaggt gctgtagaag gtgttaaaaa catgttgagt ggcgacgata
     1981 aataaggtta aaagttactt tatctttttg gtaacattag tcaaaagagt ctgagtcaag
     2041 atgattctca gaaaacaaaa agctagagat tcccaattgc ggaactctag ctttttaatt
     2101 ttgcctcttt ctcttattat atttcagcag gttgttggcc atgagtatga atcccatgtc
     2161 aattctcact tgacgcttac ctctcagatg acatctctta taacccaaac aaacctttat
     2221 ctgcccaaag acagatttca tatcaatctt acgtttagcg aaaatttgtc tacccttgga
     2281 agataaaagt gcctgatatt ctttagtttt taaacactgg taacgttcat tcatatacag
     2341 tcccttttga ggggctgatt caggttcata atcgcagtca acattgattt caaggctgtt
     2401 tgctttctat ctcccctttt tcatgatata ttttcgtaca ggtgcttcaa tagtttctat
     2461 agtaaaatga accaaaaata gtacacgttg cggtataatg ttcttgtggc atattcatta
     2521 gattttcgta aaaagttctc tcctatttgt gagcgaacag gtatagtcaa ttgattgtca
     2581 gtttgaagac caagagtcgt aaggcaaagg atatagcaga atccatccaa gggtggctgg
     2641 cacaatttct agtgaacctc tttaaatcta ttacgtttga ctgtggaaaa gagttttcaa
     2701 aatggaaaga tatttccaat catcacgata gtgagagctt ttttgcgaat ctaggatgtc
     2761 caagacagcg gtgtctcaac gaacattcaa atagactttt aaggtgtcat gacttgccaa
     2821 agcagacaga tttcaacgaa gtcagtcaag aattttaggc tgggtaaaaa ctcaatttca
     2881 ggagaaaatg aaataaatat tcccacaata aaatgcatag tatcaaagtt tttccaacac
     2941 ctgatattat gcatttttta attttaaaga ctttttccca gcctcaagaa atcacatccc
     3001 acctaaatcc tttggatacc aaatacctca tcaggtattt tctgagttgg taagactgtc
     3061 tagcttaatt tgacaaatgg agggcgttaa atccagtttt tacaggaaat ctatttaaat
     3121 attttcacac tgaccttaca aaatatgaaa taaattatat aaagtcattc ccatgacaaa
     3181 gaataacatc cctttaaaac agtatcttac ccaattttca ggtaagactt ttgatacaac
     3241 cgtccccaat acccctccgc atatagaagc aagaaaaata actggaacag gtgctaaatg
     3301 atactgattt aaacctgtaa ccacaacaat tgtagccaaa tgagacagct gggaaaagaa
     3361 aataattgca agagaataca tcgttgcttc ttttatcgaa atagaaaaaa acaccatcaa
     3421 taaagataca ttgagcggac caccaccaat ccctaaaaaa ctagaaatca agcccaatag
     3481 aagaccacaa atcatataga atcctatctt atcaaattga taagtttttt taaaatctgt
     3541 taaaacaaat gatacaagta gcatcacaaa tagtaaaatc atttgaacaa tggatactag
     3601 ccgtactgat aattgagata acaaaacttg aaaaataaga tgacctagaa atcctcctaa
     3661 aacagaagaa aaggataccg taaaaatcaa tctccatttt atctctttac tttgtgaaaa
     3721 tcttttgatt gttgaaataa tcgccattat aaaaacagag aaactagaat aaaaagcaat
     3781 atcactgacc gaatggtacc caaaactatc cataagtggc ttaattataa ttccaccacc
     3841 catgcctgat atagaaccca atccactagc tattataatg attagaaaat aaataatctc
     3901 aaccactttt taagtccatc ctattctttc aaacatctaa aacctagatg gctactagtg
     3961 gaagtagaaa tacatccatt tctagcagct actcggtatc gattacaata agaacaatga
     4021 cataaaaagg aacctcctct gatagcatac tcctcatctt gtatcccata ttttggcaac
     4081 tcatagtcat cttcattaaa actagctaat aaggtatatc tcggattgcg acaccattcc
     4141 caaacattcc caatcatttg atatagacca aaatcattag gtaaaaattc atagacagga
     4201 gccgttccta taaatccatc taaggcagta ttttcataag gaaatcttcc ttgccaagta
     4261 ttggcatgat aatatccacc ttctaaaaga gtatctcccc aaggatattc agattgtctt
     4321 cctcctctag cagcatattc ccactgtgct tcagtcggta aagacattcc gctccaatta
     4381 cagaaagcca atgcatcttc taaagcaaca tgaactactg gatggtcttc taaacccact
     4441 agattactat tctcaccgta gggatgtttc cagcaaacgt tcggaacttg taaccaccac
     4501 ggagccccag cagaatgagg atatccctcc ctttctgcct ctggaacgaa caatataaaa
     4561 acaaaagacc actcttgtcg ctcagctagt gttttatagc ctgtttcttt cacaaattga
     4621 gcaaattctt gattcgtaac tggagtattc gcaatggaaa aatcctttac actaacagcg
     4681 acttgaggac cttcaaaatc atctaaaaag ccctcttcag aatttgttcc catcataaat
     4741 gtcccaccct tgattttttt cattttcatg attgactatc ccccgttttc gttagagttg
     4801 gcgttatttt agaaagttca acaggaacta tctcttcatc cacaacaaaa ccttcttctc
     4861 tatatctaag aaagtctact aattttttct tcatctgacg aacaataggt tggtattttt
     4921 cacttggata caaatcattc atttcatgcg gatctttttt catatcaaat aactgataat
     4981 gatttaaaac aggaaaccag ataaatttcc actggtctgt caaaatatat tgactagaat
     5041 cttttcctaa agcatgttca ccatgaaatt ctgttcgcca cccctcgtac tgtccaaata
     5101 ataaattctt aacactccgt ccatctagtt catctgtagt agttcctcct gccaaatcca
     5161 cgagtgaagg aaaaatatct tgaattttca ctaattgctt gattgttccc ctattaccag
     5221 ctatcaatcc agccggatcg taaataaacg aaggaatgtg gatacttccc tgataaggat
     5281 atccctttct aaacagataa tgttcaccaa gctgatctcc atgatccgaa acaaaccaga
     5341 taatcgtatc tttatcatga cgaaattctt ttagggcagt caaaaagcga ctaatttgat
     5401 ggtctatatg agtaatcaat ccaaaataag cagcaaccat tcttctttga tcatcttcct
     5461 ttaatttccc tcctaaagca taaatagacg gaatttgctt ttctaatact tcccaattcc
     5521 cgatatgcaa atcgagaaat tgaggcaagc gttccatata catatcaaaa tagtattttg
     5581 ggggatttaa tggagcatgt ggtttttcaa aagacatttt gagaaaaaaa ggaaccgttg
     5641 gatctcttct ttgtaaaaat gaaatggact cactcacaac ccagtttgtt ggatgtaact
     5701 tctcatcttt atcccaaggt ctagcttccc aagaattaca atccattccg tcatcaatca
     5761 aatcaacatc ataaccaact ttacccttta aaaaggctag atagtcacta gcataatcaa
     5821 attgacttcc ataagcctta tcatactttc tatcaacatg tagataccca tcgtgaagta
     5881 agacatgatc aaacccaagt ctctgtctgg aaggaaaaac atgcatctta ccaatacact
     5941 ctgtttgata acccatatct ttaaagactt ttggaagagt attagtaaaa ttccaaggca
     6001 cctcatcttg ataaccaaca cgtccacttt tatcttggtc taagccagtc aataaagctg
     6061 ccctagcagg aacgcaagaa ggaactggag aataggcatt ttcaaaatta taacctacac
     6121 tagccatcat atctaatgtt ggagtgctaa ctagcttatc cttactattc aaggataagg
     6181 cgtctgctct catttgatct acaacaatca aaataatatt tggctgtttt gtctgaacca
     6241 taaaatctcc tttctaatat ggcaaaagag gcacaagaag atatctacct ttactgcacc
     6301 cctttctata tcaatctct
//
LOCUS       DAVLSL010000109         6114 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLSL010000109 DAVLSL010000000
VERSION     DAVLSL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.111"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            162..1433
                     /locus_tag="VXN30_001610"
     CDS             162..1433
                     /locus_tag="VXN30_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1358211.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            1544..2836
                     /locus_tag="VXN30_001611"
     CDS             1544..2836
                     /locus_tag="VXN30_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1358212.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            2838..3680
                     /locus_tag="VXN30_001612"
     CDS             2838..3680
                     /locus_tag="VXN30_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1358213.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            3933..4733
                     /locus_tag="VXN30_001613"
     CDS             3933..4733
                     /locus_tag="VXN30_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1358214.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            4743..5729
                     /locus_tag="VXN30_001614"
     CDS             4743..5729
                     /locus_tag="VXN30_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1358215.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            5871..>6114
                     /locus_tag="VXN30_001615"
     CDS             5871..>6114
                     /locus_tag="VXN30_001615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358216.1"
                     /translation="MKIKVQTRKLAAGCSKHGFEVADRTDEVSSKHGFEVVDETDEVS
                     SKHGFEVADRTDEVSSKHGFEVADRTDEVSSKHGFEV"
BASE COUNT         1793 a         1355 c         1113 g         1853 t
ORIGIN      
        1 aaaatgcaaa attatgctat caaatcaagc ttaaaaattt tttaaaattt ttacaaaaaa
       61 tacttgcaac cgttttctat ttgtgctata ctaagctcat aaaggaaaac gtttgcgttt
      121 ccttatcaat aaaacttgct attctttggg aggaatacac tatgtcatct aaatttatga
      181 agagcactgc ggtgcttgga actgttacac ttgctagctt gcttttggta gcttgcggaa
      241 gcaaaactgc tgataagcct gctgattctg gttcatctga agtcaaagaa ctcactgtat
      301 atgtagacga gggatataag agctatattg aagaggttgc taaagcttat gaaaaagaag
      361 ctggagtaaa agtcactctt aaaactggtg atgctctagg aggtcttgat aaactttctc
      421 ttgacaacca atctggtaat gtccctgatg ttatgatggc tccatacgac cgtgtaggta
      481 gccttggttc tgacggacaa ctttcagaag tgaaattgag cgatggtgct aaaacagacg
      541 acacaactaa atctcttgta acagctgcta atggtaaagt ttacggtgct cctgccgtta
      601 tcgagtcact tgttatgtac tacaacaaag acttggtgaa agatgctcca aaaacatttg
      661 ctgacttgga aaaccttgct aaagatagca aatacgcatt cgctggtgaa gatggtaaaa
      721 ctactgcctt cctagctgac tggacaaact tctactatac atatggactt cttgccggta
      781 acggtgctta cgtctttggc caaaacggta aagacgctaa agacatcggt cttgcaaacg
      841 acggttctat cgcaggtatc aactacgcta aatcttggta cgaaaaatgg cctaaaggta
      901 tgcaagatac agaaggtgct ggaaacttaa tccaaactca attccaagaa ggtaaaacag
      961 ctgctatcat cgacggacct tggaaagctc aagcctttaa agatgctaaa gtaaactacg
     1021 gagttgcaac tatcccaact cttccaaatg gaaaagaata tgctgcattc ggtggtggta
     1081 aagcttgggt cattcctcaa gccgttaaga accttgaagc ttctcaaaaa tttgtagact
     1141 tccttgttgc aactgaacaa caaaaagtat tatatgataa gactaacgaa atcccagcta
     1201 atactgaggc tcgttcatac gctgaaggta aaaacgatga gttgacaaca gctgttatca
     1261 aacagttcaa gaacactcaa ccactgccaa acatctctca aatgtctgca gtttgggatc
     1321 cagcgaaaaa tatgctcttt gatgctgtaa gtggtcaaaa agatgctaaa acagctgcta
     1381 acgatgctgt aacattgatc aaagaaacaa tcaaacaaaa atttggtgaa taaaaaattt
     1441 gttcaagggg ggtggaaatc aaatccccct ttgaatttat caatagagac acaaataatt
     1501 tagctttctt ataaaaaagt agtatcctat gaaaggagtt aatatggaaa agcaacaacc
     1561 tagtaaagca gccctgctgt ctatcattcc tgggttagga cagatttaca ataaacaaaa
     1621 agccaaaggt tttatcttcc ttggtgtaac catcgtattt gtcctttact tcctagcact
     1681 tgcaacccct gaattgagca acctcatcac tcttggtgac aaaccaggtc gtgataattc
     1741 cctctttatg ctgattcgtg gtgccttcca tctaatcttt gtaatcgttt atgtactctt
     1801 ttatttctca aatatcaaag atgcacatac gattgcaaaa cgcattaaca atggaattcc
     1861 agttccacgc acactcaaag acatgatcaa agggatttat gaaaatggct tcccttacct
     1921 cttgatcatt ccatcttatg ttgccatgac cttcgcgatt atcttcccag ttatcgtaac
     1981 cttgatgatc gcctttacca actacgactt ccaacacttg ccaccaaaca agttgttgga
     2041 ctgggttggt ttgaccaact ttacaaacat ttggagcttg agtaccttcc gttctgcctt
     2101 tggttctgtt ctttcttgga ctatcatttg ggctttggca gcttctactt tacaaatcgt
     2161 aattggtatc ttcacagcta tcattgccaa ccaaccattt atcaaaggaa aacgtatctt
     2221 tggtgttatt ttccttcttc cttgggctgt cccagccttc atcactatct tgacattctc
     2281 aaatatgttt aacgatagtg tcggtgctat caacactcaa gtattgccaa tcttggctaa
     2341 attccttcct ttccttgatg gagctcttat tccttggaaa acagacccaa cttggactaa
     2401 gattgccttg attatgatgc aaggttggct cggattccca tacatctacg ttttgacttt
     2461 gggtatcttg caatctattc ctaacgacct ttacgaagca gcttatattg acggtgccaa
     2521 cgcttggcaa aaattccgca acatcacttt cccaatgatt ttggctgttg cggcacctac
     2581 tttgattagc caatacacct tcaactttaa caacttctct atcatgtacc tcttcaatgg
     2641 tggaggacct ggtagtgtcg gaggtggagc tggttcaacc gatatcttga tctcatggat
     2701 ctaccgtttg acaacaggta catctcctca atactcaatg gcggcagctg ttaccttgat
     2761 tatctctatc attgtcatct caatctctat gatcgcattc aagaaactac acgcatttga
     2821 tatggaggac gtctaagatg aataactcaa ttaaactcaa acgtagactg actcaaagcc
     2881 ttacttacct ttacctgatt ggtctatcaa ttgtaattat ctatccactg ttgattacca
     2941 ttatgtcagc ctttaaagca ggtaacgtct cagcctttaa actagatact aatatcgacc
     3001 tcaattttga taactttaaa ggcctcttca ctgaaacctt gtacggtact tggtacctca
     3061 acactttgat tatcgcctta attaccatgg ctgttcaaac aagtatcatc gtacttgctg
     3121 gttatgctta cagccgttac aacttcttgg ctcgtaaaca aagtttggtc tttttcttga
     3181 tcatccaaat ggtgccaact atggccgctt tgacagcctt cttcgttatg gcgcttatgt
     3241 tgaacgccct taaccacaac tggttcctca tcttcctcta cgttggtggt ggtatcccga
     3301 tgaatgcttg gctcatgaaa ggctacttcg atacagtgcc aatgtcttta gacgaatctg
     3361 caaaactaga cggtgcagga cacttccgcc gcttctggca aattgttcta ccacttgttc
     3421 gcccaatggt tgccgtacaa gctctctggg ccttcatggg acctttcggg gactacatcc
     3481 tctctagttt cttgcttcgt gagaaagaat actttactgt tgccgtaggt ctccaaacct
     3541 tcgttaacaa tgcgaagaac ttgaagattg cctacttctc agcaggtgct atcctcatcg
     3601 cccttccaat ctgtattctc ttcttcttcc tacaaaagaa ctttgtttca ggacttacaa
     3661 gtggtggcga caagggataa tttatccccg ccaccctttt tcattttata ctcttcgaaa
     3721 atctcttcaa accacgtcag ctttatctcc aacctcaaag ttgtgctttg agcaacctgt
     3781 ggctagtttg cactttgatt ttcattgatt attagcaatt gtcactgtaa ataatatcct
     3841 tgtagcaagc aatttttctc ctagacttga aataaagcgc atttctctat ataataatac
     3901 tcatatagaa aacacctttt agaaagatac ctatgcttcc atatccattt tcctattttt
     3961 caagtatttg ggggtttcgt aagcccctgt ccaaacgttt cgagctcaac tggtttcaac
     4021 ttctctttac cagtatcttc cttatcagct tgtctatggt acccattgct atccaaaaca
     4081 gctcccagga gacctatccg ctagaaactt ttatcgataa tgtctatgaa cctctgacaa
     4141 atgaggttgt ccaggatctc tctgaacatg ctacaattgt cgatggcaca ttaacttata
     4201 ctggaacagc tagtcaagcc ccttctgttg tgattggtcc aagtcaaatc aaggaattac
     4261 ctaaggactt gcaactgcat ttcgatacaa atgagctagt catcagcaag gaaagcaagg
     4321 aactgacccg catctcttac cgagccattc agactgagag tttcaaaagc aaagacagct
     4381 tgacccaagc aatttctaaa gactggtacc aacaaaatcg tgtctatatc agcctcttcc
     4441 tagttctcgg tgcgagcttc ctctttggtt tgaatttctt tatcgtctct ctaggagcta
     4501 gtcttctcct ttatatcact aagaaatcac gcctcttttc atttagaacc tttaaagagt
     4561 gctatcactt tatcttgaac tgtttaggat tgccgactct gattacactt attttgggat
     4621 tatttggcca aaatatgaca accctgatta ctgtacaaaa tattcttttt gttctgtatc
     4681 tggtcactat cttttataaa acacatttcc gtgatccaaa ttaccataaa taggagattt
     4741 ttatgcccgt tacgattaaa gacgtggcca aggctgctgg tgtttcgcct tcaaccgtaa
     4801 cccgtgttat tcaaaataaa tcaaccatta gcgacgaaac aaaaaaacgc gttcgcaaag
     4861 ctatgaagga actcaactac cacccaaacc tcaacgctcg tagcttggta agcagctata
     4921 ctcaggttat cggattagtt cttcctgatg actcagacgc cttctaccag aatcctttct
     4981 ttccatcggt tctacgtggc atctctcaag tcgcatctga aaaccactat gccattcaga
     5041 tagcaacagg gaaagatgag aaggagcgtc tcaacgctat ttcacaaatg gtctacggca
     5101 agcgtgtaga tgggctaatc tttctctatg cccaagaaga agaccctctc gtaaaactcg
     5161 tcgcagaaga acagttcccc ttccttatct taggtaaatc tctatctcct ttcatcccac
     5221 ttgtcgacaa cgacaatgtt caagctggtt ttgatgcgac tgaatatttc atcaaaaaag
     5281 gctgcaaacg cattgccttt atcggaggaa gtaaaaagct cttcgtgacc aaagaccgtt
     5341 taacaggcta tgaacaggcg cttaaacatt acaaacttac cactgacaac aatcgcatct
     5401 actttgccga cgagtttctg gaagaaaagg gctataaatt tagcaagcga ttattcaagc
     5461 acgatccaca aattgatgct atcatcacaa ccgatagcct cctagctgaa ggtgtttgta
     5521 actatattgc caaacaccag ctggatgtcc ctgttctcag ctttgactcg gttaatccca
     5581 agctcaactt ggcagcctat gtcgatatca atagtttaga gcttggtcgt gtttcccttg
     5641 aaactattct ccagattatt aatgataata aaaacaataa acaaatttgt taccgtcaat
     5701 tgatcgccca caaaattatc gaaaaataag agactgggca aaaagtcgtt aaaagcaaaa
     5761 acgcatagta tcaggtattg aaaaaacttg atactatgcg ttttaatgtg ggaagattta
     5821 cttccttttc tactgaaatt gagtcttttc ccaagatctt tttatactca atgaaaatca
     5881 aagtgcaaac taggaagcta gccgcaggtt gctcaaaaca tggttttgag gttgcagata
     5941 gaactgacga agtcagctca aaacatggtt ttgaggttgt agatgaaact gacgaagtca
     6001 gctcaaaaca tggttttgag gttgcagata gaactgacga agtcagctca aaacatggtt
     6061 ttgaggttgc agatagaact gacgaagtca gctcaaaaca tggttttgag gttg
//
LOCUS       DAVLSL010000110         6110 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLSL010000110 DAVLSL010000000
VERSION     DAVLSL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.112"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..613
                     /locus_tag="VXN30_001616"
                     /pseudo
     CDS             1..613
                     /locus_tag="VXN30_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(847..1299)
                     /locus_tag="VXN30_001617"
     CDS             complement(847..1299)
                     /locus_tag="VXN30_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358217.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYAIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     AKDRSILDNHLDHLLEKMVNDPEKLADFTMDKSLDDAIDEAKSQITFK"
     gene            complement(1289..1588)
                     /locus_tag="VXN30_001618"
     CDS             complement(1289..1588)
                     /locus_tag="VXN30_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358218.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLKVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            2261..2575
                     /locus_tag="VXN30_001619"
     CDS             2261..2575
                     /locus_tag="VXN30_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV1358219.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2591..2977
                     /locus_tag="VXN30_001620"
     CDS             2591..2977
                     /locus_tag="VXN30_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV1358220.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            3006..4391
                     /locus_tag="VXN30_001621"
     CDS             3006..4391
                     /locus_tag="VXN30_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1358221.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            4394..4546
                     /locus_tag="VXN30_001622"
     CDS             4394..4546
                     /locus_tag="VXN30_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV1358222.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4569..5987
                     /locus_tag="VXN30_001623"
     CDS             4569..5987
                     /locus_tag="VXN30_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV1358223.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMEI
                     RLRSKLKSVLIVHYLKILYNRN"
     gene            6036..>6110
                     /locus_tag="VXN30_001624"
     CDS             6036..>6110
                     /locus_tag="VXN30_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEV1358224.1"
                     /translation="MLVFQIRNVDKTSTVLKQTKNSDYA"
BASE COUNT         2021 a         1111 c         1236 g         1742 t
ORIGIN      
        1 attatagaac ttttagactg taatcaaaca acgatttggc gaaatgtaaa aaaatatgag
       61 gagttcggac tcgactccct ccttcaagaa acacgtggtg gtcgtaacca tgcatatatg
      121 acagttgagg aagagaaagt ctttcttgcc cgccatttga aggctgcaga ggcaggagaa
      181 tttgttacaa ttgatgcctt atttcaggct tataaaaagg agttaggtcg ttcctacaca
      241 cgtgatgcct tctatcaact gttgaagcgc catggttggc gaaatattac gccacgtcca
      301 gaacatccta tcaagaagaa aagaaagcgc tttaaaacca gtagatgttt tcataaggtt
      361 cgcttgatgt accaagatga agctggtttc ggtagaatca gtaaactggg atcttgttgg
      421 gctccaatag gagtaggtcc acatgtccat agtcactata tacgagaatt tcgctattgt
      481 tatggagctg ttgatgccca tattggaaga tgtcatgaat caactccaag atgtcataca
      541 aggactggag aaggaggtga taaagtccat cgttaatcgg agatggacta gaatgctttt
      601 tgaaaacaga tgagtattag aaataaatga aaagtcaata tcaaaacaat cagaatcttg
      661 atgatgctaa attcaaaaaa actacataat aattttaata agggtaagtc gtaccaagaa
      721 accccaccaa gataaaaact tagtggggtt tggcggggaa tataattatt ctcccaatta
      781 ttatcgcctt tatattatct caactctcat taaacaagag ttccgcaatc aaattgcgat
      841 actctattat ttaaaggtaa tttgggattt agcttcatca atagcatcat ctaatgactt
      901 atccatggta aaatcagcta atttctccgg atcattaacc attttttcta aaagatggtc
      961 taaatgattg tctaaaatcg aacggtcttt cgcgtagaaa tgtagagaat ccccttgcca
     1021 agcgatggct aaaggaatct cttctttttc taaaaaagct ttaaatttct ctatatcaat
     1081 tggtttgtct aaaaaatctt ttttcagatt aatcgtatca atcgcatagg gagattgtag
     1141 caattcttct aatttctgca ctcctatctt ataggcggaa tcctgtgcta aagcctgacg
     1201 tctagaccat tctagaatct ttaaaagact ttttacagta aataaaagac tacgctctgc
     1261 atattgaact gccattcgtt cctgttgttc agaggacatc tgatgcctcc ttcttcacaa
     1321 atagcagctt tccttcttta taacgataag ctatcaattt ctgacgttgc ttaatcgact
     1381 tgacaacctt cagtagatct ctcgaataag gttctcgaag agtgacttct actgccttcc
     1441 catcaacgat accaacacgt ttaaattcaa tgtagtcttt tttgagatgt cctaagccca
     1501 taccaaaagg aaagtgatga atcaattgga tggtacaacc acctttattt cccatttgtt
     1561 tcaaatcata caagtttact accttcatga ttaactcctt tatctagttt gtcgcatcgt
     1621 ttaagtctgg taacgataaa ctccgtgtct gttagaaaat tctcacacac gtcttgtgcc
     1681 agttgccctt cacagggaaa tactctcagt ccctacttac acaggcacgc taatcaagac
     1741 ggagtggatt caattttcaa agaacaggta gctttattat agagaagagt agttgaaatt
     1801 tttatcacat tttttatttt tctgtagagc taaattcata ctaaacaaag attttgaaat
     1861 aaaagactaa cattgttaca taacgttagc atttcatttc cctaaaataa gctaattttg
     1921 tctaaaaaat agcataaaaa tctagttatc cgcataaaaa ctggacttat cacactttat
     1981 caaggtcaaa accactcaat ttactactaa tttactactt atgaatgagc tttgatacga
     2041 cgatttatcc ttgaaaagtg aagatataaa gatacttcca ataaaatttg aatatttaat
     2101 aggtagacac ttcaaaaaat gaggtgtcta tttttttacc cgattttgaa aggaagtgaa
     2161 cttatgaaaa caaaaaatca agaatcaaaa ggtcgttccc cactctttaa gaccatcaaa
     2221 cattcattca gccaataaaa aagaaaggat aggtaaaaat atggaactta aatttgtgat
     2281 tcccaacatg gaaaaaacat tcggcaattt agaatttgct ggcgaggata aagtcgttca
     2341 gcgaagaatc aacggacggc taactgtctt atcaagaagc tataatctct attctgatgt
     2401 tcaaagagca gatgatattg tggtggtgct tcctgctgaa gctggcgaaa aacatttcgg
     2461 ctttgaggaa cgtgtgaagt tagtcaatcc acgtattacc gcagagggct acaaaatcgg
     2521 cactcgtggt tttacaaatt accttttaca tgctgacgac atgataaaag aataaagaaa
     2581 gagaggaaaa atgatgagat tagcaaatgg cattgtatta gataaagaca cgacttttgg
     2641 agaattgaaa ttctctgctc tacgtcgtga agtgagaatc caaaatgaag acgggtcggt
     2701 ttcagatgaa atcaaggaac gtacctatga cttaaaatcc aaaggacaag gacgcatgat
     2761 tcaagtaagt attcctgcca gcgtgccttt gaaagagttt gattataacg cacgggtgga
     2821 acttatcaat cccattgcgg acaccgttgc tactgccacc tatcaaggag cagatgttga
     2881 ctggtatatc aaggcagacg atattgtgct gacaaaggat tctagttcat tcaaagctca
     2941 accacaagca aagaaagaac cgacacaaga caaatagtcg ctaggtagaa aggagacttt
     3001 ttcgcatgaa acagcgtggt aaaaggattc gcccatctgg taaagattta gtctttcatt
     3061 ttacgatagc gtcactcctg cctgttttcc tgctggttgt cggactgttt catgtgaaga
     3121 caatccagca gatcaactgg caggatttta acctatcaca agcagataag attgacattc
     3181 cctatttaat tatcagtttc agtgtcgcaa ttcttatctg cttgctggta gcgtttgtat
     3241 tcaaacgggt tcgctatgat acggttaaac aactttacca ccgtcaaaaa ctggcaaaga
     3301 tgatacttga aaacaagtgg tatgaatctg aacaggtcaa aacagagggt ttctttaaag
     3361 atagtgctgg tcgtacaaag gaaaagataa cctacttccc taaaatgtat tatcgactta
     3421 aaaatggctt gatacagata cgggtggaaa tcacgctggg aaaatatcaa gaccaactct
     3481 tacacttgga aaagaaatta gagagtggct tgtactgtga gctgacggat aaagagttaa
     3541 aggattccta tgtggaatat actttgctct atgacaccat agccagtcgt atttctattg
     3601 atgaagtaga agctaaagat ggtaaacttc gcttaatgaa aaacgtatgg tgggaatatg
     3661 ataagctccc tcatatgttg attgctggtg gtacaggtgg cggtaaaact tactttatac
     3721 tgacactgat tgaagccttg cttcatacag attcaaaact gtatattctt gacccgaaaa
     3781 atgctgacct tgcggactta ggttctgtga tggcaaatgt ctactataga aaagaagact
     3841 tgctttcttg cattgaaaca ttctatgaag aaatgatgaa acgtagtgag gaaatgaagc
     3901 agatgaagaa ctataagact ggcaaaaatt atgcttactt aggtctcccg gcacacttct
     3961 taatctttga tgaatacgtc gctttcatgg aaatgctggg aacaaaagaa aacaccgcag
     4021 ttatgaataa gctgaaacag attgtcatgt taggtcgtca agctggcttc tttctaatac
     4081 tggcttgtca acgtccagac gcaaaatatt taggcgacgg aatccgtgat cagtttaatt
     4141 tcagagtggc tttaggtcgt atgtctgaaa tgggctatgg catgatgttt ggcagtgacg
     4201 tacaaaagga tttcttctta aagcgaatca aaggtcgtgg ctatgttgat gtaggaacaa
     4261 gtgtcatatc agagttttat actccccttg taccaaaagg atatgatttc ttggaggaaa
     4321 ttaaaaagtt atccaacagc agacagtcca cgcaggcgac gtgcgaagcg gaagtcgcag
     4381 gtgtggactg atcttgctgg ctggtgtggc aatagccacg ccagcactta accccccgta
     4441 tctaacaggg gggtacaaat cgacaggaaa cagtcaaaaa aacattagaa aatcctttgg
     4501 ttacaaggga tttacaaaat ttcagcgtat gtcaaatggg ctttaaaagt tgacatacgc
     4561 ctttttgatt ggagggattt ttactgaatg aacaaacttg gttacagcat ttaaaagaaa
     4621 aacgcttggc ttatggacta tctcaaaacc gtttagctgt tgcgactggt attacaaggc
     4681 agtatctaag cgatattgaa acaggaaaag tcaagccatc agaggattta cagcagtccc
     4741 tttgggaagc tctggaacgc ttcaatcccg acgctcccct tgaaatgctg tttgattatg
     4801 taaggattcg ctttccgaca acagacgtac agcaggtggt cgaaaacatc ttacaactga
     4861 aactgtccta ttttcttcat gaggactatg gtttctattc ttattcagag cattatgctt
     4921 taggcgacat attcgtcctt tgctcccatg aactggacaa aggagttctg gtggaattga
     4981 aaggtcgtgg gtgcagacaa tttgaaagct atcttctggc acaacaaaga agctggtatg
     5041 agttctttat ggacgttttg gtggctggcg gtgtgatgaa acgccttgac cttgccatta
     5101 acgataagac agggattttg aatatccctg tactcactga aaagtgccaa caggaagaat
     5161 gtatctccgt cttccgcagt tttaaaagct atcgcagtgg cgaactggta cgcaaagagg
     5221 aaaaggaatg tatgggaaac accctctata tcggttcatt acaaagtgaa gtttatttct
     5281 gtatctatga aaaggactac gagcagtaca agaaaaatga tattcccatt gaagacgcag
     5341 aagtaaaaaa ccgttttgag attcgattga aaaatgagcg tgcctattat gcagtccgtg
     5401 atttactcgt ctatgacaat ccagagcata ccgcctttaa aattatcaat cggtatatcc
     5461 gttttgtaga taaagacgat tccaaacctc gttctgattg gaaactgaat gaagaatggg
     5521 cttggtttat tgggaacaat cgtgaacgat taaaactaac cacaaaacca gagccttact
     5581 ccttccaaag gacgctgaac tggctatctc atcaagttgc cccgacctta aaggttgcga
     5641 ttaaacttga tgaaatcaac cagacgcagg ttgtaaaaga cattctcgac catgcgaaac
     5701 tgacagaccg acacaagcag attttgaagc aacagtcagt aaaagaacag gacgtgataa
     5761 caacaaaaaa aggatatctg tcaaccatac cagttgacag atatccaaaa aaagatataa
     5821 tgggagataa gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag
     5881 caaagaatgg cggaaacgta aaagaagtta tggaaataag acttagaagc aaacttaaga
     5941 gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt taaattagat
     6001 gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa atacgtaatg
     6061 tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
//
LOCUS       DAVLSL010000111         6026 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLSL010000111 DAVLSL010000000
VERSION     DAVLSL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.113"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            179..1047
                     /locus_tag="VXN30_001625"
                     /pseudo
     CDS             179..1047
                     /locus_tag="VXN30_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1348..3237)
                     /locus_tag="VXN30_001626"
     CDS             complement(1348..3237)
                     /locus_tag="VXN30_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1358225.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            3375..4940
                     /locus_tag="VXN30_001627"
     CDS             3375..4940
                     /locus_tag="VXN30_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1358226.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4940..5680
                     /locus_tag="VXN30_001628"
     CDS             4940..5680
                     /locus_tag="VXN30_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1358227.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(5789..6004)
                     /locus_tag="VXN30_001629"
     CDS             complement(5789..6004)
                     /locus_tag="VXN30_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358228.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         1851 a         1370 c         1103 g         1702 t
ORIGIN      
        1 ggattagttc atttcctcca atcgcttgac gatttccgaa agggtctaac aacagctttt
       61 tgtttcaatt ggtaatgagt ttgataacgc atagacgttt tatctcgtct atggtaacaa
      121 ggcaaaaagt tcgtggataa ccaagtagac ttgaaatact atatctggag gtactcccat
      181 gctttatgtg ggcattgatg tcgctaaaaa taaacacgat gttacagcct tgaatgttcg
      241 aggaaaactg ttcttaaacc actcactttt tcaaataata aagctggttt tgaactctta
      301 gatctgtctt ttcgacagct caaccaagac tgtctcatcg ctcttaaact tctttctgac
      361 cccaatcgtg aacaatttca acacgataat cggcaaatag aactaaaaat actggctaga
      421 catattcatc gtctcaagaa aaaacagtct gattggaaag tacaatacac tcgttgtctt
      481 gatatcatct ttcctgagtt ggataaaatc gttggaaagc attcagaata tacctaccaa
      541 ctcttgacac gctaccctaa tcctcagaaa aggcttgagg caggatttga taagctgata
      601 gaaattaagc gattgaccgc ttctaaaatt caggatatcc tctcagtcgc acctcgttct
      661 atcggaacaa catctcctgc tcgtgaattc gaaatcatcg aaatcatcaa acattacaag
      721 aggctcattg acaaggcgga aacatgtgtc aatgacttga tggctgagtt caactcggtc
      781 atcacgacgg ttactgggat tgggggtcgt ttaggggcgg tcattttagc cgagattcga
      841 aatattcatg cctttgataa tcctgctcaa ttacaagctt tcgctggact ggattcttct
      901 atttatcagt caggtcagat tgatttagct ggaagaatga tcaaacgggg ttcccctcat
      961 ctgcggtggg cactcataca agctgccaaa gcatgcgctc gcttttcacc tgcttttaag
     1021 gcctatctta agactaagtt agaataagga aaacattaca atgtagccat catccacctt
     1081 gcaaaaaaac ttatccgaac cctgttttat atcctaaaaa agagctgcca tttgacgaac
     1141 aaaaagtgat gtaatttctg aaattaacac aggattacct ttgaaaaaat ggactttttc
     1201 aagggctttt tgccgtgctc taaaatcatc gccaaatcat caaattgaaa taagaaatag
     1261 tttagataaa actattgact tttcttatag tttgtctttc ttctatcttc cattatatca
     1321 tactttttca gtctatggta gctctcttta aaaggctccg atactgccgc cacctccgcc
     1381 tccagagaag ccaccaccag aacttccact tccagaagat acagagtagg tacttgctgt
     1441 atttgcgaca ctagcataat ggctcatttg cgcgcttgaa tgataaaaca tactgtgcca
     1501 gccataagct acatagagat tgatatctgg attttcaact tgaatctgat gaaccttcat
     1561 caaatgacta accttgtccg catagccaaa taaggttgca tagaccaaga gacgattcca
     1621 aacaacaata ctttccaatt cagccttatc cagtcgtgca atctcacgta acatattttc
     1681 aaaactggtc cagagatagt agacctcagc tcccgcttca tttagaacac catcacgatt
     1741 atctagtcga agcttccaat aatagaaaac agacaaaact aaccctagaa aaccaagtat
     1801 tggcaaaggg aggtaaagat agccatgaac gtctaaactg tacaagaaca aaccaaatcc
     1861 gataaatagg ggcaagatag tcaagacacc catacccact tgcaaagcct tttccaaacc
     1921 agttaaagga cgatagtaat ctgggagtcc ccagaagaca actcgctttc tcactccttc
     1981 ttgcatctct tttaatactg cttcgaaaga agatttgagc tgaatccctc ttgcttgaat
     2041 ccgtttctca tcagcagctt tagctccgcg ataaaggctg tcagatacct tgtaatccgc
     2101 aaacaaattg gaaagagtgg cttctttttt gcctgaaaag gctagattta gacagtctct
     2161 ctcaaatctt gacaaaccat cttctttcac caatctcaag ccaactgcat ctccttctga
     2221 aataatagaa acattcccac ggtccatcac atctagcaag gtagcttgaa taagttggtc
     2281 aaaagtgaat ttgccagccc ccttgactaa gggactcact tcctccaagg aggtcgagta
     2341 gacagcctct gataaaacca taggctctaa ttccattggt ggttcataga gacgatgatt
     2401 tttggcatat ttgactgaag gagtggtctt tcttctataa ataaaataga agcagacact
     2461 caataacaag gagatagaaa ggatcgaagg gaggacccaa gtaacaagtt gtttactctg
     2521 atctttttct ctaacaatcg agtcttctat cttattaaac tcttctaaac gattcccttt
     2581 caatccctga tccctagcgc tagcaaaatc ggtccgaggc cagtaggcat gcaactcaac
     2641 tccacgctta gccggaagat tgtctaaacg gatagtataa tcaaggttac tcttttcaat
     2701 cgttccctct ctaaaaagtt tccctgtatg gaaaaagagt ttttcagccc ccttgtctcc
     2761 ccttacatga aattcaaact tttcaataga ctctgaacta tctgtcagag gttgccaatt
     2821 taattcagcg atatcatcat aaaggaaaag taaatttttt aagttccaga cgaggtcaac
     2881 ttcaactatg tcgccctcct gacctggatt atagactctc acagtataac catccgcttc
     2941 ttctgttact tcgctagtca catctgctag ttctgcaccg tttttcgcgg cctgaacctt
     3001 tggatgaggg tcaatgtcaa acccgctagg catcttacca gcacgtccaa gtcccacgat
     3061 ttgtccctta aagtcctcct caaactggta aactatcttc tgtctaaact ctgccgtatt
     3121 atctgcatga atatacaaat ccccttgata agagtttatc ttgaaatcaa tggcaaaaac
     3181 agagagtgga agaaggcaaa acaagcctaa caccagtaag aaaaaagttt ttttcatcaa
     3241 ctaagccccc tttttatctt tcttatcatt atagcatttt ttcgtaagta aaggcttact
     3301 tatgttgaaa aattacgcat ttttcgataa aataggagac agttattttt taatagatca
     3361 gaataggaga aatcatgaga aaaatatact tatctatttt cacaagtctc ttgctgatgc
     3421 taggacttgt caatgttgct caagccgatg aatatttacg catcgggatg gaagcagcat
     3481 atgctccctt taactggacc caggatgatg atagcaacgg agctgtcaaa atcgatggga
     3541 ccaatcagta tgccaacgga tacgatgttc aaatcgccaa gaaaatcgct aaggacttag
     3601 gtaaagaacc tttggttgtt aaaaccaagt gggaaggtct agtccctgcc cttacttctg
     3661 gtaagattga catgattatc gcaggtatga gtccaactgc agaacgcaaa caagaaattg
     3721 ccttttcgag cagttactac actagcgaac cagttttgct tgtcaaaaaa gattctgcct
     3781 acgcaagtgc taaatctttg gatgacttta atggtgcgaa aatcacttct caacaaggtg
     3841 tctacctcta cgacttgatt gcacaaatcc caggtgctaa aaaagaaaca gccatgggag
     3901 acttcgctca aatgcgccaa gctcttgagg ctggtgtcat tgatgcttat gtttctgaac
     3961 gtccagaagc actgactgct gaagctgcga actctaagtt caagatgatt caagtagaac
     4021 ctggtttcaa aactggggaa gaagatacag ctatcgctat cgggcttcgt aaaaatgaca
     4081 atcgtattag ccaaatcaat gccagcattg aaaccatttc aaaagatgac caagttgcct
     4141 tgatggatcg tatgatcaag gaacaacctg ccgaagctac aacaactgaa gagactagca
     4201 gtagtttctt tagccaagtc gctaaaattc tttctgaaaa ctggcaacaa ctcttgcgtg
     4261 gtgctggtat cactctttta atctctatcg tcggaaccat cataggtctc attattggac
     4321 ttgccattgg tgtcttccgt actgctcctc tctctgaaaa caaagccatt tatggccttc
     4381 aaaaactagt cggctgggtt ctcaatgtct acattgaaat tttccgtggt acgccaatga
     4441 ttgttcaatc gatggttatc tactatggaa ctgcccaagc tttcgggatc aaccttgacc
     4501 gtacactggc tgctatcttc atcgtttcaa tcaataccgg tgcctacatg actgaaatcg
     4561 tccgtggtgg tatcctagca gttgacaagg gacaatttga agctgcgact gctcttggta
     4621 tgacccataa ccagaccatg cgtaagattg tcctacctca ggtagtccgt aacatcctac
     4681 ctgcaactgg taatgaattt gtcatcaata tcaaagatac atctgtattg aacgttatct
     4741 ctgttgtcga actttatttc tcaggaaata ccgtggcaac acaaacctat caatacttcc
     4801 agacatttac aatcatcgcc gtgatttact ttgtcctcac cttcaccgta acacgtatcc
     4861 tacgatttat cgagcgccga atggacatgg atacctatac tacaggtgct aaccaaatgc
     4921 aaacggagga tttgaaataa tgacacaagc aatccttgaa attaaacacc tcaaaaaatc
     4981 ctatggacaa aacgaagtgc taaaagacat ttcactcact gtccacaagg gagaggtcat
     5041 ctctatcatc ggaagctctg gaagcggaaa atcgaccttc ctacgctcca ttaacctact
     5101 tgaaacacca actgatggac aaatccttta tcatggacaa aacgtcctcg aaaaaggcta
     5161 tgacctcacg caataccgtg aaaagttggg gatggttttc caatccttta acctctttga
     5221 aaatctcaat gttcttgaaa acacaatcgt cgctcagaca actgtcctaa aacgcgaacg
     5281 cacagaagct gaaaagattg ccaaagaaaa cctggaaaag gtcggcatgg gagaacgcta
     5341 ctggcaagcc aaaccaaaac aactctcagg tggtcaaaaa caacgtgtgg ccatcgctcg
     5401 tgccctctcc atgaatccgg acgctattct ctttgatgaa ccaacatcag ctctcgatcc
     5461 agaaatggtt ggagaagtcc tcaaaatcat gcaggacctg gctcaggaag gcttgaccat
     5521 gattgtcgta acccatgaaa tggaatttgc ccgtgatgtc tctcaccgtg ttatctttat
     5581 ggataagggc gtgatcgctg aagaaggtaa accagaagac ctctttacca atcctaaaga
     5641 agaccgaaca aaagagttcc ttcaacgcta tctcaaataa aaagaaaagg ctgcatcaac
     5701 cgtgcagtct ttttgctgtc ctcaaaatga aaaggcagat tctctatctc tactacttga
     5761 aggtcaagaa tccgccgtta tccaaagatt aatcttcaaa tttttcataa tttttttcat
     5821 agaaatcaat taagcctagg gctgtttcaa atgaaatatt ttttactttt gcacgaccct
     5881 gcgccagagc aatgatagac atttcacgcg cattcgtttc tttagagata cggtagcctg
     5941 tgattttctt atcacgaacc cagccaacaa ctgattctac tttttcaaag tttgacttag
     6001 ccatgttttt ctcctatttt cttctt
//
LOCUS       DAVLSL010000112         6021 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLSL010000112 DAVLSL010000000
VERSION     DAVLSL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.114"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            128..1897
                     /locus_tag="VXN30_001630"
     CDS             128..1897
                     /locus_tag="VXN30_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV1358229.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1921..3876
                     /locus_tag="VXN30_001631"
     CDS             1921..3876
                     /locus_tag="VXN30_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1358230.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECMKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3878..4315
                     /locus_tag="VXN30_001632"
     CDS             3878..4315
                     /locus_tag="VXN30_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1358231.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNENYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNESNTQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4377..5513
                     /locus_tag="VXN30_001633"
     CDS             4377..5513
                     /locus_tag="VXN30_001633"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV1358232.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIDQIVEYI"
BASE COUNT         1841 a         1006 c         1235 g         1939 t
ORIGIN      
        1 taatttttaa gcctttttgc ccagcctcgt caaaagtaat gttttgacac aaaatctgtg
       61 acaaaacttt agttttaaag gtttttaact ttgtatatac tagttttaag aaaaggagga
      121 tgatctaatg gaagaaaaag tatcattgaa ggtcagggtt caaaaactag ggacatcgct
      181 ttcaaatatg gttatgccca atattggagc atttattgct tggggagtat tgactgccct
      241 ctttatcgct gatggctatc tgccaaatga acagttagct actgttgttg gtcctatgtt
      301 aacgtattta ttgccaatcc tgattggtta cacaggtgga tatatgatcc atggccaacg
      361 tggtgccgtt gtaggagcta ttgctactgt tggtgcaatc acaggttcta gtgttcctat
      421 gtttatcgga gctatggtaa tgggcccact gggaggatgg actatcaaga aatttgatga
      481 gaagttccag gaaaaaattc gtcccggatt tgaaatgtta gttaataact tctcagctgg
      541 tctcgttggt tttgcattat tgcttttggc tttctacgca atcggtccag tcgtatcgac
      601 tcttactgga gctgttggga atggtgttga ggctattgtc aatgctcgcc tccttcctat
      661 ggctaatatt atcatcgaac cggctaaagt ccttttcctc aataatgccc tcaatcatgg
      721 catttttact cctctgggag tagaacaggt agctcaagct ggtaagtcaa ttctcttcct
      781 attggaagct aatcctggac caggcctggg aattctatta gcttatgctg tattcggtaa
      841 aggttctgct aaatcttctt cttggggggc aatggttatt catttcttcg gagggattca
      901 tgaaatttac tttccttatg ttatgatgaa gcctactcta tttttagctg ctatggcagg
      961 aggtatctct ggaactttta cttttcaact cttagacgct ggtcttaaat ctccagcttc
     1021 accaggttct attattgcga ttatggctac ggcgccaaaa ggtgtttggc ctcatctaaa
     1081 tgttctttta ggtgttttag tggcagcagt tgtttctttc cttgtagcag cccttattct
     1141 tcatgcagac aagtcaactg aggattcgct cgaagctgct caggcggcta cccaagcagc
     1201 taaggctcag tctaaaggtc agttagtatc aacttctgtt gatgcagttg tttcgacaga
     1261 ctcagtggaa aaaatcattt tcgcctgcga tgctggtatg ggaagctctg ctatgggagc
     1321 tagtattctt cgagataagg ttaaaaaagc aggtctagag attccagtat ctaatcaggc
     1381 aatctcaaat ttgcttgata caccaaaaac attaattgtt actcaggaag aactgacacc
     1441 aagagctaaa gacaagagtc caagtgctat tcatgtttct gttgataatt tcttagcgtc
     1501 ccctcgttat gatgaaattg tagcttcatt aacaggagct tctccaatag cagaaattga
     1561 aggagatata ccaacttcag caccagtaga tagtcaggaa attgacctta accatattga
     1621 tgctgtagta gttgcttatg gtaaagcaca gggaactgca actatgggct gtgaaacgat
     1681 tcgggctatc tttagaaaca agaatattcg tattccagtt tctactgcca aaatttcaga
     1741 attaggtgaa tttaattcta aaaacataat gattgtaaca actatttctt tacaggcaga
     1801 agtgcagcaa gcagcaccga attctcaatt tcttattgtg gatagtttag taacaacacc
     1861 agaatatgac aaaatggctg ctagaatgta caaatagaac tagaggtttc taaattacga
     1921 atgctattaa ccaaacgaga agaacaatta ttgaaggctt tcctacatgt agggaagctt
     1981 tcaatgcaag atatgactga aatcttacag gtttcatcta gaacaattta tcgaacttta
     2041 tcagatttga cagatagcat ggagcaatat ggaatcgaaa taacgaagca tgggaaatac
     2101 tatattttga ctggagagtt ggatgatttg ccgacagaac ttgaagtgtt agttgagtat
     2161 agtccccaag aaagacaaga gttgattacc tatcgccttc tgactgagag tggttttgtc
     2221 accaatgaag cattgcaaga gtgcatgaaa gtcagtaatg taactattat tcaggatatt
     2281 tcagatattg ataagcgtct tttagacttt gatctgaaaa ttgaacgaca aaaaggttat
     2341 cggatttctg gtgattcagt tggtaagaga agatttttgg ctattttact gacaaactgt
     2401 atctcagtag cagatttttc aaccggtaat tttgggagct ttgatatttt agaagcagat
     2461 agaactaggc tggccagtca gattgttaat aagcaactgt caggttttcc agatatggat
     2521 gctaggatga agatgttttt tgcgatcttg ttatctctta taggtcagga gcaaaacatt
     2581 gaaaattcac ctaatactag taagcaggct ttggaaattt ctcaaaaaat ttttcaagct
     2641 tactctaagc agactgcaca attttatagt attcaggaaa ttatctattt tgcgagcatc
     2701 ttggatgaat taatcattaa acgtcaggac aatccgctct ttacggagaa atttgatggt
     2761 gaatttttct acaatatttc aaatctgatt gatacggttt ccatgtatac caagattgac
     2821 ttttttaagg acaaggtttt attcaatttt cttttccatc atattcggct cagtttaggc
     2881 gtccctatcc tttttcaggg tgaaaatttg ccagaatctg tccagatttt agttgaaagg
     2941 aataaatttc tttatacagt catcagtctt ttagtgaatg atatttttcc gaaatatctt
     3001 catacagagt atgagtatgg catgattgcc ctacatttta tctctagctt aggtcgtagt
     3061 ccagagattt atccagtccg tgttttgctt ttaacggatg aacgtcgggt cactagagat
     3121 ttattagtca gtaaaattaa gagtgttgct ccttttgtag agttgataga tattcagtct
     3181 ctagtagatt accacagtat tgatctcagt cagtatgatt atattttatc taccaagccg
     3241 ctgactaatc aggaaatcga tgtaatttct agttttccaa ccgtcaaaga attgcttgaa
     3301 ttacaggaac gacttcagta tgtacaggca catcgtacaa ttgtcgcgcg tgatgctatc
     3361 gctccagaga aaagttatga cttgcaagat tatttaatat ctagtagtca gcttttgagt
     3421 caattcgagt tggttcaatt ggagaataat caatcatttg agcacacggt agaacaaatc
     3481 atccaatatc agaagaatgt gagtgacaga gattacctaa caagaaaatt gttatctcac
     3541 ttccagaata gtcctatggc tattcctaat actggtctgg tgcttttaca tagtcagtct
     3601 agcaaagtaa caacaaatag ttttactatg tttgaactca aactacctat ctccgcattg
     3661 tcaatgaaac gagaggaaga agaggtcaaa aggtgtctgc taatgctaat gtctaaagaa
     3721 gctagcgagg aagcgagaga tttaatgaca gctattagtc agtcgattat tgaaaatcat
     3781 ctttatacag agatttacaa gacgggaaat caatccatta tttatcagat gctaaatact
     3841 atttttaacg aaaaaattaa gaaattggag aactaatatg aaacttgaaa aacatttgat
     3901 taagcttaat aaacaatttt ctaacaagga ggaagctatt tgttattgtg ggcaagttct
     3961 ttatgagggt ggatatgtta atgaaaacta tattgaagcc atgattgagc gtgataaaga
     4021 gctatctgtt tacatgggta actttatcgc cataccgcat ggaacagatg cagcgaaaaa
     4081 ggatgtcctc aagtctggta ttacagtcgt tcaagtccca agaggggttg attttgggaa
     4141 tgaatctaac actcaagtgg caacggttct ttttggtatt gctggtattg gtaatgaaca
     4201 cttagaaatt attcagaaaa tttctatctt ctgtgcagat gtggataatg ttcttaaact
     4261 agcagatgct cagtcaaaag aggaagtatt gcgcttattt gatgctgttg aataattgaa
     4321 tttcgtcatt tgtcatctag tatatatgtc cctcaaatag gaaaaggaga aattgaatga
     4381 aacattctgt tcattttggt gccggtaata tcggtcgtgg ttttataggt gaaattctat
     4441 ttaaaaatgg tttccatatt gattttgtgg atgtcaataa tcagataatt catgctctaa
     4501 atgaaaaggg caagtatgaa attgaaattg cacagaaagg acagtctcgt atagaagtaa
     4561 ctaatgtggc tggcattaat agcaaagaac atcctgagca agtcattgaa gcgattcaaa
     4621 agacggatat tattactact gcaatcggac ctaatatact cccttttatc gctgaacttc
     4681 tagccaaagg aatcgaagct cgccgagttg cagggaatac acaggtattg gatgttatgg
     4741 cctgtgaaaa tatgattggc gggtctcaat ttctttatca agaagtcaag aaatatttaa
     4801 gtccggaagg tttgacattt gctgataact acataggttt tccaaatgct gcagtagaca
     4861 ggattgttcc agcacaaagt cacgaagatt ccctttttgt tgtggtcgag ccctttaatg
     4921 aatgggtcgt ggaaaccaag cgtcttaaaa atccagattt acgtctaaaa gatgtgcatt
     4981 atgaagaaga tttagaaccc tttattgagc gaaaactttt ttcagtcaat tctggacatg
     5041 caacttcagc ttacattggt gcgcattatg gtgccaagac aattttggaa gctcttcaaa
     5101 atcctaatat caaatctcgg attgaatctg tattagctga aattcggagt ctcttgattg
     5161 ccaaatggaa ctttgataaa aaagaattgg agaattatca caaagtcatt atagaacggt
     5221 ttgaaaaccc tttcatagtg gacgaggtta gtcgcgtagc tcgtactcca atccgaaaat
     5281 taggctataa tgaacgattc atccggccga tacgtgaatt gaaagaactc agtttgtcat
     5341 ataaaaacct acttaaaaca gttggctatg cctttgacta tcgcgatgta aatgatgaag
     5401 aaagtattcg attaggtgaa ttgttggcta aacaatcagt caaagatgtt gttatacaag
     5461 ttacaggttt agacgaccaa gaattgattg atcaaattgt agagtatatt taatattttt
     5521 cgaaaatctc ttcaaatcag gttagcatcg ctttgtctta ggcatatgtt actgacttcg
     5581 tcagttctat ctacaacctc aaagcagtgc tttgagctga ctccgtcagt cttatctgca
     5641 acctcaaaac actgtttgag ttatctgcgg taatctttct agcttgtctt tgatttttgt
     5701 tgttatttat aaggtaaaag aagctggaca aaaagtcttc aaaatcggga aaaggcagcc
     5761 tatcgggtgt tcaaaaatct tgataggatg tcctttatta tggaaagcct tattggattt
     5821 tctcctcaga ttgagttttt gatcagcttt atgagatagg ttttgctaga gatgtagccc
     5881 atcatgttat ttttatggac tgtgggaaaa ttgttgaaaa aaataatgcc catcaattct
     5941 ttagtcgtcc aagagaagaa cgaaccaagc aattttggaa cgaattcttt cgaatgcgat
     6001 ctatatagta aaatgaaaca a
//
LOCUS       DAVLSL010000113         6010 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLSL010000113 DAVLSL010000000
VERSION     DAVLSL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.115"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            25..870
                     /locus_tag="VXN30_001634"
     CDS             25..870
                     /locus_tag="VXN30_001634"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1358233.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1017..2078)
                     /gene="dinB"
                     /locus_tag="VXN30_001635"
     CDS             complement(1017..2078)
                     /gene="dinB"
                     /locus_tag="VXN30_001635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1358234.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            2426..4750
                     /gene="pflB"
                     /locus_tag="VXN30_001636"
     CDS             2426..4750
                     /gene="pflB"
                     /locus_tag="VXN30_001636"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1358235.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(5117..5335)
                     /locus_tag="VXN30_001637"
     CDS             complement(5117..5335)
                     /locus_tag="VXN30_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358236.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(5431..5669)
                     /locus_tag="VXN30_001638"
                     /pseudo
     CDS             complement(5431..5669)
                     /locus_tag="VXN30_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5662..5946)
                     /locus_tag="VXN30_001639"
     CDS             complement(5662..5946)
                     /locus_tag="VXN30_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358237.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
BASE COUNT         1780 a         1184 c         1141 g         1905 t
ORIGIN      
        1 taaatataat ataaggagaa atatatgtat cttattgaaa ttttaaaatc tatcttcttc
       61 gggattgttg aaggaattac ggaatggttg ccgatttcca gtacaggtca cttgatttta
      121 gcagaggagt ttatccaata ccaaaatcaa aatgaagcct ttatgtccat gtttaatgtc
      181 gtgattcagc ttggtgctat tttagcagtt atggtgattt attttaacaa gctcaatcct
      241 tttaaaccga ctaaggacaa acaggaagtt cgtaagactt ggagactatg gttgaaggtc
      301 ttgattgcta ctttaccttt acttggtgtc tttaaatttg atgattggtt tgatacccac
      361 ttccataaca tggtttcagt tgctctcatg ttgattatct acggggttgc cttcatatat
      421 ttggaaaagc gcaataaagc gcgtgctatc gagccaagtg taacagagtt ggacaagctt
      481 ccttatacga ccgctttcta tatcggactc ttccaagttc ttgctctttt accagggact
      541 agccgttcag gtgcaacgat tgtcggtggt ttgttaaatg gaaccagtcg ttcagttgtg
      601 acagaattta ccttctatct tgggattccc gttatgtttg gagctagtgc cttaaagatt
      661 ttcaaatttg tgaaagccgg agaactcttg agctttgggc aattgttttt actcttggtc
      721 gcgatgggag tagcttttgc ggtcagcatg gtggctattc gcttcttgac cagctatgtg
      781 aaaaaacacg acttcaccct ttttggtaaa taccgtatcg tgcttggtag tgttttgcta
      841 ctttacagtt ttgtccgttt atttgtataa gaaaaacctt gaaggggcaa ctcttcaagg
      901 ttttatactc ttcgaaaatc tcttcaaacc gcgtcagctt tatctgcaac ctcaaaacag
      961 tgttttgagc agcctgcggc tagcctccta gtttgctctt tgattttcat tgagctttaa
     1021 aatccagtca tggtaatccc caataggcgg acacctcttt ctttcttgct taattcttca
     1081 tagagttgca gggctatttg gcttatctga ctagcatctt gtgttttttg agcaagactt
     1141 tttcgtttgg taagagttga aaagtcctcg tagcggattt tcaaaatgac aatttttcca
     1201 gctttttctt gttgatgtag attgagagcg actttttctg atagaagagt cagctctttt
     1261 ttgatatctt cctcagcaag gagaatcttc ccgtaggttt tctccttgcc gattgattta
     1321 cggatgcgat tggatttgac tggagagttg tgaatgccac gagcctttcg atacagatca
     1381 tagcctagtc taccaaaacg gtctattagg gttacctcag gaacttcaag taaatcagca
     1441 ccagtaaaaa cgcccatttg atgaagacgt tctactgtct tttttcctac tccatgaaat
     1501 ttggaaatat ccatttgttt gagaaaatcc tcagcctgtt caggtagaat cactgtcaaa
     1561 ccatgtggtt tttgataatc actcgccatt ttagctaaga atttgttgta agaaacgcct
     1621 gcggaagcag ttagatggag ttcttgccag atatcttttt gaatgaggcg agcaattttg
     1681 accgctgact tgataccgag tttattttct gtcacatcca aataggcttc gtcaatgctc
     1741 atgggttcaa tcaaatctgt atagcgctta aaaatagctc gaatctggag tcccacagac
     1801 ttgtatttct cataattccc tgagataaag acagcctggg gacaacgttc ataagcttcc
     1861 ttggaactca tggcagaatg gacaccaaaa gctcttgcct cataactaca ggtagaaacg
     1921 actccccgtc cacctgtttg ccgagggtcg cttccaataa tgacaggttt tcctctgagt
     1981 ttaggattat ccctgatttc cactgcagca aaaaaggcat ccatgtcaat atggatgatt
     2041 tttcttgaca aatcatttaa caaaggaaaa atcaacatgc ctagcacctt tttatactct
     2101 tcgaaaatct cttcaaacca cgtcagctct atctgcaacc tcaaaacagt gttttgagca
     2161 atctgcggct agcttcctag tttgcttttc gatttccatt gagtgttact gcttattttc
     2221 ttttattata cccttttttc tgaaaaaaag aaaaaaggac tttatttttt caaaaatata
     2281 atacagtttg aaataaaata tagactgttt tagaaaagaa agtgtaaaaa tagggaattt
     2341 tcacttgttg aaatcggtta ctatatggta tacttgtctt atgaatgtaa cagatgactg
     2401 ttactagaaa aaagaggaca ttaatatggt tgttaagaca gttgttgaag cacaagatat
     2461 ttttgacaaa gcttgggaag gcttcaaagg cgtagattgg aaagaaaaag caagtgtatc
     2521 acgatttgta caagctaact acacacctta tgatggagac gaaagcttcc ttgcaggacc
     2581 aacagagcgt tcacttcaca tcaagaaaat tgtagaagaa actaaagcac actacgaaga
     2641 aactcgtttc ccaatggaca ctcgtccaac atctatcgct gatatccctg ctggatttat
     2701 cgacaaagaa aatgaagtta tcttcggtat ccaaaacgat gaactcttca aattgaactt
     2761 catgccaaaa ggtggtatcc gtatggctga aactactttg aaagaaaatg gatacgaacc
     2821 agacccagct gttcacgaaa tcttcactaa atatgtaaca acagttaacg acggtatttt
     2881 ccgtgcctac acttcaaata ttcgtcgcgc tcgtcacgca cacactgtaa ctggtcttcc
     2941 agatgcatac tcacgcggac gtatcatcgg tgtttacgca cgtcttgctc tttacggtgc
     3001 agactacttg atgcaagaaa aagtaaacga ctggaatgca atcaaagaaa tcgatgaaga
     3061 aacaatccgt cttcgtgaag aagtaaacct tcaataccaa gcattgcaac aagttgttcg
     3121 cctgggtgac ctttacgggg ttgatgttcg caaaccagcg atgaacgtga aagaagcaat
     3181 ccaatgggtt aacattgctt tcatggctgt ctgccgtgtg attaacggtg ctgctacatc
     3241 tctaggtcgt gtaccaatcg tattggacat ctttgcagaa cgtgaccttg ctcgtggtac
     3301 atttactgaa tcagaaatcc aagaattcgt tgatgatttc gttatgaaac ttcgtacagt
     3361 taaatttgct cgtacaaaag cttatgacca attgtactca ggtgacccaa cctttatcac
     3421 aacttctatg gctggtatgg gtaacgacgg tcgtcaccgt gttactaaga tggactaccg
     3481 tttcttgaac actcttgaca acatcggtaa ctcaccagaa ccaaacttga cagttctttg
     3541 gactgacaaa ttgccataca acttccgtcg ctactgtatg cacatgagcc acaaacactc
     3601 ttctatccaa tacgaaggtg taacaacaat ggctaaagac ggatatggtg aaatgagctg
     3661 tatctcatgc tgtgtgtctc cacttgatcc agaaaatgaa gaacaacgcc acaacatcca
     3721 gtacttcggt gctcgtgtaa acgttcttaa agcccttctt actggtttga atggtggtta
     3781 cgacgatgtt cacaaagact acaaagtatt tgatatcgaa ccaatccgtg acgaagttct
     3841 tgaattcgaa tcagttaaag cgaactttga aaaatctctt gactggttga ctgacactta
     3901 cgtagatgcc ttgaacatca tccactacat gactgatagg tacaactacg aagctgttca
     3961 aatggccttc ttgccaacta aacaacgtgc caacatggga ttcggtatct gtggatttgc
     4021 taacactgtt gatacattgt cagctatcaa atacgctaca gttaaaccaa tccgtgacga
     4081 agatggctac atctacgatt acgaaacaat cggtgactac ccacgctggg gtgaagatga
     4141 cccacgttca aacgaattgg cagaatggtt gatcgaagct tacacaactc gtctacgtag
     4201 ccacaaacta tacaaagacg cagaagctac agtatcactt ttgacaatca catctaacgt
     4261 tgcttactct aaacaaactg gtaactcacc agttcacaaa ggtgtatacc tcaacgaaga
     4321 tggttctgtg aacttgtcta aacttgaatt cttctcacca ggtgctaacc catctaacaa
     4381 agctaaaggt ggttggttgc aaaacttgaa ctcactttct agccttgact ttagttatgc
     4441 agctgacggt atctcattga ctacacaagt atcacctcgt gctcttggta agactcgtga
     4501 tgaacaagtt gataacttgg taacaatcct tgatggttac ttcgaaaacg gtggacaaca
     4561 cgttaacttg aacgttatgg acttgaacga tgtttacgaa aaaatcatgt caggcgaaga
     4621 cgttatcgta cgtatctctg gatactgtgt aaacactaaa tacctcactc cagaacaaaa
     4681 aactgaattg acacaacgtg tcttccacga agttctttca atggatgacg ccttggatgc
     4741 attgagctaa tcaagttctt gaataataaa aaggaaccct cggtcaaacg actgagggtt
     4801 ttgtgcttgg gatagtgtga gcaattcctt cggcgcaata tgcaatgttt ttgggaagga
     4861 atagtggacc gaatctgaaa tataatagat tgaaactaga atagtacacc tttacttcta
     4921 aaacattctt agaaatcgat ttgactgtcc tgatcgattt gtcctattct tatttcattt
     4981 tactatagtc cactgaaacg tctcaaacag cgctagaaat tgactggaat tctctaagtg
     5041 atttgttccg attttatttc attccatcat aaaaataaga cgactggcta attgacaatc
     5101 atcttgtttg atactgttaa taaaattcgc gaagtttatc ttgattttct ttaaaaaatt
     5161 tttctaacca atccatacca taagcataac taaaaccttc taatagaaca gagaggtcaa
     5221 tagtatcaaa tcccatgtct tttaaatatt taaaggtatc gaatggtgaa tgaaagtttt
     5281 tgtatttaat aattgcacca atatctttca aatcctgctc tctgatactc atcatcttca
     5341 ttcctaacac atactcaata gggactacta aaacggtcag attctcaaat gagtacaagg
     5401 attcgcataa acttaaggga ggttgtttgt tcatatttgc tttgcaacat gattgttgag
     5461 ccataactct tcgtggatgt taagattaaa ctgttttcct acacgagcaa taatttcatt
     5521 gattttctga ttttggtcat aaaaagcatc aacatcttgt gtggcacgta aaccatgata
     5581 ttctaagaca taaccataat tgttaaagtc agattttcct gaattaattt cttatttagg
     5641 gcttggaaga ccggtttcat tttatccatg tttgtatttc tccatcaatt tttgaacatt
     5701 ggttaactta gcaggtttat ttaatgaaat cacatattta tgagttggag tatgctcttg
     5761 aaaggcatac ttcaagctaa ttttttgaaa acgggttagt tctttggagt ctagcaactt
     5821 ttctacatct tggtttgtta aataagaata gtcttcaaaa ataacttttc gcaaatagcg
     5881 aaaccaatgg cttgcgttga attgtgattg tttggatgcc tgtatcatat aatcgtacca
     5941 atccattgtc ttcctcctat aaaatttagt ttatattatt gtagtttata ttcgaaaatg
     6001 tagtttttct
//
LOCUS       DAVLSL010000114         5963 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLSL010000114 DAVLSL010000000
VERSION     DAVLSL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.116"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            88..855
                     /locus_tag="VXN30_001640"
     CDS             88..855
                     /locus_tag="VXN30_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEV1358238.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLNKLVSGSNTLTQKSSRLTAGVG"
     gene            940..1278
                     /locus_tag="VXN30_001641"
     CDS             940..1278
                     /locus_tag="VXN30_001641"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358239.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFFAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            1306..1620
                     /locus_tag="VXN30_001642"
     CDS             1306..1620
                     /locus_tag="VXN30_001642"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1358240.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(1721..4273)
                     /locus_tag="VXN30_001643"
     CDS             complement(1721..4273)
                     /locus_tag="VXN30_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1358241.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDDSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYIIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDILKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4335..5957)
                     /locus_tag="VXN30_001644"
     CDS             complement(4335..5957)
                     /locus_tag="VXN30_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1358242.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         2004 a          983 c         1104 g         1872 t
ORIGIN      
        1 aaaattttag acaatatgtt taaaaaagga gaaactaagt ttaaagaatg gaaagcaatt
       61 taaaaaaaac caacctttat tattgtcatg atcgggattt ctcttattcc agatctgtac
      121 aatatcatat ttttgtcatc aatgtgggat ccatatgggc aattgtctga cttacctgtg
      181 gcagttgtaa ataatgataa agaggcttcc tataatggta atactatggc aataggaaaa
      241 gacatggtgt ccaatttaaa agaaaataaa accttggatt ttcattttgt agatgaagag
      301 gaaggaaaga agggattgga agatggcgat tactatatgg tagtgacttt accaagtgat
      361 ttatctgaaa aaacaactac attatccaat attcaatcga cagcagctta tcaatcattg
      421 acaagtgagc aacaaactga gataagtgat tctgtatctc aaaattcaac tgatagtatt
      481 caatcggctc agtcaattgt agctttagta caagatttac agggaagttt agaaaactta
      541 caaaatcaat cttctaatct ttcgacttta aaaaatcaat ctaatcaagt atcacctatt
      601 acttctactt ctttgatagg attgtcaagt ggattaacag agatacaagg agatgttact
      661 agcaaattag ttcctgccag tcagtcgatt gcatcaggtg taaacgcata tactacaggt
      721 gttgataaag tttctcaggg cgcaagtcaa ctaagtgaaa aaaatgccac cttgacaggt
      781 agtttgaata aactagtttc aggctcaaac accttgacac aaaaatcttc tagattgaca
      841 gcaggagttg gttaattaca atcaggatct gggcaattag cagacaaatc cagtcagtta
      901 ctttcaggtg cttctccatt agagaataga gctaataaat tggcagatgg atctgggaaa
      961 ctagcagaag gtggaacaaa gttaacttct ggattggaag atttacagac aggacttgct
     1021 tctttaggac aaggactagg taatgctagt gatcaactca aatcagtatc aacagaatct
     1081 aaaaatgcag agattttgtc aaatccactc aatctttcaa aaacagacaa tgatcaagtt
     1141 cctgtaaatg gaatcgcaat agctccttat atgatatcag ttgctctttt ttttgcagca
     1201 atatcaacaa atatgatatt tgcgaaattg ccttcaggac gtcatccaga gagccgttgg
     1261 gcttggttga aatcttgagc tgaaataaat ggtattatag ctgttttggc aggaattttg
     1321 gtatatggag gagttcagct tattggttta actgctaatc atgagatgag aatatttatt
     1381 ctcatcatcc taacaagttt agtattcatg tctatggtga ccactttagc aacgtggaat
     1441 agccgtatag gagctttttt ctcacttatt ttgcttttac tacagttagc atcaagtgca
     1501 ggtacttatc cacttgcttt gacaaatgat ttctttagat ctattaatcc ctggttacca
     1561 atgagctatt cagtttcggg attacgacaa acaatctcta tcaacaagtc attttcctag
     1621 ctgtcatact agctctattt actggtttag gtatgctagc ctatcaacat aagaaaatgg
     1681 aagaagatta aaaaaatcga ccgattaact ggtcgatttt ttatgcctta gatgactttc
     1741 gtctgtgatt atagattcca aatagtaaga gagaagtaaa ggaacagatt gctccagtaa
     1801 taaaaccatt gggaatgaag gaaagtgtaa tagttccttt ccccttgaga atgtcaactt
     1861 tcataaatcc agtttgagct tgtttaattt ctattttctt accatcttgg taggcagacc
     1921 aacctttgtc ataaggaatg gtgaagaaaa tagatgtatc ttgttggaca tcatatgtag
     1981 caaaaacctt gtttttagaa gttgatactg tgacaggttg ttctttaatt ttttgaattg
     2041 cctcggtgaa agttttggta tctaaacgat agaaggtagg agattcaaat gatacttgtg
     2101 aatttccagg gaaactaaca ttgatattga aagttttttt ctctttagta tatcctagat
     2161 taaagaagga gaagacatta tcagttgtaa aagtcttttt ttcaccattt acaaggatgt
     2221 caaccttctt ttgtttatcg ttagaaaagt gaaggtttat gaaagagaga taaacttggc
     2281 tgttttctgg aacttcaatt tgatactgga ttgctgcatc ttcatttgaa gaacttgtga
     2341 cactaatcaa atcattagta ttttctattt tttctgtttt ttcataaggt attggagaaa
     2401 aataatcaaa attgacgtta gcaagttgat ttaaaaatga ggcctgatta tccaaggtat
     2461 gttcattgaa cttgacatca ttgtaaacag attgactagc aactgcaatc ggaagagagt
     2521 attgattttc atatagggta agattatctt tttgatagat atctttaaag ccatacttat
     2581 caataggact gtctgagata ttgtactgga taccaaataa actatcagcc aaaatactat
     2641 tatttgcata tcggagattg agattagtcc cagaggattt aaaaccaagt ttatctaaag
     2701 tagagcttga tgaacgattt cgaacagatg aaaattgaga gattccattg tagttgaatt
     2761 tcatactgtc atttcctgtc tgagtttgta gtttttcagt acgagtaaat tgatttccaa
     2821 tatatgttga gaaagattcc atagctggga tatctcgact ataagcactt cgagaagcaa
     2881 atccccattc cttagcaatt ccgtccattt gagatgaagc atttaaactc atttcaacca
     2941 gtataaataa agagattaga atggcaaata gattcacaga tataaacttt ttgataactg
     3001 caaggagtaa aagagaatag acaaccaaaa attcaagagt aagcagaata ttcaaatctg
     3061 ttaaaaaaga ataatgcgat tttagataga tggtagctaa aaatcctgtt actacaagaa
     3121 aaagcgaaac taaaaaattc cagactttaa gttctttcag acgctttaag acttctgctg
     3181 ctgtgtaaat taacaaggta gagaaaatcc aagcatagcg atgtaaaaac atgtttggag
     3241 tatgcatgcc ttgccaaaat aagtcaagag cttctatgta aaagcttgca attagaaatg
     3301 caaagaatat tatatatatg agtttcacgt gaaacttaat agatttcagc gtaaaaaata
     3361 aaatggtcaa aataaaggga agtagtccaa caaaaatcat tgggatggcc ccatactttg
     3421 ttgtgtcaaa ggaaccaatg aattgcttag caaagagatc aagataccag ctactttcag
     3481 tttgaaactt tgtaacttca gtcaattttt ccccatgtgt ctgtaaatca aacagagtgg
     3541 gaagagtcat aatcaaacta gccataccag ctaaaaagga gataactatg aaatcaagaa
     3601 cagatgattt tcgagtctta aagtcccacg aaatttgaca gagataccag aaaataagaa
     3661 acaatactgt catatatcca aaataataat tttgaataaa taagattgac agacttgtaa
     3721 agtacaatag gagtttcttt tcagttatca gtagatgtaa accagttata attaaaggaa
     3781 tcaagataaa aacatctagc caggttttta tctctaattg actgacagtg aaactcatca
     3841 gagcatagga agtagataag gctagcttta aagtctgagg gatagattga aacaatttat
     3901 tcaaactaaa aaaggttgac agaccaatca atccaaattt taagagagtg gtcagataga
     3961 cagcatctgg catattcgtt agatcaaaaa agtaaactag aggcgcgaga aaactaccca
     4021 agtaataact agatagggca tagaagttta gccctagacc acttgtaaag gtgtaaaaca
     4081 gactactatt tccatgtagg atatttcgta aggctacatc aaaaataacg tattgatgaa
     4141 agccatctcc taatagagga gagtcgtcgc tattccagta gataccttga gatagatata
     4201 ctccagacat aatcactaca ggaatgatga aagaaataaa ataggttcga tatgttttta
     4261 aaaatgattt catgttacct cgtagaatga tagaaaactc agttggttaa cccaactgag
     4321 ttttgaagtt ttatttagtc tttccaaagt tctttaactt ttgcttgtac ttctgcattt
     4381 tctaggaatt catcgtaggt ttcatcgata cggtcaatga cgccattttt agataagaca
     4441 atgatatggt tagccaaagt ttgaataaat tcgtggtcat ggctagcaaa gatgattgat
     4501 tctttaaagt ttttcaatcc atcattcaag cttgagatag attccaagtc caagtgattt
     4561 gttggatcat caagtacaag gacatttgat tttaagagca tgagttttga aagcatgaca
     4621 cgaacttttt ctccccctga caagacattt acaggtttgt taacttcatc tccagagaag
     4681 agcatacggc cgaggaaacc acgtaggaaa gtattgtcat cttcttcttt acttgcgaat
     4741 tgacgcaacc agtcaagaat tgattctcct cctgcaaaat cagctgagtt atcttttggt
     4801 aggtaagatt gactagttgt aactccccac ttgacagttc cttcatagtc aatatctccc
     4861 atgattgcac gaattaatgc agttgtttga atatcatttt gtccaataag tgctgtctta
     4921 tcatctggac gcaagatgaa actaatatta tccaagatag tttcaccatc aatctttaca
     4981 gttagatttt ctactgtcaa gagatcatta ccaatctcac gttccgcttt aaagttgata
     5041 aatggatatt tacgactaga tggtacaatc tcttctagct caatcttatc aagcattttc
     5101 ttacgtgatg ttgcctgcct tgacttagaa gcattggcag agaaacgagc aacgaattct
     5161 tgcaattgtt taattttttc ttctgcttta gcattacggt ctgctagcaa tttagcagca
     5221 agctcagaag attccttcca gaagtcgtag tttccgacat agagtttgat ttttccaaag
     5281 tcaaggtcgg ccatgtgagt acaaactttg tttaagaagt gacggtcgtg ggatactacg
     5341 ataactgtgt tatcaaagtc aatcaagaag tcttctaacc aagtaatcga ttggatatcc
     5401 aaaccgttag taggctcgtc caagagaaga acatctggtt taccaaaaag tgctttggcg
     5461 aggagaacct ttactttttc accgttggcc aattcgctca tgttttggta gtgtaattct
     5521 tctggaatgt ttaggttttg aagtagttga gaggcttcac tctctgcttc ccaacctcca
     5581 agttcggcaa attctccttc gagttcggca gcacgaaccc cgtcctcgtc tgagaaatct
     5641 tccttcatgt agatagcatc tttctctttc atgatgctat aaagtttttc atttcccatg
     5701 ataacgacat caatggcacg ttcatcttcg tagtcaaagt gattttgacg aagaacagag
     5761 agacgttcat ctggaccaag agagatgtga ccagtagtag gttcgatatc tccagctaaa
     5821 atttttaaaa aggttgattt tccggcacca ttagcaccga ttaatccgta agtatttcct
     5881 tctgtaaatt tgatattgac atcatcaaaa agtttgcgat cactaaaacg tagtgaaaca
     5941 tcagatactg taagcaatat ttt
//
LOCUS       DAVLSL010000115         5717 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLSL010000115 DAVLSL010000000
VERSION     DAVLSL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.117"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(62..295)
                     /gene="tnpB"
                     /locus_tag="VXN30_001645"
                     /pseudo
     CDS             complement(62..295)
                     /gene="tnpB"
                     /locus_tag="VXN30_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(276..461)
                     /locus_tag="VXN30_001646"
     CDS             complement(276..461)
                     /locus_tag="VXN30_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358243.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(765..2327)
                     /gene="guaA"
                     /locus_tag="VXN30_001647"
     CDS             complement(765..2327)
                     /gene="guaA"
                     /locus_tag="VXN30_001647"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1358244.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIRDIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2469..3167
                     /locus_tag="VXN30_001648"
     CDS             2469..3167
                     /locus_tag="VXN30_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1358245.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERHVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3210..4106)
                     /locus_tag="VXN30_001649"
     CDS             complement(3210..4106)
                     /locus_tag="VXN30_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1358246.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSAFTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(4115..5050)
                     /locus_tag="VXN30_001650"
     CDS             complement(4115..5050)
                     /locus_tag="VXN30_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1358247.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGSVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5047..>5717)
                     /locus_tag="VXN30_001651"
     CDS             complement(5047..>5717)
                     /locus_tag="VXN30_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteinase"
                     /protein_id="HEV1358248.1"
                     /translation="LNETFYIETLGMKALLEESAFLSLGDQTGLEKLVLEEAPSMRTR
                     KVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEIFSPEDDLILIHAE
                     DDRASLVEVGEKPEFQTDLVSISLSKFEISMELHLPTDIESFLESSEIGASLDFIPAQ
                     GQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYFIPKSEKFFLCKDRNNVELW
                     FEEV"
BASE COUNT         1783 a         1239 c         1058 g         1637 t
ORIGIN      
        1 tcataagcca gtctacttgt tcaggtgtga gagctttgac atccttttct gtactgggtc
       61 aagtcagtct gccgttctca aagcgtttat atagtagcca aaatccttga ccatcccagt
      121 aaagggcttt aaagcggtct ttacgtccac cacaaaagag aaaaacttga ccggagaaag
      181 gatctaattc aaagtgggtt ttaaccagat aagccagtga atcaatgcct tgcctcatat
      241 ccgttttccc acatacgaga tagacctgcc ctaggctaga tagatgaatt gtcatagagc
      301 aacaccttat ccaaaagctg ttctaccatt tcgagattga gagattgaaa aaaacttact
      361 tcaagcttgc caatacgaat tttaagcaga atatcatttc tctttttaga atcaaaagga
      421 cgagattgtg gaatctctac aggaacaata ggttgtggca ttaaaatatc ctccaatagt
      481 tttactttac taatagtata caggaggagc tccatgaatg atagatacct tgttatgacg
      541 cgcttaccat attagaaagt tggctctcta attaaattaa ttctcattat atatatcaaa
      601 agaccgcagt ttgaaaacta cggtctaatt ttttgtccaa atgtaaacca cttggaacta
      661 ttattcaatt tattcttttt agcaaatgtt cccgttttaa tcccagttac agtttttaat
      721 cctttgtaac cgttgatttt acaaagcttt taattttttt gcgattactc ccactcaact
      781 gttgcaggtg gtttacttgt aatatcgtag acgatacggt taacatgatc cacttcattt
      841 acgatacgta ctgagatttt ttgaagtact tcccatggaa ttttggcaaa atcagcagtc
      901 ataccatcga tagaagtgat agcacggatt gcaatcgtgt agtcatacgt acgaccgtca
      961 cccataacac cgactgaacg aacgcctgtg ttaacagtga agtattgcca aatatcgcgg
     1021 tcaagtccag ctttagcgat ttcttcacga agaatagcgt ctgattcacg aacggtttca
     1081 agtttctctt cagtgatttc acccatgaca cggatagcaa gtcctggtcc tgggaatggt
     1141 tggcgccata cgatatggtc tggcatacca agctctgtac caagagcacg aacttcatcc
     1201 ttgtaaagag tattgagtgg ttcaatcaat tcaaactgca tatcttctgg aagaccaccc
     1261 acgttgtggt gtgacttgat agtttgagct gtatccgtac cagactcgat aacatctgta
     1321 tataaagtac cttgagcaag gaatttcaca tctttgagct tgcttgcttc gtcatcgaat
     1381 acatagacaa actcgttacc gatgatttta cgtttttgtt caggatcaga aacgccagca
     1441 agtttgtcaa ggaaacgttt agcagcgtct gctttgacga tattcaaacc aaacttacca
     1501 ccgagcatgt ccataacttg atcagcttcg cctttacgaa gaagaccgtg gtccacgaag
     1561 atacagatca attgatcgcc aatcgctttt tggagaagaa ccccaacgac agatgagtca
     1621 acaccacctg atagaccaag aaggacacgt ttatcaccga cggtttcacg aatttttttg
     1681 atctgcatgt caatgaaatt atccattgac cagtcacctt tagccttaca aatgttaagg
     1741 gcaaagttac gaaggatatc atttccgtat acagaatgac gaacttctgg gtggaattgg
     1801 ataccgtaaa tgtgtttatc tgggttttcg atggctgcgt atgggcagtc agctgatgta
     1861 cctgtacgaa caaagtcagc aggaatctca gtaaccgcat caccatggct catcaaaaca
     1921 gtctgttcat caggtgttga ttcaaaaagc gctgatggtg tgtgagttag ggttgattga
     1981 ccgtattcac gatttccagc atcacctgca ggaacaactt ttcctccaag tttatgggtc
     2041 aataactgca taccataaca gattcccaaa attggaattc cgagttcgaa gatttctggg
     2101 tcaatatcaa atgaaccatc ttcatataca gaatttggac cacctgatag aataattcct
     2161 acaggattga cttcacgaac ttcagcagct gaaattttat ggctttttag ttctgaaaaa
     2221 acaccaatat cacggatacg gcgtgaaatc agctggttgt actggctacc atagtccaat
     2281 acgatgattt tttctacatc ttgcaaatca gttgaaatgt tgctcatctt tttcctttct
     2341 caaaagaaat atcttttctc aatattctat cacaaataag ggcgaattac caactaaaca
     2401 aggcgaagtt tgactttttc tagtttattg gggtacaata gagaaaagat agaaatgaag
     2461 tgaaaaatat gctacctgct tatatgaaaa tccatgatca gataaaaaag gatattgacg
     2521 agcaccgttg ggctattggt gagaggcttc ccagtgaaag agatttagct gagcagtttg
     2581 cggtcagtcg catgaccctc cgccaagccg tatctctatt agtcgaagaa ggcgtcttag
     2641 agcgccatgt aggaagcggc acctttgttt ccagtactcg agtacaagaa aagatgcgag
     2701 ggacaaccag ttttactgaa attgtcaaat cccaaggtaa agttccctct agccagctca
     2761 tttcctacaa aaaaaccatt cccaatgagc aagaagttgc caagctagga atttttccaa
     2821 cggacaatat tatccgaatg gagcgggtcc gctatgccga ccaagttccc ctagtttatg
     2881 aagttgcttc tattcctgaa aaattcatta aggactttaa aaaagaagaa atcaccagtc
     2941 atttcttcca aaccttgcaa aaacatggct atcgtatcgg caaatctcaa cagaccatct
     3001 atgctcgcct tgctaaagaa aagattgccc actatttgga agttgaaaaa ggacatgcta
     3061 ttcttggatt gacccaggtt tcctacctag aagatggtac tgcttttgaa tacgtaaaaa
     3121 gtcaatatgt aggcgaacgc tttgaatttt atcttgaaaa taattagatt accacgcaaa
     3181 aactctctgt cacggacaaa gagtcttttt tatttttcta agaataaatc tttcaaagaa
     3241 atcaaagtta cagcaaacaa tatcattgcc ataccaagaa aatcaatggg ataaaattgt
     3301 tcattcatta ttaagaaggc aaagaaaatc gccgatattg gctcaattga agccaacaag
     3361 cttgacttga ccggtcctat cagactggct cctttaagga aagctgtata ggcaaagaca
     3421 gtcccgataa ggataatgcc tgcaaacgca aggagaaaat caagactagt cgggatatcg
     3481 gcctgtagaa cccctgtaaa aggaagagcg accaaacctg ctatgaccat tcccacacca
     3541 atgaccaagc tgctccccca ctttttaatc aaggctatgg gtaaaatgat atacagagca
     3601 taagtaaagg cagaaaagag accccagaac agaccagcag gtgtcatgga taactggtcc
     3661 aactgcccat gtgttgcgat caggaaggtt cctccgatgg cgaatatgat ggaaactatc
     3721 tctcccagtg tcggtgccac cctatccttg atacagctat aaattaaaat tccgacagga
     3781 caaacatact gaagcaccgt cgctgttccc gcattggtct cctgaatagc agacagatag
     3841 gcgaattggt tgagaaaaag accaatcaga gcaaaaataa gaagagacag caaactcttt
     3901 ctatccttta aaaagaccag tattttatcc tttgcagtag cataagccaa gagcatgaga
     3961 attccaccag cgattaaaag acgcaagttg gtcaagacca gagccgaaat tccgtgtgcc
     4021 attaggtatt ggccactcgt tcctgacaac ccccaagcaa taccagccac aactgttagt
     4081 aaagtccctt ttaaactatt tgacatgtct ctccttactc ttctttgcga atcaagttca
     4141 tcacttgatt gcggtctaat atccattgag ctctctcgtc cttttcatag gtacgattcg
     4201 ataggaaaat agtggcttct tgcttctgac gattccacat gataaaggta cctgtatagc
     4261 ccgtatggtc tagccaatct ccttccaaat tccatgctaa agaacgttcc ttgtcatcca
     4321 aaggagaaaa attttgactt aagtctcttg caaaatcatc tgctaaatag tgttctaaaa
     4381 agatttgtaa atcctttata gtcgaaaata aaccagcact cccagcatgt ctacccagga
     4441 gacgagcctt gggatcatgc actatgcctg cctctacacc tctaactgtt ggaacagcaa
     4501 gctcaactga cccaaacttg gtttccgtca ttccccaagg tttccagact tgatccttta
     4561 aaatcacatc caaatcttga ttaaaaattc tttccaaaat aaagcccaac agcaaaaaat
     4621 ggacatccga ataaagaaag gctggctgac ttcgtctgtt gagatgaaac atcgcttcct
     4681 ttaattcagg ggctgttaaa agatcacgat taggaataaa aggatcaagg tctgttgcat
     4741 gagtcaagag ctggcgaata gtgatgtctg gataatcact ctcaggtaaa aaatctatta
     4801 ccagtctatc aatatctaat tgacctattt cccacaagaa ggtacaaact gtgccaaccc
     4861 caacaacctt gctgacacta gctaggtcat aaactagtcc tgcctcagtc tgcaagccat
     4921 gctctgggtc actctggcct aaatagaact ctgtccattg attgtcctta aaatacgcaa
     4981 aagaggctcc gggataaatt cctgcctcga tttgttcttc tattttttta ataatcttgg
     5041 tccacttcat acttcttcaa accacaattc aacattattt ctatctttac aaaggaagaa
     5101 tttttcagac ttaggaataa aatattcagt agactcaaac ttctggcgaa gacttgctat
     5161 gtctaattca ttgaccaaga acttgagcat agataagtcc caggtaaccg tattgtccac
     5221 agtcaaatcc tgcccctgag ctggaataaa atcaagggat gccccaattt cagatgattc
     5281 caagaaactt tcgatatcag ttgggagatg taattccata gaaatctcaa atttacttaa
     5341 agaaattgat accaaatctg tttgaaattc aggcttttct cctacttcta ctagacttgc
     5401 tctgtcatct tccgcatgaa tcaaaatcaa atcatcttct ggtgagaaaa tttcaaaagc
     5461 gtagccattt tgacctttat ataatcgatg aatcgaatct gttttagata agattccttc
     5521 aatttctaag ggattttcca ccttgacaat caatctagct agtttttttc ttccctctac
     5581 cttacgagta cgcatactgg gagcttcttc taaaaccagc ttttcaagac ccgtttggtc
     5641 acctagtgac agaaaggccg attcttctaa caaggccttc attccaaggg tttcaatata
     5701 aaatgtttca tttaatt
//
LOCUS       DAVLSL010000116         5699 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLSL010000116 DAVLSL010000000
VERSION     DAVLSL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.118"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            109..504
                     /locus_tag="VXN30_001652"
     CDS             109..504
                     /locus_tag="VXN30_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1358249.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            515..886
                     /locus_tag="VXN30_001653"
     CDS             515..886
                     /locus_tag="VXN30_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1358250.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            896..3139
                     /locus_tag="VXN30_001654"
     CDS             896..3139
                     /locus_tag="VXN30_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1358251.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3346..5121
                     /gene="spxB"
                     /locus_tag="VXN30_001655"
     CDS             3346..5121
                     /gene="spxB"
                     /locus_tag="VXN30_001655"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1358252.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5232..5579
                     /locus_tag="VXN30_001656"
     CDS             5232..5579
                     /locus_tag="VXN30_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1358253.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT         1692 a         1060 c         1312 g         1635 t
ORIGIN      
        1 taaaattcta tttttcgaag aaaatattat ttctataatt gacaaatgta gattttggga
       61 gtatactgtt gagtgtaatt gacaaatgta gattttggag gtatggtaat gcagatttca
      121 gatgcagaat ggcaggtcat gaagattatt tggatgcagg gggagcagac cagtacagat
      181 ttgattaggg ttttggcaga gcggtttgac tggtccaagt ccacggttca gacacttttg
      241 gctcgtctag ttgagaaaga gtgtttgact cggaaaaaag aaggcaagtc ctttgtttat
      301 tcagcgcttt taactctaga ccaaagtcgg gatttacttg tccaagatat caaagacaag
      361 gtttgttccc gtaggattag gaatttgttg gctgacttga ttgtagaatg tgaatttact
      421 cagactgact tggaagactt ggaagctgtg atttcagaga agaaatcaag tgctgtaaca
      481 gaagtaagat gtaattgtat gtaaaggaga cgtcatgtta aatagtattg taaccattat
      541 ttgtattgcc cttatcgcgt ttatcttgtt ttggtttttc aaaaagcctg aaaaatctgg
      601 acaaaaagcc cagcaaaaaa acggatacca agagattcga gtggaagtca tgggaggcta
      661 tactcctgag ttgattgtcc tcaagaaatc agtgccagcc cgcattgtct ttgaccgcaa
      721 ggatccttca ccatgtctgg atcaaattgt ttttccagat tttggtgtac atgcgaacct
      781 gccaatgggg gaagagtatg tagtggaaat cacgcctgaa caggctggag agtttagctt
      841 tgcttgtggt atgaacatga tgcacggcaa gatgattgta gagtaggtgg agactatgac
      901 agaaattgtg aaagcaagct tagaaaatgg cattcaaaaa atccgtatcc aagctgaaaa
      961 aggctatcat ccagcccata tccagcttca aaagggaatt ccagctgaga ttacctttca
     1021 tcgtgctact ccttcaaact gttataagga aattctgttt gaagaagaag gtatcttgga
     1081 accaatcggc gtagatgagg agaaagtcat tcgttttaca cctcaagaat tagggcgaca
     1141 tgaattttct tgtggcatga agatgcaaaa gggaagctat acagtcgttg agaagactcg
     1201 aaaatctcta tctctcctgc aacgtttttg gattactagt atctgtactg tgcctcttgt
     1261 gattctcatg attgggatgt tggcaggtag cattagtcat caagtcatgc attggggaac
     1321 ctttctagca acaacgccta ttatgttagt tgcaggtaag ccatatatcc agagtgcttg
     1381 ggccagtttt aaaaagcaca atgccaacat ggataccttg gttgcgctgg gaactctagt
     1441 ggcttatttc tatagcctag ttgctctctt tgctggtctc cctgtttact tcgaaagtgc
     1501 tggatttatc ctctttttcg ttcttttggg agcagttttt gaggaaaaaa tgagaaaaaa
     1561 tacgtcccaa gctgtggaga aattactgga cttgcaagct aaaaccgcag aagtcttgag
     1621 tgatgatagt tatgtccaag ttcctttgga acaagtcaag gtaggcgacc tgattcgagt
     1681 gcgtcccggt gaaaagattg ctgttgatgg tgtcgtagta gaaggtgtct ctagtattga
     1741 cgaatccatg gtgacaggtg agagtctgcc tgtggacaag acagttggag atactgtcat
     1801 tggctcaacc atcaatcata gtggaacgct tgtctttaga gcagaaaaag ttggctcaga
     1861 gactgttttg gctcagattg tggattttgt gaagaaagct cagacaagtc gtgcgccgat
     1921 tcaggacttg acggataaga tttcagggat ttttgtccca gtagttgtca ttttaggaat
     1981 catgaccttt tgggtttggt tcgtcttgct cagggatagt gtggttgtgc ttggagctag
     2041 ctttgtgtcc tctcttctct acggagtggc ggttttgatt atcgcctgtc cttgtgcctt
     2101 gggacttgca acaccgacag cccttatggt ggggacagga cgtagtgcca agatgggggt
     2161 tctcctcaaa aatggaactg tcttacagga aatccagaaa gttcaaaccc ttgtctttga
     2221 taagaccggg actttgacgg aagggaaacc tgtggtaaca gatgtcatcg gcgacgaagt
     2281 agaagtgttt ggattggcag cctccttgga agatgcttct caacacccac tggctgaggc
     2341 tatcgttaag cgagcgagtg aagctggact tgagtttcaa actgttgaaa attttcaggc
     2401 cttgcacggg aaaggtgttt cagggcgaat caatggaaaa caagttttac ttggaaatgc
     2461 taaaatgctg gatggcatgg atatttctaa tacttatcaa gataaactag aagaactaga
     2521 aaaagaagct aagacagttg tgttcttagc tgttgacaat gaaatcaaag gcttgcttgc
     2581 tttgcaagat attcctaaga aaaatgctaa gctagccatc agtcagctaa aaaaacgtgg
     2641 tctcgggaca gtcatgctga caggagacaa tgctggtgtg gcgcgtgcta ttgcagatca
     2701 aatcggaatt gaagaggtca ttgcaggcgt cttgccagaa gaaaaagccc atgaaatcca
     2761 taaactgcaa cagtcaggta aagtagcctt tgttggtgat gggatcaatg acgctcctgc
     2821 ccttagtgta gcagatgtgg ggattgctat gggagctgga acagatatcg ccatcgagtc
     2881 agcagatttg gtgttgacaa ccaataatct tttaggagtg gtgcgtgcct tcgacatgag
     2941 taagaaaacc tttcatcgta ttctgctcaa tcttttctgg gcttttatct acaatgttgt
     3001 cggaattccg attgcagcag gagtcttttc aggtgttgga ctggctctca acccagaatt
     3061 ggcaggtcta gccatggcct ttagttctgt atctgttctg accagttccc ttctgttaaa
     3121 ttttagtaaa atagactaaa agtaatgact agattttttt gttataaaac agaaatgacg
     3181 acactttcaa aactgtatta gaatagagaa tagagagttt tgagcagatt tttagaaaag
     3241 tcagcataaa tatgatacag tggaatagta aaaatttgga gaacgtttcc aattctatgt
     3301 aatcgtattc tccaagtcta aaaaaattga aggagagtta tcattatgac tcaagggaaa
     3361 attactgcat ctgcagcaat gcttaacgta ttgaaaacat ggggcgtaga tacaatctac
     3421 ggtatcccat caggaacact cagctcattg atggacgctt tggctgaaga caaagatatc
     3481 cgcttcttac aagttcgcca cgaagagaca ggtgctcttg cagcggttat gcaagctaaa
     3541 ttcggcggct caatcggggt tgcagttggt tcaggtggtc caggtgcgac tcacttgatt
     3601 aacggtgttt acgatgcagc tatggataac actccattcc tagcaatcct tggatcacgt
     3661 ccagttaacg aattgaacat ggatgctttc caagagctta accaaaaccc aatgtacaac
     3721 ggtatcgctg tttacaacaa acgtgtagct tacgctgagc aattgccaaa agtaattgac
     3781 gaagcctgcc gtgctgcagt ttctaaaaaa ggtccagctg ttgttgaaat tccagtaaac
     3841 ttcggtttcc aagaaatcga cgaaaactca tactacggtt caggttcata cgaacgctca
     3901 ttcatcgctc ctgctttgaa cgaagttgaa atcgacaaag ctgttgaaat cttgaacaat
     3961 gctgaacgcc cagttatcta tgctggattt ggtggtgtta aagctggtga agtgattact
     4021 gaattgtcac gtaaaatcaa agcaccaatc atcacaactg gtaaaaactt tgaagctttc
     4081 gaatggaact atgaaggttt gacaggttct gcttatcgtg ttggttggaa accagccaac
     4141 gaagtggtct ttgaagcaga cacagttctt ttccttggtt caaacttccc atttgctgaa
     4201 gtttacgaag cattcaagaa cactgaaaaa ttcatccaag ttgatatcga cccttacaaa
     4261 cttggtaaac gtcatgccct tgacgcttca atccttggtg atgctggtca agcagctaaa
     4321 gctatccttg acaaagtaaa cccagttgaa tcaactccat ggtggcgtgc aaacgttaag
     4381 aacaaccaaa actggcgtga ttacatgaac aaactcgaag gtaaaactga gggtgaattg
     4441 caattgtatc aagtttacaa tgcaatcaac aaacatgctg atcaagacgc tatctactca
     4501 atcgacgtag gtaacactac tcaaacatct actcgtcacc ttcacatgac acctaagaac
     4561 atgtggcgta catctccact ctttgcgaca atgggtattg cccttcctgg tggtatcgct
     4621 gctaagaaag acaatccaga tcgccaagta tggaacatca tgggtgacgg agcattcaac
     4681 atgtgctacc cagacgttat cacaaacgtt caatacgacc ttccagttat caaccttgtc
     4741 ttctcaaatg ctgagtacgg cttcatcaag aacaaatacg aagatacaaa caaacacttg
     4801 tttggtgtag acttcacaaa cgctgactac gctaaaattg cggaagctca aggagctgtt
     4861 ggattcacag ttgaccgtat cgaagacatc gatgcagttg ttgcagaagc tgttaaattg
     4921 aacaaagaag gtaaaactgt tgtcatcgat gctcgcatca ctcaacaccg tccacttcca
     4981 gtagaagtac ttgaattgga tccaaaactt cactcagaag aagctatcaa agccttcaag
     5041 gaaaaatacg aagcagaaga actcgtacca ttccgtctct tcttggaaga agaaggattg
     5101 caatcacgcg caattaaata attcctctcg ccgaaaatca aatatgaaac ttgtagactt
     5161 tattctttga ttttcataga gcagaacttt aaaagatcgg agcaatccgg tctttttatg
     5221 gaggagagta aatgaattta aatcaattag atattatcgt ttcaaatgtt ccccaagtct
     5281 gtgctgactt ggagcatatt ttggataaaa aggcagatta tgctgatgat ggttttgctc
     5341 agtttacgat tggtagtcac tgccttatgt tgtcacaaaa tcatttggtt cctttggaaa
     5401 actttcagtc aggaatcatt attcatatcg aggttgagga tgtagaccag aactacaaac
     5461 ggttgaacga gcttggtata aaggttttac acggaccaac tgtaaccgat tggggaacag
     5521 agtccttatt agttcaagga cctgctggtc tagtgcttga tttttatcgt atgaaatagg
     5581 tcgcgtagtc atttgacgtt aaccttagct atttttaaaa tagaaattga gaatatgaac
     5641 tattgaaaga tcggagcaat ccggtctttt tatataaaga aattgcatat aatactcat
//
LOCUS       DAVLSL010000117         5654 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLSL010000117 DAVLSL010000000
VERSION     DAVLSL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.119"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            85..552
                     /locus_tag="VXN30_001657"
     CDS             85..552
                     /locus_tag="VXN30_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1358254.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            585..678
                     /gene="ffs"
                     /locus_tag="VXN30_001658"
     ncRNA           585..678
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VXN30_001658"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(761..1902)
                     /locus_tag="VXN30_001659"
                     /pseudo
     CDS             complement(761..1902)
                     /locus_tag="VXN30_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(1957..3027)
                     /locus_tag="VXN30_001660"
     CDS             complement(1957..3027)
                     /locus_tag="VXN30_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEV1358255.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            complement(3044..3424)
                     /locus_tag="VXN30_001661"
     CDS             complement(3044..3424)
                     /locus_tag="VXN30_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1358256.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            complement(3437..3700)
                     /locus_tag="VXN30_001662"
     CDS             complement(3437..3700)
                     /locus_tag="VXN30_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1358257.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(3700..3933)
                     /locus_tag="VXN30_001663"
     CDS             complement(3700..3933)
                     /locus_tag="VXN30_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358258.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(3933..4301)
                     /locus_tag="VXN30_001664"
     CDS             complement(3933..4301)
                     /locus_tag="VXN30_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358259.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            4874..5065
                     /locus_tag="VXN30_001665"
     CDS             4874..5065
                     /locus_tag="VXN30_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1358260.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            5088..5291
                     /locus_tag="VXN30_001666"
     CDS             5088..5291
                     /locus_tag="VXN30_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358261.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(5446..5613)
                     /locus_tag="VXN30_001667"
     CDS             complement(5446..5613)
                     /locus_tag="VXN30_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEV1358262.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
BASE COUNT         1591 a          918 c         1161 g         1984 t
ORIGIN      
        1 tagagtcacc gcattcggtg gcttcttttg tgttgggatt catgatataa taataaaatc
       61 gataagtagg aaaagagaaa agagatgtat tatacgcttg aagaaaaaga agtctttatg
      121 agggaggctt tgagagaggc tgagattgct cttgaacacg atgaaattcc aattggttgt
      181 gtgattgtca aagatgggga aatcattggt cgtgggcata atgcgcgtga ggaattacag
      241 cgagcggtta tgcatgcgga aattatggct atagaggatg cgaacttgag tgaggagagc
      301 tggcgcttgc tggattgcac actttttgtg accattgaac cttgtgtcat gtgtagtggg
      361 gcgattgggc ttgcccgcat tccaaatgtg gtctatgggg ctaaaaacca gaaatttggc
      421 gctgctggga gtttgtacga tatcttgaca gatgagcgtc tcaatcatcg tgtggaggtt
      481 gaaataggaa ttttggaaga tgaatgcgca gctatcatgc aggatttttt tagaaataga
      541 cggaaaaaat aattttgctt ttaaaatgaa taggaatgtg atataataaa tagtggagca
      601 acagttctgc gtgaagcggg tcaggggagg aatccagcag ccctaagcga tttgaattgt
      661 gtgctctttt ttcgtgcact ttaaaaaccc tttaaaatca acactttaag gggtttttgt
      721 ttgtcttgta taagaaaaag gggcagacga ggggcacaat ttaaaatttt atcttgtcta
      781 acttgctaga tatgtctgat accatttttt gagtaacgtg agaataaatc tctagtgtgg
      841 tctttgagtc actatgccct actctgtcca tgatggcagt caagggaatg cccagctcag
      901 caagtaggga tatgtgagaa tgtctaaatg tatgtgtagt tatgtttttt tctatgccga
      961 ttttttgacc atgtcttttc aatgcacaaa taaccctggc atttgttatt ggttctccta
     1021 gagtattgat gaaaataaaa tctgtatcaa atccatttgt cgcattctct attatttgct
     1081 ctttgataat atctaacact ttttgaggtg ctgttataac cctatcggac ttgattgtct
     1141 ttggtgtagt tctctctttt tgtctgaaat cgtatgtatg cttgatgtga atagtctttt
     1201 tagaaaaatc tatatcttcc ttgtagttta aggctgccag ctctccatac ctcatgccag
     1261 taagaaaaag aactttagct attcggatat actttgtaat tcgataatca catagggcct
     1321 cgtcttttaa attttggatg aataacttaa actctttttg gtctaagtat tttgtatttt
     1381 tctttctgag ttcgtcggat gtaattactt ttctaggcgt ttcaacaaat agcatttcat
     1441 ttgtatcaat ataattcatt ctgatagcaa atttcattat ctgattgagc ttgaatttga
     1501 ttttagaaac atagttatga gatctcccat cttgtaatag ttgatctatt actttttgta
     1561 ataaacgtct atcaaatttc taactaggta gtcgccctct atctgcttta aaatctcttt
     1621 ttttacattc tttgaagcgt agacggttga gttttttacg ccgtgtttcc aattctcctc
     1681 aaattcttca tatagttttt caaaagttat attagaaaca gaatgttgtt tttctcctaa
     1741 cttttgtttt atcttttcct gaagtaaaat cgcagcttga tttcttgctt ggggagtttt
     1801 tttctccatc gttactgaga ctttttttaa tttctcagta tatggatctt tgtatctctc
     1861 aaaaaattta tatttgccgt ttggcaattc ttccatccac attgatttta cctcactttt
     1921 ttgataaaat gggtatagta aagagggctt tttttattat gagaatttct tgattacttc
     1981 taaaatttca ttggcataat ttgcgacttc gagaggggta gacaccggga agatgccttt
     2041 atcacggatt tcgatagtgc ttttttttga atttgaacga taacgtaata cccatttttt
     2101 gatgttatcg tcaaccaaaa cattgaaata gctacggtta tctctgtaaa atacacgttc
     2161 tggagaaact acatccctag caagcatttt aacaacagta taaacttcta attcagcagg
     2221 agttgtgata atttcgtcag ttacttcgac aatctcttca gcttcagctt caacttttgg
     2281 aatatcggtt gttactttgg tttcaacgct tgtattaagt gcagcactta atttttcatt
     2341 aactcgctct gtgatgaatt gattaaaccc tttcacaata atgggagaaa atgtcgttaa
     2401 gatattttga gtaacacgcc cttcatagat ttcagaagtt agatatctga ggaagctatc
     2461 tgacggtgta gtgatatttt cagtaaggaa cgctttaaga ttgttgaggt atttcaattc
     2521 agaagctgat gaaacaatat tatcaatatc aaaattctct ttgtggaatt tgatgatttc
     2581 agtaaattga ttttctttga tatcagtcac atcgattgtt aagaatggag ttgtgtccat
     2641 tttatttggc tcatctaaat cagtaaagaa tttatattct ctaccatttg tcaagatacc
     2701 gaattttgat ttagtagtta cgaaatatct gaatagttga gagtcgtgct tagtaaggtt
     2761 ttctgtaatt gatttacatt caattaggat ttgaggctcg ccatccaaaa tgatcgcata
     2821 gtcgactttt tcgccttttt taatgcccac gtcggcagta aattccggga caaattcaag
     2881 tggattgaag atatcatatc caagcgcttg gaagaacggc ataatgaagg cattctttgt
     2941 ttgttcttca ttcgtaatgc tttggctgag gtctgctaca cgtttgccga cttgttttaa
     3001 atcggctttt actttatcaa tttccatatt gatactcctt tttttaattt actaatgcta
     3061 aatactcttc tttaaccatg atttcatttg tcatggtttt tagattgtag taagacatga
     3121 atttgaggta atcaaactct gtggggtcgt ctaagttttc tagagcgtct tttacgagat
     3181 gatggatcat attcctatca gcttcgtttt cacagcgtag acgggcgttc tggtgctctg
     3241 agcgtgtgtg gtctttgtgt ccaagttcgt gtagggcgac ttggatttgt tgttcaggag
     3301 tcaagttgtg gtcaatagcg agcacgtttg tgtctggatt gtagaaaccg ctattgtgcc
     3361 agtttgaacc gtcgaagaga caaagttcta cttgatattc ttcgcagagt ttagcgagtg
     3421 tcatagttct ccttcgttat ttatagacat ctctgcggtg ggcgatttcc acggctagga
     3481 cgactagttt atcgtcttgg atatcacaga tgatgcggta attctctact ctgtatcgcc
     3541 agtaacctgc aaggttggct tttagtgctt ttccgtgttg tcgtggattg gtcgtgtttt
     3601 caatgttttt agcaagccag gataggattt gttttctggt tggggtatct agttttttaa
     3661 gttgcttgag agctttttta tcaatatcta gccgatacat taagcaatat cctctcgagt
     3721 tagtcctagt tcatccaaga cctcatccat ggtataagta gctgggtcgg ctaaatactc
     3781 catataggct tggtcggctg ctcgtgcgtc ttcgatatct tccatgagtg ccatgaagtc
     3841 gtcaaaatcc atggtcgttg tgtcgatacc gtgtttgttt aggtagtccg taatgtagga
     3901 atttttttct gtgaagttga tagtgatagt cattagcgtt ctcctttgct tttgaagtgg
     3961 gcggatagga cggatgtaat gaagtcgata tcatcttcat ttagtggttt cccgtcaaat
     4021 aacatggtgt tggctgctgc tttgcgtaag tctatgattt gtccgtttac ttgggcaaat
     4081 tcatcactcc cagcgatagc agggttatct gttcgtccga gcaggtagtc ggtggatacg
     4141 ttgaaataat tagcaatttc tgcgatacgt tcagcgtttg gcgtagaatt ttttatctta
     4201 tagagtgtat ttctaccata acctaagtct tcttctactt gtccaagagc ttttccacgc
     4261 ttttttgcca attcttttat tttttcaaat gtctcaaaca ttgttaaatc aacctttcta
     4321 agacatcaca aaaaaaaatt taacaaattt ggtgtaaaaa ggttgactaa ttatcccaaa
     4381 aggtgtaaaa tgttttttgt aagtgataaa caactaaaaa aacaactaag aaataaatta
     4441 taaaaatgtt ttggcgaacg gtatttatag atttattggt gtttttatta tgctttaatt
     4501 ttagccgatt tggtgtaagt tgtcaagtat aatgcagaaa aatagttaca tttttagttg
     4561 tttcttattt acaaataagt aaagaggagg aacgtgctga tggtaagtat tcttaaaaat
     4621 ttagaacaag aaaaagacca ccttgaaaaa gtcattaagg tagtcagcgc tggtggtaaa
     4681 tttctgagat tgccatatca aaaaagtcac gctcgattag tgagaatctg aaattgattt
     4741 ctcaaaatct ttaccatcat cgtagatgtc gcgtttgctt gctttgagta aatattcttt
     4801 aaaagtcata aggttaactc ctttctgctt atattatagc agaaaaagaa gaaagaaaaa
     4861 gaaaggagaa aatatgccag atatcgcaaa cggtcgcgaa agagttattg ctttcttgaa
     4921 agagaaaggc attaaaaaag caactctagc ggttgcttac ggctttaaac gacaggaagt
     4981 gacaaacatt ctaagtggaa cgactaaagg tccacgagcg aacagtttca ttcttcaggt
     5041 tattgaagat tatgggattg agtaggaaag atttgaggag taggaaaatg agaccaaaac
     5101 gatatccgta tagtggacaa aaagagtcca cctttgtaaa ggcagaccct gagttagttg
     5161 aaaaactttt aagaaacact agttttcttg agtgtttaca aaaaaagcct atcaattttc
     5221 agatagactc agaagaattt aagcgtctta gctatgaagc cattcatgat acttctcaag
     5281 taactcaata ggaggaagga ataaaatgat tcaccattat ataactcact atgccagcaa
     5341 tgggaaagat tacgccgaag catggattca aattgatttt ttgggaatgt gcttttgtgt
     5401 atggaaaaag cgtacaacca ttgaacgatt gtacgcaaac gaagattaga cttttttcca
     5461 accgttgcct ttagcagatg tcggagggag ccgatcacct tttccgatag ttgcggtatg
     5521 accattagta acttttccgc cacgaggtcc tacctctaca tagcgaccag gtttctgatt
     5581 atctgttcca ggttttattg gagtatttgc catactatct cctcctttct ataagtttga
     5641 gaaaatcaac ggta
//
LOCUS       DAVLSL010000118         5618 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLSL010000118 DAVLSL010000000
VERSION     DAVLSL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.120"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(101..1000)
                     /locus_tag="VXN30_001668"
     CDS             complement(101..1000)
                     /locus_tag="VXN30_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358263.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(1072..1632)
                     /gene="rnmV"
                     /locus_tag="VXN30_001669"
     CDS             complement(1072..1632)
                     /gene="rnmV"
                     /locus_tag="VXN30_001669"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1358264.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1632..2405)
                     /locus_tag="VXN30_001670"
     CDS             complement(1632..2405)
                     /locus_tag="VXN30_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1358265.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMIISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2710..3375)
                     /locus_tag="VXN30_001671"
     CDS             complement(2710..3375)
                     /locus_tag="VXN30_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1358266.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKLETTPGGDKGGNTGSSD
                     ANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKNKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRLVAPNAA
                     PKTTSAPQATAGTMQNVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTR
                     RRK"
     gene            complement(3638..3772)
                     /gene="rpmH"
                     /locus_tag="VXN30_001672"
     CDS             complement(3638..3772)
                     /gene="rpmH"
                     /locus_tag="VXN30_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1358267.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(3917..5131)
                     /locus_tag="VXN30_001673"
     CDS             complement(3917..5131)
                     /locus_tag="VXN30_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1358268.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5287..5580)
                     /locus_tag="VXN30_001674"
     CDS             complement(5287..5580)
                     /locus_tag="VXN30_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358269.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVVKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
BASE COUNT         1598 a         1298 c          989 g         1733 t
ORIGIN      
        1 tattattttt agcttatatc taaaaaaaca gttctaaaga cgaactttag aactgttttg
       61 atacgaggac aaggacattt ttgtcactga ttcactatgt ttataaatat tttttgacat
      121 cctcttgcca ttcctctgtc aatttcttta ctagaaatcc atctccaatt agcttcgcaa
      181 atgttttaga agactgataa aatgattcag ctaaactaaa atctctttct tctcttaatg
      241 cttgcttcca caaaaacata aatacaagag gtttcttttg ataatcttga gttttatcca
      301 taatttcttg taaacacgat agataatcat taaaacaatc agttactcct accttttcaa
      361 gacaacacat tcctgaaata agagtatctc taattataaa gaagttttct aatttagtta
      421 cctttttctg ttccaaaaga aagagcatta gcttttcgat ttgatttata aatttggcaa
      481 gatcttttct aatgagcatt tgataaaaaa acaaacgaat caacaacata tcatttgttg
      541 aataaacttc tttttctatg atctgagaca tatgttcctc aagtattaac tctgcatatt
      601 caggccttct agttctagaa gtatccaaac acgcctgcaa tacttcacat gctaattgtt
      661 cttctttagg aagattatca aagtatgttt tataaatctc ttccaaacac gcttccttct
      721 tatcgtactc ctcttcttta ccatagattg gttcacgtaa aatctggtat ttcaattcta
      781 aataagcagg aggaagagct gaaaaatccg gcataaggct ataaactgga actcctagcc
      841 ggcgagcaat atagtctaac ttgggcaaac tcggttgcga agctccactt tcaattcgcg
      901 acagttgacg aactgtcagt tcttgctcat ctccacaaaa atctggacga ctaattcctt
      961 tttctatccg aatctcttta attttttgac caattaacat cagacgcctc ctcttctacc
     1021 tgcttcgaaa aatactgtga aacttaaaaa attgtaacgg ctacatcttt cctactcata
     1081 agatttcata gcttcttcca cttctgccaa agtaacccca aacaactcta ggcgtttgag
     1141 gagttgcttg ccgttggaat agccgattcg gagagtctct ccgagatatt ctctacgctt
     1201 acggctgtct gcccctgcta gaaaaccaag gcgaatcaaa tcgctacgac taatgtcaaa
     1261 ctgactctca tgttcaaatt gttctgtcac ctgagctaga gccgttttca ggtcttcata
     1321 gctggcatgc tcaattccca gagaacgccc cttggtcttg gacttgggaa cagcttcatc
     1381 tcgcttgaga aaggcatgct gaactgttgg aatgaccgtc atgatcatgc gccgaatccg
     1441 ttccccatta aaatctgggt ctgtaaagac aatgactcca tgacgttggt gtagacgctg
     1501 aattcgctct atatcctgat cattgatggc agaacctcgt gtctcatagg tctccacatc
     1561 gaaataacgc ttgagattga ccgtatcata gcgaccttca accacgataa cttgagaaat
     1621 tttctctttc attacttgct gtccaatcca aaaattcgtt ctgcatttgc agtcgttgct
     1681 accgccagct cttctgtcgt cataccacgc aagtcagcga taaagtcgac cacatagcga
     1741 gtataggctg ttttattttc acgaccacgc ttgggtacag gtgctaagta aggcgcatct
     1801 gtttccacca acatcttgtc caaaggtaac tctttagctg cttcttggag gtcagttgcc
     1861 ttcttgaaag tcaccactcc tgagaaggaa atgatcatac caagatccac aaacttctct
     1921 gcccactcaa gcgtccctga aaatgaatgc atgataccac cacgaggacc aacgccctca
     1981 ctcttgataa tctcataggt atcttccagc gcatcacggg tatggacaac aaaaggcaaa
     2041 tccaagtcct tagatagctg aatctgacgg cgaaaaacct gctcctgcac ctctttgggc
     2101 gctgtcatcc aatggtagtc taagccaatt tcacctaaag ccacaacctt gggatgtttt
     2161 aacttatcca acaagtaagc ctcaacttcc tctgtataag taccagcttc tgtaggatgc
     2221 caaccaatag tcgcatagag ctgctcatac tcatctacca actccaaggc acgctcaatc
     2281 gtcggtttat caaaaccaac aatattcatc tgtatcacac ccatctcagc agccaaggca
     2341 atttcttctg cctcacgacc tgcaaattct tctacattca agtgtgtatg tgtatcaaaa
     2401 atcatctctt ctaacctcgt tttctatctt ctattatacc aaaaaagagg aggggcacct
     2461 aatttttcgg tttcccctcc tctcttcaat agagagctat tctgctatct tttctatccg
     2521 atattgccta tctcctattc cacagttaga gacagaagag attggctaca ttgatagaat
     2581 attcctattc agctaaagta tgtgattatc ttgggttctc acttttctca cccatcacta
     2641 ggattgatga cttctcaaaa tcccccttat aatctaactt taagtagaga atagaatttt
     2701 ctagctgttc tattttctac gtctagtctt tcttcctagc aaagcaaaac cacttgttgc
     2761 caccactaga ccaagactag caagtgctgc acttgatgct gaacctgtgt taggtaattg
     2821 tttgccagca ggtgactggt aggtaacatt ttgcattgtt cctgcagttg cttgcggtgc
     2881 acttgttgtc ttaggagcag cattaggagc cactagtctc ttaggaactg taaccatggc
     2941 aatggcttgc actccaatcg tttctgcttc cttcacatct tccatagttt tcgcattttc
     3001 aatctctttg agagctgctt gtttttctgc ttccactctt gctaaaagtt ctgctttttc
     3061 tttatcagaa agcggtgcgc ctttgatttc atcctgcttg ttcttggctg ctttttcaat
     3121 ggcattttta gctgattctt tttcagtagc taattgctta gaagcttgtg attgagctga
     3181 gttttgtgaa cctgtgtgag ctgatccacc cgcctggcta ccaccgcctt cattcgcatt
     3241 agcatctgag cttccagtat tgcctccctt atctccacct ggtgtagttt ctagcttata
     3301 ctctggtgta gcttcttgag ttacagattc acctttcgca gattgttgaa ctgtatcttt
     3361 tcctagaatc gtcactactg aaccatctgg gaatgtgatt gttgctgtac catctccacc
     3421 tacttcaact ttatagtaaa ataaaataat aacaggacaa atcgatcagg acagtcaaat
     3481 cgatttctaa caatgtttta gaaacagagg tgtactattc tagtttcaat ctactgtaac
     3541 atcgcttccg aatccatttg catctactga acttttttac aaaaataaaa atcagtagac
     3601 tcgagttcct actgattttt gatagaattc gtttggatta tgcagccaaa actttgcgtc
     3661 ctttacgacg acgagctgcc aatacgcgac gaccattttt agttgacata cggttacgga
     3721 atccgtgttt gcgcgcacga cgaagtttac ttggttgata agtacgtttc acgatgaata
     3781 cctcctcata gattttgtat tcgtttagcc ggctataaag tgatcagtta ctaaacatac
     3841 tttactattc tatctgattc tttcactttt gtcaatagct ctaggcaaat gtttccagct
     3901 ttttcgaatg caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga
     3961 tttgggctaa ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggctcctgcc
     4021 agttaaagcc tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa
     4081 actgctcatc atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc
     4141 ccgccttggg tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat
     4201 ttctttgctc gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgccccc
     4261 ccaaggaagt ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca
     4321 gcatattgag cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc
     4381 aacccacacg aaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga
     4441 agacatctgg tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt
     4501 catagatttc atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca
     4561 gcaagagttc cttaggataa agggctccag ttgggttatt tggattgata aggacgattg
     4621 ccttggtatt ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattcaacag
     4681 cttcatcaca gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata
     4741 gaggatagtc tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg
     4801 acatgacaat caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg
     4861 ggaatttctt cagctggcaa tactgcatga tggccttacg ggctgagaaa atccctttgg
     4921 agtcagaata gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg
     4981 gcgctgtaaa gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat
     5041 tcgctcgcat ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct
     5101 ctaacttact agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa
     5161 cattatacca ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat
     5221 agaagaaaaa ccttgctaaa ggcgaggtta agaacgtaga gatagcaggt attttgcaaa
     5281 gacacatcac ataccaaaag ataaacctag aaaaagcaag cctagaaagg agtcataaag
     5341 aagattaaac atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt
     5401 tacaactaga aataagatta gatggtagag tacatgaaag actaaaaatc caataaaact
     5461 tggataaaga agcggtacca gactctctaa ttgacagatc atgatcactt ctaccaagac
     5521 tgaaactagg ataattccat aataaaggaa atttgatttt tgaaaagagt ttttcatcat
     5581 tctcccttcc ttcacttctt tcctgctatc ctttcttt
//
LOCUS       DAVLSL010000119         5586 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLSL010000119 DAVLSL010000000
VERSION     DAVLSL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.121"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            57..245
                     /locus_tag="VXN30_001675"
     CDS             57..245
                     /locus_tag="VXN30_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1358270.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            307..1065
                     /locus_tag="VXN30_001676"
     CDS             307..1065
                     /locus_tag="VXN30_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1358271.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            1123..1977
                     /locus_tag="VXN30_001677"
     CDS             1123..1977
                     /locus_tag="VXN30_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1358272.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EHEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2089..2973
                     /locus_tag="VXN30_001678"
     CDS             2089..2973
                     /locus_tag="VXN30_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1358273.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3136..4875
                     /locus_tag="VXN30_001679"
     CDS             3136..4875
                     /locus_tag="VXN30_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV1358274.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLFDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIWKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
     gene            5150..>5586
                     /locus_tag="VXN30_001680"
     CDS             5150..>5586
                     /locus_tag="VXN30_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358275.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSENLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNT"
BASE COUNT         1874 a          834 c         1190 g         1688 t
ORIGIN      
        1 aaagccagtt gacacaagtg ttacacctgt gatagaatta acttgaggtg atgaaaatgg
       61 caaccgataa aaatagaata atgattagct tggatgataa aaatctagag aaacttgaaa
      121 atttagtaga agatgctaga gatagaaggg gaatgcgact aacaaagtcc caggttattg
      181 aattactcct aaataccgtt gattatttcg atgatattat gggggctatt tactcaaaaa
      241 aataagagca aaattgctat tattttttac aaattggtgt aacactagtg atataggagt
      301 gatgaaatgg ttgtttatat tagacaaagt aaattaccaa gtgaggtttc aattaataaa
      361 tacaatgctc aagtaggtgc ctacttacaa ggagaagaag ctgttttata tcaatctttt
      421 tctgaaataa aagagttaac aagtgaagat atagttgtag attatataat ggagactaga
      481 gcattactaa aaatgatggg cttaaacgtt ccggttcatg actatcctat tgagcttaaa
      541 gagttttatg gtcgaaagat ttatgctggt attttaggag agattgtgaa tatacctgat
      601 aattggggaa aatttattaa gcctaaagct ggttcaaaag tctttactgg aagagttgtt
      661 aatggaaccc atgatttaat aggtattggt ctacctttcg actatcctat atggattagt
      721 gaggttgtag aattcatagc tgaatggcgt tgttttgtgt tagatggtcg cgtattagat
      781 gttcgaccct atacaggtga ttatcatgca caatttgatg caagtgtaat tgatgaagcg
      841 atatcatgtt ggaaagatgc gccaatagct tatggactag atatcggtgt tactcgcgat
      901 ggcagaacac ttgttgttga agtaaatgat ggttatgcat tgggaaatta tggcctatct
      961 cctttaaaat caatcaattt tcatagagct aggtggaaag aaatggtaaa accctatttt
     1021 gaaaaaaatg aaatttttaa aatccaacag gatgttgttt tctaaaataa atatggtata
     1081 taaccaatat aaaaagcatc aaaatgaaca aggaggaatc tgatgcttgg agcaattatt
     1141 ggagatattg tcggttctgt ttacgaatgg aacaatatca aaacaaagga ctttccttta
     1201 tttcggaagg actgcttttt tacagatgac acggttatga cctgtgctgt tgcagaagca
     1261 attatgaatg gtggacaaaa agacgatttc attgacgcga tgaagaaata tggcagaatg
     1321 tatccgaatg ctgattacgg tgctcggttt aatgcatggc taaacagcga taaccgtgag
     1381 ccttataata gctttgggaa tggatcagct atgcgtattt ctccatgtgc ttggatcatg
     1441 gactgtggtt tttatgcgaa aactggtatg tggccatcat ctagaggact tacgagtctt
     1501 tctgcagagg taactcataa tcatccagaa ggtgtcaaag gcgctatggc tacagctgat
     1561 gctatctttc tatgtcgttt ttactttggt ggttattgta gagagcacga gcaatcaatt
     1621 aacgacaatc ctacagagtg taaaagacga attaaggatt atatagaaaa agaatacgat
     1681 tacaatctat ctcaaacttt agatgaaatc cgtcctaact atcgttttaa cgaaacatgt
     1741 caggaaactg tccctcaagc cattatcgcc tttcttgaaa gtagagactt cgaagatgcg
     1801 ataagaaatg ccatctcact tggtggcgac agtgatactc tcgcagcaat cacttgtagc
     1861 atagcagagg cagcttacgg tattcctgat tggatcaagg ataaggccta ttcttacttg
     1921 gatgaaccct taaaggatgt agttaggcga tgggaaaata gaataaaagc gtattaatct
     1981 ccagtacgtc agatttctct tttctgtatt ttcattgtat aataggatta catttacttg
     2041 catacgacga aatgtaaaca tttctgttta aaattaagga taaaatcaat gaaaaaagca
     2101 atggtaatta tcaaccctac ttctggtggc gagaaggctt tggattacaa agaaaagctg
     2161 gagaataaag caaaagaata ctttgaatat gttgaaacca aaattaccga aaaagcgctg
     2221 gatgcaacac attttgctga agaagcttct cgtgagcagt atgatgcagt ggttgtgttt
     2281 ggtggagatg gaactgtcaa tgaagtcatt tcaggtattg atgagagaga ctacattcct
     2341 aagttaggga ttatcccagg cggtacgggt aacctcatta caaaactttt ggaaatcaat
     2401 caagacatcg atggcgcaat tgacgaactg gattttgatt taaccaataa gattgatatc
     2461 ggtaaagcaa atgacaacta ttttggttat atctttagta tcggttctct gcctgaggcg
     2521 attcacaatg ttgaaataga ggacaaaaca aaattcggta ttttaaccta tgctgtaaat
     2581 accatgaagt ctgtcatgac agatcaggtc tttaacatta aggttgagac agaaaatgga
     2641 aattatgttg gtgaagctag ccatgttttg gtccttttga caaattactt cgctgataag
     2701 aaaatctttg aagaaaacaa ggacggctat gccaacattt tgattctgaa agatgcctct
     2761 atattctcca aattatccgt cattcctgat ttattaaaag gggatgttgt cgcaaatgat
     2821 aatatcgagt atatcaaagc gcgtaatatt aaaatctctt cagatagtga attggagtca
     2881 gatgttgacg gagataaatc agataaccta cctgtagaaa tcaaagtcct agctcagcga
     2941 gtagaagtat tttcaaaacc gaaagaggat tagtatatag agaaagcctt ttttaaggct
     3001 ttttgtatac tttaaaagat agttccttta acaacggaca ttccttgcaa atagtttaca
     3061 aaaatagtat actggattta ttgagtttga aaacgtttgc gtaaaatttg aatgaatact
     3121 ttaggagaca aattgatgga attgagtgct atttaccata ggcctgagtc ggagtatgcc
     3181 tatctttata aggataagaa actccatatt cgaattcgaa ctaagaaagg ggacattgaa
     3241 agcatcaact tgcactatgg ggaccctttt atctttatgg aggagtttta tcaggataca
     3301 aaagaaatgg tcaagataac ttctggtacc ttatttgacc attggcaggt tgaagtgtca
     3361 gttgactttg cacgtatcca gtatctcttt gagctcagag atacagaagg tcaaaatatt
     3421 ttgtatggcg ataaagggtg tgtggaaaat tctctagaaa atcttcatgc aattgggaat
     3481 ggatttaagt tgccttatct tcatgagatt gatgcctgca aggttcctga ctgggtttca
     3541 aatacggtat ggtatcagat atttcctgaa agatttgcca atggcaatgc tctattaaac
     3601 ccagaaggga ctttagactg ggattcatct gtcacaccta agagcgatga tttctttggt
     3661 ggtgatttac aggggattat tgatcatatg gattacttgc aagacttggg tattactgga
     3721 ctatatcttt gtcccatctt tgaatctaca agcaatcaca agtacaatac gacagattac
     3781 tttgaaattg accgtcattt tggagacaag gagacctttc gggaactggt ggatcaagcg
     3841 catcatcgtg gcatgaaagt catgctggat gcggtattta atcatattgg ttcgcaatct
     3901 cttcaatgga aaaatgtcgt caaaaatggt gaacagtctg cttataagga ttggttccat
     3961 attcaacaat tcccagtgac aactgaaaag ctagttaata agagagactt accctatcat
     4021 gtttttggtt tcgaggacta tatgcctaag ctaaatacag ccaatccaga ggtcaagaat
     4081 tatcttttaa aggttgcgac ttattggatt gaagagttta atatcgatgc ttggcgtttg
     4141 gatgtggcta atgagattga ccatcagttc tggaaggatt ttcgtaaggc agttttagct
     4201 aaaaatcctg atctttatat cctaggagaa gtctggcata catctcagcc ttggctaaat
     4261 ggagatgagt tccatgccgt catgaattat cctttatttg atagtatcaa ggactatttc
     4321 ttacgaggaa ttaagaagac agaccagttc atcgatgaaa tcaatggaga gtctatgtat
     4381 tacaagcagc agatttcaga ggtcatgttt aatctcttgg attcacatga tacagagcga
     4441 atcctgtgga cggccaatga agatgttcaa ctggttaaat cagccttagc ctttctcttt
     4501 ttacaaaaag gaacaccgtg catttattac ggaaccgagc tagccttgac tggaggacca
     4561 gatccagatt gtcgtcgttg tatgccttgg gaacgtgtat caagtgacaa tgatatgctg
     4621 aactttatga agaggctgat taagatttgg aaatacgcgt cagtaatcat ttcgcatggc
     4681 aagtatagcc ttcaagaaat caaatctgat ctagtagctc tggaatggaa atacgaagga
     4741 cggatcctca aagcaatatt caaccaatca acagaagatt atcttttaga gaaagaagca
     4801 gtagcactag caagcaattg ccaagaattg gagaatcagc ttgtcatctc tccagatgga
     4861 tttgtgattt tctaaaaact agttgatgaa gattatggta catttcatat cttatatagt
     4921 ataataaggc tagttactaa acttgtaaag gagaacttaa atgaattgta gaggacatga
     4981 aacaagacaa agaattgtta gagattttga agtttagcct aaagcacata ttaagctgtt
     5041 agcaaatcaa caaaaacata gtgatgcagg agcaactatt gaagatgaat attatgtatt
     5101 tatcgctgag agtaaaattg atggcaagaa ggaagttatt cagtgttgca tgggtgcggc
     5161 aagggatttt ttagaactaa ttaatcacaa agggctacct ctttttaatc cgcttgtagg
     5221 tgattctcat gtaaataata gacaagaata tgacaataca gggagtgaaa atttacaacc
     5281 tgaaaagtgg aatgaaactg caaagcagct ttataatgct ataatgtggt tgattatttt
     5341 atggaatgct aagccggata cacctttatt taattttaaa gacgaagtaa ttaagtataa
     5401 aacatatgag ccttttgaaa gcagtataaa aagagtaaat actactataa agaatggtag
     5461 taaagggaaa actctgactg agatgattaa tggctacaga gcggataacg atattagaga
     5521 tgaaatttgt aactttaata ttctgaaaaa taaaattcgt gatatgaaag accaacaagg
     5581 aaatac
//
LOCUS       DAVLSL010000120         5510 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLSL010000120 DAVLSL010000000
VERSION     DAVLSL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.122"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(3..935)
                     /locus_tag="VXN30_001681"
     CDS             complement(3..935)
                     /locus_tag="VXN30_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1358276.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(932..1789)
                     /locus_tag="VXN30_001682"
     CDS             complement(932..1789)
                     /locus_tag="VXN30_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1358277.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSHNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(1793..3139)
                     /locus_tag="VXN30_001683"
     CDS             complement(1793..3139)
                     /locus_tag="VXN30_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1358278.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(3152..3436)
                     /locus_tag="VXN30_001684"
     CDS             complement(3152..3436)
                     /locus_tag="VXN30_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1358279.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(3440..5470)
                     /locus_tag="VXN30_001685"
     CDS             complement(3440..5470)
                     /locus_tag="VXN30_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1358280.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         1848 a         1167 c          748 g         1747 t
ORIGIN      
        1 atctatcttg cattataaat ttctagaacc ttctctttta tattcgattc actcaaacca
       61 tactcattaa gaagataatc cattttccct acttgaccaa atctttcttg aacacccatc
      121 cgatgaattt ttgttattcc atcatcagag aataattcac ataaagcact gccaattcca
      181 cctatctgat tgtggttttc tacagtaaat atagtttttc cacttaacat tgtttttatc
      241 tgttctggta tcggtttgat tctaaataaa tctatcacac ctactgaata acctaattta
      301 gacagttcat ctgcaactcg aatacttgga gcaaccatta tgccagaagc aacgattaca
      361 agatcttcac catgtcttaa ctcaatgtag cctttagaaa aatcttctcc accttgatat
      421 acagcctctg gagcttttct aattgttcga atatatttta gtccttttaa gtctaatgtc
      481 tgattcaata tttcacgaaa ttggatatca tcagttgctt cgaaaatgat tgatttagga
      541 attaaacgta acaagccaat ttcttcaaat ggcatatgtg tcccaccatt catttctgca
      601 gttactcctg catctgatcc aatcacagtg gcatccaatt gtgcgtatcc aagagaaata
      661 aataattgat caaataccct tcgtgaagca aaaggaccaa atgtatgaag ataaggtcta
      721 aacccctgaa tagacaagcc tgctgcaagg ccgaccattt ctgcttccat aatcccaaca
      781 ttcacataac ggtctccaaa gtccttttca agattattag tagccatcga acttgacaaa
      841 tcggcttcta agactactat atcagaatca ctttgattag cctctaaaag gaagtctcta
      901 tatacatgcc gtaattcttt cgtacttctc atcattctgt ttcctccaat tcctgactta
      961 atctttctac aactgaagtt aacatttgtc tctcctctac agtagggcga agatgatggt
     1021 tggatttcat ttcttccagc tcttgaaccc cttgaccttt aatagtatct aatacaatgc
     1081 acttaggtga tgaattatgt gactgtttta attggacaat cccttcataa atttctctaa
     1141 tatctgaacc cttgacccta atggattcaa atccaaatgc tgaaaatttt tctacgaaat
     1201 cacctggatt acaaatatcc tttgtaaaac catctaattg ttttttgtta tcatcaacaa
     1261 atacaattaa gttggataac tgttgatgag aagcaaactg tatagcctcc caacattgtc
     1321 cctcatttaa ctcaccatct ccaacaatag cataagtata aaagggactc tttcttattc
     1381 tctgaccata tgcaagtcca gttgcaacac taattccttg tcctaaagag cccgttgtca
     1441 tatctatgcc tggcgttaga tttctatcag gatgagacgg taatttggtt ccatttgtat
     1501 ttaaagaata taagaattct ttgtcaaaga aaccattcaa atagagtgta ctgtatagag
     1561 ctggtcctcc atgacctttt gataatatga aataatctct atctcgtgct gcaaatattt
     1621 ctggagtcat tggcattatt tcaccataaa gcactgctaa aacttctacg atagacagac
     1681 ttcctccgta atgtccgaat ccaagatgat tcaatgttct aagagtattt aatcggatgt
     1741 tagtcgcaaa ttttcttaac ccatcttctc tatttttact taaaatcatc ccttattcct
     1801 cctttgcaga tggcttttta ataaaggata ctccaaacat aactgctaaa ataagaacaa
     1861 gaccaatcac aatgcctgct tgtgagccaa attgatttaa cattcctaaa ataattcctg
     1921 atagaccaaa atctgcatct gagaaagttg atccttggaa accgagtcct cccaaaactg
     1981 gcattaaaaa gactggaaga aaactgatta aaataccttg taaaaatgct ccaatagtgg
     2041 ctccacgaac accaccagat gcattcccaa tgacacctgc agtcgctcca cagaagaaat
     2101 gaggcacaac acctggtaag ataacaaccg ttcctgaagc aatcataatt gccatactta
     2161 ctaaaccacc aataaaacta gagataaatc caattagaac tgcattgggt gcataagtat
     2221 aaacaatcgg acaatccaaa gcaggttttg aattaggtac aagacgctct gaaatacctt
     2281 taaaggctgg aacaatttcg cccaaaataa ggcgaacacc tgctaaaata acaaataccc
     2341 ctgctgcaaa ttgacctgct aattgtaaag cataaactag accacttgta ccactactga
     2401 tttctttttc tatatattct gaccctgcaa agatagctac aataatgtaa ataactgcca
     2461 tggataaagt aatactaaca gtactatcac gtaaaaaagc taaactcttt ggaaatttaa
     2521 tgtcctctgt tgattttgat ttgtcaccga taaggctacc agtaaaacca ctcaaccaat
     2581 atcccaaaga actgaaatga cctaaagcca ccttgtcatt tccagttaat tgaaccatat
     2641 atttttgaac aaatgctggg gaaatactca taataatacc gagtgctaat cctcctagta
     2701 agataagagg caagctagta aagccagcaa ctgataaaat gaccgcaatc atacacgcca
     2761 tatatagagt gtggtgccct gttaaaaaaa tatatttaaa tcgagtaaaa cgagcgatta
     2821 agatattgaa caccatgcct gcaaacataa tcagtgcagt agctgagcca tatgttgtta
     2881 aagctacagc tacaattgct tcattattcg gcacaacgcc agataaatga aaagcatgct
     2941 caaacatggt accaaatgga tttaaagaat tttgtacaat tcctgcacca ccagatacaa
     3001 ctaagaaacc aacaaaggtc ttaattccac ctttaataat atcaggtaat ttcttcttct
     3061 gaagaactaa tcctaaaatt gcaattaaag ctactaaaat agctggtgta ctaacaatat
     3121 ccaatatgaa cttcatcatg acgctagcct cctatataag tcctttttct tcacaaagtt
     3181 tagtaattaa ttctcgtagt tcatccatat caataatact atttaagata cgaacatctc
     3241 caagatgact agctgaatca gctagatcac gaccaacaat ccaaatatca gctgcatttg
     3301 gatctgctcc acctaaatca taatgttcaa cttctacatc cgaaacattc aaatcactca
     3361 atacagattc aatattcatc tgtaccataa aacttgaacc taatcctgaa ccacaagctg
     3421 taccaatttt taacattatc taatcctcct gtttaattat cattttaatg tcatcatagt
     3481 tttttgatga aattaaagtt tgaacatgat ttttatctct taaaattgtt gttaaatgtg
     3541 acaaagcctt taaatgactc tcattatcaa tggctgcaat acaaatcaac aatcttacct
     3601 cttgttctgg attatccaat aaataaatcg gttcttccaa aactaacatt gacattccta
     3661 tttcattcac accttcatct ggccgagcgt gaggaattgc tactcccttc cctaaattaa
     3721 taaaaggccc aaactcttct actttttgaa tcattgcctc agggtagttc tcagttatct
     3781 tatcttgatc caaaagcggt ttagctgcta aacgaatcgc ctccttccat cctaattttt
     3841 gcgaactaac ctgataggtt tctttggtaa tgagttgttc tagcactggt acaatttcct
     3901 ttctatcatt tttttggtaa agataattct ttaacgccaa tcttaattcc aattcttgtg
     3961 taataattcc atatcttttg acaatattca ggatttgttc aatctcaaaa tccccatact
     4021 ctaaattcgg aaaatctttt aacactagtt ctactagttg tattgcttgc tcttcagtca
     4081 tcataaccga aactagataa tttggctttt ctgtctccac ctttatggta gaaaaaacca
     4141 tatcatagtc actactagct ttcacctgta aatcatcaat ctttgaggtt cctataaact
     4201 caatttgagg aaataatgct aatagattct cttttaacat caatgaagaa ctaacaccat
     4261 taggacaaat gattgctgct ttatagcatt tttgaggcaa agtatctgct ttctttaaat
     4321 aacctccgaa atggataaca aaatatgctg tttcactatc aggtatggga ttgtcaatag
     4381 cgtccatcaa gggcatcaaa gaatctttga ctaattcaaa taaatcagga taatgttctt
     4441 taacatgcaa tacatattca tttgaactag gtaggccgaa ctttaatcta tagtaagccg
     4501 gtataaggtg gcggcgaaga ttttctctca atccttccct ttgtttaaaa tgtaacaaag
     4561 aaatatcttc cattctactt ataatagcct ctgttaattg attaaagtaa accggagcaa
     4621 catctacttc accttcaaag caacttgata ataaaacggt gatatagcga taatcatcct
     4681 cagaaaacac cgtatctata attcccaaat caaccactgt atccaataaa atagtggtta
     4741 tatcttgaat aacaggagat actaatgtct ctgaaagaca tactctttca acatcccttt
     4801 gatacctaca cagaatgaat actaaaccga aaaggcaaac ttttaattga ttaacaatag
     4861 gtactagctg tagcttctca taataatctt taactacctg atttatcaaa tcataagtta
     4921 atgaataccc ccaactggat aaaacataat ccaaacccca aatccctatg gaggattcca
     4981 gcaactcact aaccatttga aaagctaagc ggtgcttatt ccactctgaa ccgtgtaaag
     5041 tataaccttt tgctctactg taccctagct ccaaatcatt atctaacata atctttctta
     5101 atgattgaat atcagataag gttgtattct tacttacttt caaaaagtct tggtaatgat
     5161 tattcgatat aaaatctaat cggcaaaaag tgtaaagata gattaaagct aagcgagtcg
     5221 actttggtaa aaccaattca tccgacttaa taatatctgt caaagactgc ttcgtacgat
     5281 ttgataaact atagcgacct tgctttttat ccagcactat ccctttatta gctaaataag
     5341 gcactaaata atctattcct tctttgactt cctttatagg taagctcacc ttaacagata
     5401 actcatataa tgatagctca caatgatcca tcaaagtcat caaaataact agtgctctat
     5461 aatcaaacat ttctcttcct ttctaactac aattatagtg taaaagttat
//
LOCUS       DAVLSL010000121         5487 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLSL010000121 DAVLSL010000000
VERSION     DAVLSL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.123"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(133..1101)
                     /locus_tag="VXN30_001686"
     CDS             complement(133..1101)
                     /locus_tag="VXN30_001686"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1358281.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1246..2029)
                     /locus_tag="VXN30_001687"
                     /pseudo
     CDS             complement(1246..2029)
                     /locus_tag="VXN30_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(2054..2551)
                     /locus_tag="VXN30_001688"
     CDS             complement(2054..2551)
                     /locus_tag="VXN30_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1358282.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2624..3985)
                     /gene="radA"
                     /locus_tag="VXN30_001689"
     CDS             complement(2624..3985)
                     /gene="radA"
                     /locus_tag="VXN30_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1358283.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(3999..4514)
                     /locus_tag="VXN30_001690"
     CDS             complement(3999..4514)
                     /locus_tag="VXN30_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1358284.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4516..4959)
                     /locus_tag="VXN30_001691"
     CDS             complement(4516..4959)
                     /locus_tag="VXN30_001691"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1358285.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            5264..5413
                     /locus_tag="VXN30_001692"
     CDS             5264..5413
                     /locus_tag="VXN30_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358286.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
BASE COUNT         1714 a         1323 c          969 g         1481 t
ORIGIN      
        1 atgacgtaca gtcatatttc ttctcttttt attatatcac agttttaaat ctagctttac
       61 tagattcacc gctactatct atttattcgg aaaaaagacg aaaaaacctg agaatcatct
      121 caggcttggt cattaaattt ttttctcaat atcgaaaagt ggagaaagtg gtcgtttttc
      181 atgaatacgt acgatagcat cccctaggag atgagcgatt gaaatctgct caatcttatc
      241 aatcaaacgc tcttctggaa gatagatggt atccaaaaca accaatttct taatagctga
      301 tttttggata ttgtccgtag caggaccaga aagaactggg tgcgtacagc ttgcatagac
      361 tgcaacagca ccagcttccg caagagcatc tgccgcatga caaatcgttc cagcggtatc
      421 aatcatatca tcaatcaaga tacaagtctt gccttcaacc ttaccgatga tgttcataac
      481 ttcactagta ttcatcttat caacgctacg acgtttatca ataatagcga tagatgtttt
      541 caaaaattct gccaacttac gagcacgagt cacccctcca tggtccgggc tgacaaccac
      601 atagtcagaa ccaaccatac cacgccgctc aaaataatct gcaattagag gagcacccat
      661 caaatgatcc acaggaatat caaagaatcc ttgaatttgc gcagcatgca agtcgatggt
      721 caataaacga tccactccag ctacttcaag catatttgcg acaagttttg aagtgattgg
      781 ctcacgcgct ctcgcctttc tatcctgacg tgcataccca tagtaaggca tgacaacatt
      841 gacagactct gcactcgcac gcttcaaagc atctaccata atcaaaattt caagcagatt
      901 gtcatttaca ggcgaactag ttgattgtaa gataaagacg tgtttcccac ggattgattc
      961 ttcaatgttg acctgaatct ctccatctga aaattggcga acacttgatt tccccaactc
     1021 tatcccaatc tcctgcgcca cacgttctgc caattcttta ttagaagaaa gggcaaacag
     1081 ctttaaatca gaaaaagaca tgatttcctc cggtatatat gtatcgcttg tgcttttcac
     1141 aagattttcc atctaccatt gtagcgcttt ttgcactatt tttcaatcaa aaataaaaga
     1201 agggcaccat atttgtaccc ttgcatcatt cttttgaaaa atattctagg tcatcaactc
     1261 attgtgtttc tcaacaaagc aataagcatg ataataacca tagagagcaa tagccgtaac
     1321 cactggaatc gctaaaggca actctgtttc caactccaca aaaggagagt taaacaataa
     1381 gaccctgttt cttgccaacc ttctgtcctt tataggctct gtaaagcaag taaacaccta
     1441 ctacagctag acctgaaaaa gtccagtgag aggcaattcc tgagatgata cgctctaaaa
     1501 ttcgcgaaat agtaaagtca aagccctctg gcaaatccgt acgaatataa ccaatatcct
     1561 caatcatttg gaatcccaaa ccggaggcaa ttccaagtaa aaacaaaaat tttaattttc
     1621 gcacaggaat caaagccaaa acaaaaacaa gtggcaataa tttcaagggt tcttctacca
     1681 aaggagccac aatagcactt tcaaaggcat ttaaaaatgg actatctggg aaaagaaccc
     1741 ccagtaaatc atgtatataa gtattagcaa aactagacaa ccagcctgaa aggaacatcc
     1801 ctcccaataa agacagaatc aaaaccttct ttggcaattc ccattttttc ccaatacgga
     1861 agagaaaata aagagccgga atcatgtaaa agagagctag aaagatagaa actcccatta
     1921 gtccatattc cgcacctgac ctcgaaccgt ccgtatagta gatggtttca tactgtaaac
     1981 caatacatag caataaaata aaaataaata aaatactgct tttcttcata cactttcttt
     2041 ctaaatgaag tatttataat tctacgactg tcatacttcc tgtatcaaca ttgtaaatag
     2101 caccagagat aatgacatcg tctggtatta ggggagactc gataagcagt tgcatatcct
     2161 cgcgtacact ctcttctata tcttggaagg gcaagaagtc ctggtctgac acatctacac
     2221 ctaattcctc ttttaaatac tcctgaaaag gttcattttc aaaggtctga gcaccacagt
     2281 ctgtatggtg caataccaca atctctcttg tccccatttg ttgctgggaa ataactagcg
     2341 aacgaatcat gtcttcagtc actcgaccac ctgcattccg caagatatga gcatccccaa
     2401 gtgccaagcc cagagcttgc gcaacgtgca aacgtgagtc catacaggtc acaatggcta
     2461 ctctggtttt aggtttaagt ggcagattta actgcccatg tagggcaaca taagcctgat
     2521 tggcttgcat aaactgttca aaatacgaca cgattccctc cttgaaaatt tgatagtcaa
     2581 atatttctcc tatcttatca tttttaagag aatttgtcac ggattatgca aagacctttt
     2641 tcaagacttc ctgaatcgtt gtcacgccaa tgacctgaat ttccttaggc agagtgattc
     2701 ctgtcaagga attctgaggt acataaatct tagtaaagcc cagtttagca gcttcgttga
     2761 tgcgttgctc aatacgattc acgcgccgaa tctctcctgt caagcccagt tctccgacaa
     2821 aacattcctg aggattagtt ggcttgtctt tgtagctcga agcaatagca actgcaacag
     2881 ccaagtcaat ggcaggttca tccaatttaa caccaccagc agatttgaga taggcatcct
     2941 gattttgcaa gagaagccct gcccgttttt ccaaaacagc cataatcaag ctagcacggt
     3001 taaaatcaag tcctgtcgta gtacgcttgg catttccaaa catggtcggt gttaccaaag
     3061 cctgaacctc cgccaaaatc ggacgcgtcc cttccatggt tacaacgatg gaggaaccag
     3121 tcgccccatc caaacgctct tctaggaaaa cttgactcgg attgagtacc tcaaccaagc
     3181 cgcccgactg catctcaaaa atcccaatct cattagtgga accaaaacga tttttgaccg
     3241 ctctcaaaat acgaaaggtg tggtgacgct ccccttcaaa gtaaagcacc gtatccacca
     3301 tatgctccaa catacgaggc ccagccaagg ttccttcttt ggtcacatga cctacgataa
     3361 agatggcaat gttattggtc ttggccaact gcatgagttc agcggtcact tcacgtacct
     3421 gagaaacaga cccctgcacc cctgaaatct caggagacat gatggtctgg atggaatcaa
     3481 taatgagaaa gtctggctgg atacgctcca cttctgcacg aacactctgc atattggtct
     3541 ctgcatagag ataaaactca ctatcaatat cacctaagcg ctctgcacgt agtttaatct
     3601 gctgggcaga ctcctcccca ctgacataga gaactgtccc cacttgggac aactgggttg
     3661 agacttgtag gagaagagtt gatttcccaa tcccaggatc cccaccgatg aggacgagac
     3721 ttcctggtac cactccgcct ccaagcacac ggttgaattc ctccatctcc gtcttggttc
     3781 gattgacatt gatggaagtc acctcagcta gtttcatggg cttggttttc tcacctgtca
     3841 aggacacacg cgcattcttg acctcggcaa cctcaacctc ttccacaaaa gaagaccaag
     3901 acccacagtt ggggcaacgt cccagatatt taggggaatt atacccacaa ttttgacata
     3961 caaatgtcgc ttttttcttt gcgatgacaa acctctttct atatctctaa ctcacactca
     4021 atcacttggc aaaaatcaat cttctcattt ggcacaaact ggcgcatgag cattcgatga
     4081 gcaacaacta ccacagtctg atgttctcga tacttagaca tacattctag aaaccgagac
     4141 ttcatttccg tagctgtctc atattgaata ggactattag gaagcaactc ccccttgttt
     4201 tctaaaaaca gtcttctagc tgtttcaaag ttttctattc ctgttttata gacctgccat
     4261 tcatgtaata aaggctctac tcttaaagga agacccgtag cacagaccac atacgaagcc
     4321 gtttctaaag ctcttgtgac tgcagaagat acgattattt cagctgacga gagtaaagga
     4381 tttttgctca atttctggac ttgctgccgt cccatctcag acaagggtgc caaatctatc
     4441 ccaaatccta tataagaacg ctcctctaac tcacggtaat ctggctcccc atgacgtaca
     4501 aagataatct tcattctagt gccctgtcga tccaaatcca ccagttcgaa cgccatcagc
     4561 tgcatctcca tctgcaatta agaaagtagc aaaaacagcc tggacaatac gttccccaac
     4621 ttcaagaaca acctcttgat ctgtgatatt cttcatctgc gcaaaaatat gcccttcatt
     4681 tccaggattt ccataataat ccccatcaat gaccccaact gagttaatta aaaccaagcc
     4741 cttcttacga ggatttgaag aacgatcata gaggtagaga acctcagtcg gctgcatata
     4801 agccttaacc cctgtcggaa ccaagacaat ctctcctggc gcaacaactg tacgcacagc
     4861 aacctttaag tcgtaaccag ccgcatgcgc tgtctcacgc ttgggcaata aattttcatc
     4921 tgtaaaactc gaaaccaatt caaaaccacg aattttcata attttctctt ttctattatc
     4981 atttattcta gattattcta tcttatttat tcggaaaaag cacgaaaaaa agagcacaca
     5041 acaattatag gcgatacgat aatttacact gtttcacaat acgttgaaat ttagagcttt
     5101 aaagcgaggg caaagttgat tttttacatc gttttacaga ggtttacgac acttttgccc
     5161 cttttttgcc cctttgtttt tcaaaaaaac ttcatcaaat cgcttgactt tcacggttta
     5221 ccgtgatata atataatcaa gataaggaaa ggaggtgagg aagttgaaca aagaagattg
     5281 gcttaggtta ctggaaaagg cgatagataa tatccctgaa acggtaactg ctatagcaag
     5341 tctggtgact gcaataacgg tcgcaaggca aaacaaaaag cgtaaaccga aatcccgtca
     5401 aagaaaaagg tgaacgctaa gaggttgggg cgaaagcccc tcacacctct attttatcaa
     5461 atgaaaagag gaaaagcaat ggttagt
//
LOCUS       DAVLSL010000122         5436 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLSL010000122 DAVLSL010000000
VERSION     DAVLSL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.124"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            45..1040
                     /locus_tag="VXN30_001693"
                     /pseudo
     CDS             45..1040
                     /locus_tag="VXN30_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            1066..1710
                     /locus_tag="VXN30_001694"
     CDS             1066..1710
                     /locus_tag="VXN30_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1358287.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFTAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1752..3740)
                     /locus_tag="VXN30_001695"
     CDS             complement(1752..3740)
                     /locus_tag="VXN30_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358288.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFTKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3742..4500)
                     /locus_tag="VXN30_001696"
     CDS             complement(3742..4500)
                     /locus_tag="VXN30_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358289.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGIFYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4928..5119
                     /locus_tag="VXN30_001697"
     CDS             4928..5119
                     /locus_tag="VXN30_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358290.1"
                     /translation="MKHKEHILIGFLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1705 a         1079 c         1064 g         1588 t
ORIGIN      
        1 tgttatactt gttgttcaac cacaaacaca agaaaggcac agagatgcaa gacaattata
       61 ctacaaaagc caaacatttg acaatcgata gccgtcgctt aatcgaaaga tggaaaaaag
      121 aaggaaaatc aaatagagaa attgcctctc tacttggaaa agctcctcaa actatccaca
      181 ctgaaatcaa gcgtaggaca gtccgacaat gtcttggaaa agggcgcttc aaagaggttt
      241 attctgccga ctatgctcaa cagtcttatg aaaataatcg caagcactcg gtcaagagat
      301 caagcgtgac caaggaacta aaggaaaaga ttctccacta tcataaccaa aaattttcgc
      361 ctgaaatgat ggttatggct aaaggggtta acgtgggaat ttcaaccatt tactattgga
      421 ttcatcatgg aaaattgggg ttaagcaagc aggatctgct ttaccctaga aaaggaaaag
      481 cgcttaagaa acaggctagc accaacttta aacctgctgg tcaatccatc gaacagcggc
      541 ctgaagctat caatcttcgc ttggagaatg ggcattatga gattgatacg gttctactta
      601 cgagatcgaa aaactactgc ttgcttgtct tgacggatcg aaagagtaga catcagatca
      661 tccgattgat tccaaataaa agtgctgagg tggtcaatca ggctctaaaa ctcatcttaa
      721 aacaacacaa gattctttcc atcacggcag ataatggaac ggaattcaat cgcttgtttg
      781 atatattttc tgaggagcac atctattatg cgcaccccta tgcctcttgg gaaaggggaa
      841 ctaatgagaa tcacaacagg ctcattcgta gatagttacc taagggaacc aagaaaatga
      901 ctcccaaaga agtcgcattc atcgaaaagt ggattaacaa ctatcctaaa aaatgcttgg
      961 actacaagtc acccagagaa gacttctgga tggctaactt gaacttgaaa tttagcgtca
     1021 gaaacaaatc acgaaattga ttcaaatttt gcaggtcgtt taaatatcct gcgtgcgggt
     1081 gttcttgatg ctaacgatgg aattatttcc attgctggtg tggttatcgg agttgccagt
     1141 gccacgacca atatctggat tatcttttta tcaggtttta cggctatctt agctggtgcc
     1201 ttttcaatgg ctggtggaga atatgtatcc gtttcaactc caaaagatac cgaggaagct
     1261 gccgtttcgc gagaaaaact cttgctagac caagataggg aactagccaa aaaatccctc
     1321 tatgctgctt atatccaaaa tggagaattc aaaacttctg cccaactctt gaccaataag
     1381 atctttctta aaaatccact caaggctctg gtagaggaaa aatatgggat tgagtatgaa
     1441 gaatttacca atccttggca cgctgccatt tctagcttcg ttgccttttt ccttagaagt
     1501 ttgcctccaa tgctgtcagt gaccattttt ccaagtgatt accgcatccc tgctaccgtc
     1561 cttattgtcg gtgtggccct tcttctcact ggttacacaa gtgctagact tggaaaagcc
     1621 ccaaccaaaa cagctatgat tcggaacctt gctattggtc tcttgaccat gggagttacc
     1681 ttcctgctcg aacaactttt cagcatttag aatacaagaa atacctcgat tttgaagtcg
     1741 aggtatcttt tttacatttg cacaatcttg cgataacttc ttgaagtaat catgaaaatc
     1801 agcacatagg cgatgaggaa gatagcgcag atagacaagg tcacaatcaa catcatagtc
     1861 gtatccagta caccaatcac ttttaaaatc aggctaagca tatggtaggc aaaggcgaga
     1921 tgtatgaagg caaagagcaa aggaaggaag aaaacagtta aaacctgttt gttgatggtt
     1981 tgcttgattt gcttttggtc caaaccgact ttctgcaaga taataaagcg ttcacggtct
     2041 tcgtagcctt cagaaatttg tttgtagtag atgaccagaa cagttccgac cataaagata
     2101 atggatagga aaataccgat aaagaagaca ccaccaaaga gggcactcat ctgagaacta
     2161 gcatctgcta gattgctacc ataaacatag ctaccttctg tgtctaattg agcattaaat
     2221 tgattgaggt agttttcata ctcctcagcg accttgagtt gttcttcttc gctggcatct
     2281 acattcatac caccgtaaaa ctgattatag atagccgaat ctgggaattg atccaaaaag
     2341 gcttgtaaat caggaacaac aaggtaattg taatcagtag tcaagatatt aaacttattt
     2401 ggaacatggt tgacaataaa atctttagta aattcttctt tgacagaaaa ttgatgatca
     2461 tttagagtta gagctttctg tcctttcagt ccgtcatttt tggcaaagag accgacctca
     2521 tttcctgata gagacagttt ttgaccagtc atattttcat aatctttttg gtcaaatacc
     2581 atgaaaactg tttttggttg gacacggttt tgtccttttt caaaaatagt taacttggtt
     2641 ccttcttgat tcgcaacagc aaagtaagtg taacgaagca cttctttctc tttaatctta
     2701 taacctttgt cacttgcaaa ctggctcaag agtttgtcca aatcttcttt ttcaacattt
     2761 tgccctgaaa ccccaaaatc atgaggattt agaacttttt taaagctttc tgaggaattg
     2821 aaaatgcttg tcgctgctga catggttacc aaaaccattg ttgacaagat agcgatggtg
     2881 gctagtccaa ctgcattttt cttcatacgg aaaatcaagt tagaaacaga tatgagatta
     2941 tttggttggt aatagtattt cttatttttc tttaagattt ggaggaaaac ggtaatccct
     3001 gcattaaaca agagataagt cccaaagata accagtaaaa cagctatgaa gaaggttgtt
     3061 aaggctgtaa gaggatcttt taccgtaagg gcaagataat agccaattcc taaacttatg
     3121 gaaccaagaa tggtttggag aggaaggaag cgaccttttt tctctccact agctttctca
     3181 cgagagagct ggagggcatt catacgggcg attcgaaggg cattcaggaa catgaggcct
     3241 aggaaaatca aaccgaagac aacaagcact gtaatgacaa ctttcgtctg gaaggtagca
     3301 accagctcaa ccttcaattt cattagtttg agcaggaaag cgaaaattaa cttgtcaaac
     3361 aaggctccaa taccgatacc cgctccaaca gttagaatcc caaataccac taactcctta
     3421 aaggtcatac tgataagatg acgcttctcc aagcccaaca tgccataaat tcctagttcc
     3481 ttggaacggt tcttcatgac aaaactattg gcatagagaa cgataatggc tgacgcaagg
     3541 gtgacgacaa acataccaaa tccaagtgta gcctgaatgg ttgttcctcc acggatttcc
     3601 gcaatcttag gattgaaggt tagagagtaa aagagatagg tgacagtgac tgccaagaga
     3661 acagccagcg caaaaggata gtagagtttg cggtttttaa tcaagttcga taccgctaac
     3721 ttattggtta atcgaaacat actaattcac ctcgcttgcc atgacagtca aggtatcaga
     3781 gatttcttgg aacatctgac gttctgtctt ctctccacgg tagatttgat tgtaaaaaat
     3841 gccgtctttg ataaagagta cacgtttagc tctgctagct gctgctgttg agtgggttac
     3901 catgagaatg gtttggccgc gctcattgat ttcatcaaag acatcaagta aggctgcaga
     3961 tgacttggag tcaagagctc ctgtcggttc gtccgcaagg agaatttcag gttcagtgat
     4021 gatggcgcgt gctactgcta cacgctgttt ctggccacca gaaatctcgt aagggtactt
     4081 ctcttgtaat tggtttattc ccagattctc agctgtcacc accaatttct tcatcatctc
     4141 tgtaataggt cttcttgaca agacaagcgg aagcaagata ttgtccttaa cagacagagt
     4201 atctagcaag ttaaagtctt ggaagacaaa tcctagcttt tcacgccgga aactagaagc
     4261 ctgtgaattt ttaatagttg ctgtatcagt tccgtttagg taaacctgcc cacgacttgg
     4321 tttatccagc atagctagga tattgagcag agtggattta ccagaaccag actcacccat
     4381 gatagcaacg tagtcaccct tttctacggt gaagtgaata tccttgaggg cctctacttg
     4441 gttgccctga aaacgagttt tataaatttt ttgaacgtgt tttacatcta aaagtgtcat
     4501 gagaatctcc tttcttaata tcccatcaaa tgaaatgtag cttgcatcat agtgcatccc
     4561 agctgaatgc gctcaatttc tttgtgactt ggttttctag ttttcttatg taagatttgt
     4621 ttcatgatgt tactcctttg ttctttatga gtctagttta catcaaaaaa agaccgcttg
     4681 ccataaccta accttacaaa agagacttta atcttacatt tttgtaagat tgggtgaaat
     4741 ataagcaaaa ttacttagaa aatgatttga tttcctattt ttaaagttgt aaaatataag
     4801 tatgaaaagg cttactttaa aaaggaggaa gcgggaacta acattttagt aaggttacct
     4861 agataacatg ccattcaatt taacacttgc aagtcgttca acctataagg aaagaaggtg
     4921 cgctttaatg aaacataaag aacatatact catcggattt ctctatctgc tctctccatt
     4981 catcggtcag ctcttagttg aacacacaca ctttatcagt acagaattta ccggcactgc
     5041 ttatgttatt tgctggttat cagttgttat cagcatccac catttttcca aaaatgtatt
     5101 gtctcaacag caaaaataga ttaaaaaact gcgtttaaat tactgctttt atagtaaaat
     5161 gaaataagaa caggacaaat cgatcaggac agtcaaatcg atttctaaca attttttaga
     5221 agtataggtg tcctattcta gttccaatcc actatagttt gtggatgaag ctggcgaaat
     5281 attattaata ctattcaaaa atcaaagtgc aaactatatc aacttcacat tgccgactaa
     5341 attacagcct gcggctggct tcctagtttg cactttgaat tttcggctct ataatatttg
     5401 tagtgggtaa cccccctatg gatattatgg agccta
//
LOCUS       DAVLSL010000123         5360 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLSL010000123 DAVLSL010000000
VERSION     DAVLSL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.125"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(91..1482)
                     /locus_tag="VXN30_001698"
     CDS             complement(91..1482)
                     /locus_tag="VXN30_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1358291.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKMEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEAAILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(1717..2094)
                     /locus_tag="VXN30_001699"
                     /pseudo
     CDS             complement(1717..2094)
                     /locus_tag="VXN30_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            2327..3304
                     /locus_tag="VXN30_001700"
     CDS             2327..3304
                     /locus_tag="VXN30_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1358292.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFRKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            3301..4383
                     /locus_tag="VXN30_001701"
     CDS             3301..4383
                     /locus_tag="VXN30_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1358293.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     SEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(4447..5360)
                     /locus_tag="VXN30_001702"
                     /pseudo
     CDS             complement(4447..5360)
                     /locus_tag="VXN30_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1616 a         1162 c          937 g         1645 t
ORIGIN      
        1 atcaattata gcataaaaca ttttttaagt agtactatct tttgtaaaaa ataagtctat
       61 cacatatctg acagacctat tttttcttgc ttattcatca cgactataat acagctcatg
      121 agcttttagg cgagtattga gcatctctgg tatggtcaca aaggtataac cttgattttt
      181 caaatactca atgacccttg gcaaggcatt gactgtcgga ctgtgaatat catgcatcaa
      241 aacgatagag ccgttagcta cttgatgctg aatttccgtc aaaatagctg cttcattttt
      301 actcttccag tccagactat ccacatccca cataataaag ctcaaatcga ggctattgcg
      361 aatatcatct gtaatagctc cataaggcgg acgcatgagt ttggaactag aacccagcac
      421 tttagttagc acatcctcag tatcagtaat ctgcttttta gcttcatcaa gagatagttg
      481 cgagagaata ggatggctcc aactatggtt tccaacaaca tgaccttcag attttatcct
      541 cttcaccaag tcctcattcc cagaaacatt tttcccaagc acaaagaaag tcgctttaat
      601 atcatattta gctagggtct ctaatacctg cggggtcgtt gctggatttg gaccatcatc
      661 aaaggttaga gcgacaactt tttgatgttt cttatcaaag taagattggt acaaggccgc
      721 atctttttcg agtaagtacg aagattggat aacatcaaag aaagcagata ctggcaaggc
      781 tatctcttct aaattttcaa ccacaggact tggataaagg ataatctgac tatccttgta
      841 atcaaaattc catgcagaca agtcttggtc agagaagttt tttacaatct gctcactctg
      901 gtcttgctcc atttttttat cctctatgaa ggaggtcaac tcttttatca gctgttcctt
      961 agccttacta gcatctgaaa acagttggtc aagtgtaaaa ggttgcccat cttctgttaa
     1021 gtgaaccttt cctagactgg tcttttcagt ctcttcaatc ttaaaagcaa ctaaatcata
     1081 gatttgtttg gtcacattac gattaacgac tccctttaaa cctgactctt cttgctctgt
     1141 atagtagaaa acaagattgt ccttactttc tagcttgtcc ttaacatctt gatttatcag
     1201 ctccctaaca gaggaaatca ctttctcccc ttggagagga taataggcaa tcacttcggc
     1261 ttgcccctga cgaaaatgct ccttctgatt tccctcactc aattgatcat ctttctcttt
     1321 tttgagcgat tcaatctttt gctcaaaact tttttgctgg tagatcttaa aagcaataaa
     1381 tccgcctaat aggcaaatag aaattgctaa aataccaatc aaagccaata atacatgtct
     1441 cgttttcccg tgtctgccac gtcctagtct acttttattc atatttatat cataacaaat
     1501 ttgagcaata atttcaatga tacaatttgg caatccacaa ttcctctagc aagccaaagc
     1561 atcttacatc aactccaaca tccgtatctg tagagattat gtagttttct atctatggaa
     1621 tttctagtag attgaagcta gaatagtaga cctatacttc taaaacattg ttagaaatcg
     1681 atttgactgt cctgatcgat ttgtcctgtt ctcatttcat tttactataa aactatgaaa
     1741 agctaggtca tctgagaagt aattactatc gacaattgcg atagcgtata ctagtgcgat
     1801 ttgtttgtag ctctggtggg tatcaccttc acgttggcga attttttcta ggttttgatt
     1861 aacctaactg cacagataag cccataggcg attgatgaaa aaattctgat gatgaacctg
     1921 aatttcgatg ataacttgga ttccattatc taactccgcc aagacgtcta tactagtata
     1981 gaaatcctgc gctgagtagg gcatggaagg caagacgtga atgttacttc cctccaaaat
     2041 cgtcacattt tttgctggca agtccagcat atcgcggata aattgacaaa tcattagttg
     2101 ggctgatacc cggatgtctg agaatcctcc ttttcctcct tctattatag cacattttta
     2161 aatctaggtt tgctagattc tatgcttcta tctatttatt cggaaaagaa agcttttctt
     2221 cagaagaaag ctaatagctt atttgaaatt ttctcctctc tgttggatag tcccgtctat
     2281 ctatgttata atgaaagaag gatttaaaat cggaaaagga gtatttatgc taaaattagg
     2341 tgtcatcgga actggcgcta tcagccatca tttcatagaa gcagcccata ccagtggaga
     2401 ataccaactg gtcgcaatct attctagaaa actagaaact gcagcaacct ttgcttctcg
     2461 ctatcagaat atccaactat tcgatcaatt agaagtcttc ttcaaatctt cttttgattt
     2521 agtctatatc gccagtccaa actccttaca ttttgctcag gcaaaagctg ccttgtctgc
     2581 tggtaaacat gttattctcg aaaagccagc tgtcactcaa ccacaagaat ggtttgattt
     2641 gattcaaaca gctgaaaaaa ataactgttt tatctttgaa gcagctcgta attaccacga
     2701 gaaagccttt actactatca aaaacttttt agcagataag caagtgttag gagcagattt
     2761 caactatgcc aaatactctt ccaagatgcc tgacttgttg actggacaaa caccaaatgt
     2821 cttttcagac cgttttgctg gtggagccct catggatttg gggatttatc ccctctatgc
     2881 tgctgttcgt ctttttagaa aagccaatga cgcgacttac catgctcaac agcttgacaa
     2941 tagcattgac ctaaatggag atggtatcct cttctaccca gactatcaag ttcacatcaa
     3001 ggctggaaaa aacatcactt ccaatcttcc ttgcgagatt tatacaacag atggaacctt
     3061 gactctcaac acgattgagc atattcgctc agctattttt accgaccacc aaggaaatca
     3121 agtccagctt cctatccaac aggctcctca tacgatgact gaggaagtcg ctgcatttgc
     3181 acacatgatc cagcaaccag atctgaatct ctaccaaact tggctgtatg atgcaggatc
     3241 tgttcatgag ctactatata ccatgcgcca gactgctggc attagatttg aggcagaaaa
     3301 atgaaaacca aactaccgac tgaatggcaa gaactgagtg accaactcgg tttccaagaa
     3361 ttcaccccca ttcaaactca actatttgag ccccttcttg ctggagaaaa cctcctagga
     3421 gtgagccaaa ccggaactgg taagacccta gcgtatctcc taccaagtct tctcagacta
     3481 caaaagaaaa aagcccaaca actcttgatt ctagcaccaa atacggaact tgctggacag
     3541 atttttgatg tatgtaaaac gtgggcagaa gctatcggat tgactgctca actcttcctt
     3601 tcaggttcaa gtcagaaacg acaaattgaa cgccttaaaa aggggccaga aattctgatt
     3661 ggaactcctg gccgtatctt tgaactcatt aaacttaaaa aaatcaagat gatgaatgtg
     3721 gaaaccatca tcctggatga atttgaccaa ttactagatg attctcagat tcactttgta
     3781 gaaaaaatca ctcactacgc acctcgtgac caccaactgg tctacatgag tgccacgacc
     3841 aagtttgacc aagaaaagat tgtgccgaat acacgcacta ttgatctctc tgaccaaaaa
     3901 ttggacaata tccagcattt ctacatgcag gtagaccaac gtcatcgagt ggatatgcta
     3961 cgaaaactgg ctcatgtaga ggatttccgt ggtctagtct tcttcaatag cctgtcagac
     4021 cttgggaatg ccgaggaaaa actacagtat cgtgatatat tgactgtttc cctcgctagt
     4081 gatgttaatg ttaaatttag aaaaatcatc ttagaaaagt ttaaagataa ccaactaacc
     4141 ctgcttcttg caactgacct tttggctcgt ggaattgata tcgatagcct agaatgtgtc
     4201 gtaaactttg atattcctag agatagcgaa acctacacac accgtgctgg ccgtactggt
     4261 cgtatgggca aggaaggata tgttatcact ctcgtcactc attcagaaga aatcaaaaaa
     4321 cttaaaaagt ttgcaagtat ccgagaaatt gtcctaaaaa accaagaact ctatatcaaa
     4381 taaagcccca ttgctctata cacatccagt gtatggagct tttttataag caattcaatt
     4441 ttatactcat ctgctttcaa aaagcattct agtccatctc cgattaacga tgggctttat
     4501 cacctccttc tccagtcctt gtatgatatc ttgaagttga ttcatgacat cttccaaagt
     4561 ttgaaaggct ttattcttaa atccacgttt acgaatctct ttcaacactt gttcaatggg
     4621 gttcatctct ggtgtgtatg gaggaataaa ggtaaaacca atattagtcg gaatctttaa
     4681 ggtacttgat ttatgccata tagcattgtc cataacgaat aaaagataat catctggata
     4741 agcttgtgaa agctcttcta aaaagacgtt catccactca gtattacatc caccagctat
     4801 taagaaaaat gattcgcctg tatgggcatc aacagctcca taacaatagc gaaattctcg
     4861 tatatagtga ctatggacat gtggacctac tcctattgga gaccaacaag atcccagttt
     4921 actgattcta ccgaaaccag cctcatcttg ggacatcaag tgaaccttac gaaaacgtct
     4981 actggtttta aatcgctttc ttttcttctt gaattgagat tttattttta gacgcgacaa
     5041 tggtttgagc gtctgctttc ttaggatgtt ctggacgtgg cgtaatattt cgccaaccat
     5101 ggcgcttcaa caattgatag aaggcatcac gtgtgtagga acgacctaac tcctttttat
     5161 aagcctgaaa taaggcatca attgtaacaa attctcctgc ctctgcagcc ttcaaatggc
     5221 gggcaagaaa gactttcttt tcctcaactg tcatatatgc atggttacga ccaccacgtg
     5281 tttcttgaag gagagagtcg agtccgaact cctcatattt ttttacattt tgctaaatcg
     5341 ttgtttgatt acagtctaaa
//
LOCUS       DAVLSL010000124         5307 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLSL010000124 DAVLSL010000000
VERSION     DAVLSL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.126"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..647
                     /locus_tag="VXN30_001703"
     CDS             <1..647
                     /locus_tag="VXN30_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1358294.1"
                     /translation="KIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRYRELPF
                     IDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEIFPLLD
                     EEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHISIGYL
                     RIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKFK"
     gene            672..1739
                     /locus_tag="VXN30_001704"
     CDS             672..1739
                     /locus_tag="VXN30_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1358295.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            1843..2853
                     /locus_tag="VXN30_001705"
     CDS             1843..2853
                     /locus_tag="VXN30_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358296.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2865..4556
                     /locus_tag="VXN30_001706"
     CDS             2865..4556
                     /locus_tag="VXN30_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1358297.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLLTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4559..5269
                     /locus_tag="VXN30_001707"
     CDS             4559..5269
                     /locus_tag="VXN30_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1358298.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1731 a          756 c         1024 g         1796 t
ORIGIN      
        1 caaaaattaa gttcaaagag aatggggaat ttaatcattt ccctgggaat acagttgtag
       61 caaatcttta tactaaacaa gatttgatgg aagttgttga tattattcaa tcacgttata
      121 gagaattacc atttatcgat aagtttactt taactccaag gaattctatt cacatgactg
      181 taattgaatt gttgtgccat gaaaatcgtg aaacggagtt ttggagcagt aatcttcctc
      241 tagatacacc tttacaggaa atacatgatt actttgctaa acaacttgaa atttttccat
      301 tgttggatga agaaattcat atgcgtataa ctgaaatggg aaaacaaaac atactagttg
      361 aacctgcaga tgaagcttct gcaaagagat tagaagaaat tcgtacttat gtctcagaaa
      421 aagcaggtgt ttgtttccct aatcatgata gatatcaatt ccatatttca attgggtatc
      481 ttcggattcc tctaaccgaa gaggaggaag aagagtttac taaagtcaga gcagaattaa
      541 ctgaaatttt attagagaag attccaacta ttactgtaaa ccgtattgac tatactgtat
      601 ttgaagatat gagacaattt gttccatatc acgaaaaatt taaataaaat aataaaaaag
      661 gagaacttat catgaaaaaa atgaaagttt ggtctactgt acttgcaacg ggagttgctc
      721 ttactacact tgctgcttgc tctggaggtt caaattctac gactgcttct tcatctgaag
      781 aaaaagctga taaaagtcaa gaattagtta tctattcgaa ctcagtctca aatggtcgtg
      841 gtgattggtt aactgctaaa gcaaaagaag ctggttttaa tataaaaatg gttgatatcg
      901 ctggcgctca attagcagac cgtgttattg ctgagaagaa taatgcagtt gcagatatgg
      961 tatttggaat tggtgctgtt gactcaaata aaattagaga tcaaaaatta ctagtacagt
     1021 acaagcctaa atggttagat aaaattgatc aatctttatc agataaagat aattattata
     1081 atcctgtgat tgttcaacca ttagttttaa ttggggcgcc tgatgtaaaa gaaatgccta
     1141 aagattggac tgaattaggt agtaagtata aaggtaaata ttcaatttat ggtcttcaag
     1201 gtggtacagg acgggcaatt ctagcaagta tcttagttcg ttaccttgat gaaaaaggag
     1261 atttaggcgt atcagaaaaa ggatgggaag ttgcaaaaga atactttgca aatgcctata
     1321 ctcttcaaaa aggtgaaagc tcagttgtta aagcgttaga taaagaaagc ccaattcaat
     1381 acggtatgat ctggggttct ggagcgttaa tcggacaaaa agaacaaaat gttgaattca
     1441 aagttatgac tcctgagatt ggtgtaccat ttgtaactga acaaactatg gttttaagca
     1501 ctagtaaaaa acaagcgtta gctaaagaat ttattgattg gtttggtcaa tcagaaattc
     1561 aagtagaata tagtaaaaaa tttggctcta tccctgccaa taaagatgcc ctaaaagagt
     1621 tacctgaaga tacgaaaaaa tttgttgatc aagtgaaacc acaaaatatt gactgggaag
     1681 ctgttggaaa gcatttggat gaatgggtag aaaaagctga attagaatac gtacaataag
     1741 aaataagagt tattttttat gtccaagtct ctctattatt tctaaattta aggaagaaat
     1801 aaagagaggc ttttggtttt agtaatagag ggagttagtg agatgattaa atttgataat
     1861 attcaaatta aatatggtga ttttgttgca attgataatc tgaatttaga tatacatgaa
     1921 ggggaattct ttacatttct tgggccttca ggatgtggta aatcaactac tttgagagca
     1981 ttggtaggtt ttttagatcc atcatcagga agtattgaag ttaatggaac agatgtcact
     2041 catttggaac ctgaaaagcg tggaattggt attgtatttc aatcttatgc gctatttcca
     2101 actatgactg tttttgataa tattgcattt ggtttaaaag ttaagaaggt agctccagat
     2161 gttattaaag ctaaagtatc agcagtggca gcaaaaatta agatctctga tcaacagtta
     2221 cagcgtaatg tatcagaatt atctgggggt caacaacaaa gggtagcatt ggctcgtgct
     2281 ctggttcttg aacctaaaat tctttgtcta gatgaaccat tgtcaaacct tgacgcaaaa
     2341 ttacgtgtag atttgagaaa agagttgaaa agacttcaaa aagagttagg tattactact
     2401 ttatatgtta ctcatgatca agaggaagcc ttgactttat ctgatagaat tgcagtcttt
     2461 aacaatggat acatcgaaca ggtcggtaca ccagtagaga tttatcataa ttctcaaact
     2521 gaatttgtat gtgattttat tggagatatt aatgttttga ccgatgaaac agtccacgaa
     2581 gtattattga aaaatacaag cgttttctta gaggataaaa aaggatacat tcgattagag
     2641 aaagttcgat tcaatcgtga aactgaacaa gattttattc taaaagggac aattattgat
     2701 gttgagtttt ctggagttac aattcactat acaataaaag tttctgaaag tcagattctt
     2761 aatgtaacaa gtattgatag tcaggctgct attagatctg tcggagaaag tgtggaatta
     2821 tttatcacac catcagacgt tctgcaattt taaggaggtg cagtatgcgt cataaattaa
     2881 atttaaaaga ttggcttatt cgtttaggat taatctggtt cttagtaaca tttattattt
     2941 atccaaactt tgatctagta gtgaatgtat ttgtaaaagg aggagaattt tcccttgatg
     3001 ctgtacatcg tgttctaaaa tctcagaggg cacttcagag tattatgaac agttttaagt
     3061 tagcattttc actcattatt acagttaatg tcgtaggtat tctttgtgtt ctatttacag
     3121 agtactttga tattaaaggt gctaaaattt taaaattagg ttatatgacc tctttaattt
     3181 atggaggagt ggttttagcg actggatata aatttgtcta tggtccttat ggattgatta
     3241 caaaattttt acaaaatgtt atcccttctt tagactctaa ctggtttatt gggtatggtg
     3301 cagtcttatt cattatgaca ttttcaggaa ctgctaatca tacattgttt ttaacaaata
     3361 caattcgaag cgttgactat cacactattg aggctgctcg aaatatggga gcaaaaccat
     3421 ttactgtttt ccgaaaagta gtgttactaa ccttaattcc aactctattt gcacttacta
     3481 ttatggtttt tcttagtggt ttatctgcag tagcagcacc catgattgtt ggtggtaaag
     3541 aatttcaaac tataaatcca atgattatta catttgcagg gatggggaat tctcgtgatt
     3601 tagctgcctt acttgcaatt attttaggta ttgcaactac aattttgctt actatcatga
     3661 ataagataga aaaaggtgga aattatattt ctatctctaa gactaaagcg cctcttaaaa
     3721 aacaaaaaat tgcgtctaag ccttggaata tcattgctca cattgtagca tatggattgt
     3781 tcacagtttt catgcttcca ctaattttta tagtattata ctcatttaca gatccagttg
     3841 caattcaaac aggtaactta acattatcaa actttacttt agaaaattat cgcttattct
     3901 ttagtaatag tgcggcattc tctccattct tggtcagctt tatttattct attattgctg
     3961 cgacaacagc aacaattctc gcagttgtat ttgctcgtgt tgtcagaaaa cataaatctc
     4021 gttttgattt cttatttgaa tatggtgctc tacttccttg gttactacca agtacacttt
     4081 tagcagtaag tttattattt acttttaatc agccacaatt tcttgtcttg aatcagattt
     4141 tggtaggtag tttggtaatt ctacttattg catatatagt tgtaaaaatc ccattttctt
     4201 atagaatggt acgtgctatt ttatttagtg ttgatgatga gatggaagat gcagcaagaa
     4261 gtatgggtgc ttcacctttt tatactatga tgaaggttat cattccattt attttaccgg
     4321 ttgttctctc tgttattgct ttaaacttta actctttatt aactgacttc gacttatctg
     4381 tattccttta ccatccccta gctcaaccat taggtattac gattcgatct gcaggtgatg
     4441 aaacagcaac atctaatgca caagctctgg tatttgttta tacaattgtt ctgatgatta
     4501 tttctggaac ggtattatac ttcacacaaa gaccggggcg taaagtaagg aaataatcat
     4561 gacagccact agtcttgggt tatcaaatat tgaaatagtt gtcaggattg ttttatcagt
     4621 agtcattggt agtataattg gtttagagag agggagcaaa tcccagcctg caggcatccg
     4681 aacttatagt attgtttgtc tagctgcatg tttgattatg atgacgaatg aatacgtatc
     4741 ttataaattt gggacaggag atcctacacg attaggagct caagttatat caggtgtggg
     4801 ttttctaggc gctggaacga ttcttattac agataaaaag aaaattacag gtctgacaac
     4861 tgcagcaggc atttgggctt cggcaggaat tggattagct attggagtag gtttttatga
     4921 gggagctctt ttagtagcca tttctgtttg gggtgtgata tccatgttcc aaccactaaa
     4981 aaaatatctg caaaatcgtt ctaaaatgat tgaattgtat atagtggtta aatctacaga
     5041 agcctataat cgtgtattgg tttattgtgc agaaaatggt atcagaatga ccgattcaag
     5101 aacagcgttt ggagatgtta attctgacag aattgaatac tttgatgttc cggacaaaaa
     5161 aatagcgtca tttattactc tggaattatc aggtagattt gaacatcttc gactgatgga
     5221 agaaattgct aatattgtag gtgttattta tgtcgaagaa attagctgaa atttagtaaa
     5281 caaagggaca gatgtttttg tcctttt
//
LOCUS       DAVLSL010000125         5225 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLSL010000125 DAVLSL010000000
VERSION     DAVLSL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.127"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            561..1013
                     /locus_tag="VXN30_001708"
     CDS             561..1013
                     /locus_tag="VXN30_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1358299.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGDFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1032..2369
                     /locus_tag="VXN30_001709"
     CDS             1032..2369
                     /locus_tag="VXN30_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1358300.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2448..3332
                     /locus_tag="VXN30_001710"
     CDS             2448..3332
                     /locus_tag="VXN30_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1358301.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3332..4165
                     /locus_tag="VXN30_001711"
     CDS             3332..4165
                     /locus_tag="VXN30_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1358302.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4183..>5225
                     /gene="nanB"
                     /locus_tag="VXN30_001712"
     CDS             4183..>5225
                     /gene="nanB"
                     /locus_tag="VXN30_001712"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1358303.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPALIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGDSQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPA"
BASE COUNT         1729 a          815 c         1033 g         1648 t
ORIGIN      
        1 taacaaaact aatttagaga atgaaatata gagtatttct ctcttaaaag tttttggtga
       61 aacgagatgt agaaaggaga tttagccaaa gagtctatta gtgctagaat aatagattag
      121 aattatttta gaaaaacgaa gtgagcagct tataaattca agtccccaaa tagattcata
      181 ctagtatctt ttgcaaaaaa taaagggcga cttccttcat gaatatcaat ttcatctata
      241 aggaaggtag ctaattgaac taacttattt attctgtttg tcgctagaaa aatcagacct
      301 ccttgtgaag attgaggaga tactgaatga aaatcaaaga agaaactagc aagctagcag
      361 cagattgcct aaaacaccgc tttgaggttg tagataagac tgacgtatat aatccaaggt
      421 gaagcgactg tggtttgaag agattttcaa agagtatagg ctagagagta gtgtttttat
      481 gtccttctag tagaaaatgc tagacagaag aatggggaac ttggatagga aaaatagatt
      541 gagaaaggag gttagaagag atgattatta caaaaattag ccgtttagga acttatgtgg
      601 gagtcaatcc acattttgca acattaatag attttctaga aaaaacagga ctagaaaatt
      661 taacagaagg ttcgattgct atcgacggta atcgattgtt tgggaattgc tttacttatc
      721 tagcagatgg tcaagcaggg gatttctttg aaacacacca aaaatatttg gatattcatt
      781 tagttttgga aaacgaagaa gccatggctg ttacatcgcc ggaaaatgta agcgttaccc
      841 aagaatatga tgaagagaaa gatattgaat tatacacagg gaaagtggaa cagttggttc
      901 atttgagagc tggcgaatgc ctcatcactt ttccagaaga tttacatcaa cccaaggttc
      961 gtataaatga tgaacctgtg aaaaaagttg tctttaaagt tgcgatttct taatgtagaa
     1021 agagaagaac gatgaaaaaa atgagaaagt ttttatgtct agctggaatt gcgctagcgg
     1081 ctgttgcctt ggtagcttgt tcaggaaaaa aagaagctac aactagtact gaaccaccaa
     1141 cagaattatc tggtgagatt acaatgtggc actcctttac tcaaggaccc cgtttagaaa
     1201 gtattcaaaa atcagcagat gctttcatgc aaaagcatcc aaaaacgaaa atcaagattg
     1261 aaacattttc ttggaatgac ttctatacta aatggactac aggtttagca aatggaaatg
     1321 tgccagatat cagtacagct cttcctaacc aagtaatgga aatggtcaac tcagatgctt
     1381 tggtgccgct aaatgattct atcaagcgta ttggacaaga taaatttaac gaaactgcct
     1441 taaatgaagc aaaaatcgga gatgattact actctgttcc tctttattca catgcacaag
     1501 tcatgtgggt tagaacagat ttgttaaaag aacataatat tgaggttcct aaaacttggg
     1561 atcaactcta tgaagcttct aaaaaattga aagaagctgg agtttatggc ttgtctgttc
     1621 cgtttggaac aaatgactta atggcaacac gtttcttgaa cttctacgta cgtagtggtg
     1681 gaggaagcct cttaacaaaa gatcttaaag cagacttgac aagccaactt gctcaagatg
     1741 gtattaaata ctgggttaaa ttgtataaag aaatctcacc tcaagattct ttgaatttta
     1801 atgtccttca acaagctacc ttgttctatc aaggaaaaac agcatttgac tttaactctg
     1861 gcttccatat cggaggaatt aatgccaaca gtcctcaatt gattgattcg attgatgctt
     1921 atcctattcc aaaaatcaaa gagtctgata aagaccaagg aattgaaacc tcaaacattc
     1981 caatggttgt ttggaaaaat tcaaaacatc cagaagttgc taaagcattc ttagaagcac
     2041 tttataatga agaagactac gttaaattcc ttgattcaac tccagtaggt atgttgccaa
     2101 ctattaaggg gattagcgat tctgcagcct ataaagaaaa tgaaactcgt aagaagttta
     2161 aacatgctga agaagtaatt actgaagctg ttaaaaaagg tactgctatt ggttatgaaa
     2221 atggaccaag tgtacaagct ggtatgttga ctaaccaaca cattattgaa caaatgttcc
     2281 aagatatcat tacaaatgga acagatccta tgaaagcagc aaaagaagca gaaaaacaat
     2341 taaacgattt atttgaggct gttcagtaga tgtaaaagac tagaaaatag gtgggctagt
     2401 gagctgaaaa gctctagccc aattttgtaa aagaagggag aaggagaatg gttaaagaac
     2461 gtaatttaac tcgctggata tttgttttgc cagctatgat tatcgtagga ttactctttg
     2521 tttatccgtt tttctcgagt attttttata gctttaccaa taagcatttg attatgccta
     2581 attataaatt tgttggtttg gctaactata aagctgtgct atcagatccc aacttcttta
     2641 atgcgttctt taattcaatt aagtggacca ttttctcatt agttggtcaa gttttagtag
     2701 ggtttgtatt ggctttagct cttcacagag tacgccactt caagaaatta tataggacct
     2761 tattgattgt tccttgggca tttcctacca tcgttattgc cttctcttgg cagtggattc
     2821 taaacggggt ttatggctac ttacctaatc taatcgtaaa attaggttta atggaacata
     2881 cacctgcatt tttgacagat agtacatggg cattcctatg tttggtgttt atcaacattt
     2941 ggtttggagc accaatgatt atggttaatg tgctttcagc tttgcaaaca gtaccagaag
     3001 aacaatttga ggctgctaag atagatggtg cttcaagttg gcaggtgttc aagtttatcg
     3061 tctttccaca tattaaagtg gttgtaggac ttctagttgt tttgagaact gtatggatct
     3121 ttaataactt tgacattatc tacctcatta ctggaggtgg accagccaat gctacaacga
     3181 cgcttccaat ttttgcttac aacctgggtt ggggaactaa attgttgggt cgtgcttcag
     3241 cagttacagt attgctcttt atcttcttgg tggcgatttg ctttatctac tttgctatca
     3301 tcagtaagtg ggaaaaggag ggtagaaaat aatgaagaag aaatccagta tttatttaga
     3361 tattctctca catgtacttt tagttggtgc gaccatcgtt gcaattttcc cattggtatg
     3421 gattatcata tcttctgtca aagggaaagg ggaattaact cagtatccaa cacgattttg
     3481 gcctgaacag tttacattag attatttcac tcatgttatc aacgatttgc acttcattga
     3541 taacattcga aacagtttaa tcattgcctt ggctacaacc ctcattgcga ttattatttc
     3601 tgctatggca gcctatggta ttgttcgatt ctttcctaaa ttgggagcaa tcatgtcgag
     3661 actactcgtc attacctaca ttttcccacc aattttgtta gcaattccct attcaattgc
     3721 cattgctaaa gttgggttaa caaatagttt atttggcttg atgatggttt atctatcttt
     3781 tagtgttcca tatgcagttt ggctcttagt tggatttttc caaacagttc caattggaat
     3841 tgaagaagcg gctagaattg atggtgcaaa taaatttgtt acgttttata aagttgtgct
     3901 accgattgta gcaccaggta ttgtagcaac agctatttat acatttatca atgcttggaa
     3961 tgaattcctg tatgccttga ttttgattaa caatacagga aagatgacag tagcagtagc
     4021 ccttcgttca cttaatggtt cagaaatact agactgggga gatatgatgg cagcgtctgt
     4081 tattgtagtt cttccatcaa ttattttctt ctctatcatc caaaataaga ttgcaagtgg
     4141 attatcagaa ggatctgtga agtagacgaa agaaggaaaa aaatgaataa aagaggtctt
     4201 tattcaaaac taggaatttc tgttgtaggc attagtcttt taatgggagt ccccgctttg
     4261 attcatgcga atgaattaaa ctatggtcaa ctgtccatat ctcctatttt tcaaggaggt
     4321 tcatatcaac tgaacaataa gagtatagat atcagccctt tgttattaga taaattgtct
     4381 ggagatagtc agacagtagt aatgaaattt aaagcagata aaccaaactc tcttcaagct
     4441 ttgtttggcc tatctaatag taaagcaggc tttaaaaata attacttttc aattttcatg
     4501 agagattctg gtgagatagg tgtagaaata agagacgccc aaaagggaat aaattattta
     4561 ttttctagac cagcttcatt atggggaaag cataaaggac aggcagttga aaatacacta
     4621 gtatttgtat ctgattctaa agataaaaca tacacaatgt atgttaatgg aatagaagtg
     4681 ttctctgaaa cagttgatac atttttgcca atttcaaata taaatggtat agataaggca
     4741 acactaggag ctgttaatcg tgaaggtaag gaacattacc tcgcaaaagg aagtattgat
     4801 gaaatcagtc tatttaacaa agcaattagt gatcaggaag tttcaaatat tcccttgtca
     4861 aatccatttc agttaatttt ccaatcagga gattctactc aagctaacta ttttagaata
     4921 ccgacactat atacattaag tagtggaaga gttctatcaa gtattgatgc acgttatggt
     4981 gggactcatg attctaaaag taagattaat attgccactt cttatagtga tgataatggg
     5041 aaaacgtgga gtgagccaat ttttgctatg aagtttaatg actatgagga gcagttagtt
     5101 tactggccac gagataataa attaaagaat agtcaaatta gtggaagtgc ttcattcata
     5161 gattcatcca ttgttgaaga taaaaaatct gggaaaacga tattactagc tgatgttatg
     5221 cctgc
//
LOCUS       DAVLSL010000126         5192 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLSL010000126 DAVLSL010000000
VERSION     DAVLSL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.128"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            107..633
                     /locus_tag="VXN30_001713"
                     /pseudo
     CDS             107..633
                     /locus_tag="VXN30_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390504.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(800..871)
                     /locus_tag="VXN30_001714"
     tRNA            complement(800..871)
                     /locus_tag="VXN30_001714"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(836..838),aa:Arg,seq:cct)
     gene            complement(914..1261)
                     /gene="rplS"
                     /locus_tag="VXN30_001715"
     CDS             complement(914..1261)
                     /gene="rplS"
                     /locus_tag="VXN30_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1358304.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1380..1709)
                     /gene="crcB"
                     /locus_tag="VXN30_001716"
     CDS             complement(1380..1709)
                     /gene="crcB"
                     /locus_tag="VXN30_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1358305.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1703..2077)
                     /gene="crcB"
                     /locus_tag="VXN30_001717"
     CDS             complement(1703..2077)
                     /gene="crcB"
                     /locus_tag="VXN30_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1358306.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGAGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(2074..2340)
                     /locus_tag="VXN30_001718"
     CDS             complement(2074..2340)
                     /locus_tag="VXN30_001718"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1358307.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVTYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2456..2899)
                     /locus_tag="VXN30_001719"
     CDS             complement(2456..2899)
                     /locus_tag="VXN30_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1358308.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            3003..3938
                     /locus_tag="VXN30_001720"
     CDS             3003..3938
                     /locus_tag="VXN30_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1358309.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3992..4276
                     /locus_tag="VXN30_001721"
     CDS             3992..4276
                     /locus_tag="VXN30_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1358310.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(4333..4551)
                     /locus_tag="VXN30_001722"
     CDS             complement(4333..4551)
                     /locus_tag="VXN30_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358311.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(4548..5133)
                     /locus_tag="VXN30_001723"
                     /pseudo
     CDS             complement(4548..5133)
                     /locus_tag="VXN30_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1669 a         1089 c          894 g         1540 t
ORIGIN      
        1 tgacaattta gatattccgt tcgaatttaa actaacaaaa gaagcacaaa atcttattat
       61 cgaacatagt gactatatca aagcatacta tgataaagac ataactatgg aagattattg
      121 taaagaaaaa acaatatctc ttttaaagca acttttggtg atataaaatg gagtctctta
      181 aataaacaag ctcataggaa tactgtatca ggagattttg gatgcttatc taacacacga
      241 aaggctcagg gaagacattt ggaacaagaa ggtaatatta aacatgcttt aatatattac
      301 atagaatctt tgataattac tatttcagga ttagaaaaca atttttcagc cactgattat
      361 ccagtatatt atcccgattc gatacctgac tactcactaa aacatattca aacattaatg
      421 gaatcattat ctgatgacga ttatgatttt gcttttgatg aagcattatt tcgcttctca
      481 attttgaatg caaatcattt tttatctaag gaagatattg actatttaag agttaattta
      541 cctcgttcca ctgctgaaga aataaacaat tacttaaaga aatatgaatg ttatagtcct
      601 ttaaataatt tagaacttga cgattttgaa taaattgact atacaaacat ttatatactc
      661 gatatagtct caattttatc tgatgattgc ccaaattttt caataataaa acgcataata
      721 ttatggagac aatcccctat attatgcgtt cttttaatat caaagacttt ttgacaaact
      781 tctttgatat ctaattacat gccccctgca ggaatcgaac ctgcaactac tccttaggag
      841 ggagttgtta tatccattga actaagggag ctagataaaa actctgctga aaaagcagag
      901 ttttttagtc gaattaacga cggatttctt tgatacgagc tgccttacct tgaagagcac
      961 gcaagtagta caatttcgca cgacgtactt taccgtaacg tacaacttcg attttttcaa
     1021 cacgtggagt gtggattggg aagatacgct caacacctac accgttagag attttacgaa
     1081 ctgtgtagtt ttcagagatg ccagcacctt tacgtgcaat aacaacacct tcaaaaatct
     1141 ggatacgttc acggttacct tcgacaactt tcgcatgtac acgaacagtg tcaccaggac
     1201 ggaatgatgg gatatctgta cgaagttgac cttcagtcaa gctttggatt aatggattca
     1261 ttttattctc ctatctttgt caatcttgag gaatcttcct cagcggataa actgtatttt
     1321 tgtgcgtcca ttacacacaa gatatagttt accaaatttc taaaaaaaag taaagaaatt
     1381 tatagcaaaa ttcctaaaaa aatcgcaacc aaaccaccta tgtaagtcaa agtcaggtaa
     1441 gaataaaaga ccttcttatc acttagcagt ctttgaagtt cgtcattcaa ggtcgaaaaa
     1501 gttgttaaac cgccacaaaa tcctgttgct agaatagcat atacttcctt agactctaca
     1561 tgattgtaga atactccaat taaaaaacaa cctaaaagat tagctatgag cgttccgagt
     1621 ggcaatttag aagcttgatt atagcgagaa aagaaatacc gtacgagtgc tcccaagcca
     1681 caggcgattg caagatagac gattaccatt tcttcctccc caaatagtaa gctaaaagca
     1741 ggcctccacc gatactcaaa agcaaataca ggactaaact aaaataacgc cctgtatcaa
     1801 gcagcttcac agtatcaagc attaggctgg aaaaagttgt taaacctccg caaaaacccg
     1861 cccccagtgc taaaatcaag cccttactag tccccttata gaccagatag cccttgacaa
     1921 gaaaaatcaa gcagaaaatt cccagatagt tgacaaagag agtgccccaa ggaaagtctg
     1981 gactggctgg taaccaggtg gaaactagat atcggacaag tccgcccaac atagcagcta
     2041 gaaaaattcc tagcggataa aattgttctt ttttcatttg atattttgat cctgataatc
     2101 acgcgaacgt ttgagtatgt ccgaaaaagt cgcgacaata gtctcctgat agcgcttgtc
     2161 ctctacacga ctcctaacct tttcaaaaat aacttcttct ctcttagtat ctaaaatcgg
     2221 tttacctgaa gctttcttat aagtaaccac cccctcaact aaatgcattc gttcttctaa
     2281 cagtttgaca atttggtcgt cgatttgatc gatttcttga cgaataatat ctaaatccat
     2341 acagtctcct cctttatttg aattattgta tcaaaaagcc atcaattagg ctagtaaaat
     2401 cccaactctc gataagaaaa atgcactgat tgattacatc agcgcacttt tatgtttatc
     2461 ctactttagc agccaattct tctgcgaatt gttccaagcg ttcaatatct tcttcctcgg
     2521 cagaaagatc aactttaaca cactctgaac ctttttctgc tcctgttgac acaaagacac
     2581 ggtcaaagtc atcaacagcc ttacagaatt catcgtagaa ggtatctcct gagccgacca
     2641 ctccgtagat tttgccattc aagttgagat ctgctaggtc ttcgtagaag tccatcatct
     2701 catctggcaa ttcaccatca ccataagtat aggtcgcaac gatagcgatg tctgcttcca
     2761 agaagtctga agcgtcaaca gttgtacatt catcaacatc aacatccaag cccaagtcac
     2821 gtaatttgtc tgctacaata tctgcaattt cttcggtatt accggtcata ctggcaaata
     2881 caatttttgc taatgccata tcgtcctcct caatttatct ttctccatta tatcacatct
     2941 ttttcatttt aaaaataaaa attcttgtgc tacaatgaaa tgagaaagaa ttgaggttat
     3001 ttatggagat ttgccaacaa attttagaaa aaattaaaga atacgacacc attatcattc
     3061 atcgtcatat gaaaccagac cctgatgcct tgggaagtca ggtgggattg aaagccttgc
     3121 tagaacatca tttcccagaa aaaaccatca aagccgtcgg ttttgatgaa ccaactctta
     3181 cttggatggc tgagatggat cttgttgaag atagagccta ccaaggcgca cttgtcatcg
     3241 tctgtgatac agctaatact gctcgtatcg atgataagcg ctatagtcaa ggtgattttc
     3301 tcattaagat tgaccaccat ccaaatgatg atgtatacgg tgacctgtct tgggtcgata
     3361 ctagttcaag tagcgctagc gagatgatta ccctatttgc ccaaacaacc caactagcct
     3421 tggcagatcg cgatgctgag ttgctctttg caggaattgt cggtgataca ggtcgcttcc
     3481 tctacccttc taccactgca cggactcttc gcctggctgc ttatttgaga gaacataact
     3541 ttgactttgc ggctctcact cgcaaaatgg acactatgag ctacaaaatt gctaaactgc
     3601 aaggctacat ctacgaccat ctggaagtgg atgaaaatgg tgctgctcgc gttatcctga
     3661 gtcagaaaat cttgaaacaa tacaatataa ccgatgctga aactgcggcc attgtaggtg
     3721 cacctggacg cattgacaga gtgagtctct ggggaatttt tgtcgaacag gctgatggcc
     3781 actaccgagt tcgcttacgc agtaaagtcc atcctatcaa tgaaattgcc aaggagcatg
     3841 atggtggagg ccaccctcta gcaagtggtg ctaattccta tagcctagaa gaaaacgaaa
     3901 tcatctacca aaagttaaaa aacttgctta aaaactgata aaatacttgc caaacttttc
     3961 agaatctgat agactagtat agtaacaatc tatggctcgc aaagagacca tggcagaaag
     4021 gaaatattgc aaaatgaaaa aagatatcca tccagaatat cgcccagttg tcttcatgga
     4081 cacaactact ggttaccaat tccttagcgg ttcaacaaaa cgctctaacg aaacagttga
     4141 gttcgaaggc gaaacttacc cattgatccg tgtggaaatt tcatcagact cacacccatt
     4201 ctacactgga cgtcaaaagt tcactcaagc agatggacgc gtggatcgtt tcaacaaaaa
     4261 atacggtctc aaataatgat aagagaacag ttttggctgt tcttttttgt ttcttgaaat
     4321 caactgctgt tttcatgttc cagactcatc tgtaggttcg atttccatgc tactaggcag
     4381 gaaggaaata gctgtttcaa cacgtccata atgagctata ctattgtcac gaaccacact
     4441 ttcattgatg gtccaagtgg aattcatttt cttaaaagct tctcggactt tttccaaatc
     4501 tttggaggca atggcctgct ctaaggtttc aaaacgagga cttatactca tctgctttca
     4561 aaaagcattc tagtccatct ccgattaccg atggacttta tcacctcctt ctccagtcct
     4621 tgtatgacat cttgaagttg attcatgaca tcttccaaag ttcgaaaggc tttattctta
     4681 aatccacgtt tacgaatctc tttccacact tgttcaatgg gttcatctct ggtgtgtatg
     4741 gaggaataaa ggtaaaatca atattagtcg gaatatttaa ggtacttgat ttatgccata
     4801 tagcattgtc cataacgagt aaaagataat catctggata agcttgtgaa agctcttcta
     4861 aaaaggcgtt catccacact cctttttata aacctgaaat aaggcatcaa ttgtaacaaa
     4921 ttctcctgcc tctgtagcct tcaaatgacg ggcaagaaag gctttctctt cctcaactgt
     4981 catatatgca tggttacgac caccacgtgt ttcttgaagg agagagtcga gtccgaactc
     5041 ctcatatttt tttacgtttc gccaaatcgt tgtttgatta cagtctaaaa gctctataat
     5101 ctctttataa gatttgccca tcagacgaaa tatagtagat tgaaactaga atagtacacc
     5161 tctacttcta aaacattgtt agaaatcgat tt
//
LOCUS       DAVLSL010000127         5054 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLSL010000127 DAVLSL010000000
VERSION     DAVLSL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.129"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            136..279
                     /locus_tag="VXN30_001724"
     CDS             136..279
                     /locus_tag="VXN30_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV1358312.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            246..461
                     /locus_tag="VXN30_001725"
     CDS             246..461
                     /locus_tag="VXN30_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1358313.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPLVWAALKQIKVTLKKDDEL"
     gene            448..981
                     /locus_tag="VXN30_001726"
     CDS             448..981
                     /locus_tag="VXN30_001726"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1358314.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVMDLL
                     REVPTIFECLECFFKTR"
     gene            1339..1593
                     /locus_tag="VXN30_001727"
     CDS             1339..1593
                     /locus_tag="VXN30_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358315.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            1704..2783
                     /locus_tag="VXN30_001728"
     CDS             1704..2783
                     /locus_tag="VXN30_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1358316.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEELAE
                     LISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNVTDKKLSSMT
                     TTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKEGRQEKL
                     AESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAYQKTVQQ
                     LTITTVRMLRKQWFPKQQPLLRRRL"
     gene            2741..3262
                     /locus_tag="VXN30_001729"
     CDS             2741..3262
                     /locus_tag="VXN30_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1358317.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            3485..4354
                     /locus_tag="VXN30_001730"
     CDS             3485..4354
                     /locus_tag="VXN30_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1358318.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
BASE COUNT         1708 a          875 c         1055 g         1416 t
ORIGIN      
        1 caggtcagat tgaattttgg ggttatatga tgttgaggct aggaaaaatt caatttcagt
       61 aaaaaaagta agtcttctca taacaaaaca ttgatatagt tgcttagttt taaacaagca
      121 tattataata aagctatggc atatagtact gattttaaac agcgagcatt agattacatc
      181 aaagaggggc acagccatgt cgaggcagca aagttttttg gtgttggcgt cagaactctc
      241 ttcacgtggg aaaagaaaga cgtgaacaag gacacataga gaggaaaaag cgagtcgtca
      301 aaaaccgaaa gattccttta gaggaattga aagcctttgt agaggctcat ccagatgctt
      361 ttttacggga aattgcggca cattttgatt gtgctgttcc tttagtatgg gcagctttaa
      421 agcagattaa ggtcacttta aaaaaagatg acgagcttta aggaacaaga gccagagaaa
      481 gtcgctgagt ttcttgatat tttggataac ctaaaagatt taccagtcct atatattgat
      541 gaaacaggaa tcgaccgcta cctctatcgt ccttatgcag gggctcctag aggggagaaa
      601 gtctatgaaa agattagcgg acgtcgtttt gagcgaactt caattgttgc aggacaagta
      661 gacggagagt ttatagctcc catgatttac aagaaaagca tgacaagcga tttctttgtg
      721 gagtggttca aaacgcaact cctacctgct ttgaagacac ctcatgttat tgtcatgggc
      781 aatgctagtt ttcatcccaa gaacattttg gatgaactct gcatccaaga taaacacttt
      841 ttcttacctc taccacctta ttcaccggat ttgaatccta ttgagcaagc ttgggctatc
      901 ttgaaaaaga aagtgatgga tttattaagg gaagttccaa ctatttttga atgtttggaa
      961 tgctttttta aaactagatg actataacgg ttctaaagga acctatcgag tagtcattaa
     1021 aactaaggat actgctggtt aagagaagac ggtatacgat caaaccattc accgtgtagc
     1081 cgaaatcgtt cagaatgaag acttgtatca gaatgaagac ttgtataaga aaggtttgaa
     1141 tgttgaactt gcgcaccaac aaattaaggg attttttgaa gcagagttta aaaatcgtat
     1201 taatggagtt cttaatacta aaataaaaaa tagtacatta aatcgtgtaa ataaaaaaac
     1261 tatacaccag agcaacaaaa actccatgat caatttgaag cagaagcaac ggaagatact
     1321 aaaaaacaag gcgatattgt gttgaatgtt gaccaggatt tcatgagcat atctaagtct
     1381 aataaaagtg gttcagactg gaagaaaact ttcacagtga ggataaccaa taggctagca
     1441 aatgacttga ataatgtctt gaaacaggtt gataaagata ctcctaatac cccaacttgg
     1501 ctaaactcag ctgcttctaa agctaaagat gatgacagag tatataaact actgaagact
     1561 cttataccag gagaaaatta cctatcatgt taaggataat cagctagaag tagaaacaga
     1621 taaatacaca tatactgccg ctagaaatgg tagtaaggaa gttggtattc aagagtcaga
     1681 tatagcagca actctaagtg ccgatgaata taattctaat cgccaaacct ttgagagaga
     1741 atacaaatac aaaagcaaat gcccttaata atggttgggc tagatctggt tctgaagagt
     1801 tcaaaaagtt ctcccacttt gtaggggtag acaaagggat tgtgcgaacg aatgtactga
     1861 ctggtaaaaa actatctgat aagattagga aagaagtggg ctctggagat agcaaactag
     1921 gaaaaggcgg ctatttctct actggggatg ttctattagg aaaagatgtt gtttcttata
     1981 ccgtacaagt attttcagag aataatgaaa gagtaggagt aaacactcaa agttaccgtg
     2041 ttcagtataa tctcccaatt ctagctgact tttcagtcat ccaagatact gtggaaccat
     2101 cacgaaccgt tgttgaaaaa atcattccaa aactaaatat tcccgaagaa gagaaaggga
     2161 aaataaccga agaattggca gaactaatct cagaaaatgt gaaagttcgc tatgttgatg
     2221 aacaagggcg tttgctatca ttgaaaaatg atactggaat tggagaaaaa gaaagtgacg
     2281 gaacctacat taccaataaa aaacaactga ttggtaccag ctataatgtc acagataaaa
     2341 aactcagtag catgactact actgacggaa aatattatac ttttaaagaa gcagatacaa
     2401 attctgcaag tttaactggg aatattgtaa gcgaaggtag aacagtgacc ttagtttata
     2461 gagaaagcga agcgccaacc actgctacag taacagccaa ttactataaa gaaggtaggc
     2521 aagagaagtt ggcagagtct gttataaaag ctgatttagc gataggttct gagtatacca
     2581 cagaatcaaa aactattgaa gggaaaacaa caactgagga caaagaagac cgagttatca
     2641 caaggaaaac aacatacacc ttggtagcaa ctcctgcaaa tgcgtaccag aagacggtgc
     2701 aacagttgac tattactacc gtgagaatgt tgaggaaaca gtggttccca aaacagcaac
     2761 ctctactgag acgaagacta taacgcgtat cattcattac gttgataaag ttacgaacca
     2821 aaatgtaaaa gaagatgttg ttcaacctgt aaccttaagc cgtacaaaaa ctgagaacaa
     2881 ggtcacggga gttgtaacct acggtgaatg gacaacagga aactgggacg aggttatatc
     2941 tggtaagatt gacaagtaca aagatccaga tattccaaca gttgaatcac aagaagttac
     3001 gtcagactct agtgataaag aaataacggt aaggtatgac cgtttatcaa caccagataa
     3061 accaactcca gaaccaggta ctccaaaaac tgaaactcca gtgaatccag acccagaagt
     3121 tccgacttat gagacaggta agagagagga attgccaaac acaggtacag aagctaatgc
     3181 taccttggct agtgctggta tcatgacctt gttagctggt ctaggattag gattcttcaa
     3241 gaaaaaagaa gatgaaaaat aatagatttt agaatctagg aacaaggaaa agatcacaga
     3301 tgtgggcttt ttcctggttt tgagaatgag gtctttcgtg aagaataaaa acgcttacaa
     3361 gtacgttgaa ctgggaaact atgaatccta tttttttaaa aatatttcca gaaatcagtt
     3421 gctaaatagg gtaaaaattg ataaaataag gaatatctaa aaaattttaa ggagaatcta
     3481 gcaaatggat ttcacatggg cactgaagta tgccactgaa tttttgggaa ctgccatttt
     3541 gatcattctt gggaatggtg cagttgccaa cgttgaactt aaaggtacga aaggtcacca
     3601 aagtggctgg atcgtcatcg ctgttggtta tggtatgggg gttatgatcc cagccttgat
     3661 gtttggtaac gtatctggga atcacatcaa ccctgctttc actctagggc ttgcagttag
     3721 tggtcttttc ccttgggcac aagtggtacc ttacattatc gcgcaagtct tgggggctat
     3781 ctttggccaa gccttagttg tggcaacata ccgtccattc tacttgaaaa ctgaaaaacc
     3841 aaataacatc ttgggaactt tctcaactat ttcaagtatt gaccatggta caaaagaaag
     3901 tcgctatgca gcaactgtca atggtttgat taatgagttt gttggttcat ttgttttgtt
     3961 ctttgcagct cttggtttga ctaaaaactt ctttggtgct gaagtgcttc aattcatgaa
     4021 acaaaaggca atagaagcag gacaaacagt tgatttttct gacttggcta ttaaagcaca
     4081 ggtggctcca cacactgctt caggactttc tgtggctcac ttggcacttg gattcctcgt
     4141 tatggctttg gtaacatcac ttggaggacc tacaggacct gccttgaacc cagcccgtga
     4201 cttgggacca cgtctccttc atgctttcct tccaaaatca gttcttggtg agcataaagg
     4261 cgattcaaaa tggtggtatt cttgggtacc agtagtagca cctatcgcag cagcaattgc
     4321 ggcagtagct gtattcaaat tcctttatct ctaagaaata gctcctttaa catttgagtg
     4381 agcaccatct ataagtaaga gaggatcaga ctggttctct ctttttgatt ttttagggaa
     4441 atgaaagaaa ctctaaacaa actcctctcc agcagtggtt tagaagtctc agtgggctat
     4501 tccagcttca atgggctata gtaggttgca gttgaaataa tagacccttg tttctaaaac
     4561 attgttagaa attggtttga attctccaat caaattgtgc agttttcatt ctactatata
     4621 ttatcggaga tgggttccct atcttgtaag tctgctttat agtgggttga agttggaata
     4681 gtcctccctt ctttctcaaa cattgtgagg aattgattta ccttcctcaa caaaatgttc
     4741 agtttctatt tcattttgct ataaaataag cgattagggg ggctattctt cgacctacat
     4801 tgactctgct gagtcctatg attgttatcg ttttatctgc aattttatac tcaatgaaaa
     4861 tcaaagggca aactaagaag ctagccgcag gttgttcaaa acacagtttt gaggttgtat
     4921 agtagattga aactagaata gtacacctca acttctaaaa cattgttaga aatcgatttg
     4981 attgtcctga tcgatttgcc ctattcttgt ttcattttac tatataaacc agagaatgtt
     5041 tacattttca gcaa
//
LOCUS       DAVLSL010000128         4985 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLSL010000128 DAVLSL010000000
VERSION     DAVLSL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.130"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..1195
                     /locus_tag="VXN30_001731"
     CDS             <1..1195
                     /locus_tag="VXN30_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358319.1"
                     /translation="AGADGRTSYFHIAYAASADGSREFSLEDNRQQYMGYYSDFTAAD
                     SRDRTKYKWFDRLANVQVGSQNLLRNTATLPIKNGLDGTWRSTSGGNGVAEPVTLDKY
                     PVPGILKGVRVKNNTNGGNKDLSQIINLVIGQRYTISCWARVSSTSDRSTVNLLVRSW
                     TVNDTNRILFKAISNKTWVKYSLSFTADAEKNSIQFGQNGPGNIEICGMKLELGNVAT
                     DWSLSVEDIQSQLDGKADQKLTQQQLTALTEKAQLHDAELKAKATMEQLSDLEKAYNA
                     FVKSNADSRKKSESDLVEAGRRIDLLTTQFGGLAELKTFIDTYMKSTNEGLIIGKNDA
                     SSTIKVSSDRISMFSAGKEVMYISQGVINIDNGIFTASIQIGRFRTEQYHLNKDVNVI
                     RYIGG"
     gene            1206..3284
                     /locus_tag="VXN30_001732"
     CDS             1206..3284
                     /locus_tag="VXN30_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="structural protein"
                     /protein_id="HEV1358320.1"
                     /translation="MTKFINSSGSLHLNIYIEQVSQDIANNSSRVSWKATVDRDGAYR
                     TYTYGNISNLSVWLNGSSVHSSHPNFDTSGQEFTLASGEVTIPHSGDGTKTFAVWASF
                     DPNNGVHGNITVSANYTLSSIPRSSSVSDNALSGNRRLGSPHTLTIDRKSSSFTHQVW
                     YRVFGSNWIDLGKNHATGVSFVPNIDLARYNTKAKSGTMDICVRTYNGTTQVGNDVYS
                     NGWYFEIPESVKPTFSGITLTDMNTVARQLLSGNNFLQIISDIQVNFNNPSGAYGSTI
                     TGYRAEIVNKNQVTTVNGGRLGMMNFNGSATIRASVVDSRGRQSDTRDITINVIEYFA
                     PAFSFTAFRTRETPNIIQVVRNAKIAPITLSGSQKNVMTLSFKVARLGSTTFTADHGR
                     ASGIWTTQHTLNNSAANMAGNYVATKSFVVIGTLSDKFTSTEFTATVATESVVMSYDK
                     DGRVGIGKVAEQGGAGSLDVLGDIYARNKPIQQYQLTDNNGCGKLIKQDFNTMKETGT
                     WWINGSSQNNPFSGTWGMLEVFRPNPGSHERIQRFTTSTGYMAVRENGFDNNWRPWRY
                     LVQQSKSTNNSDYVALLKSESTPTPWQNAILQNGWNHHRDYGGVQFSKTFDGVVCFKG
                     TCKGGKIARESIILTLPEHFRPSTTLFKTALNNDYGSAVIGIYPNGNVVVKSNVDATW
                     LNFDNVFFKI"
     gene            3344..3607
                     /locus_tag="VXN30_001733"
     CDS             3344..3607
                     /locus_tag="VXN30_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358321.1"
                     /translation="MLKVTKTRQLVTEFFAQDGDQQKLVKTTVINTDNKAVSTISETL
                     HDPELYANNRISMRKHEQELREMRYKIEDAILAELEADSEHKE"
     gene            3617..4033
                     /locus_tag="VXN30_001734"
     CDS             3617..4033
                     /locus_tag="VXN30_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV1358322.1"
                     /translation="MKIEFFNFLRSVVQTEDGLVLYALTLIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFAFLYSICLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            4037..4369
                     /locus_tag="VXN30_001735"
     CDS             4037..4369
                     /locus_tag="VXN30_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1358323.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            4373..>4985
                     /locus_tag="VXN30_001736"
     CDS             4373..>4985
                     /locus_tag="VXN30_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1358324.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHWR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAETYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKING"
BASE COUNT         1635 a          903 c         1096 g         1351 t
ORIGIN      
        1 ggctggtgca gatggtagga catcctattt ccatatagca tacgcagcaa gtgcagacgg
       61 atcacgcgaa tttagtttag aggataatcg ccagcaatat atgggctatt attccgattt
      121 taccgcagca gatagtagag atcgaactaa gtataaatgg tttgaccgac tagctaatgt
      181 tcaagtgggt tcccagaact tgcttagaaa tactgcaact cttcctatta aaaatggatt
      241 agacggtacc tggcggagta cgtcaggtgg taacggagta gcggaacctg ttaccttgga
      301 taaatatcct gtacctggaa tcctaaaagg tgttcgagtt aaaaacaaca ccaacggggg
      361 taataaggac cttagccaga ttattaactt agttataggt caacgttata caatatcctg
      421 ctgggctcgt gtaagctcta caagtgatcg atctactgtg aacctactag taaggtcctg
      481 gacagtgaac gatactaata ggatactttt taaggctata agtaataaga cttgggttaa
      541 gtacagtctt tccttcactg cggacgctga gaaaaattca attcagttcg gtcaaaacgg
      601 accaggtaat attgaaatct gtggaatgaa attagaactc ggtaatgtag ccactgattg
      661 gtccttatcc gtagaagaca ttcaatctca gttagacgga aaagctgacc aaaagctaac
      721 tcaacaacaa ttgacggccc taactgaaaa ggctcagtta cacgacgcag agttgaaagc
      781 taaggctacg atggaacaat taagtgattt agaaaaagca tataatgcct ttgtgaaatc
      841 aaatgcagat agtcgaaaaa aatctgagtc tgatttagtt gaagcaggta gaagaattga
      901 tttgctgacg acacaatttg gaggattagc agagcttaaa acattcattg atacttacat
      961 gaaaagcaca aatgagggct tgattatagg taagaatgat gcaagctcta ctattaaggt
     1021 atcaagtgat agaatatcca tgttttctgc aggtaaggaa gttatgtaca tttcgcaagg
     1081 tgtaataaat attgataatg gtatttttac tgcatcaatt caaattggac gttttagaac
     1141 agaacagtat catcttaaca aagatgtgaa tgtcatacga tatataggag gttaaaaaga
     1201 gggaaatgac taaatttatc aattctagcg gttcactaca cttgaatatt tacatcgaac
     1261 aagttagtca agatatcgct aacaattcct caagagttag ttggaaagct actgttgacc
     1321 gtgatggagc ttaccgcaca tatacttatg gtaatattag taacttgtct gtatggttaa
     1381 atgggtcaag tgtgcatagt agtcatccaa actttgacac atccgggcaa gagtttactt
     1441 tagcaagtgg ggaagtaacc atcccacaca gtggtgacgg aactaagact tttgcagtat
     1501 gggcatcgtt tgacccaaac aacggagtac atggaaacat taccgtatca gcaaactata
     1561 ctctttcaag cattcctcga tctagtagtg taagcgacaa tgctctttca ggaaataggc
     1621 ggctcggaag tccccacact ctcactattg atcgcaaatc tagctcattt actcaccaag
     1681 tatggtatag agtgtttggt agcaactgga ttgatttagg aaaaaatcac gcaacaggcg
     1741 tttctttcgt acctaatatt gacctagcta gatacaacac aaaagcaaag tctggcacga
     1801 tggacatatg tgttcgaaca tataatggaa ctactcaagt tggaaatgac gtttactcaa
     1861 atgggtggta ttttgaaatt ccggaaagcg taaaacctac attttctgga attacattga
     1921 ctgatatgaa tactgttgct aggcagctat tgagtggaaa taacttttta cagattattt
     1981 ctgatattca ggtcaacttt aataatccat ccggggctta tggttcgact atcacaggat
     2041 atcgtgctga aatcgtaaac aagaatcagg ttacaactgt aaacggtggt aggcttggta
     2101 tgatgaattt caatggttca gcaacgattc gagctagtgt ggttgatagc cgaggcaggc
     2161 aatcagatac tagagatatt acaatcaatg ttattgaata ttttgcacca gcttttagtt
     2221 ttacagcttt taggacgcgg gaaacaccta acattattca agttgtcagg aatgctaaaa
     2281 tcgctcctat cactttatca ggtagtcaaa aaaatgtcat gacactatca ttcaaagtag
     2341 ctcgattagg tagtacaact tttacagctg atcatggtag agcttctggt atttggacaa
     2401 ctcaacacac cttaaataat tcagctgcta acatggcagg taattatgtt gcaaccaaat
     2461 catttgtggt cataggaact ctatctgata agtttacaag cacagaattt acagcaacag
     2521 ttgcaactga aagtgtggta atgagttatg acaaagatgg ccgtgtgggc attggtaaag
     2581 ttgcagagca aggtggtgca gggtcattgg atgtcttggg agatatctac gctagaaata
     2641 aacctattca acaatatcaa ttgactgaca ataatggatg tggaaaactt atcaaacagg
     2701 attttaatac gatgaaagag actggaacgt ggtggataaa tggtagctct caaaacaacc
     2761 ccttttctgg aacttggggg atgctagagg tattcagacc taatccaggc tctcatgaac
     2821 gtattcaacg cttcactacc tcaacaggat atatggcagt tagggagaat ggttttgata
     2881 acaactggag accatggcgc tatctggtgc aacaatccaa gtctactaat aactctgact
     2941 atgtagcttt gctaaaatca gaaagcactc cgactccttg gcaaaatgcc attttacaaa
     3001 atggatggaa tcatcacagg gattacggag gtgtccaatt ttcaaaaaca ttcgatggtg
     3061 ttgtttgttt taaaggaaca tgtaagggcg gaaagattgc acgtgagtca atcatactta
     3121 ctttacctga acatttcaga ccatctacaa cattattcaa aactgctttg aataatgatt
     3181 acggttcagc cgttatagga atttatccaa atggaaacgt agttgtcaaa tctaatgtag
     3241 atgctacttg gcttaatttt gataatgtgt ttttcaaaat ataacaaccg taaaaaatcc
     3301 ctaattatta acggataatt aatttataaa ggaggaaatg acaatgttaa aagtcactaa
     3361 aacacgtcag ctagtaactg aatttttcgc acaagatggc gaccaacaaa aattggtcaa
     3421 aactactgta atcaacacag acaataaagc tgtttcaaca atatctgaaa cgctgcatga
     3481 cccggaactg tacgctaaca atcgtatcag tatgcgtaag catgagcaag agttacgaga
     3541 aatgcgctat aagattgaag atgccatttt ggcagagctg gaagcagatt ctgagcataa
     3601 ggaataggag gtgtgtatga aaattgaatt tttcaatttt ctaagaagtg tcgtacagac
     3661 tgaagatggt ttggtcttgt acgctctaac actgattgtc tcaatggaaa tcattgattt
     3721 tgtgacaggg acgattgcgg cgattatcaa tcctgacatc gagtacaaga gcaaaatcgg
     3781 cattaacggg ctccttcgta agatttcagg ggttctctta ctgatgatcc tcattccggc
     3841 gtccgttttg ttgcctgaaa agacaggttt tgcattcttg tactcaatct gtctcgggta
     3901 catcgcattt acttttcaat ctctcattga aaattaccgc aaattaaaag gaaatgttac
     3961 tctttttcag ccgattgtaa aagtatttca gcgattactt gaaaaagatg atgatacgaa
     4021 aaaaggagaa taacaaatgc aacaaattac tgaaatcatt actaatggag caatcagcat
     4081 cctagtcgtt ttggcagggg ttgtagttag ggcagtcaag gactacctgg ttcaaaaagg
     4141 tggagaaaag accatcaaaa tcgttgaaat cttggccaaa aatgcagtaa atgccgtgga
     4201 gcaggtagct gctgaaactg gctacaaggg agatgaaaaa ctggcacagg ctcgcgctaa
     4261 agtccgtgct gagcttacaa aatacaatat tagcatgact gacaaagact tagacacctt
     4321 cgtagagtca gcagtgaagc agatgaatga cgcatggaaa ggacgatagg gaatggatat
     4381 cgatagaaac agactacgta caggcttgcc acaggttggg gtgcagcctt atcgacaagt
     4441 acatgctcac tcaacaggta accgcaactc aaccgtacag aatgaagcgg attatcactg
     4501 gcggaaagac ccagaattag gttttttctc gcacgttgtt gggaacggtc gcatcatgca
     4561 ggtaggacct gtgaacaacg gaagttggga tgttgggggc ggttggaatg ctgagaccta
     4621 tgcagcggtt gaactgattg aaagccattc aactaaggaa gagtttatgg ctgactatcg
     4681 cctctatatc gaattgctac gcaatctagc ggacgaagca ggcttgccga agactcttga
     4741 tacagacgac ttggcaggta tcaagacgca tgaatactgt accaataacc aaccaaacaa
     4801 ccactcagac catgtggatc catatccata tcttgcaagt tggggcatta gccgtgaaca
     4861 gtttaagcaa gacatcgaaa acggcttgag cgctgcaaca ggctggcaga aaaatggcac
     4921 tggctactgg tacgtacact cagacggctc ttatccaaaa gataagtttg agaaaatcaa
     4981 cggta
//
LOCUS       DAVLSL010000129         4883 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLSL010000129 DAVLSL010000000
VERSION     DAVLSL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.131"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            73..669
                     /locus_tag="VXN30_001737"
                     /pseudo
     CDS             73..669
                     /locus_tag="VXN30_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            745..1194
                     /locus_tag="VXN30_001738"
                     /pseudo
     CDS             745..1194
                     /locus_tag="VXN30_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1207..2298
                     /locus_tag="VXN30_001739"
     CDS             1207..2298
                     /locus_tag="VXN30_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358325.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            2288..3923
                     /locus_tag="VXN30_001740"
                     /pseudo
     CDS             2288..3923
                     /locus_tag="VXN30_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4026..4718)
                     /locus_tag="VXN30_001741"
     CDS             complement(4026..4718)
                     /locus_tag="VXN30_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1358326.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1528 a         1042 c          840 g         1473 t
ORIGIN      
        1 tagaattgta ggttttaaaa acgcttacat tgttttaaaa taaacttaaa taacacaacg
       61 gaggtatcca tcatgaaaaa gaaatggatg tattatgctg cttgttcttc taatgaatct
      121 gccgatgaca gttcatctga taaaggagac ggtggttcgc tagtcgttta ttgaccaaac
      181 tcagaaggct taattggagc aactattcct gcctttgaag aaaaatatgg tatcaaaata
      241 gaactgattc aagctggtac tggagaactt ttcaaaaaac tagagtcaga aaaagaagtt
      301 cctgtagctg atgttatctt tggtggttct tatacacaat atgctaccca cggagaactc
      361 tttgaaaact atatttcaaa agaaaatgat aatgttatca aagaatatca aaacacaact
      421 ggcttctcta ctccttatac actagatggt aatgttttaa tcgtccaccc tgatttaact
      481 aaaggcatga acatcgaagg atatagcgac cttctcaaac ctgaactaaa aggaaaaatc
      541 gcaactgctg acccagcaaa ctcttctagc gcctttgctc aattaacaaa tatgctacaa
      601 gctcaaggtg gttacaaaga tgataaggct tggtcttatg taaaagatct tttcacactt
      661 attgatggta tagtaaaatg aaataagaac aggacaaatt aatcaggaca gtcaaatcga
      721 tttctaacaa tgttttagaa gtagatgtgt actattctag tttcaatata ctataaaatc
      781 ggttcaagtt catctggtgt ctataaagca gtcgctgatg gagaaatgac tgttggtctc
      841 tcttatgaag atccagcagt taaactctta aatgacggag ctaacattaa ggtagtctat
      901 ccaaaagaag gaaccgtctt cctaccttct agtgctgcta tcgttaaaaa atctaaaaat
      961 atggaaaatg ccaagaaatt tatcgatttt attatctctc aagaagtaca agatacactt
     1021 ggtacaacca ctactaaccg tcctgttcgt aaaaatgcta aaacaagcga aaacatgaaa
     1081 ccaattgaca aaatcaaaac actcacttaa gattatgatt atgtcatcaa gaataaatca
     1141 gatatcgtta agaaatacaa cgaagtcttt acagatatcc aatctaaaca gtaaaagagg
     1201 ttcactatga gtgagatcga aattattaac gccaaaaaaa tctaccacga tgtccctgtt
     1261 attgagaatt tgaacattac aattccaaaa ggaagtctct ttacccttct tggagcttca
     1321 ggatgtggga aaacgaccct tcttcgtatg attgcaggtt tcaacagtat caaagatgga
     1381 gaattttact tcgatgatac aaaaatcaat aatatggaac ccagcaaacg caatatcggg
     1441 ctggttttcc aaaactacgc tattttccca catttgactg tccgagacaa cgttgctttt
     1501 ggtcttatgc aaaagaaggt tccaaaagaa gaattgattc aacagaccaa caagtatctt
     1561 gaactcatgc aaattgctca atatgcggat cgaaagcccg ataaactcag tggtggacaa
     1621 caacaacgtg tcaccttggc atgcgcctta gcggttaatc caagtgttct cctcatggac
     1681 gagccactta gtaatctgga ggccaaactt cgcttggata tgcgtcaaac catccgagaa
     1741 atccaacacg aagtgggaat tacaactgtt tatgtaaccc acgaccaaga agaagccatg
     1801 gctatttcag accaaattgc tgttatgaaa gatggggtga tccaacaaat cggccgacca
     1861 aaagaactct atcataaacc agctaatgag tttgtggcaa cctttatcgg acgcacaaat
     1921 attatccctg ccaatcttga aaaacggagc gacggcgctt atatcgtctt ttcagatggc
     1981 tatgcccttc gaatgctagc tcttgatcag gttgaggagc aagctattca tgtaagcatt
     2041 cgtcccgaag agtttatcaa agatgaatct ggagatatcg aaggaactat tagcgatagc
     2101 gtctatcttg gactaaatac ggagtatttc attgagacag gttttgcctc aaaaattcaa
     2161 gttagtgaag aatcaacttt tgaagaagat ctacaaaaag gcaatcgtat tcgtctacga
     2221 atcaatacgc aaaaattaaa catcttttct gcagatggtt cccaaaacct gataaaagga
     2281 gtcaaccatg gaatgtaaaa aactaaatat ttggacagcc tcctctttct tcctctttct
     2341 tacctatctt gtctttctcg tttatcctat cgttaccgtg ctcaagcaag cacttataca
     2401 tgaaggacaa ttctcactag ctaattttgt cactttcttt agtaaagcct actactctga
     2461 gacactagtc aacagtttca gggtttccat taccgctact gtcacttcct tagttgtagg
     2521 aaccctatta gcttatctct tctctatgta tgacttcaag gggaagaaat ttctacaaat
     2581 attgattatc attgcttcca tgtcagctcc tttcgtagga gcctactcct ggattctctt
     2641 gctgggacga aatgaggtca tcactaaatt tttgacaaat gccctttatc ttccagctat
     2701 cgatatttat ggattcaaag gaattgtact tgtctttaca ctgcaactat tcccactggt
     2761 atttctatat gttgctggaa caatgaacag tattgacaat tctctacttg aagcagctga
     2821 aagcatgggg tccttcggat ttaaacctat cgtaacggtt gttttacctc tcctagttcc
     2881 aaccttacta gcagctcctt gcttgtattt atgagagcat tctcagactt tggaacgcct
     2941 atgttgattg gcgaagggta tcggactttc cctgtcctga tttataccca atttattagc
     3001 gaggttggag gaaattctgc ttttgcaatt atggcgatta tcattgcctt ggcaattttc
     3061 cttatccaaa aacacattgc aaaccgctac agtttcagca tgaatctgct ccatccaatt
     3121 gagcctaaaa aaactacaaa aggaaaaatg gctgccattt atgcaacagt ctacggaatt
     3181 atctttatct ctgttttacc tcaaatctac ttaatttata cctctttcct aaaaacatca
     3241 ggtatggtat ttgttaaagg ttattctcca aacagttaca agctagcttt caatcgtatg
     3301 ggatctgcta ttttcaatac cattcgtatc cctttgattg ccttagttct agttgttcta
     3361 tttacgacat ttatctccta cctagccgtt agaaaacgga atttgtttac aaacttaatt
     3421 gacagcctca gtatggtacc ttatattgta ccaggaaccg ttctagggat tgccttcatt
     3481 tctctggtct atttggaagt ggatttctta tgattacagg gactgctttc atcttgatta
     3541 tgtctctatc tgccagaaaa ttaccttata ctattcgctc atctgttgct agcttacaac
     3601 aaatagcacc aagtattgaa gaagctgctg aaagcttagg aagtagtcgt ctcaatatct
     3661 ttgctaagat tacaactcca atgatgctat cggatattat ttctggagcc atcctatctt
     3721 gggtcacact gatttcagaa ctctctactt ctatcctcct ctacaatgtc aaaacaagaa
     3781 caatgactgt agctatttat acagaggttc tcagaggaaa ttacggtgta gctgcagcct
     3841 tgtcaactat cctgactgtt ctaacagtag gttccttgct cttgtttatg aaaatctcta
     3901 aaagcaatag cattacactt tagttttcca catcaaaaac agccgaaagg agtaccctcg
     3961 gctgtttttt cttatcttga tattcttatc aaatccctag ctcatggcaa gcaagtctag
     4021 actgattaaa tagaaccttc ttccatcttc tcacgtccta gctctcggta actacggtca
     4081 ccgacaactt caaccctaaa ctgaccgtcc tcatattcaa gaatcgtcac gctaccatta
     4141 tctagaccat gcggatgcat gccattaatc agataaacaa tggttccaat agtcattcca
     4201 tggctgacaa caagggcgtt acctccacct tgatcttcca tttcttttgc aattatttca
     4261 aagccttcct tgattcggcc actgagtttt tcccaacctt cagcccaacc agctgtatcg
     4321 acctctacca agccctcagc cagttcagca taagacaatt ggtgaacgtg gtccacatta
     4381 aagatacgag gaataatgcc catgaaaaga tcgccatcat aggctccatc aaaactaccg
     4441 aaacaccatt ctctgatacg cttgtccatg cgataaggga ttttcccctg caagccaagt
     4501 tcttcaagta taattcccat ggtctgaatg gtacgaccag aatcactcga ataagcgcgc
     4561 tcaaactgta gatcagattc tcgcaaaccg attcctaact cttgaatccc tcgttcacct
     4621 tcagcagtta agggagtatc gctccaacct tgcgcgcgac caatcgtgtt aaacatggtc
     4681 ttgccatgac gtaccaaata caatcgtact tttaccattt tccgtcctcc gtttgcttct
     4741 attatatcat gaatgataaa ataaaagcca cccatacagg cgacttttga aggagattat
     4801 tatgaaaaag tttaggagtt cattaaataa agatattaga tgaaaatcaa attcaaacta
     4861 attcagtgtt atctgtttca aat
//
LOCUS       DAVLSL010000130         4775 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLSL010000130 DAVLSL010000000
VERSION     DAVLSL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.132"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            38..742
                     /locus_tag="VXN30_001742"
     CDS             38..742
                     /locus_tag="VXN30_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1358327.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     misc_feature    801..888
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            924..1238
                     /gene="rplU"
                     /locus_tag="VXN30_001743"
     CDS             924..1238
                     /gene="rplU"
                     /locus_tag="VXN30_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1358328.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1254..1598
                     /locus_tag="VXN30_001744"
     CDS             1254..1598
                     /locus_tag="VXN30_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1358329.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            1615..1908
                     /gene="rpmA"
                     /locus_tag="VXN30_001745"
     CDS             1615..1908
                     /gene="rpmA"
                     /locus_tag="VXN30_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1358330.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2219..2424)
                     /locus_tag="VXN30_001746"
                     /pseudo
     CDS             complement(2219..2424)
                     /locus_tag="VXN30_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2861..3778
                     /locus_tag="VXN30_001747"
     CDS             2861..3778
                     /locus_tag="VXN30_001747"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1358331.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3871..4719)
                     /locus_tag="VXN30_001748"
     CDS             complement(3871..4719)
                     /locus_tag="VXN30_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1358332.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEVHEILGEG
                     FTFEKE"
BASE COUNT         1517 a          817 c          973 g         1468 t
ORIGIN      
        1 gaaagaaaat ggtacaatat ttctaagaga aaatacaatg ggaggtaaaa tgaggttatt
       61 acctataaga aaaatatcac gtcagtctaa aaggttagca ctttttttga cgttttgtgc
      121 tggatatgtg gatgcttaca cttttattgt tcgcgggaat acccttgtag ctggacaaac
      181 tggaaatgtt gtctttcttt cagtagaatt aattaaaaat aatgtttcgg atgttaggga
      241 caaggttctc accttgctag cgtttatgat gggagtcttt ttattaacga tttataagga
      301 aaaattgaga attgtgaaaa aacctattct gtccttgatt cccttggcaa tcttatcaat
      361 cattattgct tttgtgccgc aaactgtgga taatatctat ctagtgccgc ccttggcctt
      421 ctgtatggga ctggtgacaa ctgcttttgg agaagtgtcg ggtattgcct ataataacgc
      481 ttttatgaca gggaatatca aacggaccat gctggctttt ggagattatt tccgaaccaa
      541 gcacactcct tttttgcgtg aaggattcat atttgttagc ctgcttagta gttttgtcct
      601 tggcgttgtc ttttcagcct atttgacgat tttctatcat gaaaagacca ttcttggtgt
      661 tcctattatg atgagcgttt tttacctcag catgcttttt gcctcttggc agaaaaaagt
      721 aaaagaaaaa gcttcatttt aggtgttatt acttgtaaga aaataggaga tatgctatac
      781 ttgaagagtt gactatgcac gatcctgtgc aaccgcacga acatcgttgc ttgattattc
      841 aagtttaagt ggttcgtccc acgatgctag gcgagtcttc acaaaaaatc ggggaaaccc
      901 ataaaaatca taggaggtgc ataatgagca catacgcaat tatcaaaact ggcggaaaac
      961 aagttaaagt tgaagttggt caagcagttt acgttgaaaa attgaacgtt gaagctggtc
     1021 aagaagttac ttttaacgaa gttgttcttg ttggtggtga aaacactgtt gtcggaactc
     1081 cacttgttgc tggagctact gtagttggaa ctgttgaaaa acaaggaaaa caaaagaaag
     1141 tggttactta caagtacaaa cctaaaaaag gtagccaccg taaacaaggt caccgtcaac
     1201 catatacaaa agttgtcatc aacgcaatca acgcttaatt ttaaggagaa cacatgatac
     1261 aggcagtctt tgagagagcc gaagatggcg agctgaggag tgcggaaatt actggacacg
     1321 ccgagagtgg cgaatacggc ttagatgtcg tgtgtgcatc ggtttctacg cttgccatta
     1381 actttatcaa ttctattgag aaatttgcag gctatgaacc aatcctagaa ttaaacgaag
     1441 atgaaggtgg ctatctgatg gttgaaatac caaaagatct tccttcacac cagagagaaa
     1501 tgacccagtt attctttgaa tcatttttct taggtatggc aaacttatcg gagaactatt
     1561 ctgagttcgt ccaaaccaga gttatcacag aaaactaaca cggaggaaaa cattatgtta
     1621 aaaatgactc ttaacaactt gcaacttttc gcccacaaaa aaggtggagg ttctacatca
     1681 aacggacgtg attcacaagc aaaacgtctt ggagctaaag cagctgacgg acaaactgta
     1741 acaggtggat caatccttta ccgtcaacgt ggtacacaca tctatccagg tgtaaacgtt
     1801 ggtcgtggtg gagacgatac tttgttcgct aaagttgaag gcgtagtacg ctttgaacgt
     1861 aaaggacgcg ataaaaaaca agtttctgtt tacccaatcg caaaataaaa aggttcattg
     1921 aaccttttat cccgaacctt gaaatgtaaa ggtgaggaag ctagaaacag cttaaaataa
     1981 ggctttccga gttttcggag agcctgtttt tttattttga tacccatact ttttaatatg
     2041 tatagtggat tgatactctt cgaaaatcaa attcaaacca cgtcaatttc accttgccgt
     2101 actcaagtac agcctgcggc taacttccta gtttgcactt taattttcat tgagtatgaa
     2161 tctggaatag aacactgtta aaaattgata tgagtttcct actcgatttc ttcagatttt
     2221 atttcaattg actataagaa ctgctatctg gtttcaaata ttggcttgtc gtcccttcac
     2281 ttgtctgaat ggtttgagcc gttccttctg ataagggaaa agatattctt tagctgaaac
     2341 ggtctgagaa tccaagctaa cagatggaga gaaaaatagc ccagtctgaa gcacactaga
     2401 tgggagttgt tggaaagcag tcatctagtt tttctttgct gttttggctt ttttctttgt
     2461 catcagggaa agtgacttgc ttcaaatcag ggagtttagg aaattcttca aacatcttgc
     2521 ccaagttgtc tcattttgta aattttacag ccataggtgt acctcgattc taaattctac
     2581 ctttttggca ccaattagta atttaaagtt ctcaggagtt agttgcattg ctttttcata
     2641 cataacatag tatgacactt tatggttacc gaacaagtct aatcactcct tgcaaaattt
     2701 tgaagatgtc ctacaagttt ttgagaagaa tattgcaaag ttcatagtta agcaacgata
     2761 gaataaagtg ctttttgaaa acaggtacac ttcatgtgaa aaaaacaaca tttcagattt
     2821 tctgttccag aaatagctat cgctatgata taattatttt atgattatta ctattcctat
     2881 aaaaaaccaa aaagatattg gcacaccatc tgattcagtc gttgttctcg gctattttga
     2941 tggcatacat aaggggcatc aagaattatt tcgtgttgcc aacaaggctg cgagaaagga
     3001 tttattgcct atcgtcgtta tgacctttaa tgaatctcca aagatcgctt tagagcctta
     3061 tcatccagat ttgtttttgc atattttgaa ccctgctgaa cgtgaaagaa aattaaagcg
     3121 cgaaggtgta gaagaattat atctccttga ttttagtagt caattcgcta gtctcactgc
     3181 acaagaattc tttgcaactt atatcaaggc tatgaatgcc aaaattattg ttgcaggttt
     3241 tgattataca tttggttctg acaaaaaaac agcagaagat ttaaaggatt actttgatgg
     3301 agaagttatc attgttccac ctgtagaaga tgagaaagga aagattagtt caactcgtat
     3361 ccgtcaagct attttagatg gaaatgtgaa agaagcagga aaacttttgg gggcaccgct
     3421 tccatcaaga ggtatggtgg ttcatggtaa tgctcgtggt cgtacaattg gttatccgac
     3481 agcgaattta gtgcttttag accgtactta tatgccagca gatggcgttt atgtcgttga
     3541 tgttgagatt caaagacaga agtatcgtgc tatggctagt gtcgggaaaa atgtgacctt
     3601 tgatggagaa gaagcacgtt ttgaagtcaa tatttttgat tttaatcaag atatttatgg
     3661 ggaaaccgtc atggtttatt ggcttgatcg cattcgtgat atgaccaaat ttgactcagt
     3721 tgaccaatta gtggatcagt taaaggctga tgaagaagta actcggaatt ggtcttaaga
     3781 gcttgagtaa ataaaacaaa aaagaggttg tctgtaaccc aaaagataga tgatttagtc
     3841 taacttttga ggtcacgaca ttacctcttt ttattctttt tcaaaggtga agccttctcc
     3901 taggatttca tggacttctg taatagttat aaaggcttga ggatcgattc gatgaatcat
     3961 ttccttcgtt ttcacaattt catttcttcc gacaatacag tagatgattt tcaaattttc
     4021 tttactatag tagccttgac cagaaataaa agtaacacct cttccgaggt catcattaat
     4081 cgccttagca agttggtcag gacgttttgt gataatcata aagcctttgc cggcatatcc
     4141 tccttcacca atcaaatcaa taacacgaga aacaataaaa tcaaacaaaa gcgtgtagga
     4201 aaccaatctc aaatccttga agattaggag aatcaacatg agaatacaaa aatctaagat
     4261 aaagagcagt tttcctatgg atatatgagt gtatttgttg agaatacgag ctagaatatc
     4321 agttccgcca gttgtacctc cagcattaaa aataattcca aggccaattc ccaataggat
     4381 tcccgctata agggctgtga ttagtaaatc accttgaaga tcaatatgaa ggggaatatg
     4441 ctcaaaaaaa gctaaccagg cggacaaagc taaggttcct agtaaactag aatagaggga
     4501 tttggctcca aagatcttcc aagctaggat gaaaagggga atattaatca gcaggttcat
     4561 gagggaaaca gggattttaa aaagataaaa ggtgatgagg gtaatgcctg tcgcccctcc
     4621 ttcaaagaga tgatggggaa ctacaaaata agtcagtcca aaagcataaa tagcagcacc
     4681 tagtaaaatg gtaaaaatgg ggtaaatttt tttaatcata ggacacctct tttcttataa
     4741 gttgattata tcaaatttta cggaaaattt gattg
//
LOCUS       DAVLSL010000131         4767 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLSL010000131 DAVLSL010000000
VERSION     DAVLSL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.133"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..237
                     /locus_tag="VXN30_001749"
     CDS             <1..237
                     /locus_tag="VXN30_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358333.1"
                     /translation="SSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQY
                     MVNSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            247..945
                     /locus_tag="VXN30_001750"
     CDS             247..945
                     /locus_tag="VXN30_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358334.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            942..1589
                     /locus_tag="VXN30_001751"
     CDS             942..1589
                     /locus_tag="VXN30_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358335.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEISDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1669..2530)
                     /locus_tag="VXN30_001752"
                     /pseudo
     CDS             complement(1669..2530)
                     /locus_tag="VXN30_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(2542..3300)
                     /locus_tag="VXN30_001753"
     CDS             complement(2542..3300)
                     /locus_tag="VXN30_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1358336.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(3301..3978)
                     /locus_tag="VXN30_001754"
     CDS             complement(3301..3978)
                     /locus_tag="VXN30_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1358337.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(3938..4606)
                     /locus_tag="VXN30_001755"
     CDS             complement(3938..4606)
                     /locus_tag="VXN30_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1358338.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIDLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
BASE COUNT         1434 a          800 c         1018 g         1515 t
ORIGIN      
        1 agcagttttt cttggtttaa aaaatcaaac atgagcttag tatttctgtt tactttttta
       61 tttgtagaat ccttattctg gatttatcag ttggacaatg ggataattgg attattgcca
      121 atttttcagt atatggtaaa ttccaatccg tatgcattga tttattggct tacattacta
      181 tctatcataa ttccattgac tgtattttct gttcatagaa actggaggag agtgtaaaag
      241 ttggaaatgg gaaagttaag tagtcacatg tggaggttga atcagataat ctataccaag
      301 tacttttggg gttatgttct tttttggata ttgatttgtt taggattatg gtattggtta
      361 gaaggaaatg atagacttgt tatagaaatt ttaaaagggc ctaatctgag tcaaaactct
      421 tttttagtct tatctatatg gttgcttcat tggtttatta ttcatacatt ttttctagca
      481 gttgtatatc gtagaagagc atccgatttc tttatggaag tgattcgatt ttcttctatt
      541 aagctctgga ttaggtatca gatttggacc tgttttcttt atggactcat tttaatcatg
      601 gtaaaagttc tagtgattca atttatgtta cagttaccaa actgggatat aggagttttg
      661 tttatagttg attctttgaa tgcttgtgtg ttagtcttgt tttgctttat gttatacgca
      721 ctaggagcga atgtacaaat gaactttgct tgcgttagtt tctttttact catgattgtg
      781 tttggtggtc tatttgtagg aaatcgaact aattatctat tttatatttt gaatcgaggg
      841 aatggagata taggacgaga tttatttctt cagttgctat ttttagtctt tctttttaaa
      901 agtattttct atttcactcg tcaaaagagg aggtttatag aatgaatgaa ataatcatat
      961 taaaaaatat tgagttgaaa ttaaaaaaaa catgtgtttt tcaaaacctt aattttagtt
     1021 gtaaacaggg ggaaattata ggaattactg gtgcgaatgg ctcagggaaa agtgtattgt
     1081 ttaaattaat agctggttta tatagtccgt cttatggaga agtgttaatc aatggggaaa
     1141 atattgttcc tgagagaaaa attccagcta atttgggagc tttgattgaa gaacctggtt
     1201 ttataaatta ttatagtggc tttaagaatt tacaatattt ggcaagcata cgaggagtag
     1261 ttggtaatca ggaaatcagt gatacactga aaatagttgg tctatatgag caaaaagacc
     1321 agaaagttaa aacttattcg ctaggtatga ggaaaaagct agggattgct caagcaatta
     1381 tggagaatcc ctctattctt ttactagatg aacctatgaa tgccttggat aaatcaagtg
     1441 tagaaaatat gagaacattg tttagaaagc tctctagtga aaaagggaca acaattttga
     1501 ttgctagtca tagtgaagag gatattcgta tcttatgtga taaagtatat gcaatagaag
     1561 ataaagtatg tacactgtgt tcagattgaa catcttagat agactcagga tgttataggt
     1621 aaaatcttaa aaagggattg gagcttaaaa tccagtccct tttaagagtt aatggccacc
     1681 ttcaacaacc aggtcatctg ctttagcagc gtcaccgtag gttgggtgaa gtgttttttc
     1741 ataggccttg tggaagaagt tttccttgcc taatttttca atatctttat tgatgaagtc
     1801 tagcaattct tggttgcctt tttgaactgc tgccgcaatg gtatcgggat caccgaggga
     1861 agtaattcct acttcaaatc ctttattttc aagcgcccaa gctagaactt ccgtattgtc
     1921 agttgaaaag gcatctccac gtccgtcaag aagagcttgg taagagtcac tgtattggtc
     1981 gtatttttgg agtttgattt ctggatgatt cttttcaaaa taagtctcag cagtcgttcc
     2041 ttttgtgaca attaaggttt taccttcaag ttgtttgacg tctgtaatga gaccagtctt
     2101 aggtgatacg acacccagag aaactttcat atatggaagg gcaaaatcaa cttgtttctt
     2161 acgttcgtca gttactgtaa agttagcaag agtaatatct accttgtttg aaatcaagta
     2221 ttccgcacgg ttggcagcat cgactgaaat gtatttaacc ttgacaccaa ggtctttagc
     2281 tagttggttc cctagttcaa tatcgtagcc ttggtaagaa ccatcattgt caacgtagcc
     2341 aaacggtttt ttatctccaa acacggcgat tcgcagttca ccgccttttt ttgatttcat
     2401 cgatagtgcg agctttggca gttgtttttc cagaggatga accagcgttt ccacctgagc
     2461 tacaagcagt gataaagata agggcaaagg caagtgctaa aacagttaag agtggtttga
     2521 atagtttcat aagaatctcc tttatagata tgagccaaat tggctaaagt caaagacgtt
     2581 taaaaattcc tgggctcgtt tggtttgcgg attggtaaag aaggcttgag ctgttccttc
     2641 ttcagcgatt ttcccttggt cgaggaagat aatccggtca gcaatggctt gggcaaactg
     2701 catttcgtgg gttactaaaa tcatggtacg gccttcttgg gccaaatcat tgataagttc
     2761 cagcacctca cgcaccattt ctggatccag cgaagcagtc acctcgtcaa aaaggatgat
     2821 ttctggatgc attaggaggg cacggacaat tgcaacacgt tgcttctgtc caccagataa
     2881 ttgacgggca aagctatgtt gtttatccag caaaccgaca cgatctagta attgcaaagc
     2941 ttcttccgtt acttctttct tgtcccttcc ttgagttttg atagggccta ggatgaggtt
     3001 ttgtaagaca tccagatggg gaaagagttc ataactttga aagaccatgc caatcttttg
     3061 gcgaactagg tgaaaatttt ttttattttc aacgatagac tgaccatcca gaaaaatatc
     3121 tccaccttga atactttcta agccgttgag gcaacgaagg agggtacttt tcccacaacc
     3181 agatggccct aggatgacaa caacttcccc ttttttgatt tctagagaaa gtccttggag
     3241 gatgggattg tctccgaagg atttttttag ttccttgatt tctaagatag tttcagacat
     3301 ttagtttctc caatgttttt ctaagtgagt ggatagtttg gaaatagggt agcaaactgc
     3361 gaaatataag actagaatgg ttccataaat ccaaaatgaa gcagttggga tggtcaggcg
     3421 attgctatcg atgatttgtt gtccaacttt ggtcacttcc acaaccccaa tcaaaacaac
     3481 taatgaagtg gttttaatca tccgagtgac aagattgata gcctgcggta gcagtcttct
     3541 caagacttgt gggatgatga tgtggtagta aagttgaaca ttagtcaagc cgagtgcctg
     3601 tccactttca aactgatgtt tagggagaga agtgatagct ccacgtacca agtctcccat
     3661 ttcagctgtt ccccagaggg taaaaacgat aatagctgaa gtctcacctg agatattgat
     3721 attaaagttt cgagccaagc caaagtaaac gatgaagagt agcaccagct ggggcatgat
     3781 acggataaat tccagataca atcgtgttaa aaatcgtatg attctagaat gggaggtcat
     3841 gatgattccc atgactgttc cgaacatcat ggataagagg acagacagga tggatatccc
     3901 aatcgtaacg cccaatccct gtaagattct caggagatta tttccttgaa agagtacttg
     3961 gattcccgaa tcctgcatgg cggagcctcc tttctatcca gctaaagacc agtgagatgg
     4021 gtagcagcat gatcagataa gcaactacca acatagctag cgcaatgtct gtctcatagt
     4081 agagatcaat caaatccttg gcgacgtaca tgaggtcggc caaagccact gctgagaaaa
     4141 cagaggtttc cttgataagg aaaatgacat tggcactaaa ggaggggagt gccaccgctg
     4201 ttgcttgcgg aagaaccaca tagtaaaaga cctgtagagg tgtcagacca atagcgaggc
     4261 caatctcctg ctgggtttga ctgatggctt ccagcccact tcggaaagat tctgccatat
     4321 aggagtctcc taaaaagaca agcccaagcg ttgcacagac ttctgaagat aggacaatcc
     4381 cgattcgggg aagaccgaag tagagaaaga agagttgaat caaaaggggc gtattacgtg
     4441 acaattcaat gtaggctgtc gctacttgcg acaaaacaag gattcgataa tgtcggatga
     4501 tactaacgat taaaccgagc agaaaggatc ccaaaattcc ccaaactgca atatgcaagg
     4561 tcagaaagaa tgccttttga tatagtggta gatattgttc aacaatggat caatccaaaa
     4621 atagaacctc ccatctagaa ataatacagt tattgtagca cttaaaatct tctttggata
     4681 atatctattt tttattgccg ttataaggat ttttatcata gacataaaat ttctgaaatt
     4741 tccaaacaaa atattttaaa agttttg
//
LOCUS       DAVLSL010000132         4751 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLSL010000132 DAVLSL010000000
VERSION     DAVLSL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.134"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            84..1259
                     /gene="mutY"
                     /locus_tag="VXN30_001756"
     CDS             84..1259
                     /gene="mutY"
                     /locus_tag="VXN30_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1358339.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1314..2018
                     /locus_tag="VXN30_001757"
     CDS             1314..2018
                     /locus_tag="VXN30_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1358340.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2011..3360
                     /gene="vicK"
                     /locus_tag="VXN30_001758"
     CDS             2011..3360
                     /gene="vicK"
                     /locus_tag="VXN30_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1358341.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3362..4171
                     /locus_tag="VXN30_001759"
     CDS             3362..4171
                     /locus_tag="VXN30_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1358342.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4262..4504
                     /locus_tag="VXN30_001760"
     CDS             4262..4504
                     /locus_tag="VXN30_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1358343.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMIHDLGLKDE"
     gene            4506..>4751
                     /locus_tag="VXN30_001761"
     CDS             4506..>4751
                     /locus_tag="VXN30_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1358344.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRD"
BASE COUNT         1455 a          831 c         1093 g         1372 t
ORIGIN      
        1 atagaaaagt tatatactat ctattgaaaa tattataaat cagatacaaa gacaggtgaa
       61 accttctgtg ttatactaga gatatgttag atttgaaaga atacggtatc gtcatgtggc
      121 cggaggagaa agtcatttct ttccgtgaga aacttctcgc ttggtatgat gaaaacaaaa
      181 gagatttgcc ttggaggaga agtaaaaatc cttatcacat ctgggtatct gaaatcatgc
      241 ttcagcagac cagggtggat acagttatcc cttactacga aagatttttg gactggtttc
      301 caactgtcga aagtctggca actgcgcctg aggagagttt actgaaagct tgggagggct
      361 tgggctatta ttctcgagtt cgcaatatgc aggctgcagc ccagcagatt atgactgact
      421 ttggtggcca atttccaaat acctatgaag gaatttccag cttgaaaggg attggacctt
      481 acacagcagg agccatttcc agtattgctt ttaacttgcc tgagccagct gtagatggta
      541 atgtcatgcg ggtcttggcg cgtctgtttg aagtcaacca cgatattggg attccaagta
      601 atcgaaaaat ttttcaggca atgatggaga tcttgattaa tccagatcgt ccgggtgatt
      661 ttaatcaagc cttgatggac ttagggtctg atattgagtc tcctgtaaat cccagacctg
      721 aagaaagccc agtcaaggac tttagtgcgg catatcagaa tggaaccatg gaccgttatc
      781 caattaaatc tcctaagaaa aagccggttc ctatttatct taaagcctta gttgtgaaaa
      841 attctcaagg acaattttta cttgaaaaaa atgaaagcga aaagctcttg gcaggttttt
      901 ggcatttccc ctttatagaa gttgataact tttcgcaaga agagcagttt gacctctttc
      961 atcaggttgc ggaagaaagt gtgaactctg gccccagtcc agaagagagt ttccagcagg
     1021 actatgacct agatgttgat tggcttgatg tttgttttga tacagtccag catgttttta
     1081 gtcatcgaaa atggcatgta cagattgtag caggtcaggt gagtgacttc catgattttt
     1141 cagataggga agttcgttgg ctttcaccag aagaattcaa gaattaccca cttgccaaac
     1201 cccaacaaaa aatctggcag gcttatgcac aagccaactt agacagtagc caagactagc
     1261 tattgtgtct tctgactatt ttttggtata atagtagaga aaaaggtgaa catatgaaaa
     1321 aaatactaat tgtagatgat gagaaaccaa tctcggatat tatcaagttt aatatgacca
     1381 aggaaggtta cgaagttgta actgctttta atggtcgtga agcgctagag caatttgaag
     1441 cagagcaacc agatattatt attctggatt tgatgcttcc agaaattgat ggtttagaag
     1501 ttgctaagac cattcgtaag acaagcagtg tgcccattct tatgctttca gccaaagata
     1561 gtgaatttga taaggttatc ggtttggaac ttggggcaga tgactatgta acgaaaccct
     1621 tctccaatcg tgagttgcag gcgcgtgtta aagctcttct gcgtcgttct caacctatgc
     1681 cagtagatgg gcaggaagca gatagtaaac ctcaacctat ccaaattggg gatttagaaa
     1741 ttgttccaga cgcctacgtg gctaaaaaat atggcgaaga actagactta acccatcgtg
     1801 aatttgagct tttgtatcat ttagcatcgc atacaggtca agtcatcacg cgcgaacact
     1861 tgcttgagac tgtctggggt tatgactatt ttggtgatgt ccgtacagtt gatgtgactg
     1921 tacgacgtct gcgtgagaag attgaagata cgcccagccg accagagtat atcttgacgc
     1981 gccgtggtgt agggtattac atgagaaata atgcttgatt tactgaaaca aaccattttt
     2041 accagagatt ttatctttat cctgattttg ttaggtttca tccttgttgt gaccctctta
     2101 ttactggaaa atagacgtga taatattcag ttgaagcaaa tcaatcaaaa ggttaaagat
     2161 ttgattgcag gagattattc caaggttctt gatatgcaag gtgggtctga aatcaccaat
     2221 attaccaata atttgaatga cttgtcggag gttattcgtc tcactcagga aaatctagaa
     2281 caagagagta agaggctaaa tagtattctg ttttatatga cagatggggt tcttgcgacc
     2341 aaccgtcggg gtcagattat catgattaac gatacagcca agaagcaact ggggttggtt
     2401 aaggaagatg ttctgaatag aagcattttg gaattgctca agatagaaga aaactatgaa
     2461 ttgcgtgatt tgattaccca aagtccagaa ttgttgctag attcccaaga tatcaatggc
     2521 gaatatttga accttcgagt tcgctttgcc ttgatacgtc gagagtctgg ctttatttca
     2581 ggtttggtgg ctgttttgca tgatacgacg gagcaggaga aggaagaacg cgaacgaaga
     2641 ctctttgttt ccaatgttag ccatgagtta cggactcctc tgactagcgt aaaatcctat
     2701 cttgaagcct tggatgaggg ggctttgtgt gaaactgtag caccagactt tatcaaggtt
     2761 tctcttgatg agaccaaccg tatgatgcgc atggtgacgg atctcctcca tctttcacgt
     2821 attgataatg ctaccagtca cctagatgtg gaactgatta acttcactgc ttttattacc
     2881 tttatcctca atcgttttga caagatgaaa ggacaggaaa aggagaaaaa atatgagttg
     2941 gtgagagatt atcccatcaa ttctatctgg atggaaattg atacagataa gatgacgcag
     3001 gttgtcgaca atattttaaa taatgctatt aagtattcgc cagatggggg taaaatcact
     3061 gtcagaatga agacaactga agaccagatg attttatcca tttctgacca cggtttgggg
     3121 attcctaagc aggatttacc acgtatcttt gaccgtttct atcgtgtgga tcgtgctaga
     3181 agtcgtgcac aaggtggtac aggtctagga ctgtctatcg ctaaagaaat tatcaaacaa
     3241 cataagggct ttatttgggc caagagtgaa tacggcaagg gttcaacctt taccattgtg
     3301 ctcccttatg ataaggatgc agtgaaagaa gaagtatggg aggatgaagt agaagactag
     3361 aatgagtgaa ataggcttta aatacagtat tttagcgtcg ggttccagtg gaaattcttt
     3421 ttatctggaa acctcaaaaa agaagctttt agtagatgca ggcttgtctg gcaagaaaat
     3481 taccagtctg ctagctgaaa ttaaccgtaa gccagaagac ctggatgcca tcttgattac
     3541 ccatgagcat tcagatcata tccatggagt aggcgttttg gctcgcaagt atggtatgga
     3601 tctttatgcc aatgaaaaga cctggcaagc tatggaaaat agtaaatatc ttggcaaggt
     3661 ggattcttcg caaaagcata tttttgaaat gggtaaaacc aaaacctttg gagatatcga
     3721 catcgagagt tttggtgtaa gccatgatgc agtcgcaccg cagttctatc gctttatgaa
     3781 ggatgataag agttttgtcc tcttgacaga tacaggttat gtcagtgacc gtatggcggg
     3841 cattgtcgaa aatgcggatg gttatcttat cgaggccaac catgatgtag agattttgcg
     3901 atcaggttct tacgcttggc gactcaaaca acgaatccta tctgaccttg gtcacctttc
     3961 taacgaggac ggtgctgaag ctatgattcg gacgctagga aatcgtacta agaagattta
     4021 ccttgggcat ttatctaagg aaaacaatat caaggaactg gctcatatga ccatggtcaa
     4081 tcagctggct caagctgatc tgggagtcgg agtagacttt aaggtttatg atacctcacc
     4141 agataccgca acaccattga cagagatata gaaagaacgc tgagaaggtg ttctttttat
     4201 attgactgga cacctaaaaa gtaatacaat ggtgttacca ttaaaaaagg gagcacaaaa
     4261 gatgactact attacattaa aagtttctga agctgataaa acatttatga aagcaatggc
     4321 taagtttgaa ggagtttccc tttcggaact tattcgcacc aaaactcttg aagccctaga
     4381 agatgaatac gatgctcgtg tggcagattt agcctatcaa gagtatttag aagacttgga
     4441 aaaaggagtt gaacccatta cttgggaaga aatgatccat gatttaggct tgaaggatga
     4501 ataatttgta taaattagtt ccaacaagac gttttatcaa gcaattgaaa aaattggacc
     4561 gttatacgca gaagctaatt acaaactatt tacaaaccaa tgttttggaa gacccaagac
     4621 gacacggaaa ggctttggtt ggtaatcgcg ttggtcaatg gcgctataga attggtaatt
     4681 atcgagttat cgtacaaatt gtagatgatg aattagtcgt tgctactcta gaagttggtc
     4741 atcggagaga t
//
LOCUS       DAVLSL010000133         4728 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLSL010000133 DAVLSL010000000
VERSION     DAVLSL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.135"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(14..304)
                     /locus_tag="VXN30_001762"
                     /pseudo
     CDS             complement(14..304)
                     /locus_tag="VXN30_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(350..583)
                     /locus_tag="VXN30_001763"
     CDS             complement(350..583)
                     /locus_tag="VXN30_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1358345.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(589..1581)
                     /gene="plsX"
                     /locus_tag="VXN30_001764"
     CDS             complement(589..1581)
                     /gene="plsX"
                     /locus_tag="VXN30_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1358346.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1578..2348)
                     /gene="recO"
                     /locus_tag="VXN30_001765"
     CDS             complement(1578..2348)
                     /gene="recO"
                     /locus_tag="VXN30_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1358347.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2345..3514)
                     /locus_tag="VXN30_001766"
     CDS             complement(2345..3514)
                     /locus_tag="VXN30_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1358348.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3663..4673
                     /locus_tag="VXN30_001767"
     CDS             3663..4673
                     /locus_tag="VXN30_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1358349.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
BASE COUNT         1242 a         1205 c          895 g         1386 t
ORIGIN      
        1 acttcctgca aaactagaat cctagttcat gattgataat accagcaatc aaattcattc
       61 gtaatccgaa gcgtttacga tgatttcgat aggttgttga aaacatttta aacgttttta
      121 ctttggcaaa gatattctca accttgcttc tctccttaga tagcgcatga ttacaggctt
      181 tatcttcaac tgttagcggc ttgagtttgc tggatttacg tggagtttgt gcttgaggat
      241 atatcttcat gagcccttga taaccactgt cagccaagat ttttccagct tgtccgatat
      301 ttctcatttc taaaaaccat ctacttccgt tgacttggaa ctccgattgc tattttcctt
      361 gaatgatttt aaccacatct cctacgtttt ggagttggtc aatttcttca tcgctgattt
      421 cgatactaaa ttcatcttcc agagtcaaga taaactccat caagtcaaca gaatccgcat
      481 ccaaatcgtc tttcagactc aaggattctg tcacgacaaa gtcctctccc tgtcgctctt
      541 ggataatggt cacaatacgg tcaaaaattt ctttttctgt catctctttt attctcctga
      601 aaattcacgc gcagtctggg caactacgtc tgtttctagc atggtacgaa tctggcgaat
      661 cgtactataa acagccttgg catcgcttga gccatgagtc ttgacaacag gtgccttaac
      721 accaaacaag accgctccac caacatctga ataattgagc tgttttttca aaccactgag
      781 gctgtccttg agaaggaggg cacctagttt cgctcgaaga ccaccacctg taatagctgt
      841 cttgagcaag cccatgattc ccatagctgt cccttcgatg gatttgagca cagcgtttcc
      901 cgtgaaacca tctgccacaa caacatctgc aacgccattc atcaaatcac gcgcttccac
      961 gtttccgata aagttcaaac tttcatcagc cgccagtaat tcataagttt ccttacgaag
     1021 cgggtcgccc ttgctactct ctgttccgtt gttgagcaaa ccaacgcgtg gttgcgcaat
     1081 gccacggaca tttttagcat agaaggaacc tagaaccgca tattgatgga ggtgctgggc
     1141 tgtattttct gcattggcac caaggtctag catgtcaaaa ccttttccat caacggtagg
     1201 caaggtagac atgagtccag gacggtcgat attcttgata cgacccacga tgaagaatcc
     1261 agcagccaac aaggcacctg tattcccagc cgaaaggaca gcgtctgctt caccatcttt
     1321 gacagccttg gctgccaata ccatactggc atttttctta ttcctaatag ctctcgtagg
     1381 ttcatcatcc gaatcaatct tctcatccgt atggataatg ctgacgcgct ctgtcgctgt
     1441 cagatattgc ttgattttag cttcatctcc gtaaagttga acctcgatat ctgaaaagtc
     1501 agatagggct tgattgacac cctcaacaat ggcctgaggt gcgtaatcgc cccccatggc
     1561 atctactgcg atttttttca tttcttttcc tcttttagta attgtcccca gtctgctagg
     1621 gaatcaataa atttctttga ttttaggtga atcccaacgt actcttcata taattgatcc
     1681 ataaattggc gtagctcttg cttgattcca ggcttgagcg aaatggtctc caaagtctca
     1741 aaatcaatag cttgaaattg attgagcaga taggggatat tgggattgag atgacaacgt
     1801 ctcttatcct catgataatg ctctggacag aggcaggctc catatttgaa agaaaagtca
     1861 aaagcctgac caacccgatg gcagaagaca cactcattaa aattgaggct gattccaaat
     1921 cgagtcaaaa tttgaatttc aaaaatattg gtcaaaacct gataatccaa gcctgcttcc
     1981 atcaactcca aagtcttttg caaaaaagca aacaagggag catcctgctg attgtcctgc
     2041 aaactagcat ctgcaagagc tgccacatag gtcgcatagg ccatgacaaa gaggtcacta
     2101 ttaatcttgg gaaaagtcat gacctcatga tagtcttcga tgtaactgag tccgtcatca
     2161 ttgattcgca atagaaatcg tgccagcacc aagggctgaa taacaggcgc cagcttagac
     2221 tgaccagcgt gtttgacaaa aaacatgcgt ttgccaacct gctctgtaaa aattttgacg
     2281 agcttgtcat cctcacgaaa attgcgattg taaagcacca agccttgact cgtgatagac
     2341 tgaatcatgc ttctctcatg tactcctcaa gtcgtttcat ggcttctttg atagtctcca
     2401 tgctggctgc ataagatagg cggacgtagc cttccccgta acgtccaaag gctgcaccag
     2461 ggataaaggc aacggccttc ttctgagcaa aatccttcag aaaagcaaag gagtcttgat
     2521 tgtagcccgc tggaatttta gcaaaaatat agaaggcacc gtctggtttg ataatctcaa
     2581 aaccaagagc agtcattttt tcgatgatat agtcccgacg ctggatatat tccttcttca
     2641 tgggctccgc atcattttta ccagccgtca aggcttctac cgcagcatgt tgcgccatgg
     2701 tatttgcggc agtgaccaag tattggtgac tcttgattaa ctgggctgtg aaggccgcag
     2761 gagcgaaaat cagccccaaa cgccaacctg tcatggcatg cgatttagac aaaccattga
     2821 taataatagc ctggtctctc aacatcgttc ctagagacac atgggcttcg cctgtgtagg
     2881 tcaattctga gtaaacctca tcacagacaa caaaaatttc gtacttgcgt aaaacagctg
     2941 ccaaggcctc taactgctct cgactgtagg taattcctgt cggattggct ggatagttga
     3001 gaataaccgc cttgagctta tcaccctgct ccaaaatggc cttctccaac atctcaggag
     3061 tcaagacaaa accattttca gtcgtatcaa tctcaacaat ttctgcccca actaagttaa
     3121 caatcggttc atagcctgga taagcaggag ctggcaaaag taccttgtcg ccctcttcca
     3181 aaatagccgt caaagtcgca gataaagcct ctgtcgcccc aattgtaacc aagatttcat
     3241 tttcaggagc atagtccagt tggtactttt ccttaacaaa gtcactggct gcctgacgta
     3301 gagtcagcag accactcatc cctgtatagt aggattggtt ctgatcaatc gctcgcttgg
     3361 ccgcctcctt gacatggtct ggcgttgtaa aatcaggttc ccccaaggtc aaacgcaaga
     3421 ccccaggaat ctccgaaata gcctggtcaa actgacgaat caacgaaact tgaattttat
     3481 ctaactgttt attaaagcgc ttagttaagt ccatagatac ctcctacttt caaaacgtat
     3541 ctctattata ctacaaaagc cccccgaccg caaaaataca ttagagaata gctttccact
     3601 ttctatttta cttttcctgt ttacaggtct ataatggtaa cagattctat ttggaggttt
     3661 ttatgtcatt tctatcaaaa aatggagcag gcatcttggt ctgccttctc atttccatcc
     3721 tatcttggta cttaggagga ttcttccctg tgattggcgc acccgttttt gcgattttta
     3781 tgggcatgct cctacatccc tttctctcgt cttataaaca actggatgct ggattgacct
     3841 ttagttccaa gaaattgctc cagtatgccg ttatcttact tggttttggt ctcaatatct
     3901 ctcaagtctt cgcagttggg caatcttcac tccctgtcat cctttccacc atttcaatag
     3961 ccttgattgt tgcctacctc ttccagcgct tctttgccct ggatacaaaa ctggctacct
     4021 tgattggagt aggttcttct atctgtgggg gttctgccat tgcagcgaca gcgcctgtta
     4081 tccatgctaa ggaaaaggaa gtagcccaag ccatctccgt tatctttttc ttcaatgtct
     4141 tggctgcgct catctttcca accctcggca cctggcttca tctatccaat gacggcttcg
     4201 ccctctttgc aggaactgcg gtcaatgata cttcctctgt aaccgccaca gctagcgctt
     4261 gggacagtct ctaccagacc aataccctcg aatctgcaac cattgtcaaa ctcacgcgca
     4321 ccctagctat catccccatc actctctttc tctcctactg gcaaagtcgc gagcaaaaaa
     4381 ataagcaagg cttacaactg aaaaaagtct tcccactttt tatcctttac ttcatccttg
     4441 catcgctcct aaccaccctt ctgacctctc tcggtgtgtc tagtagtttc tttacccctc
     4501 tcaaacaact ctctaaattc ctcattatca tggccatgag tgccatcggt ctcaaaacta
     4561 atctggtcgc tatggtcaaa tccagtggaa aatccattgt tctcggagcc gtctgctgga
     4621 ttgccatcat cctcaccagc cttggtatgc agacccttat cggcattttc taatactctt
     4681 cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgta tatatgtt
//
LOCUS       DAVLSL010000134         4654 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLSL010000134 DAVLSL010000000
VERSION     DAVLSL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.136"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            143..902
                     /locus_tag="VXN30_001768"
                     /pseudo
     CDS             143..902
                     /locus_tag="VXN30_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            942..1706
                     /locus_tag="VXN30_001769"
     CDS             942..1706
                     /locus_tag="VXN30_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1358350.1"
                     /translation="MISLFGLVAAKPVQANTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1719..2396
                     /locus_tag="VXN30_001770"
     CDS             1719..2396
                     /locus_tag="VXN30_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1358351.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIESFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPFYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPGFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2406..3065
                     /locus_tag="VXN30_001771"
     CDS             2406..3065
                     /locus_tag="VXN30_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1358352.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALVEI
                     IRGGLNAVDKGQYEVGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLVKRWQQAA
                     E"
     gene            3360..4550
                     /locus_tag="VXN30_001772"
     CDS             3360..4550
                     /locus_tag="VXN30_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1358353.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKIIHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         1319 a         1081 c          836 g         1418 t
ORIGIN      
        1 attgttagaa atcgatttga ttgtcccgat cgatttttcc tgttattata tcactatagt
       61 gaattttttc agatatgatt tgacaagaac attcttttat cgtagaatca tgttataaaa
      121 tctaacacaa gggagatctc tcatggcttt agtagaattt aaaaacgtcg aaaaatatta
      181 cggagactac cacgcactcc gcaacatcaa tctccgtttt gaaaaaagga caagttgttg
      241 tcctgcttgg accttctggc tctgggaagt ccactcttat ccgtacgatc aatggtttag
      301 aggctgttaa caaaggaagt ctcctagtca atgggcacca agttgctggt gccagccaga
      361 aagatttggt acctcttcgc aaggaagtcg gcatggtttt tcaacatttt aacctttatc
      421 cacacaaaac agtgttagaa aacgtaacac ttgcacccat tgaagttcta ggaattgata
      481 aaaaagaagc tgaaaaaacc gcccaaaaat atctggaatt tgtaaatatg tgggacaaga
      541 aagattccta tcccgccatg ctatctggtg gacaaaaaca gcggatcgcc atcgctcgtg
      601 gtcttgctat gcatccggaa ctcctcctct ttgatgaacc aacatctgct cttgatcctg
      661 agactatcgg agatgttcta gcagttatgc agaaactggc gcatgatggg atgaacatga
      721 tcatcgttac ccacgaaatg ggctttgctc gagaggttgc ggaccgcatt atctttatgg
      781 ccgacggaga agttttagta gatatgacag atgtcgatag cttttttgac aatccaagcg
      841 aacctcgtgc ccaacaattc ctcagcaaaa ttatcaacca cgaaagtgac aaagtcaaat
      901 aaggaggcgc ctatgaaaaa aaattctttt tatcagcatt attgattagc cttttcggcc
      961 ttgttgctgc caaaccagtc caggctaata ctagtatcgc agacattcaa aaaagaggcg
     1021 aactggttgt cggcgtcaaa caagacgttc ccaattttgg ttacaaagat cccaagaccg
     1081 gtacttattc tggtatcgaa accgacttgg ccaagatggt agctgatgaa ctcaaggtca
     1141 agattcgcta tgtgccggtt acagcacaaa cccgcggccc ccttctagac aatgaacagg
     1201 tcgatatgga tatcgcgacc tttaccatca cggacgaacg caaaaaactc tacaacttta
     1261 ccagtcccta ctacacagac gcttctggat ttttggtcaa taaatctgcc aaaatcaaaa
     1321 agattgagga cctaaacggc aaaaccatcg gagtcgccca aggctccatc acccaacgcc
     1381 tgattactga actgggcaaa aagaaaggtc taacctttaa atttgtcgaa cttggttcct
     1441 acccagaatt gattacttcc cttcacgctc atcgtattga tgccttttcc gttgaccgct
     1501 ctattctgtc tggctacact agtaaacgca cagaactact agatgatagt ttcaagccat
     1561 ctgactacgg tattgttacc aagaaatcaa atacagagct caacgactat cttgataact
     1621 tggttactaa atggagcaag gatggtagtt tgcagaaact ttatgaccgt tacaagctca
     1681 aaccatctag ccatactgca gattaaggag gacaccccat gacagattta tcatcttgga
     1741 cagcctattt tcaggatttt ggacaatttt tcaatggttt cctctttact cttgccctag
     1801 cgattgaatc ctttatcctc gccatggtct taggaatctt ctttggtgcc atgtcaacca
     1861 gtaaacgtcc aattttacgc attttagctc gcatctttgt tgaattttac caaaatactc
     1921 ccctcttggt gcagtttgtt attgtttttt atggcctacc tcttatcagt gaccatatca
     1981 tcatgattcc attttattgg acagccgttc tctgcgtggg actctatcac ggcgcttata
     2041 tcgctgaggt tattcgttca gggattcagt ctattcctag tggtcagatg gaggccgcct
     2101 tgtcgcaagg ttttacctat atcagtgcca tgcgcttgat tatcttgcct caggccttcc
     2161 gcatcattct ccctccattg accaaccaaa ttgttaacct catcaagaac acctctactg
     2221 tagctatcat ctctggagta gacttgatgt ttgtgactaa gtcttggtcg gctctcaacg
     2281 gaaactatat cccaggcttt ttaggtgctg ctcttctcta ctttgcccta tgcttccctg
     2341 ttgcccagtt tggtcgcaag atggagcaag ccaacaaaaa agcctattca ctttaggagg
     2401 ttactatgga atctatttta gaagttttaa ccccagataa cctagtcttt atctttaaag
     2461 gatttggctt gaccctctat atttctctga ttgccatcat cctctctact atcatcggta
     2521 cggtgctagc tgtcatgaga aatggcaaaa atcctgtctt gcgaatcatc tccagtattt
     2581 atatcgagtt tgtgcgcaac gttcccaacc ttctctggat ttttactatc tttttggtgt
     2641 tcaaaatgaa atccacacca gcaggtatta cagcctttac tctctttaca tcagcagcct
     2701 tggttgagat tattcgaggc ggtctcaatg ccgtagacaa gggacaatac gaagtaggaa
     2761 tgtcacaagg cttcacctca gcccaaatcc tctactacat cattctccca caagccatcc
     2821 gcaaaatgct accagccatc atttctcagt ttgttaccgt gattaaggat accagtctcc
     2881 tctactctgt tatcgcccta caagaactct ttggagccag ccaaattctc atgggccgtt
     2941 atttcgaacc agagcaggtc ttcagtcttt acatcctgat tgccctcatc tacttcagct
     3001 ttaacctagc aatttctagc ctgtctcata tgctagtcaa acgttggcaa caagctgcag
     3061 aataatactc ttcgaaaatc tcttcaaacc acgtcagctt taccttgccg tatatatgtt
     3121 actgacttcg tcagttctat ccacaacctc aaaacggtgt tttgagcagc ctgcggctag
     3181 cttcctagtt tgctctttga ttttcattga gtataaacag ccactctcca gttcaattgg
     3241 agagtttttt gatgatagta gatatttata gacctcaatt tgacaccata cattttctat
     3301 gataaaatgt aacaaattta tttacaacaa atcgaaatct aaatgaaatg gaattttcta
     3361 tgaaatcagt caaaggacta ctctttatca tagctagttt tatcttgact cttttgactt
     3421 ggatgaacac ttctccccaa ttcatgattc caggactagc tttaacaagc ctatctctga
     3481 cttttatcct agccactcgt ctcccactac tagaaagctg gtttcacggt ttggagaagg
     3541 tctacaccgt ccacaaattc acagcctttc tctcaatcat cctactaatc tttcataact
     3601 ttagtatggg cggtttgtgg ggctctcgct tagctgctca gtttggcaat cttgccatct
     3661 atatctttgc cagcatcatc cttgtcgcct atttaggcaa atacatccaa tacgaagctt
     3721 ggcgatggat tcaccgcctg gtttacctag cctatatttt aggactcttt cacatctaca
     3781 tgataatggg caatcgtctc cttacattta atcttctaag ttttcttgtt ggtagctatg
     3841 cccttttagg cttactagct ggtttttata tcatttttct atatcaaaag atttccttcc
     3901 cctatctagg gaaaattatc catctcaaac gcttaaatca cgatactaga gaaattcaaa
     3961 tccatcttag cagacctttc aactatcaat caggacaatt tgcctttcta aagattttcc
     4021 aagaaggctt tgaaagtgct ccgcatccct tttctatctc aggaggtcat ggtcaaactc
     4081 tttactttac tgttaaaaat tcaggcgacc ataccaataa tatctatgat aatcttcaag
     4141 ccggcagcaa agtaacccta gacagagctt acggacacat gatcatagaa gaaggacgag
     4201 aaaatcaggt ttggattgct ggaggtattg ggatcacccc cttcatctct tacatccgtg
     4261 aacatcctat tttagataaa caggttcact tctactatag cttccgtgga gatgaaaatg
     4321 cagtctacct agatttactc cgtaactatg ctcagaaaaa tcctaatttt gaactccatc
     4381 taatcgacag tacgaaagac ggctatctta attttgaaca aaaagaagtg cccgaacatg
     4441 caaccgtcta tatgtgtggt cctatttcta tgatgaaggc acttgccaaa cagattaaga
     4501 aacaaaatcc aaaaacagag cttatttacg aaggattcaa gttcaaataa gtaatcatac
     4561 tctcctggga tttcccagga gaatttttta tagccaaatg aaataagacc ggaacgatgg
     4621 aattttggca ttcaaatcaa ttgataagaa tatt
//
LOCUS       DAVLSL010000135         4555 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLSL010000135 DAVLSL010000000
VERSION     DAVLSL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.137"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..638)
                     /locus_tag="VXN30_001773"
     CDS             complement(<1..638)
                     /locus_tag="VXN30_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1358354.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSK"
     gene            complement(926..1888)
                     /gene="nrdF"
                     /locus_tag="VXN30_001774"
     CDS             complement(926..1888)
                     /gene="nrdF"
                     /locus_tag="VXN30_001774"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1358355.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(2076..4235)
                     /gene="nrdE"
                     /locus_tag="VXN30_001775"
     CDS             complement(2076..4235)
                     /gene="nrdE"
                     /locus_tag="VXN30_001775"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1358356.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDEEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4316..4534)
                     /gene="nrdH"
                     /locus_tag="VXN30_001776"
     CDS             complement(4316..4534)
                     /gene="nrdH"
                     /locus_tag="VXN30_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1358357.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         1319 a          853 c          942 g         1441 t
ORIGIN      
        1 aacttactat gatctgcgag taaatatttt ttattagaat tatttaaagc gatgcgttga
       61 gcctctccct cttcctcgct aaaagtagct agagctccgt tttgaatacc attacagcta
      121 acgaaagctt tagaaaattg gagattagag agattttgta gggtcaatgt gccaacaaaa
      181 gcacctgtaa tatcgcgata atttccacct atcaaaatca aatctgttaa ttttcgttcg
      241 cttaaaatca gaaaaacagg tagactgttg gttacgacgc ggatattgtc aataggcaac
      301 tcacgcgcaa aaaactctaa tgttgttcct ggtccaatga aaatagtttc tctttcttct
      361 actagactgc ctgcaaaatg ggctatttct tgtttttctg ccgtttggag ggcttgtttt
      421 tcaatatttg atcgctcatt agtcaaaagg gagttggttc gaagtttttc agctccacca
      481 tgcacacgaa tcagcaaatc tttatcagct aattcctgta aatagcgcct tgcagtcata
      541 tctgaaacgg ctatttcgtc cataatctgt ttaactgtta tggttccttt actatttact
      601 atctctaaaa ttttggttaa tttttcttgt ttgagcatat tatcacctcg tttcctacta
      661 ctatcttacc ataaacaaac tcatcattca aatacaaaaa caacaaaatg aaacaaaaac
      721 aaaaatatcg aagtttgttt tcaaaacttt cgatattttt gttgggttat tattttgttg
      781 tttctagttt actttttgat ggttaagagt ggtggagaat tatactcaat gaaaatcaaa
      841 gagcaaacta ggaagctagc cgcaggctgt acttgagtac ggcaaggcga agctgacgtg
      901 gtttgaattt gattttcgaa gagtattagt ccaaaccgta gttgtagtca tcatcttgca
      961 tggcttcaac ttcgccaaga aggtaaccat ttccgacttg agagaagaag tcatggttgg
     1021 aagttcctgt tgaaataccg ttcataacga ttgggttgac atcttcagct gaatctggga
     1081 aaagtggatc ttgtcccatg ttcatgagag ctttattggc attgtagcga aggaaggttt
     1141 taacctcttc agtccaacca acaccgtcat aaagactctc tgtgtagcct tcttcatttt
     1201 cataaagagt atagagtagg tcgtacatcc attctttgag tttttcttgc tcttcttcag
     1261 gtaattcatt gaaaccaagt tggaatttgt aaccaatgta ggttccgtga acagactcgt
     1321 cacgaataat caatttaatg atttctgcaa cgttggcaag tttgttgtta ccgagatagt
     1381 agaggggagt gaagaaacca gagtagaaga ggaaggtttc gaggaagacg ctggcaactt
     1441 tcttttcaag tgggctgccg tttaggtaga tttcgttgac aatctcagcc ttcttttgta
     1501 ggtaaggatt ggtattggtc cattcgaaaa tttcttcaat ctcagcctta gtattcaagg
     1561 tagaaaagat tgatgagtaa gatttagcgt ggacagattc cataaattgg atgttattga
     1621 aaacagcttc ctcatgtggt gtacggatgt ctgcgcgaag ggcttgaacc ccagtttcag
     1681 attgcatagt gtcaagaagg gttaaaccac caaaaacttt tccgaccaag tctttctctt
     1741 tgttagatag ctttctccag tcatccaagt cgtttgataa gggaatacgt gtatcgagcc
     1801 aaaattgctc cgtcagtttt tcccaagttg atttgtcgat gacatcttcg atggcattcc
     1861 agttaatggc tttgtagtaa gtttccattt aaaatctctt tctgtgttta gtattgcgaa
     1921 ctcacaatta tttctacttt accataattc tataggagta tcgcacaaaa agtcggaagc
     1981 ccgactttta aaatgttaca taaattatgt tatgacatag tagatttgat tttatcagtg
     2041 ctgcttaggg aaaaatagtg tttctatgct agaaactaaa tcacacagct ttcacattgg
     2101 ttggcgccga cttctccacc gtcatctgta aaggtacgga cgtagtagat agacttgatt
     2161 cccttgttaa aggcatagtt acgaaggatg gacaagtcac gtgtcgtttg tttattttcc
     2221 ctcttccatt cgtaaaggcc ttttggaatg tcactacgca tgaagagggt gagtgaaagt
     2281 ccttgatcca cgtgttcagt cgcagcagcg taaacatcga tgactttacg catatccata
     2341 tcgtaggcag aagtgtagta aggaatggtt tctgtagaca agccagcagc agggtagtag
     2401 attttaccaa ttttcttttc ctgacgttct tcaatacgtt gcgtaatcgg gtggatagaa
     2461 gcagaaacat cgttgatgta gctgatagaa ccatttggcg ctacagcaag gcgattttgg
     2521 tggtaaagac catcctcttg aaccttgtcg cgaagttcag tccaatcagc aacaccaggg
     2581 ataaaaacat ttttgaagag ttctttaaca cggtctgatg ttggaacaaa ttcaccagtt
     2641 acatacttgt caaagtaact tccgttagca tagtctgatt tttcaaagtt gtggaaggta
     2701 ataccacgtt cacgcgcaat attgtttgac tctaccaagg tccagtagtt cataagcata
     2761 aagtagatgc ttgtaaattc aacagactca ggtgaaccat attcaatgag ttgttgggca
     2821 aggtagctgt gcagtcccat ggcaccgaga ccaaaggtgt gggcttggct atttccatgg
     2881 tcaatcgttg gtacagctac gatatgtgaa ctatctgtaa cgaaagtaag ggcacgaacc
     2941 atagcacgga tagaacgacc aaaatcaggt gaagtcatca tgttaaccac gttggttgaa
     3001 cccaggttac atgaaacatc tgttcccatt tgaaggaatt cttgagcatc gttgatcaag
     3061 cttggttctt gaacttgaag aatctcagaa cacaagttac tcatgataat ctttccatcg
     3121 acaggatttg cacggttagc cgtatctatg ttgactacat aaggatagcc agactcttgt
     3181 tgcaatttag agatttcagt ttccaaatcc cgcgccttga tttttgtctt gcgaatattt
     3241 ggatttgcga ccaattcatc gtatttttca gtaatgtcga tgtaattgaa tggcacaccg
     3301 tattcttttt ctacagagta agggctgaag aggtacattt cttcattttt acgagccaat
     3361 tcgtagaatt tatcaggtac tacaacacca agtgatagag tcttgacacg tactttttca
     3421 tcagcgtttt ctttcttagt tgaaaggaaa gcgatgatat ctgggtgaaa gacgttgagg
     3481 tagacaacgc cggcaccttg acgttgcccc aattggttgg agtaagagaa gctgtcttca
     3541 aaaagcttca taacaggaac gacacctgaa gcagctcctt catagccttt gataggtgca
     3601 ccagcttcac gaaggttgct gagggtaatt cccacaccac caccaatacg tgaaagttga
     3661 agagctgagt tgatagaacg tccgatagag ttcatatcat ccgtcacttg gattaggaaa
     3721 caagatacca actccccacg acgagcacgt ccagcattca agaaggaagg agtagcaggt
     3781 tggtagcgtt ggtggatgat ttcattggca atatcgattg caacagcttc attcccatca
     3841 gcgaaataaa gggcgttgaa gaagacacgg tcttccatat tttcaagata gtattcaccg
     3901 tcattagtct ttaaggcata ttgattgtaa aatttataag ctgccatgaa tgacttgaat
     3961 tggaagtttt ggtctttgat aaattgagcc aattcttcca agaactctgg acggtatttc
     4021 ttgataaagg ctgtttcgat gtagttgtgt tcaatgaggt aattgatttt gtctttgatt
     4081 gaatcaaaaa ccatagtgtt tggaactaca ttttctttaa agaaagcatc caaggcttcc
     4141 tcatctttat gaagcatgat ttgtccatta acaggacggt taatttcgtt attaagacgg
     4201 aagtaagtca cgtcttcaag atgttttaat cccataaaat ttcccttatc taattacaaa
     4261 agaaaggctt ctaagttagc cctaaaagca gtttcttctg gatgatgtac taagattatg
     4321 ctaattgttt cagttttcct ggttggaaac ctgaaaagac ttcagttggt gtttggataa
     4381 caggagctgc gctaaaaccg agctctttaa cttgatcgac gtactcaggt tgctcatcaa
     4441 gattgatttc acgataagag acattattac tgtccaagaa acgcttggtc attttacatt
     4501 ggacacaatt gtttttagaa taaacggtta ccattgtgta actcctcttc aaaat
//
LOCUS       DAVLSL010000136         4525 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLSL010000136 DAVLSL010000000
VERSION     DAVLSL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.138"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            96..329
                     /locus_tag="VXN30_001777"
     CDS             96..329
                     /locus_tag="VXN30_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1358358.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            329..1663
                     /locus_tag="VXN30_001778"
     CDS             329..1663
                     /locus_tag="VXN30_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1358359.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1673..1927
                     /locus_tag="VXN30_001779"
     CDS             1673..1927
                     /locus_tag="VXN30_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1358360.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            2023..2418
                     /locus_tag="VXN30_001780"
     CDS             2023..2418
                     /locus_tag="VXN30_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1358361.1"
                     /translation="MSKKLNRKKQLRNGLRRSGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            2830..3279
                     /locus_tag="VXN30_001781"
     CDS             2830..3279
                     /locus_tag="VXN30_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV1358362.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            3266..3823
                     /locus_tag="VXN30_001782"
     CDS             3266..3823
                     /locus_tag="VXN30_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358363.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKVTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            3825..>4525
                     /locus_tag="VXN30_001783"
     CDS             3825..>4525
                     /locus_tag="VXN30_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1358364.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDINFTYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYF"
BASE COUNT         1393 a          809 c         1042 g         1281 t
ORIGIN      
        1 acaacctcaa aacactgttt tgagcaacct gcggctagct ttctagtttg ctctttgatt
       61 ttcattgagt attagaacat ataatggagg tcgtcatgga caatatcatc gatgtgtcaa
      121 ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta gtggagctgg
      181 gttttaaacc ccttgccaat cccttgatgc gcaatacagt tggtcgtaaa gtatcactta
      241 aacaaggttc taagctagca ggaactccta tggacaagat tgtacgcaca ctggaagcga
      301 atggctacga agtgattgga ttagactaat ggcagatgaa cggattcatg tcctacggga
      361 tattttatta gaattgcata atggcgcctc tcctgagtcg gttcaggagc gttttgatgc
      421 gacctttgcg ggtgtctcag ccattgagat ttcccttatg gagcacgagc tgatgaactc
      481 agactcaggt gtcacctttg aagatgttat ggaactctgt gatgtccatg ccaatctttt
      541 taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc acccagttcg
      601 tgtcttcaaa gaagaaaatc tggctctccg tgcggccttg attcgcattc gtagattgtt
      661 agatacctat gagtctatgg aagacgagga aatgctggcg gagatgcgta agggtttggt
      721 gcgtcagatg ggacttgtgg gtcaatttga catccattac caacgtaagg aagaactctt
      781 ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt ggggagtgga
      841 tgatcagatt agggaactct ttcaaacagc tctaacgata gccaagtcac taccagaagt
      901 gtcaattagc agtgtaaagg aagcttttga agcttttgcg acagagtttg aaagtatgat
      961 tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc aggatgactg
     1021 gcttcagatt gcggaggaga gcgatgccta tggctatgcc atcatccgtc cgtcagagaa
     1081 atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc ctgtacagct
     1141 agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc attttaccat
     1201 tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac aggcttttgg
     1261 taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcacctcc ctatggagat
     1321 tacctttgtc aataaagatg atattttcca gtattacaat gacaatacgc cagctgatga
     1381 gatgattttc aaacggacgc cgtctcaagt cgggcgcaat gtcgaactct gccatccgcc
     1441 taagtacttg gacaaggtca aaactatcat gaaggggctt cgtgagggga ccaaggacaa
     1501 gtatgaaatg tggttcaagt ctgagtcgcg aggtaagttt gtccacatca cctatgctgc
     1561 agtacacgat gaagacggag aattccaagg agtgttggag tatgttcagg atatccagcc
     1621 ctaccgtgag attgatacgg actattttcg tggattagaa taaggagaaa aaatgagtta
     1681 cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca tgattaacga
     1741 catggcgcac aaggaaagtc aaaaagttta cgaagaagac caggacgagc gtgccaaaga
     1801 tgccatgatt cgctacgaga gtcgcttgga tgcttatcag ttcttgcttg gtaagtttga
     1861 aaacttcaaa gcaggcaagg gattccatga tttgccagaa ggcttgtttg gtgagcgaaa
     1921 ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg tttatgttaa
     1981 atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa gaaactcaat
     2041 cgtaaaaaac aattacgaaa tggcctccgt cgctcaggtg ccttttcaag tactgtgact
     2101 aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc aagcgcagct
     2161 ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga gcaagctcaa
     2221 aaagtagcta attctgtaga agattttgca gcaaacttgg gtggacttcc acttgatcgt
     2281 gccaagactt tctatgatga aggaatcaag tctgcttcag atttcaaaaa ctggactgaa
     2341 aaagaactcc ttgccttgaa aggaatcggc ccagctacca tcaagaaatt gaaagaaaat
     2401 ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgagaaaaac ttgctacaat
     2461 agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg ctgagaagtc
     2521 cacaaatgtg tcaaaactgg ttgctaatga ctgaaaaaat gaaatatttc tgtcttttta
     2581 ttttaaagac taaagatgcg ggcttggccc gaaattgggt ggtaccgcgg ataaacacat
     2641 tcgtccctgt catgtagatg gcagggcttt tcttttgtgt cttagtcaaa ggaggttgtt
     2701 ataaaacaat catttaaaac taataaacta tattatggtt ttcctgtttt tattttagga
     2761 tatcaagatt agaattttgg ctataacatc acgacctgta gttcctctta tagtctagga
     2821 gattgggttg tgattggagt cgttgcgaga gagaatgccg cagagcagat caaacagtat
     2881 caaaaattta ctgtgaatat ttctgatgaa acttctatgc ttgcgatgga gcaggctggt
     2941 tttatcagtc atcaggagaa attggaacgt ttgggagtgc attatgaaat ttctgaacga
     3001 actcagattc ctattttaga cgcctgtcca cttgttttag attgtcgggt agataggatt
     3061 gttgaggaag acggtatttg ccacatcttt gccaagattc ttgagcgact tgttgcccca
     3121 gaactcctgg atgaaaaggg acattttaaa aatcaactgt ttgccccaac ctatttcatg
     3181 ggagatggat atcagcgcgt ttatcgctat ctggataagc gtgtagatat gaagggcagt
     3241 ttcatcaaaa aagcgaggaa gaaggatggc aagaactgag ctgccagata aaatcgaaac
     3301 agaacgtctc gttttacgag tccgtactgt ggcggatgct gaggatatct ttgactatgc
     3361 tagtttgcca gaggtcgcct atccagcagg ttttcctcca gtcaagacct tggaagatga
     3421 gatttattat ctggagcaca ttcttccgga gcgtaatcaa aaggaaaatc tcccagcagg
     3481 ctacgggatt gtcgtcaaag taacggataa gatcgttggc tctgtcgatt tcaaccatcg
     3541 ccatgaagat gatgtgctgg aaatcggcta taccttacac ccagactatt gggggcgagg
     3601 ttatgtgcca gaagctgcgc gtgccttgat tgacttagcc tttaaagatt tgggtcttca
     3661 caagattgaa ctaacttgct ttggatataa ccttcaaagt aaacgagtcg cggaaaagct
     3721 tggctttacc ctcgaagctc gcataagaga ccgaaaggat gttcaaggaa accgctgtga
     3781 cagtctgata tatggcttgc tgaagagcga gtgggaggaa taagatgagc gatgtaaaag
     3841 aggaagtaag ctctctatct gagaagcagt tacgccaaat tgatgttgag tatgctgaat
     3901 taaatgatag tgatattatc gagcgattag cttatttaga aattaacaac aatgaaaaac
     3961 gtattgtcat ctctgatatt gaaccaacca aggaaataat gtcagtctcg gaccaaattt
     4021 ttgagattca aaagaatttt caaaaaatca agaatatgtt cgaattgttt atttcagatg
     4081 tctcggattt tttaagtatt aaaaataaac ttgagtcaaa ggaattagaa attgaagagg
     4141 cagatgtaaa tcgtttcatg attcatttat tatccagtgg aaaattgttt gtcgatttta
     4201 acgaaaacca aattaaacaa aaatattcca aagatagtga ggagtttgat tgtatacatg
     4261 gttttgcaag ctatcagtat gatatcaatt ttacttatcg attttgccat tctttgagaa
     4321 attattcgca acatactgac ttaccaataa atgaggtaaa agctgttagc ccagatgatg
     4381 agactgttat aatagatttc tatattgatt tagattatct tttaaattcc aactttaaat
     4441 ggaaaaaatt gaaaggggag ttgataaaac taaaccaaga aacatctaag attgacgcta
     4501 tagctctagt gaaagaatac tttaa
//
LOCUS       DAVLSL010000137         4496 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLSL010000137 DAVLSL010000000
VERSION     DAVLSL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.139"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..299
                     /locus_tag="VXN30_001784"
     CDS             <1..299
                     /locus_tag="VXN30_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1358365.1"
                     /translation="TYEEMMTSDFFEVWFQKFFLPTLTTPSVIIMDNARFHRMGKLEL
                     LCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            complement(292..459)
                     /locus_tag="VXN30_001785"
     CDS             complement(292..459)
                     /locus_tag="VXN30_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1358366.1"
                     /translation="MKKRILSAGLTFTSALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(624..2231)
                     /locus_tag="VXN30_001786"
     CDS             complement(624..2231)
                     /locus_tag="VXN30_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1358367.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPMQEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2384..3868
                     /locus_tag="VXN30_001787"
     CDS             2384..3868
                     /locus_tag="VXN30_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1358368.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3963..4445
                     /locus_tag="VXN30_001788"
     CDS             3963..4445
                     /locus_tag="VXN30_001788"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1358369.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1305 a         1014 c          862 g         1315 t
ORIGIN      
        1 tgacttacga agagatgatg acgagcgact tttttgaagt atggtttcag aagtttttct
       61 taccaacatt aaccacacca tcggttatta ttatggataa tgcaagattc catagaatgg
      121 ggaagctaga acttttatgc gaagagtttg ggcataaact tttacctctt cctccctact
      181 cacctgagta caatcctatt gagaaaacat gggctcatat caaaaagcac ctcaaaaagg
      241 tattaccaag ttgcaatacc ttttatgagg ctcttttgtc ttgttcttgt ttcaattgac
      301 tatattatca gcgtaatagt ctaatagata gttaaatgta gttggatttc cactaaaggt
      361 tgatgagtaa gtttttgtat ctgaacctga ttggtcgcaa gcagctaaaa gcaaagcaga
      421 tgtaaaagtc aggcctgcac taaggatacg cttctttatg ttcgtcttct ttctccttaa
      481 tagtgggaat ttgtaaagtt aattgaattt cgagaatgaa ggtttataaa ccttggttat
      541 aaaaaacaaa ggatttctgc cttttataca gtcctccctt gtttttatac gatttcaatt
      601 ttaaattttt ctgcaaaaaa tatttatagt aattccacac agaaagcatc ccatggaact
      661 aagatttgtt tttcaaagac ttcttgagct agggtgtttt caatcaagac agatttgact
      721 tttccttcta ctgtcaagtc ttgctcttca ttggacaagt tagccacaac taggaagcga
      781 cggtcgccat ccttacgtat ataagcaaag accttatcag ccgtatcaag caattcaaag
      841 tcagctcgaa ttagccagct attctccttg cgaatttgga ccagtttctg ataggtatag
      901 aaaatagaat ctggatttgc cagcgcttct tggacgttga tcatctcgta atttggatta
      961 actgccaacc aaggttgacc tgttgagaaa ccagcgtttt tgctctcgtc ccattgcata
     1021 ggggtacggg cattgtcacg tccaataaca cggatactgt ccatgatttc ttgcatcgga
     1081 acaccttttt caagagcctc acgcgcatag ttgagagatt caatatcttc tacttgatct
     1141 agtgtttcaa acggatagtt ggtcatccca atctcctcac cttggtagat ataaggagtt
     1201 cctctcatga gatgaagtaa gattgcaaag gctttggcag atttttcgcg gtattcttgg
     1261 tcatttcccc agattgagac aatacgaggg aggtcatggt tgttccagaa gagggaattc
     1321 cagccgtcct caactcctaa ctctgtctgc catttgttga agatttcttt taacttagcg
     1381 atattcagct ctttttgata gtgccattta ggctgacctt cctgatactg aagaccgata
     1441 tgttcaaact ggaagaccat agacaattct tgcccctttg gatcagagta gagcttggca
     1501 atctctggcg ttgctcccca agtctcccct actgtcaaga gatccttatc tccaaaggtc
     1561 gcctgattca tttccttgag atagggatgg agcataggac cattattgac taccttctcg
     1621 tcaggaattt tgccaatcat gtcaataaca tccatacgga aaccaccaat acctttatca
     1681 atccagaagt tcatcatctc ataaattttc tggcgaagtt tttcattttc ccagttgaga
     1741 tccggctgtt ttttgctgaa aaagtggaga tagtattgac ctgacttttc atcgtattcc
     1801 caagcagacc cactaaagat agaatctagg tcattgggtt catcgcgcca gatatagtag
     1861 tctcgctcag ggctgtcagt attttcacag gcttcgacaa accaagcatg ttcatctgag
     1921 gtatgattaa ccaccaagtc catgatgata cgaatgtcac gcttcttagc ttccgcaatc
     1981 agttgatcca tatcctccat ggttccaaaa atcgttgcaa tcgcttgata atcagcaata
     2041 tcgtagccat tatcatccat agggctgtca taaacgggag aaagccaaat cgctgtgatt
     2101 cctagcttag ctagatagtc caacttactg gtaatacctg gcaaatcacc aactccatct
     2161 ccattactat ccataaaact ctttggatag acttgataga ctacggcatt atgccaccat
     2221 ttttcttgca tcttcttacc tcattatttt aataatctat agtatatgat agcacttttt
     2281 aaaattttgt gcaagcgttt gcttaatctt tttaatttct tttttagctc cgtagtttcc
     2341 taacttccaa ttttttatta taatagagtc cagaggtaaa aaaatgaaaa aacaagcttt
     2401 tagttctgaa caatatttga atttacaacg cgaccatatt ttggagcgca ttaaccaatt
     2461 tgacggcaag ctctacttag agtttggcgg taaaatgtta gaagatttcc acgctgctcg
     2521 tgtccttcct ggttatgaac ctgacaacaa aatcaagctc ttgcaagaat tgaaagagca
     2581 ggttgaggtt gtgattgcca ttaatgctag caacattgaa cattccaaag cacgtggcga
     2641 cttaggcatt tcttatgacc aagaagttct tcgtttgatt gacaaattca atgaattagg
     2701 gatttttgtt ggttccgttg tcattacaca atacgctggc caacccgctg cagatgcctt
     2761 ccgcaatcaa cttgagaaaa acggaattga ttcttatctt cattatccaa tcaaaggata
     2821 tccgacggat atggatcaca tcatttcccc agaaggcatg ggcaaaaacg actacatcaa
     2881 aaccagtcgc aacttgattg tcgtaaccgc tcctggaccc ggttctggaa aattggcaac
     2941 gtgtatgtcc aatatgtacc acgaccaaat caatggcatc aaatctggct acgctaaatt
     3001 tgaaaccttc cctgtttgga atcttcccct tcatcatcca gttaacttgg cttatgaagc
     3061 tgccacagct gaccttgatg atgtcaacat gattgacccc ttccatcttc aaacctatgg
     3121 agaaaccact gtcaactaca accgtgatat cgaaatcttc ccagtgctca agcgcatgtt
     3181 ggaacgtatt ctcggaaaat caccatacgc ttcaccgaca gatatgggtg tcaacatggt
     3241 tggtttcgct attacagatg acgaggctgc tgtcgaagct tctaaacaag aaatcatccg
     3301 ccgttactat caaacagttc ttgacttcaa agctgaaaaa gttggcgaag ctgccgtcaa
     3361 gaaaattgag ttgctcatga acgacctcgg tatcacacct gcagaccgta aggttgctgt
     3421 cgttgcgcgc caaaaagcag aagaaactgg tggaccagcc ctagcctttg aattgccaaa
     3481 tggggaaatc gtcactggta agaactcaga actctttggc cctacagccg ctgccttgat
     3541 caacgccatc aaaaaatcag ctgacatcgc taaagaagta aaattaatcg agccggaagt
     3601 tgttaagcca atccaaggtc ttaaaatcga tcatctcggt agccgcaatc cacgccttca
     3661 ttcaaatgaa atcctgattg cacttgctat cacagctaca gaaaatcctg atgctgcccg
     3721 cgctatggaa gaactcggca acctcaaagg aagcgaagcc cactcaacca tcatcttgac
     3781 tgatgaagac aagaatgtcc ttcgtaaact gggtatcaac gtaacctttg acccatacta
     3841 ccaatacgac cgcttatatc gtaagtaaag atttcaacct ctccagcctc ggagaggttt
     3901 tctctctgtc tcaggagcaa cctttatgat ataatgagag aagaaaactg aaaggattta
     3961 ccatgtcaaa agaagttatt gtcgaaagtt ttgaacttga ccacaccatt gtcaaagcac
     4021 cctatgttcg cttgattggg gaagaaacag gaccaaaagg agacatcatc tccaattatg
     4081 atattcgctt ggtgcaacca aacgaagact ctatccctac tgccggcctt cacactatcg
     4141 agcacctctt agccaaactc atccgtaccc gcattgacgg catgattgac tgttcaccat
     4201 ttggttgccg cacaggcttc cacatgatta tgtggggacg tcacaccagt gctaaaatcg
     4261 cagctgttat caaggattcg ctcaaggaaa tcgctgaaac tactacttgg gaagatgtcc
     4321 cagggacaac catcgaatct tgcggaaact acaaggacca cagcctcttc tctgctaaag
     4381 aatgggcaaa actcatcttg gaacaaggga tttcagatga tgcctttgaa cgtcatgtga
     4441 tttaaaatcg agtaggggat aatctctagc ccctctcaca ccaccgtacg tgccgt
//
LOCUS       DAVLSL010000138         4466 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLSL010000138 DAVLSL010000000
VERSION     DAVLSL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.140"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(308..859)
                     /locus_tag="VXN30_001789"
     CDS             complement(308..859)
                     /locus_tag="VXN30_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358370.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1209..2033)
                     /gene="nadE"
                     /locus_tag="VXN30_001790"
     CDS             complement(1209..2033)
                     /gene="nadE"
                     /locus_tag="VXN30_001790"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1358371.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2030..3490)
                     /locus_tag="VXN30_001791"
     CDS             complement(2030..3490)
                     /locus_tag="VXN30_001791"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1358372.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3606..4094)
                     /locus_tag="VXN30_001792"
     CDS             complement(3606..4094)
                     /locus_tag="VXN30_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358373.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4084..4293)
                     /locus_tag="VXN30_001793"
     CDS             complement(4084..4293)
                     /locus_tag="VXN30_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358374.1"
                     /translation="MVKNLKLKLARVELDLTQGQLADAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
BASE COUNT         1276 a         1039 c          829 g         1322 t
ORIGIN      
        1 tgaataaaat tggttgaatt taattcccta actcgataga ggattctatt ttaaatttaa
       61 caacagctag gattacacta taaaaagcac attaccacct gttcggctgg agaatctagt
      121 cgattagctc cctcttagtt acaagcttaa agcgtttttt aactaacact aaaccagatt
      181 aaagaaggaa ctagaacatc tgtcttgtac taatcaatat gctattttaa agtttcaaat
      241 aaacttggaa tgctaattat tatatttaca ataaatagtt gacttcgata gaaacagaaa
      301 atagaattca acattcttcc ctacttatac cataataaat aagatccgca agatagcctt
      361 ttctctctac accattaaca atggtttgta gataggacat tccagccttt tccatgacac
      421 gacctgaagc tgggttgaga ctggcataac gtgctctgac cttttgaaaa cctgcttgag
      481 taaaacaaaa gtccaagata gctttcaaag tctctgtcat cataccattt ccccagtaag
      541 ccttgcctaa cacatagcca atttcacagc ttaaatcagc ctcgtctatc ttaacaatgc
      601 tgatatctcc tatcacttgc tctgggtttt cttttagaca aatggcccat ttataatagt
      661 tgagattagt ataggaagca acccaattgc aaatcgagtt tcgagtgatt tcgacatcag
      721 gatggggatc ccaggtaaca taggtcagat tctcagcgga tgaagcccaa ttttgaaaca
      781 tggcttctgc atcactctcc acaaatcttc ttaaaatcaa acgttctgtc tgtaatattt
      841 gcgtaccgat agatttcatg atgctacctc acttccttat ttctatgctc gtagtaaaaa
      901 ggctcaactc agtaacagct agaaaacgag tttgtcttct aatttcttga cgcagtcgct
      961 gcctggtttg aaatgcgctc tagcaatctt tttcaaacct agtcatcgtt gcaggggtaa
     1021 gacgatgaaa tcgaactctt cgagttaccg aatctgcctc attttcttat ttcaagatga
     1081 gaggctgaaa acctccagtg gaggtttttc ttgcttttta atttcagggc aagaagctaa
     1141 aaaggtcccc cggaccttct tcgctttctc atttctaggc tcagggtaaa aacctccaat
     1201 ggaggttttt actcccaaaa gtcatcaaat acagtgatgg gtaagtggcg tttgtgttgg
     1261 cctttgtgcc accagttttc aatggtcgct tgagcttctg ggctgattgt tttgccttct
     1321 aggtaatcgt caatctctgc gtaggtgact ccaagtgcga cttcgtcagc taggcctggt
     1381 ttatcttctt ctaggtctgc cgttgggatt ttttcataaa gggctggctc tgcgccaagt
     1441 ttctgcaaga gctgttttcc ttggcgttta ttgaggcggt aaagagggag aatatccgca
     1501 ccgccgtcac caaacttggt aaagaaacct gtgatatttt ctgcggcgtg gtctgttcca
     1561 atgaccgctc cgctatggga accagcaagg gcatactgag caatcatacg gcaacgtgcc
     1621 ttgatattcc ccttgttgaa gtctgaaaca ggactacctg tcgcttcaac tgcagctgtc
     1681 atggcatcag ctgattcctt gatattcaca accaagctga catctggctg gatgaaggct
     1741 agggcttttt gagcatctgc ttcatcagct tgcactccgt atggcaggcg gacagcgata
     1801 aatttgtagc tgtcgtctcc cgtttcagct cgcagttctt ccatagctaa ttgcgccaaa
     1861 cgtcctgcca aggttgagtc ttgtccccca gaaatcccta gtacaaaggt ttttaggaag
     1921 ggatgttttt tcagatatct ttttaagaaa tcaatagaac gacggatttc ttcctgggca
     1981 tcaatcactg gtttgacacc cagctcttgg ataatcgttt cttgcaaact cattcttctt
     2041 ctccttcacc aagggcttcc ttgcgcatct tatcaatcaa gtccatctta tcttgccata
     2101 cgtcacgcgc caaatccact ggatagtgct gcggattgag cacacgctta tactcatccc
     2161 acaacttgtc aaattcctta cgggcataat cctgaatgtc agtcaaacta ggcaagttgt
     2221 aaactaatat tccttctttg aagatatcca ccaagagagg aacagcatca aaattacgaa
     2281 ccgtcttctt gatgtatgta taggtcggat ggaacatctt gatttctgtc atgtcgctaa
     2341 tatccacacc atcataagtg atgtagtcac cttctgactt gcctttttca cgactggtaa
     2401 tgcgccacac ctgcttctta cctggcgtcg acactttttc cgcattatta gacagcttaa
     2461 tcgtattgcg catctggccg ttttcatctt cgattgcaac aatcttgtaa accgccccaa
     2521 gagccggctg gtcataggct gtaatcagct tggtacccac accccagaca tcaatcttgg
     2581 ccttttgcat cttgaggtta aggatggtat tttcatctag atcattagaa gcataaatct
     2641 tagcctctgt aaatccagcc tcgtccagtt gctgacggac tttcttagaa atgtaggcaa
     2701 tatccccaga gtcaatccgc acacccataa agttaatctg atcacccagc tcacgcgcca
     2761 cctgaatggc agctggtaca ccgatgcgaa gggtatcata ggtatccaca agaaagacac
     2821 aatttttgtg ggtcgcagcg taagccttga aagcctcata gtcattgcca taaacctgta
     2881 ccaaggcatg ggcatgggtt cccaaaacag gaatgtcaaa gagcttaccc gcacgcacgt
     2941 tgctggttcc attggcgcca ccaatcaccg ctgcgcgtgt tccccagatg gccgcatcca
     3001 tttcttgagc ccgacgtgtc ccaaactcca tcaagggttc atcttcgata accgaacgaa
     3061 tacgagctgc cttcgtcgcc accaaggtct ggtagttgac gatgttcaaa agagccgttt
     3121 cgaccaactg acattgggct agaggtcctt ccacctgcac aatcggttca ttagcaaaaa
     3181 ccaaatcccc ttcttgggca gaacgaacgg tcaactccaa cttgaaattg cgaaggtaat
     3241 ccaagaacgc cccatgataa ccaagcgact ccaaataggc tatatcacta tctgaaaaac
     3301 gcaagtcttc aagatagttc acaattcttt ccaaacctgc aaaaaccgca tagccgttct
     3361 taaaaggctg ttggcggaaa tacacctcaa agaccgcctt cttattgtga atcccttggt
     3421 caaagtaaac ctgcatcatg ttgatctggt acaagtccgt gtgcaatgtc aaactatcat
     3481 ctggatacat acttttccta cttccttagc tagaaaccca tgaaaatttt caagaacttt
     3541 catgtattcc aataaattag tactattata tcacatttta gctggattga gaaaagagta
     3601 acaagctatt ctccactctc caattcatcc atatcttgtt caaatttttt ctgagcccat
     3661 tcgccatagc tcttaagacc aagattgcca ataaagaccc acggaaggta aatgacataa
     3721 gtaatgaccc aagcagacag gtatttaaaa ttcaaaggat tgtgctgata aatttctatg
     3781 ttgaattgat aattctgcaa catcaaaaga gccgtaatag ccaaggttag gaaaaaacaa
     3841 cccaaaatcg taaaatgaaa acgactatag taggtcactc ccagataacg ggcgcgattg
     3901 aaaaagtaaa atgtccctat gatgataacg attagcagca tattagaatt aaaaaggctt
     3961 ggtgctaata ctgaaatgat ataagatagg agcgacaaag caatgcagat atagaaactt
     4021 tcagagcccg ctttattgaa cagttgttct tctctttcgt ctagtaattg ataataataa
     4081 aatctatttt tcatcttctt cctcccaaaa tagttggtct agggttttcc ctaaacatct
     4141 gcaaatggac tggcagagcg agagactggg attgtatttt cccgcttcta tcaaaccaat
     4201 agtctggcgc gtcaccccga cagcatctgc tagttgacct tgagttaaat caagctctac
     4261 ccgagctaat tttaatttta aatttttaac caccttcgtc ctccttataa ttttaatatt
     4321 catctactct taaaaaattc caaactaaca caagctgtca gctatttatt ataactccat
     4381 taatttgcac tccagtatta tcttcagccg aaaatcagta ttttaggttg tttgaaataa
     4441 acttcctagt ttactctttt attttt
//
LOCUS       DAVLSL010000139         4358 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLSL010000139 DAVLSL010000000
VERSION     DAVLSL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.141"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            74..1063
                     /gene="manX"
                     /locus_tag="VXN30_001794"
     CDS             74..1063
                     /gene="manX"
                     /locus_tag="VXN30_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1358375.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1091..1894
                     /locus_tag="VXN30_001795"
     CDS             1091..1894
                     /locus_tag="VXN30_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1358376.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1918..2829
                     /locus_tag="VXN30_001796"
     CDS             1918..2829
                     /locus_tag="VXN30_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1358377.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2885..4219)
                     /gene="pepC"
                     /locus_tag="VXN30_001797"
     CDS             complement(2885..4219)
                     /gene="pepC"
                     /locus_tag="VXN30_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1358378.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
BASE COUNT         1187 a          941 c          923 g         1307 t
ORIGIN      
        1 aaacggttta tacttaaaca gtcttaaagt tttcttaaat gaaaactaaa acaaaaaagg
       61 aggaatgaca ataatgagta tcggaatcat tattgcgagc cacggcgaat ttgctgcggg
      121 tattcatcag tcaggatcta tgatctttgg tgaacaagaa aaggttcaag ttgtgacctt
      181 tatgccaaat gaaggtcctg atgatctata cgctaagttt aataacgctg ttgctgcatt
      241 tgacgcagaa gatgaggttc tagttttggc tgacctttgg agtggttctc catttaacca
      301 agctagtcgc gtgatgggag aaaatcctga gcgtaagttt gccatcatca caggacttaa
      361 cttaccgatg ttgattcaag cctacacaga gcgcctcatg gacgctgctg caggtgtaga
      421 aaaagtcgct gctaatatca ttaaagaagc caaggatggc atcaaagctc ttccagaaga
      481 gctaaatcca gttgaagaag ttgcaagcgc tgcagctgct ccagttgccc aagctgctat
      541 cccagaagga actgttatcg gagacggtaa attgaaaatc aatcttgccc gtcttgacac
      601 acgtctactt cacggtcagg ttgcaactgc ttggactcca gattcaaaag caaatcgtat
      661 catcgttgct tcagataacg tggctaaaga cgaccttcgt aaagaattga ttaaacaagc
      721 agctccaggt aatgtcaagg ctaacgtggt tccaattcaa aaactgattg agatttcaaa
      781 agacccacgt tttggagaaa cacatgccct tatcttgttt gaaacacctc aagatgccct
      841 tcgtgccatc gaaggcggcg tgccaatcaa gactcttaac gttggttcta tggctcactc
      901 aacaggtaaa acattggtca ataccgtttt gtctatggac aaagaagatg ttgcgacatt
      961 tgaaaaaatg cgtgacttgg gtgttgaatt tgatgtccgt aaagtaccaa atgattctaa
     1021 aaaagatttg tttgacttga ttaacaaagc caatgtcaaa taagccatta tttatgaaag
     1081 gattttaaac atgtctatta tttctatggt tttagtagtc gttgtagcct tctttgcagg
     1141 tcttgaaggc atcctcgacc agttccaatt tcaccaacca cttgtagcct gtacccttat
     1201 tgggcttgta acaggtcact tggaagcagg gattatcctc ggtggatcgc ttcaaatgat
     1261 tgcccttggt tggtcaaata tcggtgctgc tatcgctcct gatgctgcac ttgcttctgt
     1321 cgctgctgcc attatcatgg ttcttggtgg tgactttacc aagactggta tcggtgttgc
     1381 ccaagcggtt gctatccctc ttgccgtagc tggacttttc ttgacaatga ttgttcgtac
     1441 aatttcagtt ggtttggttc atactgcaga tgctgccgct aaaaaaggtg acttcggcgc
     1501 tgtggagcgt gcgcatttca ttgcgctact cttccaagga cttcgtatcg cgcttcctgc
     1561 agctcttctc cttatggtac caactgaaac tgtacaaagt atccttagtg ccatgccaga
     1621 ctggctcaaa gatggtatgg ctatcggtgg tggtatggtc gttgccgttg gttacgccat
     1681 ggttatcaac atgatggcaa ctcgtgaagt atggccattc ttcgctcttg gtttcgttct
     1741 cgctgctgtg tcagatatta ctctaatcgg attcggtgct atcggtgttg ctatcgctct
     1801 tatctacctt cacctttcta aaactggtgg aaatggtggc ggaggagccg caacttctaa
     1861 cgacccaatc ggcgatatcc tagaagacta ctaagataag aaaggactga aaacatcatg
     1921 actgaaaaac ttcaattaac taaatcagat cgtaaaaaag tttggtggcg ttcaaccttc
     1981 ttacaagggt cttggaactt tgaacggatg caaaacttgg gctgggctta tacactcatt
     2041 ccagctatca aaaaactcta tactaaaaaa gaagatcaaa tcgctgctct tgagcgtcac
     2101 cttgagttct tcaacactca tccatacgta gctgctccag tcatgggggt tactcttgcg
     2161 cttgaagaag aacgtgctaa cggtgtggaa atcgatgacg ctgctatcca aggggtcaaa
     2221 atcggtatga tgggacctct tgctggtatc ggtgacccag tattctggtt tacagtacgc
     2281 ccaatccttg gatctctcgg tgcttcactt gcccttactg gcaatatctt gggtccactc
     2341 ctcttctttg ttgcatggaa cttgattcgt atgtcattct tgtggtatgt tcaagagatt
     2401 ggatacaagg ctggatcaga aatcactaaa gatatgtctg gtggtatcct tcaagatatc
     2461 actaaaggag cttctatcct tgggatgttc attcttgctg tccttgttca acgctgggta
     2521 aatattaaat ttgctttcga tgtttctaaa gttcaactag atgaaaaggc ttatatccat
     2581 tgggataaat tgccagaagg gtctaaaggt atccaagaag cattcgcaca agtaggacaa
     2641 ggattgtctc aaactcctga aaaagttact actttccaac aaaacttgga tatgttgatt
     2701 cctggattat caggactact ccttacttta ctttgcatgt acttacttaa gaaaaaagta
     2761 tctccaatca ctattatcct tgccctcttc gcagtgggta ttgtggcaca tgttcttcac
     2821 atcatgtaat caagcaacta aaaaggaacc aggttctaaa atctgattcc ttttttctat
     2881 gcttttattc agccaaggct cccattggat cccatggtgc aagtacaatt ggttctgctc
     2941 cataggcagc ttgttcttct gctgtcagca attccttacg aacaacgatt tggtatgtgt
     3001 attcgtccat ccaagcgtct gaggcaacaa agtaaccatc tgtaccgacc ttgtctcccc
     3061 atgagttttc aaccttccac ttggttgatt taccattttc gtccaagtca acacctgtca
     3121 agaccatggc gtgggtcatc aagctttcgc tatagtccaa acgtccagcc ttgtcttgag
     3181 taagtttaat gtccatgctt gattcaaagt cataaacatc tgtcgcaagg atgccagcct
     3241 tacggttact gagctggccg acatcagaac caaaccaaac agtctcacct gcttgcattt
     3301 gggcaatcgc caattctttc aagcgctcca ttggaacgtt gatgtaacga actgcacggc
     3361 taccaaccac attccccaac atctcaactg tgtaagattt tccgtaaggt ttatcagcag
     3421 ttggagcatt gataacagaa acgtagtctt ctaaaggaag attgacatat ttcttgtaaa
     3481 actcttgtgg tgtgattcct ttttcacttt tgtagttgtt atctttatcg cgataagcaa
     3541 agtcaaactt gcgtggtgga agtcctaatg acatagcaag aaagttaaag atttcttgca
     3601 agaggtcttc tttcttagct tgaacagtcg cttgatctgc accagaaaca agcaagtcac
     3661 gcaagatttg agcatcttga cgaagcaatt tattaaggat cgcatttagc tcacgactgc
     3721 tgctagatga aacagactca ggataaactg acttaggcac gacaccgtat ttttcaaaga
     3781 gggaaacgac catatcccat tgaccgccat cttgttgagg tgtttggagt aagaagctaa
     3841 ccttgcggct agtcaattct tggtctgaag tcgcaatgac ttgctccaag aaccagtttg
     3901 atttctcata cttatcccag aagaaagtgt gggcttgtga caactcaaag ttctccaatt
     3961 tgtattgcga gatgagtttg tggcggaagg tgttgagagc cgcaaacatc cagcaacgac
     4021 cagacgcttt ctggttagtg accttgtcct tggttaaatc caatgagaaa acaggtgtgt
     4081 tgtctacatg gctttggcga cgttccagag ctgcaaaaat tccgttgtgg ctggcagcat
     4141 tttcaatcgc ttggtatttt acatttgctt catagttggc aaatagttta tcagtaaatg
     4201 attcttgaat cgcgttcata gattcctcct tttattctac agtctattat aactcttttc
     4261 acaaaggaag caactaaaaa acataaaagg cgaggacatt gtccctcacc tttttcgaat
     4321 ttatactctt cgaaaatctc ttcaaaccac gtcaacgt
//
LOCUS       DAVLSL010000140         4279 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLSL010000140 DAVLSL010000000
VERSION     DAVLSL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.142"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..1072
                     /gene="dnaN"
                     /locus_tag="VXN30_001798"
     CDS             <1..1072
                     /gene="dnaN"
                     /locus_tag="VXN30_001798"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1358379.1"
                     /translation="SSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNEDAG
                     LLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYP
                     RIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDS
                     HRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISF
                     YTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGV
                     VSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRP
                     FTLVPADTDEDFMQLITPVRTN"
     gene            1137..1331
                     /locus_tag="VXN30_001799"
     CDS             1137..1331
                     /locus_tag="VXN30_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1358380.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1415..2530
                     /gene="ychF"
                     /locus_tag="VXN30_001800"
     CDS             1415..2530
                     /gene="ychF"
                     /locus_tag="VXN30_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1358381.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2602..3171
                     /locus_tag="VXN30_001801"
     CDS             2602..3171
                     /locus_tag="VXN30_001801"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1358382.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3172..>4279
                     /locus_tag="VXN30_001802"
     CDS             3172..>4279
                     /locus_tag="VXN30_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1358383.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIY"
BASE COUNT         1466 a          669 c          851 g         1293 t
ORIGIN      
        1 tagttctaaa aatgccattc ctattttatc aacagtaaaa attgacgtga ccaatgaagg
       61 tattacttta attggttcaa atggtcaaat ttcaattgaa aattttattt ctcaaaaaaa
      121 tgaagatgct ggtttgttaa ttacttcttt aggttcgatc cttcttgaag cttctttctt
      181 tatcaatgta gtatctagtt tacctgatgt gactcttgat tttaaagaaa ttgaacaaaa
      241 tcaaattgtt ttaaccagtg gcaaatcaga aattacccta aaaggaaaag atagcgaaca
      301 atatccacga atccaagaaa tttcagcaag cactccttta atacttgaaa caaaattact
      361 caagaaaatt attaatgaaa cagcctttgc tgcaagtaca caagagagtc gtccgatttt
      421 aacaggtgtc cacttcgtat tgagtcaaca caaagagtta aaaacagttg caacagactc
      481 tcatcgccta agccagaaaa aattgactct tgaaaaaaat agtgatgatt ttgatgtcgt
      541 aattcctagc cgttctctac gcgaattttc agcggtattt acagatgata tcgaaactgt
      601 agagattttc tttgccaata accaaatcct ctttagaagc gaaaatatta gcttctatac
      661 tcgtctccta gaaggaaact atcctgatac agatcgcttg attccaacag actttaacac
      721 tactattact tttaatgtgg taaacttacg ccagtcaatg gagcgtgccc gtcttttatc
      781 aagtgcgact caaaatggta ctgtgaaact tgaaattaag gatggggttg ttagcgccca
      841 tgttcactct ccagaagttg gtaaagtaaa cgaagaaatc gatactgatc aggttactgg
      901 tgaagatttg accattagtt tcaacccaac ttacttgatt gattctctta aagctttaaa
      961 tagcgaaaag gtgaccatta gctttatctc agctgttcgt ccatttactc ttgtgccagc
     1021 agatactgac gaagacttca tgcagctcat tacaccagtt cgtacaaatt aagtgaaaga
     1081 ggttgagcct ggctcgcctc ttttatgata taatcgaaaa agaaaaggag agtagtatgt
     1141 atcaagttgg aaattttgtt gagatgaaaa aatcacacgc ttgtacaatc aagtcgactg
     1201 gtaaaaaggc taatcgttgg gaaattacac gtgtaggagc agatatcaaa ataaaatgca
     1261 gtaattgtga gcatgttgtc atgatggggc gatatgattt tgagcgaaaa atgaataaaa
     1321 ttattgactg agaaccctta gttagagggt tagcgcttta tccctttttg tgttataata
     1381 ttagggattg aaatgaaaac ggagaatgag aaatatggct ttgacagcag gtatcgttgg
     1441 tttgccaaac gttggtaaat caacactatt taatgcaatt acaaaagcag gagcagaggc
     1501 agcaaactac ccatttgcga cgattgatcc aaatgttgga atggtggaag ttccagatga
     1561 acgcctacaa aaactaactg aaatgataac tcctaaaaaa acagttccca caacatttga
     1621 atttacggat attgcaggga ttgtaaaagg agcttcaaaa ggagaagggc tagggaataa
     1681 attcttggcc aatattcgtg aagtagatgc gattgttcac gtagttcgtg cttttgatga
     1741 tgaaaatgtg atgcgcgagc aaggacgtga agacgccttt gtagatccac ttgcagatat
     1801 tgatacaatt aatctggaat taattcttgc tgacttagaa tcagtgaaca aacgatatgc
     1861 gcgtgtagaa aagatggcac gtacgcaaaa agataaagaa tcagtagcag aattcaatgt
     1921 tcttcaaaag attaaaccag tcctagaaga cgggaaatca gctcgtacca ttgaatttac
     1981 agatgaggaa caaaaggttg tcaaaggtct tttccttttg acgactaaac cagttcttta
     2041 tgtagctaat gtggacgagg atgtggtttc agaacctgac tctatcgact atgtcaaaca
     2101 aattcgtgaa tttgcagcga cagaaaatgc tgaagtagtc gttatttctg cgcgtgctga
     2161 ggaagaaatt tctgaattgg atgatgaaga taaaaaagag tttcttgaag ccattggttt
     2221 gacagaatca ggtgtagata agttgacgcg tgcagcttac cacttgcttg gattgggaac
     2281 ttacttcaca gctggtgaaa aagaagttcg cgcttggact ttcaaacgtg gtatgaaggc
     2341 tcctcaagca gctggtatta tccactcaga ctttgaaaaa ggctttattc gtgcagtaac
     2401 catgtcatat gaagatctag tgaaatacgg atctgaaaag gccgtaaaag aagctggacg
     2461 cttgcgtgaa gaaggaaaag aatatatcgt tcaagatggc gatatcatgg aattccgctt
     2521 taatgtctaa aaattaataa atggtgtcaa ttaggttgga aaaaaaattc caaccctttt
     2581 ggcttttgaa aggaaaaata aatgaccaaa ttacttgtag gcttgggaaa tccaggggat
     2641 aaatattttg aaacaaaaca caatgttggt tttatgttga ttgatcaact agcgaagaaa
     2701 cagaatgtca cttttacaca cgataagata tttcaagctg acctagcatc ctttttccta
     2761 aatggagaaa aaatttatct ggttaaacca acgaccttta tgaatgaaag tggaaaagca
     2821 gttcatgctt tattaactta ctatggtttg gatattgacg atttacttat catttacgat
     2881 gatcttgaca tggaagttgg gaaaattcgt ttaagagcaa aaggctcagc aggtggtcat
     2941 aatggtatca agtctattat tcaacatata ggaactcagg tctttaaccg tgttaagatt
     3001 ggaattggaa gacctaaaaa tggtatgtca gttgttcatc atgttttgag taagtttgac
     3061 agggatgatt atatcggtat tttacagtct attgacaaag ttgacgattc tgtaaactac
     3121 tatttacaag agaaaaattt tgagaaaaca atgcagaggt ataacggata aatggtgacc
     3181 ttattagatt tattctcaga aaatgatcag attaaaaaat ggcatcaaaa tttaacagat
     3241 aagaaaagac aactaatact tggtttatca acatctacta aggctcttgc aattgcaagc
     3301 agtttagaaa aagaagatag gattgtgtta ttgacgtcaa cttatggaga agcagaagga
     3361 cttgttagtg atcttatatc tatcttgggt gaggaactcg tctatccatt tttggtagat
     3421 gatgctccta tggtggagtt tttgatgtct tcacaggaaa aaattatttc acgggttgaa
     3481 gccttgcgtt ttttgactga ttcatctaag aaagggattt tagtttgtaa tatcgtagca
     3541 agtcgattga ttttaccgtc tcccaatgca ttcaaagata gtattgtaaa aatctcagtt
     3601 ggtgaagaat atgatcaaca cgcgtttatc catcagttaa aggaaaatgg ctatcgaaaa
     3661 gttactcaag tacaaactca gggcgaattt agtcttcgag gagatatttt agatattttt
     3721 gaaatatccc agttagaacc ttgtcgaatt gagttttttg gtgatgaaat tgatggtatc
     3781 aggtcatttg aagtagaaac acaattatcg aaagaaaata agacagaact cactatcttt
     3841 ccagctagtg atatgctttt gagagaaaag gattatcaac gaggacagtc agctttagaa
     3901 aaacaaattt caaaaacttt atcacctatt ttgaaatcat acctagaaga aattctttca
     3961 agttttcacc aaaaacaaag tcatgcagac tctcggaagt ttttatcttt gtgctatgat
     4021 aagacatgga ctgtctttga ttatattgaa aaagataccc caatattctt tgatgattat
     4081 caaaaattga tgaatcagta tgaagtcttt gaaagagact tagcgcagta ctttacagaa
     4141 gaattacaga atagtaaagc attttctgat atgcagtatt tttctgatat tgaacaaatc
     4201 tataaaaaac aaagtccagt gacctttttc tctaatcttc aaaagggttt aggaaatctc
     4261 aaatttgaca aaatttatc
//
LOCUS       DAVLSL010000141         4228 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLSL010000141 DAVLSL010000000
VERSION     DAVLSL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.143"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            696..1688
                     /locus_tag="VXN30_001803"
     CDS             696..1688
                     /locus_tag="VXN30_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1358384.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1690..2508
                     /locus_tag="VXN30_001804"
     CDS             1690..2508
                     /locus_tag="VXN30_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1358385.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2510..3295
                     /locus_tag="VXN30_001805"
     CDS             2510..3295
                     /locus_tag="VXN30_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358386.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3393..3611
                     /locus_tag="VXN30_001806"
     CDS             3393..3611
                     /locus_tag="VXN30_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1358387.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3596..3757
                     /locus_tag="VXN30_001807"
     CDS             3596..3757
                     /locus_tag="VXN30_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1358388.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
     gene            complement(4069..>4228)
                     /locus_tag="VXN30_001808"
     CDS             complement(4069..>4228)
                     /locus_tag="VXN30_001808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358389.1"
                     /translation="DGTDVVSSKHCFEVVDRTDEVSNHTHGKATLTWFEEIFYEYKSK
                     TFVNRDTL"
BASE COUNT         1175 a          732 c          912 g         1409 t
ORIGIN      
        1 tcatcagaat gataaaatta atcaattaat atctgattac aaacagaata tgaaagcttt
       61 ttatatcact attgaaaaat ttatacgaga tgatgaaagc cttaagtgtt attttacaaa
      121 ggttatttca agtcgttcca aggtaacaag tctagatcag attgaagctg ataaaacgat
      181 acaaagaaaa tattcaagtg agctaaaaaa atttattgga ttttataatg agattatttg
      241 tgaggaaaat agtttcctac atgtacgaaa gaggtggtcg agttggttta ggtagtcggt
      301 gcgtgagttg ataattctca gggtatggac ttctttttca tgaatgaggt aaaagagcag
      361 gtattgttta gagacaatca ttctgagcat attttctgga tagagggagt atccgatttt
      421 atgatcaaag ttaataccgc cctctggtga gaagatgagt aggttggtaa tttaaactat
      481 taaacagaat ttttgattaa aagtattatt tcatgagaga aatcctaatt tcacaatcta
      541 taggcaaacg cttgcatttc gttttttatt ggactataat aggttggtat aaagccttct
      601 gtagtaataa aatgtagaag gtgtagaaag taaggattta gaatatttgt agttaaaaac
      661 acaatgttgc tattccttac gatagggaga tagatatggc aatgatagaa gtggaacatc
      721 ttcagaaaca ttttgtgaag attgttaagg aaccgggctt gaagggggct ttgcgctcct
      781 ttattcatcc tgaaaagcag acctttgaag cggtcaagga tttgaccttt gaggttccaa
      841 aagggcagat tttaggattt atcggggcaa atggtgctgg gaagtcgaca accattaaaa
      901 tgctgacagg aattttgaaa ccaacatctg gtttttgtcg gattaacggc aagattcccc
      961 aggacaatcg gcaagattat gtcaaagata ttggcgtagt ctttggacaa cgcacccagc
     1021 tatggtggga tttggctctg caagagacct acactgtctt aaaagagatt tatgatgtgc
     1081 caaactcgct ctttcataag cgtatggact ttttgaatga agtcttggat ttgaaggact
     1141 ttatcaagga tcctgtgcgg actctttcac tgggacaacg gatgcgagcg gatattgcgg
     1201 cctccttgct ccacaatccc aaggttcttt ttttagatga gccgaccatt ggtttggacg
     1261 tttcggttaa ggataatatt cgtcgggcaa ttactcagat caatcaagag gaagaaacta
     1321 ccattctttt gaccactcac gatttgagtg atattgagca actttgtgat cggattttca
     1381 tgattgacaa ggggcaaaag atttttgatg gaacggtgag ccaactcaag gagacctttg
     1441 gtaagatgaa gactctctct tttgaactgc taccaggtca aagtcatctc gtctctcact
     1501 atgaaggtct gtctgatatg accattgata gacaaggaaa cagcctcaac attgaatttg
     1561 atagttctcg ctaccagtca gctgacatta tcaagcaaac cctatctgat tttgaaatcc
     1621 gcgatttgaa gatggtggat acggatattg aggatattat ccgtcgcttc taccgaaagg
     1681 agctctagga tgatcaaatt gtggagacgt tataaaccct ttatcaatgc aggggttcag
     1741 gagttgatta cttaccgagt caactttatt ctctatcgga ttggcgatgt catgggggct
     1801 tttgtggcct tttatctctg gaaggctgtc tttgattctt cgcaagagtc tttgattcag
     1861 ggcttcagta tggcggatat caccctctac atcatcatga gttttgtgac caatcttctg
     1921 actagatccg attcgtcctt tatgattggg gaggaggtca aggatggctc cattatcatg
     1981 cgtttgttgc gaccagtgca ttttgcggcc tcctatcttt tcaccgagct tggttccaag
     2041 tggttgattt ttatcagcgt tggccttcca tttttaagtg tcattgtctt gatgaaaatc
     2101 atatcgggtc aaggtattgt agaggtgcta ggattaactg tcctttatct ttttagctta
     2161 acgctcgcct atctgattaa ctttttcttt aatatttgct ttggattttc agcctttgtg
     2221 tttaaaaatc tttggggttc caacctactt aagacttcca tagtggcttt tatgtcgggg
     2281 agtttgattc ccttggcatt ctttccaaag gttgtttcag atattctctc ctttttgcct
     2341 ttttcgtcct tgatttatac tccagttatg atcattgttg gaaaatacga tgccagtcag
     2401 attcttcagg cactcctttt gcagttcttc tggctcttag tgatggtggg attgtctcag
     2461 ttaatttgga aacgggtcca gtcctttatc accattcaag gaggttagta tgaaaaaata
     2521 tcaacgaatg catctgattt ttatcagaca atacatcaaa caaatcatgg aatataaggt
     2581 ggattttgtg gttggtgtct tgggagtctt tctggctcaa ggcttgaatc tcttgtttct
     2641 caatgtcatc tttcaacata ttccattcct agaaggctgg acctttcaag agatagcttt
     2701 catttatgga ttttccttga ttcccaaggg aatggaccat ctcttttttg acaatctctg
     2761 ggcactaggg caacgcctag tccgaaaagg agagtttgac aagtatctga ctcgtcccat
     2821 caatcctctc tttcacatcc tagttgaaac ctttcagatt gatgccttgg gtgaactctt
     2881 agtcggtggt attttattgg gaacaacagt gaccagcatt gtttggactc ttccaaaatt
     2941 cctgcttttc ctagtttgta ttccttttgc gaccttgatt tatacttctc ttaaaatcgc
     3001 aacagccagt atcgcctttt ggactaagca gtcaggcgcc atgatttaca tcttctatat
     3061 gttcaatgac tttgctaagt atccgatttc tatttacaat tctcttcttc gttggttgat
     3121 tagctttatc gtgcctttcg cctttacagc ctactatcca gctagctatt tcttacagga
     3181 aaaggatgtg ttctttaacg taggaggttt gatgttgatt tctctggttt tctttgttat
     3241 ttcccttaaa ctttgggata agggcttaga ttcctacgaa agtgcgggtt cgtaaaagct
     3301 aaagtaagac taaaatcaag aaaggaactt atgatgtttg taattgaaga agtcaaggat
     3361 gaaaatcaaa aaaggcagtt gtcgttgagg ttttgaagga tttgccagaa tggtttggaa
     3421 tcccagaaag cacacaagcc tatatagaag gaaccacgac actgcaagtt tggaccgcct
     3481 atcaggagag tgatttgact ggatttgtaa ggttatccta ttcgagtgaa gattgtgcag
     3541 agattgattg tctcggcgta aaaaagctta tcaaggtaga aaaattggga gccaattgct
     3601 tgctacttta gagagtgaag ctcgtaaaaa agttggttat ctgcaggtca aaacagtggc
     3661 agaaggttct aataaagatt atgatcgaac aaatgacttt tatcgaggtc ttggctttaa
     3721 aaagttagag attttttctc agctatggaa tccgtaaaat ccttgtcaga ttttgattaa
     3781 aaagcttgaa taatattact tgacatctat tctcagagtg ccatactgta agtgtaatcg
     3841 ccgatttagc tcagttggta gagcaaggca ctcgtaaagc ctaggttata ggtagataaa
     3901 cgactgagga gttgaaaaaa tagataggta gaagataacc gttaagcctt attcttagcg
     3961 gttatttata ttgtttaata gcgctgatat ttcatcaatt atgcctgttt tcgtgtttct
     4021 ggtagttgtt caagtttatt gctactattt ttgatggtat gaatgtgctt ataatgtatc
     4081 ccggttaacg aaagttttgg acttatactc atagaaaatc tcttcaaacc acgtcaacgt
     4141 cgccttgccg tgcgtatggt tactgacttc gtcagttcta tccacaacct caaaacagtg
     4201 ttttgagctg actacgtcag ttccatct
//
LOCUS       DAVLSL010000142         4212 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLSL010000142 DAVLSL010000000
VERSION     DAVLSL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.144"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(385..1158)
                     /locus_tag="VXN30_001809"
     CDS             complement(385..1158)
                     /locus_tag="VXN30_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1358390.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            1334..2737
                     /locus_tag="VXN30_001810"
     CDS             1334..2737
                     /locus_tag="VXN30_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1358391.1"
                     /translation="MEAVLAIDLGANSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLLSQGKINEDTELK
                     DIMTNIADTKYYYPQLS"
     gene            2766..4058
                     /locus_tag="VXN30_001811"
     CDS             2766..4058
                     /locus_tag="VXN30_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1358392.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKNVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSNMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
BASE COUNT         1413 a          601 c          859 g         1339 t
ORIGIN      
        1 tctttctaat gagttgtttt gtcgcttttc attatagatc ttatgggatt tttttctaca
       61 ataaaatagg ctccataata tctatagtgg atttacccac tacaaatatt atagagccgg
      121 tttgatacta tgcgttttat tgtgggaagg tttactccat tttctcctga aattgagttt
      181 ttgtccagcc tctgttttta gggttgctaa gaaaataatg tcatgtggtg aatatttgta
      241 aatcagtcag cagacagaac gatactcttc gaaaatctct tcacatcatg tcagcttcgt
      301 ctttccgtgt ccatggggga cttgacttca tcagttttca aatctgtact ttgagcaagc
      361 tgagactagc ttcctatttg attttcattg aatatcagaa acccattctc catcaaataa
      421 ttcgactgcg tctaataatt tttgatctgg cacggtgtca gaaataaagg ttgtgtattt
      481 ggatagggga ttaattttaa aaaatccagt cttgtaaaat ttagaactat caatcagtaa
      541 gatggtttca tgggctttgt caataatatt cttttttgaa atagcttggc tgagagaagc
      601 ttcataaaca tattggtcat caatacctct tgctgaacaa aatgctaaat cgatattaaa
      661 atgatctaat aaagaatttt ccttatcata gttgaccacg gaacaggatt gatgtttgac
      721 ctcgccagat gtgataaaga ttttggagct atctttaaca gtttcagata gggtttgtgc
      781 agtatgtaaa ccatttgtaa aaataatcaa attatcaagt tcagaaagat agggacagag
      841 ttcgtagaca gtcgtactgg aatccagata gatacacata ccagaccgaa taaagtcttt
      901 agcgagacta gcgattagtc ttttttgcct agtactttct ccttcccgta tttgatgaga
      961 aagttcaatt gtgttcatag aggacagggt cacgtatccg tgctttcttt tgataagacc
     1021 ttgattttct aagaaaatta aatcacgacg taaggtactt gtgctggaga aagtgatttc
     1081 tgccagctct tttacggcaa ttcttttttt ctttttgata atttcaatca attcaagtac
     1141 acgttcatct tttatcataa gctcctccta atttatcatt tcaactatat tatagcacaa
     1201 attggaggaa tttgaattat ttttatgaat attgggttaa catttgaaca ttattcaagt
     1261 aagcgttcac atattgaaaa aataaaacgt ggggattata ataaagttaa tcaaggacga
     1321 agagagaaga aaaatggaag cggttttagc aatagattta ggtgcgaatt ctggaagagc
     1381 aatcgttggt tacctttctg aaaataaact agtaatggaa gaaataaatc gcttttctaa
     1441 tctacctatt agagtaaaag ggcatttatc ttgggatatt gactttctac tagctaaaat
     1501 tcttgaaagt atccgcttgg ctaatactag ttacaagatt ttatctatcg gtattgacac
     1561 atggggagtt gattttggac tgattgataa tgaaggtaag ctgttatcac aacctgttca
     1621 ttatcgtgat gaaagaacaa agggagtgtt aaaggaaata tctgaaatga ctgaattaga
     1681 aaaactgtat tcagagacag gaaatcagat tatggagata aataccttgt ttcaactctt
     1741 taaggcacgt caagaatctc ctgactcttt ctataagacc aataagattc ttttaatgcc
     1801 agatttgttt aattatctct tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac
     1861 aactcaatta tttgatccta ggagtcaaaa ttggaatcag aatatcttaa aactatttga
     1921 attggattca tctttacttc ctgaaattgt ttcagaggga aatgttcttg gaaggataaa
     1981 agaggagtat ggtttaggcg atattcctgt tgtgaatgtt tgtagtcatg atacagcaag
     2041 cgcgattgtc tcagtaccta agacagaagg tagtttattt atttcatcag gtacttggtc
     2101 tttggttgga gtggaactta cttcaccgct tcttactacc gaatccttca gttatggatt
     2161 tacaaatgaa gtcggtaaag atggagtgat tacatttctg aagaattgta cagggttgtg
     2221 gatcatagag gaactaagac gttcatttga acgaagaggg aaagcctatt cttttgatga
     2281 tattaggaca atggtggaga aagaaaaaga aaatcttcct ctgattgata ctgaatcaac
     2341 tgaatttgca acagaatctg atatgcacaa gactttgaca gaatatctag cttatcatca
     2401 tgaaactaga gagtggacag atggacaact atttaagatt gtttatgaaa gcctagctga
     2461 aacgtatagg aaagcgatag agttactaga agaactaact cataaggttt ataagaggat
     2521 atatgtgatt ggaggaggtg ctagagccag ttactttaac caaatgattg ctgatagaac
     2581 tggtaaagag gttcttacag gtttgactga ggctacagct gtggggaata ttgttgtgca
     2641 gctattgagt caaggtaaaa taaacgagga tacagagtta aaggatatta tgacaaatat
     2701 tgctgataca aaatattatt atcctcagct atcataaaca aaaataaaaa aacggaggtt
     2761 ttgtcatgaa caaaaaaagt cttttgaaat gcgctgttat cgggttagta gctacctttg
     2821 gtttagcagc ttgtggaaca tctaaggatg caagcggtgg aagttcttct ggtaaagaag
     2881 tattagaatt ttatcatggg tatcatcata gtgaagatga atggcctgta gcgaagacta
     2941 tgcgagattt atatgataaa tttgcagaag aacataaaga tagtggtgta gaatttaaac
     3001 caactcctgt aaatggtgat ttgaaggata taatgaataa caaggttgct agtggtgaat
     3061 ttcctgatgt gattgaccta gcaggtaatg cagtatcatt agctgcgatt gaacaaaaac
     3121 ttgttttaga tttgaaacca tatatcgatt caaataagct tgaaaaaaat gttggtttga
     3181 actataagca aaaccaaaaa gatggaaaaa tttatacagt tcacgaacag ttatttacaa
     3241 tgggtttgtg gtacaacaag gacatatttg caaaagcggg tgctaagaca cctgatcagt
     3301 ggaatacttg ggatgatttc actcaggcaa tggcaagcat tagaaaacaa gatggagtgt
     3361 atgcatttgg tgctggagaa ccatctattc gcttgtttaa tacagtatta ggaaccacag
     3421 aaaatggacg taaattatta gataaaccat taacaaaaga aggaattgaa tctaaagaat
     3481 ttgcagatgc tttgaaaatg gttatgaaag aaattcaagc taatggttct aagaatgctg
     3541 gtggcgatgc caatgcttat tccaaagatt tccaagaagg taaatctgca gtattcttta
     3601 atggtgtgtg ggcttctggt gagatgtcta agaacccaag tcttgcacct ggaatctatc
     3661 ctgcaggtgt agcaattagt tcttctggag gtggtatcac tatctcaagt aatatgtctg
     3721 aagctaaaca aaaactagct cttgagtttt tgaaatatat gactagcgat gatgtacaaa
     3781 aagtaatttt tgaaaaagta ggagcaaatc cttctaatga aaatgtaaat gtaaaagaac
     3841 tttcagaaaa gagttcagaa gctactacta aaattttagg acaagcaatc actcaagtta
     3901 agaatgcaaa agcagttgtt ccaactgtaa gtgatgtttg gggtggcgat gtacacacag
     3961 ctattatcaa tgcattgaca gaaagtgctg ctgaaaatgt agatgttgat caaaaagtaa
     4021 aatctactca agatgtgttg aaatcattga ttggttaatt gttttaggga gtgaaaacga
     4081 agctcagttg gcactgccaa ctgagcttga ttttacttat atagttgaaa aaaggagttt
     4141 gtttatggca caaaaaatta tgagcctaca aaataggaag aatcaaaaaa gacgatttat
     4201 ctttttattt ct
//
LOCUS       DAVLSL010000143         4195 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLSL010000143 DAVLSL010000000
VERSION     DAVLSL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.145"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(77..2335)
                     /gene="glgP"
                     /locus_tag="VXN30_001812"
     CDS             complement(77..2335)
                     /gene="glgP"
                     /locus_tag="VXN30_001812"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1358393.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2361..3878)
                     /gene="malQ"
                     /locus_tag="VXN30_001813"
     CDS             complement(2361..3878)
                     /gene="malQ"
                     /locus_tag="VXN30_001813"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1358394.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
BASE COUNT         1190 a          869 c          873 g         1263 t
ORIGIN      
        1 agccgcaggt tgctcaaagc accgctttga ggttgcagat aaagctgacg tggtttgaag
       61 agattttcga agagtattag ttcaagtgcc agatgtcttc attatactga gcgattgtac
      121 ggtcagatga gaagaatcct gctttagaaa tgttaacgat gactttatcc aaccatgcgt
      181 cacggtcttc gtagtcagca agcatttgct ctttgacttt gatgtagtct tccaagtcaa
      241 gaagagtcat gaaccagtct ttgttgatca attcattgta aagacgttcc aagcgctctt
      301 tgtttccagc tgcaagaact gcatcactaa cgatgaagtc aaccaatggt ttgatagctt
      361 cacgagcgta gaattcgctt gatttgtaag ctgcttttgc gtaaaggtcg ataacagttt
      421 ctgaatcttc accgaagatg tagatgtttt cttctccaac caactcagcg atttccacgt
      481 tagcaccgtc catagtacca agtgtcaaag ctccgttcaa catgaatttc atgttaccag
      541 tacctgaagc ttctttagaa gcaagtgaaa tttgttctga gatatcacat gctgggataa
      601 ggaaacttgc tgcagtaacg ttgtagtttt caaccataac tacttgcaag tgtggagcta
      661 ctgctggatc gttagcaata acttctgaca tgcaaaggat taaatggata atgtcttgag
      721 cgattgtgta ggctggagct gctttaccac caaagaagat tgtgattgga cgagcaggga
      781 tgttaccagc tttgatgtca aggtatttgt ggatcacgta caaagcgttc atttgttggc
      841 gtttgtactc gtgaagacgt ttgatttgga tatcaaagat agaatttgaa ttgatttcca
      901 caccttggtg ttctttcaag tgacgagcca atttacgttt gttgtgagcc ttgatgcttt
      961 ccaatttttc tttgacagct gctttgtctt cataagacaa aagtttttca agctcatctg
     1021 cttcatggtg ccaaccatct ccaagaatct catctaagta gtgagacaat cttgggttag
     1081 catgcataag ccaacgacgg aaagtgatac cgtttgtttt gttgttgaac ttttctgggt
     1141 aaaggtcgta gaaggctttc aactcagaat ttttcaagat ttcagtatgg agtgctgcaa
     1201 ccccgttaac actgtatccg tagtggatat ccatgtgagc catgtgaaca cgtccgctct
     1261 catcgatgat ttgaacagct ggatctttgt actctgcctt cacacgacgg tccaattctt
     1321 cgatgattgg taccaagtga ggaaccactt cttgcaagaa ttcaagaggc catttttcaa
     1381 gcgcttcagc aaggattgtg tggttagtgt aggcagtcat gctacgaaca attgagattg
     1441 cttcgtcaag ctcgatacca cgtgcagtta aaagacgaat caattcagga atcaccattg
     1501 atgggtgagt atcgttgatt tggacaactg cgtagtcagc aaggtcatgc aagttgcttc
     1561 ctttttcgat tgcttcgtcg atgatcaatt gcgcaccgtt tgaaaccatg aagtattgtt
     1621 ggaagatacg gagcaattca ccttgacggt cactatcatc tgggtaaagg aagagagtca
     1681 agttgcgagc gatatctgtc ttgtcaaagt tgataccatc tttaataata gaagaatcaa
     1741 ctgaatccaa gtcaaacaaa cgcaagcggt ttttagtcgc tgtttcataa ccagtaacat
     1801 caatatcgta aagagttgat gtcaaagtaa agtctgcaaa tggtacttgg tagctacggc
     1861 ttgagcgaac caaccagttt tgctctgtca accatgcatt tggaattgtt tcttgttggt
     1921 tgtttttaag aacttgttgg aaaagaccaa agtggtagtt aagaccaaca ccgtcaccat
     1981 tcaaaccaag agtagcaatt gagtcgataa agcaggcagc caaacgtccc aaaccaccat
     2041 taccaagaga tggttccaat tcaacttctt caacttcgat caagtcttta cctgcagctg
     2101 caagttcttt tttaacatcg tcgtaaagac caaggttaat caagttgttt gacaagagtt
     2161 taccaatcaa gaactcagct gagatgtagt aaactttttt cttaccagtg ttgactggtt
     2221 tttggctgct tgcaagcttg ctgtagttaa gaagagcaag gtaaagctct tcattgctac
     2281 attctgcaat ggttttattg taacgatttt gtacaaattc ttgtagtgat aacatgttta
     2341 gttgtctcct gattattgtc ttatttcttt aaatctacca aattttcatt aattcggcga
     2401 taaattgttg tcaagtcaag caaaccttcc tcgacagctg gtgtcaattg atcttcagtc
     2461 atacgccaag accagtttcc accaagggta gatgggaagt tcatacgagc tgcctcatcc
     2521 aattctagta aatcttgcat agttgcaatt gccataaagc taactgatga aaatactgta
     2581 cgaagcatag cgtgtaccac tgtttcgtat tctttacggt tcgtgtaacg agccatgtac
     2641 tcacgagtcg catcatcaat ctcattacgg taccaaccaa gaaccgtatt gttatcgtgt
     2701 gttcctgtgt acataactga gttagcaggt gccaagtgtg ggctatcaat gctttcgtct
     2761 tctgggttga aggcaaattg aagaatcttc attcctggga agccagtacg ttcacgtaat
     2821 tcgatcactt catctgtcat gaagccaagg tcttctgcga tgatgtttag ctcaccaagt
     2881 tcttccttaa cggctgcaaa aagcttgtag cctggacctt tcacccactc accaggtgct
     2941 gctgtatcgg aaccagcagg gatttcccaa taagattcga agccacggaa gtggtcgata
     3001 cgaacgatat cgtagatttt gaagctttca cgcaagcgtt caatccacca tttgtagccg
     3061 tctttgtcca ttgcttccca gtcatagatt gggttacccc aaagctgacc agttacagaa
     3121 aactcatctg gtgggcatcc tgcgatacaa gtagccttac cattgacatc tgttttgaag
     3181 agatgtggat ttgcccacat atcacttgaa tcttccgcta cgtagattgg catgtcccca
     3241 acgatttcaa tgtggttgtc gttagcgtaa gctttcaatt tcaaccattg ttggaagaag
     3301 aagtattgag tcacacggtg gtaaaccaac ttgtctgcca attgctcacg atagctttca
     3361 agtgctgaag ctttacgagc acgagcatct gcatctggcc attcagtcca agcaagattg
     3421 tcaaaatgct ctttgatagc catatactca gcaaagagct caagccatga ttggttgtct
     3481 tgagcaaatt tctcaaaatc tttaacatct ccgacttcaa agaaacgttt caccgctttt
     3541 tctaaaagag gacgacgtgc atagtagatt ttagcatagt caacttcaga cgcatcgcta
     3601 ccaaagtcaa ctccttcaag gtcactcgct cccaacaaac cttgctccac caagatatct
     3661 aaatcgataa aatgagtgtt tcctgcgaag gctgagaaag attggtaagg agaatccccg
     3721 taactagttg ctcctaatgg aaggatttgc cagtaacgtt gttttgtacg gaccaagaaa
     3781 tcaacgaagt cgtaagcact ttgaccaaat gatccgattc cgtaagctcc tggaagagaa
     3841 gagatgtgca tcaacacacc actttgacgt tttttcataa tagcacctcg tgtgttaaaa
     3901 taatggaacg ttgcgtattt tgcagacgca aacgtttgcg ttacttataa gtatactcct
     3961 tttcaagcat aattgcaagc gtttttaaaa aacttttttg attattttta aggagactaa
     4021 ctcattttag caaagataac ttaaaactat ctaatcctaa agtttgactc tcacgagaca
     4081 gtctacaaag tacaaaacct ccttagggag cttgaattta tatgcaacaa agcacaaacg
     4141 tctatagaaa atagataggg attagaaaca gggaggtagc ccctcctgat ttccc
//
LOCUS       DAVLSL010000144         4188 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLSL010000144 DAVLSL010000000
VERSION     DAVLSL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.146"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..970
                     /locus_tag="VXN30_001814"
     rRNA            <1..970
                     /locus_tag="VXN30_001814"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1022..1094
                     /locus_tag="VXN30_001815"
     tRNA            1022..1094
                     /locus_tag="VXN30_001815"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1055..1057,aa:Ala,seq:tgc)
     gene            1219..4120
                     /locus_tag="VXN30_001816"
     rRNA            1219..4120
                     /locus_tag="VXN30_001816"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1169 a          855 c         1233 g          931 t
ORIGIN      
        1 aaagcgagcg caggcggtta gataagtctg aagttaaagg ctgtggctta accatagtag
       61 gctttggaaa ctgtttaact tgagtgcaag aggggagagt ggaattccat gtgtagcggt
      121 gaaatgcgta gatatatgga ggaacaccgg tggcgaaagc ggctctctgg cttgtaactg
      181 acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgctg
      241 taaacgatga gtgctaggtg ttagaccctt tccggggttt agtgccgtag ctaacgcatt
      301 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc
      361 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct
      421 tgacatccct ctgaccgctc tagagataga gctttccttc gggacagagg tgacaggtgg
      481 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa
      541 cccctattgt tagttgccat cattcagttg ggcactctag cgagactgcc ggtaataaac
      601 cggaggaagg tggggatgac gtcaaatcat catgcccctt atgacctggg ctacacacgt
      661 gctacaatgg ctggtacaac gagtcgcaag ccggtgacgg caagctaatc tcttaaagcc
      721 agtctcagtt cggattgtag gctgcaactc gcctacatga agtcggaatc gctagtaatc
      781 gcggatcagc acgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc
      841 acgagagttt gtaacacccg aagtcggtga ggtaaccgta aggagccagc cgcctaaggt
      901 gggatagatg attggggtga agtcgtaaca aggtagccgt atcggaaggt gcggctggat
      961 cacctccttt ctaaggataa ggaactgcgc attggtcttg tttagtcttg agaggtcttg
     1021 tggggcctta gctcagctgg gagagcgcct gctttgcacg caggaggtca gcggttcgat
     1081 cccgctaggc tccattggtg agagatcacc aagtaatgca cattgaaaat tgaatatcta
     1141 tatcaaatag taacaagaaa ataaaccgaa aacgctgtag tattaataag agtttatgac
     1201 tgaaaggtca gaaaataagg ttaagttaat aagggcgcac ggtggatgcc ttggcactag
     1261 gagccgacga aggacgtgac aaacgacgat atgccttggg tagctgtaag taagcgatga
     1321 tccagggatt tccgaatggg ggaacccaac aggtaatacc tgttacccac atctgttaag
     1381 gatgtgagga ggaagacgca gtgaactgaa acatctaagt agctgcagga agagaaagca
     1441 aaagcgattg ccttagtagc ggcgagcgaa acggcagaag ggcaaaccga agagtttact
     1501 cttcggggtt gtaggactgc aatgtggact caaagattat agaagaatga tttgggaaga
     1561 tcagccaaag agagtaatag cctcgtattt aaaatagtct ttgtacttag cagtatcctg
     1621 agtacggcgg gacacgtgaa atcccgtcgg aatctgggag gaccatctcc caaccctaaa
     1681 tactccctag tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccggg
     1741 aggggagtga aatagaacct gaaaccgtgt gcctacaaca agttcgagcc cgttaatggg
     1801 tgagagcgtg ccttttgtag aatgaaccgg cgagttacgt tatgatgcga ggttaagttg
     1861 aagagacgga gccgtaggga aaccgagtct gaatagggcg ccttagtatc atgacgtaga
     1921 cccgaaacca tgtgacctac ccatgagcag gttgaaggtg cggtaagacg cactggagga
     1981 ccgaaccagg gcacgttgaa aagtgcttgg atgacttgtg ggtagcggag aaattccaaa
     2041 cgaacttgga gatagctggt tctctccgaa atagctttag ggctagcgtc gacattagag
     2101 attcttggag gtagagcact gtttgggtga ggggtccatc ccggattacc aatctcagat
     2161 aaactccgaa tgccaatgaa ttatggtcgg cagtcagact gcgagtgcta agatccgtag
     2221 tcgaaaggga aacagcccag accaccagct aaggtcccaa aataattgtt aagtggaaaa
     2281 ggatgtgggg ttgcacagac aactaggatg ttagcttaga agcagctatt cattcaaaga
     2341 gtgcgtaata gctcactagt cgagtgaccc tgcgccgaaa atgtaccggg gctaaaacaa
     2401 tttaccgaag ctgtggatac ctttataggt atggtaggag agcgttctat gtgtgatgaa
     2461 ggtataccgt gaggagtgct ggaacgcata gaagtgagaa tgccggtatg agtagcgaaa
     2521 gacaggtgag aatcctgtcc accgtaagac taaggtttcc aggggaaggc tcgtccgccc
     2581 tgggttagtc gggacctaag gagagaccga aaggtgtatc cgatggacaa caggttgata
     2641 ttcctgtact agagtatgta gtgatggagg gacgcagtag gctaactaaa gcagacgatt
     2701 ggaagagtct gtctaagcag tgaggtgtga attgagtcaa atgcttaatt ctataacatt
     2761 gagctgtgat ggggagcgaa gtttagtagc gaagttagtg acgtcacact gccaagaaaa
     2821 gcttctagcg tttaaacata ctctacccgt accgcaaacc gacacaggta gtcgaggcga
     2881 gtagcctcag gtgagcgaga gaactctcgt taaggaactc ggcaaaatga ccccgtaact
     2941 tcgggagaag gggtgctgac ttaaagtcag ccgcagtgaa taggcccaag caactgttta
     3001 tcaaaaacac agctctctgc taaatcgtaa gatgatgtat agggggtgac gcctgcccgg
     3061 tgctggaagg ttaagaggag tgcttagcgt aagcgaaggt atgaattgaa gccccagtaa
     3121 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
     3181 cccgcacgaa aggcgtaatg atttgggcac tgtctcaacg agagactcgg tgaaatttta
     3241 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
     3301 cagtttgata ttgagtgtct gtaccacatg tacaggatag gtaggagtct aagagatcgg
     3361 gacgccagtt tcgaaggaga cgctgttggg atactaccct tgtgttatgg ccactctaac
     3421 ccagataggt gatccctatc ggagacagtg tctgacgggc agtttgactg gggcggtcgc
     3481 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgca
     3541 gagtgtaaag gtataaggga gcttgactgc gagagctaca actcgagcag ggacgaaagt
     3601 cgggcttagt gatccggtgg ttccgtatgg aagggccatc gctcaacgga taaaagctac
     3661 cctggggata acaggcttat ctcccccaag agttcacatc gacggggagg tttggcacct
     3721 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     3781 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     3841 gcgggcgtag gaaatttgag aggatctgct cctagtacga gaggaccaga gtggacttac
     3901 cgctggtgta ccagttgtct tgccaaaggc atcgctgggt agctatgtag ggaagggata
     3961 aacgctgaaa gcatctaagt gtgaaaccca cctcaagatg agatttccca tgattatata
     4021 tcagtaagag ccctgagaga tgatcaggta gataggttag aagtggaagt gtggcgacac
     4081 atgtagcgga ctaatactaa tagctcgagg acttatccaa agtaactgag aatatgaaag
     4141 cgaacggttt tcttaagttg aatagatatt caattttgag taggtatt
//
LOCUS       DAVLSL010000145         4039 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLSL010000145 DAVLSL010000000
VERSION     DAVLSL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.147"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            277..1722
                     /gene="cps4A"
                     /locus_tag="VXN30_001817"
     CDS             277..1722
                     /gene="cps4A"
                     /locus_tag="VXN30_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1358395.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSLSVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKLSQNTDLT
                     VDQSSSYLVAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALSFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMIDLVNAQLESGGTYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1724..2455
                     /locus_tag="VXN30_001818"
     CDS             1724..2455
                     /locus_tag="VXN30_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV1358396.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSCHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            2464..3156
                     /gene="cpsC"
                     /locus_tag="VXN30_001819"
     CDS             2464..3156
                     /gene="cpsC"
                     /locus_tag="VXN30_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1358397.1"
                     /translation="MQDQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGTYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVIVPIDTRIVSVSVNDRVPEEASRIANSLREVAAKKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGGLGTSVIVLLLELLDTRVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            3166..3849
                     /gene="cpsD"
                     /locus_tag="VXN30_001820"
     CDS             3166..3849
                     /gene="cpsD"
                     /locus_tag="VXN30_001820"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEV1358398.1"
                     /translation="MPTLEIAQKKLEFIKKAEEHYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDAQKAKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GSYGNYGKK"
BASE COUNT         1296 a          646 c          838 g         1259 t
ORIGIN      
        1 ttaagaaata aggctacttt tctgggtctt gttcataatt agattgaaac tagagtagta
       61 gacctctgct tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttgtcatg
      121 ttcttatttc attttactat atttttgttt cgcgggaagt ctactaagat acttaaagat
      181 gcagatagta aaaaaagatg cagatagtaa aaaaatgtag acattaccgt aaaaaagtga
      241 tataatcata agatgttcaa tgtataggtg ttaatcatga gtagacgttt taaaaaatca
      301 ggttcacaga aagtgaagcg aagtgttaat atagttttgc tgactattta tttattgtta
      361 gtttgttttt tattgttctt aatctttaag tacaatatcc ttgcttttag atatcttaat
      421 ctagtggtaa ctgcgttagt cttactagta gccttggtag ggctactctt gattatctat
      481 aaaaaagctg aaaaatttac tatttttctg ttggtgttct ctatccttgt cagctcagtg
      541 tcgctctttg cagtacagca gtttgttgga ctgaccaatc gtttaaatgc gacttctaat
      601 tactcagaat attcgctcag tgtcgctgtt ttagcagata gtgagatcga aaatgttacg
      661 caactgacga gtgtgacagc accgactggg actgataatg aaaatattca aaaactacta
      721 gctgatatca agttaagtca gaataccgat ttgacagtcg accagagttc gtcttacttg
      781 gttgcttaca agagtttgat tgcaggggag actaaggcca ttgtcctaaa tagtgtcttt
      841 gaaaatatca tcgagtcaga gtatccagac tacgcatcga agataaagaa gatttatacc
      901 aagggattta ctaaaaaagt agaagctcct aagacgtcta agaatcagtc tttcaatatc
      961 tatgttagtg gaattgacac ctatggtcct attagttcgg tgtcgcgatc agatgtcaat
     1021 atcctgatga ctgtcaatcg tgataccaag aaaatcctct tgaccacaac gccacgtgat
     1081 gcctatgtac caatcgcaga tggtggaaat aatcaaaaag ataaattaac ccatgcgggc
     1141 atttatggag ttgattcgtc cattcacacc ttagaaaatc tctatggagt ggatatcaat
     1201 tactatgtgc gattgaactt cacttctttc ttgaaaatga ttgacttatt gggaggggta
     1261 gatgttcata atgatcaaga gttttcagct ctacatggga agttccattt cccagtaggg
     1321 aatgtccacc tagactctga gcaggctcta agttttgtac gtgaacgcta ctcactagcc
     1381 gatggagacc gtgaccgtgg tcgcaaccaa caaaaggtga ttgtggctat ccttcaaaaa
     1441 ttaacgtcaa ccgaagcact gaaaaattat agtacgatca ttgatagctt gcaagattct
     1501 atccaaacaa atatgccact tgagaccatg atagatttgg tcaatgctca gttagaaagt
     1561 ggtggaactt acaaagtgaa ttcgcaagac ttgaaaggta caggtcggat ggatcttcct
     1621 tcttatgcaa tgccagacag taacctctat gtgatggaaa tagatgatag tagtttagct
     1681 gtagttaaag cagctataca ggatgtgatg gagggtagat gaaatgatag acatccattc
     1741 gcatatcgtt tttgatgtag atgacggtcc caagtcaaga gaggaaagca aggctctctt
     1801 ggcagaatcc tacaggcagg gggtgcgaac cattgtctct acctctcacc gtcgcaaggg
     1861 catgtttgaa actccggaag agaagatagc agaaaacttt cttcaggttc gggaaattgc
     1921 aaaagaagta gcagatgatt tagtcattgc ttatggtgca gagatatact atactctgga
     1981 tgctctagaa aagctagaaa aaaaagaaat tcctaccctt aatgatagtc gttatgcctt
     2041 gattgagttt agcatgcata cttcctatcg tcagattcat acgggattga gcaatatttt
     2101 gatgttggga atcacaccag taattgctca tattgaacgt tatgatgctt tagagaataa
     2161 cgaaaaacgt gttcgtgaac tgattgatat ggggtgctat actcagataa atagttgtca
     2221 tgtttcaaaa cctaagttct ttggtgaaaa atataaattc atgaaaaaga gagctcggta
     2281 ttttttggaa cgtgatttag ttcatgtagt tgcaagtgac atgcacaatt tagacagtag
     2341 acctccatat atgcaacagg catatgatat cattgctaag aaatatggag cgaaaaaagc
     2401 gaaagaactt tttgtagata atcccagaaa aattataatg gatcaattaa tttaggagaa
     2461 aatatgcaag atcaaaacac tttggaaatc gatgtattgc agctattcag agctttatgg
     2521 aaaagaaagt tggtcatttt attagtggca attataactt cttcagttgc ttttgcctac
     2581 agtacttttg ttatcaaacc tgagtttact agtacgactc ggatttatgt agttaaccgt
     2641 aatcaggaag agaagtctgg tttaaccaat caagacttgc aggcaggaac ttatctggta
     2701 aaagactacc gtgaaattat cctatcgcag gatgttttgg aggaagttat ttctgatttg
     2761 aaactagatt tgacgccaaa aggtttggct aataaaatta aagtaatagt accaattgat
     2821 acccgtattg tctctgtttc agttaatgat cgagttcctg aagaagcaag ccgtatcgct
     2881 aactctttga gagaagtagc tgctaaaaaa attatcagta ttactcgtgt ttctgatgtg
     2941 acaacactgg aggaggcaag accggcgata tcaccgtctt cgccaaatat taaacgcaat
     3001 acactaattg gttttttggc aggggggctt ggaactagtg ttatagttct tcttcttgaa
     3061 cttttggaca ctcgtgtgaa acgtccagaa gatatcgaag atacactgca gatgacactt
     3121 ttgggagttg taccaaactt gagtaagttg aaataggaga gaggaatgcc gacattagaa
     3181 atagcacaaa aaaaactgga gttcattaag aaggcagaag aacattacaa tgccttgtgt
     3241 acaaatatac agttgagcgg agataaacta aaagtaattt ccgttacttc tgttaaccct
     3301 ggggaaggaa aaacaactac ttccgtaaat atagcaaggt cgtttgcgcg tgcaggctat
     3361 aaaactcttt tgatcgatgg cgatactcga aattcagtta tgtcaggatt ttttaaatct
     3421 cgtgaaaaaa ttacagggct aacagaattt ttatctggga cagctgattt atctcacggt
     3481 ttatgtgata caaatattga aaatttattt gtagttcaat cgggatctgt atcaccaaac
     3541 cctacagcct tgttacagag taaaaatttt aatgatatga ttgaaacatt gcgtaaatat
     3601 tttgattata tcattgttga tacagcacct attggaattg ttattgatgc ggcaattatc
     3661 actcaaaagt gtgatgcgtc catcttggta acagcaaccg gtgaggtgaa taaacgtgat
     3721 gcccaaaaag ctaaacaaca attagaacaa acagggaaac tgttcctagg agttgtttta
     3781 aataaattgg atatctcggt tgataagtat ggggtatacg gttcctatgg aaattatggt
     3841 aaaaaataac ttaggaaaga ttttatggat gaaaaaggat tgaaaatttt tctggcagta
     3901 ttacagagta ttattgtcat tttattggtt tattttctta gctttgttag agagacagaa
     3961 gttgaacgtt cttcgatggt tatactatac cttctccact tttttgtatt ctattttagt
     4021 tcctatggta acaattttt
//
LOCUS       DAVLSL010000146         4037 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLSL010000146 DAVLSL010000000
VERSION     DAVLSL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.148"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            20..973
                     /locus_tag="VXN30_001821"
     CDS             20..973
                     /locus_tag="VXN30_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1358399.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1050..3038
                     /gene="uvrB"
                     /locus_tag="VXN30_001822"
     CDS             1050..3038
                     /gene="uvrB"
                     /locus_tag="VXN30_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1358400.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3048..3611
                     /locus_tag="VXN30_001823"
     CDS             3048..3611
                     /locus_tag="VXN30_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358401.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3577..>4037
                     /locus_tag="VXN30_001824"
     CDS             3577..>4037
                     /locus_tag="VXN30_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358402.1"
                     /translation="MTVIIKSMETPEEIEDKSLVHWQTWREAYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIK"
BASE COUNT         1182 a          713 c          938 g         1204 t
ORIGIN      
        1 aagaaaagag gacggggata tgaagaacaa aagaatattt aaagacttcc aagcttcaaa
       61 aatgagttta aacatttaca caagcccctt gttagccttt gtttttgtct tcataggaga
      121 gtttgtggct tttactttgt atggtattgg cttgttagct ctcatcggac ttgctagaaa
      181 ttttggagag gctggtcaaa atcttgcaag ctacttgcag accttgcatc agagcttgac
      241 ggataaaaca agtgactttc gtttaatttt aggattactg gcctttggtt ttattcttaa
      301 cactgtgttc agatggacaa gaaaagttga gaaaagacct attcgaacct tgggatttta
      361 tagagagaat ttcctcagca atcttctgaa aggatttagt ctaggcctgg cactttttct
      421 tctgaccttg ttaggtttag tggtcttagg tcaatatcgt ttggaatcca ttcacttgaa
      481 tccttattct cttgcctttg tcgtctttac tatcccattt tggattttac aggggacagc
      541 agaagaagtg gtggcccgtg cttggctact tcctcaattg gcctcaagaa ccaatctaaa
      601 actagctatt cttatatcta gcctgttctt taccctgctt catatgggca attctggtct
      661 cacccctcta tctctagtaa atctcttttt attcggagtt gccatggctc tttaccttct
      721 caaaactgat acagtttggg gtgttgcagg tattcatggt gcttggaatt ttgctcaggg
      781 taatctcttt gggattttag ttagtggtca accgtcagga acgtctctga tgaccttttt
      841 accacaaggc aatcaagatt ggctatcagg tggttctttt ggcatagaag gttccattat
      901 gacaagtctg gtattactac tgctgattgt ctatcttgct aataaattaa agaaagaaaa
      961 tgaaaggatg tgacttcggt ccgtcctttt cttcgtgaaa atactataag tatgctaaaa
     1021 taggaatagc acatggagag aggattctta tgatcaatca cattacagat aatcaattta
     1081 aactagtatc aaaatatcaa ccatcaggag atcaacccca agctatcgag cagttggtgg
     1141 ataacattga ggggggagaa aaagctcaga ttctgatggg ggcgactgga acagggaaga
     1201 cctatactat gagtcaggtc atttctaaag tcaataaacc aactctggtt attgcccaca
     1261 ataaaactct ggctggtcag ctctatgggg agtttaagga atttttccct gaaaatgcag
     1321 ttgagtattt cgtatcctac tatgattatt accagccaga ggcctatgtc ccttctagcg
     1381 atacctatat tgagaaggat agttctgtca atgacgagat tgacaagctt cgccactcag
     1441 ctacctcagc ccttttggag cgtaatgatg ttattgtcgt ggcctcagtc tcttgtatct
     1501 atggtttggg ttcgcccaag gaatacgctg atagtgtcgt tagtctccgt cctggtctag
     1561 agatttctcg tgataaactc ttgaatgact tggtcgatat tcagtttgaa cgtaatgata
     1621 ttgatttcca acgcggaaga tttcgcgttc gtggggatgt ggtagagatt ttcccagctt
     1681 cccgagatga acatgccttt cgagtagaat tttttggaga cgaaattgac cgtattcgtg
     1741 aagttgaggc tctgacaggt caggtgttgg gagaagtgga tcatttagcg attttcccag
     1801 cgacacactt tgtgaccaat gacgaccaca tggaagttgc cattgcaaag attcaggccg
     1861 agttggaaga acaattagct gtctttgaaa aggaaggtaa actgcttgaa gcccagcgtt
     1921 tgaaacagcg gacagagtat gatatcgaaa tgttacgtga gatgggctat accaatgggg
     1981 ttgaaaatta ttctcgccac atggatggac ggagcgaagg agagcctcct tatacgcttc
     2041 tcgacttctt cccagatgat ttcttgatta tgattgacga gagtcatatg accatagggc
     2101 aaatcaaggg catgtacaat ggagaccgtt cgcgtaaaga aatgctggtt aattatggtt
     2161 tccgtttgcc gtctgctttg gacaatcgtc ctctccgtcg ggaggagttt gagagtcacg
     2221 ttcatcagat tgtttacgtt tcagcgacac ctggtgacta tgaaaatgaa cagaccgaga
     2281 cagtgattga gcaaatcatt cgtccaacgg gactcttgga tccagaggtg gaagtccgtc
     2341 cgactatggg acagattgat gacctcttgg gtgaaatcaa tgcccgcgtt gaaaaaaatg
     2401 agcgtacctt tatcacaact ttgaccaaga aaatggcaga ggatttgacc gactacttca
     2461 aggaaatggg tatcaaggtc aagtacatgc actcggatat caagaccttg gaacggacgg
     2521 agattatccg tgacctgcgc ttgggtgtct ttgatgtctt ggtcggaatt aacctgctcc
     2581 gtgaaggaat tgacgttcct gaagtgagcc tcgtagctat tctcgatgct gacaaggaag
     2641 gtttccttcg caacgaacgt ggactcatcc agactattgg acgtgctgct cgtaacagtg
     2701 aaggccatgt catcatgtat gcagacacgg ttacccagtc tatgcaacgt gctatcgatg
     2761 aaactgcccg ccgtcggaaa attcagatgg cctataatga agagcatggt atcgtaccac
     2821 agacaatcaa gaaagaaatc cgtgacctga ttgccgtgac caaggcagtt gctaaggaag
     2881 aggacaagga agtcgacatc aatagcctca acaaacaaga gcgcaaagaa ctcgtcaaga
     2941 aactagaaaa acaaatgcaa gaagccgtcg aagtgcttga ctttgaactg gcggctcaga
     3001 tccgtgatat gatgttggaa gtcaaggcct tggattaggg gaatagtatg atttatttaa
     3061 gaaagttaaa gagagaagat ttgatgtctt tatgggaaat ggcttattca caacttaatc
     3121 cagtttggaa acagtatgat gctccctatt atgatgatta tcagtatttt tcaaatttta
     3181 aagaattcga actacaaaaa tcagaatcca ttttaagcaa ctcaaatcgc cttggtattt
     3241 ttgttgatga taaactagtt gggactgttt cgcgttattg ggtatgtaaa caaacaagat
     3301 ggatggaatt gggaattggt atttatgata aaaaattctg gaacactggt attgggaaag
     3361 ttgctatgtt gcagtggata gataggacgt ttcaggatta cttggagttg gagcatctgg
     3421 gtttgacaac ttggtcaggg aatattggta tgatgaaact tgctgaaaaa ttaagaatga
     3481 aaaaagaagc tcatattcca aaagttcgtt attatcaagg taaatatttt gatagtatta
     3541 aatatggtat tttgagagaa gactgggaga aaataaatga cggttattat caaatcaatg
     3601 gaaactcctg aagagataga agataaatcc cttgttcact ggcaaacgtg gagagaggct
     3661 tatgatgacc ttttgcctgc ggaatttcag gatacaatga cattagaaag atgtcgattc
     3721 tttagtcaaa agtatccaga aaatacattg attgcgatgg atggcgtgaa ggtggttggt
     3781 tttatcagtt atggcaattt tcgtgatgag actattcaag ctggtgaaat tattgcttta
     3841 tatgttttaa aagactatta tggaaaaggt atcgcacaaa agttaatgaa agcagctttg
     3901 actgctctta atcatttttc tgaaattttc ttatgggtat tgaaagataa caagcgcgcc
     3961 attgctttct atcaaaaaat gggttttact tttgatggac aagaaaaaat acttgaactt
     4021 ggaaagccta taaagga
//
LOCUS       DAVLSL010000147         3951 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLSL010000147 DAVLSL010000000
VERSION     DAVLSL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.149"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            8..757
                     /locus_tag="VXN30_001825"
     CDS             8..757
                     /locus_tag="VXN30_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1358403.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSE"
     gene            827..1345
                     /gene="rimM"
                     /locus_tag="VXN30_001826"
     CDS             827..1345
                     /gene="rimM"
                     /locus_tag="VXN30_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1358404.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            1335..2054
                     /gene="trmD"
                     /locus_tag="VXN30_001827"
     CDS             1335..2054
                     /gene="trmD"
                     /locus_tag="VXN30_001827"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1358405.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            2066..2404
                     /locus_tag="VXN30_001828"
     CDS             2066..2404
                     /locus_tag="VXN30_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358406.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(2435..2755)
                     /locus_tag="VXN30_001829"
     CDS             complement(2435..2755)
                     /locus_tag="VXN30_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358407.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            2853..3068
                     /locus_tag="VXN30_001830"
     CDS             2853..3068
                     /locus_tag="VXN30_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1358408.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(3137..3217)
                     /locus_tag="VXN30_001831"
                     /pseudo
     CDS             complement(3137..3217)
                     /locus_tag="VXN30_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3273..3809)
                     /locus_tag="VXN30_001832"
     CDS             complement(3273..3809)
                     /locus_tag="VXN30_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1358409.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT         1294 a          682 c          854 g         1121 t
ORIGIN      
        1 tttctttatg aagaggtttg aagtatctac agaaattggt agcctctctg ttacttatca
       61 aaagcaaaag aaagtgctag tttgtctaaa cggagcaggt ttgctaccaa gttatgaaaa
      121 tttttcactt atacttgaaa aacctcctcc tacaattggt tatttgacaa ttgattttcc
      181 gaacacaggt aggagtccga ttcatgacca agctggaaaa aatctagata atcttgcaga
      241 tgcggtttat gaagtacttg aagaattagg aatttctgaa tatatacttt gtgcacatag
      301 ttggagtgga attttagctt gcaaattact cgagaaacca attaagcgtc agactttagt
      361 agcaattgaa ccgacaacta aaaaagtcat gtttgctgat ttttcagaaa atccttatcc
      421 agaaatggaa gagcagatga ggctgattga cgagtgtggt cctgaacttt attttaagaa
      481 cttaactcaa gcaacattta gtcctgaaac gaataaaaaa atctgggaat taatgcaaga
      541 aaaaggctta gagttggaaa atcaagatcc agaatttcag atatctggag agattactga
      601 ggaagatttt gagaatttgt cgatagaatc tcatgtccct gtatttattt tttgtcagac
      661 ttatagagaa aaagagtaca gagaatcaga atattggact tccaatacta aactcatttt
      721 aggaaggaat caccattatt tacaatggtc agaataggaa aaaattgcgg ctattattcg
      781 agaattgtca gaataagatg gaaaaaagga gattacagga gacaagatga actactttaa
      841 tgttgggaaa atcgttaata cgcagggatt acagggtgag atgcgagtct tgtctgtgac
      901 ggattttgca gaagaacggt ttaaaaaagg agctgagctg gctttgtttg atgaaaaaga
      961 tcagtttgtc caaacagtga ccatcgctag ccaccgtaaa cagaagaact ttgacattat
     1021 taaattcaaa gatatgtacc atatcaatac tatcgaaaag tacaagggat acagtctcaa
     1081 ggtcgctgag gaagatttga atgacctaga cgatggtgaa ttttactatc acgagattat
     1141 cggtttggaa gtctatgagg gtgatagctt ggttggaacc atcaaggaaa tcctgcaacc
     1201 aggtgctaat gatgtctggg tggtcaaacg aaaaggcaaa cgtgatttgc ttttacctta
     1261 tatcccacca gtggttctca atgttgatat tccaaataaa cgggtcgatg tggaaatctt
     1321 agaagggtta gacgatgaag attgatattt taaccctctt tccagagatg ttttctccac
     1381 tggagcactc aatcgttgga aaggctcgag aaaaagggct cttggatatc cagtatcata
     1441 attttcgaga aaatgctgaa aaggcccgtc atgtagatga tgagccctac ggaggcggtc
     1501 agggcatgtt gctcagagca caacctattt tcgattcctt tgatgctatt gaaaagaaaa
     1561 atccgcgcgt tattctcctc gatcctgctg gaaagcagtt tgatcaggct tatgctgaag
     1621 atttggctca agaggaagag ctaatcttta tctgtgggca ctatgagggt tatgatgagc
     1681 gcattaagac cttggtaaca gatgagattt ccctaggcga ctatgtcctc actggtggag
     1741 aattggcagc tatgaccatg attgatgcta cagttcgcct gattccagaa gtgattggca
     1801 aggagtctag ccaccaagat gatagttttt cttcaggtct tttagaatat cctcagtaca
     1861 cacgtcccta tgattatcga ggcatggtcg tgccagatgt attgatgagt ggccaccatg
     1921 aaaagattcg tcagtggcga ttgtacgaga gtttaaagaa aacctacgag cgcagaccgg
     1981 atttacttga acattatcaa ctgacagtag aagaagaaaa aatgctggca gaaatcaaag
     2041 aaaacaaaga ataaaggaga aacctatgca agtaatcaaa cgtaatggcg aaattgctga
     2101 atttaatcca gataagattt accaagccat cttgaaggca gcccaaactg tttatgtttt
     2161 gacagatgat ttgcgtcaaa atcttgctca agtcactaag aaggtagttt tggatttaca
     2221 agaagccaag gtggaacgtg cgactatcag tatgattcaa tctatggttg aacatcgttt
     2281 attgggcgca ggttacatta ccattgcaga acactacatt tcctatcgtt tacaacgtga
     2341 cttggaaaga agtggttatg gagatcatat tgcagttcat ttacattttg aacaaattcg
     2401 ctaagaaaaa agagtgggat gaagcaacta catctcactc tttcttaaat ctattttttt
     2461 gggctgtttg gacagttgag gggacaaatc gcaggcgttg aatatcgctt atacggataa
     2521 tccggctcac atttttggca aaatttttca cgataatttg ctggccttgc tgatcatatt
     2581 tgatgatatc acctgtaaaa cttgtctcag acaagataag gtgaacagcg gtttgtttct
     2641 ggatagcctc ttcaatagtg gctgtaaggg agttggtttc agtctggtca ggttggtcat
     2701 gtccatttaa aaaatcatgt attttatcta gaacttgctg gaatttatgt ctcatattgg
     2761 cctcccttct tttatatcaa tattatataa aattttccta aaatctacaa gattttacga
     2821 ataaactaat gaaactaaaa aaggagaaga aaatggcaga atttacattt gaaatcgaag
     2881 agcacttgtt gactctttct gaaaacgaaa aaggttggac caaggaaatt aaccgtgtga
     2941 gctttaatgg tgcccctgca aagtttgata ttcgtgcttg gagtccagac catactaaaa
     3001 tgggcaaagg gattactctc tcaaatgaag aatttcaaac gatggtggat gcctttaaag
     3061 gcaactaata ctcttcgaaa atctcttcaa accgcatcag ctttgccttg ccgtatgtat
     3121 ggttactgac ttcgtcagtt tcatccacaa cctcaaaaca gtgttttgag cagcctgcgg
     3181 ctagcttcct agtttgctct ttgattttca ttgagtataa gttttcaaaa taaaaaaaag
     3241 gatattgagt catgataact cggtatcctt ttttagttag taaagtaagc ctgattttta
     3301 aggcgttgaa gtaggggacg gctaataaaa ctaatagcca gaagtttgcc aaggtctgga
     3361 atgataaagg gaagaacccc cacaacaaga gctttttcaa atgccattcc agctaggaaa
     3421 tgcaagctga gaatcccgcc gacaaagaca agggcatcac ccaagaggtt tgcaagaaaa
     3481 atcttaacaa caccactctt gctgttggtt agagaggaag taagtccaga gtaaacgaga
     3541 taaaaccaaa gatagcctgc agtagggcca actaaagcct gaaaaccagc tccacctcct
     3601 gcaaagacag gaagaccgat agcacctaga agaagataga gtccagcaga aagtacagcc
     3661 tctctcggtc taaagacagt agcaatcaag ccgattgcaa agttttgcag agtgaaggga
     3721 acaggtccaa ttggaagact gatttgtgcc aaaacagcaa tgagagcagc cccaatagca
     3781 gggatagcat aaacgtgagc ttttttcaaa atagttaacc tcttttctaa tgtatagtaa
     3841 ctattatact catataggag tcgatgtcaa ccatttttta aaaacaaggt aacaaaaact
     3901 gtaacagccg attttcgtga ctagtcaagt tggataattt ttttgttgat t
//
LOCUS       DAVLSL010000148         3838 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLSL010000148 DAVLSL010000000
VERSION     DAVLSL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.150"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            69..521
                     /locus_tag="VXN30_001833"
     CDS             69..521
                     /locus_tag="VXN30_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358410.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYICRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1340..1699
                     /locus_tag="VXN30_001834"
     CDS             1340..1699
                     /locus_tag="VXN30_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358411.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1709..2074
                     /locus_tag="VXN30_001835"
     CDS             1709..2074
                     /locus_tag="VXN30_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1358412.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQNQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            2061..>3838
                     /locus_tag="VXN30_001836"
     CDS             2061..>3838
                     /locus_tag="VXN30_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1358413.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKTYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYEE"
BASE COUNT         1382 a          589 c          771 g         1096 t
ORIGIN      
        1 ttgaaaaaag gttctcactt atcaggtgca tataaacgaa aaattttttc taaagaaaat
       61 acacgatttt ggagtttaat attaactcga aagaatcttc gcaacaactt gatattctct
      121 ataaagaagt tggtgtatta agtttgggac aaaaagttgt agccatgctt gattttttat
      181 tagcatatag tgattattct aaagacttca gaccattgat tattgatcag cctgaagaca
      241 atctagacaa tcgttatatt tgcaggcatt tagttcagca gtttagagat gtgaaagctc
      301 aacgtcaaat tattttagca acacataatg ctacaattgt aacaaattct atgacagatc
      361 aagttgttat tatggagtca gatggagtta acggatggat tgaatcacag ggatatgtta
      421 gtgaaaaata tataaaaaat catatcatca atcaattaga gggaggaaaa gattccttca
      481 agcataaaat gtctatatat gagacggctt tatcagagta gagtcagaaa aagtaggtta
      541 gaaatttagc ctactttttt ctttgtccga caggcatagt gtgcatctga ggtccaagtc
      601 ctctgtggat atttgctgca gatgaaacca atagcgactc ctaagcctga atatcgtgag
      661 gtagggggga taggaaggaa ttagcgaaat caaggttcta caaacagaat cgtgacttga
      721 agccatatat agcggatgag gaactctaaa atccaaatag gtgtcgtaac ctatatacgt
      781 aaattacgag agtaaactag gaaagatgta cggcttattc cgtgagcgtt taggacgtag
      841 tacaacgaat catgggagtc agctgaacac atagtattga agaaatttct gtaatggaaa
      901 tggagcgaag aagtgaacaa ttaaatgaat acctctctaa ttaaatttgt caattctaat
      961 tcctggtatg aaaagacagt gacctgaaaa tgtaaacgat gggagctgat tataaatata
     1021 ggacggtaca tgcagtggtg ttagagatta gtccctactt gatttgtgat aacttcccca
     1081 aatttcttct gctatacttt tctcaacttt taaaaatcca actaagaatt ttacctgggg
     1141 gtttgggggc gaagccacca agttatctta tcgttggctg tcaaaactgg aaggttttgg
     1201 ttagctggcg atatgatttt ttgggtattg taaccacaat gcttgagctc gcaaagaccg
     1261 aacaaggact ttaagaaatt tcccgtatga cacacggggt cggggtttac ccgagaaagg
     1321 atgaaagtga ggaaaagaga tgtctgaaca gtaccgtgac attcgaaaag aggtaaatct
     1381 aaccgcagat gagctaaaac agattgaaaa aatgatggag gttgataatt atcgtcactt
     1441 ttcaccattc gtcagggata aaattttgat gactgatgac aaacagttag ctgccaaaga
     1501 atggttttcc ctttggcagt ctcaaaagtt tgaacagatc agtcgagatg tgcatttggt
     1561 tttgattatc gcaagagaga atcaccaagt gactcaagaa cacgtgtcaa tcttattgac
     1621 ctgcgttcaa gaattgattg cggaagtcaa tcaagtacag tcactcagtc gtgggtttcg
     1681 tgaaaaatac atgagatagg aggctactat ggtttatcgt tatcgtacca atctcaaaaa
     1741 agtatttcta acagattcag aattacacca attgaatgaa cggattgcta agagcaattg
     1801 tcaaaatttc tcagtctatg ccagaaaagt tttgcttaat cccaatatgt catttgtcac
     1861 gattaacact gatacttatg aacagttagt gtttgaattg aggaggattg gaaataacat
     1921 caatcaaatt gcgcgtgcga ttaatcaaag ccatctgatt tctcagaacc aattacaaga
     1981 attgagtaaa ggagtcggag agttaattaa ggaagtggac aaagaatttc aagtggaggt
     2041 gaaaagactg aaggagtttc atggtagtta ctaaacactt tgcgacgcac ggaaaaaaat
     2101 atcgtaggcg tttaattaag tatattctca accctgataa aacggacaat ttgaaattgg
     2161 tatctgattt tggcatgagc aattacttgg acttccctag tcatgcagaa atggtagaga
     2221 tgtacaatgt caacttcagc aataatgaca agttgtacga atccagaaac gaccgacaag
     2281 aaaaacacca acaaactatt catgctcatt acctcattca atcattttct cctgaagata
     2341 atctgacacc tgaagaaatt aaccgcattg gttatgagat catgatggaa ttgacaggag
     2401 gtcgttttaa gtttatcgta gcaactcaca cagacaaaga tcatgttcat aatcacatcc
     2461 taatcaacgc tattgaccgc aattcagata aaaagttgat atggaactat gccttggaac
     2521 gaaacctccg tatgatttca gaccgtattt ctaaagtgac tggtgcaaaa attattgaga
     2581 aacgttattc ctatcgagat tataaaaaat atagggaatc tagtcataag tttgaattga
     2641 aacaacgtct ttattttttg ctacagcaat ctaaatcgtt tgatgatttt ttagagaaag
     2701 caaagcagtt gcatgttcag attgatttta gtcaaaagca tagccgtttt ttgatgacag
     2761 atagaacgat gataaaacca attcgaggac gccaactcag caaacgagat ttatatgatg
     2821 aagacttttt taaaacatat tttgccaaac aagagattga aagtcgttta aaatttttac
     2881 taaagtctgt ttattcatta gaagaattgc atgtaaaggc taaagagttg aatctaacca
     2941 tcgagttaaa acagaaaaat gtgatgttta ccctagaaga agatggcaaa aagataagcc
     3001 tgagtcataa aaaaattagc gataagaaat tgtacgatgt tcaatttttt aatcgttatt
     3061 ttgaagatag agaagttaga gatattcaag cattagaaaa tcttcaggaa gattttcaga
     3121 cttttcgaga ggagcagcat aaggagaaag tctccgctga agaaatcgag gaagccttca
     3181 agaaatacaa ggaaaaacga gatgctatcc atgagtttga gattgaactt acggataatc
     3241 agatagagaa gttggttgat gatggtgtct atatacaggt ttcgtttggt atcaaacaga
     3301 gtggactcat ctttattcct aactaccagc tagatatttt tgaggaagac aatcataaaa
     3361 aatataaagt ctacattcga gaaacgtctt cttactttgt ctataataaa gaaaacatgg
     3421 ataataattg tttcatcaag ggtaggacct taatcagaca attgagcaac gatagccaaa
     3481 aactccccta tagacgacca actttaaaaa gtcttaaaga gaagatttct gaaattaatc
     3541 ttatgattga actatctaac acgaacaaac aataccaaga aatcaaagat gagcttgtat
     3601 tagaaattgc agagattgat atgcagctag aagaaaccca agaaaaaatc gccaccttaa
     3661 acaagatggc ggaagtcctt atcaatttga agagtgaaga tcatgagact agaaaattag
     3721 ctaagtatga ttttgaccaa atgaacatga cggaatcaat aatgttagat cgactcaata
     3781 cggatatttt aaaattacaa caggaacttg gtaatgaaat taataaatat gaggagat
//
LOCUS       DAVLSL010000149         3834 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLSL010000149 DAVLSL010000000
VERSION     DAVLSL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.151"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            2..481
                     /gene="rlmH"
                     /locus_tag="VXN30_001837"
     CDS             2..481
                     /gene="rlmH"
                     /locus_tag="VXN30_001837"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1358414.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            525..598
                     /locus_tag="VXN30_001838"
     tRNA            525..598
                     /locus_tag="VXN30_001838"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:559..561,aa:Arg,seq:tct)
     gene            763..888
                     /gene="comC"
                     /locus_tag="VXN30_001839"
     CDS             763..888
                     /gene="comC"
                     /locus_tag="VXN30_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1358415.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIKGGEMRISRIILDFLFLRKK"
     gene            909..2234
                     /locus_tag="VXN30_001840"
     CDS             909..2234
                     /locus_tag="VXN30_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1358416.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYIFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYFYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            2231..2983
                     /locus_tag="VXN30_001841"
     CDS             2231..2983
                     /locus_tag="VXN30_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1358417.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            3035..3106
                     /locus_tag="VXN30_001842"
     tRNA            3035..3106
                     /locus_tag="VXN30_001842"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3068..3070,aa:Glu,seq:ttc)
     gene            3112..3185
                     /locus_tag="VXN30_001843"
     tRNA            3112..3185
                     /locus_tag="VXN30_001843"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3146..3148,aa:Asn,seq:gtt)
     gene            complement(3226..3768)
                     /locus_tag="VXN30_001844"
     CDS             complement(3226..3768)
                     /locus_tag="VXN30_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1358418.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
BASE COUNT         1277 a          536 c          716 g         1305 t
ORIGIN      
        1 catgaaaatt aaagttgtaa cagttgggaa actgaaagaa aagtatttaa aagatggtat
       61 cgcagagtat tcaaaacgaa tttctagatt tgctaagttt gaaatgattg agttatcaga
      121 tgaaaaaaca ccagataagg ccagtgaatc agaaaatcaa aagattttag aaatagaagg
      181 tcagagaatt ttatcaaaaa ttgctgaccg tgatttcgtt attgtgttag ccattgaagg
      241 gaaaactttc ttctcagaag aatttagtaa gcagttagaa gaaacttcta taaaaggatt
      301 ttctactctt acttttatta ttgggggaag tttaggattg tcatcatctg taaaaaatag
      361 agccaatctt tctgtcagtt ttggtcgcct aaccttgcct catcagttaa tgagactagt
      421 tcttgttgaa caaatctatc gcgcttttac gattcagcag ggattcccct accataaata
      481 gaaaattgac ttttaattga atttttggta gaataattgt gttaggtctc atagctcagc
      541 tggatagagc attcgccttc taagcgaacg gtcgcaggtt cgaatcctgc tgggatcaat
      601 ataatagcaa agctgggaat tttcccggct tttttcttaa aaaagtacac tttgggagaa
      661 aaaaatgaca gttgagagaa ttttatctaa agtgaaattc cattttgtat aatggttttt
      721 gtaagttagc ttacaagaaa aaacatttta ggagatttta ttatgaaaaa cacagttaaa
      781 ttggaacagt ttgtagcttt gaaggaaaaa gacttacaaa atattaaagg tggggagatg
      841 aggatttcaa gaatcatcct tgattttctt ttcctacgaa aaaagtaatg aaataaggag
      901 aaagagtaat ggatttactt ggatttggga cagttattgt tcatttttta attattagtc
      961 acagttaccg tttaatttgt aaaggtcgaa taaatagaaa agaattatac atttttggtg
     1021 cttatacatt actaactgaa atagtacttg aattttcctt ttatcttcta tatttagata
     1081 aaatagggat tgaaagattt ttatttcctt tgggcttata ttcctatttt cgatggatga
     1141 aacagtatga gagggataga ggactattcc taagtttact actatctctt ttatatgaga
     1201 gcactcataa ctttctgtcc gtaactttct cctctataac aggagataat tttgttttac
     1261 aatatcattt cccattcttt ttcgttgtga cggtgttaac ctattttgtt acattaaaaa
     1321 tcatttatta tttccatttg gaactagcct attttgacaa ggactacttt tatcctttct
     1381 tgaaaaaagt attttttgct ttactattgc tacatattgt atctttcgtt tcagatatgg
     1441 taagtacgat taaacatttg aatagttttg gaagtatttt gtcatctatt gtctttatct
     1501 ctctcctttt gaccttcttt gcaatgaatt ctcataaagt tcaaatggag aaagagattg
     1561 ctttgaagca gaagaaattt gaacagaaac atttacagaa ttacacagat gaaattgttg
     1621 gtctgtataa tgaaatccgt ggttttcgac atgattatgc tggaatgctt gtcagcatgc
     1681 agatggcaat tgacagtggt aatttacagg aaattgacag aatttacaat gaagttttag
     1741 tcaaagcaaa tcataaattg cgttcagata agtacactta ctttgatttg aacaacatag
     1801 aagactcagc tttacgaagt ttggttgctc agtcaattgt ctatgctcga aataatggtg
     1861 tagagtttac actggaagta aaagatacga ttaccaagct tccaattgaa ctattggatt
     1921 tggttcgtat catgagcgtt ttattgaata atgctgtcga aggatcggct gatagctata
     1981 aaaagcagat ggaagtagca gttattaaga tggaaactga aacagttatt gtgattcaga
     2041 attcatgtaa aatgacgatg actccttcag gagatctatt tgccttagga ttctccacta
     2101 agggaagaaa tcgcggagtc ggattaaata atgtgaaaga actactagat aagtacaaca
     2161 atattatttt agaaacagag atggaaggca gtacatttag acaaatcatt agatttaaga
     2221 gggaatttga atgaaagttt taattttaga agatgttatt gaacatcaag tgagactaga
     2281 gagaatattg gatgaaattt cgaaagaatc gaatattcca atatcataca agacaacggg
     2341 aaaagtccgt gaatttgaag aatacattga aaatgatgaa gtaaatcagc tttatttcct
     2401 agatatcgat attcatggaa ttgagaaaaa gggatttgaa gtggctcagc tcattcgtca
     2461 ttacaatcct tacgctatta tcgtctttat cactagtcga tcagagtttg cgactctaac
     2521 ctataaatac caggtatcag ccctagattt tgttgataag gatatcaatg atgagatgtt
     2581 taagaagaga attgagcaaa atatcttcta cacgaagagt atgttacttg aaaatgaaga
     2641 tgttgtagat tatttcgact acaattacaa gggaaatgat ttaaaaattc cttaccatga
     2701 tattttgtat attgaaacaa caggggtatc tcataaattg cgcattattg gtaagaattt
     2761 tgcaaaagag ttttatggta ccatgacaga tattcaggaa aaggacaaac atactcagcg
     2821 attttattct cctcacaagt catttttggt aaatataggc aatatcagag aaattgatcg
     2881 aaaaaactta gaaattgttt tctatgaaga ccatcgttgt cctatttcaa gattaaaaat
     2941 tagaaaatta aaagatattt tagagaaaaa atctcaaaag tgattgacaa ttagcaagaa
     3001 attgatataa tggttatatc tgctacagat agaaggtccg ttggtcaagg ggttaagaca
     3061 ccgccttttc acggcggtaa cacgggttcg aatcccgtac ggactatttt atccgccata
     3121 gctcagttgg tagtagcgca tgactgttaa tcatgatgtc gtaggttcga gtcctactgg
     3181 cggagtatag ataaaaggga acacagctgt gttcctcttt ttgtatcaat ttgtatcacc
     3241 aagcattttc ataaggaagt ctgttatttc ttgagaactt tctttttttc catgtgcaat
     3301 ccaagtttgg cagacaccaa aaagtgcatg agttagatag atgctactat attctaattc
     3361 ggtggtattt agattcagtt gcataaatcg cttttgtaaa tctgtactaa gcatgatatg
     3421 aagtttattt cgtaagaaat tttggatttc tttagtccca ttttcagaaa gaagggcagc
     3481 cagaagtggt tctgactcta gatattcaaa aacttctaaa atagcgtctc ttttgtgatg
     3541 agcatgtttt tgaaaaatat attcaaatgt atggaatagc ttgctttgat agtgctcaat
     3601 catatcatac ttatccttat agtgagtata gaagctggaa cgactaattc cggctttttc
     3661 tactaatttg acagtagaaa ttttatcaaa tggctgttcc atcagtaatt gtaccatagc
     3721 attttcaata gttcgctttg tttttaagcg tttgttactt tcttgcatat ttcctccttg
     3781 taaacaaatt agactatatg tctaaaaata gattttttat cttgtaattt agat
//
LOCUS       DAVLSL010000150         3784 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLSL010000150 DAVLSL010000000
VERSION     DAVLSL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.152"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..1627
                     /locus_tag="VXN30_001845"
     CDS             <1..1627
                     /locus_tag="VXN30_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEV1358419.1"
                     /translation="AKVFANENLIKNSNFEQGELFWNSRNRGIINSNESYSNGHYSGM
                     IPSTAINGNRADGYIGQVIDIEPNEDYLVSAFAKVDVSGAEGYFTARWFDDNKQGEIV
                     QNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVKWSEDDTTKKSNIFIDNVEMY
                     QLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQQHYVRSDENVFLRSGNLVLR
                     ATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYGKLEMRAKLPKGQGVFPAFWT
                     LGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNGSGNKTVYQTLHYGQSDNDDG
                     KFAGHGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDNHIVRKVYYGSDNIAKKIFNR
                     PQYVQLNLAMGGNWPGVVDNNLSGTEFVVDYVSYSRNDLQQHQAEEYYSQSPKINGAK
                     DIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFGGNTSVDLLIRDKTEKEKISQ
                     MPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            1640..1957
                     /locus_tag="VXN30_001846"
     CDS             1640..1957
                     /locus_tag="VXN30_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1358420.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            1974..2291
                     /locus_tag="VXN30_001847"
     CDS             1974..2291
                     /locus_tag="VXN30_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1358421.1"
                     /translation="MNESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            2301..3656
                     /locus_tag="VXN30_001848"
     CDS             2301..3656
                     /locus_tag="VXN30_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1358422.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
BASE COUNT         1234 a          499 c          823 g         1228 t
ORIGIN      
        1 tgcgaaagtt tttgctaatg aaaatttaat aaaaaattca aattttgagc aaggagagct
       61 gttttggaac tctagaaata ggggaataat taattcaaat gagagttata gtaacggaca
      121 ttactcaggt atgattcctt ctactgcaat taatggtaat agagcagatg gatatattgg
      181 acaagttatt gatatagaac ctaatgaaga ttatttagta tctgcttttg ccaaagtaga
      241 tgttagtgga gcagagggat attttaccgc aagatggttt gatgataata agcaaggaga
      301 gattgttcaa aataacattg gagaagctgt tgatcaaaca gttaatacaa ccgaatggaa
      361 aaaatacatg ttttctttca attccggtaa acacgataaa gttttgattc aattagtgaa
      421 atggtcagaa gacgatacta caaaaaaatc taatattttt attgataatg tagagatgta
      481 tcaactgtct aaaggaaatt catataagaa aatttggaga gatgattttg atggcgaaca
      541 attaaataaa aaatattggg gatatgaatt aggatcaatt cgtggttggg aacagcaaca
      601 ttatgtaaga agtgatgaga atgtttttct acgttcggga aatttagttc ttagagctac
      661 gaatcgctct aaggaagatc agtattttaa cccaagaaac aatcacagaa aggttgtata
      721 taattcagga agtgttagaa cacatgggaa agtagaattt ctttatggta aattagagat
      781 gcgtgcaaaa ttaccgaaag gtcaaggagt ttttcctgct ttctggactc ttggatcgga
      841 ttttacactt gatgggaaga ttaatcctgt tcagggacga ggatggccgt caacaggtga
      901 gatagatata atggaattag ttggtgaacg aaattcaaat ggaagtggga ataagacagt
      961 ttatcaaacg cttcattatg gacagtcgga taatgatgat ggaaaatttg caggacatgg
     1021 tacagccttt aaattatcga gtggaaactt taatgattcg tatcacactt tttcaattga
     1081 ttggtataaa gattatattg tatggatggt tgacaatcat attgtcagaa aagtatatta
     1141 tggatcagat aatatagcga aaaagatttt taatagacct cagtatgttc agttaaattt
     1201 agcgatggga ggaaattggc ccggagtagt ggacaataat ttatcaggaa cagaatttgt
     1261 agttgattat gtttcttact ctagaaatga tttacaacaa catcaagctg aagagtatta
     1321 tagtcaatct cctaaaataa atggcgctaa agatatagta ataaacagag gagatgttcc
     1381 tgatttatta aaaggaatta caactaatag tggatatgag ctagattatt ctattgagaa
     1441 tgaacctatg tttaataact ttggagggaa tacttcggta gatttattaa ttagagataa
     1501 aactgaaaag gaaaagattt cacaaatgcc tccaggagtt tataatttgc attatactag
     1561 tcgtcccaaa catttagatt atgagtctaa agttgatcga aaaacagtaa cattgacaat
     1621 aaaataaaag gagtaagcta tggctaaagt aacaattatg ttagcatgtg cagcaggtat
     1681 gagtacaagt ctgctagtga caaagatgca aaaggcagca gaagataagg ggttggatgc
     1741 agaaattttt gcagttccag ctcctgaagc agaagaaatt gtagcaacaa aagaagtaaa
     1801 tgtgttgctt ttaggtcctc aagttcgcta tttactaggg gactttcaag aaaaactaaa
     1861 agatagacag attcctgtgg cggttattcc gatgacagat tacggaatga tgaatggttc
     1921 taaagtttta gatttagctg aaagtttatt agactaagat tgaggagaat gctatgaatg
     1981 aaagtaattt agaatctgta atggggctaa ttatgtatgg tggggaagcc aaaagtaatg
     2041 ctatggaggc tattcaggca gcaaaaaaag gggatttctc taaagccaat cgaagattag
     2101 ctgatgcgaa tgctgcctta ttacaggcgc ataaggctca aacagaaatg ttgacaaaag
     2161 aggcacaggg ggaagaaaca tcaattagcc tcttgatggt acacgcgcaa gatcatttaa
     2221 tgacgagtct gacttttgtt gacttggcga aagaagtggt agaagtatac gaacgatttg
     2281 aaaaaaatta ggagtttggt atgaatacaa tgttggataa aatgcaagaa aaactttctc
     2341 caattgcaat gaaagttgga aatcagaagt ttttagttgc tttacgtgat tcatttgtgg
     2401 ggactatgcc tgttattatg acaggttcca ttgctttgtt attaaatgct tttctagtgg
     2461 atttaccaca acaatttcac ctagaaagta ttacgaaaac ctttcagtgg cttgttgaca
     2521 tcaataattt ggtattcaag ggaagtatac caattgtttc cttgttgttc atctattgct
     2581 tgggagtgaa tatcgctaag atttacaagg ttgatacagt ttctgctggt ttggtatcac
     2641 ttgcttcgtt tgtcatttcg attggaagta cagttacaaa gagtttccct ttggcaaatg
     2701 taggagatgt gaaattagat cagatcttac aaggaattga taatctagca tttgatggta
     2761 aaaatctgat ggtaactatt gggaatgtga ttccaggaaa ccatattaat gccagaggct
     2821 actttacagc catgatgatt ggatttttag cctctattat tttctgtaaa gtaatgaaaa
     2881 agaactgggt aatcaagtta ccagattcag ttccccctgc gattgctaag ccgttcactt
     2941 ctatcattcc aggatttatg gcgatgtata tagtagccat cttgacttat gttttccatt
     3001 tacttagcaa tgacttattg attgattggg tttacaaggt gttacaaacg ccattactag
     3061 gtttgtctca aagtttcttt gcagttattc tgatgatctt tttgaataag ttattctggt
     3121 tttttggtct ccatggagga aatgtattgg ctcccatcat ggaagggctg tttggagttg
     3181 ctatgttagc gaatctggat gctttccaaa aaggtgaacc gattccttat atatggacaa
     3241 gtgggtcttt tggggcgttt gtctggtttg gaggattagg attggtactt gctattttaa
     3301 ttttttcaag aaacagtcat tatcgaaaag ttgctaaact tggtctagca ccagttctgt
     3361 ttaatattgg tgagccggtc aattatggtt taccagttgt cttgaatcct ttgctattta
     3421 taccatttgt acttagcccg gttttcatgg caacggtagc ttactgggca acaagctggg
     3481 gtcttgtttc acctgttaca caaaatgtta cttgggtaat gcctccaatt ttatatggtt
     3541 tcttctctac agcatttgat tggcgtgcca tcatcttatc agttgtttgt ttgattatta
     3601 gtgtcttgac ttatttccca tttgtgaaaa tggcagataa aactgaattg agttaatggt
     3661 cttacttgcg ttttttgtga taaaatagaa atagattacg tgtaagatga gttaggtgta
     3721 ggatttctta tctccactaa aattgttatt agacaaaggt ttatagttta ttgaagttgg
     3781 aata
//
LOCUS       DAVLSL010000151         3739 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLSL010000151 DAVLSL010000000
VERSION     DAVLSL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.153"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            71..1252
                     /locus_tag="VXN30_001849"
     CDS             71..1252
                     /locus_tag="VXN30_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1358423.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1310..2068
                     /locus_tag="VXN30_001850"
     CDS             1310..2068
                     /locus_tag="VXN30_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1358424.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            2281..3642
                     /gene="dnaA"
                     /locus_tag="VXN30_001851"
     CDS             2281..3642
                     /gene="dnaA"
                     /locus_tag="VXN30_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1358425.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1328 a          647 c          670 g         1094 t
ORIGIN      
        1 cttttgtgtt taagttataa ttaagctagt cagtttatac tttcagtaat tcaaacatat
       61 ggaggcaaat atgaaacatc taaaaacatt ttacaaaaaa tggtttcaat tattagtcgt
      121 tatcgtcatt agctttttta gtggagcctt gggtagtttt tcaataactc aactaactca
      181 aaaaagtagt gtaaacaact ctaacaataa tagtactatt acacaaactg cctataagaa
      241 cgaaaattca acaacacagg ctgttaacaa agtaaaagat gctgttgttt ctgttattac
      301 ttattcggca aacagacaaa atagcgtatt tggcaatgat gatactgaca cagattctca
      361 acgaatctct agtgaaggat ctggagttat ttataaaaag aatgataaag aagcttacat
      421 cgtcaccaac aatcacgtta taaatggcgc cagcaaagta gatattcgat tgtcagatgg
      481 gactaaagta cctggagaaa ttgtcggagc tgacactttc tctgatattg ctgtcgtaaa
      541 aatctcttca gaaaaagtga caacagtagc tgagtttggt gattctagta agttaactgt
      601 aggagaaact gctattgcca tcggtagccc gttaggttct gaatatgcaa atactgtcac
      661 tcaaggtatc gtatccagtc tcaatagaaa tgtatcctta aaatcggaag atggacaagc
      721 tatttctaca aaagccatcc aaactgatac tgctattaac ccaggtaact ctggcggccc
      781 actgatcaat attcaagggc aggttatcgg aattacctca agtaaaattg ctacaaatgg
      841 aggaacatct gtagaaggtc ttggtttcgc aattcctgca aatgatgcta tcaatattat
      901 tgaacagtta gaaaaaaacg gaaaagtgac gcgtccagct ttgggaatcc agatggttaa
      961 tttatctaat gtgagtacaa gcgacatcag aagactcaat attccaagta atgttacatc
     1021 tggtgtagtt gttcgttcgg tacaaagtaa tatgcctgcc aatggtcacc ttgaaaaata
     1081 cgatgtaatt acaaaagtag atgacaaaga gattgcttca tcaacagact tacaaagtgc
     1141 tctttacaac cattctatcg gagacaccat taagataacc tactatcgta acgggaaaga
     1201 agaaactacc tctatcaaac ttaacaagag ttcaggtgat ttagaatctt aattgacatc
     1261 tatgtaaaga aagctttaca taagagaaaa gatgtgttag tgtagaatca tggaaaaatt
     1321 tgaaatgatt tctatcacag atatacaaaa aaatccctat caaccccgaa aagaatttga
     1381 tagagaaaag ctagatgaac tagcacagtc tatcaaagaa aatggggtca ttcaaccgat
     1441 tattgttcgt caatctcctg ttattggtta tgaaatcctt gcaggagaga gacgctatcg
     1501 ggcttcactt ttagctggtc tacggtctat cccagctgtt gttaaacaga tttcagacca
     1561 agagatgatg gtccagtcca ttattgaaaa tttacagaga gaaaatttaa acccaataga
     1621 agaagcacgc gcctatgaat ctctcgtaga gaaaggattc acccatgctg aaattgcaga
     1681 taagatgggc aagtctcgtc catatatcag caactccatt cgtttacttt ccttgccaga
     1741 acagattctc tcagaagtag aaaatggcaa actatcacaa gcccatgcgc gttccctagt
     1801 tgggttaaat aaggaacaac aagactattt ctttcaacgg attatagaag aagatatttc
     1861 tgtaaggaag ttagaaactc ttctgacaga gaaaaaacaa aagaaacagc aaaaaactaa
     1921 tcatttcata caaaatgaag aaaaacagtt aagaaaacta ctcggattag atgtagaaat
     1981 taaactatct aaaaaagaca gtggaaaaat cattatttct ttttcaaatc aagaagaata
     2041 tagtagaatt atcaacagcc tgaaataagg ctgttctttt atttttttat ctcacaaggt
     2101 tatccactat gtttttcgat aaaaagctta ataaatcaat aatttcttct tttatcccca
     2161 acctgtggat aaagtttggt aacattgtgg attatttttc acagcttgtg gaaaattctt
     2221 gctatctatg gtaaaatatc tctagtatta aacttttaaa tagtaaagga ggagaaagga
     2281 ttgaaagaaa aacaattttg gaatcgtata ttagaatttg cacaagaaag actgactcga
     2341 tccatgtatg atttctatgc tattcaagct gaactcatca aggtagagga aaatgttgcc
     2401 actatatttc tacctcgctc tgaaatggaa atggtctggg aaaaacaact aaaagatatt
     2461 attgtagtag ctggttttga aatttatgac gctgaaataa ctccccacta tattttcacc
     2521 aaacctcaag atacgactag ctcacaagtt gaagaagcta caaatttaac tctttatgac
     2581 tatagtccaa agttagtatc tattccttat tcagatacgg gattaaaaga aaagtatacc
     2641 tttgataact ttattcaagg ggatggaaat gtttgggctg tatcagccgc tttagctgtc
     2701 tctgaagatt tggctctgac ctataaccct ctttttatct atggaggacc aggccttggt
     2761 aagactcact tattaaacgc tattggaaat gaaattctaa aaaatattcc taatgcgcgt
     2821 gttaaatata tccctgccga aagctttatt aatgactttc ttgatcacct aagacttggg
     2881 gaaatggaaa agtttaaaaa gacctatcgt agtcttgatc ttttgttaat cgatgatata
     2941 cagtcactca gcggaaaaaa agtcgcaact caggaagaat ttttcaatac ctttaacgcc
     3001 cttcatgaca agcaaaaaca gattgtccta acgagtgatc gtagtccaaa acatctagaa
     3061 gggctcgagg agaggcttgt cacgcgtttt agttggggat tgacacaaac tatcacaccc
     3121 cctgactttg aaacacgtat tgccatttta caaagtaaga cggaacattt aggctacaat
     3181 ttccaaagtg atactctaga atacctagct gggcaatttg attcaaatgt tcgagatctt
     3241 gagggagcca tcaacgacat cactttaatt gccagagtaa aaaaaatcaa ggatatcact
     3301 attgatattg ctgcagaagc cattagagcc cgcaaacaag atgttagcca aatgctcgtc
     3361 atcccaattg ataaaatcca aactgaagtt ggtaactttt atggtgttag tatcaaagaa
     3421 atgaagggaa gtagacgcct tcaaaatatt gttttggccc gtcaagtagc catgtattta
     3481 tctagagaac taacagataa tagtcttcca aaaattggga aggaatttgg gggaaaagat
     3541 cataccacag tcattcatgc ccatgccaaa ataaaatctt tgattgatca agacgataat
     3601 ttacgtttag aaattgaatc aatcaaaaag aaaatcaaat aatttgtgga taacttttag
     3661 ttttttatct tttttatcca cattttttaa acaagctaaa aaacttgata tgacttgttt
     3721 aaaggctgtt ttccacaga
//
LOCUS       DAVLSL010000152         3721 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLSL010000152 DAVLSL010000000
VERSION     DAVLSL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.154"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..1620)
                     /locus_tag="VXN30_001852"
     CDS             complement(<1..1620)
                     /locus_tag="VXN30_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEV1358426.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKYLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKKMLEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTAVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDIQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMNHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEE"
     gene            complement(1626..2945)
                     /locus_tag="VXN30_001853"
     CDS             complement(1626..2945)
                     /locus_tag="VXN30_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1358427.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYIVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            complement(2942..>3721)
                     /locus_tag="VXN30_001854"
     CDS             complement(2942..>3721)
                     /locus_tag="VXN30_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1358428.1"
                     /translation="LLTILISIFPIAWIFLSSLKADPMKNPGISLPTDFTLEGYINVF
                     TKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARMEFRGKKLVTSMLYSTLFIPAT
                     AMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFIIKNYFEIIPKELEEAAEIDGA
                     TYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASMLVIDKNELTVPALLGQFTTS
                     FNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGAVKG"
BASE COUNT         1238 a          770 c          494 g         1219 t
ORIGIN      
        1 ctcctctatc catttgacgg cacgagcatt ggctttccag cgttcttcat caaacatatc
       61 aaaagaatag tgaaactccc aaaaagcatt ccctcttgtt gaaataaata atagataagc
      121 ttcaaaatct ggtattgatg caaacatttg atgattcatg taccaggtat ttgcactgac
      181 agcatagatt ggttcatgat tataaagcga ccacataggt aactgtatct cacgtttttc
      241 caaaaattct tgatactgaa tatctcggta agttatcata cgattgatat cattacctgc
      301 attctctgta aagcctacat cttgagatat ttgaatccat aaactattga cccactgtaa
      361 aaaccatgga ctaggattta cataagaagt caagtttaac caacaatctt ttcctcctct
      421 ctcctttctt agatctatca acagttgaat taagaactca taaaccgctg tcatggtata
      481 cataccgtgc ggtccactct tatcaggttt gtcaggttga agtaaccaac catcaatttt
      541 ccaatagctg atatcgaatt ctttttgata ttccaacatc tttttcttca tttgattgag
      601 atagttaaaa tcagccacgt ttacatcatt tgaaatcaaa tttttagatc caatatttaa
      661 ctctggatgt gcttctaacc aatcactcat aatgatttct gtcccattgt aaccacctcg
      721 gggaccaatc cacaatccta ggcttgaacc aaatccattt acaagatatt taatatttct
      781 caaaccattt gggaatttat gattgaattc ccaaactgat tgatagtttg tccaaccatc
      841 atcaacaaca taagcatcta aatgaactcc atgattttca aatccatctc gaatctcaga
      901 aaaacttttt aaaatacctt cctctgtaat atcggtcata tggtcatacc aggaattata
      961 ctgtttacgg aaataactag gttgagctat tccttcaaca tattcaaaaa atgcctcttg
     1021 aatctcttct tttttataag aacatgctcc cccaataata caagaccaca aactcttttc
     1081 ttgatttaca acagttccca agtaatatct tgatacatag tgtctaccat ctaccttgtt
     1141 ttcagacatt ggaaattcca ttcctaagaa taaagaattc gcataaacag gttgtcccaa
     1201 ttctacataa taaccggaaa aattcgccat ttccttaata caatcttgtc ttttcggata
     1261 gaaaatattg tcttctgttt caaactcaaa actctctacc tctacagatt taataacctc
     1321 agtagatgat tctaaaacta attgcttcga aagaacatca tccctcgatt caaagattaa
     1381 acgtaaacga atggaaggac aaacaaaaga aacctgaatt cgcgtatctc cctcttccct
     1441 aacgattgct gaaaaatgat ttgaagaaag tcttatacca ttctccaatg taatggtaaa
     1501 ttcttctttc gaaacatttt ttatcattac tcctgcccgt ttgtttacga tatcagtagt
     1561 ataaaatcga ccatctcccc aaaagaaatt acgtcttaca tttttatttt caattttcat
     1621 ataaactact ctctcaactc aattttgatt acgctatcaa tcaagtctgg taatggatag
     1681 gtaaaatgtg gaacttcccc aaactgtgca aaacaaattc ctttgtaggc attggtcgtc
     1741 cagctttctg aaattttcac ctcacttcca tcatgaagaa agctcattct ttttacgttt
     1801 tctttactaa taccaagaag agctaaagga cctataggtt gttcaaatac atgagcataa
     1861 accgtatttc cattacgagt atagtatccc caatctggtt ttggtaaatt ttcatctcca
     1921 ctacatccat aaattgcctc accatttcga gacatccatc gatgaaaatt atctaatatt
     1981 ttcttacttg aatcattaat tcttcccaaa gcatctgggc ctacattaag aatcatattt
     2041 ccgtttttac taacacattc cactaacttt cttatcaaag tttgactaga cttatataga
     2101 taatctgtag gattataagc ccaattgtta ttcatagtta gacaaagttc ccaaggaaca
     2161 ggttcacctt taaagtttcg aataccttcg tgaggaacaa tttgctctgg actaacaaaa
     2221 tctccagcgt aagaggtaat ttcatctgta acgatacttc cgaatccttc acctgatgtt
     2281 tctaatctat tatctacgat aacgtttggc tgataatgac gaaccatgtc tattaatttt
     2341 gaagctcccc atttttcacc caccatgtct tcataagaat aatcaaacca taatatgtca
     2401 atctgaccat aaccagttac aatttcttta acttgattgt gcaaatactc caaataactg
     2461 tcaaagttaa ttttctcatc tcgataggct tcatttcctc tcattggatg atttagatca
     2521 gcatatttag ggaaatcagg atggtgccag tcaatcaaac taaaatataa gcctactttt
     2581 aaaccttcag ctcgaacagc atctacaaat tctttaacta aatctctacc tgcaggtgta
     2641 tttgttgctt tataatctgt aaattttgaa tcaaacaaac aaaacccatc atgatgttta
     2701 gccgttaaaa ctatatactt catccctgca gcttttgctt gtttagccca ttctctaggg
     2761 ttatattcct taggatcaaa agctcgaaaa tatggttcat aatcttcaat agataatttc
     2821 tgatgactac gaatccattc cccttttcct ggaatagaat ataaccccca atgaatgaac
     2881 atcccaaatc ttgcttctct aaaccactga gttctactat tgatagtttc aagtgtatgt
     2941 ttcatccttt tactgctcct cctcctaaag cttcaatgaa gtatttactt gtaaatgcga
     3001 atagaatgat tggtggtaat acgattacaa caatagctga aaatagacca ttataattag
     3061 tattaaaact tgtagtaaat tgacctagta atgctggtac tgttaattca tttttatcaa
     3121 tcaccaacat agatgcccaa taatattcat tccagttatt aataaaggca agtactgctg
     3181 ctgttaaaat tcctggtcta gcaattggca acatcacttt ccaaaaagtt tgagcatatg
     3241 tcgcaccatc aatctctgct gcctcctcca attcttttgg aataatttca aaatagttct
     3301 ttataataaa gaaactcata gcaattccac tacaagagta gaccagaatt agagcaactg
     3361 gggtattata aatccccaac tcatttacta atctataaac tggaaaagtc atagctgttg
     3421 ctggaataaa tagagtagaa tacagcatag aagtcaccaa cttcttccct ctaaattcca
     3481 tcctagcaat aacatatgaa gacatggaga tcataacaat gctaataata actgatatag
     3541 acactacttt aaagctattc caaaagtaag tgaaaacatg aagttttgta aaaacattta
     3601 tataaccttc aagagtaaag tcagtcggta aactaatacc tggatttttc ataggatctg
     3661 cttttaatga tgataaaaaa atccacgcaa tagggaaaat agaaattaaa atggtaagga
     3721 g
//
LOCUS       DAVLSL010000153         3664 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLSL010000153 DAVLSL010000000
VERSION     DAVLSL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.155"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            138..1367
                     /gene="arcA"
                     /locus_tag="VXN30_001855"
     CDS             138..1367
                     /gene="arcA"
                     /locus_tag="VXN30_001855"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1358429.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1425..2441
                     /gene="argF"
                     /locus_tag="VXN30_001856"
     CDS             1425..2441
                     /gene="argF"
                     /locus_tag="VXN30_001856"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1358430.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNKNLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2606..3553
                     /gene="arcC"
                     /locus_tag="VXN30_001857"
     CDS             2606..3553
                     /gene="arcC"
                     /locus_tag="VXN30_001857"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1358431.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         1163 a          714 c          762 g         1025 t
ORIGIN      
        1 aattgtgcta aatatcataa tttatattat tttccgagaa attatagcca ttgacattac
       61 ctaggaattg cgttataaca tacatgtaag cggtacccaa aatggtgcca agtcaaaatt
      121 tttaaggagg aaaatacatg tcttctcatc caattcaggt cttctcagaa attgggaaac
      181 tgaaaaaagt tatgttgcac cgtccaggca aggagttaga aaacttgttg ccggactatc
      241 ttgaaaggct tctttttgat gatattcctt tcttggaaga tgctcaaaaa gaacatgatg
      301 catttgccca agctcttcgc gatgaaggaa ttgaggttct ctacctagaa caactcgctg
      361 ctgaatcatt gacctctcca gaaatccgcg atcaatttat cgaggaatac ttagacgaag
      421 ccaacatccg tgatcgtcaa actaaggttg ctattcgtga attgcttcac ggcatcaagg
      481 acaaccaaga attggttgaa aaaacaatgg ctgggattca aaaagttgaa ttgccagaaa
      541 ttcctgacga agctaaagat ctaactgact tagttgaatc agagtatcca tttgcaattg
      601 acccgatgcc aaacctctat ttcactcgcg acccatttgc aacaattgga aacgccgtat
      661 cgcttaacca catgtttgca gacactcgta accgtgaaac actctacggt aagtatatct
      721 tcaaatacca cccaatctat ggcggaaaag tggatttggt ctacaaccgt gaagaagata
      781 cgcgtatcga aggtggagac gagctagttc tttctaaaga cgtccttgca gtaggtatct
      841 ctcaacgtac agacgcagct tctatcgaaa aacttttggt caacatcttc aagaaaaatg
      901 ttggcttcaa gaaagttttg gcctttgaat ttgctaacaa ccgtaaattc atgcacttgg
      961 atactgtctt cactatggta gactatgaca agttcactat tcacccagaa atcgaaggcg
     1021 accttcacgt ttactcagtt acttacgaaa acgaaaaact taaaatcgtt gaagagaaag
     1081 gtgacttagc tgaacttctt gctcaaaacc ttggtgtaga aaaagttcat ttgattcgtt
     1141 gcggtggtgg caatatcgta gcagctgcgc gtgaacaatg gaacgacggt tctaacactt
     1201 tgaccatcgc acctggtgtg gtagttgttt atgaccgcaa taccgtgacc aataagattt
     1261 tggaagaata cgggcttcgc ttgattaaga ttcgcggaag tgaattggtt cggggccgtg
     1321 gtggacctcg ttgtatgtct atgccatttg aacgtgaaga agtgtaatcg ctgttcggca
     1381 ttcgtcaata gaaaatgtaa aaaatagaaa gaggaaataa taaaatgaca aattcagtat
     1441 tccaaggacg cagcttctta gcagaaaaag actttacccg tgcagagtta gaatacctta
     1501 ttggtctttc agctcacttg aaagatttga aaaaacgcaa tattcaacac cactaccttg
     1561 ctggcaagaa tatcgctctc ctatttgaaa aaacatctac tcgtactcgt gcagccttta
     1621 caactgcggc tatcgacctt ggtgctcacc cagaatacct cggagcaaat gatattcagt
     1681 tgggtaaaaa agaatctact gaagatactg ctaaagtatt gggacgtatg tttgacggga
     1741 ttgaattccg cggattcagc caacgtatgg ttgaagaatt ggcagaattc tcaggcgttc
     1801 cagtatggaa cggtctaact gacgaatggc acccaactca aatgctcgct gactacttga
     1861 ctgttcaaga aaacttcggt cgcttggaag gcttgacatt ggtatactgt ggtgatggac
     1921 gtaacaacgt tgccaacagc ttgctcgtaa caggtgctat ccttggtgtc aatgttcaca
     1981 tcttctcacc aaaagaactc ttcccagaaa aagaaatcgt tgaattggca gaaggatttg
     2041 ctaaagaaag tggcgcacat gttctcatca ctgaagatgc tgatgaagca gttaaagatg
     2101 cagacgttct ttacacagac gtttgggtat caatgggtga agaagacaaa ttcgcagaac
     2161 gtgtagctct tcttaaacct taccaagtca atatggactt agttaaaaaa gcaggcaata
     2221 aaaacttgat cttcctacac tgcttgccag cattccacga tactcacact gtttatggta
     2281 aagacgttgc tgaaaaattt ggtgtagaag aaatggaagt aacagacgaa gtcttccgca
     2341 gcaagtacgc tcgccacttc gatcaagcag aaaaccgtat gcacactatc aaagctgtta
     2401 tggctgctac acttggtaac ctttatattc ctaaagtata attttagata ataaaccgtc
     2461 taccaacagc tatgagggct gcgactaata gctttagtcc ggtcctcttt tatgtaatgg
     2521 taatctatta tttcttataa aatatgtgaa aaatcattaa attgaaatct aaacgcattc
     2581 tattgagtgt gataaaggag aatttatggc aaatcgtaaa attgtagtag ctttgggagg
     2641 aaatgcgatt ctttcttctg acccatcagc aaaggctcaa caagaagctt tagttgaaac
     2701 agctaagcat cttgtaaaat tgattaaaaa tggagatgat ctgattatca ctcacggtaa
     2761 tggacctcaa gttgggaatc tcttgctcca acatttggca tcagactctg aaaagaaccc
     2821 tgccttccca ctcgactcac ttgtcgctat gacagaaggt agcatcggtt tctggttgaa
     2881 aaatgctttg caaaatgctc tcttggatga aggcatcgaa aaaaatgttg cctctgttgt
     2941 aacgcaagtt gtcgtagata aaaatgatcc agcttttgtt aacttgagta aaccaatcgg
     3001 tcctttctat tcagaagaag aagcaaaagc agaagccgaa aagagcggag cgactttcaa
     3061 ggaagatgct ggccgtggct ggcgtaaggt cgttgcctca ccaaaacctg ttgacatcaa
     3121 agaaattgaa accatccgta ctcttttaaa taatggtcaa gtcgtcgtag ctgcaggtgg
     3181 tggcggtatt cccgtcgtca aagaaaacaa tggacatttg actggtgtcg aagcggttat
     3241 tgataaagac ttcgcttccc aacgtttggc agaattggtt gatgcagacc tcttcatcgt
     3301 tttgacaggt gtagattatg tatttgttaa ctacaacaag ccaaaccagg aaaaattgga
     3361 acatgtgaat gttgcccagc tggaagaata tatcaaacaa gatcagtttg caccaggtag
     3421 catgcttcca aaagtagaag cagctatcgc ttttgtcaat ggtcgtccag aaggaaaagc
     3481 agttattact tcccttgaaa atctaggcgc cttgattgaa tctgaaagcg gaacaattat
     3541 tgaaaaagga taagttgttt tactaataag atgtattcta tttctagtat ctttatatca
     3601 aattagaaat tattcttgaa aacatgtaca atatttcaaa agatactagt tttagacttt
     3661 aata
//
LOCUS       DAVLSL010000154         3626 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLSL010000154 DAVLSL010000000
VERSION     DAVLSL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.156"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(115..3027)
                     /gene="nanA"
                     /locus_tag="VXN30_001858"
     CDS             complement(115..3027)
                     /gene="nanA"
                     /locus_tag="VXN30_001858"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1358432.1"
                     /translation="MNRSIQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDASAPRFYNLFSVSS
                     DTKENEYFTMSVLDNTALIEGRGANGEQFYDKYTDAPLKVRPGQWNSVTFTVEQPTAE
                     LPHGRVRLYVNGVLSRTSLKSGNFIKDMPDVNQAQLGATKRGNKTVWGSNLQVRNLTV
                     YDRALSPEEVQTRSQLFERGDLEKKLPEGAKISEKEDVFEGGMNNKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKERTIREEF"
     gene            complement(3315..3542)
                     /locus_tag="VXN30_001859"
     CDS             complement(3315..3542)
                     /locus_tag="VXN30_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358433.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
BASE COUNT          945 a          836 c          621 g         1224 t
ORIGIN      
        1 actctgaaag tagtttcaga cctcttatga tttcgtatca gcctgaatgt catcaaaaaa
       61 agatagcagg cttgaaaacc tgctatctcc ttctattttt acaaaatcaa atgtttagaa
      121 ttcttctctt attgttctct cttttttccc tagcgtaaac agaccaagga agaaagctgt
      181 tagtcctagt gaagctagga ggtcactctc cttgttccct gtttcaggaa gtgcctgctg
      241 agcaagtgga gctttgtaag tataatcttc ttttgtagta agagttacaa gcgaatcaga
      301 tcccttatac tctgcgattt catgaacagc tggctctgta ccattaactc cccctgtaaa
      361 ttctggaact tcatgcaccg ctgctttgct accatttact ccgccaggaa ctctggcacc
      421 tgctagattt acaggaagat tatgcatact ttcgatgctt cctttagcta taccaataat
      481 ttcctgtccg atatcttcct tatctactgc aaacaacaag gttttgctat catactgggt
      541 taggaaagtc gctgttttac catttgccaa ttgaagagtt ggagccttgt tgaccaatac
      601 ttctgagtcg aactccaagc caataactcc tttgcccatc tctcctttgc ccatctctct
      661 agttcgcttc actttcgctt cggtaggaga aatcagattt ttgctcaaaa attcccaatt
      721 aaattttcta aatgataggg tataggcatt ttgtcctttt tcagtatgtt catacaagat
      781 gccatactcc ccatttccta attcttggag cgaattatag gcaaactctc ctttttgaat
      841 tggattgtgt ttgagccaag tcaactcacc attttcttcg acacgtgcca agtggaccat
      901 cccattttca cgtttcggtc cacctgcatt actgaggatg atgtattctt ttccttcgtg
      961 catcgtatgg atagcagaca tttgaacata gacatcttta acctgtggat aacgtttgat
     1021 atccttctcc caagtcactc ctccgtcttt acttgtagca acctgaagat ctccagtcaa
     1081 accacgcata aagagtttaa catctccatt gtttagttgt accaccgttg attctgtatt
     1141 ttgcgcacgt ttattgttca tcgtagaaga gtggatcttt tgaccgtcta cctgacggtt
     1201 atcgttgacc gcttctccag catgccaagt ttttccatga tcatctgaat agatgacacg
     1261 agaagattgc gagccattta agtgagatac attattagtc gtataaaccg gtatcaaaat
     1321 ccgtcccttg tgaggcccat tccgaagtac aattcctgtt ccaggaccta cacccaagaa
     1381 tttcatccaa tcggctttga ccatcggagt aatatcttga ggagctgacc atgtcttccc
     1441 gtcgtcatca ctgtaggaca tccatagata gctatccttg gcaattctaa atggggaagt
     1501 tttgtttgtt gtgaagtaga tattacctag taattggtca cccttgtata gatcaccctt
     1561 gtcgctatag gctggtttaa caggatctac aacaacgcga tagtctgtcg ccttgccatc
     1621 tggtgtgtag acagtaccat tttcacgaat ggtataagct cccttttctc cttcacgata
     1681 gaggatttga taggtttttc cgtcaatttc tttgtaggct tcttcttttt gcgaagacat
     1741 tccaaagatt cccttccctt ctgggaacat gtcatagatg gaaaagattc gtttggtttc
     1801 aggatcttga accaacacca tatcgatatt cactggtgaa ccgatcgatg gatcagaagc
     1861 ttttggattg tcacgtaagt tggtaatggt tactcggtct ccccaagttt taccattgtc
     1921 ttcactacgt ctgataacca taccgatatc accccagtca ctagaatgaa gacggcgttc
     1981 atctgcacct gcaatcagag ttcctttatc tgtcttgaga agagctggaa tacgataact
     2041 cttaattcca tctttatttg gtttgttgtt cattccccct tcaaagacgt cttctttctc
     2101 tgagattttc gcaccttctg gaagtttctt ctccaaatca cctctttcaa atagttggct
     2161 tctcgtctga acttcttctg ggcttaaggc acgatcatag acggttaggt ttcgaacttg
     2221 tagattgctt ccccaaactg tcttattgcc ccgtttggtt gctccaagtt gagcttgatt
     2281 aacatctggc atatctttga taaagttacc agatttgaga cttgttcgag ataagacacc
     2341 gttcacatag agacgaactc gtccatgagg taactccgct gtcggttgtt cgacagtaaa
     2401 ggtcacggaa ttccattgtc ctgggcgaac tttcaaagga gcatctgtat acttatcata
     2461 gaactgctct ccgttagcgc cacgcccttc aataagagct gtattatcaa ggaccgacat
     2521 agtaaagtac tcattttcct tggtgtcact ggatacagag aagagattgt aaaagcgtgg
     2581 agctgatgca tctggcttaa actccatgtg aactgttgcg ttttcaagtt tctttagttt
     2641 atctagttca cttgataaat caactctctg accatttgaa gcattggttt caacatcttc
     2701 tttttctatc actgtttcga cattttccaa atctcgtgca tagtaatctc ttggaatttt
     2761 ttcttcttgc ttatctttcc tttcttgttc ttgcttattg ccagaaagtt cagtctctga
     2821 agaaggctgg ctcttttctg tatcagttag ggttgagctc ccccccgaaa gttgagtttc
     2881 atttgccaga ggttgctcac ttgccccttc ttgagctaaa acaggagacg ttccaaatac
     2941 cactgctcct acaatcatag aaaccgctcc taccgatagt ttcctaatgc tataacgaca
     3001 cttacgttct tgaatactcc gattcatact gatcctctct atatctatat cccgatttct
     3061 gaaataagac atttactcca gaaaatgcac ttagtttgtc ttttattaac tttcttttac
     3121 tacagttcat ttaaatgaca aaaatgaact aaacaaacta tctcaaattg gtgccaattt
     3181 tcccctctcc tttctccaat cagactcctg ccaatctagt ttatctacca gggcaccgct
     3241 gcaattaaac taaaatatct ctttataagc aaatgatgga catcagctta tgctatttat
     3301 accatacttc ctgctcaatc cttatcgaac actttttcaa atagcttttc ataagcataa
     3361 atacttgtag aaataatggg aaatataatc aagctcacaa tcagaatctt atccagctta
     3421 aaggggacaa tcccaaataa gtgaagaaat aaatcaatca gaaaactgtc gaaaaaaact
     3481 gtcaaaagta gttcaacttg tctttttctc tgcttttttg tcttgaatgc cttaaatatc
     3541 atattattcc ccttttctaa gcttcaatcc cttctctcta tctgttattt cgcatagcta
     3601 ctactatttt tacagcaatc tatctt
//
LOCUS       DAVLSL010000155         3621 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLSL010000155 DAVLSL010000000
VERSION     DAVLSL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.157"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            126..566
                     /gene="adcR"
                     /locus_tag="VXN30_001860"
     CDS             126..566
                     /gene="adcR"
                     /locus_tag="VXN30_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1358434.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            566..1270
                     /locus_tag="VXN30_001861"
     CDS             566..1270
                     /locus_tag="VXN30_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1358435.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            1263..2069
                     /locus_tag="VXN30_001862"
     CDS             1263..2069
                     /locus_tag="VXN30_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1358436.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            2079..3584
                     /gene="adcA"
                     /locus_tag="VXN30_001863"
     CDS             2079..3584
                     /gene="adcA"
                     /locus_tag="VXN30_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1358437.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGKEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQIKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT         1056 a          731 c          817 g         1017 t
ORIGIN      
        1 aaactagttt tcacgttttt tcttgactct ttttttgctt gtgatataat taactggtaa
       61 acaaaatgtc aaagaatagt atttttctct taaaaaagag aagtctaaaa ggaaaggagt
      121 cagatatgag acagctagca aaggatatca atgctttttt gaatgaggtg attttgcagg
      181 cggaaaatca gcatgaaatc ctaataggtc attgcactag cgaggtggcc ctgaccaata
      241 ctcaggagca tatccttatg ctcttgtcag aggaatcttt aacaaattca gaattggccc
      301 gtcgtctcaa tgtcagtcag gcggcagtta ccaaggccat taagtctttg gtcaaggaag
      361 ggatgttgga aacatctaaa gattctaaag atgcgcgtgt gattttttat cagttgactg
      421 acttggctcg tccaatcgct gaggagcacc accatcacca tgagcataca cttttaacct
      481 atgaacaagt ggcgactcag tttactccaa atgaacaaaa agtgattcag cggtttttga
      541 ctgctttagt aggagaaatc aaataatgag atatattacg gtagaggatt tgtccttcta
      601 ttatgataag gagcctgttc ttgaacatat caattatagt gttgatagtg gggaatttgt
      661 taccttgact ggggaaaatg gagcggctaa gacgacgctc atcaaggcca gtcttggaat
      721 tctgcaacca cgcattggaa aggtggctat ttcaaagaca aatacgcaag gtaagaaatt
      781 gagaatagcc tatcttcctc aacaaattgc cagttttaat gctggttttc caagtacggt
      841 ctatgaattt gtcaagtcgg gtcgttatcc gagaaaaggc tggttccgtc gtttgaatgc
      901 tcatgatgag gagcatatca aggccagtct ggactcagtt ggcatgtggg aacatcgaga
      961 caaacgcttg ggttctctat ctgggggaca aaagcagcga gcggtgattg cgcgtatgtt
     1021 tgcttctgac cctgatgtgt ttatcctaga cgagccgaca acggggatgg atgcaggaag
     1081 taaaaatgaa ttttacgaac tcatgcatca cagtgcccat catcatggca aggctgtttt
     1141 gatgattacc catgaccctg aagaagttaa ggattatgcg gatcgcaata ttcatctagt
     1201 ccgtaaccaa gactcgccat ggcgttgttt caatgttcat gagaatggcc aggaggtggg
     1261 ccatgcttag tttattatct tacgacttta tacaacgcgc ctttttggcg gttattgcta
     1321 tgagtctttt ctcaccggta ttgggaacct tcctcatctt gcgtcgtcag agtttgatga
     1381 gtgataccct tagccacgtc tcactttcag gtgtagcctt tggtctggtt ttggggattt
     1441 ctccaactgt ttctactatt gccattgtct tgattgcggc ggtctttctg gagtatctcc
     1501 gtacggttta caagagcttt atggaaatcg ggacagctat cctcatgtca acaggtctgg
     1561 ctgtttctct gattgtcatg agcaagggta aaagctcgag ttcaatgagt ttggaccaat
     1621 atctctttgg ttcgatcgtg actatcagtg aagaacaggt catttccctc tttgtcattg
     1681 cggcggttgt tttgattttg acctttctct ttcttcgtcc tatgtatatc ttaacttttg
     1741 acgaagatac ggcctttgtg gatggcttgc cagttcgtac catgtccatt ctttttaaca
     1801 tggtgacagg ggtggctatt gcccttatga ttcctgcagc aggagctctt ctggtatcga
     1861 ccattatggt cttgccagct agtattgccc tgcgtctggg gaaaaacttt aaatcggtta
     1921 tgctgcttgc cagtgcgatt ggctttttgg gaatggtagc aggactttac atttcctact
     1981 atgcagaaac acctgcaagt gcaagtatta ccattatttt tgtaactgtc tttatactaa
     2041 tcagtttagt aagacgtttt atcaaatagg agacgaacgt gaaaaaaatt agcttattac
     2101 tagccagtct atgtgccttg tttttagtgg cttgttccaa tcaaaaacag gcagatggta
     2161 aactcaatat cgtgacaacc ttttaccctg tctatgaatt taccaagcaa gtcgcaggag
     2221 atacggctaa tgtagaactc ctaatcggtg ctgggacaga acctcatgaa tacgaaccat
     2281 ctgccaaggc agttgccaaa atccaagatg cagatacctt cgtttatgaa aatgaaaaca
     2341 tggaaacatg ggtacctaaa ttgctagata ccttggataa gaaaaaagtg aaaaccatca
     2401 aggcgacagg cgatatgttg ctcttgccag gtggcaagga agaagaggga gaccatgacc
     2461 atggagaaga aggtcatcac catgagtttg acccccatgt ttggttatca ccagttcgtg
     2521 ccattaaact agtagagcac atccgcgaca gcttgtcagc agattatcct gataaaaaag
     2581 agacctttga gaagaatgca gctgcctata tcgaaaaatt gcaagccttg gataaggctt
     2641 acgcagaagg cttgtctcaa gcaaaacaaa agagctttgt gactcaacac gcagccttta
     2701 actatcttgc cttggactat ggactcaaac aagtcgcaat ctcaggactt tctccagatg
     2761 cagagccatc agctgctcgc ttggcagaat tgacagagta tgtcaagaaa aataaaatcg
     2821 cctatatcta ttttgaagaa aatgcctcac aagcccttgc taacacactt tcaaaagaag
     2881 caggtgtcaa aactgatgtc ctcaatcctt tagaaagtct gacagaagag gacaccaagg
     2941 ctggagaaaa ctacatttcc gtgatggaga aaaacctcaa ggctttgaaa caaacaacag
     3001 accaagaagg cccagcaatc gaacctgaaa aggcagagga taccaagaca gtccaaaatg
     3061 gttacttcga ggatgcagct gtcaaggacc gcaccttgag tgactatgca ggtaactggc
     3121 aatcagttta tcctttcctt gaagacggca cgtttgacca agtctttgac tacaaggcta
     3181 agttgactgg taagatgacc caggctgagt acaaggctta ctatacaaaa ggctatcaga
     3241 cagatgtgac taagattaac attactgata atactatgga atttgttcaa ggtggacaaa
     3301 tcaagaaata cacttacaag tatgtcggta agaaaatttt gacttacaag aaaggcaatc
     3361 gtggcgtgcg tttcctcttt gaagccacag atgctgacgc tggacaattc aagtatgttc
     3421 agtttagtga ccacaatatc gccccagtta aggcagaaca tttccatatc ttctttggag
     3481 gcacaagcca agaagccctc tttgaagaaa tggacaactg gccaacctac tacccagata
     3541 acctatctgg ccaagaaatc gcccaagaaa tgttggcgca ttgatgagaa atcgactagt
     3601 tcataagagc tagtcggttt t
//
LOCUS       DAVLSL010000156         3602 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLSL010000156 DAVLSL010000000
VERSION     DAVLSL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.158"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(120..1256)
                     /gene="lctO"
                     /locus_tag="VXN30_001864"
     CDS             complement(120..1256)
                     /gene="lctO"
                     /locus_tag="VXN30_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1358438.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDKRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1882..3372)
                     /gene="lysS"
                     /locus_tag="VXN30_001865"
     CDS             complement(1882..3372)
                     /gene="lysS"
                     /locus_tag="VXN30_001865"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1358439.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3500..>3602)
                     /locus_tag="VXN30_001866"
     CDS             complement(3500..>3602)
                     /locus_tag="VXN30_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1358440.1"
                     /translation="NTYDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         1025 a          774 c          709 g         1094 t
ORIGIN      
        1 ttaactgtcc tgattgattt gtcctgtttt tattccattt tactatggtt ttcgatagcg
       61 atttattctt ccaatacacg aagaaaaacc tccacattca gtggaggcaa tctcttttat
      121 caatacaatt ttaagtcacg agggtcaact gggaaagttg ggttgtatgg gttgtgacgg
      181 agtttgaagt gtttaacatc ttcaatagtc tgagttccag acaactgcat aactgtcttc
      241 aattccgcat tcaagtgttc aaagacttga cgcacaccaa cactaccgcc gagagccaag
      301 ccatagataa ccgggcgacc aatagctacc aagtctgctc ctgaagccaa ggctttaaag
      361 acatgttgac cacgacgaac accagagtca aagacaattg gcacacgttt atcaactgct
      421 tctgccactt cttgaagcga gtcaaaggca gctggtccac cgtcgatttg acgaccaccg
      481 tggttggtta cccagatacc agaagctcct gcagcaagcg aacgttcaac gtcctcacgg
      541 cattgtggtc ccttgacata cacaggaagt cctgagtatt cagcgataaa ttctacatca
      601 cgtggagaca agcgttgttt agctgatttg taaacaaagt ccattgattt accagcacct
      661 tctggcaggt attcttcaac aatcggcatg ccaactggga agacaaaacc attacgctta
      721 tccacttcac gattcccccc tacagtagca tctgccgtca agacaatcgc tttataacct
      781 tcagccttca cacgatccat gatgtggcgg ttgataccgt catccttact aaagtaaaat
      841 tggaaccaat ggggtgtccc ttgaagggct tcagaaatct ctggaaggtc aacagtagag
      901 taagaactgg ttgtataaag agaaccaaac tcatgcacac cacgcgcagt cgccacttcc
      961 ccctgttcat tcgctaattt atgggccgca acaggcgcca taatgattgg tgaagatagt
     1021 ttttcacctg caaattcaat ctctgtactt ggattttcaa catcgcaaag tgtatgagga
     1081 acgatgagtt tgtggttaaa ggcgcggata ttctctctta aagtgaaagt atcttccgcc
     1141 ccactagcga tatagccaaa tgctgcttta ggaataactt gttgcgccat tggctccaaa
     1201 tcataggtat tgatgaagtc tacatgacct tctgcattgc ttgttttgta tgacataaaa
     1261 tgccctcctt gattaagtaa gcgtttgctt tttactacac aactataata actctttttt
     1321 caatcatttt caagagtttt gcttaaaaac catcttattt tttcatacaa tatactagcc
     1381 ctactttcct taaactctaa accttagtct atattaagtg aatcgttgag attttttgct
     1441 gccaggcatt tgaactactt ttttatttgc ggattctatc tactaatact ataaaaaaaa
     1501 caagtgaata agacaccaga acatcctcta ataggaccta tggacttata tcagtcccat
     1561 atatactgtt taagcaagat actatcctag ttgacgctaa cttcctatcc cacttattaa
     1621 ttttttattt atagtgaatc tgaacaaatc aattggggaa tccaagtcaa tttctagtac
     1681 tttttgaaca gtttcagcgt actatcgtaa aatgaaataa aacatgcaca aattgcttag
     1741 ggaatttaaa ccaatttcta acaatgtctt agaaatcaaa gtgtactatt tcaacttcaa
     1801 tatactacac ttcattttct ccttaaattc agtctctttt tgaatcaaaa aatcccctgg
     1861 taagagccag aggataagaa tttatttcat tgttgggaag agcaatacat cacggatagt
     1921 tgttgtatca gtgaggagca tgcagagacg gtcgataccg attcccaaac cacctgttgg
     1981 tggcataccg tattcaagag cttcaatgta gtcatagtcg attcctgtcg cttcatcatc
     2041 accaagttct ttggctttag cttgggcttc aaaacggcta agttggtcga ttgggtcgtt
     2101 caactcagta aaggcattac cgtactcctt agtcatgata aagagctcga aacggtcagt
     2161 aaagcgttgg tcttcaggat ttttcttagc gagtggagat acagctactg gatgtccata
     2221 gacaaaggtt ggttggatta aagtttcttc aacaaactct tcaaagaagg cattgatgat
     2281 gtgaccaacc tcagtgtagt gtttctcaac tggaactttc ttctcagcag cgatagcttt
     2341 agcttcttcc aaagtcatgt cttgccagaa atcgacacca gtaatttctc tgatagcatc
     2401 caccatatga acacgcttaa atggttcgtt aatcttgatt tcagtacctt ggtagttgac
     2461 tgggccatca cctttgactg atttagcagc gtgttggata atgccttcag tcaagtccat
     2521 gatgtcttgg aagtctgcat aagcttggta aacttcgata gaagtgaact cagggttatg
     2581 agtagcgtcc attccttcgt tacggaagat acggccaatt tcatagacac gttccatacc
     2641 acccacgata aggcgtttta agtgaagctc agtcgcgata cgaagcacca tgtcaatgtt
     2701 ttgggcattg tggtgggtga taaatggacg ggcagcagca ccaccggctt cattatgaag
     2761 aacaggtgtt tccacttcaa ggaatccttt ttggtcaagg taacgacgga tttcagagat
     2821 gatttttgaa cgagtgacaa agcgttcaaa gctttcacga ttagaaatca agtcaaggta
     2881 acgtttacgg taaattgttt caacgtctgt caaaccatgg aatttctcag gaagaggacg
     2941 aagagcctta gacaagtgtg tgatgtgggt tgccttgata gagagttctc ccatatccgt
     3001 acgcatcact tcaccttcga caccaaggaa gtcaccaagg tctgcttttt tgaagatttc
     3061 gtagttttct tcaccgacag catccttacg aacgtagatc tgaatctggc cttcgcggtc
     3121 ttgaaggtgg gcaaaaccaa cttttccttt accacgtttg gttaccaagc gtcctgcgat
     3181 agtagctgtt tcgtttttat cgtgtaattg ttctttatcg aggttggtat atttatcttt
     3241 taattcttgt gaatttgcag tacgttcaaa acgttttccg aaaggatcga ttccttgttc
     3301 gcggagcgca gccatttttt cacggcgaac gatctgctgg tcatttagtt cttccatatg
     3361 ttctgtagac atgggatcct cctagtttta ctcaaaattg aatacgatga gtcatttttt
     3421 gcgatactcc cattttatca taaataagca agaaattcac ggataatctt taaaaactaa
     3481 aaagaacctg acgctaaaat cactggaaag aagtgaaagt atctcccgcc ccactagcga
     3541 tatagccaaa tgctgcttta ggaataactt gttgcgccat tggctccaaa tcataggtat
     3601 tg
//
LOCUS       DAVLSL010000157         3599 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLSL010000157 DAVLSL010000000
VERSION     DAVLSL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.159"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            133..1482
                     /locus_tag="VXN30_001867"
     CDS             133..1482
                     /locus_tag="VXN30_001867"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1358441.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            1606..3066
                     /locus_tag="VXN30_001868"
     CDS             1606..3066
                     /locus_tag="VXN30_001868"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1358442.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            3080..3511
                     /locus_tag="VXN30_001869"
     CDS             3080..3511
                     /locus_tag="VXN30_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1358443.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT         1212 a          682 c          753 g          952 t
ORIGIN      
        1 caaaattttt agaatgtggc attttacgca aacgtttgaa ttctgataaa aattggagaa
       61 attcgacaaa aaaacttgaa aaaaacgaag gtaagcgtta tgatagaaaa gaagaaatat
      121 tggaggaaga acatgtcaca tattaaattt gattattcaa aagttttaga caaatttgtt
      181 gcaccacatg aagtggaata catgcaatca caagtaacag cagcagatga attgatccgt
      241 aaaggaactg gtgctggtag cgacttcttg ggatggttgg accttcctga aaaatacgac
      301 cgcgaagaat ttgaccgcat cttgaaagct gctgagcaaa tcaagtcaga cagcgatgtt
      361 ttggttgtga tcggtattgg tggatcttac cttggtgcaa aagcagcaat cgacttcttg
      421 aatcaccatt ttgctaactt gcaaacaaaa gaagaacgca aagctccaca aatcctttac
      481 gcaggaaact caatctcatc tacttacctt gctgacttgg tagagtatgt tgcagacaaa
      541 gacttctcag taaacgtaat ttctaaatca ggtacaacaa ctgaaccagc gattgctttc
      601 cgtgtcttta aagaactctt ggttaagaaa tacggtcaag aagaagctaa caaacgtatc
      661 tatgcaacaa ctgaccgcca aaagggtgct gttaaggttg aagcagacgc taacggttgg
      721 ggaacatttg ttgttccaga tgatatcggt ggacgcttct cagtattgac agccgttggt
      781 ttgcttccaa tcgcagcatc aggagctgac ataaaagctc ttatggaagg tgcgaatgca
      841 gctcgcaaag actacacttc agacaaaatc tctgaaaacg aagcttacca atacgcagtc
      901 gttcgtaaca tcctttatcg taaaggctat gcaactgaga tcttggtaaa ctatgagcca
      961 tcacttcaat acttctcaga atggtggaaa caattggctg gtgaatcaga aggaaaagac
     1021 caaaaaggta tctacccaac ttcagccaac ttctcaactg acttgcactc acttggtcaa
     1081 tttatccaag aaggaactcg tatcatgttt gaaacagttg tccgtgttga caaacctcgt
     1141 aaaaacgtgc ttattcctac tttggaagaa gaccttgacg gacttggtta ccttcaagga
     1201 aaagacgttg actttgtaaa caaaaaagca actgacggtg ttcttcttgc ccacacagat
     1261 ggtgatgtac caaacatgta tgtgactctt ccagagcaag acgctttcac tcttggttac
     1321 actatctact tcttcgaatt ggcaattgcc ctttcaggtt acttgaatgc tatcaaccca
     1381 tttgaccaac caggtgttga agcttataaa cgtaacatgt ttgcccttct tggaaaacca
     1441 ggatttgaag aattgagcaa agaacttaac gcacgtctat aatagaagaa aagagtggtt
     1501 tgcccactct ttttactctc tttatccata gaaattggac tcagccaaga cttgtgatat
     1561 aatatagaaa gcaaaaaggc agacgcctag ataataggag aaactatgtc aaaagatatc
     1621 cgcgtacgtt acgcaccaag tccaacagga ctactacaca tcggaaatgc ccgtacagca
     1681 ttgtttaact acttgtatgc gcgccatcat ggtggaacat ttctcatccg tatcgaagat
     1741 actgaccgta aacgccatgt cgaggatggt gaacgttcac aacttgaaaa ccttcgctgg
     1801 ttaggcatgg attgggatga aagtccagaa tcacatgaga attatcgcca gtctgagcgt
     1861 ttggacttgt atcaaaaata cattgaccaa ctattagctg aaggaaaagc ctataaatct
     1921 tacgttacag aagaagagtt ggcagctgaa cgtgaacgtc aagaagtagc tggcgaaaca
     1981 ccacgctaca tcaatgaata ccttggtatg agtgaagaag aaaaagcggc ttatatcgca
     2041 gaacgtgaag cagctggtat cataccaaca gttcgtttgg ctgtcaatga gtcaggtatc
     2101 tacaagtggc atgatatggt caaaggtgat atcgaatttg aaggtggcaa tatcggcggt
     2161 gactgggtta tccaaaagaa agacggttac ccaacttaca actttgccgt tgttatcgat
     2221 gaccacgata tgcaaatctc tcatgttatc cgtggagatg accatattgc taatacacca
     2281 aaacagctta tggtctatga agctcttggt tgggaagctc cagagtttgg tcacatgacc
     2341 ttgattatca actctgagac tggtaagaag ttatctaaac gtgatactaa tacacttcaa
     2401 tttatcgaag attaccgaaa gaaaggttac cttccagaag cagtctttaa ctttattgct
     2461 cttcttggtt ggaacccagg tggtgaagat gagattttct ctcgtgaaga actcattaaa
     2521 ctttttgatg aaaaccgcct cagcaagtca ccagcagcct ttgatcagaa aaaattagac
     2581 tggatgagca atgattatat caagaatgca gaccttgaaa ccatctttga aatggcaaaa
     2641 ccattcttag aggaagcagg ccgtttgact gacaaggctg aaaaattagt tgagctctat
     2701 aaaccacaaa tgaaatcagt agatgagatt atcccattga cagatctttt cttctcagat
     2761 ttcccagaat tgacagaagc agagcgcgaa gtcatgacgg gtgaaacagt tccaacagtt
     2821 cttgaagcat tcaaagcaaa acttgaagcg atgacagatg atgaatttgt gacagaaaat
     2881 atcttcccac aaattaaagc agtccaaaaa gaaacaggta ttaaagggaa aaatcttttc
     2941 atgcctattc gtatcgcagt ttcaggcgaa atgcatgggc cagaattacc agatacaatt
     3001 ttcttgcttg gacgtgaaaa atcaattcag catatcgaaa acattctaaa agaaatctct
     3061 aaataaagag gatacaataa tggacatctg ggaaaagatg tacgaagaag cacagaaact
     3121 atacaatcca catgaagtat cagactttgt ttatgccaat catgttgtag ctgcagtaga
     3181 agcaggagat ggacaaatat ttacaggatt ctgtatggag ggaacctgtg gtgttttcca
     3241 tctctgcgca gaacgggcga ccctcttcaa tatgtaccaa ttttcaggac aaactaaggt
     3301 taagaaagta ttagcctttc gagacaaacc accttatggt ggaagttcag ccatgccttg
     3361 cggtgcttgt agagaattcc ttttagagct taatgctgaa aataaagatg cagaattcat
     3421 gatggactat aatataagaa aaacagttaa agtcgcagaa ctaatccctt attggtgggg
     3481 agaagaacgt gcttctaagt ttaatgagtg atagaagagt cagtataatt ctggctcttt
     3541 ttactataag ttgaaaaaat aaaaaaaatt agaaaaagta gttgacaaag tttgaaaag
//
LOCUS       DAVLSL010000158         3569 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLSL010000158 DAVLSL010000000
VERSION     DAVLSL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.160"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(83..1498)
                     /locus_tag="VXN30_001870"
     CDS             complement(83..1498)
                     /locus_tag="VXN30_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1358444.1"
                     /translation="MTIFPDDFLWGGAVAASQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     EYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1511..3349)
                     /locus_tag="VXN30_001871"
     CDS             complement(1511..3349)
                     /locus_tag="VXN30_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1358445.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEVGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3367..>3569)
                     /locus_tag="VXN30_001872"
     CDS             complement(3367..>3569)
                     /locus_tag="VXN30_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1358446.1"
                     /translation="RLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNY
                     LLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1190 a          736 c          476 g         1167 t
ORIGIN      
        1 ttgatgtctt ccaaacctct ttatttcata tttaatgaga tgttgcctta accgtgataa
       61 tatctaatca tcaataaaca aactatttga ataaggattc tccatttgat tcaatcactc
      121 ctttatacca agtaaaggac attttcttat atcgattgaa tgtgccattt ccatcatcat
      181 ttcgatcaac ataaatgaga ccgtacctct tagaaagctg tgcagtggac atagaaacgc
      241 agtcaataca tccccaagac gtatagccca taatttccac accatcttgt agagcttcag
      301 ctacttgcaa taaatgttct ttcatatact gaattctata atcatcttgg actgttaagt
      361 tattaaattc atcttttatt agttgatctt tagcacctaa tccgttttcc actataaata
      421 atggaatttg ataacggtca taatatctat ttaaaattat acgtagtcca attggatcaa
      481 tttgccatcc ccactcagaa gactctaaat aaggatttac taagccacca ataatattcc
      541 cttctcctga attatactgt gttggaagag cagactgagt cacactcatg taatagctaa
      601 aggataaaaa atctacggta taatttttta ataactctgc atcttcagct gcaaactcta
      661 tgttaatgtc atgttcctta aaatatcttt ttgcataatt cggataataa cctctaacat
      721 gcacatctga aaatagataa tttagattct catactcatg agtcgcccat acatcttttg
      781 gatttggagt cattggataa gctggcatag ctaataccat acaccccacc ttaaactctg
      841 aattaatctc acgagcaatt tttgtaacca aactcgaggc gactaattca tgatgtatag
      901 cttgatataa ttcttgtttc gaaagattct ccttaggtat atctattcct ccactagtaa
      961 atggtaattc caaaacagag tttacttcgt taaatgtaag ccaatattta actttatctt
     1021 tatacctttc taaaactgtt cgagcaaatt tttcataaaa atgaatcatt ctcctatcaa
     1081 cccatccatg atattttctt gctaaatata atggagtctc atagtgtgaa agagttacaa
     1141 gtggctctat cccgtgagca tgtagttcat caaacaattc atcataatat tccaaaccag
     1201 cttcgttagg ttcttcttca tctccttttg gaaaaattct actccatgca atagaagtac
     1261 gaaaaacatt aaagcccatt tcagaaaaca aggatatatc ttccttatat ttatgataaa
     1321 aatcaatacc taacaatttt aagttatctt ctgtaggatt ttctgttgct tctcctaatc
     1381 cacctttggg taacacatcc tgaactgata atcccttacc atcttcatta tatgctccct
     1441 ctacttgact agctgcaaca gctccacccc aaagaaaatc atctggaaaa atggtcataa
     1501 ctttcctcca ttataatatt accagtaatt ccttagaatg ctcgattgtc tgattattag
     1561 gtaatactaa tacatctaga aaatcattgg tattcgttac aattactggt gtaactgttt
     1621 cgtagccttt agtcttgatt aaattcaagt ccatttcaaa aatcaactga tttttgaaaa
     1681 ctctgtctcc ttcttctaca tgactaataa aaccttgacc ttttagctca acagtatcta
     1741 atccaatatg aattagtaac tcaacaccct catcactctt caatccaatt gcgtgcttag
     1801 tcggaaaaat atttgtaatt ttcccatcaa atggtgcata aaccttacct tcacttggga
     1861 taatcgctac tccgtctcca attagtttat ctgaaaatgt tttatcctgg acatcgctta
     1921 acggaatgat ttctcctgat ataggagaaa atatcatttt tttatttgaa actccaactt
     1981 caacttctaa attgctagaa ctctcttctt catcgattcc aaatatataa gctaatacaa
     2041 aggtaataac aaccgaaatg accgccacaa ttaaagcatt tacaatattt gatggcacat
     2101 cagaataaat aaattgaggc aacgctatca aagatgggac agcaaataga tacgctttaa
     2161 cactagtaag acctgcaaat aatcccgcta atccaccacc aatcatagct gcataaagcg
     2221 gttttttata ttttaaagtc acaccatata atgcaggttc ggtaatccct gcaagtaagg
     2281 ctgagaaacc tgctgcaaaa gcaatttgtt ttgtattatt atttttactc tttaatgcaa
     2341 cagccatcga agcagcccct tgagctaagt ttgaccctaa cattgctgga agaattaata
     2401 cgtctggagt agcaatagat gccgccaaaa aaataggtgc aaaagcccaa tgcattccag
     2461 tcataacaat aaatggcata atagcaccaa gaatagctaa tgtaagccat ccagctacac
     2521 catacatttg cccaactaga tttgataatc cttcaccaac aattactcca ataggcccga
     2581 ctacaactaa ggcaatacag cttgatacta ataatactag cgtaggttgc aaaaaactct
     2641 tagtaacagc tggtgttaat ttagcaatca ttttttcaat atatttcatc aaccaaacca
     2701 taataagaat tggaacgact gacgaaccat aactagctgg tgtcacaggt acaccaaata
     2761 aactaagagg attccctgat tgcaccattt gaacaaaatt tggatggaga agtacacctg
     2821 ctacagacat agctaatgta gatgttactt ttaatttttg tgatgcagaa taagctaata
     2881 acagcggtaa gaaataatat ggagcatccc caaaaaatgt caaaaaagca atagtctgag
     2941 aatctgattg caatatacca agcattggta aaataattac caagactttc agcatacctc
     3001 cccctaacat tgctggaatg attggagtca tggaaccagc gatatactca atgattcttt
     3061 ctaaaatatt ccctttgtgc ccttgaacaa ctgaatcgga ttcaaaattg ccaagtttaa
     3121 cgaattcttt ataataatta gctacatcat taccaagtat aatttgatat tgtccattct
     3181 ttttcataat acctattaca cctggtatct tcttcacatc atcatcattg actaaatttt
     3241 catcttttaa ttctaatctt aaacgtgtta cacaatgggt aactctattg acattttttt
     3301 cacctccaat tacatcgagg attttttgta ccgtatcttt ataactcatg gtattctcct
     3361 attctattaa tctaaatttt ttgttaagcg acgaatatga gccatcaaat aaactaattc
     3421 actagaagtc agcaaataat tgtactccgt ttgtataaac attgccacct gttcaccaca
     3481 ttcatattct ctaggatatt tatttttcat taatgctaac aagtcttcat catcatcgtc
     3541 acaataagtt gtattttcaa ccaagcgat
//
LOCUS       DAVLSL010000159         3543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLSL010000159 DAVLSL010000000
VERSION     DAVLSL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.161"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..679)
                     /locus_tag="VXN30_001873"
     CDS             complement(<1..679)
                     /locus_tag="VXN30_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1358447.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNSRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYVEIVKL
                     LLASEGKQTGITIIEKPIIRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDACLY
                     GDGYDFQVDIQEHSYLAEVKGIRKPKGRVRLTAYEFEKAKEFQSDFILSLVTNLDDIP
                     KLVLVDNP"
     gene            complement(749..3400)
                     /locus_tag="VXN30_001874"
     CDS             complement(749..3400)
                     /locus_tag="VXN30_001874"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1358448.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAINPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(3412..>3543)
                     /locus_tag="VXN30_001875"
     CDS             complement(3412..>3543)
                     /locus_tag="VXN30_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1358449.1"
                     /translation="SKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN"
BASE COUNT          981 a          795 c          726 g         1041 t
ORIGIN      
        1 gaggattatc aactaatact aactttggaa tatcatctag gttggtgacc agagataaaa
       61 taaaatctga ttgaaactct ttagctttct caaattcgta ggcagttaaa cggacacgac
      121 ccttaggttt tcgaatccct ttaacttcgg caaggtagga atgttcttga atatctactt
      181 ggaaatcata tccatcacca taaagacatg catcggttaa ctgtccacct tgaaattttt
      241 cttctttatc aaaatgaagg ataaaataat tctctgcctc catccccgtt tcctgtagac
      301 gtttgaattt agttcgaata attggttttt caattatagt aatgcctgtt tgctttcctt
      361 cactagcaag taacagttta acaatttcaa cataactatg aacatcttca tttccaaaca
      421 tcatatcaat taaatcttta cgaaaacggt aaacttcagc tttctgccac caaccttttc
      481 gactattgtc aaaataagga tcaaataaat ccattctatt ttttacaact ccagttgttt
      541 cagcaattcc cagagaaacc acatagcgat aaaactccga cttgctagag cattgaaatt
      601 cttttataaa aagcttgtca aacttggcaa gaccgtagcc aattaaattt aacaattcat
      661 aatgagggtg tttggacatc actttctcca tcaatagata ttgatttcat tataccaaaa
      721 aacacggctc atcaccgtgt ttctgtgttt atttcaccaa cttcttcatc tcatcaatac
      781 gtgctacggt cacatcatac ttggcttggt agtcggcttg tttgtctttt tctttttgga
      841 caacttctgg tttagcgttg gcgacgaagc gttcgttgct gagttttttg ccaaccatgt
      901 cgagttcttt ctgccatttg gcaagttctt tttccagacg tgccaattct tcttcgacat
      961 tcaagaggtc taccagtggc aagaagattt cggctcctgt aataacgctt gacatagcca
     1021 gttcaggtgc agggatggtt gatgcgattt ccaagtgttc tggatttgta aagcgtttga
     1081 tatagttgac attgctgtta aagaaggctt ccaagtcgct atcgcttgtc ttaacaagga
     1141 tggtgatagg cttgcttggt gctacattta cttccgcacg cgcattccga acagcacgaa
     1201 tcaagtcttt gagactttcc acaccagtgt gagccgcaag gtcttcaaag gctagattaa
     1261 cagttgggta tgcagctgtc acgatagaac cttctgagat ttgtccaaag atttcctctg
     1321 tcacgaatgg catgattggg tgaaggagac gaaggatctt gtccagcgta tagaggagaa
     1381 cagatcgagt aatgacctta tcgtcttcat tgtcgctgta tagaacttcc ttggtcaact
     1441 caacatacca gttggcaaat tcttcccaga tgaagttgta aaggatatga ccagccacac
     1501 caaactcgaa cttatcaaag ttttcagtaa cttttgcaat ggtttcgttg agattgtgga
     1561 gaatccagcg gtccgtcaca ttaccagcct cacctgttgc aacttttgtg acattgtcat
     1621 gcgccacatc cagcgtcaaa ccttcattgt tcatgaggat atagcgagaa atgttccaaa
     1681 ttttgttaat aaagttccat gaagcatcca ttttctcgta agagaaacga acgtcttgac
     1741 ctggtgcgga accgtttgaa aggaaccaac gaagggcatc agcaccgtat ttctcgatga
     1801 catccattgg gtcaatcccg ttaccgagag atttagacat cttgcgtcct tgctcgtcac
     1861 ggatgagacc gtggataagc acgttttgga atggctgacg accagtaaat tccaaggact
     1921 ggaagatcat acgagatacc cagaagaaga tgatgtcgta acctgttacc aaggttgaag
     1981 ttgggaaata acgtttaaag tcttctgagt cgacttcagg ccagcccatg gttgaaaatg
     2041 gccagagggc agaactgaac caagtatcca agacgtcttc gtcctgagtc catccgtcac
     2101 cttctggagc ttcttcgccg acatacattt caccatcagc attgtaccag gcagggattt
     2161 ggtgacccca ccaaagctga cgagagataa cccagtcgtg gacattttcc atccattgaa
     2221 ggaaggtatc gttgaaacga ggtgggtaga attcgacctt gtcctctgtg tcttggttag
     2281 caatggcgtt cttagccaat tggtccatct tgacgaacca ttgagtagac aagcgtggct
     2341 caactacgac acctgtacgt tctgagtgac caacactgtg gacacgtttt tcgattttga
     2401 caagggcacc gatttcttcc aacttagcaa cgactgcctt acgagcttca aaacgatcca
     2461 tgcctgaaaa ttcaaaggca agctcattca tagttccgtc gtcgttcatg acgttgactt
     2521 gtggcaagtt atgacgttgg ccaaccaaga agtcatttgg atcgtgggca ggtgtgattt
     2581 tcacgacacc agtaccaagc tcaggatctg cgtgctcatc tccaacgatt gggatgagtt
     2641 tattagcgat tggaaggatg acgtttttac caatcaagtc cttgtagcgc gggtcttctg
     2701 gattaatcgc aaccgcaacg tccccaaaca tagtctcagg acgagttgta gcaacttcaa
     2761 gggcgcgtga accatcttcc agcatgtaat tcatgtggta gaaggcacct tctacatcct
     2821 tgtgaatcac ctcaatatca gaaagggctg tgcgagctgc tgggtcccag ttgatgataa
     2881 actcaccacg atagatccag cctttcttgt aaaggttcac aaagacctta cgaacagctt
     2941 ttgacaaacc ttcatcaaga gtgaaacgct cacgagaata gtctacagaa agccccatct
     3001 tgccccattg ttccttgatg gtagtggcat attcgtcttt ccattcccag accttcgtca
     3061 agaaagactc acgacctagg tcataacgcg taataccctc accacgtaag cgctcctcaa
     3121 ccttagcctg agtcgcaata ccagcgtggt ccatacctgg aagccaaagg gtatcaaagc
     3181 cttgcatgcg tttttgacgg atgatgatat cctgcaaagt cgtatcccaa gcgtgaccaa
     3241 ggtgaagttt cccagttacg tttggtggtg gaatcacgat tgaataaggc ttagcctttt
     3301 gatcgcctga aggcttgaaa acatccgcat caagccattt ttggtaacga ccagcctcaa
     3361 cctcggctgg attgtattta gatgaaagtt ctttagacat gtgtgttctc cttaattact
     3421 tttattgaca attttaacca aaccaatttt tgaaagttca atataaatct cttcaatttc
     3481 agcaaatgct gcaagtggtg acatagtata atttccagga ttatatttaa gatcatactt
     3541 gct
//
LOCUS       DAVLSL010000160         3520 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLSL010000160 DAVLSL010000000
VERSION     DAVLSL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.162"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            22..1431
                     /locus_tag="VXN30_001876"
     CDS             22..1431
                     /locus_tag="VXN30_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1358450.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1449..2744
                     /locus_tag="VXN30_001877"
     CDS             1449..2744
                     /locus_tag="VXN30_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1358451.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2749..3057
                     /locus_tag="VXN30_001878"
     CDS             2749..3057
                     /locus_tag="VXN30_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1358452.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3054..3362
                     /locus_tag="VXN30_001879"
     CDS             3054..3362
                     /locus_tag="VXN30_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1358453.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1185 a          600 c          685 g         1050 t
ORIGIN      
        1 ataatagaaa ggaggttgag catgaaaaaa aagttagtat ttcctaatct gttttggtgg
       61 ggagcagctt ctagcggacc tcagacagaa ggtcaatatg gaaaagtaca tgaaaatgtg
      121 atggactact ggttcaaaac gcatccagaa gattttttcg ataatgtcgg acctcttgta
      181 gccagtaact tttttcatac ttacaccgaa gatttccact tgatgaagga aattggagtt
      241 aattctttcc gcacttccat ccaatggagt cgactcatca agaatttaga gacaggtgag
      301 cctgatccaa aaggtattgc tttctacaat gccatcattg aagaagctaa aaagaaccag
      361 atggatcttg tgatgaattt acatcatttt gatttaccag tggaacttct tcaaaaatac
      421 ggtggttggg aaagcaaaca tgtagtggag ttattcgtga agtttgccaa gactgctttc
      481 acatgctttg gagataaggt tcattactgg acaactttca atgagccaat ggtcattcca
      541 gaagcaggat acttatatgc tttccattat ccaaatctaa aaggaaaggg aaaagaggcc
      601 gtacaagtca tctataatct aaaccttgct agtgcaaaag tgattcaact atatcgctca
      661 ttagaacttg atggaaagat tgggattatt ttaaacttga cacctgctta tccaagaagt
      721 aattctccag aagacttaga agcaagtcga tttacagatg acttctttaa caaagtcttc
      781 ttgaatccag ctgttaaagg aactttccca gaaagattgg taaaacagct agagagagat
      841 ggcgtgttat ggagtcatac cgaaaaagag cttcaactga tgaaatcaaa tacggttgat
      901 tttcttggag taaactacta ccatccaaaa cgtgttcaag cacaagcaaa tcctgaggaa
      961 tatcagacgc cctggatgcc agaccaatac ttcaaagagt atgaatggcc ggagcgtcgc
     1021 atgaatccat atcgtggttg ggaaattttt ccgaaatcca tttatgatat tgctatgatt
     1081 gtgaaggaag aatatggtaa tatcccatgg tttatcagtg aaaacggaat gggtgttgaa
     1141 aacgaagcac ggtttatcga tgaaaatgga gttatcgatg acgtgtatcg tattgaattt
     1201 tatgaagaac atttaagatg gctacataaa gccattgaag agggaagtca ctgttttgga
     1261 taccacgctt ggaccgcatt tgattgctgg tcttggaata atgcatataa gaatcgttac
     1321 ggatttatct ccgttgattt agaaacgcaa aagagaacca tcaagagctc aggaagatgg
     1381 tatcgcaaag taagtgacaa taacggtttt gaagtagaaa ttgaggagta aagagaggag
     1441 atattcatat gaaaaactca aaatttatag accaatttgc cacctttgct ggtaaactag
     1501 ggaaccaaat tcatttaaaa accctaagag atgcattcgt aacagtaatg ccattatata
     1561 ttttggcagg tttaatcgtt cttttgaaca acacggtatt taagtggatt ttccaagggg
     1621 atacattaac aagattccaa tattggggaa taacaattgc aaacggtact ttaagtattt
     1681 caggtatgat tattgctgta atggttggtt atttcttagc taaaaacaga gatttcgaaa
     1741 acccgttagc agcatcaatg ctatcattag tttctttaat tgtgatgatg ccaaatacag
     1801 tttctgtagt tcctgacgga gcaaaagatg ctgtaaacat ttcaggtgtt ctttcattca
     1861 acaacacagg tacaggcgca atgttcgccg gggttatcgt agcgattatt gcaacagaat
     1921 tattcattga attatcaaat gttaaagctt tacaaatgaa ccttggtgaa aatattccac
     1981 cagctgttaa tagatcattt agcgtattac ttccagtcat gaccgtcatc tccttatttg
     2041 gggttgtttc agcattatta ttcaatataa ctggaatgaa cttaatctca atcattacaa
     2101 tctttattca agaaccaatt cgtcatattg gtacaagctt aatcggggtc attattattt
     2161 actctttagg aaatatgtta tggctatttg gtattcacca agcagttatt tacagtgcca
     2221 tcctagaacc attactatta attaacatta ctgaaaacat cactgcagca aataatggac
     2281 aagccattcc acacatcatc aacctatcac aaatacaaac attcgcttta atgggtggta
     2341 gtggatctac attatgttta ttaatagcaa cattcttagt gagtcgcaat gctgtctcta
     2401 aaaacgtggc taaattatct tttggacctg gtatcttcaa tatcaatgaa ccagtattat
     2461 tcggttaccc aatcgtttat aacatttcat tagctattcc atttatcaca gttccagtcc
     2521 ttggtatttt aatcagctac ttagcaacag ttacaggatt catgagtcct gcatttatac
     2581 aagttccttg gactacacca gtattcttaa atgcatggtt agcaacagca ggggacgtga
     2641 gagcagttct agttcaattc atcatctttg cacttggagt tcttctatac attccattta
     2701 tcaaagttaa tgacaaagtt gttgaacaag aaatggaagg ttaagattat gaaacaaatt
     2761 ttattagtat gtaatgcggg aatgtcgaca agtatgcttg ttaaaaaaat gcaacaaagt
     2821 gcgactgaac gtgggattga aatctctatc caagcaaaat caatgacaga agctaaaaag
     2881 aatatccatg aagcagatgt cattctaatt gggccacaaa ttcgttatga gttacttgct
     2941 gtgaaagaaa ttgcaggaaa tatccctgta gataccattg atatgcggga ttacggaatg
     3001 atgaatggag caaaagttct ggaacaagca cttgcatgga taggagaaat aagatgacag
     3061 aagaaatgga atatatctgt tttcagctca ttgccaatag tggagcagcc aaatcatcct
     3121 ttattgaagc catccaacta gctaaggcag gaaatttaaa agaagcgaaa attaaagttg
     3181 aagaagcgga agactcctta gtagaagcgc acaagattca ctctaatctc atacaaaaag
     3241 aagctactgg agaaaaaata ggattctcct tgctatttat gcatgcagaa gaccaaatgg
     3301 cttcaacaga gattattcag ttgctatcta aagaattcat cgatctctat caaaataaat
     3361 aggaacactg tcattcccat ctgtctttat taatgacgac tacattgtag gaacctccaa
     3421 aagttggaca aaatgattga aaggattgga tcctgcatta cacaagatcc aatccttttc
     3481 ttttactcta gtttttataa tatgttgaat ctagaatagt
//
LOCUS       DAVLSL010000161         3460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLSL010000161 DAVLSL010000000
VERSION     DAVLSL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.163"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..1611
                     /locus_tag="VXN30_001880"
     CDS             <1..1611
                     /locus_tag="VXN30_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1358454.1"
                     /translation="DGFISSMPAVLFSSIFILIAAVPNIFGFKWSDEQLAFILKPYNY
                     SMGILALLVAGTTAKSLTDSVNTRSMEKTNQINYMSTFLAAVVGLLILAADPIEGGFA
                     NGLLGTRGLLTAFLAAFITVNIYKVCIKNNVTIRLPEEVPPNIAQVFKDVIPFALSVL
                     SIYGLDLIVRNIFGTNVAESVGKILAPLFSATDGYIGLAIVFGAYAFFWFVGIHGPSV
                     VEPLIVAISYANIEANVQLVQAGMHADKILNPVTQTFVVTMGGTGATLVVPFMFMWLC
                     KSKRNRIVGRASVVPTFFGVNEPILFGAPIVLNPIFFIPFVTAPIINVWIMKFFVDVL
                     QMNSFSIILPWTTPAPIGIVMGTALAPLSFVLAITLIIIDTLIYYPFVKVYDHQILEE
                     ERKGNSSSELKEKVAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLL
                     ANALNKAAAEYNVPVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIK
                     LAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            1826..3232
                     /gene="lacG"
                     /locus_tag="VXN30_001881"
     CDS             1826..3232
                     /gene="lacG"
                     /locus_tag="VXN30_001881"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1358455.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDAPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            3322..>3460
                     /locus_tag="VXN30_001882"
     CDS             3322..>3460
                     /locus_tag="VXN30_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1358456.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LF"
BASE COUNT         1066 a          593 c          696 g         1105 t
ORIGIN      
        1 gatggtttta tttccagtat gccagcagta ttattctcaa gtatttttat actaatagct
       61 gctgtgccca atatttttgg gtttaaatgg tctgatgaac agttagcttt tattttgaaa
      121 ccttacaact actcaatggg gattttagct ctcttagtag ctgggacaac tgcaaaatcg
      181 ttaacagatt cagttaatac acgtagtatg gagaaaacca accaaatcaa ctacatgtca
      241 acttttttag cagctgttgt tggattgtta atactggcag ctgatccaat tgaaggtgga
      301 tttgctaatg gtcttttagg tactagagga ttacttactg catttttggc agcttttatt
      361 acagtaaata tctataaagt atgtattaaa aataatgtaa ccattagact tcctgaagag
      421 gttccaccaa atattgcaca agtttttaaa gatgttattc cgtttgcctt atctgttctt
      481 tcaatatatg gattggattt aattgttaga aatatttttg gtacaaatgt agcagaatct
      541 gttggtaaaa tattagcccc tctattttca gctactgatg gttatatcgg tttagctatt
      601 gtatttggtg cttatgcttt cttttggttt gttggaattc atgggccatc tgttgtagaa
      661 cctttaattg tagctatcag ttatgcaaat atagaagcta atgttcagct tgtgcaagct
      721 ggtatgcacg cagataaaat tttaaatcca gttactcaaa cttttgttgt aacaatgggt
      781 ggtactggtg caacactagt agttccattt atgtttatgt ggttatgtaa atcaaaaaga
      841 aatagaattg tcggacgtgc gtctgtagtt cctactttct ttggcgtaaa tgaaccaatc
      901 ctatttggtg ctccaattgt tttaaatcct atcttcttta ttccgtttgt tactgctcct
      961 attatcaatg tttggattat gaaatttttt gtagatgttc tacaaatgaa tagtttcagt
     1021 attattttac cttggacaac acctgctcca attggtattg ttatgggaac ggcgctggct
     1081 ccattgtcat tcgtcttggc tataacttta atcattatcg acactttaat ctattatcca
     1141 tttgttaaag tttatgacca tcaaatttta gaagaagaac ggaaaggaaa ctcttcatct
     1201 gaattgaaag aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc tattcttgaa
     1261 aaagcaggtg tcgatgcagc acaaaatacc atcactgaag aaacaaatgt cctcgttctc
     1321 tgtgctggtg gaggtacaag tggactcctt gcaaatgctt tgaataaggc agcagcagaa
     1381 tacaatgtcc ctgtgaaagc agcagcaggc ggctatggtg ctcaccgtga aatgttacca
     1441 gagtttgatt tggttatcct tgctcctcaa gttgcttcaa actttgaaga tatgaaggca
     1501 gaaacagata agctcggtat taaactagcg aaaacagaag gcgctcaata catcaaatta
     1561 actcgtgatg gaaaaggtgc cctagcattc gtacaagaac aattcgatta aggctaggaa
     1621 ctgtgaaaca gtctcctatc gttacggaaa tcgctatggc gaatttccta atcgccttat
     1681 taattcgtcg gtaaaaagat atcgttttta cctcctcatg tcacaattcg gtgacttggt
     1741 acaagaagtg agatggagaa ggatggctca ctgactcctc tcctctcact tttactttat
     1801 ttaaatcaag aaataggtga aaaaaatgac aaaaacactt ccaaaagact ttatttttgg
     1861 tggcgcaaca gctgcctatc aagcagaagg tgctacacat actgatggta aaggaccagt
     1921 agcgtgggat aaatatcttg aagataacta ctggtacact gcagaaccag ccagtgattt
     1981 ttacaatcga tatccagttg atctcaagct agcagaagag tatggtgtca atggcattcg
     2041 aatttctatt gcctggtcac gtattttccc gactggttat ggtcaagtaa atgctaaagg
     2101 tgttgagttt tatcataatt tatttgcaga gtgtcacaaa cgtcatgttg agccctttgt
     2161 aactcttcat cactttgacg cgccagaagc tctccactca aatggagact tcttaaaccg
     2221 tgaaaatatc gaacattttg tagactacgc tgccttctgt tttgaagaat ttccagaagt
     2281 aaactattgg acaaccttca atgaaattgg accaatcggt gatggtcaat atttggttgg
     2341 gaaattccct ccaggtatcc agtacgacct tgccaaagtc tttcaatcac accacaatat
     2401 gatggtgtct catgcatgcg cggtaaaatt gtacaaagag aaaggctata aaggggaaat
     2461 tggtgttgtt cacgccctgc ctactaaata tcctctagat cctgaaaatc cagcagatgt
     2521 tcgtgcagca gagttggaag atatcatcca caataaattc atcttagacg caacttatct
     2581 aggtcgctat tcagctgaaa ccatggaagg tgtcaaccat atcttatcag tcaatggtgg
     2641 tagtttggat cttcgtgaag aagattttac agcattagaa gctgcaaaag acttgaatga
     2701 tttcctagga atcaactact atatgagtga ctggatggaa gcctttgatg gagaaactga
     2761 aattatccat aatggtaaag gtgaaaaagg aagctctaag tatcaaatca aaggtattgg
     2821 tcgtcgtgta gctcctgact atgtatcacg cacggattgg gattggatta tctaccctca
     2881 aggtttgtat gaccaaatca tgcgtgtgaa gaaagattat cctaactaca agaagattta
     2941 catcactgaa aatggtctcg gctataaaga tgagttcgtt gataacactg tttacgatga
     3001 tggtcgtatt gattacgtga aacaacactt ggagatcttg tctgatgcga ttgcagatgg
     3061 agctaatgta aaaggttact tcatttggtc attaatggat gtcttctcat ggtcaaacgg
     3121 ttatgagaaa cgttatggtc tcttctacgt agattttgaa actcaagaac gttatcctaa
     3181 gaaatcagct cactggtaca agaaagtagc ggaaactcag attatagact agtagaatta
     3241 gtcattagat atagaatttt agtgagtcaa aaagatgttc aaagatttta tccaatctat
     3301 ttatgaaaaa aagtttatat tataaatttc gaaaaatgct ctcaaatacc gtgtttgacg
     3361 agtgaagaat tgaaaagtct tggaaaatgg tatgtctcga ctggtaaaga atggatttgt
     3421 cattcagatg atgagctgga agaatttaaa aatctatttt
//
LOCUS       DAVLSL010000162         3377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLSL010000162 DAVLSL010000000
VERSION     DAVLSL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.164"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            111..2744
                     /gene="polA"
                     /locus_tag="VXN30_001883"
     CDS             111..2744
                     /gene="polA"
                     /locus_tag="VXN30_001883"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1358457.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEF
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKSYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2829..3266
                     /locus_tag="VXN30_001884"
     CDS             2829..3266
                     /locus_tag="VXN30_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1358458.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          913 a          594 c          867 g         1003 t
ORIGIN      
        1 aggtttacta aattctctgg ctctatctat ttattcggaa aaagtttgaa aaatacttgc
       61 tctagctctt cccaatggta ttttttgatt ctttccttta taatgggtgt atggataaga
      121 aaaaattatt attgattgat gggtcttctg tagcttttcg ggcgtttttt gcgctgtatc
      181 agcagttgga ccgttttaag aatgcggctg gtttgcatac caatgcgatt tatggttttc
      241 agttgatgtt gagtcatttg ttggagcggg ttgagccgag tcatattttg gtggcttttg
      301 atgcgggaaa gacgaccttc cggacagaga tgtatgcgga ctataagggt ggtcgggcca
      361 agactcctga tgagtttcgt gagcaatttc ctttcattcg tgagttgctg gatcatatgg
      421 ggattcgtca ctatgagctg gctcagtatg aggcggatga catcattggg acgctggata
      481 agctagcaga gcaggatggt tttgatatta ccattgtcag tggggacaag gatttgattc
      541 agctgacgga tgagcatacg gtggttgaaa tttccaagaa aggtgtggct gagtttgagg
      601 cctttacgcc agattacctc atggaagaaa tgggcctcac accagctcag tttatcgatc
      661 tcaaggcgct catgggtgat aagtcggata atatccctgg ggtgaccaaa gtcggtgaaa
      721 agacgggtat taagctcttg ctggagcatg gttcgcttga ggggatttat gaaaatattg
      781 atggaatgaa gacttctaag atgaaggaaa atctcatcaa tgacaaggaa caggcctttt
      841 tgtctaaaac actagcgacc attgatacca aggcaccgat tgcgattggt ttagaggact
      901 tggtctatag tggtccagat gttgaaaatc ttgggaaatt ctatgatgag atgggcttca
      961 aacagctcaa gcaggcttta aatgtgtcgt cagctgatgt gtctgagagt ttggatttta
     1021 ctattgttga ccaaatcagt caagatatgc tgagtgaaga gtctatcttc cactttgagc
     1081 tttttggtga gaattaccat acggataatt tggttggatt tgcctggtct tgtggggata
     1141 agctctatgc cacagacaag cttgagctgt tgcaagaccc gattttcaag gatttcttag
     1201 aaaaaacatc tctgagagtt tatgacttta agaaggttaa agttcttttg caacgttttg
     1261 gtgtggattt gcaggcgcct gcttttgata tccgtttggc taaatacctc ctttcgactg
     1321 tggaggacaa tgaaattgcg accatcgcta gtctttatgg tcagacttac ttggttgatg
     1381 atgaaacttt ctacggtaag ggtgttaaaa aggccattcc tgaacgtgag aaattcttgg
     1441 aacacttagc ttgtaaactt gctgttttgg tagaaacaga gcctatttta cttgaaaaac
     1501 tcagcgaaaa tgggcaatta gagcttcttt atgatatgga gcaacctctg gcttttgtcc
     1561 ttgccaagat ggaaattgct gggattatgg tcaagaaaga gaccttgctt gagatgcagg
     1621 ctgaaaatga gtttgtcatt gaaaaactga ctcaagagat ttacgagctg gccggtgagg
     1681 agtttaatgt caactcgcct aagcagttgg gcgtgcttct ctttgagaaa ttgggacttc
     1741 ctctagaata cactaagaaa accaagacag gttattcgac agcagtggat gttttggagc
     1801 gtctcgctcc tattgctccg attgttaaga aaatcctgga ttaccgtcaa attgctaaga
     1861 ttcaatctac ttatgtaatt ggcttgcagg actggatttt ggctgatgga aagattcata
     1921 ctcgctatgt gcaggatttg acccagaccg ggcgtttgtc tagtgtggat ccaaacttgc
     1981 aaaatattcc tgcccgattg gaacaagggc gcttgattcg gaaggctttt gtgccagagt
     2041 gggaggatag tgtgctactc agctctgatt attcacagat tgaattgcgc gttttggcgc
     2101 atatttctaa ggatgagcac ttgattaagg ccttccaaga gggggcagat atccatactt
     2161 cgacagccat gcgggtcttt ggcattgagc gtcctgatga tgtgactgca aacgaccgtc
     2221 gcaatgccaa ggcagttaac tttggagtgg tttatgggat ttcagacttt ggcttgtcta
     2281 ataatttggg aattagtcgt aaggaagcca aagcctacat tgatacctac tttgaacgtt
     2341 ttccaggtat taaaaactac atggatgaag tggtgcggga ggcgcgtgat aagagctatg
     2401 tagagaccct ctttaagcgt cgccgtgagt tgccagatat caattcgcgc aacttcaata
     2461 ttcgtggttt tgcggagcga actgctatca actcacctat ccagggttcg gcagcagata
     2521 ttctcaagat tgccatgatt cagctggata aagccttggt tgcaggtggt tatcagacca
     2581 agatgctgtt acaagtgcac gatgaaatcg tccttgaagt gcctaaatct gaattggtag
     2641 agatgaaaaa attggtgaaa caaaccatgg aagaagccat tcaactcagt gttcctctta
     2701 tcgcagatga gaatgaaggg gcaacctggt acgaggctaa ataaaaaggg ggctagtcct
     2761 ccttttttgt agtagaattc tgcaaacctt ttcaaaatgt gctatactga tgaaaaagga
     2821 ggatttctat gagtcaagaa tttatcaatc caagtgatgg cgtgattcgt cagtatctcg
     2881 caacgagtaa aacccttgct gtggtgggtt tgtctgaccg tgaagaaaca accagcaatc
     2941 gagtaacaaa ggaaatgcag gctcggggct ataaaatcat cccagtcaat cccaaggcgg
     3001 caggtggcga aatcttgggt gaaaaggctt atgctagcct cgctgaaatt ccttttcctg
     3061 tagatattgt caatgtttat cgtcgcagtg aatttctgcc cgatgtggcg cgtgattttc
     3121 tcaaagctga tgccaagatt ttttgggcac agctaggact tgaaagttta gaagcgaaag
     3181 agattttgcg tgatggtgga tgtgatgata tcgtgatgaa tcgctgtatc aagagagaac
     3241 atactcgctt gattgaggaa gcataagaaa aaggtagctg atgggctacc ttttgtgtta
     3301 tactcaatga aaatcaaaga gcaaactagg aaactagctg cgggttgctc aaaacactgt
     3361 tttgaggttg tagatac
//
LOCUS       DAVLSL010000163         3320 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLSL010000163 DAVLSL010000000
VERSION     DAVLSL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.165"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(229..840)
                     /gene="rpsD"
                     /locus_tag="VXN30_001885"
     CDS             complement(229..840)
                     /gene="rpsD"
                     /locus_tag="VXN30_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1358459.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1035..2087)
                     /locus_tag="VXN30_001886"
     CDS             complement(1035..2087)
                     /locus_tag="VXN30_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1358460.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2084..2782)
                     /locus_tag="VXN30_001887"
     CDS             complement(2084..2782)
                     /locus_tag="VXN30_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1358461.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2949..>3320)
                     /locus_tag="VXN30_001888"
     CDS             complement(2949..>3320)
                     /locus_tag="VXN30_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1358462.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
BASE COUNT          932 a          743 c          634 g         1011 t
ORIGIN      
        1 aataagagag aactttttta cgaaaatcta ttgaatatgc cataaaagga ttataccaca
       61 ttgtgtacta tttttggttc attttactat atcactttgg actatatcgg tctagttatg
      121 tctcaacgat tatagctgga tactaagaga aatcaaaaaa gcactaaggg agaacgtccc
      181 aaagtgctta atatcaaggc ctcaaagtct atcttattca ataaaatatt acaacatttt
      241 gttgtagaat tcaacgacaa gtgcttcgtt gatttctggg ttgatttcgt cgcgttctgg
      301 caagcgagtc aatgaacctt ccaacttttc agcgtcgaat gatacgaatg ctggacgtcc
      361 aagagtagct tctactgctt caaggattgc tggaactttc aatgattttt cacgaactga
      421 gatcacttga cctggagtta cgcggtatga tgggatatca acgcgtttcc cgtcaacaag
      481 gatgtgaccg tggtttacga attgacgagc ttgacgacga gtagtcgcga gaccaagacg
      541 gtaaacaacg ttatccaaac gacgttccaa aagaagcata aagttgaaac ctaggattcc
      601 gcctttgatt tttgtagctt gtacgaacaa gttacggaat tgtttttcac ctacaccgta
      661 agtgaaacga agtttttgtt tttcagccaa ttgcaaaccg tattctgaca atttagaacg
      721 gttgtttggt ccgtgttgtc ctggtacgta gttacgacgt gccaattctt tacctgtacc
      781 tgtaagtgaa aggccaaggc gacgagcttg tttccaagat ggtcctgtat aacgtgacat
      841 atgaatgtcc tcctgatata aataatattt cggtggaaat agtcacttag aaagccctga
      901 ttcgtgcaga tgcccttcgc ctaaacagcc aaggttactt gtcataagac acctgttgac
      961 gagcttcatg ctttcctgct gatatttcac acaaaatcta ttctaccatg aatagctgga
     1021 tttgtaaagg ggttttaggc tttattttca ctaccagaga ggttgagaac gagggtaaag
     1081 gtacttccta gaccgtactg gctgctgact gtgatttccc cacccaattg atgggccaat
     1141 tcacgcgcaa tcgcaagtcc taatccatga ccacctgtct tcatgttacg cgaagtttcg
     1201 acacgataaa gacgtttgaa aatattttcc aaatcctctg gggcaatacc ctgcccttca
     1261 tcggtcacac tgattgaaag ctggtccttc tccagcttag ccaccacttc cagcttggtt
     1321 cctggagcag aatatttaaa agcgttatcg accagattca ccaagatacg agaaagctta
     1381 gcataatctc cctcaatccg ggcagactct gggattacct gcaagtggac atctcttctc
     1441 tcctgctcaa tcaaaaactg aaattcactc atgcactcaa ttaagagctt gtccagaaaa
     1501 atactgtctt tactggtagt ttccacctga ttcctagctg tgtttagggt caaaaaattc
     1561 aactcctcaa ccagtttatt gagcctctcc gtctggcgtc caatggttgc tagataatga
     1621 gcttgctccg actccttgat aatcccatcc aaaatccctt ctaccgtcgc ttggatcgaa
     1681 gtgataggag tcttaatatc atgcgacaac tgggcaatca tcaagccctt ttctcgttcg
     1741 ctttcttcca aggaatcaaa gcttacctgc aaatcatggg acatctcatt aaaagtttgc
     1801 cctaattgct gaaattctac aggaccttga acctccaaat ttgaaggaaa atccttggcc
     1861 gctacccgct tggcatgctc cttgagtttg cccaacgacg taaagactgg caataggaga
     1921 aagagactaa tcccagcacc gacaaggctg gcaacgatgg tcatcccaag caaaaagtaa
     1981 atctcgcctt tcgcaatcag cattttttga acagcccaaa aaacaaccaa aatggttaag
     2041 agggttgaaa taatatatcc aaccaaaata taacttttta gtttcatgtt tgtcctctcg
     2101 gtttctctat cttatatccc aacccccaaa ctgtcttaat agtgggcgtt tggtcactac
     2161 tatattttgc cagctcctgt cgaagagcat ggatatgcac attcaaggta ttggtgtcat
     2221 ccacgtagtc ttctttccag atcttttcat agaggtctgt cttggagaaa actcgctctg
     2281 gattgctagc taaaatccac agcaattcaa atgatttaac agttaaatcc agcatttctt
     2341 ctcctatttg aacttcatga ctactatgat tcatttttag attgccaagg gaaatcagct
     2401 ctgtttcgcc cccacgatga aggcggcgca aaatattgtg gacacgcaaa accagctcac
     2461 gtgggctaaa aggcttagca ataaaatcat ctgctcccaa gctcaggccg taaatcttgt
     2521 cctgttcact ggtcttagca gtaataaata ggaaaggctg ctctggtgat aagtattgaa
     2581 cctcactgat taaatcataa ccatccatcc gaggcatcat gacatctgtg ataatcaaat
     2641 caatcggttt tttcttgaac agctctagcg cttccagtcc atcatgggct accaagacct
     2701 gataacctgc ctgaattaag tatctctgat gaatatctgt gatttctacc tcgtcgtcaa
     2761 cgagtaaaat tgtctttccc atctgtctct cctttgataa aaacagtgct atactgcttt
     2821 aagtataaca ctatttttat tgttttacga tgattttaat ctgctattga aagaaagtcc
     2881 tgattgctga ttgagttgtt aaactaatct tagtcagccc actatccatc atttatttcc
     2941 ttagctgatt agttttcttc ttttttgttt ttcaaaccta gaccaccgag taaacctgca
     3001 agcgcaagcc caaggaaacc aatacttgcc attgatgttt gagtctcacc agtatttggt
     3061 agcatagctt tatcctctga catcatcgta tcagacatct tgttagcaga agcagccatg
     3121 ttttcacctg ccatcgtgtt ggtagaactt gtcatggtgt cagcaggcat gctatctgta
     3181 gtacctgtag catgattgtg attcatcgga gtcacgccag aaccagagtt agaaggtgat
     3241 aatgaaccat ttgctgtgtc tgatgtttct ttaacattta tcttaatagt gactttttta
     3301 gttgctacga tgttgtccaa
//
LOCUS       DAVLSL010000164         3280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLSL010000164 DAVLSL010000000
VERSION     DAVLSL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.166"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(164..418)
                     /locus_tag="VXN30_001889"
     CDS             complement(164..418)
                     /locus_tag="VXN30_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1358463.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
     gene            complement(438..2279)
                     /gene="typA"
                     /locus_tag="VXN30_001890"
     CDS             complement(438..2279)
                     /gene="typA"
                     /locus_tag="VXN30_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1358464.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            2441..3166
                     /locus_tag="VXN30_001891"
     CDS             2441..3166
                     /locus_tag="VXN30_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1358465.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
BASE COUNT          979 a          723 c          617 g          961 t
ORIGIN      
        1 aatatgcaac cagtactaac caaatataaa atagattcca ttaacgaatt ttattcaagt
       61 ttttcccatt catattatcc aaacaaaaga gatggtgata actcaaaaat aaattgcaat
      121 cttgtaaaat tcctagcaaa aaagagagcc aaaactctct tttttatctt cttttacttt
      181 ttttgactgg catgagtgta atgtctctaa cactaaagta agctaggagc aacatggcta
      241 ttgctaggaa tatttctgtt ggtaattgaa aaattttcag aaaagataga accaataaaa
      301 tcaagagtgc cactaaaata cataccatag cgacgatatt gactgtccct ttaatgcttt
      361 ctggtgtcgc aaatacatag agtaggagca gtaaaattcc taggactaaa tagaccatct
      421 ttctctcttt ctagctctta ttcagctgat tttttcttct tgttagcttt ctcacgctct
      481 gctttgttaa ggatttgttt acgcaaacgg atagactcag gcgttacttc catgtactca
      541 tcgtcgttca agaactcaag agactcttca agtgtcaaga tacgaggcgt cttgataaca
      601 gctgtttggt ccttagtagc tgaacgaacg ttggtcattt gttttgcctt cgtgatgtta
      661 actgtcaagt cattttcacg agagttttca ccgatgatca ttccttcata aacctcagta
      721 cctgggttga caaagatcgt accacgttct tcgatagaca tgattgagta agttgtagcc
      781 ttaccagcat cgatagaaac aagggcacca cggtgacgtc caccaatttc ccctggaatc
      841 aatggcaagt attggtcgaa ggtatggttc atgataccgt aaccacgagt cattgacaag
      901 aactcagttg agtatccaat caaaccacgc gctggaacaa ggaagaccaa acgagtttga
      961 ccattaccag ttgaaatcat atccaacatt tcacctttac gttcagaaag gctttggata
     1021 acagaccctt ggtattcttc tggagtgtcg atttgtacac gttcaaatgg ttcacattta
     1081 acaccgtcga tttcttttac gataacttct ggacgagata cttgaagttc atagccctca
     1141 cgacgcattg tttcgataag gattgacaag tgcaattctc cacgtcctga aacagtccat
     1201 ttatctggtg aatcagttgg gtcaacacga agggaaacgt ctgtttgcaa ttctgcctgc
     1261 aagcgttctt ccaccttacg agaagttacc catttacctt ctttaccagc aaatggtgag
     1321 ttgttgacca agaaagtcat ttgaagagtt ggctcatcga tgtgtaggat tggaagagct
     1381 tctactgcat ctgtcggagt gatggtttca ccgacaaaga tgtcttccat acctgaaacg
     1441 gcaatcaagt cacccgcttt ggcttcttgg atttcacgac gttccaaacc aaagaaaccg
     1501 aagagttttg taacacggaa gtttttagtt gtaccgtcaa gtttagaaag ggtaacttgg
     1561 tccccaacct taactgtacc acggaagaca cgaccgatac cgatacgtcc aacgaagtca
     1621 ttgtagtcca aaagtgacac ttggaactgc aaaggctcat ctgagttatc tactggagct
     1681 gggatatggt cgataatcgt gtcaaagatt ggtgccatag tcgcttcttg gtcagctgga
     1741 tcatctgaca atgaagaagt tccgttgatc gctgaagcat aaaccactgg gaaatcaagc
     1801 tggtcgtcat ctgcaccaag ctcgatgaaa agttccaaga cttcatccac tacttctgct
     1861 ggacgagctg atggcttatc gattttgtta acaaccacga ttgggacaag gtcttgttcc
     1921 aaggcttttt tcaatacgaa acgagtttgt ggcatggttc cttcataggc atctacgacc
     1981 aagacaacac cgtcaaccat tttcatgata cgctcaactt ctccaccgaa gtccgcgtgt
     2041 cctggtgtgt ccataatgtt gatacgagtt ccgttgtaag caacggcagt atttttagca
     2101 aggatggtga ttccacgctc tttttcgata tcgtttgagt ccatagcacg ctctgccaat
     2161 tcagtacgtg catcaagcgt ttctgattgt ttcaataatt cgtcaaccag ggttgtttta
     2221 ccgtggtcaa cgtgggcgat aatcgcaatg ttacggatat cttctcttaa ttttgtcatg
     2281 atttcctcta taatattcaa aatttatttt ctaactgaac gattatacca taatttcaaa
     2341 taaataacat aactcaagca agtgtaaatg ttttcactcc gcttttcttt tcacgtcaag
     2401 ccttttcaaa gcaagcgact tatgataaga taggcacagt atgcgtttag ataatttatt
     2461 agcccaagaa aaaatcagcc gaaaggccat gaaacaagca ctcctcagag ggaaaattct
     2521 agtcgatggt tgcccagccc gctccctagc tcaaaatatc gatacaggac tacaagaact
     2581 cctttttcag gatcgaatca ttcaaggcta tgaacacacc tatcttatgc ttcataaacc
     2641 tgctggtgtc gttacagcca acaaagacaa ggaacttccg accgtcatgg acctgcttcc
     2701 atctgacatc cagtctgaca agctctatgc cgttggccga ctggaccgag atacaacggg
     2761 actcctcctc ttgaccgata acggtccctt gggcttccaa ctcctccatc cccaatatca
     2821 tgtcgataag acttatcaag ttgaggttaa tggacttcta acacctgacc atatccaagc
     2881 ctttcaaaag ggaattgtct ttttagatgg tactgtctgt aaacccgcaa aactagagat
     2941 tctatctgca agtccctcca tcagtcaagc ctctatcacc atttcagaag gaaaatttca
     3001 tcaaatcaag aaaatgttcc tctcggttgg tgttaaggtg actatcctca agagaatcca
     3061 atttggggac ttcacattga acccagattt agcagaaagt aactaccgcc ctttgaacca
     3121 aaaagagtta caaatcatta aaaactattt agagatgagt cgataaaaca aaaaaagctt
     3181 taaaactaaa gcttttttct tttatttacc gaaaaattaa ggcgattgct acaatccagt
     3241 taactacaga aatcacaatt cctaagatat taagaatctt
//
LOCUS       DAVLSL010000165         3260 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLSL010000165 DAVLSL010000000
VERSION     DAVLSL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.167"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            74..3193
                     /gene="phtE"
                     /locus_tag="VXN30_001892"
     CDS             74..3193
                     /gene="phtE"
                     /locus_tag="VXN30_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1358466.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSISKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1218 a          539 c          635 g          868 t
ORIGIN      
        1 cggtttagaa aactatttaa taaagtaaaa gaagttgaga aaaaacttca tcatttattg
       61 aaatgaggga tttatgaaat ttagtaaaaa atatatagca gctggatcag ctgttatcgt
      121 atccttgagt ctatgtgcct atgcactaaa ccagcatcgt tcgcaggaaa ataaggacaa
      181 taatcgtgtc tcttatgtgg atggcagcca gtcaagtcag aaaagtgaaa acttgacacc
      241 agaccaggtt agccagaaag aaggaattca ggctgagcaa attgtaatca aaattacaga
      301 tcagggctat gtaacgtcac acggtgacca ctatcattac tataatggga aagttcctta
      361 tgatgccctc tttagtgaag aactcttgat gaaggatcca aactatcaac ttaaagacgc
      421 tgatattgtc aatgaagtca agggtggtta tatcatcaag gtcgatggaa aatattatgt
      481 ctacctgaaa gatgcagctc atgctgataa tgttcgaact aaagatgaaa tcaatcgtca
      541 aaaacaagaa catgtcaaag ataatgagaa ggttaactct aatgttgctg tagcaaggtc
      601 tcagggacga tatacgacaa atgatggtta tgtctttaat ccagctgata ttatcgaaga
      661 tacgggtaat gcttatatcg ttcctcatgg aggtcactat cactacattc ccaaaagcga
      721 tttatctgct agtgaattag cagcagctaa agcacatctg gctggaaaaa atatgcaacc
      781 gagtcagtta agctattctt caacagctag tgacaataac acgcaatctg tagcaaaagg
      841 atcaattagc aagccagcaa ataaatctga aaatctccag agtcttttga aggaactcta
      901 tgattcacct agcgcccaac gttacagtga atcagatggc ctggtctttg accctgctaa
      961 gattatcagt cgtacaccaa atggagttgc gattccgcat ggcgaccatt accactttat
     1021 tccttacagc aagctttctg ccttagaaga aaagattgcc agaatggtgc ctatcagtgg
     1081 aactggttct acagtttcta caaatgcaaa acctaatgaa gtagtgtcta gtctaggcag
     1141 tctttcaagc aatccttctt ctttaacgac aagtaaggag ctctcttcag catctgatgg
     1201 ttatattttt aatccaaaag atatcgttga agaaacggct acagcttata ttgtaagaca
     1261 tggtgatcat ttccattaca ttccaaaatc aaatcaaatt gggcaaccga ctcttccaaa
     1321 caatagtcta gcaacacctt ctccatctct tccaatcaat ccaggaactt cacatgagaa
     1381 acatgaagaa gatggatacg gatttgatgc taatcgtatt atcgctgaag atgaatcagg
     1441 ttttgtcatg agtcacggag accacaatca ttatttcttc aagaaggact tgacagaaga
     1501 gcaaattaag gctgcgcaaa aacatttaga ggaagttaaa actagtcata atggattaga
     1561 ttctttgtca tctcatgaac aggattatcc aagtaatgcc aaagaaatga aagatttaga
     1621 taaaaaaatc gaagaaaaaa ttgctggcat tatgaaacaa tatggtgtca aacgtgaaag
     1681 tattgtcgtg aataaagaaa aaaatgcgat tatttatccg catggagatc accatcatgc
     1741 agatccgatt gatgaacata aaccggttgg aattggtcat tctcacagta actatgaact
     1801 gtttaaaccc gaagaaggag ttgctaaaaa agaagggaat aaagtttata ctggagaaga
     1861 attaacgaat gttgttaatt tgttaaaaaa tagtacgttt aataatcaaa actttactct
     1921 agccaatggt caaaaacgcg tttcttttag ttttccgcct gaattggaga aaaaattagg
     1981 tatcaatatg ctagtaaaat taataacacc agatggaaaa gtattggaga aagtatctgg
     2041 taaagtattt ggagaaggag tagggaatat tgcaaacttt gaattagatc aaccttattt
     2101 accaggacaa acatttaagt atactatcgc ttcaaaagat tatccagaag taagttatga
     2161 tggtacattt acagttccaa cctctttagc ttacaaaatg gccagtcaaa cgattttcta
     2221 tcctttccat gcaggggata cttatttaag agtgaaccct caatttgcag tgcctaaagg
     2281 aactgatgct ttagtcagag tgtttgatga atttcatgga aatgcttatt tagaaaataa
     2341 ctataaagtt ggtgaaatca aattaccgat tccgaaatta aaccaaggaa caaccagaac
     2401 ggccggaaat aaaattcctg taaccttcat ggcaaatgct tatttggaca atcaatcgac
     2461 ttatattgtg gaagtaccta tcttggaaaa agaaaatcaa actgataaac caagtattct
     2521 accacaattt aaaaggaata aagcacaaga aaactcaaaa cttgatgaaa aggtagaaga
     2581 accaaagact agtgagaagg tagaaaaaga aaaactttct gaaactggga atagtactag
     2641 taattcaacg ttagaagaag ttcctacagt ggatcctgta caagaaaaag tagcaaaatt
     2701 tgctgaaagt tatgggatga agctagaaaa tgtcttgttt aatatggacg gaacaattga
     2761 attatattta ccatcaggag aagtcattaa aaagaatatg gcagatttta caggagaagc
     2821 acctcaagga aatggtgaaa ataaaccatc tgaaaatgga aaagtatcta ctggaacagt
     2881 tgagaaccaa ccaacagaaa ataaaccagc agattcttta ccagaggcac caaacaaaaa
     2941 acctgtaaaa ccagaaaact caacggataa tggaatgttg aatccagaag ggaatgtggg
     3001 gagtgaccct atgttagatc cagcattaga ggaagctcca gcagtagatc ctgtacaaga
     3061 aaaattagaa aaatttacag ctagttacgg attaggctta gatagtgtta tattcaatat
     3121 ggatggaacg attgaattaa gattgccaag tggagaagtg ataaaaaaga atttatctga
     3181 tctcatagcg taaggaatag cagtagaaaa agtctgaatc aaaaatgaag ttctctcaaa
     3241 agttagaaat aaaactctga
//
LOCUS       DAVLSL010000166         3250 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLSL010000166 DAVLSL010000000
VERSION     DAVLSL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.168"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(100..183)
                     /locus_tag="VXN30_001893"
                     /pseudo
     CDS             complement(100..183)
                     /locus_tag="VXN30_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            227..2356
                     /locus_tag="VXN30_001894"
     CDS             227..2356
                     /locus_tag="VXN30_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1358467.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            2343..2792
                     /locus_tag="VXN30_001895"
     CDS             2343..2792
                     /locus_tag="VXN30_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1358468.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            2851..3123
                     /locus_tag="VXN30_001896"
     CDS             2851..3123
                     /locus_tag="VXN30_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1358469.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT          939 a          630 c          740 g          941 t
ORIGIN      
        1 gacttcgtca gttctatcca caacctcaaa acagtgtttt gagctgactt cgtcagttct
       61 atccacaacc tcaaaacagt gttttgagct gacttcgtca gtcttatcca caacctcaaa
      121 acagtatttt gagcagccta cggctagctt cctagtttgc tttttgattt ttattgagta
      181 tgaaaaggat tggagaaagc cttccagctt gtggtataat agatttatgg ataaaaaata
      241 tgaaaaaatc tctcaggatt tgggagtgac gttaaagcaa attgataccg ttctaagttt
      301 gacagctgaa ggggcgacta ttccctttat cgcgcgttat cgcaaggaca tgactggtag
      361 tctggatgag gtggcgatta aggctattat tgatttggat aaaagtctga caaatctcaa
      421 tgaccgtaag gaagctgtct tagctaagat tcaagaacaa ggtaagttga ccaaggaatt
      481 ggaagaagct atcttagttg ccgaaaaatt agcagacgtt gaagaactct atcttcctta
      541 taaggaaaag cgtcgtacca aggcaaccat tgcccgtgaa gctggactct ttcctcttgc
      601 tcgtttgatt ttgcagaata tagttgactt agagaaagaa gctgaaaagt tcgtctgtga
      661 aggatttgcg actggcaagg aagccttgac cggtgcagtt gatattttgg tcgaagcctt
      721 atcggaagat gtgaccttgc gttctatgac ttatcaggaa gtgctgagac actctaaact
      781 cacttctcaa gccaaggatg aaagtcttga tgaaaagcag gtttttcaga tttattatga
      841 tttttcagag acagttggaa ctatgcaagg ctatcgtacc ttggctctca atcgtgggga
      901 gaaacttggt gtcttgaaga tcggttttga acatgcgacg gaccgtattc ttgccttctt
      961 tgctactcgt ttcaaggtga aaaatgctta tattgatgaa gttgttcagc aatccgttaa
     1021 gaaaaaggtc ttgcctgcta ttgagcgtcg tattcggaca gaattaactg agaaagctga
     1081 agagggagct atccaacttt tttctgacaa tctgcgcaat ctcctcttgg ttgctccact
     1141 gaaagggcgc gtggttcttg ggtttgaccc agcctttcgt acaggtgcca agctagctgt
     1201 cgtggatgca acaggaaaaa tgctgacaac tcaggttatt tatcctgtta aaccagcatc
     1261 agctcgtcaa atcgaagaag ccaagaaaga tttagcagat ttaattggtc aatacggtgt
     1321 agagattatt gccattggaa atggaacggc cagtcgtgaa agtgaagctt ttgtagcgga
     1381 agttctgaaa gatttccctg aagtcagcta tgttatcgtt aatgaaagtg gtgcttctgt
     1441 ctattctgcc agcgaacttg ctcgtcagga gtttccagac ttgaccgttg aaaaacgctc
     1501 tgccatttct atcgcccgtc gtttgcaaga tcctcttgcg gaattggtca aaatcgatcc
     1561 taagtcaatt ggtgtcggtc aataccaaca cgatgtcagt cagaagaaac tatctgagag
     1621 tctggacttt gttgtcgata cagtggttaa ccaagttggt gtcaatgtca atacagctag
     1681 cccagctctt ctttcacacg tagctggact caataaaacc atttctgaaa atatcgtcaa
     1741 ataccgtgag gaagagggaa aaatcacttc acgctcccaa atcaagaaag ttcctcgttt
     1801 gggggccaag gcttttgaac aggctgctgg tttcctccgt atccctgaaa gtagcaatat
     1861 ccttgataat acaggagttc acccagaaaa ttacgctgcc gttaaggaac tcttcaaatg
     1921 cttggatatt aaggatctga atgaagaggc ccaaagcaaa ctcaagtcgc tttcagtcaa
     1981 ggaaatggcg caagagttag accttggtcc agaaactctt aaagatatca ttgcagatct
     2041 tctcaaacca ggtcgagatt tccgtgattc ctttgatgct ccagttctcc gtcaagatgt
     2101 cctagatatc aaggacttag tggttggaca aaagctagaa ggagtggtgc gtaacgttgt
     2161 tgatttcggt gcttttgttg atatcggaat tcatgaggac ggtctcatcc atatttccca
     2221 tatgagtcgc aagtttatca aacaccctag tcaagtggtg gcagttggag atttggtgag
     2281 cgtttgggtt aagaaaattg atactgaacg tgaaaaagtt aatctgtctc tcctagctcc
     2341 aaatgaaact gactgattac gtcaagcagg tttcactaga agacttcggc agacctttta
     2401 tccatcatgt ccagtggaat aggcgtctac gttcgacagg tgggcgattt ttccccaaag
     2461 atgggcattt ggattttaat cccaaggttt atcaggaact ggggttggac gtttttagga
     2521 aaattgtccg acatgaactc tgtcattatc acctctattt tcaagggaaa ggctatcaac
     2581 acaaggatcg ggattttaag gaactgttga aagcagtgga tggattacgg tttgtaccat
     2641 ccttgcccaa tagtaactcc aagccaatca agctctatcg ttgccaatcc tgccagcaaa
     2701 cttatcagcg taagcgtagg attgatacca aacgctatcg ctgtggactt tgtcgaggaa
     2761 agttgctatt agtaaatcag cctgaggact gatgaaagcc gagcctatcc gtgatatact
     2821 atgtctaacc tagcagaaag aggaaatatc atgaacagta agttttataa aatgagacga
     2881 aatcgcatga tttcaggagt tttagcaggt ctgtcagaca agtggaactt tgatgttacc
     2941 cttgtacgtt ttctgtttgc catttttaca gtcgcaaact ttggacttgg ggtgattatc
     3001 tatatcatcc tagcttctat catgccaacc aaggaagaaa tcgaagcaga aatgtacgga
     3061 acaggcccac gtaaacgcaa agaagctcaa gccattgacg ataatgaagg ctggttttgg
     3121 tgaggtttta caaaagattt ggaatacgca aaaagcttta aatcaatagt ctaaactgct
     3181 tgatttaaag cttttttact tttatactca ataaaaaacg gctctttgtc aactgtagtg
     3241 ggttgaaaaa
//
LOCUS       DAVLSL010000167         3243 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLSL010000167 DAVLSL010000000
VERSION     DAVLSL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.169"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            75..1586
                     /locus_tag="VXN30_001897"
     CDS             75..1586
                     /locus_tag="VXN30_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1358470.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1608..2939
                     /locus_tag="VXN30_001898"
     CDS             1608..2939
                     /locus_tag="VXN30_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1358471.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKETGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEICIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            3034..3161
                     /locus_tag="VXN30_001899"
                     /pseudo
     CDS             3034..3161
                     /locus_tag="VXN30_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
BASE COUNT          877 a          675 c          700 g          991 t
ORIGIN      
        1 agcgttttct tgaatgttta tttaagaaag tagttggttt tttacacttt gttagacatc
       61 aggaggaaaa acaaatgagt gaaaaagcta aaaaagggtt taagatgcct tcatcttaca
      121 ccgtattatt gataatcatt gctattatgg cagtgctaac ttggtttatc cctgcggggg
      181 cctttataga aggtatttac gaggctcagc ctcaaaatcc acaagggatt tgggatgtcc
      241 tcatggcacc gattcgggct atgctaggta ctcatccaga ggaaggttcg ctcattaaag
      301 aaacgagcgc agcgattgat gtagccttct tcatccttat ggttggtggt ttccttggca
      361 ttgtcaacaa aactggtgct cttgacgtag ggattgcctc tatcgtgaag aagtataagg
      421 gccgcgaaaa aatgttaatt ttggtactga tgcctttgtt tgccctcggt ggtacaactt
      481 atggtatggg ggaagaaaca atggccttct atccactcct tgtgccagtt atgatggccg
      541 ttggttttga tagcctgact ggtgttgcaa ttattttgct cggttctcaa atcggctgtt
      601 tggcatctac tctgaatcca tttgcgacag gtattgcttc agcgactgcg ggagttggta
      661 caggggacgg tatcgtactt cgtctgatct tctgggttac cttgactgct cttagtactt
      721 ggtttgttta ccgttatgcg gataagattc aaaaagatcc gactaagtca ctggtttata
      781 gtactcgcaa agaagatttg aaacacttta acgtagaaga atcttcatct gtagaatcta
      841 cacttagcag caaacaaaaa tcagttctct tcttatttgt gttgacattc atcttgatgg
      901 tattgagctt cattccatgg acagaccttg gcgttaccat ttttgatgac tttaatgctt
      961 ggttgactgg tcttccagtt attggtaata ttgtcggttc atctacttct gcactaggta
     1021 cttggtactt cccagaaggc gcaatgctct ttgcctttat gggtatcctg attggtgtta
     1081 tttatggtct taaagaagat aagattatct cttccttcat gaatggtgct gctgacttgc
     1141 tcagtgttgc cttgatcgta gcgattgctc gtggtattca agttatcatg aacgacggta
     1201 tgattaccga tacaatcctc aactggggta aagaaggctt gagcggtcta tcttcacaag
     1261 tctttatcgt tgtaacttat atcttctatc tacctatgtc attcttgatc ccatcttcat
     1321 ctggtcttgc cagcgcaact atgggtatca tggctccact tggagaattt gtaaatgtcc
     1381 gtcctagctt gattatcact gcttaccaat ctgcttcagg tgtcttgaac ttgattgcac
     1441 caacatctgg tattgtgatg ggagctcttg cacttggacg tatcaacatt ggtacttggt
     1501 ggaaattcat gggcaaactc gtagtcgcta ttattgtagt gaccatcgcc cttcttctcc
     1561 ttggaacctt ccttccattc ctataaaata gtgagtgagg tgattccatg aaaatagata
     1621 taacaaatca agttaaagat gaatttctta tatcattaaa aaccttgatt tcctatcctt
     1681 cagtactcaa tgaaggagaa aatggaacac cttttggaca agcaatccaa gatgtcctag
     1741 aaaaaacttt agagatttgt cgagacatag gtttcactac ctatcttgac cctaaaggtt
     1801 attacggata tgcagaaatc ggtcagggag cagagcttct ggccattctc tgtcatttgg
     1861 atgttgttcc atcaggtgat gaagcagatt ggcagacacc gccatttgaa gcaactatca
     1921 aagacggctg ggtattcgga cgtggtgtcc aagatgataa aggcccttcg ctcgcagctc
     1981 tctatgcagt aaaaagcttg ctggaccaag gtattcagtt caaaaagcgc gtacgcttta
     2041 tctttggtac cgatgaggaa accctctggc gctgcatggc acgctacaat accatcgaag
     2101 aacaggccag catgggcttt gcacctgact catcttttcc tctgacctat gctgaaaaag
     2161 ggcttctaca ggtcaaactt catggccctg gatcggatca actagagctt gaagtaggag
     2221 gcgcctttaa cgttgtacca gacaaggcca actaccaagg tcccctctat gaacaggttt
     2281 gtaacggtct caaagaaact ggttatgatt accaatccac tgaacaaacc gtaacggttc
     2341 tcggagtgcc aaagcatgct aaggatgcta gtcaaggtat caatgctgtc atccgactag
     2401 ctaccattct tgctcctctc caagaacacc ctgctctcag ttttcttgca acacaagcag
     2461 gtcaagacgg cacaggaaga caaatctttg gtgatatagc agatgaacct tctggtcacc
     2521 tatcctttaa tgtcgcaggt ctcatgatca atcatgaacg ttctgaaatc tgtattgaca
     2581 ttcggactcc tgtcttagct gataaggaag aactagtaga gttgcttaca agatgtgcac
     2641 aaaactacca actccgctac gaagagtttg actatctagc gcctctatac gtcgcaaaag
     2701 acagtaaact cgttagcaca ctgatgcaaa tctaccaaga aaagactggc gataacagtc
     2761 ctgctatttc atccggtggt gccacttttg ctcgcaccat gccaaattgt gtagccttcg
     2821 gcgccttatt cccaggagcg aagcagacag aacatcaggc aaatgaatgt gccgttctag
     2881 aagatttgta ccgtgctatg gatatttatg ccgaagccgt ctatcgactt gcaacttaat
     2941 caggcaactg tttctaccaa aaaaaatcga ccgattaatg aactgcaccc caaaagttag
     3001 acagaataaa tctaactttt ggggtgtttt attatgaaat tgagttatga agataaagtt
     3061 cagatctatg aactaagaaa gcaaggacaa agcttcaaac agctttcaaa aagatttgtg
     3121 tggatgtttc tggtctaaag tcatctgaat ctttgagatg agctttataa atcgattttt
     3181 tcagtttttg cactggtgtt tcgataaact caaacttttt agccgtggta ttgcctgatt
     3241 tta
//
LOCUS       DAVLSL010000168         3210 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLSL010000168 DAVLSL010000000
VERSION     DAVLSL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.170"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(325..1281)
                     /gene="lytA"
                     /locus_tag="VXN30_001900"
     CDS             complement(325..1281)
                     /gene="lytA"
                     /locus_tag="VXN30_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1358472.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(1657..3027)
                     /locus_tag="VXN30_001901"
     CDS             complement(1657..3027)
                     /locus_tag="VXN30_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1358473.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         1028 a          684 c          599 g          899 t
ORIGIN      
        1 gcacactctt gtcataattt tttaaataaa aaaattatga caaaacaagg aagcaattta
       61 ttgatgctgc ttaaaaatct aaaattgatg agttcctatc aaaaatcgta tcttcaacct
      121 caaaacagta cttaaagcta tccgactcgg tttacattgt caaatttaga ttttatttga
      181 gcataacttt ctagtttgct ttctgatttt tgtttaatat agtagcaaac attgaaggaa
      241 atatctccac aaaaaaacgc atactattaa gctttttcaa gacctaataa tatgcgctgt
      301 tctgatttga aagacattcc attattattt tactgtaatc aagccatctg gctctactgt
      361 gaattctggc ttgtctgcca gtgttccgtc tggtttgagg tagtaccagc ctgttccgtc
      421 cgctgactgg ataaaggcat ttgataccat ggcgccttct ttagcgtcta agtagtacca
      481 agtgtccttg tacttgaccc agcctgtctt catggcacct tcttcgttga aatagtacca
      541 cttatcagcg attttcttcc agcctgtagc catttcgcct gagttgtcga accagtacca
      601 gttgccgtct gtgtgcttcc tccagcggtc tgcaagcata tagcctgaac tgtcaaagta
      661 gtaccaagtg ccattgattt tctcaaactt gtcttttgga taagagccgt ctgaatgtac
      721 gtaccagtag ccagtgtcat tcttctgcca gcctgtttca atcgtcaagc cgttctcaat
      781 atcatgctta aactgctcac ggctaatgcc ccatttagca agatatggat aagggtcaac
      841 gtggtctgag tggttgtttg gttggttatt cgtgcaatac tcgtgcgttt taattccagc
      901 taaactccct gtatcaagcg ttttcggcaa acctgcttca tctgctagat tgcgtaagag
      961 ttcgatataa aggcggtagt ccgtcatgaa ctcttctttg gttgaatggc tttcaataag
     1021 ttcaaccgct gcataggtct cagcattcca accgccccca acgtcccagg caccattatc
     1081 aacaggtcct acctgcatga tgcaaccgtt cccaacaatg tgcgagaaaa aacctaattc
     1141 tgggtctttc cgccagtgat aatccgcttc attctgtacg gttgaatgcg gattcccagt
     1201 tgagtgtgcg tgtacttgcc tatatggttg cacgccgact tgaggcaaat ctgttcttaa
     1261 tttactcaca ttaatttcca tattctactc cttatcaatt aaaacaactc attttttaca
     1321 atccagaacc agaaactcct ttatttctac cttacaaaga agacaatctt agtcacgatt
     1381 aggcttgtag atagaacctc aaaacgcact attttgacac tgtaaatagg actgacaagg
     1441 tctgcattct atctacaata acaccccaga ctaaaaagct tttcaaaagt atatttttac
     1501 agtctctata tgtccttttc ataaatacta tactttatta tatcatataa aagaagtcaa
     1561 aagtctgtta aactattttc aacaccaaac taaagaagag aacacaagtt ttttcgatgt
     1621 tcactagagg aaatggattt tattcagtaa atccaactag gattgcactt tggttgccaa
     1681 aattgccttt ccttctttta tcaagggatg acggaatagt gagaagtaca gttgaactgt
     1741 catggcaacc cagataagag gttcacaaag gataacaccc ttatatcctg cccaaggaat
     1801 aatcaaaacc acaaaaacga tttttccgat tagttcaata aagctagaaa ctagaggaag
     1861 gatcttctgc cccaagccct gcaagcaatt gcgataaatc aacaagaggc tcaaaatggg
     1921 ataaaaggct gaactgattt gcagatagag acttccattt tctatcaagt aaccatctgt
     1981 cgaactagcc aagaaggaaa ccaaggctgg actggcaaaa aagaggaaaa tacaaacaaa
     2041 aactgcccag gatatactta aacgactgcc gattcgaaga ccttgaacaa tgcggtcagg
     2101 tcgcttagct cctagattct gagaagcaaa ggtcgtcatt gatgcagaaa tagcggtcat
     2161 aggaagaagg gcaaaggtca taatgcgtcg agctgccgtc tgggcactaa taatcactgc
     2221 accaaatgta ttaacagaaa actgtaaaat cacactaccg atagatacaa ttgaactcat
     2281 caagcccata gccaaacctt gctccaagag atccgcgtac aagcttttgt cccatttgaa
     2341 atgtttaaac tgtggcaaga gttctggcac acttttacga atataataaa agcagagaac
     2401 cgctgataaa ccttgcgaaa taatggtagc aagtcctgcg gattgaactc ccagatgcaa
     2461 ttgcgtaata aaatagagat ccagaaccac attaaccaag gcagagaaaa tcagaaatcc
     2521 cagggctgct agactgtcac caatagaccg caacaagcct gcaaaaagat tataagcaaa
     2581 gctgacacct acacaggtca caatcataga aatatattga taagattgag gaagaatttc
     2641 tgcaggagta tctaagtatt gcaagagagg atacaagcca agaaagccca gcaacataac
     2701 tagaatgctc aaaagagcac ctaaaatcca ggtggctgct actgcttcct tgattttagt
     2761 gaaattccga gccccataat aacgagcaat gacaatcccc atgccattgc caacaccaag
     2821 tgtaaaacct acaatcaggt caaaaatcgc tgtcgtcgct cctactgcag ccaaggattc
     2881 ttgaccaaga aatcgtccaa caatcaagac atcagcagtg ttatagagct gttgaaaaat
     2941 atttgatagc aagattggaa aggtgaagct taagagcgag ggaagaatcg gaccatgtat
     3001 caggtccact gttcgttttt tattcataaa gctattatat cagctttcca gaggagcgac
     3061 aagaaacagc aaacttatat atagcggaat gaaataagga cagtcaaatc gatttttaac
     3121 aatgttttag aagtagatgt gtactattct agtttcgatc tattatagtt ggttgagttt
     3181 ggaatagtac gctgtagctg ctaaaacatt
//
LOCUS       DAVLSL010000169         3172 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLSL010000169 DAVLSL010000000
VERSION     DAVLSL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.171"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            414..836
                     /locus_tag="VXN30_001902"
     CDS             414..836
                     /locus_tag="VXN30_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1358474.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            833..1063
                     /locus_tag="VXN30_001903"
     CDS             833..1063
                     /locus_tag="VXN30_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1358475.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            1524..1727
                     /locus_tag="VXN30_001904"
     CDS             1524..1727
                     /locus_tag="VXN30_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEV1358476.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            1809..3026
                     /locus_tag="VXN30_001905"
     CDS             1809..3026
                     /locus_tag="VXN30_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1358477.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         1120 a          542 c          641 g          869 t
ORIGIN      
        1 aaaaagcact actaaacgcc aataaaaaga accgttatat ggtagtgcta tttacgctgt
       61 taaaatattg tatattactt ccaaatggcg gtttgttgga ggtcaacgtc gccatgaagt
      121 acatcatata caataaattt ccttacattg ggttcttgtc aaaaaaagtc gtctatctgc
      181 aatagataag tacgtccacc aatgtggttt tataaatcat atagatagaa taacagaagc
      241 atgtaaacag agaaataaat ctgtttatat gcttttttgg ctattcagaa cttttttaca
      301 aagtttattt atcagtaatg caacaaatcc ccctttcaca ttgggactaa gagtgaaagg
      361 agataaacga gcaaggctca cttcctttcc tagacagaaa gggggtgaga aacatgaaac
      421 catcttcttt tcagaccaca atagaaaatc agtttgacta tatctgtaaa cgtgctatgg
      481 aagacgagcg aaagaattat atgctttatc tttcaaggat tgcaaagcgt gaggtgtcct
      541 tttcggatgt tggcgattat cttgttagcc agtttgcgac aacagataac tattcaactg
      601 actttcagat ttttacactc aatgggttat cagtaggcgt tgaaaatgat ttgttgagtg
      661 aagcattacg tgagttgcca gacaagaaac gtgaaattct actgctgttt tactttatgg
      721 acatgagcga ttcagaaatt gcagacctgt tgaaattgaa ccgttctact gtctatcggc
      781 atagaaccag tggactagcc ttaattaaaa agtttatgga ggaatttgaa gaatgaaaac
      841 acaatatcct atgattccct ttcctctcat tgtaaaggca acagatggcg ataccgaagc
      901 gattaaccag attctacatc attacagagg gtacataacg aagcgttccc tacgacttat
      961 gaaagatgaa tatggcaatc aaagtatggt cgttgatgaa gtcttacgtg gaagaatgga
     1021 aaccagactg attacaaaga ttttgtcatt tgaaattaag taatatcctc tctcctttcg
     1081 tggaagcgtg ctaaaccatt ccacgcttcc cgaacaggga ggtttgttat tccaccaaag
     1141 catattgagc tttcaatgtg ttttgatagg ctaacgagcc attgttcttt gaaaactgaa
     1201 taaaagtaat cgaatacgtt tcgataagaa aagagccaac ggaactaacc gccatgacct
     1261 atcttataaa gatagcgagc gattcatgtt agtgatccga gaagcaatct ttagcaggat
     1321 tgcctgcaac gacattctta tcgtgataat gatactccca tacagtcaat agtccgagcg
     1381 tgataaaacc gtcgcaggca atgagtatgg ctacatgaga accatgcagg ggtggaactc
     1441 ccgtgagctt tgctaaagct gttcgattgc tggtaaaaca acttttatga aatccaaata
     1501 agtgatttgg aaaggaggat tttatgaagc agactgacat tcctatttgg gaacgttata
     1561 ccctaaccat tgaagaagcg tcaaaatatt ttcgtattgg cgaaaacaag ctacgacgct
     1621 tggcagagga aaataaaaat gcaaattggc tgattatgaa tggcaatcgt attcagatta
     1681 aacgaaaaca atttgaaaaa attatagata cattggacgc aatctagcgt cgccaaaggg
     1741 tcttgtatat gataaaatag tattaagtcg tatcaaggct ctttccataa aggaaaggag
     1801 caaatgccat gtcagaaaaa agacgtgaca ataaaggtcg aatcttaaag actggagaga
     1861 gccaacgaaa agacggaaga tacttataca aatatataga ttcatttgga gaaccgcaat
     1921 ttgtttactc gtggaaactt gtggctacag accgagtacc agcaggaaag cgtgattgta
     1981 tctcacttag agagaaaatc gcagagttac agaaagacat tcatgatggt attgatgttg
     2041 taggaaagaa aatgacactc tgccagcttt acgcaaaaca gaacgctcaa agaccaaagg
     2101 ttagaaaaaa cactgaaact ggacgcaaat atcttatgga tattttgaag aaagacaagt
     2161 taggtgtaag aagtattgac agtattaagc catcagacgc taaagaatgg gctattagaa
     2221 tgagtgaaaa tggttatgct tatcaaacca tcaataacta caaacgttct ttaaaggctt
     2281 cattctatat tgctatacaa gatgattgtg ttcggaagaa tccatttgac tttcaactga
     2341 aagcagttct tgatgatgat actgtcccta agaccgtact aacagaagaa caggaagaaa
     2401 aactgttagc ctttgcaaaa gctgataaaa cctacagcaa aaattatgat gaaattctga
     2461 tactcttaaa aacaggtctt cgtatttcag agtttggtgg tttgacactt ccagatttag
     2521 attttgagaa tcgtcttgtc aatatagacc atcagctatt gagagatact gaaattgggt
     2581 actacattga aacaccaaag accaaaagtg gcgaacgtca agttcctatg gttgaagaag
     2641 cctatcaagc atttaagcga gtgttagcga atcgaaagaa tgataagcgt gttgagattg
     2701 atggatatag tgatttcctc tttcttaata gaaagaacta tccaaaagtg gcaagtgatt
     2761 acaacggcat gatgaaaggt cttgttaaga aatacaataa gtataacgag gataaattgc
     2821 cacacatcac tccacatagt ttgcgacata cattctgtac caactatgca aatgcaggaa
     2881 tgaatccaaa ggcattacag tacattatgg gacatgctaa tatagccatg acgctgaact
     2941 attacgcaca tgcaacattc gattctgcaa tggcagaaat gaaacgcttg aataaagaga
     3001 agcaacagga gcgtcttgtt gcttagtagt acaaatgaat ttactactta tttaccactt
     3061 ctgacagcta agacatgagg aaatatgcaa agaaacgtga agtatcttcc tacagtaaaa
     3121 atactcgaaa gcacatagaa taaggcttta cgagcattta agaaaatata aa
//
LOCUS       DAVLSL010000170         3137 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLSL010000170 DAVLSL010000000
VERSION     DAVLSL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.172"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     regulatory      complement(94..182)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(391..1020)
                     /locus_tag="VXN30_001906"
     CDS             complement(391..1020)
                     /locus_tag="VXN30_001906"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1358478.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGVKLMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(1022..1804)
                     /gene="thiM"
                     /locus_tag="VXN30_001907"
     CDS             complement(1022..1804)
                     /gene="thiM"
                     /locus_tag="VXN30_001907"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1358479.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALSVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(2093..2761)
                     /locus_tag="VXN30_001908"
     CDS             complement(2093..2761)
                     /locus_tag="VXN30_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1358480.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLIMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(2976..3064)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          973 a          673 c          449 g         1042 t
ORIGIN      
        1 atcttcatcc agtttcgtaa aagaaaaaac tctaattaca gatacgaatt agagttcagc
       61 ttacaagatt agacagttct tttcgacata cgaaaaaaac atttcacatt tcccttcgcc
      121 agtcttaact gtatcaggtt caatgggtat catctcagcc taaagcaccc caaatgtctt
      181 tattatttaa ttatgtgatt attataacac acattttata ctagttcaag aaattgaact
      241 ggaaatacag ccttgcactc acaaagacag cagatctttc ttttgcaaaa aacaaatgac
      301 ctgtttgatg aattagccat tcaagctgaa tctagacata gctttttaaa aaaggaaaat
      361 cctacttact tagaatccaa ggatagatat ctattgttca ctcatttccc gaacagtttt
      421 ttctatattt tttgcatacg atattgccga aatgattgaa acgccatcca tattggtctt
      481 tataatgtct ttaatatgtt tcgtctgtat cccaccaatt gcaactaagg gcatttgtgg
      541 caacagtttt ctcatcaatt taacaccttc ataacctata gcaccaccag catcatcctt
      601 tgactgggta tcaaatacag gcccaacacc aacataatct acatattcaa cttttgattg
      661 ttgaaattct tcctcgtttt ttatagaaag accaattatt ttatctggca tcaattttct
      721 aatttcatca acaccaatat catcttgacc tacatgtacg ccatcagcgt caatttccat
      781 tgctaaatct atatcgtcat taacgataaa tggaacattg tattttttac aaagttcttt
      841 aatttggata gctagctcaa gtttttctaa gccttctaaa gcaccctcac ctttttctcg
      901 aaattgaaat aaggttatac caccttttaa ggcttcctca acgactgtat atagattttt
      961 tccttggcaa gtagtcgttc cacaaataaa atatagtttt agtaattctt tatgaaacat
     1021 cttacttcac tcttttgaat tcctttacat cttcatctgt aatctcgtat aaggcattta
     1081 taaattcaac tttaaatgtc ccaggaagat gtccatttgg acgtttttct gccatttctc
     1141 cagcgatatt gtaaaccaac attgctgttt ctaatgattt caattcttga cctttttcta
     1201 gtccgataaa gcttgctact acagctccta ataagcatcc tgtcccaatg actttcggca
     1261 tcatagcact accattatga atcgttacca cttctccatt aacagcaatg gcatccactt
     1321 cacctgttac tactattgga atattgaact tctcatttgc tgctagagca atttcgtcaa
     1381 tattatctac gcccgcacta tctactcctt tagatgccac atctattcct actaaagagg
     1441 caatctcgcc agcatttcct ctaatcgctg ctagtttata attgttgatt agatcatctg
     1501 ctactttttt tctatattct cctgctccac aggctacagg atctaaaact gctgggacat
     1561 tatatttctc cgcaattttc agagcagctt ggtataattt ccaattttca tctgtcaatg
     1621 ttcctatgtt tattaataaa ccaccagcat actttaacaa atcctctaaa tctgctggaa
     1681 actcactcat ggctggtgag acgctcagtg ctactaatcc atttgctgtg aaatttttta
     1741 ctacatcatt ggtgatacaa atgaccaatg gtgctttttc ttttaataat tttaaacttg
     1801 tcatattgaa atccttcctt tacactttat acgatctact gatttcgatt tatctctatc
     1861 tttcgttaag aaattttttt catttacgtt gaatgattta tactcaatga aaatcaaaga
     1921 gcaaactagg aggctaaccg caggttgctc aaaacactac tttgagcctg tagataagac
     1981 tgacgaagtc agctcaaaac actgttttga ggttgtggat agaactgacg tggtttgaag
     2041 agattttcga agagtcttac ctcatcaaat ttgtaaatat catgagcctt ctctagacat
     2101 cgtaaccaat atcaaaaaaa gctaattcta aagcgactgc ttgattccag cgttgctgaa
     2161 gttctgtcaa atcttctcga tttttaccga cacgattgag ttcgtcaacc agaaattgaa
     2221 cccactctgc aaagaaagga cctctgtgga gattgatcca ttccgaatga atatagactt
     2281 caggtagagc caaatcttta gaaccccagt ctaaatagag accttctgca atgaccagca
     2341 taatcaaaag atgggcatag tctgatgaag ccactgccga atacattaga tcctgaaagg
     2401 cttttgttac agggtgcaaa gtcacttcta gatagtcatt ctctgctact tttaactctt
     2461 taaaagcctt ttggaaataa ccatcttcat ctgcttcaag aaagcctagt tgcttggcaa
     2521 aacgaagctt ggattcaagt ttatctgcgt gagctacgca ggcacccagc atggataaga
     2581 aggcatcaaa gaagtgataa tcttgaatca gatagtcctt taagacctta ttctcaattg
     2641 tccccgcaaa aagttcctta acaaaacgat gattgattgc agcctgccaa tccttctgac
     2701 tgctttttaa taattctcca acagtcaaac ctggctgaaa tgcatagtct tgtgtttcca
     2761 tatttacttc tcctctcttt acttgttagt aattaataaa acaccaagaa atatcaagca
     2821 aaatcgtaat tccacttgat ccttttaaag cacatcgaga gcatttgcag agagctaact
     2881 aaacaagcct atccagttta tataaacaaa aaactccaat tacaatcaag aattagagtt
     2941 gacttacaag attagatcgt tcatttcgtc atacgaaaaa acgtttcaca tttcccttcg
     3001 ccagtcttaa ctgtatcagg ttcaatgggt atcatctcag cttaaagcac cccaaatgtc
     3061 tctattattt aattactgaa ccagtatagc aaaaaatgaa agccctagca agatatttga
     3121 ccgaaaaata tctttat
//
LOCUS       DAVLSL010000171         3087 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLSL010000171 DAVLSL010000000
VERSION     DAVLSL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.173"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..175
                     /locus_tag="VXN30_001909"
     CDS             <1..175
                     /locus_tag="VXN30_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1358481.1"
                     /translation="NRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTL
                     ITGKNGTGKSTLL"
     gene            complement(541..1851)
                     /locus_tag="VXN30_001910"
     CDS             complement(541..1851)
                     /locus_tag="VXN30_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1358482.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2088..2960
                     /locus_tag="VXN30_001911"
     CDS             2088..2960
                     /locus_tag="VXN30_001911"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1358483.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          948 a          518 c          702 g          919 t
ORIGIN      
        1 aaacagaact gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat
       61 ttcgtatagg gtggatcatc gtatattgtt tgataatatt tcttttgata cttcgagttc
      121 aggcgtgaca ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtagat
      181 tgaaactaga atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactatcc
      241 tgatctattt gtcctgttct tatttcattt cactatatct caaattgagt atgacgaagt
      301 gcgctcccat gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga
      361 taaagactat tgggatgaat ttttaaagtt gaactaatca tttttacagg atgagattta
      421 cagcagagag tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt
      481 tccacttaat tttgtgtcta ctttattata ccaagtccac ccttaagtta gataataaat
      541 ctaacttaag gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct
      601 gatggggaca cccagaataa tttgcttttt gaaggcaagg ccacgttcct ctatattggg
      661 aagtgagagt tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc
      721 gccaataacg gtggctgttg tgagtaagtg aatatcaatc tgaggatttt gagcactgat
      781 gatagcaatg atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga
      841 catcactccg gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggttgttgt
      901 catgaggtgc cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct
      961 catgccgcag agcatgataa ttgtagccca gggaacctta gctaaaatgg cttcttgctt
     1021 ccctaatttg agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa
     1081 tgttttttga taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa
     1141 aagccaaacc aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct
     1201 gaggagtggt ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtgact
     1261 gtaacaggat aataaaagca agacgatgag tgggtagata atgctgacga taaagatatg
     1321 attgccaagt gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac
     1381 aatgcctgag ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg
     1441 agctccaatc aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta gaggacagca
     1501 aacggccata gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta
     1561 taaaatcatg tagagggcgt tagggtgggt gcgtgtgcgg tagagaatgt gttgagccaa
     1621 aacatcaaga gtaccgttag ttgttgcaac gttataaaag agagagacgc taaaaatggt
     1681 aaaaaagagt gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt
     1741 ggttgcgatg aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta
     1801 accaattcca atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc
     1861 ctttcagtta gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc
     1921 ctattggtac ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac
     1981 acttgttaga attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttaatagta
     2041 attgcctcta gtgcttacaa gtgatattaa aaatagagga cttagtgatg tcaatcattt
     2101 caactgattt aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg
     2161 ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa gccaaggtgt
     2221 cgctttttgc caaggaagct ggtgttttag cgggactaac cgtttttcaa agggttttta
     2281 ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt
     2341 tgactagtgg cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg
     2401 aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt
     2461 atgtagaagc cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta
     2521 atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat catcgcttta
     2581 atttatcaga tgctatcatg ctaaaagaca atcacattgc ggcagtaggt agtgttcaga
     2641 aggcaattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg
     2701 aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata
     2761 atatgtcatt ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg
     2821 aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt
     2881 acgtctccag tggtagttta acccatagtg ctaagagtct tgatttttcc atgaagggtt
     2941 taacctacct tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc
     3001 tagaacgagt tttatgtcag atagtttaaa cgcctcttca aatatagtaa aatgaaccaa
     3061 aaatagtaca caatgtggta taatctt
//
LOCUS       DAVLSL010000172         3010 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLSL010000172 DAVLSL010000000
VERSION     DAVLSL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.174"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(189..467)
                     /locus_tag="VXN30_001912"
     CDS             complement(189..467)
                     /locus_tag="VXN30_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358484.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            complement(452..691)
                     /locus_tag="VXN30_001913"
     CDS             complement(452..691)
                     /locus_tag="VXN30_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358485.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            complement(1824..2552)
                     /locus_tag="VXN30_001914"
     CDS             complement(1824..2552)
                     /locus_tag="VXN30_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358486.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSSWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            complement(2549..2902)
                     /locus_tag="VXN30_001915"
     CDS             complement(2549..2902)
                     /locus_tag="VXN30_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1358487.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
BASE COUNT         1065 a          568 c          389 g          988 t
ORIGIN      
        1 tacaaatttc tatatcataa gcttctactc caaaaaatag ataaagatac tacttttgcc
       61 cttttcgatt ctctttttaa acatagcatc atcttccttc attcctataa aaacatttcc
      121 gatgagagag cttgtttttt aatttcgtta aacaacaaaa aacgcaagct taagcctgcg
      181 gacaatcttt atagccattt catttctttc acatctgttt tacgaacttt agcatatttc
      241 ttagcgcttg taaaatgtag agtaactgat ttaaatactc cgcttttgtc tcttcgtaaa
      301 cctaaaactg cggatctttt atttttatcc tcaaaaaata tgacgtccaa tttcatagta
      361 tttttagata aatccctagc tacgatgcaa caacttatta ttttagaata gaaaatatca
      421 ccgataaact catataaaga tatcctttcg attacatcat tgtacatagg atatttctaa
      481 aatcttctaa aataaaaaca ccagcttgaa tagcaggttt tgtttgacta gcataatctc
      541 gtgtgatttt gtataagtca aataaatgat gtaaatcagt caagcaaaaa ttcactttga
      601 aaatctctaa atacttataa ttcgttgata cttgacatgt ttttccattg aatctttctt
      661 gatagtccaa aatacaatca agtaatttca acgacaaact ccatattttc tcaaaataaa
      721 aaagcacggg gcagttctca ctactgccca aagtggttga ttagagtcgg cttctctaac
      781 tccctatgtt gtgcttatta ggcttatcgc aagggaacag tttttagctc tgccaagcca
      841 catagtgtgc ttactactat gtgtccatgt caccatgaat tgccgaacgg tttaacgaga
      901 aagtatagta gattgaaaca agatgtgaac aaatcgatta ggaaagtcaa attaatttct
      961 agaaatattt taacagtcat aacggactat tccagattca atctactata ttacttttag
     1021 gtttatccca actcaattat gacatttttt tcaaaagtca atatatctca ctcttttcaa
     1081 cgacaagaaa gaggctgata atcgaccaac ctctgttttt agatacagtt caagtccaaa
     1141 ctattcttta tagaaaagtc cgataaattc gagcaatagt taacatactt acaaatataa
     1201 ctcccaaaat agataacact attttcacaa aactcagttc cgaacctatg acatatatat
     1261 gaaaaagaag ggctagtcct acacatatat acaataagta gccaacaagt tttatctcac
     1321 caattcctgc aaaaaaatgt ttcatgtgac gattctccat ttactccatt aaatcagttt
     1381 tattttagac tatcataaaa caatcacctc ttcaataaaa accatttgcg aaatactatt
     1441 ctgaagcatt gatagctcag atttctcaga atagagctgt cctaactatc gagcagctca
     1501 aatacatata tatgagcaat tctaagccta gaattgctat aaaataaatg tttccaggct
     1561 attctaagtc tttgactgct taaaatttta cattctgaag gattctaaag ctagaacagc
     1621 tcaaaatcac gagaactaag gtattcgaaa cttcagagta atacaatccc cataggaatt
     1681 ctccattctt attaagcatg attaaaatgt aatcctaaca agtgatcctc gactgtgttt
     1741 gtagcactta gttacctaga taagactatt tccctttatc atatctgatt ttaagccgtt
     1801 ctaagattta acctatgttt ctattacttg ctatttttaa ataattcgta ttcctttgct
     1861 ttgataaatg gaactgaaaa attagaaaaa agaagtaaaa ccgaaaacaa atagcctgct
     1921 ttttgaatca ctaaaagata tcctaaaaat aagtaagcta taacatccaa tgcgaaaaga
     1981 aaaaatattc tcctcttcat taaagtactt gtgtcaaata atctgatgaa taaaaatttt
     2041 atcgtagtat ctacacaaat ttttatcaaa aagataagag ccaatacacg attatcaact
     2101 gtaccgtaaa gaatagatgg aaatatagtt aatataaaac tagaaatctc taaatatttt
     2161 atccaagaag attgagacac cttgcttcta aactgactaa ttttcttata ttcgttagtc
     2221 aatttagtag caaaatcttc cccaattaaa tgaaagattg ctaactctag gataaaaaca
     2281 cttattaata cggtataagt caaaaaactt tctgggaaaa acttattgat taaaatgggg
     2341 aaactcatta acagaatcga atttatggct agacagatag atactatttt atttttcaca
     2401 gaatatagag gtagtgatac tattgagtag ataaaagcta aaaaaataag caaaaaatac
     2461 tccgtgtcat ttataatcgc cctgaatgat aaaaatatat acatccaaca tacgataatg
     2521 tgtatttttc gaatcttatc tacattactc atcttactgt cgcagatatg aatccatccc
     2581 aaggataagg gttcccgcta atatcacaac ctctattaag agcaccatat ccaatacagt
     2641 aagctattct tcctgccgcc ccagccaata cacctgctgc tgcaccaata agccaacctc
     2701 caaggcctcc aactttaata tagtttatta gttttttggt taaaactgca gctccagcta
     2761 atgctataaa agttcctgga ctcatagcta aagctgcgca aattccttga caagcacgct
     2821 tgcttaaata agctccccca tcaaggtaca tcatctcttc ttcatcaatc acaacataat
     2881 tatttggtaa tacaagttcc atttattttc tcctttttta ttaagcatgg ttaggatggt
     2941 ttcctaacaa gtgatcgcag gctttgttta gttacctaaa ttagacttcc tttctttatc
     3001 ttattttaaa
//
LOCUS       DAVLSL010000173         3001 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLSL010000173 DAVLSL010000000
VERSION     DAVLSL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.175"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(53..2836)
                     /locus_tag="VXN30_001916"
                     /pseudo
     CDS             complement(53..2836)
                     /locus_tag="VXN30_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT          972 a          686 c          446 g          897 t
ORIGIN      
        1 cagtctcact aaagaaatga aaatcataca tagaaatttt taaaaatttt ctctatcgta
       61 aacctgccac tagttcagaa actatactag ctgattccaa acgtaatatg gtcatctcat
      121 ctaccttccc taataatggc attgcaccat accaaacaat agtcttattc accaaaagaa
      181 taggcgttac tatttttgat tgagtaaaag acactttaat tttctggctc ttcaactggt
      241 ctagccatgc tttatttgca accgtctctg gtatacatat ctcaatttga ctaatcctaa
      301 attcttttag aaaattcatc agttttgtct ggtgcacata aggtaaaata agcagacatt
      361 cctgtctttc ccctgctaag tcctctctca acaccttctc atatctacta tcaacataaa
      421 cgaattgttt ctcctcaccc tcgatgacac gataatccat ctttcgataa gctacttgtc
      481 gtttctgaaa catcttttct aaataaggaa catgaatatc cacataatcg aaaatacgca
      541 ccaaagactt atccttgtag tttctatgaa tccgacctgc atactgaatt aaattatttt
      601 tccaagaaaa gggtgctgcc aagataagcg tgtccaactg aggtaagtca aaaccttcgc
      661 caatgtattt tccagtagac aacaaaacaa accctttatc taactgttct aacgtctcca
      721 gtaaactcgt tctttctcgg actttggttt ttccgctaat aatgtaacag ccatcaacct
      781 ctttctcttt caataacttt tcaaagacat ctatctgttg aattcgatta actaaaacca
      841 agatattccg tccttctgcc acttgggcta gaatatcctt gagaatcaac tgattcctcg
      901 ctgagtctgt agcaatccaa tcactgagct gtataaaatt acttgctttg gtcttttcaa
      961 tctccaaatg accaaaagaa gtgaatctta attgcaattg ccgtttaaaa tccgtttccc
     1021 tcttatcagc agtatggagt atctcaccaa ttctctgaaa aacaataggc tcatgaccat
     1081 tcttacgctc aggcgtagcc gtcaaaccgt aaagatactt ccctctaaac tgagcaacaa
     1141 ctttttcaaa catcaaggca gagacatgat gacactcatc cacaatcatc atctcatact
     1201 catccaaaag actttgacta ttttccaact taaatagaga ttgaatcata acgacatcaa
     1261 ccagtttact cagccatttc ttagtcccac cgtactgtcc aacatagccg attacctttt
     1321 cacgacctga tgccgtataa cggatagcct cctcctcttc gaaagtcaaa aagcagttta
     1381 agcgatctag ccattggtct aagagttgct tattatggac tagaataatt gtttttgttt
     1441 tccgttcaga gataagagca gcacctaaaa cggtcttccc aaaaccagtc tccgcatgaa
     1501 gtaaaccatt ttctttagaa ttcatatctg acagggctaa ttcttgctcc aaagtaagct
     1561 ctcccttaaa tgctactcta atagaccttt gtacttttct cctatcctcc acaactacct
     1621 gcttaaattt atcttgcaat ggatataaca agcctcttgg cagccataaa taataatcgg
     1681 attctccaaa taaatacatt cgctcaggaa tttgataggt tggctgtcgc atagcctgct
     1741 ttaaatatag taaaatgaaa taataacagg acaaatcgat caggaaagtc aaatcgattt
     1801 ctaacaatgt tttagaagca gaggtgtact attctagttt caatccacta taaaattcgg
     1861 gattagaaaa ggaagccata tttttcaaga aaaaaagtgc cttggatgtc acagacgatt
     1921 tttcaagttg gatcatattg gataaaacga ccttcaactc cttatccaat tcttgcttgc
     1981 ctaactcctc ttggattaac agtgccactt tagcagttga aatcctctga atttcttgta
     2041 gatacctcca ttgatcttca taaggctgaa aatgttcatc cacaaagacc gttcgccctt
     2101 gatgataagc ttctccttga aaaggaaggg aaattaaatt atcaaatcca cctttaggaa
     2161 gaacatcctg atttggaaac atgccatcaa aagaatcaaa ggacagttgc atactttcct
     2221 gcatagccaa ttctaatagt ttctttccaa acaggcgagc ctttcgactc agaatcgctt
     2281 cctcaaagaa gaaccaaata tggagtccgt aacccgaacg agaaatctct aaatgggctt
     2341 ccatctggcg ttccctggct attcttcgaa tggttaaacc agcttctttc caatctcctt
     2401 catcaaaatc cagtaccaaa aaatgacagc tatcatctaa gtgcatagga aagataccga
     2461 tagctgcctc cccacgaaaa tgagatttca aaacggagtt cgtcaatgtc tggaaactcc
     2521 gtttttcagg tggcaactgc ttccaaccat aatcatagga aggaaaatac tgaattttcc
     2581 cttgttcatt gataaaattc ttagcataaa catcataacg tccagcaaac acggaggtaa
     2641 acaattgtaa tttttcttgc gtcgtcaagc gactactttg aagttggaac atctcctcga
     2701 actgagagcg ttccataaca aactgactct tgtctttaag acgtttacac aagaaaaact
     2761 cactatcccc attaaaagaa gaaaagacat caagtacttc taccacaatt ccatccaaag
     2821 acacaaaaac agccataaga gtcacctcct tgattcctat aggctgatta taacaagaat
     2881 agctgaaatt gtacacgaaa ataaaatcct aatagtactc attttgtatg tggctaatat
     2941 tccgtctcgc tccggaatgt acgaggtaaa tagagtttaa acgagcagtc tataaaattc
     3001 a
//
LOCUS       DAVLSL010000174         2938 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLSL010000174 DAVLSL010000000
VERSION     DAVLSL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.176"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            27..167
                     /locus_tag="VXN30_001917"
     CDS             27..167
                     /locus_tag="VXN30_001917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358488.1"
                     /translation="MADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFDMAKTQIE
                     TD"
     gene            259..1938
                     /locus_tag="VXN30_001918"
     CDS             259..1938
                     /locus_tag="VXN30_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1358489.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            2206..2772
                     /locus_tag="VXN30_001919"
     CDS             2206..2772
                     /locus_tag="VXN30_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358490.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1110 a          370 c          636 g          822 t
ORIGIN      
        1 atatattgga caatgactgg aatggattgg ctgatttaat agcaaatctg attgcaaaat
       61 atgctggtgc tttagattta gataatctac ccgatccaac gccagcaaaa aatcaagaga
      121 tgaaaaatag ttttgacatg gcaaaaacac aaattgagac ggactaataa aagtgttata
      181 atatacttga aatgatagtc caaacttaaa ttaaaaaaat tatatatgat atagaaaaat
      241 tatattgagg ttaatactat gtcaaaagaa aaaataaaag tatacctcta tacacgagta
      301 tctacgtcaa tacagataga ggggtattcg ttagaggcac aaaaatcacg aatgaaagct
      361 tttgctattt acaacgatta tgaaattgtt ggagagtatg aagatgccgg gaagtctggc
      421 aagtctattg aaggtagaat acagttcaat cgaatgatgg aagatataaa atctggaaaa
      481 gatggagtat cttttgttct tgtgtttaag ttatcaagat ttgcaagaaa tgccgctgat
      541 gttctttcta cacttcagat aatgcaggat tatggagtta atcttatctg cgttgaagat
      601 ggcattgatt catctaaaga tgctgggaaa ctaatgattt ctgttttatc agctgtggct
      661 gaaatagaaa gagaaaacat ccgtattcaa acaatggaag gttgcattca aaaagcaagg
      721 gaaggaaaat ggaatggtgg ctttgctccg tatggatata aacttgaaga cggcaagctg
      781 tttataaatg aggaagaggc agttgcaata agaacgattt tcgaccagta tgtaaatact
      841 acgataggag ccaatgggat atctaaatac ttagagaatc atggaattag aaaaatccca
      901 agacagaatg gtaagaatcc tttgtttgat gcaggtctta taagaaagat attaaagaat
      961 cctgtatata atgggaaaat agcatttgga agaagaactt tagaaaaagt tcatggtaca
     1021 agaaatgaat ataagcaggt tgaacaagat gaatatctaa tatctgaagg gatacatgaa
     1081 gctatagttt ccgatgaagt ttggcaagct gctcaggtta agctaaaatc tcaagcaaag
     1141 aaatatgagc atgtgaataa aggaaaagat acacgcacac acttgctttc tggaattgta
     1201 aaatgcccga tatgtggagt gggaatgttt gggaacaagt gtatcaagaa aaagaaagat
     1261 ggcacaaagt ataaagattt ttattactat ggttgtaaac ataggcagat gataagaggt
     1321 cataagtgta cattcagtaa gcaaattaga gaagaattgt tagatgatgc tgttgcagag
     1381 gtgattgtca agatagtaag taatccgaaa tttgcttcta tgatgcaaga aaaaatcaac
     1441 atgaaggtgg atacctctga aatagaaaaa gaaatagata attaccaaaa ggaattgaga
     1501 aagagtcatt ctacaaagtt taagctaatt gaggaaatag ataatttaga tgttgaagat
     1561 aagcactata agagaaggaa acaggattta gatgatagac tatatcgtat gtatgacaaa
     1621 atagatgaat tagaatcatc actaattgat gcgaaagcaa agaaacagac tattgaagct
     1681 gaaaaactta caggagataa catatataag gttctgatct attttgataa gctctataaa
     1741 gtcatgaatg atgtagagcg taggcagtta atttcagctt tgatttctga aattcaagtt
     1801 tatgaagaaa aacaatctaa cggacaatgg ctaaaatcca ttacttttaa gctaccaatt
     1861 atagaagaaa atctcaacat agatttggac aatgatgaac aagttgaatg tgtaagttta
     1921 ttagagaaac gtagttaatc ctcaaaaggt tccccaaact ttttgagtcc cactgacgca
     1981 tcacgctgag gcggtatcac tgcttgtacg agttgaagca tcagcgaagt aaaagtagat
     2041 gttcagccaa tgcgacgagg tgagaccgaa ggtcgataag gtgtgttctg ggtataggac
     2101 tcgtaaagta agtaggaaat atgatgagat gatacagaga gcctgaattg gtcaggtgac
     2161 gtgcttattt acattagcct gataatgaaa atggagagat tatcgatggt tatattggaa
     2221 ttgtaccaaa atgattattc taaagattta gtagcatttg attccataga agatgggaaa
     2281 gcctttgtag ctcaaatccc gggatacact ctggaaacag aagatggttt tgaagtggag
     2341 tattttaatc ctaaaaacat accggattat atggaaatta tttttaacgg aaatattgtt
     2401 cctttatcta aatttatgtt cgatcctgag gaaaatgtaa acattatttg gaaagagatt
     2461 tcaaatttat ccctcaaaaa tgatagagtg atcgaaggat attcaaaaat tgatgcatat
     2521 gtcgttaaca accatgaagt taaagcctat gtcgaaacaa gagaaacgaa ctatcgtaaa
     2581 gcaaaagact tcttagaaag ccgcggatat gaaatcgaca gaagcttttt cggaagtgaa
     2641 gacggtgaag cgattcttta cagaaaaaaa gggatcgaag tttggcattt cttgtgtcac
     2701 ttagacccaa tgtttgtaga gattgaagat gtagaaggat atgttaaaga agaagttgga
     2761 gagattcaat agtcaaccaa agttccttgt gagtatatac ctttgctagt aaaagagtca
     2821 aaacgttata acgaaggggg gaagaaaacc cttgagtgta agtctttttc ttgtttagag
     2881 ggaaagtatt agagtagccg ctcataattt atactagatt tgataactga tttgagac
//
LOCUS       DAVLSL010000175         2898 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLSL010000175 DAVLSL010000000
VERSION     DAVLSL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.177"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(109..642)
                     /locus_tag="VXN30_001920"
     CDS             complement(109..642)
                     /locus_tag="VXN30_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1358491.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(731..1507)
                     /gene="recX"
                     /locus_tag="VXN30_001921"
     CDS             complement(731..1507)
                     /gene="recX"
                     /locus_tag="VXN30_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1358492.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1545..>2898
                     /gene="rlmD"
                     /locus_tag="VXN30_001922"
     CDS             1545..>2898
                     /gene="rlmD"
                     /locus_tag="VXN30_001922"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1358493.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          911 a          524 c          533 g          930 t
ORIGIN      
        1 tgtgttgtaa ctctgctata atggatttat tcctttttgt atttacacaa tttattttat
       61 actaccaaaa aaacaaggtc aggattttgt tcctgacctt tgacaacttt accgattctt
      121 tagttctaca tagcgcttgt accaaatgtt tacataggct tctgagaaag gaccacgtcc
      181 attgttaatc caatcaacaa gaattttgac atgttctttt aaaatatagt ccaagtcatc
      241 agaataattc attttgcgtt tgtgacgctc gtattcttca acgtccaaga gacgtttttc
      301 cccatctgta aaaattttaa catccaaatc gtaatcaata tacttcagtg cttcttcatc
      361 cagatagtag gggctagcca tattgcaata gtaagaagtt ccattatcac gaatcatggc
      421 aatgatatta aaccaatatt tcttgtgaaa gtaaacaata gccggttctc gagtgaccca
      481 acgacgacca tcactttcgg taacaagtgt atgatcgttg acaccaataa tggcgttttc
      541 tgttgttttt agtaccatgg tgtcccgcca agttcggtgg agactcccat catgcttata
      601 actttgaatt gtaataaagt cgccttcttt tggaagcttc ataactaacc aactttctac
      661 aatttataag tttatcattt actattgtac cataaaatta cctaaaatct gtgaatttca
      721 cttggaaata ttaaagatat tctctaagag cgcttgctat atccgaaaaa tcgtagccct
      781 ttcgtgctaa aacttgagtt aaacgctgct tcagttcgta tccttcatac tttcgagcat
      841 acttagtata ttgcttatca agttccttga agatgagttc ttgagtcgtt tcttggtcaa
      901 cttggctatc taagtcgtca aaggcaattt tagcatcaga ataagagaag cccttgttag
      961 tcaagttctg gataatctta tcttgcaggg cacgagctgg aagttttccc tcatattttt
     1021 tcaatagttt attggctaca cgttgagcaa cttcagaaaa atcaaattct ttcaagttct
     1081 cttctatagt agattttgaa atcccttttt gagatagttt ctgagtcagc acataaggac
     1141 ccttgtctcc tgaaagttga ttggtattga tgatagcata agcgtactga ccatcattaa
     1201 tccacttatc ttctttaaga ttagcaataa cttgagaaac gatgttttta tcaatatcgt
     1261 attttttcag atattctctg acttcttttt cagtgcgtgc tttaaaggat aagtggtaga
     1321 gagcgagatt cttaccataa gaaaattgag caaagtcttg aatctctttc aattcctctt
     1381 cgcttatcac cttatctctc gataacataa aacgaacaat tgtatcttcg gtgatatagc
     1441 atttgtcgcc attatcaagc tccatcaggt agagtctttt tttcttttca agttttgtga
     1501 ttttcatagc tctattataa ctcaaaatgt gataagatag gggtatgaat ctgaaagtga
     1561 aacaaaaaat accattaaaa atcaagcgca tgggaattaa cggtgaggga atcggctttt
     1621 accaaaaaac attagtcttt gtaccaggag ctctcaaagg cgaagatatc tattgtcaga
     1681 ttacttctat tagacgcaac tttgttgaag caaaattact gaaggtcaac aagaagtcta
     1741 aatttcgaat tgtgccatct tgtactattt ataatgaatg cggaggctgc caaatcatgc
     1801 acctgcatta tgataagcag ctggagttca agacggactt acttcatcaa gcgctgaaaa
     1861 aatttgctcc tgcaggatat gaaaattatg aaattcgtcc aactattgga atgcaggaac
     1921 caaaatatta cagagctaag ttacaatttc agactcgaaa atttaaaaat caggtcaagg
     1981 cgggcttata tgcacaaaac tctcactatt tagtagagtt gaaagactgc ctggtacaag
     2041 ataaggaaac ccaagtgatt gctaatcgct tagcagaatt acttacttat caccagattc
     2101 caatcacgga tgagagaaaa gttctaggtg tccgtactat tatggtccga cgcgcgagaa
     2161 agaccggaca ggttcagatt attattgtta caaaccgcca gcttaattta actcaattgg
     2221 taaaagagtt ggttaaagat ttcccagaag ttgtgacagt agctgttaat acaaatacag
     2281 ctaaaaccag tgagatatat ggtgaaaaga cagagattat ctgggggcaa gagagtattc
     2341 aagaaggtgt actcaattat gaattttcac tatcccctcg agctttttat caactaaatc
     2401 ctgagcaaac agaagtcctc tatagcgaag cagtaaaagc gctggatgtt gataaagaag
     2461 accatttgat tgacgcttat tgtggagttg gaacgattgg atttgccttt gcaaagaaag
     2521 ttaaaacact cagaggtatg gatattattc cagaagctat tgaagatgcc aagcgaaatg
     2581 ctaaaagaat gggatttgac aatactcatt atgaagctgg aacggcagaa gagattattc
     2641 ctcgttggta caaggaaggc taccgagcag atgctttgat tgttgaccca ccacgtacag
     2701 gtctggatga taagttatta gatactattc ttacttatgt accagaaaaa atggtttata
     2761 tttcttgtaa tgtttcgacc ttggctcgtg atttggtacg cttagtagaa gtctatgatc
     2821 ttcattatat ccagtcggtc gatatgttcc cacatacagc tcgaactgaa gctgttgtaa
     2881 aattaataaa aaaagttt
//
LOCUS       DAVLSL010000176         2887 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLSL010000176 DAVLSL010000000
VERSION     DAVLSL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.178"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..229
                     /locus_tag="VXN30_001923"
     CDS             <1..229
                     /locus_tag="VXN30_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEV1358494.1"
                     /translation="EQSGCLDMVVIQKQNEIEELSINMEKKIMNYIFEDDGNVSEVIS
                     ALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            242..1717
                     /gene="galT"
                     /locus_tag="VXN30_001924"
     CDS             242..1717
                     /gene="galT"
                     /locus_tag="VXN30_001924"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1358495.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1730..2740
                     /gene="galE"
                     /locus_tag="VXN30_001925"
     CDS             1730..2740
                     /gene="galE"
                     /locus_tag="VXN30_001925"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1358496.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
BASE COUNT         1043 a          383 c          597 g          864 t
ORIGIN      
        1 ggaacaatct ggatgtttag atatggttgt tatacaaaaa cagaatgaga tagaagagct
       61 tagtataaat atggaaaaga aaataatgaa ttacatattt gaagatgatg ggaacgtttc
      121 tgaagtaatt agtgctttag atattattca tcaccttgat aaaattgctc atactacgca
      181 agcaatttat aaatggataa tgtatagaaa atatgggaac ataaattaga aagaaaaata
      241 tatgaaaaca attattgata attttgttga tagagtaatt gaatacggaa tgtataatga
      301 aattgacaaa atatatgtga aaaatagagt actagcgtta ataggtgagg agggaattga
      361 tcgcatttcc gatgaaaatg acctgaaaca aataaaagat tacttagtgg aaatcgcctt
      421 aaaaaacggt aaaattaaag atttaatcga ggaaaaggaa tgtttaggag cagagttaat
      481 gaatttcata gttcctttac ctagtcgatt aaatgatatt ttttggagtt catatgatat
      541 atcacctcaa gaagcagttg aagagtttta taagttaagt aaagatagtg attatattaa
      601 aacttctgct attgctaaga atattgagtt tcgagcatca actaaatacg gagagctaga
      661 gattacgata aatttgtcaa aacccgaaaa agatccaaaa acaattgctg ctgaaaaatt
      721 agtaaaagca actaattatc ccaaatgttt actatgtatg gaaaatgaag gatatcaggg
      781 gcgaataaat tatccagcac gttcgaatca tagaattatt cggttgaaac ttggtgacga
      841 agtatgggga ttccagtatt ctccctactc atactttaat gagcatgcga tatttttgaa
      901 tagtcaacat gtaccaatgg ctattacctc taaaacattt gaacaattat tggagattgt
      961 tgatattttg ccaggatatt ttgctggttc aaattctgat cttcctatct caggaggctc
     1021 cattcttagt cataaccact accagggagg gaaacatatt ttcccaatgg aaaaagcaaa
     1081 atttgagagt gaattttgtt ttaaagactt tgaggatgtt aatgctggta ttgtaaaatg
     1141 gccaatgtca gtaattaggt tacaaagtga aaataaaaat cgcttgttag atttagctac
     1201 taaaattcta aataagtgga gagaatattc agatttagaa gtagacatca ttgctatgac
     1261 tgaagatgtc ccacaccata cggtgactcc tattgctcgt aaagtggacg ggagatacga
     1321 attagatatt gttctaagag ataatcatac tactgaacaa tacccagatg gtgttttcca
     1381 tcctcatcaa gatgtacaac atattaaaaa ggaaaatatc gggttaatcg aagtgatggg
     1441 acttgcgata ttaccacctc gtttgaaacc agagttagaa gaagttggaa aatatctttt
     1501 aggagaggat aatgcaattg cggattatca tttagaatgg gccgaccaat tgaaagaaaa
     1561 atatcctcgc attaataaag aagaggttaa tagcgtagtt caacacgaag caggacaagt
     1621 gtttgcgaga gttctggaag atgcaggagt ttataagaat acaccgtctg ggcatgaagc
     1681 gtttatgaga tttgttaagt cggttggaat taattaggag gaaacccaga tggcaatatt
     1741 ggtaacaggc ggagctggtt atattggtag ccataccgta gtagaattac taaatttagg
     1801 aaaggaagtt atcattgtcg acaacctttc gaactctagc aaattggtgc tagaccgtat
     1861 tgaagcaatt acaggaatac gtcccgtgtt ttacgaatta gatgtttgtg ataaacaagc
     1921 attgagaaag gtatttgaac aagaatcgat tgatgctgca attcattttg caggttataa
     1981 agctgtcgga gaatccgtgc aaaagcctgt gatgtactac aaaaataata ttatgagtac
     2041 attggcactt gttgaagtga tgtcagaatt taatgttaaa aagattgtct tttcttcaag
     2101 tgcgactgta tatggaatta acaatcagtc acctctaatt gagacgatgc aaacaagtgc
     2161 gacaaatcct tatgggtata cgaaagtgat gcttgagcaa attttaaaag atgttcatgt
     2221 ggcagattca gaatggagta ttgcgttgct tcgttatttc aatccaattg gtgctcatga
     2281 gtctggtttg attggggaag atccctcagg aattcctaac aacttgatgc cttatattgc
     2341 acaagtagcg gtaggtaagc tatcagagct aagtgtgttc ggaaatgatt atgatacgct
     2401 tgatggtact ggtgtgcgtg attatatcca tgtagtagat ttagcaatag gacatataaa
     2461 agctttagaa aaagtatctg aaaaaacaga tgtttatatc tataacctag gctcgggaga
     2521 aggcactagt gtattacaac ttgtaaatac atttgaaagt gttaataaga tccctattcc
     2581 ttataaaata gttccaagac gttcaggaga cgttgcaact tgttatgcaa atgcagacaa
     2641 agcatataag gaattaaatt ggaggacaac aaaatcgatt gaagacatgt gtagagatac
     2701 atggaattgg caatcaaaaa atcccaatgg ctataattga tgatactgtc tatatgagtg
     2761 ctgtattttg aaaaaattac ttaacctttt aatgaaatgc atatttaatg aaagataaat
     2821 ttaaaaaaat gtttgcaagt gcttgacaaa ggaaacggtt tcattgtata atgtaaacat
     2881 gatgaca
//
LOCUS       DAVLSL010000177         2873 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLSL010000177 DAVLSL010000000
VERSION     DAVLSL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.179"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(104..1342)
                     /locus_tag="VXN30_001926"
     CDS             complement(104..1342)
                     /locus_tag="VXN30_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1358497.1"
                     /translation="MRITDNQHKIAKEDFVAEYPKLSQALLDRTLDNLSREDNIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIECIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRMNKIKLIRHAYFREYRKLQELCLVILSREKHGLG
                     PQAQRVHGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEIGNL
                     AGYGLFESLRMPHSIVHFVK"
     gene            complement(1329..>2873)
                     /locus_tag="VXN30_001927"
     CDS             complement(1329..>2873)
                     /locus_tag="VXN30_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1358498.1"
                     /translation="DLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTKLLCIYTE
                     GDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKLGTMLFEY
                     SKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEIAKELTDG
                     NEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKETQLIGGQ
                     DNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLPSKYVYEL
                     YKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEINRGEISK
                     IFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDRSVDTFDF
                     AMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSHYHIGPSY
                     FLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQTGQQDTG
                     DNDADN"
BASE COUNT          896 a          557 c          450 g          970 t
ORIGIN      
        1 cgttcgggtt ttacttgtgt taaaaataaa agaaagaggg gaaattattt tctccccact
       61 tcttcatcaa taatcgcttt aaaattttct tctgaatttt ccatcatttt acaaaatgta
      121 ctatagaatg aggcattctg agggattcga atagaccata acctgctagg tttcctatct
      181 cattatcaat aaccttgtcc ttgctcggaa aaacaagtcc agccttctca gcttttaaaa
      241 tataagaata ggaaatcagc tggaataagt cctcacggtt gattcctttt tcagtcaatt
      301 ccagtttttt atattttgca tctagaacaa tctttcgttc tctatcataa aaatctggat
      361 atacttttcg tttcccaaca gaaaatactg aaattccatc cgtcttatcc ttatttctgg
      421 gatgtacaaa accttttggc aacaaggtgt aaacatactc ttcccaaagc caggcaacat
      481 caaagagaat accatgtacc ctttgggctt gaggtccaag accatgcttt tctctactca
      541 gaatcaccaa gcagagttcc tgtaattttc tatactctct gaaataggcg tgtcggataa
      601 gtttgatttt attcattctg ataatcttag cacgatcagc tagtttataa gagggcgtta
      661 cacgcacgat ttcagatacg ttttcacgac tagttgagag attatctagt accccttgac
      721 caatgctttt ctgattctta atgcattcaa tagtgtgacg gaccaactgc atgaggggat
      781 tatcataggt gaactctctc gttgtgtagg caatatttcc cgtgaaagga agatttttct
      841 tgagatggtt tcttacatca attactccct taacatgact gtcgttatga gaaaatcgat
      901 gatattcctt ataaagacct tttcgaatag cagcttgtag atacttgggg aagagataca
      961 tcaaaagttg ataaagcctc tcttcacgag acaaagcaac atctaaacta gtgagattga
     1021 tatgaagaac cttgtttaag agataatgca aaaaatggtc attactctca tcagaaaatc
     1081 gtgaggaaat cgttaatctt tcctgaccat atccaagaaa accaatcacg ttccctgtct
     1141 tgattttctg attgactgtt tcaaaaatct tttggtcctt atccaaatca ggagtatgag
     1201 tcaaatcatt tggaaaaata aagatattat cctctctaga aaggttatcc agtgttctat
     1261 caagaagagc ttgacttagt ttgggatatt ctgcgacaaa gtcttcttta gcaattttat
     1321 gctgattatc agttatccgc atcattatca ccagtatctt gttgaccagt ttgatcatta
     1381 tttgtcagtt caaatgcttt tttcaaagtt tccaaagttt caacctcatc ataagaacct
     1441 cgcaagtagt cttctaggag aggcttaata taatcagacc agagtaattc atagtcaaaa
     1501 tctacatcct tcaacttaag aaaataactt ggtccaatat gataatgact gtttaattcc
     1561 tgaacatttt cgatagcagc gttcaagttt cttagacgaa tttttgcttc ttctgcatgg
     1621 atattcagct ctttatccaa catgccaact tgaccctcgg cagtaacttc aacaaaacga
     1681 aaacgacgac gcatagcaaa atcaaaggta tccactgaac gatcaatatc attcatagtt
     1741 ccgatgatgt aaacattttc ggggatatag aacttttcat cagtttcgtg tagatttgca
     1801 tattgggtag aaacacttcc tttttcacca cgatagccag ggtcgataga aaaaaagagt
     1861 tcgccaaaaa tcttagaaat ctccccacga ttgatctcat cgatgatgaa gacgaatttc
     1921 ttatcagtat caatttctgt tggggaaaca tagtctttca aaccaaatct ctttctcaat
     1981 gtttctagga cagtttttcc accacttttg tagtattctt gcttattata atttttatct
     2041 ttatacaact cgtaaacata tttgctaggt agtgaccatc ctggaacacc actatcataa
     2101 tttactgaca aattttgtct actattaaca gataagtaag atgtttttgt tatatattct
     2161 ttttcttcag caacatttat atattctaag taagaatccc aagcctcatc aaaattatct
     2221 tgtcctccaa tcaattgggt ttcttttgct ttctgacaaa aatctttaaa aataccgtcc
     2281 tgtagcctaa actcaatagc tccatcccca tttgatactg gtcttaaacc ttctacaaaa
     2341 tccgtataat catatgatgg gtgaaattgt acaaatccga tttggtcttc gttgccatcc
     2401 gttaattctt tggcaatttc tttagcaaga taagtctttc ctgtgccagg agcaccacga
     2461 aggataaggt ttggagactt taatagagat tgagttaatt gatgactaaa attgtttgat
     2521 gaatccattg tactattgtc ttccttattt tccataaaaa gcttagaata ctcaaaaagc
     2581 atagttccta atttcgatgg ctccaactca ggataagttt tggacaccag ttcaattatt
     2641 ttatgatttt gcagataagg tccaccttga tttgtctgag aaacaaagtg gttccaaaat
     2701 tctttctgac tattaatatt tacaccaaag aaaggatctc cctcagtata gatacaaagt
     2761 aattttgtca ccatagcaga acgaccaata aattcatttg ttgatctttt catatcaaaa
     2821 ataggatttt caaagttaaa acgaatacct tctttgataa tttcatacaa atc
//
LOCUS       DAVLSL010000178         2824 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLSL010000178 DAVLSL010000000
VERSION     DAVLSL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.180"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..1218
                     /locus_tag="VXN30_001928"
                     /pseudo
     CDS             1..1218
                     /locus_tag="VXN30_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1322..1409)
                     /locus_tag="VXN30_001929"
     tRNA            complement(1322..1409)
                     /locus_tag="VXN30_001929"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1373..1375),aa:Ser,seq:gct)
     gene            complement(1416..1489)
                     /locus_tag="VXN30_001930"
     tRNA            complement(1416..1489)
                     /locus_tag="VXN30_001930"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:Ile,seq:gat)
     gene            complement(1525..1595)
                     /locus_tag="VXN30_001931"
     tRNA            complement(1525..1595)
                     /locus_tag="VXN30_001931"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1561..1563),aa:Gly,seq:tcc)
     gene            complement(1615..1687)
                     /locus_tag="VXN30_001932"
     tRNA            complement(1615..1687)
                     /locus_tag="VXN30_001932"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1652..1654),aa:Phe,seq:gaa)
     gene            complement(1691..1764)
                     /locus_tag="VXN30_001933"
     tRNA            complement(1691..1764)
                     /locus_tag="VXN30_001933"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1728..1730),aa:Met,seq:cat)
     gene            complement(1775..1864)
                     /locus_tag="VXN30_001934"
     tRNA            complement(1775..1864)
                     /locus_tag="VXN30_001934"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1826..1828),aa:Ser,seq:tga)
     gene            complement(1875..1948)
                     /locus_tag="VXN30_001935"
     tRNA            complement(1875..1948)
                     /locus_tag="VXN30_001935"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1912..1914),aa:Ile,seq:cat)
     gene            complement(1962..2035)
                     /locus_tag="VXN30_001936"
     tRNA            complement(1962..2035)
                     /locus_tag="VXN30_001936"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1999..2001),aa:Met,seq:cat)
     gene            complement(2041..2114)
                     /locus_tag="VXN30_001937"
     tRNA            complement(2041..2114)
                     /locus_tag="VXN30_001937"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2078..2080),aa:Pro,seq:tgg)
     gene            complement(2130..2203)
                     /locus_tag="VXN30_001938"
     tRNA            complement(2130..2203)
                     /locus_tag="VXN30_001938"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2167..2169),aa:Arg,seq:acg)
     gene            complement(2213..2298)
                     /locus_tag="VXN30_001939"
     tRNA            complement(2213..2298)
                     /locus_tag="VXN30_001939"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2262..2264),aa:Leu,seq:taa)
     gene            complement(2306..2377)
                     /locus_tag="VXN30_001940"
     tRNA            complement(2306..2377)
                     /locus_tag="VXN30_001940"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2343..2345),aa:Gly,seq:gcc)
     gene            complement(2400..2472)
                     /locus_tag="VXN30_001941"
     tRNA            complement(2400..2472)
                     /locus_tag="VXN30_001941"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2437..2439),aa:Thr,seq:tgt)
     gene            complement(2491..2572)
                     /locus_tag="VXN30_001942"
     tRNA            complement(2491..2572)
                     /locus_tag="VXN30_001942"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2536..2538),aa:Leu,seq:tag)
     gene            complement(2578..2650)
                     /locus_tag="VXN30_001943"
     tRNA            complement(2578..2650)
                     /locus_tag="VXN30_001943"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2615..2617),aa:Lys,seq:ttt)
     gene            complement(2675..2747)
                     /locus_tag="VXN30_001944"
     tRNA            complement(2675..2747)
                     /locus_tag="VXN30_001944"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2712..2714),aa:Asp,seq:gtc)
     gene            complement(2750..2822)
                     /locus_tag="VXN30_001945"
     tRNA            complement(2750..2822)
                     /locus_tag="VXN30_001945"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2787..2789),aa:Val,seq:tac)
BASE COUNT          821 a          723 c          550 g          730 t
ORIGIN      
        1 agaaatcatc gccaaactgg actacgacgc cccatcttgc cctgagtgcg gaaaccaatt
       61 gaagaaatat gactttcaaa aaccttctaa aattccttat cttgaaacga ctggtatgcc
      121 tactagaatt ctccttagaa aacgccgttt caagtgctat cactgttcaa aaatgatggt
      181 cgctgaaact tctatcgtca agaagaatca ccaaatccct cgtatcatca accaaaaaat
      241 tgcgcaaaag ttgattgaga agatttctat gaccgatatt gctcatcagc tggccatttc
      301 aacttcaact gttattcgta agctcaatga ttttcacttt aaacatgatt tttcttgtct
      361 tcctgagatt atgtcttggg atgagtatgc ttttacaaag ggaaagatga gcttcattgc
      421 gcaagatttt aacaatctca atatcatcac tgttcttgag ggtagaacac aagctatcat
      481 tcgaaatcac tttcttcact acgatagagc tgtccgatgt cgcgtcaaaa tcattactat
      541 ggatatgttt agcccttact atgacttggc taaacagctt cgctttcaaa tttctaggct
      601 caggctgaaa cagtctccca gactgtttca ctcccgaatg ctaaaatcgt tcttgatcgc
      661 tttcacattg tacaacatct tagccgtgct atgagtcgtg tgcgtgttca aatcatgaat
      721 cagtttcatc gaaaatccca tgaatacaag gctatcaagc gctactggaa actcattcaa
      781 caggatagtc gtaaactcag cgataaacat ttttatcgcc ctacttttcg tatgcattta
      841 accaataaag agattttaga caagcttttg agctattcac aagacttgaa acaccactat
      901 cagctctatc aactcttgct gtttcacttt cagaataagg aaccagagaa atttttcgga
      961 cttattgagg acaatcttaa gcaggttcat cctatttttc agactgtctt taaaaccttc
     1021 ctcaaagata aagaaaagat tgtcaacgcc cttcaactac actattctaa tgccaaactg
     1081 gaagcgacca ataatctcat caaacttatc aagcgcaatg cctttggttt tcggaacttt
     1141 gaaaacttca aaaaacggat tttcatcgct ctgaacatca aaaaagaaag gacgaaattt
     1201 gtcctttctc gagcttagct tttcttcaac ccactacagt tgacaaagag ccttaagttt
     1261 acggatctga agttttattg tctctaaaaa caaaaaagag agttacaaac tctcattaaa
     1321 acggagaata agggattcga acccttgcgc cagttacccg acctaacgat ttagcaaacc
     1381 gtcctcttca gcctcttgag taattctcca attaatgggc acgagtggac tcgaaccacc
     1441 gacctcacgc ttatcaggcg tgcgctctaa ccacctgagc tacgcgccca agttaaaaaa
     1501 cttggtaatt tgaacaaagt tcaaagcggg tgacgagaat cgaactcgcg acaacagctt
     1561 ggaaggctgt agttttacca ctaaactaca cccgcataaa tactatcaat aaaatggcgc
     1621 gagacggaat cgaaccgccg acacatggag cttcaatcca ttgctctacc aactgagcta
     1681 ccgagcctta ttgcgggagc aggatttgaa cctacgacct tcgggttatg agcccgacga
     1741 gctaccgagc tgctccatcc cgcgttaata taaaaggagg atgtgggatt cgaacccacg
     1801 cacgctttta cacgcctgac ggttttcaag accgttccct tcagccggac ttgggtaatc
     1861 ctccaatatt caaatggacc ttgtaggact tgaacctacg accactcggt tatgagccga
     1921 gagctctaac cagctgagct aaaggtccga caagatcatt atagcggcga aggggatcga
     1981 acccccgacc tcccgggtat gaaccggacg ctctagccag ctgagctaca ccgccatgaa
     2041 tcgggaagac aggattcgaa cctgcgacac cttggtccca aaccaagtac tctaccaagc
     2101 tgagctactt cccgagttaa atagaaaaat gcaccctaga ggagtcgaac ctctaaccgc
     2161 ctgattcgta gtcaggtact ctatccagtt gagctaaggg tgctccatat tatgccgagg
     2221 accggaatcg aaccggtacg atcgttacca atcgcaggat tttaagtcct gtgcgtctgc
     2281 cagttccgcc accccggcct ctctaagcga acgacgggat tcgaacccgc gacccccacc
     2341 ttggcaaggt ggtgttctac cactgaacta cgttcgcact gttttcttct atctaaaaat
     2401 gccggctaca tgacttgaac acgcgaccct ctgattacaa atcagatgct ctaccaactg
     2461 agctaagccg gctcatttgt tatatcttaa tgcgggttaa gggacttgaa cccccacgcc
     2521 gttaagcgcc agatcctaaa tctggtgcgt ctgccaattc cgccaaaccc gcatatatga
     2581 cccgtactgg gctcgaacca gtgacccatt gattaaaagt caattgctct accaactgag
     2641 ctaacgagtc taaaataact tgcgttacct taaacggtcc cgacgggaat cgaacccgcg
     2701 atcttcgccg tgacagggcg acgtgataac cgctacacta cgggaccttt gggagttaac
     2761 gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaaactc
     2821 ccta
//
LOCUS       DAVLSL010000179         2819 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLSL010000179 DAVLSL010000000
VERSION     DAVLSL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.181"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(271..828)
                     /gene="sdbB"
                     /locus_tag="VXN30_001946"
     CDS             complement(271..828)
                     /gene="sdbB"
                     /locus_tag="VXN30_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1358499.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(839..1546)
                     /gene="ccdA2"
                     /locus_tag="VXN30_001947"
     CDS             complement(839..1546)
                     /gene="ccdA2"
                     /locus_tag="VXN30_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1358500.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLSVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            complement(1777..2194)
                     /locus_tag="VXN30_001948"
                     /pseudo
     CDS             complement(1777..2194)
                     /locus_tag="VXN30_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2219..2419)
                     /locus_tag="VXN30_001949"
     CDS             complement(2219..2419)
                     /locus_tag="VXN30_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358501.1"
                     /translation="MVVSASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            complement(2586..2819)
                     /locus_tag="VXN30_001950"
                     /pseudo
     CDS             complement(2586..2819)
                     /locus_tag="VXN30_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          830 a          590 c          505 g          894 t
ORIGIN      
        1 ctcaaagtaa cttccatctc tctcccaaaa ctggaagtta ctctaagacg actctgtttt
       61 agggctattt gtcgtgcact ttttctgagt cttttcgctt ttgaaccctt atatatcaag
      121 aaaaagaaaa ccagtggaag ttagtctaag acggatttcc actgatgtca cgcgtttttc
      181 tcatactaaa agctaggttg tgggaaactg gaagttagtt ttagaagtta ccaaaatctg
      241 actacatctt gtaatcagat tttatcgatt ctagttcatt tctttaaatg ctgcttctgc
      301 atccgcatta ctgatagcac caaattgaat ctttccaatc tttccttggc tatcaattaa
      361 atattctgta ggaatgcttc gaatttgata agcttggaag gtggttgctt tggtatcata
      421 aagaactggg atatccttat atccttgttc ctggaaccat tgtgggaatt gctcaacaat
      481 tttttcacct tgaattcctg gtgcaatgac agtaagaatt tcgaaatcac gatctggttt
      541 cgccgctagt tccatcaact ctggcatact tttcttgcat ggaccacacc atgaagccca
      601 aaacttcaag taaacctttt tacccttaaa atcagataac ttaacttctt tgccatccat
      661 ggattgcaat gtgaagtttg gagcatcttt tccaacagca atttgttgta cagtcgtttg
      721 ttgttttggc tgttgtgctg cttgagtctt tttagtttct tcctcaccac aggccatcaa
      781 tacaactaat gacaagagac ttaagccagc aaacattact tttttcattt gtcctccttt
      841 attcaaaaat tccagctaga acatttactt gtcctaatag taacaaaatt cccattaaaa
      901 caatgaggaa accaccaatt ttctttagta gcatcatatg acgcttgatt ttactaaaat
      961 atggcatgac tagacctgaa gctagtgcca ataccaagaa aggaagggcc atgcccagag
     1021 tgtaaatgag agtataaatc gctccttgcc aagcgccatt gcctccagaa gccgcaagtg
     1081 ctaaaacaga acttaaaact ggaccaatac aaggtgtcca accaaagcta aaggtaatac
     1141 caagtaaaaa agctgaccaa taacgattag aatctgattt tttaaaggta aaactttttt
     1201 gaacttctaa tttcttcaaa tgaaaaattt ccatctggtg aagacccaaa atgataataa
     1261 tagctcccat gccatatcga aaccaatttg catagagaat atgaccaaag taaccagcac
     1321 caaagcctag aataaagaaa atgagagaga taccagcgat aaagcaaagt gttcgaatca
     1381 agcctgacca gagaaccttt ctcccaaaca aagaaaagct ttttgcactt tcttgatcat
     1441 ccaataaaat cccagcatag actgacagaa gaggaaaaat acaaggagaa aaaaaggata
     1501 aaacacctgc tagaaaaaca gagattaaaa atactatcgt ttccaataaa gaaccaactt
     1561 tcttaataat tctaatccta ttttactata ttcaatttta tttgtaagct ttctgctacg
     1621 caaaatcgta tcgggcacta ttggaccaat cttttctttt gctagtcaag gcggatctta
     1681 tccccaaaaa tagccaaaaa gcaacgacaa ggattactcg tcgctgcttt tgtgaacgaa
     1741 aatgtctttt aggtctgaca tttcataaat catgttttac ttgagtttgt caaggattgc
     1801 tttaagctcc tctactagtt tagtttctgt ctctgctgag ccattttctt ctttcacgaa
     1861 atcaagggtt tcttggagaa ggttttgggc tttggcaagg acttttttat ccgctttttt
     1921 ctgcatctag ctgtcctaga accttggtca attccgtgct taattgctgg atttctgact
     1981 ctttcttacg gcgaattagc cagaaggcaa tcacgcctag gagggcaagt agactgacca
     2041 caatcactcc tgctggaact gagtttgttt cagtcatctt atctgaatcc ttactatctt
     2101 ccgttccttg ttttgcatcc ttcttgtcct gtgtaggctt gctgtcgcta gcatttgcta
     2161 tcacatcttt gagagagtcc aaggcagtct tcacagactc tactgcagta tgcagacctt
     2221 actctgtcaa ggcactatct tccggagctt tttgagcatc taggaggaca gccttggttg
     2281 catcgatttt cggatcagat actgttgcca aagctttcaa gcgttggtct aactcttgac
     2341 tcaaggcacg aagttcagac ttgtcaactt gctcttgagc ttgtgtgctc gttgagctag
     2401 ccgaagcgct tactaccact ctaggatctt gagtcggagc tgagcttgga gctgggacag
     2461 gacttgcagg ttgactagga gcagttatgg tatattgaaa ctagaatagt acatatggac
     2521 ttctaaaaca ttgctagaaa ttgatttgat tgtcctgatc gatttgtcct gttcttattc
     2581 cattttacta taataaccga tggtgtggtt aatgttggta agagaaattt ctgaaaccat
     2641 acttcaaaaa agtcgctcgt catcgtctct tcgtaagtca ttggagcgat taattcacca
     2701 tttgttagac ctgcaaccaa agaaatcctc tgatatcttc ttccagatac tttgcctctt
     2761 cttaactgac cttttaatga gcgaccatat tctcgataaa aataagtatc aaatcctgt
//
LOCUS       DAVLSL010000180         2815 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLSL010000180 DAVLSL010000000
VERSION     DAVLSL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.182"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..2814)
                     /locus_tag="VXN30_001951"
     CDS             complement(<1..2814)
                     /locus_tag="VXN30_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1358502.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEPSKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPS
                     AEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHN
                     FINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVI
                     KLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLKDATVEMEQEYANNTYGVQ
                     YQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVY
                     DELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENY
                     FMKVKSENFKDVMLPVTSITNDTK"
BASE COUNT          708 a          588 c          451 g         1068 t
ORIGIN      
        1 tttagtatca ttagtaatgc ttgtaactgg tagcatcaca tctttaaagt tctctgattt
       61 aactttcata aagtaatttt ctgcattact tggtaaagaa gcaagtgaag ttacatgtcg
      121 atacttatta ccatcttttg aatatagttc tacagaagat atatttctaa gttctaattt
      181 cttaataact tctttttcat ctttagcgtc atcttttttc tcatctttat gacgataaag
      241 ttcatcgtat actggtggaa tttcattacc tgctataata ctcttaatcc aagctaattg
      301 tgtaggagat aaaatcaatc cgcctgagcg attttgtgct ccattttgat gcacaccaat
      361 aacttcacca ttagcattaa atattcctcc accactcata ccaggatttc caccttgata
      421 ttgaacaccg taggtgttat tcgcatattc ttgttccatt tctaccgtag catcttttaa
      481 gtgcgtatat ttcccagctg gatatccatg aacatttagt ttgtcattaa gatttacttt
      541 cgaataattt ttaactaatt ctacatgttc tctgccagtt gtttcaggta gcttaatcac
      601 tgctaaatca tagataaatc cttttggata atctttttta ttataaaaat gaatattgtt
      661 agcagtaaat gttccttttt gtccatcagg agtcacccac tcatatacgt ttttagattt
      721 atcacctcta aattcgccag tagtattatc agtagaatta ttaatgaaat tatgtgctac
      781 tgttaaaagt acatttggtg caataaaagt agctgatcct actccagtaa ccttcgacga
      841 atctgtaata aagttcatat taattaatgc actagatttt ttctgaatac tcatttcttc
      901 tggtgtatta gtattttcat tatttggttt agtatcttct gcactaggtt gttcgatttt
      961 accagtatat tctttcagtg cttctacttt ctctggctcg actatcgctc cggcttgcac
     1021 ccctgtgtat tctggtagtg gtgctatctg ctctggttca acaatagcgc cagcctgcac
     1081 ccctgtgtat tcttttgatg gttctacttt ttctggttct actattgctc cggcttgcac
     1141 tcctgtgtat tccttcggtg cttctacctt ctctggttcg actatcgctc cagcctgcac
     1201 tcctgtgtat tccttcggtg cttctacttt ctctggctcg actatcgctc cggcttgcac
     1261 ccctgtatat tctggtagtg gtgctacctg ctctggttca acaatagcgc cagcttgtac
     1321 tccagcatat tccggttcaa ctttttcagg ttcaacaata gctccagctt gtactcctgt
     1381 atattctggt agcggttcta cctgctctgg atcacccttg tcagtcatga ctgcctctgg
     1441 taacgctggt tgaacttctg gtacgccttt atcacttact acagcttctg gcaactctgg
     1501 ttggactgct gactcacctt tgtcggttaa tacagctgct ggtagttcag gctgaattgc
     1561 gggttcaaca atagctccag actgtacttc cttatgttct gcaccagtct cagcttgttc
     1621 ctttataact tgagcttttt tagtaccttt ttcgactatt cttgaaacag gcgcagtcgt
     1681 tgaagttgaa ataatttctc gtgaaacttc ttcattgttt acagaaaata ttctgacgat
     1741 ttcaactttc ttacctaatg caccttcttg ttttactctt acagttcctt ctgctaaatc
     1801 aggattttct tgaatttctt cttgaaaatc tatttttgtt tctatagttt cgtcacgata
     1861 taagagttcc ggtttgttca actgacctga tagcacttca tcctgtggat ttaatgtatt
     1921 taccccagtc ttttcctttg gagaaatctt ctcctctttc ttcgtttcta gattcttatg
     1981 ttcggctaat tgttcttgag aatctgaaga ttgtttctct tcttttcttg gattgattaa
     2041 ttcagtagag aaaggttttt caactacttg aacttctgtc ggcttagttg aagaaacagg
     2101 tgtttgttcc tgaatagctt gtactgttga tggatggtct acaaaatttg gtgtaacatt
     2161 ataatccacc ttttgttgtt ttgtaggagt ggctgctgat ttttcttgat tacttacttc
     2221 aaaatcagaa gtcgtttttc cctctttgat atatccaata taagtgtaac ctgaaatctc
     2281 tttaggaaga ggtaattttt ctccagaggt caattcatag tccgtattgt aatttagcaa
     2341 aagatgattt tctaaagcat ggactgaaac taagacacca tttcccattc ccgcaaccaa
     2401 tactaaatgt aataccgttt tattcttaac ctttttcttg gaaacagcaa aaattaaaat
     2461 tcccagagca gctaagctag caccagcaac tagggcttgc ctctcattct tgcttccagt
     2521 atttggtaat tccgccagtt gattttgaga atttaactta taaacaagat aataagtttc
     2581 atcatcattc tccacgtatg tcggaatatc atagacaagc tgcttctttt cttctgatga
     2641 tagctctgaa tctgccacat atttatagtg aactcccgca gtttcttgag catccacaga
     2701 tgaactagct aatacagaca taaaaaataa acttgaaatc gttgcagata caagtcctac
     2761 tgataatttt ctaaatgaaa aacgctcttg tttttcacca aaatactttt ccatt
//
LOCUS       DAVLSL010000181         2773 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLSL010000181 DAVLSL010000000
VERSION     DAVLSL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.183"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..887
                     /locus_tag="VXN30_001952"
     CDS             <1..887
                     /locus_tag="VXN30_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1358503.1"
                     /translation="LAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDY
                     SLKKFDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYD
                     YDKGQILINGKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRA
                     AKLANCTDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASL
                     DVNNEKKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKS
                     KIYKNLIEKTKMAEEFIY"
     gene            898..1482
                     /locus_tag="VXN30_001953"
     CDS             898..1482
                     /locus_tag="VXN30_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1358504.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1482..2177
                     /locus_tag="VXN30_001954"
     CDS             1482..2177
                     /locus_tag="VXN30_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1358505.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VVGLIVGGLIWLK"
     gene            2165..>2773
                     /locus_tag="VXN30_001955"
     CDS             2165..>2773
                     /locus_tag="VXN30_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358506.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLM"
BASE COUNT          996 a          361 c          534 g          882 t
ORIGIN      
        1 tactagctgc tatgaagata aaagactctt tagatgcatc taaagagggc ttgatggaaa
       61 tattttattt atcgcccaaa atagaaagat taaaagaaat tcaaaatcaa gatctacaag
      121 aaggcgatga ctatagctta aaaaaatttg atattgatct aaaagatgtt gagtttgcct
      181 acaataaaga cgcaaaagtt ttaaatggtg taagttttaa agctaagcag ggagaggtca
      241 ctgctttggt aggtgcaagt ggctgcggta aaacaactat cttgaaactt atatcaagac
      301 tttatgatta tgacaaggga caaatcttaa tcaatggcaa agatataaaa gaaatatcaa
      361 cagaatctct ttttgataag gtctctatag ttttccaaga tgtggttctc tttaatcaaa
      421 gcgttatgga aaatattaga atcggaaagc aagatgcaag tgacgaagag gttaaaagag
      481 cagcaaaact tgcaaattgc acagatttta tagaaaaaat ggataaaggt ttcgatacag
      541 ttattggtga gaacggagct gagctatcag gaggagaaag acaaagatta tcaatagcca
      601 gagccttctt aaaagatgcg ccgatattga tcttagatga gataacagca agccttgatg
      661 ttaacaacga gaaaaagatt caagagtctt taaataattt agttaaagat aaaactgttg
      721 taatcatttc acatagaatg aaatccatag aaaatgcaga caagatagta gttcttcaaa
      781 acggaagagt agaaagcgaa ggtaagcatg aagagctttt acaaaaatca aaaatttaca
      841 aaaatttaat agaaaagaca aaaatggcag aagaatttat ttattaggag gactacaatg
      901 gataataaaa aattaaaagt aaaagattta gtaagcatcg gtgtttttgg cgtaatttat
      961 tttgccttca tgtttggagt tggtatgatg ggcttgattc caatattgtt cttaatatac
     1021 ccgacagtat tagccatagt tgcaggaact gttgttatgt tatttatggc taaggttcaa
     1081 aagccatggg cactatttat atttggtatg atatcaccac ttgtgatgtt tgcagctggt
     1141 catacctacg tagttgtggt tttatcactt atagtaatga taatagcaga attaattaga
     1201 aagattggta attataattc atttaaatac aatatgcttt cttatgcaat cttcagcaca
     1261 tggatatgta gctctttaat gcaaatgctt ttagcaaaag aaaaatatat ggagtggtct
     1321 ttgatgacga tgggaaaaga ttatgttgat gtattagaaa agttaataac ttatcctcac
     1381 atggctttag tagccttagg tgctttctta ggaggaattc ttggagcata tataggcaag
     1441 gctctattga aaaaacactt tgaaaaagca ggcattgtat aatgcctact ttttcttcaa
     1501 atgctccctt taatcccaat ccgatctcta agatattagt cgtatttatc acaggcttaa
     1561 ctgttatgca tagcataaac atccgttttg agctggcaat tgtttgtctt attggtattt
     1621 tgctttattt gaatggatac aaaaaaacac ttttcaaatg gattatattg tgtgggatac
     1681 tttactcctt gcctaatttt atggtgttat ctgaattaaa ccctatagtt aagatgtttt
     1741 tgagtatacc tattgttatt agaatgttta ttttaccatt tatggcagca agctttatga
     1801 taaagacctc ggatgtaggc gcaataattt catcgatgga taagcttaag atttcaaaga
     1861 atgtatccat acctattgcg gttatgttta gattcttccc atcttttaag gaggagaaga
     1921 aaaacatcaa aatggctatg agagtaagag ggataaattt taaaaaccca gtcaaatatc
     1981 ttgaatatgt ttctgtgcca ctactcatta tatcatctaa tatatcagat gacattgcaa
     2041 aagcggcaga aacaaaggca atagaaaatc caattgccaa gaccagatac attcgcgtaa
     2101 agatacagct aattgatttt gtttatgttt tagcggttgt tggacttatt gtgggaggct
     2161 taatatggtt gaaataaaaa atttaagtct tgattatggt gaagagcata tattagatga
     2221 tatatcacta tccatagccg agggagagtg cgtgctattt acaggaaaaa gtggaaatgg
     2281 taagtcatct ttaataaatt caatcaatgg actagctgta aggtatgaca acgcaaagac
     2341 aaagggcgaa atcattattg atggtaagaa tataaaaaat ttggaacttt atcaaatctc
     2401 aatgcttgtt tcaactgttt ttcaaaatcc taagacatat ttttttaatg tcaatacgac
     2461 attagaatta ttattttatt tggaaaatat cggtcttgca agagaagaga tggacaggcg
     2521 tttgaaggat atgcttaaga tattcccgat aaaaaatctt ttgaacagaa atatatttaa
     2581 tctatccggc ggtgaaaaac aaattctttg cattgcagct tcttatatag caggtacaaa
     2641 gattatagtt atggatgagc cttcatcgaa tttagatatt aaaagcataa gtgttttggc
     2701 aaagatgcta aagatattaa aagagaaagg tataagcata attgttgcag agcatagaat
     2761 ttattatttg atg
//
LOCUS       DAVLSL010000182         2746 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLSL010000182 DAVLSL010000000
VERSION     DAVLSL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.184"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            148..681
                     /gene="queF"
                     /locus_tag="VXN30_001956"
     CDS             148..681
                     /gene="queF"
                     /locus_tag="VXN30_001956"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1358507.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQINREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            743..910
                     /locus_tag="VXN30_001957"
     CDS             743..910
                     /locus_tag="VXN30_001957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358508.1"
                     /translation="MFEVVDRTDEVSLKHCFEVADRTDEVSNHTYGKVKLTWFEEIFE
                     EYHTKKPCSSR"
     gene            complement(935..1603)
                     /locus_tag="VXN30_001958"
     CDS             complement(935..1603)
                     /locus_tag="VXN30_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1358509.1"
                     /translation="MKKFVAELIGTFMLVFIGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAFSIGTVSGAHLNPAVSIAMFVNKRLSSKDLVNYILGQVVGAFIASGAVFLLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLIIGLS
                     LMAMILVGLNITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(1709..2499)
                     /locus_tag="VXN30_001959"
                     /pseudo
     CDS             complement(1709..2499)
                     /locus_tag="VXN30_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
BASE COUNT          905 a          589 c          493 g          759 t
ORIGIN      
        1 cctgttctta tttcatttta ctatataagt agaatattac gatgtttggg atatgccatc
       61 gctaaaaaaa ctcgatttgg tattttttag ctccctaatg ggagcttttt gcatgctctg
      121 aacatttccc attttggaag gagtactatg aaacgtcaat cagccttggt cgtctttagt
      181 ggtggtcaag attcaacaac ctgccttttc tgggccatgc aacactatga aacagtcgaa
      241 gctgtcacct ttgcctacgg ccaacgtcat cacctcgaaa ttcaaattaa tagagaaatc
      301 gctaaggaac agggcattcg tcaccatatc ctcgatatgt ctctgctggg acaaatcact
      361 gctcagccag actttgcgac gattcatatt tcctacattc ctgacaagct ctgtgtcgag
      421 tcaaaatccc tcaaactata tctatttagc taccgaaacc acggagattt ccacgaaaac
      481 tgtatcaaca ccatcgggaa agacttggtc aacttgctag accctcgcta tttagaagtc
      541 tgggggaaat tcactccgcg cggtggcatt tcaatcgacc cctactacaa ctacggtaag
      601 caaggaacta agtatgaggg cttggcagaa caacgcctct tccaacacga cctttatcca
      661 gagaaaattg acaaccgcta aagtcatact caatgaaaat caaagagcaa actaggaagt
      721 tagccgcagg ttactcaaag cagtgtttga ggttgtagat agaactgacg aagtcagctt
      781 aaaacactgt tttgaggttg cagatagaac tgacgaagtc agtaaccata cctacggcaa
      841 ggtgaagctg acgtggtttg aagagatttt cgaagagtat catacgaaaa agccctgttc
      901 ctcaagatga ggagcaaggc tttttgagtt tcaattattc ttctgttcca aggaaatttt
      961 ttgcaacaag ggctgcaaga actccaccag cgattggtgc aaggatgaaa atccaaactt
     1021 gttgaagggc tgcgccgcct accaagacag ctggtgccaa gctacgagct gggtttactg
     1081 aaagtccagt aatgttcaat ccgacaagga tcatcgccat caaggacaaa ccgattatca
     1141 aaccagcaat cgcgccattt cctttacttt ctgaagtcac agtcatgata accaaaacaa
     1201 acaagaaagt tgcgatgact tcaaacaaga aaccaccaaa gacagtgaca ccgtttgcca
     1261 aagcattttc accaagacta gcagttgaca tacctgaatt tgccaagagc aagaagacag
     1321 cgccagaagc aataaaagct ccgacaactt gtccaaggat gtagtttaca aggtcttttg
     1381 atgacaaacg cttgttaaca aacatagcaa tcgaaacagc tgggttcaag tgagcacctg
     1441 aaacagttcc aattgagaaa gctgcgacta cgattgccaa accaaaggca aaagcaattc
     1501 caaggtgacc aaggccatca aaaccatttc caaaaacaac agctcctgtt ccgatgaaca
     1561 caagcatgaa cgtaccgatt aactcagcaa caaatttttt cattttcttt ctcctttttt
     1621 caaaaactag atactagtct atcaaaagag ggaaagggtt tcaagaaaat tgcttggaaa
     1681 gttttttgaa aatctgactc gctttttctt agtattgaaa agactggata gcttctttca
     1741 agtcatcttg taaactattt ctctggtcaa gttgaacata gacttccaac agacaggatc
     1801 tgaagttgga aaatttataa aaatcttctc tttcttctat cggaaagtca acagctttta
     1861 tccaagaagc tacttgctct tgcgctaact tcccttgtaa aataggttca tagatcactc
     1921 ttgctaaacg ccaatcctca tcatctgtaa agcgaatcga aactctttta aatagttggc
     1981 caagtatatc aaatacttca tgaactctgt ttttaggaaa gttgggatga caaaccacct
     2041 ctgtcagtaa atcggctcca tgtgcaaaag catgaaccca accatactga cttgaaaaac
     2101 ccgttgtatc cttttctttt gaaagataat acaaaccttg atttaaaagg acattacgaa
     2161 ttcctgcatt taattcctga taaaaatcga ttgctggttg gcatctgcag acaagagatt
     2221 tgcataaata agcgccctaa aagaacgttc aagggttgac aggcctacct tatcaatatc
     2281 tttatctaac cctccatcag atgaaatccc ttcagcaatg aaatgaaatt gttcctgtgt
     2341 aaatagctct tcctgaatcc ctctagcaaa gcttgtaaaa acaaggtcat cacgaatttc
     2401 tggagaagga tctcccaaat gatcaagtag ccactggatt tcttcctggt tataacttgg
     2461 tttttcttct gctatttttc taagtaactt ttgatacatg gtcaatacct ctacatttct
     2521 agcaactgtt caaaaagctg tccttaaatg actttagcaa tttgtaaaat taagttaaag
     2581 agaaaaagac atttatattc agagaaacag cacatttctg aaaggataca taaatgattt
     2641 attccatatt accacaaatg aacttgtaat gatagaactt tatcacaaaa atcacatttc
     2701 tgtctcaaaa attgcgaaaa actagttata ataaagtgaa ataaaa
//
LOCUS       DAVLSL010000183         2666 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLSL010000183 DAVLSL010000000
VERSION     DAVLSL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.185"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(144..758)
                     /locus_tag="VXN30_001960"
     CDS             complement(144..758)
                     /locus_tag="VXN30_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1358510.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1573..1842)
                     /gene="rpsO"
                     /locus_tag="VXN30_001961"
     CDS             complement(1573..1842)
                     /gene="rpsO"
                     /locus_tag="VXN30_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1358511.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1983..2612)
                     /locus_tag="VXN30_001962"
     CDS             complement(1983..2612)
                     /locus_tag="VXN30_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1358512.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
BASE COUNT          830 a          532 c          455 g          849 t
ORIGIN      
        1 ttataagaat ctcctaatca aatcaaaagc cactctacaa tagagtaact ttaagattgt
       61 ccatttatac tcaatgaaaa agccccctga aaatcagaga gctgatttga gctcgggcta
      121 aaatcctagt gaaacaaaaa aatctacacg atcagaaaag tctctatcgt gccattttca
      181 tacatgatgt atagtccaag tagaatgaat actaagggca caatgattcg ctcgtatttt
      241 tcaattgtct cgaatattaa cggaatagag gataacaccc gactaatctc gcaaaagata
      301 attatgccga ttacaaacac aagcaaggcc acgagggtct gtgaccaatc taacgaagca
      361 aaataaggta tatagatacc taaattatct ccgccagacg caattgtcag caatgtaact
      421 gtccaaaaca gttgatttgc cttgctttgt tctaatcttt caataatttc ttcctcttct
      481 tcttccgcat cttctccaac aattgcaaag cgaatcccta aatagatagg gattaaacca
      541 agcaatccaa ccatccattc ttcaggcacg aaattaacga cataagcagc aactaaactc
      601 gcccctacaa gtaagcctgt gcctagatat tgccccgcat aaatatgcca tttctgttta
      661 ttctgtgata gctgtgcaaa taaaataatt aaaataatta aataatcgat actggtggaa
      721 atataaacac caatagcaga tatgattgtc tgtcccataa aaaaaaactc ctgtattagt
      781 cagtaataaa tcagcagatt ttaggagagc acagacacat taatttagct atcgctagtg
      841 ggtaatgtcg aacgtaggaa agcattttac tcctcctcaa aacgtagtta caaagtaatt
      901 tctaactgga attcagtgac tacttccatg taaagtataa catactatac tttactttgt
      961 aaagagtggg ctcttctagg gggcttgcat acagctactc ggcttgaaaa gccgagtaac
     1021 tgcgcacctt tatggtgtaa ttagctgata ccatttgagg tttttgtagt ctccaaaatt
     1081 gttaccgata aaacctcaat aaaaaagccc tctgaagtca gagagctgat tttagcccgg
     1141 gctaaaatcc tagtgaaaaa gatgaaactc cttgtgctca tcgaacactg tgtcatttcc
     1201 ctattttcat acggattttt gacgccctta gcatcatgat tcttgctaga taaatcgttt
     1261 atagactagg ggacgaggtg aagatcgtac gaaactttca gtgaaacaaa aaaatctccc
     1321 cgaagggaga aaagctagtt tattttacct tacagtgcta ggaaccgtaa ccgaagatta
     1381 tacttgagta tagcgaggta aggtgacaac gcaacaaagc tacccaattt agagtagctt
     1441 tattatcaag atatgctcaa ttgaacacgg cctaagcact tggcaaaaaa gataaaatct
     1501 cctagaaact gaagtttctt cgtcaatttt cctatttttg ctttgtgttt ttgacggcct
     1561 ttgtatcttg aattagcgac gaagtcctag agagttgatc aactcacggt aacggttaac
     1621 gtcgttttta cgcaagtatg caagcaagtt acgacggcga ccgatttttt tcatcaatcc
     1681 acggtaagta gcgtggtctt ttttgtgttg tttgatgtgt tcgttaaggt ggttgatttc
     1741 ccaagtaagg acagcaactt gaacctctac tgaacctgta tcaccttcgt gacgtgcata
     1801 ttgtgcaatg atttcatttt ttttctcttt tgagattgcc atgatgtttc tcctttttat
     1861 ttggcttcat ccgagtgaca ggttggcatg cctgtaacca agaagaagtt atttgtcttt
     1921 acgacaatcc ctattttacc aagatttaaa ttttctgtca aacattaata ctctcatagt
     1981 tattattttt caagtaaaga gatacgttct tttatatgat gacctttttc aagttcatct
     2041 accaatccaa tagcataatc tgcataacta atctggctta ttcctttttc attagtagta
     2101 aaaatttctc ctgctagaat gtagtttcct gttttttcac catcgggaat aaaatctaca
     2161 gcaggactga caaaagtcca gttgagatta ttttttgtac gaagaagatc caattcatcc
     2221 gcttgagctt ttgctagtga cttaaattct tcaggaaaat cagacgtatc taaaagtcgt
     2281 gtcgtttttg tttcatctat ataaagactt cctgctcccc ctacaactag aaaacgtgtc
     2341 tgtgttccag caagaagctg attaaatagt tcgattgatt tgctgtggag cggtagcgta
     2401 tctggtgtat aagcaccaaa cgctgaaata acagcatcaa atccagtaag atcatctttt
     2461 gtcaactcaa ataaatcttt tttaataata gactcagatt gacttttgtt ttcagaacga
     2521 acaatagccg ttacttcatg tcctcgtttg actacttctt caacaattgc tttccccgct
     2581 tgtccatttg ctgcaataac tgctagtttc attttttata cctctcttat tgtaattatt
     2641 ttagttacaa caattgtgac actctt
//
LOCUS       DAVLSL010000184         2664 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLSL010000184 DAVLSL010000000
VERSION     DAVLSL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.186"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..615
                     /locus_tag="VXN30_001963"
     CDS             <1..615
                     /locus_tag="VXN30_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEV1358513.1"
                     /translation="TSTLSNLWSGLVSTGSNLFNNLSGTISGIFNGILSTASNIWNSI
                     KSTISNAIDGAKNAVSNAIQAIKNLFNFNISWPHIPLPHFYVSGSANPLDWLSQGVPS
                     IGIEWYAKGGIMTKPTLFGMNGNRAMVGGEAGAEAILPLNKSTLGAIGQSIANTMNTS
                     NSINVNFSGVTIREEADLNRLADVVGTRIAEELQRKTNLRGGFA"
     gene            612..1334
                     /locus_tag="VXN30_001964"
     CDS             612..1334
                     /locus_tag="VXN30_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1358514.1"
                     /translation="MTKINELIIDGVKTSSFKCEILVETRPQIIVSSSKTSLLEHDGI
                     SGAIVQSNRHRRLIEKSYHISLINPTDEDLYRFSSLLNREKFWLENEQEPSVKYWCYK
                     VDDFKIIKDDFGAWTVDVKFTCHPTKYFKGSDTQRLTRSGTLTVQGSALAFPKITIVG
                     QSAVETSFTIAGQVIRLEKLSESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSLEIETVWGWA"
     gene            1335..>2664
                     /locus_tag="VXN30_001965"
     CDS             1335..>2664
                     /locus_tag="VXN30_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1358515.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKETMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEYNDHLEITAYHISDDVMQRSITPVS
                     VTSQSCGLTLSRMVQNTKTALGDFSFNSDIQDRRTFNTTETETLYSILLDGKHSIVGT
                     WEGELVRDNFAMTVKKSRGENRGVVITTHKNLKNYQRTKNSQNVVTRIHAKSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKTVEELQKWAQSKFSNEGIDKVSDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDAFKKAIAYEFDALKEEYISLTFDDKAGIGG
                     SRASGGLSSAADAILGVTESAQEIALEKALQNADLDFDHKAGLLRQEISDDIELAKAR
                     AEEVKRELSDTINQRFNSFDNGPLKETKRKAEEALRNAGASSSLAQESKRI"
BASE COUNT          837 a          494 c          594 g          739 t
ORIGIN      
        1 acttctaccc tttccaattt atggtctggc ctggtctcaa ccgggtcaaa tttgtttaat
       61 aatttgagtg gcacgatttc aggaattttt aatggtatct tatccactgc tagcaatatt
      121 tggaactcta tcaaatcaac tatttcaaat gctattgatg gtgctaaaaa tgcagtatct
      181 aacgctatcc aagctattaa gaatctattt aatttcaata tcagttggcc gcacattcca
      241 ttaccacact tctatgtaag tggttcagcc aatccattag actggttaag tcagggcgtt
      301 ccaagcatcg gtattgagtg gtatgcgaag ggtggtatca tgaccaaacc aactttattt
      361 ggaatgaatg gaaatagagc aatggttggt ggagaagctg gtgcagaagc aatccttcca
      421 ctcaataagt caactcttgg tgcaattgga caaagcattg ctaatacgat gaatacatcg
      481 aatagcatca atgtcaactt ctcaggagtg accatccgag aagaagcgga tttgaataga
      541 ctagctgacg tagtcggaac acgtattgct gaagaactac aaagaaaaac taatttgaga
      601 ggaggtttcg catgacaaaa attaatgagt taatcattga tggagtgaaa acatcatcat
      661 ttaaatgtga gattctggtt gaaacacgac cacaaatcat cgtatcctcc tcaaaaacta
      721 gtcttttaga acatgatggg attagtggtg caattgttca atcaaatagg catcgtaggt
      781 tgattgaaaa aagctaccac attagcttga ttaacccaac ggatgaagac ttataccgtt
      841 tttcttctct gttaaatcgt gaaaaatttt ggttagagaa tgaacaagag ccaagcgtga
      901 aatattggtg ctataaagtg gatgatttca aaattattaa agatgatttt ggtgcatgga
      961 cggtggatgt aaaattcact tgccatccta caaaatactt taaaggctcc gatacacaga
     1021 gattgacaag aagtggaacc ttgaccgttc aaggttctgc tcttgctttt cctaaaatca
     1081 caatcgttgg tcagagcgct gttgagactt catttacaat cgctggtcag gtcattaggc
     1141 ttgaaaaact ctcagaatcg cttgtgatgg ttaataatcc tgacaatcct agctttaaaa
     1201 cgacaacagg gaagccagtg aaatggtcag gggattttat cacagttgat ccagcgaaag
     1261 tgaagaatat tggggttgtt ttgggtccag gtattcaatc acttgaaatt gagacggttt
     1321 gggggtgggc ataattgctt tatctactta ataaagatgt gagaaccgtt cggtggaacg
     1381 gggagccact tcatgaagcg acttcggcga ttgttaaaga gaccatgaat ggcgatttca
     1441 ccttaactgt gaaatatccc atttccgact ctggtattta tcagctcatc caagaagata
     1501 tgttgataaa agcgccgact cctgttcttg gtgcgcagct atttcgcatt aagaaacctg
     1561 ttgaatacaa tgatcatctg gaaatcacag cctatcacat ttcagacgat gtgatgcaac
     1621 gttctatcac accagtaagt gtgactagtc agagctgtgg cttgactctt tctcgcatgg
     1681 ttcaaaacac caaaactgct ttgggagatt tttctttcaa tagcgatatc caggaccgta
     1741 ggaccttcaa tacgactgaa acagaaaccc tatactctat attgctggac ggtaagcaca
     1801 gcattgttgg tacatgggaa ggcgagctgg ttcgtgataa ctttgcgatg actgtcaaga
     1861 agagtcgtgg ggagaatcgt ggtgttgtta ttacaacgca caaaaatctg aagaactacc
     1921 aacgcacaaa aaacagtcag aatgttgtca caagaattca cgcaaagtcg acttttaaac
     1981 ctgaaggtgc tgaaaaagaa acgactatca gagtgactgt tgatagtcct cttatcaact
     2041 cataccctta tatcaatgaa aaagagtatg agaacaacaa tgctaaaact gttgaagagt
     2101 tgcagaagtg ggcacagtct aagttctcaa atgagggaat tgacaaggtc tctgatgcta
     2161 tcaagattga agcttatgaa cttgatgggc aagtggtcca tatgggcgac acggtcaatc
     2221 tcaagagctg gaaacataat gtcgatgcat tcaagaaagc tattgcttat gagttcgatg
     2281 ccttaaaaga agaatacatc tctctgactt tcgatgataa ggcaggtatt ggtggttcta
     2341 gagcttctgg tggcctatct agcgcagccg atgcaattct tggagtgaca gaatctgcac
     2401 aagaaatcgc ccttgaaaag gctcttcaaa atgctgactt agactttgat cataaggctg
     2461 gattgcttag acaggaaatt tctgacgata ttgaactggc caaagccaga gctgaagaag
     2521 tcaagagaga actgtctgac actatcaatc agcgatttaa tagctttgac aacgggccat
     2581 tgaaagaaac taagcgcaag gctgaggaag ctttgcgaaa tgctggcgca agtagttctc
     2641 ttgctcagga atccaagcgg attg
//
LOCUS       DAVLSL010000185         2650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLSL010000185 DAVLSL010000000
VERSION     DAVLSL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.187"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..206)
                     /locus_tag="VXN30_001966"
     CDS             complement(<1..206)
                     /locus_tag="VXN30_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1358516.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEIL"
     gene            complement(374..1018)
                     /gene="pcp"
                     /locus_tag="VXN30_001967"
     CDS             complement(374..1018)
                     /gene="pcp"
                     /locus_tag="VXN30_001967"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1358517.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1033..1956)
                     /locus_tag="VXN30_001968"
     CDS             complement(1033..1956)
                     /locus_tag="VXN30_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1358518.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(1953..2642)
                     /locus_tag="VXN30_001969"
     CDS             complement(1953..2642)
                     /locus_tag="VXN30_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1358519.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
BASE COUNT          866 a          586 c          394 g          804 t
ORIGIN      
        1 atcaaaattt ccacagcttt ttccaatttt ttataaaaac tatcgacatt cccattcttt
       61 ataatggcaa attgtccgtc taattcggca atttctccga taactttttt accgatagtc
      121 aatgtataac cttcaaaact gtctttacca acattaactt tggcatccgc tacttgaatt
      181 tcgatttttt tatctttctt gctcatttca tacctctctt cactttttct attttacaag
      241 aaaatcctaa aactagcaag aaaaaactct atatcaggct tctctgatat agagttttat
      301 gtaaacaacc tcaatcgatt tagcatagct aatagttagt aaacaagcat aattgactat
      361 cctttttagg ttatcagtga gtctcaccgc ctactaactg caaatcctta tctttgtagt
      421 ctacaatagc ttcaatagct acctctatgc ctcttacaat atcacataga ctcattcctg
      481 cagtattcgg cttatttact acttgttcca tcatatatgg aatatgcatg aaacctgctc
      541 tcatattagg gaatttttta tccactaaat aaagagcttg gtacatcaaa tgattgcaaa
      601 caaaggttcc tgcactattg gatacaactg ccggaagtcc ctgttttttg atagcttgta
      661 ccatcgcttt gataggtaaa ctactaaaat aggccgatgc tccatcaata cgaatcggtg
      721 tatcaattgg ttgattgcct tcgttatcag gtatgcgagc atcatcttga ttaatagcca
      781 ctcgttcagg tgttaagccg gtcctgccgc ctgcttgtcc aatacaaagt acagcatctg
      841 gttgatatcg taatatttct gcctctaaaa cttctgacga cttataaaaa accgttggaa
      901 tttctaccca gcgaacttca gccccattaa tctcagatgg taataatttt acagcctcca
      961 aagctggatt aatcttttca cctccaaaag gattaaaacc tgtaaccaat attttcattt
     1021 tattttcctt tactaaaatg cgagaaagta cattaagaat atgtgaataa caatcattac
     1081 tagagcaaca cctgcttgag cctttataac gccattctga tctttcatat ccatcaatgc
     1141 tgctggtaga gcgttaaaat tagcagccat tggggtcaat aaggtcccac aataacctgc
     1201 tgtcatggca agagcaccag ccacaattgg attagctccc agagcaaata caaagggaac
     1261 tccaacacct gctgtaataa cggtgaatgc tgcaaaagca tttcccataa tcattgtgaa
     1321 tagaaccatt ccaagaacat aggccaaaac tcctataaag cgactatctg aaggaacaat
     1381 accgctaatc agatgagaga taacatcacc aacacctgct acagtaaaaa tagcccccaa
     1441 agcccctaat aattgaggaa caataccact tgttgaaact tgctgagtca ttcgattatt
     1501 ttctgataac agactcttag ggtgactatt ggtaatcaca agaacagaaa ttgtagcaaa
     1561 caaggcggca aggctaatcg aaatcttgct aaattctgga atcatttgcg ctaagaccaa
     1621 cgcaagtatt gccatcagca taactggaat aaaaatttta tttttcaacc tgttagattc
     1681 aatattggct ttcatttcat ctaaggatgg caaggttccg atacggactt gcttaaacaa
     1741 tgttaacagc gataatagga ttacaataat accaatactc atatttggca tataggaacc
     1801 acctataaac gtaatagaca atagagtcca aaatgcagat gtcccaagtc gaactgggtt
     1861 tgttttatct ttataactac aataggctgt atggagaaat tgacaaccaa tcacaatata
     1921 ggtcaactct aatagttgct ttgccaactc tgtcattttt gttctcctcc cctagtcttt
     1981 tttgatatca attttttatc aaataaataa ttgtaaatcc ccactacaat aagtgttata
     2041 acagcaacaa taatagatgt agaagcaatc cctgcataat tgctttcata gcctaactga
     2101 tctaatgttc cccctatcaa gaggactccc ccagcaccta caaacgtatt ttgagcaaag
     2161 aaatttccaa aattttcatt cgcagccgca cgcgctttta ttgtctcatc ttcaacctct
     2221 gttaactttc tacctaattg agactctgca gctgcttctc ccataggttg aaccaaaggt
     2281 ctgacaaact gagggtgtcc tcctagacga attgaaaaga aaccagctaa ctctcgaata
     2341 aaaaaataaa ctgtatagaa gtttccaact gtcagacctt taatctttcg aatcaaatcg
     2401 attgatcgtt gcttgagtcc aaaggtttct gacagcccca caagaggcaa ggtaaccata
     2461 aaaatcgtga gcactcgctg attgctaaat tcttttccca aaatctccaa aaattcaacg
     2521 agagaaacac ctgaaactaa agctgtaacc aaaccagcta agactactgt tgcaattgta
     2581 tcaaatttta aaataaaacc cacaacaatg attgctattc ctattaatct aatccactcc
     2641 atatcaaact
//
LOCUS       DAVLSL010000186         2647 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLSL010000186 DAVLSL010000000
VERSION     DAVLSL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.188"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(93..332)
                     /locus_tag="VXN30_001970"
     CDS             complement(93..332)
                     /locus_tag="VXN30_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1358520.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(352..624)
                     /gene="rpsP"
                     /locus_tag="VXN30_001971"
     CDS             complement(352..624)
                     /gene="rpsP"
                     /locus_tag="VXN30_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1358521.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            794..1003
                     /locus_tag="VXN30_001972"
     CDS             794..1003
                     /locus_tag="VXN30_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358522.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            1482..1703
                     /locus_tag="VXN30_001973"
     CDS             1482..1703
                     /locus_tag="VXN30_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358523.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            1787..2590
                     /locus_tag="VXN30_001974"
     CDS             1787..2590
                     /locus_tag="VXN30_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1358524.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
BASE COUNT          829 a          525 c          471 g          822 t
ORIGIN      
        1 tttgactgtc ctgatcgatt tgtcctgtta ttatttcatt ttactatatc ttctattcca
       61 cacaaaaaag cgagacatcc gtcccgccct tcttattttt cgtcaataac gattcttact
      121 tttttgtatt cagttgggac agagtagaca atcgttctta tcgcagaaat agtgcgaccc
      181 ttacgaccga ttacacgacc cacatcgctt tgatcaagat tcaaatgata ttccaaaaat
      241 tctggtgtat cctcaatctt gatagttaag gcatctggtt gtgaaattaa gggtttcaca
      301 atcgcaataa tgagattttc aatcgtatcc atctgtcaac ctactttaaa cttattttga
      361 aaatttagaa tcgtggaatt ttttcaatac gccttctttt gaaaggatgt tacgtactgt
      421 gtctgaaggt tgagctccat tagccaacca tgcaagaacg cggtcttctt tcaaagttac
      481 ttggttttca gcaacaagtg ggttgtaagt tccaactgtt tcgatgaaac gtccgtcacg
      541 tggtgaacgt gaatctgcta cgttgatacg gtagaaaggt tttttcttag aacccatacg
      601 agtcaaacgg attttaactg ccatttttaa agtctcattt ctttaatttt ttatttcggt
      661 gaaatagctg agctatctag cacatgttct attatagcag atttctggca tgtgtcaaga
      721 aaaaaacttg acagactata aatttcttaa agtatttaga aatttgttag aataggaaat
      781 aaatgtttga aagatgctat ccgtgaatac tattttagaa aaattttata aagaacacca
      841 agtcaaaccc ttcatatcgc ctgaacgaga gttggatact tggcttctaa gtcccaagcc
      901 cgttcccaag cgaaatatgg acttattagt agatgattca ctagcaggag atatcatttt
      961 actatggaga atccagtttg gtacttttac cactgaaaca tgattttaaa aactcccatt
     1021 cgaatatgat tcaaaattcg cttagaatca atgttttccc actttttcaa aaaataaatt
     1081 ttcatctaat ttaaataact ttcaattaag tgtgaatttt gcttaaaatc attaaaaaac
     1141 accccatggt gtgaaccatg aagtgttgta aatgctgtgt tgtagctagt tcttagcgtt
     1201 tagagaactg gctagcttta cgagctttct taagatctgg tttccaaaat actttgagta
     1261 cacttatggt attgatgccc ctaaacatct aaaaactctg gtagaaaaag ggtatgctct
     1321 tgtcgaaact tcttttgact cacttgacca tctaaatact accatgaaaa agaatatcct
     1381 gaagaagaag ggagttactg gactttctaa aatgaaatct tgcgacctgg atcaagccct
     1441 tcatgagcat ttttcagaag aagaattagc tggtcacttt catgtccttc tatggacttt
     1501 tttacaatgg cattgctatc acacccaata cctatctaag cgcctggttc gtaaacttta
     1561 ttgcagctct tcctctaaat ttcctaattg ttgaaccaat tgcccgtttt atactaagtt
     1621 cttttcagaa accatttact ggggaagaag ttgaagaagt tgaagatttt caagatgatg
     1681 atgaaatccc aactattatc taagccagtt ctgtaaacta ctaatatttg aaatccactt
     1741 ccttttaggg tacaatggtt ataaatgaat ttttgagagg atcagaatga aaaaactagc
     1801 aacccttctt ttactgtcta ctgtagccct agctgggtgt agcagcgtcc aacgcagtct
     1861 gcgtggtgat gattatgttg attccagtct tgctgctgaa gaaagttcca aagtagctgc
     1921 ccaatctgcc aaggagttaa acgatgcttt aacaaacgaa aacgccaatt tcccacaact
     1981 atctaaggaa gttgctgaag atgaagccga agtgattctc cacacaagcc aaggtgatat
     2041 tcgcattaaa ctcttcccta aactcgctcc tctagcggtt gaaaatttcc tcactcacgc
     2101 caaagaaggc tactataacg gtattacctt ccaccgtgtc atcgatggct ttatggtcca
     2161 aactggagat ccaaaagggg acggtacagg tggtcagtcc atatggcatg acaaggataa
     2221 gactaaagac aaaggaactg gtttcaagaa cgagattact ccttatttgt ataacatccg
     2281 tggtgctctt gctatggcta atactggtca accaaacacc aatggcagcc agttcttcat
     2341 caaccaaaac tctacagata cctcttctaa actccctaca agcaagtatc cacagaaaat
     2401 tatcgaagcc tacaaagaag gtggaaaccc tagtctagat ggcaaacacc cagtctttgg
     2461 tcaagtgatt gacggtatgg atgttgtgga taagattgct aaagccgaaa aagatgaaaa
     2521 agacaagcca actactgcta tcacaatcga cagcatcgaa gtggtgaaag actacgattt
     2581 taaatcttaa aaaccaaaaa aatacagtat ccacattcgg tactgtattt ctggctctat
     2641 aatatct
//
LOCUS       DAVLSL010000187         2615 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLSL010000187 DAVLSL010000000
VERSION     DAVLSL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.189"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            34..105
                     /locus_tag="VXN30_001975"
     tRNA            34..105
                     /locus_tag="VXN30_001975"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66..68,aa:Gln,seq:ttg)
     gene            267..1469
                     /gene="rpsA"
                     /locus_tag="VXN30_001976"
     CDS             267..1469
                     /gene="rpsA"
                     /locus_tag="VXN30_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1358525.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1554..2615)
                     /locus_tag="VXN30_001977"
                     /pseudo
     CDS             complement(1554..2615)
                     /locus_tag="VXN30_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          794 a          440 c          598 g          783 t
ORIGIN      
        1 ggggttcgaa tccctctctc tccatttcat taatggggta tagccaagcg gtaaggcaag
       61 ggactttgac tccctcatgc gttggttcga atccagctac cccagttctt aggtaataat
      121 caagataaaa agcaaaatat cttagggtat tttattttta taattgaaag acgtgaatga
      181 tatgaacatg tccttgcggg tgcttaggaa aaaaattata agtatgtcaa gtttaagaaa
      241 aacttgattg ttggaggatt ttttagatga acgaatttga agatttgcta aatagcgtta
      301 gtcaagttga gactggtgat gttgttagtg ctgaagtatt gacagttgat gcgactcaag
      361 ctaacgttgc aatctctgga actggtgttg aaggtgtctt gactcttcgc gaattgacaa
      421 acgatcgtga tgcagatatc aatgactttg ttaaagtagg agaagtattg gatgttcttg
      481 tacttcgtca agtagttggt aaagatactg atacagttac ataccttgta tctaaaaaac
      541 gccttgaagc tcgcaaagca tgggacaaac ttgttggtcg cgaagaagaa gttgttactg
      601 ttaaaggaac gcgtgccgtt aaaggtggac tttcagtaga atttgaaggt gttcgtggat
      661 ttatcccagc ttcaatgttg gatactcgtt tcgtacgtaa cgctgagcgt tttgtaggtc
      721 aagaatttga tactaaaatc aaagaagtta acgctaaaga aaaccgcttc atcctttcac
      781 gtcgtgaagt tgttgaagca gctactgcag cagctcgcgc tgaagtattc ggtaaattgg
      841 ctgttggtga tgttgtaact ggtaaagttg ctcgtatcac aagcttcggc gctttcatcg
      901 accttggtgg tgttgacgga ttggttcact tgactgaatt gtcacatgaa cgtaacgtat
      961 caccaaaatc agttgtaact gttggtgaag aaattgaagt gaaaatcctt gatcttaacg
     1021 aagaagaagg acgtgtatca ctttcactta aagcaacagt accaggacca tgggatggcg
     1081 ttgagcaaaa attggctaaa ggtgatgtag tagaaggaac agttaaacgt ttgactgact
     1141 tcggtgcatt tgttgaagta ttgccaggta tcgatggact tgttcacgta tcacaaattt
     1201 cacacaaacg gattgaaaat ccaaaagaag ctcttaaagt tggtcaagaa gttcaagtta
     1261 aagttcttga agttaacgca gatgcagaac gcgtgtcact ttctattaaa gctcttgaag
     1321 aacgtccagc ccaagaagaa ggacaaaaag aagaaaaacg tgctgctcgt ccacgtcgtc
     1381 caagacgtca agaaaagcgt gatttcgaac ttccagaaac acaaacagga ttttcaatgg
     1441 ctgatttgtt tggtgatatc gaactttaat caaattgaaa attcacaaaa tcctttgttt
     1501 actaaacaag ggatttttct ggctctttgt caactgtagt gggttgaagt cagctaagct
     1561 tgagaaagga caaatttcgt cctttctttt ttgatgttca aagcgataaa aatccgtttt
     1621 ttgaagtttt caaagtttcg aaaaccaaag gcattgcgct tgataagttt gatgagatta
     1681 ttggtcgctt ccagtttggc attagaatag tgtagttgaa gggcgttgat aatcttttct
     1741 ttatctttga ggaaggtttt aaagacagtc tgaaaaatag gatgagcctg cttaagattg
     1801 tcctcaataa gtccgaaaaa tttctctggt tccttattct ggaagtgaaa cagcaagagc
     1861 tgatagagct gatagtggtg tttcaagtct tctgaatagc tcaaaagctt gtctaaaatc
     1921 tctttattgg ttaaatgcat acgaaaagta ggacgataaa atcgcttatc actcagttta
     1981 cggctatcct gttgtatgag cttccagtag cgcttgatag ccttgtattc atgggatttt
     2041 cgatgaaact gattcatgat ttgaacacgc acacgactca tagcacggct aagatgttgc
     2101 acaatgtgaa agcgatcaag aacgatttta gcattcggga gtgaaacagt ctgggagact
     2161 gtttcagcct gagcctagaa atttgaaagc gaagctgttt agccaagtca tagtaagggc
     2221 taaacatatc catagtaata attttgacgc gacatcggac agctctatcg tagcgaagaa
     2281 agtgatttcg aatgatagct tgtgttcttc cctcaagaac agtgatgata ttgagattgt
     2341 taaaatcttg cgcaatgaag ctcatctcca tctcccgatt gaaacagtca ctccccggac
     2401 tgtttcaacg tcccaggaca taatctcagg aagacgcgaa aaatcatgct caaagtgaga
     2461 atcattgagc ttgcgaatga cagttgaagt tgagatggaa agctgatggg caatatcagt
     2521 catagaaatc ttttcaatta acttttgagc aattttttgg ttgatgatac gagggatttg
     2581 gtgatttttc tttaccaggg gagtctcagc aacca
//
LOCUS       DAVLSL010000188         2434 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLSL010000188 DAVLSL010000000
VERSION     DAVLSL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.190"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            49..966
                     /gene="adcAII"
                     /locus_tag="VXN30_001978"
     CDS             49..966
                     /gene="adcAII"
                     /locus_tag="VXN30_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1358526.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            974..>2434
                     /gene="phtD"
                     /locus_tag="VXN30_001979"
     CDS             974..>2434
                     /gene="phtD"
                     /locus_tag="VXN30_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV1358527.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTVRGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRK
                     VGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKIDLPSSDREFY
                     NKAYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDV"
BASE COUNT          804 a          466 c          516 g          648 t
ORIGIN      
        1 tcaggagtag aatatttact agttaattaa atggttaagg agttgttcat gaagaaacaa
       61 aatttatttt tagtcctgtt aagtgtcttt cttttgtgct tgggggcttg tggtcaaaag
      121 gaaagtcaga caggaaaggg gatgaaaatt gtgaccagtt tttatcctat ctacgctatg
      181 gttaaggaag tatctggtga cttgaatgat gttcggatga ttcagtcaag tagtggtatt
      241 cactcctttg aaccttcggc aaatgatatc gcagccatct atgatgcaga tgtctttgtt
      301 taccattctc atacactcga atcttgggca ggaagtctgg atccaaatct aaaaaaatcc
      361 aaagtgaagg tcttagaggc ttctgaggga atgaccttgg aacgtgtccc tggactagag
      421 gatgtggaag caggggatgg agttgatgaa aaaacgctct atgaccctca cacatggcta
      481 gatcctgaaa aagctggcga agaagctcag attatcgctg ataaactttc agaggtggat
      541 agtgagcata aagagactta tcaaaaaaat gcgcaagcct ttatcaaaaa agctcaggaa
      601 ttgactaaga aattccaacc aaaatttgaa aaagcgactc agaaaacatt tgtaacacaa
      661 catacagcct tttcttatct agcgaagaga tttgggctta atcaacttgg tattgcaggt
      721 atctctcctg aacaagaacc aagtccacga caactaacag aaattcagga atttgttaag
      781 acctataagg ttaaaacgat ttttacagaa agtaacgctt cttcaaaagt agctgaaact
      841 cttgtcaaat caacaggtgt gggtcttaaa actctgaatc ctttagagtc agacccacaa
      901 aatgacaaga cctatttaga aaatcttgaa gaaaatatga gtattctagc agaagaatta
      961 aagtgaggaa agaatgaaaa tcaataaaaa atatctagca ggttcagtgg cagtccttgc
     1021 cctaagtgtt tgttcctatg aacttggtcg tcaccaagct ggtcaggtta agaaagagtc
     1081 taatcgagtt tcttatatag atggtgatca ggctggtcaa aaggcagaaa atttgacacc
     1141 agatgaagtc agtaagagag aggggatcaa cgccgaacaa attgttatca agattacgga
     1201 tcaaggttat gtgacctctc atggagacca ttatcattac tataatggca aggttcctta
     1261 tgatgccatc atcagtgaag aacttctcat gaaagatccg aattatcagt tgaaggattc
     1321 agacattgtc aatgaaatca agggtggcta tgtgattaag gtagacggaa aatactatgt
     1381 ttaccttaaa gatgcggccc atgcggacaa tattcggaca aaagaagaga ttaaacgtca
     1441 gaagcaggaa cgcagtcata atcacaactc aagagcagat aatgctgttg ctgcagccag
     1501 agcccaagga cgttatacaa cggatgatgg gtatatcttc aatgcttctg atatcattga
     1561 agatactggt gatgcttata tcgttcctca cggcgaccat taccattaca ttcctaagag
     1621 tgatttgtct gccagtgaat tagctgctgc ccaagcctat tggaatggga agcagggatc
     1681 tcgtccttct tcaagttcta gtcataatgc aaatccagct caaccaagat tgtcagagaa
     1741 ccacaatctg actgtcactc caacttatca tcaaaatcaa ggggaaaaca tttcaagcct
     1801 tttacgtgaa ttgtatgcta aacccttatc agaacgccat gtagaatctg atggccttat
     1861 tttcgaccca gcgcaaatca caagtcgaac cgtcagaggt gtagctgtcc ctcatggtaa
     1921 ccattaccac tttatccctt atgaacaaat gtctgaattg gaaaaacgaa ttgctcgtat
     1981 tattcccctt cgttatcgtt caaaccattg ggtaccagat tcaagaccag aacaaccaag
     2041 tccacaatcg actccggaac ctagtccaag tccgcaacct gcaccaaatc ctcaaccagc
     2101 tccaagcaat ccaattgatg agaaattggt caaagaagct gttcgaaaag taggcgatgg
     2161 ttatgtcttt gaggagaatg gagtttctcg ttatatccca gccaaggatc tttcagcaga
     2221 aacagcagca ggcattgata gcaaactggc caagcaggaa agtttatctc ataagctagg
     2281 agctaagaaa attgacctcc catctagtga tcgagaattt tacaataagg cttatgactt
     2341 actagcaaga attcaccaag atttacttga taataaaggt cgacaagttg attttgaggc
     2401 tttggataac ctgttggaac gactcaagga tgtc
//
LOCUS       DAVLSL010000189         2431 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLSL010000189 DAVLSL010000000
VERSION     DAVLSL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.191"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(371..1264)
                     /locus_tag="VXN30_001980"
     CDS             complement(371..1264)
                     /locus_tag="VXN30_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358528.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(2018..2248)
                     /gene="blpU"
                     /locus_tag="VXN30_001981"
     CDS             complement(2018..2248)
                     /gene="blpU"
                     /locus_tag="VXN30_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV1358529.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
BASE COUNT          805 a          507 c          265 g          854 t
ORIGIN      
        1 cactaagact caataaaaga taatgtctat ttaactttta tacgattact acctcctttt
       61 tagtttacac ttcctacatc ataaataaac atactcaacc ctcacaatct ccaaataact
      121 cttctatatc attgaaaatt gagtttcatc ctctatacta atgtaggaaa cctattaaaa
      181 cattccttta atccaatata agaatacctt gttccattaa tctctacccg atattataga
      241 tagaaccaaa ttcctatact taaaaggaca aataacgttc cacccataat aaacctcctt
      301 tctttttgag agcgttttct ttataggttt agtatagcaa aaacatgaaa aaaacaacat
      361 gacttagaag tcaggttgtt gggaaatcta tctgtttttc tgctaaaatt ctctgaacta
      421 atacctgatc gtctagcaat tctgctagag aaatcgcctt ttcatagaaa ctttctgcct
      481 ctaaaatatt cttgtctaca aataaagcat attttgcctt tagcaaaaat agatgaggtc
      541 ggtaaaaaac atgtcttgtt ttagcaataa ttttctcaaa tgtttgaata tatatcagac
      601 tatcttctgt ctgaatttgg ataaataggc tcagaagaac tttttccatt tgaactaggc
      661 tagccttatc ttcatattct gaacatagca atactttttg agctaactcc tcaaaatgtt
      721 ctttatcttc taatcccata gcacaacaaa agaaatacaa ttcaataatc aacaaatcat
      781 ttacagaaaa atatttcttt tttaataact gctggaaata ttcttccact aaccctaaac
      841 cgtaggtcac atctgaacta ccataaatat caaaacgagc ttgtgtcaca tccacaatta
      901 actgctcttc ctcaggcaaa ttctcataat agttttcata tatttcctca agtatagctt
      961 cttttcgttc gattcttccc ttatcagcgt aggtttgaga atgaatcagc tcatctttga
     1021 gttttaggaa tccttttgga atctctataa ccttaacatc cacaatattc tgtaatgata
     1081 ctccaagttt atccgcaatc aataataatt taggaagtgt tgctaaagct tgcccatttt
     1141 caatacgtgc tagctgacga atcgtcaact ctgattcatc accacaaata tctatctgag
     1201 tttttccaca agatcgccta atagttttga ttttataacc aatttcacgt ttcaactcat
     1261 ccatagtaat actccttaag ttgattacaa agatttcttt ttcttcctaa ttcttttgtc
     1321 aatcatatat aaaacgataa gcgaaaacag cggtagaaca atataactct tttcactcat
     1381 tatcagctga aatcccaggg ttgctagtat cttaaagatg aaaatattca tcaaaaatag
     1441 aaaaacaaat actaaaaaat attttaaaac cttcttcatt tcttttcttc aactttccta
     1501 atttctacac ctattttatc gaaaatatat ataaaactcc aaataaacac ttcgactgca
     1561 ctttttttgg caaaaaatag acagaacaaa catcctacct actaaaaata tgatatgaaa
     1621 ggaaaatgat tttttactga caccctaggc aagcttggga caaataaatc attgatagca
     1681 acatttacga catcctattt aaagttcctt caatttctaa tgtccccgct atttctaagc
     1741 cacctctggt atgatctatt cctagtcaga atagataaca ccgttctaaa ttacgattca
     1801 tagttactcc ttttttgact cgttactata agactcctat ttacatgcgc aaaataattt
     1861 tgattcagat tcatcattta tagttttgat tattttttaa cagatttttc tgttttcttc
     1921 tccgaagact ttaaaaattt ttttctaatt aaatcatcca tataaggacc aaatatacca
     1981 actactaaac caataataaa acttttaaaa tccataatta ccaccaacat gtcgctgcat
     2041 aggccacacc tccaagtata gctcctccca cagcaccagt tgctgcacct tgccatgttc
     2101 ttgttttaat tcctagctga agacctcgtg ctgctcctcc tccaacacct gctttggcaa
     2161 aatctcccca attgcatccg ccaccttcaa cgcaagcaag catctcagta tccataattt
     2221 caaattgtga catcgttttt gtattcataa cgaatacctc ttttttattt ttaatatttg
     2281 tcttgttaca aacttgacaa gtttagtata acagtatcta ttaatttttt tcatccaaat
     2341 cttgaattgg catcgaaacg tcttgaatta gcttttttgt ttcaaaatca tctctatttt
     2401 taaaaaagat gttttctaat cactttttta c
//
LOCUS       DAVLSL010000190         2382 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLSL010000190 DAVLSL010000000
VERSION     DAVLSL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.192"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            35..562
                     /locus_tag="VXN30_001982"
     CDS             35..562
                     /locus_tag="VXN30_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1358530.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            641..>2382
                     /gene="pspC"
                     /locus_tag="VXN30_001983"
     CDS             641..>2382
                     /gene="pspC"
                     /locus_tag="VXN30_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEV1358531.1"
                     /translation="MFASKNERKVHYSIRKFSIGVASVAVASLVMGSVVHATEKEVTT
                     QVPTYSNMAKTEHRKAAKQVVDEYIEKMLREIQLDRRKHTQNFAFNMKLSAIKTEYLY
                     GLKEKSEAELPSEVKAKLDAAFEQFKKDTLKLGEKVAEAEKKVAEAEKKAKAQKEEDR
                     RNYPTITYKTLDLEIAESDVEVKKAELELLKEEAKTRNKDTIKQAKAKVESKKAEATK
                     LEEIKTDREQAEATRLKKIKTDREEAEEVKDKPKKRTKRGALGEPATPDKKENDAKSS
                     YSSVGEETLPSPSLKPGKKVAEAEKKVEEAEKKAKAQKEEDRRNYPTNTYKTLDLEIA
                     ESDVKVKEAELELVKEEAKESRNEEKIKQVKAKVESKKAEATRLEKIKTDRKKAEEAK
                     RKAAEEDKVKEKAAEQPQPAPAPQPAPAPKPEKPAEQPKPEKTDDQQAEEDYARRSEE
                     EYNRLTQQQPPKAEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNS
                     NGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYL
                     NANGDMATGWLQYNG"
BASE COUNT          924 a          386 c          524 g          548 t
ORIGIN      
        1 agataaaaat atagataaaa gaaaggatac taatatggta ttagcgatta ttttagtaac
       61 attccttatt cgattgattt ttttaaagcg ttcgatagag aatgagaaac gaatccttag
      121 caatggcggg aaagaatttg gagttgagaa tacaaaacga ttaacgttgg ctcatattgt
      181 tttttatctc tcttgcctcg ttgaagcaat ggtgcataag acaatgtttg atggtatgag
      241 catggttggt ttagtcttgc ttattttttc tatgctgatg ttaatgttgg tgattcactt
      301 gttgggagat atttggacag tgaagcttat gcttgtcaat aatcacaaat atgtagatca
      361 tatcttgttt aggacagtaa aacatcctaa ttacttttta aatattcttc ctgagttgat
      421 tggcttgacc ttgttgagtc atgcttatgt gacttttgtt ttagtttttc cagtttatgc
      481 agttattttg tatcgacgaa tagctgaaga ggaaaagcta ttacatgaag ttataatccc
      541 aaatggaagc ataaagagat aaatacaaaa ttcgatttat atacagttca tattgaagtg
      601 atatagtaag gttaaagaaa aaatatagaa ggaaataaac atgtttgcat caaaaaacga
      661 aagaaaagta cattattcaa ttcgtaaatt tagtattgga gtagctagtg tagctgttgc
      721 cagtcttgtt atgggaagtg tggttcatgc gacagagaag gaggtaacta cccaagtacc
      781 cacttattct aatatggcaa agacagaaca taggaaagct gctaaacaag tcgtcgatga
      841 atatatagaa aaaatgttga gggagattca attagataga agaaaacata cccaaaattt
      901 cgccttcaac atgaagttga gcgcaattaa aacggagtat ttgtatggat taaaagagaa
      961 gtcggaagct gagttgccgt cagaagtaaa agcaaagtta gacgcagctt ttgagcagtt
     1021 taaaaaagat acattgaaac taggagaaaa ggtagcagaa gctgagaaga aggttgcaga
     1081 agctgagaaa aaagccaagg ctcaaaaaga agaagatcgc cgtaactacc caaccattac
     1141 ttacaaaacg cttgaccttg aaattgctga gtccgatgtg gaagttaaaa aagcggagct
     1201 tgaactattg aaagaggaag ctaaaactcg aaacaaggac acaattaagc aagcaaaagc
     1261 gaaagttgag agtaaaaaag ctgaggctac aaagttagaa gaaatcaaga cagatcgtga
     1321 acaagctgag gctacaaggt taaaaaaaat caagacagat cgtgaagaag cagaagaagt
     1381 taaagataaa ccaaagaagc ggacaaaacg aggagctctt ggagagccag caacacctga
     1441 taaaaaagaa aatgatgcga agtcttcata ttctagcgta ggtgaagaaa ctcttccaag
     1501 cccatccctg aaaccaggaa aaaaggtagc agaagctgag aagaaggttg aagaagctga
     1561 gaaaaaagcc aaggctcaaa aagaagaaga tcgccgtaac tacccaacca atacttacaa
     1621 aacgcttgac cttgaaattg ctgagtccga tgtgaaagtt aaagaagcgg agcttgaact
     1681 agtaaaagag gaagctaagg aatctcgaaa cgaggaaaaa attaagcaag taaaagcgaa
     1741 agttgagagt aaaaaagctg aggctacaag gttagaaaaa atcaagacag atcgtaaaaa
     1801 agcagaagaa gctaaacgaa aagcagcaga agaagataaa gttaaagaaa aagcagctga
     1861 acaaccacaa ccagcgccgg ctcctcaacc agctcccgca ccaaaaccag agaagccagc
     1921 tgaacaacca aaaccagaaa aaacagatga tcaacaagct gaagaagact atgctcgtag
     1981 atcagaagaa gaatataatc gcttgactca acagcaaccg ccaaaagcag aaaaaccagc
     2041 acaaccatct actccaaaaa caggctggaa acaagaaaac ggtatgtggt acttctacaa
     2101 tactgatggt tcaatggcga caggatggct ccaaaacaat ggctcatggt actacctcaa
     2161 cagcaatggc gctatggcga caggatggct ccaaaacaat ggttcatggt actatctaaa
     2221 cgctaatggt tcaatggcga caggatggct ccaaaacaat ggttcatggt actatctaaa
     2281 cgctaatggt tcaatggcaa caggatggct ccaaaacaat ggttcatggt actacctaaa
     2341 cgctaatggt gatatggcga caggatggct ccaatacaat gg
//
LOCUS       DAVLSL010000191         2369 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLSL010000191 DAVLSL010000000
VERSION     DAVLSL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.193"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            43..1050
                     /locus_tag="VXN30_001984"
     CDS             43..1050
                     /locus_tag="VXN30_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1358532.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1053..1253
                     /locus_tag="VXN30_001985"
     CDS             1053..1253
                     /locus_tag="VXN30_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1358533.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1405..1587
                     /gene="rpmF"
                     /locus_tag="VXN30_001986"
     CDS             1405..1587
                     /gene="rpmF"
                     /locus_tag="VXN30_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1358534.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1603..1752
                     /gene="rpmG"
                     /locus_tag="VXN30_001987"
     CDS             1603..1752
                     /gene="rpmG"
                     /locus_tag="VXN30_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1358535.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2232..>2369)
                     /locus_tag="VXN30_001988"
     CDS             complement(2232..>2369)
                     /locus_tag="VXN30_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358536.1"
                     /translation="ESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT          782 a          460 c          439 g          688 t
ORIGIN      
        1 aagtgatatc atttagatat caaataatgg gaggtcattc ttatgactga aaaactaatc
       61 aattcaaaac caaatggtgt attcgcattg attctcattg aattgacgct cgtacttggt
      121 atctttatat ttataatggg tgttggttcg gaaaacattt ttggaattat tatcggacct
      181 ttactaatcg taattgcagg tctagctcat gctggtttaa aagttgtcaa acctcaggaa
      241 gctctggttc tgacactctt tggtaattat acaggtacca tcaaagaacc tggtttttac
      301 tttgtcaatc ccttcagcgt agcagtcaac cctgcaaacc acactcgact tggacaaagt
      361 ggtgatgtta gcacaaaatc tcctttttta ggagctaaat catcaaatga caatgatgta
      421 aatcttgaaa ttggcaagaa acagatttcc ctcaaagtca tgaccttgag caattctcgt
      481 caaaaaatca atgattgctt aggaaaccct gtagaaatcg gtatcgcggt aacttggaga
      541 gttgtcgata ccgctaaggc agtcttcaac gttgataact acaaagaata tctttcactg
      601 caatgtgata gcgccctccg taatattgtc cgcatctatc cttacgatgt gtctcctaat
      661 gtggatacta cgggtgacgg gcaagcagat gaaggtagtc tccgtggctc tagcgaaatt
      721 gttgctaacc gtattcgtga agaaatccaa agtcgtgttg aggatgctgg cttggaaatc
      781 cttgaagcac gtatcactta cctagcttat gctccagaaa ttgctgccgt tatgcttcaa
      841 cgccaacaag catctgccat tattgatgca cgtaagatga ttgtagatgg tgctgtagga
      901 atggttgaaa tggcactaga acggctcaat gaaggggaat tggtagaact tgacgaagaa
      961 cgaaaagctg ccatggtttc aaatctcctt gtcgttcttt gtggcaatca tgatgcacaa
     1021 ccaattgtca acacaggaag tctttactaa agatggctga agctaaaaaa aagcagatcc
     1081 cccttcgact ctcaacaaag ttatacgctg cactcgcatc atgggcagaa gacgactttc
     1141 gttcagtcaa tggccaaatc gaatatctcc tcacagagtg tgtcaagcaa cggaagaaag
     1201 acggaaaata cgtatcagaa accattgacg aaccatttga gattgatatt taacaccttc
     1261 tcctgtctgc cgtgtcggat aggagaattt tttcatcttt ttttgtcaag taactttact
     1321 ttacaaaaaa aatgtgttat cctagtatgg ttgatgaaaa tcagtagatt gaatcgaata
     1381 taaataccta aaggagaaat caaaatggca gtacctgcac gtcgcacttc aaaagcgaag
     1441 aaaaacaaac gtcgtacaca ctacaaagta acagctccat ctgtaaactt tgacgaaact
     1501 actggagatt actcacgttc tcaccgtgta tcacttaaag gatactacaa aggacgtaaa
     1561 atcgctaaag ctgcatcagc tgaataatag aagggagata ccatgcgcgt aaatattaca
     1621 cttgaacaca aagaatctgg tgaacgcttg taccttactt ctaaaaacaa acgtaacact
     1681 ccagaccgcc ttcaattgaa gaaatactca ccaaaacttc gcaaacacgt tgtgtttaca
     1741 gaagtgaagt aagcattcaa tacgaatcaa tacagaagaa aaacgcttga tcagcgtttt
     1801 ttctttttgt taatttcact atattgcaag agaaatcatt cgaatgacta cattttttga
     1861 aataagaata cggattcgga cgattaaaag taagagaaac tcaaatgtaa gttggggttc
     1921 cttttttgta tatttttcaa ataacgcctc tacactattt gtagcaaatt caccaactac
     1981 agttgcatct tagttaaaat aagttagaat atgtaagtga gtaccagata taccaagaca
     2041 tcgtcaccat ctaaggtata ttcaaaatac aaaagttgac caactagatt tctgaatatc
     2101 cttatatatc cattcttaaa attggtttaa atagcgtagt cttttaaact agttttgaga
     2161 atccaaaaaa tcttcctaca tatgtaagaa gattttttag ttcagaatga ttagatttag
     2221 ctaatggata cctatcctac ccactcaccg ttagcattga ctcgatagcc atcgggtgta
     2281 gtcgtgttta ctaataaatc tcctgaatta taggtatagt accatttacc agaaactgta
     2341 aaccaaccag tagccattga acctgattc
//
LOCUS       DAVLSL010000192         2348 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLSL010000192 DAVLSL010000000
VERSION     DAVLSL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.194"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..357)
                     /locus_tag="VXN30_001989"
     CDS             complement(<1..357)
                     /locus_tag="VXN30_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEV1358537.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFF"
     gene            complement(475..888)
                     /locus_tag="VXN30_001990"
     CDS             complement(475..888)
                     /locus_tag="VXN30_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1358538.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(885..1121)
                     /locus_tag="VXN30_001991"
     CDS             complement(885..1121)
                     /locus_tag="VXN30_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1358539.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(1181..1774)
                     /locus_tag="VXN30_001992"
     CDS             complement(1181..1774)
                     /locus_tag="VXN30_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1358540.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSS
                     GKFLDKHDRVFEGYPAYGGNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWIS
                     DNAIYIKEFKNSDFNLVFLLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQ
                     NEFADFVALVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            complement(1740..2273)
                     /locus_tag="VXN30_001993"
     CDS             complement(1740..2273)
                     /locus_tag="VXN30_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1358541.1"
                     /translation="MKETYTDEHLEIMRDNCENLRDLAIIDLLASTGMRVGELVQLNR
                     SDIDFENRECVVFGKGKKERPVYFDARTKIHLRNYLNDRKDSHPALFVTLVGKAQRLG
                     IAGVEIRLRKLGDKLGIQKVHPHKFRRTLATKAIDKGMPIEQVQKLLGHSKIDTTLAY
                     AMVNQNNVKHSHQKFIS"
BASE COUNT          695 a          451 c          351 g          851 t
ORIGIN      
        1 aaaaaaagaa gacaatatac gcctaatatt gtctaaattt gccttactac aatccttttc
       61 agactggtaa tttgctaaat aactccttaa atcatctgtt gtgagttgtg tcacagactc
      121 tttaatagca ttaaacaagt tttcaatcgt cctctggtaa taacgaattg tttttgagct
      181 acagccctct acctgcttgg cggtaataaa tagttgcaac aggttattat tcgaatactt
      241 ctgctgcttt tttccgtcta ctagatgatg ttctaaactc tctctcaacg ctagtaattg
      301 ttcattattt aaaagaggta acatttcttg catgataatt gtaacttttg attccataat
      361 ttctcacttt cttattcatc aataaaaaga gacaatatca gtttctgcat tacagaattc
      421 taatattgtc tgttcattta ttttcttgtg ctcattcaaa tccagcatac taggttactt
      481 tttgaccttt tctctaacag aatgttcaga aacccatttg gagtagcttg tcattttttc
      541 atcagttaaa gcttctactg cgatggttac acacattttt actgcttctt ctaccgttac
      601 agaaaaacga tagccgttta attgtaaaaa tttgaccaaa acgaagaaag cagttctttt
      661 attagcatta gcaaaaacat gcttctttat caattggaca aatagtatcg ttgctttatc
      721 aaaaattgtt ggataaagag acttcccaaa gacaaattgt tctggtaagt tcacaatcat
      781 atttaaggca gaagaactaa ctgtttgaat tttctcattt ggagaatacc gttgaattgc
      841 taaagcattt attttttcaa tttgcttttc tgtcagatag attgtcattt ttccaccaaa
      901 gctttgaaaa catcgtcgta ttcgtcaaat attttatcta aatttaagtc aaaggcatca
      961 tcagatacat aagaaacctg ctgccccaat tcttcaggag taaagataat ctcaccactt
     1021 ggtaacaatt tcgcttcgta tttaacacca cttggaatat taaattcact tggaatcgta
     1081 atggtgattg aatttccttg ttttcttgtt tttactacca tctgatttcc tcctttgaag
     1141 tattataaca aatcattacc gtaattacaa tggcagaata tcagccaaaa tactcctgca
     1201 tcagagattt cttcaaagtt tcaagttctt ccagagattt ttggattgcc aattgtgatt
     1261 tgtcgaccag ggctacaaag tctgcgaact cattttggag ggctagaggg gggaggatat
     1321 atttctgatt ttctaatggt ttttgagtta tctttggttg acctgagaaa tcagcaaatt
     1381 tactaaaatc aattactttc attaattcta ataaaaatac aagattgaaa tcggaattct
     1441 taaattcttt tatatatata gcattgtcac ttatccatac cttaccgtgg gtagttctta
     1501 cattaccaca atatgcacct acacgaccaa tgatgatagt aggattatca attaaatatt
     1561 ttcgactttt ccaagcaatg ccatttccac cataggcagg ataaccttca aaaactctat
     1621 catgtttatc aagaaatttt ccacttgaaa atttaaaaca ctgttgtccc gtttttactg
     1681 caaatttctt gttattattt aaaggatccc caaacatctc gttaaatcgg gatttgcttt
     1741 taagagatga atttttggtg tgaatgcttg acattatttt gattgaccat ggcataggcc
     1801 agggttgtgt caatcttgct atgacctagc agtttttgga cttgttcgat aggcatacct
     1861 ttatcaattg ccttagtcgc taaagttctt ctgaacttat gtgggtgaac cttttgtatg
     1921 ccgagtttgt ctcctaactt tcttaagcga atctctacac cagcaattcc aagtctctgg
     1981 gcttttccaa ctagcgttac aaaaagagca gggtgactat cttttctgtc gttaagataa
     2041 tttcttaaat gaattttcgt ccgagcgtca aaatatactg gtctctcctt ctttccttta
     2101 ccaaagacaa cacactctct gttttcaaaa tcaatatctg aacgattcaa ctgtacaagc
     2161 tcccctacac gcatacctgt cgatgctagt aggtctatta ttgccaaatc tctcaaattt
     2221 tcacagttat cacgcataat ttccaaatgt tcatcagtat aagtttcctt cacattttgc
     2281 tcagtcttaa ttttctttat ccgtcgaatg ggatttttaa tgatatattc ctcttgctca
     2341 agccaagc
//
LOCUS       DAVLSL010000193         2283 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLSL010000193 DAVLSL010000000
VERSION     DAVLSL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.195"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            148..897
                     /gene="truA"
                     /locus_tag="VXN30_001994"
     CDS             148..897
                     /gene="truA"
                     /locus_tag="VXN30_001994"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1358542.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            887..1660
                     /locus_tag="VXN30_001995"
     CDS             887..1660
                     /locus_tag="VXN30_001995"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1358543.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            1638..2099
                     /locus_tag="VXN30_001996"
     CDS             1638..2099
                     /locus_tag="VXN30_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1358544.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT          638 a          439 c          521 g          685 t
ORIGIN      
        1 gcaggttgct caaaatagtg ttttgaggtt gcagatggaa actgacgtgg tttgaagaga
       61 ttttcgaaga gtatgagatt tttataccca tacaatattt gttttaggtc cttgtttaca
      121 gactaatttc ttgataaaat agaggttatg acgagatata aagcaactat ttcctatgat
      181 ggttatgcct ttgctggctt tcagcgccag tctcatgcgc gtagcgttca ggaagaaatt
      241 gaaaaaacct tgaccagatt aaataaaggg caaaccatta ctgttcacgg tgctggtagg
      301 acagatagtg gggttcatgc cctgggacag gtcattcatt ttgacctgcc ttatcagatg
      361 gatgaggaga aactccgttt tgccttggat acccagtctc ctgaagatat tgatgtgatt
      421 tcgattgagc ttgtggcaga tgattttcat tgccgttatg ccaaacatag caagacctat
      481 gagtttattg tggatagagg acgtcccaaa aatcctatgc gccgtcacta tgccacccac
      541 tttccctacc cactcgatgt ggaacgaatg cagattgcaa tcaaaaagct agagggaacc
      601 catgatttta ccggttttac agcctctggg actagtgtag aggataaggt tcgcaccatc
      661 acagaagcta gtttaatagt cgatgagaca ggacaatttt tgacctttac cttttcagga
      721 aatggtttct tgtataaaca gattcgcaat atggtgggga ctcttctcaa aatcggtaac
      781 aaccgcatgc cagtagagca gattgatctc atcttggaga agaaggacag gcaacttgca
      841 ggtcccactg cagcaccaaa tggtttgtat ttaaaggaga ttcgttatga agaataatcg
      901 tattttagca ctttctggaa atgatatttt tagtggtggt ggactgtcag ctgatttggc
      961 tacctatacc ttgaacggct tgcatgggtt tgtagcagtg acttgtttga cagccttgac
     1021 agaaaaagga tttgaagtct ttccaactga tgataccatt tttcaacatg aattagatag
     1081 cttacgtgat gtggaatttg ggggaattaa gattggtctt ctccctactg tcagtgtggc
     1141 tgagaaggcc ttggacttta tcaaacaacg cccaggagta cctgtggtgt tggatcctgt
     1201 cttggtctgc aaggaaacgc atgatgtagc tgtcagtgag ctctgccaag agttgattcg
     1261 ctttttccct tatgtcagtg tgattacgcc taatctccca gaagcagaat tattatccgg
     1321 tcaggaaatt aaaaccttgg aagacatgaa aactgcagcg cagaaattgc atgatttagg
     1381 agcgccagca gtcattatca agggaggcaa tcgtcttagt caggacaagg ctgtggatgt
     1441 cttttatgat ggacagacct ttactatcct agaaaatcca gttatccaag gccaaaatgc
     1501 tggtgcaggt tgtacctttg cctctagcat tgccagtcac ttgattaaag gtgataaact
     1561 tttgccagca gtagaaagct ctaaggcttt cgtttatcgt gctattgcac aagcagatca
     1621 gtatggagta agacaatatg aagcaaacaa aaacaactaa aatcgccctt gtatccctat
     1681 taaccgccct ttctgtggtt ctaggttatt tcttaaaaat cccaacacct acaggaattc
     1741 taactctttt agatgctggt gtcttctttg cggcctttta ctttggtagt cgtgaaggag
     1801 cggtagtcgg aggactagca agtttcttga ttgacctctt atcaggctac cctcagtgga
     1861 tgttctttag cttggtcaac catggcttgc agggattttt cgcaggattt aaaggaaaaa
     1921 gtcagtggtt aggccttatt ttagcaacta ttgccatggt aggaggctac gccttgggtt
     1981 ctactttgat gaatggctgg gcagcagccc tcccagaaat tctaccaaat ttcatgcaaa
     2041 atatggtagg gatgattgta ggatttattc ttagtcaaag tatcaagaag attaagtaat
     2101 actcttcgaa aatctcttca aaccacgtca gcttccatct gcaacctcaa aacactgttt
     2161 tgagcagcct gaggctagct ttctagtttg cactttgatt ttcattgagt ataaagaggt
     2221 tgagtaaaaa gtatttcaaa taagaaaaac gcatagtatc aggtgttgaa tgaactaccc
     2281 ccc
//
LOCUS       DAVLSL010000194         2216 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVLSL010000194 DAVLSL010000000
VERSION     DAVLSL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.196"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..894)
                     /locus_tag="VXN30_001997"
     CDS             complement(<1..894)
                     /locus_tag="VXN30_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1358545.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(897..2159)
                     /locus_tag="VXN30_001998"
     CDS             complement(897..2159)
                     /locus_tag="VXN30_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1358546.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
BASE COUNT          766 a          502 c          301 g          647 t
ORIGIN      
        1 cctatttact gacctagcca agcgagatac tgtatgtctc actcctctaa ttgccttgct
       61 caatgcataa atattatcga ctgttgccag tcccccatat cctgcgtcac ctatatgttg
      121 aatatcaact ccacaaattt tatttctaag tgcaatttcc ttaatagtat ctgtatcaga
      181 tgtttcttgg ctagtaccaa tagcactcaa tacaagacca cccttactat gaacgagatc
      241 aacgacttca cgcaactctt ggtcatgaaa ggctggaacg gttccaactg ctggaacaag
      301 tatcacatcc gcacctgctt ccaacaattg ctctgcgaca gaaagctctg ccacaggctc
      361 attcactcct gccccgtgca tcttacctgc aataatcaaa ccagaaaagt tttccttagc
      421 agtttgaaca gccttaatga tttctcgatt gctaactcct gttccaggat ttccagtcaa
      481 acagacaaag tcaaaaccta actcctctat tcgcttcaat gtttcaacac tagcaacacg
      541 acctgcaaca atttcctgtg tttcctctag catctttgca gatgggtcaa tcggttccaa
      601 atttacacca attggacaag caacaagctg gtgtaagcga tgaataacat catcacctga
      661 actatccaaa gcataaattt taggctcaaa aacatcaaca caattcaata aaatcatatc
      721 agctccaaaa gctctagcaa tttcagaatt agttatatcc ccaacaaatg tctcacgagt
      781 aactacattt tcagatagaa caactctacc ttcacttgct aaaatacttt gttttaattc
      841 ttcagcattc atctgtaata tctcagatgg atttgcacta attaaacgtt tcattctcaa
      901 tcctccaata acttaaaccg tttttcaata gctattacaa atggtaaata aagtaagaca
      961 tccaaaacaa tcaatacaat ttgtaataat gaaccagaaa tacttcctgt agcaagtaaa
     1021 ccacttataa caggtggcat agtccaacct ggatctgtat aagttagcgg aaccaaacct
     1081 gatgccattg ctccccacgc tactagtaag ttaaacattg gagctaagat aaatggaact
     1141 agtaacaaaa tgttcaaaac aataggaacg ccaaacattg ctggttcatt tatattaaat
     1201 aatccaggta taacagttat aggtgccaaa gtttttagtt gtttactagc tttcttctta
     1261 tgtgctaaat atcctaacgc aagaactaaa cctatggttg caccaccgcc acctatgaat
     1321 acaaaattat ccataaatac gctagtaaaa atatttggaa gagtttctcc agtttgataa
     1381 gctactttat ttgcatctag attagctaac cataacggtt tcataactgc attaacaaca
     1441 tttcctccgt gtaatcctac aaaccaaaaa ctactattaa ggaaaattaa gataagaagg
     1501 ccaataacat tatttcctaa aagtccaagt ggacctccca aagctacttg agcaatttca
     1561 tgaagattag gtaaacttaa tttatctaaa gttgcataaa caatcaacca accaacaata
     1621 gtcactgcac caggaataat ggcactaaag cttttagata ctgctggtgg aacaccatcc
     1681 ggcattttta tctgtatatt atgatttata aaccattggt agatatagcc attaatcaat
     1741 cctaaaatca tagcaacaaa taaacctttt gatgccatga aagcggtagg cattcctgct
     1801 ccagcctctc ctctaatgaa aggcgtaact gtaataaaag atgataatga cacaatacca
     1861 gcaggaatac catcaacttt gtattgtcta gccataaagt aagcgatacc aaaactagct
     1921 acaagaccaa gtaatccaaa actactatca acacccttcc ataaatagtc agcaacacca
     1981 atatcaccca gatatttttc ccatcctgga ataggaaaac ttgcaataac cattagtaat
     2041 gaacctatca acaataaagg tatagttaga gtaataccgt ctcgaataga aaccaacgct
     2101 ttattattac ccaatctagc cgctactgga agtaactttt cttgtaataa tttttccata
     2161 accttttcct cttaaatata ttttattatc aaaagttatc aattatcttt attttt
//
LOCUS       DAVLSL010000195         2211 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVLSL010000195 DAVLSL010000000
VERSION     DAVLSL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.197"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..978
                     /locus_tag="VXN30_001999"
     CDS             <1..978
                     /locus_tag="VXN30_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1358547.1"
                     /translation="SKKILLLTVTKNMEKVLDKLLESDELSWKLVAVSVLDKSDFQHD
                     KIPVIEKEKIIEFATHEVVDEVFINLPGESYDIGEIISRFETMGIDVTVNLNAFDKNL
                     GRNKQIHEMVGLNVVTFSTNFYKTSHLISKRILDICGATIGLILFAIASLVLVPLIRK
                     DGGPAIFAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQLMDQNTMQGGMFKINNDPRVT
                     KIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPKQKRRLSFKPGITGL
                     WQVSGRSKITDFDAVVKLDVAYIDNWTIWKDIEILLKTVKVVFMRDGAK"
     gene            1009..2190
                     /locus_tag="VXN30_002000"
     CDS             1009..2190
                     /locus_tag="VXN30_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV1358548.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKAIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIAYDIAAINRAIEIAKENKDENPIF
                     YILACRIGPFIHGIKKRIQAIGGTLLVNPDGHEWLREKWSLPVRKYWKYSERLMVKYA
                     DLLVCDSKNIEQYIQEDYKQFQPKTTYIAYGTDTTPSILKFEDTKVRNWYQEKGISEN
                     GYYLVVGRFVPENNYEAMIREFIKSQSKKDFVLITNVEQNKFYDKLLRETGFDQDPRI
                     KFVGTVYDQELLKYIRENAFAYLHGHEVGGTNPSLLEALGSTKLNLLLDVGFNREVGE
                     DGAIYWQKDELSRVIEYVETINQATIANLNFKSTQRILSDFTWEKIVADYEGVFCFAK
                     S"
BASE COUNT          738 a          311 c          466 g          696 t
ORIGIN      
        1 agcaagaaaa ttttactgtt aacagtaacg aaaaatatgg aaaaagttct tgataaattg
       61 ctagaatctg atgaactttc atggaaattg gtagcagtaa gtgttttgga taaatctgat
      121 tttcaacatg ataaaatacc tgtaattgaa aaggaaaaaa ttattgaatt tgcaacgcat
      181 gaagttgtgg atgaggtgtt tatcaatctt ccaggagaga gctacgatat tggagaaatt
      241 atctctaggt ttgagacaat ggggatagat gtaactgtaa atcttaatgc atttgataag
      301 aatttgggtc gcaataaaca aattcatgag atggtaggat tgaatgtagt cactttctct
      361 acaaattttt ataaaactag tcatttgatt tcaaagagaa ttctcgatat ttgtggtgcc
      421 actattggcc ttattctttt tgctatagct agtctagttt tagttccatt gattcgtaaa
      481 gatggcggac cagctatttt tgctcaaact cgtataggga aaaatggtcg acattttacc
      541 ttttataaat tccgttcaat gcggatcgat gctgaagcta tcaaagaaca gttgatggat
      601 caaaatacga tgcaaggtgg tatgtttaag ataaacaatg atcctcgtgt tacaaaaatt
      661 ggtcgcttta ttcgtaaaac cagtttggat gaattgccac aattttggaa tgtctttatt
      721 ggagatatga gtttggttgg gacgcgtcca ccgacagtag acgaatatga tcagtatact
      781 ccaaaacaga aacgtcgact cagctttaag cctggtatta caggcttatg gcaggttagt
      841 gggcgtagta aaataaccga ttttgatgct gttgtaaaat tagatgtggc ttatattgat
      901 aattggacaa tctggaaaga tattgaaatt ttgcttaaaa ctgttaaagt tgtatttatg
      961 agagatggag cgaagtaatt tctgtatatc catcatatta ggagagaaat gaaaaagtca
     1021 gtttatatca ttggttcaaa aggaattcct gctaagtatg gaggatttga aacttttgtt
     1081 gaaaaattaa cagccttcca acaagataag gctatccaat attatgtggc ttgtatgcgt
     1141 gaaaactctg caaaatcagg gactactgag gatgtttttg aacataatgg tgctatctgt
     1201 tataacgtcg atgttcctaa tattggtcca gcgcgagcta tagcgtatga tatcgctgca
     1261 attaacagag ctattgaaat tgccaaagaa aataaggatg aaaatccaat cttctatatt
     1321 ttagcttgtc gaattggtcc gttcatccat ggaattaaga aaagaattca agcaataggt
     1381 gggactcttc tggttaatcc ggatggtcat gagtggctac gagaaaaatg gagtttacct
     1441 gttcgcaaat attggaaata ctctgaaaga cttatggtca aatatgcaga tttattggtt
     1501 tgtgacagta aaaatatcga acagtatatc caagaagatt ataaacagtt ccaacctaag
     1561 accacctata tcgcttatgg gaccgataca actccctcaa tcttgaaatt tgaagatact
     1621 aaagtccgaa attggtatca agagaaggga attagtgaaa acggttatta tttagtggtg
     1681 ggacgatttg tccctgagaa taactatgaa gctatgattc gtgaatttat caaatcccag
     1741 tctaaaaagg attttgtcct cattacaaat gtggaacaaa ataaatttta cgacaagttg
     1801 cttcgggaaa caggttttga ccaagaccca agaatcaaat ttgttggtac tgtttatgat
     1861 caggaattgt taaaatatat tcgagaaaat gcctttgcat atttacatgg tcatgaggtg
     1921 ggggggacaa atccatcact tttagaggct ttaggatcaa caaagttaaa tttgttgctt
     1981 gatgttggtt ttaaccgtga agttggtgag gatggagcta tttattggca aaaagatgaa
     2041 ctttcacgag tcatcgaata tgtggaaacg ataaatcaag caactatcgc aaacttaaat
     2101 tttaaatcaa cccaaagaat cctctcagat tttacatggg aaaaaattgt ggcagattat
     2161 gaaggagtat tttgttttgc gaagagttag tgtgcccaat ttatatgcag t
//
LOCUS       DAVLSL010000196         2143 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVLSL010000196 DAVLSL010000000
VERSION     DAVLSL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.198"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            129..1622
                     /locus_tag="VXN30_002001"
     CDS             129..1622
                     /locus_tag="VXN30_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1358549.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKVISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>2143
                     /locus_tag="VXN30_002002"
     CDS             1635..>2143
                     /locus_tag="VXN30_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1358550.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLLV"
BASE COUNT          755 a          321 c          426 g          641 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg taatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaagggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cgt
//
LOCUS       DAVLSL010000197         2133 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVLSL010000197 DAVLSL010000000
VERSION     DAVLSL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.199"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            88..1611
                     /locus_tag="VXN30_002003"
     CDS             88..1611
                     /locus_tag="VXN30_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358551.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1577..>2133
                     /locus_tag="VXN30_002004"
     CDS             1577..>2133
                     /locus_tag="VXN30_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358552.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPSILSFIFIPLSKKY"
BASE COUNT          745 a          297 c          401 g          690 t
ORIGIN      
        1 aattcaaact tatccggtgc agagagtata gcattaaaat ctgttattac actaacaagt
       61 ggagcgatat tttattttgt ctcaggaatg ttttcacata tcttgggatt taggcttgaa
      121 acaaatttaa gaaaaagggg aatcgatggt ctggaaaaag caagttttag gttctttgac
      181 ttaaatccat ctggtcaaat aagaaagatc atagatgata atgcagcaca aactcatcag
      241 gtggtagcac acatgattcc cgatagttct caggcaataa tcacacccgt acttgtactt
      301 gcacttggct ttatagtaag tataagggtt ggcataattt tgcttgctct tactataatt
      361 ggtggcttaa ttttaggggc aatgatgggc gagcaagaat ttatgaagat ataccaagaa
      421 tccctatcta aactaagtgc tgaaactgtt gagtacgtga gaggaatgca agttgtaaaa
      481 atatttaaag caaatgtaga gtcttttaaa agcttttata aggcgataaa agattactca
      541 aagtatgctt atgattattc cctatcttgt aaaaggcctt atgttttgta tcaatggtta
      601 ttttttggac tgattgcaat tttaattatt cctatagttt attttatgac tagcttagct
      661 agcgcaaagg tgattttact tgagcttatc atgattttat ttttatcagg agttctcttt
      721 gtttcattca tgagaatgat gtggtactcc atgtatattt ctcaaggaaa ttatgcagta
      781 gatactttag aggcgcttta cgaagatatg caaaaagaca aattagtgca tggtaatgtc
      841 aataatttta aaaactataa tatagaattt gagaatgtta gctttgctta taatgataaa
      901 gctgtcattg aaaatttatc ctttaattta gaagaaggaa agtcctacgc acttgtcggt
      961 tcatctggat caggcaaatc aacagtagca aaacttatat caggttttta caatgttaat
     1021 aaaggaagca taaagatagg cgggatagca ataagtgaat attctgacga agccttaatt
     1081 aaagccattt cctttgtttt tcaagattca aaattattca agaagagcat ttatgataat
     1141 gtagctttag ctaataaaga tgcgacgaaa gatgacgtta tgagagcctt aaaattagca
     1201 ggatgcgatt taatattaga caaattccca gaaagagaaa atacaatcat aggctcaaaa
     1261 ggtgtttatt tatccggtgg agaaaaacaa agaattgcaa ttgctagagc aattttaaag
     1321 gattccaaaa ttattattat ggatgaagca tcagcatcta ttgacccaga taacgagttt
     1381 gaattgcaaa aagcttttaa aaatcttatg aaggataaaa cagttatcat gattgcacac
     1441 aggctatcta caattaaaga ccttgatgaa attattgtca tggatagtgg aaaaattata
     1501 gaaagagggt ctgacaaaga attaatgtca aaagatacaa ggtataagag cctgcaagag
     1561 atgtttaaca gtgcgaatga atggagggtt tcaaatgaaa gagttttata aaaaaagatt
     1621 tgctcttaca gatggaggag caagaaattt aagtaaagca acactggctt catttttcgt
     1681 ttattgtata aacatgcttc ctgccatatt acttatgatt tttgctcagg aagttttgga
     1741 aaatatgggc aaaagcaatg gcttttatat agtattctca gttttgactt tgatagcaat
     1801 gtatattttg ctttctatcg aatacgataa attatataac acaacctatc aagaaagtgc
     1861 agatttaaga ataaggacag cggagaattt atcaaaatta cctctatctt acttttctaa
     1921 acatgacatt tccgacattt cacaaacaat catggctgat attgaaggca tagagcatgc
     1981 aatgagccac tcaataccaa aggtgggcgg catggtactg tttttcccat taatatctgt
     2041 aatgatgcta gcgggcaatg tcaagatggg tttagctgta attattccat ctattttaag
     2101 ctttatattt atacctttat ctaaaaaata tca
//
LOCUS       DAVLSL010000198         2132 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVLSL010000198 DAVLSL010000000
VERSION     DAVLSL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.200"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            166..315
                     /locus_tag="VXN30_002005"
     CDS             166..315
                     /locus_tag="VXN30_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358553.1"
                     /translation="MRFIVGRFTSFSLEIEFSPTSKLDDLLFKIAFLMILATWIKARK
                     TKGAT"
     gene            complement(244..555)
                     /locus_tag="VXN30_002006"
     CDS             complement(244..555)
                     /locus_tag="VXN30_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358554.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(570..1856)
                     /locus_tag="VXN30_002007"
     CDS             complement(570..1856)
                     /locus_tag="VXN30_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1358555.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQAGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          644 a          459 c          375 g          654 t
ORIGIN      
        1 agacggattg ttttgacaat tctaatcttt tagactttgc ataataactg ttaaacagtc
       61 gaaaattacc ccagttttaa caagtagtca aaataccaga taaaggaaaa aagtctttaa
      121 aatcttaaaa acgcatagta tcaggtattg aacaatcttg atattatgcg ttttattgtg
      181 ggaagattta cttcattttc tcttgaaatt gagttttctc ctacttcaaa actagatgat
      241 ttgctattta aaatagcgtt ccttatgatt ttggcaacct ggattaaagc gagaaaaaca
      301 aaaggggcaa cttaaacttt ctttatattg cttatatgtt agtagtttta gacaaacacc
      361 acataaaata ggcttatcct gtatcaaaga taagggatac ggctcaaaag ggtgattttc
      421 taatgaatca tgacaccgat aacaagcata atacttttta caatcatagc attgtaaagc
      481 aataatatcc agcttgctat ggtaatgaat gcatcttgtt tgcttatcca ctaagaaacc
      541 ctgtgcctct atcatttcct tttcctttct taagctcgaa ctgtgacgct ggttccatct
      601 tccttataaa aattgatgag tccttctttt aatgcacgaa ccatgtctcc agcttgaacg
      661 ggttgtggga ctttaatagt caatagttcc attggatttg gagcgcggtc gattttattg
      721 cctttggcat catgcaaatc ttcaatatag gtttcaaaat gacggaaacc tggaccataa
      781 aactcaactt ggtccccttc gttaatgacg tttcgttgac gaatggttgc tgtttgtgtc
      841 gcatcatcat aagaaaccac ttcagcgaca aacttgtact cagggatttt acggcgagca
      901 ccaaacaact gctcattttc agatggtata ccgtagtaga aacctgttgc caattcacgt
      961 tgggcaacct tccacatctc gtccactaag tcttgtttga tagcttcaaa cttttcagga
     1021 ctttcaagat aggcatccac agccgccttg tagcagttgg ttactgttga tacgtagtga
     1081 atagacttca tacgtccttc gatttttaga ctgtccacac cattttcaat catatctgga
     1141 atgtggtcaa tcatagacat gtcaacggct gacattgaaa attcttctgg aatctcaccc
     1201 tgcaaactct tacgttcttt cccaaatggc atatcgtaaa ggtcgtattt ccaacggcat
     1261 gactgagaac atccaccacg gttggcatca cgcatactca tgtggtttga aagagtacaa
     1321 cgtccagagt atgaaataca catagctcca tggacaaagg cttcaatttc aacatctgta
     1381 cgtttgcgga tctcagctaa ttcttccatt gaaacctcac gcgctaaaac gacacgagtc
     1441 aagcctagct ctttccagaa ctcaagggtt tcatagttag tggcactggc ttgggtagaa
     1501 aggtggattt caaggcctgg tgcttcagtc gctgcaatca taatcaaggc tgggtcagat
     1561 acgataactg ctgcaatccc gatatcacgc agtttacgga accactcacc agcaccagct
     1621 tcatttcctt cgtgcataac catattagcc gctacataga ccttggcacc atacttggcc
     1681 gcaaactgca cgccttcttc catctgttcg aaagtaaagt ttcccgcacg gctacgaaga
     1741 ccataggcct gaccaccgat aaagacagca tctgctccat actgaacagc tacctttagc
     1801 ttctctaaag tccctgcagg tgataaaacc tcaggacgtt ttaatgtttt tgtcatgatt
     1861 tctcctattt gcaatataaa agtttgacat ttttccttct cattttatag taaataaagg
     1921 ataaaatcaa gcctagacgg attgttttga caattctaat cttttagact ttgcataata
     1981 actgttaaac agtcgaaaat tatcccagtt ttaacaagta gtcaaaaagt ctagaaaagt
     2041 aaaaaccagt atccaaagat actgactcgt aaaaatacat aaattagtcc ttagatgact
     2101 actcccactc tgttgctcct ataatggaaa ag
//
LOCUS       DAVLSL010000199         2109 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVLSL010000199 DAVLSL010000000
VERSION     DAVLSL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.201"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            241..615
                     /locus_tag="VXN30_002008"
     CDS             241..615
                     /locus_tag="VXN30_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1358556.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            823..1725
                     /locus_tag="VXN30_002009"
     CDS             823..1725
                     /locus_tag="VXN30_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358557.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPLDFVVSTSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEMSISNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            1781..1849
                     /locus_tag="VXN30_002010"
                     /pseudo
     CDS             1781..1849
                     /locus_tag="VXN30_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0227 family bacteriocin-like peptide"
BASE COUNT          700 a          271 c          408 g          730 t
ORIGIN      
        1 ttactaagtc aaaaaaaata aattttgaaa tagtattagt gagttcgcaa tcatttattg
       61 ctagtggttt agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta
      121 taaaaacaaa taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca
      181 gtcgtctatc acttgttagg aaacgatcct aactatgctt aataaaaaag gagaaaataa
      241 atggaactcg tattaccaaa taattatgtt gctcttgagc aagaagagat gatgtatctt
      301 gatgggggat tttctattct gagatggcct gttgcaacag ccattaatat agcttttaat
      361 ggtgttttag gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg
      421 cggcgagtta caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca
      481 aatagagtgg caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt
      541 ggcgatgcta ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta
      601 aacgccttgt ggtaactaga tatgggtatt aaaatggaaa aaatttttgt tattattttt
      661 tttgtatgtc tatttatatc atccataact tttttagcct atgattttgt tagcgaagaa
      721 ataaaaaagt tgattatttg gatgaatgtc gtttttttga ttttaatcat agcaatgata
      781 atttatccaa aattaagaaa atgataagga cactgtaaaa ttatgatacg taaactaatc
      841 atttttttat taatgctccc tctttttgtc ttgtggctag agttgcattt gttagtgagt
      901 aacttgcaat tgaatctaga aattcccctt gattttgtgg ttagtacgag tcttgctttc
      961 tgtagtttga ttttatcaaa aatagtttta gacatactat acgcattgaa agatttgtac
     1021 aagaaagaag ctcttattac gatttttcct tttattttta taggcaggaa gaaagtaaat
     1081 gtgaggttct cgccctattt ttcattccat cgtaaaagtt tgtcgcctga tgatttaaga
     1141 tctaggatta tatggagttt tattcttgaa attgctatta ttctagtatt tattttaaaa
     1201 attccttttg caatcatcat gcttactaca atatttttct ggactataat ggatatcaat
     1261 catctcgttt ttaataagac ggaatttctt tttaatcaaa ataaatggga aaaagaagat
     1321 tcgtttgaaa gcgatttgac gaaaacgtta aaggataaga tacaaaaatc agagctaagt
     1381 tattctgact taatgtctct tcagctgtat gatgcgatga accaatccac ctttttaacg
     1441 gatagtgaac tttttgagga tattttgaaa aaaatcgaag attctcataa cacccttctg
     1501 tgtacaggtc ttgtcgagtt actgctgtat gaaatgtcta ttagtaacaa taacaactgg
     1561 tccaacaagg ttgataaaat taggatacac ctcatcagaa tcaatcaatt agattttttc
     1621 tattatacaa gttggttaag gcaaaatttt gatttctgta tgaatagaga gtatcataaa
     1681 atgaaatctc ggaaattatt actgagtaat aaaaagatag tttaagttcg tcctatttgt
     1741 aaaacaaatg aaacaatttc aactaaggag gagaaaacaa atggaacttg tattaccaaa
     1801 taattatgtt gtgattgatg aagaagaaat gatgtatctt gatgggggag cttatttaag
     1861 caagcgtgct tgtcaaggaa tttgcgtagc tttagctatg agttcaggaa cttttatagc
     1921 attagctgga gctgcagttt taaccaaaaa actaataaac tatattaagg ttggaggtct
     1981 tggaggctgg cttattggtg cagcagcagg aaaaatagct tactgtattg gatatggtgc
     2041 tcttaataga ggttgtgata ttagcgggaa cccttatcct tgggatggat ttatatctgc
     2101 gacagtaag
//
LOCUS       DAVLSL010000200         2022 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVLSL010000200 DAVLSL010000000
VERSION     DAVLSL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.202"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            71..802
                     /locus_tag="VXN30_002011"
     CDS             71..802
                     /locus_tag="VXN30_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358558.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHASYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            817..1968
                     /locus_tag="VXN30_002012"
     CDS             817..1968
                     /locus_tag="VXN30_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1358559.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDNYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
BASE COUNT          736 a          257 c          383 g          646 t
ORIGIN      
        1 ctaattattg tagtaatttc tttaagaaaa tgatataatt cattaaaaag tatttggagg
       61 aggaagaatt atgttgaatc aggatctctt tgattcgctt gaggcacaaa aaattgtaga
      121 tactttgatg aaagggcaaa aagattatgt agatgaacgt ctagaaaaaa gagagacaat
      181 gatagtgtcg aatggttatg catggacgcg acctaatcat attgatactg catttgcatc
      241 agcagatttg tttgagtata aattacaatt agcaggacag acttggggat atttagaatt
      301 tgaaacaaat acagaaaaat atgggaaagt attgttaatt ataaagggta agaagcgact
      361 tacgaaccaa tttcctttgg tacaaaaaaa taagagtggc tacttatttg aatatgctca
      421 gatgaataca ctttatctta atcaacatgc ttcctacaaa aatgatgaag atagtcattc
      481 ctttccaatt cagatggagt tagtttctga tgaaatgatt caagaaattg aacaagctac
      541 taaaaattcg aatatcgaaa aatttatgat tttaacttat gaggcggact cagaaaacaa
      601 tattatatct gtagatgttg ttatgcctga tgcacgaact ggtcagttac acttgattca
      661 agatttgtct gagtatattc aatcaagttc gtaccatttc gaagaagcta aatatcaaga
      721 tattcctaat ttttcagaat tatcggaaac agaagatttt gaaattattc caagaataga
      781 aaaacaagaa ggtcaaaagt agtaaggagt atgtaaatgt ttaatggtcg ggtattgaaa
      841 gaattacggc tgttaaatgg tttaagtaga gcagaattag ctcagagaat taatttaacg
      901 gaacaagcca tttggcagtt tgagtccaac gaaacgaaac ctaaattatc aaccaaaatg
      961 catttggcca accaatttca tgttgattta acttattttg aacaggaaga agagagcatt
     1021 cgatttgatt cttctgtaat tgcctttaga aatgcagacc tagcaacacg gaaaacaata
     1081 gatattcaaa ctatgtattt acataaggta gatagtttga ttgattattt tgaaagtttt
     1141 gtaattatac ctaatattac aattcatgac ctaagtaatg tagtgagtga atcttatcat
     1201 aagggagaat ccattgagga attggctctt tatgccaggg aaaaattagg tatttcaaaa
     1261 gataatcatg atttgcttta taaattagaa cgttcaggca tctatatcgt ggaacgatta
     1321 attaatggcc aagctgatgc ttatagcgca tggtcaaaat tgggaagacc ttatattgtg
     1381 ttgggaacga ataaatcatc tgtacgtcga aattttgact tagctcatga gctaggacat
     1441 attcttttac ataaatataa agatatgaat gaagatggcg atcgtttgga gcaagaagca
     1501 aattattttg catcatgttt tttattgcca aaagaagagt ttttagtcaa atttgaagag
     1561 agggttggca agcgtgtcag caatcctgat aattatattt tattgaagtc ggatttgaat
     1621 gtttcgatac aggctttaga gtatcgagct tttaagttag gattattgac tccaaagcaa
     1681 cattcttact tttatcgtca aattgcgcaa aaaggttaca aaatgattga acctttggat
     1741 gatcaaattt ttgttaaaaa accaagcaaa gtaaagagta ttctggacgt cgttttgagt
     1801 aatcatctag tcagtctagc gactataatg tctaaacaaa gtattcgttt acagtttata
     1861 agcgaaatat tttcagtcga aatgaaattt tttgatcagt atcaagaaga tagaagaaca
     1921 gatcgatttg ataacatcat ccctttgtac aaaagaaata atttataatt taactgtgaa
     1981 ataatttcat gaaggatata tggcagaaat tggattttat ca
//
LOCUS       DAVLSL010000201         1977 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVLSL010000201 DAVLSL010000000
VERSION     DAVLSL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.203"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..243)
                     /locus_tag="VXN30_002013"
     CDS             complement(<1..243)
                     /locus_tag="VXN30_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358560.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVD"
     gene            complement(257..970)
                     /locus_tag="VXN30_002014"
     CDS             complement(257..970)
                     /locus_tag="VXN30_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1358561.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1428..1808)
                     /locus_tag="VXN30_002015"
     CDS             complement(1428..1808)
                     /locus_tag="VXN30_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1358562.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
BASE COUNT          655 a          395 c          305 g          622 t
ORIGIN      
        1 atctacttgt gaatcccttc tcccaatata atccccttct ttgactaatg atggagattt
       61 ccaagtaaaa attaaatcgc cagagaaatt tttcttatca aaaaacttaa aaagagattg
      121 ataaatacta tttgctaatt gacaagtgtc agatgtaaat tcataattaa cagcttcatt
      181 aaaatcaact tcagacattt caatgtactc ttcaatagta atattagctt tttttgtagt
      241 cattagctaa tctcctttaa aatgtgatct tttcaagaat atttttatct aaacaatcca
      301 gcaagtcttg gtaagaatag acttcgtaag tcggctgggc ttgtgtgtga ttttcgaggt
      361 gatgaggatt ataccagata gtgtcaatcc ccgcattatt gccaccttga atgtcggcgg
      421 ttagagaatc tccaatcatc agcgtctttt ctttactaaa tccagcaatt tgctggccaa
      481 tcttttcata aaagagagca tccggctttt gagtttgcaa ctgttctgag ataaagactt
      541 gattgaaata aggtactaga ccagattgag ccaaacgtcc tgtctgaatg gcagtaatgc
      601 catttgtcgc agcatacaag tcataatcac gctcaatgag gctgtccaag agatcatgag
      661 cgcccgatag tgtttgtccc tgctgagcga gataaaattg gtaacgctgg gcaagaaaac
      721 taccgtcttt ttcctgtcca aaatgagaaa ataaacgaga aaagcgcgtg ttaaccaact
      781 cttgtttact gattttcttc agctccaagt ctttccagag agccttgttc ataggaacgt
      841 aataatcttt ataagcctga atatccgcaa ctccttcttc ttttagaagt tgggtcaaag
      901 ccacatcctc agcagcatca aaatcaagaa gagtgtggtc gaggtcgaag agtagaaatt
      961 tgtagaacaa tttgaggttt tcctttctga aaattcatta agaacattat atcataaagc
     1021 acctcataca attaactaat ttaatcactt aaaaaaattc gaacactttc tatacaactg
     1081 acagctcaaa tctttcagaa tagaacaata ctaactatcg aacaccccgt cttcataaat
     1141 acatatgtaa ttctagacct agaattccta taaactaaat gctttcatac tcttccaagt
     1201 aattgattgc cttaaatttt aatttttgaa ggattctaaa gctagaatag ccccatcaca
     1261 atcagttttg attgattcac aatttagaaa cactatagtt tcactcctgt taaaataaaa
     1321 aggaactgca taaagcaatc cctttctgat tttgaaatca tttacttaac attttatagt
     1381 tgagataatc aatagcttat ctataaaaag agttatagta aaattcctta tttattgatt
     1441 tcaagctccg ctaactgtat ttgaataact gacagttctg caccagcctg aagaagagca
     1501 gctgcattat aggcaccttc tacaattgga accctgttga tgatgatact tttatcactg
     1561 aaatcagtca ccatttttaa gttcatttta gcagaaccta ggtcaaaaaa ggcaagtaaa
     1621 gtatctgctg gattttcgga aacaacccta tctacttgat caaaactcgt tccaattcct
     1681 ccgccctcgg ttcctcctac ataagtaatc ggaacatctt tagctacttt actaatcagt
     1741 tcaacaacac cttctgcaat atgtttggaa tgtgaaacga taacaagacc aataccaata
     1801 ctttccatca aaccactcca gtttctaaaa tagcagtaaa gagtaatcct gatgagaatg
     1861 atccaggatc aatatgtcca agaaaccaca tgctcctaag acaagagcta acagactggc
     1921 catcaataat agtattgttc tttttttcat cattactcct taactagtgt ttaactg
//
LOCUS       DAVLSL010000202         1928 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVLSL010000202 DAVLSL010000000
VERSION     DAVLSL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.204"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..916
                     /locus_tag="VXN30_002016"
                     /pseudo
     CDS             1..916
                     /locus_tag="VXN30_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1033..1836)
                     /locus_tag="VXN30_002017"
     CDS             complement(1033..1836)
                     /locus_tag="VXN30_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1358563.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          670 a          359 c          353 g          546 t
ORIGIN      
        1 gagagtacct gagagcggag aatgccatcc taaaaaagtt aagagaactc cgattgaagg
       61 aggaaaaaga gaaagaagaa agatagaaat tgttcaagaa ttaatgactg agttttcgtt
      121 agatcttctt ctaaaagcca ttaaactagc tcgttggacc tactactatc acttgaaaca
      181 gctagacaaa ccagataagg accaagagct taaagctgaa attcaatcca tttttatcga
      241 acacaaggga aattatactt atcgccgggt tcatttagaa ctaagaaatc gtgcttatct
      301 ggtaaatcat aaaagagttc aaggcttgat gaaagtactc aatttacaag ctaaaatgcg
      361 acagaaacga aaataaagga gacgttggta agaaggcaga gaatctcatt caaggccaat
      421 ttgaaggctc taaaacaatg gaaaagtgct acacagatgt gacagaattt gccattccag
      481 caagtactca aaagctttac ttatcaccag ttttagatgg ctttaatagc gaaattatcg
      541 tctataatct ttcaacttca cccaacttag aacaagtgaa gagtatgtta gaacaggcct
      601 ttacagagaa atactatgag aatacgattc tccatagtga ccaaggctgg caatatcaac
      661 acgattctta tcatcggttc ttagagagta agggaattca agcatccatg tcacgcaagg
      721 gaaacagcca agacaacggt atgatggagt ccttctttgg gattctgaaa tttatggcta
      781 tgagaaaaca tttaaatcac ttaatcaatt ggaacaagct attgtagact atattgatta
      841 ctacaacaat aaacgaatta aggtaaaact aaaaggactt agccctgtgc agtacagaac
      901 taaatccttc ggataaatta attgtctaac ttttgggggg cagtacatat tcaacacctg
      961 atactatgcg ttttattgtg ggaatgtttg cttcattttt tcctaaaatt caatgtttac
     1021 tcagtatttg gattatgaaa aatcgaggtc taaatctaga tacatttttt ctgaagacaa
     1081 atcattttga ccaccgagca agagattttc aaaaaaagct gttaaaaact caggacgtcg
     1141 ctgtaaaatc tttgcattat ctaataccaa ggcatcacga aaatatttgg aatgttgctg
     1201 aaatggtata ttatcaatat caaaaccaaa ctcacgaaga tactgaatca aaaagaccgt
     1261 tactgtccga gtgtttcctt cgcgaaatgg atgaatctgc cagattcctg aaataaaatg
     1321 ctggatttgt ttaaccacat ccgcctgagt tagtgtcgca tatgcaactt gtttttcctg
     1381 attaaaatca taatctaagg tcatttgaat catggagtaa tcagagtaca caacactttc
     1441 accattcaaa acaggttcat tctttgtgat attggtctga cgaaattgcc ccaccggaat
     1501 agagggttca aatatatctt gaaacaactc cttatgaata gcaagtaagg tcgcaggact
     1561 aaagctaaag cctcttcgag acaatagttc tacaatacgt agagaaacca agtctgcctc
     1621 ttccgtactt gcatcaatag tatggtgata agccgttgca tcctcataaa cctgctcata
     1681 agtcagttct ccccgggact gtttctcagc caaagattcc atatacgctg atggcactag
     1741 attgtcaact ttctgcagac caaaacctat ccgccataaa tcacgcttcg cttcataaga
     1801 caagtttgga ttgtcaatgt tgtaagttgg ttgcatagaa atatcctttc aattgttttt
     1861 cttcattacc agtaaatcac gaaattggtt ggaaactggc ttaaactata aaaagtcctc
     1921 acaatagg
//
LOCUS       DAVLSL010000203         1919 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVLSL010000203 DAVLSL010000000
VERSION     DAVLSL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.205"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            140..1843
                     /gene="ilvD"
                     /locus_tag="VXN30_002018"
     CDS             140..1843
                     /gene="ilvD"
                     /locus_tag="VXN30_002018"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1358564.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
BASE COUNT          494 a          411 c          473 g          541 t
ORIGIN      
        1 agtgcactaa tccgctttgt tgtttttaat tttaaatatt tcacatgcgt atagaatttt
       61 ctgaaaattc aagaatttta ttctgttcat tgcctttaaa ttgtgctata atagtaaaaa
      121 ctgaaatggg agggataaga tgactgaatt agataaacgt caccgcagta gcatctatga
      181 cagcatggtt aaatcaccta accgtgctat gcttcgtgcg actggtatga cagataagga
      241 ctttgaaaca tcgattgtgg gagtgatttc gacttgggcg gaaaatacac catgtaacat
      301 tcacttgcat gatttcggga aactggctaa agaaggtgtc aaatctgcag gcgcttggcc
      361 tgtacagttt ggaaccatta ccgtagcgga cgggatcgct atgggaacgc ctggtatgcg
      421 tttctctcta acatctcgtg acatcatcgc ggactccatc gaggcggcta tgagtggtca
      481 caacgtggat gccttcgtcg ctatcggtgg ctgtgacaag aacatgcctg gatctatgat
      541 tgctattgct aatatggata tcccagctat tttcgcctat ggtggaacta ttgcaccggg
      601 aaatcttgat ggtaaagata tcgacttggt ttctgtcttt gaaggtatcg gaaaatggaa
      661 ccacggtgac atgacagctg aggacgtgaa acgtcttgaa tgtaatgcct gccctggccc
      721 tggtggttgt ggtggtatgt atactgctaa taccatggca actgctatcg aagttctagg
      781 gatgagtttg ccagggtcat cctctcaccc agctgaatca gctgataaga aagaagatat
      841 cgaagcagca ggacgtgctg ttgttaagat gttggaactt ggtctcaaac catcagatat
      901 cttgactcgt gaagcctttg aagatgctat cactgtaacg atggctctcg gtggttctac
      961 aaacgccact cttcacttgc tcgccattgc ccatgccgca aatgttgact tgtcacttga
     1021 ggacttcaat acgattcaag aacgtgtgcc tcacttggcc gacttgaaac catctggtca
     1081 gtatgtcttc caagacctct acgaagtcgg tggtgtccct gcggttatga agtacttgtt
     1141 ggcaaatggt ttccttcacg gagatcgcat cacatgtact ggtaagactg tagctgaaaa
     1201 cttggctgac tttgcagact tgactccagg ccaaaaagtt atcatgccac ttgaaaatcc
     1261 aaaacgtgcg gatggtccgc ttatcatctt gaacgggaac cttgctcctg acggtgcagt
     1321 tgccaaggta tcaggtgtta aagtgcgtcg tcacgttggg ccagctaagg tctttgactc
     1381 agaagaagat gcgattcagg ccgttctgac agatgaaatc gttgatggcg atgtagtcgt
     1441 tgttcgtttt gttggaccta aaggtggtcc tggtatgcct gagatgctgt cactttcatc
     1501 aatgattgtt ggtaaaggtc agggagataa ggtggccctc ttgacggacg gacgtttctc
     1561 tggtggtact tatggtctgg ttgttggaca tatcgctcct gaagctcagg atggtggacc
     1621 aattgcctat ctccgtaccg gcgatatcgt tacggttgac caagatacca aagaaatttc
     1681 catggccgta tccgaagaag aacttgaaaa acgcaaggca gaaacaacct tgccaccact
     1741 ttacagccgt ggtgtcctcg gtaaatatgc ccacatcgta tcatctgctt cacgcggagc
     1801 cgtgacagac ttctggaata tggacaagtc aggtaaaaaa taaactcata ctcgaaaatc
     1861 tcttcaaatc acgtcaacgt cgccttgccg taggtatggt tactgacttc gtcagttct
//
LOCUS       DAVLSL010000204         1890 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVLSL010000204 DAVLSL010000000
VERSION     DAVLSL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.206"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..918)
                     /locus_tag="VXN30_002019"
     CDS             complement(<1..918)
                     /locus_tag="VXN30_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1358565.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLSNVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKRTKKMTPKEVAFIEKWINNYPKKCLD"
     gene            complement(1363..1761)
                     /locus_tag="VXN30_002020"
     CDS             complement(1363..1761)
                     /locus_tag="VXN30_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1358566.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          517 a          380 c          324 g          669 t
ORIGIN      
        1 gtccaagcat tttttaggat agttgttaat ccacttttcg atgaatgcga cttctttggg
       61 agtcattttc ttggttctct taggtaacca tctacgaatg agcctgttgt gattctcatt
      121 agttcccctt tcccaagagg catagggatg tgcataataa atgtgctcct cagaaaatac
      181 attagacaag cgattgaatt ccgttccatt atctgccgtg atggaaagaa tcttgtgttg
      241 ttttaagatg agttttagag cctgattgac cacctcagca cttttatttg gaatcaatcg
      301 gatgatctga tgtctacttt ttcgatccgt caagacaagc aagcagtagt ttttcgctct
      361 cgtaagtaga accgtatcaa tctcataatg cccattctcc aagcgaagat tgatagcttc
      421 agaccgctgt tcgatggatt gaccagcagg tttaaagttg gtgctagcct gtttcttaag
      481 cgcttttcct tttctagggt aaagcagatc ctgtttgctt aaccccaatt ttccatgatg
      541 aatccaatag taaatggttg aaattcccac gttaacccct ttagccatca ccatcatttc
      601 aggcgaaaat ttttggttat gatagtggag aatcttttcc tttagttcct tggtcaagct
      661 tgatttcttg accgagcgct tgcgattatt ttcataagac tgttgagcat agtcggcaga
      721 ataaacctct ttgaagcgcc cttttccaag acattgtcgg actgtcccac gcttgatttc
      781 agtgtggata gtttgaggaa cttttccaag cagagaggca atttctctat ttgatttccc
      841 ttcttttttc catctttcga ttaagcgacg gctatcgatt gtcaaatgtt tggcttttgt
      901 agtataattg tcttgcatct ctgtgccttt cttgtgtttg tggttgaaca acaagtataa
      961 cacagaggtg ttttcttatg cctacaagag ctttcattat ttccattttc ttttggattt
     1021 cactctattc tgaaaaactt gtgtatattt actgaagcta gcaagtctta cctgtaaatt
     1081 taatgaaagc aacacaaaat ccgagagggg aatctcggat taatagatag agagttttta
     1141 gtttaaataa attgtttaaa atatcaacaa catcacttct tttcttaacc tgataagtct
     1201 tgattcctaa ttttggggct acgattatat tgtcctcaat atcgtctaga aagacacaat
     1261 ttctaggtta taactggtat ttatcgatag ttactcatat acatcagtcc acctccatac
     1321 ttatgtgcga gcctctcttt gtattatacc tccatactca ccttacagat tcttttggta
     1381 ataatatctt tgcctaatgt agagatagtc ttgcaaagaa aaaacttcct tgtagccatg
     1441 tttctgataa aagtccggtg cctggaactg gtaagtattg acaaaggcaa aacaacaatt
     1501 tcgattctta gcttcacttt ctgcctgttg caatagtttt gaaccgattc cttgccctcg
     1561 cagttcctct tttacaaaca aatactcgat ttctagccaa tttccaaaag tctctgctat
     1621 caaacctgcc aggagattgc ccttttcatc ttcgacataa agattaagtg gctcactttc
     1681 agcctcttct ctttttgaac ggttataaac acgaatcaga ttccctattt cttgcgattt
     1741 atgtgattcc ttattttcca atctaaagta tagtgaaatg aaataaaaca tgcgcaaatc
     1801 gattaaggaa tttaatctaa tttctaacaa tgtcttagaa atcaaagtgt actattttaa
     1861 cttcaatgca ctatacatct aatactcaat
//
LOCUS       DAVLSL010000205         1860 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVLSL010000205 DAVLSL010000000
VERSION     DAVLSL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.207"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(128..1759)
                     /locus_tag="VXN30_002021"
     CDS             complement(128..1759)
                     /locus_tag="VXN30_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1358567.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          568 a          366 c          391 g          535 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatac tatatatctg cagaatactc tttcagtttc
       61 attcagccta gtcttgctta ggaaaaagta aaaagccatc caattatgat ggatagcttc
      121 ttggttctta gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata
      181 cacgagtgta gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc
      241 ctttgttgag gcgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt
      301 catgacgttc tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag
      361 catctttgat aaagtcacta gtttgacggt aaacttcctc taattctttc aaggcggcat
      421 cagaaaattc aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt
      481 ggtctccaat ccgttccaaa tcacgggagg aatcaaggat attggtaagc acttcacttt
      541 ctttttggct gagagcttcg cttgaaaggg caatgagata acgtgttaat tgctcatcga
      601 tggtgttaat tgcttcttcg gttttatgcc ctttttcagc aactttttca tccaagtcaa
      661 tgatgtactt ataggaaagg tcaaaggctt tagcagcgta gtttcctaag tgcaagagct
      721 cttttttagc atttcctaga gcgatagatg gggcttgttt gatgaaatgt tcatcaagat
      781 ataagggttc gtatttgaca acctcgtcct ctccaggaat aatcttggtt acaaagtaag
      841 ccagagctcc gataaatgga aattggacaa tggtgttggt aatattaaag gttccgtgag
      901 caaaggcgat ggtcatttcc ggtgctagat ttagcgtagc ttcaaaccaa tgaatcagga
      961 cagtaaaagg aactagaaaa ataacgcaga cgactgttcc gataacgttg aaggcaacat
     1021 gagctcctgc tacccgttta gctgcaatat tagcccctaa agaggcaatg atggctgtaa
     1081 tggttgtccc gatattgtca ccaaatagaa ctggcaaagc tccctgtagg tcaattagat
     1141 tgccggcgta gaggttttgt aaaatcccaa tggtagccga agaagcttga attagcaagg
     1201 tcaagccggt accgacaaag acacccaaaa caggattctt acttagctca atcatatagt
     1261 ccttaaagac ctgtaaatcc ttgagtggag ccattgcgcc gctcatgaga ttgagggcaa
     1321 aaaagatacc accgacacca aagaggatgc gtccgatatt attgactgtc cgatttttcg
     1381 taaaaaagag acagacggca ccgataaaga gcataggtag ggcatagtta cctaatttaa
     1441 aaccgatgag aaaggatgtg acagttgtcc caatattagc acccatgaca atcccgatag
     1501 cctgacgtaa ggttaagaga ccggcactga ccaggccgac tgtaataact gttacaccag
     1561 aactagactg aattagagca gtcatcccaa taccaaccag aactccaaag aaaggattac
     1621 tagtatattt gtcaatgtaa aaacgaaggc gatctccagc agcttgttgc aaaccgtctc
     1681 ccatggtctt gatgctatat aagaatagcc ccagaccacc taaaaagtga aataaaattt
     1741 cctgccaatt aatggacatt tctttttcct ccgaaaaata atcgcggaat atctcctatt
     1801 ctattttaaa ggataaaagt aaatctaaca agtgttaatg tcaagtttgt aaaagaaaat
//
LOCUS       DAVLSL010000206         1847 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVLSL010000206 DAVLSL010000000
VERSION     DAVLSL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.208"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..1458)
                     /gene="pfbA"
                     /locus_tag="VXN30_002022"
     CDS             complement(<1..1458)
                     /gene="pfbA"
                     /locus_tag="VXN30_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1358568.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRFNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKV"
BASE COUNT          560 a          365 c          248 g          674 t
ORIGIN      
        1 tacttttcct aaatattctg cactatcttt tgcaactcgt atcgcttttg ttttaggatc
       61 ggcaatatta aatatatttt cggcgataac cacctgttta tttaaatcta ataggtcttt
      121 agtgttttta tagctataag catttactaa agctgctccg ctttctcgat aatgtacact
      181 ctctccttta actttcttat caaagcgatt tcctttgatt aatacatcag tgaatcctgt
      241 aaaacgtaca cctgcataca tcatgttatc aaaatgatta tttaaaattt taatattaga
      301 ggggttctgt gtcgacaatg tttgatagtg tgtgccaatt gctgttacta attccccaga
      361 tttatcactt ttgccaaaat aggaattttg aatagtcaca ttttcagatt ttttcccatc
      421 atcattcaag gcataaggaa aaccttttct agttaatggt tcaatctgaa tgctctcctt
      481 acttatgatt tgcccatcct tcatcgtttt gggtaaggct tgcccaagaa aacgagaatt
      541 atcaactaat acatttttcg aacctgcaat ttgaatagca tgcccttgat aactatcctt
      601 gaatgttaca ttttttatag ttacgttatt tgaattccca atagcaaatg cacctgaaga
      661 atttataagt ggtagatttt ttgctttagt tccttcttca ttcaaagcac cgttcatatc
      721 aatcgtacca ccagaataac taatatcttc tgttgggccc cattctactt gcgcaccatc
      781 atccgtaaat aaacctgtca taaaaacaat ggaagggtga ttcttaatat ttataccatt
      841 tagaattgta gctttctcat tcaattctaa gtgtgtatga ctttttaaaa aaacaatttc
      901 ttttactaaa tacgttcctt caggaaaata tacatttcct ccacctagcc cttgagctgc
      961 agcatctatt gcatcttgaa ttgcttgtcg atcatcatga atcccatcac ctaccgcacc
     1021 ataatctttt acacttaaag cagtatcttc aaattctttt ttgataattc tgtcacttat
     1081 aacatcttcc ttataactct tattttcatt agtagatgtt actgtagatt caactgttct
     1141 gttgtctaat tgtgaattga aacgaatagt accattagag ctgatactat taacacttaa
     1201 cgaattatct aaaccattag tggttgaatt agaggcaatc atttcagcag aacgattctg
     1261 attaacagtt ggtgaattat ctaaatcatt agtaattgca ttagaagact cttttgtagc
     1321 catcggtgaa gaactagtaa caacttcatc cgataaaata ccttggcttc ccaaaattga
     1381 aattgccaat agtaccgagc aagtccccac ctttaattta cgaatactga atacctcatt
     1441 aggaacaaaa tatttcatca gaacctccaa tttttttact atttttaaca attgattatt
     1501 acttttctta aaacagatag aatgacaccg ctgtcattat ctttatcata tattttccaa
     1561 attgaattgt caaggaataa cacaagaaaa tattaaaatt tatactcttc ggaaatctct
     1621 tcaaactagg tcagtgccgc cttgccctaa gtctaggtta ctgacttggt cagttctata
     1681 gtagactgaa actataatag tacacctcta cttctaaaac attgttagaa atcgatttaa
     1741 ctgtcccgat taatttgtcc tattcttgtt tcattttact atatctacaa cctcaaagca
     1801 gggttttgag ctatctatgg ccaacttcct agtttgctct ttagcct
//
LOCUS       DAVLSL010000207         1844 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVLSL010000207 DAVLSL010000000
VERSION     DAVLSL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.209"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            22..234
                     /locus_tag="VXN30_002023"
     CDS             22..234
                     /locus_tag="VXN30_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1358569.1"
                     /translation="MANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVRYGIGCHRD
                     FIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            465..1751
                     /locus_tag="VXN30_002024"
     CDS             465..1751
                     /locus_tag="VXN30_002024"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1358570.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
BASE COUNT          522 a          259 c          427 g          636 t
ORIGIN      
        1 ttacaaaaaa tttatgagca gatggctaat ttctatgata gtattgaaga agagtatggt
       61 cctacatttg gtgataattt tgactgggaa catgttcatt ttaaattttt aatttattat
      121 ttagtgagat atggcattgg ttgtcatagg gattttatcg tttaccatta tcgtgttgct
      181 tatcgtttgt atcttgaaaa attggtaatg aatcggggtt ttatttcttg ttgaggtaat
      241 tttagtaaat ttccgaacta atttactctt ttatgtaaag ataatagtaa atagctagta
      301 atttttctaa atcatttttt aatagttgga aatagcaaat ctttctattg tttcttcttg
      361 ataaaaaggc gattttttat tataataaat tgtaagatat aattgcaggt gagagtcctg
      421 ccatgtatgt gagaaaggaa gagcctgagg gctcagacaa aattatgact tcagttgttg
      481 ttgtaggtac ccaatggggt gatgaaggta aagggaagat tacagacttc ctttcagcga
      541 atgcagaagt gattgcacgt taccaaggtg gtgataatgc tggtcacacg attgtgattg
      601 acggtaagaa atttaagttg cacttgattc catctgggat tttcttccct gaaaaaatat
      661 ctgtcattgg gaatggtatg gttgtaaatc ctaaatctct tgtaaaagag ttgagctatc
      721 ttcatgagga aggtgtaaca actgataact tgcgtatttc tgatcgtgcg catgttattt
      781 tgccttatca tatcgagttg gatcgcttgc aagaagaagc taagggcgac aataagattg
      841 gtacgacaat taagggaatt ggtccagctt atatggacaa ggctgctcgt gttggaattc
      901 gtattgcaga tcttttagat aaagatattt tccgtgagcg tttagaacgt aaccttgctg
      961 aaaagaatcg tctttttgaa aaattgtatg acagtaaagc gattgttttc gatgatattt
     1021 ttgaagaata ttacgaatat ggtcaacaaa tcaagaaata cgtgatagat acatctgtta
     1081 tcttgaatga tgcgcttgat aatggcaaac gtgtgctttt tgaaggtgca caaggtgtta
     1141 tgctagatat cgaccaaggt acttatccat ttgttacgtc atcaaaccct gtagctggtg
     1201 gtgtgacaat tggttctggt gtcggtccaa gcaagattga caaggttgta ggtgtatgta
     1261 aagcttatac gagtcgtgta ggagatggtc ctttcccaac tgagttgttt gatgaagtgg
     1321 gagaacgtat ccgtgaagtg ggtcatgagt atggtacaac aactggtcgt ccacgtcgtg
     1381 taggttggtt tgactcagtt gtgatgcgtc atagccgtcg tgtttctggt attactaacc
     1441 tttctttgaa ctctattgat gttttgagcg gtttggatac tgtgaaaatc tgtgtggcct
     1501 atgatcttga cggtcaacgt attgactact atccagctag tcttgagcaa ttgaaacgtt
     1561 gcaagcctat ctatgaagag ttgccaggtt ggtcagaaga tattaccgga gttcgcaatt
     1621 tggaagatct tcctgagaat gcgcgtaact atgttcgtcg tgtgagtgaa ttggttggcg
     1681 ttcgtatttc tactttctca gtaggtcctg gtcgtgaaca aacaaatatt ttagaaagtg
     1741 tttggtccta agagattttt aagatttgtt taagataggt cgggtatact atagacagtt
     1801 acaagaagac ctcctaactt gttgtaacaa atatcctaaa cttt
//
LOCUS       DAVLSL010000208         1816 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVLSL010000208 DAVLSL010000000
VERSION     DAVLSL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.210"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(126..890)
                     /locus_tag="VXN30_002025"
     CDS             complement(126..890)
                     /locus_tag="VXN30_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358571.1"
                     /translation="MFKLSFQSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TSDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(883..1653)
                     /locus_tag="VXN30_002026"
     CDS             complement(883..1653)
                     /locus_tag="VXN30_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358572.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
BASE COUNT          626 a          402 c          294 g          494 t
ORIGIN      
        1 actgaaccgt actatttcaa cttccataaa ttacaatatt gataggcaca gttcccatac
       61 ctgtccaaat tcgaataacg tttagtataa attagaatat gactacaaca tcacaaataa
      121 ttagactatc tataatatct acgatgtaac atatacatag gaagcaccgt tatcaagagc
      181 gccaaggcta tcaaccagat aagatgaaga tacaaacctg tcacctcacc tccccatata
      241 atacgaatca tgcgggtagc gtgatacaag ggagaaaacc aagtcaaatc accaagtatt
      301 gggggcaagg aagtaatggg aaagaaggta tctgaaaaca tataaagagg acggattacc
      361 aaaacattgt agtaattcag aaattcccta gacggtgcca agtaggtaaa gattagtcct
      421 aaaacaccaa acaaataacc cactataaat aaaatcagag gaatcaataa catcaagaca
      481 tgaaaatgcg ctgtgataag catggaaatc agccaaaaaa tcccaccgaa aatcactgca
      541 cgaatacctg cccagatagc ctgacccacc acaatatcac tcgtattgat tggcgtcatc
      601 aacatagcat cataagtctt ttcaatctta tactggataa aggcattgac cgttgtttct
      661 gcggtggccg cagacatggc agtcgcagca attaaacctg taatgacaaa atcacggtag
      721 agcataccgt taacatgcgt caagtaggca ccaactccaa atcccattgc taaaagataa
      781 aagagggggt cgataaggtt ggggacaatt gaaattttaa ataaatttct aaaactcatg
      841 tagttgcgta ccacaacgtg tccgatggac tgaaatgata acttaaacat gcaagtcctc
      901 cttctctgtc ccagtcaacc ccaaaaaaag cgtctcaagg ttgtcagcat catacttgat
      961 gagcaagtct tctacagttc cttcttcttt gatagtccct tggtggagca tcagcaccct
     1021 atctgtcaaa tattcaatct cattcatata gtgggaagta ataatgatag aaacacctaa
     1081 ttccttcaac tccaaaagtt tatcccaaat aatattgcgg gattgaatgt ctaaaccaac
     1141 tgtcggttca tctaaaatga taatatcagg ccgattgatg agagctcttg ccaagacaag
     1201 acgtcgtcgc atcccacccg acagctgatt caccatcttt tgcgcatgtt tgctcagttg
     1261 aacaaaatcc aagagagaat cgattctctt cctaagttcg acagatgaga taccataaca
     1321 tagaccgtga gcaaaaagat tatcataaac attcaaggtc atatccagca tatcctcctg
     1381 ccctacaata cccagacaag atttactctt acgacgattg gtttcaatat tttgccccaa
     1441 gatatcaatc tctcctgagg aaattgaggt cagtgagtaa atcatcttta aagtggatgt
     1501 tttgccagca ccgttgattc ccaagatacc caaacactct ccttctccca attcaaagct
     1561 aattcccttg acagcttcat aagaaccata ttgctttttt agatttttaa cacttaactt
     1621 cttcactctt tctttccccc acaaatagag catgttcgat actttgttaa ttcatctcac
     1681 ttttgaaaaa atgttgagaa tgtaatctta agattaacgt ccttcaactg tggatactgc
     1741 tgcaattact tttgtaattt tacctccatt taaactcaga caataaaatt taaaatattc
     1801 tctagtaaaa catttt
//
LOCUS       DAVLSL010000209         1698 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVLSL010000209 DAVLSL010000000
VERSION     DAVLSL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.211"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..795
                     /locus_tag="VXN30_002027"
     CDS             <1..795
                     /locus_tag="VXN30_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1358573.1"
                     /translation="LTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRHEGF
                     IPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKIIDT
                     RNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKDWIR
                     TAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKTIDL
                     PFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            797..1606
                     /locus_tag="VXN30_002028"
     CDS             797..1606
                     /locus_tag="VXN30_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1358574.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT          580 a          304 c          296 g          518 t
ORIGIN      
        1 ctaaccgttg aagatgccaa acaaattgaa ttagaaattt tggattatat tgatactctc
       61 tgtaaaaagc acaatatcaa ctatattatt aactacggta ctctgattgg ggcggttcga
      121 catgagggct ttatcccttg ggacgacgat attgatttat ccatgcctag agaagactac
      181 caacgattta ttaacatttt tcaaaaggaa aaaagcaagt atgaactcct atccttagaa
      241 actgataaga actactttaa caactttatc aagataaccg acagtacgac taaaattatt
      301 gatactcgaa atacaaaaac ctatgagtct ggtatcttta tcgatatttt ccctatggat
      361 cgctttgatg atcctaaggt cattgatact tgttataaac tggaaagctt caaactgctg
      421 tctttcagta aacataaaaa tattgtctat aaggatagcc ttttaaaaga ttggatacga
      481 acagcctttt ggttgctcct tcgaccagtt tctcctcgtt attttgcaaa taaaatcgag
      541 aaagaaattc aaaaatatag tcgtgaaaat gggcagtata tggcttttat cccttcaaaa
      601 tttaaggaaa aggaagtctt cccaagtggt acctttgata aaacaatcga tttacccttt
      661 gagaatttaa gccttcctgc acctgaaaaa tttgatacta ttttgacaca attttatgga
      721 gattatatga ccctaccacc agaagaaaaa cgcttctaca gtcatgaatt tcacgcttat
      781 aaattggagg attaggatgc aatatttaga aaaaaaagaa attaaagaaa ttcaactagc
      841 cctgctggac tatattgatg agacttgtaa gaaacatgat attccttatt ttctcagtta
      901 tggaaccatg cttggagcca tccgccacaa aggtatgatt ccttgggatg atgatattga
      961 tatttccctt tatcgtgagg attatgagcg tttactgaag attattgaag aagaaaatca
     1021 ccctcgctac aaggttcttt cctacgatac atcttcttgg tacttccata atttcgcatc
     1081 gattttggac acttctactg ttatagaaga ccatgttaag tacaagcgtc atgataccag
     1141 ccttttcatc gatgtcttcc caattgatcg atttacagac ttgagcattg tcgacaagag
     1201 ctataagtat gtggctcttc gtcaactagc ttatatcaaa aaatcacgag cagttcacgg
     1261 tgatagcaaa ctaaaagatt ttcttagatt atgtagctgg tacgctctcc gatttgtcaa
     1321 tcctcgctac ttttacaaga aaattgatca actagtcaaa aatgctgtaa ccaacattcc
     1381 tcaatatgaa ggaggagttg ggatcggtaa ggaagggatg aaagaaatct tcccagttga
     1441 tacctttaaa gaactgattt taactgagtt tgagggccgt atgttgcctg ttcccaaaaa
     1501 atatgaccaa tttttaaccc agatgtatgg cgattatatg acaccaccat caaaagaaat
     1561 gcaagagtgg tatagtcata gcattaaagc ttatcgcaaa aactgattga gggggattat
     1621 acaaactact aagatagagg ttattcaaaa acataatttt agtagaaaat gaaatacata
     1681 ttcccacaat aaaacgca
//
LOCUS       DAVLSL010000210         1688 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVLSL010000210 DAVLSL010000000
VERSION     DAVLSL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.212"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..604
                     /locus_tag="VXN30_002029"
     CDS             <1..604
                     /locus_tag="VXN30_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1358575.1"
                     /translation="EHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIE
                     SGSTCALLAEEICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPL
                     LKEMIQTFHVRQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDK
                     RGTVRRFALSQVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            617..1597
                     /locus_tag="VXN30_002030"
     CDS             617..1597
                     /locus_tag="VXN30_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1358576.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          609 a          187 c          342 g          550 t
ORIGIN      
        1 agagcatgga tatgctgtat taaatagtgg agatgactta aatgtacgtt tgtcaattaa
       61 ctatgaaatt aagagaaaaa ttgttcagga agcagtaaaa ttggtgtcag ataatgaaac
      121 aataatgata gaatctggat cgacctgtgc tttacttgct gaggaaattt gcaagcaaaa
      181 aagaaatgtt acgattgtaa caaattcatt ttttatagca aattttgtga gagcttatga
      241 ttcatgtcgt attattgttc ttggtggaga gtttcagaag gattcacagg ttactgtggg
      301 acctttatta aaagaaatga tacagacttt tcatgtgcgt caagcttttg ttgggacaga
      361 tggctacgat aaagagatgg gctttacagg aaaagattta atgcgcagtg aggtagttca
      421 atatatttca gcagcgtcgg ataaagtcat tgtactaact gactcaagta aatttgataa
      481 aaggggtaca gtaagaagat ttgctttaag tcaagtctat gaagtaataa cagacgaaaa
      541 actttctaaa caaaatatag ctacattaga aaatgctggg ataatggtta aggtagtttc
      601 gtaagaggtt aagtgtatga atcaagatag gaataaactg ctctctaaaa ttgcttatct
      661 gtattatatt gaaaacttaa atcagtcaca aatagcagca aaattaggaa tttatagaac
      721 ctctattagt agaatgttaa cagaagcaag gaatgcagga attgttaaaa ttgaaataga
      781 gaattttgat accaatatgt ttaagttgga aaattatgta aaagaaaaat atggtctgga
      841 aagtttagaa attattccaa atgaatttga tgataatcca acaattttat ctgaaagaat
      901 ttctcaagtt gcagcaggtg tccttaggaa tctaattgat gataatatga aaattggctt
      961 ttcttggggg aaaagtttaa gtaatttagt agatttaatt cacagtaaaa gtgtccgaaa
     1021 tgttcacttc tatcctctag caggtggtcc tagtcacata cacgctaaat accatgtgaa
     1081 tacactgatt tatgaaatgt ctagaaaatt tcatggagag tgtacattta tgaatgcaac
     1141 gattgtgcaa gaaaataaat tgttagcaga tggtattttg caatcaagat attttgaaaa
     1201 tttgaaaaat agttggaaag atttagatat agctgtagtc ggaattggtg attttagcaa
     1261 taaaggaaag catcaatggt tagacatgct tacagaggat gactttaaag aattgaccaa
     1321 agttaaaact gtaggagaaa tttgttgtcg attttttgat tcaaaaggta aagaagtcta
     1381 tgaaaactta caggaaagaa cgatagcaat ctctttggaa gatttaaaaa atattcctca
     1441 aagtttagct gttgcttacg gtgatacgaa agtatcttcg attctttctg tcttgcgtgc
     1501 taatttagta aatcatttga ttacagacaa aaatacaatt ttaaaagttt tggaagaaga
     1561 tggggatttg acttttagag agattctagg tgagtgaaaa tgatagactg attcagttta
     1621 tcgtttttct ttttagttga ttgcacattt gtgcttatat aaacaaaaat agtttatctg
     1681 ttgttttt
//
LOCUS       DAVLSL010000211         1683 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVLSL010000211 DAVLSL010000000
VERSION     DAVLSL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.213"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            128..655
                     /locus_tag="VXN30_002031"
     CDS             128..655
                     /locus_tag="VXN30_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1358577.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPIVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            667..1182
                     /locus_tag="VXN30_002032"
     CDS             667..1182
                     /locus_tag="VXN30_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1358578.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1179..1646)
                     /locus_tag="VXN30_002033"
     CDS             complement(1179..1646)
                     /locus_tag="VXN30_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1358579.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVIYYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
BASE COUNT          472 a          363 c          314 g          534 t
ORIGIN      
        1 actattctag tttcaatcta ctataacttt taactaaaaa atgatctata aacccaagta
       61 aattcttgct ttattgtctg gtttattgta ccatactagt gtatatgcag ttaaaaagga
      121 gattcttgtg aatacacgga aaaagacaca atttatgaca atgacagccc ttttaacggc
      181 tattgcgatt ttgattccaa ttgttatgcc tttcaagatt gtcattccac ctgcttccta
      241 tactttgggg agccacatcg ctatttttat agccatgttc ttgtcgccct tgatggcagt
      301 ttttgtcatc ctagcctcta gttttggatt tttgatggct ggctatccca ttgttatcgt
      361 ttttcgggcc ttttcccata tatcttttgg tgctttagga gctctttacc tacaaaaatt
      421 ccccgatacc ctagataaac caaaatcttc ctgggttttc aattttgttt tggctgttgt
      481 tcatgccctt gctgaagtat tagcctgtgt ccttttttac gcaacttcgg gtaccaatgt
      541 agaaaacatg ttctatgttc tatttgtact agttggattt ggcacaatta tccatagtat
      601 ggtagactat acattagcac tagctgtcta taaagtgctt cgaaaacgcc gttaaaagga
      661 aaagctatga caaaagatcg caaacaagcc ctgctccaac tcttgaaaga agctcctaaa
      721 gcccttaatg gccaaagttt ggcggaacat tttcatgtca cacgtcaggt cattgtgcag
      781 gacattgcaa ttttgagagc cgatggcgct cctatcctat ccaccaatcg tggctacatc
      841 tataagcaaa ttgataccaa tccttatgtt cacaaacttt tcaaagtgaa acatgaagtt
      901 gaagaaatcg gtcaggaact ccttgctatt gtcgataatg gtgggcatgt ccagaatacc
      961 ttgattgacc atcccgttta tggagaaatt gaaaccttgc ttaaactgtc ttgtcgcaga
     1021 gatgtgcaac attttctaga acaagtcgag cattctgact ttagacctct atctgaattg
     1081 acagatggca tccattacca cctagtcgaa gccgaaacac aacaagacct ccactatatc
     1141 gaggaggcct tggatcagct aggttattta gtaaaagact agaagatttt cttttcccaa
     1201 tcgtcttctg tacggcgagg atagaaaaac tctgacttgt cgggagcttc catcatttcc
     1261 atcaatggta gcatatcata ggccagatcc aagtttggaa tctggtcttt ttgcacccaa
     1321 gaaacttctc cttcatctga agaccgaagg ctaccagaga actcagtcgc cttataataa
     1381 atgacaatgt agcgcccacc tgtatctagt ggccaatttt taatgccgac aagttgagga
     1441 ttttggatag tcaaccctgt ttcttcatag atttcacgaa tgacagactc tgcgaaagac
     1501 tcgccatttt ctacatgacc tccaggaaag gcataaccag accagcgatt gttttcaggg
     1561 gcgcgatact gcatcaccac gcgctgagtt tcgaggtctt caatcagaca gatatttgtt
     1621 aaaattgtta attgggaacg ggacataaat ttactcgctt tctaatttat taaattttca
     1681 gac
//
LOCUS       DAVLSL010000212         1674 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVLSL010000212 DAVLSL010000000
VERSION     DAVLSL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.214"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>1674
                     /locus_tag="VXN30_002034"
     CDS             <1..>1674
                     /locus_tag="VXN30_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1358580.1"
                     /translation="KLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKSNLS
                     NNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTLGKM
                     DVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRTRQI
                     KEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFFSLL
                     LTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVSGVF
                     LQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQIVAA
                     LKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGANLQ
                     NKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQIIPDG
                     EKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILYKSL
                     FSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKII"
BASE COUNT          590 a          231 c          292 g          561 t
ORIGIN      
        1 aaattatgta tcattggatt cttcttttgt ttgattgcta caattggtct ggtcactatt
       61 agtgataccg aaatccccat acctttacca attgatggag ctttttctat ccaaggaaaa
      121 agtaatcttt ctaataacga aatatacgaa atggttcgtg acttatcaaa aacggaaaag
      181 gttaccattt acaagccaat cgttcagagt tcgggtcagt tgaagtatgt aaattttgat
      241 gatgtaaata atgaacaatt aaagtcagca ccaatagtag ggatgtatta cacacttggg
      301 aaaatggatg tagacagttt gaaaccactt acgatgacgg gactacaaac agtctatatg
      361 gcttatcctt ggtatatagg aggaatatta caattcactg gaactttaag aatactgtta
      421 atgggttcaa tttatttaac tttattagtt gttttatttg ttgttaggac gaggcagatc
      481 aaagaaggag tgatacgacg ttctttgggc ttgcctatat acgacttaag aagagagtat
      541 ggcatttctc ttatttttga actgattatg atggctttat tgatgatttc ttacagttct
      601 tttttgggta atggtttctt cacctatagt tctaagttat ttttctctct gcttttaacg
      661 aattttatac tatttcaaat cattgatctt attacttttg ttttattttg gttgacgatt
      721 cagattgaaa agcctattga aattattaaa aacaaggcaa aaaacaagct tatttttgtc
      781 gtatggcttg ctattatttc gattatcatc cttgtctcag gagttttttt acaagaaaca
      841 aagagtagtc aatctagtat taacatacag atacagaatt tagtaccatg ggatacagta
      901 aaagactgga gaaggattga gttccttgga attgaaagta actctactaa aaatagagaa
      961 gttaatgatt cagatggtca gtatctacag atagttgctg ccttaaaaaa cttagatttc
     1021 ttatatatag aacggtcttc agcatatgtt ccagatttca tgaaaacttc acatgttata
     1081 gaaaattttt ctaaacaatt agaaaatgac gggataacga atccagaaat aaataaagag
     1141 ttaatttata tcaaccaaac aggtgctaac ctacaaaata aagtgaatgg aacaaactat
     1201 catcttttag ataataagat agcaaccatc tatattcctg agaagtggaa agaaaaccag
     1261 aaatctattg agaatacagt tgtagcagaa caatttattg gaacaaacta tacaagagaa
     1321 cagcttgcag ttcaaataat tcctgatggg gaaaaaattt tttattttaa tgaggatgct
     1381 gataataatc ttaaaatgaa agatatttta ccgctggcaa atgtagcaga tagcaaagat
     1441 aatatagttg ttgtgttaga tacggacaaa atgatggaaa ataataagtt ttctttggct
     1501 agtaacatac tatataaatc tctttttagt cctgaagcgg taaaaaaaat aaatgagatg
     1561 actgtcctgt tgaatttctc aatgaatcca gtagatgttt accaaattgt gaaattaaag
     1621 attcaatcct tagagcacca aattttactt tcacaaatct tgcagaagat aatt
//
LOCUS       DAVLSL010000213         1668 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVLSL010000213 DAVLSL010000000
VERSION     DAVLSL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.215"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..>1668)
                     /locus_tag="VXN30_002035"
     CDS             complement(<1..>1668)
                     /locus_tag="VXN30_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1358581.1"
                     /translation="EFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYR
                     TSLRPGIQAIDSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDH
                     FDTARVMVGINPVEFRFLLNPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPN
                     FSQASRPIILNSWETFYFDLGTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLG
                     DWVTDRSRLPEGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREK
                     SVGRNQYVLDLTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQM
                     EFGHRYILGLYQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIER
                     LKIQHGTSYGYSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVE
                     EKEQVREQIQFYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSK
                     LNPGYTRVYFSGLDKDKCYSVSGFDEFFYGDELMNAGIKVSLSNLAL"
BASE COUNT          569 a          342 c          223 g          534 t
ORIGIN      
        1 caaagtgcta aattacttaa acttactttt attccagcat tcattaattc atctccataa
       61 aagaactcat caaatccaga gacggaatag catttatctt tatctaaacc actaaaataa
      121 actcttgtat aacctggatt caacttacta ttcaattgag catacaataa aatcgattgg
      181 catttatctt ttgaaacaac ttgccaacta gcagaatcac aactaaaagg actgtttatc
      241 ctatagaaat ccccatattg aaacaatgaa cgatattttt tataaaactg aatttgttct
      301 ctcacttgtt ctttttcttc taccgacaat ctcgtaacat ctaattcata accgaaagaa
      361 ctaaaataag ctacatttgt cctagtgtcc aaactcgttt gtcttccact ttgttcattt
      421 ggagaaatag aaacatgggc tgtcatcatt gatggagaat atccataaga agttccatgc
      481 tgaattttca atctttctat cgggtccgta tcatcactcg tccacgcttg cggtgcataa
      541 tacataagtc ctaaatcaaa acgtccacca cctgaagaac aagattcaaa tagaactgaa
      601 gggaacttag ttattaaacg atctaataac tgataaagac ctaagatata tcgatgacca
      661 aattccatct gctgttcaga atcaagttca atactataaa tatctgttat atgacgattc
      721 atatcccatt tgatataatc cagatttgtt ttgattatga ttttagaaat agaatcaaaa
      781 aggtaatcaa caacttcctg tctcgtcaaa tccaacacat attgatttct tcctactgac
      841 ttctctctgt ctaacaaatg gatagtccaa tcggcatgat tcttgtacaa atcactatca
      901 atagaaatca tttcaggctc aaaccacaag ccaaattgta aacctatttt gtgaatttca
      961 tctgcaagaa atccaatacc ttcaggaagg cgacttctat ctgttaccca atcccccaga
     1021 gaacttttgt catctttcct atgaccaaac caaccatcat ccagtacaaa taattctatc
     1081 cctaaatctt tagcagcctt tgctaaatct aaaatttttt ctgtacccaa gtcaaaataa
     1141 aatgtttccc aactattgag tattatagga cgactagctt gagaaaaatt aggattaact
     1201 aaatgatgtc gataaaaatc tgatagttgt tggctcatct gattcatccc ttgatcagaa
     1261 tagatcacaa ttgcttctgg tgtcacaaaa ctttcggcag gatttaataa aaaacgaaat
     1321 tctactggat ttattcctac cattactcta gcggtgtcaa aatggtcaac ttcaatcata
     1381 ttttgaaaat ttccagaata gataaagtta aaaccataaa cctcaccact atgctctgta
     1441 gtttccctcc ttgatagcat aaagaaagga ttttgctgag gacttgatga gtatctcaag
     1501 ctatctattg cttgaatacc tggtctaagc gaagttctat ataactgacg ttccctcagc
     1561 catgctccag aaaattgaat aaagtcatat tgactatcag gtagatctag ctgtaagctc
     1621 attgctttca aaagagtcaa cttattattg ctattattta aaaattcc
//
LOCUS       DAVLSL010000214         1639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVLSL010000214 DAVLSL010000000
VERSION     DAVLSL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.216"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(343..768)
                     /locus_tag="VXN30_002036"
     CDS             complement(343..768)
                     /locus_tag="VXN30_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358582.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(896..1279)
                     /locus_tag="VXN30_002037"
     CDS             complement(896..1279)
                     /locus_tag="VXN30_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358583.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKETVLG"
     gene            complement(1301..1507)
                     /locus_tag="VXN30_002038"
     CDS             complement(1301..1507)
                     /locus_tag="VXN30_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358584.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISRVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(1494..>1639)
                     /locus_tag="VXN30_002039"
     CDS             complement(1494..>1639)
                     /locus_tag="VXN30_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358585.1"
                     /translation="CDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKGERNITN
                     GFK"
BASE COUNT          500 a          354 c          190 g          595 t
ORIGIN      
        1 tgataaaata accaaaaact actcgctatc ccctctcaca ttaccctaca tatcgtttga
       61 catgcgactg atagttcagg aaaacttcaa ggagaacttt tctctcatcc actatgcagg
      121 acttactatt tcacttctac tcctataggc tcaatttgac acttttcttt ttcgaattgc
      181 tcatatgcct cctgaatagt gagttcgatt tatccaaatt tatttttgtc tattttggtg
      241 aatacttcca cgaagatagt ctgaaaatga atttgcacca attttccttc tctttttaag
      301 catattattc cagatatgat tgccctcttg ttcagtaata aattacgctt taaacgcttc
      361 aatcagtata tctcctgttg tcatatgttc taaagaaaat tcttctacat atggtttaac
      421 atctcttagg ttattacttc ctaatatccc attatgcttt ttcgctaaga aaatagaagt
      481 cgcttctccc ttaccaataa tcttgttact atcataattt cttgttaaat ctctatataa
      541 tgcgtattct tcagttccaa tgtctatgct cacaatctca gctgaaccct tagttaccaa
      601 ctggtctatc ctagatttta aatggggaat tgtaggtata ttgatttcat catacacctc
      661 ttgtggaata acaattttac ccgaatagag cttttctaaa agatgttcag tgccaaccca
      721 taaaaatgct gaaatacaat ctgcatcaat aaatacacga ctagtcaaac gacaactccc
      781 cctcttcatc tagcccatat acaatatcat atctgaaagc atctagtaac agttcctcat
      841 acttcccttg cgaaattctg ttattttcta aaagttgttc agttaaatta atatattatc
      901 ctaataccgt ttccttttta ctttctgaca aaggacgata taaacttgta tcatagccta
      961 atcttgaagc tgtctctata acactaatat ccatattttt aatttcttct gcatcaaggt
     1021 atccatcatt cctcaatcta tataacatag ctttatgact gataccataa aactgaccca
     1081 attttataat atcttctact tcaagatgag ttctattggc attttctcta atttcctcaa
     1141 ccatcctata cagtgaagat gggaaaatta aaaaataaga agcaaactga tccgcttttc
     1201 tttcagtttc atctccttca ccaatcaaga taagactgac tgaactcttc ttcacctcat
     1261 cataataaag atgatacagt tcatgtgcta aagaaaatct ttaccttcct aatgacatgt
     1321 ctgaattgac tgcaatgaga ctgaactgag ttcctttata acagactctg ctaatattct
     1381 ttccgagtcc ataaaatacc agcgtcaaat tttctatctt ttgtaccaat ttaaaaatat
     1441 ctatcggcga ttcaccatca gctcccaact tttttctaag atttggagct ttattactta
     1501 aatccattcg tgatattcct ttcacctttt tccattttgg caatcatgct ttgattcaaa
     1561 gacaaatact cagcaatctt atcttgagta agatttgaag atattctaag ttcttttatt
     1621 ctttttccaa catcacata
//
LOCUS       DAVLSL010000215         1631 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVLSL010000215 DAVLSL010000000
VERSION     DAVLSL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.217"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..959)
                     /gene="ltrA"
                     /locus_tag="VXN30_002040"
     CDS             complement(<1..959)
                     /gene="ltrA"
                     /locus_tag="VXN30_002040"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEV1358586.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAVSKCVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLS"
BASE COUNT          419 a          384 c          282 g          546 t
ORIGIN      
        1 gacaaattca gttgctcaat acgtcttgtt aggtctatac tccatttcct ctgtgttagt
       61 ttcttcaatt taaacttaaa tctccgaaca ctatcttgat gtggacgact tttccaacca
      121 tctgatgatt tccagaaccc aaaacctaga tatttcaact ctcttggtct ggtaatctta
      181 gtcttggtca tgtttacttt caaacctagc cgtttctcaa taaaacgact gactgaatac
      241 atcacacact tagagactgc ctcgcttccg accgtaatca cacaatcatc tgcgtagcgc
      301 acaaatcgaa gtcccctctt ttctaattcc ttgtccaatt cattaagcat gatattggat
      361 aagagaggag ataaatttcc tccctgtggt gtaccaacta gtgttttata acgttgacca
      421 ttaatgataa cacctgaatg aagatactta cgaatcaagg attccgtatc tccgtcttcg
      481 ataatgttat gtactaagga catcaatcta tcttgaggaa ctgtatcgaa aaatttctct
      541 aggtctatat ccactatcca ctcatagccg tcatttaagt attctaagag cttcatgatg
      601 gctttttcac atgacctatt tggtctgaaa ccataactcg tatctgagaa atggggttca
      661 caaatggggc tcatgacttg gacaatggcc tgttgaatca ttctatccat aactgttgga
      721 attcctagtt gacggatgcc tccgtctggt ttaggtatct caactctaag aactggttga
      781 ggcttatatt ttctctgttt tatcagttcc ttagtcaggc gccagttttg tctgagataa
      841 ttatccatct cttcgatagt cattccatca atcccagctg agcctttatt ggattttact
      901 tgattgtagg cttccagcat attttcgcgt gataatatct tatctagcag ttttgacatg
      961 cgcgtattcc tttcttgttt tggtgcctga gggacatctt atattggtga accttcatct
     1021 agtcattacg tgtcaaagtc cagttctatc agaccactca ttttacagac acaagtgaag
     1081 taggattact tcgatctgtc gttttctctt tctagcgaca gacctcaaac agtctctccc
     1141 cagactgttt gagtattcac cccttcgctc cacttccatt acggaagttt cctcactact
     1201 atgggctcgg cttacttctc atgattcgtt gttactacgc tttcagcgct catgaaacct
     1261 cacgggataa gtcgtacatc tttcctcgtt tatacaagat atgaggacgt taaaaggtta
     1321 cagtgaaaat aggaaatctg acgcagagtc tttagactct agaaagattt atctttttca
     1381 cacaaacctt agtccgaatt caattactct cgtgatttac acatataggt tacgactacc
     1441 tttttggact ttagagtttg aagcctcttt atccgctata tacgccttac tatcacgttc
     1501 ctgttcgtag agccacgatt tcgctatccc ttcctctccc tgctacctca cgatagtcaa
     1561 gcttgggaat cgcctattgg gttcaccggt agcaggtgcc caccgaggac ttacacctca
     1621 gatatacgac a
//
LOCUS       DAVLSL010000216         1589 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVLSL010000216 DAVLSL010000000
VERSION     DAVLSL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.218"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(42..583)
                     /locus_tag="VXN30_002041"
                     /pseudo
     CDS             complement(42..583)
                     /locus_tag="VXN30_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(659..1435)
                     /locus_tag="VXN30_002042"
                     /pseudo
     CDS             complement(659..1435)
                     /locus_tag="VXN30_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1359..1553)
                     /locus_tag="VXN30_002043"
     CDS             complement(1359..1553)
                     /locus_tag="VXN30_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1358587.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
BASE COUNT          425 a          354 c          304 g          506 t
ORIGIN      
        1 ttttagcagt cgatgcgtac tgttatagat tcaacgcact ataattgatg acgtttagct
       61 gtttccaaca aactcataat aatagctctt gctttagctc cttcaaaact ttgagaaaat
      121 aaccaccctc gcttttttaa actgagctca ggctaaaaga gtccactgga ctcttttact
      181 ccgtcccata accaatgatt taatggcgcg ttcagctaga ttattggaaa ggaccagatg
      241 tccgtctttt aaaatggtct taaaggtttc tttatacttg aggctgtatt caattgccct
      301 tcctagtttt gaacctgcta aaactgactg acggcggcac caagcaaaga agtcttccat
      361 taggggctgg acgtttctgt agtcgttcat cagctggcaa agcctcccag tctctttcca
      421 aggcaaataa ctggtcacga taggctaaac ctttagctcc taaggatgat ttatctgctt
      481 gcttgggggt cgcttcaaaa aatttccttc tcacatgcgc ccaacatcca acaagttcag
      541 cttcttccag ttgacgataa gcttctagcg gttcgcagtt taccaagctt agcgtcgcct
      601 tgctcctaaa ccttagactg ctatcgcata ggcagaacta gaggactaaa gttctaagtt
      661 actgccgcaa catatcacaa tgcatatagc cagaataatt tcctaggaat tcttgcacta
      721 ccgaaccact ccgacgctga tcatggtggt acagcgtaat cccttgtttc tcagctttcc
      781 cagacaaaaa agtccaatag taagtcagct gactatcact ttctaagacc cgataagagg
      841 tttcatctgc atgaagaaga gcttgttcta acaacttttc tcgtaaaagg ttataaaggg
      901 actctaaata gtattgactc gccttgatat gccaattagc aatttcctta cgtgtgattg
      961 gtaaacccat cttagcccaa tcttcttctt agcgataatt gggtaccttc agaataaact
     1021 tctgatggat ggtgtgagcg ataatagaag ctgagccaag gctatgcgcc aaaggggctt
     1081 taggaatagg agctttcacg attttatcac ttggattatt cttactgcat gtttggcatt
     1141 tgtaagcgtg ttggatatga ttcactcttt ttaattgcgc aagaataaag accaattctt
     1201 gtcgttgaag gctcgctcca atctctttta gctctccctg acagtcaggg caaatgcagt
     1261 tctctaagcg atgatgaact tcttctgaat caaattgggc aagaatagct tgacgtttcc
     1321 ctttagcttt cttacgttta taggtgattt cttctctttc aactgggtaa gtcagagtct
     1381 tcttccatat tttgttcctc ttcaaaaaga ctgagttgtc cagatgggca aacacttttc
     1441 tcagaggatt ttccatagag cttttgcgtt agataagcca cttgttcacg aagaagggca
     1501 agttcattgg tgagactatc aattgtagca ctctgttgtt gaatgatttt caatcttctc
     1561 ccatagactt tcctccttat ttcctaact
//
LOCUS       DAVLSL010000217         1538 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVLSL010000217 DAVLSL010000000
VERSION     DAVLSL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.219"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(927..1499)
                     /locus_tag="VXN30_002044"
     CDS             complement(927..1499)
                     /locus_tag="VXN30_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358588.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT          466 a          255 c          256 g          561 t
ORIGIN      
        1 caaatttata ctaatacatt ttgtaaaaaa gcgagtttcg attgaaattc gcttttttaa
       61 atgctgtgga tttgggtcaa tcttgtctaa aaatagggaa atcctagata cagtgaaggc
      121 tttaaatgct ggtttttact gtcttcagcc ttatattttt tcgtagttgg ttacctcaca
      181 tctcttgtat tcgcttacat aaagtattat aatataattg taggaaagaa ggtgttttta
      241 tgatatacac acttaaattg gtgttgttta ttacctttct tgtaataagc ttgttacctg
      301 ataagatttt tggaaaaaat aaaaaaattt ggaaaatagt ttttgcaata ttgacggcag
      361 tggcagcatt gtcatttatg tactaagtta ttttaagaat gtagggaaat aaaccctaca
      421 ttctttttag ttttttctgt tttctaaatt ctatttatcc aagcgattca acatttcttg
      481 cttcttcgct tcaagttctg cacgcttttc ttcgatttcg gcatgttttt tctcgagttc
      541 agaacaactt gcaccattgc taaattcttt tcgccatcag gagatagggt gagtcgacat
      601 gtctattact cacccaaagc agtcctacaa agcaggaatt ttctgttact tttttggaaa
      661 tagtaacgtt tatacagctt tgacacttcg tatcaaagcg ccaaacacac tccgagggat
      721 ttacagaaag cagaaaagga atgatctggt ataagatcat tccttttctc tctttttctt
      781 taagtaatta tatacaatgt acgacgaagt cgtcattgca atgctgatcc accacctaaa
      841 gggaacttta aacaacattg ataagataaa gaatataaac aacgaaaata cgttataccc
      901 aattaatttt attgtatatc tcatgattaa aagttaatcc ttccgttgtt aggaatggca
      961 tcatttttat cccataattg tgctaaataa gtccccggtg ataataaatt catagcgaat
     1021 tctaaagcaa catcatttac aaaccaacta cctagatatc tagaaattgc tgaacgaata
     1081 gcactttttg ctgcatgttt tccttttact ttaattagat ttgcaaggcc tgcagtagtt
     1141 cctcctaatg ctaaagctat tgcagtatct aatagagcac ccatttgatt aactgtaata
     1201 ccttgccaag ctgctctaaa tggagagtat gtaggtggga ttgtataatc gccttgtaat
     1261 tgtcggttaa ttacttcttt gatccattgt tgtgagacgt ctggatgaaa agattggatt
     1321 tcgtttgcaa gtgtattgat ttgttcttct gttagagaag tgacaggttg aagttccata
     1381 tttgtttcaa tttgtgatac ttgttcagaa gcgtatacag ctgaaacact tggaatcgct
     1441 gatacaatta acacaattga cgtcaaaaaa accgaaataa atttcattaa tttgttcatg
     1501 agcttttctc ctttttattt gtatctgctt acatttta
//
LOCUS       DAVLSL010000218         1537 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVLSL010000218 DAVLSL010000000
VERSION     DAVLSL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.220"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            17..856
                     /locus_tag="VXN30_002045"
                     /pseudo
     CDS             17..856
                     /locus_tag="VXN30_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809258.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            872..1351
                     /locus_tag="VXN30_002046"
     CDS             872..1351
                     /locus_tag="VXN30_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358589.1"
                     /translation="MKLNKQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMM
                     MNTSNDLFIKAIELLPFYSLPVFVASNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
BASE COUNT          539 a          190 c          249 g          559 t
ORIGIN      
        1 taatattaat ggaggaataa aaaatgtaag taagcattat ggtcattcaa tcattctcaa
       61 agatataaat tttgcactta acaagggtga aattgttggt ctagcaggga gaaatggagt
      121 tggtaagagt acgttgatga aaattcttgt tcagaataat caaccgactt caggtaatat
      181 tataagcagt gataatgttg ggtatttaat cgaagaacca aaattatttt tatctaaaac
      241 aggtttagag aatttaaaat atttgtcaaa tttatatggt gttgactaca atcaagaaag
      301 atttagatgt ttgatccaag agttagattt gactcagtct attaataaaa aagtaaagac
      361 ctattctttg ggtacaaaac aaaaattagc tttgcttcta actctcgtta cggaacctga
      421 tatattgatt ttagatgaac cgactaatgg tttagatatt gaatcatcac aaatagtttt
      481 agcggttcta aaaaaattag ctttacatga aaatgtggga attttaatat cgagtcataa
      541 attagaagac attgaagaaa tttgtgagag agttcttttc ttggagaacg ggcttttgac
      601 atttcaaaaa gtaggaaaag atagtcataa tttcttgttt gagatagctt tttcatcagc
      661 tacagataga gacattttca ttaccaaaca agaattttgg gatattgttt aggaagaggg
      721 attgagaatt actatgtctg ggaatattca aaatagtgag ctttttaaat tttttaacga
      781 aaactctatt aaagtagttg attttgaaac taaaaaagag acgcttaaag atatttacct
      841 aaatcgttca aaataaagga aggttataat catgaaatta aataaacaga agaatcggat
      901 gatttacgtc ttgtctaatt ttctatatgc catctcagtt tccattattt atgctttgaa
      961 tggcattgtg ttactagtca tagtaagtaa attgggtatt ccaggtgatt taggattaga
     1021 ttttatagta gctattgtag tcaatacaat tttgttagtc ctgttttatt ttctattatc
     1081 ttacattttc tatttataca aattgaaaag tggcttggta tttggtattt tagtagcttt
     1141 actactcttt atccctaata tattaaatac gatgatgatg aatactagta atgatttgtt
     1201 tatcaaagca attgaacttc ttccttttta ttctttacct gtatttgtgg cttcaaatac
     1261 gatgtctatt agtcagtatc ttgtggtaat cactacaatc attttattgt actttttcac
     1321 tctcaagaaa agcaagaagt attcatttta gtttcattta gtattatttt gcatacctaa
     1381 aatacaataa aaaaatcagt catcttgcta tgctcctgct ttcactattc aacacgtttt
     1441 tgacttatac taggctcatt tccaaaagca ttatatgata gtgatatgaa accaactaaa
     1501 ctaaacaaga aatataagta ataaaaactc gtttaaa
//
LOCUS       DAVLSL010000219         1527 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVLSL010000219 DAVLSL010000000
VERSION     DAVLSL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.221"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(182..943)
                     /locus_tag="VXN30_002047"
     CDS             complement(182..943)
                     /locus_tag="VXN30_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1358590.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
     gene            complement(943..1419)
                     /locus_tag="VXN30_002048"
     CDS             complement(943..1419)
                     /locus_tag="VXN30_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358591.1"
                     /translation="MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
BASE COUNT          456 a          304 c          210 g          557 t
ORIGIN      
        1 tatatttcgt tgcagtccaa ttagaaaata gcttgtaatc ataagaaaca ggtgtatgca
       61 aatgaattaa agtcttgtga aagctaccga atttctcttt ggaatttgtt agttcttgat
      121 aaacagactt tatcttgtta ttaaaccctc tcatttctat tctcctaaaa atatcaattg
      181 tttatttttc aagtaattca ttaagtctct tttcccccac ctgcaacatc tccttctcta
      241 cctgactcca ctccccaaag agtaactctt gaagaacatc tgctgctgaa gttgtatttt
      301 cttttgaatc atatacacaa cttctatctc gttggtaaat ttgaatgctt tcaaagatag
      361 ccagttcttc caattgtcgt gtattatcaa ctagatgatt tacaatgaaa tcatgatgtt
      421 cttttggagt tgcgcgtgct tgatttggat tgataatgta cagttcttca taacggataa
      481 gagtacttag atacgacaat tcaggctttg tcgcaattaa ggccaattgt acttcatatc
      541 ccttattttt caagagttgt gctgttttct ttggaacatc aactgttcgt aaagttccct
      601 cgatcaaaag attgtatctc aaactactca attttgttac taaagactct accatttttc
      661 ctgcaaaatc tttggtatat tctacactgt ctttgccata ttcttgctgc agttctaaat
      721 agtgtggatg ctgagaacga aaactatcac catctatgat aacaatattt ccttgaaatt
      781 ctttctgttt aatacgatga attgtagtct taccggcacc actttgccct ccaagcaaaa
      841 tcgctatagg ttgcttactg gacttttttc ctcttgtcag tgaacgaaga ttccttgcta
      901 aagcatgttt gaattcacta tcagtataat cttggatttc cattaagcta ccatccgctt
      961 ttcagatatc tctaacatac gttcaattcc atccaaatag ccactatatc tctctatttc
     1021 atcaaaagtt tctactaaat agatattcgt atgaatcaag tcagatagat catcactcat
     1081 taaaatccaa ggattagatt catcatcaat gctaattccc tgactatctt gataacgata
     1141 gagtcgagat aatagattag cacctctttc tttcacaatt tcaattttca aagtcaattc
     1201 ataatcttca acaggattga gcattttatc ttctcctaca atatcgacat aagatacatt
     1261 aaacttctga caaatgatgt ctattagttc cgtagagact gaactagttc cattttcata
     1321 ccggctcaag ctatttcgag aaattcctac aattcgggaa aagtcgtctt gtgttaagtc
     1381 atgtgtttta cgtaaggatt ttatgttctt tccaatcata gcaaactcct ttatttttat
     1441 aaatccatta tagcataaaa aagcataacg caccaaaaat ggtgcgttat ttcttgtttt
     1501 tctatcctaa actttacttc tataaaa
//
LOCUS       DAVLSL010000220         1496 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVLSL010000220 DAVLSL010000000
VERSION     DAVLSL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.222"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            24..1370
                     /gene="gdhA"
                     /locus_tag="VXN30_002049"
     CDS             24..1370
                     /gene="gdhA"
                     /locus_tag="VXN30_002049"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1358592.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHGGSIWTYAGNYDIALPCATQNEINGEAAKR
                     LVDQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVVNAMIAQGIV"
BASE COUNT          447 a          288 c          326 g          435 t
ORIGIN      
        1 tatgacaata aaaggagaac gatatgacat ctgctaaaga atatatccaa agcgtgtttg
       61 aaactgtgaa agctcgtaac gggcacgagg ctgaattcct ccaagctgtt gaagaatttt
      121 tcaacacttt ggaacctgta tttgaaaaac accctgagta tatcgaagaa aatatcttgg
      181 cacgtattac tgagcctgag cgcgtggttt ctttccgtgt tccttgggtt gaccgtgatg
      241 gaaaaattca agtaaaccgt ggttaccgtg ttcaattcaa ctcagctgtt ggaccataca
      301 aaggtggact tcgtttccac ccaactgtaa accaagggat cttgaaattc ctcggatttg
      361 aacaaatctt taaaaatgtc ttgactggac ttcctatcgg tggaggtaaa ggtggatcag
      421 acttcgatcc taaaggtaaa acagatgctg aagtgatgcg cttctgccaa agcttcatga
      481 ctgaattgca aaaacacatc ggaccatcac ttgacgtacc tgctggtgat atcggtgttg
      541 gtggacgtga aattggttac ctttacggtc aatacaaacg tcttaaccaa tttgatgctg
      601 gtgtcttgac tggtaaacct cttggatttg gtggtagctt gattcgtcca gaagcaactg
      661 gttacggttt ggtttactat actgaagaaa tgctcaaagc taacggtaac agctttgctg
      721 gtaagaaagt cgttatttca ggttctggta acgttgctca atatgctctt caaaaagcaa
      781 ctgaactcgg tgcaactgtt atttctgttt ctgactcaaa tggttatgtc atcgatgaaa
      841 atggtatcga cttcgatctt ttggttgatg ttaaagaaaa acgtcgtgct cgtttgactg
      901 agtatgcagc tgaaaaagca actgctactt atcatggagg ttctatatgg acttacgctg
      961 gtaactatga tatcgctctt ccatgtgcga ctcaaaacga aatcaacggt gaagcagcta
     1021 aacgtttggt tgatcaaggc gttatctgtg tatctgaagg tgccaacatg ccaagcgacc
     1081 ttgatgccat caaagtttac aaagaaaatg gtatcttcta cggacctgcc aaagctgcca
     1141 acgctggtgg tgtagccgtt tcagcccttg aaatgagcca aaatagtctt cgcctctcat
     1201 ggactcgtga agaagttgat ggccgtctca aagacatcat gacaaacatc ttcaacacag
     1261 ctaaaacgac ttcagaaaca tatggtcttg ataaagacta ccttgcagga gcaaacattg
     1321 ctgcctttga aaatgtagta aacgctatga ttgcccaagg tattgtttaa gatttatttg
     1381 cttaatcctc aatcactacg attggggatt tttagttgag ttagacagtt gggaaattct
     1441 aattaaatga aataaaaact gaacaaattg attgcggaat tcaaaccaat ttctaa
//
LOCUS       DAVLSL010000221         1473 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVLSL010000221 DAVLSL010000000
VERSION     DAVLSL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.223"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            14..1405
                     /locus_tag="VXN30_002050"
     CDS             14..1405
                     /locus_tag="VXN30_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1358593.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT          359 a          275 c          321 g          518 t
ORIGIN      
        1 tttggagtca aagatgaata tatttagaac aaagaatgtt agtttagata aaacagagat
       61 gcataggcat ttgaagttat gggatttgat tttgctgggt atcggagcca tggtagggac
      121 aggcgtcttt acaatcacag gtactgcagc tgcaacactt gctggcccag ccctagtgat
      181 ttcaatcgtt atttctgcct tgtgtgtggg attatcagcc ctcttttttg cagaatttgc
      241 ctcgcgagta cccgctacag gaggtgccta tagttacctc tatgctatct taggagaatt
      301 ccctgcctgg ttggctggtt ggttaaccat gatggagttc atgacagcca tatcaggcgt
      361 agcttcgggt tgggcagctt attttaaagg cttgctctct caatacggga tagcctttcc
      421 tcaggcttta aatggtacct ttaatcctca agcagggaca tttgttgatt tattgcctat
      481 tcttgtcttg gtcttggtga cttcgcttgt tttactaaat gccaaggcag ccttacgctt
      541 taattcgatt ctagttattt tgaaattttc cgctttagct ctctttgttt tagtaggaat
      601 ttggaatatt aagtttgata attggagcaa ttttgctcct tacggttttg gacaaatcta
      661 tggagctagc actggtatta tggctggtgc gtccttgatg ttcttcggtt ttttgggatt
      721 tgaatccatc tctatggctg tagatgaagt caagactcct caaaaaaata ttcctagagg
      781 gattgtctta tcgctttcta tcgtaaccat tctctatgcc ttggtgaccc ttgttttgac
      841 tggtgtggtt cactatagtc atttaaatgt cgatgatgcc gttgcctttg cccttcgtag
      901 tgttgggatt agttgggcag ccaactatgt gtcattagtg gctatcttga ccttgattac
      961 agtttgtatt tcgatgacct atgccctatc gcgtatgatt tacagtttag cacgtgacgg
     1021 cttagtgcct gctgccttta aggaactgac gaagactagc aagataccaa agaatgctac
     1081 tatcttgaca ggtctagctt cagcagtagc agcaggaatg ttcccactag ccagtattgc
     1141 agccttttta aatatttgta ccttagccta cttgatcatg ttggcttatg gtctgattcg
     1201 cttacggaaa gaaaaaggaa tgcccaaagc tggggaattt aaaacaccat tggtacctct
     1261 gttaccaatt ttatcgatca tcatttgttt atccttcatg ttacagtata atatgaatac
     1321 ctggattgct ttcctagttg cgttgttagt aggaagtatt atttacttca cttatggcta
     1381 taagcattct accatagaag aataaagtga aagtctattg agttgttgac actcagcaga
     1441 tttttatatg tgaaagaaat ttcaaatttt ttc
//
LOCUS       DAVLSL010000222         1458 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVLSL010000222 DAVLSL010000000
VERSION     DAVLSL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.224"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            98..1276
                     /locus_tag="VXN30_002051"
     CDS             98..1276
                     /locus_tag="VXN30_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1358594.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQIILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          543 a          262 c          212 g          441 t
ORIGIN      
        1 gtaagtaatg aaaagcttaa aaagattaac atctacattt atccatagca aaaaatcata
       61 ggattttaag aatctgacaa ctaggaagaa aactaatatg accttatcta agaaacaatt
      121 acaactaaga gctaaaatac tggaaacagt ttacacatta ggtcccattt cacgaattga
      181 aatcgctact aaaacaggaa taacacctgc aacaactagc agtatcacaa atgatctgat
      241 taaagaaaat attctcttag aacttggtga agacgaacac gatacaagcg tagggagaaa
      301 aaaaatcctg ctagacattc aagctaaacg attttactac attggttgtg aactatctga
      361 aaaacatttc acttttgctt taggagataa tctaggaaat atcttaaagg aagagaaaga
      421 aattgttact aaacagttaa tccaagaaaa gggaaatcaa ctcattaacc agacactaaa
      481 gcaattctta aataactgct ctgattatga aatagaagca attggaatcg cgctacctgg
      541 tcgctattta gataattata aaataacaac aaacaatcct ttatggcaac atatcgactt
      601 agaaatgatt caatcccact ttgataagcc tttgtttttc tctaacaatg ttaactgtat
      661 ggctataggt aaacgcctat ttagtcgcca acagaatgac cctaattttg cttattttca
      721 ttttgctaga ggtatgcact gttcttatat ctacgacgga aacatttatg ggaaaggaaa
      781 tctaatgatt ggagaaatag ggcatacagt tgtttcatct gagggagaag aatgtagctg
      841 cgggcgcaag ggatgcttac aaacttttgc tggagaatct tggttaatca aaaaatcaaa
      901 aattctatat catcaatctc cctattctct acttcctagt ttagtcaaaa atgccgatga
      961 tattgatatc caaattattt taaccgctta tcaattaggt gacactggaa taatcacact
     1021 tatccatcaa gccttactct atcttagtca aaccatttta aatatcagta tgatgattga
     1081 ctctcaaaaa atctacctac atagtccttt actaacaaat caacacatca tccaaaaact
     1141 atatagtgaa atgaactaca aacccaaact gctctataat cgcctacctg aagtcattat
     1201 tgaaccttac aatgatttta cagccgcaca ctcagctatt gcactttgtt tgtatcatac
     1261 aattctacat tcttaatttt ctattacatt taataaatag gctaaaaaga gggagtatat
     1321 cataaaaagc gaataaacct gttttctgaa aatcagaatt ctgttttgtt cgctttcttt
     1381 atatttggaa ttatactcct atctcccctt tttcataatg tataataaaa tgaaataata
     1441 acaggacgaa tcgatcgg
//
LOCUS       DAVLSL010000223         1458 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVLSL010000223 DAVLSL010000000
VERSION     DAVLSL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.225"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            131..>1458
                     /locus_tag="VXN30_002052"
     CDS             131..>1458
                     /locus_tag="VXN30_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEV1358595.1"
                     /translation="MNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPN
                     ELHKLIEKIAPKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGD
                     FSKKNIVLKEDDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNT
                     FKLDAKNAYFYTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLE
                     VLSENRSQDVNNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQ
                     LRFEIDGHEELRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKA
                     KFRELANIAFRQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQIIN
                     SVTGDEKKIIEFFRDSILNFNFLYRKLETINELFDEIREEHESYRRGGWSA"
BASE COUNT          551 a          170 c          263 g          474 t
ORIGIN      
        1 taatttatct ataactcaaa taaagaattc tattaaagaa aattccttag ttttgtttgt
       61 tggtgcagga atatcagcta attctaatct accaacttgg ggagaattga ttcaatcatt
      121 aaaaaaagaa ttgaacatac cagaagaaag aacagatagt ccactgagaa ttgctcagta
      181 ttattatgat acttttggga aaaatcagta cacaaagaag atagaagaaa ttttctttaa
      241 gaagggattg agtaaaccaa atgagttaca taaattaata gaaaaaattg caccaaaaca
      301 tattattacg acaaactatg attcacttct ggagagtcaa tttgaaagtg gcttgttaaa
      361 gtacaatgta gtcgctgagg ataaagacat cccttatacg agttcggaaa gatacttgat
      421 taaaatgcat ggtgacttca gtaagaaaaa tattgttttg aaagaagatg attatttaga
      481 ctatcatttg aacttcccaa tgatttctac tttaatccag tcattgataa tgaatcatac
      541 tctattgttt gtggggtatt ctctaagtga ttcgactttt aattcaattt ttagaatgat
      601 tcaaaataca tttaaattgg atgctaaaaa tgcttatttt tatactcccg aggaaccgtc
      661 tatgatcatt cgagagtatt ataagaagca aggaattttc attatctcta atgaagagaa
      721 tctaggtcag gagacatcag aaaaacagaa caaattgtat tgcaggacaa aagatttttt
      781 agaggttcta tcagaaaacc gaagtcaaga tgtaaataat gctgatgatt tatggaatca
      841 actggctttt ttagatagac ttagttttat tgatgcgaaa gatttttcta gatattctga
      901 tttaaagaaa agggctttaa actgggatga tgagtattgc tggtttggaa atgatcaatt
      961 aagatttgaa attgatggtc atgaagaatt acgaattatg gtatctaaaa aatctttatt
     1021 aaatcgcttt ctagatatgg agattggtga gcctagggat ttaaagggga atcgattctt
     1081 aagtaaggct tttaagttgt atgaagaaaa acaatattct ttagcaaaag caaaatttag
     1141 agaactagct aatatagcat ttcgtcagaa agactatttt aatttcttag tttgtgaatt
     1201 taattttcag caaattcaaa taattgatag atacgaaaaa acacctagtt atgcagagcc
     1261 actttatgat ggagaattaa gcgaattaac agaacaaatt attaactcgg ttacaggaga
     1321 tgagaaaaag attatagaat tttttagaga ttcaatcctt aacttcaatt ttttatatag
     1381 aaaattagaa actattaatg aattatttga tgaaattcgt gaagagcatg aaagctatag
     1441 aagaggagga tggtctgc
//
LOCUS       DAVLSL010000224         1448 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVLSL010000224 DAVLSL010000000
VERSION     DAVLSL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.226"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            18..>1448
                     /locus_tag="VXN30_002053"
     CDS             18..>1448
                     /locus_tag="VXN30_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEV1358596.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIG"
BASE COUNT          522 a          204 c          303 g          419 t
ORIGIN      
        1 tataagagag gttgctgatg acaatagata ttagtgaaga aagtttggct aaagaatcgg
       61 ctgacttatt aaaaattctt ttaaaagatc gaacgacaaa gaaatcaatt gtttgggcta
      121 cgcattctta tgaattgtta ggaaagggat ttgctccaag tgatcgcatc aatccaagta
      181 aggttactgg aaactttgcc aacttgattc agcccagatc tgaaaaatcc aaatatgaac
      241 aaaaagatcg gacaaaaata agggcagaag tgtttacacc aacgtggttg gtcgcaaagc
      301 aaaatggata tgtagaatcg aaattaggct cgttaagtct tgaggagtat gtggacttac
      361 gatggttaga ggtaacttgt ggtgaagcac cttatatggt aacgagatat gataccgtaa
      421 ctggtgagga aattccatta tctgagcgag ttggttttgt tgatagaaaa ctgcaacgga
      481 ttagtcgtga ggtttcagat gaagtcacat tttataaact tgtaaaagat gcttatcatg
      541 cttcgtatgg ttatgagtat caaggagatt ctctcctttt ggcgcgtgag aatcttttgg
      601 caacttttga ggactattat ctagctaaga ctggaaatca accaacatta gagcaaaaga
      661 aagaaattgc gactattatc tcttacaatg tctttcagat ggatggattg aaaaaaagta
      721 gtccttattc agctaaacaa aagcaatctc aacaattgag tttgtttgcg gatgagctag
      781 aagtccaaga agtagaagaa tctaaaaccc atatcaaaga ctggaaaaag aatagaatga
      841 taggttttga gcgcctatct agcgaggaaa gtgaaatgaa atttgatgtc gtgataggaa
      901 atcctccgta tcaggaaaca ggagaagcca gagatgagcc gatatatcac tattttattg
      961 atgaagctta taaaatcgca gataaatcta tactaatcac cccagcaagg tttttattta
     1021 aagctggtca aactcctaaa aattggatgg aaaagatgct tgaagacaag catttaaaag
     1081 ttcaattcta tgaacttaaa agcggtaaag tatttacagg aacggatatt aagggtggag
     1141 tagcaataac ttatcgtgat gttgataaag atttgggacc aatcggagta tttactattt
     1201 ttgaaacttt gaatggtatt gtaaaaaaaa tcgaaaaaca tccagaattt gaaacttttt
     1261 ctgatttagt ttatcctcaa ggtatttata gattttcaaa taaacttttt tctgattatc
     1321 caaatgctga aaagctgcaa ggaaaaggga caaaaaataa aattgtttcg aaatcactaa
     1381 ctcagttaga ttttgttttc aaaaacaacg aagaaaaaga ccatgttgct ctacttggaa
     1441 gaattggc
//
LOCUS       DAVLSL010000225         1438 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVLSL010000225 DAVLSL010000000
VERSION     DAVLSL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.227"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            31..216
                     /locus_tag="VXN30_002054"
     CDS             31..216
                     /locus_tag="VXN30_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1358597.1"
                     /translation="MIYDKIKEIASEKGISIYRIEKDLDLGNGAISKWNNSSPSATTL
                     NSIANYLNVRLEQLLEE"
     gene            218..934
                     /locus_tag="VXN30_002055"
     CDS             218..934
                     /locus_tag="VXN30_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEV1358598.1"
                     /translation="MNELINVTLNDNQEPVVSGRQLHEALGVNSRYTTWFDRMKEYGF
                     TEGQDFLPNLGKSTGGRQATDHIIKLDMAKEIAMIQRTERGKQVRQYFIQVEKDFNSP
                     EKIMARALLMADQKVHKLEAQIEADKPKVLFADAVSASHTSILVGELAKLISQNGYKI
                     GANRLFSWMRENGYLIKRKGSDWNMPTQRSMDLKLFEIKETNVQHADGHITVNKTPKV
                     TGKGQQYFINKFLNQEYLTG"
     gene            1102..>1438
                     /locus_tag="VXN30_002056"
     CDS             1102..>1438
                     /locus_tag="VXN30_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEV1358599.1"
                     /translation="MMVVLANHPNWQVYPDEIAKRKGVNRKTIDKYFKIFEEAGYLRK
                     IRKKPPGNGGSHIFRFFSDVKISDFQFDIMKQRLNLSIKRASMDYNSDIPKSEMSESE
                     MSESEMSESE"
BASE COUNT          517 a          251 c          319 g          351 t
ORIGIN      
        1 aaaattttct atcaaaagga ggtgccggaa gtgatttatg acaaaataaa agaaattgct
       61 tcagagaagg ggatttcgat ttatagaatc gaaaaagatc ttgatttagg taacggggca
      121 atcagcaaat ggaacaacag ttcgccatct gccactactc taaattcaat tgcaaattat
      181 ttaaatgttc gtcttgaaca attgctggag gaataacatg aacgaactca tcaacgtaac
      241 cctgaatgac aatcaggagc cagtagtgtc gggaagacaa ctacatgagg cgctgggtgt
      301 taattcaaga tatacaacat ggtttgaccg tatgaaggaa tacggattta cagaaggtca
      361 ggactttctc ccaaatttgg gaaaaagtac aggagggcga caagctactg accacatcat
      421 caagctagac atggccaagg aaattgctat gatccagcgg acggagagag gcaagcaagt
      481 ccgacaatac tttatccaag tagaaaaaga ctttaatagc cctgagaaga ttatggcaag
      541 agcattgctc atggctgatc agaaagtcca caagctggag gctcagattg aagcggacaa
      601 acccaaagtc ctctttgcag acgcagtaag tgcaagccat acatctatct tggttggcga
      661 acttgccaag ctcattagcc aaaacggcta caaaattgga gccaatcgcc tcttttcttg
      721 gatgcgcgaa aatggctacc tgattaagcg caaaggttca gattggaaca tgccaaccca
      781 acgtagcatg gacttgaaac tctttgaaat caaggaaaca aacgtgcaac acgcagatgg
      841 acatatcact gtgaacaaga caccaaaggt cacaggcaaa ggacaacagt attttatcaa
      901 taagttcctt aatcaggaat atctgacagg ttagaaagta gaaagaatga ttgaaaataa
      961 gtgaagagaa aggagactgt atgacagact ttaaaaattt agattgtcaa tttatctttc
     1021 aagaatgcga ctgaaaatta tactgctgtt agtaatagtt ttatcaacga tcctgcgctg
     1081 gattttacag cggttggcat catgatggtg gtgctggcta atcacccaaa ttggcaagtc
     1141 tatccggatg agatagctaa aagaaaaggt gttaaccgaa agacaatcga taagtatttc
     1201 aaaatctttg aagaggctgg atatttacga aaaatcagaa aaaaacctcc tggaaatgga
     1261 gggagtcata tattcagatt cttttcagat gtaaaaatat ctgatttcca attcgatatt
     1321 atgaaacaga gattgaacct atctatcaaa agggcgtcta tggattataa ttctgacatt
     1381 ccaaaaagtg agatgtcaga aagtgagatg tcagaaagtg agatgtcaga aagtgaga
//
LOCUS       DAVLSL010000226         1404 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVLSL010000226 DAVLSL010000000
VERSION     DAVLSL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.228"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(106..1302)
                     /gene="tuf"
                     /locus_tag="VXN30_002057"
     CDS             complement(106..1302)
                     /gene="tuf"
                     /locus_tag="VXN30_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1358600.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          377 a          286 c          304 g          437 t
ORIGIN      
        1 tgattttcat tgagtattag ttcaatatct aaaaaaagaa ccttgccaag caagattctt
       61 ttagtgtctg acttaaataa ttgttcttct gggaactaaa tcgaattaag cttcgatttc
      121 tgtaaccata cctgaaccaa cagtacgtcc accctcacgg atagagaatg tagtaccttg
      181 ttctacggcg attgggtgaa tcaactcaac gtcgattgtc acgttatcac caggcattac
      241 catttcagta cctgctggaa gttcgattga acctgtaacg tcagtagtac ggaagtagaa
      301 ttgtggacgg tagttgttga agaatggagt gtgacgtcca ccttcttctt tagtaaggat
      361 gtagacttca cctttgaatt tagtgtgtgg gttgattgaa cctggtttag cgataacttg
      421 tccacgttcg atttcatcac gttgaacacc acgaagaagg acacctacgt tatctccagc
      481 aagaccttcg tcaagttgtt tacggaacat ttcaacacca gtaacaactg ctttttgagt
      541 ttcttctttg ataccaacga tttcgatttc gtcgttgact ttaacgatac cacggtcgat
      601 acgtcctgaa gcaactgtac cacgtccagt gattgagaat acgtcctcga ctggaagaag
      661 caatggtttg tcagtgtcac gttctggttc tgggatatac tcatcaactg tgttcatcaa
      721 ttccataacg atgtcttcgt atttagagtc accttcaaga gctttaagtg ctgaaccttg
      781 gataactgga agatcgtcac ctgggaagtc gtattctgac aataggtcac ggatttccat
      841 ttcaaccaat tcaagcaatt cttcgtcgtc aaccaagtca actttgttca tgaagacgat
      901 aaggtgttta acaccaacct gacgtgaaag aaggatgtgc tcacgagttt gtggcattgg
      961 tccgtcagtt gaagctacta caaggatagc tccgtccatt tgagcagcac cagtgatcat
     1021 gtttttaacg tagtccgcgt gtcctggagc gtcgatgtga gcgtagtgac gtttttcagt
     1081 ttcgtactca acgtgcgcag tgttgatagt gataccgcgt tcgcgttctt ctggagcagc
     1141 atcgatagac gcatagtctt taggttggtt aactgatgaa ggcaagcgac gtgccaaaac
     1201 agttgtgata gctgcagtta gggtagtttt accgtggtca acgtgtccga tagtaccaat
     1261 gttaacgtgt ggtttactac gatcgtattt ttcttttgcc atttgagtaa aagcctccaa
     1321 taaaatatat tttatagata gacagtaggc aatacagtct aactttcctt actattttat
     1381 caaatttcag ctaaattgca agtg
//
LOCUS       DAVLSL010000227         1395 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVLSL010000227 DAVLSL010000000
VERSION     DAVLSL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.229"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(30..794)
                     /locus_tag="VXN30_002058"
     CDS             complement(30..794)
                     /locus_tag="VXN30_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358601.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(890..1293)
                     /locus_tag="VXN30_002059"
                     /pseudo
     CDS             complement(890..1293)
                     /locus_tag="VXN30_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          531 a          276 c          209 g          379 t
ORIGIN      
        1 ttttttgtaa aattgaacaa attgtttcat tatacgtagt cctctgaatg tagaaaaaat
       61 gtaccataaa caaccaaaca actaacaaat aaaataaaag caagatgccc actaactaag
      121 gaaagactga tatctttctg atatcccaaa gctaatgttg tcacaggttc taagtaagat
      181 agccctaaaa tagcccaaaa aataccacca accatcatat aggcaactgg gatgaaaata
      241 gctcctattt ttttcttcac tagcaaagca ctagctagtc caaaaataga gaacacagcg
      301 ccccaaactc cataccagag agtcgttaca agactataga gaaactgatt ggaatcaaat
      361 aattctttta aggcaccact ataatctcca atataaattt cctgataagg agtcactaaa
      421 agattaattc ctaataataa taaatagggg agaaaaaaga ctagaaaaga agaaataatt
      481 acagtactac ccacaatagc cagatacttc tttttagaaa ttcgacacaa ttgtgctgtt
      541 agaaaatgac tctcagcatc ctctattatc tgactagaat agggcagtgt acagataagt
      601 gcagctacta ggctaatcgg tgaaaatcct cgaatagaag aagcggcaaa aaaaatcgat
      661 aaaccttcaa ttttataaat accattgaaa gcaaggaaat ttcctaaact catgcaaaga
      721 agggtcaaaa ataaagacat ataaaatcga ggtgattgaa cgactcgtac aagattacaa
      781 atgaaaaatt ccatccttac tcctccttat aataaaaata gggtgtagca ttcttttttc
      841 atgctacacc cacaatcaac catctctaag gcttactctg acaagtaagt taataagaat
      901 ctggattcca agaacctgaa gtatgaattc ttacatgatt tccaaattgt ggcgccatag
      961 ctaatctagt accagaacca atataattgt caccacctcc attatagtac atgacaatcc
     1021 tagagccaga ccccaatgaa tataccgggg taatatctga cccactatag gcgctacgaa
     1081 tagaggtatt taacctttta ccgccaccag tgctgtcatt attattaatt ccagcagagg
     1141 cgttttcttt ctcaagctca ctagttgtat ccgaacccca acgcggcaac gttaaatcat
     1201 aatctccaat ctgtctagct aaaactgtag ggagaaacaa actagatgca attgtaacta
     1261 cgcaaaaaag gaatatattt ttcttctttt catgacatcg tcctcttaag aagtgataaa
     1321 caagatagtt gattgatcaa tttgatacat attacatttt cattatatca ataaaagaaa
     1381 gcgttgtcaa ttatt
//
LOCUS       DAVLSL010000228         1380 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVLSL010000228 DAVLSL010000000
VERSION     DAVLSL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.230"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            67..348
                     /locus_tag="VXN30_002060"
                     /pseudo
     CDS             67..348
                     /locus_tag="VXN30_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1089..1349)
                     /locus_tag="VXN30_002061"
     CDS             complement(1089..1349)
                     /locus_tag="VXN30_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358602.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT          463 a          259 c          235 g          423 t
ORIGIN      
        1 ttgttagtgt tcagcgtacc acttttgaag agatgttagc tgtattaaaa acagcttatc
       61 aaaaaagtcg gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag
      121 atatatcctc aagcacaaac tccacgtaaa tccagcaaac tcaagccgct aatagctgaa
      181 gataaagcct ataaccatgc gctatctaag gagagaagca aggttgagaa catctttgcc
      241 aaagtagaaa tgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgcttcgga
      301 ttacgaatga atttgattgc tggtattatc aatcatgaac taggattttg gtttcgcagg
      361 aagtctatta tatatgcaca gacatatgaa atctttttga gacagcagtt atgatatatt
      421 tatccattca caacatcttc tgtactaata ctacaatatt ggtacatttt gattacacaa
      481 tcgtttcaaa aaatatgatg gaagcctgag tattttatag taattccctt cctatcaact
      541 agatcaacca gttgagtgtt gaatgttgaa acaccctagc ctcagcaaag gactatgatc
      601 agcatttcct agccaatagg acgctccaaa tcacctattc taagtccatc actagcttgg
      661 tcagtaagat acagactgca aggcgagact gacgtggttg atgagatttt ggaagagtat
      721 tacaatcata gattcaccag tagacttgac cgcagattta actatagtag attgaaacta
      781 gaatagtaca tctctgcttc taaaacattg ttagaaatcg atttgactgt cctgatcaat
      841 ttgtcctgtt ctcgtttcat ttcactatac taaaaaacca cctgaaacaa atcaagtgac
      901 tagaggataa aaacaaagac taggcaaaaa gttttgaaaa gcaaaaaacg cataatatca
      961 ggtcttgaaa aaccttgata ctatacgttt gattgtggga agattgactt cattttctcc
     1021 tgaaattgaa gttttttcta gtttccagat atagacgaat tcaatatagc catctcgcta
     1081 caattatttt aaccatcgag aaaatttttc agaatttcaa aaatttcatt ttgatccaat
     1141 gaaatgtcat cattttgaag aatagttgac ttcacaggct ctaattctat cacaaagcgg
     1201 tgtatgtctg cttttaccgc actacttgat gttatagccg ataacttttc atctcctagc
     1261 aaggaagctg tcaaacgggt aatgtcattc aaatgctttt taatggactt acttaaacct
     1321 tgagcacctg tagcagagcg ttccctcata tccagccaag ctttagcctt aaaaataatt
//
LOCUS       DAVLSL010000229         1355 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVLSL010000229 DAVLSL010000000
VERSION     DAVLSL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.231"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            31..870
                     /locus_tag="VXN30_002062"
     CDS             31..870
                     /locus_tag="VXN30_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1358603.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            977..1252
                     /locus_tag="VXN30_002063"
     CDS             977..1252
                     /locus_tag="VXN30_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1358604.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          447 a          211 c          291 g          406 t
ORIGIN      
        1 tctgaatcct aaaatagaaa gattgatact atgacaaaaa ttaagattgt aaccgattca
       61 tctgttacta ttgaacctga actagtaaag caattagata ttacaattgt tccattatct
      121 gtaatgattg ataatgttgt ttattctgat gcggatttga aagaagaagg taaatttctt
      181 cagttgatgc aagaaagtaa gaatcttccg aaaacaagtc agccacctgt aggtgtcttt
      241 gctgagattt ttgaagacct atgcaaagat ggtggccaga ttcttgctat tcatatgtcc
      301 catgctcttt cgggtacggt agaagcagca cgccaaggtg ctagcctatc tactgcagat
      361 gtgactgttg ttgatagttc cttcactgac caagccctga aattccaagt tgttgaggct
      421 gcgaagttag ctcaagaagg taaagatatg gaggcaattt tatctcatgt agaagaggtt
      481 aaaaaccata cagagcttta tattggtgtt tcaactttgg aaaatcttgt caaaggtgga
      541 cgaattagcc gtgtaactgg cttgttgagc tctcttctca atatccgtgt tgtcatgcaa
      601 atgaaagacc atgaattgca gccaatggtt aaaggtcgtg gaactaaaac atttaaaaaa
      661 tggttagatg agttgataac atcgctttct gaacgtgctg tagcagagat tggaatttca
      721 tattctggta gtgatgattg ggcaaaagag atgaaagaaa gcttacaagc ttatgttgaa
      781 aagccaattt ctgttttgga aacgggttcc attattcaaa ctcacacggg tgagaacgct
      841 tgggctattt tgatacgtta ccactcctaa aaaaataaat aaaatgggaa gaaatagcgt
      901 ttttaacttg acctaaaagg gattttagga tatgattata tttgttaatt agaaattatt
      961 tggaggaatc attaacatgg caaacaaaca agatttgatc gctaaagtag cagaagctac
     1021 agaattgact aagaaagact cagcagcagc agttgaagct gtatttgcag cagtagctga
     1081 ctatcttgca gctggtgaaa aagttcagtt gatcggtttt ggtaactttg aagttcgtga
     1141 gcgcgcagaa cgtaaaggtc gcaacccaca aactggtaaa gaaatgacaa ttgcagcttc
     1201 taaagtacca gcattcaaag ctggtaaagc tcttaaagac gctgttaaat aatcagtctt
     1261 taaaaagcct attgtatcaa gctttctagc ttggtcagta ggctttttaa ttatgaaatc
     1321 tagtaataaa tagagttaaa aagttgaatg ttccg
//
LOCUS       DAVLSL010000230         1315 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVLSL010000230 DAVLSL010000000
VERSION     DAVLSL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.232"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..248
                     /locus_tag="VXN30_002064"
     CDS             <1..248
                     /locus_tag="VXN30_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358605.1"
                     /translation="GIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVV
                     INEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            563..>1315
                     /locus_tag="VXN30_002065"
     CDS             563..>1315
                     /locus_tag="VXN30_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358606.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINNPISLYGMLD"
BASE COUNT          439 a          178 c          220 g          478 t
ORIGIN      
        1 tgggaatcat ttacgataat gaagcattaa gagatttgtc aaaacaatat ttaaacaaaa
       61 atatttcgga attacaagat ggttctttcg agggcggtat acaagatgct gttgcctcta
      121 aacaaatagt agttataaat gaatttatag cttgtttaaa ttccagatgc gtgccatccc
      181 aatatgatag atttttttat aaagatagac cttatatttt ttccagaaag agtcctatta
      241 aggggtgaaa atttgttaca tttggattaa caagtgtcct agggattaga aataactcta
      301 gtatagctag atatttttga gtaactagaa atatcattag gctacccata tgattgccag
      361 aaaagatggc tgatgaatct tatttatact gttgcagtag gcattgtcta ggataaataa
      421 gctatcttga tgggggaaat tggaataatt ttagtagaaa cattaaagga ttatggataa
      481 gtagcgcagg attaggtgat gcaatgtgct aagttggctt ggtgttagca gtagtattat
      541 ttaacaatgg aaagtagact aaatgaaaaa taaacgttat ttttttgaca caattttaat
      601 aattttactg cttataagca cgatattttg tgtcagccca gtatttataa aattggacat
      661 cttaggaact ccctctcatg ccatcttaac ctttgtattg gcgataccgt tattctatat
      721 tttgtctcaa tgcttacata ctttgctatt actagtttcc tcaatctttt gtaaattaag
      781 accaatttat ttttatttta tatttgtgat cattatagga gctaggaaat actatcgaat
      841 tctctttcac caactaatgg gatttagtcc aggaatagca gtgttttata aggagagtca
      901 aacaactaag aatctgttta aattttacta cttcttgtac tttacaacac taatttcata
      961 ctattttttc tttacttttg tgtatgacaa accgttgctt ttgcctctta tttcattttc
     1021 tattatcata gcattagtac aaaaacttta tagaattgag aatcaacaat tatttctgtt
     1081 aaaatcaaag gtactaacaa tactggaatc taagaagaat tgtgagttta atcttcagga
     1141 ttatcatgaa atttggaaat tgcaatcaaa atctgagttg ccatgcgtgg ctttatctta
     1201 tatcagttta atcaagccat atctttcaga gagtgtgcgt gaacaaattg atttattaga
     1261 agttaagcga tttaaaaaaa taaataatcc aatatcactt tatggtatgt tggat
//
LOCUS       DAVLSL010000231         1313 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVLSL010000231 DAVLSL010000000
VERSION     DAVLSL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.233"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..675
                     /locus_tag="VXN30_002066"
     CDS             <1..675
                     /locus_tag="VXN30_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1358607.1"
                     /translation="DKRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFI
                     KELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRY
                     RQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEY
                     KNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNY
                     DVGESL"
BASE COUNT          558 a          172 c          249 g          334 t
ORIGIN      
        1 gacaaaagga taaaaaggtt aaggagaata tctggaggag atacctatac gataatctac
       61 ctaaaacttc tactactaag cctaaaagat gagggcaaac tctattatga cggagtagaa
      121 agtgatttta taaaagagtt agcactaacc atagatgaaa cagacgatga tgtaatggta
      181 acagtaaatt atttaatcaa tcaaggctta ttagaagttg taacagaaaa tgatgaatac
      241 tacctaactg aaataccaaa cttaatcgga tcagaaacag cctgggcaga gaaaaaaaga
      301 agatacagac aaaataaaca gaggacattg tccttaatga gtccaaccca tgtccgacaa
      361 gagatagaga tagagaaaga tatagagata gataaagaga gagagggaga gatagataaa
      421 aagtccaccc ccatttttta tggagaattc aaaaatgtaa ggttaagcaa agaagaatat
      481 aaaaatctaa aagaaaaatt aaactcacac acagatataa tgataaataa actatccaga
      541 tacatggaaa gcagtggtaa gacctatcaa aaccactatg tgacaatctt aaaatggtat
      601 gaagaagata aagacaaact aagacagaaa ggtttaaata aaaaaatgaa ttatgatgta
      661 ggagaatctt tatagataaa aagaggtgga aaggcactat taaagacttt aagtttaaaa
      721 aggtatcaaa gtatgccaga gataaaaaaa tataaatatg acactttaaa ggcgattaga
      781 aaattaaaga aatgctagaa gacttttaga tgacagttac caagagggtc taattttcac
      841 tgctacttat gcagtaggaa ttggaccttt ttatataagc aaattctatt tcaaaattgg
      901 ttaccaagaa agacttcttt tcactgttaa gtataagagg gtaatttata gaattttgtt
      961 acatcaagat aaatattttc tgatggtcaa attttaaaaa gattaataac tttcgataaa
     1021 ttttgaacct taacaattga atagaccaag acaagacgtt aattattttt ggagcgtaat
     1081 aacctatccg tcaagatctt tcggctgaat aattcggcaa aaaaagttct gctaagcaaa
     1141 aagaaagtga gcgataagta agagtttgaa tatagggagg gataccctat ccgctatgaa
     1201 gaaaatgagg ataatgatac ttctaaggtt gaagccggct catccggaat agaggcggag
     1261 gtgagattcc tatgttgcta atccaaagca cttgtcaatg tcaaaaaact taa
//
LOCUS       DAVLSL010000232         1301 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVLSL010000232 DAVLSL010000000
VERSION     DAVLSL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.234"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            289..609
                     /locus_tag="VXN30_002067"
     CDS             289..609
                     /locus_tag="VXN30_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1358608.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            complement(662..1249)
                     /locus_tag="VXN30_002068"
     CDS             complement(662..1249)
                     /locus_tag="VXN30_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1358609.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
BASE COUNT          397 a          256 c          227 g          421 t
ORIGIN      
        1 tctacctaca tagtacctct tattaagctc atttcaaaat tacgttaact tttttcacga
       61 aaaggagaaa attatgcgtt atgattatgg atagcgcttt caaattttta aactcctatc
      121 ccgtcctttt atctatataa taagcataag cgaaaatata ataactgtca agtaactgaa
      181 gtgaatttta taaaaaaatt acaagccaaa tttgtaaagt ttacactaag ccgctagggc
      241 aatcgtctat cagaatgtcc gtttatttgt caataatccg agaaaatctt gcaacgctta
      301 gaagtctata aaaactatca acatttatat gacttgcgaa tgacaattct attaaacctt
      361 tccacactct atctatacaa tcaagataaa aacatgtgta agcaaatctg ctacacttta
      421 ctggaggacg ccaagaataa gaaaagctac gataggctgg ctatctgcta tgtccgtatt
      481 gggatttgta gggatgatgc gaaacttatc caaaaagggt tctcactcct agagctgacc
      541 gaggaaactt ctatgctgtc tcatctcaaa aaagaagtag agacccatta tcaaccaaag
      601 aaattataaa aaaagtcgag ggatttcctc gactctttac atattatttg ttcgttagtt
      661 cttaatacca gccattgtta agccagaagt ttttagcagc agtccatgaa ccgtaacgtc
      721 ctgcaacgta ggcatctgct acacgttctt ggttttcagc tgagtagtca ccgttcaggt
      781 atgaatctgt taattggtaa cgtccgatat aacgtccatt tgtagctgta tagctaccac
      841 ctgattcttt ttgagcgatc cattctttgg cttctgcttc agatccgctt acagttgaaa
      901 caactgtttc acttactact ggagtttctg ctactggagc tgaaacagtt tcatcttgag
      961 cggctggtgc cgcataagta gctggcgctg gtgttgcaac aggcgctaca gggccatcga
     1021 taaccaactc ttgatcaaca taaatcaaat gaatgttatc aatgtggttg ttttctgcca
     1081 atttttcaac tgttgtgttg tgagtttcag cgatttctga aagtgtatca ccttctttaa
     1141 cagtgtaagt tgatgattct tgagcagata caaatgatgg agcaaatact gcaaacaagg
     1201 cagctactcc tgcaagagtt gctttaatct ttttagttat tgatttcata ttcgaaaatt
     1261 ctccttcttt ctatagttct atcataccct ttaaatatta c
//
LOCUS       DAVLSL010000233         1283 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVLSL010000233 DAVLSL010000000
VERSION     DAVLSL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.235"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(147..335)
                     /locus_tag="VXN30_002069"
     CDS             complement(147..335)
                     /locus_tag="VXN30_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358610.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
     gene            complement(295..477)
                     /locus_tag="VXN30_002070"
     CDS             complement(295..477)
                     /locus_tag="VXN30_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358611.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(479..1258)
                     /locus_tag="VXN30_002071"
     CDS             complement(479..1258)
                     /locus_tag="VXN30_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358612.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT          414 a          278 c          196 g          395 t
ORIGIN      
        1 tatatactgc aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt ataaaatgat
       61 tttactgctt atattatacc atatggagag agattaaaaa actagcagtg gagttcctgc
      121 tagctctctg cgacttggat taccatctag ataagtttaa agagccaatc caatatctta
      181 aataatatct tgtaaaacag caattgaact gcaaaggaaa tgatcatcca aaagaatttc
      241 acaatcccaa taatcggttt gataaaaaga atagcaagaa gaccaaaaaa aatttcattc
      301 tctctcctct ggcctaatga gccaccgagc cgtatccatt agcttatctg caataaaaag
      361 ttttcccaga ccaacaacga catggtcagc tttctttatc gttttcatca cttttacacc
      421 ttgcacggtc aaaaagtaat taccataaat tttatcaaaa gctttgatag gattcatatt
      481 actctccttt aatgatttca gcctcttttc ggattgtttc aacatcttct tgatattgaa
      541 cttcactata gttgactagc ttggataatt ctttctgcaa gctttccccc attggtttca
      601 tagttgcaaa ggttaaacca cctgcaatga taccacccaa gataggaata aatttcccta
      661 ttcctttggc cagccctccc ttggtaagat tcacaccaaa tatttttaag acttttttca
      721 aaatagggta ccaaagcgtc tttgttaaag ctttattagg cactgttttc attacctgtt
      781 tggcaattgt tataccacca gcacatagca aagcagcggt tccattcacc cctaacatta
      841 cgcctagata aagcaagagg gtattttgag catcttcact caactcctcg cgtgaagccc
      901 aaagatcctc ataaccataa atataaccta attcttgagc caatttcaga gagaaagcat
      961 aaaattgagc cacatcagct ggaatggtaa ttgccatagc aagccctcca gctaatcctg
     1021 ccaaaacaga agtcccactc gctaataata cattgtcccg aatacaagca ttagctacac
     1081 gatctaatat ttcttgagat agaagagtcg ttgtaccttg ttccaataag gtaggaatat
     1141 cttttggatc caattctttg gaaaacttat ccactaaaaa tttcgaacga tcaaccttaa
     1201 caacaggtag tttcaccact tgttgcaata cttgcatagc taagtcttgt tcagccatat
     1261 acaaactcct tatttctttt tta
//
LOCUS       DAVLSL010000234         1278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVLSL010000234 DAVLSL010000000
VERSION     DAVLSL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.236"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>1278
                     /locus_tag="VXN30_002072"
     CDS             <1..>1278
                     /locus_tag="VXN30_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1358613.1"
                     /translation="EHKRWFHRSELAELLNCTERAVKDDLSHVKSAFPDLIFHSSTNG
                     IRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQAESICKEFYISSSSLYRITS
                     QINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSEKYYFLEWPFENFSSEPLSQL
                     LELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFMEVDKDSFNDQSLDFLMQAEGI
                     EGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESLFMKCVKKDSYVEKSYHLLSD
                     FIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEFILFDQKGNTIRNFQNIFPKF
                     VSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHLVINLLQNQPKLKVLVMSNFD
                     QYHAKFVAETLSYYCSNNFELEVWTELELSKESL"
BASE COUNT          422 a          185 c          209 g          462 t
ORIGIN      
        1 gaacataaac gttggtttca tcgttctgaa ctagcagagt tattaaattg tacagaacgt
       61 gcagtcaaag atgatctatc ccatgttaaa tctgcttttc ctgacttgat ttttcattct
      121 tctactaatg gtatacgcat tattaatacc gatgatagtg atattgaaat ggtttaccat
      181 catttcttta aacattcaac tcatttttcg attttagaat tcatcttctt taatgaaggt
      241 tgtcaagctg agagtatttg taaagaattt tatatcagtt catcttcgct ctatcgtatt
      301 actagccaaa tcaataaagt gattaaaagg caatttcaat ttgaagtcag tctgacccct
      361 gttcaaatca ttggaaatga gagagacatt cgttactttt ttgcacaata tttttcagaa
      421 aaatattatt tcctagaatg gccatttgaa aatttttcat cagagccact atctcaattg
      481 ttagaattgg tttataagga aacaagcttt ccaatgaatt tgtcaacgca tagaatgcta
      541 aagttgctcc tagttacgaa cctatataga ataaagtttg gtcattttat ggaagtagat
      601 aaagattctt ttaacgacca aagtttggat tttttaatgc aggcagaagg aatagaaggt
      661 gttgctcaga gttttgaatc agaatacaat atctctttag atgaagaagt tgtttgccaa
      721 ttatttgtgt cttactttca aaaaatgttt ttcatagatg aaagtctctt tatgaaatgc
      781 gtaaaaaagg atagttatgt cgaaaaatct taccatctat tgagtgattt tattgatcag
      841 atttcagtca agtatcagat tgaaatggag aataaggata atctgatttg gcatctgcat
      901 aataccgcac atctgtatcg tcaagagttg tttactgagt ttattttatt tgatcaaaaa
      961 gggaatacaa tcaggaactt tcaaaatatt tttccaaaat ttgtttcaga tataaaaaaa
     1021 gagctttctc actatttaga gactcttgag gtttgttcta gttcaatgat ggtaaatcat
     1081 ctatcttata ccttcatcac tcataccaaa catttggtga ttaatttgct acaaaatcag
     1141 cctaaactaa aggttttagt tatgagtaat tttgatcagt atcatgcaaa attcgttgca
     1201 gagacacttt cttattactg tagcaataat tttgaactgg aagtttggac tgaattagaa
     1261 ttatcaaagg aatcttta
//
LOCUS       DAVLSL010000235         1258 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVLSL010000235 DAVLSL010000000
VERSION     DAVLSL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.237"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            155..1180
                     /gene="trpS"
                     /locus_tag="VXN30_002073"
     CDS             155..1180
                     /gene="trpS"
                     /locus_tag="VXN30_002073"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1358614.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
BASE COUNT          409 a          187 c          267 g          395 t
ORIGIN      
        1 tattcaaatt tttatttgtc aattttgtgt aaattatatt tacagtatcc tttacacaaa
       61 tctgtaaaaa gcaaggctga tttattttga taaattacgg ttatttcatt aaaaaaatgc
      121 tataattgaa aggactatat cgaaggagaa caaaatgact aaacccatta ttttaacagg
      181 agaccgtcca acaggaaaat tgcatattgg acattatgtt ggaagtctca aaaatcgagt
      241 attattacag gaagaggata agtatgatat gtttgtgttc ttggctgacc aacaagcctt
      301 gacagatcat gccaaagatc ctcaaaccat tgtagagtct atcggaaatg tggctttgga
      361 ttatcttgca gttggattgg atccaaataa gtcaactatt tttattcaaa gccagattcc
      421 agagttggct gagttgtcta tgtattatat gaatctagtt tcgttagcac gtttggagcg
      481 aaatccaaca gtcaagacag agatttctca gaaaggattt ggagaaagca ttccgacagg
      541 attcttggtc tatccaatcg ctcaagcagc tgatatcaca gctttcaagg ctaattatgt
      601 tcctgttggg acagatcaga aaccaatgat tgagcaaact cgtgaaattg ttcgttcttt
      661 taacaatgca tataactgtg atgtcttggt agagccggaa ggtatttatc cagaaaatga
      721 gagagcaggg cgtttgcctg gtttagatgg aaatgctaaa atgtctaaat cactaaataa
      781 tggtatttat ttagctgatg atgcggatac tttgcgtaaa aaagtaatga gtatgtatac
      841 agatccagat catatccgcg ttgaggatcc aggtaagatt gagggaaata tggttttcca
      901 ttatctagat gtttttggtc gtccagaaga tgctcaagaa attgctgata tgaaagaacg
      961 ttatcaacga ggtggtcttg gtgatgtgaa gaccaagcgt tatctacttg aaatattaga
     1021 acgtgaactg ggtcctattc gtgagcgccg tattgaattt gctaaggata tgggagaagt
     1081 ttataatatg attcaaaaag gtagtgaaag agcgcgtgaa gttgcgggtc aaaccctatc
     1141 tgaggtaaaa ggggcaatgg gacttcatta ctttaactaa gtttatttta caagaaacta
     1201 tcttttctat ctcgaagaaa agatagtttt ttatttaccc ctgtaacatt tgacaaat
//
LOCUS       DAVLSL010000236         1226 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVLSL010000236 DAVLSL010000000
VERSION     DAVLSL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.238"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            7..573
                     /locus_tag="VXN30_002074"
     CDS             7..573
                     /locus_tag="VXN30_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001828886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358615.1"
                     /translation="MSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKESFLLIK
                     EKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKGNLFFPP
                     LGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALSHSTFLA
                     ETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            577..774
                     /locus_tag="VXN30_002075"
     CDS             577..774
                     /locus_tag="VXN30_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358616.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            764..>1226
                     /locus_tag="VXN30_002076"
     CDS             764..>1226
                     /locus_tag="VXN30_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358617.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
BASE COUNT          401 a          176 c          241 g          408 t
ORIGIN      
        1 aagataatgt caatttttca atccttttat gttgatgtca gtattgagga actgactttg
       61 actttaccaa tcagttttgt aaaaaggttt gagtatactc aaatgacttt tcataaggaa
      121 tcatttttat tgattaaaga aaagagaagg gggagtttga gttcatttgt tactcaggct
      181 cgcactatgg gtgaaaaagc caatatggat gttgttttgg tgttttcgaa gttatcagac
      241 agtgaaaaaa agcaattact tcaagctaga gttccgtttg tagactttaa gggaaacctc
      301 ttcttccctc cattgggact agtactcaat gcgaatgata ctgaaatccc taaggaatta
      361 acacctagcg aacaattaac gtggattgcc tttttattgc cagatggcga tataaagcag
      421 ataaaatctg tttctaacct tctatatggt ggtgcttatg ctttgtcgca ttcaactttt
      481 ttagctgaaa cggatgaaaa tactagctat gtcatatggc agagaaaatt caatcagtta
      541 tccttgccac tttctcagca tgttttaaaa tgaaagatgc tagagatatg gaaatatcgt
      601 ccttttgtat ctgagttttg gaatgatttt aaaaataatc atgataaaca atttgtagat
      661 ccgatttctc tttatttgac cttaaaagat gatgatgacc cacgtataga ggaagagagt
      721 gaagcactag aaaatatgat attacagtat ctgggagaag atgatgccag ctaatacgaa
      781 agttattttt caagaaatgt ttgcggattt tcagaactat tatgttctga ttgggggaac
      841 tgctacctct atcgtattgg attcgcaagg atttaaaagt cgcacaacaa aagattatga
      901 tatggtcatc atttatgaag taaaaaataa ggaattttat actaccttga atcatttttt
      961 agaattggga gagtatcaag gaagtcagaa agatgagaaa gcgcagcttt ttcgatttac
     1021 aacaactaat cctgagtttc cttctatgat tgaactattt agtatcttac cagaatatcc
     1081 attaaagaag gacggtcgag aaattccctt acattttgac caagatgcta gtttatcagc
     1141 cttattattg gatgaagatt attataatat attggtgcat gaaaaagaaa ccattcaggg
     1201 gtattcggta ttgagtaatt gtggtt
//
LOCUS       DAVLSL010000237         1222 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVLSL010000237 DAVLSL010000000
VERSION     DAVLSL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.239"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            145..988
                     /locus_tag="VXN30_002077"
                     /pseudo
     CDS             145..988
                     /locus_tag="VXN30_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT          382 a          203 c          309 g          328 t
ORIGIN      
        1 aaagagcaaa ctaggaaact agctgcaggt tgctcaaaac agtgttttga ggttgtggat
       61 agaactgacg aagtcagtaa caatacctac ggcaaggcga cgttgatgtg gtttgaagag
      121 agtataaaat agaaggagaa gcatatgaat acctatcaat taaataatgg agtagaaatt
      181 ccagtattgg gatttggaac ttttaaggct aaggatggag aagaagccta tcgtgcagtg
      241 ttagaagcct tgaaggctgg ttatcgtcat attgatacgg cggcgattta tcagaatgaa
      301 gaaagtgttg gtcaagcaat caaagatagc ggagttccac gtgaagaaat gttcgtaact
      361 accaagcttt ggaatagtca gcaaacctat gagcaaactc gtcaagcttt ggaaaaatct
      421 atagaaaaac tgggcttgga ttatttggat ttgtatttga ttcattggcc gaacccaaaa
      481 ccgctcagag aaaatgacgc atggaaaact cgcaatgcgg aagtttggag agcgatggaa
      541 gaacctctat caagaaggga aaatccgtgc tatcggcgtt agcaattttc ttccccatca
      601 tttggatgcc ttgcttgaaa ctgcaactat cgttcctgcg gtcaatcaag ttcgcttggc
      661 gccaggtgtg tatcaagagg aagtcgtagc ttactgtcgt gaaaagggaa ttttattgga
      721 agcttggggg ccttttggac aaggagaact gtttgatagc aagcaagtcc aagaaatagc
      781 agcaaatcac ggaaagtctg ttgctcagat agccttggcc tggagcttgg cagaaggatt
      841 tttaccactt ccaaaatctg tcacaacctc tcgtattcaa gctaatcttg attgctttgg
      901 aattgaactg agtcatgagg agagagaaac cttaaaaacg attgctgttc aatcgggtgc
      961 tccacgagtt gatgatgtgg atttctagaa aatcataaaa agaattgtac attattctaa
     1021 tttttgatat aatagtcagc aggaaagaaa gtcttatggc gttcttcaag cgagcttggg
     1081 acagtgggag ccaagtaggg caaaataatg agctggcgct ttctgtagta ttttcaaaaa
     1141 caatgaagta ataaattagg gtggaaccgc gtttctgacg cccctagtcg caaggcttgg
     1201 gtgttgaaag gtggttcttt tt
//
LOCUS       DAVLSL010000238         1198 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVLSL010000238 DAVLSL010000000
VERSION     DAVLSL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.240"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..1065
                     /locus_tag="VXN30_002078"
     CDS             <1..1065
                     /locus_tag="VXN30_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEV1358618.1"
                     /translation="STSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNKNFRQAINF
                     AFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYGKEWQGINF
                     ADGQDPYYNPEKSKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGVSSIKQSIE
                     SVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPSTYLDIFNT
                     NSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYADIQAWLIDS
                     SLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDEYAKAREKW
                     LKEKEESNKKAQEELAKHVK"
BASE COUNT          420 a          192 c          249 g          337 t
ORIGIN      
        1 tcaacttcat atttctttaa ttttaaccta gataggaagt cttacaatta tacttctaaa
       61 acaagtgaca ttgaaaagaa atcgactcag gaagcagttc tcaataaaaa cttccgtcag
      121 gctatcaatt ttgcttttga cagaacatct tatggggctc agtctgaagg gaaagaaggt
      181 gcaacaaaga ttttgcgtaa cctagtggtt cctccaaact ttgtcagtat caagggaaaa
      241 gactttggtg aagttgtagc ctctaagatg gtcaactatg gtaaggaatg gcaaggtatc
      301 aactttgcgg atggtcaaga cccttactac aatcctgaga aatccaaggc taagtttgcg
      361 gaagctaaga aagaactcga agcaaagggt gttcaattcc caatccactt ggataagact
      421 gtggaagtaa cagataaagt aggcatacaa ggagttagtt ctatcaaaca atcaattgaa
      481 tctgttttag gttctgataa tgtagtgatt gacattcagc aattaacatc agatgagttt
      541 gacagttcag gctactttgc tcaaacagct gctcagaaag actatgattt atatcatggc
      601 ggttggggac ctgattatca agacccgtca acctatctcg atatttttaa tactaatagt
      661 ggaggagttc tacaaaatct tggactagag cctggagaag ccaatgacaa ggctaaggaa
      721 gttggactgg atgtctatac tcaaatgttg gaagaagcta ataaagagca agatccagcc
      781 aaacgttatg agaaatatgc tgatattcaa gcttggttga ttgatagttc tttagttctt
      841 ccaagtgttt cgcgtggggg aacaccatca ttgagaagaa ccgtaccatt tgctgctgcc
      901 tatggtttaa ccggtacaaa agggattgaa tcatataaat acctcaaagt acaagataag
      961 attgtcacaa cagacgaata tgcaaaagct agagaaaaat ggttgaaaga aaaagaagaa
     1021 tccaataaaa aagcccaaga agaattggca aaacatgtca aataaaatct aattgtagat
     1081 aagtttgtgt aagaagtctt tgtgcttaaa aacgcataat atcaggtata taaaatccta
     1141 gatgttatgc gttttgttat aggaagattt attagatttt atcctcaaat tgagttat
//
LOCUS       DAVLSL010000239         1198 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVLSL010000239 DAVLSL010000000
VERSION     DAVLSL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.241"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            47..397
                     /locus_tag="VXN30_002079"
     CDS             47..397
                     /locus_tag="VXN30_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1358619.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDILAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            612..728
                     /locus_tag="VXN30_002080"
     CDS             612..728
                     /locus_tag="VXN30_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1358620.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            739..929
                     /locus_tag="VXN30_002081"
                     /pseudo
     CDS             739..929
                     /locus_tag="VXN30_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1056..>1198
                     /locus_tag="VXN30_002082"
     CDS             1056..>1198
                     /locus_tag="VXN30_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1358621.1"
                     /translation="MEQLHFITKLLDIKDPNVQILNIINKDTHKEIIAKLDYDAPSCP
                     ECG"
BASE COUNT          397 a          241 c          246 g          314 t
ORIGIN      
        1 ttattcacat cttatttcaa ctcactatag aaggaggaat aggaggattc tcagacatcc
       61 gggcatcagc ccaactaatg atttgattgc taagaaaata ttcagcaatc cagaaatcac
      121 ttgtcaattt attcgcgata tgctggactt gccagcaaaa aatgtgacca ttttggaggg
      181 aagcgatatt cacgtattac tctccatgcc ttactcggtg caggattttt ataccagtat
      241 agacatcttg gcggagttgg ataacggtac tcaagtaatt attgagattc aagtccatca
      301 tcagaatttt ttcatcaatc acttgtgggc ttacctgtgc agtcaggtta atcaaaatct
      361 tgaaaaaatt cgtcagcgag aaggtgatac tcactagatc tacaaacaca tcgctcctgt
      421 ttacgtcatt gctatcgtgg atagtaatta tttctcagat gacctggctt ttcatagctt
      481 tagtatgcgc gaagacacaa caggtgaggt attggcgatt accaacaatg gacaggaaaa
      541 ccatctggtt aagatggcat tcttggaatt aaaaaataca gagaaaccag caaagacaag
      601 gttcgcaagc catggttgga gtttttcggc aacaagccct ttacccagca accgcaacga
      661 gccattaccc aagcaaatca actgctggac tacaagagct ggtccgagga ggacaggaaa
      721 atgtttagtc aactacatat gcgagaagaa caagtcttgt tagcacagga ctatgccttg
      781 aacaaggact agagcgtggg aaagttgaag gaagggaaga aaggaaactt tttgccttcc
      841 tagacatagt acgccaaggt cttctgactt ctgaggttgc cagccagcaa ttaggtatgt
      901 cagtatctga atttgaggca ctgttgtaaa atggctccat aatatccata gtgggtaaat
      961 cccctatgga tattatggag cctattttgt gtagaaaaaa agtcccatat gacctataat
     1021 gaaaagcgac aaaacaactc attagaaaga atcatatgga acaattacat tttatcacaa
     1081 aattactaga cattaaagac cctaatgtcc agattttaaa catcatcaat aaggatacac
     1141 acaaggaaat catcgccaaa ctggactacg acgccccatc ttgccctgag tgcggaaa
//
LOCUS       DAVLSL010000240         1102 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVLSL010000240 DAVLSL010000000
VERSION     DAVLSL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.242"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..991
                     /locus_tag="VXN30_002083"
     CDS             <1..991
                     /locus_tag="VXN30_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1358622.1"
                     /translation="MCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPE
                     RVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLD
                     GLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKA
                     LGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDP
                     RANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKD
                     PLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            981..>1102
                     /locus_tag="VXN30_002084"
     CDS             981..>1102
                     /locus_tag="VXN30_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1358623.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLA"
BASE COUNT          430 a          155 c          186 g          331 t
ORIGIN      
        1 tatgtgtata gtttttagcg cttgttcttc taattctgtt aaaaatgaag aaaatacttc
       61 taaagagcat gcgcctgata aaatagtttt agatcatgct ttcggtcaaa ctatattaga
      121 taaaaaacct gaaagagttg caactattgc ttggggaaat catgatgtag cattagcttt
      181 aggaatagtt cctgttggat tttcaaaagc aaattacggt gtaagtgctg ataaaggagt
      241 tttaccatgg acagaagaaa aaatcaaaga actaaatggt aaagctaacc tatttgacga
      301 tttggatgga cttaactttg aagcaatatc aaattctaaa ccagatgtta tcttagcagg
      361 ttattctggt ataactaaag aagattatga cactctatca aaaattgctc ctgtagcagc
      421 atacaaatct aaaccttggc aaactttatg gagagatatg attaaaattg attcaaaagc
      481 cttaggtatg gaaaaagaag gtgatgagtt aatcaaaaat actgaagctc gtatatccaa
      541 agaattagaa aaacatccag aaatcaaagg aaaaatcaaa ggaaaaaaag tattatttac
      601 tatgattaat gctgcagata catcaaaatt ctggatttat actagcaaag atccaagagc
      661 aaattattta acagatttag gtctagtttt ccctgaatca ttaaaagaat ttgagagtga
      721 agatagtttt gcaaaggaaa tttctgcaga agaagcaaat aagataaatg atgctgatgt
      781 aatcataact tatggtgatg ataaaactct tgaagcttta caaaaagatc ctcttttagg
      841 taaaataaat gcaattaaaa atggtgccgt tgctgtaatt ccagataata caccgttagc
      901 agcctcatgc actccaacac cactttcaat aaactatact attgaagaat acctaaatct
      961 tttaggaaat gcatgcaaaa atgcgaaata aaaaacaaat aaacctaggc ataatttttg
     1021 taatctgcct aggtcttctt attacaatat ttttgtcatt aaagcttgga acaaaagaaa
     1081 ttaatatcag agatttttta gc
//
LOCUS       DAVLSL010000241         1101 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVLSL010000241 DAVLSL010000000
VERSION     DAVLSL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.243"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            29..>1101
                     /locus_tag="VXN30_002085"
     CDS             29..>1101
                     /locus_tag="VXN30_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV1358624.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVAKQSQAER
                     DYDAAMKKSEAAKKEYEEAKKDLEEAKAAQKKYEDDQKKTEEKAKLVQKADEERQKAN
                     VAVQKAYLKLREAQEQLNKSPNNKKNAAQQKLKDALAHIDEVTLKQKEAEANFNKEQA
                     KVVPEADKLAETKKKAEQAEKKEPELAKKVAEAKAKAEEAEKKAVEAKQKVDAEKYAL
                     EAKIAELEYEVQGLEKELKEIDESDSEDYIKEGLRAPLQSKLDAKKAKLSKLEELSDK
                     IDELDAEIAKLEKDVEDFKNSDGEQAEQYLVAAKKDLDAKKAELENTEADLKKAVDEP
                     ETPAPAPAPKPAPAPAPTPEAPAP"
BASE COUNT          460 a          184 c          240 g          217 t
ORIGIN      
        1 atgactatca gaaaagaggt aaatttagat gaataagaaa aaaatgattt taacaagtct
       61 agccagcgtc gctatcttag gggctggttt tgttgcgtct cagcctactg ttgtaagagc
      121 agaagaagct cccgtagcta agcagtctca agctgagaga gactatgatg cagcaatgaa
      181 aaaatctgaa gctgctaaga aagaatacga agaagctaag aaagatttag aagaagcaaa
      241 agctgctcag aaaaaatatg aagacgatca aaagaaaact gaagagaaag caaagcttgt
      301 tcaaaaagcg gatgaagagc gtcaaaaagc gaatgtggca gttcaaaagg cgtatttaaa
      361 actccgagaa gcacaagaac aattgaacaa aagtccaaac aataagaaaa atgccgctca
      421 acagaaatta aaagatgcat tagctcatat tgatgaggta acattaaaac aaaaagaggc
      481 ggaagcgaat ttcaataagg aacaagcaaa agtagttcct gaagcagata agttagctga
      541 gactaagaaa aaagcagaac aggctgaaaa aaaagaacca gaacttgcta aaaaagtagc
      601 agaagctaaa gcaaaagcag aagaagctga gaaaaaagct gttgaagcca aacaaaaagt
      661 ggatgctgaa aaatatgctc ttgaagctaa aatcgctgag ttggaatatg aagttcaggg
      721 actagaaaaa gagctcaaag agattgatga gtctgactca gaagattata ttaaagaagg
      781 tctccgtgct cctcttcaat ctaaattgga tgccaaaaaa gctaaactat caaaacttga
      841 agagttgagt gataagattg atgagttaga cgctgaaatt gcaaaacttg aaaaagatgt
      901 agaagatttc aaaaactcag acggtgagca agctgaacaa tatcttgttg cagctaaaaa
      961 agacttagat gctaaaaaag ctgaattaga aaatactgaa gctgacctta agaaagcagt
     1021 tgatgagcca gaaactccag ctccggctcc ggctcctaaa ccagccccag ctccagctcc
     1081 aactccagaa gccccagctc c
//
LOCUS       DAVLSL010000242         1034 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVLSL010000242 DAVLSL010000000
VERSION     DAVLSL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.244"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..988)
                     /locus_tag="VXN30_002086"
     CDS             complement(<1..988)
                     /locus_tag="VXN30_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358625.1"
                     /translation="MVAGQLLLIYAATINALVLNELIAMNLERFLKLSIYQMIVWCGI
                     IFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLN
                     DQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKIFASKMREVSL
                     NLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAG
                     AISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGELGGQLSSIIG
                     TKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEILKNLN"
BASE COUNT          365 a          178 c          132 g          359 t
ORIGIN      
        1 aatttaagtt ttttaatatt tctttatctc catacttata gcaaatattt tttgcttcat
       61 ataacggaaa atctctattc acctcatttg gttcgatatc attcatttta tttgactcaa
      121 ttggattaat tgaatacaat tttaaaaaaa taggcttcgt accaataata gaggataatt
      181 gacctcctaa ttcacctagc gctgtaaaaa taacacctgt tagtgctcct attgcttcaa
      241 tagtaccaat tttcactatt ccttttattg caagatagcc tgttaaaaaa acgagagata
      301 tctgaaaaaa aatattgaga aagaagctaa tagcgcctgc taacgtttct acagttgtct
      361 ttctttgtat aaccatcttt aataaaattc ctgcttcttt aattttctta ggcaatacat
      421 ataaaagatt caaggacgct aacacatcaa atccattcaa tatagtctca ctagatttta
      481 aaaaagcttc attttggcta gttaaattta gactaacttc tcgcattttc gatgcaaaga
      541 tttttggtac aagtagcata atcattaatg aaaacaaggt ggctacagtc aatgaccaat
      601 gatagtgatt aagagtcaca actgcaaata tagtaccaga aattcctttt attactaaaa
      661 aaagttgttt aaaagcctga tcatttaaag tctgaacatc attatttagc cacgaaagat
      721 atgttcctga tgatttacta tgaaattctt gataggtaga gttagagatg tctgtggcaa
      781 ctctatttcg aatctctaga ttaaactctt ggatcacttc aacctgataa tttttcacta
      841 cccagtcaag gaatattatc ccacaccaga caatcatttg gtagattgac aatttcaaaa
      901 accgctctaa attcatcgca attaattcat tcaacaccag agcattaata gttgctgcat
      961 aaattagcaa taattgacca gcaacaataa atatcgttaa taaactaaat ttttttatat
     1021 ttgattttat aata
//
LOCUS       DAVLSL010000243         1032 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVLSL010000243 DAVLSL010000000
VERSION     DAVLSL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.245"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            11..>1032
                     /gene="pcpA"
                     /locus_tag="VXN30_002087"
     CDS             11..>1032
                     /gene="pcpA"
                     /locus_tag="VXN30_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1358626.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNL"
BASE COUNT          395 a          149 c          173 g          315 t
ORIGIN      
        1 ggaaatttaa atgaaaaaaa ctacaatatt atcattaact acagctgcgg ttattttagc
       61 agcatatgtc cctaatgaac caatcctagc agatactcct agttcggaag taatcaaaga
      121 gactaaagtt ggaagtatta ttcaacaaaa taatatcaaa tataaggttc taactgtaga
      181 aggtaacata ggaactgttc aagtgggtaa tggagttact cctgtagagt ttgaagctgg
      241 tcaagatgga aaaccattca cgattcctac aaaaatcaca gtaggtgata aagtatttac
      301 cgttactgaa gtagctagtc aagcttttag ttattatcca gatgaaacag gtagaattgt
      361 ctactatcct agctctatta ctatcccatc aagcataaaa aaaatacaaa aaaaaggctt
      421 ccatggaagt aaagctaaaa ctattatttt tgacaaaggc agtcagctgg agaaaattga
      481 agatagagct tttgattttt ctgaattaga agagattgaa ttgcctgcat ctctagaata
      541 tattggaaca agtgcatttt cttttagtca aaaattgaaa aagctaacct tttcctcaag
      601 ttcaaaatta gaattaatat cacatgaggc ttttgctaat ttatcaaatt tagagaaact
      661 aacattacca aaatcggtta aaacattagg aagtaatcta tttagactca ctactagctt
      721 aaaacatgtt gatgttgaag aaggaaatga atcgtttgcc tcagttgatg gtgttttgtt
      781 ttcaaaagat aaaactcaat taatttatta tccaagtcaa aaaaatgacg aaagttataa
      841 aacgcctaag gagacaaaag aacttgcatc atattcgttt aataaaaatt cttacttgaa
      901 aaaactcgaa ttgaatgaag gtttagaaaa aatcggtact tttgcatttg cggatgcgat
      961 taaacttgaa gaaattagct taccaaatag tttagaaact attgaacgtt tagcctttta
     1021 cggtaattta ga
//
LOCUS       DAVLSL010000244          977 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVLSL010000244 DAVLSL010000000
VERSION     DAVLSL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.246"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..750)
                     /locus_tag="VXN30_002088"
     CDS             complement(<1..750)
                     /locus_tag="VXN30_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358627.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFVQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEK"
BASE COUNT          369 a          169 c          126 g          313 t
ORIGIN      
        1 tttttcttta taaagcaaag gtaaaacttt ataaaaaata tctaagtcaa cctcactctg
       61 attctgaagc aagtaggagt caagcaaatt ttcaaaaata gctatcaata tgattggtgt
      121 aaggtttttg tattcacatg tcaagtattc tgagtatcgt gcagatgata tttcttttac
      181 ataacttttt gaaagcaaga ttttttcaaa ttcatcatct attattaatc ttctatttcc
      241 aatccaagcg gtattaccac ccaaataaga ttgtacaaat agcaatatgt aagaaaatat
      301 gaacgtaaat actgaaagag gtagaattct atgcgtaaaa aaatagacga taatggtagc
      361 caggagtaaa gatatctctc gtgacaatcg aattcttttc agcatctttt ttaacagtcc
      421 gtaaggttgt ccttggcaat attcttgaac taaattaatc ggaatgacat ctctcaccat
      481 ttctggaaag tagagtaagt ttataggttg aaatctccaa ctcccatcat aagtaaatgg
      541 atatagaacc aaatacttaa aagatttttt tcttataaac ttaattatca caatatgaat
      601 gactgttgca atgaagtata ttaaatataa aagtaaaacc gccatcaata tacttacaaa
      661 aatccccttg gactcaatca atggaaaata aaatattgca actagaagcg aataaagtcc
      721 cgctaataaa tatttctgat catctttcat tcaaaacacc atattattta ctaagaatac
      781 atcaatacaa tagagcaact ctgtaagtca atatttgata aagcaaagct ttaacccctt
      841 ccccagacgt tagcttttct tcaacccact acagttgaca aggagcccaa attattgata
      901 aaaactttag tcatcatcgt ctctataaaa atattttcta tacactcttg ataataccaa
      961 aattaatgcc aatatca
//
LOCUS       DAVLSL010000245          961 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVLSL010000245 DAVLSL010000000
VERSION     DAVLSL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.247"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            47..844
                     /locus_tag="VXN30_002089"
                     /pseudo
     CDS             47..844
                     /locus_tag="VXN30_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          318 a          195 c          154 g          294 t
ORIGIN      
        1 ttatataggt attatggttc tgattttcta gtataaaaaa attcaaacta gaattcttct
       61 tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg gagctcgctg agacttctat
      121 cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa aagattgctc aaaagttaat
      181 tgaaaagatt tctatgaccg acattgatca tcagttgtct atttcaactt caactgtcat
      241 tcgcaagatc aataattttc actttgagca tgatttttcg cgtcttcctg agattatgtc
      301 ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc gggagatgga gatgagcttt
      361 attgctcagg actttgataa actcaatatc ataactgttc ttgagagcag aacacaagcc
      421 atcatccgaa atcccatgaa tacaaggcta tcaagcgcta ctggaagctc attcaacaag
      481 atagtcagaa actgagtgat aaacgatttt atcgccctac ttttcgtatg cacttgacta
      541 ataaagaaat tctagataaa cttttgagct attccgaaga cttgaaacac cactataatc
      601 tctatcagct cttgcttttc cacctctgtt tcagactttg tttaaaacct tcctcaaaga
      661 taaagagaaa atcgtcaacg cccttcaatt accttattcc aacgccaaac tggaagccac
      721 caataatctc atcaaactta tcaagcacaa tgcctttggt tttaggaact ttgaaaactt
      781 caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg ctttcttcaa cccactacag
      841 ttgacaaaga accgaaaaat gtctcgcctc taatatagtc aattgaaaca agaacaagag
      901 aaaagagcct cgtaaaaggt attgcaactt ggtaatacct ttttgaggtt ctttttgata
      961 t
//
LOCUS       DAVLSL010000246          947 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVLSL010000246 DAVLSL010000000
VERSION     DAVLSL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.248"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>947
                     /locus_tag="VXN30_002090"
     CDS             <1..>947
                     /locus_tag="VXN30_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1358628.1"
                     /translation="QQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDGQE
                     SYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTATLA
                     KEANNNTHIDNVHADGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIG
                     GLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLNNV
                     QRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGTYSNK
                     NNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIKEKKATFV"
BASE COUNT          351 a          121 c          202 g          273 t
ORIGIN      
        1 cagcaaagtg aaactggggt atatacatca tttaaaaatt tagtagatgc aatgaatagc
       61 aatcctaatg gcacattccg tttaggagca actatggatg ctcgtgaagt tgaacttcca
      121 gatggtcaag aaagttatgt gaataatgta ttccatggaa tattagtagg aacaaataat
      181 gagaaatatt atgctattta taatttgaaa aaaccattat ttggggaatt aaatggtgct
      241 acagtagaaa aactttcact aaaagatgtt aatatttccg caaaggacga tactgcaaca
      301 cttgcgaaag aagcaaataa caatactcat attgataacg ttcatgctga tggagcgata
      361 gctggggaac gtagcatagg aggattagta tcacaagtta ataattctac tatttctaac
      421 agtagctata caggtagaat aactaataca tataaaacag ttgctagtta tcaaatcgga
      481 ggtttagtag gcaaactttc tggtccgagg ggattaatag ataagtcgtt cgcatcaatc
      541 gatttatctt caaatgcaac acaaggagac cagtctattg gtggaatagt tggtgcagta
      601 gaaaatagtg cgcttattag taatagttat gctgaaggaa atctaaataa tgttcaacgt
      661 tttgcaaacg ttggtggagt agttggtaat ctttgggatc cggttggagg attagaaaaa
      721 tccggtcgac tttctaatgt actaagcgat gttaatgtaa cgaatggtaa tgctattgca
      781 ggttataatt ttaatggtat aaaagcaaat ggcacataca gtaataaaaa taataaggtt
      841 gtgaacgtag tacaagaaga tgatgaaatc ttaactaagg attctactgt tcaaagagga
      901 gaagtattag aagatgcaca aataaaagag aaaaaagcta cttttgt
//
LOCUS       DAVLSL010000247          945 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVLSL010000247 DAVLSL010000000
VERSION     DAVLSL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.249"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            58..>945
                     /gene="pavB"
                     /locus_tag="VXN30_002091"
     CDS             58..>945
                     /gene="pavB"
                     /locus_tag="VXN30_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1358629.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLV"
BASE COUNT          319 a          180 c          200 g          246 t
ORIGIN      
        1 aatttcagga gattatacta gagtcatcaa ataaagaaag actctaagga gaatcctatg
       61 aaattcaatc caaatcaaag atatactcgt tggtctattc gccgtctcag tgtcggtgtt
      121 gcctcagttg ttgtggctag tggcttcttt gtcctagttg gtcagccaag ttctgtacgt
      181 gccgatgtgg tcaatccgac cccaggtcaa gtcttacctg aagagacatc gggaacgaaa
      241 gagggtgact tatcagaaaa accaggagac accgttctca ctcaagcgaa acctgagggc
      301 gttactggaa atacgaattc acttccgaca cctacagaaa gaactgaagt gagcgaggaa
      361 acaaactctt ctagtctgga tacacttttt gaaaaagatg aagaagctca aaaaaatcca
      421 gagctaacag atgtcttaaa agaaactgta gatacagctg atgtggatgg gacacaagca
      481 agtccagcag aaactactcc tgaacaagta aaaggtggag tgaaagaaaa tacaaaagac
      541 agcatcgatg ttcctgctgc ttatcttgaa aaagctgaag ggaaaggtcc tttcactgcc
      601 ggtgtaaacc aagtaattcc ttatgaacta ttcgctggtg atggtatgtt aactcgtcta
      661 ttactaaaag cttcggataa tgctccttgg tctgacaatg gtactgctaa aaatcctgct
      721 ttacctcctc ttgaaggatt aacaaaaggg aaatacttct atgaagtaga cttaaatggc
      781 aatactgttg gtaaacaagg tcaagcttta attgatcaac ttcgcgctaa tggtactcaa
      841 acttataaag ctactgttaa agtttacgga aataaagacg gtaaagctga cttgactaat
      901 ctagttgcta ctaaaaatgt agacatcaac atcaatggat tagtt
//
LOCUS       DAVLSL010000248          937 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVLSL010000248 DAVLSL010000000
VERSION     DAVLSL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.250"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..209
                     /locus_tag="VXN30_002092"
     CDS             <1..209
                     /locus_tag="VXN30_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1358630.1"
                     /translation="SCRWKYDLYDMPFGKERKSLQGEIPEEFSMSAVDMSMIDHIPDM
                     IENGVDNLKIEGRMEWSLFTTYQQ"
     gene            230..700
                     /locus_tag="VXN30_002093"
     CDS             230..700
                     /locus_tag="VXN30_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV1358631.1"
                     /translation="MDAYLESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQ
                     LFGARRKIPEYKFVAEVISYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDL
                     HDAKGNKIDRAPNPMELLTIKVPQPVQAGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT          302 a          160 c          206 g          269 t
ORIGIN      
        1 agtcatgccg ttggaaatac gacctttacg atatgccatt tgggaaagaa cgtaagagtt
       61 tgcagggtga gattccagaa gaattttcaa tgtcagccgt tgacatgtct atgattgacc
      121 acattccaga tatgattgaa aatggtgtgg acaatctaaa aatcgaagga cgtatggagt
      181 ggagtctatt cactacgtat caacagtaac caactgctac aaggcggctg tggatgccta
      241 tcttgaaagt cctgaaaagt ttgaagctat caaacaagac ttggtggacg agatgtggaa
      301 ggttgcccaa cgtgaactgg ctacaggatt ttactatggt ataccatctg aaaatgagca
      361 gttgtttggt gctcgtcgta aaattcctga gtacaagttt gtcgctgaag tgatttctta
      421 tgatgatgcg acacaaacag caaccattcg tcaacggaac gtcattaacg aaggggacca
      481 agttgagttt tatggtccag gtttccgtca ttttgaaacc tatattgaag atttgcatga
      541 tgccaaaggc aataaaatcg accgcgctcc aaatccaatg gaactattga ctattaaagt
      601 cccacaacct gttcaagctg gagacatggt tcgagctctt aaagaggggc ttatcaatct
      661 ttataaggaa gatggaacca gcgtcacagt tcgtgcttaa tgtagttgtt tagttttaaa
      721 aaattatgca aagctccata tacaacactt aaacgagatt aaagaatggc gaaatccctt
      781 gatgcgcaag agattagctg tcttttttat tttttaagtg ataaagtcgg agctcaggta
      841 tcaaagccta tcaaattaaa caaagaagtg atgtcttaga tattttgaaa aaaattaata
      901 agcagaaaac tctctattat tttgttgtag agagttt
//
LOCUS       DAVLSL010000249          936 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVLSL010000249 DAVLSL010000000
VERSION     DAVLSL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.251"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>936
                     /locus_tag="VXN30_002094"
     CDS             <1..>936
                     /locus_tag="VXN30_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1358632.1"
                     /translation="DAFRFGTDEKEDKDTYQATFETNHPAIKHFFGPAGNNVVHNSNG
                     AYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRNGLGPEFYAKGLLQ
                     APDHPNDPTVTINSILKYDQSEESTRLQVADPTQRFGSVDDLNKYMHNMFDVIYMLEI
                     LEGKAVAKLDTNQKYDLLRKIENEYKPDPDGNSVYATNVVRRLKPEELTKLTTFNSLI
                     EHDIITRRGYVDEATYKRNGYYTINLFSPIYSALSSKIGTPGDLMGRRIAFELLAAKG
                     YKDGMVPYISNQYEKEAKAQGKVITSYGKQIGLVT"
BASE COUNT          360 a          134 c          175 g          267 t
ORIGIN      
        1 tgatgcattt agatttggta cagatgaaaa agaagataaa gatacatatc aagcaacttt
       61 tgaaactaat catccagcta taaaacattt cttcggtcca gcgggaaata atgttgttca
      121 taattctaat ggagcatatg ctacagggga tgcattctat tatatggcat atcgcatgct
      181 tgataaagat ggtgcggtaa cttatacaca cgaaatgaca cataactcag atagagaaat
      241 ttatcttgga ggctatggaa gacgaaatgg acttggacca gagttttatg ctaaaggact
      301 gctacaagct cctgatcatc ctaatgatcc aacagtcact attaattcca tattaaaata
      361 tgatcaatca gaagaatcaa caaggcttca agtagcagat ccaactcaac gatttggaag
      421 tgttgatgat ctaaataagt atatgcataa tatgtttgat gtaatataca tgcttgaaat
      481 actagaaggt aaagctgtag ctaaattaga tacaaatcaa aaatatgatt tattaagaaa
      541 aatagaaaat gaatataaac ctgatcctga tggaaattct gtttatgcaa cgaatgttgt
      601 acgtagatta aaaccagaag aacttactaa attaactaca tttaacagtt tgatagaaca
      661 tgatattata acaagaagag gttatgtaga tgaagcaact tataagcgga atggctacta
      721 tacaattaat ctattctcac caatttattc agcattgagc agtaaaatag gaacaccagg
      781 agatttaatg ggacgacgta ttgcatttga gttattggca gccaaggggt acaaagacgg
      841 tatggttcca tatatttcta atcagtatga aaaagaagcg aaagctcaag gaaaagttat
      901 tacatcatat ggtaaacaaa taggtttagt tactga
//
LOCUS       DAVLSL010000250          924 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVLSL010000250 DAVLSL010000000
VERSION     DAVLSL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.252"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            81..878
                     /locus_tag="VXN30_002095"
     CDS             81..878
                     /locus_tag="VXN30_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1358633.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          245 a          157 c          201 g          321 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atgatttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taag
//
LOCUS       DAVLSL010000251          923 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVLSL010000251 DAVLSL010000000
VERSION     DAVLSL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.253"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            52..714
                     /locus_tag="VXN30_002096"
     CDS             52..714
                     /locus_tag="VXN30_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1358634.1"
                     /translation="MNEQLPDEMTLAKQFACSRMTIKKALDLLVSEGLIFRKRGQGTF
                     VLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRIIHFKLEFANEFLAEKLQVALQS
                     PVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDILQKSIYNYIEGKLGLHIASATKI
                     LRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTPFEYSISRHRYDLFEFNSFALRH
                     SS"
BASE COUNT          304 a          152 c          167 g          300 t
ORIGIN      
        1 ttatttattt acaatgagat tcgtgaaaag attctttgta ataaatatac catgaacgaa
       61 caattacctg atgaaatgac attagctaaa cagtttgcct gtagtcgaat gacgatcaaa
      121 aaagctttag acttgttagt ttctgagggc ttaattttta gaaaacgtgg gcagggaacc
      181 tttgttctct ctcgtggcag ctcaaaaaga aaattaatcg ttccagaaag agatatccgg
      241 ggactgacaa aaatatctga agatgctcat tctacaattg actcgaggat tattcacttc
      301 aaattagaat ttgcaaatga atttttagca gaaaaactac aggtcgcttt gcagagtcca
      361 gtttataata tttaccgcct gcgtattatt gacggtaaac cttatgttct ggaacaaact
      421 tatatgagta ccgatgttat tccaggtatt actgaagata ttttacaaaa atcgatttac
      481 aattacattg aaggaaagtt aggattgcat attgccagtg ctacaaaaat cttacgagct
      541 tcttctagtt cagaaaatga gcaacattac ttgcagctcc ttccaacgga accggtattt
      601 gaagtagaac aagtggctta tttggataac ggaactccgt ttgagtactc gattagtcgt
      661 catcgctatg atttatttga atttaattct tttgcattac gacattcctc ctaggagaaa
      721 atgtgaaaat gaagccaatc ttttacagac tctagtttaa gaaaaattta aaacagggca
      781 agaaggtccc atctatgctt aaatggtttc tcttttctaa ataagatggc tttaaaagag
      841 tgatcgtttt atccatcatg ttgaaaaata tcttcgtata gcttatagag taggtactga
      901 aattgttcac ctgatctact tct
//
LOCUS       DAVLSL010000252          909 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVLSL010000252 DAVLSL010000000
VERSION     DAVLSL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.254"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(389..508)
                     /locus_tag="VXN30_002097"
     CDS             complement(389..508)
                     /locus_tag="VXN30_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1358635.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(612..761)
                     /gene="blpO"
                     /locus_tag="VXN30_002098"
     CDS             complement(612..761)
                     /gene="blpO"
                     /locus_tag="VXN30_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1358636.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
BASE COUNT          335 a          177 c          111 g          286 t
ORIGIN      
        1 aatagattat accaaatact ctccctaagc ggataaagaa agatggacat gatcagatac
       61 agaacgaaaa taatcagtat ttttttcttc ataagttttc ctcctaaatg tgcgatttat
      121 cttagttgag caagaacatt tacactgcta gtatagcact tattttgacc ttggatcact
      181 caaatcataa atggtcatca aaacctcttg aattgtaaaa attaaaaaag caagcatgaa
      241 aaacatactt tcctctttat attgtattga taccaacttg tttgtagact tttcatcctg
      301 ctatcacata tcattttgac aggcgaaaca atattaaaga aactcccctg taaattaagc
      361 tagcaaatac aggggagaaa tttatttttt agagagtact atccgtatcc tttttggaag
      421 attttgaaaa tatttttcta attaagtcat ccatataagg accaaatata ccaactacta
      481 aaccaataat aaaactttta aaatccataa ttaccaccaa catgttgctg cataggctac
      541 acctccaagt atagctccac ctgcagcacc agttacacct attcctatag caaatggtcc
      601 caatagaaat gtcaaaccgt tgttgcacac ccatcaattg cgccatatgc aacccctgct
      661 gcacaactaa tttttcttcc ccaatcaata tctccacctt caacgcaagc aagcatttca
      721 ttatccataa cagaaaattg tgacatcatt tttgtattca tatagtatgt cacttttcag
      781 ttacggaaca agtttaatat aaaaattatc aaaaaaacat aggcaataaa gagaaaaatt
      841 aatttatcat agattagaaa taatatgaca aaacaattca atgatgttaa ttcaatagtc
      901 ttttgtttt
//
LOCUS       DAVLSL010000253          906 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVLSL010000253 DAVLSL010000000
VERSION     DAVLSL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.255"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            51..791
                     /locus_tag="VXN30_002099"
     CDS             51..791
                     /locus_tag="VXN30_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1358637.1"
                     /translation="MENINIKIPEEKISVIIGSNGCGKSTLIKTLSRLIKPLEGEVLL
                     DNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDLEII
                     NRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQIEL
                     LDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLVKDI
                     FNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          344 a          153 c          150 g          259 t
ORIGIN      
        1 aaggtctgtg gtcaaataat cttacctgcg gttatgatga aaaaataatc ttggaaaata
       61 taaatataaa aatacctgaa gaaaaaatat cagttattat tgggtcaaat ggttgtggga
      121 aatcaacact cattaaaacc ttgtctcgac ttataaagcc attagaggga gaagtattgc
      181 ttgataataa atcaattaat tcttataaag aaaaagattt agcaaaacac atagctatat
      241 tacctcaatc tccaataatc cctgaatcaa taacagtagc tgatcttgta agccgtggtc
      301 gtttccccta cagaaagcct tttaagagtc ttggaaaaga tgaccttgaa ataataaaca
      361 gatcaatggt taaggccaat gttgaagatc tagcaaataa cctagttgaa gaactttctg
      421 ggggtcaaag gcaaagagta tggatagctc tagccctagc ccaagataca agtatcctac
      481 ttttagatga gccaactact tacttggata tctcatatca aatagaacta ttagacctct
      541 tgactgatct aaaccaaaaa tataagacaa ccatttgcat gattttgcac gatataaatc
      601 taacagcaag atacgctgat tacctatttg caattaaaga aggtaaactt gttgcagagg
      661 gaaagcctga agatatacta aatgataaac tagttaaaga tatctttaat cttgaagcaa
      721 aaattatacg tgaccctatt tccaattcgc ctctaatgat tcctattggc aagcaccatg
      781 ttagctctta ataattgaat attggaattc atttaaaagt cgcaaatttt gaactgaccc
      841 tcaaaagtta ggccaaaaaa ctaactaaag gaggtcagtt ttttaatggc aaatacagca
      901 cagaat
//
LOCUS       DAVLSL010000254          891 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVLSL010000254 DAVLSL010000000
VERSION     DAVLSL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.256"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            24..>891
                     /locus_tag="VXN30_002100"
     CDS             24..>891
                     /locus_tag="VXN30_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HEV1358638.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYK"
BASE COUNT          280 a          157 c          191 g          263 t
ORIGIN      
        1 atttaaagca ggaggttctg gaaatgaaaa agtctaagag caaatatcta accttggcag
       61 gtcttgtcct gggtacagga gttttattga gcgcgtgtgg aaattctagc actgcatcaa
      121 aaacctataa ctatgtttat tcaagtgatc catctagttt gaactatcta gcagaaaacc
      181 gcgcagcaac atccgatatt gttgcaaatt tggtagacgg gttattagaa aatgaccaat
      241 atgggaatat tattccatca ttagcagagg attggactgt ttctcaggac ggtttgacct
      301 atacctacaa acttcgtaag gatgccaagt ggtttacttc tgagggagaa gaatatgcgc
      361 ctgtaactgc ccaggatttt gtgacaggtt tgcaatatgc agctgataaa aaatcagaag
      421 ctttgtatct agtgcaggac tctgttgctg gtttggatga ctatatcact ggtaaaacaa
      481 gcgacttttc aactgtcggt gtcaaggcac ttgatgacca aacggttcaa tatactttgg
      541 ttaaaccaga actttactgg aattcaaaaa cacttgcaac gatacttttt cctgttaatg
      601 cagatttcct gaaatcaaaa ggggatgatt ttgggaaggc ggatccatct agtattttgt
      661 acaatggacc tttcttgatg aaagcacttg tctcaaaatc tgctattgaa tataagaaaa
      721 accctaatta ctgggatgct aagaatgtct ttgtagacga tgtgaaattg acctactatg
      781 atggtagcga ccaagaatca ctggaacgta attttacagc tggtgcttat actacggctc
      841 gtctttttcc taacagctcc agctatgaag ggattaaaga aaaatacaaa a
//
LOCUS       DAVLSL010000255          881 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVLSL010000255 DAVLSL010000000
VERSION     DAVLSL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.257"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..853)
                     /locus_tag="VXN30_002101"
     CDS             complement(<1..853)
                     /locus_tag="VXN30_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1358639.1"
                     /translation="MIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQT
                     MLRNPLASPDIIGVTSSSSITAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKD
                     GFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLI
                     VSIIATILVLFFNKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPI
                     ACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSP
                     YLIYLLIK"
BASE COUNT          327 a          151 c          130 g          273 t
ORIGIN      
        1 tttttattag taagtatatt aagtatggtg aacctaacaa gccagttaca acacctactg
       61 gatatctagc tggtaaaata ttttgagaga atatgtctga taacaaaact agtaaaattc
      121 caaccaatcc agctaatatt gggcttcttt tcttgccaat atttaaggct atgggaccag
      181 ctaaaaaaga tatacaagct attggtcctg taattgaagt agaaaaagca gttaaagata
      241 cagcgcaaaa aattaaaaca agccttgaaa gctcgggatt tgctccaagt ccgattgcta
      301 tttcttcacc aagttcaata atttctagtc ttttattaaa aaataaaact aatatagtag
      361 caataatact tactattaga acaagaggta tgtcatctaa ctttgtaaaa gataaagagc
      421 cactgagcca tctcataact tcttgtaatt catatcttgc tactttcaac aataaaaatg
      481 aggtgcctgc tcttgtgaca gcttgaaaac caatacctaa tattatcagt cttgctgctg
      541 aaaaaccatc ttttttagct agtaaaaata atattaaaga tgatgttagt ccacaagtta
      601 ttgaaataat tccagtagtt aaactatttg tttttaatac caatatgcaa aagaccgctg
      661 taatagatga agaacttgtg acaccgatta tatcaggact tgcaagagga tttcttaaca
      721 tagtttgaaa gataaatcct gccaatccaa aagaccagcc agctataatt cctgctaata
      781 attttggtaa tctaatttcc ataatcgaaa aactagctcc aggaacagtt tcactattta
      841 agactttaat caaagttgaa aaagaataac tttcatctcc g
//
LOCUS       DAVLSL010000256          846 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVLSL010000256 DAVLSL010000000
VERSION     DAVLSL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.258"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..827)
                     /locus_tag="VXN30_002102"
     CDS             complement(<1..827)
                     /locus_tag="VXN30_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1358640.1"
                     /translation="MPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGASLS
                     VVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMAL
                     SSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNAL
                     AMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDI
                     RTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVNN"
BASE COUNT          301 a          165 c          128 g          252 t
ORIGIN      
        1 tagttattaa cccctctatt tttcatagtt acataaataa gtactggacc cccgattatt
       61 gcagtaatta tccctacttc aatttcacct ggtttaccta acatacggcc gattatatca
      121 catataagca agagctctgc acctataaaa gatgaagaaa tggtcattgt gcgtatatct
      181 ttgcttataa ataagccaca aaagtgagga actataagac ctacgaagcc aataggtcca
      241 ccaattgcag taatacttga acataaaagc acacttgcaa ttattgcaag tgatcttatc
      301 ctattaacat taactccaag accaacagcc atttcatcac ccatagctaa agcgtttaaa
      361 tctgatgaaa taaatatagc tatcaagtga cctaaaatta taaaaggtag tagtgtagat
      421 atagaagata atgtagctgc tccaaggcta cctatttgcc aaaatctaaa tttgtctaag
      481 acgttattat tcggtaaaat taaaaaactt acaaaactgc ttaaagccat actaacacaa
      541 gttcctgata aggcaagttt tataggggta aggcctgctt ttccgcttac agcaatcgcg
      601 tatacaaaaa ttgcacttac taagccacca atgattgcaa aacttatatg gcttatgctt
      661 gatgaaattc ctaaaaaaga aagaccaatt actacactaa gacttgctcc tgtgtttata
      721 ccgagtatac ctggatcagc tattgggttt ctagtaactg attgcatcaa tacaccgctt
      781 atggcaagac tagaacctgc taaaattgca aaaatagttc taggtattct tttatatata
      841 attgat
//
LOCUS       DAVLSL010000257          838 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVLSL010000257 DAVLSL010000000
VERSION     DAVLSL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.259"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..>838)
                     /locus_tag="VXN30_002103"
     CDS             complement(<1..>838)
                     /locus_tag="VXN30_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1358641.1"
                     /translation="LFYFYSVVTIFLTSFAKWDYTNLNTPEFLGFDKLFENYRYVFKE
                     YPFFTEALINSVRWAVIGVIIQVPLAVSVAITLSKKLKGWKISRNLYIVPSIISSAAM
                     GLIFLQIYNPNYGVVNQIIHLFNPSFKDSVLLTPGLNIVAMTGAYIFFAGASTIMILG
                     QIFAIPEEVQEAAILDNITGWRKEWYITIPMIKGTIKTVSIMAATSGFLLYNEVFFLT
                     NGAAGTKSISFVIRELAVASSRTQYARANTIGVIQILGGMLIIVCINILFRERKRLKG
                     GK"
BASE COUNT          311 a          152 c          116 g          259 t
ORIGIN      
        1 catttcccac ctttcagtct ttttctttct ctgaataaaa tattaataca aacgataatc
       61 aacattccac ctaagatttg tataactcca attgtatttg cacgagcata ctgagttcgt
      121 gagctagcca ctgctaattc tcgaataaca aaactgatac tttttgttcc tgcagcacca
      181 tttgtcaaaa agaatacttc gttatagagc aaaaatcctg aagttgctgc cataattgaa
      241 acagttttaa ttgtcccctt aatcatcgga atcgtaatat accactcttt cctccaacca
      301 gtaatattgt ctaaaatagc agcttcttga acttcttctg gaatagcaaa aatttgccca
      361 agaatcataa tggttgatgc tcctgcaaag aagatataag cgccagtcat agctactata
      421 tttaatcctg gagtcaacag tactgaatct ttaaacgatg gattaaatag atgaataatt
      481 tggttaacaa caccatagtt tggattatag atttgaagaa aaatcaagcc catagcagca
      541 ctagaaatga tacttggtac aatataaaga ttcctagata tcttccagcc ttttaatttc
      601 ttagataatg taattgcaac agatacagct aaaggaactt gaataataac accaattaca
      661 gcccaacgaa cagaattaat tagagcttct gtaaaaaatg ggtattcttt aaaaacatac
      721 ctataatttt caaataattt atcaaatcca agaaactcag gggtgtttaa atttgtataa
      781 tcccatttag caaatgaagt taaaaagatt gttactacag aataaaaata aaatagaa
//
LOCUS       DAVLSL010000258          838 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVLSL010000258 DAVLSL010000000
VERSION     DAVLSL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.260"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            9..725
                     /locus_tag="VXN30_002104"
     CDS             9..725
                     /locus_tag="VXN30_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1358642.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
BASE COUNT          289 a          177 c          136 g          236 t
ORIGIN      
        1 gtaaaaatat ggcaattccc aaatatcaat acataaaaga cgaattaaaa aataaaatca
       61 tctctgggca gtttagcagt ggagataaat tttacactga agcagaactc atttcaatgt
      121 acgatgtcag ctctatcaca gttgttcgcg ccttaaacga ccttgccaaa gatggctata
      181 ttgtccgcca acaaggaaaa ggaaccttcg tttcacgtgc acgcaaacac aagcttgtag
      241 agttttcaga tattgaactc ttcaatgcta aagatgataa ggtaactgtt ctttcaattg
      301 aacgcggaaa caagcttgtt tacttggaaa aattgggtct acgcggcgat caattctact
      361 acaagattga acgtatccgc gaatcaaacg gtgtcgttta tatctaccat acatcctaca
      421 tcccagaaca atatatcaac gcaaattatc caaaccttga atactatagt tctatctata
      481 atcgtttcaa gttagactac cacattcaca tgaatgatga acactttgaa gaaatcaacg
      541 aaatagtctt tccaactcca gaacatgcgg cttcagtcct cggagtcgat gaacaattcc
      601 ctactgtcct acaaactaaa atcaccaaac tcgaatcaac tggccaagtt ttagaataca
      661 gtgaaactta caaacgatct gattactaca agattaaatt catctcatgt gaccgtgatc
      721 actaagaaga aagcctgagc ctaatcgctc gggctttttc tatgcttatt ataccacacc
      781 aaaaaggtag cgcttaccta tttttggaag attctataat ttgtggaata ttttatag
//
LOCUS       DAVLSL010000259          835 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVLSL010000259 DAVLSL010000000
VERSION     DAVLSL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.261"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..341
                     /locus_tag="VXN30_002105"
     CDS             <1..341
                     /locus_tag="VXN30_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1358643.1"
                     /translation="SKKIDISPKGTLLMSFKLSIGKVAILDIPATHNEAIISIFPYAN
                     KENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIISKVDL
                     LFQKVSQLFE"
     gene            complement(289..834)
                     /locus_tag="VXN30_002106"
     CDS             complement(289..834)
                     /locus_tag="VXN30_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1358644.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          302 a          127 c          116 g          290 t
ORIGIN      
        1 aatcaaagaa aatagacatt tcacctaaag gaactttatt aatgagtttt aaattatcta
       61 ttggaaaagt tgccatattg gatattcctg ccactcataa tgaagccatt atatcgatat
      121 tcccctatgc taataaagaa aatattatca gagattactt aatgatattt ttgccactta
      181 tctctacttt aggtgattca aaagatgcta taaaagggaa aacattaaat agtaccagta
      241 tctccgaatt attaatccct atttctaatc atgaagagat gaaaagaatc atttccaaag
      301 ttgatttact ttttcaaaaa gtttctcaac tttttgagta ataagttcct gttcctcaaa
      361 aggagctaac ggaattaata gctcgcttag tgtagtttta ggaatattat ataaagcttg
      421 acctgatagt ttagttattg ctttcaattg tttataaaat aacggagagg acaagttaaa
      481 taatagaaat tttgaaataa tctctgaact ttcgaatggt gttaattgga aaataaatcc
      541 accagccaca acaccatcat agtctttatc gattcttgca aactttccaa tatgttctaa
      601 agaggttgat acaggtgtta ttagctgatt atgttttaaa taaacttgtt cagaggagat
      661 gaattgtgta tcaatgtagt aatcattatc caacagagaa aattctaaag gcttaatatt
      721 accaccacgt ataattctaa cacctttatt attaatgctt aaatcgccct tcttgtaaga
      781 aagacctgta ttcattgaaa aaatatcttt tatttttata acaacccaat tcata
//
LOCUS       DAVLSL010000260          832 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVLSL010000260 DAVLSL010000000
VERSION     DAVLSL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.262"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..425
                     /locus_tag="VXN30_002107"
     CDS             <1..425
                     /locus_tag="VXN30_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1358645.1"
                     /translation="KIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLITKDKKTITDV
                     LEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALDGEIGKEIIR
                     LLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            422..>832
                     /locus_tag="VXN30_002108"
     CDS             422..>832
                     /locus_tag="VXN30_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358646.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFD"
BASE COUNT          320 a          107 c          165 g          240 t
ORIGIN      
        1 ggaagataaa agaaagaact ttttttaaaa atactgttgg atatgtattt cagaattatt
       61 ctttaataga taacaaaaca gtgtatgata atctgagcct tatcactaaa gacaaaaaaa
      121 caataactga cgtacttgaa aaagtaggac tgtcaagtga ctatttacac caaaaaatat
      181 atgaattgtc tggagggcag gctcaacgtg tagctattgc cagaatgtta atgaaaccac
      241 gtaaaattat cttagcagat gaacctacag gagctttaga tggtgagatt ggaaaagaaa
      301 ttattcgttt actattgaat gaaacagctg aagataaata tgtcattata gcgacacatg
      361 atccagctgt ctataacgaa gttgatgtga taattgacat gaaagatata gggtataagg
      421 tatgaaaaag aaaatttata ttgctttgat ctttgtaact ggggtactcg ccattttctt
      481 ttttggaaaa caaatgatta caaaagaaaa tatcaataag ccaacggtag agttaacaat
      541 ctatacgctt tcttctagtg atacagaaaa atggaataaa gtgagacaag tagaaacgga
      601 agaagctata tacttcataa ctgtaaaaga agtatcatct tcagaagaag tattttcaaa
      661 catcattgca aatggagctg ctacggggtt cggagttcgt gaagaagaag tgaagaaatt
      721 caataatggt ttaggagaca ccatagagga ctctaagcat aataaactaa ttgaaataga
      781 attttttact ttttctgatg atggcgcggg atttgtggta gcaaactttg ac
//
LOCUS       DAVLSL010000261          829 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVLSL010000261 DAVLSL010000000
VERSION     DAVLSL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.263"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            110..637
                     /locus_tag="VXN30_002109"
     CDS             110..637
                     /locus_tag="VXN30_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEV1358647.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKGIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            764..>829
                     /locus_tag="VXN30_002110"
     CDS             764..>829
                     /locus_tag="VXN30_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEV1358648.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRI"
BASE COUNT          444 a          116 c          126 g          143 t
ORIGIN      
        1 ataaagtatt ccaccaccaa gaacaataaa ataaaaacaa gcaaagggaa gtatagaaaa
       61 attaaaacct atacttccct tttttaattc aaacaaagga gagaaaagca tgataaacga
      121 agaaataagc aaggaagcag gtcaagcagc acaaaccata atatcataca caataaaggc
      181 aacaaaagaa tcaatcaatt tagaaaaaga aataagaaaa aagatgaatg aaactttaga
      241 aaaagcaaat ggaaacttaa aaagtcttat gggcgatgaa atgaaaataa aagatctcta
      301 caagaaagga caactagaaa atataagcat agatcaaatc gacctcaaag acttaaaaaa
      361 agaactaaac aaacttggag taagtttctc agtaatgaaa aacaaagaaa gcaaaaacta
      421 tgaaatattc ttccaagcca aaggcataaa agtaatggaa tatgccttta agcaagtcat
      481 agccaaggaa aataaaaaag aaaaagaaag tatcctaaaa caaataaaga aatacaaaga
      541 cctatccaaa aacaaagata agacaaaaga aaaaggaaaa aggaaagtaa aagaaaaagt
      601 aaaaccaaac aaaaaagata tgaccagaga aatctaaagg gagtaacgaa aagttacacc
      661 cttaaataac cataataaca agacttccga aaaacagaag tccagaattc cgaaaatcgg
      721 aaatcaagaa tttcgattat cgaaaatcaa gaaaggagca gatatggcaa caaacaaaag
      781 atactattgg ataaaattaa aagaagaatt tttcacagac aaaaggata
//
LOCUS       DAVLSL010000262          785 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVLSL010000262 DAVLSL010000000
VERSION     DAVLSL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.264"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..785
                     /locus_tag="VXN30_002111"
                     /pseudo
     CDS             1..785
                     /locus_tag="VXN30_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          247 a          146 c          169 g          223 t
ORIGIN      
        1 ggcgaaatgt aaaaaaatat gaggagttcg aactcgactc tctccttcaa gaaacacgtg
       61 gtggccgtaa ccatgcatat atgacgattg aggaaaagaa agtctttctt gcccgccatt
      121 tgaaggctac agaggcagga gaatttgtta caattgatgc cttatttcag gcttataaaa
      181 aggagttagg tcgttcctac acacgtgata ccttctatca actgttgaag caccatggtt
      241 ggcgaaatat tatgccacgt ccagaacatc ctaggaaagc agacgctcaa accattgtcg
      301 cgtctaaaaa taaaatctca attcaagaag aaaagaaagc gctttaaaag cagtagacgt
      361 tttcgtaagg ttcgcttgat gtaccaagat gaggctggtt tcggtagaat cagtaaactg
      421 ggatcttgtt gggctccaat aggagtaggt ccacatgtcc atagtcacta tatacgagaa
      481 tttcactatt gttatggagc tgttgatgcc catacaggcg aatcattttt cttaatagct
      541 ggtggatgta atactgagtg gatgaacgcc tttttagaag agctttcaca agcttatcca
      601 gatgattatc ttttactcgt tatggacaat gctatatggc ataaatcaag taccttaaag
      661 attccgacta atattggttt tacctttatt cctccataca caccagagat gaacccattg
      721 aacaagtgtg gaaagagatt cgtaaacgtg gatttaagaa taaggccttt cgaactttgg
      781 aagat
//
LOCUS       DAVLSL010000263          778 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVLSL010000263 DAVLSL010000000
VERSION     DAVLSL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.265"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..767
                     /locus_tag="VXN30_002112"
                     /pseudo
     CDS             1..767
                     /locus_tag="VXN30_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          280 a           68 c          169 g          261 t
ORIGIN      
        1 gtgataacat gccatggatg ggttgaatat aacttaaaaa agaaattctt gacttatttt
       61 gatttttgga catattctat gggagatgct tttatttgtg tatctgaaac tatgaaaaaa
      121 agattggaga gtataataaa aaaataagaa gatagttgca ataaataacg gaattaatgt
      181 atcaaatagc gatttagatg tagtaggtgt tcaagacttt aaaaaagaat tttgtattcc
      241 aaataacaaa aaaatcattt gttatgttga aaggttggat ccagaaaaaa ggccggatag
      301 attccttgaa tttgcagaaa aattattttt agtcagagaa gatgtgattt ttattatggc
      361 tggaaatggt agtatgtggg ctgctttgaa agaaaaaatt tgccatttga aatgtagaga
      421 taatttcagg ttacttggtg aaatctatcc agctactata gtttaccaaa tatcggattt
      481 attgtatatt ccttctgata cagaaggaat acctatgtgt gttttagagt caatgtcaca
      541 aggaactccg gttttagcta gtaatgttgg cgggttaagt gaaattattg aacatagggt
      601 tgatggattt ttatttgaga aggaagatgt tgagggagtg tgtgcttgtg ctaatttttt
      661 actcaatgat tctgagtatt tgaaatatat aggtgagaat agtaaatcaa aaataagaaa
      721 acatgagagt atatgatgaa ttattagaga agagtagtca tggataggaa tatagatc
//
LOCUS       DAVLSL010000264          748 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVLSL010000264 DAVLSL010000000
VERSION     DAVLSL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.266"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            10..748
                     /locus_tag="VXN30_002113"
                     /pseudo
     CDS             10..748
                     /locus_tag="VXN30_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          263 a          138 c          156 g          191 t
ORIGIN      
        1 aatagttcta tgaatgatga agcaagtaaa caactaactg atgcacgatt taagcgtctt
       61 gttggtgttc aacgcacgac ttttgaagag atgttagctg tattaaaaac agcttatcaa
      121 cttaaacacg caaaaggtgg acgaaaacct aaattaagcc tagaagacct tcttatggcc
      181 actcttcaat atgtgcgaga atatcgaact tatgaagaaa ttgcggctga tcttggtatt
      241 cacgaaagca acttaatccg tcggagccaa tgggttgaag taactcttgt tcaaagtggt
      301 tttacgattt caagaactcc tctcagttct gaggacacgg taatgattga tgcgacggaa
      361 gtacaaatca atcgccctaa aaaaagaatt agcgaatgat tctggtaaaa agaaatgcca
      421 cgctatgaag gctcaagcga ttgtcacaag tcaagggaga attgtttctt tggatatcac
      481 tgtgaactat agtcatgata tgaagttgtt caaaatgagt tgcagaaata tcggacaagc
      541 tggtaaaatc ttggccgaca gtggttatca agggctcatg aagatatatc ctcaagcaca
      601 aactccacgt aaatccagca aactcaagca aactcaagca aactcaagcc gctaacagct
      661 gaagataaag cctgtaacca tgcgctatct aaggagagaa gcaaggttga gaacatcttt
      721 gccaaagtaa aaacgtttaa aatgtttt
//
LOCUS       DAVLSL010000265          721 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVLSL010000265 DAVLSL010000000
VERSION     DAVLSL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.267"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..361)
                     /locus_tag="VXN30_002114"
     CDS             complement(<1..361)
                     /locus_tag="VXN30_002114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358649.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLY"
BASE COUNT          306 a          113 c           90 g          212 t
ORIGIN      
        1 ggtataaaag atactgactc ataccttgat accaaataaa attcggtatt tcaataagag
       61 catgaggtaa tattcctttt attgtagcta ctattccaat catctgacca gatataccca
      121 attgtattcc taaagatagc aaatcaaaaa gttgcaataa gggcgaaatc ggaaaacaga
      181 atagatatag caaaaaattt gtagaatttg cctgtaaaac tgtcaatgta aattgtaatt
      241 ccagtttcgg aggtatagta gcaaaagtct cattttttaa tataaagctt aaaagtaaac
      301 ctagtaaaaa acagactata caaaataaca catagtaaat cagaaaacca ctttttctca
      361 tctaatgccc cctactaaaa ttacaaatat tatcagaaaa caatataaaa ccagaaaact
      421 ccatctcaat aaactaactg tacctttaat taaaaataga gctttcttat aatagacata
      481 ggctataaaa taattgaatg gaatcaataa caaattcaac aaaatacttt ttatatcatt
      541 actataactt gtcaccggaa tcaaacataa caacaatgaa aattgtaaga aagagattaa
      601 atgcgtcaac atcattagtt gtgcattctt aaaaaagaaa ttcattttag aatgattaag
      661 agcagataat tgaagtaaag ctagatctac cccaattgag gatttatgat tccctattat
      721 t
//
LOCUS       DAVLSL010000266          716 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVLSL010000266 DAVLSL010000000
VERSION     DAVLSL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.268"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            85..252
                     /locus_tag="VXN30_002115"
     CDS             85..252
                     /locus_tag="VXN30_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEV1358650.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(312..665)
                     /locus_tag="VXN30_002116"
     CDS             complement(312..665)
                     /locus_tag="VXN30_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1358651.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
BASE COUNT          239 a          115 c          125 g          237 t
ORIGIN      
        1 gttaaataag atgaaatatt ttttaataaa gatttgaatt aaagtgtaaa ggaggagata
       61 gttattataa actacaagtg gatattgtgt cctgtatgtg gaaataaaac acgattaaag
      121 ataagggaag atactgaatt aaaaaaattc cccctctatt gtccgaaatg cagacaagaa
      181 aatttaattg aaataaagca gttcaaagta actgtgatta cagagccaga cgcaaagacg
      241 cagagccgat aaaatgagat taatacaatc tcattttatc ggctctttcc gttatgtatg
      301 gattctttta attagtcttc gatgtttctt gcttcgttga taccgctggc taaagattcc
      361 attaaggata gttctttgtc tgtaaagcta tccatgtatt tctctatctg taatcgtcgg
      421 gtgcttttta ccaagttatt agcaggtaag aaaaattcat caacggaaac atgaagtaac
      481 gatacaaggt cataaagaac ttgtatgctg gggtgttgcc ctttattttc aatattagtt
      541 aagtaccgtg ggtcaatttc aatcaatgct cccacttgtt cacgagttaa acctcgtttc
      601 aatcgagctt ctttaatggc taaaccaaag gctctaaaat catatttatc ttctttttta
      661 cgcatagtag accacctcta tacattttat tgttcctact gaattaaaaa caggta
//
LOCUS       DAVLSL010000267          702 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVLSL010000267 DAVLSL010000000
VERSION     DAVLSL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.269"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            241..>702
                     /locus_tag="VXN30_002117"
     CDS             241..>702
                     /locus_tag="VXN30_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1358652.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIF"
BASE COUNT          263 a           87 c          133 g          219 t
ORIGIN      
        1 ggaggaagtc ctgaaaaatt tgaaatttat ggttataagt gtcacaatca gaagacttct
       61 actgaaattc tctctctgag aaatcctgct cagattaaac aaaaaataaa aatagagaat
      121 ctttcgatag agaattttac cagagtaatt ggagatttta ctattcaaga agatgagata
      181 gaattggcac cgtactctat cgtgatactg aagaagtaaa gaaaaaattg tgaggtagag
      241 atgacgattt atattaataa ggacgagacc gtttttcatt tggcaatgaa agatagtagt
      301 tatattttta gaattttaga aaatggggaa cttcaacatc tacattttgg gaaaaggatt
      361 catgtcaagg aaaattataa ccaattgatg gcttatgaaa aaagaggatt tgaagtatct
      421 ttttctgaag aatttgagga tattcaacag tctatgatac aaaatgaata ttcttcatat
      481 gggaaaggag attttcggca tccagccttt caagttcaag gaatgaatgg tagtaggata
      541 acgacactaa aatatcaagg ttttgaactt gaaaaaggga aaaatcgtct taactctcta
      601 ccttcaacat ttgatgatat tggtcagtgt gcggaaacat taacgattat tttaacagat
      661 tccatattag atttaactgt tagactaaat tacacaattt tt
//
LOCUS       DAVLSL010000268          671 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.270, whole genome shotgun sequence.
ACCESSION   DAVLSL010000268 DAVLSL010000000
VERSION     DAVLSL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.270"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            256..473
                     /locus_tag="VXN30_002118"
                     /pseudo
     CDS             256..473
                     /locus_tag="VXN30_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          289 a           76 c          115 g          191 t
ORIGIN      
        1 tattttgtga tgaaaattta atatgtaaat ctcagacgat agaaattaaa aactctatcg
       61 tcttttttat actcaaaatt aggaggtaaa aatggtaagg ataagaggtc ccacttaaaa
      121 caatttatgg caaaataagg acggaataac acaacaaatt ctctaaaaca aatcactaaa
      181 tcaatgtaag attgaatgaa atcaatattt atgctataat taaataaatt taatgaagaa
      241 aaaaagaggg atattatggc acttaactat aaaccattat ggatacagtt agcaaaaaaa
      301 gactaaagaa aacagatgta ataactatgg caggacttac aacaaatgtt atggcacaaa
      361 tgggaaagga taaaccaatt acatttaaga atttagaaag aatatgtaag gctttatctt
      421 gcactcctaa tgatattatt agttttgaag ataattttag tgacgaggaa tagaaaatga
      481 ctttaaggac agaagatcaa gttagggatt atgcaagaga agtataggct ttaatgaagt
      541 tgaagaaaac atcaatcaag gtactggtca aataactact tttaatcaat taggcttcaa
      601 gggatattca gataagccag atggttggta tttacctaaa aatatgaatg atgtagcaat
      661 aatccttgaa a
//
LOCUS       DAVLSL010000269          671 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.271, whole genome shotgun sequence.
ACCESSION   DAVLSL010000269 DAVLSL010000000
VERSION     DAVLSL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.271"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..587)
                     /locus_tag="VXN30_002119"
     CDS             complement(<1..587)
                     /locus_tag="VXN30_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1358653.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYEYLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPCVSDSSMVSYMGKTKELKMYQE"
BASE COUNT          228 a          133 c           81 g          229 t
ORIGIN      
        1 tcctcttgat acatttttaa ttctttagtt tttcccatat aactaaccat actactatca
       61 cttacacatg ggaagtcctc ataatatatt actttataac gcataaattc aagtgccctt
      121 ccaatactat tcacaaaaac atgatcaaca tggtcaccaa gtgaaagcgg acaatatacg
      181 acacatttgt cgtctaaatg cattaacagc tcttttatga tatcattctt taatgtgtcc
      241 tcatttttta attcactata gatatgacgg tatagaaaat tgccatttct atctttccta
      301 tagagatatt catagtacga taagtgtcta aaatcacatt gtagacgttc acaagctaac
      361 ctgtcttctt tcttcctttc ttcaatcgga tatttcccaa ggttacacaa cttatgaaat
      421 tgcttagcag agggctgtag ctgttggctc aaagggtaac cagaaaatat agtaataaca
      481 agtacaattt ctccttctga agttaatttt gaaatataat caccacagga aaaaattgcg
      541 tcatctaaat gtggagataa aaagatatac ttagtattgt tactcataac cattccctct
      601 acaatttatc taaaaactca ctaagtgtct gattaaattc cacatcatca aaaaaattca
      661 ccttattctt a
//
LOCUS       DAVLSL010000270          662 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.272, whole genome shotgun sequence.
ACCESSION   DAVLSL010000270 DAVLSL010000000
VERSION     DAVLSL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.272"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(40..288)
                     /gene="blpK"
                     /locus_tag="VXN30_002120"
     CDS             complement(40..288)
                     /gene="blpK"
                     /locus_tag="VXN30_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEV1358654.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(357..626)
                     /gene="blpJ"
                     /locus_tag="VXN30_002121"
     CDS             complement(357..626)
                     /gene="blpJ"
                     /locus_tag="VXN30_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV1358655.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
BASE COUNT          203 a          186 c           77 g          196 t
ORIGIN      
        1 aataaagaat atagccttca taaaatttag ctttcatttt tatgatgtag cggtataggc
       61 taaatatcca caaaccactg ctcctccaat tcctcctatt gcagcgcccc atggtcctag
      121 aattttccca tatttcactc cacccgctgc acaacctaaa gcagcaacta cagctgctcc
      181 tccaacacct gctttggcaa aatctcccca attgcatccg ccaccttcaa cgcaagcaag
      241 catttcagta tccataacag aaaattgtga catcattttt gtatccatga caaatactcc
      301 ttttttaaaa aactaaaata aatcagaata gaatcctcat aattttacta taagtcttac
      361 caacttagtc ccaatttatc accaaccata cctcctaagc atgttaatcc acccccaatt
      421 gcaccaatgt gtgctccaac aaatgcacca gcaagtccag ctactcctaa agtggccaaa
      481 cctgctccag ttccaccagt tataattccc gtagtgactc ctgtaatcag tgcattttga
      541 caatcagtgg agctataccc cccttcaact ttcgcaagca tttcagtatc cataacctct
      601 aactgtgaca acatttttgt attcatgatg aatacctcct ttttattttc aatttgttac
      661 ca
//
LOCUS       DAVLSL010000271          658 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.273, whole genome shotgun sequence.
ACCESSION   DAVLSL010000271 DAVLSL010000000
VERSION     DAVLSL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.273"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(1..658)
                     /locus_tag="VXN30_002122"
                     /pseudo
     CDS             complement(1..658)
                     /locus_tag="VXN30_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; internal stop; incomplete; missing N-terminus
                     and C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
BASE COUNT          216 a          120 c           83 g          239 t
ORIGIN      
        1 tctttggctt tcgttccaat atttgtttaa ctatgaactg ttttctattt gtgtttgaaa
       61 tatcaatcgc ttctatcact ggggcattta tttctataaa ttcttttttt aattgtttag
      121 tatctttggg aagacaatat cctccaaatc caaaagaagg attattataa aaatttccaa
      181 ttcttggatc taaacaaaca ccttttatta caacttcagc atttaaactt ctcctctcag
      241 caaaaaaatc tagttcatta aaaaaagcaa cacggagagc taagaatgtg ttagaaaaaa
      301 gcttaattgc ttctgcttca gtaggagaaa ctaacataac atttttaata ttggcaatac
      361 tatgagtact aatcgaaagg aacaactctg caatttttct tccttcaact gtctcatctc
      421 caacaactat gcgacttgga tataaattat catatataga acaaccttct ctcaaaaatt
      481 cagggacaaa aatgatattt tttgtatcaa acagcctttt taatttgttt gaaaagccga
      541 tcggaactgt tgactttcaa ataatctttc cattaggttt taccctcaga atcttcgata
      601 ccgtttgttc gatttcatat gtattaaaac taccaatttt ctcatcataa tctgtcgg
//
LOCUS       DAVLSL010000272          656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.274, whole genome shotgun sequence.
ACCESSION   DAVLSL010000272 DAVLSL010000000
VERSION     DAVLSL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.274"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            48..>656
                     /locus_tag="VXN30_002123"
     CDS             48..>656
                     /locus_tag="VXN30_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1358656.1"
                     /translation="MAEKNSNAQLARQFIIGLPKELSLSENKNLVERYIKENLTSQGM
                     IVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKEYILDEKGEKVLNKNGKP
                     KTRKVELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVDHRSFKRQNSDYLPTIHL
                     GSAASAMERKGIETDKGNYNREIRKYNQLVKTIKEEIKTLKGW"
BASE COUNT          320 a           78 c          120 g          138 t
ORIGIN      
        1 taccaaaaga atttaatgat aggtctacct tatggaataa agtagaaatg gcagaaaaaa
       61 attctaatgc acaactagca agacaattca taataggact accaaaagag ttatctttaa
      121 gtgaaaataa aaacctggtt gaaagatata taaaagaaaa tttaacatca caaggaatga
      181 tagtagatta tgcaattcat gatgagagtc aagataaaaa tggaaatatc cattgtcata
      241 taatgaccat aatgcgacca ataaacgaaa aaggagagtt cttagccaag tcaaaaaaag
      301 aatatatcct agatgaaaaa ggagaaaaag tattaaacaa aaatggaaaa ccaaagacaa
      361 gaaaagtaga actaacaacc tggaacgata caggcaatgt agaacaatgg agagaaaatt
      421 tttcagatct ttgtaacaaa tatttagaaa gagcaggagc agaaaaaaga gtagaccata
      481 ggagttttaa aagacaaaat tcagattatc taccgacaat ccatttagga tcagcagcaa
      541 gtgcaatgga aagaaaggga atagaaactg acaagggaaa ctataataga gaaataagaa
      601 aatataatca acttgtaaaa acaataaaag aagagataaa aacattaaag ggttgg
//
LOCUS       DAVLSL010000273          650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.275, whole genome shotgun sequence.
ACCESSION   DAVLSL010000273 DAVLSL010000000
VERSION     DAVLSL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.275"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            293..592
                     /locus_tag="VXN30_002124"
     CDS             293..592
                     /locus_tag="VXN30_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1358657.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          245 a          123 c          111 g          171 t
ORIGIN      
        1 aatagataga tgtaacatgt ttgcaaacaa agcatacgaa cctttagtaa aatcatttcc
       61 atgaaactag aatagagccc tcttagcaaa aatcattatt ttaatttatt tctaatcact
      121 ccttgacata aataactctc accaataaaa gactatgtct taaaaaaatg gtataataaa
      181 atcaatactt gggcttgatg gctatgctac taataacaat taggagagaa aatcaggcac
      241 ttgttaacaa caaggattat ccccttgaga tgaaaggaac tttagaaatc ttatgatgaa
      301 catgcaaaac atgatgcgtc aagcacaaaa acttcaaaaa caaatggaac aaagccaagc
      361 tgaacttgct gctatgcaat ttgttggcaa atctgctcaa gatcttgtcc aagcgacctt
      421 aactggcgat aagaaagttg tcagcattga tttcaatcca gctgtcgttg acccagagga
      481 ccttgagact ctttctgata tgaccgttca agccatcaac tctgctcttg aacaaatcga
      541 tgaaactacc aagaaaaaac tgggtgcttt cgctgggaaa ttacctttct aaaaacaagg
      601 agctagaaca atgcttgtcg ataacaaagg ctaagaaagg tgcaaaaatg
//
LOCUS       DAVLSL010000274          650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.276, whole genome shotgun sequence.
ACCESSION   DAVLSL010000274 DAVLSL010000000
VERSION     DAVLSL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.276"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            36..416
                     /locus_tag="VXN30_002125"
     CDS             36..416
                     /locus_tag="VXN30_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358658.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
BASE COUNT          199 a           94 c          136 g          221 t
ORIGIN      
        1 ttttatttga aaaaaataaa aaaggagatt ttattatgaa attaaaaaca cttgctttaa
       61 ttagtggtat cgtcggtctt gtgggaggaa ttttacttct tattggtcct tttgtcttgt
      121 tgggaacagc ggtaaacaca gttgctacaa ctcttaatgg aggagctact gcaggggctt
      181 tttcaggtgt tgccttactc ttgaatgcct tgaagattgc aaatcttgtt cttggtatca
      241 ttgctattgt ttactataaa ggagataatc gtgtaggtgc agctccgtct gtactaatga
      301 ttgtttttgg tggagttagt ctcattccat tcttaggatg ggttgggggg attcttgtta
      361 ttatcggagg atctctattc cttgcaacat tgaagaaatt caaatcagaa gaataaaagg
      421 tattttagca tgaaaagaac aaaaaagttt atcggtatag gagtagctct attatctctt
      481 tttcttctag ttgcatgtgg aacataaagt tcaaaggata cttcaacaag taatgatgag
      541 aagacagtag caacatccaa tagttcaaaa gaaacaatca ctttcgatac accggttgta
      601 acagacgatg tgattgaatc aatacgcact tatgcagatt atatagatct
//
LOCUS       DAVLSL010000275          631 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.277, whole genome shotgun sequence.
ACCESSION   DAVLSL010000275 DAVLSL010000000
VERSION     DAVLSL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.277"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..631
                     /locus_tag="VXN30_002126"
                     /pseudo
     CDS             1..631
                     /locus_tag="VXN30_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          214 a          118 c          115 g          184 t
ORIGIN      
        1 gaaaagttga tagagataga cttaaaaact atcttactga caatccagac gcttatttga
       61 ctgaaatagc ttctgaattt ggctgtcatc taactaccat ccactatgcg ctcaaagcta
      121 tgggttacac tcgaaaaaaa gaaccacacc tactatgaac aagacccaga aaaagtagcc
      181 ttatttctta agaattttaa tagtttaaag cacctagcac ctgtttagat tgacgaaaca
      241 ggattcgata cttattttta tcgagaatat ggtcgctcat taaaaggtca gttaataaga
      301 ggcaaagtat ctggaagaag atatcagaga atttctttgg ttgcaggtct aacaaatggt
      361 gagttaatcg ctccaatgac ttacgaagag acgatgacga gcgacttttt tgaagcttgg
      421 tttcagaagt ttctcttacc aacattaacc acaccatcgg ttattattat ggataatgca
      481 agattccata gaatgggtaa gttagaactc ttgtgtgaag agtttgggta taacttttta
      541 cctcttcctc cctactcacc tgagtacaat cctattgaga aaacatgggc tcatatcaaa
      601 aagcacctca aaaaggtatt accaagttgc a
//
LOCUS       DAVLSL010000276          630 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.278, whole genome shotgun sequence.
ACCESSION   DAVLSL010000276 DAVLSL010000000
VERSION     DAVLSL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.278"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..591)
                     /locus_tag="VXN30_002127"
     CDS             complement(<1..591)
                     /locus_tag="VXN30_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1358659.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQSNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISI"
BASE COUNT          217 a          111 c           76 g          226 t
ORIGIN      
        1 tatacttatc cattcatttg cctttgcttt aaagaaaaaa tcagcggcag gagcgtccat
       61 cttacaattt aatatttcca ttgccactct ttctaaggcc tttgtaagta ttatttgatt
      121 cgattgaagc aaggttgaat aaaaagattc tggattaatg ttaatagatg ctaaatgttt
      181 ttctattagc tcttttttaa aaccaatgga aacagcaaga taacaacaat tctcgtgtaa
      241 taaaaaaaca aaatcatctt ttatattatc aaaatcaaaa gtacataaag agtttgcggt
      301 aatagtttga tacggattaa acttttctcc gtttgcactg acaatgtaac ttgaataaat
      361 tgaaacatag tctgacatac tataagtgct attttgaact acttcctctt tgatataaaa
      421 atcatgtata tcgataatga agctgcctcc ttcataaaac caatataggc cttctgcata
      481 agttgaattc tcttttttcc aacaaaatgt atgacctgca cttgaatact tctcattgtt
      541 tttacattca tctactttca tataatcttt tacaaaatca taatatgaca taacacacta
      601 tcccttttag acaatattcc aattagcctt
//
LOCUS       DAVLSL010000277          619 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.279, whole genome shotgun sequence.
ACCESSION   DAVLSL010000277 DAVLSL010000000
VERSION     DAVLSL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.279"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..81
                     /locus_tag="VXN30_002128"
     CDS             <1..81
                     /locus_tag="VXN30_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickase"
                     /protein_id="HEV1358660.1"
                     /translation="GKLKKLLSDKEKLSPKKWNEEKILLM"
     gene            407..580
                     /locus_tag="VXN30_002129"
     CDS             407..580
                     /locus_tag="VXN30_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358661.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
BASE COUNT          318 a           69 c          100 g          132 t
ORIGIN      
        1 gggaaattaa aaaaactcct atcagataag gaaaaactaa gtcctaaaaa gtggaatgaa
       61 gaaaaaattc ttttaatgta aaatcttgaa gaaataaata aagaaaaaga caagataaaa
      121 gatgaatacc aggaaattaa tcatataaaa tattcagtag attttgtaaa caaagaactt
      181 ggtatagatt tatccataga aatagataag ctaataaaac agggagaaaa accaagtgta
      241 atagcacaga ttaaaaagtt ccaagaccaa gttaataaag ataatgaata cagagaaatg
      301 atgaaaaaca agaaaatgga tcaagaaaga taagacctgg taaaaatatc ttatccaagc
      361 tataatatga ccaaacaact aaagtcagat ctaggaggta atagagatga ataaaaggca
      421 agagctaata gatgaactca taaaagcaaa tgaagatgga acatacaaaa cctataaaag
      481 cacagaagaa ataaaagcaa tgaacaatga agaaatacag attatatatt ccaacatgaa
      541 aaactatcta tcagacaaaa gaacacacat aaactattaa aggtggaaag gtagtgtaag
      601 ctatttttaa aagtataaa
//
LOCUS       DAVLSL010000278          590 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.280, whole genome shotgun sequence.
ACCESSION   DAVLSL010000278 DAVLSL010000000
VERSION     DAVLSL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.280"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..590
                     /locus_tag="VXN30_002130"
                     /pseudo
     CDS             1..590
                     /locus_tag="VXN30_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          230 a           96 c          125 g          139 t
ORIGIN      
        1 tcgttacgga atagagttcg tcaaaaaagg gaaaaatcgt tactattctc ctgatttaaa
       61 acaagaaatg attcataaag tctgacatga aggctagact aaagatagag tttctcttga
      121 atacggtctc ccaagtcgta cgatacttct taactggcta gcacaataca ggaaaaacgg
      181 gtatactatt gttgagaaaa caagagggag agtacctaaa atgggacgta agccaagaac
      241 gagacctgaa gagaggacag aattagaacg tcttcaagca gaaaatgagt acctgagagc
      301 ggagaaggcc atcctaaaaa agttaagaga actccgattg aaggaggaaa aagagaaaga
      361 agaaagacag aaattgttca agaattaatg actgagtttt cgttagatat tcttctaaaa
      421 gccattaaac tagctcgttg gacctactac tatcacttga aacagctaga caaaacagat
      481 aaggaccaag agcttaaagc tgaaattcaa tccattttta tcgaacacaa gggaaattat
      541 acttatcgcc gggttcattt agaactaaga aatcgtggtt atctggtaaa
//
LOCUS       DAVLSL010000279          573 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.281, whole genome shotgun sequence.
ACCESSION   DAVLSL010000279 DAVLSL010000000
VERSION     DAVLSL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.281"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..259
                     /locus_tag="VXN30_002131"
     CDS             <1..259
                     /locus_tag="VXN30_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1358662.1"
                     /translation="NKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQF
                     GENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            334..531
                     /locus_tag="VXN30_002132"
     CDS             334..531
                     /locus_tag="VXN30_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1358663.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          203 a          101 c           76 g          193 t
ORIGIN      
        1 aaataaaact atcgttttaa gaatcccagt tattcctaat tttaacaata gtttagagga
       61 tgcagaaaaa ttcgctactc tatttaactc attaaatatc gaccaagttc aactactccc
      121 ttttcatcaa tttggtgaaa acaaatatcg tttattaaat cggaaatatg aaatggatgg
      181 agtcaacgca cttcatcctg aagatcttat tgattatcaa aaggtatttc tgaaccacca
      241 tattaattgt tatttctagt ttatttcctt gaaatgctct agctatttgc agataacaag
      301 catctatata aggttatcaa agaagacttc cccatgactc taaatgaata tatcttacaa
      361 tatcgcctga aacaagctat agataagatg gctgaatctc ccaactcctc tttaagcgat
      421 atctctgacc aagttggatt ttcagactat aaatattttg ccaaagtatt taaaaagtat
      481 ctccatattt ccccaaagaa attgaaatca ctcggaagaa tagtaaaata ggctattctt
      541 ctttttgtag agaattttac tctaatatag tga
//
LOCUS       DAVLSL010000280          555 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.282, whole genome shotgun sequence.
ACCESSION   DAVLSL010000280 DAVLSL010000000
VERSION     DAVLSL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.282"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(280..450)
                     /locus_tag="VXN30_002133"
     CDS             complement(280..450)
                     /locus_tag="VXN30_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358664.1"
                     /translation="MKKENEYVILTTALLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(463..555)
                     /locus_tag="VXN30_002134"
     CDS             complement(463..555)
                     /locus_tag="VXN30_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEV1358665.1"
                     /translation="MKGGKIVESGNHKYLMTLGGEYYSLYTKRK"
BASE COUNT          190 a          108 c           71 g          186 t
ORIGIN      
        1 atgaaaatca aagagcaaac taggaagcga gccgcaggct gtacttgagt acggcaaggc
       61 aaagctgacg tggtttgaag agattttcaa agagtattaa acaaaaagaa aaaccgctac
      121 tcctaagaat agcgatttaa tcatgttttt aagctactac tgtagttgct ctattattta
      181 agtgttatga ataacttcaa tatctttaaa gctttttgtt agatttataa tttcaactat
      241 agccatctct actccttagt ttacagcaaa aaattatgct taattcttat tgtttttgta
      301 aacaatcagc gaacccaata ctattccatt caacaagcct aaagaaatac catattcaac
      361 tggaaaatct aaaaaaatag caaacactat tccaatcatc acccctagta aggctgttgt
      421 taaaattaca tattcatttt ctttcttcat tttctttaca cctcatttcc tttttgtata
      481 taagctgtag tactctccac caagagtcat taagtactta tgatttcctg actcaacaat
      541 cttaccacct ttcat
//
LOCUS       DAVLSL010000281          551 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.283, whole genome shotgun sequence.
ACCESSION   DAVLSL010000281 DAVLSL010000000
VERSION     DAVLSL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.283"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>551
                     /locus_tag="VXN30_002135"
     CDS             <1..>551
                     /locus_tag="VXN30_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1358666.1"
                     /translation="PSCPECENQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYH
                     CSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFH
                     FKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLKGRTQAVIRNHFLRYDRA
                     VRCRVKIITMDMFSPYYDLARQL"
BASE COUNT          172 a          118 c           86 g          175 t
ORIGIN      
        1 ccatcttgcc ctgagtgcga aaaccaattg aagaaatatg actttcaaaa accttctaaa
       61 attccttatc ttgaaacgac tggtatgcct actagaattc tccttagaaa gcgtcgattc
      121 aagtgctatc actgttcaaa aatgatggtc gctgaaactt ctatcgtcaa gaagaatcac
      181 caaatccctc gtatcatcaa ccaaaagatt gctcaaaagt taattgaaaa gatttctatg
      241 actgatattg cccatcagct ttccatctca acttcaactg ttattcgtaa gctcaatgac
      301 tttcacttta aacatgattt ttcttgtctt cctgagatta tgtcttggga tgagtatgct
      361 tttacaaaag gaaagatgag cttcattgcg caagatttta acaatctcaa tatcatcact
      421 gttcttaagg gaagaacaca agctgtcatc cgaaatcact ttcttcgcta cgatagagcc
      481 gtccgatgtc gcgtcaaaat cattactatg gatatgttta gtccttacta tgacttagct
      541 agacaacttt t
//
LOCUS       DAVLSL010000282          543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.284, whole genome shotgun sequence.
ACCESSION   DAVLSL010000282 DAVLSL010000000
VERSION     DAVLSL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.284"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>543
                     /locus_tag="VXN30_002136"
     CDS             <1..>543
                     /locus_tag="VXN30_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HEV1358667.1"
                     /translation="SINAFCIGGEKGINEIPEVTKIAKFYGINLLVDTVMSQDLEYFP
                     DIVWRLEGSLFECGVILQYYLGRLLFQKGNTSIFCGESADEIMTFKYHSVNYNQFCND
                     KQKSVYFSYSDYPFYVTNSIVLKKNNLLLHSFGIHPRYPYKMSEIVEMSKKISNLNDK
                     KEFHKKNCELRFQDSVLDNI"
BASE COUNT          197 a           64 c           97 g          185 t
ORIGIN      
        1 cttcaattaa tgcattttgc ataggaggag aaaaaggaat taatgagatt cctgaagtaa
       61 ctaaaattgc aaaattttat ggtataaatt tattagttga tactgtaatg agccaagatt
      121 tagaatattt tcccgatata gtatggagat tggaaggaag tctctttgaa tgtggtgtca
      181 tactgcagta ctatttaggc aggttgttgt tccaaaaagg gaacacaagt attttttgtg
      241 gcgagtcagc ggatgaaata atgactttta agtatcattc tgtgaactac aatcaatttt
      301 gtaatgataa acagaagtca gtatatttta gttattccga ctatccattt tatgttacaa
      361 attcaattgt tttaaagaaa aataacctac ttttgcatag ttttggcatt catcctagat
      421 atccatataa aatgtctgag attgttgaaa tgtctaaaaa aatatcaaat ctaaacgata
      481 aaaaagaatt tcacaaaaag aactgtgagt tgaggtttca agatagtgtt cttgataaca
      541 taa
//
LOCUS       DAVLSL010000283          542 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.285, whole genome shotgun sequence.
ACCESSION   DAVLSL010000283 DAVLSL010000000
VERSION     DAVLSL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.285"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
BASE COUNT          206 a           93 c           71 g          172 t
ORIGIN      
        1 tatcgacaag ttatcctaaa agtgttatta tgttgttgtg ttatagattg attgaatcta
       61 cctaaagaat cctattcaat tactagaact atcacatact caaggttagc tcacaaatga
      121 gcaactattt tggttacaat atctactaaa tttaagtcaa acaaataatt tagtcaaaat
      181 taaaaaaata gaggaacata aatatgatta caaaacagaa tgtaacagtg ttctacgatt
      241 tttactagat aaaactgtaa attctgaagg aaggatcact tcttcaacag aatttggaaa
      301 tttcgtaagt aatttatcat tccaacactg aatagctgga ctactgtttc ctctaaataa
      361 attgtacccc ccagaactgg attctaaaat actctctatc atcaagaagg cagtgacaat
      421 tagaacgaca cacacatatg aatatcaata ctcactgcta tttggtgatg caggctatct
      481 atagttactc ctacatttat tttctatcag taaaaatcaa tactatctac aattagcaaa
      541 cg
//
LOCUS       DAVLSL010000284          537 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.286, whole genome shotgun sequence.
ACCESSION   DAVLSL010000284 DAVLSL010000000
VERSION     DAVLSL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.286"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>537
                     /locus_tag="VXN30_002137"
     CDS             <1..>537
                     /locus_tag="VXN30_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1358668.1"
                     /translation="ILSNEDLGATNARNIGIKNSRGKYISFLDDDDEYMPDRILKLMA
                     CFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEV
                     LELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINA
                     DEEYYNYCRKYFNLLSFNE"
BASE COUNT          198 a           57 c          109 g          173 t
ORIGIN      
        1 attttaagta atgaagattt aggtgcaact aatgccagaa acataggtat caaaaattct
       61 agaggtaagt atatatcatt tttagacgat gatgatgaat atatgccaga tcgaatttta
      121 aagttgatgg cttgttttaa aaagagtaga atgaagaatt tagctttagt ttatagttat
      181 ggaataataa tttatccaaa tggtacacga gaagaggaga agaccgattt tgttggaaat
      241 cccttgtttg ttcaaatggt tcacaatata gcaggtacgt cattttggtt gtgtaaaaaa
      301 gaggtgctag aattaattaa tggttttgag aaaatagatt cacatcagga cggtgttgtt
      361 ttattaaaac tacttgctca aggataccaa attgatatag tgcgagaatt cttggtgaat
      421 tactacgctc acagtaaaga aaacggtatc actggagtga cacaaaaaac aattaatgca
      481 gatgaagaat attataatta ctgtaggaaa tattttaatt tattgagttt caacgag
//
LOCUS       DAVLSL010000285          531 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.287, whole genome shotgun sequence.
ACCESSION   DAVLSL010000285 DAVLSL010000000
VERSION     DAVLSL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.287"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(156..452)
                     /locus_tag="VXN30_002138"
     CDS             complement(156..452)
                     /locus_tag="VXN30_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV1358669.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          195 a           97 c           66 g          173 t
ORIGIN      
        1 aaaaaaagta aaaataattt cccttgaaaa ggaagatagc ttttaaaata gattgacaaa
       61 cttatgataa caaaaaacaa aagggttatc ccaataaaat taagataacc taaaatactt
      121 tcacattctt ttttagaaag tattttcatc tagttctacc atagagctgc tttagcagca
      181 cctttctttt tcaatatata aagaactgtt gctgcaatac ccgaaccaat agaacctact
      241 cctgtgattg cacctacaat tgaaattact gctgtaactg tactataagc actaatcaaa
      301 ttgactacag tagcagcagc ttttgtcgaa attcctaatg tcccagccaa cataggaaaa
      361 gaaacaacac ctaagcttaa ccaactaatg actgctaaca ttgctgaaac aataagaaat
      421 ttattatgaa attccattct tttagatttc attttagtgt actccttttg tatattatgt
      481 atgttttatt gagagttaat tcgcacatgt tgtattccta actctcttca a
//
LOCUS       DAVLSL010000286          531 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.288, whole genome shotgun sequence.
ACCESSION   DAVLSL010000286 DAVLSL010000000
VERSION     DAVLSL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.288"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..415)
                     /locus_tag="VXN30_002139"
     CDS             complement(<1..415)
                     /locus_tag="VXN30_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1358670.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
BASE COUNT          170 a           92 c           82 g          187 t
ORIGIN      
        1 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
       61 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
      121 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
      181 tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga
      241 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
      301 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccggac
      361 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
      421 ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa
      481 aggttaattt gttgtttttt gctttttatt ttggtagaat aaaacggagg t
//
LOCUS       DAVLSL010000287          529 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.289, whole genome shotgun sequence.
ACCESSION   DAVLSL010000287 DAVLSL010000000
VERSION     DAVLSL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.289"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            34..>529
                     /locus_tag="VXN30_002140"
     CDS             34..>529
                     /locus_tag="VXN30_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1358671.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEEL"
BASE COUNT          189 a           62 c          113 g          165 t
ORIGIN      
        1 tgtaaaatcc cgatattccg aggaggtggt tgcatgaaga aagtgaaatt agggcaagtg
       61 gctactttta tcaatggcta tgcttttaaa cctcaagatt ggtcatctga agggaaagag
      121 attatcagaa tacagaatct gactaaaact tcaaaaggga taaattatta ttcgggtacg
      181 attgataaaa aatacattgt tgaagctggt gatattctta tttcgtggtc gggtacatta
      241 ggggtttttc agtggtgtgg aagatccgca gtattaaatc agcacatttt taaagtggtt
      301 tttgataaga tagatataga taaatcgtat tttaaatatg ttgttgaaaa aggtttacaa
      361 gatgctgtaa aacatactca tggttccaca atgaaacatt taactaaaaa atattttgat
      421 aatattatag ttccatatac aaatttggga gagcaacaaa gaattgcaag tgagctggat
      481 ttgttaagca agctgatttt aaggagacaa gaacaacttg aagagctta
//
LOCUS       DAVLSL010000288          516 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.290, whole genome shotgun sequence.
ACCESSION   DAVLSL010000288 DAVLSL010000000
VERSION     DAVLSL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.290"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..415
                     /locus_tag="VXN30_002141"
     CDS             <1..415
                     /locus_tag="VXN30_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1358672.1"
                     /translation="EEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLYK
                     PSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLTG
                     LKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIP"
BASE COUNT          196 a           85 c          108 g          127 t
ORIGIN      
        1 cgaggagaaa cctcagacag aaaaacctga ggaagataag gaacatgatg aagtaagtga
       61 gccaactcac cctgaatctg atgaaaaaga gaatcacgct ggtttaaatc cttcagcaga
      121 taatctttat aaaccaagca ctgatacgga agagacagag gaagaagctg aagatactac
      181 agatgaggct gaaattcctc aagtagagca ttctgttatt aacgctaaga tagcagatgc
      241 ggaggccttg ctagaaaaag taacagatcc tagtattaga caaaatgcta tggagacatt
      301 gactggtcta aaaagtagtc ttcttctcgg aacgaaagat aataacacta tttcagcaga
      361 agtagatagt ctcttggctt tgttaaaaaa aagtcaaccg gctccgatac cgtagtaaaa
      421 tgaatggagc atattttatg gagaagtaac ctttcgtgtt acttctcttt tttagaaaaa
      481 cgtaacagaa acttgacaaa agtaatttta aatagt
//
LOCUS       DAVLSL010000289          497 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.291, whole genome shotgun sequence.
ACCESSION   DAVLSL010000289 DAVLSL010000000
VERSION     DAVLSL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.291"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..>497)
                     /locus_tag="VXN30_002142"
     CDS             complement(<1..>497)
                     /locus_tag="VXN30_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358673.1"
                     /translation="SLIILATLISTAYFLETLKLKYLCHLILGWFTQIWITQGRIEMI
                     VLLISTIVCIIVQGRLTRKKIIYWLLTILGLWMITLTPIFENIVGAILKVDGAGRGSD
                     SLTIRAIGRQLYWDQLNETTSKLLFGTGYPNYNYALAFQRTGFSSNIFLTDNGFMTYI
                     YIFGI"
BASE COUNT          185 a           88 c           62 g          162 t
ORIGIN      
        1 aataccaaat atatatatat atgtcataaa accattgtct gttaaaaaaa tattgcttga
       61 aaaacctgta cgttgaaagg ctaaagcata attataatta ggatatcctg ttccaaaaag
      121 aagttttgaa gttgtctcat tcaattgatc ccaatataac tgtcgtccaa ttgctcgaat
      181 agtcaatgaa tcacttcctc tacctgctcc atctactttt aaaatagctc caacaatatt
      241 ttcaaaaata ggtgttagag taatcatcca cagtcccaaa attgtcagca accaataaat
      301 tattttcttt ctagttaatc ttccttggac tattatgcaa actatcgttg aaattagcaa
      361 aacaatcatc tctattcgac cttgcgttat ccaaatttga gtaaaccaac ctaatataag
      421 gtggcacaga tacttgagtt ttaatgtttc taaaaaataa gcagtagaaa ttaatgttgc
      481 taaaataatt aaagaac
//
LOCUS       DAVLSL010000290          497 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.292, whole genome shotgun sequence.
ACCESSION   DAVLSL010000290 DAVLSL010000000
VERSION     DAVLSL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.292"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..497
                     /locus_tag="VXN30_002143"
                     /pseudo
     CDS             1..497
                     /locus_tag="VXN30_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          177 a           94 c           89 g          137 t
ORIGIN      
        1 ctaaaccacc aagtaaaagg aacaaaacca agaaaggttg atagagatag acttaaaaac
       61 tatcttactg acaatccaga cgcttatttg actgaaatag cttctgaatt tgcctgtcat
      121 ccaactacca tccactatgc gctcaaagct atgggctaca ctcgaaaaaa agaaccacac
      181 ctactatgaa caagacccag aaaaagtagc cttatttctt aaaaatttta atagtttaaa
      241 gcacctagca cctgtttaga ttgatgaaac aggattcgat acttattttt atcgagaata
      301 tggtcgctca ttaaaaggtc agttaagaag aggtaaagta tctggaagaa gatatcagag
      361 gatttctttg gttgcaggtc taacaaatgg tgagttaatc gctccaatga cttacgaaga
      421 gatgatgacg agcgactttt ttgaagcttg gtttcagaag tttctcttac caacattaac
      481 cacaccatcg gttatta
//
LOCUS       DAVLSL010000291          483 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.293, whole genome shotgun sequence.
ACCESSION   DAVLSL010000291 DAVLSL010000000
VERSION     DAVLSL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.293"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(153..>483)
                     /locus_tag="VXN30_002144"
     CDS             complement(153..>483)
                     /locus_tag="VXN30_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1358674.1"
                     /translation="EEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEESPEESEEPQ
                     VETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLL
                     ALLKESK"
BASE COUNT           96 a          101 c           84 g          202 t
ORIGIN      
        1 tttagaagta gaggtatact attctagttt caatctacta tattttgcac attttcatga
       61 aaaaaatgag aactagaact cacattctgc tctcattttt cgttttcccg ttctcctatc
      121 ctgtttttag gagttagaaa atgctgctac ctttacttac tctcctttaa taaagccaat
      181 agtttttcag cttctgccat aatagtattg ttgtcctggg tgccaaatag taaattattt
      241 tttaatcctg tgagagtctc tttggcattg gacttgataa ttggatcctg gatttttcca
      301 agtaaatctt cagcctctct cagtttttct tcaacctttt cagtctcgac ctgaggttct
      361 tctgattcct ctggtgattc ttctggttcc tctgttggtt ttggagactc tggtttctcg
      421 ctttgcggtt tctcttctcg aggggtttct tcctcaggtt tttctgtctg aggtttctcc
      481 tcg
//
LOCUS       DAVLSL010000292          480 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.294, whole genome shotgun sequence.
ACCESSION   DAVLSL010000292 DAVLSL010000000
VERSION     DAVLSL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.294"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>480
                     /locus_tag="VXN30_002145"
     CDS             <1..>480
                     /locus_tag="VXN30_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1358675.1"
                     /translation="QIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRC
                     LIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEE
                     KAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKTSPFVFFDEPSSGMDYSNM"
BASE COUNT          183 a           62 c           94 g          141 t
ORIGIN      
        1 atcagataaa aaatcttagc tacaaattta ctgatgatga gtgtttaagc ttaaaagata
       61 tttcattcaa gcttgggaaa atttatggca taataggatc caacggacga ggaaaatcaa
      121 cgcttttaag atgtttaata ggtcttgaga aaaaatcaaa agaagaaatt tattttaagg
      181 gagagaagct atctaaaaaa gaaagactca aaaactcttc acttgttatg caagatgtaa
      241 atcatcaatt attcacagat gaagtattca acgagcttag attaggagta aagaattttg
      301 atgaagaaaa ggcgaaaatc attttaaaag atttaggcct ggacgaattt attgaaaggc
      361 atccgatgag tttatcagga gggcaaaagc aaaggcttgc aatagcatct gttatgtgca
      421 agacttctcc atttgtcttt tttgacgaac cttcaagtgg tatggattat tccaatatga
//
LOCUS       DAVLSL010000293          470 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.295, whole genome shotgun sequence.
ACCESSION   DAVLSL010000293 DAVLSL010000000
VERSION     DAVLSL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.295"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            28..198
                     /locus_tag="VXN30_002146"
     CDS             28..198
                     /locus_tag="VXN30_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358676.1"
                     /translation="MKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQLEIRVQSQ
                     KKSNGLDVGKAD"
BASE COUNT          182 a           52 c           93 g          143 t
ORIGIN      
        1 acaaggaaaa agaaaagggt ttcacgaatg aagaaaaatc gtggaattca aaaattagct
       61 atattagtat tactaggtgt ttttatgttt agtaacacaa ttccttacca acagtttatt
      121 cagaagaata gacaattgga gattcgagtg caatcacaaa agaagtccaa tggtcttgat
      181 gttgggaagg ctgattgatt tcgtattact ttaaaattca actatttaga gaaaggtatt
      241 ctgatgagag cttgtaacaa taccagatag atataagaat ggagatgttt gataaaatgc
      301 tttcatagct aaccaactgt tgaaaattat gagttacaaa atctaaagta tttaagattg
      361 gtttataaaa ttaggtctct gcctgtagaa aggaacgtgc agttagaaag gaggaaaaaa
      421 taggatacta taattcagac tgagatttct aattaaaata tgtagttgca
//
LOCUS       DAVLSL010000294          452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.296, whole genome shotgun sequence.
ACCESSION   DAVLSL010000294 DAVLSL010000000
VERSION     DAVLSL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.296"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
BASE COUNT          177 a           88 c           55 g          132 t
ORIGIN      
        1 agtccaataa ttggactagc agacatgctt agatataata aaaataacaa aaaatgaatt
       61 tcaccaattc ctgcaaaaaa atgtttcata gctatagttt tacgcccata aatacccatc
      121 aaatttaccg tctacgatat ctaaaccata tgcaatggct cctcctaatg agaagacttc
      181 agcaacagta gccgcaatat taagagcatt agtaatagtt gccatactaa ctccaccaat
      241 atatcttgtt atagcactta gttacctaga taagatatcc tttagcttgc ttccaaaatt
      301 taagtctaca cctataatat gagttcatat tagtactgct aaatcctatc tatagtaatg
      361 tcaaacacct aaataaagtc atctctccaa ttcattcaag aaatgaagaa aggcaaaaaa
      421 tctttaaaat aagaaaagtg catattatca gg
//
LOCUS       DAVLSL010000295          440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.297, whole genome shotgun sequence.
ACCESSION   DAVLSL010000295 DAVLSL010000000
VERSION     DAVLSL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.297"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
BASE COUNT          180 a           76 c           76 g          108 t
ORIGIN      
        1 tctaaagatg aaataacata attgtccctt ggttacaagg gcgcaattat acaccctaaa
       61 gggtgcttac gtcctgcgga gtcaaaccct tgcagggagc aacaaccatt cgctttttaa
      121 aagtcaaggg taaataaact cgctaaggct cgcccttgac ttttaaaatt tgaaatgaaa
      181 gtaacaatta agccgacata ctgaaacaac aaaaattaat tcagtagtcg gcttttttta
      241 ttctatcatt tcaaaacgct cattcacaaa gaggatataa aaaaactatt aagcctccac
      301 ctaaaacaac aaagaaagaa aggaagtgat aaaaatggca gacagttttc atttttcagt
      361 aaacataata tcaagaggaa aaggcaaaag tgcagtagca agtgcagcat atataagtgg
      421 agaaaaaata aaaaatgaat
//
LOCUS       DAVLSL010000296          421 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.298, whole genome shotgun sequence.
ACCESSION   DAVLSL010000296 DAVLSL010000000
VERSION     DAVLSL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.298"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(70..274)
                     /locus_tag="VXN30_002147"
                     /pseudo
     CDS             complement(70..274)
                     /locus_tag="VXN30_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
BASE COUNT          115 a           93 c           72 g          141 t
ORIGIN      
        1 aaaaatcctt atcttttatt ttatcacgtt ccaaggattt tctcaagttg gaggaagggg
       61 acaacatctc tactttccct tcaaaaatcc ttccaaatca tgtttatgtt ggtaattaac
      121 ggctgcggtt ttgtctttct caaagacagt cttggtaagg tcaatatgat taatagctac
      181 gattgcgacg gtgtagtaaa tgatatcagc cagttctctg gcaagttcct cgttcgaatc
      241 ctatcccttc ttttcgacca gagcgcctat tcaaaacctc ggctacttct ccgacttctc
      301 cactaacttc ataaagagac cttcatcagt ccgagactgc tgttaatgtt cgagtaagta
      361 gtcttggaat tgcctaaacg ttcaatcttt tatagtatat tgaaactaga atagtacacc
      421 t
//
LOCUS       DAVLSL010000297          416 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.299, whole genome shotgun sequence.
ACCESSION   DAVLSL010000297 DAVLSL010000000
VERSION     DAVLSL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.299"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
BASE COUNT          134 a           84 c           74 g          124 t
ORIGIN      
        1 caaggtgctg gagttccatg gactactgtt tctgacctca gaaaaggaaa aacaagcatg
       61 gacaaaatgg cacttctcac agcagaaaaa ctttatgaat ttgctacaac tgataagcag
      121 tgatttcggt cactgttttt atttttgaca aaacaccgtt tttgacaata atcaaaaaat
      181 aaaaaggagc tattattaac aaaatggcgt ttttggcaat aataccctgc cattcctcct
      241 ccctattctt caagaaaacg cttttttgaa gaatcagagc aaataaaaaa ccgcaagcct
      301 gagcctgcgg tgaaagaaca atttagaaag tttcctttct atttatttaa ctgtaatcaa
      361 gccatctggc tctactgtga actctggctt gtctgccagt gttccgtctg gtttga
//
LOCUS       DAVLSL010000298          415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.300, whole genome shotgun sequence.
ACCESSION   DAVLSL010000298 DAVLSL010000000
VERSION     DAVLSL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.300"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..>415)
                     /locus_tag="VXN30_002148"
     CDS             complement(<1..>415)
                     /locus_tag="VXN30_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1358677.1"
                     /translation="DKGIYRVEGENGSGKSTLLQLLAGLETFDSGLKNCVSKDVLYLD
                     TNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINTFFDNKIGDNYMTASTGTKFKLG
                     LSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM"
BASE COUNT          143 a           79 c           55 g          138 t
ORIGIN      
        1 catggaaatt aagcgtttag caatcatatc aatacttctt gcatccactg ttgataaagt
       61 ctcatcaata aaaatataat cccaattttt agcaaaaatt aaggataagc ctaatttgaa
      121 ttttgttcca gtagatgctg tcatatagtt atctccgatt ttattatcaa aaaaagtatt
      181 aatttgttct ttatcctcaa atgatagatg gatttgaaaa gaatcacaca acaactgcaa
      241 attttcttct atagtaaatg gaacaacccc aagctgattg gtatcaaggt atagtacatc
      301 tttggagaca caattcttta aaccactatc aaaagtctct agcccagcta aaagttgtag
      361 aagggtgctc tttcctgaac cattctctcc ttcaactctg taaattccct tatca
//
LOCUS       DAVLSL010000299          412 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.301, whole genome shotgun sequence.
ACCESSION   DAVLSL010000299 DAVLSL010000000
VERSION     DAVLSL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.301"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>412
                     /locus_tag="VXN30_002149"
     CDS             <1..>412
                     /locus_tag="VXN30_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEV1358678.1"
                     /translation="YIDFNPTDSRNPADYRWTRWKGRDGANGVAGRAGADGRTPYLHI
                     AYATSNNGSQGFSTTDSTNKTYIGTYTDYTQADSTDPKKYKWAKVKGDKGEKGDKGEP
                     GQRGLQGLQGARGEQGIPGPRGADGRTQYTHMAYA"
BASE COUNT          144 a           85 c          105 g           78 t
ORIGIN      
        1 tgtacattga ctttaatcca acagacagca gaaatcctgc tgattatcgc tggacaagat
       61 ggaaaggtcg tgatggcgca aatggcgtgg caggtagggc tggtgcagat ggtaggacac
      121 catacttaca catagcttac gccacatcaa ataatggatc acaaggcttc tcaactactg
      181 acagtacaaa taaaacgtat atcggaacat acacagatta cactcaggca gatagtaccg
      241 accctaaaaa atacaagtgg gctaaagtaa aaggggacaa gggagaaaaa ggcgataaag
      301 gagaaccagg acaacgtggt ttacaaggct tgcaaggtgc acgaggtgaa caaggtattc
      361 ctggacctag aggggcagat ggccgcacac aatatactca catggcctat gc
//
LOCUS       DAVLSL010000300          400 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.302, whole genome shotgun sequence.
ACCESSION   DAVLSL010000300 DAVLSL010000000
VERSION     DAVLSL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.302"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..89
                     /locus_tag="VXN30_002150"
     CDS             <1..89
                     /locus_tag="VXN30_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEV1358679.1"
                     /translation="NLDTIVSDSKLSDSILFKLSKHIKVIKP"
BASE COUNT          141 a           68 c           58 g          133 t
ORIGIN      
        1 caaatcttga tactattgtt tcagattcta aactaagtga ttcaatcctt tttaagctat
       61 ctaaacacat taaagtcatc aagccttaat aaactgaaag agcctgggta acaactcaat
      121 ttcagaagaa aaaaagtaaa tcttcccaca ataaaaagca tagtatcagg tgtttcaaca
      181 cctgatacta tgcttttttc tatatcaaag actttttgcc caattcctca aagctatgca
      241 ttgagaaatc tgcgactcgc ttcctagttt aaagttgctc tttgattttc attgagtata
      301 agcaacgtga ttagataaga gttttacaga gtactaaata taagatttaa cttttttgag
      361 aaattggtta cgattgttga aacggcataa aatctgaatc
//
LOCUS       DAVLSL010000301          394 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.303, whole genome shotgun sequence.
ACCESSION   DAVLSL010000301 DAVLSL010000000
VERSION     DAVLSL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.303"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>394
                     /locus_tag="VXN30_002151"
     CDS             <1..>394
                     /locus_tag="VXN30_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1358680.1"
                     /translation="DDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDG
                     YIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQD
                     SGNTEAKGAEAIYNRVKAAKKVPLDRMP"
BASE COUNT          125 a           85 c           92 g           92 t
ORIGIN      
        1 tggatgatat tcttgccttc ttagctccga ttcgtcatcc agaacgttta ggaaaaccaa
       61 atgcgcaaat tacctacact gatgatgaga ttcaagtagc caagttggca ggcaagtaca
      121 caacagaaga cggttatatc tttgatcctc gtgatataac cagtgatgag ggggatgcct
      181 atgtaactcc acatatgacc catagccact ggattaaaaa agatagtttg tctgaagctg
      241 agagagcggc agcccaggct tatgctaaag agaaaggttt gacccctcct tcgacagacc
      301 atcaggattc aggaaatact gaggcaaaag gagcagaagc tatctacaac cgcgtgaaag
      361 cagctaagaa ggtgccactt gatcgtatgc ctta
//
LOCUS       DAVLSL010000302          386 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.304, whole genome shotgun sequence.
ACCESSION   DAVLSL010000302 DAVLSL010000000
VERSION     DAVLSL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.304"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            25..363
                     /locus_tag="VXN30_002152"
     CDS             25..363
                     /locus_tag="VXN30_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358681.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
BASE COUNT          138 a           49 c           74 g          125 t
ORIGIN      
        1 tatttaaatt caaggagaag taaaatgaaa aaaagaatta ataagaaagc atctgttttt
       61 ctagttgcaa gtgttttcag ttttagcttt gctactacag ctagtgtcgt taacgcagta
      121 acggcacacc ctgctccaag tcccattatt gatgtatggg aatatggtgt aaatgataac
      181 tatggttatt cgaattactt tgttagttct ccaagtaata ttggatcaaa atcttcagta
      241 acgaattttt ggggaacagt taaagctcag gataaaaaag attatggatg ggcatattca
      301 agtgcaacaa aatcttggaa cgatgttcgc ttaaatgcat attgggatta ttatagattt
      361 taaaaataat ttatgaaaac aagaga
//
LOCUS       DAVLSL010000303          383 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.305, whole genome shotgun sequence.
ACCESSION   DAVLSL010000303 DAVLSL010000000
VERSION     DAVLSL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.305"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            1..383
                     /locus_tag="VXN30_002153"
                     /pseudo
     CDS             1..383
                     /locus_tag="VXN30_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          153 a           60 c           79 g           91 t
ORIGIN      
        1 gagagtacct aaaatgagac gtaagccaaa aaagagacct gaagagagga cagaattaga
       61 acgtcttcaa gcagaaaatg agtacctgag agcggagaag gccatcctaa aaaagttaag
      121 agaactccga ttgaaggagg aaaaagagaa agaagaaaga cagaaattgt ttaagaatta
      181 atgactgagt tttcgttagc tcttcttcta aaatccatta aactagctcg ttggacctac
      241 tactatcact tgaaacagct agataaacca gataaggacc aagagcttaa agctgaaatt
      301 caatccattt ttatctaaca caagggaaat tatgcttatc gtcggattta tttagaacta
      361 agaaatcgtg gttatctggt aaa
//
LOCUS       DAVLSL010000304          369 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.306, whole genome shotgun sequence.
ACCESSION   DAVLSL010000304 DAVLSL010000000
VERSION     DAVLSL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.306"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(<1..>369)
                     /locus_tag="VXN30_002154"
     CDS             complement(<1..>369)
                     /locus_tag="VXN30_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1358682.1"
                     /translation="EEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIALARA
                     LINNPSIVILDEATSALDTINEERITKYIKSQGCTQIIVAHRLSTIKDADIIFVMKGG
                     KIVESGNHKYLMDLGGEYYSL"
BASE COUNT          108 a           84 c           45 g          132 t
ORIGIN      
        1 taagctgtag tactctccac caagatccat taagtactta tgatttcctg actcaacaat
       61 cttaccacct ttcattacaa aaataatatc cgcatcctta atcgttgaca atctatgagc
      121 tacaattatt tgagtacagc cctgactttt tatatacttt gttattcttt cctcattaat
      181 agtgtctaaa gcactagttg cttcatctaa aattacaata ctaggattat ttattaatgc
      241 acgtgccagt gctatccgtt gcctttgccc acctgaaata tttgacccca tctctgagat
      301 gatagtatta aatttcatcg gcatagccat gatttcatca tagatttgaa ctgctttaca
      361 aacttcctc
//
LOCUS       DAVLSL010000305          367 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.307, whole genome shotgun sequence.
ACCESSION   DAVLSL010000305 DAVLSL010000000
VERSION     DAVLSL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.307"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            52..>367
                     /locus_tag="VXN30_002155"
     CDS             52..>367
                     /locus_tag="VXN30_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1358683.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHK"
BASE COUNT          144 a           48 c           65 g          110 t
ORIGIN      
        1 taaaatttca aatgaaaaaa gctttttaca tagtaaaatg aggaggaatt tatggaaatt
       61 attgttccag atcaaattat catgggttta attttatatg ctggtgatgc gaaacaacat
      121 atttataaag cgttagatta cataaaaaat ggtacatgtg aacggtgtga agaagaaatc
      181 cagttagctg atgcagcctt attagaagct cataatctac aaacaaaatt cttggcacag
      241 gaagcgtctg gtacaaagac agaaattaca gctctctttg ttcattcaca agatcatctc
      301 atgactagta tgacggagat taatttaatc aaagaaatta ttagtttgag aaaagaactt
      361 cataaaa
//
LOCUS       DAVLSL010000306          319 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.309, whole genome shotgun sequence.
ACCESSION   DAVLSL010000306 DAVLSL010000000
VERSION     DAVLSL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.309"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
BASE COUNT          105 a           52 c           49 g          113 t
ORIGIN      
        1 caaaaacttc ttgtcgatat aagaatatct gagatagtat tttaaaacga aaggactata
       61 aaagctcatt tccttcgcac ttattttata gagatctatc attatactct ttagaaatca
      121 aaaagcaaca caccttgtca tcgttttatc gtaaatctgt taatgagggg gctagtctta
      181 tctgcaatct caaatcagtg ttttgagctg aatggcactc gttttctagt ttgctctttg
      241 attttaattg ggccttagta aatactactc ttaaattaaa ctatagtgaa ttgaaataag
      301 atgtgaacaa ctctatcag
//
LOCUS       DAVLSL010000307          314 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.310, whole genome shotgun sequence.
ACCESSION   DAVLSL010000307 DAVLSL010000000
VERSION     DAVLSL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.310"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            109..306
                     /gene="blpI"
                     /locus_tag="VXN30_002156"
     CDS             109..306
                     /gene="blpI"
                     /locus_tag="VXN30_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV1358684.1"
                     /translation="MNTKMMSQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
BASE COUNT          122 a           37 c           68 g           87 t
ORIGIN      
        1 aattcaagat ctggatgaaa aattttaaaa aacgatgata tactaaactt atcaaagtta
       61 caacaagaca aaaataaaaa aagaaaaaag aaaaaaggag tatttgtcat gaatacaaaa
      121 atgatgtcac aattttctgt tatggataat gaagaactcg aaatagttag tggaggaaga
      181 ggcaatttag gatctgcaat cggtggttgt attggagcag tactattagc tgctgcgact
      241 ggtccgataa ctgggggagc agcaacactt atttgtgtag gttcaggaat tatgtcctct
      301 ttgtaagatt atga
//
LOCUS       DAVLSL010000308          308 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.denovo.311, whole genome shotgun sequence.
ACCESSION   DAVLSL010000308 DAVLSL010000000
VERSION     DAVLSL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.denovo.311"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..>308
                     /locus_tag="VXN30_002157"
     CDS             <1..>308
                     /locus_tag="VXN30_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358685.1"
                     /translation="AYIVEEKSTIKEVSDKQSIAGSPYSIGVYDRLTSPSWKYPSMVL
                     PLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTDLNDYVEKKAKEAAVRFRDHYD
                     "
BASE COUNT          114 a           41 c           62 g           91 t
ORIGIN      
        1 aagcatatat tgtagaagaa aaatctacta ttaaagaggt aagtgataag caatcaatag
       61 ctggtagtcc atattcaatt ggagtatatg atagattaac tagcccatct tggaaatatc
      121 caagtatggt gttgccgcta ttaacattac ctgaaaaatc ggtgtttatt atagccaaca
      181 tttcgactat aggtttcggt gcttatgata gatatagaag taaagaacat ccagcaggaa
      241 ctgatttaaa tgactatgtt gagaaaaaag ctaaagaagc ggctgttaga tttagagatc
      301 actatgat
//
LOCUS       DAVLSL010000309        14775 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.guided.037, whole genome shotgun sequence.
ACCESSION   DAVLSL010000309 DAVLSL010000000
VERSION     DAVLSL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.guided.037"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            <1..1037
                     /locus_tag="VXN30_002158"
     CDS             <1..1037
                     /locus_tag="VXN30_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1358686.1"
                     /translation="SLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWKETLPEIS
                     HEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGERFPILRKA
                     IRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGYVEQSISL
                     EKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRSIFKIVDL
                     DEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGLKFAFRST
                     DLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVFEELLHAF
                     DTRPPVRKEV"
     gene            1040..3217
                     /locus_tag="VXN30_002159"
     CDS             1040..3217
                     /locus_tag="VXN30_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1358687.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            3214..4215
                     /locus_tag="VXN30_002160"
     CDS             3214..4215
                     /locus_tag="VXN30_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEV1358688.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            4212..5147
                     /locus_tag="VXN30_002161"
     CDS             4212..5147
                     /locus_tag="VXN30_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV1358689.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWV
                     LLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKS
                     IDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQ
                     LITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSI
                     TTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVT
                     VSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5392..5508
                     /locus_tag="VXN30_002162"
     CDS             5392..5508
                     /locus_tag="VXN30_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV1358690.1"
                     /translation="MILKYPENICMLFMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5524..7443
                     /gene="tet(M)"
                     /locus_tag="VXN30_002163"
     CDS             5524..7443
                     /gene="tet(M)"
                     /locus_tag="VXN30_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV1358691.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDRGTTKTD
                     NTLLERQRGITIQTAITSFQWKNTKINIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKIGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     HPNMRVMNFTESEQWDMVIEGNDYLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQKYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7562..7729
                     /locus_tag="VXN30_002164"
     CDS             7562..7729
                     /locus_tag="VXN30_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEV1358692.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(7789..8142)
                     /locus_tag="VXN30_002165"
     CDS             complement(7789..8142)
                     /locus_tag="VXN30_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1358693.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            8647..9069
                     /locus_tag="VXN30_002166"
     CDS             8647..9069
                     /locus_tag="VXN30_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1358694.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            9066..9296
                     /locus_tag="VXN30_002167"
     CDS             9066..9296
                     /locus_tag="VXN30_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1358695.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            9757..9960
                     /locus_tag="VXN30_002168"
     CDS             9757..9960
                     /locus_tag="VXN30_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEV1358696.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            10042..11259
                     /locus_tag="VXN30_002169"
     CDS             10042..11259
                     /locus_tag="VXN30_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1358697.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(11421..12023)
                     /locus_tag="VXN30_002170"
     CDS             complement(11421..12023)
                     /locus_tag="VXN30_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1358698.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLFNKNKV"
     gene            complement(12116..12259)
                     /locus_tag="VXN30_002171"
     CDS             complement(12116..12259)
                     /locus_tag="VXN30_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358699.1"
                     /translation="MAERFWENLSIILAERNISCIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            complement(12272..12652)
                     /locus_tag="VXN30_002172"
     CDS             complement(12272..12652)
                     /locus_tag="VXN30_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1358700.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            complement(12645..12992)
                     /locus_tag="VXN30_002173"
                     /pseudo
     CDS             complement(12645..12992)
                     /locus_tag="VXN30_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12979..13230)
                     /locus_tag="VXN30_002174"
                     /pseudo
     CDS             complement(12979..13230)
                     /locus_tag="VXN30_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(13230..13400)
                     /locus_tag="VXN30_002175"
     CDS             complement(13230..13400)
                     /locus_tag="VXN30_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358701.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            complement(13587..13955)
                     /gene="rplL"
                     /locus_tag="VXN30_002176"
     CDS             complement(13587..13955)
                     /gene="rplL"
                     /locus_tag="VXN30_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1358702.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            complement(14031..14531)
                     /locus_tag="VXN30_002177"
     CDS             complement(14031..14531)
                     /locus_tag="VXN30_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1358703.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     misc_feature    complement(14570..14705)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT         4838 a         2732 c         2878 g         4327 t
ORIGIN      
        1 gttcgctggg tggtggtaaa tcctttgcga ataaccttat cgtctattat gcggtgcttt
       61 atggggcaca agcagtgatt gtagacccaa aagcagaacg tggcagatgg aaagaaacct
      121 tgccagagat ttcccatgaa atcaatatcg tcactctgac ttctgatgag aaaaacaaag
      181 gcttacttga cccttatgtg attatgaaaa atcccaaaga ttctgaatca ctggctattg
      241 atattctgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      301 gaaaagccat tcgtgcagta accaatagtg aagtacgagg gttgatgaaa gtgattgagg
      361 aattacgggt tgagaatacg ccactaagta ccagtatagc cgaccatatc gaaagtttta
      421 cagactatga ctttgcacat ttattattca gtaatggtta tgtggagcag tctatcagct
      481 tagaaaaaca actgaacatt atacaggttg cggacttggt acttcccgac aaggaaactt
      541 cctttgagga atataccact atggagcttt tatccgttgc tatgctgatt gtcattagta
      601 cctttgcttt agactttatc catacagacc gaagcatttt caagattgta gatttagacg
      661 aagcatggag ctttttacag gtagcacaag gaaaaacact atctatgaag ctggttcggg
      721 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagac gacctcttag
      781 atgaaaaact gaaaaataac ctcggcttaa aatttgcatt tcgttccact gaccttaacg
      841 agattaaaaa gaccttagcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      901 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      961 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
     1021 tgcgaaaaga ggtgtaaatg tgaaaccatc aatagtaaac agaataaaat caaactggac
     1081 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1141 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1201 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1261 taatagctgg ggctggcttc cgtggaactg gtcggacggg attggaaaac aggtcatgta
     1321 tggactatat gccattacca attttatttg gacaatcagt ttgtatgttt ccaatgcgac
     1381 aggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1441 tggtaagaat atgcagacct tagctggtgt gagtgcaaac ggattttcaa cagagggttt
     1501 ctatgttgga ttcctcttac tcttgatttt ggttcttggg gtttatgttg cctatacggg
     1561 actgataaag agagaaacca caaaggcaat tcatgccatt atgaattttg tgctggtgtt
     1621 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1681 ttcatcagac atcagtaatg ccagtttatc acttggcacg aagattgtca tgccccattc
     1741 cgatagtcaa ggcaaggaca gcgtggactt aatcagagat agcctgtttt ccatacaggt
     1801 tcagcaaccg tggctactgc ttcaatacaa cagttcagac attgaaagta tcggtattga
     1861 ccgtgtggaa agcctgctct ccaccagccc agattccaac aatggcgaag acagagaaaa
     1921 aattgttgcg gaagaaattg aagacagaag caataccaat ctaaccatta caaagaccat
     1981 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     2041 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2101 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2161 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2221 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2281 tgcttttcta cagattgtga cctttgcagg aatctacttc aagctgggcg atttaatgag
     2341 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtgtga tgagaaaacc
     2401 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gttccatgac
     2461 tactctaggg gctgggtctg ccattgttac aggtaaaaaa ggacagtcgg gttcggggag
     2521 ttctgcaagg acacaagcag atcactcccg accagacgga aaggaaaaat caacacttgg
     2581 aaaacgtatc ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagacac
     2641 tgctagtggt ttgaaagaac aggttaaaga tttgccgacc aatgcaagat atgcagtata
     2701 tcaaggaaaa tccaaagtaa aagagaatgt ccgtgattta accagtagta tttctcaaac
     2761 caaagcggac agagccagtg gacgcaagga acagcaggaa caaaggcgaa aaaccattgc
     2821 gaagcgtcgc tctgaaatgg aacaggtcaa acagaaaaaa cagcctgctt cttctgttca
     2881 tgaaagaccg actacaagac aagaacaata tcatgatgaa cagacctcaa aacagtctaa
     2941 tattcagact tcatataagg aatctcaaca agccaaacaa gagcgtccag cagttaagtc
     3001 cgatttttca agtccaaaag tggaacgcca aggcaatacc gttcaagaaa aaaccgttca
     3061 aaagccagca acttcaacca ctacagcaga tagaacttca caacgtccaa tcacaaaaga
     3121 acgtccgtct actgttcaaa gagtaccact acaaaataca agaagtagac caccaatcaa
     3181 aaccgccacc attaagaaag tcggtaagaa accatgaagt tgaaaacttt agtgattggt
     3241 ggttctggat tattcttgat ggtcttctca ctgcttctgt ttgttgccat tttattttca
     3301 gatgaacagg acagcggaat ttccaatatt cattatggag gtgtgaatgt ttccgcagaa
     3361 gtgctggctc ataagcctat ggtagaaaaa tatgccaaag aatatggcgt tgaagaatat
     3421 gtcaacatac ttcttgcgat tatacaggtg gaatcgggcg gtactgcgga agatgttatg
     3481 cagtcctcgg aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag
     3541 caaggtgtga agtatttcag tgaattatta gccagtagcg aaaggctcag tgtagattta
     3601 gaatcggtta tccagtccta caattatggt ggtggtttct tagggtatgt ggctaatcgt
     3661 ggaaataaat atacctttga actggctcaa agtttctcaa aagagtattc aggtggcgaa
     3721 aaagtgtctt accccaatcc catagccata cctatcaatg ggggctggcg atacaactat
     3781 ggcaatatgt tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat
     3841 gatacggtac aagccatcat ggacgaagca ctgaaatatg agggctggcg atacgtttac
     3901 ggtggagctt ccccgactac ttcttttgat tgtagcggac tgacacaatg gacgtatgga
     3961 aaagctggaa ttaacttacc acgaaccgca caacagcaat atgatgtgac ccagcatatc
     4021 ccactatcgg aagcacaagc tggcgatttg gttttctttc attctaccta taacgctggc
     4081 tcttatatta ctcatgttgg gatatacctt ggcaataacc gtatgtttca tgcaggcgac
     4141 ccaatcggtt atgccgactt aacaagcccc tactggcaac agcatttagt gggagcagga
     4201 cgaatcaaac aatgagaaag gaagatttaa tgatgaaatt tagaaaaaat cagaataaag
     4261 aaaaacagat accaaaggaa aagaaacctc gtgtctacta taaggtcaat cctcataaaa
     4321 aggttgtgat tgccttgtgg gtacttttag ggcttagttt cagctttgcg atattcaagc
     4381 actttacagc tatagatact catactattc acgaaacaac tatcatagaa aaggaatacg
     4441 ttgatactca tcatgtagaa aattttgtag agaactttgc gaaagtctac tattcatggg
     4501 agcaatccga taagtccatt gataatcgaa tggaaagtct aaaaggctat ctgacagatg
     4561 aacttcaagc tctcaatgtt gatacagtac gcaaagatat tcctgtatcg tcttctgtaa
     4621 gaggatttca gatatggacg gtagagccaa ctggcgacaa tgagtttaat gtaacctaca
     4681 gtgtagacca gctcattaca gagggagaaa atacaaagac cgtccactct gcttatatag
     4741 tgagtgtcta tgtagatggt tctggaaata tggtactggt taagaatccg accattacca
     4801 acatacctaa gaaatcaagt tataaaccaa aagccattga aagtgagggg acggttgatt
     4861 ccattacaac caatgaaatc aatgagtttt taacgacgtt cttcaagctc tatcctacag
     4921 cgacagccag tgaactttcc tactatgtga atgacgggat attaaaacca atcggaaaag
     4981 agtacatctt tcaagaactg gtaaatccta ttcacaatcg taaggataat caagtcacgg
     5041 tatcgctgac agtggagtat atcgaccagc agaccaaagc aacgcaggta tctcaatttg
     5101 atttggtact tgaaaagaac gggagtaatt ggaagattat agaataacaa atattggtac
     5161 attattacag ctattttgta atcacgtact ctctttgata aaaaattgga gattccttta
     5221 caaatatgct cttacgtgct atcattaaag tatctattta aaaggagtta ataaatatgc
     5281 ggcaaggtat tcttaaataa actgtcaatt tgatagtgga aacaaataat tggatgtcct
     5341 tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca tgtgattcta
     5401 aagtatccgg agaatatctg tatgcttttt atgcctatgg ttatgcataa aaatcccagt
     5461 gataagagta tttatcactg ggatttttat gcccttttgg gcttttgaat ggaggaaaat
     5521 cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcgggaaa aactacctta
     5581 acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt ggacagaggt
     5641 acaacgaaaa cggataatac gcttttagaa cgtcagagag gaattacaat tcagacggcg
     5701 ataacctctt ttcagtggaa aaatactaag ataaacatca tagacacgcc aggacatatg
     5761 gattttttag cagaagtata tcgttcatta tcagtattag atggggcaat tctactgatt
     5821 tctgcaaaag atggcgtaca agcacaaact cgtatattgt ttcatgcact taggaaaata
     5881 ggtattccca caatcttttt tatcaataag attgaccaaa atggaattga tttatcaacg
     5941 gtttatcagg atattaaaga gaaactttct gcggaaattg taatcaaaca gaaggtagaa
     6001 ctgcatccta atatgcgtgt aatgaacttt accgaatctg aacaatggga tatggtaata
     6061 gaaggaaatg attacctttt ggagaaatat acgtctggga aattattgga agcattagaa
     6121 ctcgaacaag aggaaagcat aagatttcat aattgttccc tgttccctgt ttatcacgga
     6181 agtgcaaaaa acaatatagg gattgataac cttatagaag tgattacgaa taaattttat
     6241 tcatcaacac atcgaggtca gtctgaactt tgcggaaaag ttttcaaaat tgagtattcg
     6301 gaaaaaagac agcgtcttgc atatatacgt ctttatagtg gcgtactgca tttgcgagat
     6361 tcggttagaa tatcggaaaa ggaaaaaata aaaattacag aaatgtatac ttcaataaat
     6421 ggtgaattat gtaaaatcga taaggcttat tccggggaaa ttgttatttt gcagaatgag
     6481 tttttgaagt taaatagtgt tcttggagat acaaagctat tgccacagag agagagaatt
     6541 gaaaatcccc tccctctgct gcaaacgact gttgaaccga gcaaacctca acaaagggaa
     6601 atgttacttg atgcactttt agaaatctcc gacagtgacc cgcttctgcg atattatgtg
     6661 gattctgcga cacatgaaat catactttct ttcttaggga aagtacaaat ggaagtgact
     6721 tgtgctctgc tgcaagaaaa gtatcatgtg gagatagaaa taaaagagcc tacagtcatt
     6781 tatatggaaa gaccgttaaa aaaagcagag tataccattc acatcgaagt tccaccgaat
     6841 cctttctggg cttccattgg tctatctgta gcaccgcttc cattagggag cggagtacag
     6901 tatgagagct cggtttctct tggatactta aatcaatcgt ttcaaaatgc agttatggag
     6961 gggatacgct atggctgtga acaaggattg tatggttgga atgtgacgga ctgtaaaatc
     7021 tgttttaagt atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt
     7081 gctcctattg tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat
     7141 cttagtttta aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct
     7201 aaatattgtg cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga
     7261 gaaatccctg ctcggtgtat tcaaaaatat cgtagtgatt taactttctt tacaaatgga
     7321 cgtagtgttt gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc
     7381 cagccccgtc gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact
     7441 tagtgtattt tatgttgtta tataaatatg gtttcttgtt aaataagatg aaatattttt
     7501 taataaagat ttgaattaaa gtgtaaagga ggagatagtt attataaact acaagtggat
     7561 attgtgtcct gtatgtggaa ataaaacacg attaaagata agggaagata ctgaattaaa
     7621 aaaattcccc ctctattgtc cgaaatgcag acaagaaaat ttaattgaaa taaagcagtt
     7681 caaagtaact gtgattacag agccagacgc aaagacgcag agccgataaa atgagattaa
     7741 tacaatctca ttttatcggc tctttccgtt atgtatggat tcttttaatt agtcttcgat
     7801 gtttcttgct tcgttgatac cgctggctaa agattccatt aaggatagtt ctttgtctgt
     7861 aaagctatcc atgtatttct ctatctgtaa tcgtcgggtg ctttttacca agttattagc
     7921 aggtaagaaa aattcatcaa cggaaacatg aagtaacgat acaaggtcat aaagaacttg
     7981 tatgctgggg tgttgccctt tattttcaat attagttaag taccgtgggt caatttcaat
     8041 caatgctccc acttgttcac gagttaaacc tcgtttcaat cgagcttctt taatggctaa
     8101 accaaaggct ctaaaatcat atttatcttc ttttttacgc atagtagacc acctctatac
     8161 attttattgt tcctactgaa ttaaaaacag gtatagaaaa acgtgttata tggtttatag
     8221 gtttatattt aataaaaagc actactaaac gccaataaaa agaaccgtta tatggtagtg
     8281 ctatttacgc tgttaaaata ttgtatatta cttccaaatg gcggtttgtt ggaggtcaac
     8341 gtcgccatga agtacatcat atacaataaa tttccttaca ttgggttctt gtcaaaaaaa
     8401 gtcgtctatc tgcaatagat aagtacgtcc accaatgtgg ttttataaat catatagata
     8461 gaataacaga agcatgtaaa cagagaaata aatctgttta tatgcttttt tggctattca
     8521 gaactttttt acaaagttta tttatcagta atgcaacaaa tccccctttc acattgggac
     8581 taagagtgaa aggagataaa cgagcaaggc tcacttcctt tcctagacag aaagggggtg
     8641 agaaacatga aaccatcttc ttttcagacc acaatagaaa atcagtttga ctatatctgt
     8701 aaacgtgcta tggaagacga gcgaaagaat tatatgcttt atctttcaag gattgcaaag
     8761 cgtgaggtgt ccttttcgga tgttggcgat tatcttgtta gccagtttgc gacaacagat
     8821 aactattcaa ctgactttca gatttttaca ctcaatgggt tatcagtagg cgttgaaaat
     8881 gatttgttga gtgaagcatt acgtgagttg ccagacaaga aacgtgaaat tctactgctg
     8941 ttttacttta tggacatgag cgattcagaa attgcagacc tgttgaaatt gaaccgttct
     9001 actgtctatc ggcatagaac cagtggacta gccttaatta aaaagtttat ggaggaattt
     9061 gaagaatgaa aacacaatat cctatgattc cctttcctct cattgtaaag gcaacagatg
     9121 gcgataccga agcgattaac cagattctac atcattacag agggtacata acgaagcgtt
     9181 ccctacgact tatgaaagat gaatatggca atcaaagtat ggtcgttgat gaagtcttac
     9241 gtggaagaat ggaaaccaga ctgattacaa agattttgtc atttgaaatt aagtaatatc
     9301 ctctctcctt tcgtggaagc gtgctaaacc attccacgct tcccgaacag ggaggtttgt
     9361 tattccacca aagcatattg agctttcaat gtgttttgat aggctaacga gccattgttc
     9421 tttgaaaact gaataaaagt aatcgaatac gtttcgataa gaaaagagcc aacggaacta
     9481 accgccatga cctatcttat aaagatagcg agcgattcat gttagtgatc cgagaagcaa
     9541 tctttagcag gattgcctgc aacgacattc ttatcgtgat aatgatactc ccatacagtc
     9601 aatagtccga gcgtgataaa accgtcgcag gcaatgagta tggctacatg agaaccatgc
     9661 aggggtggaa ctcccgtgag ctttgctaaa gctgttcgat tgctggtaaa acaactttta
     9721 tgaaatccaa ataagtgatt tggaaaggag gattttatga agcagactga cattcctatt
     9781 tgggaacgtt ataccctaac cattgaagaa gcgtcaaaat attttcgtat tggcgaaaac
     9841 aagctacgac gcttggcaga ggaaaataaa aatgcaaatt ggctgattat gaatggcaat
     9901 cgtattcaga ttaaacgaaa acaatttgaa aaaattatag atacattgga cgcaatctag
     9961 cgtcgccaaa gggtcttgta tatgataaaa tagtattaag tcgtatcaag gctctttcca
    10021 taaaggaaag gagcaaatgc catgtcagaa aaaagacgtg acaataaagg tcgaatctta
    10081 aagactggag agagccaacg aaaagacgga agatacttat acaaatatat agattcattt
    10141 ggagaaccgc aatttgttta ctcgtggaaa cttgtggcta cagaccgagt accagcagga
    10201 aagcgtgatt gtatctcact tagagagaaa atcgcagagt tacagaaaga cattcatgat
    10261 ggtattgatg ttgtaggaaa gaaaatgaca ctctgccagc tttacgcaaa acagaacgct
    10321 caaagaccaa aggttagaaa aaacactgaa actggacgca aatatcttat ggatattttg
    10381 aagaaagaca agttaggtgt aagaagtatt gacagtatta agccatcaga cgctaaagaa
    10441 tgggctatta gaatgagtga aaatggttat gcttatcaaa ccatcaataa ctacaaacgt
    10501 tctttaaagg cttcattcta tattgctata caagatgatt gtgttcggaa gaatccattt
    10561 gactttcaac tgaaagcagt tcttgatgat gatactgtcc ctaagaccgt actaacagaa
    10621 gaacaggaag aaaaactgtt agcctttgca aaagctgata aaacctacag caaaaattat
    10681 gatgaaattc tgatactctt aaaaacaggt cttcgtattt cagagtttgg tggtttgaca
    10741 cttccagatt tagattttga gaatcgtctt gtcaatatag accatcagct attgagagat
    10801 actgaaattg ggtactacat tgaaacacca aagaccaaaa gtggcgaacg tcaagttcct
    10861 atggttgaag aagcctatca agcatttaag cgagtgttag cgaatcgaaa gaatgataag
    10921 cgtgttgaga ttgatggata tagtgatttc ctctttctta atagaaagaa ctatccaaaa
    10981 gtggcaagtg attacaacgg catgatgaaa ggtcttgtta agaaatacaa taagtataac
    11041 gaggataaat tgccacacat cactccacat agtttgcgac atacattctg taccaactat
    11101 gcaaatgcag gaatgaatcc aaaggcatta cagtacatta tgggacatgc taatatagcc
    11161 atgacgctga actattacgc acatgcaaca ttcgattctg caatggcaga aatgaaacgc
    11221 ttgaataaag agaagcaaca ggagcgtctt gttgcttagt agtacaaatg aatttactac
    11281 ttatttacca cttctgacag ctaagacatg aggaaatatg caaagaaacg tgaagtatct
    11341 tcctacagta aaaatactcg aaagcacata gaataaggct ttacgagcat ttaagaaaat
    11401 ataaaaagat aattagaaat ttatactttg tttttattaa aaagcagcaa actataaact
    11461 agtaggttcc acactaaatg tagccccata ctgccccata agtccaattt gtagcgtaca
    11521 agccctaaaa acatccccag cgatacatac aaacaccaag ctataatggt tcctggatga
    11581 tgtgctaagg caaataaaac acttgtcaaa gcaactcgaa tatctaattt tctaaccaag
    11641 ttccataaaa tttctctata cagaaattct tcaaccatac tcgcattgat taaaaacaat
    11701 aaaaatgaaa accaaggaac ttgatgttga agcccaatta agtttgcttg attcgtgctt
    11761 tcttgcgcat ggattagact aaaacataga cttataatca gtaggctaac gaatccaata
    11821 ccaagccatt tcatcctaga tttcatattt accttatgag cttgtttgcg ttggccatac
    11881 atccataaaa aagaaatgag tgacgaacca tagagaatct gtagtatagt taactcaccg
    11941 atacaaagaa atttcaataa gtatagagat accaatagga catttacttg ttggaatata
    12001 taaactggaa ttattctttt catagttacc tccgaaataa atcttcataa tctaaatcta
    12061 atacctgcac aatcctttct acccatgaac tttgaggcat tcgttgttcc atcttctagt
    12121 ggcgaatctt ttgatacaaa cgattcaatt cacttggata gtgaaactct cccgcaaaca
    12181 tttttctggt taactcaata cagctgatat ttctttcagc caaaataatg gacaagttct
    12241 cccaaaatcg ttcagccata ttgcttctcc tttagttaga taaataatgt gtttgcgcca
    12301 tgtaaatcaa ttgtttcgta tctcttggca atagagctct agcctcttcc aaattcagac
    12361 ttggataaac tcgcttattt gaaaccgcaa gaggaagtct gatggttagt tcaggatttt
    12421 ttaaaattat ctcaacgaaa tccgttaatc ttagattgtc acggttctta aatcgtaata
    12481 aattgggaga taaaaactca aaacaatctg aagaatagct catcatctca attaatttgt
    12541 cctttgtcat ttcagaaact gaatgacaag atacctctat gccatagttt tggaagaaat
    12601 ctaaaagaag ttgatttctt tgtctatttt tacttagata gagatcaatc atgggagacc
    12661 tcccaaagat tcggttccat ttgatattcc gagacgatta aggaatctaa taaatttgcg
    12721 aagttaatcg gtttcttgtc ttcatcataa gcttttacag ttacttgggt tgtaagtatt
    12781 ccctcttttc cctcggctcg atagccttgt ccatataaaa caaaaacgag attttgatga
    12841 tcatctacaa aggcatcaac cccattcttt atgtcttgac tttcaaggaa ttccataacg
    12901 ttttgaagat aggactcgta aaatagtggg tagttgtgtt ttttatggta atcatctaaa
    12961 aatgttactt caaactcaca tggagagtaa ttttgacttt gaacagccta aaagtgccat
    13021 caaatttgaa ttggaataaa tcaaataaat agccccatcc tcatcaatcc aacctttgct
    13081 caaagacaac tccaaccgat cttttaaaac tgagtaaacc accttaacct ccagtttcat
    13141 attcttatac cgttcactct caaataaaag tttggggagc ttataataac gctctgatgt
    13201 ctgatattga ttagcggtaa tacgcttcat tattgtccct ccaagactaa aattccaaca
    13261 tttccaaatt catcaaatcg gattaaacct acttgttcca tttcatcaac taactgagtt
    13321 gcttttacaa tatcaactcc catgatagtc atgagatgac tcttcacgaa ttgacgtgat
    13381 gactgtcctt ccttttgcat aattacctcc gaaacacaaa aaaaggggta gacaatctag
    13441 tgtctacccc cgaaagttta ttaaaacaaa aatcctgcca aagaattttt ggcaggaaac
    13501 caaatcaatt tatcagtttc tatcaatcgc ttatcgctct caaagactgg taaataggga
    13561 ttccgcaatc aaattgcgat actctattat ttaagagtaa ctgaagctcc agcttcttcc
    13621 aatttagctt tgatttcttc agcttctgca gttgcaacgc cttctttaac aagtgctggt
    13681 gcaccgtcaa caagttcttt agcttcttta agaccaagac cagtgatttc acgtacaact
    13741 ttgataacgc caactttttt gtcgcctgca gatgtcaatt caacgtcgaa tgaatcttta
    13801 gcagcaccag catcagctgc atcagctgca gcaacagcta caggagcagc tgcagttaca
    13861 ccaaattctt cttcgatagc ttttacaagg tcgttcaatt caaggattga agcttcttta
    13921 atttcagcaa taatgttttc aatgttcaat gccattgtta tttcctccaa ataagtttta
    13981 aattttataa tagttttttt cgtagctaga ctacgctgtg tagcttaaga ttaagccgcg
    14041 tcttctttgc tttctgcaac cgctttgact gcaagagcaa cgttgcgcac tggcgcttga
    14101 agtacagaaa ggagcataga aagaagtcct tcgcggtttg gaagagttgc aagtgcaaga
    14161 atctcttctt tagatgcgac agcgccttcg attgcaccac ctttaatttc aagtgcttca
    14221 gcgtttttag aaaagtcgtt caagattttc gctggtgcga taacatcttc attagaaaat
    14281 gctactgcag atggtccaac aaatacagat gcaagatctt caagaccagc tttttcagct
    14341 gcacgacgca agattgagtt tttaataact ttatactcaa cttcgcttcc acgaagctca
    14401 cgacgaagaa ctgtatcttg ctcaactgtc aaaccacgag cgtctacaac gacgatagat
    14461 gcagcagctt tcattttttc agctactacg tcaactagtt ccgctttttt agcaataatt
    14521 gcttcactca ttagtgtgtt cacctccgta attattttgc ttggggaatt tttcaaaaag
    14581 aaaaacgcgc ccaaacctag acacgaaagt acaatacgct tctttttaca tgatacgtct
    14641 tgtcctcggt aggatattta tgagtcgagc tcccctactg tcttaggcag tttttttaaa
    14701 ccgtatataa gtatagcata gtcaaaaaaa gaatgcaaga tttttgcaaa ctttttttaa
    14761 aatttttcgt gattt
//
LOCUS       DAVLSL010000310         7976 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00060772
            SAMN33607233-rid17943763.guided.090, whole genome shotgun sequence.
ACCESSION   DAVLSL010000310 DAVLSL010000000
VERSION     DAVLSL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607233
            Sequence Read Archive: SRR23725673
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721550.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721550.1
            Genome Coverage       :: 260x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:18:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,187
            CDSs (total)                      :: 2,141
            Genes (coding)                    :: 2,018
            CDSs (with protein)               :: 2,018
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 60 of 123
            Pseudo Genes (incomplete)         :: 50 of 123
            Pseudo Genes (internal stop)      :: 39 of 123
            Pseudo Genes (multiple problems)  :: 22 of 123
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607233-rid17943763.guided.090"
                     /strain="AUSMDU00060772"
                     /serotype="07B/07C/40"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2355"
     gene            complement(282..734)
                     /locus_tag="VXN30_002178"
     CDS             complement(282..734)
                     /locus_tag="VXN30_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358704.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYAIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     AKDRSILDNHLDHLLEKMVNDPEKLADFTMDKSLDDAIDEAKSQITFK"
     gene            complement(724..1023)
                     /locus_tag="VXN30_002179"
     CDS             complement(724..1023)
                     /locus_tag="VXN30_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1358705.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLKVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            1696..2010
                     /locus_tag="VXN30_002180"
     CDS             1696..2010
                     /locus_tag="VXN30_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV1358706.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            2026..2412
                     /locus_tag="VXN30_002181"
     CDS             2026..2412
                     /locus_tag="VXN30_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV1358707.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            2441..3826
                     /locus_tag="VXN30_002182"
     CDS             2441..3826
                     /locus_tag="VXN30_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1358708.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            3829..3981
                     /locus_tag="VXN30_002183"
     CDS             3829..3981
                     /locus_tag="VXN30_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV1358709.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            4004..5422
                     /locus_tag="VXN30_002184"
     CDS             4004..5422
                     /locus_tag="VXN30_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV1358710.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMEI
                     RLRSKLKSVLIVHYLKILYNRN"
     gene            5471..5554
                     /locus_tag="VXN30_002185"
     CDS             5471..5554
                     /locus_tag="VXN30_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEV1358711.1"
                     /translation="MLVFQIRNVDKTSTVLKQTKNSDYADK"
     gene            5679..6416
                     /gene="erm(B)"
                     /locus_tag="VXN30_002186"
     CDS             5679..6416
                     /gene="erm(B)"
                     /locus_tag="VXN30_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV1358712.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            complement(6673..7920)
                     /locus_tag="VXN30_002187"
     CDS             complement(6673..7920)
                     /locus_tag="VXN30_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1358713.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
BASE COUNT         2656 a         1406 c         1550 g         2364 t
ORIGIN      
        1 tccatcgtta atcggagatg gactagaatg ctttttgaaa acagatgagt attagaaata
       61 aatgaaaagt caatatcaaa acaatcagaa tcttgatgat gctaaattca aaaaaactac
      121 ataataattt taataagggt aagtcgtacc aagaaacccc accaagataa aaacttagtg
      181 gggtttggcg gggaatataa ttattctccc aattattatc gcctttatat tatctcaact
      241 ctcattaaac aagagttccg caatcaaatt gcgatactct attatttaaa ggtaatttgg
      301 gatttagctt catcaatagc atcatctaat gacttatcca tggtaaaatc agctaatttc
      361 tccggatcat taaccatttt ttctaaaaga tggtctaaat gattgtctaa aatcgaacgg
      421 tctttcgcgt agaaatgtag agaatcccct tgccaagcga tggctaaagg aatctcttct
      481 ttttctaaaa aagctttaaa tttctctata tcaattggtt tgtctaaaaa atcttttttc
      541 agattaatcg tatcaatcgc atagggagat tgtagcaatt cttctaattt ctgcactcct
      601 atcttatagg cggaatcctg tgctaaagcc tgacgtctag accattctag aatctttaaa
      661 agacttttta cagtaaataa aagactacgc tctgcatatt gaactgccat tcgttcctgt
      721 tgttcagagg acatctgatg cctccttctt cacaaatagc agctttcctt ctttataacg
      781 ataagctatc aatttctgac gttgcttaat cgacttgaca accttcagta gatctctcga
      841 ataaggttct cgaagagtga cttctactgc cttcccatca acgataccaa cacgtttaaa
      901 ttcaatgtag tcttttttga gatgtcctaa gcccatacca aaaggaaagt gatgaatcaa
      961 ttggatggta caaccacctt tatttcccat ttgtttcaaa tcatacaagt ttactacctt
     1021 catgattaac tcctttatct agtttgtcgc atcgtttaag tctggtaacg ataaactccg
     1081 tgtctgttag aaaattctca cacacgtctt gtgccagttg cccttcacag ggaaatactc
     1141 tcagtcccta cttacacagg cacgctaatc aagacggagt ggattcaatt ttcaaagaac
     1201 aggtagcttt attatagaga agagtagttg aaatttttat cacatttttt atttttctgt
     1261 agagctaaat tcatactaaa caaagatttt gaaataaaag actaacattg ttacataacg
     1321 ttagcatttc atttccctaa aataagctaa ttttgtctaa aaaatagcat aaaaatctag
     1381 ttatccgcat aaaaactgga cttatcacac tttatcaagg tcaaaaccac tcaatttact
     1441 actaatttac tacttatgaa tgagctttga tacgacgatt tatccttgaa aagtgaagat
     1501 ataaagatac ttccaataaa atttgaatat ttaataggta gacacttcaa aaaatgaggt
     1561 gtctattttt ttacccgatt ttgaaaggaa gtgaacttat gaaaacaaaa aatcaagaat
     1621 caaaaggtcg ttccccactc tttaagacca tcaaacattc attcagccaa taaaaaagaa
     1681 aggataggta aaaatatgga acttaaattt gtgattccca acatggaaaa aacattcggc
     1741 aatttagaat ttgctggcga ggataaagtc gttcagcgaa gaatcaacgg acggctaact
     1801 gtcttatcaa gaagctataa tctctattct gatgttcaaa gagcagatga tattgtggtg
     1861 gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg aggaacgtgt gaagttagtc
     1921 aatccacgta ttaccgcaga gggctacaaa atcggcactc gtggttttac aaattacctt
     1981 ttacatgctg acgacatgat aaaagaataa agaaagagag gaaaaatgat gagattagca
     2041 aatggcattg tattagataa agacacgact tttggagaat tgaaattctc tgctctacgt
     2101 cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag atgaaatcaa ggaacgtacc
     2161 tatgacttaa aatccaaagg acaaggacgc atgattcaag taagtattcc tgccagcgtg
     2221 cctttgaaag agtttgatta taacgcacgg gtggaactta tcaatcccat tgcggacacc
     2281 gttgctactg ccacctatca aggagcagat gttgactggt atatcaaggc agacgatatt
     2341 gtgctgacaa aggattctag ttcattcaaa gctcaaccac aagcaaagaa agaaccgaca
     2401 caagacaaat agtcgctagg tagaaaggag actttttcgc atgaaacagc gtggtaaaag
     2461 gattcgccca tctggtaaag atttagtctt tcattttacg atagcgtcac tcctgcctgt
     2521 tttcctgctg gttgtcggac tgtttcatgt gaagacaatc cagcagatca actggcagga
     2581 ttttaaccta tcacaagcag ataagattga cattccctat ttaattatca gtttcagtgt
     2641 cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa cgggttcgct atgatacggt
     2701 taaacaactt taccaccgtc aaaaactggc aaagatgata cttgaaaaca agtggtatga
     2761 atctgaacag gtcaaaacag agggtttctt taaagatagt gctggtcgta caaaggaaaa
     2821 gataacctac ttccctaaaa tgtattatcg acttaaaaat ggcttgatac agatacgggt
     2881 ggaaatcacg ctgggaaaat atcaagacca actcttacac ttggaaaaga aattagagag
     2941 tggcttgtac tgtgagctga cggataaaga gttaaaggat tcctatgtgg aatatacttt
     3001 gctctatgac accatagcca gtcgtatttc tattgatgaa gtagaagcta aagatggtaa
     3061 acttcgctta atgaaaaacg tatggtggga atatgataag ctccctcata tgttgattgc
     3121 tggtggtaca ggtggcggta aaacttactt tatactgaca ctgattgaag ccttgcttca
     3181 tacagattca aaactgtata ttcttgaccc gaaaaatgct gaccttgcgg acttaggttc
     3241 tgtgatggca aatgtctact atagaaaaga agacttgctt tcttgcattg aaacattcta
     3301 tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg aagaactata agactggcaa
     3361 aaattatgct tacttaggtc tcccggcaca cttcttaatc tttgatgaat acgtcgcttt
     3421 catggaaatg ctgggaacaa aagaaaacac cgcagttatg aataagctga aacagattgt
     3481 catgttaggt cgtcaagctg gcttctttct aatactggct tgtcaacgtc cagacgcaaa
     3541 atatttaggc gacggaatcc gtgatcagtt taatttcaga gtggctttag gtcgtatgtc
     3601 tgaaatgggc tatggcatga tgtttggcag tgacgtacaa aaggatttct tcttaaagcg
     3661 aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc atatcagagt tttatactcc
     3721 ccttgtacca aaaggatatg atttcttgga ggaaattaaa aagttatcca acagcagaca
     3781 gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg gactgatctt gctggctggt
     3841 gtggcaatag ccacgccagc acttaacccc ccgtatctaa caggggggta caaatcgaca
     3901 ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca agggatttac aaaatttcag
     3961 cgtatgtcaa atgggcttta aaagttgaca tacgcctttt tgattggagg gatttttact
     4021 gaatgaacaa acttggttac agcatttaaa agaaaaacgc ttggcttatg gactatctca
     4081 aaaccgttta gctgttgcga ctggtattac aaggcagtat ctaagcgata ttgaaacagg
     4141 aaaagtcaag ccatcagagg atttacagca gtccctttgg gaagctctgg aacgcttcaa
     4201 tcccgacgct ccccttgaaa tgctgtttga ttatgtaagg attcgctttc cgacaacaga
     4261 cgtacagcag gtggtcgaaa acatcttaca actgaaactg tcctattttc ttcatgagga
     4321 ctatggtttc tattcttatt cagagcatta tgctttaggc gacatattcg tcctttgctc
     4381 ccatgaactg gacaaaggag ttctggtgga attgaaaggt cgtgggtgca gacaatttga
     4441 aagctatctt ctggcacaac aaagaagctg gtatgagttc tttatggacg ttttggtggc
     4501 tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat aagacaggga ttttgaatat
     4561 ccctgtactc actgaaaagt gccaacagga agaatgtatc tccgtcttcc gcagttttaa
     4621 aagctatcgc agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg gaaacaccct
     4681 ctatatcggt tcattacaaa gtgaagttta tttctgtatc tatgaaaagg actacgagca
     4741 gtacaagaaa aatgatattc ccattgaaga cgcagaagta aaaaaccgtt ttgagattcg
     4801 attgaaaaat gagcgtgcct attatgcagt ccgtgattta ctcgtctatg acaatccaga
     4861 gcataccgcc tttaaaatta tcaatcggta tatccgtttt gtagataaag acgattccaa
     4921 acctcgttct gattggaaac tgaatgaaga atgggcttgg tttattggga acaatcgtga
     4981 acgattaaaa ctaaccacaa aaccagagcc ttactccttc caaaggacgc tgaactggct
     5041 atctcatcaa gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa tcaaccagac
     5101 gcaggttgta aaagacattc tcgaccatgc gaaactgaca gaccgacaca agcagatttt
     5161 gaagcaacag tcagtaaaag aacaggacgt gataacaaca aaaaaaggat atctgtcaac
     5221 cataccagtt gacagatatc caaaaaaaga tataatggga gataagacgg ttcgtgttcg
     5281 tgctgacttg caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga
     5341 agttatggaa ataagactta gaagcaaact taagagtgtg ttgatagtgc attatcttaa
     5401 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
     5461 gggattcgtc atgttggtat tccaaatacg taatgtagat aaaacatcta ctgttttgaa
     5521 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
     5581 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
     5641 tatttgttta tagatgtaat cacttcagga gagattacat gaacaaaaat ataaaatatt
     5701 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
     5761 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
     5821 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
     5881 cagaaaaatt aaaactgaat actcgtgtca ctttaattca ccaagatatt ctacagtttc
     5941 aattccctaa caaacagagg tataaaattg ttgggaatat tccttaccat ttaagcacac
     6001 aaattattaa aaaagtggtt tttgaaagcc gtgcgtctga catctatctg attgttgaag
     6061 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
     6121 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
     6181 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
     6241 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
     6301 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accattactt
     6361 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     6421 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     6481 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggtat
     6541 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     6601 cctaagtggg atatctgtca actggtatgg ttgactaaaa atacttccta cgaaaatgta
     6661 gggggtattt ttttacgaaa aaatacaatc gattcttaaa aagaaaaatt tttgattggc
     6721 aaaaccataa caagttcgtt ttagggtttt gattttgcga ttgatgcctt ctaaaggacc
     6781 attagagtat tcaaatttag cgctatttaa gacatatttt ctgttttgac gaagggtttg
     6841 aatagcagta tccatttctg tattggtttt ttggtagtct aagatggttg actctagtaa
     6901 ttcactattg cgctcgttta gggctttcgt gatatcttgg taagtttggt atacttcagc
     6961 gaacttggaa aatttactag taatgagatc aacagcattt tggcgagtca tatattgttt
     7021 aacgccgcga agaaaaacta cttcttcagg gtgaagatct tcagcttttt tatggaatag
     7081 cttccaatgt gacttcataa ttttatattc ttggctctgt ttatcaagtt gctttaggat
     7141 agagatacga caattgtcca aagcgcgacc agctaattgt acaaggtgga agcgatcaat
     7201 aatgatattg gcattaggga aaaggcgata gataaaactt tgatattgag catttaaatc
     7261 aattacaact gattgaacgc attcgcgttc ggcttttgaa taacgacttt caaaataatc
     7321 aacaatggta ggtgatagac gatcctgtaa ctttgtgaca atttggtggg tttcagcgtc
     7381 acaacagata aaggacatca cagacttaat tgaacgaaac tcgtcaaaac atagatgctt
     7441 aggcaactta gccacacgat agtgtggttc catgcgctct aagattgttc gacgaacact
     7501 gctaggagag cagtgacaca tttcagcaat aagctgacca gataagcctt tacgagctaa
     7561 aagcatgatt tgatttttga gatcactgga taaggtttga ttttctttgg ttaaattagt
     7621 aatagcacca aaagtagtat ggcatgattt acatttatag cgttgtttac gaagctctag
     7681 ttcatatctt ctcccattta aacttgccag tcgtacatga gttttgcgaa agccatcctt
     7741 attaactgtg ggaaagccac agttacgaca acgattaatc ggataagaaa gagtagctgt
     7801 tattagcgtt atatactctt taacagaatc gttgttgtgt tcagcttctt caacagaaat
     7861 aattttaata tttttatctt taattccaag aatatttagg atagaatcat tatgggacat
     7921 ttgtttaacc ttctttcatg atttttgtgg tgaattgatt gtataacgag gggaca
//
